LOCUS       DAVBBS010000001        83156 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVBBS010000001 DAVBBS010000000
VERSION     DAVBBS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 83156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83156
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.001"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            51..299
                     /locus_tag="VQU32_000001"
     CDS             51..299
                     /locus_tag="VQU32_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471541.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRIYSGNLILQELITVNGAYKATYIGELSSN"
     gene            430..1602
                     /locus_tag="VQU32_000002"
     CDS             430..1602
                     /locus_tag="VQU32_000002"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HET2471542.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENADSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            1602..2876
                     /locus_tag="VQU32_000003"
     CDS             1602..2876
                     /locus_tag="VQU32_000003"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HET2471543.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLEFANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLIGILSNYATDSL
                     VTASCRMAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VAFSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(2912..3877)
                     /locus_tag="VQU32_000004"
     CDS             complement(2912..3877)
                     /locus_tag="VQU32_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2471544.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQTIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(3858..5312)
                     /locus_tag="VQU32_000005"
     CDS             complement(3858..5312)
                     /locus_tag="VQU32_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET2471545.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKSQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEHPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            5689..7572
                     /locus_tag="VQU32_000006"
     CDS             5689..7572
                     /locus_tag="VQU32_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2471546.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYALADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVSKGDAVIE
                     VKI"
     gene            7721..8608
                     /locus_tag="VQU32_000007"
     CDS             7721..8608
                     /locus_tag="VQU32_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2471547.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGTSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(8928..9545)
                     /locus_tag="VQU32_000008"
     CDS             complement(8928..9545)
                     /locus_tag="VQU32_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HET2471548.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(9960..10148)
                     /locus_tag="VQU32_000009"
                     /pseudo
     CDS             complement(9960..10148)
                     /locus_tag="VQU32_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(10658..10738)
                     /locus_tag="VQU32_000010"
                     /pseudo
     CDS             complement(10658..10738)
                     /locus_tag="VQU32_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            10854..11747
                     /locus_tag="VQU32_000011"
     CDS             10854..11747
                     /locus_tag="VQU32_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2471549.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            11744..12943
                     /locus_tag="VQU32_000012"
     CDS             11744..12943
                     /locus_tag="VQU32_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2471550.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLEQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(13296..14063)
                     /locus_tag="VQU32_000013"
     CDS             complement(13296..14063)
                     /locus_tag="VQU32_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471551.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(14080..14775)
                     /locus_tag="VQU32_000014"
     CDS             complement(14080..14775)
                     /locus_tag="VQU32_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2471552.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            complement(14780..15145)
                     /locus_tag="VQU32_000015"
     CDS             complement(14780..15145)
                     /locus_tag="VQU32_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2471553.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMIH
                     FGYEKEELPGVVSDYIKGV"
     gene            15285..15758
                     /gene="nrdR"
                     /locus_tag="VQU32_000016"
     CDS             15285..15758
                     /gene="nrdR"
                     /locus_tag="VQU32_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HET2471554.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            15759..16928
                     /locus_tag="VQU32_000017"
     CDS             15759..16928
                     /locus_tag="VQU32_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HET2471555.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFNPKEATIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRER
                     GQKTKTQKQNQTAPAKTNIPKWSNPDYKNETSEETRLELERKKQELLARLEKGGD"
     gene            16929..17825
                     /gene="dnaI"
                     /locus_tag="VQU32_000018"
     CDS             16929..17825
                     /gene="dnaI"
                     /locus_tag="VQU32_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HET2471556.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            17822..18535
                     /locus_tag="VQU32_000019"
     CDS             17822..18535
                     /locus_tag="VQU32_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HET2471557.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            18549..19859
                     /gene="der"
                     /locus_tag="VQU32_000020"
     CDS             18549..19859
                     /gene="der"
                     /locus_tag="VQU32_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET2471558.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            19950..20285
                     /locus_tag="VQU32_000021"
     CDS             19950..20285
                     /locus_tag="VQU32_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471559.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            20425..20760
                     /locus_tag="VQU32_000022"
     CDS             20425..20760
                     /locus_tag="VQU32_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471560.1"
                     /translation="MEYLFKMIILLPCFYFFSWIEKANRDSKIFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGGRH"
     gene            20945..21736
                     /locus_tag="VQU32_000023"
     CDS             20945..21736
                     /locus_tag="VQU32_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471561.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLEWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYVVPYMVLKKYEDNV"
     gene            21729..22370
                     /locus_tag="VQU32_000024"
     CDS             21729..22370
                     /locus_tag="VQU32_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471562.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLIVSRLEEKFKKG"
     gene            22374..23024
                     /locus_tag="VQU32_000025"
     CDS             22374..23024
                     /locus_tag="VQU32_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2471563.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            23035..23322
                     /locus_tag="VQU32_000026"
     CDS             23035..23322
                     /locus_tag="VQU32_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471564.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     MQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            23468..25981
                     /gene="secA"
                     /locus_tag="VQU32_000027"
     CDS             23468..25981
                     /gene="secA"
                     /locus_tag="VQU32_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET2471565.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMATLTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQAMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            26062..27093
                     /locus_tag="VQU32_000028"
     CDS             26062..27093
                     /locus_tag="VQU32_000028"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET2471566.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            27095..28126
                     /locus_tag="VQU32_000029"
     CDS             27095..28126
                     /locus_tag="VQU32_000029"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET2471567.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            28165..28533
                     /locus_tag="VQU32_000030"
     CDS             28165..28533
                     /locus_tag="VQU32_000030"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET2471568.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            28523..29626
                     /locus_tag="VQU32_000031"
     CDS             28523..29626
                     /locus_tag="VQU32_000031"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET2471569.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLALPYDLIPALTLESALVHIKTVPAGACMGYGATYQTDSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            29645..31660
                     /gene="recG"
                     /locus_tag="VQU32_000032"
     CDS             29645..31660
                     /gene="recG"
                     /locus_tag="VQU32_000032"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET2471570.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     IKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            31976..32956
                     /locus_tag="VQU32_000033"
     CDS             31976..32956
                     /locus_tag="VQU32_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HET2471571.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            33761..33988
                     /locus_tag="VQU32_000034"
     CDS             33761..33988
                     /locus_tag="VQU32_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471572.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            34276..37173
                     /gene="nanA"
                     /locus_tag="VQU32_000035"
     CDS             34276..37173
                     /gene="nanA"
                     /locus_tag="VQU32_000035"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HET2471573.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGNRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHNGKEYIILSNAGGPNRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
     gene            38188..38640
                     /locus_tag="VQU32_000036"
     CDS             38188..38640
                     /locus_tag="VQU32_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2471574.1"
                     /translation="MIITKISHLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVQLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            38659..39996
                     /locus_tag="VQU32_000037"
     CDS             38659..39996
                     /locus_tag="VQU32_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET2471575.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            40075..40959
                     /locus_tag="VQU32_000038"
     CDS             40075..40959
                     /locus_tag="VQU32_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2471576.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            40959..41792
                     /locus_tag="VQU32_000039"
     CDS             40959..41792
                     /locus_tag="VQU32_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2471577.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            41810..43903
                     /gene="nanB"
                     /locus_tag="VQU32_000040"
     CDS             41810..43903
                     /gene="nanB"
                     /locus_tag="VQU32_000040"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HET2471578.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGESQTVVMKFKADEPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKRSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNREESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            43915..45018
                     /locus_tag="VQU32_000041"
     CDS             43915..45018
                     /locus_tag="VQU32_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2471579.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            45192..45890
                     /locus_tag="VQU32_000042"
     CDS             45192..45890
                     /locus_tag="VQU32_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2471580.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            45909..47426
                     /locus_tag="VQU32_000043"
     CDS             45909..47426
                     /locus_tag="VQU32_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2471581.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFLGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            47551..48879
                     /locus_tag="VQU32_000044"
     CDS             47551..48879
                     /locus_tag="VQU32_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2471582.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            49131..49997
                     /locus_tag="VQU32_000045"
     CDS             49131..49997
                     /locus_tag="VQU32_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2471583.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            50013..50852
                     /locus_tag="VQU32_000046"
     CDS             50013..50852
                     /locus_tag="VQU32_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2471584.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            51125..51577
                     /locus_tag="VQU32_000047"
     CDS             51125..51577
                     /locus_tag="VQU32_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2471585.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEATDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPTGYAGMEEKVRKYLFKILID"
     gene            51674..52018
                     /locus_tag="VQU32_000048"
     CDS             51674..52018
                     /locus_tag="VQU32_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471586.1"
                     /translation="MVSSKHAARASFLDGQEITVDEMSWIINGIVNALIGRYIKLGTY
                     AAKYGIGIARSILSRAAATAAARVGLLTKISGWILRVAVNVADVYGNFANNIAAAWDA
                     YDKIPNNGRINF"
     gene            52302..52946
                     /locus_tag="VQU32_000049"
     CDS             52302..52946
                     /locus_tag="VQU32_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET2471587.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            53115..54032
                     /locus_tag="VQU32_000050"
     CDS             53115..54032
                     /locus_tag="VQU32_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET2471588.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTATHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKESFL"
     gene            54050..54934
                     /locus_tag="VQU32_000051"
     CDS             54050..54934
                     /locus_tag="VQU32_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2471589.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(54950..55801)
                     /locus_tag="VQU32_000052"
     CDS             complement(54950..55801)
                     /locus_tag="VQU32_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2471590.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANRVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            56001..58058
                     /gene="pbp2b"
                     /locus_tag="VQU32_000053"
     CDS             56001..58058
                     /gene="pbp2b"
                     /locus_tag="VQU32_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HET2471591.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTKRQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEINKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            58069..58665
                     /gene="recR"
                     /locus_tag="VQU32_000054"
     CDS             58069..58665
                     /gene="recR"
                     /locus_tag="VQU32_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HET2471592.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            58841..59884
                     /locus_tag="VQU32_000055"
     CDS             58841..59884
                     /locus_tag="VQU32_000055"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET2471593.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            59968..61341
                     /locus_tag="VQU32_000056"
     CDS             59968..61341
                     /locus_tag="VQU32_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET2471594.1"
                     /translation="MKLTIHEVAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGVRDGHDFIERAFENGAAATLSEKEVANHPYILVDDVLTAFQQLAAYYLEKTAVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQVLDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGVQSVQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVE
                     SLENEDK"
     gene            61328..61939
                     /locus_tag="VQU32_000057"
     CDS             61328..61939
                     /locus_tag="VQU32_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2471595.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRDLLSEML
                     NQASDLDSEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDPLPNLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            62030..62734
                     /locus_tag="VQU32_000058"
     CDS             62030..62734
                     /locus_tag="VQU32_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HET2471596.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            62953..64326
                     /gene="ftsA"
                     /locus_tag="VQU32_000059"
     CDS             62953..64326
                     /gene="ftsA"
                     /locus_tag="VQU32_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET2471597.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENIIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVESVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            64343..65602
                     /gene="ftsZ"
                     /locus_tag="VQU32_000060"
     CDS             64343..65602
                     /gene="ftsZ"
                     /locus_tag="VQU32_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET2471598.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            65607..66278
                     /locus_tag="VQU32_000061"
     CDS             65607..66278
                     /locus_tag="VQU32_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET2471599.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKATQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            66288..66827
                     /locus_tag="VQU32_000062"
     CDS             66288..66827
                     /locus_tag="VQU32_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HET2471600.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            66827..67090
                     /locus_tag="VQU32_000063"
     CDS             66827..67090
                     /locus_tag="VQU32_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET2471601.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            67087..67771
                     /locus_tag="VQU32_000064"
                     /pseudo
     CDS             67087..67771
                     /locus_tag="VQU32_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            67780..68568
                     /gene="divIVA"
                     /locus_tag="VQU32_000065"
     CDS             67780..68568
                     /gene="divIVA"
                     /locus_tag="VQU32_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HET2471602.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            68916..71708
                     /locus_tag="VQU32_000066"
     CDS             68916..71708
                     /locus_tag="VQU32_000066"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET2471603.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYANFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEEPAPEAALSFEDVVFTVERAAGEVCDRCRRIDPTTAERSYHAVICDHCASI
                     VEENFADAVAEGFEEK"
     gene            72118..72480
                     /locus_tag="VQU32_000067"
     CDS             72118..72480
                     /locus_tag="VQU32_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471604.1"
                     /translation="MKSSGIVGLVGGILLLICPFVLLGTAVNTAATTLNGGATAGAFS
                     GVALLLNALKIANLVLGIIAIVYYKGDKHVGAAPSVLMIVSGEVSLIPFLGWVGGILA
                     IIGGSLFLATLKKFKSEE"
     gene            73015..73707
                     /locus_tag="VQU32_000068"
     CDS             73015..73707
                     /locus_tag="VQU32_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HET2471605.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            73760..76171
                     /locus_tag="VQU32_000069"
     CDS             73760..76171
                     /locus_tag="VQU32_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HET2471606.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     RKAFASFPDDLLIQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYSGNLFSY
                     KGQEYIEAARAILNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            76421..77122
                     /locus_tag="VQU32_000070"
     CDS             76421..77122
                     /locus_tag="VQU32_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2471607.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIQMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            77124..79824
                     /locus_tag="VQU32_000071"
                     /pseudo
     CDS             77124..79824
                     /locus_tag="VQU32_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
     gene            complement(79920..80411)
                     /gene="tpx"
                     /locus_tag="VQU32_000072"
     CDS             complement(79920..80411)
                     /gene="tpx"
                     /locus_tag="VQU32_000072"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HET2471608.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(80534..81463)
                     /gene="psaA"
                     /locus_tag="VQU32_000073"
     CDS             complement(80534..81463)
                     /gene="psaA"
                     /locus_tag="VQU32_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HET2471609.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(81495..82343)
                     /locus_tag="VQU32_000074"
     CDS             complement(81495..82343)
                     /locus_tag="VQU32_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET2471610.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(82340..83062)
                     /locus_tag="VQU32_000075"
     CDS             complement(82340..83062)
                     /locus_tag="VQU32_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET2471611.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT        25138 a        15160 c        18365 g        24493 t
ORIGIN      
        1 tactttcctt attgtctgta aatctactga ccttgttgtt tatcccagtc atggtttcta
       61 gttcgggctc agagtttcaa agtggatggc aagagcatca attgattgct gagaaggtta
      121 gtaaaacact tgataagaca tttgataagg atgtcagaga aattccgacc agtcagtttt
      181 atcaaaaatt tgtagatgag atgggaagga tttactcagg aaatttgatc ctccaggagc
      241 tgataactgt gaatggagct tataaagcta cttatatcgg tgaactctct agcaactaat
      301 cattctttaa aatgatgacc aataagaggc ttgatttggt gaatcaagtc ttttttctct
      361 cttttactgc catttgtgat aaaatagtat agaacgattt tttacatgaa tgataaaaca
      421 gaggtaaata tgacaatcgg tattgataag attggttttg cgaccagtca atatgtcttg
      481 aaattacaag acttagcaga agcgagggga attgaccctg aaaaattaag taaaggactc
      541 ttactcaagg aattgagtat tgcgccccta actgaggaca tcgtgacctt ggcggccagt
      601 gctagtgact ctattttaac tgagcaagaa agacaagaag ttgacatggt cattgtggct
      661 accgagtcag gaattgacca gagtaaggct gcggccgtct ttgtgcatgg cttgctgggc
      721 atccagccct ttgctcgtag tttcgagatt aaagaagcct gctatggggc gacagctgcc
      781 ctccattatg ccaaattgca tgtggaaaat tctccggagt ccaaggtctt ggtcattgcc
      841 agtgatattg ccaaatatgg tattgaaact ccgggagaac caactcaagg tgctggaagt
      901 gtagctatgt tgattacaca aaatccacgc atgatggcct ttaataatga caatgtagct
      961 cagacccgtg acatcatgga tttctggcga ccaaattact cgacaactcc ttatgtaaat
     1021 ggtgtctatt ctacccaaca atacttggat agtttgaaaa cgacttggct tgaatatcaa
     1081 aaacgctacc agcttactct ggatgatttt gcggctgttt gtttccactt gccttatcct
     1141 aaattagcgc taaaaggctt gaaaaaaatc atggataaga gcctgcctca agagaaaaaa
     1201 gacctcttac aaaagcattt tgaccagtct attctctaca gtcaaaaggt ggggaatatc
     1261 tacacaggtt cacttttcct tggacttttg tctctcttgg aaaatgcaga tagcttgaaa
     1321 gctggggata aaatcgccct ttatagttac ggaagtggag ctgtggctga gttcttcagt
     1381 ggtgaattgg ttgaaggata tgaagcttat ttggataaag accgcttgaa caagctcaac
     1441 caacgaactg ccttatccgt tgcagactat gaaaaggtct tttttgagga agtaaacttg
     1501 gatgaaacaa actctgccca gtttgctggc tatgaaaatc aagattttgc cttggttgaa
     1561 attctcgacc accaacgccg ttatagcaag gttgaaaaat aatgaagata agttggaatg
     1621 gattttctaa aaaatcatac caagagcgcc tcgagctgtt aaaagctcag gcgctcctta
     1681 gtcctgagag acaagctagt ctggagaagg atgaacagat gagtgtgact gtggcagacc
     1741 agctgagtga gaatgtagtg ggaacttttt ctctgcctta ttcgctggtt ccggaggtac
     1801 ttgtcaacgg tcaggaatac accgttccct atgtgacaga agaaccctct gtggttgcgg
     1861 cggccagcta tgccagcaaa atcatcaagc gtgcaggtgg ttttactgca caagtccatc
     1921 agcgccagat gattgggcag gtagcccttt atcaagttgc taatcctaaa ctagcgcaag
     1981 agaagattgc cagcaagaaa gcggagctct tggagtttgc caatcaagcc tatccttcta
     2041 tcgttaaacg tggaggtggg gcgcgtgatc tgcatgtcga gcagataaaa ggcgaaccag
     2101 actttctcgt tgtttatatt catgtcgata cccaggaagc catgggtgcc aatatgctca
     2161 acaccatgct ggaagccttg aaaccagtct tagaagaact cagtcaggga cagagtctca
     2221 taggaatcct gtccaactac gcgaccgatt ctctggtgac tgcaagctgt cgcatggcct
     2281 ttcgctactt gagccgccaa aaggatcaag gacgagagat tgcggagaaa attgcgttgg
     2341 ctagtcagtt tgcgcaggct gatccttacc gagctgctac tcataataaa ggaattttta
     2401 atggtattga tgcgattttg attgccactg gtaatgactg gcgtgccatc gaagctgggg
     2461 cccatgcctt tgccagtcga gatggacgct atcaaggtct tagttgctgg acgctggacc
     2521 ttgaaagaga agaattggtc ggtgagatga ccctgcccat gcctgtagcg actaagggtg
     2581 gctctatcgg cctcaaccca cgtgtagctt tcagtcatga tctactagga aatccttctg
     2641 ccagagaatt agcccagatt atcgtgtcca tcggtcttgc ccaaaatttt gcagccctca
     2701 aagctttggt aagtacgggc atccagcaag gccacatgaa actacaggcc aaatccctag
     2761 ctctcctagc tggggctagt gaatctgaag ttgctcccct agtagagcgc ctcatctcag
     2821 ataaaacctt taacctagag acagcccagc gctatctcga aaatttaaga tcataaaaaa
     2881 ctcagacgaa tcggtctgag ttttcttgtg tttaaatact ttttcctggt aatagtgtaa
     2941 ctggtaagaa gtaaccagtt gtggcaactt ccttgccttc aatcttttgc aagagaagat
     3001 caatagtgag acaagcaatc tcttccaaag gttgcttgat agtagccaat tgagggtagt
     3061 aattttcgat aaagtaggtc ccatcatagc cgatgacctt gagctctttt gggacagaaa
     3121 tgcccaattc ttgagcgatt ttaatgacca gaatagctgt caaatcatcc gaagcaaaaa
     3181 tggcatctgg tttttcccgg gtcaagatat tcttgatttc catttctttt ctgacgggag
     3241 aaaagtcact ggaaacattg ataataggag cttttgggag tacggatgca aaaccagcgt
     3301 ggcgtagtcc ggttggcgaa ttggaattgt cattccctgt aatcatgatg atagtctggg
     3361 cacctgtctt gaccaaggtt tgggcagcaa gaaccccacc agcatagttg tcagaggaga
     3421 cgacagggat gtctggcgat aggtttcggt caaaggaaat aatcggcgct gtcacacgat
     3481 tgtagtcttc gattcctagg ttgtgactac cagaaatgat gccgtccacc tgattggctt
     3541 ccaacatttc gatgtattcg cgttccttct cagaatcatg ttcactgttg cagatgatgg
     3601 tcttgtaacc atttttgaag agttggtgtt ccaatttatc aatcaattct gcatagaaaa
     3661 cattggaaat attagggaaa atcaagccga ttaacttagc tgattttcct tgcagactac
     3721 gagccaggtt gttgggttta tagcccaatt ctcgcatggc ttcattgact ttttggatgg
     3781 ttttctcaga tagataccct tttttattga taacccgaga aacggtagta ggactgacgc
     3841 ctgcaagttt ggcgacatca gttagttttg cgaccataat ctaattcata gtaagttcca
     3901 gttgggtttc cagatttgat caggatacca ttttggtccg catgtgggaa gacacgacca
     3961 gaaaatactt tttctccttt attgatgaaa atttcaaaga cagagttatc gatgaagatt
     4021 gtagcagtag tagcctgatt ctcgataggg caagaacgag ttgtcccaaa ttcttgggca
     4081 tactgttctc cagcctggct acgatccact gtcacttgac cgtttacaag gtcaaagttg
     4141 attgaaagtc ccttaccttc tttatcagca agtaagacaa tctcgctctg gctattagct
     4201 tccaagttga gttcaagttc gtaagtgttc ttggtttggg aacggtttga gaaggcttct
     4261 tcagaagcac gaaggtcctt aatagcagcg actggatact ggtagagctt gtcgtctttg
     4321 atagtgagtt ccttgaccaa agagaaggtt ccttggtggt caaaacggtc agatgggtaa
     4381 gaaacatctg gcaaaccaag ccagctaact gctagagcac gcccatcagg agcgttgaag
     4441 gcttgagttg cataggcttc gaaaccgtaa tccatgtttt gaagttgaga cacatctacc
     4501 attttggcat ttttagggtc aaaggaagcc ccaatcttat acatatttgg aaagatatta
     4561 tcgtagtcta gaactttctt atccaatcct tgtggacagt agagaaggac aggatgttcc
     4621 tctacaaaga ccagattagg acattccatc atgtaggcag tacggtcgtt agcaaagtca
     4681 aggtcgccaa ctgcttgcca gtttgtgtag tcgttattga cagccttgta gagacggacg
     4741 aagccttttt tctccaagtc ctgtccaccg acgacagcat agtattgact tttaaagtta
     4801 aaaatttgtg gatcgcggaa gtggtcagta gagtctgctg gctggtcaat caagatcttg
     4861 tcaatctttg taatcttacc ctccttgtcc atcaaagcac cgatctggta tgggtgacgg
     4921 atccagtttt catcgcgaac atttcctgta taaaatagga ataagttatc gccaaattgc
     4981 atggcagaac cagagtaggc accgtggcta tctaatggag tatctggtaa aactttgatt
     5041 ccagtttctt taaagtgaac caaatcatca ctttctagct gtgcccaaga tttcaaaccg
     5101 tgggctgcac caaaaggaaa attctggtaa aagaggatcc acttgccatc aaagtaagaa
     5161 aagccatttg ggtcgttgag aagtcctgtt tttggctcaa catggtaatg agtatgccat
     5221 ggagattgtg ccatattttc ctttatatgt tgaatttctt cttgcgtcca atcttgataa
     5281 agtctgtaac gacgctcagt tgtccattcc attcattcct ccaaaaatct tatcactttt
     5341 aatagtaact gttttcgata aaaaatgcaa gccttttatg ttaaaaaaga ggaaaaactg
     5401 tcaaacgatt atcataaaat aaatacagga aatcaatcaa atttttacga aaatgtgaaa
     5461 gataatgaaa taaaagactt tttaaccaag gtttcaaact tatttttcaa ggaattcctg
     5521 atgaaacagt caaaaacaat caatctaaaa cttgcccact aagtggatag aatatttttt
     5581 ctgcaaaacg cttgacatat ttttaaaaca tgataaaata atagtcgtaa gggcgaataa
     5641 aatcgctttc aaacaagaac aaaatgttat ataaggagat ttttgcaaat gaacaatcag
     5701 gaaattgcaa aaaaagtcat cgatgccttg ggcggacgtg aaaatgtcaa tagtgttgcc
     5761 cactgtgcga ctcgtctacg tgtcatggtc aaagatgaag agaaaatcaa taaagaagtg
     5821 attgagaact tggaaaaagt tcaaggtgct ttctttaact cagggcaata ccaaattatc
     5881 tttggtacag gtacagttaa caaaatgtac gatgaagttg ttgtacttgg cttaccaaca
     5941 tcatctaagg atgacatgaa agcagaagtt gctaaacaag ggaactggtt ccaacgtgct
     6001 atccgtactt tcggtgatgt tttcgttcca atcatcccag ttatcgtagc gacaggtctc
     6061 ttcatgggtg tgcgtggtct tttcaacgct cttgaaatgc cacttccagg tgactttgca
     6121 acttacacac aaatcttgac agatacagcc ttcatcatct tgccaggttt ggttgtgtgg
     6181 tcaaccttcc gtgtatttgg tggaaatcct gccgttggta tcgttcttgg tatgatgctt
     6241 gtctctggct cacttccaaa cgcttgggca gttgctcaag gtggtgaagt aacagcgatg
     6301 aacttctttg gtttcatccc tgttgttggt ttgcaaggtt ccgttcttcc agccttcatc
     6361 atcggggttg tcggagctaa atttgaaaaa gctgtccgca aggttgttcc agatgtcatt
     6421 gacctcttgg taacaccatt cgtgacactt ttggtcatgt ctatccttgg actctttgtc
     6481 attggaccag ttttccacgt tgttgaaaac tacatcctta ttgctacaaa agcgattctt
     6541 agcatgccat ttggtcttgg tggtttcttg attggtgggg ttcaccaatt gatcgtcgtg
     6601 tcaggtgtgc accacatctt caacttgctt gaagtgcaat tacttgctgc tgaccatgct
     6661 aacccattca acgctatcat cacagctgct atgacagctc aaggtgctgc tactgttgcg
     6721 gttggtgtta aaacaaaaaa tccaaaactg aaaacacttg ctttcccggc tgctctttct
     6781 gccttcctag gtattacaga gcctgctatc ttcggggtga acttgcgctt ccgtaaacca
     6841 ttcttccttt cattgattgc tggtgcaatc ggtggtggat tggcttctat ccttggactt
     6901 gctggtactg gtaatggtat caccatcatc cctggtacaa tgctttatgt tggtaacgga
     6961 caacttccac aataccttct tatggtagct gtatcatttg cccttggttt tgctcttact
     7021 tacatgtttg gttacgaaga tgaagtagac gcaactgcag ctgcaaaaca agctgaagtg
     7081 gctgaagaaa aagaagaagt tgcgccagca gctcttcaaa atgaaacact tgtaactcct
     7141 atcgtcggtg atgttgtcgc tcttgctgat gtcaatgacc cagtcttctc aagtggagct
     7201 atgggacaag gtatcgctgt gaaaccaagt caaggcgtgg tctatgcact agctgatgct
     7261 gaagtttcaa ttgcctttcc aacagggcac gcttttggtt tgaaaacaag aaatggtgct
     7321 gaagttttga ttcacgttgg tattgacact gtatctatga acggtgacgg ttttgaagca
     7381 aaagttgctc aaggtaataa ggtgaaagct ggcgatgttc ttggaacatt tgactcaaac
     7441 aaaatcgctg cagctggact tgatgataca acaatggtta tcgttacaaa tacagctgac
     7501 tacgcttcag tagctccagt cgcaacaggt tcagtttcga agggggatgc tgtgatcgaa
     7561 gtgaaaatct aatcaatcct ctctaatgtg aaaacgaaca aatgacatgt ttgttcgttt
     7621 ttattagata gtaagattta cagaggaaaa tctaaaaaat agagacattt agactttcga
     7681 agtatgctat aataaagaaa ataaaaacaa gaggtttatc atgacaaaat tatatggaag
     7741 cttggaagcg ggcggtacaa agtttgtctg tgctgtcggt gatgaaaact ttaacgttgt
     7801 agaaaaaaca caatttccaa caacaactcc aatcgaaaca atcgataaaa ccattgagtt
     7861 cttctcaaaa ttcgataacc ttgctggtct tgcagttggt tcatttgggc cgattgatat
     7921 tgacaaaaac tcaaaaactt atggctttat cacgacgact ccaaaaccaa actgggcaaa
     7981 tgtggacttg cttggtgccc ttcgtcgcgc cctaaacgtg ccaatgtact tcacaacaga
     8041 cgtaaacagc tctgcttacg gtgaaatggt tgctcgtaac aatgctggtg gtcgtatcga
     8101 aaacttggtt tactacacaa tcggtacagg tatcggtgca ggcgtcatcc aacgtggtga
     8161 gtttatcggt ggtgtgggtc accctgaaat gggtcattat tatgttgcta gacacccaat
     8221 ggatattgaa aaagagttta agggtgtttg tcctttccat aagggatgtc tagaaggtta
     8281 tgcagctggt acaagtttgg aagctcgtac aggtgtacgt ggggaaacta ttgaactcaa
     8341 caaccctgtt tgggatgttc aagcctacta tatcgctcaa gctgcggtta atgcgacagt
     8401 gactttccgc ccagacgtga ttgtctttgg tggaggggtc atggctcaac aacatatgct
     8461 ggaccgtgtc cgtgagaaat ttacatctct tcttaatggt tacctaccag taccagatgt
     8521 gcgtgactat atcgtgacgc cagcagtcgc aggaaatggt tctgccacac ttgggaactt
     8581 tgttcttgca aaagaagttt caaaataaag cacaaaatcc gcttgggaaa ccaaacggat
     8641 tttgtggttg aaaacaatat ttttgagcct ttccttaagt gattacggac ggtagcgact
     8701 ttcttcgaaa ttccatacct aaactttgag cctagtgtct caaagtttcc gaatacctga
     8761 aaccattaga tttcaggtgt ttctatcacg gcggaaaggc taggtaagtc ctcgcctcac
     8821 tatcgaaaaa tatgcataat ttccatacca aagtatattt agtatcaagc tatagttaat
     8881 ctacgcttta aaaatccact tgggaaacca aacggatttt ttacgtgtca aagcatttgc
     8941 cagaaaaagt caataatata ggtcagaccg taggtataaa ccacgagggt aaagggtttg
     9001 gtcagaatga tgatagtcat gtagcgcttg aaactcatct tggtcagggc ggccagcata
     9061 cagagaaagt cagctgggct aatgggccaa atcatcataa aaataaagaa gcggtcaaaa
     9121 cgattgccct tatctagcca gccgatgtac ttgtcgtagg tgcgcttgct gacgacagac
     9181 tggacaaagg cagctccgta taggcgcact agataaaaga taatggcaca gccaatcacg
     9241 atgccgatat agttgtagat agtcccgatg atgtgcccgt agataaagac cccagccacc
     9301 gaggtcaagg cccctggaat gatagggacg acagtctgta aaatctgtaa aaagataaag
     9361 agaggtggac cccagatgcc tgcctgctgg ataaaggcag agagggtttc cttggattgt
     9421 aaaatcccag cctgataagc ccaaatacag aaaatcaaac tcccaacccc accgacaatc
     9481 gatgagatat tgataactcg ctgcagaagg ggagaaacag ctttcatttg tttatcctgt
     9541 gacatgtaaa ctcctcatag atagtatatt tctactatac catattttgg aggagaaagg
     9601 gggaatgagt cttttttgat aaggaataga aagtaaatag cccttcgaga cttagaatag
     9661 gtctgtcaac taaaggttat atgcattcta ggtttcgaat aatcgcaatt ccataaattt
     9721 aaaagtattc taagtttcga atagcttatc tgagataatt ttgaggcatt cgcgctttcg
     9781 aatagtcatt ttccctaaag tttatatgta ttctgaagct agaaagacta aaatataaaa
     9841 gtagtaagag tctctctctg atggacgtaa tttatggcta gtatgaatcc tttgaattgt
     9901 tagttaattt taatattaat cagttctgaa ataattacta aatatagtta gatggtgagt
     9961 taattattgc taaatttcaa gttcaagtta gtcatccaga agtcttctct gggggacttg
    10021 tagttcaagc attttttagg atagttgtta atccactttt cgataaatgc gacttccttt
    10081 gtagttgttt tcttagttcc ctgaggtaac catctatgaa tgagcctgtt gtgattttca
    10141 ttagatccta tagtgatgtg cataatagat atagcagatt gaaactagaa tagtatacat
    10201 ctacttctaa aacattgtta gaaatcaatt tgactgtcct gatcaatttg tcctgttttt
    10261 atttcatttt actatataat ccttagaaaa catatcggac aagcggttga attccgttcc
    10321 gtccattagt agataaagga ttactccttc ttgaaatgac agatgaactt gtttcttata
    10381 aaaatgttgt ctgaaactat tagccttttt ccagaagttt tagaggaaga aaactttacg
    10441 aggaaaaagg agttactcaa ataacaattc aggattaaaa atagacagtt gaggagcgga
    10501 aggtataaat taagtttgct actgtataat ggatttatca ctaatactct tcgaaaatct
    10561 cttcaaacca cgtcagcttc gccttgccgt agatatagtt actgacttcg tcagttctat
    10621 ccacaacctc aaaacagtgt tttgagctga cttcgtcagt tttatctgca acctcaaagc
    10681 tgtactttga gcagcctacg gctagcttcc tagtttgctc tttgcttttc attgagtata
    10741 aaagtgctcc aggccatatt ttacacagca atagtcttct aaaaatggac aaaaacattg
    10801 taaaggctat caaaaaggag tataatgagt gcaagaaatt tcggaggtga aagatgctag
    10861 aagtaagaag tctagagaaa agttttggct ccaagcaagt tttgtttggt attgactttc
    10921 aagcgcgacc aggtcgtatt ttgggactag tcggaaaaaa tggtgctgga aaaacaacga
    10981 ttttccatag tattttgaaa ttcctagaat atcagggaga aatcggtctg gatggtcagg
    11041 atattcgtca ggagacctat gctcggattg gttatctgcc tgaagaacgc agtctcatgc
    11101 ctaaattgac agttcttgaa caagttcgct acttggcgac tctaaaaggc atggatgcca
    11161 aagaagtcaa agaaaaactc cctcaatgga tgaagaggtt ggaagtgaaa ggaaagctga
    11221 cagataaaat caagagtctg tcaaaaggaa atcagcagaa gattcagctc attattactc
    11281 tgattcatga accagacctg attatcttgg atgagccttt tagtggattg gacccagtta
    11341 atacagaatt gctcaaacaa gtcatttttc aggaaaaaga gcgcggagca accattatct
    11401 tttctgacca tgtcatgacc aatgttgagg aactttgtga cgatattctg atgatccgag
    11461 atggccgtgt ggtcttgcac ggaccagttc aggatgtccg caatcaatac gggaaaacgc
    11521 gtctctttgt ttcaagtgaa cgaagcaagg aagaattgga aaatcttcct catgtcaaac
    11581 aggtgagctt gaccaaacaa ggcagttgga aattgatttt ggaggatgag agcgctggaa
    11641 gggaactctt cccaatcttg actcaaggtc aatatatcgc aacatttgac caacaagcgc
    11701 caactatcga tgaaatcttt aaactagaat caggagtgga agtatgagaa atatgtgggt
    11761 tgtaatcaag gaaacctatc ttcgacatgt cgagtcatgg agtttcttct ttatggtgat
    11821 ttcgccgttc ctctttttag gaatctctgt aggaattggg catctccaag gttcttctat
    11881 ggctaaaaat aataaagtgg cagtagtgac aacagtgcca tctgtagcag aaggactgaa
    11941 gaatgtaaat ggtgttaact tcgactataa agacgaagca agtgccaaag aagcaattaa
    12001 agaagaaaaa ttaaaaggtt atttgaccat tgatcaagaa gatagtgttc taaaggcagt
    12061 ttatcatggc gaaacatcgc ttgaaaatgg aattaaattt gaggttacag gtacactcaa
    12121 tgaactgcaa aatcagctta atcgttcaac tgcttccttg tctcaagagc aggaaaaacg
    12181 cttagagcag acaattcaat tcacagaaaa gattgatgaa gccaaggaaa ataaaaagtt
    12241 tattcaaaca attgcagcag gtgccttagg attctttctt tatatgattc tgattaccta
    12301 tgcgggtgta acagctcagg aagttgccag tgaaaaaggc accaaaatta tggaagtcgt
    12361 tttttctagc ataagggcaa gtcactattt ctatgcgcgg atgatggctc tgtttctagt
    12421 gattttaacg catattggga tctatgttgt aggtggtctg gctgccgttt tgctctttaa
    12481 agatttgcca ttcttggctc agtctggtat tttggatcac ttgggagatg ctatctcact
    12541 gaataccttg ctctttattt tgatcagtct tttcatgtac gtagtcttgg cagcattcct
    12601 aggatctatg gtttctcgtc ctgaggactc agggaaagcc ttgtcgcctt tgatgatttt
    12661 gattatgggt ggtttttttg gagtgacagc tctaggtgca gctggtgaca atctcctctt
    12721 gaagattggt tcttatattc cctttatttc gaccttcttt atgccgtttc gaacgattaa
    12781 tggctatgcg gggggagcag aagcatggat ttcacttgct attacagtga tttttgcggt
    12841 ggtagcaaca ggatttatcg gacgcatgta tgctagtctc gttcttcaaa cggatgattt
    12901 agggatttgg aaaaccttta aacgtgcctt atcttataaa tagaagagcc ttgcgaaagc
    12961 gaggttttta aaggtgaaaa gggtggaatc acgaacctaa tgatgtacac attatgtaaa
    13021 caggttgctt gaaaactcaa ttgtatagtg aactgaatct ggaataggat aatattactt
    13081 ctaaaacatt cttataaatt gatttgaatt ccaaaatccc atcgttccgt tcttatttca
    13141 ttttgcgata tagtgaaagg aaataagtca gctcacaaaa aacgcataat atcaaggatt
    13201 tttaacactt gatactatgc gttttctaat tttaaagact ttttgcctgg tctcttttgt
    13261 tctatgagta tgttacatat ctaggtaaaa attatttaaa gcaaattaac cttatttctc
    13321 aagatgtagt aggttccgag ataaaagata gctatgaata tgagttcttt aacaatggct
    13381 atacctgctc ccatatagaa atggtcattg agtcctacca gtgaattgac atagaaatta
    13441 gaactaagat tgaagaaaag ttcaataaat ccaatgacga tttggatacc aatgtaggct
    13501 gcaacagcga gtgctgtacg gtattcattg aaaagctgtc caatggaaat agccaggtag
    13561 atgcagagga ttcctgaaat ggtatttagt aggaaggata tacctgtaag aaagatctga
    13621 gggagatgtg tttctacaaa tgtaatcaca taagaaagag gaatccattc tggagctgtt
    13681 aaagccagaa taataacagc acttagagcc aatacagcgg tgctaatcaa tgaccagata
    13741 aaggcaccga ttagtttggc tgtgatgata tggtgttcag aaactggcaa ggtcaaagtc
    13801 agatagcctt gtcggtcgta gacactacct ttgaagcgtt taataatcaa gaaaatggtt
    13861 gaaatcgcaa gtgtaagcat caagccacca aagactgtag ctagaaaaag tagcatagta
    13921 gcctgacttt cttggtaagg tagattttta aagccttgtg tctgtattcc gatgagggca
    13981 gaaaggacta gcacggctgc gtagagggct aaataccact tgttaacatt tttaaattcg
    14041 tagtgaacta aattccaaaa cataaataat ctcctttgct taggccttaa attcctgacg
    14101 gaagagttgg tcaatggatt cacctgactc gtagcgaata tcatctacat ttccttgacg
    14161 gacgactttt ccgtctttta ggaagacaat ttcatccaag attggctcga tatcagaaat
    14221 caagtgggta gaaatcaaaa cggtagaagt tggtgagtag ttgttgataa tggtattgag
    14281 gatataagca cgggctgctg gatccacccc accaatgggt tcatccaaaa catagagacg
    14341 agcatcacgg ctcataacca aaatcagttg aaccttttct ttgtttcctt ttgatagttt
    14401 cttgagacga ctattttcat caatgcccag gtctgcaagt agatgatgag cgcgttcaag
    14461 attgaaatct ttatagaagg tcttgaagta ggttagggct tctttgacct tcatttgctc
    14521 attgagatag gtcgtatcag gcaaataagc tacaacggcc ttggttgctg ggcttgggtc
    14581 catgtcgttg atgaggacac gtccttgatc tggttgtaag aggccattaa ttagtttaat
    14641 cagggttgtt tttcctgagc cgtttggccc aagaaggccg acaatttttc cagctggaat
    14701 gtcaagagaa acattttcaa gggctggtgt tgctccatat gatttggata cattttcaaa
    14761 tactagtaat gacataggct taaactcctt taatataatc actgactacg cctggtagtt
    14821 cttctttttc atagccaaaa tggatcatgg aggaaacgaa gtgttccaat tcttcttctg
    14881 ataattgttt gcgtgactga gcgattagct ccttatcctt agtcacaaat cgtccagttg
    14941 ttcgcttgct gtagacaaat ccttctcgtt caaggtctga taaggctctt tggatggtat
    15001 tgggattgac accagcctcg ctagctagct ccctcacggt tggaagttgt tgattgggtt
    15061 ccagtgtatg ggaaacaatc tgaagcttga ttttctccat aatctgtaaa tagatgggtt
    15121 ttttgttgtc aaatgtccag gacatcttag tctcctttct cttatccttt tgtcttaact
    15181 aaataataca acaaaacaaa aaacttgtca agcaaaaaga agaattgttt tgggaatcgc
    15241 ttaaaaaatg gtataatgaa aagaaaacga taaggaggga aaggatgcgt tgtccaaaat
    15301 gtggggctac caagtcaagt gttatcgata gtcgccaagc agaagaaggg aacaccattc
    15361 gtagaagacg tgagtgcgac gaatgccaac accgttttac aacctacgaa cgagtagaag
    15421 aaagaacctt agtggttgtt aaaaaagatg gtacacggga acaattctcc agagataaaa
    15481 tctttaatgg gattatccgc tcagcccaga aacgtcctgt gtcaagtgat gaaatcaaca
    15541 tggtagtcaa tcgtatcgaa cagaaactcc gtggtcgaaa tgaaaatgaa attcaaagtg
    15601 aggacattgg ttcactcgtc atggaggagt tggctgaatt ggacgagatt acctatgtac
    15661 gttttgctag tgtctatcgt agttttaagg atgtcagtga gttagagagc ttgctccaac
    15721 aaatcaccca gtcctctaaa aagaaaaagg aaagataaat gaagccaatt gaccgttttt
    15781 cttatctaaa gaataatcgg gtgtcgcaag atacctcatc tctggtacag tgctacctcc
    15841 cgattatcgg tcaggaggca ctgagccttt atctttatac aatcagtttt tgggataatg
    15901 gtagaaagga atatcttttt tcaagtatcc tcaatcatct caactttggg atggatagac
    15961 tgataaaatc attgaaaatc ttatctgctt ttaatctctt gaccctctat caaaaggggg
    16021 atgtttatca gctagccctc catgctcctc tatctagtca agacttcttg gagcatcctg
    16081 tttatcgcag actcttagag aaaaagattg gcgactctgc tgtggaggat ttgaaagttg
    16141 aaagtgctga tggtgaagaa atacctgtct cactcaatca agtctttcca gaattggcag
    16201 aactaggcag tcaagaagac cttggtctca agaagaaagt ggccaatgat tttgacttgg
    16261 aacattttcg ccagctgatg gctcgagatg gacttcgttt tgcggatgag cagtccgatg
    16321 tcttaaacct ctttgctatt gccgaggaga agaaatggac ttggtttgaa acctatcaat
    16381 tggccaagtc aacagctgtt tctcaggtta tttcaaccaa acgcatgcgt gaaaaaattg
    16441 ctcaaaaacc agtttcctct gactttaatc ctaaggaagc aaccattatc aaggaagcta
    16501 aaagtaagac tgccctgcag ttcttagcag aaatcaagca aacacgcaag gggaccatta
    16561 cccaaacaga aagagaactt ttgcaacaga tggctggctt gggcttgctg gacgaagtca
    16621 tcaatatcat tctcttgttg acctttaata aggtggattc ggcaaacatc aatgagaaat
    16681 atgccatgaa ggtggctaat gactatgcct atcaaaagat acattcagca gaagaggccg
    16741 ttttacgaat tcgtgagcga gggcagaaaa caaaaacaca aaaacagaat cagactgccc
    16801 cagcaaaaac caatattcct aaatggagta acccagatta taagaatgaa accagcgagg
    16861 aaactcgtct ggaactagaa cgtaagaaac aagaactatt agctcgatta gaaaaaggag
    16921 gagattagat ggaaagtgtc ggagacgtac tcaaacgtca acccagccgt ttttattatc
    16981 aagatttggt ccagaaaatc atgaaggacc ctgatgttgc ggcttttatc cagcaagaat
    17041 ccttgactcc agaggaatta aatcgcagta tctccaagtt taatcagtac atcaccgagc
    17101 gtgacaagtt tctccgtggg gatacggatt atattgccaa aggctacaag ccgattttgg
    17161 ttaagaatca tggctatgca gatgtttcat atgaagaaac tcctgagcta atcgcggctg
    17221 aaaaagaagc ggctattaag aatcgtctca agctaatcaa tctaccagcc agtctcaaaa
    17281 aagctagttt ggctcaagtt gatttggatg atttgggacg cttgccagtt tttgaaaaac
    17341 tattagcctt tgtggagcaa tatccagcta ttcgaaaagg tctttactta tatggagact
    17401 ttggtgtggg taaaagtttc atggtggcgg ccttagctca tgatttatca gaaaaacgtg
    17461 gtgtttcatc aactctcctc cactatccta gctttgtcat tgatgtcaaa aatgctatca
    17521 gtgatggcaa tgttaagacc ttggtggatg agattaagct gtctgaggtc ctgattttag
    17581 atgatattgg cgccgagcaa tcaaccgctt gggtgcgtga cgaaatcctg caggtcattc
    17641 tccaatatcg gatgcaggaa aatttaccga cctttttcac atccaacttc aactttgaag
    17701 atttggagaa gcattttgcg aaagtgaaac atggaaatga cgaaacctgg gaagccagac
    17761 gggtcatgga acgcatccgt tatttggctg aggagactcg tttagaagga gtaaaccgtc
    17821 gatgacagaa accattaaat tgatgaaggc tcatacttca gtgcgcaggt ttaaagagca
    17881 agaaattccc caagtagact taaatgagat tttgacagca gcccagatgg catcatcttg
    17941 gaagaatttc caatcctact ctgtgattgt ggtacgaagt caagagaaga aagatgcctt
    18001 gtatgaattg gtacctcaag aagccattcg ccagtctgct gttttccttc tctttgtcgg
    18061 agatttgaac cgagcagaaa agggagcccg acttcatacc gacaccttcc aaccccaagg
    18121 tgtggaaggt ctcttgatta gttcggtcga tgcagctctt gctggacaaa acgccttgtt
    18181 ggcagctgaa agcttgggct atggtggtgt gattatcggt ttggttcgat acaagtctga
    18241 agaagtggca gagctcttta acctacctga ctacacctat tctgtctttg ggatggcact
    18301 gggtgtgcca aatcaacatc atgatatgaa accgagactg ccactagaga atgttgtctt
    18361 tgaggaagaa taccaagaac agtcaactga ggcaatccaa gcttatgacc gtgttcaggc
    18421 tgactatgct ggggcgcgtg cgaccacaag ctggagtcag cgcctagcag aacagtttgg
    18481 tcaagctgaa ccaagctcaa ctagaaaaaa tcttgaacag aagaaattat tgtagaaagt
    18541 gagaaattat ggccctacca actattgcca ttgtaggacg tcccaatgtt gggaaatcaa
    18601 ccctatttaa tcggatcgct ggtgagcgaa tctccattgt agaagatgtc gaaggagtga
    18661 cacgtgaccg tatttatgca acgggtgagt ggctcaatcg ttcttttagc atgattgata
    18721 caggaggaat tgatgatgtc gatgctcctt tcatggaaca aatcaagcac caggcagaaa
    18781 ttgccatgga agaagcagat gttatcgtct ttgtcgtgtc cggtaaggaa ggaattaccg
    18841 atgcagacga atacgtagcc cgtaagcttt ataagaccca caaaccagtt atcctcgcag
    18901 tcaacaaggt ggacaaccct gagatgagaa atgatatcta tgatttctat gctctcggtt
    18961 tgggtgaacc attgcctatc tcatctgtcc atggaatcgg tacaggggat gtgctagatg
    19021 cgatcgtaga aaatcttcca aatgaatatg aggaagaaaa tccagatgtc attaagttta
    19081 gcttgattgg tcgtcctaac gttggaaaat caagcttgat caatgctatc ttgggagaag
    19141 accgtgttat tgctagtcct gttgctggaa caactcgtga tgccattgat acccacttta
    19201 cagatacaga tggtcaagag tttaccatga ttgatacggc tggtatgcgt aagtctggta
    19261 aggtttatga aaatactgag aaatactctg ttatgcgtgc catgcgtgct attgaccgtt
    19321 cagatgtggt cttgatggtc atcaatgcgg aagaaggcat tcgtgagtac gacaagcgta
    19381 tcgcaggatt tgcccatgaa gctggtaaag ggatgattat cgtggtcaac aagtgggata
    19441 cgcttgaaaa agataaccac actatgaaaa actgggaaga agatatccgt gagcagttcc
    19501 aatacctgcc ttacgcaccg attatctttg tatcagcttt aaccaagcaa cgtctccaca
    19561 aacttcctga gatgattaag caaatcagcg aaagtcaaaa tacacgtatt ccatcagctg
    19621 tcttgaacga tgtcatcatg gatgccattg ccatcaaccc aacaccgaca gacaaaggaa
    19681 aacgtctcaa gattttctat gcgacccaag tggcaaccaa accaccaacc tttgtcatct
    19741 ttgtcaatga agaagaactc atgcactttt cttacctgcg tttcttggaa aatcaaatcc
    19801 gcaaggcctt tgtttttgag ggaacaccga ttcatctcat cgcaagaaaa cgcaaataaa
    19861 aaagtagaat ctggaatgac agttccggat ttttttgata taataaaata atagaaaacg
    19921 ctatcaaaag aaggagggtg atgtcggtga tggaacattt gtttaaattc ttacttttag
    19981 caccgtactt ttattttgat aactggattg agaaggccaa cagaaatagt aagttttttc
    20041 caatttttta ttatttttac tggttttaca tccccttcta ttctcttttt agccttgctt
    20101 ggacagttgt ttcagttctg tttttcaata ccgtcttgag aaatgtgaca gatatcaagt
    20161 tatggggcat ttggtttctt tttattctgc tagctattgg tatgaattgg ttaacttatt
    20221 cctgtttcaa agaaatgttt cgcttgagac aggaactagg gaagtctaag ggtggaaggc
    20281 attgatttat atcggtttct atttgtagga ggtggcttat ggtacctcta aaatcattta
    20341 ttacacgcta ttccaaggtt tatattggta ataacattta atagaacgaa actaaagaaa
    20401 acaatttaaa agaggaggtt tgtgatggaa tacttgttta aaatgataat cttattaccc
    20461 tgtttttact ttttcagctg gattgaaaag gcaaacagag atagtaagat tttcccaatt
    20521 ttttactatt tttactggat ttacatcacc ctctatgctc tttttagcct tgcttggaca
    20581 gttttttcag ttctgttttt caatatcgtc ttgagaaatt tgacagatat caagttatgg
    20641 ggtatttggc tattattact acttatcgct tttgctagtg actggttaga ctatatctgt
    20701 ttcaaaaaaa tgcttgactt gagacgggaa ctagggaagt ctaaaggcgg aaggcattga
    20761 tttatactct tcgaaaatct cttcaaacca cgtcaacgtc gccttggatt atatatgtaa
    20821 ctgacttcgt cagtcttatc tacaacctca aagcagtgct ttgagcaact tgcggctagt
    20881 ttcctagttt gctctttgat tttcattgag tattatatta ctttctattt gtaggaggtg
    20941 gcttatgaag attcctctct taactttggc aaggcataaa tttgtttatg tcttgcttac
    21001 tttgcttttt cttgctttgg tttatcgtga tgttttgatg acttatttct tttttgatat
    21061 tcatgcgccc gatctagcta aattcgatgg acaagcaatt aaaaatgact tattaaaatc
    21121 agcattagat tttcgtattc tccagttcaa tctaggtttt tatcaatcat ttattattcc
    21181 aatcatcatt gttttgctag gttttcaata tattgagctg aaaaataaag ttttacgatt
    21241 gagtattgga agagaagtga gctatcaagg gttaaaaaga aagttgactt tgcaagttgc
    21301 aagtatccct tgtttgatat atttagtgac tgtgctgata attgcaatta taacctattt
    21361 ctttgggact ttttctcctc ttgaatggaa ttctctattt tctgatggga gtggtttaca
    21421 aagactccta gatggagaga taaaaagcta tttgttcttt acttgtgtcc tactaatcgg
    21481 tattttcatc aatgcaatct attttttaca aatagttgat tatgtgggga atgtgactcg
    21541 ttcggcaatc acctatttga tgtttctttg gcttggttct atgctgctct atagtgcctt
    21601 gccttactat atggttccta tgacgagttt gatgcaagct agctatgggg atgtaagttt
    21661 gatgaaactc tttactcctt atatccttta tgttgtccct tatatggtac ttaaaaaata
    21721 tgaagataat gtttaagaat tttaacaata tttggctaaa gagaaagatc gttttactac
    21781 ttcgtatagt tctgatgatg attttgataa actatctatt gtcaacagcg gttcaaaagc
    21841 aggatgctgt tatctttttc aagagagaat tgatttcaat tttttcctat aatgactatt
    21901 ctgaagcgaa tttagaaatc cccaaactct tgttaaacct ttcgcttttc atggtaggat
    21961 ggctctctgt cattttactt gaaagtgatt tggcagacca ttaccatcac ttgattcgct
    22021 atcaatcaag ctcctttttc gattatacaa ggaaacgatt ggttgtcatt tctaaatttt
    22081 ttactcaaga tttgtttgtc tggtttcttg gtttacttcc gctaggaatt catttcaaaa
    22141 cagtcgcact tttcttttta cttgctcagt taatgatgtt gtacttacta ctgtcttatc
    22201 tgatagcact gattagtgcg ggcgctggtt tttctttttt tctctatttt ttggcatttg
    22261 tgggacaaga atggatgatg gatcatattg taacagtgta tttagtactc ttaagcttat
    22321 tagttatgtt gattgttagt cgcttggaag agaaatttaa gaaaggataa acgatgagac
    22381 ttgaaattat aaatggacag aaaatttatg ggaaaagacc tattttaaat cagttgaatt
    22441 tggtgtttca atcaggaaaa atttatggac ttaaaggtga taatggatct ggcaagacgg
    22501 ttcttttaaa gatacttgct ggttatatta agcttgacaa aggaaaagtt cttcaagatg
    22561 gtaaagttta cggggtaaaa aatcattata ttcaggatgc aggaatttta attgaaaaag
    22621 tcgagttttt atctcattta tccctgagag aaaatttgga actgttaagg tatttttcat
    22681 ctaaagttac ggaaaaaaga attgcctatt ggattcaata ctatgattta caggaatttg
    22741 aagacgttga ataccgtcat ttatccttgg gaacaaagca aaaaatggcc ttgattcaag
    22801 cctttatttc ctctccttct atactctttc tcgatgaacc tatgaatgct ttggatgaga
    22861 agagtgtgag gttaaccaaa caggtcattt tatcttacct gaaaaaagaa aatggtctgg
    22921 ttatcctgac gtcgcacata tcggaagata tttcagacct ttgtacagat gtattagttg
    22981 tcgaaaatgg acatatacaa tatgtaaagg atatacaatc ctaggagatg gcttatggca
    23041 catctaaaat catttattac acgctattcc aaggtttata ttggtttagt tctgctgatc
    23101 tggctgtctt tcttctttat cccttgggat agagcgattc tgggggtaag gattgacatc
    23161 ttcatcatgc agaaaatctt gctagctttt ggaattctgt ccattctcat ggccttgctg
    23221 tccaagaaag tcagtctctt tgtttttgga ctgatttgct gtctttctct ttggattaac
    23281 ttatttatca catttgccat tttgccgatt tttggcaatt aaacagtcat aaaagtcgga
    23341 gaggttagct tgaaaactaa cctctttttc cttttcaaaa tggggattct tccttgaaaa
    23401 taatcagtaa ttgtgctaaa attaaaggaa cattctaaaa tattcggaat ttaaagtaag
    23461 gaaaaacatg gctaatattt taaaaacaat tatcgaaaat gataaaggag aaatccgtcg
    23521 tctggaaaag atggctgaca aggttttcaa atacgaagac caaatggcta ctttgactga
    23581 cgaccaacta aaagcaaaaa cagttgaatt taaggaacgt tatcaaaatg gagaatcact
    23641 ggattcattg ctttacgaag catttgcggt tgtccgtgaa ggtgccaaac gtgtcctagg
    23701 tctcttccct tataaggttc aggtcatggg ggggattgtt cttcaccatg gtgacgtgcc
    23761 agagatgcgt acaggggaag ggaaaacctt gactgcgacc atgccggtat acctcaatgc
    23821 cctttcaggt aaaggggttc acgtagttac ggttaatgaa tacctgtcag aacgtgacgc
    23881 gactgagatg ggtgaattgt actcttggct tggtttgtca gtagggatta acttggctac
    23941 caaatctcca atggagaaaa aagaagccta tgagtgtgat attacttact caactaactc
    24001 agaaatcgga tttgactacc ttcgtgacaa catggtcgtt cgcgccgaaa acatggtaca
    24061 acgtccgctt aactatgcct tggtcgatga ggttgactct atcttgattg acgaggctcg
    24121 tacacctttg attgtatcag gtgccaatgc ggttgaaacc agtcagttgt atcacatggc
    24181 agaccactat gtaaaatctt tgaacaaaga tgactacatc atcgatgtgc agtctaagac
    24241 tattggtttg tctgattcag ggattgacag ggctgaaagc tacttcaaac ttgaaaacct
    24301 ctatgacatc gaaaacgtgg ctttgactca ctttatcgat aacgcccttc gtgccaacta
    24361 catcatgctt ctcgatattg actatgtggt gagcgaagag caagaaatct tgattgtcga
    24421 ccaatttaca ggtcgtacca tggaaggtcg tcgttattct gatggattgc accaagctat
    24481 tgaagccaaa gaaggtgtgc caatccagga tgaaaccaag acatctgcct caatcacgta
    24541 ccaaaacctt ttccgtatgt acaagaaatt gtctggtatg acgggtacag gtaagactga
    24601 ggaagaagaa ttccgtgaaa tctacaacat tcgtgttatt ccaatcccaa caaaccgtcc
    24661 tgttcaacgt attgaccact cagaccttct ttatgcaagt atcgaatcta agtttaaagc
    24721 ggttgtcgaa gacgttaagg ctcgttacca aaagggtcaa cctgtcttgg ttggtacagt
    24781 agcggttgaa actagtgact acatttctaa gaaattggtt gcagctggtg ttcctcacga
    24841 agtcttgaat gccaaaaacc actatagaga agcccaaatc atcatgaatg ctggtcaacg
    24901 tggtgccgtt accatcgcaa ccaacatggc gggtcgtggt accgacatca agcttggtga
    24961 aggtgttcgt gaacttggag gactttgtgt tattggtaca gaacgtcatg aaagtcgtcg
    25021 tatcgataac cagcttcgtg gacgttcagg tcgtcaagga gatccaggtg agtcacaatt
    25081 ctacctatct cttgaagatg atttgatgaa acgttttggt tctgaacgct tgaagggaat
    25141 ctttgaacgt ttgaacatgt ctgaagaggc gattgagtct cgcatgttga cgcgtcaggt
    25201 tgaagcagct cagaaacgtg tcgaaggaaa taactacgat acccgtaaac aagtccttca
    25261 atacgatgat gtcatgcgtg aacaacgtga gattatctat gctcaacgtt acgatgtcat
    25321 cactgcagat cgtgacttgg cacctgaaat tcaggctatg atcaaacgca cgattgaacg
    25381 tgtcgttgat ggtcatgcgc gtgccaaaca agatgaaaaa ctagaggcaa ttttgaactt
    25441 tgctaagtac aacttgcttc ctgaagattc tattacgatg gaagacttgt caggcttgtc
    25501 tgataaggcc atcaaggaag agcttttcca acgtgccttg aaggtttacg atagtcaggt
    25561 ttcaaaacta cgcgatgaag aagcagttaa agaattccaa aaagttttga ttctacgagt
    25621 ggtggataac aagtggacag atcatatcga tgcccttgat caattgcgta acgcggttgg
    25681 acttcgtggc tatgctcaga acaaccctgt tgttgagtat caggcagaag gtttccgtat
    25741 gtttaatgat atgattggtt cgattgagtt tgatgtgaca cgcttgatga tgaaagcaca
    25801 aattcatgaa caagaaagac cacaggcaga acgtcatatc agtacaacag cgactcgcaa
    25861 tatcgctgct caccaagcaa gtatgccaga agatttggat ttgagccaga ttggacgcaa
    25921 tgaactttgc ccatgtggtt ctggtaagaa atttaaaaac tgtcacggta aaagacaata
    25981 aaatgagata gtttagaggc ggatatcttg tgaaaagtaa atttttactg ggtatccgtt
    26041 tgctttataa ggagatgagt tatggtattt acagcaaaaa gctctaaaat aaatatagaa
    26101 gaagttcgtg ccttgtcaaa attagaaggt caggctttgg agaggaaatc acagcgcgat
    26161 caagagctag aagccattat acgtggagaa gatcagcgaa ttctcttggt aatcgggcca
    26221 tgctcatctg acaacgaaga agctgtcctt gaatacgcta agcgtttggc agtcctacaa
    26281 gaagaagtgg cagatcgtat ctttatggtt atgcgtgttt atactgccaa accccgtacc
    26341 aacggagatg gctataaggg cttgattcac cagcctaacg cgacagaagc gcctagtctt
    26401 atcaatggaa tcaaagccgt tcgccatctt cactatcgtg tcatcacaga aacagggatg
    26461 acaactgctg atgaaatgct ttatcctgaa aaccttccgc ttgtagatga tttgatttct
    26521 tacatggcag ttggtgcccg ttcagttgaa gaccagcaac accgctttgt ggcaagtggg
    26581 gcaggatttt ctactggttt taaaaatcca acctctggaa atctcaatgt catgtttaat
    26641 gggatttatg ctgctcaaaa caaacaaagt ttccttttct taggaaaaga agtagaaaca
    26701 actgggaacc cgctttcaca cgctattctt cgtggtgctc ttaatgagta tggaaaaaat
    26761 attcccaact actattatga caatttaatt gataccattg cccaatatga gaaaatgggc
    26821 ttggaaaatc cttttatcat cattgatacc aatcatgaca attctggtaa gcagtatatt
    26881 gaacagatcc gaattgtccg ccagaccttg attaaccgtg cttggaatga aaaaattaag
    26941 cagttcgttc gtggttttat gattgagtct tatctggaag atggtcgaca aaatgagcca
    27001 gaagtatttg gcaagtctat cacagaccct tgcctgggtt gggataacac agaagctctt
    27061 gtcagagaaa tttacaaaac gttaggagaa taagatggca tttattgaaa aaggtcaaga
    27121 aatcgatatg gaagtcatca aggctgaaac ccaattgtct gcggaagcct tgagactcaa
    27181 ggaaagccgt gacagggaat tggcagatat tatttcaggg gaagatgacc gtattctctt
    27241 ggtgattggt ccttgctctt ctgataatga agaggcggtc ttggaatatg ctcgccgttt
    27301 atccgccttg caaaagaagg tagcggacaa gattttcatg gtcatgcgcg tgtatactgc
    27361 taagcctcgt accaatggag acggctataa aggattggtt caccagccag atacttctaa
    27421 ggctccaagc ctgattaacg gtttgcaggc tgtgcgccag ttgcattacc gcgtgattac
    27481 agagactggt ttgacaacgg cagatgagat gctttatccg tcaaatctga tcttggtgga
    27541 tgacttggtc agctaccatg ccgttggagc tcgttctgtg gaagaccaag agcaccgctt
    27601 tgtggcttct gggattgatg caccagtagg gatgaaaaat ccaacctcag gaaatttggg
    27661 tgttatgttt aacgccatct atgctgctca aaacaagcaa accttccttt atcatgggca
    27721 ggaagttgag acatcaggta atcctttggc ccatgttatc ctccgtggag cagtcaacga
    27781 gtatggcaat tatatgccga attactacta tgaaaaccta ctccaagcca ttgaacgcta
    27841 tgaaaccatg ggacttgaaa atccttttat cctcattgac accaaccatg ataactcagg
    27901 caagcaatat atggagcaga ttcgaattgt tcgccagacc ttgcagaatc gtgattggaa
    27961 tgagaaaatt aaaaagacgg ttcgaggatt tatgattgaa tcttacctag cagatggtcg
    28021 tcaaaaccaa ccagagatct ttggttgctc tattactgac ccttgcctag gttgggaaaa
    28081 tacagaggcc ttggtagaag agatttatgt taccttgaca aaataagtga aaaggatgga
    28141 gttggggaat ctcaactcct tttgatgaga atgatagttg gacacggaat tgacatcgaa
    28201 gaattggctt cgatagaaag cgcagttaca cgacatgaag gatttgctaa gcgtgtactg
    28261 accgctcagg aaatggagcg cttcaccagt ctcaaaggac gcaggcaaat agaatattta
    28321 gctggtcgct ggtcggctaa ggaggccttt tccaaggcta tgggaacggg cattagcaag
    28381 ctcggttttc aggatttgga agtattgaac aatgaacgtg gggcgcctta ttttagtcag
    28441 gcaccatttt caggaaagat ttggctgtct atcagccaca ccgatcagtt tgtgacagcc
    28501 agtgtcattt tggaggaaaa tcatgaaagc tagtccacat agaccaacca aggctctgat
    28561 tcatctggga gctattcgac aaaatattca gcaaatgggg gctcatatcc ctcaaggaac
    28621 gctcaagttg gctgtggtta aggccaatgc ttatggtcat ggagctgttg ccgttgccaa
    28681 ggcaattcaa gatgatgttg atggcttttg cgtttccaat atcgatgaag ccattgaact
    28741 cagacaagct ggactcagca agccaatcct cattttagga gtttctgaaa tcgaagctgt
    28801 tgctctagct aaagaatatg acttcacctt gacagtggct ggactggagt ggattcaagc
    28861 actcttagat aaggaagtgg acctaactgg attgacagtc cacctcaaga ttgattcagg
    28921 gatgggacgg attggtttta gagaggcaag tgaggttgag caggctcaag atttgctcca
    28981 acaacacggt gttcgtgttg aaggaatctt tacccacttt gctactgctg atgaggaatc
    29041 agatgactat tttaatgccc agttagaacg gtttaaaact attttagcta gtatgaagga
    29101 agttccagag ctggttcatg ctagcaattc tgcaacgact ctttggcatg tagagactat
    29161 tttcaatgcg gttcgtatgg gagatgccat gtatggtctc aatccaagtg gagcggtctt
    29221 ggctttgcct tatgatttga taccggcctt gaccttggag tctgctctgg ttcatatcaa
    29281 gacagttcca gctggtgctt gcatgggcta tggagcaact tatcaaacgg atagcgagca
    29341 agtcatcgcg accgtgccaa tcgggtatgc agatggatgg acaagagaca tgcaaaattt
    29401 ctctgtcttg gtagatggcc aagcttgccc aattgtcggc agggtttcga tggaccaaat
    29461 cactattcga ttgcctaagc cttacccact cggaaccaag gtaaccttga ttggctccaa
    29521 tggggataag gaaatcactg caactcaggt agcgacctac cgcgtaacca ttaactatga
    29581 ggtggtttgc ctcctcagcg accgtattcc gagagaatat tactagaaaa gaaaggagtg
    29641 gagcatgaat ctacatcaac ccttgcatgt cttgcctggt gtgggaccaa agtcagcaga
    29701 aaaatacgcc aaactaggaa ttgaaaactt gcaagatctc ttgctctact ttcctttccg
    29761 ttatgaagac ttcaaaatca agcaggtgct ggagctggaa gacggtgaga aggcagttct
    29821 ttctggtcag gtagtgactc ctgctagtgt ccagtattat ggtttcaagc gcaatcgcct
    29881 gcgttttagt ctcaagcagg gagaggtcgt ttttgcggtg aatttcttta accagcccta
    29941 tctggctgat aaaatagagt tgggagcaac ccttgctgtc tttggaaaat gggaccgcgc
    30001 taaggctagt ctgactggga tgaaggttct ggctcaggta gaagatgacc tccagcctgt
    30061 ctatcgtctg gctcagggaa tcagtcaggc cagtctggtt aaggtcatca agacggcttt
    30121 tgatcaggga ctggacctct tgatagaaga aaatctgccc cagtctttac tagacaaata
    30181 caaactcatg tcccgttgtc aggcagtccg agctatgcat tttccaaagg atttggcaga
    30241 atacaagcag gctcttcgcc gtatcaagtt tgaggaactc ttttatttcc aaatgcagct
    30301 gcagatgctc aagtctgaaa atagagttca gggaagtggt ctggttctga attggtctca
    30361 ggaaaaagtg acagcagtta aagtaagtct tccttttgcc ctgacccaag ctcaggaaaa
    30421 gagtttgcag gaaattttaa ctgatatgaa gtccgaccac cacatgaatc gtctcctaca
    30481 aggggatgtg gggagtggaa aaacggtagt cgctggcttg gccatgtttg cggcagtgac
    30541 agcaggttat caggctgccc taatggtacc aacagaaatc ctcgcagagc aacactttga
    30601 gagtttacag aacctttttc ccaatttgaa actggctctc ttgacaggtt ccttgaaagc
    30661 tgcagaaaag agagaggtct tggagaccat tgccaagggt gaggctgatt tgattatcgg
    30721 aactcacgct ctgatacaag atggggtgga gtatgctcgt cttggtttga ttattatcga
    30781 tgagcagcac cgttttggtg tagggcaaag gcgtatttta cgggaaaaag gtgacaatcc
    30841 agatgtcctc atgatgacgg cgactcccat tccacggacg cttgccatca cagcctttgg
    30901 agatatggat gtttccatta tcgaccagat gccagcaggt cggaagccta ttgtgacgcg
    30961 ctggatcaaa catgagcaac tacctcaggt cttgacttgg ttagaggggg aaattcaaaa
    31021 aggttcccaa gcctatgtca tctctccttt gattgaagaa tcagaagctc tagatttgaa
    31081 aaatgccatt gccttatcag aggagttgac gactcatttt gcaggcaagg cagaggtggc
    31141 tcttctacat ggtaggatga agagtgacga aaaagaccag atcatgcagg atttcaagga
    31201 gagaaagacg gatattctgg tttcgacgac ggttattgag gttggggtca acgttcccaa
    31261 tgcgactgtc atgattatca tggatgccga tcgcttcggt ctcagtcaac ttcaccagct
    31321 tagaggtcgt gtcggtcggg gggacaagca gtcctacgct gttctcgttg ccaatcccaa
    31381 gacggattct gggaaagacc gcatgcgtat catgacagaa acgaccaatg gatttgtcct
    31441 tgcggaggaa gatttgaaaa tgcgtggttc tggtgagatt tttggaacca gacagtcagg
    31501 acttccagag ttccaagtgg ctgatattat cgaagatttt ccgattttag aagaagcaag
    31561 aaaggttgct agctacatta gttctataga agcttggcaa gaagatccag agtggcgcat
    31621 gattgccctt catctggaaa agaaagaaca tctggattaa gctttctcta aggaaaactt
    31681 aagctagttt ttaggtttgg ctcttatact aaagtcatca aaaagaaacg aggactctca
    31741 tatgacagta acgattaaag taaattacca aaccactttc caaaagaagg aagcaaaaaa
    31801 ctagtataaa cagaagagag agcgaaatgc tctttttcgt ttctaaaact actttcagcc
    31861 catcatccta aaagtaaaga atctaaattc actttctatt tatccttctt tcttgcattg
    31921 attacataga tatgctacag ttgtggtaac gattacaaaa taaaaggagc atgctatgaa
    31981 aaatccagct ttgctagaag aaattaagac ctatagagga agggatgagg ttccggaaga
    32041 ctttgatgct ttctgggatg gggaagtgaa aaatgtttcc acgcttccat cctaccactt
    32101 ggaggaaaga gatttccaca ttcctcaagt caagtgctat gagttaacat ttgaaggaag
    32161 caaggaagga aaggtctatg cacgcattgt tcttccaaag agtgaggaga aggttccact
    32221 aatcttccat tttcatggtt atatgggacg tggctgggac tgggccgaca tgctgggctt
    32281 caccgtagct ggttacggtg ttgtttccat ggatgtgcgg ggccagtcag gttactcaca
    32341 agacggcttg cgttctcctt taggaaatac cgtgaagggg catattatcc gtggtgctat
    32401 ggaaggtcgg gaccacctct tttataagga tgtttatctg gatatttacc agttggtcga
    32461 aattgttgct agtctgtctc aggttgatga gaagcgtctt tctagctatg gtgcctcaca
    32521 aggaggggct ctagctctag ttgcagcagc gctcaatcct cgaattcaga aaacagttgc
    32581 catttatccc ttcttgtcag acttcagacg ggtgattgag attggtaata ctagcgaggc
    32641 ttacgacgaa cttttccgtt atttcaagtt tcacgacccc ttccatgaaa cagaggagga
    32701 aatcatggcg acccttgcct atatcgatgt caaaaatctt gcccatcgta tccaaggtga
    32761 ggttaagatg attacgggct tggacgacga tgtttgctat cccattaccc agtttgcgat
    32821 ttataatcgt ctgacctgcg ataaaaccta tcgcatcatg cctgagtatg ctcacgaagc
    32881 catgaatgta tttgtcaatg accaagtcta caactggctc tgtggaagtg agattccttt
    32941 taaatatcta aaataaggag tcgactctaa gcacaaaatc ttaaaaatta caaacacgca
    33001 tagtatcagg ggattaaaaa aacttgatac tatgcgtttt atcatggaaa tatagtaaaa
    33061 tgaaacaaga ataggacaaa acgatcagga cagtcaaatc gatttctaac aatgttttag
    33121 aagcaaatgt gtactattct agtttcaatc tattatattt atagaatttt ttgttgctag
    33181 atttgtcaaa ttgcttaaaa taattttttt cagaaagcaa aagccgatac ctatcgagta
    33241 gggtagttct tgctatcgtc aggcttgtct gtaggtgtta atacttttca aaaatctctt
    33301 taaaccacgt cagcttcgcc ttgccgtata tatgttactg acttcgtcag ttctatctgc
    33361 cacctcaaaa cggtgttttg agctgacttt gtcagtctta tctacaacct caaaacagtg
    33421 ttttgagcat catgcggcta gcttcttagt ttgctctttg attttcattg agtataaaaa
    33481 cagatgagtt tctgttttct ttttatggac tataaatgtt cagctgaaac tactttcaag
    33541 gacattatta tataaaagaa ttttttgaaa ctaaaatcta ctatattaca ctatattgaa
    33601 agcgttttaa aaatgaggta taataaattt actaacgctt ataaaaagtg atagaatcta
    33661 tctttatgta tatttaaaga tagattgctg taaaaatagt agtagctatg cgaaataaca
    33721 gatagagaga agggattgaa gcttagaaaa ggggaataat atgatattta aggcattcaa
    33781 gacaaaaaag cagagaaaaa gacaagttga actacttttg acagtttttt tcgacagttt
    33841 tctgattgat ttatttcttc acttatttgg gattgtcccc tttaagctgg ataagattct
    33901 gattgtgagc ttgattatat ttcccattat ttctacaagt atttatgctt atgaaaagct
    33961 atttgaaaaa gtgttcgata aggattgagc aggaagtatg gtataaatag cataagctga
    34021 tgtccatcat ttgcttataa agagatattt tagtttaatt gcagcggtgt cctggtagat
    34081 aaactagatt ggcaggagtc tgattggaga aaggagaggg gaaaattggc accaatttga
    34141 gatagtttgt ttagttcatt tttgtcattt aaatgaactg tagtaaaaga aagttaataa
    34201 aagacaaact aagtgcattt tctggagtaa atgtcttatt tcagaaatcg ggatatagat
    34261 atagagagga tcagtatgaa tcggagtgtt caagaacgta agtgtcgtta tagcattagg
    34321 aaactatcgg taggagcggt ttctatgatt gtaggagcag tggtatttgg aacgtctcct
    34381 gttttagctc aagaaggggc aagtgagcaa cctctggcaa atgaaactca actttcgggg
    34441 gagagctcaa ccctaactga tacagaaaag agccagcctt cttcagagac tgaactttct
    34501 ggcaataagc aagaacaaga aaggaaagat aagcaagaag aaaaaattcc aagagattac
    34561 tatgcacgag atttggaaaa tgtcgaaaca gtgatagaaa aagaagatgt tgaaaccaat
    34621 gcttcaaatg gtcagagagt tgatttatca agtgaactag ataaactaaa gaaacttgaa
    34681 aacgcaacag ttcacatgga gtttaagcca gatgccaagg ctccagcatt ctataatctc
    34741 ttttctgtgt caagtgctac taaaaaagat gagtacttca ctatggcagt ttacaataat
    34801 actgctactc tagaggggcg tggttcggat gggaaacagt tttacaataa ttacaacgat
    34861 gcacccttaa aagttaaacc aggtcagtgg aattctgtga ctttcacagt tgaaaaaccg
    34921 acagcagaat tgccaaatgg tcgagttcgc ctgtatgtta atggtgtttt atctcgaaca
    34981 agtctgaaat ctggcaattt cattaaagat atgccagatg taacgcatgt gcaaatcgga
    35041 gcaaccaagc gtgccaacaa tacggtttgg gggtcaaatc tacagattcg gaatctcact
    35101 gtgtataatc gtgctttaac accagaagag gtacaaaaac gtagtcaact ttttaaacgc
    35161 tcagatttag aaaaaaaact acctgaagga gcggctttaa cagagaaaac ggacatattc
    35221 gaaagcgggc gtaacggtaa accaaataaa gatggaatca agagttatcg tattccagca
    35281 cttctcaaga cagataaagg aactttgatc gcaggtgcag atgaacgccg tctccattcg
    35341 agtgactggg gtgatatcgg tatggtcatc agacgtagtg aagataatgg taaaacttgg
    35401 ggtaaccgag taaccattac caacttacgt gacaatccaa aagcttctga cccatcgatc
    35461 ggttcaccag tgaatatcga tatggtgttg gttcaagatc ctgaaaccaa acgaatcttt
    35521 tctatctatg acatgttccc agaagggaag ggaatctttg gaatgtcttc acaaaaagaa
    35581 gaagcctaca aaaaaatcga tggaaaaacc tatcaaatcc tctatcgtga aggagaaaag
    35641 ggagcttata ccattcgaga aaatggtact gtctatacac cagatggtaa ggcgacagac
    35701 tatcgcgttg ttgtagatcc tgttaaacca gcctatagcg acaagggtga tctatacaag
    35761 ggtaaccaat tactaggcaa tatctacttc acaacaaaca aaacttctcc atttagaatt
    35821 gccaaggata gctatctatg gatgtcctac agtgatgacg acgggaagac atggtcagcg
    35881 cctcaagata ttactccgat ggtcaaagcc gattggatga aattcttggg tgtaggtcct
    35941 ggaacaggaa ttgtacttcg gaatgggcct cacaagggac ggattttgat accggtttat
    36001 acgactaata atgtatctca cttaaatggc tcgcaatctt ctcgtgtcat ctattcagat
    36061 gatcatggaa aaacttggca tgctggagaa gcggtcaacg ataaccgtca ggtagacggt
    36121 caaaagatcc actcttctac gatgaacaat agacgtgcgc aaaatacaga atcaacggtg
    36181 gtacaactaa acaatggaga tgttaaactc tttatgcgtg gtttgactgg agatcttcag
    36241 gttgctacaa gtaaagacgg aggagtgact tgggagaagg atatcaaacg ttatccacag
    36301 gttaaagatg tctatgttca aatgtctgct atccatacga tgcataatgg aaaagaatac
    36361 attatcctca gcaatgcagg tggaccgaat cgtgaaaatg ggatggtcca cttggcacgt
    36421 gtcgaagaaa atggtgagtt gacttggctc aaacacaatc caattcaaaa aggagagttt
    36481 gcctataatt cgctccaaga attaggaaat ggggagtatg gcatcttgta tgaacatact
    36541 gaaaaaggac aaaatgccta taccctatca tttagaaaat ttaattggga atttttgagc
    36601 aaagatctga tttctcctac cgaagcgaaa gtgaagcgaa ctagagagat gggcaaagga
    36661 gagatgggca aaggagttat tggcttggag tttgactcag aagtattggt caacaaggct
    36721 ccaacccttc aattggcaaa tggtaaaaca gcgactttcc taacccagta tgatagcaaa
    36781 accttgttgt ttgcagtaga taaggaagat atcggacagg aaattattgg tatagctaaa
    36841 ggaagcatcg aaagtatgca taatcttcct gtaaatctag caggtgccag agttcctggc
    36901 ggagtaaatg gtagcaaagc agcggtgcat gaagttccag aatttacagg gggagttaat
    36961 ggtacagagc cagctgttca tgaaatcgca gagtataagg gatctgattc gcttgtaact
    37021 cttactacaa aagaagatta tacttacaaa gctcctcttg ctcagcaggc acttcctgaa
    37081 acaggaaaca aggagagtga cctcctagct tcactaggac taacagcttt cttccttggt
    37141 ctgtttacgc tagggaaaaa gagagaacaa taagagaaga attctaaaca tttgattttg
    37201 taaaaataga aggagatagc aggttttcaa gcctgctatc tttttttgat gacattcagg
    37261 ctgatacgaa atcataagag gtctgaaact actttcagag tagtctgttc tataaaatat
    37321 agtagattga aataagatgt gaacaactct atcaggaaag tcaaattaat ttatagaaaa
    37381 attttagcag tcaaggtgta ctgttataga ttcaatatat tatgtgacta ttaaccttct
    37441 cttctcctaa aattgacttt cttgttttct tatcttgtcc actcgaaaca agtattgtaa
    37501 gaatttgatt atttttgaaa gtacttttaa tatacttgat atagttaaaa aagatttgaa
    37561 actaaattcc aaattagaaa aagacttgaa atactaaaaa aaaaaagtat actctaattg
    37621 aaaacggtaa caaaactaat ttagagaatg aaatatagag tatttctctc ttaaaagttt
    37681 ttggtgaaac gagatgtaga aaggagattt agccaaagag tctattagtg ctagaataat
    37741 agattagaat tattttagaa aaacgaagtg agcagcttat aaattcaagt ccccaaatag
    37801 attcatacta gtatcttttg caaaaaataa agggcgactt ccttcatgaa tatcaatttc
    37861 atctataagg aaggtagcta attgaactaa cttatttatt ctgtttgtcg ctagaaaaat
    37921 cagacctcct tgtgaagatt gaggagatac tgaatgaaaa tcaaagaaga aactagcaag
    37981 ctagtagcag attgcccaaa acaccgcctt gaggttgtag ataagactga cctatataat
    38041 ccaaggtgaa gcgactgtgg tttgaagaga ttttcaaaga gtataggcta gagagtagtg
    38101 tttttatgtc cttctagtag aaaatgctag acagaagaat ggggaacttg gataggaaaa
    38161 atagattgag aaaggaggtt agaagagatg attattacaa aaattagcca tttaggaact
    38221 tatgtgggag taaatccaca ttttgcaaca ttaatagatt ttctagaaaa aacaggacta
    38281 gaaaatttaa cagaaggttc gattgctatc gatggtaatc gattgtttgg gaattgcttt
    38341 acttatctag cagatggtca agcaggggct ttctttgaaa cacaccaaaa atatttggat
    38401 attcatttag ttttggaaaa cgaagaagcc atggctgtta catcgccgga aaatgtaagc
    38461 gttacccaag aatatgatga agagaaagat attgaattat acacagggaa agtggaacag
    38521 ttggttcagt tgagagctgg cgaatgcctc atcacttttc cagaagattt acatcaaccc
    38581 aaggttcgta taaatgatga acctgtgaaa aaagttgtct ttaaagttgc gatttcttaa
    38641 tgtagaaaga gaagaacgat gaaaaaaatg agaaagtttt tatgtctagc tggaattgcg
    38701 ctagcggctg ttgccttggt agcttgttca ggaaaaaaag aagctacaac tagtactgaa
    38761 ccaccaacag aattatctgg tgagattaca atgtggcact cctttactca aggaccccgt
    38821 ttagaaagta ttcaaaaatc agcagatgct ttcatgcaaa agcatccaaa aacgaaaatc
    38881 aagattgaaa cattttcttg gaatgacttc tatactaaat ggactacagg tttagcaaat
    38941 ggaaatgtgc cagatatcag tacagctctt cctaaccaag taatggaaat ggtcaactca
    39001 gatgctttgg ttccgctaaa tgattctatc aagcgtattg gacaagataa atttaacgaa
    39061 actgccttaa atgaagcaaa aatcggagat gattactact ctgttcctct ttattcacat
    39121 gcacaagtca tgtgggttag aacagatttg ttaaaagaac ataatattga ggttcctaaa
    39181 acttgggatc aactctatga agcttctaaa aaattgaaag aagctggagt ttatggcttg
    39241 tctgttccgt ttggaacaaa tgacttaatg gcaacacgtt tcttgaactt ctacgtacgt
    39301 agtggtggag gaagcctctt aacaaaagat cttaaagcag acttgacaag ccaacttgct
    39361 caagatggta ttaaatactg ggttaaattg tataaagaaa tctcacctca agattctttg
    39421 aactttaatg tccttcaaca agctaccttg ttctatcaag gaaaaacagc atttgacttt
    39481 aactctggct tccatatcgg aggaattaat gccaacagtc ctcaattgat tgattcgatt
    39541 gatgcttatc ctattccaaa aatcaaagag tctgataaag accaaggaat tgaaacctca
    39601 aacattccaa tggttgtttg gaaaaattca aaacatccag aagttgctaa agcattctta
    39661 gaagcacttt ataatgaaga agactacgtt aaattccttg attcaactcc agtaggtatg
    39721 ttgccaacta ttaaggggat tagcgattct gcagcctata aagaaaatga aactcgtaag
    39781 aagtttaaac atgctgaaga agtaattact gaagctgtta aaaaaggtac tgctattggt
    39841 tatgaaaatg gaccaagtgt acaagctggt atgttgacta accaacacat tattgaacaa
    39901 atgttccaag atatcattac aaatggaaca gatcctatga aagcagcaaa agaagcagaa
    39961 aaacaattaa acgatttatt tgaggctgtt cagtagatgt aaaagactag aaaataggtg
    40021 ggctagtgag ctgaaaagct ctagcccaat cttgtaaaag aagggagaag gagaatggtt
    40081 aaagaacgta atttaactcg ctggatattt gttttgccag ctatgattat cgtaggatta
    40141 ctctttgttt atccgttttt ctcgagtatt ttttatagct ttaccaataa gcatttgatt
    40201 atgcctaatt ataaatttgt tggtttggct aactataaag ctgtgctatc agatcccaac
    40261 ttctttaatg cgttctttaa ttcaattaag tggaccgttt tctcattagt tggtcaagtt
    40321 ttagtagggt ttgtattggc tttagctctt cacagagtac gccacttcaa gaaattatat
    40381 aggaccttat tgattgttcc ttgggcattt cctaccatcg ttattgcctt ttcttggcag
    40441 tggattctaa acggggttta tggctactta cctaatctaa tcgtaaaatt aggtttaatg
    40501 gaacatacac ctgcattttt gacagatagt acatgggcat tcctatgttt ggtgtttatc
    40561 aacatttggt ttggagcacc aatgattatg gttaatgtgc tttcagcttt gcaaacagta
    40621 ccagaagaac aatttgaggc tgctaagata gatggtgctt caagttggca ggtgttcaag
    40681 tttatcgtct ttccacatat taaagtggtt gtaggacttc tagttgtttt gagaactgta
    40741 tggatcttta ataactttga cattatctac ctcattactg gtggtggacc agccaatgct
    40801 acaacgacgc ttccaatttt tgcttacaac ctgggctggg gaactaaatt gttgggtcgt
    40861 gcttcagcag ttacagtatt gctctttatc ttcttggtgg cgatttgctt tatctacttt
    40921 gctatcatca gtaagtggga aaaggagggt agaaaataat gaagaagaaa tccagtattt
    40981 atttagatat tctctcacat gtacttttag ttggtgcgac catcgttgca attttcccat
    41041 tggtatggat tatcatatct tctgtcaaag ggaaagggga attaactcag tatccaacac
    41101 gattttggcc tgaacagttt acattagatt atttcactca tgttatcaac gatttgcact
    41161 tcattgataa cattcgaaac agtttaatca ttgccttggc tacaaccctc attgcgatta
    41221 ttatttctgc tatggcagcc tatggtattg ttcgattctt tcctaaattg ggagcaatca
    41281 tgtcgagact actcgtcatt acctacattt tcccaccaat tttgttagca attccctatt
    41341 caattgccat tgctaaagtt gggttaacaa atagtttatt tggcttgatg atggtttatc
    41401 tatcttttag tgttccatat gcagtttggc tcttagttgg atttttccaa acagttccaa
    41461 ttggaattga agaagcggct agaattgatg gtgcaaataa atttgttacg ttttataaag
    41521 ttgtgctacc gattgtagca ccaggtattg tagcaacagc tatttataca tttatcaatg
    41581 cttggaatga attcctgtat gccttgattt tgattaacaa tacaggaaag atgacagtag
    41641 cagtagccct tcgttcactt aatggttcag aaatactaga ctggggagat atgatggcag
    41701 cgtctgttat tgtagttctt ccatcaatta ttttcttctc tatcatccaa aataagattg
    41761 caagtggatt atcagaagga tctgtgaagt agacgaaaga aggaaaaaaa tgaataaaag
    41821 aggtctttat tcaaaactag gaatttctgt tgtaggcatt agtcttttaa tgggagtccc
    41881 cactttgatt catgcgaatg aattaaacta tggtcaactg tccatatctc ctatttttca
    41941 aggaggctca tatcaactga acaataagag tatagatatc agccctttgt tattagataa
    42001 attgtctgga gagagtcaga cagtagtaat gaaatttaaa gcagatgaac caaactctct
    42061 tcaagctttg tttggcctat ctaatagtaa agcaggcttt aaaaataatt acttttcaat
    42121 tttcatgaga gattctggtg agataggtgt agaaataaga gacgcccaaa agggaataaa
    42181 ttatttattt tccagaccag cttcattatg gggaaaacat aaaggacagg cagttgaaaa
    42241 tacactagta tttgtatctg attctaaaga taaaacatac acaatgtatg ttaatggaat
    42301 agaagtgttc tctgaaacag ttgatacatt tttgccaatt tcaaatataa atggtataga
    42361 taaggcaaca ctaggagctg ttaatcgtga aggcaaggaa cattacctcg caaaaagaag
    42421 tattgatgaa atcagtctat ttaacaaagc aattagtgat caggaagttt caaatattcc
    42481 cttgtcaaat ccatttcagt taattttcca atcaggagat tctactcaag ctaactattt
    42541 tagaataccg acactatata cattaagtag tggaagagtt ctatcaagta ttgatgcacg
    42601 ttatggtggg actcatgatt ctaaaagtaa gattaatatt gccacttctt atagtgatga
    42661 taatgggaaa acgtggagtg agccaatttt tgctatgaag tttaatgact atgaggagca
    42721 gttagtttac tggccacgag ataataaatt aaagaatagt caaattagtg gaagtgcttc
    42781 attcatagat tcatccattg ttgaagataa aaaatctggg aaaacgatat tactagctga
    42841 tgttatgcct gcgggtattg gaaataataa tgcaaataaa gccgactcag gttttaaaga
    42901 aataaatggt cattattatt taaaactaaa gaagaatgga gataacgatt tccgttatac
    42961 agttagagaa aatggtgtcg tttatgatga aacaactaat aaacctacaa attatactat
    43021 aaatgataag tatgaagttt tggagggagg aaagtcttta acagtcgaac aatattcggt
    43081 tgattttgat agtggctctt taagagaaag gcataatgga aaacaggttc ctatgaatgt
    43141 tttctacaaa gattcgttat ttaaagtgac tcctactaat tatatagcaa tgacaactag
    43201 tcagaataga gaagagagtt gggaacaatt taagttgttg cctccgttct taggagaaaa
    43261 acataatgga acttacttgt gtcctggaca aggtttagca ttaaaatcaa gtaacagatt
    43321 gatttttgca acatatacta gtggagaact aacctatctc atttctgatg atagtggtca
    43381 aacatggaag aaatcctcag cttcaattcc gtttaaaaat gcaacagcag aagcacaaat
    43441 ggttgaactg agagatggtg tgattagaac attctttaga accactacag gtaagatagc
    43501 ttatatgact agtagagatt ctggagaaac atggtcggaa gtttcgtata ttgatggaat
    43561 ccaacaaact tcatatggca cacaagtatc tgcaattaaa tactctcaat taattgatgg
    43621 aaaagaagca gtcattttga gtacaccaaa ttctagaagt ggccgtaagg gaggtcaatt
    43681 agttgtcggt ttagtcaata aagaagatga tagtattgat tggaaatacc actatgatat
    43741 tgatttgcct tcgtatggtt atgcctattc tgcgattaca gaattgccaa atcatcacat
    43801 aggtgtactg tttgaaaaat atgattcgtg gtcgagaaat gaattgcatt taagcaatgt
    43861 agttcagtat atagatttgg aaattaatga tttaacaaaa taaaggagaa aaacatggtt
    43921 aaatacggtg ttgttggagc agggtatttt ggagctgaat tggctcgcta catgcaaaag
    43981 aatgatggag cagagattac tcttctctat gatccagata atgcagaggc gattgcagaa
    44041 gaattgggag caaaagtagc aagttcctta gatgagttgg tttctagcga tgaagtagat
    44101 tgtgttatcg tcgcaactcc aaataatctt cataaggaac cggttattaa ggctgcacag
    44161 catggtaaaa atgttttctg tgaaaaacca attgcgcttt cttatcaaga ttgtcgcgag
    44221 atggtagatg cgtgtaaaga aaacaatgta acctttatgg caggacatat tatgaatttc
    44281 tttaatggtg ttcatcatgc aaaagaactc attaatcaag gagttatcgg agacgttcta
    44341 tattgtcata cagctcgtaa tggttgggaa gaacaacaac cgtcagtgtc atggaaaaaa
    44401 attcgtgaaa aatcaggtgg tcacttgtat caccacatcc atgaattgga ttgcgttcaa
    44461 ttccttatgg ggggcatgcc tgaaactgta accatgacag gtggaaatgt ggcccatgaa
    44521 ggtgaacatt tcggtgatga agatgatatg atttttgtca atatggaatt ttctaataag
    44581 cgttttgcct tgttagaatg gggttcagct tatcgttggg gtgaacatta tgtcttaatc
    44641 caaggaagca aaggtgccat ccgcttagac ttattcaact gtaaaggaac tcttaagcta
    44701 gatgggcaag aaagctattt cttgattcac gaatcgcaag aagaagatga tgatcggact
    44761 cgtatctatc atagtacaga gatggatgga gcaattgctt atggtaaacc aggtaaacgt
    44821 actccattat ggctatcatc tgtcattgat aaagaaatgc gctatctgca tgagattatg
    44881 gaaggagctc cagtatcaga agaatttgca aaacttttga caggtgaagc tgccctagaa
    44941 gcaattgcta ctgcagatgc ttgtacccag tctatgtttg aagatcgcaa agtaaaattg
    45001 tcagaaattg taaaataaat tttggtattc tcctatttat aggtcgactt gctcctctga
    45061 aagtactttt agaggagctg tttgactttg ctagtttttg aaactgaaat ctattatact
    45121 acaaactatt gaaagcgttt taattttaag gtataataat ctcatagaaa taaagaaaag
    45181 gaggaaagag gatgccacag attagcaaag aagccttgat tgagcaaatc aaagatggaa
    45241 tcatcgtttc ttgtcaggct cttcctcatg aaccgcttta tacagaagcg ggaggggtga
    45301 ttcccttgct ggtcaaagcg gctgagcaag gtggagcagt cggtatccga gcaaacagtg
    45361 ttcgcgatat caaggaaatt aaggaagtca ctaaacttcc aatcattggg attatcaaac
    45421 gtgattatcc acctcaggaa cccttcatca cggctactat gaaagaagtt gatgaattgg
    45481 cagaactgga catcgaggtg attgctctgg attgtaccaa gcgtgaacgc tacgatggtt
    45541 tggaaattca agagttcatt cgtcaggtta aggagaaata tcctaatcag cttttgatgg
    45601 ctgatactag tatcttcgaa gaagggctag cagctgtaga agcaggaatt gactttgtcg
    45661 gaacaacctt atcaggctac acatcctaca gtccaaaagt agacggtcca gattttgaat
    45721 tgattaagaa actctgtgat gccggtgtag atgtcattgc agaaggaaaa attcatacac
    45781 cagaacaagc caaacaaatc cttgaatatg gagtgcgagg catcgttgtt ggtggcgcca
    45841 ttactagacc aaaagagatt acagaacgct tcgttgctag tcttaaataa gatgtgaggg
    45901 ggagttttat gtttaaagtt ttacaaaaag ttggaaaagc ttttatgtta cctatagcta
    45961 tacttcctgc agcaggtcta cttttgggga ttggtggtgc actttcaaac ccaaccacga
    46021 tagcaactta tccaatacta gacaatagta tttttcaatc aatattccaa gtaatgagct
    46081 ctgcaggaga ggttgtattc agtaatttgt cactacttct ctgtgtggga ttatgtattg
    46141 gcttagcgaa acgagataaa ggaaccgctg cgttagcagg agtaactggt tacttagtta
    46201 tgactgcaac gatcaaagct ttagtaaaac tttttatggc agaaggatct gcaattgata
    46261 ctggagttat tggagcatta gttgtcggaa tagttgccgt atatttgcac aaccgatata
    46321 acaatattca attaccttcc gctttaggat tctttggagg ttcacgcttc gttcctattg
    46381 ttacatcgtt ctcttctatc ttgattggct ttgtcttctt tgttatttgg ccacctttcc
    46441 aacaacttct tgtttctaca ggtggatata tttctcaggc gggtccaatt ggaacttttc
    46501 tatatggatt tttaatgaga ctttctggag cagtaggctt acatcatata atttacccta
    46561 tgttttggta tactgaactt ggtggtgttg aaactgttgc aggacaaaca gtggttggag
    46621 ctcaaaaaat attttttgct caattagccg atttggccca ttctggatta tttacagaag
    46681 gaacaaggtt ttttgcaggt cgtttctcaa caatgatgtt cggtttaccg gctgcctgtt
    46741 tagcgatgta ccatagtgtt cctaaaaatc gtcgtaaaaa atacgcgggt ttgtttttgg
    46801 gagttgcttt aacatctttt attaccggta ttacagaacc aattgaattt atgtttctat
    46861 tcgttagtcc ggttctatat gttgttcacg cattccttga tggtgttagc ttctttattg
    46921 cagacgtctt aaatatttca ataggaaaca cattttcagg aggtgtaatc gatttcactt
    46981 tatttggaat tttgcagggg aacgctaaga cgaattgggt tcttcagatt ccatttggac
    47041 ttatttggag tgttttgtat tatattattt ttagatggtt cattactcaa ttcaacgttc
    47101 taacgccagg gcgaggagaa gaagtagatt ctaaagaaat ttctgaatcc gcagattcaa
    47161 cttcaaatac tgcagattat ttaaaacagg atagcctaca aattatcaga gccttgggtg
    47221 gatcaaataa tatagaagat gtagatgctt gtgtgacacg tttacgtgta gctgtaaaag
    47281 aagttaatca agttgataaa gcacttttaa aacaaattgg tgcagttgat gtcttagaag
    47341 tgaagggtgg cattcaagca atatatggag caaaagcaat cttatataaa aatagtatta
    47401 atgaaatttt aggtgtagat gattaagtac ttactgactt aataaaaaac agaggagagt
    47461 gatggatgag taggatgaaa tgaaatcgca tacaagaaat aaagaactca ttatccaagt
    47521 tggatacgct tattacatag gagaatacaa atgaaattta gaaaattagc ttgtacagta
    47581 cttgcgggtg ctgcggttct tggtcttgct gcttgtggca attctggcgg aagtaaagat
    47641 gctgccaaat caggtggtga cggtgccaaa acagaaatca cttggtgggc attcccagta
    47701 tttacccaag aaaaaactgg tgacggtgtt ggaacttatg aaaaatcaat catcgaagcg
    47761 tttgaaaaag caaacccaga tataaaagtg aaattggaaa ccatcgactt caagtcaggt
    47821 cctgaaaaaa tcacaacagc catcgaagca ggaacagctc cagacgtact ctttgatgca
    47881 ccaggacgta tcatccaata cggtaaaaac ggtaaattgg ctgagttgaa cgacctcttc
    47941 acagatgaat ttgttaaaga tgtcaacaat gaaaacatcg tacaagcaag taaagctgga
    48001 gacaaggctt atatgtatcc gattagttct gccccattct acatggcaat gaacaagaaa
    48061 atgttagaag atgctggagt agcaaacctt gtaaaagaag gttggacaac tgatgatttt
    48121 gaaaaagtat tgaaagcact taaagacaag ggttacacac caggttcatt gttcagttct
    48181 ggtcaagggg gagaccaagg aacacgtgcc tttatctcta acctttatag cggttctgta
    48241 acagatgaaa aagttagcaa atatacaact gatgatccta aattcgtcaa aggtcttgaa
    48301 aaagcaacta gctggattaa agacaatttg atcaataatg gttcacaatt tgacggtggg
    48361 gcagatatcc aaaactttgc caacggtcaa acatcttaca caatcctttg ggcaccagct
    48421 caaaatggta tccaagctaa acttttagaa gcaagtaagg tagaagtggt agaagtacca
    48481 ttcccatcag acgaaggtaa gccagctctt gaataccttg taaacggatt tgcagtattc
    48541 aacaataaag acgacaagaa agtcgctgca tctaagaaat tcatccagtt tatcgcagat
    48601 gacaaagagt ggggacctaa agacgtagtt cgtacaggtg ctttcccagt ccgtacttca
    48661 tttggaaaac tttatgaaga caaacgcatg gaaacaatca gcggctggac tcaatactac
    48721 tcaccatact acaacactat tgatggtttt gctgaaatga gaacactttg gttcccaatg
    48781 ttgcaatctg tatcaaatgg tgacgaaaaa ccagcggatg ctttgaaagc cttcactgaa
    48841 aaagcaaacg aaacaatcaa aaaagctatg aaacaatagt ccttagttat tctataaaaa
    48901 gtagtttttt aaagaaccta agagtgtata cccccttttc cctctacaca gatagtgtaa
    48961 gaaaaggggg cttttgttta aaatgtaaga aactgtcacg aaattaaaat gaagttctta
    49021 cataagcgaa tcataaaaaa tttcattttg attttaaaac agttcaagaa agtcaaaaaa
    49081 ttattctatt tgaaagagag gtgccgactg tgaaagtcaa taaaatccgt atgcgggaaa
    49141 cagtgatttc ctacgctttc ctagcaccag tattattctt ctttgtcatc tttgtgttgg
    49201 ctccgatggt gatgggcttc attacaagtt tctttaacta ctcaatgact aaatttgagt
    49261 ttgtaggctt ggataactat atccgtatgt ttaaagatcc tgtctttaca aaatctctga
    49321 ttaacacagt tattttggtt attggatctg taccagttgt tgttctattc tcactctttg
    49381 tagcatctca gacctatcat caaaatgtca ttgccagatc cttctaccgt ttcgtcttct
    49441 tccttcctgt tgtaacgggt agtgttgccg tgacagttgt ttggaaatgg atttatgacc
    49501 cactatcagg gattctaaac tttgtcctta agtccagcca catcatcagc caaaacattt
    49561 cttggttggg agataaaaac tgggcattga tggcgattat gattattctc ttgaccactt
    49621 cagttggtca gcccatcatc ctttatatcg ctgccatggg gaatattgac aattcactgg
    49681 ttgaagcggc gcgtgttgat ggtgcaactg agtttcaagt tttttggaag attaaatggc
    49741 caagccttct tccaacaact ctttatattg caatcatcac aacaattaac tcattccagt
    49801 gtttcgcctt gattcagctt ttgacatctg gtggtccaaa ctactcaaca agtaccttga
    49861 tgtactacct ttacgaaaaa gccttccaat tgacagaata cggctatgcc aacacaattg
    49921 gtgtcttctt ggcagtcatg attgctatcg taagctttgt tcaatttaaa gtacttggaa
    49981 acgacgtaga atactaaaga aaggagacag ctatgcaatc tacagaaaaa aaaccattaa
    50041 cagcctttac tgttatttca acaatcattt tgctcttgtt gactgtgctg ttcatctttc
    50101 cattctactg gattttgaca ggggcattca aatcacaacc tgatacaatt gttattcctc
    50161 ctcagtggtt ccctaaaatg ccaaccatgg aaaacttcca acaactcatg gtgcagaacc
    50221 ctgccttgca atggatgtgg aactcagtat ttatctcatt ggtaaccatg ttcttagttt
    50281 gtgcaacctc atctctagca ggttatgtat tggctaaaaa acgtttctat ggtcaacgca
    50341 ttctatttgc tatctttatc gctgctatgg cgcttccaaa acaagttgtc cttgtaccat
    50401 tggtacgtat cgtcaacttc atgggaatcc acgatactct ctgggcagtt atcttgcctt
    50461 tgattggatg gccattcggt gtcttcctca tgaaacaatt tagtgaaaat attcctacag
    50521 agttgcttga atcagctaaa atcgacggtt gtggtgagat tcgtaccttc tggagtgtag
    50581 ccttcccgat tgtgaaacca gggtttgcag cccttgcaat ctttaccttc atcaatactt
    50641 ggaatgacta cttcatgcag ttggtaatgt tgacttcacg taacaatttg accatctcac
    50701 ttggggttgc gaccatgcag gctgaaatgg caaccaacta tggtttgatt atggcaggag
    50761 ctgcccttgc tgctgttcca atcgtcacag tcttccttgt cttccaaaaa tccttcacac
    50821 agggtattac tatgggagcg gtcaaaggat aatactctgc gaaaatcgaa tgcaaactac
    50881 gtcagcttca ccttgccata cttaagtatt gcctgcggtt agcttcctag tttgttcttc
    50941 aattttcatt gagtatagga aaatcaatct atcaagatac agaagtatat tttatagatt
    51001 tagagaatat agaagttata agtgtctaca aaatggaggg tatgcagtta ctttatgaag
    51061 ttttgtcaga cacttatcaa cttaagaatg attttagtta attatcagaa acgaaggaaa
    51121 gagtatgatt tttgacgatt tgaaaaacat caccttttac aaagggattc atcccaattt
    51181 agacaaagct atcgactacc tctaccaaca tcgtaaagat tcattcgaat taggaaagta
    51241 tgagattgat ggagataagg tctttctagt tgttcaggaa aatgtcctca atcaagttga
    51301 gaataatcaa tttgaacacc ataagaacta tgcagatttg catttgctga tagaagggca
    51361 tgaatattcg agctacggtt cacgtatcaa agacgaggca gtagcattcg acgaagcgac
    51421 tgacattggc tttgttcatt gtcatgaaca ctacccactc ttgttgggtt atcataattt
    51481 tgcgattttc ttcccaggtg agccacatca gccaactggc tatgcaggta tggaagaaaa
    51541 ggttcgaaaa tatctcttta aaattttgat tgattaaaaa taggatgaat tgtttttttg
    51601 taaagctttg acaatactct accatgaaat tgatctttat gaggtagaga aatgagaata
    51661 aaatatttaa aaattggtat cttctaagca tgctgcaaga gctagtttct tagatggaca
    51721 ggaaattaca gttgatgaga tgtcctggat aattaatggc attgtgaatg cattgattgg
    51781 tagatacata aaattaggta cttatgcagc taagtatggt attggtatag cacgctcgat
    51841 cttaagtagg gcagctgcaa ctgcagcagc aagagtagga ttactgacca agatttctgg
    51901 atggatttta cgagtagctg tgaatgtagc tgatgtatat ggtaatttcg ccaacaatat
    51961 tgctgcagct tgggatgcat atgataaaat tcctaacaat ggtcgtataa acttttaaaa
    52021 tacgagaatg aaagcacttt gtattttttt attgaatatg ttagcttgga cagtgcttgc
    52081 aatgataatt cgtggagggc tagatggatt tgataggcat acttggagta ctattttaat
    52141 tgcttcgctg ttcggggtat atgattataa gcccatagat aaaaatagaa aaaagtccaa
    52201 aagaaaaaag aaaaaataga tttgttcatg gtagggactt atgaaagctt tactgacaaa
    52261 aaagaaaaca gtttacaaag aaaaatgatg gaggagcaaa catggcacaa aaaggagtaa
    52321 gccttatcaa ggcagcattt gatacagata actttctcat gcgttttagt gagaaggtct
    52381 tggacatcgt gacagccaat cttctttttg tcgtctcttg tttacccatc gtgacgattg
    52441 gagtggctaa aatcagcctc tacgagacca tgttcgaagt taagaagagc agacgggtgc
    52501 ctgtttttaa aatctatcta agatctttca agcaaaatct gaaactaggt cttcagctgg
    52561 gtttaatgga gttaggaatt gtgtttctta ccctttcaga tctctatctt ttctggggtc
    52621 aaacagctct gcccttccaa ttgctgaaag ccatttgttt aggtattctg atttttctta
    52681 ctatcgtgat gctggctagt taccctatcg cggcacgtta tgacctatct tggaaagaaa
    52741 ttcttcaaaa aggattgatg ttggctagtt ttaactttcc ttggttcttc ctcatgttag
    52801 ccattcttgt cctcattgtg atggttcttt atctgtccgc cttcagtcta ctcttaggtg
    52861 gctcagtctt cctacttttt gggtttggac tattggtctt tatccagact ggattgatgg
    52921 agaaaatttt cgcaaaatac caataggagc tttatttctg aaactacttt caaaggctcc
    52981 aaacgctatt ctataagcga gaaactaaaa tctaagtgtc tataagatat tgaaagcctt
    53041 ttcggtaaag tgtatactaa agaagtaaag aaaaatctgc tttagcagaa aataaaaata
    53101 ataggagaaa atctatgtca gatttgaaaa aatacgaagg tgtcattcca gccttctacg
    53161 catgttatga tgagcaagga gaagtaagtc cagagcgtac gcgtgccttg gttcaatact
    53221 tcattgataa aggtgttcaa ggtctttatg tcaatggttc ttctggtgaa tgtatctacc
    53281 aaagcgttga agatcgcaag ttgattttgg aagaagtcat ggcggtagct aaaggtaaat
    53341 tgaccattat tgcccatgtt gcctgcaaca atactaaaga cagtatggaa cttgctcgcc
    53401 atgctgaaag cttgggagta gatgctattg caacgattcc accaatttat ttccgcttgc
    53461 cagaatactc agttgccaaa tactggaacg atatcagttc tgcagctcca aacacagact
    53521 acgtgattta caacattcct caattggcag gggttgcttt gactccaagc ctttacacag
    53581 aaatgttgaa aaatcctcgt gttatcggtg tgaagaactc ttctatgcca gttcaagata
    53641 tccaaacctt tgtcagcctt ggtggggaag accatatcgt ctttaacggt ccagatgaac
    53701 agttcctagg tggtcgcctc atgggtgcca aagctggtat cggtggtact tatggtgcta
    53761 tgccagaact cttcttgaaa ctcaaccaat tgattgcgga taaagattta gaaacagcgc
    53821 gtgagttaca gtatgctatc aacgctatca ttggtaaatt gactgctaca catggaaata
    53881 tgtacggtgt catcaaagaa gttttgaaga tcaatgaagg cttgaatatt ggttctgttc
    53941 gttcaccatt gacaccagtg actgaagaag atcgtccagt tgtagaagcg gctgctgcct
    54001 tgattcgtga aaccaaggag agcttcctct aatctaaaag gaggtattta tgacatacta
    54061 cgttgcaatt gatatcggtg gaaccaacat caagtatggt ttggttgatc aagaggggca
    54121 acttcttgaa tcgcatgaaa tgccaactga ggcgcataag ggtggacctc atatcttaca
    54181 aaagaccaaa gatatcgtag ctagttattt agaaaaaggc ccagtagcag gtgttgccat
    54241 atcttctgct gggatggtgg atccggataa gggtgagatt ttctatgctg ggccgcaaat
    54301 ccctaactac gcaggcaccc agttcaaaaa ggaaatcgaa gaaagcttta ctattccttg
    54361 tgagattgaa aatgatgtca actgtgcagg tcttgctgag gcagtatctg gttcaggcaa
    54421 gggagcaagt gtgacacttt gcttgaccat tggaaccggt atcggtggtt gcttgattat
    54481 ggataggaaa gtcttccatg gttttagcaa ttcagcctgt gaagtcgggt atatgcatat
    54541 gcaggatgga gcttttcaag acttggcttc tacaacagct ttagtgaaat atgtagctga
    54601 agcccatgga gaagatgttg atcagtggaa tggccgtaga attttcaaag aagccactga
    54661 aggaaacaaa atctgcatgg aaggtattga ccgtatggtt gactatctag gaaaaggtct
    54721 ggcaaatatt tgctacgttg ccaatccaga agtggttatt cttggtggtg gtatcatggg
    54781 gcaagaggct atcctcaaac ctaagatccg cacagccttg aaagaggctt tggtaccaag
    54841 tttagcagaa aaaacacgat tagaatttgc ccatcaccaa aatacagcag ggatgttggg
    54901 tgcatattat cattttaaga caaaacaatc ctagtttggc tcagccaaac taggattttc
    54961 ttacacgttt ttgtctacga tagccgttga gttttttatt ttcccagtag ctattaaaga
    55021 ttttttcctt gctttcgcga ttgatttcca aaaagtaggc ataaatcaaa tcgataaaga
    55081 agagcatagg aagttgagcg gatattcgtt ggatatagga gggttggctg tgggtggcta
    55141 caagaacagt ctctgtatag gtctggctat ctttattggg aacacttgta aagagtacag
    55201 tctttgcccc catctccttg gcatctaata gactatctaa aatagaagga gttgagcctg
    55261 aaagtgagaa gcccagtact agacaatttt catccatgat gctggttgtc caggcaaagc
    55321 cgtcttggtc tgtcaaagct tcgcagacca cacctagtcg cataaaacgt aatttcattt
    55381 cacgggcgac gaggccagaa ctccctgttc caaagaagta gatacgctca gcatcttcga
    55441 ttagctgggc aattcgctct agttggattt cgtcaatcaa gtcttgtgtt tgttccctca
    55501 tattgctata acttctgagg actcgtttgg tcagtggact gtgcttggag actcggttgg
    55561 cttgattttc tgcctgatgt tggtattgga aaataaattc tcggtagcca gtaaagccac
    55621 actttttagc aaagcgggtc aaagcagctt gagaaatatg taatttttgg gtgacttgtt
    55681 gagaagataa atcatctgta atcgtttcag cttgcaaaaa atagcgagcg atttcttgct
    55741 ctaggtctgt catttcttca aaatgtgaat caatgatagt tgcgatatct ggtttgtcca
    55801 tagggaaagc tcctttacat gagtcatact ggaagactag atcagggaat agtcacactt
    55861 cattataaca cataatataa ggatagataa ataaaaacgc atctctgttt taaaaacgaa
    55921 aaaatcgaaa aagcttctct cttttccata attttctact caaattgtgg tacaattaag
    55981 agtaagattt taagttagaa atgagactga tttgtatgag aaaatttaac agccattcga
    56041 ttccgattcg gcttaattta ttgttttcaa tcgtcatttt actctttatg accattattg
    56101 gtcgtttgtt gtatatgcag gttttgaaca aggattttta cgaaaaaaag ctagcctcag
    56161 ctagtcagac caagattaca agcagttcag cccgtgggga aatttatgat gctagtggaa
    56221 aacctttggt agaaaatacg ttaaagcagg ttgtttcctt tacgcgtagc aataaaatga
    56281 cggctacaga cttaaaagaa acagctaaaa agttactgac ttatgtgagc atcagttctc
    56341 caaatttgac aaaacgccag ctggcggatt actatttggc tgatcctgaa atctataaaa
    56401 aaacagtgga agctctccca agcgagaaac gcttggattc agatggcaat cgtctatccg
    56461 aatcagaact gtataacaat gcggtcgata gtgtccaaac aagtcaacta aactatacag
    56521 aggatgaaaa gaaagaaatc tatcttttta gtcagttaaa tgctgttgga aactttgcga
    56581 caggaaccat tgctacagat cctctaaatg attctcaggt ggctgttatt gcctctattt
    56641 caaaggagat gcctggcatt agtatttcta cttcttggga tagaaaggtt ttggaaactt
    56701 ccctttcttc tatagttggg agtgtatcca gtgaaaaagc tggtctccca gcggaagaag
    56761 cagaagccta tcttaaaaaa ggctattctc taaatgaccg tgtaggaacc tcctatttgg
    56821 aaaagcaata tgaagagacc ttacaaggaa aacgatcggt aaaagaaatc catctggata
    56881 aatatggcaa tatggaaagc gtggatacaa ttgaggaagg tagtaaggga aacaatatca
    56941 aactgaccat tgatttggcc ttccaagata gcgtggatgc tttgctgaaa agttatttca
    57001 attctgagct agaaaatggt ggagccaagt attctgaagg tgtctatgca gtcgccctta
    57061 acccaaaaac aggtgcggtt ttgtctatgt cagggattaa acatgacttg aaaacgggag
    57121 agttgacgcc tgattccttg ggaacggtaa ccaatgtctt tgttccaggt tcggttgtca
    57181 aggcggcgac catcagctca ggttgggaaa atggagtctt gtcaggaaac cagaccttga
    57241 cagaccagtc cattgtcttc caaggttcag ctcccatcaa ttcttggtat actcaggctt
    57301 acggttcatt ccctatcaca gcggtccaag ctctggagta ttcatcaaat acctatatgg
    57361 tccaaacagc cttaggtctt atggggcaaa cctatcaacc caatatgttt gtcggcacca
    57421 gcaatctaga gtctgctatg gagaaactgc gttcaacctt tggcgaatat ggcttgggta
    57481 ctgcgacagg aattgaccta ccagatgaat ctactggatt tgttcccaaa gagtatagct
    57541 ttgctaatta cattactaat gcctttgggc agtttgataa ctatacgccg atgcagttgg
    57601 ctcagtatgt agcaactatt gcaaataatg gtgttcgtgt ggctcctcgt attgttgaag
    57661 gcatttatgg taataatgat aagggaggac tgggtgactt gattcagcaa ctgcaaccga
    57721 cagagataaa taaggtcaat atatccgact ccgatatgag catcttgcac caaggttttt
    57781 atcaggttgc ccatggtact agtggattga caactggacg tgccttttca aatggtgcct
    57841 tggtatccat tagcggaaaa acaggtacag ccgaaagcta tgtggcagat ggtcagcaag
    57901 caaccaatac caatgcggtg gcctatgccc catctgataa tccccaaatc gctgtcgcag
    57961 tggtctttcc tcataatacc aatctaacaa atggtgtagg accttccatt gcgcgtgaca
    58021 ttatcaatct gtatcaaaaa taccatccaa tgaactagaa aggaaattat gctttatcca
    58081 acacctattg ccaagttgat tgacagttat tctaagttac caggtatcgg gattaagacg
    58141 gctacgcgtc tggcctttta tacgattggg atgtctgctg atgatgtcaa tgaatttgca
    58201 aaaaatctcc tttctgctaa gagagaattg acatattgtt ctatttgtgg acgtttgaca
    58261 gacgacgatc cttgttctat ctgtactgat ccgactcgtg accagacaac aattttagtt
    58321 cttgaggata gtagagatgt ggcagccatg gaaaatatcc aagaatacca tggactctat
    58381 catgtcctgc atggcctcat ttctcctatg aatggtatca gtccggacga tatcaatctc
    58441 aagagcctta tgactcgtct tatggatagt gaggtttcag aagtgattgt ggcgactaat
    58501 gctacagcgg atggtgaagc gacttccatg tatctttcac gtttgctcaa gccagctggt
    58561 atcaaggtta cgcgtctagc acgaggtctc gctgtgggag cggacattga gtatgcggac
    58621 gaagtgacac tcttacgagc gattgaaaat cggacagagt tgtaagtgta ggcaaattta
    58681 cgaactccct tcatttataa aaaatcaaag aggctgaaaa tcgttcctat cggcctcttt
    58741 ttgtatagtg tgatgagtag gatcgggttc aagttttaaa aaaccaagca aatatgatat
    58801 actaaagagc gagtattcta gtagaattag gacaaataat atgaaacaaa cgattattct
    58861 tttatatggt ggacggagtg cggaacgcga agtctctgtc ctttcagctg agagtgtcat
    58921 gcgtgcggtc aattacgacc gtttcacagt caagactttc tttatcagtc agtcaggtga
    58981 ctttatcaaa acacaggaat ttagtcatgc tccggggcaa gaagaccgtc tcatgaccaa
    59041 tgaaaccatt gattgggata agaaagttgc accaagtgct atctacgaag aaggtgcagt
    59101 ggtctttcca gtccttcacg ggccaatggg agaagatggc tctgttcaag gattcttgga
    59161 agttttgaaa atgccttacg ttggttgcaa cattttgtca tcaagtcttg ccatggataa
    59221 aatcacgact aagcgtgttc tggaatctgc tggtattgcc caagttcctt atgtggctat
    59281 cgttgaaggc gatgatgtga ctgctaaaat cgctgaagtg gaagaaaaat tggcttatcc
    59341 agtcttcact aagccgtcaa acatggggtc tagtgtcggt atttctaagt ctgaaaacca
    59401 agaagaactc cgtcaagcct taaaacttgc cttccgatat gacagccgtg tcttggttga
    59461 gcaaggagtg aatgcccgtg aaattgaggt tggcctcttg ggtaactacg atgtcaagag
    59521 cacgctacct ggagaagttg tcaaggacgt tgccttttat gactacgatg ccaagtatat
    59581 tgataacaag attactatgg atattcctgc caaaatcagt gatgatgtgg tagctgtcat
    59641 gcgtcaaaat gcagaaacag ccttccgtgc cattggtggc cttggtctat ctcgttgcga
    59701 tttcttctat acagataagg gagagatttt tctcaacgag ctcaatacca tgccaggttt
    59761 cacccagtgg tctatgtacc cactactttg ggacaatatg gggatcagct acccagaact
    59821 aatcgagcgt ttggttgacc ttgccaagga aagttttgac aagcgcgaag cgcatttgat
    59881 ataaaatgaa agagagggta gaagccagaa ccatcactgc aaggtgacta gagttctcgg
    59941 acttcagccc tttttaaagg agtagaaatg aaattaacaa tccatgaagt tgcccaagtt
    60001 gttggagcca aaaatgatat cagtatcttt gaggacaccc agttagaaaa agctgagttt
    60061 gatagtcgtt tgattggaac tggagattta tttgtgcccc taaaaggtgt gcgtgacggt
    60121 catgacttta tcgaaagagc ttttgaaaat ggtgcggcag caaccctgtc tgagaaagag
    60181 gttgcaaatc atccctacat tctagtagat gatgttttga ctgcctttca acaactagct
    60241 gcctactatc ttgaaaaaac agctgttgat gtctttgcgg ttacgggttc aaatggcaag
    60301 acaacgacta aggatatgtt ggcgcattta ctgtcaacaa gatacaagac ctacaaaaca
    60361 caaggcaatt acaataatga gattggcctt ccttacacag ttctccacat gcctgaagga
    60421 acagaaaagt tggtcttgga gatggggcag gaccacttgg gagatattca tctcttgtct
    60481 gaattggctc gtccaaaaac agccatcgtg accttggttg gagaagctca tttggccttt
    60541 ttcaaagatc gttctgagat tgctaaagga aaaatgcaaa ttgcagacgg aatggcttca
    60601 ggttccttgc ttttagcgcc ggctgaccct atcgtagagg actatttgcc aactgataaa
    60661 aaggtggttc gttttggtca aggagcagag ctggaaatta ctgacttggt tgagcgcaaa
    60721 gatagtctga ccttcaaggc caatttctta gagcaagtcc ttgatttgcc agtaactggc
    60781 aagtacaatg cgacaaatgc tatgattgca tcctatgttg ccttgcaaga aggagtttca
    60841 gaggagcaaa ttcgtttggc cttccaacat cttgaattga cgcgtaaccg taccgagtgg
    60901 aagaaagcag ccaatggagc agacatcttg tcagatgttt acaatgccaa tccaactgct
    60961 atgaagctga ttttagagac cttctctgcc attccagcca atgaaggtgg caagaaaatt
    61021 gcagtgttgg cggatatgaa ggaacttggt gtccagtctg ttcaacttca taatcagatg
    61081 attttgagcc tttcgccaga tgtgcttgat actgttattt tctacggaga agatattgct
    61141 gaattagccc aacttgctag tcaaatgttc ccaatcggac acgtttatta cttcaagaaa
    61201 acagaagacc aagaccaatt tgaagaccta gttaagcagg tcaaagaaag ccttggggct
    61261 catgaccaaa tcctgctcaa aggctctaac tctatgaatc tagccaagtt ggtagaaagt
    61321 ttagaaaatg aagacaagtg attttgtcaa gtatttgcaa agaatgattg ccattacaga
    61381 tactggatta acctttacaa aagatccgtt tgaccgtgag cgctacgaag acttgcgaga
    61441 ccttctatct gaaatgttga atcaagcatc agaccttgat tccgaagaag tggcagaagt
    61501 cttgaagcca acttctgctt atgcgactcc cttaatggac gtccgtgctt ggattgttga
    61561 ggacgaaaag atttgtctgg ttaggggaca aggagaggat agttgggctt tgccaggtgg
    61621 ctttggtgaa gtcggctatt ctccaactga aaatattctc aaggaaattg aagaagaaac
    61681 cggttttaaa gccaaagttg aaagactgct agctgttttt gataccaatc gtttccaact
    61741 acagagcaaa caatatgcaa agtttgtttt tgaatgtaag cttctcgatg gacaattcca
    61801 agaaaatcaa gaaatcgctg accttcaatt ttttgcaatt gacccactgc cgaatttatc
    61861 tgaaaaacgc attaccaagg agcaaatcga gattctttgg caggtttatc aaggtcagag
    61921 ggagcaatat cttgactaag aagatgatta tcgtatttct aaattcattt ttcttaagta
    61981 gcatggtata ataaaatgca ggaaaatttt gaatcatgag gaagactaga tgaatttatg
    62041 ggatattttc tttacgactc aggcaaccga gccgcccaaa tttgaccttt tttggtatgt
    62101 tagcctattt acgctcttag ccttaacctt ttatacagcc catcgctatc gtgaaaagaa
    62161 ggtttaccaa cgatttttcc aaatcttgca gactgttcag ttaatccttc tttatggttg
    62221 gtactgggtc aatcatatgc cactgtcaga aagcctaccc ttttaccatt gccgtatggc
    62281 tatgtttgtg gtactcttgc ttcctggtca gtccaaatat aaacaatact ttgcattatt
    62341 gggaacattt gggacattag cagcctttgt ttatccagtg ccagatgctt acccttttcc
    62401 acatatcacc attctatcct ttatctttgg tcatttagca ctcttgggga actctctagt
    62461 ttatctattg agacagtata atgcgcgatt gctggatgtg aagggaattt ttctcatgac
    62521 ctttgcccta aatgccttga tttttgtggt caatttggtg acaggtggcg attacggatt
    62581 tttgacaaaa ccgccattgg ttggggatca cggtctagta gctaattatt tacttgtttc
    62641 aattgtgctg gtagctacta tcagtttgac taagaaaatc ttagaattct ttttagctca
    62701 agaagcagaa aaaatgattg caaaggaagc ttaacacaga gctttctttt ttgctcttag
    62761 agagttttta caagcagctt ataaaataag aatttctgaa tagacaaact caaaaaatgg
    62821 ctgggaaatt taggaaaaaa gcaagcacga ttaaattttt tgtgttataa tattttgtga
    62881 atagctatgc ctatgtttag ctatggaata atacgaagtg cgaaacttgg aagatagaga
    62941 ggaagcgatg taatggctag agaaggcttt tttacaggtc tagatattgg aacaagctct
    63001 gtcaaggtgc ttgtggccga gcagagaaat ggtgaattaa atgtaattgg cgtgagtaat
    63061 gccaaaagta aaggtgtaaa ggatggaatt attgttgata ttgatgcagc agcaactgct
    63121 atcaagtcag ccatttccca agcggaagaa aaggcaggca tttcgattaa atcagtaaat
    63181 gtcggcttgc ctggtaatct tttgcaggta gaaccaactc aggggatgat tccagtaaca
    63241 tctgatacta aggaaattac ggatcaagat gttgaaaatg ttgtcaaatc agctttgaca
    63301 aagagtatga cacctgaccg tgaagtcatt acctttattc ctgaagaatt tattgtggat
    63361 ggtttccaag ggattcgtga cccacgtggc atgatggggg ttcgccttga aatgcgtggt
    63421 ttgctttata caggacctcg tactatcttg cacaatttgc gtaagacggt tgagcgtgca
    63481 ggtgttcagg ttgaaaatat tatcatttca ccactagcaa tggttcagtc tgttttgaat
    63541 gaaggggaac gtgaatttgg tgctacagtg attgatatgg gggcaggtca aacgactgtc
    63601 gctacaatcc gtaatcaaga actccagttc acacatattc tccaagaagg tggagattat
    63661 gtaactaaag atatctccaa ggttttgaaa acctctcgca aattagcgga aggcttgaaa
    63721 ctgaattacg gggaagccta tccgcctctt gcaagcaaag aaaccttcca agtagaggtt
    63781 attggagaag tggaagcagt cgaagtgacg gaagcctact tgtcagaaat tatttctgca
    63841 cgaatcaagc acatccttga acaaatcaag caagaattag atagaaggcg tctattggac
    63901 ctccctggtg gtattgtctt aatcggtggg aatgccattt taccaggtat ggttgagctt
    63961 gctcaggaag tctttggcgt ccgtgtcaag ctttatgttc caaatcaagt tggtatccgt
    64021 aatccagcct ttgcgcatgt gattagttta tcagaatttg cgggtcaatt aacagaagtt
    64081 aatcttttgg ctcagggagc gataaaaggt gagaatgact taagtcatca gccaattagt
    64141 tttggtggga tgctgcaaaa aacagctcag tttgtacaat caacgcctgt tcaaccagct
    64201 cctgctccag aagtagagtc ggtggcgcct acagaaccaa tggcggattt ccaacaagct
    64261 tcacaaaata aaccgaaatt agcagatcgt ttccgtggct tgatcggaag catgtttgac
    64321 gaataaagag gaaaaataaa ttatgacatt ttcatttgat acagctgctg ctcaaggggc
    64381 agtgattaaa gtaattggtg tcggtggagg tggtggcaat gccatcaacc gtatggtcga
    64441 cgaaggtgtt acaggcgtag aatttatcgc agcaaacaca gatgtacaag cattgagtag
    64501 tacaaaagct gagactgtta ttcagttggg acctaaattg actcgtggtt tgggtgcagg
    64561 aggtcaacct gaggttggtc gtaaagccgc tgaagaaagc gaagaaacac tgacggaagc
    64621 tattagtggt gccgatatgg tcttcatcac tgctggtatg ggaggaggct ctggaactgg
    64681 agctgctcct gttattgctc gtatcgccaa agatttaggt gcgcttacag ttggtgttgt
    64741 aacacgtccc tttggttttg aaggaagtaa gcgtggacaa tttgctgtag aaggaatcaa
    64801 tcaacttcgt gagcatgtag acactctatt gattatctca aacaacaatt tgcttgaaat
    64861 tgttgataag aaaacaccgc ttttggaggc tcttagcgaa gcggataacg ttcttcgtca
    64921 aggtgttcaa gggattaccg atttgattac caatccagga ttgattaacc ttgactttgc
    64981 cgatgtgaaa acggtaatgg caaacaaagg gaatgctctt atgggtattg gtatcggtag
    65041 tggagaagaa cgtgtggtag aagcggcacg taaggcaatc tattcaccac ttcttgaaac
    65101 aactattgac ggtgctgagg atgttatcgt caacgttact ggtggtcttg acttaacctt
    65161 gattgaggca gaagaggctt cacaaattgt gaaccaggca gcaggtcaag gagtgaacat
    65221 ctggctcggt acttcaattg atgaaagtat gcgtgatgaa attcgtgtaa cagttgttgc
    65281 aacgggtgtt cgtcaagacc gcgtagaaaa ggttgtggct ccacaagcta gatctgctac
    65341 taactaccgt gagacagtga aaccagctca ttcacatggc tttgatcgtc attttgatat
    65401 ggcagaaaca gttgaattgc caaaacaaaa tccacgtcgt ttggaaccaa ctcaggcatc
    65461 tgcttttggt gattgggatc ttcgccgtga atcgattgtt cgtacaacag attcagtcgt
    65521 ttctccagtc gagcgctttg aagccccaat ttcacaagat gaagatgaat tggatacacc
    65581 tccatttttc aaaaatcgtt aagtaaatga atgtaaaaga aaatacagaa cttgtttttc
    65641 gagaagttgc agaggctagt ctgagtgctc atcgagagag tggttcggtc tctgtcattg
    65701 cagttaccaa gtatgtagat gtaccgacag cggaagcctt gcttccgcta ggtgttcatc
    65761 atatcggtga aaatcgtgta gataagtttc tggaaaaata tgaagcttta aaagatcgag
    65821 atgtgacttg gcatttgatt ggtaccttgc aaagacgtaa ggtgaaagat gtcattcaat
    65881 acgttgatta tttccatgca ttggactcag taaagctagc aggggaaatt caaaaaagaa
    65941 gtgaccgagt catcaagtgt ttccttcaag taaatatttc taaagaagaa agcaaacacg
    66001 gtttttcgag agaggaactg ctggaaatct tgccagagtt agccagacta gataagattg
    66061 aatatgttgg tttaatgacg atggcacctt ttgaggctag cagtgagcag ttgaaagaga
    66121 ttttcaaggc gacccaagat ttacaaagag aaattcaaga gaaacaaatt ccaaatatgc
    66181 ctatgaccga gttaagtatg ggaatgagtc gtgattataa agaagcgatt caattcggtt
    66241 ccacttttgt tcgtataggt acatcatttt ttaagtagga gagaaccatg tctttaaaag
    66301 atagattcga tagatttata gattatttta cggaggatga ggattcaagt ctcccttatg
    66361 aaaaaagaga tgagcctgtg tttactccag taaattcttc acaggaaccg gctctcccaa
    66421 tgaatcaacc ttcacagtcg gctggtacaa aagagaacaa tatcaccaga cttcatgcaa
    66481 gacaacagga attggcaaat cagagtcagc gtgcaacgga taaggtcatt atagatgttc
    66541 gttatcctag aaaatatgag gatgcaacag aaattgttga tttattggca ggaaacgaaa
    66601 gtatcttgat tgattttcag tatatgacag aggtgcaggc tcgtcgttgt ttggactatt
    66661 tggatggagc ttgtcatgtt ttagctggaa atttgaaaaa ggtagcttct accatgtatt
    66721 tgttgacacc agtgaatgtt attgtaaatg ttgaagatat ccgtttacca gatgaagatc
    66781 aacagggtga gttcggtttt gatatgaagc gaaatagagt acgataatga tttttttaat
    66841 tcgtatgatt tataatgcag tggatattta ctccctgatt ttggtagcct tcgctgtcat
    66901 gtcttggttt ccaggtgcct acgaatccag tttaggtcgt tggattgtag cgttggtgaa
    66961 accagtgctt gctcccttgc aacgcctgcc tttacagata gcgggtcttg atttatctgt
    67021 ttgggtcgcg attgttttgg ttcgattttt aggagaaaac ctagtgcgtt ttctggcgat
    67081 gataggatga ataaagggat ttatcagcat ttctccatag aagatcgtcc atttcttgac
    67141 aagggaatgg aatggataaa gaaggtagaa gatagctatg ctcctttttt aactcctttt
    67201 atcaatcctc atcaggagaa gctattaaag attttggcca aaacctatgg tcttgcttgt
    67261 agcagtagtg gggaattcgt ctcgagtgag tatgttcgag ttttattata cccagattat
    67321 ttccaaccag agttttcaga ttttgaaata tctctccagg aaattgtgta ttccaataaa
    67381 tttgaacatt taacgcatgc taagatttta gggacagtca tcaatcaatt agggattgaa
    67441 cggaaacttt ttggagatat cctagtagat gaagaacggg cgcagattat gattaatcag
    67501 cagtttcttc ttctctttca agatggacta aagaaaattg gtcgtatacc tgtttcgctg
    67561 gaggaacgtc ctttcaccga gaaaatagat aagctagaac agtatcgaga actggattta
    67621 tctgtgtcta gttttcgatt agatgttctt tgttcaagtt ggagacttga ttagtgtgag
    67681 aaaatttggt cgcttgagat tacttcaaga taagggacaa acgaaaaaag agaagaaaaa
    67741 aataaccgtc cagttattat taagtaagtg aggaatagaa tgccaattac atcattagaa
    67801 ataaaggaca agacttttgg aactcgattc agaggttttg atccagaaga agtcgatgaa
    67861 tttttagata ttgtggttcg tgattacgaa gatcttgtgc gtgcgaatca tgataaaaat
    67921 ttgcgtatta agagtttaga agagcgtttg tcttactttg atgaaataaa agattcattg
    67981 agccagtctg tattgattgc tcaggataca gctgagagag tgaaacaggc ggcgcatgaa
    68041 cgttcaaaca atatcattca tcaagcagag caagatgcgc aacgcttgtt ggaagaagct
    68101 aaatataagg caaacgagat tcttcgtcaa gcaactgata atgctaagaa agtcgctgtt
    68161 gaaacagaag aattgaagaa caagagccgt gtcttccacc aacgtctcaa atctacaatt
    68221 gagagtcagt tggctattgt tgaatcttca gattgggaag atattctccg tccaacagct
    68281 acttatcttc aaaccagtga tgaagccttt aaagaagtgg ttagcgaagt acttggagaa
    68341 ccgattccag ctccaattga agaagaacca attgatatga cacgtcagtt ctctcaagca
    68401 gaaatggaag aattacaagc tcgtattgag gtagccgata aagaattgtc tgaatttgaa
    68461 gctcagatta aacaggaagt ggaaactcca actcctgtag tgagtcctca agttgaagaa
    68521 gagcctctgc tcatccagtt ggcccaatgt atgaagaacc agaagtagct ccagtgcatc
    68581 cgacaggtcc aacaccagct acagaaactg ttgattcagc accgggattt gaagcaccgc
    68641 aagaatctgt tacaatttta taagaaatat tctgagaaca atatcttatc cttatattcc
    68701 cagcgagcag gagatggtgt gagtcctgca ttccctatcg ataagattat cctctcaaac
    68761 tatcaagtct gaatctagta agatttgacg ttccccacgt tacgggataa gagggagaaa
    68821 gactaaaact ttttccgaat aaaggtggta ccacgatttt cgtccttttt ggaagtcgtg
    68881 gtttttaatt tgttattatt tataaaggag ataccatgaa actcaaagac acccttaatc
    68941 ttgggaaaac tgaattccca atgcgtgcag gccttcctac caaagagcca gtttggcaaa
    69001 aggaatggga agatgcaaaa ctttatcaac gtcgtcaaga attgaaccaa ggaaaacctc
    69061 atttcacctt gcatgatggc cctccatacg ctaacggaaa tatccacgtt ggacatgcta
    69121 tgaacaagat ttcaaaagat atcattgttc gttctaagtc tatgtcagga ttttacgcac
    69181 catttattcc tggttgggat actcatggtc tgccaatcga gcaagtcttg tcaaaacaag
    69241 gtgtcaaacg taaagaaatg gacttggttg agtacttgaa actttgccgt gagtacgctc
    69301 tttctcaagt agataaacaa cgtgaagatt ttaaacgttt gggtgtttct ggtgactggg
    69361 aaaatccata tgtgaccttg actcctgact atgaagcagc tcaaattcgt gtatttggtg
    69421 agatggctaa taagggttat atctaccgtg gtgctaagcc agtttactgg tcatggtcat
    69481 ctgagtcagc ccttgctgaa gcagagattg aataccatga cttggtttca acttcccttt
    69541 actatgccaa caaggtaaaa gatggcaaag gagttctaga tacagatact tatatcgttg
    69601 tctggacaac gactccattt actatcacag cctctcgtgg tttgacggtt ggtgcagata
    69661 ttgattacgt tttggttcaa cctgctggtg aagctcgtaa gtttgtcgtt gctgctgaat
    69721 tattgactag cttgtctgag aaatttggct gggctgatgt tcaagttttg gaaacttacc
    69781 gtggccaaga actcaaccac atcgtaacag aacacccatg ggatacagct gtagaagagt
    69841 tggtaattct tggtgaccac gttacgactg actctggtac aggtattgtc catacagccc
    69901 ctggttttgg tgaggacgat tacaatgttg gtattgctaa taatcttgaa gtcgcagtga
    69961 ctgttgatga acgtggtatc atgatgaaga atgctggtcc tgaatttgaa ggtcaattct
    70021 atgaaaaggt agttccaact gttattgaaa aacttggtaa cctccttctt gcccaagaag
    70081 aaatctctca ctcatatcca tttgactggc gtactaagaa accaatcatc tggcgtgcag
    70141 ttccacaatg gtttgcctca gtttctaaat tccgtcaaga aatcttggac gaaattgaaa
    70201 aagtgaaatt ccactcagaa tggggtaaag tccgtcttta caatatgatc cgtgaccgtg
    70261 gtgactgggt tatctctcgt caacgtgctt ggggtgttcc acttcctatc ttctacgctg
    70321 aagatggtac agctatcatg gtagctgaaa ctattgaaca cgtagctcaa ctttttgaag
    70381 aacatggttc aagcatttgg tgggaacgtg atgccaaaga cctcttgcca gaaggattta
    70441 ctcatccagg ttcaccaaac ggcgagttca aaaaagaaac tgatatcatg gacgtttggt
    70501 ttgactcagg ttcatcatgg aatggagtgg tggtaaaccg tcctgaattg acttacccag
    70561 ccgaccttta cctagaaggt tctgaccaat accgtggttg gtttaactca tcacttatca
    70621 catctgttgc caaccatggc gtagcacctt acaaacaaat cttgtcacaa ggttttgccc
    70681 ttgatggtaa aggtgagaag atgtctaaat ctcttggaaa taccattgct ccaagcgatg
    70741 ttgaaaaaca attcggtgct gaaatcttgc gtctctgggt aacaagtgtt gactcaagca
    70801 atgacgtgcg tatctctatg gatatcttga gccaagtttc tgaaacttac cgtaagattc
    70861 gtaacactct tcgtttcttg attgccaata catctgactt taacccagct caagatacag
    70921 tcgcttacga tgagcttcgt tcagttgata agtacatgac gattcgcttt aaccagcttg
    70981 tcaagaccat tcgtgatgct tacgcaaact ttgaattctt gacaatctac aaggccttgg
    71041 tgaactttat caacgttgac ttgtcagcct tctaccttga ttttgccaaa gatgttgttt
    71101 acattgaagg tgccaaatca ctggaacgcc gtcaaatgca gactgtcttc tatgacattc
    71161 ttgtcaaaat caccaaactc ttgactccaa tccttcctca cactgcggaa gaaatctggt
    71221 catatcttga gtttgaagcc gaagacttcg tccaattgtc agaattacca gaagttcaaa
    71281 cttttgctaa ccaagaagaa atcttggata catgggcagc cttcatggac ttccgtggac
    71341 aagcacaaaa agccttggaa gaagctcgta atgcaaaagt tatcggtaaa tcacttgaag
    71401 cacacttgac agtttatcca aatgaagttg tgaaaactct actcgaagca gtaaacagca
    71461 atgtagctca acttttgatc gtgtctgagt tgaccatcgc agaagaacca gctccggaag
    71521 ctgcccttag cttcgaagat gtagtcttca cagttgaacg cgctgcaggt gaagtatgtg
    71581 accgttgccg tcgtattgac ccaacaacag cagaacgtag ctaccacgca gtcatctgtg
    71641 accactgtgc aagtatcgta gaagaaaact ttgcagatgc agtcgcagaa ggatttgaag
    71701 agaaataaga ttgaaaagtc taggtaaaac tcaatttgag aagaaaagac aactaatttt
    71761 atagtctatt aaacgcattg tatcacgttt ttgaacacct gatatgatgc gttttttatt
    71821 tattttaaaa atttgcgagg tatgactttt tatactcaac aagaatcaaa gagaaactta
    71881 gcaagctaac agtagtaata taaaatagga atttgatatt agggataaga ttggtaaata
    71941 gtgtaatatt tttacaacaa taaatttata tagttatttc tggtttctga aaagtattat
    72001 attatatttt atttcatatt atacaaattt ttattttata atatcagaaa atactttttt
    72061 taaaaagcaa atatgataca attttatttg aaaaaaaata aaaaaggaga ttttattatg
    72121 aaatcaagtg gtatcgtcgg tcttgtggga ggaattttac ttcttatttg tccttttgtc
    72181 ttgttgggaa cagcggtaaa tacagctgct acaactctta atggaggagc tactgcaggg
    72241 gctttttcag gtgttgcctt actcttgaat gccttgaaga ttgcaaatct tgttcttggt
    72301 atcattgcta ttgtttacta taaaggagat aagcatgtag gtgcagctcc gtctgtacta
    72361 atgattgttt ctggtgaagt tagtctcatt ccattcttag gatgggttgg ggggattctt
    72421 gctattatcg gaggatctct attccttgca acattgaaga aattcaaatc agaagaataa
    72481 aaggtatttt agcatgaaaa gaacaaaaaa gtttatcggt ataggagtag ctctattatc
    72541 tctttctctt ctagttgcat gtggaacata aagttcaaag aatacttcaa caagtaatga
    72601 tgagaagaca gtagcaacat ccaatagttc aaaagaaaca atcactttcg atacaccggt
    72661 tgtaacagac gatgcgattg aatcaatacg cacttatgca gattatatag atctttataa
    72721 aaatattttt gatgattatt ttactaaagc tgaggaaggt ttcaaaggca caactatgga
    72781 aaataatgac tcgtttacta aactaaaaga gtcaactcaa aaattattcg atgcgcagaa
    72841 aaaaaggtta aataatgaag atagaataga aacaaccaaa aacaatgtga ttgccaaaca
    72901 ttgtcaaaca gtcctttcct ttttggtttt gactagcttt tttgtgaaaa attgtgtaaa
    72961 atagaataga taaacgaggg gaaacctcgg aaaatttaaa ggagaatcca tctaatggta
    73021 aaattggttt ttgctcgcca cggtgagtct gaatggaaca aagctaacct tttcactggt
    73081 tgggctgatg ttgatttgtc tgaaaaaggt acacaacaag cgattgacgc tggtaaattg
    73141 atcaaagaag ctggtatcga atttgaccaa gcttacactt cagtattgaa acgtgctatc
    73201 aaaacaacta acttggctct tgaagcttct gaccaattgt gggttccagt tgaaaaatca
    73261 tggcgcttga acgaacgtca ctacggtggt ttgactggta aaaacaaagc tgaagctgct
    73321 gaacaatttg gtgatgagca agttcacatc tggcgtcgtt catacgatgt attgcctcca
    73381 aatatggacc gtgatgatga gcactcagct cacacagacc gtcgttatgc ttcacttgac
    73441 gactcagtca ttccagatgc tgaaaacttg aaagtgactt tggaacgtgc tcttccattc
    73501 tgggaagata aaatcgctcc agctcttaaa gatggtaaaa acgtattcgt aggagctcac
    73561 ggtaactcaa tccgtgccct tgtaaaacac atcaaaggtt tgtcagatga tgagatcatg
    73621 gacgtggaaa tccctaactt cccaccattg gtattcgaat tcgacgaaaa attgaacgtt
    73681 gtttctgaat actaccttgg aaaataaaaa attgtaagcc taggattgat ttctaggctt
    73741 tttatgttag tatggaagta tgataaggaa taaaaaacaa gattatgtac tggcctacaa
    73801 gcaaccagct tcaaccactt acatgggttg ggaagaagaa gctttaccga taggcaatgg
    73861 ttctttagga gcaaaagtat ttggccttat aggggctgaa cggattcaat ttaatgaaaa
    73921 aagtctctgg tctggaggtc cacttcctga tagttcagat tatcagggtg gaaatcttca
    73981 ggatcagtat gtttttttag ctgagattcg gcaggctttg gagaagagag attacaatct
    74041 ggctaaggaa ctggctgagc agcacctaat tgggccaaaa acgagtcaat atgggaccta
    74101 tctgtctttt ggggatattc acattgagtt cagccagcaa ggtacgactt tgtctcaggt
    74161 gacggactat cagagacagc tgaatattag taaggcactt gcgacgactt cttatgtcta
    74221 taagggaacg cgatttgaac gtaaagcttt tgcgagtttt ccagatgatc tcttgattca
    74281 atgttttact aaggaagggt tggaaactct agattttact atagaactat ccttgacctg
    74341 tgatttggct tctgatggaa agtatgagca ggaaaaatct gattacaagg agtgtaagtt
    74401 ggatattact gattctcata tcttgatgaa gggaagagtt aaggataatg atctgcggtt
    74461 tgctagttat ctagcttggg aaacggatgg agatattaga gtttggtcag atagggttca
    74521 gatatcagga gccagttatg ccaatctctt cttggccgct aagacggatt ttgcccaaaa
    74581 tcctgctagc aattatcgca agaaactaga tttagagcaa caggtgatag acttggtgga
    74641 cacagctaaa gaaaagggct atacccaatt gaaatcaagg catatcgagg actaccaagc
    74701 cttattccag cgtgttcaat tggatttgga agctgatgtt gacgcatcca ctacagatga
    74761 tttgttaaaa aattataagc cacaagaagg gcaggctttg gaggagctgt tcttccagta
    74821 tggacggtat ttattgatta gttcgtccag agactgccca gatgctctac cagctaacct
    74881 acagggagtc tggaatgcgg tcgacaatcc tccttggaat tcggactatc acttaaatgt
    74941 caatctgcag atgaattatt ggccagccta tgttaccaat ctcctagaga cggtctttcc
    75001 agtcatcaac tatgtagatg atttgcgtgt ctatggtcgt ctagcggctg taaagtatgc
    75061 aggaatcgtc tctcaaaaag gtgaggaaaa tggttggttg gttcatactc aagcgactcc
    75121 ctttggttgg acggcacctg gttgggatta ctattggggt tggtcaccag ctgccaatgc
    75181 gtggatgatg caaaccgttt atgaagccta ttcattttat agggaccaag actatctcag
    75241 ggagaaaatt tatcccatgt tgagggaaac ggttcgtttt tggaatgcct ttttacataa
    75301 ggatcagcag gcgcagcgtt gggtctcttc tccgtcttat tccccagaac atgggccgat
    75361 ttcgattggc aatacctatg accaatctct gatttggcag ttatttcatg attttattca
    75421 ggctgctcag gaattgggac tggatgagga cttgttgact gaggttaagg agaagtctga
    75481 tttactaaat cctttgcaaa tcactcaatc tggtcgaatc agggagtggt atgaggagga
    75541 agagcagtat tttcaaaatg agaaagtgga ggcccagcat cggcacgctt cccatctagt
    75601 gggactctat tctggcaatc tctttagcta caagggacaa gagtatattg aagcggcgcg
    75661 tgctatcctc aatgatcgtg gagatggcgg cacaggctgg tccaaggcta ataagatcaa
    75721 tctctgggcg cgtttgggag atggcaatcg agcccataaa ttattggcag agcagttaaa
    75781 gacatccacc ttgccaaatc tttggtgtag ccatcctcct tttcagatag atggtaattt
    75841 tggtgctact agtggcatgg cagaaatgtt actccagtct catgcagctt atctggtacc
    75901 tctagctgcc ctacctgatg cttggtcaac aggttctgtt tcaggcttaa tggcacgtgg
    75961 acattttgaa gtgagcatga gctgggaaga taaaaaactc ttacagttga ccattttatc
    76021 aaggagtgga ggagatttgc gagtttctta tccagatatt gagaagagtg tgattaaaat
    76081 gaatcaagaa aaaataaaag cgaaatgcat ggggaaagat tgtatttcgg tggcaacagc
    76141 agaaggtgat cttgttcaat tttattttta agaagatgtt ataaggcagt aatttgaaac
    76201 ttccttttaa taaggatttg agaatatagg cagtttccaa ctagttgaaa aagcgttata
    76261 atgataatag gaagtaatac tcaatgaaaa tcaaagagca caaactagga agctagccgc
    76321 aggttgctca aaacagtgtt ttgaggttgt agatggaagc tgacgtggtt tgaagagaga
    76381 ttttcgagga gtataatttg tttgatagag ggtgggtctg atggcttata ttgagatgaa
    76441 acactgttac aagcgttatc aggttgggga cacggagatt gtggccaatc gtgatgtgaa
    76501 ttttgagatt gaaaaggggg agctggttat tatccttggt gcttcaggtg caggcaagtc
    76561 aacagttctt aaccttcttg ggggaatgga taccaatgat gaaggggaaa tctggattga
    76621 tggtgttaat attgcggatt atagttccca ccagcgtacc aattaccgta gaaatgatgt
    76681 ggggtttgtt tttcagtttt ataatctagt ttctaatctg acagctaagg aaaatgtgga
    76741 actggcttct gaaattgtga cagatgcctt gaatcctgat caggtcttga cagatgtagg
    76801 tctggctcat cgtctcaata actttccagc ccagctttct ggaggggagc aacagcgagt
    76861 ctccattgca cgcgcggtag ccaaaaatcc taaaattctc ctttgtgatg aaccgactgg
    76921 agccttggat tatcagacgg gcaagcaggt tttgaaaatt ctccaagaca tgtctcgtca
    76981 aaagggagcg acggtgatca tcgtgactca taatggagct ttggcgccca ttgctgatcg
    77041 cgtgattcaa atgcacgatg ccagtgtcaa ggatgtggtg ctcaaccagc atcctcagga
    77101 tattgacagt ttggagtact agcatgatca agcgaaaaac ttattggaag gacttaattc
    77161 agtccttcac aggctccaag gggcgttttt tatccatctt gatcctgatg atgttgggat
    77221 ctctagcctt agtaggcctc aaagtaacca gtcccaacat ggaggcgaca gctaatgctt
    77281 atttaacaac tgctcaaacc ttggatttgg cagtcatgtc taactatggc ttggatcaag
    77341 cagaccaaga agaactaaaa cagacggagg gcgcagaggt cgagtttggc tatttgacag
    77401 atgtgactat ggataatggg caggatgcca ttcggctgta ctccaaacca gagcgaattt
    77461 caacctttca gctaagaaag ggacgacttc ctcagtcaga caaggaaatc gctttggcca
    77521 ctcatttgca aggccaatac agcgtgggac aggagattag ttttaaagaa aaagaagagg
    77581 gtcattcctc tttaaaagac catacttata ccattactgg ttttgtggat tcggctgaaa
    77641 tcctctccca gcgagatatg ggctacgcag gaagtggaag tgggactctg acagcctatg
    77701 gggtgatttt acctagtcag tttgatcaga aagtctacaa tatagctcgt ttgaaatatc
    77761 aagatttagc aggtttaaat gccttttcat cagcttatga agaaaaatcc aagcaacatc
    77821 aggaagacct tgaacaaact ttatcagata atggcaaggt acgtctgcaa cttttgaaaa
    77881 aagaaggaca agagtctcta gacaaggggc aagagaccct tgacaaggct cagactaatt
    77941 tgcaggaagg caagcgtcgt ttagcagctg ctcaagctcg tatacaggct caagaaagtc
    78001 aactagcctt gtttcctcaa gttcagagag agcaggctag tgctcaactt acccaagcca
    78061 agcaggaatt gggcaaggaa gaggataaac taaagcaagc tgaacaaaat ctagcccaag
    78121 aaaaggaaaa attagaaaaa catcagcaag tcttggacga tttggcggag ccaaggtatc
    78181 aggtctataa tcgtcagacc atgccaggtg gtcagggcta tcttatgtat agcaatgctt
    78241 catccagtat tcgagcagtg ggcaatatct ttcctgtggt actttatgcc gtagcatcca
    78301 tggtgacctt tacgaccatg actcgctttg tagacgaaga gcgaactcat gcagggattt
    78361 ttaaggcctt gggttaccgt agtaaggata ttatcaccaa gtttctcctt tatggactag
    78421 tagctgggac tgtcggaacg gctctaggta gtatacttgg tcattatttg ctagccagtg
    78481 taatttcaag tgtcattaca aaaggcatgg tggtgggaga aacccagatt cagttctatt
    78541 ggacctatag cttactagct cttgtcttga gctggttggc gagtgtgtta ccagcctatc
    78601 tggtggcttg gagggaactt catgccgaag cagcccagct tctacttcct aaacctcctg
    78661 tcaaaggagc taaaatctta ttggagcgta tcggttttat ctggcgtcgt ctcagtttta
    78721 ctcataaggt aacagcccgc aacatctttc gttataagca gagaatgttg atgacaatct
    78781 ttggtgtggc aggttctgta gctctgctct ttgcaggttt gggaatccaa tcttctgtag
    78841 caggagttcc gtctaaacag tttcaacaaa tccaacagta tcagatgctt gtctctgaaa
    78901 atcctagtgc gaccaatcag gacaaggtag agctagcaga agtgttgaaa gggcaggaga
    78961 tactagccta ccagaaaatc tattctaaaa cgctagacaa ggatttcaaa ggcaaggctg
    79021 gtcttcaaaa cattactctt atgatgatag agaaggaaga tttgactccc tttatccatc
    79081 ttcaacatca tcagcaggag ctgacattaa aagatggcat cgttattaca gctaaactcg
    79141 cccagctggc aggtgtcaag gttgggcaga ctttagaaat tgaaggtaag gaactaaagg
    79201 tcgctgctat tactgagaac tacgttggtc actttattta tatgagtcag gctagctatg
    79261 agcaacttta cggacagcta ccccaagcca acacttatct ggtctcatta agggatacca
    79321 gtgcaactag tatcgaaagt caggcgggct tgcttatgaa tcaatctgcg gtgtccagcg
    79381 ttgtccaaaa tgcttcagcc attcgactct atcgctagct cactcaatca gaccatgacc
    79441 atcttggtca tcgtatcagt tctattagct attgtcatcc tttacaatct gaccaatatc
    79501 aacgtagctg agagaatccg tgaactctcc actatcaagg ttctcggttt tcataataat
    79561 gaagtcaccc tctacattta ccgtgagacg attgtgctgt cccttgtggg aatcgtactt
    79621 ggtctggtag ctggtttcta tttacaccaa tttttgattc aaatgatttc gcctgcgact
    79681 attctctttt atccgcaggt aggctgggaa gtctatgtaa tcccagtggc aacagtaagc
    79741 atcattttga ccttgcttgg tttcttcgtc aattattatc tgagaaaggt tgatatgtta
    79801 gaagccctga aatctgtaga gtaaggtagt tatttttagc tgattgaact tctatttact
    79861 aatattcaaa aatcctctgt cacaaagagc agggagctct ttgtgacaga ggattttttc
    79921 tatagggctt tagcagctgc aattgcggct tcgaagtttg gctcagaatt gatattatcc
    79981 acgtattcaa cgtagcgaat cgtattgtca gtatcgagga caaagactgc gcgtgctaat
    80041 aggtgccatt cgttgatcaa gagggcataa tcgcgcccga aagaatggtc aaagtagtct
    80101 gaaagcataa tggcattgtc aaggccttca gcaccgcacc aacgtttttg agcaaaaggt
    80161 aggtccattg aaacagtcaa tacgaccgtg ttgtccagtc cagccaattc ttcattaaaa
    80221 cgacgtgttt gagttgagca gatgcctgta tcgatagaag gaacgacact caagactttt
    80281 ttcttgccat caaaatcagc cagagatttt ttagaaagat ctgttgtagt aagagaaaaa
    80341 tcaagcgcct tgtcgccgac ttgtagttgt ttacctgtaa agctcacagg atttccgaga
    80401 aaagttacca taggatactc caatcttttt tcttccattg tatctgaaac agtcagaatt
    80461 ttccaatgat ttgaccggaa atgtgggcat agaaaaaacg ccagctcaca tgagaatgac
    80521 gtttttcaga ggcttatttt gccaatcctt cagcaatctt gtcaaggttg tatttcatca
    80581 tgctgtagta gctgtcgcct tctttacctt gttctgcgat agagtcagta aagatttgtg
    80641 cgtagattgg gatgtttgtg tcttgagaaa cagttttcat tggacggtca tccacacttg
    80701 attctacaaa gagtgatgga acttttgttt ggcgaagttt ttcaaccaag gtcttgattt
    80761 gttcaggagt tccttcttct tcagtattga tttcccagat gtaagcactt gggacaccat
    80821 aggctttaga gaagtatttg aatgctcctt cgctggttac aatgagtttc ttttcagcag
    80881 ggatcttatt aaatttatcc ttactttctt tatcaagttt gtctaactta tcagtatatt
    80941 ctttgagatt tttttcatag aattctttat tgttagggtc tttggcgctc aattgtttgg
    81001 cgatattttt agcaaaaata ataccgtttt caaggttaag ccaagcgtgt gggtcttctt
    81061 ttcctttttc attttgacct tcaaggtaga taacatcaac gccgtcgctg actgcgaagt
    81121 agtctttgtt ttcagttttc ttggcatttt ctaccaattt tgtaaaccaa gcattgccac
    81181 ctgtttcaag gttgataccg ttatagaaaa tcaaatcagc ctcagaagtt ttcttaacgt
    81241 cttcaggaag tggttcgtat tcgtgtgggt cttgcccaat cggaacgata ctatgaaggt
    81301 caattttgtc accagcaata tttttagtaa tatcagcgat gattgagttt gtagcaacaa
    81361 cttttagttt ttgaccagaa gttgtatctt tttttccgct agcacatgct acaagaatga
    81421 ttgcagaaag aaagagaacg agtaatgtac ctaatttttt cattagatcc tccaaattat
    81481 tagggctttg ccccttattt taacaaatgt ttatttttca gtttcaaata tcgttgtttg
    81541 ggagcgataa agaagctaat gagaaagaaa ctagcagctg taagcacgat actagaacct
    81601 gccgcaacat taaaactata gccaataaag agtcccaaaa ctgaagcagt agctccgaag
    81661 gttgaggaaa ggaaaatcat gcttttcaga ctattagcat acagataagc agttgcagct
    81721 ggggtaatca gcatggctac aatcaggata gttccgacac tttgcatggc tgtcacagac
    81781 acgagagtca ggagtaccat gagaaggtag tgatagaaat tgacaggcat tcccatggct
    81841 ttagccaaga gttcatcaaa ggaagttatc aagagttgct tgaagaaaat ccagattaac
    81901 aagaggatgg ctgcccccac acccatagta ataaacatat ccgtatcttg gacggccagg
    81961 atattaccaa aaaggatatg gaaaaggtca gttgaacttt tagcgacacc aatcaagatg
    82021 ataccgaggg ctaagaaaga agaaaaggta atgccgatgg cggtatcgct tttgataatc
    82081 gagtttcctt tgatgtaggt aatgatgatg gcagctagca atccaaagac aatggctccg
    82141 ataaagaagt caaggcccaa gatgaaggag agggctacac ctggtaagac agcatgtgaa
    82201 atggcatctc ccatgagtga catcccgcgt agaataatga aacatcccac agctccagct
    82261 acaatcccga cgacaatagc tgttatcaag gcattttgta ggaaatggaa tttttgcaat
    82321 ccatcgataa attctgcaat cataggtcac ctccattgaa aaagagttga ttaccgtaag
    82381 cttcttttag attggtttcg gtaaaagttt cttttgttgg accaaaggca atcacttctc
    82441 gattgacaag taagacttga tcgaagtagt ggggaatctt gctgaggtcg tggtgaacga
    82501 tgagaaccgt cttcccagct tttttcaaat ctctcagcgt attcatgatg atttcctcac
    82561 tgacagagtc aatcccagca aagggttcat ccaagaggat atagtcggct tcctgcacca
    82621 aacatctggc aatcaaaacc cgctggaatt gacctccaga cagttgacta atttgacgtt
    82681 cagcgtagtc agctaggccg acgatttcaa gggcctcttg cactttcttc caatgtttag
    82741 cctttaaact tcgaaagaga ggaatagagg gaaatagtcc taacgagacg cattccttga
    82801 ccttgatggg aaagttgtag tcgatattga ttttttgttc gacataggca attcggtgta
    82861 aggatttttt aacttccttg tcatcgagaa atgcctgacc ttgatgtggg ataattccca
    82921 gcataccttt taatagtgtt gatttcccag cgccgtttgg accaatgatg ccggtaattg
    82981 ttggtccatg gagcactagt gaaatatcct taagtgccaa cgtttctttg taggagacac
    83041 tgaggttttc gatacgtatc ataaacttgt attcctcctg tctcttaata tacattaaaa
    83101 aaaaaattaa gtcaagttaa tttttgaaaa aattaaaata ataactgaaa aataga
//
LOCUS       DAVBBS010000002        80223 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVBBS010000002 DAVBBS010000000
VERSION     DAVBBS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 80223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80223
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.002"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(134..1882)
                     /locus_tag="VQU32_000076"
     CDS             complement(134..1882)
                     /locus_tag="VQU32_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2471612.1"
                     /translation="MKRQTVNQTLKRLVVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKELSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTISRVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            complement(1884..3608)
                     /locus_tag="VQU32_000077"
     CDS             complement(1884..3608)
                     /locus_tag="VQU32_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2471613.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFLSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENLPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            complement(3666..4604)
                     /gene="msrB"
                     /locus_tag="VQU32_000078"
     CDS             complement(3666..4604)
                     /gene="msrB"
                     /locus_tag="VQU32_000078"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET2471614.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            complement(4746..4823)
                     /locus_tag="VQU32_000079"
                     /pseudo
     CDS             complement(4746..4823)
                     /locus_tag="VQU32_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4899..5768)
                     /locus_tag="VQU32_000080"
     CDS             complement(4899..5768)
                     /locus_tag="VQU32_000080"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET2471615.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTKGVRVEAK"
     gene            complement(5770..7056)
                     /locus_tag="VQU32_000081"
     CDS             complement(5770..7056)
                     /locus_tag="VQU32_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET2471616.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            complement(7207..7944)
                     /gene="mecA"
                     /locus_tag="VQU32_000082"
     CDS             complement(7207..7944)
                     /gene="mecA"
                     /locus_tag="VQU32_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HET2471617.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            complement(8144..9178)
                     /locus_tag="VQU32_000083"
     CDS             complement(8144..9178)
                     /locus_tag="VQU32_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET2471618.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            complement(9207..10640)
                     /locus_tag="VQU32_000084"
     CDS             complement(9207..10640)
                     /locus_tag="VQU32_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HET2471619.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFIAKMLTKEN"
     gene            complement(10656..11642)
                     /locus_tag="VQU32_000085"
     CDS             complement(10656..11642)
                     /locus_tag="VQU32_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2471620.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQIYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            complement(11644..12747)
                     /locus_tag="VQU32_000086"
     CDS             complement(11644..12747)
                     /locus_tag="VQU32_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET2471621.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDLEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQIIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            complement(12737..13582)
                     /locus_tag="VQU32_000087"
     CDS             complement(12737..13582)
                     /locus_tag="VQU32_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2471622.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLTLDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            complement(13861..15135)
                     /gene="psr"
                     /locus_tag="VQU32_000088"
     CDS             complement(13861..15135)
                     /gene="psr"
                     /locus_tag="VQU32_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HET2471623.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            complement(15132..15980)
                     /gene="pheA"
                     /locus_tag="VQU32_000089"
     CDS             complement(15132..15980)
                     /gene="pheA"
                     /locus_tag="VQU32_000089"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET2471624.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYIDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            complement(15977..16453)
                     /locus_tag="VQU32_000090"
     CDS             complement(15977..16453)
                     /locus_tag="VQU32_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET2471625.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRHVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            complement(16446..17729)
                     /gene="aroA"
                     /locus_tag="VQU32_000091"
     CDS             complement(16446..17729)
                     /gene="aroA"
                     /locus_tag="VQU32_000091"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET2471626.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            complement(17821..18159)
                     /locus_tag="VQU32_000092"
     CDS             complement(17821..18159)
                     /locus_tag="VQU32_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HET2471627.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            complement(18170..19273)
                     /locus_tag="VQU32_000093"
     CDS             complement(18170..19273)
                     /locus_tag="VQU32_000093"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HET2471628.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            complement(19283..20449)
                     /gene="aroC"
                     /locus_tag="VQU32_000094"
     CDS             complement(19283..20449)
                     /gene="aroC"
                     /locus_tag="VQU32_000094"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET2471629.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            complement(20459..21526)
                     /locus_tag="VQU32_000095"
     CDS             complement(20459..21526)
                     /locus_tag="VQU32_000095"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET2471630.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWKVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            complement(21545..22399)
                     /locus_tag="VQU32_000096"
     CDS             complement(21545..22399)
                     /locus_tag="VQU32_000096"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET2471631.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKKMPTEEIW
                     QSLTEKYQ"
     gene            complement(22389..23066)
                     /locus_tag="VQU32_000097"
     CDS             complement(22389..23066)
                     /locus_tag="VQU32_000097"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HET2471632.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            complement(23063..24226)
                     /locus_tag="VQU32_000098"
     CDS             complement(23063..24226)
                     /locus_tag="VQU32_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET2471633.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDERRFIVMDVFEYF
                     KYAKRKNLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            complement(24911..26545)
                     /locus_tag="VQU32_000099"
                     /pseudo
     CDS             complement(24911..26545)
                     /locus_tag="VQU32_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26526..27275)
                     /locus_tag="VQU32_000100"
     CDS             complement(26526..27275)
                     /locus_tag="VQU32_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2471634.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSAPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVEN
                     LRKDHPDQFLESIYLSLAGRKEEVADASQGH"
     gene            27454..28908
                     /locus_tag="VQU32_000101"
     CDS             27454..28908
                     /locus_tag="VQU32_000101"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HET2471635.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKEQGIQPMADVVL
                     NHKAAADNREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(28998..31616)
                     /gene="alaS"
                     /locus_tag="VQU32_000102"
     CDS             complement(28998..31616)
                     /gene="alaS"
                     /locus_tag="VQU32_000102"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET2471636.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLISPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNTSQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKIVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(31638..32123)
                     /locus_tag="VQU32_000103"
     CDS             complement(31638..32123)
                     /locus_tag="VQU32_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HET2471637.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLLDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(32398..32637)
                     /locus_tag="VQU32_000104"
     CDS             complement(32398..32637)
                     /locus_tag="VQU32_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471638.1"
                     /translation="MKMSTFFNKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILEK"
     gene            complement(32890..33960)
                     /locus_tag="VQU32_000105"
     CDS             complement(32890..33960)
                     /locus_tag="VQU32_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2471639.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDS
                     AYAFINFMLKPENALQNAEYVGYSTPNIPAKELLPEETKEDKAFYPDVETMKHLEIYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(33957..34730)
                     /locus_tag="VQU32_000106"
     CDS             complement(33957..34730)
                     /locus_tag="VQU32_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2471640.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(34727..35533)
                     /locus_tag="VQU32_000107"
     CDS             complement(34727..35533)
                     /locus_tag="VQU32_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2471641.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(35514..36671)
                     /locus_tag="VQU32_000108"
     CDS             complement(35514..36671)
                     /locus_tag="VQU32_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2471642.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(36730..37680)
                     /gene="murB"
                     /locus_tag="VQU32_000109"
     CDS             complement(36730..37680)
                     /gene="murB"
                     /locus_tag="VQU32_000109"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET2471643.1"
                     /translation="MSVREKMLEILEGIDIRFQEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR"
     gene            complement(37792..38544)
                     /locus_tag="VQU32_000110"
     CDS             complement(37792..38544)
                     /locus_tag="VQU32_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HET2471644.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWFEKKR
                     SLEKADALAYGLGHGGLELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLTAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(38554..39285)
                     /gene="budA"
                     /locus_tag="VQU32_000111"
     CDS             complement(38554..39285)
                     /gene="budA"
                     /locus_tag="VQU32_000111"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HET2471645.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFLHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            39427..40698
                     /locus_tag="VQU32_000112"
     CDS             39427..40698
                     /locus_tag="VQU32_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET2471646.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(40718..41533)
                     /locus_tag="VQU32_000113"
     CDS             complement(40718..41533)
                     /locus_tag="VQU32_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET2471647.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEILSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(41690..42343)
                     /gene="phoU"
                     /locus_tag="VQU32_000114"
     CDS             complement(41690..42343)
                     /gene="phoU"
                     /locus_tag="VQU32_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET2471648.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(42355..43113)
                     /gene="pstB"
                     /locus_tag="VQU32_000115"
     CDS             complement(42355..43113)
                     /gene="pstB"
                     /locus_tag="VQU32_000115"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2471649.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(43126..43929)
                     /gene="pstB"
                     /locus_tag="VQU32_000116"
     CDS             complement(43126..43929)
                     /gene="pstB"
                     /locus_tag="VQU32_000116"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2471650.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(43940..44824)
                     /gene="pstA"
                     /locus_tag="VQU32_000117"
     CDS             complement(43940..44824)
                     /gene="pstA"
                     /locus_tag="VQU32_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET2471651.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(44814..45731)
                     /gene="pstC"
                     /locus_tag="VQU32_000118"
     CDS             complement(44814..45731)
                     /gene="pstC"
                     /locus_tag="VQU32_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET2471652.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(45731..46609)
                     /gene="pstS"
                     /locus_tag="VQU32_000119"
     CDS             complement(45731..46609)
                     /gene="pstS"
                     /locus_tag="VQU32_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET2471653.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(47183..48487)
                     /locus_tag="VQU32_000120"
     CDS             complement(47183..48487)
                     /locus_tag="VQU32_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HET2471654.1"
                     /translation="MKFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEEFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(48488..49261)
                     /locus_tag="VQU32_000121"
     CDS             complement(48488..49261)
                     /locus_tag="VQU32_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET2471655.1"
                     /translation="MKSKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLASFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(49251..49529)
                     /locus_tag="VQU32_000122"
     CDS             complement(49251..49529)
                     /locus_tag="VQU32_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HET2471656.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGKGKISLEK"
     gene            complement(49529..49930)
                     /locus_tag="VQU32_000123"
     CDS             complement(49529..49930)
                     /locus_tag="VQU32_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HET2471657.1"
                     /translation="MITLFLSPSCTSCRKAKTWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(50140..50757)
                     /locus_tag="VQU32_000124"
     CDS             complement(50140..50757)
                     /locus_tag="VQU32_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HET2471658.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            complement(50747..51520)
                     /locus_tag="VQU32_000125"
     CDS             complement(50747..51520)
                     /locus_tag="VQU32_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HET2471659.1"
                     /translation="MKYKGYLIDLDGTIYKGKDQIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(51531..52268)
                     /locus_tag="VQU32_000126"
     CDS             complement(51531..52268)
                     /locus_tag="VQU32_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HET2471660.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIVEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEESISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            complement(52273..53403)
                     /locus_tag="VQU32_000127"
     CDS             complement(52273..53403)
                     /locus_tag="VQU32_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HET2471661.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(53487..53876)
                     /locus_tag="VQU32_000128"
     CDS             complement(53487..53876)
                     /locus_tag="VQU32_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET2471662.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(53892..54275)
                     /locus_tag="VQU32_000129"
     CDS             complement(53892..54275)
                     /locus_tag="VQU32_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HET2471663.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(54276..55064)
                     /locus_tag="VQU32_000130"
     CDS             complement(54276..55064)
                     /locus_tag="VQU32_000130"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET2471664.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(55057..55992)
                     /locus_tag="VQU32_000131"
     CDS             complement(55057..55992)
                     /locus_tag="VQU32_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HET2471665.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLSETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(56122..56298)
                     /locus_tag="VQU32_000132"
     CDS             complement(56122..56298)
                     /locus_tag="VQU32_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET2471666.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(56438..57145)
                     /locus_tag="VQU32_000133"
     CDS             complement(56438..57145)
                     /locus_tag="VQU32_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HET2471667.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            57296..58324
                     /gene="queA"
                     /locus_tag="VQU32_000134"
     CDS             57296..58324
                     /gene="queA"
                     /locus_tag="VQU32_000134"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET2471668.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIINMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDSMFIY"
     gene            complement(58995..59384)
                     /locus_tag="VQU32_000135"
     CDS             complement(58995..59384)
                     /locus_tag="VQU32_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2471669.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(59438..60145)
                     /locus_tag="VQU32_000136"
     CDS             complement(59438..60145)
                     /locus_tag="VQU32_000136"
                     /inference="COORDINATES: protein motif:HMM:NF019142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2471670.1"
                     /translation="MFRRNLNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGGIIVK
                     VATKPKVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGHVTE
                     QVTVDRKQATDTVIKVPAKSKVEEVLVPCATKYEADNDLSAGQEQEITLGKNGKTVTT
                     ITYDVDGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDDPTLDKSQE
                     VEEVGEIGKLLLLQSIL"
     gene            complement(60845..61396)
                     /locus_tag="VQU32_000137"
     CDS             complement(60845..61396)
                     /locus_tag="VQU32_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2471671.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDIEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(61794..62618)
                     /gene="nadE"
                     /locus_tag="VQU32_000138"
     CDS             complement(61794..62618)
                     /gene="nadE"
                     /locus_tag="VQU32_000138"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HET2471672.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(62615..64075)
                     /locus_tag="VQU32_000139"
     CDS             complement(62615..64075)
                     /locus_tag="VQU32_000139"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET2471673.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(64191..64679)
                     /locus_tag="VQU32_000140"
     CDS             complement(64191..64679)
                     /locus_tag="VQU32_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471674.1"
                     /translation="MKNRFYYYQLLDEREEQLINKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(64669..64878)
                     /locus_tag="VQU32_000141"
     CDS             complement(64669..64878)
                     /locus_tag="VQU32_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2471675.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            complement(65268..65710)
                     /locus_tag="VQU32_000142"
                     /pseudo
     CDS             complement(65268..65710)
                     /locus_tag="VQU32_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640911.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(66002..66808)
                     /locus_tag="VQU32_000143"
     CDS             complement(66002..66808)
                     /locus_tag="VQU32_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2471676.1"
                     /translation="MILKDINFALNKGEIVGLVGRNGVGKSTLMKILVQNNQPTSGNI
                     ISSDNVGYLIEEPKLFLSKTGLENLKYLSNLYGVDYNQERFRSLIQELDLTQSINKKV
                     KTYSLGTKQKLALLLTLVTEPDILILDEPTNGLDIESSQIVLAVLKKLALNENVGILI
                     SSHKLEDIEEICERVLFLENGLLTFQKVGKDSHNFLFEIAFSSATDRDIFITKQEFGD
                     IVQEEGLRITMSGNIQSSELFKFFNENSIKVVDFETKKETLKDIYLNRSK"
     gene            complement(67214..67942)
                     /locus_tag="VQU32_000144"
                     /pseudo
     CDS             complement(67214..67942)
                     /locus_tag="VQU32_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            complement(68178..68489)
                     /locus_tag="VQU32_000145"
     CDS             complement(68178..68489)
                     /locus_tag="VQU32_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471677.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYVCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(68504..69790)
                     /locus_tag="VQU32_000146"
     CDS             complement(68504..69790)
                     /locus_tag="VQU32_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2471678.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMKELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     KGEILEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESSEKFEAIKQNLVDEMWKVAQRELATGFYYGTPSENEQLFGARRKIPEYKFVAEVV
                     SYDDATQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQAGDMVRALKEGLINLYKEDGTSVTVRA"
     gene            70414..70596
                     /locus_tag="VQU32_000147"
     CDS             70414..70596
                     /locus_tag="VQU32_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET2471679.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLSFNEKFGTENT"
     gene            70606..70971
                     /locus_tag="VQU32_000148"
     CDS             70606..70971
                     /locus_tag="VQU32_000148"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET2471680.1"
                     /translation="MPNSENTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSAWEHLRAVTGI
                     DLLSILTDIANKKTGVKSK"
     gene            70968..72158
                     /locus_tag="VQU32_000149"
     CDS             70968..72158
                     /locus_tag="VQU32_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET2471681.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADM
                     FQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERK
                     RNGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQ
                     IIKIATDKNDIVLDPFCGSGTTLVASKILNRNYMGIDLSEEAINITQQRLENVIKTSS
                     NLLNKGIEAYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNEC
                     LNESISLLQNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKS
                     QQLL"
     gene            complement(72673..73665)
                     /locus_tag="VQU32_000150"
     CDS             complement(72673..73665)
                     /locus_tag="VQU32_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2471682.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
     gene            73825..75579
                     /locus_tag="VQU32_000151"
     CDS             73825..75579
                     /locus_tag="VQU32_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2471683.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISAVLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIVTPILVLVLGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAEVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAIIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            75563..77284
                     /locus_tag="VQU32_000152"
     CDS             75563..77284
                     /locus_tag="VQU32_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2471684.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERIHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            77295..77924
                     /locus_tag="VQU32_000153"
     CDS             77295..77924
                     /locus_tag="VQU32_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET2471685.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALIALGAFLGGILGAYIGKALLKKHFSNGLYCVGYFTPCLILWCYLN"
     gene            77825..78382
                     /locus_tag="VQU32_000154"
     CDS             77825..78382
                     /locus_tag="VQU32_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2471686.1"
                     /translation="MYRQGSIEKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIP
                     IVIRMFILPFMAASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKN
                     IKMAMRVRGINFKNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRV
                     KIQLIDFVYVLAVAGLIVGGLIWLK"
     gene            78370..79734
                     /locus_tag="VQU32_000155"
     CDS             78370..79734
                     /locus_tag="VQU32_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2471687.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGSGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDILEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDESSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLK
                     DISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVM
                     QDVNHQLFTDEVFNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAI
                     ASVMCKTSPFIFFDEPSSGIDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADE
                     IFEL"
BASE COUNT        24664 a        17093 c        14712 g        23754 t
ORIGIN      
        1 acctacggct agcttcctag tttgctcttt gattttcatt gagtattaca gtaaacaatt
       61 tttctagcta ttgtaacaaa aaagtcactc gaaggcaact tttttcgtcc ataaactttc
      121 aaaagagaat ggcttattca gaactaaaag ccgcagcttt ttgcatttgg taatacttac
      181 cctttctatc catgagttct tgatggttac catattcaac aatatcacca tctactaaga
      241 caagaattaa atccgcatcc tgaatggttg acaaacggtg agcaatgatg aagcttgtgc
      301 ggcccttcat gagttttgca aaggcatcct gtaccagcac ttctgtccgt gtatcaatgg
      361 aagaagttgc ctcgtctaag ataagaatct ttggaatagc cagaaagact cgggatatgg
      421 tcaagagctg agcttggccg acagagagag attctccagc attttccaac ttggtatcgt
      481 atccctgtgg caactgttgg atgaaaaagt ctgcattagc tgctttggca gcagcaatta
      541 cttgctctcg actggcttca ggattgccaa aggcaatatt atcatgaatg gtcccttgtg
      601 tgagccaggt ttcttgaagc accataccaa actgctgtct caatgatact cgtgtataat
      661 cataaatgga ttgcccatcc agcaagatat ctcccgagct aatgggataa aaacgcataa
      721 ggagattgat aagagttgat tttccagcac ctgtcggacc aacgatggct accttactac
      781 cagctggaat atcgatagac aactccttaa tcaaaatttt ttcaggatgg tagccaaaag
      841 agacatgttt aaaggaaata gctcccttaa cttggtcact ggtcaagact tccttacctg
      901 tttcagccac ttcagggcta tctaagaccc catagatacg ctctacgcaa gccagagcac
      961 tttgcaactc agctagcact gaagaaatat cgttaaaggg cttggtgtat tgctgaacat
     1021 agttcaaaaa agtcactaaa cgaccgacgg tcaaggttga acccatcatg atacgataag
     1081 ctcctactcc agctaaaagg gcataaatga gtgcatttac aaagcgagtc gaaggattga
     1141 ccgttgaaga ataaaagatg gctgactgag aatagcctga gtagttgtca tgagcctcac
     1201 gcaatctttg gataaattct gtttgagcat tgaaggactg gattatagtc tgctggctaa
     1261 gcgattcttc aatcaactga gtctgaattc ccctcgtctc tgtttgcttc tggaagagat
     1321 gataggatct cttggcaata aagcgtgaaa tcaccatgga cagtggcgtc aacagcaaga
     1381 ctaagagagt catgaggaga tgaatttgga gcatggctag aatactaacc aaaatcatca
     1441 aaacaccaat gaaaaattgg ttaaaaatca tggtcaagcc agctgccaac tgttcgatat
     1501 ccgtggttac acgactgacc atctctccat tgccttgccg atccgcaaaa gcaatcggta
     1561 accgatggag tttatggatg attcgctctc gtaaatctct ggtataagag aagattagac
     1621 gattatagag gagaggattg gcccattgta ccagagtatt tcctattacc accaagagca
     1681 tctggagaaa aatctgccaa aaaactggtg atgaaccagc cactaggact tggtcaatga
     1741 cctgcccaat cagaataggt aggtaaattg ataagccaac ttgggcaata gttcctagaa
     1801 aggctaggaa aaggaggaag ggatggtttg ctaaatctac gactaaacgt ttgagcgtct
     1861 ggtttacagt ttgtcgtttc attctagtcc tcctttccat gttgggatgc attgatttca
     1921 cgatagactt gactggattt catcaagtca tcgtgcttgc caacagctag caactcacct
     1981 ttttccaaga ggagaatctg gtccgccatc tggagtgtcg aagtccgttg agaaatcaag
     2041 attaagctca tatttggcag attttcgcgg atagctttca agagcttgga ctctgtaatg
     2101 gtgtccagtg ctgaggtcgc atcatctagg atgagaaacg gagcctggcg caagactgct
     2161 cgtgcgatag acagcctttg tttttgtcca cctgagaaat ttcgccctcc tgcttcaact
     2221 agagcatcca agagtccttc cttatcactg acaaaatcct tagcttgcgc aatctccaag
     2281 gcttgccaga gttcttgatc agtcacttct tgatttaaac ctaaagtcaa gttggaacga
     2341 atagttccct taaagagttc gaccttttga ggtacatagg caatccaaga ccgccactgc
     2401 tccaaattaa gaggactacg tccattttga taaaggtcaa tgttcccctt gtctactgga
     2461 taaagtccaa gtaagagttg caccaagctt gatttaccag aaccagtccc cccaatgata
     2521 ccaaggattt gtccttgagt catatcaaag gaaatgtctc tcagagaagg ctgggccgca
     2581 tcaggatagg taaaagtcaa ttcttggact tgtaaaaccc gaccactggt aacttgcttt
     2641 tgttctaatt ctgaatggat gttttcggga gattccgcaa agacctcctc gattctcttg
     2701 gctgagatat aggactggtt gagggaattg atcagcatgg ctagcttgac caattccacc
     2761 aaaatctgta agaggtaatt gataagagca atgagagcac cttgactgag tactcctcct
     2821 tgaattgaaa tatagccttg ccagataata acgagaagag ttccattgac aatcagatag
     2881 gtcagaggtg ttaataaact agacaagaaa cctgtctttt cttgtaatct agcataaact
     2941 tggttaaggg tttgaaaaat ctgtaactct cgtttttctt gaccaaaagc acgaataacc
     3001 cgcatccctt gcaattgctg gcgcgtttcc tgaaccagtt ggtccgtttt ctttctgaga
     3061 ctactgtaga gaggattgac caatcgagat aaccctacaa tgacaatggt caaaatggca
     3121 accatgacta agaaccagaa agtcaactca gctgagattc gataagccat aaaaatggca
     3181 ccaaaaacga taatgggcgc tcgtaaaaag agacgcagga attgattgat accagtctga
     3241 atctggtagg tatccgaagt caagcgagta accaaactag aagttgtcag acggtctctg
     3301 ctgtcctttg gcaaggaaag aatatgacga taaagatcgt ttgtcaattc cttagcaaaa
     3361 cctactgctg cctttgctga gtaaaattga gctatcaagg ccactaaaac gccaattact
     3421 gcaaagataa ggagcaggcc aatctgcatc cagagatgac cttgatctcc ctgaggtaaa
     3481 gattggtcaa caatcccagc aatcaccatg ggaaccaaga gctcaaaaac agcttctaac
     3541 agcttgaaca agggggctaa aattgattcc ttgatgtagg gtttgaagta agataatagg
     3601 tgtttcataa atcccttcta ttcatattct agaaatgaag aaagtgggaa gccccactct
     3661 ctgttttatt tgtttaagta aggcaataga tagccatatc ctgctttttc catctcatcc
     3721 ttggccacaa agcgtaaaga agcagaattg atacagtaac ggaggccgcc taactcccgc
     3781 ggtccatctg tgaaaacatg acccaagtga gcactgcctg aacgagaacg aacttcaatt
     3841 cgctccattc catggctcag atccttgtaa taatgaatca actctttgga aatcggacgg
     3901 ctaaaacttg gccaaccaca acctgaagca aacttatcct tggcaaaaaa gagtggctca
     3961 cctgtcgtaa tatctacata aatcccctct tcaaaggttt ggtcataggc attggtaaat
     4021 ggagcctctg tagcagcttc ttgtgtgaca cgataagact cttcagatag actggccttc
     4081 aacacctctt gactaggctt ttcatagttt gctgcatcaa tcaatggctt atcagcatcg
     4141 gtcacatcga tatgacagta acctgaagga ttcttcctga gatagtcttg gtggtagtct
     4201 tcagccagaa tgtagtggcg taattgctcc acttctactg caatctttcg acccagcatg
     4261 cgttcctgct cctgcaccac tgtgtagata gctggcaaat ctgcttcatc ctgataataa
     4321 atcccagttc gatattggcg accacggtca ttcccttgtt gattgataga tagaggatcg
     4381 ataactcgga aataataaag taaaatctct ctgagtgaca cttccttctc atcgtaaatc
     4441 acttggaccg tttctgcatg gtctgtttcc ttgatcaact ggtaattggt cgtttcgact
     4501 tgaccattag cgtagccaac actggtttct agcactccag aaatgcgtga aaaatattcc
     4561 tctaggcccc aaaaacaacc acctgctaga taaatttctg ccatctttat ttctccttta
     4621 tcacgttttt aactaatact cttcgaaaat ctcttcaaac cacgtcagcg tcaccttacc
     4681 gtaggtatgt tactgacttc gtcagttcta tctgcaacct caaaacagtg tcttgagctg
     4741 acttcgtcag ttctatctgc aacctcaaaa cagtgttttg agcagcctgc ggctagcttc
     4801 ctagtttgct ctttgatttt cattgagtat aaacttcttt tcacaaaaag gatatgaaac
     4861 cctctggtca agagtttccc catcctatct tctattcttt attttgcttc tacacggaca
     4921 cctttggtat caactctcaa gtcatgcagt tttcctttga aaggttgctt ttccaattct
     4981 gccttaattg ttggcatctt gtcatgagaa gccagaacca taactgtcgg cccagcacca
     5041 gaaaggtagg ttgcataggc cccattttct ttggtcactt gcttaatcat cgcaaattct
     5101 cttaccaagt cctgacgata gcgctcatgg aagaggtctc cctcgattgc ttgcccagcg
     5161 gtcaccatgt ctcctgccaa caaggcagca accgctacat tggcgataga acttgcagca
     5221 acagcttcct tataagacaa ttttttaggc aagacactac ggctgtcgcg agtacgtaat
     5281 tcatagtttg gaatgtaagc tagaaaatca cactctggaa agtctgctac gatagcagag
     5341 acttgccctt caacagaact tgcaataacg agattaccat aaatggctgg agccacattg
     5401 tcaggatgcc cttcaatctt ggtcgctaac tgcaattttt catggtctga taagttgagt
     5461 tgacccagtt ggttggctag ttcaatccca gcaacgataa ccgagctgga agaacccaaa
     5521 ccgcgcgcca aagggacatc actggtcatt ttcaagcgtc ttggttgcaa gtctggtaca
     5581 atttgcaaag cgattttaag caagagatta cgctcgtcat gtggaatcca tttgccaatc
     5641 tggtgttcaa tcagccactc atctcgttct tcgcagacct caatttgaag atacttggtt
     5701 acagctacac cgaccgagtc aaaacctggc ccgatattgg cactggttgc aggtacaata
     5761 atcttcatct tattctccta gcaccttgaa ggtattcaag aggtcgaatt ctgaaacctt
     5821 cttcaattca gctgagacat tttcaagctg ggctttatta atcttgtgtg tgatgataac
     5881 gacacgcgcc ttgtcaccct ctttgccatc ttgaaggatt tgcttaaagg aaatatcttg
     5941 agcattgaag atttcagcca acttcaagac ctgacctttt gagtctagag ccaagattga
     6001 gaaatagtag tttgctttga catcttcagg atttgccaag accaagtcac ggctatattc
     6061 gttgaagtct ttgccaatag taccatcatt caaacgacga acgatacgga caatatcagc
     6121 tacaacactt gttgcagttg gtttttgacc cgcacctggt ccgtagtaca tagactcacc
     6181 aataccgata gattctacaa agacagcgtt cattacgcca ttcacactag caagtgggtg
     6241 cgctttaggt aggaaggttg gagtcacttc tgcagcaata cctgaagaag tttcctcaat
     6301 agaaccaacc aatttcacta cgtaaccaag ctcttgagct acagctacgt cttctggtgt
     6361 gatattgcgg attcccttgt gggctacatc atcaaaggca atcttcatgc caaaggcaaa
     6421 ttggctcaaa ataaccatct tgtaggctgc atcaatccca tctacgtcat tcgtcggatc
     6481 gctttctgca aatcctagac gttgtgcttc cgcaagagca tcatcgtaag accagccttc
     6541 ttccaccatc ttggtcacca tgaagttgga agttccgttg actactccaa gcacgcgcgt
     6601 aattttatca gaagccaagg aatttgctaa agtacgaaga attggaatcc caccagcaac
     6661 tgctgcttcg tagtaaagtg ctaccttgtt agcttgagcg atttctagca attctgcgcc
     6721 atggacagct aaaaggtcct tgttagcagt aacaacgtgt tttccagctt ccaaggcacg
     6781 agtgataaag gttttagcag gctcaatacg ccccatcaat tccactacga tagtaatatc
     6841 ctggtctgat aaaatatcat ccacattggt tacaaagtta aagtcattcc ctgctgcaag
     6901 caagcgattt ttttcatctt catccttgac caatacctta gcaactttga tatctgaatg
     6961 tgctgattga ttgatttttc ctccattttc ctttaggagg aaaggcacac cacttgcaac
     7021 ggtaccaaat cctagtaaag caattttaac tgtcatcttt atctcctcga aattttctaa
     7081 tatagccatt ataacagaat tttgtgaaaa ttcctattat agtaaatcac tatttcagta
     7141 taaaaagaaa aaacgaatca gacgattcgc tcttcttaaa atctgaaaat agctttccag
     7201 aaaagattag ccgatttttt gcagattgag cactgcatcg tgactcatca agacttgacc
     7261 atactcttgt aagactgagc gactgatatc actatcgtct gcaaactcgc gcatacgggc
     7321 caacagccaa gctggatatg ggcttggatg attttcaata tccactaaaa tggtcaaata
     7381 atagcgctcg ttcattttgt agagttcaga agtttccatt tcaaaagtca ctgtcttggc
     7441 aaaagctacc aagtcagcca acttagcaaa agaaaggatg tagtagatgt aaggttcttt
     7501 cttactctca gcttcttgtt cagcctgctc ttgctcttct tccttggctt caacttgctc
     7561 aagagattga atggcttcga tatcatcctt ggttttgtct gcgatgcttt tttccagggt
     7621 tttgataaat tcatctggag acatttgagc caattcttcc atatctggca aatccgataa
     7681 gtcttcaaaa tctagatttt ggtcaatctt tgacttggtc acaaagacat ctaccttatc
     7741 aggttttgga gtcacacgga agctcaacat gcctgtatcc agaaagctat caggcatctc
     7801 tagctcatcc aagatagcat aaaagaactc ttctgttttt tcttgaggaa cgagaaagtc
     7861 agcaatctcc attccacgat ccatcaaatc ctctaaagac atcgtgattt ttaaagttgt
     7921 atcactaatt tgtttcattt tcattgctag taacctcata ctttcagttc tatctattat
     7981 actagatttt tacgatttta tcaaaagaag gctcctctat acggatagat tttccctagg
     8041 gtctttctat aggagactcc aagagaaaat ttctgcagac agatagaaaa agccttcaaa
     8101 atcggctaag agccgatttt gaagacctta tacatcagaa tacttataat ttaaaggttg
     8161 ctacaccgag gatagaacga tttaagtttc tgagaatttg aagactttgc tcaaatttct
     8221 tataacgagt cactccgtac tcttcaacaa gaaggactgt atctctttcc aaaagagatg
     8281 atacatcctg taaatctaca aaatgcattc cttttaaagc ttcttgactc tgtttcaatt
     8341 tatctaggat agctttattt gagctaacga tggtcaattc ctgtccagta tttttgtatg
     8401 acaaaacatc tgctaggtta gcaattgttg taatctctgt tacaaaatca atttgatact
     8461 gagaaaaatc acctactcta ttgattgttg gattaaagag ataaactaac acatttccca
     8521 tcacaaccaa aatcacacaa accactccaa taacaactaa acgaagaatc agatttttca
     8581 catttaagcc aagcgctgtt tcaccatttg cgttcaattc tttagagttg atggtttcca
     8641 gtttttcaat tttcacattt gcataggcat gtttaaattt ctcaatcaac ccatcaattt
     8701 ttttctctaa caagtcattg gcatctttac ttgatgtcaa aattttcaca ccaacccctg
     8761 catcgtcaat catatagtag acggtcaatt ttttccacca atagtcattc gttgaatttt
     8821 tcaaggttgt ttctgtcgtg tctaattcac tggcaatttt tttcaactca ctgggttcta
     8881 catcattgaa aagataagct ccattcaaat taccatcaat caatttccca taaaaatcac
     8941 tataaccacc aatttgatga ttcaaaatcg ttttggccga ctcttttgga ggagtgattt
     9001 tatagataag ataagttgaa taacttgttg tatctttgac agtgttttta ttcctaactg
     9061 ctttaattgt aaatggtaca gcaatgagag caaataaagc gatgagagct aaaatatttg
     9121 cttttcgctt tttataaaga tttgcaaaca aatcagctac tgaataatgt tcaaacatga
     9181 tttttttctc ctttgtttag tagatactag ttttcctttg taagcatttt tgctataaat
     9241 ataatcacaa gaacaattcc ccagaattgc attgtaaata aattgaagaa actttctgaa
     9301 aagctgcttc ttggcataaa gaatagatta ttcaagatga gtagggataa agcaaatagg
     9361 attgtccttg agcgataggc tacttgcagc atggctataa ataatacgcc gagtaagaaa
     9421 ctaagcagaa agactccaat cataccatag tcggtataca actccatgat ataactactt
     9481 ccgataccat gccctttcag gtattccttg ttcaagacaa gataggatag attgtgggca
     9541 taactattac tatcaatagc tagttccaca ctattggttg tatgttcaaa ggcttttcct
     9601 ccgaaaatgg ctcccaaact cccccttgca aaataatcaa gaacaggacc aaaagtaaaa
     9661 ttacggaaat ctcggtaagg gaggctactg ttaaatagaa aacctcgagc cagaacacca
     9721 aaactagtcc cttgtttata gataaagtca agtaagatat cccagaaacc tgtatgggaa
     9781 acttggacat tatcccgtac ataattgagt actcccatcg ctaacatgag aataggagaa
     9841 cctacaaaaa tcgctaactt ttctttaaac ccaatccatt ttcctttttc agtttgctcc
     9901 cgcataaagt aataaacaaa agcaaataaa atacttaaaa taaagggatt tcgtgtccca
     9961 attgccaaat gaatagtatt agctgcaata aaggagacaa gcactgctgt ggcctgcaat
    10021 ttctttggct tggttgccag atacatacac attgcataga ccgtaaaggt agacaaaatg
    10081 taggtaaaat aaggcagttt actttcaaaa tttgcatagt aggcatagta ggaagtctgc
    10141 aaacgataca agagccgttc aaataaccga atgaaataga aaggataagt tagaagaaaa
    10201 actcctagtg atacaaagcg taaccgcttg atataaacct cttttagaga atttcctata
    10261 tttgctactt ttattttctt cctagctatg aagtaacgag ccagaatgcc tcctgtggtc
    10321 aagcccagaa tcgaaaccat gacaactata aaggcaaaac gataggctat tggatgatag
    10381 gtatccaaag caccatccct aaaataatca atggtcggtc ttgataccag aaatacaaaa
    10441 atggttaaat agaaaataaa atggattaag taatacttga tatcattcca acaagcaatt
    10501 aagctactaa ccaacaagaa caataaagta gaaagtaagc taacattatt attattaaac
    10561 agatacacaa ttccacttac tagcgtcaag gcataactga ctatggtcaa actaaataat
    10621 aatcgtttcc catcaatcac ttggtcaccc ccgttctaat gtaatttttt agatttttca
    10681 atatttttca gtaataagaa tcgatataag gaaatattta tgaatagggc caaagcacta
    10741 attcttctcc ccttacggaa aattggattc ctagaaatag caaaggcatg gccttttaaa
    10801 aaacgatgaa tctgagaata ggcttcaaac tgtttatact gatcatctag caacatctta
    10861 tccagaataa agaagtgggc ataggccaat ctgaaaaaag cgacctcttt caagtcagga
    10921 tagtttttca caacttcatt ataaaacttt tggtagatat caatataggc taaatccttc
    10981 tctgcatagg gtttggtcgt aatactatcc cctctatgga aatagtaata atagggttta
    11041 gtattaacca catacttctt ggccaacttg attaaatcaa aatggtaata ggcatcttcg
    11101 taaatcaacc ccttaggaaa ggatagggca gttgcaatct gtctcttgat tagcttattg
    11161 caaatcgtcc caggtatttt ttcacctatg aggtattcct ttagaaatgt ttgagaatca
    11221 cagacaaaat agtcatcctg attggctgac tgtgggcttt catcattagc atagacattc
    11281 atgacaccac agcttgaaac atccgcatct tcttgaacta attgctcata taagctctga
    11341 atcatttctg gatggatata atcatctgag tcaataaaaa tcagataatc cccgtgagcc
    11401 tgcttcatcc catcatttcg tgcttgcgac aatccttcgt tctttttatg aagcactgac
    11461 accctgtcat cttgttcagc gattgagtca cacaagcgac cactttcatc tgttgcacca
    11521 tcatcaacaa gaataatttc cagattttga tagatctgct tctgaatgga agctatcgat
    11581 ttttctaggt actgcgccac attatagact ggcacaatca cactaattaa tgcagtttcc
    11641 atgctactcc tctaatagtt tttctacttg ttcgatttgt tttgtaattg taaattgttg
    11701 aatgaattgg ctagcctcat cgacatcaaa gtttgaggca gaagtcatgt aattagtaat
    11761 cgcctgagct gcctcttgat tgctctcaat gatttgtcca aatcgtcctt cttgggataa
    11821 ttcctcagcc cctccaacgt ccgtagagat aaaagggagt cccagactca aggcctccac
    11881 atacactcca ggaaaacctt cttgtttaga catagacaag agaactttcg tctgagataa
    11941 atactgataa ggattttttt gataaccaag gaaatgtaca tagtcctcaa tcccatactc
    12001 tttgactcgt tttttcagtt cctcttccat atcaccagcc ccgataaaat agagatgata
    12061 gttttttccc tcttggtgta ataatcgtat cacttccact acacggtcag aacccttatt
    12121 ttcctcaatc cgtccgatag tacagatact ttgaggagca atctcgatat cgatcttctc
    12181 ttgagatttt tctagaatag tctgaaaatc atatccattg tagattatct gtaatttaga
    12241 agcataatct ggataaactt ccttgataga attgctggtc ttttttgaaa tccctacaat
    12301 tgtattcgca gcatccaact ggcttctatg tgattctctt ttagagctat ccttaagaag
    12361 ttcttcaata cttccatgaa tccaagatat cttcttgact tctcttcttt tagagaacaa
    12421 cagtggtgga ttcataatgg taaaagaaac ttcaacatca taatcatctt ttacaagcaa
    12481 acgacgagtc agtcttggaa aataaattct cattctccac aaaaaagctc gtaaccatct
    12541 ggtttggcga taatcttgaa gggattttaa aatgcgtaca tgctttggaa cagattcata
    12601 tcccttgtca aagtgctcca tttcaagaat atcaatatca tacttttcta gatccagatt
    12661 tgaaacaatg gttgatagaa tcttctctgc accacctcca agagaaaaag accacataaa
    12721 aaataagatt tttttcttag ccaccatatt ctcccttgta ttctgtataa gacttatcca
    12781 tatcagcgat gacagcatca tgatgcggta cctgcttgtc tgctggtgga ggcgtcatat
    12841 aatccccaaa agcagttctg agatagacat catagccgat tggaataggc atctctgttc
    12901 cttcaaatgg caagaaaaga ttgtcttcaa aagatgtgat tgggtacttg tttctcatgt
    12961 agccaggacc tgagcataat tctgtaatgc catcacaatc agccaaatca tacttagtca
    13021 tttctttctc agcttttttc cagatgcgat aacggagaga ttttggagtc aaacccagta
    13081 aaatgcgact tccccatttc atgagagcac catgcttttc tggaatagtt tgcgcacaaa
    13141 agagtgaata aatcaaggcc caacgaacct gttttttccg ctcagctgga tttttcggat
    13201 aataatccaa aggcaaaaca tccaaggtca gaccatgtgg caaatctaaa tcctgctgat
    13261 aaggcttgat acaggtggtt tccttgtcac gaatggtaat aaaaagatta cgatcaacaa
    13321 aatccttgtg actctttgac aagaaataac gttcatctgc ataacgaggc cataattctg
    13381 ctaatttctc ataatcttta cgaggcataa aaaagtctag gtcgtcgtcc caaggaataa
    13441 atcccttgtt tcgaagggca ccaatagcgc ctccgccaca gagataacag agcaaatcat
    13501 gttctttaca aaaggccaca aaatattcag ccatctccag actacgagcc tgaattgctt
    13561 ttaaatcagt catattgttc attattcttt ctatcgtatc gtttcattat accacaaaca
    13621 aggggtgaaa atctattgca gactgtaaaa aatcaaagcc tgactgctat ccaaatagct
    13681 atcaaacttt gatttttctg tcttatactc ttcgaaaatc tcttcaaacc acgtcagctt
    13741 caccttgccg taggtatagg taactgactt cgtcagtctt atctacaacc tcaaaactgt
    13801 gtttttagca gcctgcggct agcttcctag tttgcacttt aattttcatt gagtattatc
    13861 ttatctcaag cccatttgag cgagcttggt ttgatatttg ttttgatcaa ccagcaagcc
    13921 caagcctcca taaacatcat aggcatctac ccagtcaccc agttctggaa tcgtcaattt
    13981 ttcaatacca ttttttgctc catccaaaac agataaaccg tttgttagga ggaaagtata
    14041 gggtacgttg gttgaggtca tagcaaaaac ctttccaaga gcttcagaac cagtgaaaag
    14101 tttagtggga tctttaattt gctctaaaat tgctgttaaa acttgttgct gtctttttgt
    14161 acggccgtaa tccgcctcat catcatcacg gaaacgagca taattgagca gggtcgagcc
    14221 attcatctgc tgttttccga ctttaatggt ttgggttgga gactcagtct cggtagcgta
    14281 taaatcatct ccgactgtag cttctgttag gggacgccca ttcaatgttg aaaattgagc
    14341 atcaatcgtc accccatcag ggaaaagcgt gtcaatcgct gtggcaaagg cctggaaatc
    14401 aaccaaggcg tagtacttaa tgtccaagtc aaaattatct ttcaagactt ggcgaaccat
    14461 ttctgcccct ttttgcccct cttgttctcc taactcgtag gctacgttta acttgttatc
    14521 tgtctgtttt ctaccattaa tcacttgact ataaccatct atatagacca aattatcacg
    14581 catgaaactg actagcttca ttttcttatc tgagcccccg acatttaata ccataataga
    14641 gtcagttcgt gtctcaacac tgttctggcc gattcgacca tcagtaccca tgattaaaat
    14701 attaactcca tctctagtgt cctgaccatt aaagacttct acttgagctg cccgggcatc
    14761 agcagttttc tttgcgctag catcttggta accacgcaaa aacatgaata ccatggccaa
    14821 agccacacag accaaaagtg aaaaaatcac cataaaaatt cgtttaagac ggagcttccg
    14881 tcttttcttt tttggaggga aagagagtgc ttgtgatttg gattgtgagc gactccggtt
    14941 cgcatagctt ggtaagtcaa cctgctcttc tctttcttgt tccaagctag agctactatt
    15001 tcccctagca agagttagct tttcttgcaa ataggcaaac tcattttttt ctctctcatt
    15061 gagatagtga atatttttta gcaaataatc ataacgcaac tgctcatgat gacttaaggg
    15121 attttcttta ctcatcttct ctcctttcca tggtctgata ttggataaat aggataggca
    15181 cccagaattt tatactggat tccaatcgct tctaattctt tttgggcaaa gtggaccaag
    15241 tccttatcga tataatccac atcgataatg aaaaagtatt cacccagtgc tgtcttgagt
    15301 ggacgacttt caatttttgt caagtcaatt cctcgccaag caaaggtcga cagggcctta
    15361 taaagtgcac ctggaaggtt gtcaggtaat gtcaaggcca aactcatctt ttcagtttgt
    15421 gcttgcaagg gaatactagg cttttcagct cctagaaccc agaaacgtgt gaaattggct
    15481 tccatttcct gaatatcctc ggcaatcagt tccaatccat attcttcagc agaacttcta
    15541 ggtgcaactg ctgcaaaggg ctggtctgga tgttcggaaa taaaacgggc cgcataagct
    15601 gtactagctg ttacctcgat ttgagcctct ggatattgtt catcgatgaa tttctttcct
    15661 tgagccaagg cctgtggatg tgaaaaaatc ttttcaatct tagtatggcc tggaaccacc
    15721 atcaactgct gatgaatagg ctgaacgatt tctgctactg cttggatgtg agcctgatga
    15781 aaaagatagt ccaaggtttc atgaacacta ccctcaatag aattttcaac tggcaccaca
    15841 gaatagtcca ctaatccttg ctcataagcc ttgatgacat ctgtaatgtt ggcaaaagcc
    15901 tgcaattcct catgaggaaa agctgtctgc acaacgtggt gtgaaaatga tcccttggga
    15961 cctagataag ctattttcat cttagttcct ctataatttc ctctgggctt agcttggtca
    16021 catccaaaac ccgactagcc acttcctcat accaagcctg tctttcttga aaaatagcta
    16081 ctagttcttc cttgctatta tttagaaaaa gcggtcgctg attgtcctta tcagctgcga
    16141 tacgtaggta gagggtttca aaatctgctt tcaggtagat gttatctgta ttagtcttga
    16201 gtaagtcacg atttctctga gaaataacca ctcctcctcc agttgacacg acttggtctg
    16261 tttgtagtaa atcagctagg acttctgatt ctacatgacg aaaggctgct tctccctttt
    16321 cagcgaaaaa attcgcaatg gacataccta ggcgcttctc aatcagagca tccatatcaa
    16381 ggtaattagg gtccaagcct cttgcaatag tcgattttcc agcccccata aaccctaata
    16441 acaccttagc catgaatcaa gctctccaaa tcatcaaaga aactaggata gctggtattg
    16501 atggcttctg cacggtcaag ctccacctct ccatctgcaa ccaagagggc tgcgatagct
    16561 gtcatcatgc cgatacggtg gtcaccaaac gtattgactc tagcaccgtg aagagctgat
    16621 ttccctttga taatcatccc atctgccgta ggagtaatat ctgctcccat actatttaag
    16681 gcgtctgcca caacctgaat acggtctgtt tccttgacct tgagctcctc agcatccttg
    16741 ataactgtta caccttgggc ttgagtcgca agcagggcaa taatgggcaa ttcatcaatc
    16801 aatcgtggaa tcaaagcgcc accaatctct gttcctttca agtcagaaga ctcaacaatc
    16861 aaggtagcag atttagcgac tggatcgatt tcagttattt ccaattttcc acccatggca
    16921 cgaatgacat caataatacc ggtgcgagtt tcgttgatcc ccacattctg cagcactaga
    16981 cgagaatttg gagcaatcaa acctgcgact aaccaaaagg ctgcactgga aatatctcct
    17041 ggtacgacca ccttctgtcc tgtcaatttt tgtggcccct ggactgtgat tttcttacca
    17101 tccacactta aatgaccacc aaattgttgc aacatatctt cagtatgatt acgggtgtac
    17161 tctttttcga taataactga ctccccctta gcttgtaagg ctgcaaacat caaggctgac
    17221 ttgacttggg cagaggcaat tggcaactca taatgaatag gtcttaggtt tttcgtccct
    17281 tttaagcgaa ggggaggcaa gtctcgttca gtttgccctg aaatgctgac gcccattttt
    17341 ttcagtggaa gggtcacacg gtccatagga cgtttggaaa gactatcatc tccaaacatc
    17401 tctacttcga aatctgcacc agcaaggaca cctgaaatca ggcgaatcga ggtgccagaa
    17461 tttcccatat taagggcatt ttgtggcgct tttaagccag ccatgcctac accttgaatg
    17521 gtaataaccc catctttatc ctcaatttca acaccaaggt cacgaaaaac ctgcatggtc
    17581 gaaagaacgt cttcacctcg cagaatatca taaaccttgg tctcaccctc agccaaactt
    17641 ccaaagataa tggaacggtg gctgatagac ttgtcacctg ggacgcggat actaccatgt
    17701 aaatggcgaa tgtttgtttt tagtttcata ctggacctca tacttgcaat acttttacct
    17761 attttatcat aaaaagtcag aaattcctta aaaattcctg actttaggat cgttcttttc
    17821 ttatttcagc aattctgaaa ctggttcaaa aacaattttt tcaatgtcag aaaggtaaat
    17881 ggccaattgt tgttgcttgg taaagaattc tgacaagagg ctatttcctt gaatctgttt
    17941 accaaagcct tccatcttag cttggaagga cgcatctggc atttgacctg tctgtgctag
    18001 tttttgaatt tcctcttgaa aggcaagata ttctgtaaag attttgcttg cctcagcatc
    18061 tgctgcaatc gcatctttag ctgctttaac agccttgtat tctggtaatc cgcgtagacc
    18121 gcgactgagt tcgtttgcac tatcgtaaat atttgacatg ttcttctcct tatttgatga
    18181 cgactgtata gtcggtattt tctgttatga gatgctcagc tctttccaag tcttgagcat
    18241 ttttaaatga aatttgtagg attccgtgaa tatcctcacg attttcctcg ttgatgtgga
    18301 tgttaaccaa ggaagttcca cgtagcagtt ccaaaatccg caggatgaca tcttcttcat
    18361 caggaacgtc aacatagagg tcgtaagagc tatccacacc accacgctta tggatttcca
    18421 tggtctggcg ttgttcacgc gcttggttaa aaaagttcca aatttgctct tcatctccct
    18481 tactaatggc ctgaccaatc acttccaaac gttccttgaa atcctcaatt ctatccagaa
    18541 tggtctcgct attggacaag agaatggagg tccacattcc tggctcgctt tccgcaattc
    18601 gggtcatatc tcgaaaacca cctgccgcaa agcgccttgc catctcatgc tcttgagcat
    18661 agaccgcagt ctgctccatg agactagaag ccaaaatatg aggaaaatgg ctaatctgag
    18721 aagtgacacg atcatgctcc ttggcatcaa tctcgataaa acgagcatga agacctgaaa
    18781 gcagatcctt catttcctta agcgtgtcct gacttgtcag gcttgaaggt gtaaagatat
    18841 aataggcatt ttcaaaaaga ttgacatctg ccgaagcagc ccctgtcttg tgactaccag
    18901 ccatgggatg ggccccgaca aagcgaacag acttgccagc caaatactgc tccgccgcat
    18961 ccacaatggt tgacttggtc gaaccagcat ctgaaataat aacgccttct cgcaaatcca
    19021 aattggccaa ctccttaatg aaagcaatag tttgtttgat tggcaagctg aggataatga
    19081 catctgccaa aggagcaaaa ctagcaaaat catccgttgc acggtcaatc ataccttctt
    19141 tcaaggcgat atctctcgaa gcttgactac gattataacc taaaatttca taatctggat
    19201 gatcgcgttt gataccaagt gccatagagg ctccaatcaa cccaagacct gcgatataga
    19261 ttgtttttgc cataggaact ccttaatagt tctttgtata gtctcggtgt ttggctaccg
    19321 cttcttttag ttcctcaaga ttatctgatg agaatttttc gaggatttct tgcgccagaa
    19381 ccgttgctac aactgcttcc atgaccattc ctgcagctgg aagagcagtc ggatcacttc
    19441 tctccacggt tgccttgtaa ggttcgtggg tttcgatatc cacactcata agaggtttat
    19501 aaagagtagg aatgggtttc atgaccccac gaacaacgat gggttgccca ttagtcatac
    19561 caccttcaaa accacctaga ttattggtac ggcgagtata accgtcttct ttagaccaga
    19621 gaatttcatc cataacttgg ctgcctttac gataaccagc ctcaaagcca agaccaaatt
    19681 ccaccccttt aaaggcattg atagagacaa cagcttgagc caatcttgca tccaattttc
    19741 tatcccattg gacataggaa ccaagaccaa ctggaacgcc tccgacgact gtctccacaa
    19801 ccccaccgat ggtatcacca tcacgtttga tttggtcaat atagtccttg atttcctgtt
    19861 ctcgttcttg gttgacaata gaaacttcag actgggcagc tctttgctta atttcagcga
    19921 ctgtcagatt ttcaggaaca tctatttcct taccaccaaa gaccacgaca tggttggcaa
    19981 tctccatatc cagctcagcc aagaggcgtt tggctactgc accaactgcc acccgcatgg
    20041 tggtttcacg agctgatgaa cgctccaaag aatttcgcaa atcatcaaaa cggtacttaa
    20101 tccccccaac caaatcggca tgacctgggc gaggatgagt aattttccgc ttgcttttaa
    20161 ggcggtcttc aatgtcctcc gcagacatga tgtccagcca tttctggtgg tccttattga
    20221 tgacatccat agtaataggc gcccctgtcg tcttcccgtg gcgaacaccc gaagtaaaga
    20281 caacctggtc actctcaatc ttcatacgac caccacgacc gtagccaccc tgacggcgtc
    20341 taagatcctc attgatatcc tcagctgtca atggaagtcc agctggaatt ccctcaataa
    20401 tagctgttag acgggggccg tgtgattctc ctgcagttaa atatctcata cactctcctt
    20461 attttaccaa gtagtctttc atctcttcca gagaaactgg gtgaatggtc gctgaaccaa
    20521 gctctggcac caagaccaat ttcaaggtgt taccacgcgc tttcttgtca tgagtaagag
    20581 cctgataaag cttgtcaact ttccaatttt catagtcaac aggcaagccg aatttctgac
    20641 acatctctgt gatagattgg gtaatgccag ctggcatgag gcctttttcc tcagcaacct
    20701 tggaaatctg taccattccc atggcaacag cctctccatg catgaccttg ccataaccgg
    20761 cagtcgcttc gatggcatgg ccaatagtgt ggccaaaatt gaggtaaaga cgaataccat
    20821 tgtccaactc atcttcaacc accatcttgc gcttcacctg acaagaatgt tcaatcaagg
    20881 tctctgcatg ttccaaaata ctctcaacag aaccattcag tcccgtcaag agagcccaca
    20941 gttctggatc ctcaatcaag ccatacttga taacttcacc catcccttca atcaactctc
    21001 tttttccgag ggtttcaaga acaagtggat caatcagaac cccatctggt tgggcaaagg
    21061 tccccaccat atttttagca aatggtgtgt taacgcctgt ctttccaccg atagaagaat
    21121 caacctgagc tgtcaaacta gtcggaatct gaacaaagtg aataccccgc atataggtag
    21181 aggctacaaa gccagccagg tccccaacaa cgccaccacc aagagcaacg attccatcgc
    21241 tacgagtcag accttgcttg actagaaatt catagacttt ctgaacagta gttaaattct
    21301 ttctttcttc accttctaag aaatcaaaaa cagctacctg aaaaccagca tcttctaggc
    21361 tgagcttgac cttctctgca tagagagagg ctacatggtt atctgtcaca atgactacct
    21421 tttgcggttg ccagagttct cgcaaccact gaccagcctg ggccatacaa cctttttcaa
    21481 tctgaatatc ataaggatga tgaggaatat cgattctgat tttcatagga gagtctccct
    21541 ttctttattg atatttttct gttaaagact gccaaatctc ttctgtcggc attttcttgc
    21601 ctgtccacag ttgaaaagct tctgcagctt gatagagcaa cattcccaga ccattgactg
    21661 ctggattgcc ctgacttcta gcccatttca aaaacggtgt ttcaaagggt tggtatatga
    21721 tatctgcaac taagagagtt tctggtaaga ctatgttttc aggaacagga gaggattggc
    21781 catccatgcc cacactggtg gcattaacta gcaaatccga ctcggcaatc cttgcttgca
    21841 gttcagaaac atattctaaa gcacacaaat ccactttaaa gcctgtctgc tcctgtaact
    21901 tgtctaggta aggtcttgtt ttttccatag aaacggaacg aacaaagacc gaaatctgac
    21961 tgacgccatc caaaatagcc tgtgccaaga ttgatttagc cgcaccacct gcacccagca
    22021 gggtcatctt tttacctgaa attgtaaaag aaggcaagca cttaaaaaat cccttgccat
    22081 ctgtattata tccaattaaa ttgccattct cattgacaac cgtattaacc gcaccaatca
    22141 agcgcgcttc atcgctcagc tcatccaaat aaggaatcac ctgctcctta tagggcatgg
    22201 acagattgat gccaaacatc tggtagcgac gaatattggc cactgtttct accaagtcac
    22261 tcgcttcaat ctcccaagcc acataagcac cgttggtagc tgtcgcctca aaggctctat
    22321 tgtggatgaa gggagaaata gaatgcttaa taggattggc aacaactgca gctaaacgtg
    22381 tatagccatc aagcttcatc caaaatctcc ctgatttttt tcatgctagc tagagaaatc
    22441 tgcccagggg cactaacctc atccagactg gcaaaagacc aactcgaacc agtcacatcc
    22501 gcagtgatac gagagacctt gcccacctta cccatagaaa tggtcacata ttcctgttca
    22561 ggattgagcg ttttaaagcc tcgtgtatag ttcatcaagt ctaagacatc ctgctccgtg
    22621 tgagccatca ccgcaacctt aacaagtttt ggatttagga tcgtcaactc tgacaagatt
    22681 tccatcatgt tctcaggtgt ttcttggaaa ttatggtaac tcaaaacaag atttgggaag
    22741 tccagcattt cctcaaaaac atccttgtag ctatagtact caaaatcaat atagtctggt
    22801 tgatagagtt gcgcaacttc cttgattaga tggatatact cttctggaga aaggtcgatt
    22861 tctccacctt cggagcgagt tcgtagcgtg aaaaccaact cacggcctgc gaatttttca
    22921 aaaatggctg gagctacctg caaaatcgct tctttaggca gatagtcggc acgccattca
    22981 atgatgtcgg catccaggta cctcgtggca tccagagcct gagcctcctc taaacttctt
    23041 ggcattactg aaacgattaa tttcatttac taaccttcat actaatcacc ttgaggtaat
    23101 tactactttc atctttttta ttataggcaa aatctgctgg aagaccatat ttgtttaaaa
    23161 tctggtaact tcttcctgca aagcctttat caatttgttc tgtaaatttc tgacgggaaa
    23221 cattggcagc attggtactg gcaatgataa tccctcccgg atttaaaatc tcaagactct
    23281 gggaaatcaa cttgtgataa tccttggcca cagagaaagt ttgtttttta ttccgagcaa
    23341 agctaggcgg atctaggaca atcacatcgt aggtcaagtt tttgcgtttg gcatatttga
    23401 aatactcaaa gacatccatg actataaaac gacgctcgtc tgtgctgagc ccatttgcct
    23461 gaaaatgcgc ttgagacaat tctcgtgaac gtttggctag atcaacagaa gttgtctggc
    23521 tagctcctcc catggccgca gctactgaaa aagccgctgt gtaggaaaac atattgagca
    23581 aggatttacc catagccaag ccgtcaacta aactaccgcg aacctcatgc tggtctagga
    23641 aaattcctgt catcaagcca tcattcataa agacttgata caggacacca ttttctaaaa
    23701 cagtgaaaaa gtcaggtgct tcttgaccat aaacatgggc agattcatag tccaaaccct
    23761 taaagcggat tttctcataa gcccctaaaa cctcagggaa aacctgtcta aaggcttctg
    23821 atatagtctg acgaatctga taaacataag agttatacca agaaaagacg gcgtagtcgc
    23881 cataaaggtc cactgtcaga cccccaaagc catctccctc ttgattaaag agacgaaagg
    23941 cagttgtcaa atcatcttga tagtaggcgt ttctcttttc tttggctttt ctaaacaacg
    24001 tttcaaagaa agcttgattg aaggccacct tgtctttgct gacaaaccag cccaagccct
    24061 tgttttgctg agaaaggtag gcagtcccaa gaaagtttcc ttcctgaccc tgcacctcta
    24121 cttcctgatc cttaagattg acattctcaa gatcactggc ttctagtaaa actagcccct
    24181 tagcaagctt cttttcaacc cttttgctga ctcttattct attcataact accattatat
    24241 caaactttta gacaattctc aaaaaagaaa ctacccttgc ttttttactc ttcttttaaa
    24301 aaatggtata ctagacttcc tgcaaaacta ggaagtaaat gtgtaagaat cacaataaaa
    24361 aatgctcttc cgtcttggag gagcatttct ttttatcaac gaaaatcaaa taacaaacta
    24421 tgaaactagc ctcaggttaa ctgtgagatt ataggtagag aggttgaatc agcaatatgt
    24481 gtctgtcaaa tttagtgaca aaggtagtag aagaaagatg aagaaataaa tctgcttcag
    24541 tagatatctg gaaaatttga ttttatagaa aagccttttg ttacaaactc aatatactat
    24601 caataaataa tattatagaa gcaacaataa ttaaaatttc acctatttgc atcattctat
    24661 ttcgaactct aaatatatgt tctatcaaaa atacttggat cacacacatt ataggaatta
    24721 acgtttttga aattgaaaaa tatcccaata aataaactat aaacaacaat aatagaacta
    24781 tattatattt cttattcaaa acattcctcc ctatattttt gattaccaat cttaatcatt
    24841 tacaactaca ttctaacaaa ctataaaagc gtttgtcgaa ttgaatttat caagcaagca
    24901 accaactatc tcatcttttt tctatttctg ccaatatgcg tgacaggtaa taatgatagc
    24961 caaaaatagc aagagcaagc aagacgataa gagctcctac tcccaagttg atggcaagga
    25021 taggggagag agactgaacc aagaatatgc tcccaattac aagggccatc aggattgcac
    25081 tataaataaa caataaaact atggcgacta tgccatttga acgattcacc aggtccgtaa
    25141 tgctactcca attggttgac agatttttaa cgtccttaaa gtaatggtgg caagaaagga
    25201 tgacactggc aatgatccag actacaagaa ggtaaatcat cgaaaggatg gacaagccta
    25261 gatatagaga aagaccgagc aaagtcagaa ctggtaaaaa ggactgaaca gcaaagataa
    25321 tccaaaattt cactttcaca taacgagcaa agtcaaaggg taaactcttc agaaaatcaa
    25381 cattttccct ctccaaggac aaggcaattg aatgcaggct ggtgatattg ttattgacaa
    25441 ctgctataaa cactcctaca aaaaacaagg gtaaccagta tggaggatga atgtctggaa
    25501 ctatctgaga atctcggatt ttggaaatca gactgatcat catgagataa ggaaggaaag
    25561 cacttgtaaa aagcactgta atcacaccag tcccctatcc caagagggtg aggtggtagc
    25621 gtaaaaccat acggaaaaag ccctttttag tagttgaaat cctctccttg ctgcgacgtt
    25681 cttttttgac cttctcctca ctgttaagca ggatcacgtc ataaaaacga ggaaggacct
    25741 tctttttggt cagataaagc aggaagagag ttagtcctat ccaagcgagc agacccacta
    25801 aggcttctgt cgaaaaaggc tccactgcta ttttgtaaaa gatatgaaga ggataaagga
    25861 gaaatggaat gtctctaact ttgtcaacaa tacttccaaa agtcgactga agaaagaaga
    25921 taaatattaa aggtatgaga actcctatcc caatcatcac attcgaaaaa atagactgat
    25981 actttctgaa gaccctagtc tgagccaaga aatgtactgc cactaccgtc actaaagtaa
    26041 cagagacaaa taataaggtc aaggacagta gcatcaaagg caaacccagc caaagagaag
    26101 gagctagact aatatagagg gctagaaaat aagctaggat tggtacaatt ccagttagag
    26161 ctggcaagag gacagacaga cctttggcca ttataatctc tgattcttta aaggcatagg
    26221 gcctatagga ggccaggtcc ttactctcat aaaagacatt gtaaaaggca gtgaaagaag
    26281 ttgaaaaggc cagcaccagt aaaatagcaa ccatcgagct aaaataaata ggtatttcct
    26341 caaaaggaaa atgaatggct atattactaa aacagatgag catcaagaga ctggaaaaaa
    26401 tgtaagaact taggactcta gcagaaacat ttactttttt cccaggattc ttagcctgct
    26461 tctttcgtag attagccaga ttagcttctt gagatgaata gaggatattg atatcaacta
    26521 attttttaat gaccttgaga cgcatccgca acctcctctt ttctaccagc aagactaagg
    26581 tagatacttt ccaaaaactg gtctgggtgg tctttcctca agttctctac actaccacaa
    26641 tagatcaaat gccccttttt caaaatagca atccgatcac agacttgctc ggctacctca
    26701 aggacatgag ttgaaaacaa gaccgtcttg cctttttgtg catgttcctt catcatttgc
    26761 ttcaaatcaa aggcggcctg gggatccaaa ccagtcaagg gttcgtccaa aacccaaata
    26821 tcaggagcag acaagagtgc cccaatgaca aagactttct gacgcattcc gtgagaaaga
    26881 gtttcaataa cctgatagcg gttttcagca aaatcaaaaa cgttcaatag cctagctaga
    26941 ctagtctcca agtcagagct agtcagatca taggatgaag cgatcaattc ccaaaattca
    27001 ttggccgtta agcgtaaaaa taagtcaggc gagtctgcta cgtagccaat ctttcgtttg
    27061 atcgccaagc gattttccga taactcctga ccgtctacca aaatacgacc actgctgggt
    27121 gaaatgatac tgactaggga ttttatggtg gtcgattttc cagccccatt atgaccaatc
    27181 aagcccataa tctctccatt ttcaatctgc aaattgagat tactcaaggc ctctttatca
    27241 ccatacaact tactaacatt ttgaaattca atcatgggac aactcctatc tttatctgta
    27301 ttatacatcg ctatactagc actttgatac aaaaaaatca atactttatt ttaagtagtt
    27361 aaaatagttt ccttctatct tacgcaaacg tttgcgccga gagatacttt atgatagaat
    27421 aaagaacaag attgacaagt aagaggaaac atcatgcaaa atcaaacact catgcaatac
    27481 tttgaatggt atctgcccca cgacggtcaa cactggacgc gtctggctga aaatgctcca
    27541 cacctagctc atctggggat cagtcacgtc tggatgccac cagccttcaa ggcaaccaac
    27601 gaaaaagatg tcggctatgg ggtctatgac ttatttgact taggagagtt caaccaaaaa
    27661 gggactgtcc gcaccaagta tggtttcaaa gaagactatc ttcaagccat tcaagccctt
    27721 aaagaacagg gaattcaacc tatggccgat gtagttctca accacaaggc tgctgccgat
    27781 aacagggaag cctttcaggt tatcgaagtt gatcctgtag accgtacagt tgaacttgga
    27841 gaacccttca ccatcaatgg ctggactagt tttaccttcg atggtcgcca agatacctat
    27901 aatggcttcc actggcattg gtaccacttc accggtacag actacgatgc caaacgcagt
    27961 aaatctggga tttatctgat ccaaggggac aacaagggct gggctaacga ggaattggtc
    28021 gataacgaaa acggaaacta cgactacctc atgtatgccg acctagactt taaacatcct
    28081 gaagtcatcc aaaacatcta tgactgggct gattggttca tggaaacgac tggtgtagct
    28141 ggtttccgtt tggatgccgt taagcatatt gactctttct ttatgcgcaa cttcatccgc
    28201 gatatgaagg aaaaatacgg tgacgatttc tatgtttttg gtgaattttg gaacccagac
    28261 aaggaagcca atctggacta tctcgaaaaa acggaagaac actttgacct tgtcgatgtt
    28321 cgtctccacc agaatctctt tgaagccagt caagctggcg caaactatga ccttcgtggc
    28381 attttcacag atagcctggt tgaactcaag cctgacaagg ctgtgacttt tgtcgacaac
    28441 cacgataccc aacgaggaca agcccttgag tctaccgttg aagaatggtt caagccagca
    28501 gcctatgccc tcattttgtt acgccaagac ggccttccat gtgtctttta cggagactac
    28561 tatgggattt caggccagta tgctcaagaa gatttcaaag aaatccttga ccgcctccta
    28621 gccatccgaa aagacttggc ctatggagaa caaaatgact actttgacca tgctaactgt
    28681 atcggttggg tacgttcagg tgctgaaaat caatccccaa tcgcagtcct tatctcaaat
    28741 gaccaagaaa acagcaagtc aatgtttgtc ggtcaagaat ggactaatca aacctttgta
    28801 gatttacttg gtaaccacca aggtcaagtt acaattgatg aggaaggtta tggacaattc
    28861 cctgtctcag ctagatccgt aagtgtctgg gcagtcaata ccatctaata gctcataata
    28921 accaagctag gtccaagcgg atttggcttt tttgtattca caaaaagacc tacccaaatg
    28981 gatagatctt tacttgatta caatttacct gctactgcat ccaacaattc ttggatctta
    29041 gcttggttgc ttcctcctgc catggccatg tctggtttac caccaccacg tccatcgatg
    29101 attggtgcta attctttgac aaggtttcct gcatgaaggt cttttgtctt gcttgctaca
    29161 aggacattga ctttgtcacc gatagcggca actaggacaa gaagatcaga gtagtctttt
    29221 tgtttccagt tatctgcaaa agtacgaagg gcaccggcat cggatacaga cacttgacta
    29281 gcaatgtaac gatgaccgtt gacttcctta gcatctttga agatatcgcc tgcggctgca
    29341 gctgcggctt tttctttcaa ctcagcattt tctttttgaa gttgacgaag ttgttcttga
    29401 agtccttcta ccttgtgagg tacttccttg acttgaggtg ctttcaaggt tgctgcgaca
    29461 gctttaagag catcctcttg ttcacgatag gcttcaaagg cttccttacc agtcactgcc
    29521 aagatacggc gagttcctga accgattcct tcttctttga caattttgaa gagaccaatc
    29581 tcagaagtgt tgccaacatg agtaccacca caaagttcaa tagagtagtc accgatagtc
    29641 acgacacgaa cttccttgcc gtatttctca ccaaagaggg ccatagctcc catttcttta
    29701 gcagtgtcaa tatccgtttc aactgtcttc acttcaagtg cttcccaaat tttctcgtta
    29761 acttgctgtt caatcgcacg aagttcctca gcagttactg cttggaagtg ggtaaagtca
    29821 aagcgaagga attcaacttc gttaagagat cctgcctgtg ttgcgtggtt tccaaggata
    29881 ttgtgaaggg cagcgtgaag caaatgagtc gcagtgtggt ttttcatgac acggtgacgg
    29941 cgattgctat caattgccaa ggtatattct tggttcaagg caagcggtgc aaggacttca
    30001 actgtatgaa gggcttgacc atttggggct ttctgaacat tggtcacagt agccacaatc
    30061 ttacctgact catccaagat ttgtccgtag tcagctacct gtccacccat ttcagcataa
    30121 aatgacgttt ccgcaaagat aagagaggca gttccttctg aaacagctcc tacttctgca
    30181 ttgtcagcaa cgatagctac caatttagaa gacaattggc tagtattgta gttgaagaca
    30241 ctttctacag tgatgttttg aagagtttca ttttgcatac ccattgagcc acccttgaca
    30301 gctgacgcac gcgcgcgttc ttgttgttct ttcatggctg cttcaaaacc ttcacggtct
    30361 acagtcatac cagcctcttc agcaatttct tcagtcaatt caactgggaa cccataagta
    30421 tcatagagtt tgaagacatc tgaaccagcg ataacagatt gacctttttc tttcaagtct
    30481 gctacaatgc cttgggcaaa gtgttgacct gagtgaaggg tacgggcaaa tgattcttct
    30541 tcgctcttaa cgattttctc aataaagtca cgtttctcaa gcacttctgg gtagtagctt
    30601 tccatgattt ttccaacagt tggaaccaat ttgtaaagga aaggctcgtt gattcccaat
    30661 ttttgaccat gcatagaagc acgacggaga agacgacgaa gaacataacc acgaccttca
    30721 tttcctggaa gggcaccatc accgatggca aatgaaagtg aacggatgtg gtcagcgatg
    30781 accttgaagc tcatgttgtc gccatcttgg tcataaacct taccagacaa tttctcgact
    30841 tcacggataa tcggcatgaa gaggtccgtt tcaaagttgg tcttagcccc ttggataacg
    30901 gccaccaaac gctccaaacc agcgcccgta tcaatgttct tatgtggcaa ttccttgtat
    30961 tcgctacgag gaacagcagg gtctgcgtta aattgtgaca aaacgatgtt ccagatttca
    31021 atataacggt cgttttcaat atcttctgca agcaggcgaa gaccgatatt ttctgggtca
    31081 aaggcttccc cacggtcaaa gaagatttct gtatctggtc cagaaggtcc cgcaccgatt
    31141 tcccagaagt tgtcctcaat tggaatcaag tgacttggat ccactcccac ttcaatccag
    31201 cggttgtaag aatctttatc gtctggatag taggtcatgt aaagtttttc agcagggaaa
    31261 tcaaaccatt cagggcttat caaaagctca taagcccaag tgatagcttc gtcacggaag
    31321 taatccccga tagagaagtt ccccaacatt tcaaacatgg tatggtgacg cgcagtcttc
    31381 cctacgtttt cgatgtcgtt ggtacggata gccttttggg cattggtaat acgtggattt
    31441 tcagggataa tggtcccgtc aaagtatttc ttaagggttg ctaccccaga gttgatccac
    31501 aaaagagttg ggtcatttac aggaaccaaa cttactgatg gttctactga gtgacctttg
    31561 gtcgcccaga aatcaagcca catttggcgt acttgtgcac tagatagttg tttcatattg
    31621 tctccttatt tacttgttta atgtgattgg ctttccagca tttccacata gtcaatcgcg
    31681 acacagaggg aaatgactag gtctgcataa gcgtcttcaa gaaccgttac ggtataggta
    31741 gaggtcagat ggaagagttc cttcttaatt tccgcaatca actgatcgcg atcatccagc
    31801 aatttgaaat tcaaatccca gatattgccc tcgatacgaa gacctagatt atcaaactca
    31861 tacttatctc gccaaaaggt caacttctta cgaatgacaa aactcgagcc atccagaagc
    31921 tgaatctcaa aacgaggaag caaggtcaag atttctttac tgatctgact gacttgttca
    31981 ccagccgcat catagatggt aaaagttttg ggaatcttaa aaaatgatcc ctccacctga
    32041 taggcaattt ctcccctgtc atccttgata gcgaagcgtt cgcctccaag acgaaacttt
    32101 tgtttgacaa gaaatgtttt catcaacacc tccaaaaatc aaaagacaag ctcatatcac
    32161 gaagggcgaa aaaccgcggt accaccttca ttcaatgaac ttgtcattct cttgttctta
    32221 tgcaattgta tgattgagta gcatgacttc ctagcttaga tggctcgcag caccgccatt
    32281 tctctggact aagacaagtg aaaatcaatt ctcaactttc ttattatatc gtttttttaa
    32341 tcttgcgtca actggaaatg atctccgttg aattagacca attccctaca tctctgatta
    32401 cttttccagg atatattttt tcttactgcc gtttttcttt ttatcccaaa ttttcatatt
    32461 actaaacaca gctactagaa tatttccaaa tataaaggtg cctatcaccc aatatatgga
    32521 ctcagttgtt aggtattgtc gatccaagcc atcctttaaa tggaatagta tagcagtttg
    32581 gttaacaatc ataaaggttg gccagaaact tttgttgaaa aaagtagaca ttttcattat
    32641 ttgttgccgc tttctgtaag gttaatactc aataaaaatc aaaaagcaaa ctaggaagct
    32701 agcctcaagc tgtacttgag tacggcaagg caacgctgac gtggtttgaa gagtataggc
    32761 ttagtatact actaggcaag caaataagca aataaacaac tagaatagaa aaagataggg
    32821 ctctaaaaac tgacttctat tccttaaaaa cgaaccagct tgactgattc gtcttcttac
    32881 gtttatctcc tacttccgat acattttaaa ctgtaggaag aggtcgctat atttccctgt
    32941 ccatttatgg tcaaatttct cataaatttc taggtgtttc atggtttcaa catcgggata
    33001 gaaggcctta tcttcctttg tttcctctgg gagcaattcc ttcgctggta tgtttggtgt
    33061 tgaatagccg acatactccg cattttggag agcattttca ggtttcaaca taaagttgat
    33121 aaaggcatag gctgagtctt ggtttttaac tgttttggga atgaccatat tgtcaaacca
    33181 aagattgctg gcctctgtcg gtaccacata acgtagattt tcattttttt ctaacatttg
    33241 gctggcttca ccagagaagg tcacgccgat tgcaacgtta ttctgaatca tatagccctt
    33301 catctcgtcc gcaacgatag ccttgatatt tggagtcagt ttgtagagct tatccactgt
    33361 ctcttccaac tgctgcagat ccttggagtt gaggctgtag ccgagggaat tgagtcctag
    33421 tcccagcacc tcacgcgccc catcaaagag catgatagaa ttcttatact ccggcttcca
    33481 aaggtcatcc caatgctcag gcgcttcatc taccatggtt tcgttgtaga caattcctaa
    33541 ggttccccag aagtaaggga tggagaattt attacctggg tcaaaggact ggttgagaaa
    33601 ctctggtccg atattttcga ttccttcaat ttttgaataa tcaagcggaa ccaagaggtc
    33661 ttcgtccttc atcttgttaa tcatgtattc acttggaatg gcaatatcgt aggtcgttcc
    33721 accctgcttt atcttagtgt acatggcttc gttggagtca aaagtctcgt actgaacttg
    33781 aattcctgtt tcttctgtaa actgagtcaa gagttcagga tcgatatagt ctccccagtt
    33841 atagataacc aatttttgac tatctcgact attgatttta ctatctaaat gagtcgcaat
    33901 tccccacaag acaaggataa tcgctgcaat tcctgctaaa aatgaataga tttttttcat
    33961 gcttgctcct ccttctcacg agagataaag taataaccta caactaggat aatactaaag
    34021 agaaagacta gagcagacag ggcattgatt tctaaggaaa tccccttgcg agcacgagag
    34081 taaatctcga ctgatagggt tgaaaagcca tttcctgtta caaagaaggt cacggcaaag
    34141 tcatctaacg aataggtgaa ggccatgaaa taaccagtaa tgatagacgg agtcaggtaa
    34201 ggaagcatga tttccttgaa catctgaaat tgactagctc ccaagtcata ggccgcatga
    34261 atcatgtcgc cattcatttc cttgagtcga ggcaagacca tcaagaccac gataggaatg
    34321 gagaaggcca cgtgactaga tagaacggtc aaaaagccaa gtgaaaactt gagttgggta
    34381 aagagaatca agaagctagc accaatcata acgtcaggcg caaccatgag gatattattg
    34441 agtgatagaa aggcttcttg gtatttctta cgagactggt agatgtaaat ggcaccaaaa
    34501 gtcccgataa tggtcgctat caaggctgat aggaaggcca agaaaaatgt ctgagccaaa
    34561 atcagcatga gtctcccatc tccaaacatg gtttcaaagt gagtccagct aaaacctgta
    34621 aagctattca tatcatcacc agcattaaag gcatagccaa tcaagtaaaa gataggcagg
    34681 tagaggacca gaaagaccag tcccagataa aggttggcaa attttttcat cgttctctcc
    34741 tttccttagt cacccacatg gtgatgaaca tggtcaggat gagaatcaca ccgatggttg
    34801 aacccatacc atagttgtca ttggttagaa aattctgctc aatagccgtc cccaaggtga
    34861 taacgcggtt cccaccaatc aaacgggtca gcatgaagag actcaaactt gggataaaga
    34921 ccgactgaac cccacttcgc acaccgttca tagatagagg gaagatgaca tgacggaagg
    34981 tctcccactt agttgcacca aggtcataac tagcattgat gagattatta tccatgtcgt
    35041 ctaagacatt gaaaatcggc aaaatcataa aggggagctc gatgtagctt gcgacaaaga
    35101 taaaggaaaa atcggtaaaa agcaactgtt gtgaaccaat tccgataaat tccaagaatt
    35161 ggttaataga gccattttga ccaaaaatcc cgataaaagc ataggcttta aggagcaaat
    35221 tgatccaggt aggaaggata atcagcatga gccagagttg acggtgcttg agacgggtca
    35281 aaaagagggc tgttggatag ctgataagca aggtcacaaa ggtcacaatc cctgcataaa
    35341 gcactgagtt gaaactcatt ttgaggtagg tcaagttttg tgacgcaaag taagatttat
    35401 aattttctaa actaaactgg ccttcaatgt tgaaaaagga ttgtccgaaa atcaagacca
    35461 agggtgccaa tacaaagagg gcaatccaaa gcatgtaggg cactacaaag agtttagagc
    35521 ttgttttctt catctctttc ctcctcgatc gcattgatca gacctgcttc ttgctcttcg
    35581 atttccacgt attcttcgat acgagcatcg aactcttctt cggtttcatt gagacgcatg
    35641 atgtggatgt cttctgattc aaagtccaga ccgatttcct cacccacgat ggccttacgg
    35701 gttgagtgga tcatccattc atttccaagt tcgtcatagg cgataatttc ataatgaact
    35761 ccacggaaga gctgggtgtc gaccttaact tggagtttgc cttcttcagg aagggtaatg
    35821 cgcaagtcct ctggacgaat aacgacctca acaggttcat ttggcttcat cccaccatca
    35881 accgcttcaa agcgtttgcc gttaaattcg accaagtagt cctcaatcat ggtacctggc
    35941 aagatgtttg actccccgat aaaggtggca acaaagtggt tgattggctc atcgtagatg
    36001 tccacagggg ttccagactg gacaatctcg ccatcattca taacgaaaat ccagtcactc
    36061 atggcaagag cttcttcctg atcgtgagtg acaaagacaa aggtaatgcc caatcgttgt
    36121 tgtaattcac gcaattcgta ctgcatgtct gttctcaatt tcaagtccag cgctgataaa
    36181 ggctcgtcca acaagaccac acggggttgg ttgatgatag cacgggcgat ggccacacgc
    36241 tgacgttgtc ctccagaaag tttgcggatg gaacgttttt cataaccttc caactgaacc
    36301 atcttgagaa cttccgctac acgctgctcg atttctttct tatcaatttt acgcaagcga
    36361 agtggaaagg caacattttc aaacacattc atatgtggga acaaggcata ggattggaag
    36421 acggtatgta cgtcgcgctt gttggttgga atatcattga tacgaacacc gtctagcatg
    36481 atatctcctg tcgtcgcatc cagtaaacct gcaataatgt ttaggatagt tgatttcccc
    36541 gaaccagatg cacctagaag ggtgtagaat ttcccttctt ccaactcaaa gttgatgtct
    36601 ttgagaacct tggtgttgct gtcttcaaaa actttagaga cgtttttgaa ttcgataatt
    36661 ggttttttca attggcataa attccttctt tttcatagat taaccgatcg gggctctgtc
    36721 aggtccccac tacctcttgc agggagtaaa accacctgca tacatcttcg ctaccgatag
    36781 gctttcaccc aagatccgga cttctctttc aagcgtaata cctgagtgtt ccttgacttt
    36841 ttcgataacc gattggatca agtcctcgta gtctttggcc gttccatctg cgacattgat
    36901 cataaatcct gcatgctttt ctgacacttc tacgccaccg atacgatagc ctttcaagcc
    36961 agcttctgaa attaactgac ctgcaaaatg cccgactgga cgcttaaaga ccgagccaca
    37021 agatgggtat tccaaaggtt gcttgagttc acgtaggtgc gtcaagcggt ccatttcctg
    37081 cttgataacc tgatgggttc ctggagctag ggcaaattta actgacaaga caactgcacc
    37141 agactcctga atagctgaat ggcggtaacc aaaagccaag tctttagcag acagggtttc
    37201 gatttctcca tccttggtca agaccttaca agactgcaag atgtgagcaa tctcgccacc
    37261 ataggcaccc gcattcataa agacagcacc gccaacgctt cctggaatac cacaagcaaa
    37321 ctcaaagcca gttaaactat gacggagggc aatgcgagtt gtttcaatca agttagcccc
    37381 agcttctgct tcaatggtat agccatcaac agaaacgtta ttgagcttgt cacacaagat
    37441 gacaaatcca cgaatcccac catcacgaac gatgatattg cttgcattgc caagaaccat
    37501 ccaagggata ttttcttggt tggcaaattt cacaaggcga gccaactcaa aacgatttcg
    37561 tggaaagacc aaataatcag cctctccacc tacttttgta taactatagc tatgcaaggg
    37621 ttcctgaaaa cggatatcaa ttccttctaa gatttcaagc attttttctc ttacagacat
    37681 gtcactcttc cttttccaaa attcattcca ttataccatt tttagagaca tttgacgacc
    37741 ataaaaatac cttgtttgga ttttgcataa gaaaaagagg ttcccccctt tttatgattt
    37801 tttacaaaag atttccttgg ttccataggc gaccagaacg agctccagtg ctagaatcac
    37861 ttcaaccaag actggatttg tcaaccagcc tacttggaaa agagatggtg ccagatcaaa
    37921 gaaggcatgc aagccatagg ctgttaggag ataaatccat ttcttctggc gaacagcttg
    37981 gtaaacccaa actgtcaaaa gtaattggaa accaagcgcc aagattcgct caaaaccaag
    38041 caaataaatc tgccagaccg aaagtgactg aatggttttt aacatatttt cagacagtaa
    38101 ttgcataacc tgtggattct gagtttgaac tgccgaaaga acaatgtaaa gattgagtaa
    38161 actagtaagg cctagaaaaa tcaactccaa gccaccatgc cccagtccat aggccaaggc
    38221 gtctgccttt tccaaacttc tctttttctc aaaccatttg aagaaaacaa gacgagccgt
    38281 ttcctcaaaa aatgctgcca tggctaggcc atagataata tagataagcg gatgatcttg
    38341 caagagggca atactaccgt ctttttgagg gtgcaaaatc aagatatgca ccagtttttc
    38401 caaaatctgc gaagagacaa agaaagcgac tgcacccaag cccaagacag ctagattaat
    38461 ctggtatttc tttttggcaa accaaatgct tcctatcaga aaagctagca acagcaacat
    38521 ggtaataatg atatgaatgg tcattttctt ctcctattct gccttttcaa tatctttttt
    38581 catctcgtca acattgaact tagcaaacaa gtattgacgg tcttggactg ggaaacgttg
    38641 gtccaactgg tcaactgctc ccacctcgat aatgccttcc ttgatgacaa aatccatgac
    38701 atgtccaccg aaggtcaaat catctgatat gaagtgcaga tggtagcctg ccacactgac
    38761 cccatggaaa atctcaggcg tccagaaacc aacaatagtc cccgcaacat tgtcacgact
    38821 atattccggt tgatgggttg cgacatcagc aaacttggta tcgggtgttg acttaggaat
    38881 catacgcaca tgcatatgta aaaattcccc ccgaatcttg atagagcgga aaagattttc
    38941 cccatcataa taagactcaa ttcgttcttt caattccttg tctgtcatct caaagcgctg
    39001 gcggaaaatg acctctgcct gatgcggtac cactgcagcg taaggaataa gggcatctgg
    39061 tgacacttcc acaatttctg gctggtctcc tgaccctttg gcctgataag ccttgccatc
    39121 caagacaatc aattccccat caatggaatc caaggttccc aaaccaaggt caccatgttc
    39181 tagcaattct cctactgtca tagtaccacc ataaaggcca gccatcaagg ctccaagagt
    39241 attgtattga aataatttca ctggttcctg cacgttctta tccattcact ttcttgtctg
    39301 ttattctata aatcttactc ctaagtatac cacatttgcc cctagatgtg aacgagagaa
    39361 acgttctaga cattgccaag aaggaaaaaa aagggtacaa tgtaacaaaa tcaagggagg
    39421 tctggaatga agaaacaaag caagtacaaa gaggtcgttt cctatctgaa aaatggtatc
    39481 gagtctggac gatttccgac gggtagtcgc ctgccttcta tccgtcaact gagccttgac
    39541 tttcactgca gcaaggacac cattcaacga gccctgctgg aattacggca cgaacaatac
    39601 ctctatgcca agcctcagag tggctactat gtattagaac aagggcaaca tcaagaccta
    39661 gaaatcgagg ttaccgacga acatgccagt gcctatgacg atttccgact ctgtgtcaat
    39721 gaaaccttga ttggccgaga aaactacctc ttcaactact atgacaatca agaaggatta
    39781 gaagacctaa gacagtccat tcacaaactc ctctttgagc aagctctcta ctgcaaggct
    39841 aaccaactag tactgacttc tggaacccaa caagccttat ttatcctctc tcaaatatcc
    39901 tttcctagac aagccaagga aatcttggtg gaacagccaa cctaccatcg gatgaatcgc
    39961 ctcttgattg cacaggggct ggactatcaa acgattgaac gaggcattga tgggattgac
    40021 ttggaggagc tggaaggcca cttcaaaaca ggaaaaatta aatttttcta caccattccc
    40081 cgatttcact atcctctggg acattcctat tctgagcaag acaaacgatc tattcttaac
    40141 ttagctgcca agtatgatgt ctatatcgta gaggacgatt atctgggtga tttggactcc
    40201 aagaagggcc aaaccttcca ctatcttgat acagaggagc atgtcattta tatcaagtcc
    40261 ttctcgacca gcctttttcc tgcccttcgt attacagcac tcattcttcc aaatgctatc
    40321 aaagaagcat ttgtggccta caaaaatatc ctagactacg acagcaacct cattatgcaa
    40381 aaggccctgt cactctatat tgacagtcaa ttgtttgaaa aaaatcgttt ggctcgcttg
    40441 accaatcatg aatcttacca aaaacaaatc gaggaaagga taactaaaac accttgtccc
    40501 cttcctcatt atcccctaca cgatggttta ttgctagacc tgagacagta tcctaaaatc
    40561 gccagtctca aacacagtca actgggcttg gacttctttg aagaggccta tttgagcacc
    40621 tgtccttatc aatttgccaa ggtgtcccta gacaatctgg aaaatgtttt aaactattta
    40681 aaagcagaat tggaatgaca tccaactctg ctttttctta ttcttcaact tcagttagac
    40741 ttgctgcttt ttcaagatag ctttggtaag ttccttcatc cttaagtttt tggatgacct
    40801 tgtctaccac ttctttcaaa tcatcactat tttttctaag agcaacggca ttggcgtcgc
    40861 cgtccttcat cttcaagctg acagttgcga cagctaagcc agcgttttta gcagcataac
    40921 taagtgcaac aggctcatcc atatgaacag catctatttt tccagcctgc aattcattga
    40981 ctgcttcacc catattagtt agggaagtta attgagcttt tggcaattgt tccttgacca
    41041 ttgattctgg aacagtccct ttttgggctg caatattagc actttctagg ctagttaaat
    41101 ccttgtattt ttccacatca gccttacgaa ccaagaaact aatcttgttt tcatagtatg
    41161 ggattgaaaa atcaaagact tcttttctct cgtcagtagc actaattcct gcaactgcta
    41221 ggtcagcctt accagtttga agactggtca aaacattgtc aaaactcatg cttaagattt
    41281 ccaacttaac cccaagttca tcagcgatag cctgagccat gtcgatgtct gcaccgacta
    41341 cctggttctt tccatcaacc aatgattgaa attcaaaggg tgcatagtca ggactggttg
    41401 ccacaactaa tttccccttt tgcttaatgg cctcaacagc tgactgagaa ccattagaac
    41461 ctgactggca agctactaag aagaaacttg caagaaaatt acataaaaca aatatccatt
    41521 tttttgattt cataaataaa caacctttct gttaattttg tataattata acgatatcca
    41581 agttacttgt caagtgtttt ttaaattttt atctcaaaaa tattttttcg ttcaaaaaaa
    41641 ggagccatca gttgatttca agctcctttt tatacagaat taaactattt tatagttcga
    41701 caatcttacc tgtttcaaag tagacaaccc attcacagat atttttagca tagtcaccga
    41761 tacgctccaa gtaggaaata acttggaaat aatcacgacc cgtaacaatg gcttctggat
    41821 ttttcttaat ctcttcagtc gcaaggtcac ggatagtttc aaaatagtgg ttaatttgct
    41881 catccatgga ggccacccgg tatgcgtcgt caacagaacc attaagataa agatcaagtg
    41941 ctgcttccac aacgctttta acttcacgtc ccattttttt aatttcttcc tctacagctg
    42001 ggatgcgttc ttcccccttc atacggatgg ttgcctgggc aatggctaca gcgtgatccc
    42061 ccatacgctc cacatctgat acagccttaa ggacagtcaa gactgtacgc aaatcttgag
    42121 agactggttg ttggagtgcg atcatttcaa atgatttctt ttccagtttc acttcgtatt
    42181 catttacttc tgcatcatct tcgatgacct cttttgccag gtcacggtca tgcgtgacaa
    42241 aagcacgtac cgtacgattg atttgtgaga gcacttcttg tcccatagcg tagaactggt
    42301 tatgtaattt ctctaaatct tcttcaaatt gagatcgtaa catctttcat ctccttatcc
    42361 aaattttcct gtaatatagt cttccgtttc cttgtgttgg ggatcaagga acatctgctt
    42421 ggtatcatta aattcaatca aatctccatc taggaaaaat cctgtcttat cagagatacg
    42481 tgaagcttgc tgcatggaac gggttaccag aagcatggtg tacttgtctt ttagaccata
    42541 caaggtttcc tcaattttac cagctgaaat cggatccaaa gccgaagttg gctcatccaa
    42601 gaggatgatt ttaggactag ttgccaagac acgggccacg cagacacgct gctgttgacc
    42661 acctgacaat ccaatagctg aatcatatag acgatccttg acctcatccc agatagaggc
    42721 accttgcaag gctttttcta cggcttcatc cagaacctgc ttatccttaa ttccattgat
    42781 acgaagcccg tagacaacat tctcatagat agtcataggg aaaggattag gttgttggaa
    42841 aaccattccg atttccttac gtaattcaac cgtatctgta cgcggactgt agatgttgtg
    42901 accattgtac accacggatc cagttgtggt cacctctgga ttgagatctc ccatgcggtt
    42961 gagagacttg aggagggttg acttccctga tccagatgga ccaatcaagg ctgtaatttc
    43021 cttaggttgg aaagataggg aaacactatt caaagccttc tttttattat aatatacgga
    43081 caggtctgat acctgtaaaa tcgcatctgt catacggttt cctttctaac caaagtgacc
    43141 agatacatag tcattggtgg actgtagctt ggcattttgg aaaatagttg cagtcttgtc
    43201 atactcaatc aaatcaccca agtaaaagaa gcctgtatag tcacttgcac gagcagcctg
    43261 ctgcatatta tgcgttacaa tgatgatggt aaagtttttc ttgagctcaa acatggtctc
    43321 ttctagttgc atggtcgcaa tcggatccaa ggctgaggct ggctcatcca ttaagaggat
    43381 atctggctta acagagatgg cacgagcgat acagagacgt tgttgctgac cacctgataa
    43441 ggtcaaggct gacttgtgga gatcgtcttt aacctgatcc cagagggcag cctgacgaag
    43501 ggaggtttct acgatttcat ctaggacttg cttatcctta actccagcac gttcatgcgc
    43561 aaaggtaata ttacggtaaa ttgacttagc aaatggattg gggcgttgaa aaaccattcc
    43621 aatgtgttta cgcatttcat aaacgttgat ttctggacgg ttgacatcaa ttccacgata
    43681 gagaatctgc ccagttactt tagcaatatc aatagtatca ttcatgcgat tgagactgcg
    43741 taagtaggta gatttccccg atcccgacgg accaatcaaa gctgtaattt tatttctttc
    43801 aaattgcata tcaatcccct taatggattc atttttacca tagtaaacat ggacatcctt
    43861 agtagaaagg gctacttttt cttcaggaaa ggtaaggata tgcttctcat cccagttata
    43921 tgttgacatg gcttctcctt taggcagcgg ttaatttctt gtgtagatag cttccgaact
    43981 tacgagctcc aaagttaaaa atcaggataa agatcaggag cacagcggca gaacctgctg
    44041 atacaatggt tccatctgga atagtgcctt cactattgac tttccagata tggacagcca
    44101 aggtttctgc ttgacggaag atagagatgg ggctagtcac actgaggata ttccagttag
    44161 accagtcaag agctggcgcc gattgccctg ctgtatagat cagagctgca gcttcgccaa
    44221 agatacgacc agatgccaag acgacacccg ttacaatacc tggaagcgct tctggaataa
    44281 caacatgaac cactgtctcc cagcgagaaa tcccaagagc cagaccagcc tcacgttggg
    44341 tatggtgaac gtgtttcaaa ctatcctcta cattacgcgt catctgaggc aagttaaaga
    44401 ctgtcaaggc caaggcacct gaaatgattg aaaatccata ctcaaactgg actacaaaga
    44461 tcaagtaacc aaagagaccc accaccactg atggtaaaga ggacaagatt tcaatacaag
    44521 tccgcacaaa gttggtaaca ggaccttttt tagcatattc agccaagtaa atcccagctc
    44581 ccatagaaag aggtacagaa ataatcaagg taatgaccaa taggaaaaag gaattgtaaa
    44641 gctgaatgcc aatcccacca cctgcttgaa aagcagaaga ccttccagtc aagaaagacc
    44701 aagagatatg gggcaagccc cgaaccaaga tatagagaat caaggaagcc aagattgtca
    44761 caatgatgct agcaatcgta tagaggacag ctgttgcaag tttatctaat ttcttagcgc
    44821 gcataatttt tctttcctct ttctttcgta atcaatttaa tcacactgtt aaaaactaaa
    44881 ctcatcaaga gcagtaccaa ggccagtgac cagagaacat tattatttac agttcccatg
    44941 acagtgttcc caattcccat agttaatata gaagttaaag ttgcagctgg tgtggtcaag
    45001 gaagttggga taacagctga gtttccgaca accatctgga tagctaaagc ctcaccaaag
    45061 gcacgcgcca tcccaaagac cactgcagtg aaaataccag aacgggccgc cttcaagatc
    45121 acacgccaga tagtctgcca gcgagtggct cccatagcga aacttgcctc acgataataa
    45181 cgaggaaccg cacgcaagct atccgttgtc ataaaggtta cggtcggcaa aatcatgaca
    45241 aagaggacgg aaatccctga caaaatccca aaaccagtcc caccaaagac actgcgaaca
    45301 aagggaacga cgacttgcaa gccaataaat ccgtacacta ctgaaggaat cccaaccagg
    45361 agttcaatag ctggttgcaa aatcttcgcc ccttttggtg atacttcggt cataaaaact
    45421 gctgcaccaa tagcaaaggg tgttgcgata agggctgaga gaatggtaac gataaaggaa
    45481 cccaaaatca taggaagggc accaaattct ttactagaag gattccaagt tcctcccaaa
    45541 agaaagtcaa agatattcac accattgaca aagaaggtcg acaagccttt ttgcgctacg
    45601 aaaaccaaaa tcatggccac aaggatgact atcaaagaaa gacaggcaaa ggtcaaacct
    45661 tttcctaatt tctccagacg agaattcttt gatggaagca acattttctt agctaattct
    45721 tcttgattca ttattgtctc ccttccaaca ctgtcacagt tccggcagca tctttttcaa
    45781 ccttcatttc cttaatcgga atatacttca atcctttgac aatcccttct tgggtctcat
    45841 ccgagagaac aaaattgaga aattctgcag ctaactcatt gggctgcccc aatgtataca
    45901 tatgctcata agaccacaag ggccaattat tgctacttat attttctgga cttaagtcat
    45961 agccattcaa cttcatgctt ttgaccgaat catctatata ggtaagagat aaataagaga
    46021 tagctcctgg actttttgat acgattgatt ttaccgctcc atttgaatcc tgctcctgac
    46081 tttgcatggc agactgacct tccataatga cagtatcaaa ggtagcacga gagccagagc
    46141 cggctgcccg attgataaca gagatgggta agtccttacc accaacctct ttccaattgg
    46201 ttacctcacc tatgaagatt tgacgaagtt gctctgtcgt taggttatca acatcaacct
    46261 ccttattgac aatcagagcc aagccagcta ccgcgacctt gtggtcaaca agagcagaag
    46321 catcaattcc gtctttttcc tcagcaaata catctgagtt tcctatatca actgccccag
    46381 actgaacctg ggacaagcct gtaccagaac ctcccccttg gacattgacc gtttttccaa
    46441 catggatcgt gccaaattca tctgccgcta cttcaaccaa gggttgcaag gcagttgagc
    46501 caacagccgt tatggattct ccacgatcaa tccagctagc acagcctact aaacaagccg
    46561 tcagccaaaa agcgataaga gacagagcaa gcttttttct ttttttcact gtttttctcc
    46621 tcgaaaataa ttatgaatac tgtgaatttt ttaagtagtt ctttatgagt tgacgcatga
    46681 attcttacca aatttctgcg caattgatta tttatataat ataggctata ttactctttc
    46741 ctaacctcct tttttcatat gtggataaaa tctcttgtct atcccttccc ccattgtcac
    46801 ccattatagt catttcgtgt ctctttttcc cctttttaat gcaagggaaa ttactctcct
    46861 tagatgataa tccaaaagct agaaaggtat ctcaaatctc tctactctcc cagactagtt
    46921 tacaactaaa aggaaaagat tctattttat gagaaatcta gtttacaagc ggtaagaacg
    46981 ctaataacta aacttcttgt actctttgaa aatctcttca aagcagtgtt ttgagctatc
    47041 tatggctagc ttcctagttt gctctttgat tttcatcgag tagtaaaact acatgtgatg
    47101 gcaatcaaga tatcaagaat catcctacta aaaaaatcca tactttcact ataacataga
    47161 ataagatatt tgactagcat tttcatttga atctgaggcc ttttggaaaa taatttttca
    47221 aaacatttcc agtaaccttt gcaaagccca agccattgcc tttaaccaaa acttggtacc
    47281 aaccatttgg cagactttct gccagctgaa cggtttctcc agccgcatac ttgacaaact
    47341 cttcttggcc aatttcaacc gactgttcga cctgactcgg tttcaaggct aaaccaagag
    47401 cgaaactggg ctcaaagcgt ttcttcttaa aagtacccag atgcagtcca ttgcgagcaa
    47461 tcttgagctt ccctaaatct ggcaaaagtt ctggcaagag ataaagctgg tctccaaaaa
    47521 tctgcaagat acccggtaga ttgaccttca aatggttttg ggcaaattcc tgccacaagg
    47581 caacttgttc acggctgagg ttactcttac ttgccttaaa tttaggagct ggattgttac
    47641 ccttaaactg tagatgggca acaaactgac cctctccctt aaactgatga gggtacatcc
    47701 gagccgtttc tggcaggtca ataccagcta ccattccatt gatatgctct actggcaaca
    47761 agtcaaaatc atactcttcc agcaaccaat tgacaatctc ttcgttttcc tcgggtgccc
    47821 aggtacaggt cgaataaacc agacgaccac cttcagctaa catggtcact gcatcctcca
    47881 gaatttctct ttgcaagcta gcacattgac tcggataatc taagctccaa tagtccatag
    47941 catcaggttg cttacgaaac attccttcac cagagcaagg ggcatcaaga acgattaagt
    48001 caaaatagcc tttaaagacc ttgaccaagc ggtcggcaga ttcattggtc accacgacat
    48061 ttgtcgctcc aaaacgctcc atgttttcaa ccaaaatctt agcccgtttg cttgaaattt
    48121 cattggaaac aagtagcccc tcccctgcta gataggctgc cagttgagtt gatttgcccc
    48181 ccggtgcagc agccaagtcc aagaccttca taccaggact gggttgggct acttgagcca
    48241 ccatttgagc agcaggttct tgcgaataaa ctaaacctgt agcatgctca ggcgatttcc
    48301 ctgaaacctt cccatagtgg ccccaagggg tttgagtaat ggcatcagaa aaggaaagtt
    48361 gctcttcttt taagggattg acccgaaagg ccgaaaccgc ttcctcctca aaagaggcaa
    48421 gaaaatctct tgcctcatct cctagtattt ctttatattt ttcaacaaat ccttctggaa
    48481 atttcattta agttcttttc ctttcgtaaa tataggactg aatttcctcc tgcatctcaa
    48541 gaggcaccat catgaccggc tgtctggttt gaaaatcagg agcttcacca aaaagggtca
    48601 caacccgata gcccagactt tcccctaaaa tactagctgc ggcataatcc catggttgca
    48661 gataggtgag ataggtcaac aaacgccctg acaaaatctt ggcaaaacta atggccgcac
    48721 ttccatagac acgaacacca agaaccgctc ggctcaaatc agccagcccc cattcattgg
    48781 tttccagcat accactattc cctgcaatga gaaaatctcc aagtggttta gttttaaaag
    48841 aagctagggg cctatcattt agacaaactg gaaattcccc accaccgtgg taacaatccc
    48901 ctttgaccac atcataaatc agaccaaact gtccctgacc attttcaaaa taagccatca
    48961 taacagcaaa atcttcctgc tgggctacaa aattattggt accatcaatg ggatcaatga
    49021 cccaaacctt gccctcttga accgaggctc gcagacaacc ttcttcagca caaatcttat
    49081 cctcaggata acgggacaaa atctcaccaa ccaagagttc ctgaacttct ttgtccagtc
    49141 tggtcaccaa atctgttgga gaggacttgg tttcaacacg caagtcttcc tgcatatggt
    49201 caagaatgta ctgacctgct ttcttaacaa gctctttagc aaattcaaat ttacttttca
    49261 agagaaatct ttcctttccc tttttctttg gcagcctgaa ctgctcggta aagtgaatag
    49321 ccactaaccg tttcaaattc tcgtcccaaa cgtttctctt cgctcttgct tggcacgacc
    49381 gctttgaatc ccttatagga gtccagtaac tttttagcct ctactttgcc ttcataggca
    49441 gcttcaacat cattaaaaaa agaaagcact gaagcaagtt cttcagtgct ccacgacaaa
    49501 tctagtgggt aactatactg tttgttcatt aactaatacc agctctcatt cttgcttctt
    49561 ttagttcttg cttacgataa ctacgaggga gaaaagcacg aatctcatct tcattaaaac
    49621 cgatttgcat acgcttggca tcaataataa ttggacgacg caaaagacta ggatactgct
    49681 caatcaaatg aagcaattcc gataccgaaa tactctctac atcaatattc aatttttgaa
    49741 aaatttttga acgagttgaa atgatgtcat cagtaccatt ttcggtcaag gaaaggatgt
    49801 gttgcaattc ttttcttgtt aaaggactgg tcataatatt gtgttccaca aagggaacct
    49861 tatgtttttc taaccaggtc ttagccttac gacatgatgt acagctcggt gatagaaata
    49921 gtgtaatcat gcttttctct tcttatctat actttgctac ttctattata caaaaaaata
    49981 aggcgcttga ctagggattt ttagaaaaaa agcctatttt ttcaggaaaa ataggctgtt
    50041 tgcgaacgat tgacacaatt ggatttggtt aattcactct taacgatggt tttaaacgat
    50101 atatatattt atatatgtaa attaaaaaca tctttccttt cacttcctac gacttttcag
    50161 acacagatag ccaaagaagc tttcatagag ggcaaaaaag aggaggaagg catgaagaaa
    50221 gaaggtctct ggcaaaatca taataacagg atccttggct ggatcaaaaa gccaggtatc
    50281 atctcccaca aagagaattt gatggaaaag agtaaagaat tggtcaaaac caatcaaaac
    50341 tcccccaagt ccaatcatca caggtaagac tactagagcc aggagacttt ttcgataaag
    50401 agacaaaaag tcctttttca caatcctatt gacaaagaca tagaaacttg gcagtgtcac
    50461 tagagctact agctgaacca aatgaaagag attcttgacc actgcgaaat ggtgcaaacc
    50521 agctgctgac gaacgaaaat caggcatctg taagacctga ctaaaaggat tggtcagata
    50581 attcatcaag atatgaaaat tgtattgaat ggtttctggt tttagataga ctcgattcgt
    50641 taagtttagc cactgaatct ccataggata gaaaatccaa gccagataaa tggtcagaag
    50701 gattgagagg gagaggagaa agagcataga gccccaaaag gtcaatttag ttttcatcaa
    50761 aatcccactc cgcaaggcta gaaaccacat gtgtcggtgc gattggcagg ccagctactt
    50821 cttctgcctt ggtaaaacct gtcgtcacca agagcgttgg aatgccattg tcaatcccag
    50881 cccggatatc agtcaaataa ttgtccccaa ccatgattaa ctcttcacgt tccaaaccta
    50941 agtgctcaac tgccttgtcc ataatgatgg cattgggttt tccgatataa accggcttca
    51001 ctcgtgtcgc tacttcaagc agcgtaatca gtgagccagc acctggcaaa agaccacgtt
    51061 ccgtcgggat gttgaggtca ggattggttc cgataaaatg ggcacccttt tgaatagcaa
    51121 gagttgctgt ggcaaatttt tcatagtcga cttgccaatc cagaccaact accacgtagg
    51181 caggtttttc cttgtcttcc acataaccag ccgccttgat ggcttccttg agtcctgctt
    51241 ctccgacaac atagacggtc ttttcaagcc ccaaatcatt catatagtcg atggttgcca
    51301 aagtcgctgt gtagacagtc gataggggcg tatcgatatt aaaattctga gccaacatct
    51361 ccttaacact ctctggagtg cgggttgtat tgttggttac aaagagatag ggaatgtccc
    51421 gcttttgcaa ttcatgaaca aaagtctctc cagcagggat ttggtctttc cccttataaa
    51481 tggttccgtc taaatcaatt aaatagcctt tatatttcat ctatttctcc ctaagccttt
    51541 tttatttctt gccaagtaat gattgcttgg gcattgataa ccccatcact tgtaatttca
    51601 tgcttgcttt ccagtccagt ccgttcaaca gccgatgtaa tcaccccacc tggtcgaact
    51661 tccttgacat acttgaggtt gattttcttg ggaatatagt gggtcaaaaa atccgctccc
    51721 atgacctcaa aaatccagtc taagtattta ctgttattga catgaccatt catatccaag
    51781 tcgtaaaaac gaacatggta atccttgctg atcgactctt ccaaggactc atacttcggt
    51841 ccacggataa gttttttatc aaaatcagac tggtaaggag ccacaatctc aggttcaaca
    51901 gcatggactt ttcgactgtc gcggtccatg agaacaaagg tcgccatcat atggatgagc
    51961 tcctgccccg cttcattata aatagtaaag cgacggtagc aaaaaagtcg attgtagctc
    52021 aaggcttccg tttcgatggt aatttcttcc gcaaaacgag gcaaacgaac cacctcaata
    52081 tcatattcta cgataatcca gaccagatta tagtcttcca aaatggcctt atcactaact
    52141 cccagttcaa tcgactgcat ccctgaaact tgcagtgaca gcaaaattac atctggaagt
    52201 ttgatatgac cgttcatatc agccatatca aaaggaattt tcattttcat ttgataagtt
    52261 aagcccatga tcctactcca aaataaatcg ttccgctaca gtatctccca aaaagagacc
    52321 tctctttgtc atgcgaacgt ggtcaccctc aatctgcatg aggccttgtt gaaccaaatc
    52381 tctgacaatt tctccataaa gtccagcaaa agactgtcca aatttttcct caaatcgcgc
    52441 catggaaacc ccggatttct tgcggagtcc caagaacatt tcttcttcca tttgctcctt
    52501 ttgactcagg tgatcttctg taatacaagc attgccttcc tcaaccgcac tgagataatg
    52561 acgaatggga ccatgatttt tatagcgtac tccattgaca taaccagatg cccctgcacc
    52621 aataccatag tattcagcat tgtcccagta catgagattg tgacgacttt caaagccggg
    52681 tttggagaaa ttagaaatct cataatgctc aaaacccgct cgctccagct ctgcaatgat
    52741 gtactcaaac atctccgctt ctagttcctc cttaggcaga ggcaatttcc cacgtcgcat
    52801 ccgattcata aagaccgtat ggttttctaa aatcaaacta tacaaactca tgtggggaat
    52861 atccagtcca atggctttag ccacattttc ctttacttgc tccatggtct gaccaggcag
    52921 agcataaatc aaatcaatgg agatgttgtc aaaaccagcc agtttcaggc gatcgatatt
    52981 ttcataaata tccttctcca aatgactgcg cccaatcttt ttcaacatct tatcatcaaa
    53041 ggtctggaca cctagcgaaa cacgattgac agccgaattt ttcaaaacag ctatcttatc
    53101 cgcatccaaa tcgcctggat tggcttcaat ggtcaactct tctaagacag acaagtccaa
    53161 gtttttagtc aagccattca gtaaaacctc cagttgcgga gccgacaggg ctgtcggtgt
    53221 tccaccaccg atataaaggg ttgacaactt ttcaatatca taagaacgaa actcttccag
    53281 cagatgctct aaatagctgt cgactggctg atttttgatg aagacctttg aaaaatcaca
    53341 ataataacaa atctgggtac aaaatgggat gtgcacatag gctgacgttg gttttttctg
    53401 catagtaatt attataccac aaagactaga ttccagataa aaatcaccat ccccagatac
    53461 atagtccgtc cggagatggt gatggtttat tcttctgtta tatcaatcac aatctcttct
    53521 gagtcatcaa gagcttcggc tttttcttgc cattgctcct tgagattatt taattgattt
    53581 tttgatgctt ctgtcgcttg aaaagcatag gatttagctt gagcaagtat actgtccaca
    53641 gtgatttcac ctgactcaac ctgttctttt gttttcagaa caaaatctgt agcctgctcc
    53701 ttaacttctg tcagtttttc acagacttgc tccttggcat actccggatc ttctctcaaa
    53761 tcatctagaa aatcttgagc ctgactgcaa acttgtttgc ccttatcact tgttaaaaac
    53821 aaggcaagag ctgcacctga aacggttcct aaaaggattg aggataattt acccataagg
    53881 attctccttt tttatttttt gaaaaattta cttgcaagac gaagagctga cagacttgca
    53941 ccagtcttga gtgtttttga accagctgat gaagctttct tgctcaagac acgcgcatgg
    54001 tcattgaggt ctgaaacaga tagagataaa tctgcaacag cactgaagag tggatcaatc
    54061 gtagccacct tgacattgat atcatctgcc aagacattga ccttagccaa caactcattg
    54121 gtgtgatgca aggtcacatc cacatctgaa gtcaaggttt taatcgtctt ttctgtttca
    54181 tcgatgacac gaccaagctt ttgtacagta atgatcagat agaccaaaaa gacaatcaaa
    54241 gctagggcaa caagaatata tgcaacttct aacatttagt tttcctcctc tgtaatatag
    54301 taaggggcct tctttcgatt ttgataaata acgatcatta taccgagacc gataaggaca
    54361 actgacagcc attgggacac tcgaaggccg aagaacatga gactatctgt tcgcatacct
    54421 tcgatgacca tacgaccgaa accataccaa atcaagtaaa aggccgtgat atgacctcgt
    54481 ctgagactct tccatttccg tctaaaaatc agaatcaagg caaagccaag cagattccat
    54541 agagactcat aaaggaaagt cggttgacgg tagctcccct caatatacat ctggtcacgg
    54601 ataaagccag gtagataatc cagattatcc actgttgcac cataagcttc ttggttaaag
    54661 aaattacccc aacgccccaa actttgagca atcataacgc taggcgccgc aatatctaga
    54721 aaatcccaag tattgatgag tttacggtca gcaaagatat agagcacaag agccccagtt
    54781 atcaaaccac cgtaaatggc caaaccacca ttccaaatgg caaaaatctc tcctaaattc
    54841 tgactatagt aatcaaatcg gaaaataaca tagtagagac gagctcctaa aatagccaag
    54901 ggaaaggcta ctaagataaa atctaaaata tcgtctggta tgatcttctt tctaggtgct
    54961 tctttcatgg tcaaataaac cgcaagaatc aagcctgtca caatacataa ggcataccaa
    55021 cgaatggcta ggggtcctag ttgaatagca attggatcaa gcattttgca cctcatttcg
    55081 agcgatgaga cttgtcagtc gttcgtcgaa caaacgggtc gcatcaaagc ccatttcctt
    55141 ggcacgataa ttcatggcag ctgcctcaat cacaacagag atattacgac ctgttttaac
    55201 tggaatacga atacgaggaa tggctacgcc agaaacttca agttcctctg cattgtttcc
    55261 aagacgatca aaggtcttat gcgtatcgta attttccaaa tagacagcaa gctgaacctg
    55321 tgaagaatcc ttgacagcac tcgcaccgta gagactcata acatcgataa taccaacccc
    55381 acgaatttca atcaagtgtt tcaaaatttc agctggttca ccccagagag taatctcatc
    55441 cttggcaaag atatcgacac ggtcatctgc taccaaacgg tgaccacgtt tgacaagctc
    55501 aagacctgtc tcgctcttac caattccact atctccctga atcaagacgc ccatcccata
    55561 aatatccatc aagacaccgt gcacactggt acgttctgcc aaacgagaat ccagatagct
    55621 agataactct ccagataaac gactggtagc tgcacggctg gttaaaatag caatcttaca
    55681 ttctctagca gcctttaaca tctcctctgg aaccaccaaa ccacgggcaa caatgactgc
    55741 tggtgtctca gatagaaaca tttttttcaa aacttcataa cggctgttgg aaggcatgct
    55801 gatcagataa gaccactcct tcatccccaa aagttgaatc cgctctggtg tgtagtagtc
    55861 aaaatagcct gtcatttcaa gaccaggtcg cgtaatatcc gctgtattga tttccttttc
    55921 aagcaactct ggttcaccat agacaatatc tagtctaagc ttttcaatca cttcttttac
    55981 taaaaccgac atcatttcct ccttcaaatg agttcttctc tctattatat caaattgtag
    56041 gaggaagttt cagatttttg caggctttgg agtatttatt taaaagaaaa aggactagat
    56101 ttctctagcc tttcattcca attagaattt tttacgttta cgagctactt ctgatttacg
    56161 tttacgtttt acagaaggtt tttcatagaa ttcacgtttg cgtgtttctt gaagagtacc
    56221 agctttagta accgcacgtt tgaaacggcg aagtgcgtcg tcaagagatt cattcttacg
    56281 tactactgtt ttagacattt ttttcactcc cttcaagttc gatatctatt tcattctaca
    56341 cgtaaaagga ataattgtca agcaaaaatg ctgtttaatt ttgatttttt cttccattta
    56401 tacaagagtt gaaagcgttc aatagaacat tgctttttta tttttcgagt aagctaagcg
    56461 cttcagcatc tgcgatgatg gttacatcag ggtgattttg gaggctactt gcaggtaggt
    56521 tctcagtcac tgggccagat actgttccgg caatggcttc tgctttcgac tcaccgtaag
    56581 caaaaagaat aatagacttg gcatccaaaa tgtttttaat ccccattgaa atagcttggg
    56641 ttgggacgtc ttcaatcttg gcaaagaagc gtgcattggc ttcgatagta gactggtcaa
    56701 gttctactag atgcgtttga ctgtcaaatg gagtaccagg ctcattaaag ccgatatgcc
    56761 cattgcgacc gattcccaag atttggaggt caactggatg gtcagccaaa atttggttgt
    56821 agcgttcaac ttcagcttca gcattgtcct taacaccacg aggcaagaaa ctttctttaa
    56881 atggtttttg gttgaacaag ttttcttgca tgaagtaacg ataagactgt ggattgtccc
    56941 catcaaggcc cacatactca tcaaggttga cactggttag atttgaaaaa tcaaggtcac
    57001 tctcaacaat ttccttgtaa aattcaagtg ggctacttcc tgtcgcaagt cctagagttt
    57061 gagctccatt agccaatttt tccttcaaaa tctcaaaagc aacttttcca ccttgaactt
    57121 ggttttcaac tttaataact ttcatctttt catcctccat ttctgtctat atttattata
    57181 tggtatagac caatttttgt caagtgaaaa cgatttaatt tctaaaaaaa gagcgtccaa
    57241 acttgaaacg tgttataatg gataggatta gaacttagaa agaatgaaca gatatatgaa
    57301 tacagctgat tttgatttcc acttgccaga ggaattgatt gcccaaacgc cccttgaaaa
    57361 acgtgatgcc tccaaactcc tcatcgtcaa ccgtgagaca ggagaaatgc aagataaaca
    57421 cttccactct attattaata tgctggaacc tggtgatgcc cttgtcatga acgacacccg
    57481 agttctccct gcccgcctct atggtcaaaa agtggagaca ggaggtcatg tggaacttct
    57541 cctccttaag aacactagtg gagacgagtg ggaagttctg gctaaacctg ccaaacgcct
    57601 caaggtcggt actcgtatca gctttggtga tggccgcctc agcgctgtcg ttacagaaga
    57661 attgacccac gggggacgca ttgtccgctt tgaataccaa ggaattttcc tagaagtctt
    57721 ggaaagtctg ggagaaatgc ctctgccacc ttatatccac gaaaaattag atgaccgtga
    57781 acgttatcaa accgtctacg ccaaggaaag cggctctgct gcagcaccga ctgctggtct
    57841 tcacttcacc aaagaactgc tggcagaaat ccaagctaag ggtgttcatc tagtctatct
    57901 gactctccat gtcggactcg gaacctttag acctgtttct gtggataatc tggacgaaca
    57961 cgaaatgcat tcagagttct atcaactttc tgaggaagct gctgccaccc ttcgctctgt
    58021 caaagaaaat ggtggtcgcg tcatcgctgt cggaaccact tctatccgca ccttggaaac
    58081 tattggttcc aagtttgatg ggcaaatcca agcagattct ggttggacca atatctttat
    58141 caaacctggg tatgagtgga aggtcgtgga tgccttctca accaacttcc atctgccaaa
    58201 atcaactctg gtcatgttgg tttctgcctt tgcaggccgt gaattagtct tagatgccta
    58261 ccaccatgcc atccaagaac actaccgctt cttcagtttt ggtgactcca tgtttattta
    58321 ttgagaaaga atttctctaa atcttctaat accaataaat cgctaagata ttatttcaaa
    58381 gaacatctac aattgaaact ctagctagct gtagaagacg cctagtacat tgaaattaaa
    58441 atgcttcccc ctagcttcga aaatattgcc atagattgcg ttgactctcc aaattgattc
    58501 atctatattt tatttcagct tcctatattt tcttcgctgt ttgtaaatca aaatgcaaga
    58561 cacatgagta gcaccatatt tgttactctt atctgtcctc tcaagagact attatgagtt
    58621 atttcagaat cattcactac tttgaccctg actctcctta gtctcaaaat caaagactta
    58681 tactcttcaa aaatctcttc aaactgcgtc aacgtcacct tggattatat atgtgactga
    58741 cttcgtcagt tctatctaca acctcaaagc agtactttga gcaacctgcg actagttttc
    58801 tagtttgctc tttgattttc attgagtatt aaacaaaaag tgaacaaatc tgaattctaa
    58861 tgtacagaag actaggcttg ttcacttttt tatagtcgct ataagatgac cttatctata
    58921 gctttttata tataattata tataattata tattcagaca tactattatc aattttgtcg
    58981 cagggaggaa tctgttaacg cacccattca ccattatcat tgactctata gccatctata
    59041 cttgtattga ccgctaactc acccgatgca tttacataat accatttacc accaacttgg
    59101 aaccattgat tgactttcat agaaccgttg ctgttgaggt agtaccatga accattaact
    59161 tgtacccaac ctgttgccat ggaaccatca gtattataaa aataccacat accattttct
    59221 tgtttccagt ctgttgttgg agcaactgct ttagctggtt ctactgctac atctgttcct
    59281 tggttagatg taacagatac aggatacgaa ggaatagatg attgctcagg aacaacaact
    59341 ttttcaggtt ctctcgtccc tctccttata cgtcttttta ccatctcttt agtaatttga
    59401 cgagaagtag tttcttcaat tgttccatca cgttcatcta cagtatagat tgtagtaaga
    59461 gtaatttacc aatttctcct acttcttcta cttcttgact tttatcaaga gttgggtcat
    59521 cgagatattc tgtttcgatt ggaatttctt ggacaagaac ttggggcttg gttccttttt
    59581 taacaactct tgtttgagag tcttcttttt gacttaaagt actctcagtt acttgtccac
    59641 tctttccatc tacatcataa gttatcgttg taactgtttt cccattcttt cctagagtaa
    59701 tctcttgctc ctgtcctgca gaaaggtcat tgtctgcttc atatttagta gcacatggaa
    59761 caagaacttc ttcaaccttg cttttagctg gaactttgat aactgtatcc gtggcttgtt
    59821 ttctatcaac agtaacctgt tcggtaacat gaccagtctc tggattaaca tcgtaggtcc
    59881 ttgtcgtagt tacatagcca tcctctccat caattgtaac aggattttca ctacggtctt
    59941 ttgtttcatc tttttcataa cgaattcgcg tacttgaaat tttcttggtt actaccttag
    60001 gtttagtcgc tacttttaca ataatacccc cattgtcagc gtcatcatac tctattccct
    60061 cttctttatc tctagtatca tctctgacat attgaatccc atcagcagca tgaacaaaac
    60121 ttgtattcag attcctccta aaaataaagt tagcccgatt accgcagaac caaaaatctt
    60181 tccgagttta cgtaatgcat agcgcttatt agtattagat tttgccatta catcctactt
    60241 ctagtatagc atcttttcta tcaaacgtta aacaatatac tttatatata aaatagactt
    60301 agaatgatat attgattatt gaactaacac tttaactata tcgtaatcaa tctcatatat
    60361 aaaggattgc agacatctta tctaaataca tgctaatata tttagataca aacattccaa
    60421 cttgataatt ttcactcatc tttcatcatt ccttatacaa ctatgcagta taaatagaat
    60481 agttttctca tcagaatgag actattttaa tattagataa tttgatagaa aaaacatgaa
    60541 taaaattggt tgaatttaat tccctaactc gatagaggat tctattttaa atttaacaac
    60601 agctaggatt acactataaa aagtacatta ccacctgttc ggctggagaa tctagtcgat
    60661 tagctccctc ttagttacaa gcttaaagcg attttttaac taatactaaa ccagattaaa
    60721 gaaggaacta gaacatctgt cttgtactaa tcaatatgct attttaaagt ttcaaataaa
    60781 cttggaatgc taattattat atttacaata aatagttgac ttcgatagaa acagaaaata
    60841 gaattcaaca ttcttcccta cttataccat aataaataag atccgcaaga tagccttttc
    60901 tctctacacc attaacaatg gtttgtagat aggacattcc agccttttcc atgacacgac
    60961 ctgaagctgg gttgagactg gcataacgtg ctctgacctt ttgaaaacct gcttgagtaa
    61021 aacaaaagtc caagatagct ttcaaagtct ctgtcatcat accatttccc cagtaagcct
    61081 tgcctaacac atagccaatt tcacagctta aatcagcctc gtctatctta acgatgctga
    61141 tatctcctat cacttgctct gggttttctt ttagacaaat ggcccattta taatagttga
    61201 gattagtata ggaagcaacc caattgcgaa tcgagtttcg agtgatttcg atatcaggat
    61261 ggggatccca ggtaacatag gtcagattct cagcagatga agcccaattt tgaaacatgg
    61321 cttctgcatc actctccaca aatcttctta aaatcaaacg ttctgtctgt aatatttgcg
    61381 taccgataga tttcatgatg ctacctcact tccttatttc tatgctcgta gtaaaaaggc
    61441 tcaactcagt aacagctaga aaacgagttt gtcttctaat ttcttgacgc agtcgctgcc
    61501 tggtttgaaa tgcgctctag caagcttttt caaacctagt catcgttgca ggggtaagac
    61561 gatgaaatcg aactcttcga gttaccgaat ctgcctcatt ttcttatttc taaactcggg
    61621 gttcaagatc ccccagacct tctctcactt tcttatttca agatgagagg ctgaaaacct
    61681 ccagtggagg tttttcttgc tttttaattt cagggcaaga agctaaaaag gtccccggac
    61741 cttcttcgct ttctcatttc taggctcagg gtaaaaacct ccaatggagg tttttactcc
    61801 caaaagtcat caaatacagt gatgggtaag tggcgtttgt gttggccttt gtgccaccag
    61861 ttttcaatgg tcgcttgagc ttctgggctg attgttttgc cttctaggta gtcgtcaatc
    61921 tctgcgtagg tgactccaag tgcgacttcg tcagctaggc ctggtttatc ttcttctagg
    61981 tctgccgttg ggattttttc ataaagggct ggctctgcgc caagtttctg caagagctgt
    62041 tttccttggc gtttattgag gcggtaaaga gggagaatat ccgcaccgcc gtcaccaaac
    62101 ttggtaaaga aacctgtgat attttccgcg gcgtggtctg ttccaatgac cgctccgcta
    62161 tgggaaccag caagggcata ctgagcaatc atacggcaac gtgccttgat attccccttg
    62221 ttgaagtctg aaacaggact acctgtcgct tcaactgcag ctgtcatggc atcagctgat
    62281 tccttgatat tcacaaccaa gctgacatct ggctggatga aggctagggc tttttgagca
    62341 tctgcttcat cagcttgcac tccgtatggc aggcggacag cgataaattt gtagctgtcg
    62401 tctcccgttt cagctcgcag ttcttccata gctaattgcg ccaaacgccc tgccaaggtt
    62461 gagtcttgtc ccccagaaat ccctagtaca aaggttttta ggaagggatg ttttttcaga
    62521 tatcttttta agaaatcaat agaacgacgg atttcttcct gggcatcaat cactggtttg
    62581 acacccagct cttggataat cgtttcttgc aaactcattc ttcttctcct tcaccaaggg
    62641 cttccttgcg catcttatca atcaagtcca tcttatcttg ccatacatca cgcgccaaat
    62701 ccactggata gtgctgcgga ttgagcacac gcttatactc atcccacaac ttgtcaaatt
    62761 ccttacgggc ataatcctga atgtcagtca aactaggcaa gttgtaaact aatattcctt
    62821 ctttgaagat atccaccaag agaggaacgg catcaaaatt acgaaccgtc ttcttgatgt
    62881 atgtataggt cggatggaac atcttgattt ctgtcatgtc gctaatatcc acaccatcat
    62941 aagtgatata gtcgccttct gacttgcctt tttcacgact ggtaatgcgc cacacctgct
    63001 tcttacctgg cgtagagact ttttccgcat tattagacag cttaatcgta ttgcgcatct
    63061 gaccagtttc atcttcgatt gcaacaatct tgtaaactgc accaagagct ggctggtcat
    63121 aagctgtaat cagcttggta cccacgcccc agacatcaat cttggccttt tgcatcttga
    63181 gattgaggat ggtattttca tctagatcat tagaagcata aatcttagcc tctgtaaatc
    63241 cagcctcatc cagttgctga cggactttct tagaaatgta ggcaatatcc ccagagtcaa
    63301 tccgcacacc cataaagtta atctgatcac ccagctcacg cgccacctga atggcagctg
    63361 gtacaccgat gcgaagggtg tcataggtat ccacaagaaa gacacaattt ttgtgggtcg
    63421 cagcgtaagc cttgaaagct tcatagtcat tgccataaac ctgtaccaag gcatgggcat
    63481 gggttcccaa aacaggaatg tcaaagagct tacccgcacg cacgttgctg gttccattgg
    63541 cgccaccaat cacagctgcg cgtgttcccc agatggccgc atccatttct tgagcccgac
    63601 gtgtcccaaa ctccatcaag ggttcatctt cgataaccga acgaatacga gctgccttcg
    63661 tcgccaccaa ggtctggtag ttgacgatgt tcaaaagagc cgtttcgacc aactgacatt
    63721 gggctagagg tccttccacc tgcacaatcg gttcattagc aaaaaccaaa tccccttctt
    63781 gggcagaacg aacggtcaac tccaacttga aattgcgaag gtaatccaag aacgccccat
    63841 gataaccaag cgactccaaa taggctatat cactatctga aaaacgcaag tcttcaagat
    63901 agttcacaat tctttccaaa cctgcaaaaa ccgcatagcc gttcttaaaa ggctgttggc
    63961 ggaaatacac ctcaaagacc gccttcttat tgtgaatccc ttggtcaaag taaacctgca
    64021 tcatgttgat ctggtacaag tccgtgtgca atgtcaaact atcatctgga tacatacttt
    64081 tcctacttcc ttagctagaa acccatgaaa attttcaaga actttcatgt attccaataa
    64141 attagtacta ttatatcaca ttttagctgg attgagaaaa gagtaacaag ctattctcca
    64201 ctctccaatt catccatatc ttgttcaaat tttttctgag cccattcgcc atagctctta
    64261 agaccaagat tgccaataaa gacccacgga aggtaaatga cataagtaat gacccaagca
    64321 gacaggtatt taaaattcaa aggattgtgc tgataaattt ctatgttgaa ttgataattc
    64381 tgcaacatca aaagagccgt aatagccaag gttaggaaaa aacaacccaa aatcgtaaaa
    64441 tgaaaacgac tatagtaggt cactcccaga taacgggcac ggttaaaaaa gtaaaatgtc
    64501 cctatgatga taacgattag cagcatatta gaattaaaaa ggcttggtgc taatactgaa
    64561 atgatataag ataggagcga caaagcgatg cagatataga aactttcaga gcccgcttta
    64621 ttgatcagtt gttcttctct ttcgtctagt aattgataat aataaaatct atttttcatc
    64681 ttcttcctcc caaaatagtt ggtctagggt tttccctaaa catctgcaaa tagactggca
    64741 gagcgagaga ctgggattgt attttcccgc ctctatcaaa ccaatagtct ggcgtgtcac
    64801 cccgacagcc tctgccagtt gaccttgtgt taaatcacgc tctacccgag ctaattttaa
    64861 ttttaaattt ttagccacct tcgtcctcct tatagtttta atactcatct actctttaaa
    64921 aatccaaact aacacaagct gtcagctatt tattataacc ccattaattt gcactccagt
    64981 attatcttca gccgaaaatc agtattttag gttgtttgaa ataaacttcc tagtttactc
    65041 ttttattttt atttttattt agcattagct ttagtaggat cttttaaacg agtttttatt
    65101 gcttatattt cttgtttagt ttagttggtt tcatatcact attatataat gcttttggaa
    65161 atgagcctag tataagtcaa aaacgtgttg aatagtgaaa gcaggagcat accaatatga
    65221 ctgatttttt actgtatttt aggtatgcaa aataatacta aatgaaacta aaatgaatac
    65281 ttcttgcttt tcttgagagt gaaaaagtac aataaaatga ttgtagtgat tgccacaaga
    65341 tactgactaa tagacatcgt atttgaagcc acaaatacag gtaaagaata aaaaggaaga
    65401 agttcaattg ctttgataaa caaatcatta ctagtattca tcatcgtatt taatatatta
    65461 gggataaaga gtagtaaagc tactaaaata ccaagccact tttcaatttg tataaataga
    65521 aaatgtaaga taatagaaaa taaaacagga ctaacaaaat tgtattgact acaatagcta
    65581 ctataaaatc taatcctaaa tcacctggaa tacccaattt acttactatg actagtaaca
    65641 caatgccatt caaagcataa ataatggaag ctgagatggc atatagaaaa ttagacaaga
    65701 cgtaaatcat ccgattcttc tgttgattat aaaacaaact taccgtattg tgggaaaaat
    65761 ctctactaat gactcgatta gcatgtataa cagccattaa cataccgagc atagcataaa
    65821 agtttgaaat atgcttaata ccaagcgatg tcccttaagt ctttagaata aagaatgtaa
    65881 gcaatatcgg cggtataaac gagataagca aactgagata tattacgcct gatgaatata
    65941 aatttcttat attttcctta agaaaattca atttatttaa tttcatgatt ataaccttcc
    66001 tttattttga acgatttagg taaatatctt taagcgtctc ttttttagtt tcaaaatcaa
    66061 ctactttaat agagttttcg ttaaaaaatt taaaaagctc actactttga atattcccag
    66121 acatagtaat tctcaatccc tcttcctgaa caatatcccc aaattcttgt ttggtaatga
    66181 aaatgtctct atctgtagct gatgaaaaag ctatctcaaa caagaaatta tgactatctt
    66241 ttcctacttt ttgaaatgtc aaaagcccgt tctccaagaa aagaactctc tcacaaattt
    66301 cttcaatgtc ttctaattta tgactcgata ttaaaattcc cacgttttca tttaaagcta
    66361 atttttttag aaccgctaaa actatttgtg atgattcaat atctaaacca ttagtcggtt
    66421 catctaaaat caatatatca ggttccgtaa cgagagttag aagcaaagct aatttttgtt
    66481 ttgtacccaa agaataggtc tttacttttt tattaataga ctgagtcaaa tctaactctt
    66541 ggatcaaact tctaaatctt tcttgattgt agtcaacacc atataaattt gacaaatatt
    66601 ttaaattctc taaacctgtt ttagataaaa ataattttgg ttcttcgatt aaatacccaa
    66661 cattatcact gcttataata ttacctgaag tcggttgatt attttgaaca agaattttca
    66721 tcaacgtact cttaccaact ccatttctcc ctactagacc aacaatttca cccttgttaa
    66781 gtgcaaaatt tatatctttg agaatgattg aatgaccata atgcttactt acatttttat
    66841 tcctacatta atattcatgg tttactcttt gattcttatt gagtttaaaa agttgcgcct
    66901 ctttttaaca atattatatc atatatctaa cttgatgcaa tatataaatg tcattttgta
    66961 aaattttatt aacaaaaaac tctctacaac aaaataatag agagttttct gcttattaat
    67021 ttttttcaaa atatctaaga catcgcttct ttgtttaatt tgataggctt tgatacctaa
    67081 gctccgactt tatcacttaa aaaataaaaa agacagctaa tctcttgcgc atcaagggat
    67141 ttcgccattc tttaatctcg tttaagtgtt gtatatggag ctttgcataa ttttttaaaa
    67201 ctaaacaact acattaagca cgaactgtga cgctggttcc atcttcctta taaagattga
    67261 taagcccctc tttaagagct cgaaccatgt ctcctgattg aacgggttgt gggactttaa
    67321 tagtcaatag ttccattgga tttggagcgc ggtcgatttt attgcctttg gcatcatgca
    67381 aatcttcaat ataggtttca aaatgacgga aacctggacc ataaaactca acttggtccc
    67441 cttcgttaat gacgtttcgt tgacgaatag ttgctgtttg tgccgcatca tcataagaaa
    67501 ccacttcagc gacaaacttg tactcaggga ttttacgacg agcaccaaac aactgctcat
    67561 tttcagatgg tgtaccatag taaaatcctg tagccagttc acgttgggca accttccaca
    67621 tctcgtccac caagtcttgt ttgatagctt caaacttttc aggactttca agataggcat
    67681 ccacaaccgc cttgtagcag ttggttactg ttaatacata gtgaatagac tccatacgtc
    67741 cttcgatttt tagactgtcc acaccatttt caatcatatc tggaatgtgg tcaatcatag
    67801 acatgtcaac ggctgacatt gaaaattctt ctggaatctc accctgcaaa ctcttacgtt
    67861 ctttcccaaa tggcatatcg taaaggtcgt atttccaacg gcatgactga gaacatccac
    67921 cacggttggc atcacgcata ctctagacgc aattctaatc ttttagactt tgcataataa
    67981 ctgttaaaca gttgaaaatt aacccagttt taacaagtag tcaaaatacc agataaagga
    68041 aaaaaagtct ttaaaatctt aaaaacgcat agtatcaggt attgaacaat cttgatatta
    68101 tgcgttttat tgtgggaaga tttacttcat tttctcttgg aattgagttt tctcctactt
    68161 caaaactaga tgatttgcta tttaaaatag cgttccttat gattttggca acctggatta
    68221 aagcgagaaa aacaaaaggg gcaacttaag ctttctttat attgcttata tgttagtagt
    68281 tttagacaaa caccacataa aataggctta tcctgtatca aagataaggg atacggctca
    68341 aaagggtgat tttctaatga atcatgacac cgataacaag catagtactt tttacaaaca
    68401 tagcattgta aagcaataat atccagcttg ctatggtaat gaatgcatct tgtttgctta
    68461 tccactaaga aaccctgtgc ctctatcatt tccttttcct ttcttaagct cgaactgtga
    68521 cgctggttcc atcttcctta taaagattga tgagtccttc ttttaatgca cgaaccatgt
    68581 ctccagcttg aacaggttga ggcaccttaa tagtcaatag ttccattgga tttggagcgc
    68641 ggtcgatttt attgcctttg gcatcatgca aatcttcaat ataggtttca aaatgacgga
    68701 aacctggacc ataaaactca acttggtccc cttcgttaat gacgtttcgt tgacgaatag
    68761 ttgctgtttg tgtcgcatca tcataagaaa ccacttcagc gacaaacttg tactcaggaa
    68821 ttttacgacg agcaccaaac aactgctcat tttcagatgg tgtaccatag taaaatcctg
    68881 tagccagttc acgttgggca accttccaca tctcgtccac taagttttgt ttgatggctt
    68941 caaacttttc agaactttca agataggcat ccacagccgc cttgtagcag ttggttactg
    69001 ttgatacgta gtgaatagac ttcatacgtc cttcgatttt tagactgtcc acaccatttt
    69061 caatcatatc tggaatgtgg tcaatcatag acatatcaac ggctgacatt gaaaattctt
    69121 ctagaatctc acccttcaaa ctcttacgtt ctttcccaaa tggcatatcg taaaggtcgt
    69181 atttccaacg gcatgactga gaacacccac cacggttggc atcacgcata ctcatgtggt
    69241 ttgaaagagt acaacgtcca gagtatgaaa tacacatagc tccatggaca aaggcttcaa
    69301 tttcaacatc tgtacgtttg cggatttcag ctaattcttt cattgaaacc tcacgtgcta
    69361 aaacgacacg agtcaagcct agctctttcc agaactcaag ggtttcatag ttagtggcac
    69421 tggcttgggt agaaaggtgg atttcaaggc ctggtgcttc agtcgctgca atcataatca
    69481 aggctgggtc agatacgata actgctgcaa tcccgatatc acgcagttta cgaaaccact
    69541 caccagcacc agcttcattt ccttcgtgca taaccatatt agccgctaca tagaccttgg
    69601 caccatactt ggccgcaaac tgcacgcctt cttccatctg ttcgaaagta aagtttcccg
    69661 cacggctacg aagaccatag gcctgaccac cgataaagac agcatctgct ccatactgaa
    69721 cagctacctt tagcttctct aaagtccctg caggtgataa aacctcagga cgttttaatg
    69781 tttttgtcat gatttctcct atttgcaata taaaagtttg acatttttcc ttctcatttt
    69841 atagtaaata aaggataaaa tcaagcctag acggattgtt ttgacaattc taatctttta
    69901 gactttgcat aataactatt aaacagtcga aaattacccc agttttaaca agtagtcaaa
    69961 aagtctaaaa aagtaaaaac cagtatccca agatactgac tcgtaaaaat atataaatta
    70021 gtccttagat gactactccc actctgttgc tcctataatg gaaaaggatt gatacagtct
    70081 agaatatcgc ttttttattt attattctga tataaaactg ttagaattga ttttttgttt
    70141 tatacccaaa tatgaataga gacgtagcta ataagttgaa taacatattt ggaaaaatga
    70201 gaaatagcaa tattactaga acttttagaa acaatcaata tttaaaaaga aaaaagctca
    70261 aagaatttat taaaccatgt tataatagat atagctataa ttttatttat aacaagagga
    70321 tagaaatgac cgaattagaa agaaaaaatc gaaaaattag ctaagaaata ttctgataac
    70381 ttaaacatca aagttcaaga gagagttcgt gaaatggcaa atgataataa gagccattat
    70441 ttgatataca gagttttagg tatttcattt gaagaaggag aaaatatcga tttgtatcaa
    70501 aataaaggtc gttttttata caaatatgct ggttcatttt tagaagaagc tgcagtacta
    70561 tcctttaacg aaaaatttgg tacagaaaat acttaaaaag ttaacattcc taattctgaa
    70621 aatacaaaac ctaagacttt tgaaattgat tgtttagtcg gagaaaaaca cgcatacgaa
    70681 ataaaatggt gggatgcaac tacagatgga gaccatataa ctaaagaaca cactagaata
    70741 aaagttattc ataacaaagg atatatacca attcggttaa tgttctacta tccaaataga
    70801 actcaagcta taaaaattca gcaaacttta gaaacattgt ataacggtat tggagggaaa
    70861 tattattatg gagattctgc ctgggaacat ttaagagcag tgaccggtat tgatttactt
    70921 agtattctaa cagatattgc aaataaaaaa acaggggtaa aatcaaaatg acagtattaa
    70981 aaggagataa cttagaaata ttaaaaacta ttgaatcctc aagtattgat ttaatctata
    71041 tggaccctcc tttctttaca cagaaaaccc aaaaattatc taataacaaa aatattatgt
    71101 attcattcga agatacgtgg acttcgattg aggattacaa agaatttttg tctgtaagat
    71161 tagaagaatg caaaagagtg ctaaaaaata gtggcagtat tttcgttcat tgtgataaaa
    71221 ttgcaaatca tcatattaga ttaattttag ataatatctt tggagcagat atgtttcaaa
    71281 gcgaaattat atggaactat aaacggtggt ctaattcaaa aaagggatta ttgaacaatc
    71341 atcaaaacat ttacttttat tcaaagtcaa aagattttaa atttaataca atttttacag
    71401 agtattcttc tactacaaat atcgaccaaa tactagtaga acgaaaacga aatggaaact
    71461 ctaaaactat atataaagtt gataataatg gtaactatat tctagcaaaa gagaaaaatg
    71521 gagttcccct ttcagatgtt tggaatatac catttcttaa tccaaaagct aaagaaagag
    71581 taggttatcc tacacaaaaa cctattctgt tattagaaca aattataaag attgctactg
    71641 ataaaaatga tatagtttta gacccgttct gtggaagtgg aactacttta gtagcctcca
    71701 agattttgaa tagaaattat atggggattg atttatctga ggaagctatc aatataactc
    71761 agcaacgtct ggaaaatgtt ataaaaacaa gttcaaattt attgaataaa ggaatcgaag
    71821 catatagaac caaaactgag gaagaggaaa acattcttaa attattacag gcaaaaattg
    71881 ttcaaagaaa taaaggaatt gatggttttt tacctaaaca ttttcaaaaa aaaccgatac
    71941 ctataaaaat tcaaaaaaat aatgaatgtc tgaatgagag tatctcttta ttacagaatg
    72001 ctataaactc caaaaaactt gattttggag tagttataaa aactcattca gataatttat
    72061 tatttgattt tgatacgatt cctgaaaata taatagtagt tgaccatttt gaacttacaa
    72121 tcgaaaaatg gttaagtaaa agtcaacagt tactatagtt tgcaaattat ttacagaaag
    72181 aacaaaaaat gctctgaaac actgatttca aggcattttt tgttagttta aaattactcc
    72241 cattcttctt ttccaaacgt acaatatatc caaaaccatt caaaatacta gattctattt
    72301 tttataatat cactaaatcc acctaattat aggacgtttt cagattttta gtcccgtccc
    72361 agtcccagta ccggagaaat attgttttaa tataatatct ctttttgtct tctaagctct
    72421 taaaagcaaa agaacaagta aagagtcaag acaaggataa aaagtccata ttagggcaaa
    72481 taaaaagctt taagacagat gacaaatcta agtcaaataa gaaagaccat agcaaaggtg
    72541 cagagagata aatattggcg gtcttcggac tgcctttatt tttttatcca tttttcaaat
    72601 caaatttatt cagactatat atgcacatat acacttaaat tcatataaaa acatggcttg
    72661 taaaaaatta ctttaatcac aataatcgca tttaaaattg tgatgtttgc aagctaaatt
    72721 acggacttca cttggaagtt ttcccttgta tcttttataa tagatagaaa atttgctggc
    72781 agatgaatat ccaacagatt ctgctatctc ttttataggt agttcagtgt ttaaaagaag
    72841 agtttcagct acattcattc tttttctttg agtgtactct gtaatgcttt gacaatattt
    72901 ttccttaaat aaatttttta atttagtacc actcatttta gatatttttt caagcgtgcc
    72961 ttgatttaca ttcgttgcaa aatgatcatc taagaatctt gctacatctt caagtgcttt
    73021 atcatcatca atttcaattt tatatttttt tctatttaag tatgtgtcaa ttactatact
    73081 tatccattca tttgcctttg ctttaaagaa aaaatcagcg gcaggagcgt ccatcttaca
    73141 atttaatatt tccattgcca ctctttctaa ggcctttgta agtattattt gattcggttg
    73201 aagcaaggtt gaataaaaag attctggatt aatgttaata gatgctaaat gtttttctat
    73261 tagctctttt ttaaaaccaa tggaaacagc aagataacaa caattctcgt gtaataaaaa
    73321 aacaaaatca tcttttatat tatcaaaatc aaaagtacat aaagagtttg cggtaatagt
    73381 ttgatacgga ttaaactttt ctccgtttgc actgacaatg taacttgaat aaattgaaac
    73441 atagtctgac atactataag tgctattttg aactacttcc tctttgatat aaaaatcatg
    73501 tatatcgata atgaagctgc ctccttcata aaaccaatat aggccttctg cataagttga
    73561 attctctttt ttccaacaaa atgtatgacc tgcacttgaa tacttctcat tgtttttaca
    73621 ttcatctact ttcatataat cttttacaaa atcataatat gacataacac actatccctt
    73681 ttagacaata ttccaattag ccttattaat tcaaaactat tgtattagta attataacag
    73741 atgtataata gaaaagcaat gatagatatt atcaattaag cgaatttata tctaaaaggg
    73801 atattaaaga aaggagatat gcttatgaag atttacaaaa aactatttgc ttatgtccaa
    73861 gataagaaat atcttggggt tttggccata attttttctg ctatatctgc tgtacttaca
    73921 gtatatggat attatttaat ctacaaattt ctagataagt taataattaa ttcaaactta
    73981 tccggtgcag agagtatagc attaaaatct gttattacac taacaagtgg agcgatattt
    74041 tattttgtct caggaatgtt ttcacatatc ttgggattta ggcttgaaac aaatttaaga
    74101 aaaaggggaa tcgatggtct ggaaaaagca agttttaggt tctttgactt aaatccatct
    74161 ggtcaaataa gaaagatcat agatgataat gcagcacaaa ctcatcaggt ggtagctcac
    74221 atgattccag atagctctca ggcaatagtc acacctatac ttgtccttgt acttggcttt
    74281 atagtaagta taagagttgg cataattttg cttgctctta ctataattgg tggcttaatt
    74341 ttaggggcaa tgatgggcga gcaagaattt atgaagatat accaagaatc cctatctaaa
    74401 ctaagtgctg aaactgttga gtacgtgaga ggaatgcaag ttgtaaaaat atttaaagca
    74461 aatgtagagt cttttaaaag cttttataag gcgataaaag attactcaaa gtatgcttat
    74521 gattattccc tatcttgtaa aaggccttat gttttgtatc aatggttatt ttttggactg
    74581 attgcaattt taattattcc tatagtttat tttatgacta gcttagctag cgcagaggtg
    74641 attttacttg agcttatcat gattttattt ttatcaggag ttctctttgt ttcattcatg
    74701 agaatgatgt ggtactccat gtatatttct caaggaaatt atgcagtaga tactttagag
    74761 gcgctttacg aagatatgca aaaagacaaa ttagtgcatg gtaatgtcaa taattttaaa
    74821 aactataata tagaatttga gaatgttagc tttgcttata atgataaagc tatcattgaa
    74881 aatttatcct ttaatttaga agaaggaaag tcctacgcac ttgtcggttc atctggatca
    74941 ggcaaatcaa cagtagcaaa acttatatca ggtttttaca atgttaataa aggaagcata
    75001 aagataggcg ggatagcaat aagtgaatat tctgacgaag ccttaattaa agccatttcc
    75061 tttgtttttc aagattcaaa attattcaag aagagcattt atgataatgt agctttagct
    75121 aataaagatg cgacgaaaga tgacgttatg agagccttaa aattagcagg atgcgattta
    75181 atattagaca aattcccaga aagagaaaat acaatcatag gctcaaaagg tgtttattta
    75241 tccggtggag aaaaacaaag aattgcaatt gctagagcaa ttttaaagga ttccaaaatt
    75301 attattatgg atgaagcatc agcatctatt gacccagata acgagtttga attgcaaaaa
    75361 gcttttaaaa atcttatgaa ggataaaaca gttatcatga ttgcacacag gctatctaca
    75421 attaaagacc ttgatgaaat tattgtcatg gatagtggaa aaattataga aagagggtct
    75481 gacaaagaat taatgtcaaa agatacaagg tataagagcc tgcaagagat gtttaacagt
    75541 gcgaatgaat ggagggtttc aaatgaaaga gttttataaa aaaagatttg ctcttacaga
    75601 tggaggagca agaaatttaa gtaaagcaac actggcttca tttttcgttt attgtataaa
    75661 catgcttcct gccatattac ttatgatttt tgctcaggaa gttttggaaa atatgggcaa
    75721 aagcaatggc ttttatatag tattctcagt tttgactttg atagcaatgt atattttgct
    75781 ttctatcgaa tacgataaat tatataacac aacctatcaa gaaagtgcag atttaagaat
    75841 aaggacagcg gagaatttat caaaattacc tctatcttac ttttctaaac atgacatttc
    75901 cgacatttca caaacaatca tggctgatat tgaaggcata gagcatgcaa tgagccactc
    75961 aataccaaag gtgggcggca tggtactgtt tttcccatta atatctgtaa tgatgctagc
    76021 gggcaatgtc aagatgggtt tagctgtaat tattccatct attttaagct ttatatttat
    76081 acctttatct aaaaaatatc aggttaatgg acagaataga tattatgatg tcttaagaaa
    76141 aaactcagaa agctttcaag aaaatatcga aatgcaaatg gagattaaag catataattt
    76201 atcgaaggat attaaagatg acttatataa aaaaatggaa gatagtgaga gaatacactt
    76261 aaaggcggaa gtaactacaa ttttaacttt gtctatatct tcaatattta gctttatatc
    76321 tcttgctgtt gtgatatttg tcggcgtaaa tctaattatt aataaagaga taaattctct
    76381 ctaccttata ggatatttac tagctgctat gaagataaaa gactctttag atgcatctaa
    76441 agagggcttg atggaaatat tttatttatc gcccaaaata gaaagattaa aagaaattca
    76501 aaatcaagat ttacaagaag gcgatgacta tagcttaaaa aaatttgata ttgatctaaa
    76561 agatgttgag tttgcctaca ataaagacgc aaaagtttta aatggtgtaa gttttaaagc
    76621 taagcaggga gaggtcactg ctttggtagg tgcaagtggc tgcggtaaaa caactatctt
    76681 gaaacttata tcaagacttt atgattatga caagggacaa atcttaatcg atggcaaaga
    76741 tataaaagaa atatcaacag aatctctttt tgataaggtc tctatagttt tccaagatgt
    76801 ggttctcttt aatcaaagcg ttatggaaaa tattagaatc ggtaagcaag atgcaagtga
    76861 cgaagaggtt aaaagagcag caaaacttgc aaattgcaca gattttatag aaaaaatgga
    76921 taaaggtttc gatacagtta ttggtgaaaa cggagctgag ctatcaggag gagaaagaca
    76981 aagattatca atagccagag ccttcttaaa agatgcgccg atattgatct tagatgagat
    77041 aacagcaagc cttgatgtta acaacgagaa aaagattcaa gagtctttaa ataatttagt
    77101 taaagataaa actgttgtaa tcatttcaca tagaatgaaa tccatagaaa atgcagacaa
    77161 gatagtagtt cttcaaaacg gaagagtaga aagcgaaggt aagcatgaag agcttttaca
    77221 aaaatcaaaa atttacaaaa atttaataga aaagacaaaa atggcagaag aatttattta
    77281 ttaggaggac tacaatggat aataaaaaat taaaagtaaa agatttagta agtatcggtg
    77341 tttttggcgt aatttatttt gccttcatgt ttggagttgg tatgatgggc ttgattccaa
    77401 tattgttctt aatatacccg acagtattag ccatagttgc aggaactgtt gttatgttat
    77461 ttatggctaa ggttcaaaag ccatgggcac tatttatatt tggtatgata tcaccacttg
    77521 tgatgtttgc agctggtcat acctacgtag ttgtggtttt atcacttata gtaatgataa
    77581 tagcagaatt aattagaaag attggtaatt ataattcatt taaatacaat atgctttctt
    77641 atgcaatctt cagcacatgg atatgtagct ctttaatgca aatgctttta gcaaaagaaa
    77701 aatatatgga gtggtctttg atgacgatgg gaaaagatta tgttgatgta ttagaaaagt
    77761 taataactta tcctcacatg gctttaatag ccttaggtgc tttcttagga ggaattcttg
    77821 gagcatatat aggcaaggct ctattgaaaa aacacttttc aaatggatta tattgtgtgg
    77881 gatactttac tccttgccta attttatggt gttatctgaa ttaaacccta tagttaagat
    77941 gtttttgagt atacctattg ttattagaat gtttatttta ccatttatgg cagcaagctt
    78001 tatgataaag acctcggatg taggcgcaat aatttcatcg atggataagc ttaagatttc
    78061 aaagaatgta tccataccta ttgcggttat gtttagattc ttcccatctt ttaaggagga
    78121 gaagaaaaac atcaaaatgg ctatgagagt aagagggata aattttaaaa acccagtcaa
    78181 atatcttgaa tatgtttctg tgccactact cattatatca tctaatatat cagatgacat
    78241 tgcaaaagcg gcagaaacaa aggcaataga aaatccaatt gccaagacca gatacattcg
    78301 cgtaaagata cagctaattg attttgttta tgttttagcg gttgctggac ttattgtggg
    78361 aggcttaata tggttgaaat aaaaaattta agtcttgatt atggtgaaga gcatatatta
    78421 gatgatatat cactatccat agccgaggga gagtgcgtgc tatttacagg aaaaagtgga
    78481 agtggtaagt catctttaat aaattcaatc aatggactag ctgtaaggta tgataacgca
    78541 aagacaaagg gcgaaataat tattgatggt aagaatataa aaaatttgga actttatcaa
    78601 atctcaatgc ttgtttcaac tgtttttcaa aatcctaaga catatttttt taatgtcaat
    78661 acgacattag aattattatt ttatttggaa aatatcggtc ttgcaagaga agagatggac
    78721 aggcgtttga aggatatact tgagatattc ccgataaaaa atcttttgaa cagaaatata
    78781 tttaatctat ccggcggtga aaaacaaatt ctttgcattg cagcttctta tatagcaggt
    78841 acaaagatta tagttatgga tgagtcttca tcgaatttag atattaaaag cataagtgtt
    78901 ttggcaaaga tgctaaagat attaaaagag aaaggcataa gcataattgt tgcagagcat
    78961 agaatttatt atttgatgga catagttgac cgtgtatttt taatagataa aggaaagctt
    79021 aaaaaaactt atactagaag tgaattttta aagctagata aaaatgaatt aaatgcttta
    79081 agtttaagag ataaagaatt aagtaaatta aaagttcctt atttaaaaga aggtggagag
    79141 tatcagataa aaaatcttag ctacaaattt actgatgatg agtgtttaag cttaaaagat
    79201 atttcgttca agcttgggaa aatttatggc ataataggat ccaacggacg aggaaaatca
    79261 acgcttttaa gatgtttaat aggtcttgag aaaaaatcaa aagaagaaat ttattttaag
    79321 ggagagaagc tatctaaaaa agaaagactc aaaaactctt cacttgttat gcaagatgta
    79381 aatcatcaat tattcacaga tgaagtattc aacgagctta gattaggagt aaagaatttt
    79441 gatgaagaaa aggcgaaaat cattttaaaa gatttaggcc tggacgaatt tattgaaagg
    79501 catccgatga gtttatcagg agggcaaaag caaaggcttg caatagcatc tgttatgtgc
    79561 aagacttctc catttatctt ttttgacgaa ccttcaagtg gtatagatta ttccaatatg
    79621 ataaaaatat ctgaactgat taataagtat aaaaccatgg ataaaataat ttttattgtt
    79681 tcccatgata tagaattttt aaatgaagtg gcagatgaaa tttttgaatt gtaaaataga
    79741 gtctgttaaa catgtctata gaactggtaa attgtctaaa aatttaaaag atactaacac
    79801 atattgatat ttattagaaa attaacttca ggatttttac agtatttata aactttataa
    79861 tatgtattta tagtattaat aatttccata tctttaggga aattatttat ttacttacca
    79921 taattaaaga gatgagagat aaatatcggc agtcttcgga ctgcctttat ttttttactt
    79981 aatttaatgc agtaaatcat acgattgaaa ggaatctgcg ttatatgcta taattaggta
    80041 gttaatctat acatttagaa aggtaaaata tagaattgga gaatgatatg ggattttcat
    80101 ataataaatt gtaaccaccc aataacgaag tctattgaaa aatctccaga ttagtgaact
    80161 cacggatagt tcctaatctg gagatttctt atttgcactt ttcttgtaca actttagtcc
    80221 atg
//
LOCUS       DAVBBS010000003        78991 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVBBS010000003 DAVBBS010000000
VERSION     DAVBBS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 78991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78991
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.003"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(48..1937)
                     /locus_tag="VQU32_000156"
     CDS             complement(48..1937)
                     /locus_tag="VQU32_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HET2471688.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFEKDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGGEKALQVGMG
                     ALTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDQAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSLFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2075..3640
                     /locus_tag="VQU32_000157"
     CDS             2075..3640
                     /locus_tag="VQU32_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET2471689.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKKPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMCKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            3640..4404
                     /locus_tag="VQU32_000158"
     CDS             3640..4404
                     /locus_tag="VQU32_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2471690.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEEIAKENLEKVGMGERYWQARPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLILNENQRAN"
     gene            complement(4603..4818)
                     /locus_tag="VQU32_000159"
     CDS             complement(4603..4818)
                     /locus_tag="VQU32_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471691.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNHEKFED"
     gene            complement(5689..6939)
                     /gene="ilvA"
                     /locus_tag="VQU32_000160"
     CDS             complement(5689..6939)
                     /gene="ilvA"
                     /locus_tag="VQU32_000160"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HET2471692.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(6989..7345)
                     /locus_tag="VQU32_000161"
     CDS             complement(6989..7345)
                     /locus_tag="VQU32_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET2471693.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(7583..7819)
                     /locus_tag="VQU32_000162"
     CDS             complement(7583..7819)
                     /locus_tag="VQU32_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471694.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            complement(7926..8948)
                     /gene="ilvC"
                     /locus_tag="VQU32_000163"
     CDS             complement(7926..8948)
                     /gene="ilvC"
                     /locus_tag="VQU32_000163"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET2471695.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVVEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(9014..9490)
                     /gene="ilvN"
                     /locus_tag="VQU32_000164"
     CDS             complement(9014..9490)
                     /gene="ilvN"
                     /locus_tag="VQU32_000164"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET2471696.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(9483..11183)
                     /locus_tag="VQU32_000165"
     CDS             complement(9483..11183)
                     /locus_tag="VQU32_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HET2471697.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(11384..13051)
                     /locus_tag="VQU32_000166"
     CDS             complement(11384..13051)
                     /locus_tag="VQU32_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HET2471698.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(13054..13419)
                     /locus_tag="VQU32_000167"
     CDS             complement(13054..13419)
                     /locus_tag="VQU32_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2471699.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(13576..13764)
                     /locus_tag="VQU32_000168"
     CDS             complement(13576..13764)
                     /locus_tag="VQU32_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET2471700.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(13880..14056)
                     /locus_tag="VQU32_000169"
     CDS             complement(13880..14056)
                     /locus_tag="VQU32_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HET2471701.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
     gene            complement(14167..14316)
                     /locus_tag="VQU32_000170"
     CDS             complement(14167..14316)
                     /locus_tag="VQU32_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HET2471702.1"
                     /translation="MDKVETTIKSLCVHHYNLKFYVFNDDLPREWFQLMEKRLETLNS
                     EIVNV"
     gene            complement(14628..16172)
                     /locus_tag="VQU32_000171"
     CDS             complement(14628..16172)
                     /locus_tag="VQU32_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET2471703.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            16385..16687
                     /gene="gatC"
                     /locus_tag="VQU32_000172"
     CDS             16385..16687
                     /gene="gatC"
                     /locus_tag="VQU32_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET2471704.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEQDNYYIKVPAILDDGGDA"
     gene            16687..18153
                     /gene="gatA"
                     /locus_tag="VQU32_000173"
     CDS             16687..18153
                     /gene="gatA"
                     /locus_tag="VQU32_000173"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET2471705.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDANNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDTKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            18153..19595
                     /gene="gatB"
                     /locus_tag="VQU32_000174"
     CDS             18153..19595
                     /gene="gatB"
                     /locus_tag="VQU32_000174"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET2471706.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDLHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            19724..20284
                     /gene="efp"
                     /locus_tag="VQU32_000175"
     CDS             19724..20284
                     /gene="efp"
                     /locus_tag="VQU32_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET2471707.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            20306..20695
                     /locus_tag="VQU32_000176"
     CDS             20306..20695
                     /locus_tag="VQU32_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2471708.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGVYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            20688..21110
                     /gene="nusB"
                     /locus_tag="VQU32_000177"
     CDS             20688..21110
                     /gene="nusB"
                     /locus_tag="VQU32_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET2471709.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTNVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKVGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(21159..21536)
                     /locus_tag="VQU32_000178"
     CDS             complement(21159..21536)
                     /locus_tag="VQU32_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2471710.1"
                     /translation="MVLSNGQPTFLSTILSFVLLVIIGPVFEETLDRGYFMNTFFPKS
                     KYYLDVILSGLIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDNLRLTILCHSFF
                     NFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(21877..22062)
                     /gene="briC"
                     /locus_tag="VQU32_000179"
     CDS             complement(21877..22062)
                     /gene="briC"
                     /locus_tag="VQU32_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HET2471711.1"
                     /translation="MTGTETFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
     gene            complement(22221..22988)
                     /locus_tag="VQU32_000180"
     CDS             complement(22221..22988)
                     /locus_tag="VQU32_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HET2471712.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(22985..23851)
                     /locus_tag="VQU32_000181"
     CDS             complement(22985..23851)
                     /locus_tag="VQU32_000181"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HET2471713.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(23888..25255)
                     /gene="accC"
                     /locus_tag="VQU32_000182"
     CDS             complement(23888..25255)
                     /gene="accC"
                     /locus_tag="VQU32_000182"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET2471714.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(25267..25689)
                     /gene="fabZ"
                     /locus_tag="VQU32_000183"
     CDS             complement(25267..25689)
                     /gene="fabZ"
                     /locus_tag="VQU32_000183"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET2471715.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIGN"
     gene            complement(25686..26171)
                     /locus_tag="VQU32_000184"
     CDS             complement(25686..26171)
                     /locus_tag="VQU32_000184"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET2471716.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVASEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(26174..27409)
                     /gene="fabF"
                     /locus_tag="VQU32_000185"
     CDS             complement(26174..27409)
                     /gene="fabF"
                     /locus_tag="VQU32_000185"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET2471717.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(27431..28162)
                     /gene="fabG"
                     /locus_tag="VQU32_000186"
     CDS             complement(27431..28162)
                     /gene="fabG"
                     /locus_tag="VQU32_000186"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET2471718.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            complement(28196..29116)
                     /gene="fabD"
                     /locus_tag="VQU32_000187"
     CDS             complement(28196..29116)
                     /gene="fabD"
                     /locus_tag="VQU32_000187"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET2471719.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(29109..30083)
                     /gene="fabK"
                     /locus_tag="VQU32_000188"
     CDS             complement(29109..30083)
                     /gene="fabK"
                     /locus_tag="VQU32_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HET2471720.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            complement(30202..30426)
                     /locus_tag="VQU32_000189"
     CDS             complement(30202..30426)
                     /locus_tag="VQU32_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET2471721.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(30486..31460)
                     /locus_tag="VQU32_000190"
     CDS             complement(30486..31460)
                     /locus_tag="VQU32_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HET2471722.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANNRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(31460..31894)
                     /locus_tag="VQU32_000191"
     CDS             complement(31460..31894)
                     /locus_tag="VQU32_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2471723.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(31988..32773)
                     /locus_tag="VQU32_000192"
     CDS             complement(31988..32773)
                     /locus_tag="VQU32_000192"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET2471724.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            33098..34462
                     /locus_tag="VQU32_000193"
     CDS             33098..34462
                     /locus_tag="VQU32_000193"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET2471725.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            34465..34836
                     /locus_tag="VQU32_000194"
     CDS             34465..34836
                     /locus_tag="VQU32_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HET2471726.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            35050..36324
                     /gene="serS"
                     /locus_tag="VQU32_000195"
     CDS             35050..36324
                     /gene="serS"
                     /locus_tag="VQU32_000195"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET2471727.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGTDEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(36349..37248)
                     /locus_tag="VQU32_000196"
     CDS             complement(36349..37248)
                     /locus_tag="VQU32_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HET2471728.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACSGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPALSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(37358..37906)
                     /locus_tag="VQU32_000197"
     CDS             complement(37358..37906)
                     /locus_tag="VQU32_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET2471729.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(38124..39446)
                     /locus_tag="VQU32_000198"
     CDS             complement(38124..39446)
                     /locus_tag="VQU32_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HET2471730.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGARFIWLYRVFFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(39537..39625)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(39789..42125)
                     /locus_tag="VQU32_000199"
     CDS             complement(39789..42125)
                     /locus_tag="VQU32_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HET2471731.1"
                     /translation="MNKKILETLEFDKVKALFDPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVHFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(42229..42777)
                     /locus_tag="VQU32_000200"
     CDS             complement(42229..42777)
                     /locus_tag="VQU32_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET2471732.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIFYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(42774..43076)
                     /gene="zapA"
                     /locus_tag="VQU32_000201"
     CDS             complement(42774..43076)
                     /gene="zapA"
                     /locus_tag="VQU32_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET2471733.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            43163..44044
                     /locus_tag="VQU32_000202"
     CDS             43163..44044
                     /locus_tag="VQU32_000202"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HET2471734.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQLVEENRGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TSDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLSVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            44048..44662
                     /gene="lepB"
                     /locus_tag="VQU32_000203"
     CDS             44048..44662
                     /gene="lepB"
                     /locus_tag="VQU32_000203"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET2471735.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            44941..47307
                     /locus_tag="VQU32_000204"
     CDS             44941..47307
                     /locus_tag="VQU32_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HET2471736.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLSKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTHLEKIYRQSKESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(47355..48638)
                     /locus_tag="VQU32_000205"
     CDS             complement(47355..48638)
                     /locus_tag="VQU32_000205"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET2471737.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     EDGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(48832..49191)
                     /locus_tag="VQU32_000206"
     CDS             complement(48832..49191)
                     /locus_tag="VQU32_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET2471738.1"
                     /translation="MDSPKSLASYRVIGISDRVLEILKEYLLLEEGDYIFTNNGSPLM
                     ITCFNYFLKNRFRKSEIKKDDFVLTAHVFRYSHISLLAELEVPITAIMDRVDYTNETK
                     ILSVYTHVTEKMKSNIT"
     gene            complement(49490..50119)
                     /locus_tag="VQU32_000207"
     CDS             complement(49490..50119)
                     /locus_tag="VQU32_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET2471739.1"
                     /translation="MVLSKYYGVADGMNVEGRGSANFIKDNVLITAAHNYYRHDYGKE
                     ADDIYVLPTVSPSQEPFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLG
                     TLGLPTSQKNLTGITVTITGYPSYNFKVHQMYTDKKQVLSDDGMFLDYQVDTLEGSSG
                     STVYDASHRVVGVHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGW"
     gene            complement(50164..50403)
                     /locus_tag="VQU32_000208"
     CDS             complement(50164..50403)
                     /locus_tag="VQU32_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471740.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGSNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(50553..50819)
                     /locus_tag="VQU32_000209"
                     /pseudo
     CDS             complement(50553..50819)
                     /locus_tag="VQU32_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(50844..51152)
                     /locus_tag="VQU32_000210"
     CDS             complement(50844..51152)
                     /locus_tag="VQU32_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET2471741.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLRLQIAS
                     "
     gene            complement(51077..51319)
                     /locus_tag="VQU32_000211"
     CDS             complement(51077..51319)
                     /locus_tag="VQU32_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET2471742.1"
                     /translation="MSLADLLEELEAAKDSKKARPMEAYMRHQFSFLGIAVPERNKLY
                     KNIYQKRKKQRLSIGILQTLAGKRSLENTNMWLLTI"
     gene            complement(51320..51952)
                     /locus_tag="VQU32_000212"
     CDS             complement(51320..51952)
                     /locus_tag="VQU32_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2471743.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(51966..52961)
                     /locus_tag="VQU32_000213"
     CDS             complement(51966..52961)
                     /locus_tag="VQU32_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2471744.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLYDVEKTEK
                     FVFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVVDPDLDASFKS
                     LSGKLNLLTEALQKAENHSLDQEEEIIEKERKRIARDLHDTVSQELFAAHIILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFRLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(52958..53656)
                     /locus_tag="VQU32_000214"
     CDS             complement(52958..53656)
                     /locus_tag="VQU32_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HET2471745.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(53734..54744)
                     /locus_tag="VQU32_000215"
     CDS             complement(53734..54744)
                     /locus_tag="VQU32_000215"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HET2471746.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(54728..55735)
                     /locus_tag="VQU32_000216"
     CDS             complement(54728..55735)
                     /locus_tag="VQU32_000216"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HET2471747.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLTPNPDYSLIQETIALMGDFLAVRGQNLRSFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKSEKIIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(55722..56675)
                     /gene="mvaD"
                     /locus_tag="VQU32_000217"
     CDS             complement(55722..56675)
                     /gene="mvaD"
                     /locus_tag="VQU32_000217"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HET2471748.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVIADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YSVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(56657..57535)
                     /gene="mvk"
                     /locus_tag="VQU32_000218"
     CDS             complement(56657..57535)
                     /gene="mvk"
                     /locus_tag="VQU32_000218"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HET2471749.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(57657..57986)
                     /locus_tag="VQU32_000219"
     CDS             complement(57657..57986)
                     /locus_tag="VQU32_000219"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2471750.1"
                     /translation="MTRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMIPHDFPLYLTVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
     gene            58648..58984
                     /locus_tag="VQU32_000220"
                     /pseudo
     CDS             58648..58984
                     /locus_tag="VQU32_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic-modifying protein"
     gene            58884..59471
                     /locus_tag="VQU32_000221"
     CDS             58884..59471
                     /locus_tag="VQU32_000221"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471751.1"
                     /translation="MTILLKLSSTIVYGEIYHYFLQRDTAKESILDYSFAHGYCEIAY
                     ALFAYSKVLEPSMFYNDLHTFHAELKKLLEKVTSNTENLGNLQLSWCEGISGIILYLC
                     MYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVILA
                     CSERDDHGLLMFQGDSGKADLFDFGIGSRGYIGVY"
     gene            59514..60746
                     /locus_tag="VQU32_000222"
     CDS             59514..60746
                     /locus_tag="VQU32_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2471752.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALCIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVHIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLFALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFVTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(60864..61820)
                     /gene="cbpC"
                     /locus_tag="VQU32_000223"
     CDS             complement(60864..61820)
                     /gene="cbpC"
                     /locus_tag="VQU32_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HET2471753.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDDLFDNRPVFEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLRSSGAMA
                     TGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSSGAMATGWYQDGSTWYYLNAGN
                     GDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(61918..62607)
                     /locus_tag="VQU32_000224"
     CDS             complement(61918..62607)
                     /locus_tag="VQU32_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2471754.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVFIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            complement(62619..64043)
                     /gene="gndA"
                     /locus_tag="VQU32_000225"
     CDS             complement(62619..64043)
                     /gene="gndA"
                     /locus_tag="VQU32_000225"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HET2471755.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVSDVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEDQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            complement(64119..65513)
                     /gene="mapZ"
                     /locus_tag="VQU32_000226"
     CDS             complement(64119..65513)
                     /gene="mapZ"
                     /locus_tag="VQU32_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HET2471756.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTNSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLVTQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            complement(65526..66683)
                     /locus_tag="VQU32_000227"
     CDS             complement(65526..66683)
                     /locus_tag="VQU32_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HET2471757.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARLPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVERKLELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            complement(66768..67148)
                     /gene="rnpB"
                     /locus_tag="VQU32_000228"
     ncRNA           complement(66768..67148)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VQU32_000228"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(67169..67498)
                     /gene="gpsB"
                     /locus_tag="VQU32_000229"
     CDS             complement(67169..67498)
                     /gene="gpsB"
                     /locus_tag="VQU32_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HET2471758.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            complement(67568..68095)
                     /locus_tag="VQU32_000230"
     CDS             complement(67568..68095)
                     /locus_tag="VQU32_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HET2471759.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            68161..68757
                     /gene="recU"
                     /locus_tag="VQU32_000231"
     CDS             68161..68757
                     /gene="recU"
                     /locus_tag="VQU32_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HET2471760.1"
                     /translation="MINYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            68754..70913
                     /locus_tag="VQU32_000232"
     CDS             68754..70913
                     /locus_tag="VQU32_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HET2471761.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGIYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(71080..76383)
                     /locus_tag="VQU32_000233"
     CDS             complement(71080..76383)
                     /locus_tag="VQU32_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HET2471762.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATANENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLFSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKTPGNSTWYKGNRVVAPEPGSVNRLSITLKSDGQLNASNNDVNLFDTVTLPGAVN
                     DNLKNEKKILLKAGTYGNDRTVVSVKTDNQEGVKADDTPAQKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVFLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVNDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGQGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLVSDQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALFLASVSLALSALFVVKTKKD"
     gene            complement(76684..78666)
                     /locus_tag="VQU32_000234"
     CDS             complement(76684..78666)
                     /locus_tag="VQU32_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2471763.1"
                     /translation="MKSSKLLALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDDQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQGSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
BASE COUNT        22982 a        17986 c        14610 g        23413 t
ORIGIN      
        1 ttctatcttc cattatatca tactttttca gtctatggta gctctcttta aaaggcaccg
       61 atactgccgc cacctccgcc tccagagaag ccaccaccag aacttccact tccagaagat
      121 acagagtagg tgcttgctgt atttgcgaca ctagcataat ggctcatttg cgcgcttgaa
      181 tgataaaaca gactgtgcca gccataagct acatagagat tgatatctgg attttccact
      241 tgaatctgat gaaccttcat caaatgacta accttgtccg catagccaaa taaggtcgca
      301 tagaccaaga ggcgattcca gaccacaata ctttccagtt cagcctgatc caatcgtgca
      361 atctcacgca acatattttc aaaactggtc cagagatagt agacctcagc tcccgcttca
      421 tttagaacac catcacgatt atctagtcga agcttccaat aatagaaaac agacaaaact
      481 aaccctagaa aaccaagtat tggcaaaggg aggtaaagat agccatgaac gtctaaactg
      541 tacaagaaca aaccaaatcc gataaatagg ggcaggatag tcaaggcacc catacccact
      601 tgcaaggcct tttccccacc agttaaagga cgataataat ctgggagccc ccagaaggaa
      661 actcgttttc tcactccttc ttgcatctgg ttcaatacct cttcaaaaga agatttgagt
      721 tgaagccctc ttgcttgaat ccgtttttca tcagaaactt tggctctacg ataaagacta
      781 tcagatacct tgtaatccgc aaacaaattg gaaagagttt cttctttttt acctgaaaaa
      841 gctagattta ggcagtcttt ctcaaagctt gacaaaccat cttcttttac tagcctcaaa
      901 ccaactgcat ctccttctga aatgatagag acattcccac ggtctatcac atctagcaag
      961 gtagcttgaa taagttgatc aaaggtgaat tttccagctc ccttgaccaa gggactcact
     1021 tcctccaagg aggtcgagta gactgcttct gataaaacca taggctctaa ttccattggt
     1081 ggttcataga gacgatgatt tttggcatat ttgactgaag gagtggtctt tcttctataa
     1141 ataaaataga agcagacact caataacaag gagatagaaa ggatcgaagg gaggacccaa
     1201 gtaacaagtt gtttactctg atctttttct ctaacaatcg agtcttctat cttattaaac
     1261 tcttctaaac gattcccttt caatccctga tccctagcgc tagcaaaatc ggtccgaggc
     1321 caataggcat gcaactcaac tccacgctta gccggaagat tgtctaaacg gatagtataa
     1381 tcaaggttac tcttttcaat cgttccctct ctaaaaagtt tccctgtatg gaaaaagagt
     1441 ttttcagctc ccttgtctcc ccttacatga aattcaaact tttcaataga ctctgaacta
     1501 tctgtcagag gttgccaatt taattcagcg atatcatcat aaaggaaaag taaatttttt
     1561 aagttccaga cgaggtcaac ttcaactatg tcgccctcct gacctggatt atagactctc
     1621 acagtataac catccgcttc ttctgttact tcgctagtca catctgctag ttctgcaccg
     1681 tttttcgcgg cctgaacctt tggatgaggg tcaatgtcaa acccgctagg catcttacca
     1741 gcacgtccaa gtcccacgat ttgtccctta aagtcctcct caaactggta aactatcttc
     1801 tgtctaaact ctgccgtatt atctgcatga atatacaaat ccccttgata agagtttatc
     1861 ttgaaatcaa tggcaaaaac agagagtgga agaaggcaaa acaagcctaa caccagtaag
     1921 aaaaaagttt ttttcatcaa ctaagccccc tttttatctt tcttatcatt atagcatttt
     1981 ttcgtaagta aaggcttact tatgttgaaa aattacgcat ttttcgataa aataggagac
     2041 agttattttt taatagatca gaataggaga aatcatgaga aaaatatact tatctatttt
     2101 cacaagtctc ttgctgatgc taggacttgt caatgttgct caagccgatg aatatttacg
     2161 catcgggatg gaagcagcat atgctccctt taactggacc caggatgatg atagcaacgg
     2221 agctgtcaaa atcgatggga ccaatcagta tgccaacgga tacgatgttc aaatcgccaa
     2281 gaaaatcgct aaggacttag gtaaaaaacc tttggttgtt aaaaccaagt gggaaggtct
     2341 agtccctgcc cttacttctg gtaagattga catgattatc gcaggtatga gtccaactgc
     2401 agaacgcaaa caagaaattg ccttttcgag cagttactac actagcgaac cagttttgct
     2461 tgtcaaaaaa gattctgcct acgcaagtgc taaatctttg gatgacttta acggtgcaaa
     2521 aatcacttct caacaagggg tctacctcta cgacttgatt gcacaaatcc caggtgctaa
     2581 aaaagaaaca gccatgggag acttcgctca aatgcgacaa gctcttgagg ctggtgtcat
     2641 tgatgcttat gtttctgaac gtccagaagc actgactgct gaagctgcga actctaagtt
     2701 caagatgatt caagtagaac ctggtttcaa aactggggaa gaagatacag ctatcgctat
     2761 cgggcttcgt aaaaatgaca atcgtattag ccaaatcaat gccagcattg aaaccatttc
     2821 aaaagatgac caagttgcct tgatggatcg tatgatcaag gaacaacctg ccgaagctac
     2881 aacaactgaa gagactagca gtagtttctt tagccaagtc gctaaaattc tttctgaaaa
     2941 ctggcaacaa ctcttgcgtg gtgctggtat cactctttta atctctatcg tcggaaccat
     3001 cataggtctc attattggac ttgccattgg tgtcttccgt actgctcctc tctctgaaaa
     3061 caaagccatt tatggccttc aaaaactagt cggctgggtt ctcaatgtct acattgaaat
     3121 tttccgtggt acgccaatga ttgttcaatc gatggttatc tactatggaa ctgcccaagc
     3181 tttcggtatc aaccttgacc gtacactagc tgctatcttc atcgtttcaa tcaataccgg
     3241 tgcctacatg actgaaattg tccgtggtgg tatcctggca gttgacaagg gacaatttga
     3301 agctgcgact gctcttggta tgactcataa ccaaaccatg tgtaagattg tcctacctca
     3361 ggttgtccgc aatatcctac cagcaactgg taatgaattt gtcatcaata tcaaagatac
     3421 atctgtattg aacgttatct ctgttgtcga actttatttc tcaggaaata ccgtggcaac
     3481 acaaacctat caatacttcc agacatttac aatcatcgcc gtgatttact ttgtcctcac
     3541 cttcaccgta acacgtatcc tacgctttat cgagcgcaga atggacatgg atacctacac
     3601 tacaggtgct aaccaaatgc aaacggagga tttgaaataa tgacacaagc aatccttgaa
     3661 attaaacacc tcaaaaaatc ctatggacaa aacgaagtgc taaaagacat ttctctcact
     3721 gtccacaagg gagaagttat ttctatcatc ggaagctctg gaagtgggaa atcaaccttc
     3781 ctacgctcca ttaacctact tgaaacgcca actgatggac aaatccttta tcatggacaa
     3841 aacgtcctcg aaaaaggcta tgacctcacg caataccgtg aaaagttagg gatggttttc
     3901 caatccttta acctctttga aaatctcaac gttcttgaaa acacaatcgt cgctcagaca
     3961 actgtcctta aacgcgaacg cacagaagct gaagaaattg ccaaagaaaa cctggaaaag
     4021 gtcggcatgg gagaacgcta ctggcaagcg agacccaaac aactctcagg tggtcaaaaa
     4081 caacgtgtgg ccatcgctcg tgccctttca atgaatcctg acgccattct ctttgatgaa
     4141 ccaacatcag ctctcgatcc agaaatggtt ggagaggtcc tcaaaatcat gcaagatctg
     4201 gctcaggaag gcttgaccat gattgtcgta acccatgaaa tggaatttgc ccgtgatgtc
     4261 tctcaccgtg ttatctttat ggataagggt gtgattgctg aagaaggtaa accagaagac
     4321 ctcttcacta atcctaaaga agaccgtaca aaagaatttc ttcaacgtta tctcatactc
     4381 aatgaaaatc aaagagcaaa ctaggcagct agccgcaggt tgctcaaaac actgttttga
     4441 ggttgcagat agagctgacg tggtttgaag agattttcga agagtatcaa ataaaaagaa
     4501 aaggctgcat caaccgtgca gtctttttgc tgtcctcaaa atgaaaaggc agattctcta
     4561 tctctactac ttgaaggtca agaatccacc gttatccaaa gattaatctt caaatttttc
     4621 atgatttttt tcatagaaat caattaagcc tagggctgtt tcaaatgaaa tattttttac
     4681 ttttgcacga ccctgcgcca gagcaatgat agacatttca cgcgcattcg tttctttaga
     4741 gatacggtag cctgtgattt tcttatcacg aacccagcca acaactgatt ctactttttc
     4801 aaagtttgac ttagccatgt ttttctccta ttttcttctt tataatattt aattgtatac
     4861 gtttttacta cttttgtcaa taaaaaaaac atatattcaa cgaaaataat tagttcagaa
     4921 ttttcttact tcctttttaa atccgataat atataggttt tgctagcctc tacaccataa
     4981 atatttgcta ataaaaatac tgtattatta ccctcttaag gtgcaaaatt attcaagttc
     5041 ttaaaaaatg cttctctatt tccgtaagat agcttatttc ttcgatttaa gaactgaaga
     5101 tgatagttct aacctttaga aatccaagcc ttctctagct caaaaactcc tatctttctt
     5161 cctcaataaa tcatctgaga gataggtatt ccttatatcc ttcaaattca tcactccttt
     5221 ggatattgag gtgctagcct agaagcagac acccgattat ctaaaccatc cttaatagag
     5281 atgacaagac agataagctc agttaattac ttatatatct ttttaatatg gtatatttgt
     5341 caacaaaaag aaatcaaatc ataccttata ctcaatgaaa atcaaagagc aaactaggaa
     5401 gctagccgca aattgctcaa aacactgttt tgaggtttca gatagagcta acgtggtttg
     5461 aagagatttt cgaagagtat taaagacagt atgactatgc ttgtcaatag ataggaaatc
     5521 aaaatgaagg atggaaatct gagccaaaca tcataagtgc tataatgaac agaagtaaga
     5581 attgcacgat aagttttcta aaaccatagg aatcgtcccc tttcttcatc ttcattatag
     5641 cacatataca tcagttgtac aaataatatg atattttttt attagtcctc agacaagcat
     5701 attataaagc gtttcattac catttaagtt aatataagct ggatcaaaac cttccattct
     5761 acggatcaaa cctgcataat catgcttatc tgccaaagcg atcccaatca atactgggcc
     5821 tgttcccttg ctagctcgtt tgatatactc aaaacgtgtg atatcatcat ttggccccag
     5881 gatatcattt acaaactcac gcaaagctcc tggacgctgt gggaaattga ccacaaagta
     5941 atgtttgata ccatcataaa tcaaggcacg ctcttccatt tctggcatac ggttgatatc
     6001 attatttcct ccagaaatga tacaacaaat ggttttcccc ttaatatatt cagctaaaac
     6061 ctctaaagag gcgatactag ccgctccagc aggttctgcg actatccctt gcttagagta
     6121 aaggtcaatc aaggtttcag aaatcaatcc ctcatcgaca cctaccaaag tttgaatatg
     6181 ttgacgagtt gcttcatagg tcaactgacc taccttttgc acagcaatcc catccgcaaa
     6241 tttatcaatt tccttgagtt tgactggacc tccagcctca aaggcagctt tcatggaacg
     6301 cgctccattc gcctctactc cgataacctc aatctctgga cttgtttcct tgatataggt
     6361 agaaaccccg gcaatgagac cgccaccacc aacaggaacc aagacagcat caaaatcaat
     6421 cgattctttt cgagcttctt ctaaaatctc ataagcaact gttccttgac ctgcttgaac
     6481 atgagcatca tcaaaaggat caataaaggt acgattttca gagactgtaa attcttgagc
     6541 tgctttggct gaggcatcaa aggtatctcc aactagttta atagttacaa aatccccacc
     6601 aaaaaagcga acctgaccaa tcttttgttg tggcgtagta ataggcataa agatagtagc
     6661 aggaattttc atttcattac aagtataggc tactccctgc gcatgatttc ccgcagaagc
     6721 gcagactacc ccacgttcac gttcttcctt gctgagctgg gaaatggcat aataggcacc
     6781 acgaatttta aaggagcgaa cacgctgggc attttctttt ttcaaataaa tcttagcacc
     6841 atacttctcc gataaataat gatcgtaatc cagtggagta ttcacaacca caccgttcaa
     6901 gaccttgtga gccttgatta tatcttttga acttaacata taaaacctct ttttctataa
     6961 aaaacagtca ctaatgactg ttttttagtt attcatctcc gaaaacttca gtaacacttt
     7021 catggtattt atgagccata cgaatcgaaa actctttagg aagttcataa cgatttgtgg
     7081 aaatccaatc atataaccat tctaccaaac tttcttcact atcattacga tggaagataa
     7141 aatagtttaa aatttcttta tattctacat cattcaaatc caacgtgtct acaaattgac
     7201 tacgaggaac aaaattagaa tctaattttc ttagttgatc tgtcaattct cccaattgta
     7261 aatccaactg aaaaaatagt ttttgcagtt gcctatcagt ttcatcacat cctgtcttga
     7321 tagaaaacaa attggtaata ttcatatgat ttcaacccct aaataagtac atctaatatc
     7381 ccacgaatag ctgaagctaa agttttcgca gtccagtcat ttaaacccga ttctttcaat
     7441 ccagatgcaa taatttgctc tatttgatat aaaccgccat ctatcgtctc atttattctg
     7501 cgaagaggac cttcaatata ggataaagct tctccgaatc taacagctga tttaggtcct
     7561 aatgatttac caactatatt tgttaatgtt ttttatttct caaaatccat ttcgctatat
     7621 aacgagcaga tttagtcgca ccagctctct caaacggaga atgaatttca agaccttctt
     7681 ctctagctat ttgtgccaat ttgaagaatt gttcaaaggt catatgcaac ctaccatctt
     7741 caaaattagc atcatcataa atgttaatta cagcattttc atcatacgac caatctgctt
     7801 tattttggtc tgctagcact attaccgacg gtgtaattac attatacaac tagaaccaat
     7861 aataacgatg caatttttct cataatcttt ttcataaaat catctccagc gctatatatt
     7921 tctaattagt tatagatttt gaatgcatca tcgtcgtttt taccaacaaa tggcattgct
     7981 ttacgcaatt ctgcaccaac tttttcaatt tcaaggttag ctgcttgttc acggtaagca
     8041 gtcaattttg gacgtccagc tttatagtca tttacaaagt catttgcaaa tttaccattt
     8101 tggatgtctg ccaagacagc cttcatattt tctttaactt gttcagtgat tacacgtgga
     8161 cctgatacat agtcaccgta ttcagcagtg tttgaaatag attgacgcat tttcttgaat
     8221 ccaccttcgt agatcaagtc aacgatcaat ttcatttcgt gaagaacttc aaagtaagcc
     8281 aattctggag cgtaacctgc ttctgtcaag acttcgaaac ctgcttcgat aagggcagtc
     8341 aaaccaccac aaagtacagc ttgttcacca aacaaatctt cttcagtttc ttctttgtaa
     8401 gttgtttcaa gaagacctac acgagccgct ccaacacctt tacaccagtc catagcaatg
     8461 tttttagcat ttcctgttgc atcttggtat actgcataaa gagctggaac accaaatcct
     8521 tcttcgtaag tacgacgtac caagtgtccc ggtcctttag gagcacacat gaagacatct
     8581 acatccgcag gaactttgat aaattcaaag tggatgttga aaccatgggc aaatccaact
     8641 gcgtttccag cttccaagtt tggagcgatt tctgcttcgt acaattcttg ttgaatttcg
     8701 tctggcgcca agatcatgat aacatcagcc aacttagtag cttctactac tgtgtaagta
     8761 tcaaatccat cttcttttgc tttatcaaaa gatttacctg gacgtacacc gataataacg
     8821 tcacgacctg aatcacgcaa gttttgagca tgcgcatgtc cttgtgaacc ataaccgata
     8881 acggcgattt ttttaccgtc aagtgctgct actttaacat ctttttcata ttccatttga
     8941 actgtcatag cttttctctc ttttctatta tttattgcct tttaggctgg tttaataaat
     9001 ttaagttgga tttttaatcg cgggtaaatc cagttgcacc cgttcgagca atattgcgaa
     9061 taccgtatgg gcgaatgact cgcaataggg cttcgctctt ttctgcattt cccgtcatct
     9121 gaatggtaat cgagcttggc gctacgtcta ccactgttgc acggaaaggt tgaataatcg
     9181 ctaaaatctc agctctcttc tcagctggcg ctgacatctt aaccaaaatc acctcgcgct
     9241 ccaaatgagg cttgtctgta atatctcgaa tgcgaatcac atcaatctga cgattgagct
     9301 gtttgatgat ttgctccact tcatcatgag aagcaacatc aataataata gtgatacgcg
     9361 atacattcgg atcttctgtt gctccaacag agatgctttc aatattaacc tgacgacgag
     9421 ataggacacc tgtaaagcga ttgaggactc ctgaacgatt ttgtagtttt gctgttaaca
     9481 ttctacgcat ggaactgcac ccccaacatc tcatgattac tcttaccagc cggtaccatt
     9541 ggtaacacct gttccttacg agaaatatct acctcaatta gcataggaac atcctcagtg
     9601 atgacttcaa ggtcttgagc caaggtctca ggattgtcaa acttatagtt tttaatacca
     9661 taagcctgcg ccatcaattg gaaatcagga agggtatcaa agaccgactc tgatgttctg
     9721 ccttcataga aggattcctg ccactggcga accattccaa gtgaatgatt gttcagcata
     9781 accaccttga ttggcacctt gtaaatattc aaaatagcca actcctggtt ggtcatttgg
     9841 aaaccaccat ccccaacaaa caagactact tccttatctg ggttagcaat tttagcaccg
     9901 attgctgctg gaattccaaa gcccattgtt cccaaaccac ctgaagtcac taactgacgc
     9961 tcattttggt agggataata ctgagctgtc cacatttggt gttgaccaac gtctgttacc
    10021 acaatggcat ctccattcgt caattcacca attcgttcaa taactgcttg cggttgaacc
    10081 acacgctctt tcttgtcata agaacgaaca cgattcttgt ctttagtgac tttctcaatc
    10141 cacttttcag tattgttgtg aactgttggt tctgctagca acatttgcaa ggccttctta
    10201 gcatctccaa ctacaggaat gtctgcactg ataatcttgc caatctcagc tgggtcaata
    10261 tcaatgtggg caaccttagc attcttagcg aaagtcttag gattccccgt caaacggtca
    10321 tcgaaacgag aaccaatact aatcataaag tccgcttccg tcatggcaat atttgctgcg
    10381 aatgacccgt gcatgcctcc cattccaaga aagagtgggt gactcgttgc aatcgttcct
    10441 tgtcccaaaa gactggttac cactggaatt tgatagcgtt ctgcaaattc atttagttcc
    10501 gtagcagcct cagcataact aattccacca ccagctaaca agactggctt tttagccttg
    10561 gacaattgct tcaagatttt cttgatttgc atatcattcg gctcaagagt cggctgataa
    10621 cttggtaaat tcacttctgg tgaataaatg aagtctgttt ctaaagcaga tatgtctttt
    10681 ggtaggtcaa ttacaactgg ccctggacgg cctgtagttg cgatatggac agcttccgta
    10741 atgatacgcg gaatatcagc tgtctcacga acttggtaat tgtacttagt gattggcatg
    10801 gtaattccta cgatgtctgc ctcctgaaag gcatccttcc caatccctgc tcgcgccacc
    10861 tgacctgtaa agaccaaaag gggaacgcta tcgctcatag catccgcaat ccctgtaatg
    10921 gcatttgttg ctcctggtcc actagtgacg acggcaacac ccaactttcc agttgatttg
    10981 gcataacctt cagcttcatg caaacaacct tgctcatggc gccctagaat gtggcgaatg
    11041 cctttaaaat tatatatcgc atcataaaaa ggcaaaaccg caccaccagg ataaccaaag
    11101 atggtatcaa ctcctaaatc acgaagtgtt tccaaaacta ggtccgaccc cgtcttagga
    11161 gattctaaac tgattttctc cattgttccc ctttctttcc tcttaaaaat aacttgttac
    11221 tatcatacca ttttttcaaa atttttcaag ataaaagaag aaattttctg aattttctat
    11281 tttaacgttt atttatgaat gttatttttg tttttattaa aaagataccg atttcattta
    11341 ctaaaaaaga aaaaagaact gatttctcag tccttcatta atcttattcc acactaaata
    11401 ggtatgggta aacaggttgt tgaccttggt gaatctcgac ttcaacgtct tcgaattctt
    11461 ctacgatttc ttgagcgatt tcattggcaa gttcttcgct tccgtcttca ccgacataga
    11521 aggttacgat ttcactatct tcatccaaca tatgtttcaa ggtttcagtc aatgtttggt
    11581 gcatatcagg gtttgacaca agaatttttc catccaccat acctagatta tcgttttcat
    11641 ggatttctaa gccatcgatc gttgtatcac gcacggctgt tgtgacgctt ccgctaacga
    11701 catcgctaag agcagctgtc atacgctctt ggttttcttc aatggacttg cttggatcaa
    11761 aggcaagaag acttgtcata ccttgaggaa gagtgcgagc ctctaccact actgctggtt
    11821 gctccaaaac ttctgccgca gattgagctg ccatgaagat gttcttgttg tttggcaaga
    11881 agatgatgtt acgggcattg acctgttcaa cagccttgat aaagtcttct gttgaagggt
    11941 tcatggtttg accgccttcg ataacataat ccacgccttg agaacagaag atatctgcta
    12001 gacctttacc agccaccaca gcaatcaaag catactcttt ttcttcagcc gacttgataa
    12061 cttgagtagc ttctttctca acctgtgctt cgtgttggtt acgcatattg tcaactttta
    12121 ccttgaccaa gctaccatat ttgagacctt cttgcataac aagtcctgga tcttctgtat
    12181 gaacatggac tttgacaatt tcatcatcgt taacaacgag gagagaatcc ccaagctcat
    12241 ccaagtagtt acggaattca tcgtagtcaa aatctttagc ataggttgga ccttgcttaa
    12301 gagctaccat gatttcagta cagtaaccaa acgtgatgtc ctcagtcgct acgtgaccag
    12361 ctacagactt atgatgctct acattgatca tctcactcat gttggcagga gtcgctacaa
    12421 agtcctcaga tgcaatatat tcgccagtaa gggctgaaag gaaaccttcg tagatgaaga
    12481 ccaatccttg accacctgag tccacaacgc caacttcttt caatactgga agcatgtctg
    12541 gtgttttagc tagagctgtt ttagcacctt ccaaggctgc gcgcatgact tcaacagtgt
    12601 catctgtttg ctcagctttt ttcttagcac cgatagcagc tccacgagaa actgtcaaaa
    12661 tcgttccttc aacaggtttc atcactgcct tataggcaac ttccacacct gattggaagg
    12721 ccagagccaa gtcttgacct gttaactcgt ctttatcctt gatagcttgg gaaaatccac
    12781 ggaaaagctg agacgtaatc actcctgagt tcccacgcgc acccatcaaa agccctttgg
    12841 caagaatgct cgctacttct ccaactgtag aagctggctt gtctgcaact tctttagcac
    12901 cattttcaat ggtcattccc atatttgtcc cagtatctcc atctggaact ggaaagacgt
    12961 ttaatgaatt gacatattca gcttgcttat tcaagcgagt tgatgcagcc tgcaccattt
    13021 cttgaaataa gctagtagta atttttgaca cggttattct cctacaactt tgatattttg
    13081 aatgtagaca tttacagtct gagcagtaat tccaagctgg ttttccaagc taaaggcaac
    13141 acgctcttga atgttttttg acacttcact aatctttgtt ccgtagctta acacggtata
    13201 tacatcaact gcaatactgc catcttcggc tgcctttacg acgacacctt tagaataatt
    13261 ttccttacct agcagggctt ggaaattatc tttgagggca tttttactag ccataccgac
    13321 cacaccaaaa atctcagttg ctgctccacc tacgacggtt gcaatcactt catctgttag
    13381 ttcgatttga ccatcttttg tattaatttt tacagtcatc ctttttacct caactagttg
    13441 atactctatt ttatcatatt tcagcccaag tgtaaaagca gaatgatgta tcagcggata
    13501 tttactctat ttttcaaacg atgttatacc cacaataaaa gaaaaaagac cctaaggtct
    13561 cctttgcttt tattattaaa cgcgttcaac tttacctgat ttcaaagcac gagctgaagc
    13621 ccaaactttt ttaggtttac catcgataag aacagtaact ttttgaaggt ttggttttac
    13681 ggcacgtttt gtttggttca tcgcgtgtga acggttgttt cctgatacag tcttacgacc
    13741 tgtaaagtaa catactttag ccattgtgtt ttcctcctat tagatctaat atagcggatg
    13801 tgctagcacc acataccgta ctatgttatc acactttctt gttttttgca agggaattgg
    13861 aagatttttt atttgtgtct taaatcaggt cttgcgtgac attttctgct ctccacatgc
    13921 catcgttgat taacagaaca ccagaattaa aattatgtgt ataaaaatca tctctaactg
    13981 cagctaaggg tatagccgtc aagtccaaat cccacagctc atctatcgat tttcttacaa
    14041 caatatctga atccaaatac agtacacgag actcgcttac atactttgga ataaaatacc
    14101 taaaaaagcc gcatatgaaa gtccctcaaa ggggagacga taacctttca gaatattact
    14161 gtcaatctaa acattcacaa tctcactatt caaagtctct agtctttttt ccatcaattg
    14221 gaaccattct cgcggaaggt catcattaaa aacataaaac ttaagattat aatgatgaac
    14281 acaaagagat tttattgttg tttcaacttt atccatataa gcattatctg cacctaagac
    14341 aatcgctttt ttctcttttt tctcttcttt cactttttat ctcatttctt tttattccca
    14401 tcatatgttt ctacgtaacc attattttcg cctattcgtt cgtaaaacca taccagtgga
    14461 gattttagat gaagtcccat tacggtttac aatttttaca ttacgacacg gagttttaca
    14521 aatcgatttc atttgccaaa cgtagttagt gaggcagtta gctagttcgc caaatagcga
    14581 ctagcgtcca acaatttgga actttagttc caattgttgg tactgagtca catcttctcc
    14641 tctaactcta cgtctggata cttgtcagca aaccagcgga gggcaaagtc attttcaaag
    14701 agaaagactg gttggtcaaa acggtctttg gctaagatat tgcgacttga cgacatccgt
    14761 tcatccaagt cctcaggctt gatccaacga acggtctttt tacccattgg gttcataact
    14821 acttctgcat tgtactcgcc ttccatgcgg tgtttaaaga cttcaaactg gagttgacct
    14881 acagcgccta gcatgtactc acctgtttgg taattcttat aaagctgaac ggctccttct
    14941 tgcaccaatt gctcaatccc cttgtggaag gatttttgct tcataacatt cttagcagaa
    15001 actttcatga aaatttcagg agtaaaggtt ggcagtggtt caaattcaaa cttgttttta
    15061 ccaactgtca aggtatcccc aacttgataa gtaccggtat cgtaaacccc gataatatca
    15121 cctgccacgg cattgatcac attctcacga ctttccgcca taaactgggt aacattagat
    15181 agtttggccc ccttaccagt acgagggaga ttgacactca tgccacgctc aaattcgcct
    15241 gatacgatac ggacaaaggc aatacggtca cggtgacgag gatccatgtt ggcttggatt
    15301 ttaaagacaa agcctgagaa atccttgtca taaggatcca caatttcacc gtctgttttc
    15361 ttgtgaccat gtggttctgg agcaaacttg aggaagattt caaggaaggt ctgcacacca
    15421 aagtttgtca gggctgaacc gaaaaagaca ggcgtcaatt ctccagccag aatagcttcc
    15481 tctgaaaact cattcccagc ttcatttaaa agctcaatgt catccttgac ttgctcgtag
    15541 aaaggattgc taccaaagag tttgtccccg tcttctagac tggcaaaacg ctcatcccct
    15601 ttgtaaagct ctaaacgttg gttatagagg tcatacaagc cctcaaaggc tttccccatc
    15661 ccgataggcc agttcatagg gtagctagca atgcccaaga tttcttccaa ttcttgcaag
    15721 agatccaaag gctcacgacc gtcacggtcc agcttgttca taaaggtaaa aactggaatg
    15781 ccacgatgtt tcacaacctc aaacaatttc ttggtttgag cctcgatccc cttggcagag
    15841 tccacgacca tgaccgcagc atccaccgcc atcaaggtac gataggtatc ttctgagaag
    15901 tcctcgtgcc ctggcgtgtc taagatattc acgcgcttgc cgtcgtagtc aaattgcata
    15961 acagatgaag taacagaaat cccacgttgc ttctcgatat ccatccagtc agatttagca
    16021 aaagtccctg ttttcttccc ttttaccgta ccagcctcac gaatctcacc cccaaagtag
    16081 agtaactgct cagtgatggt tgttttcccc gcgtccgggt gggagataat ggcaaaggta
    16141 cgacgtttct taatttcttc ttgaatattc ataagttctc tttctttgat tctctatttt
    16201 tcttgtttca atagctgaga atgattttta cattggattt taccattcct ttcaacattc
    16261 cattatatcg gattttagca tttttttcaa tttctatttc ttttcacttc cccctccctt
    16321 atttatagga aaatatggta aaatagaaca gactaaaaat catcatttca cgaaaggatg
    16381 caagatgaaa attacgcaag aagaggtaac acacgttgcc aatctttcaa aattaagatt
    16441 ctctgaagaa gaaactgctg cctttgcgac caccttgtct aagattgttg acatggttga
    16501 attgctgggc gaagttgaca caactggtgt cgcacctact acgactatgg ctgaccgcaa
    16561 gactgtactc cgccctgatg tggccgaaga aggaacagac cgtgatcgct tgtttaaaaa
    16621 cgtacctgaa caagacaact actatatcaa ggtgccagct atcctagacg atggaggaga
    16681 tgcctaatga cttttaacaa taaaactatt gaagagttgc acaatctcct tgtctctaag
    16741 gaaatttctg caacagaatt gacccaagca acacttgaaa atatcaagtc tcgtgaggaa
    16801 gccctcaatt catttgtcac catcgctgag gagcaagctc ttgttcaagc taaagccatt
    16861 gatgaagctg gaattgatgc taacaatgtc ctttcaggaa ttccacttgc tgttaaggat
    16921 aacatctcta cagacggtat tctcacaact gctgcctcaa aaatgctcta caactatgag
    16981 ccaatctttg atgcgacagc tgttgccaat gcaaaaacca aaggcatgat tgtcgttgga
    17041 aagaccaaca tggacgaatt tgctatgggt ggttccggtg aaacttcaca ctacggagca
    17101 actaaaaacg cttgggacca cagcaaggtt cctggtgggt catcaagtgg ttctgccgca
    17161 gctgtagcct caggacaagt tcgcttgtca cttggttctg atactggtgg ttccatccgc
    17221 caacctgctg ccttcaacgg aatcgttggt ctcaaaccaa cctacggaac agtttcacgt
    17281 ttcggtctca ttgcctttgg tagctcatta gaccagattg gaccttttgc tcctactgtt
    17341 aaggaaaatg ccctcttgct caacgctatt gccagcgaag atactaaaga ctctacttct
    17401 gctcctgtcc gcatcgccga ctttacttca aaaatcggcc aagacattaa gggcatgaaa
    17461 attgctttgc ctaaggaata cctaggcgaa ggaattgatc cagaggttaa ggaaacaatc
    17521 ttaaacgcgg ccaaacactt tgaaaaattg ggtgctatcg tcgaagaagt cagccttcct
    17581 cactctaaat acggtgttgc cgtttattac atcatcgctt catcagaagc ttcatcaaac
    17641 ttgcaacgtt ttgacggtat ccgttacggc taccgcgcag aagatgcgac taatcttgat
    17701 gaaatctatg taaacagccg tagccaaggt tttggtgaag aagtgaagcg ccgtatcatg
    17761 ctgggtactt tcagtctttc atcaggttac tacgatgcct actacaagaa ggctggacag
    17821 gtccgtacac ttatcattca agatttcgaa aaagtcttcg cggattacga tttgattttg
    17881 ggtccaactg ctccaagtgt tgcctatgac ttggattctc tcaaccatga cccagttgcc
    17941 atgtacttag ccgacctctt gaccatacct gtaaacttgg caggactgcc tggaatttcg
    18001 attcctgctg gattctctca aggtctacct gtcggactcc aattgattgg tcccaagtac
    18061 tctgaggaaa ccatttacca agctgctgct gcttttgaag caacaacaga ctaccacaaa
    18121 caacaacccg tgatttttgg aggtgacaac taatgaactt tgaaacagtc atcggacttg
    18181 aagtccacgt agagctcaac accaattcaa aaatcttctc acctacttct gcccactttg
    18241 gaaatgacca aaatgccaac actaacgtga ttgactggtc tttcccagga gttctaccag
    18301 ttctcaataa aggggttgtt gatgccggta tcaaggctgc tcttgccctc aacatggacc
    18361 tccacaaaaa aatgcacttt gaccgcaaga actacttcta ccctgataat cccaaagcct
    18421 accaaatttc tcagtttgat gagccaatcg gttataacgg ctggattgaa gtcgagctag
    18481 aagacggtac gaccaagaaa attggtatcg aacgcgccca cttagaggaa gacgctggta
    18541 aaaacaccca cggtacagac ggctactctt acgttgacct taaccgccaa ggggtacctt
    18601 tgattgagat tgtatctgaa gccgatatgc gttcgccaga ggaggcctat gcttatctga
    18661 cagccctcaa ggaagttatc cagtacgctg gcatttctga cgttaagatg gaagaaggtt
    18721 ctatgcgtgt ggatgccaac atctcccttc gtccttatgg tcaagagaaa ttcggtacca
    18781 agactgaatt gaagaacctc aactccttct caaacgttcg taaaggtctt gaatacgaag
    18841 tccaacgcca ggctgaaatt cttcgctcag gtggtcaaat ccgccaagaa acacgccgtt
    18901 acgatgaagc gaataaagca accatcctca tgcgtgtcaa ggaaggggct gctgactacc
    18961 gctacttccc agaaccagac ctacccctct ttgaaatttc tgacgagtgg attgaggaaa
    19021 tgcggactga gttgccagag tttccaaaag aacgtcgtgc gcgttatgta tctgaccttg
    19081 gtttatcaga ctacgatgct agtcagttga ctgctaataa agtcacttct gacttctttg
    19141 aaaaagctgt tgccctaggt ggtgatgcca aacaagtctc taactggctc caaggggaag
    19201 tcgctcagtt cttgaatgct gaaggtaaaa cactggaaca aatcgaattg acaccagaaa
    19261 acttggttga aatgattgcc atcatcgaag acggtactat ttcatctaag attgccaaga
    19321 aagtctttgt ccatctagct aaaaatggcg gtggcgcgcg tgaatacgtg gaaaaagcag
    19381 gtatggttca aatttcagat ccagctatct tgatcccaat catccaccaa gtctttgccg
    19441 ataacgaagc tgctgttgcc gacttcaagt caggcaaacg taacgccgac aaagccttta
    19501 caggattcct tatgaaggca accaaaggcc aagccaaccc acaagttgcc cttaaactac
    19561 ttgcacagga attggcgaag ttgaaagaaa actagacaga acaaaaccag ccctaaggtt
    19621 ggttttttct tctctaccaa ctcccaataa ctattttggc tttatttcca gagtatttta
    19681 tggtaaaatg aagagtaata atatttatta aagaggtaaa aacatgattg aagcaagtaa
    19741 attaaaagct ggtatgacct ttgaaacagc tgacggcaaa ttgattcgcg ttttggaagc
    19801 tagtcaccac aagccaggta aaggaaacac gatcatgcgt atgaaattgc gtgatgtccg
    19861 tactggttct acatttgaca caagctaccg tccagaggaa aaatttgaac aagctattat
    19921 cgagactgtc ccagctcaat acttgtacaa aatggatgac acagcatact tcatgaatac
    19981 agaaacttac gaccagtacg aaatccctgt agtcaatgtt gaaaacgaat tgctttacat
    20041 ccttgaaaac tctgatgtga aaatccaatt ctacggaact gaagtgatcg gtgtcaccgt
    20101 tcctactact gttgagttga cagttgctga aactcaacca tctatcaaag gtgctactgt
    20161 tacaggttct ggtaaaccag caacgatgga aactggactt gtcgtaaacg ttccagactt
    20221 catcgaagca ggacaaaaac tcgttatcaa cactgcagaa ggaacttacg tttctcgtgc
    20281 ctaatctcta gaaagaggtc attctatggg aattgaagaa caacttggcg aaatcgttat
    20341 cgccccacgt gtacttgaaa aaatcattgc tatcgctact gcaaaggtag agggtgttca
    20401 ctctttttca aacagatcag tgtctgatac cctttcaaaa ctttcactcg gccgtggcgt
    20461 ttatcttaaa aacgtggacg aagaactcac agcagatatc tatctctacc ttgagtacgg
    20521 agtaaaagtt cctaaggtag cggttgctat ccagaaagct gtcaaagatg ccgtccgtaa
    20581 tatggctgat gtagaactcg ctgctatcaa tattcacgtt gcaggtatcg tcccagataa
    20641 aacaccaaaa ccagaattga aagatctatt tgacgaggac ttcctcaatg actagtccac
    20701 tattagaatc tagacgccaa ctccgtaaat gcgcttttca agctctcatg agccttgagt
    20761 tcggtacgaa tgtcgaaact gcttgtcgtt tcgcctatac tcatgatcgt gaagatacgg
    20821 atgtacaact tccagccttt ttgatagacc tcgtttctgg tgttcaagct aaaaaggaag
    20881 aactagataa gcaaatcact cagcatttaa aagtaggttg gaccattgaa cgcttaacgc
    20941 tcgtggagag aaacctcctt cgcttgggag tttttgaaat cacttcattt gacactcctc
    21001 agctggttgc tgttaatgaa gctatcgagc ttgcaaagga cttctccgat caaaaatctg
    21061 cccgttttat caatggactg ctcagccagt ttgtaacaga agaacaataa aaagaaaaag
    21121 tgtttgatag atatcaaaca ctttttcttt gcctcccact atctaaaaaa atgataatag
    21181 atataattgt aaacaaaaat ccagataggt tttgcatgat tgagaaagtt aaaaaaacta
    21241 tggcagagaa tcgttaatct cagattgtcg gtagaacgat aaacaagggc aaaaaagaaa
    21301 ccaatcagac tataatataa taaactaatt ggatctctgt gagatagtat caaatggcta
    21361 atcccaaaga taaggcctga taagatgaca tctaaatagt actttgactt aggaaagaag
    21421 gtattcataa aataccctct atcaagagtc tcctcaaaaa caggaccgat gattacaagc
    21481 aggacaaaag ataagatagt cgataaaaag gttggttgtc catttgaaag aaccacggta
    21541 aaatactcat catgaatatt cctatggtta atcaaatgag catagcgtgc ctaaaaatta
    21601 ccgagaatct gataaaccac ataagttgca aataaggaga agacaaatga ccagtcccag
    21661 ctctttttct tttttaacct ccaaattaat agaaaaggaa acttcccact aatcccactg
    21721 ttaaaataag agaatagaca tcagctccta accctaaaat gatcgtcaca tacaatccaa
    21781 ttgtttgtgg taaataggta gatagtaaaa taataagcaa aaatattcca aattgtctta
    21841 gtttctttgt gtttctcatc gtactttttt gaaagattac cctgttcgga agccgtactt
    21901 ccaagcatct atataagaat taagtgcccc ttgcctcata tagggagcaa attctctata
    21961 atataaccat ctactatatc catcttccca aacagcaaga ccacctgaag tttgctccaa
    22021 gtcctcagtt gaaagaactg taaatgtttc tgtacctgtc attgcaagta ccttcttaaa
    22081 atagattgtt gtaggctcac atttatagta tatttctttt tttgtctatt ttatagtcta
    22141 tctcctcaac tggcaatttt tcgacctgaa ttgcattttt ccataaaaaa tgagaccttt
    22201 ctagtctcat ttagtcattc ttagtatttt ctaaatcgtt gatagcgttc ttccagcaac
    22261 tcttctagcg gtttttgtga aagtctagcc agctccgttt ggagttcttt tttgacactc
    22321 ttaattagtt ctttactaga aagtcctact tcagaaatca ccttatccac cacgtccatt
    22381 tctaacagtt catgcgaagt gattttcatc agttctgctg cttccatggc gcgagtaccg
    22441 tccttccata aaatggaagc aaagccttct ggactgagaa tggcatagat agaattttcc
    22501 agcatccaga cacggtccgc gacagctaga gccagagccc cgcctgaacc accttcaccg
    22561 ataataatgg cgataatagg aactttcagg tcactcattt ccatgagatt gcgagcgata
    22621 gcttcccctt gaccacgttc ttccgctccg acaccaggat aagcacctgc tgtattgata
    22681 aaggtcacaa ctggacggcc aaatttctca gcctgtttca tcaaccgcag tgcctttcgg
    22741 tagccttctg gatgtggttg gccaaaattc cgtttgaggt tgtcttgcaa actcttgcct
    22801 ttttggatac caaccactgt tacagcttgg tctccaagcc aaccaatacc accaacaact
    22861 gcaccatcat cacgaaaaga acggtcacca tgtaattgga taaattcatc aaaaatgcct
    22921 gtcgcaaagt ccaaggttgt caagcgactc tgctcacgcg cttctctgac tatttttgca
    22981 atattcatct aggactccct ccatgcaatc tgactaggct agcaatcgta tctggtaagt
    23041 ctcttctttt gacaatagca tccacaaagc catgttctaa taggaattct gccttttgga
    23101 aatcctcagg caagctttca cgaaccgtat tttcaatcac acgacgccca gcaaaaccaa
    23161 ccaagctctg tggttcagcc agaatgatat cgccttccat agcgaaagaa gctgtcacac
    23221 caccagtcgt tggatctgtc aaaatggtca ggtaaaagag accagcattt gaatggcgtt
    23281 taaccgctgc agagatctta gccatctgca tgagactcat gattccttcc tgcatacggg
    23341 ctccaccaga ggctgtgaat aggacaactg gtaatttttc gacagtcgca tactcaaaca
    23401 aacgagtgat tttttcacct acaaccgtac ccatagaagc catgataaaa ttagaatcca
    23461 taatcccaag agccacagtc tgacctttaa taagagcagt tcctgtcaca acggcttcat
    23521 gcagacctgt tttttcacgc atagatgcca gtttcttttg gtaaccaggg aaatgcaagg
    23581 gatccttgct ttcaatccct gtaaacaatt ctttgaaggt tcccatatca atcgtcaaag
    23641 ccaagcgttc ttgggcagaa atacgaaagg tatagctaca gtgcggacag atacgttcac
    23701 ttcccagatc cttctgatag atggtatgct tacagcctgg acactgggaa aataattcat
    23761 ctggaacctc tggcttagct tgaggttttt ccctaaccga acgattggga ttgattcgaa
    23821 tatacttatc ttttttacta aatagagcca ttgattcccc ttttcggttt aaactcttaa
    23881 agtcatttta ttctttttct tgatatttag gtaagaaggt ttccatcaag aaggaagtat
    23941 cataatcccc agcaatgaca ttgcgatctg aaatgagatc aagctggaaa tctgcattgg
    24001 tctgcactcc ttcaatctct aattcataga gggcacgttg cattttcatc aaggcgtcaa
    24061 aacgattttc gccgtgtact atgattttgg caatcatact atcataataa ggcggaatgg
    24121 tataacctgg ataaactgct gaatccacgc gcaagccaac tccaccactt ggcagataga
    24181 gattagtaat cttacctgga cttggagcaa agttaaaggc tgggttttct gcattgatac
    24241 gacactcgat ggcatgaccg cgtaggacaa tatcttcttg cttaacagac aaaggctgac
    24301 ctgccgcaat gcaaatctgt tccttaacga tatcaacacc tgaaacaaac tctgttactg
    24361 gatgttctac ctgaacacga gtattcatct ccatgaaata gaaattgcta cttgcttcat
    24421 caagaagaaa ttcaatggtt cctgcattct catagccaac aaactctgcc gctcgaacag
    24481 cagcagcacc tatttcatga cgcagcgttt ttccgattgc aatcgaggga ctttcttcca
    24541 aaaccttttg gttattcctt tgaagagaac aatcccgttc acccaagtga atcacatgtc
    24601 catgctcatc acctaggatt tgaacctcaa tgtgccgagc tggatagata acccgttcta
    24661 tgtacatggc accattgcca taattggcct tggcctcact agaggcagtt tcaaaggcag
    24721 aaacgaggtc atctggtttt tcaaccttac gaatcccttt accacctcca cctgctgaag
    24781 ccttgagcat aacaggatag ccaatttttt cagcaacaat caaagcttct tcagagttat
    24841 gcacttctcc atctgaacct ggtataacag gcacacctgc tttaatcatc tgagcacgcg
    24901 cattgatctt atcccccatc atatccataa catgaccaga tggaccgata aacttgatac
    24961 ctacttcttc acacatggtc gcaaatttgg aattttcact gagaaatcca aaaccagggt
    25021 gaatagcttc tgcctcagtc aagactgcag ctgatagaac tgcattaata ttgagataag
    25081 actctgttgc cttgccagga ccaatacaaa ctgcttcatc tgccaacagc gtatgaagag
    25141 cttccttatc agcagttgaa taaaccgcta ccgtcgcaat ccccaattca cgtgccgcac
    25201 ggataatacg aaccgcaatt tcaccacgat tggcaattaa aatttttcga aacatggaga
    25261 acctccttag ttcccaattg caaaagtaag ggtaccactg gctgcaagct tgccatccac
    25321 ttcagccttt gcttcaacca cagctatggt gccacgacgt tttacaaaag tcgctgtcat
    25381 aaccaattgg tcgcctggta caacttgctt cttgaactta accttatcca taccagcgta
    25441 aaagaccagt tttcctttat tttcaggttt tgataactcc aacacaccag cagtttgcgc
    25501 caaggcttcc ataatcagaa cacctggcat aactgggtat tgaggaaagt ggccgttaaa
    25561 gaaaggctcg ttgatggtca catttttgat agcaacaatg gtatcctcgc tcacctccaa
    25621 gacacggtcc actagaagca taggataacg gtggggaagg gcttctttga ttccttgaat
    25681 atcgatcatt tgatacgtac caatccttta ccaaactcaa ccatttcttc gttagagacg
    25741 agaatttccg ttaccacacc atccttagga gctgggattt cattcatgac tttcatggct
    25801 tcgataatta ccaatgtttg accttttttg acactatcac caactgtaac gaaggcaggt
    25861 ttatctggtc cagcagccaa gtaaaccact ccaacaagtg gactctctac aagatttccc
    25921 tcactagcca cacttgcttc agctggagct ggaacttctt ctgctacagt ctctgctgga
    25981 gcagatgtag gagctactgg actcggtgtt gctagaacgg gtgctggagc gacttgagtt
    26041 gcaacttcag gcacaggtct cgcttcattc ttgctaaact gcaactcatc cgtcccattt
    26101 ttataagaaa attctctcaa acttgactgg tcaaattgag tcatcaagtc tttaatatcg
    26161 tttaaattca tacttatcta ttctcccaac gtttgaaagc aagaactgca ttgtggcctc
    26221 caaaaccaaa agtatttgaa atagcgtatg gaatttcttt ctccaagcct tgtccataaa
    26281 cgacattagc ttcgatataa tctgatactt cacttgtccc agctgtcatt ggtacaaagt
    26341 tatgacgcat agcttcgatg gtgacgatag cttctactgc acccgcagcc cccagcaaat
    26401 gtcctgtaaa agacttggtt gatgatacag gtacttcctt accaagaaca gctacgatag
    26461 caccactttc tcctttttca ttggcaggag ttgacgttcc gtgagcattg acataggcta
    26521 cttgctctgg agaaatctca gcttcttcca aggctagttt gatggccttg atagctccct
    26581 gaccttctgg atgtggagaa gtcatgtggt aggcatcaca agtatttccg taaccaacca
    26641 cttcagccag gatagtagct ccacgttttt cagcgtgttc aagactttct agaaccaaca
    26701 tccctgaacc ttcacccata acaaacccat tgcgatcctt atcaaatggg atcgaagcac
    26761 gagttggatc ctctgtagta gagagagctg ttaaggcttg gaaaccagcg atggcaaaag
    26821 gtgtgataga agcttctgtt cctcccacca acatcacatc ttggaaacca aacttaatgg
    26881 agcggaaggc atccccaatc gcatcatttg atgaagagca ggcagtattg atagatttac
    26941 aaacaccgtt tgcaccaaaa cgcatggcta cattcccaga agccatattt ggtaaagctt
    27001 ttggaagagt cattggtttg acacgtttgg gtcctttttc atgaaggcga agtacctgat
    27061 cttcaatttc cttgattcca ccaataccag atgcaacgat aacaccaaaa cgatccctat
    27121 taagagcctc tacatcaaga ttggcatgat ttacagcctc ttgggctgca tacaaggcat
    27181 ataaagaata gttatcaaaa cggttggtat ctttttttac aaagtattta tcgaacggaa
    27241 aatcttggat ttctgccgca ttatgcacat caaagtcact atgatcaaat tttgtaatgc
    27301 caccaatgcc gattttccca gttgctaaac tattccaaaa ttcttctggt gtatttccga
    27361 ttggagatgt tactccataa cctgttacca ctactcgatt tagtttcatt cttttcacct
    27421 ctagctttcg ctacatactt aagccaccat caatggcaat cacttgacca gttagataat
    27481 cttggcctgc taaaaatact gtcaaatctg caacctgctc tgcctgccca aattctttca
    27541 tcggaatctg agctagtgta gcttccttaa tcttatctga taagatagct gtcatatcag
    27601 actcaatcat tcctggagca atcacattga ctcgtatatt ccgactagcg acctcgcgtg
    27661 ccacagactt ggtaaagcca atcaagccag ccttagaagc agcatagtta gcttgaccaa
    27721 tattccccat caaaccaaca acactagaca tattaatgat agcaccttct ctggctttca
    27781 tcatcggttt caagactgat tgtgtcatat taaaggcacc agtcagattg accttgagca
    27841 ctttttcaaa atctgcttct gtcatcttga gcataagagt atcttgggta atccctgcat
    27901 tgttgaccaa aacatctact gaacccagtt ctgcaatagc ttgatcaatc atacgcttag
    27961 cgtctgcaaa atctgataca tctcctgaaa tgggaaccac cttgatacca tagtttgaaa
    28021 actcagcgag caattcttct gagattgccc cacgactgtt taagacaatg ttggctcctg
    28081 cttgagcaaa cttgtgggcg atggcaagac caattccacg actcgaacct gtaataaaga
    28141 tatttttatg ttctagtttc atttttttcc tttcaaaact tctacttatt ttagtctatt
    28201 tttctaaaag tgctactaaa ctcgcttgat cttccacatg agctaagtga gcagtttgat
    28261 caattttttt aacaaaacct gacaagactt tccccggtcc aatctcgata aagttgctta
    28321 tgcctgcttc ttgcatgacc ccaatacttt catagaaacg aacgggttcc ttgacctgac
    28381 gcgtcaagag ctgagcaatg tcctcttttt gcatcacagc agcttctgta ttgccgacta
    28441 ggggacaagt aaaatctgaa aaacttacct gagctagagt ttcagctagt ttctggctag
    28501 caggctcaag gagagcggtg tgaaagggac ctgacacctt aagaggaatc aagcgtttgg
    28561 cacctgcttc ttgcaaaagt tcaaccgctc gatcaactgc aaccacttct ccagcaatga
    28621 cgatttgtgc aggtgtgtta tagttggctg gagtaaccac tccaagttca gaagcttttt
    28681 gacaggcttc ttcaatgacc tctactggcg tattgagaac tgctaccatc ttgccagagt
    28741 cagcaggagc cgcttcttcc atataggctc cacgcttagc taccaaggca accgcatctt
    28801 caaaatccaa ggcgccgctt gccaccaagg cagagtattc tccaagagac aaaccagcaa
    28861 ccatatcagg ctgatagccc ttttcttgca ataaacggta gatagcaacc gaagtcgcta
    28921 gaatggctgg ttgcgtatag cgggtctgat tgagtttgtc ttcttccgta tcgatgagat
    28981 aacgtaaatc ataaccgagc acctgactcg ctcgatcaat cgtttcttta acaatcggat
    29041 actgatcata gaaatcccgt cccatcccta gatactgggc accttgacca gcaaataaaa
    29101 aggctgtttt agtcatttct tacaactcct gcccagcgag aggcttcttc ttgaattttc
    29161 ttagcggctc cgtaatacaa atcttttagg atttcttcag ctgtttcttc tttagaaaca
    29221 agccctgcga tttgacctgc catgacagag ccaccctcca catcaccgtg aacaactgct
    29281 ttggctaggg cacctgctcc catttgttca aagatttcta aatcaggatc ttcctgctta
    29341 aaggcatctt tttcagccag ttcaaaatct ctagtcaact gatttttaat agcacgaaca
    29401 gcatgaccaa agtgctgagc tgaaatcgta gtatcaatat cccttgcttt taaaattttc
    29461 tctttgtagt ttggatgggc attcgactct tttgcaacta caaaccgtgt ccccacctgt
    29521 acagcctctg cacctagcat aaagccagcc gcagcacctt caccatccgc aattcctcct
    29581 gcagcaataa caggaataga tatagctgtg gctacctgtc gcaccaaggt catggttgtt
    29641 aatttaccga tatgcccccc agcttccatt ccttctgcaa taacagcgtc tgcaccgatt
    29701 ttttccatgc gtttagctaa agcgacacta ggaacaacag gaataacgat tatcccagct
    29761 tcatggaaac gttccatata cttgcttgga tttcctgctc ctgttgtgac aactttaaca
    29821 ccttcttcaa taacgagatc cacgatatct tccacaaagg gagataagag catgatgttg
    29881 accccaaagg gtttatcagt caatgatttg attttatcaa tattggcctt gacaacttct
    29941 ttcggggcat ttcccccacc gataattcct aatcctccag ccttggaaac agcccctgcc
    30001 aaatcaccat cagcaaccca ggccatccct ccttggaaaa taggatagtc aatcttcaat
    30061 aattctgtaa tacgcgtttt catagtgcct ccaaccttcc ttgcttacgt aatagttcga
    30121 tttcaccata atttgacagt caaactatta cctaaacaag agggagtggg tttctcccta
    30181 ctccttctac taatattctg cttattttgc ttgctcttca acgtaagcaa ccaagtcacc
    30241 aactgttttc aagtcatttt ctgcttcgat ttggatatca aaagcatctt cgatttctga
    30301 gattacttgg aacaagtcca atgaatctgc gtccaaatca tcaaaagttg attcaagtgt
    30361 tacttctgat gcgtcttttc caagttcttc aacgataatt tcttgtactt tttcaaatac
    30421 tgccatgata ggactccttt aaaataaata gtttttttat aacaatgtgt tcaccacatg
    30481 attacctaaa ttgtaagaat gagcgtgccc caggtcaagc ctccaccgaa gcctgataga
    30541 agaacagttt ggctaccatc taaagggatg agaccttgtt ctacacactc tgaaagtaaa
    30601 atcgggatac tggctgcact ggtattgcca tattccatca tattagctgg aagtttggct
    30661 cggtcaacac caatttttct agccatctta tccaaaatac ggttattggc ttgatgaagt
    30721 agcagataat ccaagtctgt cacctctata ggagattcat caatagtctg cttgatagac
    30781 ttggctacat ctcgaatggc aaaatcaaag actgtgcgtc catccatctt caaaaatgaa
    30841 tctgcacttt cttgatctga aaatggagaa tgcaaacctg aatgcccata agttaaacac
    30901 tcgctgcgac ttccatcgct attgagactc tcagctaaga aatgctcttg ctcgctagct
    30961 tctaacaaga caccaccagc accatctcca aacaacacag ctgttgatcg atccgaccaa
    31021 tcgactgcct tagagagggt ttcactacca atcaccaagc ctttttgaaa gcgaccagaa
    31081 gcgataaact tttcagcagt tgaaagagca aatacaaatc cactgcaagc cgcggttaag
    31141 tcaaaagcaa aggctttatt agcaccaata ttagcttgaa cacgagcagc tgtagagggc
    31201 atcatcgaat ctggagtaat ggtagctagg atgataaaat ccagttcttc tcctgttatt
    31261 ccagcttttg ccatcagttt cttagcaacc tctgtagcca aatcactggt agattctgtt
    31321 cttgaaatat gcctttgtcg tattcccgtt cgacttgaaa tccactcatc attggtatcc
    31381 ataatctgag ccaagtcgtg atttgtaacc acttgctctg gcacataatg agcaacctga
    31441 cttatttttg caaaagccat tatttcaaat cctccaaaaa ttggtaaaga ttagtcaaac
    31501 ctttacccat gacagcaatt tcttcctcgc tcatgccatc aataattttt tctaccatgg
    31561 ccttgtggaa gcgtttatgc agtctatgaa tcaagcgacc cttctttgtc aaatgcagat
    31621 gcaccacacg acgatcctgt tctgaccgaa ctcgctcaat gtagccctta cgctctaaat
    31681 tgttcaaact tgtcgtaaca gttccaagag ttaccatcaa ctcttttgac acttgacttg
    31741 gagtcacatc tggagcctta ccgatgacat caatcgtatg catttctttg atggagatat
    31801 ccttaaaacg actacctctc aagttcactt cctcaattac aaggacattg ttaaatatag
    31861 atgttaaata ttcattgatt cgttggtagt ccatttttca tatccctcct tcttcaaaaa
    31921 acttttacaa tcaaaacatt tgacagaaag aattatatca aaattcaaag aatgatgcaa
    31981 gtatttttta ttttcctgta aatttaggtc ttcttctctc cgaatgagct cgcactcctt
    32041 ctttgaaatc ctctgtttga gctaaggatt tctgtaggtt cagttctaaa gtagcgtaac
    32101 cctgccagtc tttaaattgg ctctcccaaa ccaacttctt aatggcagca taggaattac
    32161 ttgaagcacg tcttaatttt ttaagaagct gttctctcgt cttttcaagt ttttcagctt
    32221 cagaaacgcg gtaaaccaac ccccattcta gggctttttc tgccgttaaa gcctctcctg
    32281 tcatagctaa ttgagcagca cgcgtcacac cgatactgcg gctcaagaga tgaattcccc
    32341 ctgcatctgg agccaaacca acaccaacaa aggcttggat aaacttagcc ttatccgtcg
    32401 ccaaacagaa atctgccgca acagccatat tcgctgcggc acctgcaaca gcaccgtcaa
    32461 cttccattaa aacaggtttg gctatttgct tgattttata agaaatcgta ttgaccaact
    32521 ctgcgatttt tgtcaatgat ggaatatcat cctcatccac tgcccgcttc atctctacca
    32581 aatctccccc aactgagaag acctttccat tggcattgat taagataaaa tgcacagctg
    32641 gattttcttc tgccaaagtc agagcttcta aaatctcctc acacatggga atatgaaaac
    32701 catttgcgac ctcaggacgg ttcaaggtaa ggattgccaa gtcctcttca agctgataaa
    32761 taatgtgttc catcagggct cctttttatt ttataagcag tttatactct tcgaaaatct
    32821 ctttaaaccg cgtcagcttc catctgcaac ctcaaaacag tattttgagc tgacttcgtc
    32881 tagcttctta gtttgctctt tgattttcat tgagtgttaa attatttcac tgtcaaagta
    32941 tatctttttt aggagcagaa agcaagaaaa aatcgactga aagtgtctca gccgataaga
    33001 aaggctcgct ttaggctaga aagcatctta ttttcttcat tgaaaaaagc ctttctttct
    33061 gctataatcg tataaaatac ttactttagg agttcttatg aaagttgtta aatttggagg
    33121 tagttctctt gcctctgcta gtcaattaga aaaagtttta aacatcgtca aaagcgattc
    33181 agagcgtcgt tttgtagtcg tttctgcgcc tggtaaacgc aatgctgaag atactaaggt
    33241 tacggatgcc ctgattaaat actaccgcga ctatgttgcg ggtaacgata ttagcaagag
    33301 ccaaagctgg attatcgacc gctatgctgc tatggttagt gaattgggac taaaaccagc
    33361 tgtgctagaa aaaatttcta aaagcattca cgccttggcc actcttccta ttgaagaaaa
    33421 tgaatttctc tacgatactt tcctagcagc cggtgaaaat aacaatgcca aattgattgc
    33481 tgcctacttt aaccaaaatg gtatcgatgc acgctatatg caccctagag aagctgggat
    33541 tgtggtcaca agtgaacctg gtcacgctcg catcattcca tcaagttatg acaagattga
    33601 agaattgaca aacaccaatg aagtccttgt cattcctggt ttctttggtg tcactaagga
    33661 aaatcaaatc tgtactttct cacgtggagg ttctgatatt acaggttcta tcattgctgc
    33721 tggtgtcaaa gctgacctct atgaaaactt tacggacgtt gatggtatct ttgcagccca
    33781 ccctggtatt atccaccaac cacactcgat tcctgagttg acctaccgtg aaatgcgcga
    33841 gttggcctat gcaggcttct cagtccttca tgacgaggct cttcttcctg cctaccgtgg
    33901 aaaaattcct ctggttatca agaataccaa caaccctgac catccaggta ctcgtatcgt
    33961 tctaaaacac agtaatgatg aatttccagt tgtgggaatt gctggtgact caggctttgt
    34021 cagcattaac atgtcgaaat acctcatgaa ccgtgaggtt ggatttggcc gcaaggttct
    34081 gcaaatcctg gaagaactta acatcggttg ggaacatatg ccaacaggta tcgacgatct
    34141 ttctatcatt ctccgttctc gccaactaac tcccatcaag gaagaagaaa tcctgcgtca
    34201 gttggttcaa aaggctaaag tagatcatgc agaaatcgaa cacgaccttt ctatcattat
    34261 gattgttggt gaaaaaatga agagccatat cggagtgact gctactgcga cacgcgctct
    34321 atctgaaaac aaaatcaaca tccagatgat gtctcaaggt tctagtgaag tttctattat
    34381 gtttgttgtc aataaagacc aagagaaagc agcaattaaa gccctctaca atgccttttt
    34441 tggagaaagt aaggaagact aagcatggcc caatcactta acaaaacagt actcctcagt
    34501 acgacaggca cttcctacct ctctatcgct gggaaagttg ggaaattcct tgtcggagat
    34561 caggctttgg aattttaccc agatgtcaat gtcgaacaat ttatccagat tccttggagc
    34621 catatcaatc agattggagc caatgtcact ggacgcaaaa tcagccgcca cttcgaagtc
    34681 tttacagaca aaggaaaatt cctctttgct tcaaaagact caggtgccat tctcaaaatt
    34741 gcacgcgaaa aactaggcaa tgacaaggtc gtcaaacttc cgaccctgat tcagacaatt
    34801 agccaaaaat ttaaaaatct atttgcaaaa aagtagaaac tttagtataa tcatagcaac
    34861 ggataagtag gttatcttct tctttcagaa agtctgcggg tactgcgagc agataggggg
    34921 gataggtgaa attctaccgt tagtaacaat tacatacaca atcaagtgca cacctgtgaa
    34981 gttggatgga accgtggccc tgccactcca acgttttgtc aggcgtgctt ttttcataaa
    35041 ggagttctta tgttagatat caaacgtatt cgtacagatt ttgaagctgt cgcagaaaaa
    35101 ttagctacac gtggtgtaga tgctgctgtc ttgaatgaaa tgaaagaaat cgatgctaaa
    35161 cgtcgtaaca tcttggtcaa ggttgaaact ctcaaagcag aacgtaacac agtttctgct
    35221 gagattgccc aagctaagcg caacaaggaa aatacagatg acaagattgc tgccatgcaa
    35281 aatctatctg ctgaggttaa agccttggat gctgaattgg cagaaatcga tgctaaattg
    35341 acagaattta caacgactct tccaaatatc ccagctgaca gcgttcctgt tgggactgac
    35401 gaagacgaca atgtggaagt tcgccgttgg ggtactccac gcgagtttga cttcgaacct
    35461 aaagctcact gggatctcgg tgaagacctt ggtatccttg actgggaacg cggtggtaag
    35521 gtaacaggcg ctcgcttcct cttctataaa ggcctcggtg ctcgtttgga acgtgctatc
    35581 tacaacttta tgttggatga acatggaaaa gaaggctata ctgaagtcat cacaccttac
    35641 atagtcaacc atgattctat gtttggtact ggtcagtatc caaaatttaa ggaagatact
    35701 tttgaactca gcgataccaa ctttgtcttg attccaactg ctgaagttcc tctgacaaac
    35761 tactaccgtg atgaaatctt agacggcaaa gatcttccaa tctacttcac tgccatgagt
    35821 ccgtcattcc gttctgaggc tggttctgcc ggtcgtgata cgcgtggctt gatccgtttg
    35881 caccaattcc acaaggttga aatggtcaaa tttgccaaac cagaagaatc ttacgaagaa
    35941 ttggaaaaaa tgacagccaa cgctgaaaac attcttcaaa aactcaacct tccataccgt
    36001 gtcgttgctc tctctactgg agatatgggc ttctcagctg cgaagactta cgacttggaa
    36061 gtgtggattc cagcacaaaa caattaccgt gaaatctcaa gctgttcaaa cacagaagat
    36121 ttccaagccc gtcgtgccca aatccgttac cgtgatgaag cagatggcaa ggtgaaactc
    36181 cttcatacct tgaacggctc tggacttgca gttggacgta cagtggctgc aattcttgaa
    36241 aattaccaaa atgaagatgg ttctgtgacc atcccagaag cacttcgtcc atacatgggt
    36301 ggagctgaag tcatcaaacc ataaaaaata aggtttagct atttctagct agaccttttt
    36361 tcgtaaccaa atcagataag cacctagtac aaagaataaa atagttaggc atataatggt
    36421 ttcagccaat accaggtaat ccagaaatgg aagtttcaaa attccctgag ccatcttgag
    36481 cgaggtcgct gtgataatgg ttgggaaggt gagggctgag agggctggtt gaaaaccttg
    36541 ttttaaaatg ttgggcagac gagttaaaac aaagaaaaag aaggattgag aagccaaaat
    36601 catgacaatc aagacccaag tcggcaggct ggttcctcct actcgaacta gagaagccaa
    36661 gagtagagag aaaggagcac agtagattcc ttcttgtcca agcaaggcta gtgggagtgg
    36721 atgtttcttt aaatcgctat aaataagggg atagagatag aaggtcaaga gaaaaccaaa
    36781 actcaaggtc gcataggcaa tttcgataat acctaccaga ggataggtca aggcagccac
    36841 tgctatcccc acatagagaa ccgtccaact tggagtggca tgaaccctcc gccctgaaca
    36901 agcaaacttg atggtaaaac cagcaatcaa ggtcaaatcc aagagaaatg aaaaccacca
    36961 aatcccttgt gctaccaaag gaagataaga gaatacgcga aagacatagg tcgataaaat
    37021 catcccagcc ataggaaagg ttgccattcc tgacaaaaga gggggcttgg tcaattcttg
    37081 cttggtttct ttccaattaa agagatgcag aattagaaag taaatccata aaaccaaacc
    37141 agtcagacta aaaagatggg atagaaccgg caacgtatct aaaataagat ttccagctcc
    37201 tgccaaacct agcaaacaac ctgaaaatac taaggggagt tttttcatcc taacctccaa
    37261 taatcatgtt agtttcagta taacataaaa gcgcttaaat gaggatttaa aaaaacgaag
    37321 tccgcttatt tcagacttca ttttactcag atatgaatta ggcataaggt tgcaattctg
    37381 gattaattgg tgtattagct aagttgttgg catagttaca gaggattgct aggctgacac
    37441 caaaaaccac atccaaggca ttttgttgag tgtagccagc ttctaaaaac tcagacaagg
    37501 cttcatctcc tacacgaccc ttggtattga taactgccaa ggtaaactta gctagggtat
    37561 ccaatttagg atctgtttca attggagtac gattgcgaag agcttgaatc aagtcatcat
    37621 tcatctggat ttgtttgatg gaaaaggctg tgtgacctgc gacacagaag gcacaaccat
    37681 tggtcacggc tgccgtgatt tgcaccactt cacgctcaac gggtgtcagg ctgttgcgac
    37741 ggtggataga tgagacaatt tggtaggctt ctaaaacagt cggggcattg gccaagagac
    37801 cgattaggtt gggaatatag ccattgttgt ctttttctac tgtttcaaga atttctttca
    37861 cttctgctgg tgctgactct actgtatgga tagtaaatgt tgtcataaga tacctctttt
    37921 cttattattg acactaatat tattggaaaa tcttataaaa tcctgattcc taagtttatc
    37981 taagataaag ctttattctc tcataagatt ttcgttgtta tattagttta tcacacttcc
    38041 aatcacttgc ataatatata ttatatatca ggctgataaa aattatttat aggcaaaaaa
    38101 atcacacgag ctgtgtgatt ccattatttg tcaaaatact ttttagtttc agcaataacg
    38161 actggcgaca agaccaagag ggcaatcaag tttggcagag ccatcaaggc gttaacgata
    38221 tctgcgataa tccagaccat atccaactcg ataaatcctc ctaacaagac catgagcaca
    38281 aaaaacacac ggtagagcca gataaagcga gccccaaaga ggaactcaaa acagcgttct
    38341 ccgtaatagt tccaacctag aatcgttgta aaggcaaaaa gtacaaggaa gatggtcaag
    38401 agggcaggcc caaagtgtga aaagactgtt gagaaagctg actgagtcaa ggcaacccca
    38461 ttcaagtcac cactccaaac tccagttacc aagatggtca aaccagttag agtacaaatg
    38521 atgagggtat caataaaggt tcctgtcatg gaaatcaaac cttgctctac tggttcattt
    38581 gtcttggcag ctgcagctgc aataggagca gaacccagac cagattcgtt tgagaacaca
    38641 ccacgcgcca caccattttg aatagccatc cgaacgctag caccagcaaa tccacctacc
    38701 gcagcaaggg gactaaaagc tgaggtaaag actaaagcga ttgtgccagg gatttttccg
    38761 atattaaaga aaataactgt aagagttcct aagatataaa tgagggccat aaaaggaaca
    38821 acagtagttg aaaccttaga aatagacttg agtccaccaa agactgcaat cgctacaaag
    38881 acagacaaga cgagagctgt gatggctggc gaaatcgtcg ttgtattttg gatagattct
    38941 gtaatcgagt tgacttgggt gaaggttccg attcccaaga gagcaaccaa tactcctgct
    39001 actgcaaaca aaacagcaag tggtcgccac ttttctccca tccctagaag gatatagtgc
    39061 atgggacctc ccgctactgc accatggtcg tccttggtgc ggtatttgat ggccaagagt
    39121 ccttccgcat acttggtagc cattccaaag aaagccgcca tccacatcca aaatagagct
    39181 cctggtccac caaccttgat agccgtcgca actcctatga tatttcctgt tccaacagtt
    39241 gatgccaagg ctgtacacag agctgcaaaa ctggatacat caccatgtcc cttatcctgg
    39301 ataaaaataa gctgaaaggc cttgggcaga cgcaaaacct gcaagagtcc tagccgcatg
    39361 gttaggtaaa tccctgttcc gaccaataaa atcaagaggg gcggtcccca agcaaaagca
    39421 tcgattgatt taagcaattc taacatttcc ttctcctatc gtttcaaccc caaaagaaag
    39481 agcacatgca agatacatgt actctggaat gcttagataa atgctaaaaa gcggtctatc
    39541 ctagctctgt ccttttacct gagagtttga gcagttgcct gccttgcccc ttcggtgcct
    39601 ttacggtctc tccagagttc cgtccattta cagtcatgga aaatcaaacg attccccact
    39661 tctattaaac ttcattcggt gttggtattt aattgattct aattttacaa aaaatgttgg
    39721 cttttgtcaa tgtgtttatt agtaaaaatt agttcaacag tttttacttt ataaagtcca
    39781 gaatactgct atcctttaaa agtgacaata gtcgcaccac tgcctccagc attttgtggg
    39841 gcatagccga aactcttgac atgtttgttt ctttgcaagt atttggtaac tccttcacgg
    39901 atgactcctg ttccgatacc atggatgata tcaacttgag ccatattgtt aagcaaggct
    39961 tggtcgatga aggtatctag ctcattcatg gcttcttcat agcgcttgcc tcgaagatcc
    40021 agtctagctt gaggtcctcg cccagaagtt cgtttcacaa cattgacctg tttcttcttg
    40081 actggttttt cttgctgggc ttgaacaaga tcaaactctt tctcttccaa ggtcatctta
    40141 atcaagccaa cttgggcttc ccagcgaccg tccttgagtt gactggtcaa ggtaccacgc
    40201 tgaccataac tgagaaccac gatatcatct cccacctttg gagctcgttt tttcttggcc
    40261 ttttgaagga ccttattttt agacaagtcc actttttcag gagccaattt tttcaacttg
    40321 gccttggctt caatgatttc gtggggcttg agttgggatt tactgtggag atttttgaga
    40381 atctggtcac tttcacttag ggccatatcc acaatctcag cagcctgttc acgcgccttg
    40441 ttaagctcgg tttccttttc acgattaagc tcgttgtaga gtttttttag cgcacggttc
    40501 atcttgagat tttcttgctc cacctcacgg atattgtcca aacgtttgcg gctttccagc
    40561 gtctgctctt ctaattgctc aatgatacga ttgacgtcat tgtcctgatc gatctgctga
    40621 ctggcatctc ctacgataac ttcagatagg cctagacgtt tggcaatttc aaaggcatta
    40681 cttcggccag gaacaccctg cataaagcga taggtcgggc gaagagttgc agtatcaaac
    40741 tccatactgg cattttgcac aaaggctgtc tcaataccgt aggccttgag ttctggatag
    40801 tgggtcgtcg ccatggtctt gatttgacgc aggcgaaggt cctccagaat agccatggca
    40861 agggctgctc cctcttgggg atcagtacca gcccccaact catccaaaag taagagtgaa
    40921 tgttggttga ccttgccaag aatatccacg atattggtca tatggctaga gaaggtagac
    40981 aagctctgct caatagactg ctcatctcca atatcagcaa agatttcttc aaaaatacca
    41041 acacgacttc ccttgtctgc taaaatcggc aatcctgact gggccatgac ctgtgtcaag
    41101 cccagagttt tgagcatgat ggtcttccca cctgtattgg gacctgtaat gacaatagct
    41161 gttaaatctt gaccaaaatg gacatcattt gcgacggcat ttttgaccaa aggatggcag
    41221 acatggagca gttgaatctc ttgattttct gacagctgag gcacgactgc ttgtctttct
    41281 tggataaatc gaaccttggc acgaatcaag tccagatgac cgataatcca agcgtcatta
    41341 gcaatctcag ccgcatgagg gcggacacgc tcagaaattt cttggagaat gcgaagcatt
    41401 tcatagcgct catctgctcg cagactagca atttcttcgc tcagtttgac tacctcacgt
    41461 ggttcgatat agacggtgtt tccactagca gaaatatcat gaacgacacc tgcaatctta
    41521 ttgcggtagg tgtttttgac tggtaaaacc tgacggccat ttctgctagc aacaattcct
    41581 tccgtcaaca tctgcgcttt ttgcttgagc aagtcttgta aaacatcgcg tacctgactc
    41641 tcgctatcat gtatttttcg acggattcgc gccaattctt cactggcaaa attttcaatg
    41701 aaacccgcat cattaaaggc ctgaagattt ccttgtaatt gcggaaaatc atgtaatttc
    41761 tcaaaccaaa gggctaattc ttccaagctg acattttcca gattggcata aaaactttga
    41821 agttctcggc tggcaagaag cacgcgtttc aagagtagga actcctcgat attgagatcc
    41881 gctcccatct ccaacctctt gcagactcct gcaatttcct tagttgagag aatagtaaaa
    41941 tgcggttgct cgacgaaaag agcctgcatt tctttcatct cagcaaaagc ctgtttgatt
    42001 ttatctgctt tggcagtcgg agccagctgt ctcaattgct ccaagccctg ctcggtcaac
    42061 aaatgaggat caaacaaggc cttgacctta tcgaactcta atgtttctaa tattttctta
    42121 ttcatcttta tttccttgtc tttgaatgtc taggtgtggt agctttttac attctgatag
    42181 attcgagtca aatctgtaaa caaagggcta ggaaaactcc tgcccttttt atccgattaa
    42241 atttgtcacc cagatttgtt tgagccaact ggttgttatc ggtatgctct ggatgatgtg
    42301 ttttgcgacg atactctttt caagaggatt ttgtataact gccatgggga tggtcgccaa
    42361 gattgtcaag gccatttgca agacaaataa ggtcaccaac atggacaaga tacctgctga
    42421 aacttggaac aacttaccac ccagtttttt gctaggaatc aagtgtaaga gaagaccaag
    42481 caaacgacca atgctataga aaatcccaaa tacaagcaag tagccgatac ctgcataaaa
    42541 gaccttatcc agctgaaaga gttgatccga tgggaaaaag aaagtcccct gaccttcctg
    42601 cgaatttgca taagggagca ataaatggaa ttgctctcca agccccttat aaaactggcc
    42661 agccataaaa gccgatgcca tggctgaaat caggtaataa acctgtaaga gcaggcctct
    42721 ccgatagccg atataaaatc cccaaaccaa gaccaataga agaaggaatg aaatcataag
    42781 gaatcctcaa tcttgctctg ttcttgctta caagtcacaa gcttgtgacg gagttcttct
    42841 agctcttgct ccttatcgtc aaattcaatc tcacggctga gctgagttga taaacagttg
    42901 actgccaaca aaagagcgat tgtttcatca tctgcgctag gcatttgttc tttaattgct
    42961 tggtattttt ctgtcgcaac cttagcgatt tcctccataa aaaggttatc atgttcgctt
    43021 gtcaaggtta acgatttttt cccgaatgta aatttgaatc gatttagatt tgccataaaa
    43081 ttcacctcac gatattatac caaaattcgc taattttgtc agtttttaca aattttactg
    43141 cttttgtggt acaatagaaa ctatggcaag tatcacactc acaccaagcg aaaaggatat
    43201 tcaggctttt cttgaacact atcaaaccag tctggctccc agtaagaatc cctatatccg
    43261 ctactttttg aaactacctc aagcaacggt ttctatctat acttctggaa aaatcttgct
    43321 tcagggtgaa ggggctgaga aatacgctag tttctttggc tatcaacttg tagaggaaaa
    43381 cagggggcaa aatcttcctt taattgggac agatgaggtg ggaaatggtt cctactttgg
    43441 tgggcttgca gttgtggctg cctttgtcac atctgaccag cacgactttt tacgaaaact
    43501 cggtgtgggg gattctaaga ctctgaccga ccaaaagatc cgtcagattg ctcctattct
    43561 caaggaaaaa atccagcacc aggcactcct tctctcaccc agcaagtaca acgaggtcat
    43621 cggagaccgc tacaatgctg tttcggttaa ggttgccctc cataatcagg ctatctatct
    43681 cctccttcaa aaaggtgttc agcctgagaa aattgtgatt gatgccttta ccagtgctaa
    43741 aaattatgac aagtacttgg cacaagaggc caatcgtttc agcaatccta tcagcttaga
    43801 agaaaaggct gagggcaaat acttgtctgt cgcagtttct tctgtcattg cgcgtgatct
    43861 ctttctggaa aatcttgaaa atttgggacg agaactgggt tatcagctcc caagtggagc
    43921 tggaacggct tctgacaagg tggctagcca gattttgcaa gcctatggta tgcagggact
    43981 cagcttctgc gccaaattgc actttaaaaa cactgaaaaa gcgaaaaaac gcttagaaag
    44041 gtaagttatg aattcattta aaaatttctt aaaagagtgg ggattgttcc tcctaattct
    44101 gtcattacta gctttaagtc gtatcttttt ttggagcaat gttcgcgtag aaggacattc
    44161 catggatccg accctagcgg atggcgaaat tctcttcgtt gtaaaacacc ttcctattga
    44221 ccgttttgat atcgtggtgg cccatgagga agatggcaat aaggacatcg tcaagcgcgt
    44281 gattggaatg cctggcgaca ccattcgtta cgaaaatgat aaactctaca tcaatgacaa
    44341 agaaacggac gagccttatc tagcagacta tatcaaacgc ttcaaggatg acaaactcca
    44401 aagcacctac tcaggcaagg gctttgaagg aaataaagga actttcttta gaagtatcgc
    44461 tcaaaaagcc caagccttca cagttgatgt caactacaac accaacttta gctttactgt
    44521 tccagaagga gaataccttc tcctcggaga tgaccgcttg gtttcgagcg acagccgcca
    44581 cgtaggtacc ttcaaagcaa aagatatcac aggggaagct aaattccgct tctggccaat
    44641 cacccgtatc ggaacatttt aagagaccta agaggccgag aatcaccaat ctcagcctct
    44701 tcttctatcg tgagaagtga ttgtttattg acaaatactc aacgaaaatc aaagaacaaa
    44761 ctaggaagct agccgtaggc tgcttaaaac agtgttttga ggttgtggat agaactgacg
    44821 aagtcagctc aaaatactgt tttgaggttg cagatggaag ctgacgtggt ttgaagatat
    44881 tttcgaagag tataaaataa aaacaatccc aactctcacc tattcatgca aaggaatttt
    44941 atggaagttt atttttcagg aaccattgaa cggattattt ttgaaaatcc cagcaatttt
    45001 tatcgcatcc tcctcctaga aatcgacgat acggacgcag aggattttga tgattttgaa
    45061 atcattgtca caggaaccat ggctgatgta attgagggcg aagactatac tttttggggg
    45121 caaattgtcc agcactccaa gtatggagaa caactgcaaa tcagtcgtta tgatcgcgca
    45181 aaaccaacta gtaagggctt ggtcaagtac ttttcaagta gccatttcaa ggggattggt
    45241 ctcaagacag ctcagaaaat cgtggatacc tatggcgaaa ataccattga cgaaattttg
    45301 caacacccag aaaagttaga aggcatcgca ggactctctg ccaaaaatcg cgaggctttc
    45361 gtctccactc tccgtctcaa ctacggaacg gagatggttt tgtccaaact agccaactac
    45421 ggcattccca acaaactagc ctttcagatt caagactttt acaaggaaga aacccttgat
    45481 gtggttgaaa attatcccta ccagctggtt gaggatatca agggtttggg ctttaccatt
    45541 gctgaccaac tagctgagga actaggcatc gaaagtcagg ctcctgaacg cttccgcgcc
    45601 ggtctagttc acagtctttt tcaggcctgt atggaaacag gggacaccta tgttgaagca
    45661 cgggatttgc tggaacaaac ccttactctc cttgagtctt cccgtcccgt ggaactagac
    45721 cccagccaag ttgcccaaga gctctcctac ctgatcgaag aagacaaggt tcagcagatt
    45781 gataccaaga tctttgacaa cagcctcttt ttcgctgagg aaggcatccg cagtcacttg
    45841 gttcgtatcc ttgaaaaagg aaaacagaag agtcatgatt tagaaaccat tcaaaaacat
    45901 atcactacta tcgaggaaga gctgggaatt gagtatgata acattcaaaa acaggctatc
    45961 tgtgatgcta tccagaataa ggtctttatc ctgacaggtg ggcctggtac tggtaagaca
    46021 actgttatca atggaatcat cgctgtttat gccctgttag agggacttga cttcaggaag
    46081 aaaagcaatc tgcctattct tcttgctgct ccaactggtc gagctgctcg tcgcatgaat
    46141 gaattgacag gtttgcctag cgcgaccata catcgccact tgggaatgac aggtgacgat
    46201 gataccagtc atctggaaga ttacctagat gctgacttta tcatcgtgga tgaattctct
    46261 atggtagata cttggctggc caatcaactc ttctccaata tctcttctaa cagtaagatc
    46321 ctcatcgtag gtgacagtga tcagctacct tctgtcagtc ctggacaggt tctagcggat
    46381 ctgcttcata ttcctttgat tcctcagact cacttggaaa aaatttatcg acaaagcaaa
    46441 gaatcaacca tcgtcaccct agctagtcag attcgacagg gcatcttgcc agctgatttc
    46501 acccaaaaaa aagctgaccg ttcctacttt gaaattgcta gtggccatat tcctgccacc
    46561 attgaaaaaa tcttaggtgc cgccctcaga aatggtattc ctgcccgtga tatccaagtt
    46621 ctggctccta tgtaccgagg gacggcaggg attgatgcta tcaatcagct catgcaagac
    46681 ctgctcaatc ccccacaaaa agatcaactc agttttgaag ctcctcagtg ccactatcgt
    46741 aagggagaca aggtcattca tttggttaac gatgctgaaa tcaatgtctt taatggggat
    46801 ttaggagcta tcacagacct gattcctggt aaatacaccg aatcgaaaca agacgagatt
    46861 gtcattgatt ttgatggtaa tgaagtctct tacccccgta acgaatggta caagattcgc
    46921 ttggcctatg ccatgagtat tcataagtct cagggaagtg agttcccagt tgtcatccta
    46981 ccgattacta gtgctagccg acgtatgctg gagcgaaatc tcatctacac agccattaca
    47041 cgtgccaaaa gcaaactcat cttactaggc gaattacaag ccttcgacta tgctacccaa
    47101 cacatcggaa ctgcccgaaa aacctatctg attgaacgct tcagtgatct actggagaat
    47161 gttgaagaaa agcaacaagc tgtctctgaa acagtcacat caagtgcctc tgaacaatcc
    47221 tacatcctaa ccgaagaaaa ctgggacagc attccagcca tgattgggat tacagacaca
    47281 gacctcaaag agatttttgg aaaatagtgc atcagaaaaa ccaccaattc aggtgggttt
    47341 tctgtttatt aagattattt tactgttgct gtgcttgtaa tcaattcaac agcttttttg
    47401 atagtgatat catgtttcaa catgtcagct gaaagcaagt tttgaacttg tgcaacttcc
    47461 atgttgtagt ctgctgccaa ttgctcaact tctttttgga tttcttcttc tgaagcatca
    47521 aatccttcag ctttggcaac tgcttcgata acaaggttag tcttagtacg tgactcagct
    47581 tctgcttggt attggttgtg aaggtcttct tgagtagttc cagtaatttg gaagtacatg
    47641 tcagggttga tcccttgacg ttgcaagttc ccaaggaatt catttactga acggtgaact
    47701 tcttcgtgga tcatttcttc tggaagttct acgatttcag cattttctac agctgtatca
    47761 attgctgcac cttcaactgc atctttgtaa gcttcttctt tagcagcagc caattctttg
    47821 cggtattttt ctttcaagtc agcaagtgtt tcaacttctt catcaatgtc ttttgcaagt
    47881 tcatcgtcaa gagctggaac ttctttagct tttacttcgt ggatagttgt cacgaattta
    47941 gcttctttac ctgcaaggtc ttctgcttgg tagtcttctg ggaatgttac gataacatca
    48001 acggtttcgc cagctgagtg acctaccaat tggtcttcga aaccagggat gaattgacct
    48061 gaaccaagtc caagtgagaa gttttcacct tttccaccgt caaattcaac accgtcgata
    48121 gaaccaacga agtcgatcac aacagtgtcg ccgtcttcag cagcagcttc cttgataacc
    48181 aattcagcca ggttgttgcg ttcacgttcg atacgctctt cgacatcagc gtcagttact
    48241 tctttttcta catcaactga tacttcaagg tttttgtagt cacccaattt tacttcaggt
    48301 tttgtaacaa cttcagcagc gataacccag tcttgacctt tttccattga agttacgtca
    48361 atttttggtt gggcaaccac ttcaagacca gcctctttta cagctgcttc ataagcgttt
    48421 ggcaaaagag cgttcataac gtcttggtaa agtgattctt caccaaattt tttatcgaag
    48481 ataggacgtg gaaggtgacc tttacggaaa cctggaacat taagagattt cttcactgac
    48541 ttgaagacac ggtccaattc tggtttgatt tggtcttgag agatagtgaa agtcaagaca
    48601 ccacggtttg tttctttgtt ttcaaatgat acagacattc tgtcatttct ccttaaaatt
    48661 ttttaaatac agtctattat aacattttct ataaataaaa tctagactaa tgcagacaat
    48721 tatagacaac aagccttttt cctaactaaa acactcataa agtatagaca attttagact
    48781 actttggata tcttttggtt agtcattttc caaaactaat ttatctaatt tttaagttat
    48841 atttgacttc atcttctcag taacatgagt ataaacactt aaaatctttg tttcattagt
    48901 ataatctaca cgatccatta tcgctgttat aggtacttcc aattcagcaa gcaaacttat
    48961 atgcgaatat ctaaaaacat gagcagtcaa tacaaaatca tctttcttaa tttcactttt
    49021 cctaaacctg tttttcaaaa agtaattgaa acaagttatc attaagggag aaccattatt
    49081 tgtaaatata taatcacctt cttctaaaag aagatattct tttaaaattt ctaacactct
    49141 atcactaata ccaattactc tatatgaagc taaacttttc ggactatcca aaaacacttc
    49201 gttattggac gattacatta tagtaaaacg aaataagaac aggacaaatc gatcaggaca
    49261 gtcaaatcaa tttctaacaa tgttttagaa gtagaggtgt actattatag tttcaatcta
    49321 ctatacaaga cgaaattgaa gaaatctttc cattataaaa cgcatattat caaggttttt
    49381 aaacacctaa taatatgcgt tttttcctat tttaaagact tttatccacc tattatacaa
    49441 agttaagtat ccatcacagt ttacaattag ccttcattac ctaacccatt caccatcctt
    49501 caagagagta acctttaaga accgaataaa taaatggcaa atttcgttca tttaatttaa
    49561 ctgcactgtt aatttgatta gctccatctc ctaaagtatg cactcctact acacggtgac
    49621 tagcatcata aactgtagat ccactagacc cctctaaagt atcaacttgg taatccaaga
    49681 acatgccatc atcacttaaa acttgttttt tatctgtata catttgatga actttaaaat
    49741 tatatgatgg atagcctgtg atagtcacag ttattcctgt caaatttttt tgactagtag
    49801 gaagacccaa agtccctaat tttgcaccaa tgggctcttc tagaattaat aaagccaagt
    49861 catattccct tgcatcctta gaatttaaat ttctaaattc cttcaaataa cgaacttcct
    49921 ttactttgat ctttccaaat ggttcttgac ttggactaac agtcggaaga acataaatat
    49981 catccgcttc tttcccatag tcatgtctgt agtagttgtg agccgctgta attaacacat
    50041 tatctttaat aaaattcgca cttcccctcc cttctacatt cattccatcg gctactccat
    50101 aatacttaga taaaaccata gctgaagtcg aatacggata ctgtaaagta ttatcaattt
    50161 taatcaaatc atcattaccg ataatacttc tgattgcttt tggtagtatg aaccatatgt
    50221 tggtgaaatc tcagataatg aagaatcatt agatcctgga cctttttcta gtgtctcact
    50281 tacctcatat tcttcacccg tactagaaat aacactcaaa gcagatactg tcgataactg
    50341 gctagccaat aaagtactcg caataattga aatacccaat tttttataaa cagttttctt
    50401 cattattgta tcctcctaat gtaattatag cgtactattc taaatttctt aatctactat
    50461 agaatcaaga aatctaccac cttctttaaa taccctccat tatcacataa acaggtaaac
    50521 ttttcaatta atgactgcgc ttttcaatca cgctagaggt acttacttgc ttctttgata
    50581 ctaagttcag ccattctttc cttgtttttc tcaataaagc atgttaccca agtgggattc
    50641 gttttggagt agtctcgcag agtccagcca atggctttat tgataaaaaa ttctgtttgg
    50701 ttcaagttat gaagtagaat cttttccatt aattgagtat tggtcttctc ttttcttaac
    50761 aactggtggt caatagcgac acgtctcagc cagatattat ctgataggct ccattttata
    50821 ctcaatgaaa atcaaagagc aaactaggaa gctatctgca acctcaaaac actgttttga
    50881 ggttgcagat agagctgacg tggtttgaag agattttcga agagtattaa gattatttct
    50941 tctagttcag ggtgttcata caccaaactc cctactactc gatctaggat atctaccgtg
    51001 tcccaccagg attttgtcac gactaactgc tctagcttag gcaaatcggt ttcctttaga
    51061 taagactgca ttgctttcaa atagttagca gccacatatt ggtattttct aggctccttt
    51121 tcccagcaag tgtctgcaaa atcccaatcg ataatctttg tttttttcgc ttctggtaaa
    51181 tatttttata gagtttattt ctttcaggca ccgcaatacc tagaaaagaa aattgatggc
    51241 gcatataggc ttccatgggc cttgcttttt tagagtcttt tgctgcttct agctcctcaa
    51301 gtaaatctgc taaactcatc taaaactcct cttgccccac caaatggtgc tgaaaggcat
    51361 agacagccgc ctgggtacga tcgctgactt caagtttggc aagaatattg gacacgtggg
    51421 tcttgaccgt cttgagagag ataaagaggt catctgcgat gcgctgattt tcgtagccct
    51481 tggcgatgag ttggagaaca tctcgctcac gcgcagtcaa ttcttcatga agttccatat
    51541 gattgcggtg gtattcaacc ttcttgctaa cctcttgctc aatggccagc tcgccagcag
    51601 ctaccttact gacggcatga agcaattcat ctgcactaga agtcttgagc atatagcctt
    51661 tggcaccagc atctaagact ggcatgattt tttcattgtc caaataagag gtcacaatca
    51721 aaatcttggc ttcaggccat tctttaagga ttgctaaggt cgcgtcaatc ccattcatct
    51781 caggcatgac aatatccatg acaatgacat ctggacgcag ttccaaggcc aagtcaatcc
    51841 cttgagaccc gttggacgcc tcacccacaa cttctacatc gtcttggagg tcaaagtagc
    51901 ttttcaagcc caaacggacc atttcatggt catctactag taaaattttc atctttactc
    51961 ctttatcatt ccttatctaa caggggaata cggatatcaa ctgccagccc ttgcttggga
    52021 gctgttaata actgaaccgt ccctgccata tcttcaaccc gctccttgat atttcgcagt
    52081 ccataactca agtcgtctaa gcttcctaac cggaaaccaa tcccattgtc caccaccttc
    52141 agttgcaatt caacatctgt ctgatagagg tagacatcta ggcaagatgc ctgggcatgg
    52201 cggagggtat tgctaatcaa ctcttgcagg atacggaaga tatgctcctc gattttctta
    52261 ggcaatttcg tcatattctg cttgagacta accctaagat cactcttgtc ctcaagctct
    52321 tttaagagaa tctgaatccc ttctatcaag ctcttctgct ccagttcaac tggtcgcaaa
    52381 tgcaagagca aaacccgcaa atccttctgg gcagtttcta aaatagctgt gacactctgc
    52441 aactgggtct gcatcttttc tctatccaat ttcaaagcct gctgactgat acccgataaa
    52501 ataatgtggg ccgcaaacaa ctcctgactg actgtatcgt gcaaatcccg agcaattcgc
    52561 ttccgttcct tctcgataat ttcctcttcc tgatcaaggc tatgattttc agctttttga
    52621 agagcctctg tcaaaaggtt aagtttacct gataaggact tgaaactggc atccaaatct
    52681 ggatctacaa cctgaactac ttcttgccct gccaataaac gcttgagatt agcctgcatt
    52741 tttcttagag aaagctcttc gatccctcgc caaaacaggg ctaagagaca ggtcatggac
    52801 atgctgaaaa ccaacaataa aaagacaaat ttttctgttt tttcgacatc gtacaaaaag
    52861 atagaccagt caaaatcaag tatttccagc aagctgtggg agaaaaaaaa gacaaatagg
    52921 aaggaggtga gagcaataat gacataggct tgttttttca tcctctaacc acctccacat
    52981 caccaatcat agtggtcaag aaaatcttga cactcttgtt actcttgaga tagtcttttg
    53041 tttcttgatg atagtgttca ttgcggaggg ctcgcttggg ctggttgaaa aaagtcaaat
    53101 ccccatagag acagttaacg ctgagactga cttccacatc tacaggtacg atgattttgg
    53161 tcgttcctac catctttctg aggataatga cattgtcatg attggttaag atgactctct
    53221 ccagatgaat agtgtccttg cccatgaagc gaaagagatt gatatcatca aattggcaag
    53281 tctggtagct tgaaaaatga tgaagatttc caaaccaacg atttttctcc ttcttaaccg
    53341 tcacaacctc ttcaaaaacc aaattggtct gctctttttc ctggttcatc atcgggtaaa
    53401 gaagaaagag actatagata accgcaacaa aaatagctag aatcacaaaa ggattgagca
    53461 taacgatgaa aaagaagaga atggttgccg ctactaaaag aagattattc ccctctttac
    53521 cagtatagta gcgaatcaaa agcaaaaaga ggaatagtat cagcagaaaa cgcgaaaaat
    53581 gctctgatac catcaaaatc agagctcctg tcagaagaca ggcttcgata aataaaaaga
    53641 ttttaaattt tctcataggt tcatcctctc ccttctattt tatcacaatt caaaaaagtc
    53701 acctcagtct gaggatggaa aaaaaggcgc tggttacgcc tttttcatct gatcctttgc
    53761 ttcttttaat tttccataaa gaagatagtc tactttttgt agatctgcta tggtggcaca
    53821 gttaagggaa cacataatca agcgtagatc tgctttccag ccttggacaa tgccaatcac
    53881 ttcttcaact gtgtaggttt caaccaattc cagaacggtt cgtgacaatc ccacagcctt
    53941 agcaccaaaa accaagcact taatcatatc cagcggattc cgaacccctc cactaaccaa
    54001 gagttcgacc ttatctttcc attcttgggc attgagaagg gcctgcatgg tagactgacc
    54061 ccattgattg aggtaatcac gctggccact acgacggttt tcgatatagg caaagctggt
    54121 gccaccacga cccgataggt ccactgtacg aacaccgaat tcataggctc tttcgattgt
    54181 cttggcatcc attccaaagc ccacttcctt gaggacaata ggaacgggaa tttgcttgct
    54241 ataatctgct agatgcgatt gccagcttct aaacttcctt tctccctcgg gcatgagtaa
    54301 ttcctgcatg acattgacat gcacttgcaa tagaacagga ttcatctctt ctacagtctg
    54361 aagtcctaac tcgacaggtt tgtccaatcc aatattggtt ccaaggagga gattgggatg
    54421 actagacttg acagaaaaag aatcatccgt tggatttttg agggctgcgc tataagaacc
    54481 cgttacaaat aaaataccac aggattccgc cacctgagcc agcttttgat tgatttctct
    54541 tcccttatta cttccaccag tcatggcatt gatataaaaa ggaaagtccc actttcgacc
    54601 agcaaactct gtcgaaagat cgatttcatc cagattgtaa agaggcaagg aagaatgaat
    54661 cagctccacc tcatcaaagc tattatagga acttttctgc tcaagggcat agaggatatg
    54721 ctcgtcctta cgatttgtcg tcatgtccta tcctttcttg atataagagc tcaatcccca
    54781 gatcggccca acgatttttt aaggttttgg ttgattgcgc atcaaaactc agggcgatgc
    54841 cacagtcacc accaccagca ccactactct tggcaacggc ctgcaaatct tgactggctt
    54901 ctttcaactg tctaagcaaa ggcgtgtaaa tatctgtact caagccttct aaaagcttgc
    54961 tggctacttc tacttgctcg ataatctttt ctgatttccc ctgttccaag gcttcgacca
    55021 aagaaaccac cgtttctttt gaggaactta aaaaattttg attgatattt tgcttgattt
    55081 gctggaccat gtgactcgat acagccactt ccttggtcca tcccactaag aaatcacatt
    55141 ctaaagttgg tttcacttgt gaaattgaaa agccccaatc acgctccaga actgtcgcca
    55201 agttttcttc ttctaaccaa gcagccacct tctggcgatc aaatgactgg tagagaacca
    55261 aatcctctgc cacaatacag gcaaggtcgc ccatggaacc attgtctcct cgcttgagca
    55321 agacagcgct agtcagcttg aacaagagct cctgatcaac agaaacatca tacagagcca
    55381 gtaaagcctt gacaaccaag acaacgacgc tgccactaga acctagacca aactttttcc
    55441 cttctcgttc cattttgcca cagatttcta gggaaaaaga tcttaaattc tgaccgcgaa
    55501 cagcgaggaa gtctcccatc aaagcaatcg tttcttgaat caagctgtag tcaggattgg
    55561 gcgttaagtc cactgcgaaa tcaaacatat ctgaatagat acggtagctg tcagaaaaag
    55621 caatctcagc cctcatatag atgggaatat cctttatcaa agctaactgc cctggctcta
    55681 aaatagcata ttcacctgcc caatagagtt ttccgcaagt tttaacagca atcatcttga
    55741 ctcaaatcct ttgtttttga cacaatcaag cgataacgct gaccgaaaat ttctgacaaa
    55801 tgctccaagt ctttctcctg acaaaagacc ttaacattgg gaccagcatc catggtaaag
    55861 tagcaggcct ctcctttctc acgaagctgg cgaacaaagt ccatagcctc ataagaggca
    55921 tccgtcagat aagaaaaggc tggactagca gtctttgtcg tagcatgcat agccagggca
    55981 tttttctccg ttaattctcc aatcttggca aaatcatttt ccttgagata aatcagcata
    56041 tcctgatagt ccttctcaga ctgacgaacc cagtcgtcaa aagtcgtcga ggtttccaca
    56101 caaagtttca tcccgtcacg gctagagatt ggttttttct tgtcctctag caccaacata
    56161 atcatagcta gtttcaagtc tgtctctaca gagtaaattt ctccactatc cttatcccag
    56221 gctcctagcg gtccataaaa actccgagaa gaagagcctg aggcaaactt ggcttcctgc
    56281 gctaactgac ttctatccaa tccaagcttg aaataagcat tacaagcctt gaccagggcg
    56341 gacaaaccac tagaacttga ggacaggccc gctgcagtag gcatattgtt ttgagtatcg
    56401 atacggacaa agccctcacc agctggacga taacggtcaa taatcttact catcttggca
    56461 tgctcgacct cattttgtag ctgaccattg atgtaaaatt cgtcagctat tacattggct
    56521 ggtaaaggcg acaaggtcgt ctctgtatac atattttcca aagttagaga aatactgcta
    56581 gtagcaggca ccatctcttt ttcttttttc tttccccaat atttgataat agcaatattt
    56641 gcgtaggaac gtactgtcac aggctctcta tccatgtctg aacagctcct ttctcttcta
    56701 atctttctgc tagttcttgt gcgtgtgtca aattggttac caaggctatg atacaacctc
    56761 ctagcccacc accgctcatc ttggcaccca gagcaccatg gctaagagtc gtttcaacca
    56821 aaaagtctgc ctcagggcta ctgactccaa tttcttttaa atgtaaatgc gcttgactga
    56881 ggatttgtcc cagtccttca gcatcttttt gtgaaatcgc aacttctgct tgctgggtta
    56941 attctcccaa ggcatgcaaa aacggtaggg catccttgcc cttattttga accacttgga
    57001 tggcttcacg agtatgacca taaacacccg tatcggcaat caccaaatag gcggataaat
    57061 ccatctcaag ttctgtaaat cctacgttct tgataaagcg aataggttgg tcactgagac
    57121 aggtcttagc atccaaacca ctaggattca tatgggcaat catctcagct cgattgacca
    57181 agatttctag tacatcatga ggcagatcag cctgatagta gtcaaatacc gcacgaatgg
    57241 ccgctatgct gatagccgct gacgaaccca tcccccgttt ctcagggata gccgagtcaa
    57301 tctcacaacg aatgcaggct tctgtgatat ccaaatactc cagtgaggca taaaccgcca
    57361 tggacaaggt atcctcctca taaaggcgcc aaggactctc tgcaggaact accttacagg
    57421 tcacctccac ctccaaaaga ggcagggaaa tggcaggata accgtaaacg accgcatgtt
    57481 cccctattaa aattatctta ctatgtgcct gaccgacacc aacttttttt gtcatttttt
    57541 ccttttacta gacgaaaaaa cgtcttattt ttcatacaag tattaattct ttcctatcta
    57601 ttttattata ttttcacaaa aaaagcgatt gtttccattc acaatcgctt ctttcattat
    57661 tgaacccatt cgccattata gttgacagaa tagccatcta cggtcgtatt cactgccaag
    57721 gcacctgagc tataagcgta gtaccatctg ccattgacct ggaaccaacc tgtcgtcata
    57781 gaacgacgaa agaaactcca taccgttaag taaagaggaa agtcgtgagg gatcatgcgc
    57841 cattgacaac ctgttttagt gacgtacaaa gtctcattaa caagtactcg tttcggccat
    57901 ttataggtgc ggtgtttgga gaaatagggt tcaatcttag cccattcttg atcgtttaaa
    57961 tcagtatcat atgctttgcg tgtcataact ctagtttaac attttttgtg aatacaagtt
    58021 ctaaataatc gaccacgaaa atttcttaag tggaaaacgc cttatgaagt atgctacggg
    58081 aaacttatgc acttaatttg acaattcaag atgtaaaaat atatactata gtagattgaa
    58141 actagaatag tacacctcta cttctaaaac atttttagaa atcgatttga ctgtcctgat
    58201 cgatttgtcc tgttattatt ttattttact ataatatttg acaagttatc ctaaaagtat
    58261 tattatgttg ttgtgttata gattgattga atctaactaa aggatcctat tcaattacta
    58321 gaactatcac atactcaagg tcagctcaca gatgagcaac tattttggtt acaatgtcta
    58381 ctaaatttaa gtcaaacaaa taatttagtc aaaattaaaa aaatagagga acataaatat
    58441 gattacaaaa cagaatgtaa tagtgttcta cgatttttac tagataaaac tgtaaattct
    58501 gaaggaagga tcacttcttc aacagaattt ggaaatttcg taagtaattt atcattccaa
    58561 cacggaatag ctggactact gtttcctcta aataaattgt accccccaga actggattct
    58621 aaaatactct ctatcatcaa gaaggcagtg acaattagaa cgacacacat atgaatatca
    58681 atactcactg ctatttggtg atgcaggcta tctatggtta ctcctacatt tattttctat
    58741 cagtaaaaat caatactatc tacaattagc aaacgtcacc gctaagaaat taatagagaa
    58801 ttatgatact ctagaagaaa tagactttgc attgggaaaa tctggtgtcc tattatcatt
    58861 aataaaatac tatcaattta ccaatgacaa tactcttaaa actttcatcc acaatagtat
    58921 atggggaaat ttatcattat ttcctacaaa gagatacagc caaagaaagc attttagact
    58981 atagctttgc tcatggatat tgtgaaattg catatgcttt atttgcctat tctaaagtct
    59041 tagaaccttc tatgttctat aatgatctcc atacattcca tgctgaatta aaaaaattat
    59101 tagaaaaagt tacttctaat actgaaaatt taggaaattt acaactttct tggtgcgaag
    59161 gaatttccgg aataatctta tatctttgta tgtacgattg tgacggaaac aaagatatta
    59221 ttagtaaata tcaagaattt gtttttaacc atcatctaaa aatgatgaca ggatattgcc
    59281 acggaataac tagcttacta caaaccactg tctacaatca aaacaaatta ctgatgaaaa
    59341 aaatccaaca ggtaatttta gcatgttctg aacgagatga tcacggttta ctgatgtttc
    59401 aaggagatag tggtaaagca gatttgtttg acttcggaat aggaagcagg gggtatattg
    59461 gtgtctatta aataataaat tcccattcga tgtgcaaaca taaggagaaa tgtatgaaat
    59521 tattttggac aaacaacata tatagacagt tgctgctaaa cagctgtttt tcatcattcg
    59581 gcgacagtat tttctacctc gccattatca attatgtggc tcagtacaat ttcgctccgc
    59641 tagcgatttt actgatttcc atttcagaga tggttcccct actatcgcaa ctctttctcg
    59701 ggattctagg agattttcaa gaaaatagag tcaaacacgc actctgtatt gccaaaatca
    59761 aaatcctgct ctacgctatt ttgacagtat ttctcgtctt gtcgcccttt tcattagttt
    59821 cagtcattat gattgtcatc atcaacctca tctctgacac cttgagctac ctgtctgcct
    59881 acatgatgaa cgccctctac atcagtgtaa ttaaggatga cctgcatgat gccatggggt
    59941 tcaggcagtc tctgatgagg gttgtccaca ttgtcgccaa tctggctggc gcattcctta
    60001 tcaatgttat aagtattcaa actatttccc ttatcaacac tctgactttt gtcattgcct
    60061 ttttgggcct gtatgttatt cgacatacct tgtatgaggt tgaaaaaagg attgaaatgt
    60121 cacatacagc actgagtttt aagaaatatt ttcaacatct taaacagtcg ctggctgtgc
    60181 tcctgaggtt aaaagatacc gtcatactac tgtttctgac gaccagtatg attgccatct
    60241 tggatgtgtc ccctcggctg attgccctcc gcttcatcca acagacacta gcacaactga
    60301 gcattgggca actcttcgcc ctgctctcca tcatcatgtc ttgtggagct atccttggca
    60361 atatgaccag cagtaatcta tttaaaaata tccgtttcac gcacctcttg gttttctgtg
    60421 agatttccct attggctcta ataactagta tcctttgtca agcctatatc gtaattttcg
    60481 tgaccagttt catcagttct acgattatcg gcattctcag ccctcgccta caagcagctg
    60541 tctttgccca tatccccagt gacaagatgg ggacggtggg ctctgctctg agcacagtag
    60601 acattctcgc cccgtccctg ctctccctat tagccctatc catagcatcg ggcgtttcgg
    60661 tgcagttagc attgatattt ttgtatctta ttttgattgc tcttatcttt tgtcaatggt
    60721 tagtcaagtt caacactcat aactaacgaa aaagcatgtg tagatttcac atgcttttaa
    60781 tctccccaat cgtcaggtca agtacaacaa agtcacttct ttgattaagc gagtgttcta
    60841 atataattat aagcgccctg tcattaccga acccattcgc cattatagtt gacagaatag
    60901 ccatctacgg tcgtattcac tgccaaagca cctgagctat aagcatagta ccagttgcca
    60961 ttgacctgga accaacctgt cttcatgtct ccattacctg catttaggta gtaccaagtt
    61021 gaaccatctt gataccaacc agttgccata gctcctgatg aacggagata gtaccatttg
    61081 ttcccaaggt tttgccaacc tgttttcata tcgccatttg gctggtctaa ataataccaa
    61141 gtggtacctt cctgatacca gccagtggcc attgctcctg aggaacggag gtagtaccat
    61201 ggagcaggtt tgcttctatc aatgtcaata gtaacatgaa aatcttgagt ccaaccctta
    61261 gcaacttcac caattggtag tggattgtaa gaatcatcac caaaatttcc tagcttattt
    61321 aaataatacc aattgccgtc ttcataaatc cagcctgtct ttacagcata attattatca
    61381 aaataataag ttctcttttc agcagataga ggatattgtt caccgtatac ttttcctgta
    61441 ttttcagacg tttttgcctc tagtacttgt ttatctgttt gttctagcaa agcaccatct
    61501 tgtccaaaat agtaccactt ctcctgaagg ccaatttcga agactggacg attatcaaat
    61561 aaatcatcac gataacctgt tccaggaatt tctaagtatt gccaaccgac aaccatctct
    61621 ccaatatcac caaaatagta agttttaccg tctattttat gccaatagat tgctttatga
    61681 tcatctttta cataatattt tctattatcc ttatcaacaa atcggccacc tgtggtattc
    61741 gcaaataccg tatttgtagc tagcaaacca aagaaaaaga ctgctagtag aacttgcatc
    61801 atttttttca aaagtttcat ttatatatcc tcctaaaacg attgtagcaa agacacgact
    61861 gcatgtcaat atataaaaac tcctcaaaaa agcagttaca caactgcaac tgcttttcta
    61921 ttcttgcatg gtgtaaccaa caccacgcac ggttttaatg tagctttttt gtcctttaac
    61981 atcaagcttg ctccgtagat agcggatata gacatctacg atatttgtct cggtcgcact
    62041 ttcatacttc caaacacttt ccaacaattg ctcacgagtc aatactttct tgcttcccat
    62101 aagtgtcgcc aaaaggtcat actcacggcg ggtcagagca atcatctctt caccacgata
    62161 aaccgtgtga tgttcaacat ctatcctaag attgcggtag gtccttggaa ctttcatcag
    62221 actgcagtgt tgatcaatga agtcccgacc tcggaagatc gccgaaatac gcgctaccag
    62281 attttcgata aagactggct tatagatgta tgaaactgca aaacgctgaa caacttccag
    62341 ctcttcttgc aagtcttccc aatgatctaa aatcatgatg actgaggcag gtttagttcg
    62401 actcaatttt tctgcaaaat cctgagccat catatctccc agattaacgt tcaataaaat
    62461 caaatcatag tctgtctgaa gagccatgga aagggctttt tgcccctcct ctaccagatc
    62521 aacccgatac tgctctttct ggagttccaa acttaaaaaa tgagctagat ttcgttcttt
    62581 ctcaagtaat aaaatccgtt tccccatggc tgacctactt atttttcgtc ataccaagag
    62641 tagtggaagg ttccttcttt gtctttacgt tggtaagtgt gagcaccaaa gtagtcacgt
    62701 tgtgcttgga tcaagttagc cggaaggtca gctgaacggt agctatcaaa gtaagtaata
    62761 gctgctgaga aagttggcac tggcacacca gcttgaaccg caagagctac gatatcacgc
    62821 actgcttgtt gatacttagc agtaacatcc aagaagtact cgtccaaaag aaggttggca
    62881 agatctgcat cgcggttgta agcatctgta atcttttgca agaaacgaga acggatgata
    62941 cagccatcac gccagataga tgcgatatct gcaaatggca agttccagtt gttttcttta
    63001 gaggctacac gcaattgagc aaatccttgt gcgtatgaaa tgatttttga gaagtaaagg
    63061 gcttgacgga ttttttcaat caattcagcc ttgtctcctt caaagttgaa ggcagctggt
    63121 tttggaagca ccttgctagc atgtacacgt tcttctttgt aagttgaaat gtagcgtgca
    63181 aacactgact cagtaatcag tgacaatggt acaccaaggt caagagatga ttggctcgtc
    63241 catttaccag ttcccttgtt acctgcagca tcaaggatgt agtctacgat tggtccatct
    63301 tggtcttcat cgtctttacg gctcaagata tcagctgtga tttcgatcaa gtagctatcc
    63361 aattcaccct tgttccactc agtaaagatt tcagccatat cctctgcaga aaggcctagc
    63421 aagtgttgca tcaagtcata gctttctgcg atcaattgca tatcaccgta ctcgataccg
    63481 ttgtgaacca ttttcacata gtgaccagct ccatcaggac cgatataagt cacacatggt
    63541 ttgccatctt ctggtgcttt agctgagatt tcttcaagaa catccgaaac caattcgtag
    63601 gcctcttttt gtccaccagg catgatagaa ggaccttcaa gggcaccttt ttcaccacca
    63661 gaaactccag taccgataaa gttgatacct gagtttgcca attcttcatt acgacggatg
    63721 gtatctttgt agaaagtgtt tcctccgtca atcaagatat cacctttgtc aaggtgtgga
    63781 agaagggctt ggatagtagc atctgtacca ggtccagctt gaaccatcag catgatacga
    63841 cgaggttttt cgattgagtt tacaaaactt tcaacgtcat agcttggtac aaaattcttt
    63901 tcaggatggc aagcaatcac atcttccgtt ttttctttac tacggttgta gatagcaact
    63961 gtgtaaccac gtgattcaat attaagggca aggttacgac ccattacggc catacctacg
    64021 acaccaaagt tagcttttgt catttgacac tcctcttgtt tcatttgttt tattttatca
    64081 tttttacttt tgaaaagaaa gaattttgta acgactgctt agtagtccaa gtcatccgca
    64141 tgaccagcgc catttccgac aaagtagcct gtcttacagt taagggtaaa gagataggtt
    64201 ccatctggct tatagaggtt gtaataacca ttgccgttaa cgatattgac acgttcaagg
    64261 atatattggt ctccagtgat atagccacgt gaacgtgaag ttgctagaat ttgttccaaa
    64321 acaccatccg caaaatccca ggcagagtta ttactatcat caatagcaga ctgattatag
    64381 gcaacacgac tggcacttct ttgaacagca acccctgcac tcgagagacc catattgact
    64441 tcactgcgac tacttctagt ctcatttgaa ctacttggtt ttggctcact agtcgtattt
    64501 gaacttgctt gacttgaact tgagctgcta gtttgacttg aacttgagct agaagtactt
    64561 gtttgctggc tcttaccaag actgatagcc ttatctagca ctttatcaag ctccgtattt
    64621 ccagttttaa tatccgtaaa tttagcatcc gatttggctt tggcattggt atccaacaca
    64681 ccatccacaa tagctggttt ttcaaattgt gcattgacat cttgaatggc cttgatttgc
    64741 gttactagac tatcatattt agatttggca agcgtatgtt cacgactacc ttccagctta
    64801 tcaagtaaag tcttgagttg actcagttta tcaaactggc tatttttcaa agccgtttta
    64861 ttgctatttg tgtaaaaggc gtcataaagt gtattaaaat catccacatc cgattgattg
    64921 gctgtagttg attgagaagt ttcaatttcc ttggtcgaac gagccacttg acgatagaca
    64981 taataagcac tgacacaaat aattactgat acaagcgcca aaacggtcaa aataaagcct
    65041 tttttacttt tcttttctga gtcctcttga tccagacgag aaaggttttg ctcctcttcc
    65101 acttcttcag tcatttctgt tggattttca tcatctagca atagaggatc caagccttgc
    65161 tcctcatcgt ccagaggcaa gggtagtaag atgtcttcag aagatggaac ttcctccgaa
    65221 gaagcttctg acttctctac agcctcacgc atctcttgaa ttaaatcatc cagactctga
    65281 gtttcaacga actcctcttt tttgtattga cgagtcgcaa acttatccgc ctcaatttca
    65341 tctctgtgtt gcttaacata cttgtccaaa atggaatcct caggcaagac tcctgcttcc
    65401 acttcttcat ttttacgaat agcttgacca actgttagct cttttgcttc atcaaaatca
    65461 aattgcggtt cttgaccttc ttttttatga cgatttcgtc tttttttact catgagtatc
    65521 cctttctatt ttacctcttg ccgtttgaca cgctgaccaa aatattgata tagatccact
    65581 ttcaaggttc cgttgtataa cttacgcttc ttatctgctt ggctaccata cttgctttca
    65641 aaagcttcat cactagtcag gataaatttg ctccaagttt tcagcggtgc aaatacttgc
    65701 cccatctcag catagagctt ggtcactcct gcatcatctg acaaacgttc accataaggc
    65761 ggattggaaa tgattactcc attgatttta tcggaacgta aatcctgcac gcgcatctgc
    65821 ttaaaagtaa tatctcctgc aacaccagct gcctgagcat tggccttagc aatttccacc
    65881 atgcgagcat caatatcaca gcccatgata tccaactcaa gcttacgttc tactttttta
    65941 gccgcttctg tgcgcacttc ttgaatcaag cgatcgctga tccagttcca ttcctcaaat
    66001 gcaaaagagc gacgaagacc tggcgccatc tttctagcaa tcataactgc ctcaatacag
    66061 aaagtccccg aaccacaggt cggatcaatc aaaggcttgt ctggatacca gttagaaagt
    66121 tgtaaaatgg ctgctgccat attttccttg ataggagcgc cacctttttc ggtacgataa
    66181 ccacgtttaa agaggctaga cccggtcgta tcaatcatga cagttgccac atctttgaga
    66241 atagagacct caatcttaaa ctctgggcca ttctccatca gaggaacccc ttctgggcga
    66301 gcatagtgtt tctgcaattt cttgacaaca gctttcttag aaatagcctg aacactgggc
    66361 tcattgtgaa gtttggactt aacacattta gcttttgaaa tcggtaaccg agctccaagt
    66421 ggtaaataat tttcccaatc taaagcgaaa actccctgaa atagctcttc aaaagtctta
    66481 gctgggaacg ttcctacgat aattttgata cgatctgctg cccgaagcca aaggttggtt
    66541 tcgataatag ctctcacgtc tccttgaaaa cgaacacgtc cattttcaac ctgacaatcg
    66601 tagcccaact ctcgcacttc acgaccaacg acagcctcaa gccctgctgc cacagttgca
    66661 attaaattaa attctttttt catgttacag tcttgcaaga cctttctata tgtccacttt
    66721 aaaaaatgag gttgagaaaa tttctcagcc ccaacctata tgcaattttc tataagccat
    66781 gttttgttcc agaagtgact aggaccactt ccttcgataa tcatctgtct accactaaca
    66841 gctctagctg atagcagtca gaatactacg ttcgtttccg catactccat gccccgacca
    66901 aaatttgggt tgctagcttg aggggtttac cgcgttccac tctctctgtt tccagaaaga
    66961 ctccgtcact gtggcacttt caagcctact ctggcctatc caaggactta gccatttcaa
    67021 ctgccgtaac gatttctcgt ccctaggctt atggtttcgc ctagcacaaa cactacaggc
    67081 atcacagcct gtgctagcat ggactttcct catgagaagc aattctcctc acgcgattat
    67141 ccaaaaattg cacatctcaa ataactactt aaaaatctga gttatctaaa atttgtttac
    67201 caaaaacttc tttttccaat ctattcaggc gtttcaaaat atcaaaattc gtcatagaac
    67261 ttgtaattgc cgcttcaagg ggttctgctt gaactggtga aggtttcggt ttacgagtta
    67321 attcttcctt caaatccgca atttcctgac gaagtgactt gaccaaggca gcataggttt
    67381 catagtcctt gatgacatcg tctaaaaact cgtcaacttc tactttatta tagccacgga
    67441 cttcacgtcc aaactcttgt tcaaaaatat ctttcgctga aaaaataata cttgccatgt
    67501 ctctctccat tctcgcttgc tagtattatt atataaaaaa aggcaaacaa aaatcaaggt
    67561 caaagcttca attttcggaa aaattttcag caagttcatt taattgatca aatgttaatc
    67621 tctttataaa atagtcctct tgatttttca tcttttggta aaaataagct agttttgttt
    67681 cattttcttc atcataaaaa agataggaac tagtcgtgtt ttccagcaaa aactgctggt
    67741 aatctcttaa ctgccccttg tgttcatagc gaggataagc atatttgaca aagtctacct
    67801 gcttaaagcg actcagtttc atctgattgc cttcattcca attttcccca tgggtttcaa
    67861 aagcaaaaat ggtggccaac tggaaaccgt attcggtttt catctcctga gcaacctcta
    67921 aaacccaata ttcaaaaccc aaactccctg taaacacaag ccaagacacc ccatctgctg
    67981 ccatagcctc taaatcttta cgtatcgctt ttttaatcaa tttaagacga ggatccttat
    68041 cagaaaataa acccaaatca aaagcagaat agcctaaaac caaagctgta gccattttta
    68101 accctttctg tgcaaattat ggtataataa agtgatgtta ttgtaccaat aaggagaatt
    68161 atgatcaact atccacataa agtttcatca caaaaaagac aaacatctct ttctcaaccc
    68221 aaaaatttcg caaatcgagg aatgtctttt gaaaagatga tcaatgctac caacgactac
    68281 tatttgtctc agggcttggc tgttatacat aagaaaccaa ctcctattca aatcgtacaa
    68341 gtggactatc cacaacgaag tcgtgccaag attgttgaag cctattttcg acaagcttca
    68401 acgacggact attctggcgt ttataatgga tattacatcg actttgaagt caaggaaaca
    68461 aaacaaaaac gtgcgattcc gatgaaaaat tttcatccac atcagattca gcatatggaa
    68521 caagtccttg cccaacaagg aatctgcttt gtccttcttc acttttcttc tcagcaagaa
    68581 acctacttat tgccggcatt cgatttgatt cgcttctatc atcaagataa gggacaaaaa
    68641 tcaatgccac ttgaatatat tcgagaatat ggatatgaaa tcaaggctgg tgccttccct
    68701 caaattcctt atctcaatgt tatcaaagaa catttattag gtggtaaaac aagatgaaca
    68761 aaccaacgat tctgcgccta atcaagtatc tgagcattag cttcttaagc ttggttatcg
    68821 cagccattgt cttaggcgga ggagtttttt tctactacgt tagcaaggct cctagcctat
    68881 ccgagagtaa actagttgca acaacttcta gtaaaatcta cgacaataaa aatcaactca
    68941 ttgctgactt gggttctgaa cgccgcgtca atgcccaagc taatgatatt cccacagatt
    69001 tggttaaagc aatcgtttct atcgaagacc atcgcttctt cgaccacagg gggattgata
    69061 ccatccgtat cctgggagct ttcttgcgca atctgcaaag caattccctc caaggtggat
    69121 caactctcac tcaacagttg attaagttga cttacttttc aacttcgact tccgaccaga
    69181 ctatttctcg taaggctcag gaagcttggt tagcgattca gttagaacaa aaagcaacca
    69241 agcaagaaat cttgacctac tatataaata aggtctacat gtctaatggg aactatggaa
    69301 tgcagacagc agctcaaaac tactatggta aagacctcaa taatttaagt ttacctcagt
    69361 tagccttgct ggctggaatg cctcaggcac caaaccaata tgacccctat tcacatccag
    69421 aagcagccca agaccgccga aacttggtct tatctgaaat gaaaaatcaa ggctacatct
    69481 ctgctgaaca gtatgagaaa gcagtcaata caccaattac tgatggacta caaagtctca
    69541 aatcagcaag taattaccct gcttacatgg ataattacct caaggaagtc atcaatcaag
    69601 ttgaagaaga aacaggctat aacctgctca caactgggat ggatgtctac acaaatgtag
    69661 accaagaagc tcaaaaacat ctgtgggata tttacaatac agacgaatac gttgcctatc
    69721 cagacgatga attgcaagtc gcttctacca ttgttgatgt ttctaacggt aaagtcattg
    69781 cccagctagg agcacgccat cagtcaagta atgtttcctt cggaattaac caagcagtag
    69841 aaacaaaccg cgactgggga tcaactatga aaccgatcac agactatgct cctgccttgg
    69901 agtacggtat ctacgattca actgctacta tcgttcacga tgagccctat aactaccctg
    69961 ggacaaatac ccctgtttat aactgggata ggggctactt tggcaacatc accttgcaat
    70021 acgccctgca acaatcgcga aacgtcccag ccgtggaaac tctaaacaag gtcggactca
    70081 accgcgccaa gactttccta aatggtctcg gaatcgacta cccaagtatt cactactcaa
    70141 atgccatttc aagtaacaca accgaatcag acaaaaaata tggagcaagt agtgaaaaga
    70201 tggctgctgc ttacgctgcc tttgcaaatg gtggaactta ctataaacca atgtatatcc
    70261 ataaagtcgt ctttagtgat gggagtgaaa aagagttctc taatgtcgga actcgtgcca
    70321 tgaaggaaac gacagcctat atgatgaccg acatgatgaa aacagtcttg acttatggaa
    70381 ctggacgaaa tgcctatctt gcttggctcc ctcaggctgg taaaacagga acctctaact
    70441 atacagacga ggaaattgaa aaccacatca agacctctca atttgtagca cctgatgaac
    70501 tatttgctgg ctatacgcgt aaatattcaa tggctgtatg gacaggctat tctaaccgtc
    70561 tgacaccact tgtaggcaat ggccttacgg tcgctgccaa agtttaccgc tctatgatga
    70621 cctacctgtc tgaaggaagc aatccagagg attggaatat accagagggg ctctacagaa
    70681 atggagaatt cgtatttaaa aatggtgctc gttctacgtg gagctcacct gctccacaac
    70741 aacccccatc aactgaaagt tcaagctcat catcagatag ttcaacttca cagtctagct
    70801 caaccactcc aagcacaaat aatagtacga ctaccaatcc taacaataat acgcaacaat
    70861 caaatacaac ccctgatcaa caaaatcaga atcctcaacc agcacaacca taacatttat
    70921 cacccagatt tttctgggtg attttgtcat agcactattg tatttatgtg cttaacaaaa
    70981 gcctgagaag atgtcttctc aggctttttc tgtactataa acctcattgg aaaagataga
    71041 gactgcttcc gtaatcttgt taagaggttt tactaaatac tagtctttct tcgtttttac
    71101 tacaaagaga gcagataggg ctagactaac acttgctagg aagagggctg tgtcagattg
    71161 actctcacct gttgctggca agatttgttc aggtgtttct ggtctagcta tttcttgctt
    71221 ataatctggc gtcttacctt gatcggaaac taaagagcta agtttaaact caggtttctc
    71281 tacagttgga gcatctggtt tcgtagcaga atcttttaat tggttgagat aatctgcaaa
    71341 gtattccacc attctttccg tcaagagatg gttatccgtc gcatatttca tgcttgcctg
    71401 aacgctagct acttcctctt gattgtcttt gtctgttaat ttctgagctt tagccaaagc
    71461 tgcttcatag cctgacaagt caagaggtgt ttctgctacc tgtggacgag taaagatgag
    71521 ttccgctgca gattggtatt tatcgccacc atctccgtaa gtcttagtac ctgtaaggac
    71581 aattttctta gccttaattg tcttaccaaa atcaatgtct tttggcttat tgttatctgg
    71641 ccaatcagtt gcagtaaagg tatgctcctt gccagactca tctgtcacaa caagtttcac
    71701 atcacgcaag ttaccatttg aacctgatcc acgtggaacg taacgaagtc cagtgatttc
    71761 agttggttct ttcaagacca tggttgcagg cttgcctaca tctccaccat tccaagatgt
    71821 atgccataaa ctagataagt ttccatcaaa ggcatttgca agaccttctt gagcctgagc
    71881 aggagctgta agacttgcaa agtcatctgc taccaaagcc gttttcttct gaaccaaagc
    71941 attcttcaag gcttcaatct tggcaatctc tgcacgcgct tcttccacac tgatatcatc
    72001 atcggcctga ctaaggttaa agaccgcctc tttcaaagca tccatagact ctttggtgta
    72061 gttagtcatg gcaaccgttg gcaagtagtt cttcagagca ttttctgtca acatcttacc
    72121 tgttagggta atttcttcga tttgaagatt atccatcatg aagtcgttat aaccacggaa
    72181 gttggcattt ccaccagaat caccacgagt attacttgca ttgccagttg agtagatacc
    72241 tacccaagta tcccctgttt ctgcacctgt cacaaggaag gttgccttct tggctttctt
    72301 agaatctgtc caagtatttg gcaattcatg catttccaag ttgcttgctt gagtgccacg
    72361 acgacctgac tggaattctc ccttaccgac tacaaaagca taggtattgt ctgaacctgc
    72421 ttcgtattca aaggttacac ggtaggtctt acctgcttca aaacggaagt tttgtgggat
    72481 ggtttggtaa accaagttac gacggctcac tagtccattt gtcttgagtg accaatttcc
    72541 ttcgataaca tcatcgactt tcttaccgtt ccaaccacgt tgtgtatatg gatcgtgttt
    72601 ttcagacaag tgagtgcggt tgtcttcgac accttcgaca ccacccacta caaatgggaa
    72661 gattccttga gctacatttt caaagtcttg cttgaaggtt ccttgacctg tatcgtgctt
    72721 gtctccatac atgcttgaat tgttttcaaa ggtacgaatt tcatcaaagt aagttgcttg
    72781 atcaccagct tcacgactca atgtcagagt aacatttgag acgtccgatc ctgttgtaaa
    72841 gaaggcgtac atgttttgga agtaacttgt atcgttaact gtagcattgt tacgacgtgt
    72901 attgtgggcg taggctttta catagttgag ggcgagagac ttattggtat aagtagtcac
    72961 ttctttttca ccagtattca cagtgatact tgccttggca ttactacggt tatcgacacc
    73021 gacataaacg gcatacttgg tatttggttt caagccagtt aatttctgag tgagactaac
    73081 tttttctttg tttccttgaa tacgaagcat atcgtttgcc ccttgagact tgacaatttc
    73141 tgccttagaa gcatcgcctg aaatggtcca atgtttcaag gtaccactgt taaatccttg
    73201 gtcatagatg tgcatgcctt cactccatga catttcagga ttggtttgtt ttgaacgata
    73261 gagaacgtat ggttgatttg ctagaagatc tagggtaatt ttaccatctt ttacagttag
    73321 ttcttgctct tctgtcttac cttggtcagt tagcttgtaa aggtaaacct tgctctttgc
    73381 ccaatcgctt ggaagggtcc aagttgttgc accggcctgc gtattgaagt agtacatctt
    73441 ttccttatca gtagaaagtt tcttaccatt tgcatcccag ttccaaggag tcaagtaagc
    73501 tgaaccatct tggatgacac gtccgttgag agttactgta cgttcgcgat attgtggact
    73561 attgacatca tttgacttac gagttacaac tactttatta ttgtcagcat ctaccaattc
    73621 cactcgcatt tctggagtcc atttataggt actaccgtta tcggtcatag tcaccggtgt
    73681 accattttcc catttactta cagtgaagtg ttggaagtac ttagtcatga cgtcatgggc
    73741 aaataagttg gttacatagc cattgtagtc acttcttccc tgccagcctt caaagtcttt
    73801 catgctgtag ccacctagca gtggatagtt ggctgcacca ccataacttc tgtagtcccc
    73861 aacccaagca tctttttggt ggttacggat aaagcgggtg atggcactgt tgataccttt
    73921 attggtgtag ccaccgtagg tcaagtcagc tgcccagtga tggaaggtag agtcgtactc
    73981 accaccatgg ccccactcga tcgcaaagcg ccagccttgt ttgttaattt ctttagcaag
    74041 aacgtgggta gcccaggcac cgttatcacc tgattgacca ttaccccaaa cgtccacata
    74101 gataaagtcg agaccgtcac caagtttttt cttcaaatct tcccaacgtg ccaaacgacc
    74161 atgagctagg tcataggcag catcaatgtt gataccttga tctagccagt tccaaccata
    74221 gctatagctt ccatctggat tcttacggag aattttttca ttgaagtatt tagactcagg
    74281 ataagtttct gaagcgttaa cgtggatacc tagatgagct ccatatttct tagccttctc
    74341 aattagggtc ttgaagtctt cgacaccacc gatacgctta ccaatatcag catagttcaa
    74401 gtgaccagag tcatggcctt cgctaccata tcctttaagg aaaacacctt gcccaagacc
    74461 atctgtgtgg agattgattt tcttgatacc atccaaggtc ataaggaatg gattttgtgc
    74521 ttgagaacca aagttcatcg cgatacggta agctgtgata tccttaactt tttcccaacc
    74581 ttgaggattg ttcataatgc tacgataagc aatggcacca tcttgccaat cgactttctt
    74641 gtctgcattg gcatcttcag tgataacaac cttagcactt ggaagttcct tcgtgtattc
    74701 tgggaaaaca atgcccttat aagctttttc ccattgccat tcagagctgt ggattcctac
    74761 atagttggca tttccgactg tttctttata agctgtcaaa cgagtccagt cattcgaacc
    74821 accaccatag ctattttgag agttactcca aacaccagca gcaagcttat ctgtagaaac
    74881 aaatccatac atgtaaccct tggctaggtc tttcattgga ttggttacat cgatatgatc
    74941 atctccgctg acatgcgtat tgtttgacat ggttgcccca tcaaacttag caccagtttg
    75001 atcactagaa acagagacta aagcattgcc gaggaaacta atagaagaaa gtagttttct
    75061 ttcgtcatca atcttttgac ctggagtgac ttgattgtgg ttgacaatct tggtcacatc
    75121 aaagtgcaat tgattgtcca caacttgcaa gcgtactgtc atttccgcat tgattaagtg
    75181 agcatcatcg cgaagcttca tcaagtactc tgctgttgtc tcattgattt tcttataagt
    75241 gacttcaggg gtgattcggt ggttattgat aaagacttgg ttgaactgtt gcacctgtcc
    75301 tggcaaagta tgtccattca agctgtattc cttgacacga gggaaggctt ggtcaatcac
    75361 tgctttgagg accttagact ggatcgtatc ataagtcacc ttgctatcgt caacaaccgg
    75421 acctgtttct ttctgggcag gagtatcatc cgcttttacg ccttcttgat tgtctgtttt
    75481 aacgctgaca actgtacggt cattgccata agttcctgcc ttgagaagga tcttcttctc
    75541 atttttaaga ttatcattga ccgccccagg cagtgtcact gtgtcaaaga gattgacatc
    75601 gttattgctg gcattgagtt gtccatctga cttgagagtg atagaaagac ggtttacaga
    75661 acccggttct ggtgcaacta cacggttgcc tttataccat gtgctattac ctggagtttt
    75721 gtattcccag aaccagccat ccttgtcata accgacaaaa acattattat tggtatcttt
    75781 aaatttcagg aagacaccaa agcgtgattt gcccttttca gaatcttctt tgaaggttaa
    75841 atcaacagtt gcatttccat tggcatcaac ggtcaagccc ttcttttcaa acagggctgg
    75901 tttgcctgcg ttatcatttt gagcagttga gaatagttgg ttgtagcgga cacctttttc
    75961 ttctcggata gtgactgttc cctgttgttc ttttttctct accgtttgcc attcaggagt
    76021 taccgtctta ggtgtttcag gtttagcagc tgcaggttta tcctcttttg gtttttcttc
    76081 agccggtttt tctttttcaa gtgctaatag ttcttcttct gtcttaggtc catctgtaac
    76141 ttctggagaa ccttggtctt ctcccacctt aggcgcttca aaatcacgcc catcattctc
    76201 gtttgctgtt gcaagagcag tagctaaatc agctggtaag ttcgccgtgg aaccagactg
    76261 cacgctatct gcaagaaccg gacttgtccc aaagaatgca gctccaatca taacagaagc
    76321 aacacctaat gaaaatttcc gaatactata tttacaacgt ttttcaaata atcctttatt
    76381 cataaacttc taaatccttt cattttttag caagcgttta catttccttt ttaaaaaata
    76441 ctccttcctc tgaaagtgca atgcttaacc ctgataaata aaaaataatt gtacttttaa
    76501 tataacaaat aatagctgag aaagcaatag gaaacaagta aaattaacaa aaattttctc
    76561 tattctgaca aaaagaggct ccaacttttg ggggtcagta catttagaat ctttctttac
    76621 aaatgtaaaa aaggattcag cattcaagag aaatactaaa tcctttctta tattttgcaa
    76681 cagttatttc acatgttttg cgagatcttc ttgagccttt ttattagact cttctttttc
    76741 tttcatccat ttttcctgag ctttttggta ttcatctaca gtgactgcct tgtcttgaag
    76801 ttccaagtat ttatacaaga ctggttcact tgtaccttta tttcctgaca atgcaaatgg
    76861 tattgtaaat ggtaccatct tagacaagat tgggcgccct gtacgagatg tagttggaat
    76921 aatcaaagca ctatctgtca accaagcttg ggctgcagcg tatttatcat agcgtttagc
    76981 aacatctgta gcctcatcac cagcctcagt gaccaatttt tcgtagtcat atagacctac
    77041 ttttttagca gctacattat cttcccctga gtcaaaccct aaatatgttt tagtactttc
    77101 tcctacagaa ggtttgataa tatcaaggta ggttgatgga tcggcaaagt ctggacccca
    77161 accgacatta tctgataaat cccagtcttc gccagcagca ttttcagcaa aatatgtaat
    77221 attgtttact tcgtcttttt gtagttgttg gatatcaata atgacattat cagctcctaa
    77281 agttgcttcc aaggattgtt tcatagattg gacgcgctga acttttgtag ttgctgtctg
    77341 gtcaactggc atatccaaat gaattgggaa agtcacacct tctgcttgta aggctgattt
    77401 agctttagca aattcagcct tggctttttc tggattgtaa agaccatcct gagaatctgc
    77461 aagattaaca tccttccatt catccccata agtgaccaat ttctctttga ccatatcgcc
    77521 aaagttttta ccatctgctt gaacaaatgt tggtggaaca aagatattac gtaagatttt
    77581 gcttgctcca gtttgtccat tcaactgaga ggcataggct gtacgatcaa aaccaaaggc
    77641 aatagcctga cggaaatcct tgtttaagag agccttttta gtcgatgcct tttgttcgtc
    77701 gctggtctta gatgtatatt tataggactg acggtcaata tttgtaccaa ctagatacgt
    77761 aatagagcct tgttgagtat agacaatatt gtccttcata ctcttctcaa gctctgcgaa
    77821 acttgcactt gttggataga gacgagctgc tgtaaggcta ccatctttaa agttttctgc
    77881 aggtttgctg gtatcttgac catcccagaa tgacaattta actttgtcaa catgcacatt
    77941 gtccttatcc cagtagttcg gatttttcgc aaattcaaca gaggattttg tcacaatgga
    78001 tttcaacaaa taaggaccgt tatacaagag actacttgga tccgtcgctt tggcaaaatc
    78061 atcccctttt gagttcaaaa actcttcatt aactggcgca agcacaccca tggttgtctt
    78121 agaattccag aagctttctg gtttgttcaa agtgtactga actgtctgat catccaaagc
    78181 cttaattcct acttgtgaga aatctttgat ttcccctttt acataggcat ccaacccttt
    78241 gattgattct tgaacaaggt aaagagcatc tgatttttta tcagcagcat attttagtcc
    78301 tgttacaaag tcttgagctt tgactgccgc gtattcttca ccttcagaag tataccattt
    78361 tgcatcctta cggatagtat aagtgtaagt caatccatcc ttggatacag accaatcctc
    78421 agccatagac ggcacaaagt tcccgtagcg atcattttct agcaaaccat caaccacgtt
    78481 actggtaata tttgctgtcg cagccttagc agttgtcaaa tagttgaggt tatcagggtc
    78541 tgtctcataa atgtatgaga atgtcttctc acctttagtg cttgaacctg atccagagca
    78601 tgcagctaaa gtagtcgccg ccaataatgt cacgcccgca agggcaagta gttttgaact
    78661 tttcatcctt ttctccttta aaactttctc tccattatag actcttttca gaaagttgtc
    78721 aacagaattt tcagaatttt tgaaaattat ttttcaaata acatctttgc aaaaaatatg
    78781 aatatcgtaa ccgcccaata acgaagtcta ttgaaaaatc tccagactag agaactcacg
    78841 gatagttcct aatctggaga tttcttattt gcacttttct tgtacaactt tagtccacgg
    78901 taaataggcc tctaaaatct ctttgtttac gagagtttcc tcgtttggaa gacattctag
    78961 aagataggat agatatttct cgctatttaa t
//
LOCUS       DAVBBS010000004        64162 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVBBS010000004 DAVBBS010000000
VERSION     DAVBBS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 64162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64162
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.004"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(138..485)
                     /locus_tag="VQU32_000235"
     CDS             complement(138..485)
                     /locus_tag="VQU32_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2471764.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(596..2371)
                     /gene="spxB"
                     /locus_tag="VQU32_000236"
     CDS             complement(596..2371)
                     /gene="spxB"
                     /locus_tag="VQU32_000236"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HET2471765.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(2578..4821)
                     /locus_tag="VQU32_000237"
     CDS             complement(2578..4821)
                     /locus_tag="VQU32_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET2471766.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYIVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQSLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(4831..5202)
                     /locus_tag="VQU32_000238"
     CDS             complement(4831..5202)
                     /locus_tag="VQU32_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET2471767.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QVGEFGFACGMNMMHGKMIVE"
     gene            complement(5213..5608)
                     /locus_tag="VQU32_000239"
     CDS             complement(5213..5608)
                     /locus_tag="VQU32_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HET2471768.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTIQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      5792..5890
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5974..6765
                     /gene="thiD"
                     /locus_tag="VQU32_000240"
     CDS             5974..6765
                     /gene="thiD"
                     /locus_tag="VQU32_000240"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET2471769.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEGMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(6974..7606)
                     /locus_tag="VQU32_000241"
     CDS             complement(6974..7606)
                     /locus_tag="VQU32_000241"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET2471770.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(7599..8402)
                     /locus_tag="VQU32_000242"
     CDS             complement(7599..8402)
                     /locus_tag="VQU32_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET2471771.1"
                     /translation="MQALTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSENQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(8402..8926)
                     /gene="thiW"
                     /locus_tag="VQU32_000243"
     CDS             complement(8402..8926)
                     /gene="thiW"
                     /locus_tag="VQU32_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HET2471772.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFAERID"
     gene            complement(8931..9623)
                     /gene="tenA"
                     /locus_tag="VQU32_000244"
     CDS             complement(8931..9623)
                     /gene="tenA"
                     /locus_tag="VQU32_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HET2471773.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(9634..10284)
                     /locus_tag="VQU32_000245"
     CDS             complement(9634..10284)
                     /locus_tag="VQU32_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HET2471774.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAIFPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQKLHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAYGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(10286..11671)
                     /locus_tag="VQU32_000246"
     CDS             complement(10286..11671)
                     /locus_tag="VQU32_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2471775.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIVERVQTVGILLQNPNHQFTMESLFEELVFTMENI
                     GYHLQEIDSKIAEVVQQCRCKDILHRLIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELVYDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGSITWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(11672..12232)
                     /locus_tag="VQU32_000247"
     CDS             complement(11672..12232)
                     /locus_tag="VQU32_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2471776.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGFVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     regulatory      complement(12494..12582)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(12791..13420)
                     /locus_tag="VQU32_000248"
     CDS             complement(12791..13420)
                     /locus_tag="VQU32_000248"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET2471777.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFGTQSKDDAGGTIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(13422..14204)
                     /gene="thiM"
                     /locus_tag="VQU32_000249"
     CDS             complement(13422..14204)
                     /gene="thiM"
                     /locus_tag="VQU32_000249"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET2471778.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVMIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            complement(14447..15115)
                     /locus_tag="VQU32_000250"
     CDS             complement(14447..15115)
                     /locus_tag="VQU32_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HET2471779.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVDKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(15330..15418)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15672..16808)
                     /gene="lctO"
                     /locus_tag="VQU32_000251"
     CDS             complement(15672..16808)
                     /gene="lctO"
                     /locus_tag="VQU32_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HET2471780.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(17433..18923)
                     /gene="lysS"
                     /locus_tag="VQU32_000252"
     CDS             complement(17433..18923)
                     /gene="lysS"
                     /locus_tag="VQU32_000252"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET2471781.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLITKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKTIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(19051..19197)
                     /locus_tag="VQU32_000253"
     CDS             complement(19051..19197)
                     /locus_tag="VQU32_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HET2471782.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAGDTF
                     TSFQ"
     gene            19423..20091
                     /locus_tag="VQU32_000254"
     CDS             19423..20091
                     /locus_tag="VQU32_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2471783.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRILV
                     LAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFYYVVLPQATAVALPSFSANVIFLIKETSVFSAV
                     ALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPST
                     LSRK"
     gene            20051..20728
                     /locus_tag="VQU32_000255"
     CDS             20051..20728
                     /locus_tag="VQU32_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2471784.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIEISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLDFTRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GAAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            20729..21486
                     /locus_tag="VQU32_000256"
                     /pseudo
     CDS             20729..21486
                     /locus_tag="VQU32_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            21498..22359
                     /locus_tag="VQU32_000257"
                     /pseudo
     CDS             21498..22359
                     /locus_tag="VQU32_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(22439..23086)
                     /locus_tag="VQU32_000258"
     CDS             complement(22439..23086)
                     /locus_tag="VQU32_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2471785.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(23083..23781)
                     /locus_tag="VQU32_000259"
     CDS             complement(23083..23781)
                     /locus_tag="VQU32_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471786.1"
                     /translation="MGKLSSHMWRLNKIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDVGGDLFLQLLFLVFLFQ
                     SIFYFTRQKRRFIE"
     gene            complement(23791..24543)
                     /locus_tag="VQU32_000260"
     CDS             complement(23791..24543)
                     /locus_tag="VQU32_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471787.1"
                     /translation="MKQFVQFYKKDFLSVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLNNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            complement(24543..25307)
                     /locus_tag="VQU32_000261"
     CDS             complement(24543..25307)
                     /locus_tag="VQU32_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471788.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(25403..25806)
                     /locus_tag="VQU32_000262"
                     /pseudo
     CDS             complement(25403..25806)
                     /locus_tag="VQU32_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25986..26618)
                     /locus_tag="VQU32_000263"
     CDS             complement(25986..26618)
                     /locus_tag="VQU32_000263"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET2471789.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYRELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(26652..27353)
                     /gene="pyrF"
                     /locus_tag="VQU32_000264"
     CDS             complement(26652..27353)
                     /gene="pyrF"
                     /locus_tag="VQU32_000264"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET2471790.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQNWN"
     gene            27674..27766
                     /locus_tag="VQU32_000265"
                     /pseudo
     CDS             27674..27766
                     /locus_tag="VQU32_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(28088..28801)
                     /locus_tag="VQU32_000266"
     CDS             complement(28088..28801)
                     /locus_tag="VQU32_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2471791.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEGFSWNKTLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(28794..29489)
                     /locus_tag="VQU32_000267"
     CDS             complement(28794..29489)
                     /locus_tag="VQU32_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2471792.1"
                     /translation="MTGQFLADKGQSIILGQKSQNLTSGELKRIGLVSDTSGFYEKMS
                     LYNNLLFYSKFYNISKSRVDNLLKRVGLYDSRKMVAGKLSTGMRQRMLLARALINKPA
                     VLFLDEPTSGLDPTTSRTIHELILELKTAGTTIFLTTHDMNEATLLCDYVALLNKGKL
                     VEQGAPSELIQRYNKDKKIKVTDYNGNQITFDFTSLEQVSQADLENIFSIHSCEPTLE
                     DIFITLTGGKLNA"
     gene            complement(29829..31161)
                     /locus_tag="VQU32_000268"
                     /pseudo
     CDS             complement(29829..31161)
                     /locus_tag="VQU32_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812020.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
     gene            complement(31145..31943)
                     /locus_tag="VQU32_000269"
                     /pseudo
     CDS             complement(31145..31943)
                     /locus_tag="VQU32_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824573.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50 family protein"
     gene            complement(32733..33932)
                     /gene="divIB"
                     /locus_tag="VQU32_000270"
     CDS             complement(32733..33932)
                     /gene="divIB"
                     /locus_tag="VQU32_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HET2471793.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLSKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            complement(33942..35000)
                     /locus_tag="VQU32_000271"
     CDS             complement(33942..35000)
                     /locus_tag="VQU32_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HET2471794.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVCWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VAKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQLENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(35002..36354)
                     /locus_tag="VQU32_000272"
     CDS             complement(35002..36354)
                     /locus_tag="VQU32_000272"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET2471795.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(36458..37099)
                     /locus_tag="VQU32_000273"
     CDS             complement(36458..37099)
                     /locus_tag="VQU32_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2471796.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTFL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNCVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTTSLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(37101..39122)
                     /locus_tag="VQU32_000274"
     CDS             complement(37101..39122)
                     /locus_tag="VQU32_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2471797.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLMSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            complement(39193..39492)
                     /locus_tag="VQU32_000275"
     CDS             complement(39193..39492)
                     /locus_tag="VQU32_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET2471798.1"
                     /translation="MKTKKHRLLALALISSFTLLGAASAAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSLVSRWNSHSDKGEASAGKTSYAWIWTKWGEQVAFYCDYD"
     gene            complement(39858..40112)
                     /locus_tag="VQU32_000276"
     CDS             complement(39858..40112)
                     /locus_tag="VQU32_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HET2471799.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            complement(40132..41973)
                     /gene="typA"
                     /locus_tag="VQU32_000277"
     CDS             complement(40132..41973)
                     /gene="typA"
                     /locus_tag="VQU32_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET2471800.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            42135..42860
                     /locus_tag="VQU32_000278"
     CDS             42135..42860
                     /locus_tag="VQU32_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HET2471801.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVQVNELLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
     gene            complement(42900..43127)
                     /locus_tag="VQU32_000279"
     CDS             complement(42900..43127)
                     /locus_tag="VQU32_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471802.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(43216..43596)
                     /locus_tag="VQU32_000280"
     CDS             complement(43216..43596)
                     /locus_tag="VQU32_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2471803.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            complement(43596..43865)
                     /locus_tag="VQU32_000281"
     CDS             complement(43596..43865)
                     /locus_tag="VQU32_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HET2471804.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            43929..44837
                     /locus_tag="VQU32_000282"
     CDS             43929..44837
                     /locus_tag="VQU32_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET2471805.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            complement(44891..45646)
                     /locus_tag="VQU32_000283"
     CDS             complement(44891..45646)
                     /locus_tag="VQU32_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET2471806.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(45667..46596)
                     /locus_tag="VQU32_000284"
     CDS             complement(45667..46596)
                     /locus_tag="VQU32_000284"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HET2471807.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            complement(46611..47234)
                     /locus_tag="VQU32_000285"
     CDS             complement(46611..47234)
                     /locus_tag="VQU32_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HET2471808.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            complement(47227..48465)
                     /gene="hflX"
                     /locus_tag="VQU32_000286"
     CDS             complement(47227..48465)
                     /gene="hflX"
                     /locus_tag="VQU32_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET2471809.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            complement(48458..49342)
                     /gene="miaA"
                     /locus_tag="VQU32_000287"
     CDS             complement(48458..49342)
                     /gene="miaA"
                     /locus_tag="VQU32_000287"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET2471810.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKCFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            49463..49633
                     /locus_tag="VQU32_000288"
     CDS             49463..49633
                     /locus_tag="VQU32_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HET2471811.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            complement(49681..50520)
                     /locus_tag="VQU32_000289"
     CDS             complement(49681..50520)
                     /locus_tag="VQU32_000289"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET2471812.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPIITLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(50617..51576)
                     /locus_tag="VQU32_000290"
     CDS             complement(50617..51576)
                     /locus_tag="VQU32_000290"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HET2471813.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENTFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            complement(51703..52701)
                     /locus_tag="VQU32_000291"
     CDS             complement(51703..52701)
                     /locus_tag="VQU32_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2471814.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DLHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQAATTQTDTTSSTASGWVKQDGS
                     WYYFDGNGNLVKNAWQGNYYLKADGKMAQSEWIYDSSYQAWYYLKSDGSYAKNAWQGA
                     YYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTA
                     SSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDG"
     gene            complement(52751..53368)
                     /locus_tag="VQU32_000292"
     CDS             complement(52751..53368)
                     /locus_tag="VQU32_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2471815.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            complement(53369..55090)
                     /gene="pabB"
                     /locus_tag="VQU32_000293"
     CDS             complement(53369..55090)
                     /gene="pabB"
                     /locus_tag="VQU32_000293"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HET2471816.1"
                     /translation="MHRKTVIDFRTLGERYTFAQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            complement(55159..60846)
                     /locus_tag="VQU32_000294"
     CDS             complement(55159..60846)
                     /locus_tag="VQU32_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2471817.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEETPKEEAPRTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKSEEKVAVKPESQPSDKPAEES
                     KVEPPVEQAKVPEQPVQPTQAEQPSTPKESSQEDNPKEDRGAEETPKQEESTPDTKAE
                     ETVEPKEETKTAKGTQEEDYVGEALVPEQLPEYKVTEGTLVEESTTELDYKTETTEDP
                     TKYTDEETVLRNGEKGSQVTKTTYKTIGGVKTDQVLSTSTEVTKEPVNQQVIRGTKPI
                     EGTTVETREEIIPFETKEQEDDTLKRGTRQVTQEGVNDKKQITETYKTIRGEKTSDAP
                     TITETVIEKPQDKIIKNGTKELEKPTLEWAKTDKDVLKKSATASYTLTKPAGVEIKSI
                     KVALKDNTGTVVKEVTVEENNLQATLDNLKYYQGYTLSTTMVYDRGNGEETEILEDKE
                     VQLDLKKVEIKDIKETRLMKVDENGNETDSSLLETVPENLTSYYLKITTNHNKVTRLA
                     ITNIEEVTEEETSLFKVTASAPDLIQRDSNNSLSQTYTYYIEKPKASQNNVYYNFKDL
                     VDAMQKNPNGEFKLGSDLNATNVPTPSKSYVTGKFTGHLTSVDGKHFSIHNTARPLFD
                     RVEGGSIKNINLINVNINMPWANQLASLAQTVKNATVENIKVTGTIVGNNNVAGILDK
                     LDDAGIMTNVAFIGKVQSVGDKGWNLAGIASEIYRGNLNKAYADVELKGLNTRAGAIV
                     GHLAHDFNPRDVGAWSNVRNSVAKGTIDVARGDVGGLIGKDWPWGTIDNNISLVNVKR
                     GDIFFGSNDMDDEFVSNWIKPNNATVIGVSSGDKTHKQSSKIKEISQVEADKKIKDMG
                     ITADTFEVTEHLVDKLNRRILRDEEYKTIQDYNANRELVYRNVEKLQPFYNKEWIVNQ
                     GNKLTTDSPLMNKEVLSVTAMKGNDFITDLTDADHIMVHYADKTKDIFTISPKDSQVK
                     QVKEYSVAELGEVVYTPNMVVKDRADLISAIEGILSPIELQSDPIYQRLGRTGDNRVN
                     AIKNLYLEESFKEVKDHLNTFVTKLVENEDHQLNTDEAAKRALIKKIEDNKAAVLLGL
                     TYLNRYYGVKFDNFNIKELMLFKPDFYGKNVSVLDRLIEIGSKEDYIKGSRTQESFRE
                     VVAKNTLSGNLHDFLTYNMKLFTNETDINVWFKKAIENNAYVVEQPSTNPAFANKKYR
                     LYEGLNNGQHGRMILSLLNLKDAHLFMISTYNTISFSSFEKYGKDTEEKRESFKSEIN
                     KRAKEQVNYLDFWSRLATDNVRDKLLKSQNVVPTPVWDNHGAPGGWPDRFGHRKDKPD
                     YAPIREFFGRIDKYHDYKYGYGAYAYIFADPQPMDAVYFVMSDLISEYGTSAFTHETT
                     HVNDRMAYLGGHRHRQGTDLEAFAQGMLQTPAEHGHQGEYGALGLNMAFERSNDGNQW
                     YNPDPTKLQTRDQIDHYMKNYNEAMMMLDYAEAEAVLPKVKGDNSKWFKKIDRETRRP
                     MDRNKLSGPHQWDKVRDLIDAEKTTNLETIDDLVNNNFMTIHGNPGNKVFHPEDFGTA
                     YVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGYYGYEHGFIDYVSSKHQGAANKEN
                     NGLLGDDFIIKKVSNNQFSNLEDWKKQWYGEVLTKSKKGFAPIEINGTQISTYEQLKT
                     MFDEAVTKDLTELNDSSIKDKYRNTVVLKSKVFKALLKNTDGFSGDLFTAPQA"
     gene            complement(60868..61431)
                     /locus_tag="VQU32_000295"
     CDS             complement(60868..61431)
                     /locus_tag="VQU32_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HET2471818.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGVLFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            complement(61547..63238)
                     /locus_tag="VQU32_000296"
     CDS             complement(61547..63238)
                     /locus_tag="VQU32_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2471819.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            complement(63235..63972)
                     /locus_tag="VQU32_000297"
     CDS             complement(63235..63972)
                     /locus_tag="VQU32_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2471820.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
BASE COUNT        19313 a        13918 c        11319 g        19612 t
ORIGIN      
        1 atgctttttg aaagcagatg agtattatat gcaatttctt tatataaaaa gaccggattg
       61 ctccgatctt tcaatagttc atattctcaa tttctatttt aaaaatagct aaggttaacg
      121 tcaaatgact acgcgaccta tttcatacga taaaaatcaa gcactagacc agcaggtcct
      181 tgaactaata aggactctgt tccccaatcg gttacagttg gtccgtgtaa aacctttata
      241 ccaagctcat tcaaccgttt gtagttctgg tctacatcct caacctcgat atgaataatg
      301 attcctgact gaaagttttc caaaggaacc aaatgatttt gtgacaacat aaggcagtga
      361 ctaccaatcg taaactgagc aaaaccatca tcagcataat ctgccttttt atccaaaata
      421 tgctccaagt cagcacagac ttggggaaca tttgaaacga taatatctaa ttgatttaaa
      481 ttcatttact ctcctccata aaaagaccga attgctccga tcttttaaag ttctgctcta
      541 tgaaaatcaa agaataaagt ctacaagttt catatttgat tttcggcgag aggaattatt
      601 taattgcgcg tgattgcaat ccttcttctt ccaagaagag acggaatggt acgagttctt
      661 ctgcttcgta tttttccttg aaggctttga tagcttcttc tgagtgaagt tttggatcca
      721 attcaagtac ttctactgga agtggacggt gttgagtgat gcgagcatcg atgacaacag
      781 ttttaccttc tttgttcaat ttaacagctt ctgcaacaac tgcatcgatg tcttcgatac
      841 ggtcaactgt gaatccaaca gctccttgag cttccgcaat tttagcgtag tcagcgtttg
      901 tgaagtctac accaaacaag tgtttgtttg tatcttcgta tttgttcttg atgaagccgt
      961 actcagcatt tgagaagaca aggttgataa ctggaaggtc gtattgaacg tttgtgataa
     1021 cgtctgggta gcacatgttg aatgctccat cacccatgat gttccatact tggcgatctg
     1081 gattgtcttt cttagcagcg ataccaccag gaagggcaat acccattgtc gcaaagagcg
     1141 gagatgtacg ccacatattc ttaggtgtca tgtgaaggtg acgagtagat gtttgagtag
     1201 tgttacctac gtcgattgag tagatagcgt cttgatcagc atgtttgttg attgcattgt
     1261 aaacttgata caattgcaat tcaccctcag ttttaccttc gagtttgttc atgtaatcac
     1321 gccagttttg gttgttctta acgtttgcac gccaccatgg agttgattca actgggttta
     1381 ctttgtcaag gatagcttta gctgcttgac cagcatcacc aaggattgaa gcgtcaaggg
     1441 catgacgttt accaagtttg taagggtcga tatcgacttg gatgaatttt tcagtgttct
     1501 tgaatgcttc gtaaacttca gcaaatggga agtttgaacc aaggaaaaga actgtgtctg
     1561 cttcaaagac cacttcgttg gctggtttcc aaccaacacg gtaagcagaa cctgtcaaac
     1621 cttcatagtt ccattcgaaa gcttcaaagt ttttaccagt tgtgatgatt ggtgctttga
     1681 ttttacgtga caattcagta atcacttcac cagctttaac accaccaaat ccagcataga
     1741 taactgggcg ttcagcattg ttcaagattt caacagcttt gtcgatttca acttcgttca
     1801 aagcaggagc gatgaatgag cgttcgtatg aacctgaacc gtagtatgag ttttcgtcga
     1861 tttcttggaa accgaagttt actggaattt caacaacagc tggacctttt ttagaaattg
     1921 cagcacggca ggcttcgtca attacttttg gcaattgctc agcgtaagct acacgtttgt
     1981 tgtaaacagc gataccgttg tacattgggt tttggttaag ctcttggaaa gcatccatgt
     2041 tcaattcgtt aactggacgt gatccaagga tcgctaggaa tggagtgtta tccatagctg
     2101 catcgtaaac accgttaatc aagtgagtcg cacctggacc acctgaacca actgcaaccc
     2161 cgattgagcc gccgaattta gcttgcataa ccgctgcaag agcacctgtc tcttcgtggc
     2221 gaacttgtaa gaagcggata tctttgtctt cagccaaagc gtccatcaat gagctgagtg
     2281 ttcctgatgg gataccgtag attgtatcta cgccccatgt tttcaatacg ttaagcattg
     2341 ctgcagatgc agtaattttc ccttgagtca taatgataac tctccttcaa tttttttaga
     2401 tttggagaat acgattacat agaattggaa acgttctcca aatttttact attccactgt
     2461 atcatattta tgctgacttt tctaaaaatc tgctcaaaac tctctattct ctattctaat
     2521 acagttttga aagttctgtc atttctgttt tataacaaag aaatctagtc attactttta
     2581 gtctatttta ctaaaattta acagaaggga actggtcaga acagatacag aactaaaggc
     2641 catggctaga cctgccaatt ctgggttgag agccagtcca acacctgaaa agactcctgc
     2701 tgcaatcgga attccgacaa cattgtagat aaaagcccag aaaagattga gtagaattcg
     2761 atgaaaggtt ttcttactca tatcaaaggc acgaaccact cctaaaagat tattggttgt
     2821 caacaccaaa tctgctgact cgatggcgat atctgttcca gctcccatag caatccccac
     2881 atctgctaca ctaagggcag gagcgtcatt gataccgtcc ccaacaaagg ctactttccc
     2941 tgactgttgc agtttatgga tttcatgggc tttttcttct ggcaagacgc ctgcaatgac
     3001 ctcttcaatt ccgatttgat ctgcaatagc acgcgccaca ccagcattgt ctcctgtcag
     3061 catgactgtt cggagaccac gtttttttag ctgactgatg gctagcttag cattttcctt
     3121 aggaatatct tgcaaagcaa gcaagccttt gatttcattg tcaacagcta agaacacaac
     3181 tgtcttagct tctttttcta gttcttctag tttatcttga taagtattag aaatatccat
     3241 accatccagc attttagcat ttccaagtaa aacttgtttt ccattgattc gccctgaaac
     3301 acctttcccg tgcaaggact gaaaattttc aacagtttga aactcaagtc cagcttcact
     3361 cgctcgctta acgatagcct cagccagtgg gtgttgagaa gcatcttcca aggaggctgc
     3421 caatccaaac acttctactt cgtcgccgat gatatctgtt accacaggtt tcccttccgt
     3481 caaagtcccg gtcttatcaa agacaagagt ttgaactttc tggatttcct gtaagacagt
     3541 tccatttttg aggagaaccc ccatcttggc actacgtcct gtccccacca taagggctgt
     3601 cggtgttgca agtcccaagg cacaaggaca ggcgataatc aaaaccgcca ctccgtagag
     3661 aagagaggac acaaagctag ctccaagcac gaccacacta tccctgagca agacgaacca
     3721 aacccaaaag gtcatgattc ctaaaatgac aactactggg acaaaaatcc ctgaaatctt
     3781 atccgtcaag tcctgaatcg gcgcacgact tgtctgagct ttcttcacaa aatccacaat
     3841 ctgagccaaa acagtctctg agccaacttt ttctgctcta aagacaagcg ttccactatg
     3901 attgatggtt gagccaatga cagtatctcc aactgtcttg tccacaggca gactctcacc
     3961 tgtcaccatg gattcgtcaa tactagagac accttctact acgacaccat caacagcaat
     4021 cttttcaccg ggacgcactc gaatcaggtc gcctaccttg acttgttcca aaggaacttg
     4081 gacataacta tcatcactca agacttctgc ggttttagct tgcaagtcca gtaatttctc
     4141 cacagcttgg gacgtatttt ttctcatttt ttcctcaaaa actgctccca aaagaacgaa
     4201 aaagaggata aatccagcac tttcgaagta aacagggaga ccagcaaaga gagcaactag
     4261 gctatagaaa taagccacta gagttcccag cgcaaccaag gtatccatgt tggcattgtg
     4321 ctttttaaaa ctggcccaag cactctggat atatggctta cctgcaacta acataatagg
     4381 cgttgttgct agaaaggttc cccaatgcat gacttgatga ctaatgctac ctgtcaacat
     4441 cccaatcatg agaatcacaa gaggcacagt aaagatacta gtaatccaaa aacgttgcag
     4501 gagagataga gattttcgag tcttctcaac gactatatag cttccctttt gcatcttcat
     4561 gccacaagaa aattcatgtc gccctaattc ttgaggtgta aaacgaatga ctttctcctc
     4621 atctacgccg attggttcca agataccttc ttcttcaaac agaatttcct tataacagtt
     4681 tgaaggagta gcacgatgaa aggtaatctc agctggaatt cccttttgaa gctggatatg
     4741 ggctggatga tagccttttt cagctcggat acggattttt tgaatgccat tttctaagct
     4801 tgctttcaca atttctgtca tagtctccac ctactctaca atcatcttgc cgtgcatcat
     4861 gttcatacca caagcaaagc caaactctcc aacctgctca ggcgtgattt ccactacata
     4921 ctcttccccc attggcaggt tcgcatgtac accaaaatct ggaaaaacaa tttgatccag
     4981 acatggtgaa ggatccttgc ggtcaaagac aatgcgtgct ggcactgatt tcttgaggac
     5041 aatcaactca ggagtatagc ctcccatgac ttccactcga atctcttggt atccgttttt
     5101 ttgctgggct ttttgtccag atttttcagg ctttttgaaa aaccaaaaca agataaacgc
     5161 gataagggca atacaaataa tggttacaat actatttaac atgacgtctc ctttacatac
     5221 aattacatct tacttctgtt acagcacttg atttcttctc tgaaatcaca gcttccaagt
     5281 cttccaagtc agtctgagta aattcacatt ctacaatcaa gtcagccaac aaattcctaa
     5341 tcctacggga acaaaccttg tctttgatat cttggacaag taaatcccga ctttggtcta
     5401 gagttaaaag ggctgaataa acaaaggact tgccttcttt tttccgagtc agacactctt
     5461 tctcaaccaa acgagccaaa agagtttgaa tggtcgactt ggaccagtcg aaccgctctg
     5521 ccaaaaccct aatcaaatct gtactggtct gctccccctg catccaaata atcttcatga
     5581 cctgccattc tgcatctgaa atctgcatta ccatacctcc aaaatctaca tttgtcaatt
     5641 acactcatca gtatactctt aaaatctaca tttgtcaatt atagaaataa tattttcttc
     5701 gaaaaataga attttaatca tttgaaaaac gatttgcagt caaatattac tatataaaca
     5761 ataaaaatat gctatactaa agaaaaaaga aaacaaccac taggggtgcg taaagctgag
     5821 attaacgact gttagatccc tctgactcaa tctaggtaat gctagctgat ggaagtggaa
     5881 atgataatgg ggactagcag tcttctattg cctttctaaa acagactagc ttgttcttaa
     5941 gaatacaaac ttcagttggt tgggaggttt tagatgactt atttacccgt tgctttgacc
     6001 attgcaggga ctgaccctag tggtggtgct ggcattatgg cagatttaaa gtcattccaa
     6061 gcgagagatg tctatggaat ggctgttgta accagtcttg tcgctcaaaa taccagaggt
     6121 gttcagctaa tcgagcacgt ttctcctcaa atgttgaaag cccaattgga gagtgtcttt
     6181 tctgatattc cacctcaggc tgtaaaaact ggaatgttgg ctactactga aatcatggaa
     6241 atcatccaac cctatcttaa aaaactggat tgtccctatg tccttgatcc tgttatggtt
     6301 gctacaagtg gagatgcctt gattgactca agtgctagag actatctcaa aacaaactta
     6361 ctacctctag caactattat tacgccaaat cttcctgaag cagaagagat tgttggtttt
     6421 tcaatccatg accccgaagg catgcagcgt gctggtcgcc tgattttaaa agaatttggt
     6481 cctcagtctg tggttatcaa aggcggacat ctcaaaggtg gtgctaaaga tttcctcttt
     6541 accaagaatg aacaatttgt ctgggaaagc ccacgaattc aaacctgtca cacccatggt
     6601 actggatgta cctttgctgc agtgattact gctgaactag ccaagggcaa gagtctttac
     6661 caggcagttg ataaggccaa ggcctttatc acaaaagcta ttcaagatgc ccctcaactc
     6721 ggtcatggtt ctggtccagt caaccataca acttttaaag attaagaaaa aaactctcta
     6781 gttcccactt taagggaatt agagagtttt tatactcttc gaaaatctct tcaaattacg
     6841 tcagcttcca tctgcaacct caaaacactg ttttgagctg acttcgtcag tcttatctaa
     6901 aacctcaagg cagtactttg agcaacctgc gactagcttt ctagtttact ctttgatttt
     6961 cattgagtat taattaggaa agaatgtcat gcaactttgt taaaaaggct tgcgtttttg
     7021 cctcaatatc ttctgcttgc atcaaatcac gtacaacagc tacaccagct atgccagtgc
     7081 ccataagctg atcaatattc tccgaagtca agcctccaat agcaactact ggaatggcaa
     7141 ccgtttggca aattgttttc aaggtcgata tcagagtaat gggcgcattt tccttggtgg
     7201 tggttgggaa aatggctcct gtacccaagt aatctgcacc tgatttctcc gcttccagag
     7261 ctcttttaac cgttttagcg gtgacaccga ggattttttc aggacccaag actttgcgag
     7321 ctaccgaaac tggtaattca tcatctccga tatgcagacc tgctgcatca accgcaagac
     7381 aaacatccaa ccgatcatcg attatcaagg ggacctgata ggcatctgtt atttccttga
     7441 cttgttttgc cagttgataa tattgattgg ttgtgagatt tttttctcgc aattggacta
     7501 tggtaacccc tgaacggcag gccgtctcaa cttttgcaag aaagctttcc acggaatctt
     7561 gatagcgatt ggttaccaga tatagtctaa gtgcttctct attcataaac ctctcctttg
     7621 atggtatcta gccaattttc atctcttctt aggagcgaaa gctgattgag tacttggtaa
     7681 cgaaattctt ccaatcccat tccttgaaca actattttct cagcagcgat attgagataa
     7741 gagactgcta agcaagaagc ttcaaaacca gtctttcctt ggctgagaaa aacggctgtt
     7801 aaggctccaa ccaagtctcc tgtccctgtt atccagtcta attcagtaca gccattctcc
     7861 agtacagcaa cttgattctc cgaaacaata aggtccttgg ggccagtaac taagaaagac
     7921 ataccaggat aggtctgaca ccagtctttc aagacttgaa gcaaatcctc cgtttcttga
     7981 tctttagcac tcgcatcgac cccaacgccg tggtgcttta atccaacaag acttcgaatt
     8041 tctgacatgt ttcctttaag gaccgtaggt ctatagtcta aaaggtcttt aactaagctc
     8101 ttacgaatgg atgaagtcgt tacgccaacc gcatctacta ccatcgggag agaagattgg
     8161 tttgcatacg aagctgccat gcggattgct ttttccttct cagctgacaa atgccccaaa
     8221 ttgatgaaga gagcctgact ttgcttagta aaatcaagaa cttcacgggg atcatctgcc
     8281 atgacaggtt tgcatcccag agccaaaatc ccatttgcca gcatctcaca agaaatctca
     8341 ttggtcatac agtgaatgag ggaactagag cctataggaa aaggatttgt caatgcctgc
     8401 atcagtctat cctttcagca aagaaatatc cttgcacttt tttaaagaat tcctgcttga
     8461 ttaaaaatct aaatgcaata aaggaaatcg ctgtaccaat caaggttgct ccgaaaaatc
     8521 gaggagtgta gataaaccaa ctaagcttag cagccgatcc tgtaaagagc accataacag
     8581 gataggaaac aatagaacca ataatacctg ttcccacaat ttctcccaag gcagaaaagt
     8641 aaaattttcg accgtactta taaaagagac ctgctagaag ggctccaaaa gtcgctcctg
     8701 tgagagctaa aggcggaatc ccttgagtcg tcatacggat aaaggctgtc actgtagcca
     8761 tagccaaggc ataaacaggt cccatcatga ttcccgctag aatattgact acactggaca
     8821 tcggtgccat tccctcaatc cgaaagatag gtgtaaggac tacatcaagg gcaatcatca
     8881 tagataaaat ggtcaatttg tgaacttgta gttggtgctt tctcatgttt ctattcttct
     8941 cctttttcta aagactgtaa atcgctcttc catgtctggt gttggtaagc catctcccaa
     9001 aacttggctt ccatatgaac actgatgtgg aaggcatcta gcattttttg cttatctgtc
     9061 tcatcacttt ctcgataaag ctgattgacc agtgctccct cctctctgat ctgttgctct
     9121 aactcatccg taatataagt ttcaatccat tgttgataga gaggatttgg tgatggttta
     9181 agattaagtg atttgcctat atcatggtat aaccaaggac aaggaagcaa gcttgcaaaa
     9241 gctatggcta agttcggttc tgcaaattgc ctataaatat gagaaatgta atgataacag
     9301 gttggagcga ttggatgttg ctccatttcc tggtcgttga tttccaattc cttgaaaaat
     9361 tgttggcgaa taaataactc accctccact aaaccctgag cattttgttt caagagtctt
     9421 ttcatctctt ggtttgaagt cttatcagcc aaaagatgat agatttctga gaaagccttc
     9481 agatagtagg catcctgaat caggtaatag cggaaaatgg caggttctaa attcccctct
     9541 tgtaattgta aaataaaggg atgatgaaag gaagcctgcc aagctttctt ggataattcc
     9601 atcgcaatat ctgtaaattc cataataact cctttataaa aatagactgg tttgaagcaa
     9661 taaaaagaaa agcaggtaga ttaattttgt ttttttagga atataaaaag tccgatagct
     9721 attcttcaac tgtgcatgtt cgtcatatcc gtaagcagat agagctctca ggtaaagatg
     9781 gcgccaccta aagactgtca tcagaacctt actgtaaatc aagggcgacc aaaaatgtag
     9841 tttttgacca cgtaataggc aagcttcttt gagggacttg atttcttgct gaatgagagg
     9901 aaaagaattg aataccacaa tcaaggcata ggaccaagag cgtgatagcc ccttttgagc
     9961 caagtacaag agaagctctt ttagtgaaat agaggaaaca aagacaaggc cgatacaaac
    10021 tgtcacaaag gccctcgttc caagcatgac tgcctgtgaa gcatctccgt gtaactgaac
    10081 tgcccagtag ttggcaaaag atgggaaaat ggcaagtatg atcatccaag ctaacatttt
    10141 aaatcgacgg taatagagca taaagagaat acaaaatgcg actaccgaaa gattcagagc
    10201 aatcgaagga atgaaagatg tttccaagga taaaatcagc aagaagagac tgataatcgg
    10261 tgtctgggtt gctactttga ccatactatc tcacctcccc ttgggtattg ctactctgag
    10321 atgtaagtgg tttggtaatg gtcacttctt tcacatgccg aagaccctga ctagtcatct
    10381 caatccaata atcaaccaca gaaatcaaag ggtctaaacg atgactaatg agcagaaaac
    10441 ttcttccttg attcctctcc tccacaatcc acttgcaaaa ataatggcag gctctatcat
    10501 ccaaacctgc aaaaggttca tctagcaaga tcacggaagc cttactggtc aagatggtca
    10561 ggagctgaag aattttttgc tgaccaccac ttaattgata gggactctta tcgactgcct
    10621 gctccaaatc aaaatatcgt aaagcttgaa aaatccgctg atttctttca gaatcaggtc
    10681 catctaattg aagctcctct cgcagactga ctcggataaa ctgcttctca gcttcctgaa
    10741 caacaccagt cagatcacga tacaaactct ttttcttttt caggaccgaa cccttccaag
    10801 taatgctccc cttatacttt tgaaattgaa gaatagaccg aaagagggtt gatttcccga
    10861 caccattgtc acccaggata caggaaatcc cttgatagaa tgtgaaatca gcaattgaaa
    10921 agagggggcg attaccaagc tcaccagtca cacggttcat atggaatagt tccgggctag
    10981 aagcaacttc ctttgaagca acctgtgtca tctcatagga agggatttga aacacttccc
    11041 ttagttttcc gtctcttagc tccaccatat ggtcgatata ggctttatag tcagataaat
    11101 catggtcgca caaaataact gtcttcccat catagaccaa ctcttttaga atctccaata
    11161 tctcgattct gctcttgcgg tcaatggaag cgaagggctc atccaagaga tagaccctag
    11221 gattcatggc aaagaggaca gccagcgctg ctttttgctt ttccccacct gataagtgat
    11281 ggatgagacg gtgcaagatg tccttgcaac gacattgctg gacaacctct gctattttag
    11341 aatcaatttc ctgaaggtga tagccgatat tttccatggt aaaaaccaac tcctcaaaca
    11401 agctctccat ggtaaattga tgattaggat tttgcaagag aataccaacc gtctggacac
    11461 gttcgacgat agaaagctga ctgacctcgc tcccatctat caggacttga ccgctatagg
    11521 gaagagaact aacttgggca atcatttgaa agaggctgga ttttccagac ccactactcc
    11581 caactaacaa ggtaaaggct tgcgcatgaa aagtaaaatc aaacggctca gagaagattg
    11641 gggactgaat cgctcgtagt tccagaccca tctatgcttt tcctccagtt gcaaactgat
    11701 gatagagttt gacaatggca cgaaccaaga tggtacagaa gaaataaaca gaaataaaac
    11761 gtaccacaag caaggaaagg acaaacggaa gggaaaaggc gtagtaacct aacttaatgt
    11821 attcatagac aaagctaaca agcgtaatcc caatactatt agcagttaga gagagccaac
    11881 tttcatagcg attcttagtt acgataaaac caaattcact tcccaaacct tgaacaaagc
    11941 cagacaaaag agctcctaga ccaaattggc taccataaag gacttcagca agcgcagcta
    12001 gcacttctcc aatcgttgca cttccgactc tcggaacaaa gatggcagca atgggcgcag
    12061 ccatacacca gagaccgaag aggatttcat tggcaaaggc ctgcaaacca agaggtgtta
    12121 agagtagact gagaatatta tacacatatc ctgaaccaac gaaaacccca ccaaaaaaga
    12181 tagacaagaa agcaagcaag ataacatctt ttaactgcca ttttttcaac ataaaaaact
    12241 ccttttttta aagaaaagtg gggcactcaa gaagaccgac ctaaatactt tgtatagcag
    12301 actgaattta gaacagtaca caagaacact aaaatatttc tagaaattaa tttgaatttt
    12361 ctaattgatt tgttcgcatc ttatttcaat ctactatatc atcttcatcc agtttcgtaa
    12421 aagaaaaaac tctaattaca gatacaaatt agagttcagc ttacaagatt agacagttct
    12481 tttcgacata cgaaaaaaac atttcacatt tcccttcgcc agtcttaact gtatcaggtt
    12541 caatgggtat catctcagcc taaagcaccc caaatgtctt tattatttaa ttatgtgatt
    12601 attataacac acattttata ctagttcaag aaattgaact ggaaatacag ccttgcactc
    12661 acaaagacag cagatctttc ttttgcaaaa aacaaatgac ctgtttgatg aattagccat
    12721 tcaagctgaa tctggacata gctttttaaa aaaggaaaat cctacttact tagaatccaa
    12781 ggatagatat ctattgttca ctcatttccc gaacagtttt ttctatattt tttgcatacg
    12841 atattgccga aatgattgaa acgccatcca tattggtctt tataatgtct ttaatatgtt
    12901 tcgtctgtat cccaccaatt gcaactaaag gcatttgtgg caatagtttt ctcatcaatt
    12961 caagaccttc ataacctata gtaccaccag catcatcctt tgactgggta ccaaatacag
    13021 gcccaacacc tacataatct acatattcaa cttttgattg ttgaaattct tcttcgtttc
    13081 ttatagaaag accaattatt ttatctggca tcaattttct aatttcatca acaccaatat
    13141 catcttgacc tacatgtacg ccatcggcgt caatttccat tgctaaatct atatcgtcat
    13201 taacgataaa tggaacattg tattttttac aaagttcttt aatttggata gctagctcaa
    13261 gtttttctaa gccttctaaa gcaccctcac ctttttctcg aaattgaaat aaggttatac
    13321 caccttttaa ggcttcctca acgactgtat atagattttt tccttggcaa gtagtcgttc
    13381 cacaaataaa atatagtttt agtaattctt tatgaaacat cttacttcac tcttttgaat
    13441 tcctttacat cttcatctgt aatctcgtat aaggcattta taaattcaac tttaaatgtc
    13501 ccaggaagat gtccatttgg acgtttttct gccatttctc cagcgatatt gtaaaccaac
    13561 attgctgttt ctaatgattt caattcttga cctttttcta gtccgataaa gcttgctact
    13621 acagctccta ataagcatcc tgtcccaatg actttcggca tcatagcact accattatga
    13681 atcattacca cttctccatt aacagcaatg gcatccactt cacctgttac tactattgga
    13741 atattgaact tctcatttgc tgctagagca atttcgtcaa tattatctac gcccgcacta
    13801 tctactcctt tagatgccac atctattcct actaaagagg caatctcgcc agcatttcct
    13861 ctaatcgctg ctagtttata attgttgatt agatcatctg ctactttttt tctatattct
    13921 cctgctccac aggctacagg atctaaaact gctgggacat tatatttctc tgcaattttc
    13981 agagcagctt ggtataattt ccaattttca tctgtcaatg ttcctatgtt tattaataaa
    14041 ccaccagcat actttaacaa atcctctaaa tctgctggaa actcactcat ggctggtgag
    14101 gcgcccagtg ctactaatcc atttgctgtg aaatttttta ctacatcatt ggttatacaa
    14161 atgaccaatg gtgctttttc ttttaataat tttaaacttg tcatattgaa atccttcctt
    14221 ttcactttat acgatctact gatttcgatt tatttctatc tttcgttaag aatttttttc
    14281 atttacattg aatgatttat actcaatgaa aatcaaagag caaactagga ggctaaccgc
    14341 aggttgctca aaacactgtt ttgaggttgt ggatagaact gacgtggttt gaagagattt
    14401 tcgaagagtc ttacctcatc aaatttgtaa atatcatgag ccttctctag acatcgtaac
    14461 caatatcaaa aaaagctaat tctaaagcga ctgcttgatt ccagcgttgc tgaagttctg
    14521 tcaaatcttc tcgattttta tcgacacgat tgagttcgtc aaccagaaat tgaacccact
    14581 ctgcaaagaa aggacctctg tggagattga tccattccga atgaatatag acttcaggta
    14641 aagccaaatc tttagaaccc cagtctaaat agagaccttc tgcaatgacc agcatgacca
    14701 aaagatgggc atagtctgat gaagccaccg ccgaatacat tagatcctga aaggcttttg
    14761 ttacagggtg caaagtcact tctagatagt cattctctgc tacttttaac tctttaaaag
    14821 ccttttggaa ataaccatct tcatctgctt caagaaagcc tagttgcttg gcaaaacgaa
    14881 gcttggattc aagtttatct gcgtgagcta cgcaggcacc cagcatggat aagaaggcat
    14941 caaagaagtg ataatcttga atcagatagt cctttaagac cttattctca attgtccccg
    15001 caaaaagttc cttaacaaaa cgatgattga ttgcagcctg ccaatccttc tgactgcttt
    15061 ttaataattc tccaacagtc aaacctggct gaaatgcata gtcttgtgtt tccatattta
    15121 cttctcctct ctttacttgt tagtaattaa taaaacacca agaaatatca agcaaaatcg
    15181 taattccact tgatcctttt aaagcacatc gagagcattt gcagagagct aactaaacaa
    15241 gcctatccag tttatataaa caaaaaactc caattacaat caagaattag agttgactta
    15301 caagattaga tcgttcattt cgccatacga aaaaacgttt cacatttccc ttcgccagtc
    15361 ttaactgtat caggttcaat gggtattatc tcagcctaaa gcaccccaaa tgtctctatt
    15421 atttaattac tgaaccagta tagcaaaaaa tgaaagccct agcaagatat ttgaccgaaa
    15481 aatatcttta tatataatat attgaaacta gaatagtaca cctctacttc taaaacattg
    15541 ttagaaatcg atttgactgt cctgattgat ttgtcctatt cttatttcat tttactatag
    15601 ttttcgatag caatttattc ttccaataca cgaagaaaaa cctccacatt cagtggaggc
    15661 aatctgtttt atcaatacaa ttttaagtca cgagggtcaa ctgggaaggt tgggttgtat
    15721 ggattgtgac ggagcttaaa gtgtttgaca tcttcaatgg tctgagctcc agacaattgc
    15781 ataactgtct tcaattccgc attcaagtgt tcaaagactt gacgcacacc gacactacca
    15841 ccgagagcca agccataaat aacagggcga ccaatagcta ccaagtctgc tcctgaagcc
    15901 aaggctttaa agacgtgttg accacgacga acaccagagt caaagacaat cggcacacgt
    15961 ctatcaactg cttctgccac ttcttgaagc gagtcaaagg cagctggtcc accgtcgatt
    16021 tgacgaccac cgtggttggt tacccagata ccagaagctc ctgcagcaag tgaacgttca
    16081 acgtcctcac ggcattgtgg tcccttgaca tacacaggaa gaccagagta ttcagcgata
    16141 aattctacat cgcgtggaga caagcgttgt ttagctgatt tgtaaacaaa gtccattgat
    16201 ttaccagcac cttctggcag gtattcttca acaatcggca tgccaactgg gaagacaaaa
    16261 ccattacgct tatccacttc acgattcccc cctacagtag catctgccgt caagacaatc
    16321 gctttataac cttcagcctt cacacggtcc atgatgtggc ggttgatacc gtcatcctta
    16381 ctaaagtaaa attggaacca atggggtgtc ccttgaaggg cttcagaaat ctctggaagg
    16441 tcaacagtag agtaagagct ggttgtataa agagaaccaa actcatgcac accacgcgca
    16501 gtcgccactt ccccctgttc atttgccaat ttatgagccg caacaggcgc cataatgatt
    16561 ggtgaagata gtttttcacc tgcaaattca atctctgtac ttggattttc aacatcgcaa
    16621 agtgtatgag gaacgatgag tttgtggtta aaggcgcgga tattctctct taaagtgaaa
    16681 gtatcttccg ccccactagc gatatagcca aatgctgctt taggaataac ttgttgcgcc
    16741 attggctcca aatcataggt attgatgaag tctacatgac cttctgcatt gcttgttttg
    16801 tatgacataa aatgccctcc ttgattaagt aagcgtttgc tttttactac acaactataa
    16861 taactctttt ttcaatcatt ttcaagagtt ttgcttaaaa accatcttat tttttcatac
    16921 aatatactag ccctactttc cttaaactct aaactttagt ctatattaag tgaatcgttg
    16981 agattttttg ctgccaggca tttgaactac ttttttattt gcggattcta tctactaata
    17041 ctataaaaaa acaagtgaat aagacaccag aacatcctct aataggacct atggacttat
    17101 atcagtccca tatatactgt ttaagcaaga tactatccta gttgacgcta acttcctatc
    17161 ccacttatta attttttatt tatagtgaat ctgaacaaat caattgggga atccaagtca
    17221 atttctagta ctttttgaac agtttcagcg tactatcgta aaatgaaata aaacatgcac
    17281 aaattgctta gggaatttaa accaatttct aacaatgtct tagaaatcaa agtgtactat
    17341 ttcaacttca atatactaca cttcattttc tccttaaatt cagtctcttt ttgaatcaaa
    17401 aaatcccctg gtaagagcca gaggataaga atttatttca ttgttgggaa gagcaataca
    17461 tcacggatag ttgttgtatc agtgaggagc atgcagagac ggtcgatacc gattcccaaa
    17521 ccacctgttg gtggcatacc gtattcaaga gcttcaatgt agtcatagtc gatgcctgtc
    17581 gcttcatcat caccaagttc tttggcttta gcttgggctt caaaacggct aagttggtcg
    17641 attgggtcgt tcaactcagt aaaggcatta ccgtactcct tagtcatgat aaagagctcg
    17701 aaacggtcag taaagcgttg gtcttcagga tttttcttag cgagtggaga tacagctact
    17761 ggatgtccat agacaaaggt tggttggatt aaagtttctt caacaaactc ttcaaagaag
    17821 gcattgatga tgtgaccaac ctcagtgtag tgtttctcaa ctggaacttt cttctcagca
    17881 gcgatagttt tagcttcttc caaagtcatg tcttgccaga aatcgacacc agtaatttct
    17941 ctgatagcat ccaccatatg aacacgctta aatggttcgt taatcttgat ttcagtacct
    18001 tggtagttga ctgggccatc acctttgact gatttagcag cgtgttggat aatgccttca
    18061 gtcaagtcca tgatgtcttg gaagtctgca taagcttggt aaacttcgat agaagtgaac
    18121 tcagggttat gagtagcgtc cattccttcg ttacggaaga tacggccaat ttcatagaca
    18181 cgttccatac cacccacgat aaggcgtttc aagtgaagct cagtcgcgat acgaagcacc
    18241 atgtcaatgt tttgggcatt gtggtgggtg ataaatggac gggcagcagc accaccggct
    18301 tcattatgaa gaacaggtgt ttccacttca aggaatcctt tttggtcaag gtaacgacgg
    18361 atttcagaga tgatttttga acgagtgaca aagcgttcaa agctttcacg attagaaatc
    18421 aagtcaaggt aacgtttacg gtaaattgtt tcaacgtctg tcaaaccatg gaatttctca
    18481 ggaagaggac gaagagcctt agacaagtgt gtgatgtggg ttgccttgat agagagttct
    18541 cccatatccg tacgcatcac ttcaccttcg acaccaagga agtcaccaag gtctgctttt
    18601 ttgaagattt cgtagttttc ttcaccgaca gcatccttac gaacgtagat ctgaatctgg
    18661 ccttcgcggt cttgaaggtg ggcaaaacca acttttcctt taccacgttt ggttatcaag
    18721 cgtcctgcga tagtagctgt ttcgttttta tcgtgtaatt gttctttatc gaggttggca
    18781 tatttatctt ttaattcttg tgaatttgca gtacgttcaa aacgttttcc gaaaggatcg
    18841 attccttgtt cgcggagcgc agccattttt tcacggcgaa cgatctgctg gtcatttagt
    18901 tcttccatat gttctgtaga catgggatcc tcctagtttt actcaaaatt gaatacgatg
    18961 agtcattttt tgcgatactc ccattttatc ataaataagc aagaaattca cggataatct
    19021 ttaaaaacta aaaagaacct gacgctaaaa tcactggaaa gaagtgaaag tatctcccgc
    19081 cccactagcg atatagccaa atgctgcttt aggaataact tgttgcgcca ttggctccaa
    19141 atcataggta ttgatgaaat ctacatgacc ttctgcattg cttgttttgt atgacataaa
    19201 atgtcctcct taataagtaa gcgtttactt tgtgtattac aaaaatatct taactctttt
    19261 tcaaaacttt taaaatattt tgtttggaaa tttcagaaat tttatgtcta tgataaaaat
    19321 ccttataacg gcaataaaaa atagatatta tccaaagaag attttaagtg ctacaataac
    19381 tgtattattt ctagatggga ggttctattt ttggattgat ccattgttga acaatatcta
    19441 ccactatatc aaaaggcatt ctttctgacc ttgcatattg cagtttgggg aattttggga
    19501 tcctttctgc tcggtttaat cgttagtatc atccgacatt atcgaatcct tgttttggcg
    19561 caagtagcga cagcctacat tgaattgtca cgtaatacgc cccttttgat tcaactcttc
    19621 tttctctact tcggtcttcc ccgaatcggg attgtcctat cttcagaagt ctgtgcaacg
    19681 cttgggcttg tctttttagg aggctcctat atggcagaat ctttccgaag tgggctggaa
    19741 gccatcagtc aaacccagca ggagattggc ctcgctattg gtctgacacc tctacaggtc
    19801 ttttactatg tggttcttcc gcaagcaaca gcggtggcac tcccctcctt tagtgccaat
    19861 gtcattttcc ttatcaagga aacctctgtt ttctcagcag tggctttggc cgacctcatg
    19921 tacgtcgcca aggatttgat tggtctctac tatgagacag acattgcgct agctatgttg
    19981 gtagttgctt atctgatcat gctgctaccc atctcactgg tctttagctg gatagaaagg
    20041 aggctccgcc atgcaggatt cgggaatcca agtactcttt caaggaaata atctcctgag
    20101 aatcttacag ggattgggcg ttacgattga gatatccatc ctgtctgtcc tcttatccat
    20161 gatgttcgga acagtcatgg gaatcatcat gacctcccat tctagaatca tacgattttt
    20221 aacacgattg tatctggact ttacccgtat catgccccag ctggtgctac tcttcatcgt
    20281 ttactttggc ttagctcgaa actttaatat caatatctca ggtgagactt cagctattat
    20341 cgtttttacc ctctggggag cagctgaaat gggagacttg gtacgtggag ctatcacttc
    20401 tctccctaaa catcagtttg aaagtggaca ggcactcggc ttgactaatg ttcaacttta
    20461 ctaccacatc atcatcccac aagtcttaag aagactgcta ccgcaggcta tcaatcttgt
    20521 cactcggatg attaaaacca cttcattagt tgttttgatt ggggttgtgg aagtgaccaa
    20581 agttggacaa caaatcatcg atagcaatcg cctgaccatc ccaactgctt cattttggat
    20641 ttatggaacc attctagtct tatatttcgc agtttgctac cctatttcca aactatccac
    20701 tcacttagaa aaacattgga gaaactaaat gtctgaaact atcttagaaa tcaaggaact
    20761 aaaaaaatcc ttcggagaca atcccatcct ccaaggactt tctctagaaa tcaaaaaagg
    20821 gaagttgttg tcatcctagg gccatctggt tgtgggaaaa gtaccctcct tcgttgcctc
    20881 aacggcttag aaagtattca aggtggagat attcttctgg atggtcagtc tatcgttgaa
    20941 aataaaaaag attttcacct agttcgccaa aagattggca tggtctttca aagttatgaa
    21001 ctctttcccc atctggatgt cttacaaaac ctcatcctag gccctatcaa agctcaagga
    21061 agggacaaga aagaagtaac ggaagaagct ttgcaattac tagagcgtgt cggtttgctg
    21121 gataaacaac atagctttgc ccgtcaatta tctggtggac agaagcaacg tgttgcaatt
    21181 gtccgtgccc tcctaatgca tccagaaatc atcctttttg acgaggtgac tgcttcgctg
    21241 gatccagaaa tggtgcgtga ggtgctggaa cttatcaatg atttggccca agaaggccgt
    21301 accatgattt tagtaaccca cgaaatgcag tttgcccaag ccattgctga ccggattatc
    21361 ttcctcgacc aagggaaaat cgctgaagaa ggaacagctc aagccttctt taccaatccg
    21421 caaaccaaac gagcccagga atttttaaac gtctttgact ttagccaatt cggctcatat
    21481 ctataaagga gattcttatg aaactattca aaccactctt aactgtttta gcacttgcct
    21541 ttgcccttat ctttatcact gcttgtagct caggtggaaa cgctggttca tcctctggaa
    21601 aaacaactgc caaagctcgc actatcgatg aaatcaaaaa aaaagcggtg aactgcgaat
    21661 cgccgtgttt ggagataaaa aaccgtttgg ctacgttgac aatgatggtt cttaccaagg
    21721 ctacgatatt gaactaggga accaactagc tcaagacctt ggtgtcaagg ttaaatacat
    21781 ttcagtcgat gctgccaacc gtgcggaata cttgatttca aacaaggtag atattactct
    21841 tgctaacttt acagtaactg acgaacgtaa gaaacaagtt gattttgccc ttccatatat
    21901 gaaagtttct ctgggtgtcg tatcacctaa gactggtctc attacagacg tcaaacaact
    21961 tgaaggtaaa accttaattg tcacaaaagg aacgactgct gagacttatt ttgaaaagaa
    22021 tcatccagaa atcaaactcc aaaaatacga ccaatacagt gactcttacc aagctcttct
    22081 tgacggacgt ggagatgcct tttcaactga caatacggaa gttctagctt gggcgcttga
    22141 aaataaagga tttgaagtag gaattacttc cctcggtgat cccgatacca ttgcggcagc
    22201 agttcaaaaa ggcaaccaag aattgctaga cttcatcaat aaagatattg aaaaattagc
    22261 aaggaaaact tcttccacaa ggcctatgaa aaaacacttc acccaaccta cggtgacgct
    22321 gctaaagcag atgacctggt tgttgaaggt ggtcattaac tcttaaaagg gactggattt
    22381 taagctccaa tcccttttta agattttacc tataacatcc tgagtctatc taagatgttc
    22441 aatctgaaca cagtgtacat actttatctt ctattgcata tactttatca cataagatac
    22501 gaatatcctc ttcactatga ctagcaatca aaattgttgt ccctttttca ctagagagct
    22561 ttctaaacaa tgttctcata ttttctacac ttgatttatc caaggcattc ataggttcat
    22621 ctagtaaaag aatagaggga ttctccatga ttgcttgagc aatccctagc tttttcctca
    22681 tacctagcga ataagtttta actttctggt ctttttgctc atatagacca actattttca
    22741 gtgtatcatt gatttcctga ttaccaacta ctcctcgtat gcttgccaaa tattgtaaat
    22801 tcttaaagcc actataataa tttataaaac caggttcttc aatcaaagct cccaaattag
    22861 ctggaatttt tctctcagga acaatatttt ccccattgat taacacttct ccataagacg
    22921 gactatataa accagctatt aatttaaaca atacactttt ccctgagcca ttcgcaccag
    22981 taattcctat aatttccccc tgtttacaac taaagttaag gttttgaaaa acacatgtct
    23041 tttttaattt caactcaata ttttttaata taattatttc attcattcta taaacctcct
    23101 cttttgacga gtgaaataga aaatgctttg aaaaagaaag actaaaaata gcaactgaag
    23161 aaacaaatct ccccctacat ccccattccc tcgattcaaa atataaaata gataattagt
    23221 tcgatttcct acaaatagac caccaaacac aatcatgagt aaaaagaaac taacgcaagc
    23281 aaagttcatt tgtacattcg ctcctagtgc gtataacata aagcaaaaca agactaacac
    23341 acaagcattc aaagaatcaa ctataaacaa aactcctata tcccagtttg gtaactgtaa
    23401 cataaattga atcactagaa cttttaccat gattaaaatg agtccataaa gaaaacaggt
    23461 ccaaatctga tacctaatcc agagcttaat agaagaaaat cgaatcactt ccataaagaa
    23521 atcggatgct cttctacgat atacaactgc tagaaaaaat gtatgaataa taaaccaatg
    23581 aagcaaccat atagataaga ctaaaaaaga gttttgactc agattaggcc cttttaaaat
    23641 ttctataaca agtctatcat ttccttctaa ccaataccat aatcctaaac aaatcaatat
    23701 ccaaaaaaga acataacccc aaaagtactt ggtatagatt atcttattca acctccacat
    23761 gtgactactt aactttccca tttccaactt ttacactctc ctccagtttc tatgaacaga
    23821 aaatacagtc aatggaatta tgatagatag taatgtaagc caataaatca atgcatacgg
    23881 attggaattt accatatact gaaaaattgg caataatcca attatcccat tgttcaactg
    23941 ataaatccag aataaggatt ctacaaataa aaaagtaaac agaaatacta agctcatgtt
    24001 tgatttttta aaccaagaaa aactgctaat agtaagtaag gataaaaaga aatagtatgc
    24061 tatataagag aaaaaaaatc ctataaagaa actagcattg tttgcaatac ttataccata
    24121 aaattctctt aaaaaatcaa cctcctttat ctccaaagag aagctaaaac cattactaaa
    24181 tgcaattaga aaaatcaata aaaataaagt cgccgtccaa atccccgtac taagagctgc
    24241 taatttgaaa ctaaaactgg taaagtgctt aattgatttc agacgaatgc gacactccaa
    24301 cctattaaaa tagttattca tcaaataaaa aaagaacaat atatatgtga acggaaagca
    24361 atatactcca gtcgtcatat cttgaagtaa aactaaaatc cattctaata catttggatg
    24421 gattgaatat tggcgacagc gcaataaata tactgtacta gataaaacac aggatagcag
    24481 taatataaaa taaaccaata ctgataaaaa atcttttttg taaaattgaa caaattgttt
    24541 cattatacat agtcctctga atgtagaaaa aatgtaccat aaacaaccaa acaactaaca
    24601 aataaaataa aagcaagatg cccactaact aaggaaagac tgatatcttt ctgatatccc
    24661 aaagctaatg ttgtcacagg ttctaagtaa gatagcccta aaatagccca aaaaatacca
    24721 ccaaccatca tataggcaac tgggatgaaa atagctccta tttttttctt cactagcaaa
    24781 gcactagcta gtccaaaaat agagaacaca gcgccccaaa ctccatacca gagagtcgtc
    24841 acaagactat agagcaactg attagaatca aataattctt ttaaggcacc actataatct
    24901 ccaatataaa tttcctgata aggagtcact aaaagattaa ttcctaataa taataaatag
    24961 gggagaaaaa agactagaaa agaagaaata attgcagtac tacctacaat agccagatac
    25021 ttctttttag aaattcggca caattgtgct gttagaaaat gactctcagc atcctctatt
    25081 atctgactag aatagggcag tgtacagata agtgcagcta ctaggctaat cggtgaaaat
    25141 cctcgaatag aagaagcggc aaaaaaaatc gataaacctt caattttata aataccattg
    25201 aaagcaagga aatttcctaa actcatgcaa agaagggtca aaaataaaga catataaaat
    25261 cgaggtgatt gaacgactcg tacaagatta caaatgaaaa attccatcct tactcctcct
    25321 tataataaaa atagggtgta gcattctttt ttcatgctac acccacaatc aaccatcttt
    25381 aaggcttact ctgacaagta agttaataag aatctggact ccaagaacct gaagtatgaa
    25441 ttcttacatg atttccaaat tgtggcgcca tagctaatct agtaccagaa ccaatataat
    25501 tgtcaccacc tccattatag tacatgacaa tcctagagcc agaccccaat gaatataccg
    25561 gggtaatatc tgacccacta taggcgctac gaatagaggt atttaacctt ttaccgccac
    25621 cagtgctgtc actattatta attccagcag aggcgttttc tttctcaagc tcactagttg
    25681 tatccgaacc ccaacgcggc aacgttaaat cataatttcc aatctgtcta gctaaaactg
    25741 tagggagaaa caaactagat gcaattgtaa ctacgcaaaa aaggaatata tttttcttct
    25801 tttcatgaca tcgtcctctt aagaagtgat aaacaagata gttgattgat caatttgata
    25861 catattacat tttcattata tcaataaaag aaagcgttgt caattatttt atttaaaata
    25921 ttgtcatatt ttaaataaaa tggtaaaaga gactcggtag cctcgtctct ctttatctta
    25981 ctgacctaac cttcttgcca attttcttgg tcttctttaa agcgttttag aagatcaagg
    26041 ccctctggcg tgatgtaacc ttcttcttgg gctagatgga taagctctct atagtttgaa
    26101 agcgtcacaa gtttaacacc agcatctgca aagttcttat ctgcttttgg caattggtag
    26161 ctgaaaatcg ctacaactcc aagtacatct gctccttctc gcttggctgc tgctacagct
    26221 tcaagaactg aaccacccgt tgaaataagg tcttcaacca ctaccatttt ttgaccttga
    26281 gctacgcgac cttcgatttg attaccagct ccgtggtctt ttggtttgct acggatgtag
    26341 gcaaaaggca agtccatctt atcagcaata atggctccgt gtggaatccc tgctgttgca
    26401 gttcctgcaa tcacttctac ttcaggaaag gcttctttaa tagcttccac aaaaccattt
    26461 tcaattaggg tacgagtttc tggataggct agtgtcacac gattatcagt gtaaatcggt
    26521 gacttgatac cagatgccca agtgaagggt tcctctggtt tgaggtaaac ggcttggatt
    26581 ttcaagaggt ggctagcgat atctttagca agtgtcatgg tattctcctt ttatttttct
    26641 aatctagttc tttaattcca gttctgtgtc cattcatcct tgatggcatg ataagctgca
    26701 acaggatcct cagcttgggt aatgggacgt cccactacga tatagtcact gccgatttga
    26761 taggcatcag caggtgtcat gactcgtttt tgatctccaa ctgcaacacc agctggacga
    26821 atccctggtg tcagacagat aaaatctgga ttggtagcct gcttgatgac ttgtacttcc
    26881 tgagccgagc aaacaacacc atccaagcca gcttcagctg tcttcttggc atagtgaatc
    26941 acagactctt gcagactggt ttggatattt tgaaactcct gcatctgagc ttctgacgtt
    27001 gatgtgagct gagttacagc gatcaatttg gcttgactcc caagaccttc acgcgccgcc
    27061 ttcatcatct ctacaccacc agccgcatgg acattagtca tatcgacacc aagctgagac
    27121 aagaccttca tggctgactt gactgtatta ggaatgtcat gaagtttgag atccaaaaag
    27181 acactatgac ccaaaccttt taagtaggac acaatctcag gccccgctgc gtaataaagc
    27241 tccatcccta ccttgagata aaggctttct tctgctggga aaagagctaa aaattccttg
    27301 accgcctcaa aactaggaaa atcaagagca atgattggac gatgttctcg cataggtttc
    27361 tccttttacc tttactagtg agagagtctg gccaacagaa gccacgaaaa aaggaacctg
    27421 ccaacagcaa ggttccacga aaaatgggta gtctccaccc tttcaccgtc taaccttagc
    27481 tgcctctctg gactgcttta aaggttttct attattctat tatttctact agcacttgtc
    27541 aagtaaaaac ttagtcacgt atgtgttgac tgctattgaa cacctcacaa gactctttcc
    27601 aaaagaatag ttaaagactt tcacttagga cagagaaaaa gagtttgctt gttacccttt
    27661 aattgaacaa atgggaattc tgttttattt tgcggatgcc tattcatctt ggcaaagggg
    27721 aagtaatgag aatgcgaatg gacttcttcg tgagtatttt cttaaaccga tttggcaaca
    27781 attaccgaaa aaatccttaa tggtgctttg ctagcttatt aatcaccgtc cccaaaaata
    27841 cttggattac aagacaggtt ttgaagcact tatagaggaa atttaaaaat gttgcatttt
    27901 ttattgcaat ttatcagtta tcttatcacc caaaacttct gcaattagta tgagcactat
    27961 tgttattttt cttttttcgt acactcaaat caaaatatag caataagact attctgtaga
    28021 attgctgtca aaaagatcag ttcatttttc cttcccactt gtgtttataa tcattaaaat
    28081 aactcaatta agaaattttc tttttcctaa tagttatcgt aattaaagtt aatagaagaa
    28141 caatccaaat aagtagtgtt agattaggaa ttagagtttt attccatgaa aatccctccc
    28201 attttgtgaa aaacttagta aacagtccca taaaactata atctgttatc ttcgcaactg
    28261 tcttatccaa accagatagc atcggtaaga aagcaactaa aatcatagca ggaagactga
    28321 taacctgagc tactacttgg ctcttcgcgg ttaaacctat caataaatag aataaaatga
    28381 tggataaaga ggttagaaga acgattgtaa tataattaaa agtatgtaca attgtaactc
    28441 ctaaaataag aggagtagtt cccataatca caaaagttag caaaaaagga agaaacatcg
    28501 ttgataaaat gtattcggat cctttaacac cactcaacag aagagtttgt aaattgtact
    28561 tttctttttc ttcagacaag ataatagtta taggacttcc aacagccaaa gccaaagaaa
    28621 aaggtaaaca catcatcaaa agaactaatg cctgttgatc gttgaccttc ccctgtgttt
    28681 ccataagata tttataaaaa tatgtgaaag caaaaggcac taaaacttgc aataaaatac
    28741 tcttattgga aaggataatt tgacaacgca accataccaa agccagaaac cgtttaagca
    28801 tttagctttc ctcctgttaa tgtgataaaa atatcttcta aagtaggctc acatgaatga
    28861 attgaaaaaa tattttccag atcagcctga gatacctgtt ctagtgatgt aaaatcaaaa
    28921 gttatctgat tcccattata atctgtaacc ttaatctttt tatctttatt atatctttga
    28981 atgagttcag aaggagctcc ttgctcaact aatttccctt tatttaataa ggcaacataa
    29041 tcacataaaa gagttgcttc attcatatca tgagtcgtta gaaaaatcgt tgtccctgct
    29101 gttttcaatt ctaaaattaa ctcatgaatt gttcgagaag ttgtgggatc tagacctgag
    29161 gtcggttcat ccagaaagag tacagcgggt ttgttgataa gagctcgtgc taaaagcatt
    29221 cgttgcctca ttccagtgga taattttcct gctaccatct tgcgactatc atataatcct
    29281 actcgcttta acaaattatc aacacgtgat ttactaatat tataaaattt actataaaaa
    29341 agaagattgt tatacagaga cattttctca taaaatccac ttgtatcgct aaccaatcca
    29401 attctcttta attcaccgct tgttaaattt tgagattttt gtcccaaaat aatagattgt
    29461 cctttatcgg caaggaactg cccagtcaga atattaatcg ttgtggtctt tccagaacca
    29521 gatggtccta aaaatctaac cttctttaac agtgaagctt atattattta aagctatttt
    29581 atcaccaaaa ctttttgata aatggctaac ttcaatcata ctcattgcac ctcctttata
    29641 ttctacattt ttacttaaca tctatttact tatctttatc aaataaacgt cttaaaaaaa
    29701 acaataacat taagcatata accgttagca agattgtttc aatattattg ataatacgat
    29761 acattctcaa gcagtataaa ataattatat atatgcaaat gctcattata cctatttttt
    29821 taaacatact aagcctcctt gctaaataat aaagttatat aataactaag acacactata
    29881 cctaacataa taaatatcca ctggtaactg aataaatgtt ctaaaattaa caagcaaaaa
    29941 taaaatctca aacgatgaaa aaagccatac tatattttcc aatgatgttt gaatattttt
    30001 attgtagtaa gcataatata tagcaaaaat aatacccaag aacaatacta ttaattgtgt
    30061 ttctataaca gtgaactgcc caatagcaga taaaaaagct actcctcctg ataataagca
    30121 aaaacaaact gaacgaataa atgttttcgc tctaatattg ttatctttca ctttattatt
    30181 tttttacaaa ttggacattc tccgttttcc agaacatatt tttcttgact tagagcatga
    30241 tttctcatat tataaacgta agcgttatct attaggattt cattataaca tcccagaaat
    30301 attttaatta ttaaattagt cattatccct cccaaaatag caggcatctc tggtgtagtc
    30361 ggccactttg tcttatcaga taaagtgtac ttatttatat ctttgtttat attccgacaa
    30421 ccaaagcaag aaccgatggt agggatatat attatcagta ataagatagc atccattaaa
    30481 ccctcctgat atgtaggata tcttatgcga tacagcaaat tgattgacaa tttttataat
    30541 atcaatgggc gtatcaattg ctttaacgat aaaatctata ctcccatatt ccgcaacaat
    30601 tttttctaat tcttctactg aagaaacttt aatagaaata ggtactacct gaacatctga
    30661 attatacttg tgtatttttt cagaaagaac attaatcttc tccttaccaa catccgactc
    30721 tgtataaaga atttgcctat ttaaatttga tggttctatt atatcgtaat cagcgacaat
    30781 aagtttcctc actccataca tacttagact tagagatata taacatccta aggctcctgc
    30841 tcctaatact aatactgttt tattctgtat tttattatac tcttcattat aaaataacga
    30901 atagtaacct ttagttctag aatatctact atcaatatct atctcatccg aaattaaaat
    30961 ttgttcattt aatagttcaa atgttagttt actgcctaat acttcttcta tctctaatag
    31021 ttccttacaa ctattaaata gatcgttgat aaattcttgc gaaatttgag gttctatagc
    31081 tatttcccgc atactccgac cacgcaaata cattatacct tcttttttat aagcaaaaac
    31141 ataactattt acgtagaaca tatttcgtcc ttcctctcat atataatata gaaagcagta
    31201 tagaaacaac cacccctcct atactacgaa caataatatt aggtgtcaaa aactgtaata
    31261 gttgactcaa agggaaataa ataataacaa atgctattaa aagttgaact atatattgaa
    31321 aattcaagtt attattgtta gaattcaagt atcttaaaat aataccacta tcatactgaa
    31381 aaaaaggaat tgaattaata aaaatcatac ctaagttaat gataaaaaat aagagtccaa
    31441 gcaatttata cgatgtgaac aataaaacaa gtaaacctgt aagtgataaa atcgaatttg
    31501 aagcaattcc tgctaacata attttcaaaa attgtttttt attttttaag aattgcaccc
    31561 cacttaaatc tacaaaaaaa agccaaatta aacaaaagta gcattattcc acaatttggg
    31621 atctcaattt gataattatt agcaattatt gcatgactaa attcatgtat cgttgcagac
    31681 ataataacaa caagcaacca aaataaaaga atgtttagcg ttattggaaa gttttggaaa
    31741 ttagtcaaaa tatccgccct ataaatatac ataataaaac attgcaaaat aagagccaat
    31801 gggaaaatac caaataggat tttttcaaaa ttcttattaa atcttttata gtttaatttt
    31861 tcagataatg ttatattaaa gtcccaagat attttaaaaa tatttatctt ttttcgtgat
    31921 ttcatagaat ctataagtcc cattttacta agaacaatat aaaatcttaa ataatatttc
    31981 ttccctacta acactaataa gtccgatatg gtaaattctt gacctattag ttgctctaac
    32041 catttttttc ctgtttccca actaatacat gtttcttact agatggtata tatagatgat
    32101 actgtccctc ataaggagta ggtaatatct taatcttatc aacaattctc ataacaaacc
    32161 taacctttaa taaataaaag aggggctaag taaaacatac tctagtccct cagtccgcaa
    32221 tcaataaaat aatgactaat tctattaatc tcttgctgca agactaatac ggaaaattca
    32281 aatgactgtc taaaatgaaa ttgaaaaagc ttgatgttag taaatgaaca aacttcaaca
    32341 accagcaaga gcaaggattg ctaaaccaaa tccgatacct gttgcaagtc cagctgaaaa
    32401 atcactaact ggtactgaaa tttcttcaat ttcctcaaat aaaaagttat cttgcatgat
    32461 tatcactccc tatcttttta acaacaggct attccaatta agagaactac cccagttcca
    32521 agtaaaactc ccccaaaaat tcaccatctg taggggctac gatttcttca atttcttcaa
    32581 ataagaatgc ttcatctgac atatgtgtta ccttcttcat aaaaaattat tttgtaatcg
    32641 attacattgc aaatgtaaca taaagaaaaa cagatgtcaa atattaaacg taagaacatg
    32701 gtcactaaag aactataaga gaaaaggtaa acctagcgac gcgatgagcg ctgggtcgtt
    32761 tgatttcgat tgctctcttc ctcttgtttt ttctgttctt cttcctgttt tttctcggct
    32821 tccttggcct cttgtttggc tttttcctca gcctccataa ttaatttatc cgccacagtg
    32881 taactgtaaa ttccagcttc catgtcgacc acactcggtt ctgacaattg tggcttaatc
    32941 ttactgtaat atggcagttt cttactcatt tcagatagag gaaccaagac ttcgtccgaa
    33001 tcattcatgg tcaatcgaat taaatcggat gtcaccttgc ttggggctaa ttccaccttt
    33061 tggatagccg ccttgagttc tgggctaatt tgagcaagtt ctgagacaaa aaccttgatt
    33121 tgttcactat cattaaagag aactgataaa taagtttctg gtaaactgtt cagactcaca
    33181 gaactagtct caagctgacc actggaaaga ataggataat gattttcacc agaaatatag
    33241 taggccacaa tatcatattc cttgacctta atagtgaact tagttggaaa ttgatagaca
    33301 agttgagctg attcaaccca atagttagac ttaatctgct tttcatattt tgccttgtct
    33361 agcagaaggt taatcgtata atccgaatcc tgaatgcctg aagcctgtcg aatatcatca
    33421 gctgtagttt gcaccgttcc ctcaacacga atatctttca tggtcgcata aggactgagc
    33481 aagtaggcag agacaatcaa taaaagcaga cttggaaata aaatcgtgaa ggctcgcaag
    33541 atatggatac caggaatctt tgctttggct ggtttttcct ttgtagcctt tttagaaagc
    33601 tttttatcct gttcctcctt ctctttagac tctggttctt ctttctcttc tttctctttg
    33661 tcagcctctg aggatgctac tttttcttca gactcttcct tagctgattc tgaatcttcc
    33721 tggtctgttt cactctcctg gtcctgtttg tcctctgact tctcagattc ttctcccatt
    33781 cgagcttgtc tttccttttc cttctcctca gctagagccg cctcttcttc agccttcttt
    33841 tttagatatt cttggtttcg tttctgccat tctgataact ctttcaattc ttcgaggatt
    33901 tctttgtcct catttttctt atcttttgac atttactttc cttatgataa atcttttttc
    33961 aacaattgat aaaaatctgc tagagatttc aattccttag aagccttcat cttagcttgg
    34021 taatcttcct tgtgacttag taagtgagaa agcttctctt ccaaactatc caaggtcaaa
    34081 tcgctttctt gaaggtcttc tgcatagcct ttttttacaa agtaagctgc attttcaagc
    34141 tggtcaccac gactagcttc acgaccaagc ggcacaatga catgcaattt tgctatcgcc
    34201 aagagctcaa aaatcgtatt ggcaccacct cgtgtcacaa caatatcagc caattccatc
    34261 aagggttgat agagatcggt cacatagtca acacgaaaaa gattttggct caactcattc
    34321 aggctagaat ctccagttag attgataata ttgtagcgct ctgttagttc tttcttatgg
    34381 tctgtcacca attggttaaa gacacgagca cctgcagaac cgccaacaaa caatacagtc
    34441 ggcaatttat gattaaagtg gctttgaata tccaccaatt catctggttc tggatttttt
    34501 tgatctgaaa ccttggccac tgctcccaca tgctcaacct tagacaaact cgaagcttgc
    34561 tcaaaggttg aatacatctt agtcgcaaat ttataggcga ttttattggc caagcccata
    34621 gacaggtcag attcgtgaat aaagacaggc actcctgaca cacgcgcagc gataacaggc
    34681 ggtactgaga caaagccccc ctttgaaaaa agggtctgtg gacgaagtcg caacatgata
    34741 aagagcgatt ggacgattcc ccagcaaact ttgaagacgt ccagcatatt ttgccaagag
    34801 aaatagcgac gcaattttcc agtcgcaata gaatggaagg tgacatccaa acctgactta
    34861 aggatttctt ggtgttcgat accacgcttg tccccgatat agtggacttc ccaaccatct
    34921 tcgatgaact tgggcattaa caaaagattg agggtaacgt gtccaaccgt ccccccacct
    34981 gtaaagacaa tttttttcat attattcttt taactccgct actgtgtcga taaagaggtc
    35041 gccacgtact tcaaagttag catacatatc ccagctggca ttggcaggac taagaagaac
    35101 cacatctcct tgagtcgcaa gctcataggc cttgcgggtc gcatctgcaa tatctgtcgc
    35161 ctccacataa gcgacaccag ccttgtctgc tgcccgtttg acacgttctg cagattgacc
    35221 caggatgacc atcttcttga gtccagtaat gtctggcacc aattcgtcaa actcattgcc
    35281 acggtccaaa ccacctgcaa tcaagacgac cttgctgtta tcaaatcctg acaaggcttt
    35341 ttgagtagcc aagatattag ttgatttact gtcgttatag aatttaacac ccttgatgtc
    35401 atccacaaac tggagacggt gtttgacacc accgaaggct gaaagagttt ccttgatggt
    35461 ttgattgtcc acaccacgaa gcttggctac agcaatagtc gcaagggcat tttccacatt
    35521 gtggctacct ggaacaccga tttcattcgc tgccatgact acttcaccac ggaagtagag
    35581 ttgaccatct tccagataag ctccatcaac cttttcaagt gttgaaaatg gtacaacagt
    35641 ggcttctgtc ttggaagtca agtcttttgc caagtcttga ttaaagttca agacaaggaa
    35701 atcagctgct gtcatcttgt tctggatatt ccacttggct gctacatatt ccgaaaatga
    35761 cccatggtag tcgatatgag ttggcatgag gttggtaata accgcaatct ctggatggaa
    35821 ttcttgaaca cccatgagtt ggaaagaaga aagttccata acaagcgtgt ccttatctga
    35881 tgctatttga gcaacctgac tagctggata gccgatattc cctgataaaa gaccatgttg
    35941 cccagcagca gtcaaaactt ccccaatcat agtcgttgtg gttgtcttac cgttcgatcc
    36001 tgtgatacca ataatcggtg cttctgaaat caaataagcc aattccacct cagtcaagac
    36061 tggaattccc ttggccaaag ccttttcaat catgggattg ttgtagggga tacctggatt
    36121 tttcaccata agggcaaact cttcatccaa gagttccaaa ggatggccac ctgtaatgac
    36181 cttgatccct tcttccagca aactttgggc agctggattg tcctcgaaag gtttcccatc
    36241 atttactgtc acaatggcac ctagcttgtc caacaaacga gctgcagatt caccagactt
    36301 ggccaaacct aaaacaagga ctttcttatt tttaaattga tctattactt tcatgtctcg
    36361 aactccattt ctactcctac tattttacca tttttatgga aataaaaaag ccacaaagtg
    36421 tgtttgtgac tctttcttct aactgaatct taccatatca tctatgtgat aaatcggtaa
    36481 ctcgaatgac ctgatccact tgttcccaaa taagaggatt gtgggtcgca ataataatgg
    36541 tccgattcgg attttttaaa gattctagga tggaaagtaa ttcctcagag tttttggggt
    36601 ctaaggaagt ggttggttca tctgcgagga tcaaaggtgg atcctttaaa attatcttcg
    36661 ctaatgcaac acgttgtgct tctcctcctg ataactcaaa tataggttgc ttcaaatcca
    36721 aataagagag gtttacacag tttagagctt gtttcatcaa agagattttc tctttttcct
    36781 tcaacttttt accaactaaa cccagattga gattctcttt gacggtttgg ctttcaatta
    36841 agccaaaatc ttgaaataag tatcccaagt aatctctaaa gaaaacagaa ggcttgatgt
    36901 ccttaagaga agtaccatca tagatgattt gtcctttgtc atatggctct aatcgtccaa
    36961 tcatattcaa gaatgttgtc ttaccacagc cacttgtacc gattaaggca taaattttcc
    37021 caccttcaaa atgaagattt gtatctgaaa atagctgacg gcttccaaat tttttagata
    37081 tattctttag ttcaatcatc ctattttcct ttcataattg tcatagaaac acgagattct
    37141 ttctgcgctt gacggtaaag cgtcagaact gcactagcta gaaagactaa taaagtgagc
    37201 aagccaatca ccaagtctcg actgcttaaa ataaagagac tagcaccaaa tacaaaactg
    37261 gcaaattgac taaccatata ctgagcatgt gtttcaaaaa atcgtaaacc tgaaattcgt
    37321 ttaatcaaaa tatctcggcg gaattgctcg aaatatagaa gattgacaga ataaaatagt
    37381 aataaggaac tagctatccc cacaatagct cctaagatta aagttgctgt ttcagtttga
    37441 acttcattat aacgagttag ataaacactt cttccttctt taagatagga tacttgctca
    37501 taaattccag ctttcttcaa gagttctagc ccactctcat atcctttgat aaagagttgt
    37561 tttccagcat tgatagacca actagataag gatataaaac tatcacctgt agaagtcggc
    37621 gtgaatacca ctaaaatcgg atcagtcaaa tactgagtag atacgggatt ctcaccgtta
    37681 ttataaacaa accgcttttc tcccattgaa agataactaa cgtgcgcttt catctcataa
    37741 tccaaaggag cacttgcctc ctcaccagat tttccataat aactcaatct ttcttcaaaa
    37801 actttcttaa gttctgcttc tcgagagcgc aaatgttctg ggagcaagag gataaactca
    37861 cctttttgga gatgggctaa cttctgtttg gtctcagcat ctaccacgac cttttccttg
    37921 tccaaataac tgggactaac atagagcgta ttagcatctg gactataggt atccagtgtc
    37981 tctccctgtt cattttttcc ttgtggattg gcaaaatgga gcagattatc ctttacataa
    38041 agagcttgtt cttcttcgat tgcttccttg gcaaaggcat accacttgct ctgattttct
    38101 gtatcttttc ctctatcacc taagccaaaa gaaatttgat aatagtctgc tctatcctgc
    38161 catgcttgtt ttgaaatttc aagttctttc aatcgttggt aagacatcaa acctgtctta
    38221 acagcgtagc ctactgtaaa gacagcaacc aactgacaca atagggttaa aatcatcagg
    38281 cgtttaaggg gtaatctccc cttaataacg ggaactaatg ctttgtaact caaactcatt
    38341 agataaagaa gcattagtaa aattgaaatc gccaataaaa acaacagata gaaactaatc
    38401 ccaaaaccat aggtggctaa caagatagga taaaacaaac cttgactaaa aagaacgact
    38461 cccccaccta ggaaggaaag gagggctgat agaaggagcc atttgatatc agtagataaa
    38521 gaatgcccca tgatggataa gagagtctga ccagaaaaga gttttatacc tgctgctctc
    38581 atttccttaa tccgagtgat aatcactaaa gcaaagaaag ataagccaaa tattgctaaa
    38641 ctaattaaaa taaggggatt tagtaatatt cgaaaagcaa gaaaataggg cggtatcttt
    38701 cggtcagcac ttgctttata acccaaatct cctaatttat cggcaagctt ttctttcgtc
    38761 aaggagcctg acaaaaggag ataactattt agcggatcac tacgttcacg actttcttgg
    38821 ctagcttctt ggaattcttt tggtaaagtt ccctgaccat aagttgcata agtaaagtga
    38881 gtcgtcccat ccttactcgg ctctacaatt cttctagcta ttaaactctg ttctgagttt
    38941 gcaaaattct ccaattcctg ttcaaatacc tcacgcgtcg gttcctgagt atcttttttg
    39001 acacgaagta aagaaacgga atcatagctt gcatataaat attgtggcgc acgtaagaca
    39061 ataatccaag caaggaagaa gctgagaaaa aaagttgata ataatatgaa tagtttcttc
    39121 atagtagact ccttgtaaac aaaattcccc ctgtaatttc ttacaagggg aacgatttaa
    39181 atcaatgaac gattagtcat aatcacagta aaatgctact tgttctcccc atttagtcca
    39241 aatccatgca taggaggttt ttccagcaga agcttctcct ttatctgaat gactattcca
    39301 tctacttaca agagaagaat aatgatattt tttaccatga tagtaatttg aaaaagccca
    39361 accacctcct gaaccttctc catacgtcca tactcctcca tctggatatt gtacagcagc
    39421 tgatgcagct cccaataatg taaaacttga aataagagct agagcaagta atctatgttt
    39481 tttcgttttc attttatttt ttctttcaaa aaaagcacac cttgagcaac aatgcaacaa
    39541 aataaaccct cctctctctt ttgttgaaac cgctttctta tgtgataaaa atatcatttt
    39601 tattatttgt tgtcaaggaa aaaatcgaat tttttagata ttttactaca ttgcctctat
    39661 gaataatatt atatagtagt tttattttaa aataatatgc aaccagtact aaccaaatat
    39721 aaaatagatg ccattaacga attttattca agtttttccc attcatacta tccaagcaaa
    39781 agagatgatg ttatagtaca aaacaattca aactattgta aaattcctag caaaaaagag
    39841 agccaaaact ctctttttta tcttctttta ctttttttga ctggcatgag tgtaatgtct
    39901 ctaacactaa agtaagctag gatcaacatg gctattgcta ggaatatttc tgttggtaat
    39961 tgaaaaattt tcagaaaaga tagaaccaat aaaatcaaga gtgccactaa aatacatacc
    40021 atagcgacga tattgactgt ccctttaatg ctttctggtg tcgcaaatac atagagtagg
    40081 agcagtaaaa ttcctaggac taaatagacc atctttctct ctttctagct cttattcagc
    40141 tgattttttc ttcttgttag ctttctcacg ctctgctttg ttaaggattt gtttacgcaa
    40201 acggatagac tcaggcgtta cttccatgta ctcatcgtcg ttcaagaact caagagactc
    40261 ttcaagtgtc aagatacgag gcgtcttgat aacagctgtt tggtccttag tagctgaacg
    40321 aacgttggtc atttgttttg ccttcgtgat gttaactgtc aagtcatttt cacgagagtt
    40381 ttcaccgatg atcattcctt cataaacctc agtacctggg ttgacaaaga tcgtaccacg
    40441 ttcttcgata gacatgattg agtaagttgt agccttacca gcatcgatag aaacaagggc
    40501 accacggtga cgtccaccaa tttcccctgg aatcaatggc aagtattggt cgaaggtatg
    40561 gttcatgata ccgtaaccac gagtcattga caagaactca gttgagtatc caatcaaacc
    40621 acgcgctgga acaaggaaga ccaaacgagt ttgaccatta ccagttgaaa tcatatccaa
    40681 catttcacct ttacgttcag aaaggctttg gataacagac ccttggtatt cttctggagt
    40741 gtcgatttgt acacgttcaa atggttcaca tttaacaccg tcgatttctt ttacgataac
    40801 ttctggacga gatacttgaa gttcatagcc ctcacgacgc attgtttcga taaggattga
    40861 caagtgcaat tctccacgtc ctgaaacagt ccatttatct ggtgaatcag ttgggtcaac
    40921 acgaagggaa acgtctgttt gcaattctgc ctgcaagcgt tcttccacct tacgagaagt
    40981 tacccattta ccttctttac cagcaaatgg tgagttgttg accaagaaag tcatttgaag
    41041 agttggctca tcgatgtgta ggattggaag agcttctact gcatctgtcg gagtgatggt
    41101 ttcaccgaca aagatgtctt ccatacctga aacggcaatc aagtcacccg ctttggcttc
    41161 ttggatttca cgacgttcca aaccaaagaa accgaagagt tttgtaacac ggaagttttt
    41221 agttgtaccg tcaagtttag aaagggtaac ttggtcccca accttaactg taccacggaa
    41281 gacacgaccg ataccgatac gtccaacgaa gtcattgtag tccaaaagtg acacttggaa
    41341 ctgcaaaggc tcatctgagt tatctactgg agctgggata tggtcgataa tcgtgtcaaa
    41401 gattggtgcc atagtcgctt cttggtcagc tggatcatct gacaatgaag aagttccgtt
    41461 gatcgctgaa gcataaacca ctgggaaatc aagctggtcg tcatctgcac caagctcgat
    41521 gaaaagttcc aagacttcat ccactacttc tgctggacga gctgatggct tatcgatttt
    41581 gttaacaacc acgattggga caaggtcttg ttccaaggct tttttcaata cgaaacgagt
    41641 ttgtggcatg gttccttcat aggcatctac gaccaagaca acaccgtcaa ccattttcat
    41701 gatacgctca acttctccac caaagtccgc gtgtcctggt gtgtccataa tgttgatacg
    41761 agttccgttg taagcaacgg cagtattttt agcaaggatg gtaatcccac gctctttttc
    41821 gatatcgttt gagtccatag cacgctctgc caattcagta cgtgcatcaa gcgtttctga
    41881 ttgtttcaat aattcgtcaa ccagggttgt tttaccgtgg tcaacgtggg cgataatcgc
    41941 aatgttacgg atatcttctc ttaattttgt catgatttcc tctataatat tcaaaattta
    42001 ttttctaact gaacgattat accataattt caaataaata acataactca agcaagtgta
    42061 aatgttttca ctccgctttt cttttcacgt caagcctttt caaagcaagc gacttatgat
    42121 aagataggca cagtatgcgt ttagataatt tattagccca agaaaaagtt agccgaaagg
    42181 ccatgaagca agcactcctc agaggggaaa ttctagtcga tggttgccca gcccgatccc
    42241 tagctcaaaa tatcgataca ggactacaag aactcctttt tcaggatcga atcattcaag
    42301 gctatgaaca cacctatctt atgcttcata agcctgctgg tgtcgttaca gccaacaaag
    42361 acaaggaact tccgaccgtc atggacctgc ttccatctga catccagtct gacaagctct
    42421 atgccgttgg ccgactggac cgagatacaa cgggactcct cctcttgacc gataacggtc
    42481 ccttgggctt tcagctcctc catccccaat atcatgtcga taagacttac caagtccagg
    42541 ttaatgaact tctaacacct gaccatatcc aagcctttca aaagggaatt gtctttttag
    42601 atggtactgt ctgtaaaccc gcaaaactag agattctatc tgcaagtccc tccctcagtc
    42661 aagcctctat caccatttca gaaggaaaat ttcatcaaat caagaaaatg ttcctctcgg
    42721 ttggtgttaa ggtgactagc ctcaaaagaa tccaatttgg ggacttcaca ttgaacccag
    42781 atttagcaga aagtaactac cgccctttga accaaaaaga gttacaaatc attaaaaact
    42841 atttagagat gagtcgataa aacaaaaaaa gctttaaaac taaagctttt ttcttttatt
    42901 taccgaaaaa ttaaggcgat tgctacaatc cagttaacta cagaaatcac aattcctaag
    42961 atattaagaa tcttttctat tttatagtct aattgtgact ctttttggta tgaaatagcc
    43021 aagaccaatc ctatgatacc caaaatcaag cctacaattg gaaataacaa accaagaata
    43081 atcgacaaga tacccacaaa aagtggattt ttcttctttt ctttcatgtt ctaagaactc
    43141 tttaaatttt atacaaatta attatactat aaaacaatag cttcatccta tcattcgact
    43201 aatttggaaa taaggttagc tagtcttcac tttccctttc caagaatcca agccataaga
    43261 aaggatataa atctcagaaa aaccttgttt tttcaagtaa agagctgcat ttgtaactcg
    43321 ttgcgcacgt tggttctcgt agagaaggac aggtttatct ttacgaaggg ctgcaagact
    43381 agttttcaac tgacttgaag gaatattgcg tgcaccaagg atatgttttc tgtggaattc
    43441 tgctgggtcg cgcaaatcaa tcaattgacc cgtacgaatc aaggcttcaa actcctcatt
    43501 gtccacaatt ttagccgcac gacgaatacg aagatagtta aagcccatcc acgccaacat
    43561 tgctagtata agtgcccaca aaatccaagt aaccattagt tcttttctcc atttttctca
    43621 atataatcca attctacctt gtgctctctg cgaagaactg cttctgcctc tagatagtct
    43681 aatttatcca tcaaccctgc atcgtaaatc cgagatagtt ccaacttcat cagttcaata
    43741 tcatataagc gttttcccat gtaaacaata ataccaaatc gtttgaggaa ttgctgcaca
    43801 tcatagaatg ttttcataag actcattcta gcaaaatttt gtgttttttt caagaagaga
    43861 ctcacacaat gctccttatt ttcctatctt ctttggcgat tctaaggcaa gtgtggtaca
    43921 ataaaaacat gagaattcaa caattacatt atattatcaa aatcgtcgaa actggctcta
    43981 tgaatgaggc agccaagcaa ctctttatca ctcagccaag tctttccaat gctgtgagag
    44041 atttggaaaa tgaaatgggc attgagatct ttatccgcaa tcccaaggga atcaccttga
    44101 cccgtgatgg gatggagttt ctctcttatg cccgtcaggt tgtcgagcaa actcagcttc
    44161 tggaggaacg ctataaaaat cctgtcgccc accgcgaact ctttagcgtt tcgtctcaac
    44221 actatgcctt tgtggtcaat gcctttgtct ctttgctcaa gaaaagcgat atggagaaat
    44281 acgagctttt ccttcgtgaa actcggactt gggagattat cgacgacgtc aagaacttcc
    44341 gcagtgaggt cggggtcctc ttcttaaaca gttacaaccg tgatgtttta accaagatgc
    44401 tggatgacaa tcacctacta gcccaccatc tcttcacagc gcaaccgcat atctttgtca
    44461 gcaagaccaa ccctctggca aagaaagaca aggtgaaact gtctgatttg gagaatttcc
    44521 cttacctcag ctatgaccaa gggacgcaca actccttcta cttttcagaa gagattcttt
    44581 ctcaagaaca ccacaagaaa tccattgtgg tcagtgaccg tgccaccctc tttaatctct
    44641 tgattggttt ggatggttat accattgcga cagggatttt gaacagcaac ctaaacggag
    44701 acaatatcgt ttctatccca ctggatattg atgacccgat tgagctggtc tatatccagc
    44761 atgagaaaac cagcctatct aagatgggcg aacgctttat cgactatcta ctagaagaag
    44821 ttcagtttga tagttgagaa atgataagaa ccaatatgta ggctagcaac aacctgcaca
    44881 ttggttcttt ttacttataa ttaaaagttt cccctgccaa cttatcagct agcttgggaa
    44941 agagagtata aaacttatgg gctaggttca acaaaatcgg gagattgagt tctcgtttat
    45001 tttttcctat aatcttgaca atctttttag ccactgcatc tgcctctaag aggaagcggt
    45061 caaccgattt aagataagtt ccatctgggt cagcttggtc aaaaaatcct gttcggattg
    45121 gacctggatt gactgttgtc acatagactc catagggcat aagttcgagt cgcagagcat
    45181 ttgaaaaacc aatagccgca aacttggtcg ctgagtaaag actagacttg ccagtagcta
    45241 ttagacctgc catgctgacg atgttgatga tatgcccttt gctgctttcc ttcatacgag
    45301 ccgcaaggtg acgagacaga ttcatcaggg caaaggtatt gacctcaaac atctggtgaa
    45361 tatctttatc agcaatctgg tcaaatccct caaaaatccc gtaaccagcg ttgttaatca
    45421 agacatcaat cttgccatag cggagataaa gatcagttac cagagcttct agggctgaat
    45481 cgtcggtaat atcaatttca atcaattctg catgggaata atttccgtag agttgggcta
    45541 atttttcctt atttctacca agcaagatga gttggtcatt gggcaggagt ttgaccattt
    45601 cttgagctag accaccgcta gctccggtaa tgagaatagt aggcatactt atcctttctg
    45661 tgactgctag atttccactt cttccaagtc tttgaccaca tggacatttt caaaaattgt
    45721 ggcagcgtcc ttcttgagtt tgcttatatc ttttgagagg aaacgggcac tgatatggtt
    45781 gagtaggagg cgtttggcac ctgcttctac cgctacttgt gcagcttgca tattagttga
    45841 gtgaccatgg ttacgagcaa ttttttcatc acccttgcca taagtggact catgaactag
    45901 gacatctgca ttgacagcca gacgcacact ggcatccgtt tttcgagtgt ctcctaaaat
    45961 agtgataatc ttacctggac gtggcgctga gatatagtct gctgccttga tttcagttcc
    46021 gtcttccaaa acaagatcct ggccgttttt gattttacca aaaagcgggc cgaacggaac
    46081 accagcagcc ttgagttttt cagcatccag cgtcccttct agatcctttt gcatgacacg
    46141 atagccaaca cagaaaatag tgtggtccag ctcctctgca tacacagtga atttatcggt
    46201 ttcaagaatt ttacccagag aatcttggtc aaactcatgg aaatgaatgc ggtagggcag
    46261 acgagaacct gacacacgaa ggctggttaa gacaaatgac ttgattcctt gaggtccgta
    46321 gatttccaaa tctgtctgct cttcattggc ctgaaaggca cggctagaaa ggaaacctgg
    46381 caaaccaaaa atgtggtctc catgcagatg agtaataaag attttgctga ccttacgtgg
    46441 tcgaattgtg gtttccagaa tgcgattttg cgtaccttct ccacagtcaa agagccaaac
    46501 ttcgttaatc tcgtccaaaa gtttcagggc gagacttgaa acgttgcggg ctttagaggg
    46561 ctgaccagcc cccgttccta aaaattgaat atccattcga tactttctaa ttaatcaata
    46621 tataacatgg ctgtgcggtt ttccgatcgg aaatagcgtt tgccagaaaa agcagcagct
    46681 tcttgcaata aatcctcttg gctgtagcct ttgagacgtt ttcgaccatc agccaatctt
    46741 tccaaatcag tcaaagctgt gagactttct aggctgacaa cttcctcgtc cttgacaggc
    46801 ttcatgtaaa tcttaccaga ctcttcaaag actaattgat gggagaaaat ttgcgcaatt
    46861 tcaaagagca agtcatccga gattttctcc tcattttcag agaaaatccg accaaagccg
    46921 tcactctcat aacaaaaacc aaaggattta ccagacagat taagccgaat aaaaggctta
    46981 ttttctaggg tgaaacttgg ctcagtattg taaagattca gttcctgact gagttctgca
    47041 aaataatccg tcgcagcctg aggacttttt ttctgataga gttctgcaaa gtaggcatta
    47101 acaacacttg gcggaggtgt aataagtgtt aactgctcct gctctgtttt accagctaga
    47161 agctgatcca gatagacctt gtccagactt gtataacctc catactttag agccaaagtt
    47221 ttaatatcag tcataaaatt cttctaacct ccatttattt ttctcggaaa tgtagcctgt
    47281 aatcacttcg ccgtcttcct gataatcacg ttcttccaga attgcaacac tctctaaatc
    47341 atgaatcttg taggactttg aaaaaggcac tcgcagggta aatgcttcaa aaatttcctt
    47401 aatcttatct agcaataatg cttgcaagtt ttcacgactg tcctcagact tggcagaaat
    47461 gagggtatat ggcgtttggg taggcgtgaa atcctccacc aaatccgctt tattataaag
    47521 cgtcaagtga ggaatatctt ccatgtccag gtctttcatg atggagagaa ccgttttttc
    47581 atgctcctcg tggtaaggat tgctagcatc gataacatga accagaaggt ccacatgctt
    47641 gctttcttcc aaggttgact tgaaactgga caccaactct gtcggcaaat cttggataaa
    47701 gccaacagta tctgtcaaag ttacttggag attgcctccc agatgaatac tcttggttgt
    47761 cgcatccaga gtcgcaaaga gctcatctgc ttcatactgg gtcttactgg tcaagatgtt
    47821 catgatagtt gatttcccag cattagtata accaatcaaa ccaatcttaa aagtgctaga
    47881 ctccaaacgt ttttctctga cagtcgcacg atttttctca accaccttaa gctggcgctc
    47941 gatatccgtg atttgattgc gaacgctacg acggttcagc tccagttggc tttcaccagg
    48001 accacgggaa ccaattcccc ctgcctgacg gctgagcata atcccctgac caaccaagcg
    48061 aggcaagagg tatttgagtt gggctaggtg gacttggagc ttcccttcat ggcttcgagc
    48121 ccgcatggca aagatatcca aaatcaactg catacggtca atgaccttaa caccgagaac
    48181 ttcctctaga ttgacattct gccttggggt cagacggttg ttgacgatga cagtagtgat
    48241 ttcttctgca tccaccataa gcgcaatctc ttccaactta ccagagccga cgaaggtctt
    48301 ggaatcatat ttttcacgtt tttgtctgta gctatctaca acgactgccc ctgccgtttt
    48361 cgctaaacta gccaattctt ccatggagag gtcaaaactg tccataccct gcaattccac
    48421 accaatcagc aggactcgct cctctttttt ctccgtttca atcatctaaa aactcctcta
    48481 tctggcttaa aatgcggtct tgtacaccag attctccaat ctgataaaag gtgacctgca
    48541 tgcgattacg gaaccaggtc agctgacgct tggcaaaacg acgggtcgcc tgtttaagac
    48601 tctcacgagc ttcctccaag gtctgctctc cacggaaata aggaaagagt tccttatagc
    48661 caattccttt agcagcctgt acattagggg aatggtcaaa cagccacttg gcctcatcca
    48721 aaagcccagc ctcaaacatc aaatccactc ggtggttgat acgctcataa agttgactac
    48781 gttcatcatc caagcagata atcagcggtt catacaaaat ctcttgattt tccaaatcct
    48841 gaccaaaatg ggcaatttct aaggcacgca tagcacgacg acgattaaac tggggaatct
    48901 caaggcctgc ttgctccacc aaatgggcta attcctcatc tgaatatggc tccaaactag
    48961 ctcgataagc taaaatctcc tcatgaggag tctccccacc taggtggtaa ccttctagca
    49021 agctctggat ataaagtcca gtcccaccgg cgataatggc tagcttgcca cggctgtgaa
    49081 tatcctcaat agtcatctta gcttctgaaa caaaatcaaa agccgagtaa gactcagtta
    49141 tctctctaac atcgattaaa tgatgaggaa cagctgcctg ctcttctgga ctagccttgg
    49201 ccgtcccaat atcaagtcct cgatagactt gctggctatc tccactaacc acttcgccat
    49261 taaagcactt tgcaacttca atagctagag ccgtctttcc aacagcagtc ggtccaacaa
    49321 tcacaattat ttttgttttc atcttttttc cttgaaaaat tcccattttt tcgttactat
    49381 tattataaca caaaaaggtc agtcagaaaa atgtgacctc ttgaggaggc tggctgattg
    49441 agaatataaa ggaggaagac tcatggctaa aggattcgct aaaggtcttg taacaggtgt
    49501 cgcaggaact gtcgctgccg ttgcaggtgc agtatacgca tttaaaaaga aagtaatcga
    49561 accagaagag caaaaagcag ctttcatcga agaaaaccgt aaaaaagcag ctcgtcgccg
    49621 cgtatcacgt taagaaacaa aagaagttgg gattattctc aacttctttt ttctattctt
    49681 ttaaatagct aggtcaaact tcaactgtgg cttaacaggg tcataatcca ccaactcaaa
    49741 atcttctgct ttgatatcaa agaaattagt cccatcagga acatttaaaa ccaagcgtgg
    49801 ttggcagttt gacggctccc gacggagcaa ttcctgagct tgttcaaatt gattatcata
    49861 gatatggagg ttgttgatga agtagaagaa cttcccaacc ttccagccaa aatgtttagc
    49921 aatcatcatc tgcaaagcca catactgcat agcgttgatg tggtgggcca ccagcatatc
    49981 attggagcgc tgggtcaagg tcgcatccag atagatttcc ccatcaacac gccgaacatc
    50041 aaacatggtc tgaaaggcgc acgggagcag cccatctgtt tcttcgaaag cttggtaatc
    50101 ccagagagaa ataatattgc ggcggttcca aggattggtt tccaactgtt tgagaagctt
    50161 attgataatg tcgtgtttct taacaacggc accatagcgc tcaccaatgg ttcccgtgtc
    50221 tccaacttcc cagtcattcc agtagtgaac attgtacttg tcattaagca cttctaagct
    50281 atttgactgg tcttggtaaa tccagagaac ttctttgatg gcggatttga ttgcaatggg
    50341 acgcaaggtt atgataggaa attctccttt agataagtcg tactcggaaa aggcaccagt
    50401 tacatacttg gaattagcaa cagtcccatc cttgtactta ggacgagcct gctctgagaa
    50461 aacaccttcc tttagaatgc gttcaatatt ctctttaaaa atcgtatctg cttttgtcat
    50521 ttactctcac ctcattcatt gtcctaacta gtatagcata aaaaataaaa gaggaacatt
    50581 actaactcta ggttagcatt ttcctctctc attatcttat tgcaatacaa gtgatgctgc
    50641 tccgataact ccagcgtcat ttcctagagt tgcaagagct aatttagtag atgtgcgtac
    50701 ttgtgggaaa gtattttcgt catagacttt ttgaacacct tgtagaagga attctcccgc
    50761 agctgacaca ccaccaccga tgacgattgt tgatgggttt aggattgaac caatgttagc
    50821 acaagcgatt cccaagtaac gtgagaagtt acggtaaaca atcaaagcaa ggtcgtctcc
    50881 ttcttttgcg agatcaaaga cagtcttagc agttacttct tctccgttat caatcaagcg
    50941 tttcaaggct gcatcgcctt cgtattcatc ggcatagcga cgagtcaagt tgacaattcc
    51001 tgttgcagaa gcgactgtct caagacagcc tttcttaccg caagtacatg agattggctg
    51061 gtcaaagtca acagtgatgt gacccaactc acctgctgca ccagcaacac cgtgaagcaa
    51121 tttgccttct gcgacgatac cgccaccaac accagtaccg agtgtcataa agacaacatc
    51181 tggttggtta tcaccagcac ccatccagcg ctcaccaaga gctgctacgt tggcatcatt
    51241 atcgatgaaa aatggaatgc ccaaagcttt ttcaatcttt tgtttgattg gttgaagggt
    51301 tttccagttc aagttgtagg caccgataac agtccctttg tcacggtcaa ccacacctgg
    51361 tgatcccatt ccaatgcctt ggaagtccgc tgctgccaat ccaagcaagt ccaaacgatg
    51421 ttgaatagac tcaatcatat catcaacgat atgacttccc tcatccaaaa tgttggtctt
    51481 gattgaccat tttccttgga tttctcctgc tgttgttaag attgcaaatt tgatagaagt
    51541 tccaccaagg tcaatcccaa taatcttttg actcattata ttctcctttt tcattgtgta
    51601 gtaattgaaa atgcttacag tactagtata tcaaaatcca ttcacttatg caaaggtttg
    51661 cgtaaatgaa ttataaggaa ttttttcgcc taccagtatc tgttaaccat ctaaatgatc
    51721 aatggcatat tgagctgcat catttgaaaa tttatcaaaa tcagaagtta attgtctata
    51781 catacgtttt tttgacatgt ggaataacga attataactt tttgcctttc ctaaagcagc
    51841 agaatattca ctattgctat cattactaga agaagctgta cttgcttgac cttccttcca
    51901 aactccgtca gcgccaacat aataacgtcc accatcaacc cattcattga cagccatttt
    51961 accatccgat tttagatagt aatttccttg ccatgtacta taagcataag aaccatctga
    52021 tgtcaaataa taccaagctt gataggtggc atcataaacc cattcacctt tagccatttt
    52081 accatctgat ttcaaatagt agtttccttg ccatgcattg cgagcatatg aaccatctga
    52141 tttcaagtaa taccatgctt ggtaagaaga atcataaacc cactcacctt gtgccatttt
    52201 accgtttgat ttaaggtaat aagctccttg ccatgcattt tttgcataag aaccatctga
    52261 tttcaaataa taccaagctt gataagaaga gtcataaatc cattcactct gtgccatttt
    52321 gccatcagct ttcaggtaat agtttccctg ccatgcattt ttcactagat ttccatttcc
    52381 atcaaaatag taccaagaac cgtcctgctt aacccaacca cttgctgtgg atgaagttgt
    52441 atcagtttga gttgtagccg cttgttttgc tttaaaagca cccttaggag catttttcaa
    52501 ttcgcaagcc acaccatcat gatcgcggtc taacttggca gaataaccag gttctccctc
    52561 atgaagatcc gaatatccat tagcccaagc ttccttacag ctcgaaaaat ggatgttttc
    52621 ttccgccaaa actggttgtg aaaacaaggc taaaattggc aacgtcacca gactcatttt
    52681 taaaaataga cgtttattca tttctttctc ccataattat gatatcgatt tcaatattat
    52741 tttatcaaac ttatttcagc aattcaagga aacttctggt cttttctaca aactcctttg
    52801 gttttgcctt attcaagaca tgagggccat caggaatgat ctgaaactgc gaatctgtta
    52861 acagtctatg gagagctttc atagaactaa gattcggttt atctttgctg ccacaaatta
    52921 gtaaggttgg ataggagcaa gtccctgcta tatccgttaa atcaagtgtc ttcaattcct
    52981 cagaaactcc gaccataaga gtcttgtctg ctccctgttt ttcaaatact cttttgggaa
    53041 gtagtttaaa aatcagcaac tgaagataaa ataggatatt ccctgctaat ttaagcgggc
    53101 atcctgacag aatcaaagct cgaagatttg gtaaatcgta actggaaagt tctagtgtca
    53161 gggcagcacc taaggacaat ccaatcaaaa caaaaggttc tgtctcttga gctaggtgct
    53221 gataaactcg ctctttagct tgttgatagt tactaactcc agaaggaaat aactcgatag
    53281 cctcagaagg ataatctgtc agtagattcc gaacttcttt ccaagactct gctgactgcc
    53341 ctaacccatg caaaaatatc aatttcatct agttctcctc aaggcttagt tcatacaagc
    53401 ctctcactgc attacagccg taaatagctt ctgcttgggt taaatctgcc aaggtcaaga
    53461 ctttctcttc tacctgtcct gtttctagca aatactgacg gtaaattcct ggtaagattc
    53521 caagtcggat aggcggtgtg tagagttttc cagcgatttt cagaaccaaa tttcctatag
    53581 aggtttcaag cagttctcct gacttattgt ggtaaatctt ctcttgttct cctaggctca
    53641 aatgcggtcg gtgagtggtt ttaaagtagg taaaggattg attcaaagca gcttcctgaa
    53701 ggcagacttg ggcctgacaa aagcttgtac tgagaggggt taatacttga cgattgactt
    53761 ctatctctcc agatttgcta aggctgattc gcaagcggta atctcgatta gcttcacaat
    53821 cctgacactc ttcctcaatc ttgtgtccca agtcttctgc atcaaaagga aaagcaaaat
    53881 aacgactagc ttttctcagt ctttccagat gttgatcttc aaacagcagg ttcttctggc
    53941 tgatttcccc tgtcgtaatc agttggaagc gagcttgttt acgataaaga actgctgcct
    54001 tttgatgaac ctctcggtat tcagattccc atgtgctatc ccaagtaatc cctccgccaa
    54061 ctccataaat ggcttgacct ttgtgaagtt gaatggtacg aatggccaca ttaaaaatcc
    54121 gtcgtccatt tggaagcaag agaccaatcg ttccacagta gactccacgc ggttgaggct
    54181 ccaagtcctt gataatctcc attgtcgcaa ttttcggtgc acccgttatg gaaccacaag
    54241 gaaagagtga gcggaagatt tcaacaaggt ccacatcctc tcgcaactga ctcttgatgg
    54301 tcgaagtcat ctgccaaaca gttgaatact gctctacctg acacagacgc tccacgtgct
    54361 cgctcccaac ttcagaaata cggttcatat cattgcgcaa gaggtccaca atcatcatat
    54421 tttcagagcg atttttggga tcctgttcca accaactggc ctgttcaaga tcttcttggt
    54481 cagttacccc acgctgagtc gtccccttca ttggtcgtgt tgtcaactcg cgatcatttt
    54541 gctcaaaaaa gagctctggg ctcatggaaa tcactgccat ctcgtcatgt tccacatagg
    54601 cattgtagcc cgcctcctgc tctaccacca tacgattgta gatggcaaaa ggattggcac
    54661 ttaacttttg cttgagttgg acggtgtagt tgacctgata ggtgtctccc tgcctcaagt
    54721 gatggtgtat ctgggcaatg gccttttcat agtctgctgc agacgtcact tcctgccaat
    54781 ttgagggcaa atcaatatcc tcataagtca gaggaatagg ggaagtttct acgctatcat
    54841 gaacagtaaa gtaaagcagg tactctccca gtaggggatc cttgtgaact gctaattttt
    54901 cctcaaaagc aggtgcagcc tcgtagctga cataccccac cacataataa ccttgctctt
    54961 ggtagctttc cacttgtgcc agcaaatctg ccacttcttc tacatttctc gttttcaact
    55021 ctttaatagg ctgggcaaag gtatatctct cccctaaagt cctaaaatca atcactgttt
    55081 ttctatgcat accttaagta tagcataaaa aggcagatac agacaaagct gatctacctt
    55141 tttacgattt tattcattct atgcttgtgg tgcagtaaat agatcaccag agaaaccgtc
    55201 tgtgttttta aggagagctt taaagacttt cgatttcaag acaactgtat ttcggtattt
    55261 gtctttaata ctactatcgt ttagttccgt taaatcttta gtaacagcct catcaaacat
    55321 agtcttcaac tgttcatagg ttgaaatttg agtgccatta atttcaattg gtgcaaaacc
    55381 ttttttagat tttgtaagaa cttccccata ccattgcttc ttccaatctt caaggtttga
    55441 aaattggtta ttagaaactt tcttaataat gaaatcatca cctaatagac cgttattttc
    55501 tttattggct gcaccttgat gtttgcttga aacatagtca atgaacccat gttcatatcc
    55561 atagtaaccc cacatacgga aggcgttatg tttgaaggat agagaaccag gggcaccttg
    55621 actcgtatta ccgccataaa taccagccat catattaaca ttgacatatg ccgtaccaaa
    55681 gtcctctggg tggaagacct tattacctgg atttccgtga atggtcatga aattattatt
    55741 taccaagtca tcgatggttt caaggttagt tgttttctca gcgtctatca aatcacgcac
    55801 tttgtcccat tggtgaggac cacttagttt atttctgtcc attggtcgac gtgtttcgcg
    55861 atcaattttc ttgaaccact tactattgtc acctttaacc ttaggaagaa cagcctcagc
    55921 ttctgcataa tcgagcatca tcatagcttc attgtagttc ttcatgtaat ggtcgatttg
    55981 gtcacgtgtt tgaagtttgg taggatctgg attataccat tggtttccat catttgaacg
    56041 ttcaaatgcc atatttaatc caagtgcgcc atattcacct tgatgtccat gttctgctgg
    56101 agtttgtaac ataccttgag caaatgcttc aagatctgta ccctgtcgat gtctatgacc
    56161 tcccagatat gccattcggt catttacgtg ggtagtctca tgtgtaaagg cagatgtgcc
    56221 atattcactt attaaatccg acataacaaa atatacagca tccataggct gtggatcagc
    56281 aaagatgtaa gcatacgctc catacccata cttataatca tgatacttat caatacgtcc
    56341 aaagaattca cgaataggtg cataatccgg tttatctttt cgatgaccaa atctatcagg
    56401 ccaaccaccg ggtgcaccat gattatccca tacaggagtt ggaactacat tttgactctt
    56461 aagaagttta tcgcggacat tatcagtggc aaggcgtgac caaaaatcta agtaattaac
    56521 ttgctcttta gcacgtttat tgatttcact cttgaacgat tcacgttttt cttctgtatc
    56581 tttaccatac ttttcaaacg aactaaatga gatagtatta tatgttgaaa tcataaataa
    56641 atgtgcatct ttcaagttca gaagcgataa aatcatgcgt ccatgttgtc cgttattaag
    56701 tccttcatat agacgatatt ttttattagc aaatgcaggg ttagttgacg gttgttcaac
    56761 tacataagca ttattttcaa tagctttctt aaaccaaaca tttatgtctg tttcatttgt
    56821 gaatagtttc atattgtagg tcaagaagtc atgtaagttt ccagaaagag tatttttcgc
    56881 caccacttca cggaaggact cctgtgtacg acttccctta atatagtcct cttttgaacc
    56941 aatttctatt aatcgatcta aaacacttac attcttacca taaaaatctg gtttgaatag
    57001 cattaattct ttaatattga agttatcaaa tttcacaccg taataacgat ttaggtatgt
    57061 taatcctaac aggacagcag ctttgttatc ttcaattttc ttaatcaagg cacgttttgc
    57121 agcttcatct gtattcagtt gatgatcttc attttcaact aattttgtca caaatgtatt
    57181 aagatgatct tttacttctt taaagctttc ttctaagtaa agattcttaa ttgcattaac
    57241 tctgttatcc ccagttcttc ctaatcgttg ataaattgga tcagattgta attcaattgg
    57301 acttaaaata ccttcaatag cactaattaa atctgcacga tctttaacaa ccatatttgg
    57361 tgtatagacg acttcaccaa gctctgcgac actgtattct tttacttgct taacttgaga
    57421 gtctttagga gaaatcgtaa agatatcttt tgtcttatct gcgtaatgaa ccataatatg
    57481 gtcagcatct gtaaggtctg taataaagtc attacctttc attgcagtca cagacaatac
    57541 ttctttattc attaatggac tatcagttgt aagcttgttc ccttgattca caatccattc
    57601 tttgttgtag aacggttgga gtttttcaac attacgataa acaagctcac gatttgcatt
    57661 ataatcttga attgttttgt attcttcatc acgtaatata cggcgattca acttatcaac
    57721 caaatgctca gttacttcaa atgtatcagc agtgataccc atatccttaa ttttcttatc
    57781 agcttctacc tgtgaaattt ctttaatttt agacgattgt ttatgggttt tatctccaga
    57841 actcacaccg ataactgttg cattattagg tttaatccaa ttgcttacaa attcatcatc
    57901 catatcatta gaaccaaaga aaatatcacc acgcttaaca ttcactaagc tgatattgtt
    57961 atcaatggtt ccccaaggcc agtctttacc aattaaaccg cctacatcac ctctagcaac
    58021 atcaatagta cctttggcta cagaatttct tacattagac caagcaccaa catctcttgg
    58081 attaaaatca tgagctaaat gtcctactat agcacctgca cgagtattta aacctttaag
    58141 ttcaacatct gcataagctt tatttaaatt gcctcgataa atctcagatg caatcccagc
    58201 caagttccac cctttatcac caacagattg aacttttccg ataaaagcaa cattagtcat
    58261 aatacctgca tcatctaatt tatccaaaat acccgcaaca ttgttattac caacgatagt
    58321 acccgttact ttaatatttt caacagttgc atttttcact gtttgagcta atgaagctaa
    58381 ttgatttgcc caaggcatat taatattaac gttaattaaa ttaatatttt tgatagaacc
    58441 cccttcaact ctatcaaata atggacgagc agtgttatga atggagaaat gcttaccatc
    58501 aacacttgtt aagtgacctg taaatttacc tgttacatat gatttacttg gtgtaggtac
    58561 atttgttgca tttaaatccg atcctaattt aaactcaccg ttaggatttt tctgcatagc
    58621 atctactaaa tctttgaagt tatagtaaac gttattttgc gatgctttgg gtttctctat
    58681 gtaataagtg tatgtttgac tcaaactatt atttgaatca cgttggatta aatcaggagc
    58741 tgaagcagta actttaaata aggatgtctc ctcctcagtt acttcctcta tattagtaat
    58801 ggcaagcctt gttactttat tatgattagt ggtaattttt agataatatg acgttagatt
    58861 ttctggaaca gtttctagaa ggctactatc tgtttcattt ccattctcat ccactttcat
    58921 caatcttgtt tctttaatat ctttaatctc aactttttta aggtctaact gaacttcttt
    58981 atcttctaat atttcagttt cttcaccgtt acctcgatca tagaccatgg ttgtggatag
    59041 tgtataacct tgataatatt tcaagttgtc taaagtagct tgtaagttat tttcttctac
    59101 cgttacttct ttaacaactg ttccagtatt atcttttaga gctactttaa tcgatttaat
    59161 ttctactcca gctggtttag tcaatgtata actagctgtc gcactcttct tcaagacatc
    59221 tttatctgtt ttagcccatt ccaaggttgg tttctctaac tcttttgtgc cattcttaat
    59281 aattttgtct tgaggttttt caatcactgt ttctgtgata gtaggagcgt cacttgtttt
    59341 ttctccgcga attgttttat aggtttcagt aatttgtttc ttatcgttta caccttcttg
    59401 agttacttgt cttgttccgc gttttaaagt gtcatcttct tgttcttttg tttcaaaagg
    59461 aatgatttct tctcttgtct caaccgttgt cccttcaatt ggttttgtac cacggattac
    59521 ctgttgattt acaggctcct tggtgacttc cgtactggtt gaaagaactt ggtcagtttt
    59581 aacccctcct attgttttat aggttgtttt tgtaacttga cttccttttt caccatttcg
    59641 taggacagtt tcttcgtccg tgtacttggt tgggtcctca gtcgtttcag ttttatagtc
    59701 aagttctgta gttgattctt caacgagtgt tccttcagtc accttatact caggtaattg
    59761 ttctggaaca agagcctcac ccacgtaatc ttcttcttgc gtccccttag ctgttttagt
    59821 ctcctctttt ggttctacag tttcttcagc cttggtatct ggagttgact cttcttgttt
    59881 cggtgtctct tccgctcccc tatcttcttt aggattgtct tcttgtgatg attcttttgg
    59941 tgtacttggt tgctcagctt gtgtaggttg cacgggttgt tctgggactt ttgcttgttc
    60001 tactggtggt tcaacttttg attcctcagc tggtttgtct gatggttgac tttctggctt
    60061 aactgctact ttttcctctg attttgactc aacttctcca cctacttctt caactggagc
    60121 tggttctgct gaatcttctt tcccctcttc tactttagga agggtgtcgt cagtaggttt
    60181 tacctccgat tttggttctt cctttggact ttcttctgtt ctaggtgctt cttcttttgg
    60241 agtttcctct gtctctacta cttggttttc tgtcctagct tgctcctgat ttgttgttga
    60301 ttgaggagtc tcaacttcga ctacagtcac ctctccaggt tttgctgagg tttcttctaa
    60361 aacagtgtcc aagccaagcg ttttgaggat gtcacctgat agataaccaa catagcgata
    60421 gccatccatt tcaacaacac cctctcgact agccagcgct aaggtcgcaa ctgggtctac
    60481 agcccctgca ctaggaagaa ctaccaatcc catagctcca actagaaaga cgctagcaat
    60541 tttctttctc ttgtagatta aaagcaagct cccaacagtc agcaaaccaa aagctgtcaa
    60601 aacagatgct tctgtccctg tttgaggcaa ctgatctttt tgatacacca aaccatatac
    60661 aacttcattc ctgtcaggct ttcctgtctg aattaaatct ttagcttctt gtgaaataat
    60721 ctctttattt acatagtgat aggtagctgc gtccactaca gaaggagcca tcaaaaggct
    60781 tccaagaaat acagagccta caactccctt aatcttacga attgaaaaac ggtctttttt
    60841 aaacactttc atctccttta ttcattctca agactttcta atagcatctt gcggatagtg
    60901 cgcacgcgca cctccgatta attttggacg actagccagt gccgttacat gggcatgacc
    60961 aatctctctc aaaatagggc gaatcggaac ctgaacatgc ttgacatgca tgccaattgc
    61021 agtgtctccg atatccaatc cagcatgagc cttgataaat tcaacctcaa ctggatcctg
    61081 cataaactta aaggctgcca actgccccga acctcctgca tgaagagtag gatggacact
    61141 gacaatttcc agatcaaact gctctgccac ctgacgttca acaacgagag cccgattgac
    61201 atgctcacaa ccttgaactg ctaaatggat acctctacta cctagaatac ccaagatagt
    61261 ctccactatc agctcaccaa tctcttgact ggattctttc ccaatctgac cacctagcac
    61321 ctcactagaa gatagaccta aaacaaaaag gaccccctgc ttcaaattgg tcttttctaa
    61381 aacatcttcc actacctgac gtgtttctct ttgaatctgt gtctcgttca tctctgttac
    61441 ctctgttgtc actcttctat cataccgttt tttcttgttt ttagcaagat agacaaccta
    61501 taaagtctgc ccaattacgc ataaaactcc cagaattgac tgggagttag ctagtttcta
    61561 ttctatttat atatatttca actttcgttc ctttttgggg tctagaatca atcttcatct
    61621 ggtaattggc tccaaaatga agtttgagcc gttgatcgac attttgaaga ccaactcccc
    61681 cacgtttgag ttgactttga ctactatcac cagcatcttg gaagccaacg ccatcatcct
    61741 caatacggat gaccaatccc gaatcctgtt tctggacaga aagtttaata tggccctgac
    61801 cttccttttc cttaatgcca tggtaaagag cattttctac aaggggttgt aggaccagct
    61861 tgggtaagac taaattatca aaggcaacat tttcattaat ttcgtattcc agcttatctc
    61921 catagcgttg tttctggata aagagatact ggcggacatg attgatttcg tcagagagac
    61981 aaatcaagtc cttgccttga ttgagcgcca agcggaaata ggttgccaag gacttggtca
    62041 cctgcaccac tcgctgacta tcatgaaatt cagccatcca gatgatggtg tccaaagtgt
    62101 tatagaggaa atgtggatta atctggctcg aaagggcttg aagttggtac tgacgggtcg
    62161 tttcttcctg gctacgaata gctaccatca actgatcaat ctgatccaac atagcattaa
    62221 attggcgagt tacttctctc agttcatagg caccaacttc cttggcacga agattttgag
    62281 caccagaagc aatttccaac atggtttctc tcaaatcctt caaaggagca atccagcgtt
    62341 taagactgaa ccacactaag cagagacaaa caagaagaga tgtgacactg gccccaagca
    62401 aggtccacaa gagctgactc cgaacctggt ctaacttttc caatgatgac acgccaagta
    62461 ccgtccaatc agttcctgca atcttctctt gactgacgta ggatttgtga ccaggagtat
    62521 aaccctgacc tgtctcgatg tagggtttca tagcctccat tttgctagac gaactataaa
    62581 ctgtgtgttg aggatggtag acaaattcat ggttttcatt gataatgaag gcaaagccct
    62641 gctgccccaa ctggagttga ttgagatagg cttccagagt ttcataagaa atatccaaac
    62701 gaagcacacc aagattggct ccctttgcat caacaagttc ttgagtgaca gaaatgaccc
    62761 actgactatc tgatttacga gctggagtca aaacaggcat agctccctga tgaatagcct
    62821 tttggtacca atcctcagcc atcatatcag aggaagtttt catctgcaca ctgtcatctg
    62881 tagaaatgac ctgaccagat ttggtcacca gcacaacagt tttcaagtcc ttatctgact
    62941 tcaagatggt caaaaacaaa tctcggattc cctcgacctt gtcttgactg ggattctcag
    63001 cataggccag aacatccgtc tgctgggtca aaccagtcga ggtggtttct agttttttga
    63061 tataagactg aataaagtgg ctagtctggc tgatggtcgt ttggctgttg ccctcaatgg
    63121 tggcctcaat ggctgaagaa cttgattgat agtagaaagt tccaaccaga gctaggagaa
    63181 tgagaaagac cagaaagatg gaaataacca ttctaactaa aagagaagaa cgcttcatcg
    63241 gtcttctccc ttcttaaact gacgaggtgt cacacctgca atctgcttaa aacgttgggt
    63301 aaaatagttc atatcttcaa aaccaacctt ctctgcgatc tcataaatct tcagatctgt
    63361 agttaaaagc aagagcttgg cttgtttaac acgttctctc accagataat cctgaaaagg
    63421 caagcccaac tctttcttaa tcaaggaact cagataggtc ggactaaaac ctaagtcact
    63481 ggctaaagac tttaaactaa attggctatc agccagatga gactggattt tctgggccat
    63541 gtttccttca aacctattag tcaataaatc ttgtaactgc tcttctttct cttccttgtc
    63601 tagtttttgt ttgattttcc ccaacatttc ctcaatatcc tgacgagaaa agggtttgag
    63661 caggtagtcg tccacaccta gtttgacagc agacaaggca taatcaaaat catcgtaacc
    63721 tgttaaaaag accaaatgaa cctgaggata ggtttctcgt accagactgg ccaactggat
    63781 gccatttaga tgaggcatgt tgatatcggt taaaatgata tctggcacct gcttttggat
    63841 caattcccaa gcctgccttc cattttcagc ctgaccgatg atttccatat cgtaggctgc
    63901 tacattgacc agtttagtca aaccttgtct taccagatat tcatcttcta cgattaagat
    63961 tgtgtaggtc atgctctgct cctttaccac ttactagtat cagtatagca aaattctcct
    64021 ctaactgctt aggaaagacc tcttatactc aataaaaatc aaaaagtaaa ctaggaagat
    64081 agccacaggt ttctcaaagt atagctttga ggttgtaaat aaaactgacg aagtcagatc
    64141 aaagtatagc tttgaggttg ta
//
LOCUS       DAVBBS010000005        51676 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVBBS010000005 DAVBBS010000000
VERSION     DAVBBS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 51676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51676
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.005"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(54..362)
                     /locus_tag="VQU32_000298"
                     /pseudo
     CDS             complement(54..362)
                     /locus_tag="VQU32_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            complement(604..900)
                     /locus_tag="VQU32_000299"
     CDS             complement(604..900)
                     /locus_tag="VQU32_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471821.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYSKMK"
     gene            complement(884..3043)
                     /gene="pfbA"
                     /locus_tag="VQU32_000300"
     CDS             complement(884..3043)
                     /gene="pfbA"
                     /locus_tag="VQU32_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HET2471822.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVISTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKESVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            complement(4173..5399)
                     /locus_tag="VQU32_000301"
     CDS             complement(4173..5399)
                     /locus_tag="VQU32_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HET2471823.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVFEVGPGDEVIVPAITYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(5442..6134)
                     /locus_tag="VQU32_000302"
     CDS             complement(5442..6134)
                     /locus_tag="VQU32_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET2471824.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGNLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSTEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFKVLK"
     gene            complement(6320..8071)
                     /locus_tag="VQU32_000303"
     CDS             complement(6320..8071)
                     /locus_tag="VQU32_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2471825.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYRLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALVDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(8061..9806)
                     /locus_tag="VQU32_000304"
     CDS             complement(8061..9806)
                     /locus_tag="VQU32_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2471826.1"
                     /translation="MSIIQKLWWFFKLEKHRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSPAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTELRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSEVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILNPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGRHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(10012..10308)
                     /locus_tag="VQU32_000305"
     CDS             complement(10012..10308)
                     /locus_tag="VQU32_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HET2471827.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(10461..10917)
                     /locus_tag="VQU32_000306"
                     /pseudo
     CDS             complement(10461..10917)
                     /locus_tag="VQU32_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            11051..11878
                     /gene="xth"
                     /locus_tag="VQU32_000307"
     CDS             11051..11878
                     /gene="xth"
                     /locus_tag="VQU32_000307"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET2471828.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            11890..12033
                     /locus_tag="VQU32_000308"
     CDS             11890..12033
                     /locus_tag="VQU32_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET2471829.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEYQRSEENFLFLLK
                     SVN"
     gene            12033..12272
                     /locus_tag="VQU32_000309"
     CDS             12033..12272
                     /locus_tag="VQU32_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET2471830.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRPLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      12656..12751
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            13151..13732
                     /locus_tag="VQU32_000310"
     CDS             13151..13732
                     /locus_tag="VQU32_000310"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HET2471831.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            13732..14994
                     /locus_tag="VQU32_000311"
     CDS             13732..14994
                     /locus_tag="VQU32_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HET2471832.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LSAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(15081..15947)
                     /locus_tag="VQU32_000312"
     CDS             complement(15081..15947)
                     /locus_tag="VQU32_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HET2471833.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIM
                     NSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFDFSKRNYS
                     IEEYTMFLEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLE
                     AEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFY
                     SGSGSKLNETARSYKMIAEEIKAIPNVEFMWITDGQGWYKAKNNLRETFDILPFLYNI
                     NDLEHNILKNLK"
     gene            complement(15934..16704)
                     /locus_tag="VQU32_000313"
     CDS             complement(15934..16704)
                     /locus_tag="VQU32_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET2471834.1"
                     /translation="MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNG
                     GISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYS
                     VGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
                     KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGS
                     GTTGVVAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
     gene            complement(16730..17584)
                     /locus_tag="VQU32_000314"
     CDS             complement(16730..17584)
                     /locus_tag="VQU32_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HET2471835.1"
                     /translation="MKIKEIKKVTLQPFTKWTGGKRQLLPVIRELMPKTYNRYFEPFV
                     GGGALFFDLAPKDAVINDFNAELINCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRS
                     ADRDERIDMMSEVQRAARILYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELIS
                     AISVYLNNNQLEIKVGDFEKAIVDVRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFAD
                     QVRLRDAFKRLSDTGAYVMLSNSSSALVEELYKDFNIHYVEATRTNGAKSSSRGKISE
                     IIVTNYEK"
     gene            complement(17741..18145)
                     /locus_tag="VQU32_000315"
     CDS             complement(17741..18145)
                     /locus_tag="VQU32_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET2471836.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVRI"
     gene            complement(18215..19696)
                     /locus_tag="VQU32_000316"
     CDS             complement(18215..19696)
                     /locus_tag="VQU32_000316"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HET2471837.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIVYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSADHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFARVLEDAGVYKQTEQGQTAFMRFVEHVGILLD"
     gene            complement(19715..20893)
                     /locus_tag="VQU32_000317"
     CDS             complement(19715..20893)
                     /locus_tag="VQU32_000317"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HET2471838.1"
                     /translation="MAQHLTTEALRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVFENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            21058..22065
                     /gene="galR"
                     /locus_tag="VQU32_000318"
     CDS             21058..22065
                     /gene="galR"
                     /locus_tag="VQU32_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HET2471839.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFRNYSQAKGIYHDELVFQGN
                     FTAQSGYDLMKEAIQNLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHDRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(22111..23148)
                     /locus_tag="VQU32_000319"
     CDS             complement(22111..23148)
                     /locus_tag="VQU32_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET2471840.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAVEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLQSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(23149..23502)
                     /locus_tag="VQU32_000320"
     CDS             complement(23149..23502)
                     /locus_tag="VQU32_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET2471841.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(23746..24636)
                     /locus_tag="VQU32_000321"
     CDS             complement(23746..24636)
                     /locus_tag="VQU32_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET2471842.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            24769..25305
                     /locus_tag="VQU32_000322"
     CDS             24769..25305
                     /locus_tag="VQU32_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET2471843.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(25472..26278)
                     /locus_tag="VQU32_000323"
     CDS             complement(25472..26278)
                     /locus_tag="VQU32_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HET2471844.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(26623..28143)
                     /locus_tag="VQU32_000324"
     CDS             complement(26623..28143)
                     /locus_tag="VQU32_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HET2471845.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPNLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLAALIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLVQKEKENLVIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGKTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMEPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(28136..28864)
                     /locus_tag="VQU32_000325"
     CDS             complement(28136..28864)
                     /locus_tag="VQU32_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2471846.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKAPATDFVADLFGGSVHD"
     gene            complement(28879..29643)
                     /locus_tag="VQU32_000326"
     CDS             complement(28879..29643)
                     /locus_tag="VQU32_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET2471847.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVVDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHDIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(29647..30090)
                     /locus_tag="VQU32_000327"
     CDS             complement(29647..30090)
                     /locus_tag="VQU32_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2471848.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKFQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(30772..31068)
                     /locus_tag="VQU32_000328"
     CDS             complement(30772..31068)
                     /locus_tag="VQU32_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET2471849.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETAKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(31090..32154)
                     /gene="pepA"
                     /locus_tag="VQU32_000329"
     CDS             complement(31090..32154)
                     /gene="pepA"
                     /locus_tag="VQU32_000329"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HET2471850.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            32555..33256
                     /gene="galE"
                     /locus_tag="VQU32_000330"
     CDS             32555..33256
                     /gene="galE"
                     /locus_tag="VQU32_000330"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET2471851.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            33679..34638
                     /locus_tag="VQU32_000331"
     CDS             33679..34638
                     /locus_tag="VQU32_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2471852.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKEISESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLGFILLLWRGGRKDAD"
     gene            34628..35584
                     /locus_tag="VQU32_000332"
     CDS             34628..35584
                     /locus_tag="VQU32_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HET2471853.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLTLQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            35581..36333
                     /locus_tag="VQU32_000333"
     CDS             35581..36333
                     /locus_tag="VQU32_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2471854.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGS
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            36429..37394
                     /locus_tag="VQU32_000334"
     CDS             36429..37394
                     /locus_tag="VQU32_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2471855.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPAILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(37619..37861)
                     /gene="yidD"
                     /locus_tag="VQU32_000335"
     CDS             complement(37619..37861)
                     /gene="yidD"
                     /locus_tag="VQU32_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET2471856.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(37861..38583)
                     /locus_tag="VQU32_000336"
     CDS             complement(37861..38583)
                     /locus_tag="VQU32_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET2471857.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            complement(38570..39139)
                     /locus_tag="VQU32_000337"
     CDS             complement(38570..39139)
                     /locus_tag="VQU32_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HET2471858.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKSQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            complement(39158..39886)
                     /locus_tag="VQU32_000338"
     CDS             complement(39158..39886)
                     /locus_tag="VQU32_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET2471859.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            complement(39886..40626)
                     /gene="xerD"
                     /locus_tag="VQU32_000339"
     CDS             complement(39886..40626)
                     /gene="xerD"
                     /locus_tag="VQU32_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET2471860.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            complement(40617..41078)
                     /locus_tag="VQU32_000340"
     CDS             complement(40617..41078)
                     /locus_tag="VQU32_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2471861.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            complement(41075..41596)
                     /locus_tag="VQU32_000341"
     CDS             complement(41075..41596)
                     /locus_tag="VQU32_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET2471862.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            complement(41572..42543)
                     /locus_tag="VQU32_000342"
     CDS             complement(41572..42543)
                     /locus_tag="VQU32_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HET2471863.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGKSSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            complement(42540..43334)
                     /gene="racE"
                     /locus_tag="VQU32_000343"
     CDS             complement(42540..43334)
                     /gene="racE"
                     /locus_tag="VQU32_000343"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET2471864.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIYVEHVEL"
     gene            complement(43541..43789)
                     /locus_tag="VQU32_000344"
     CDS             complement(43541..43789)
                     /locus_tag="VQU32_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HET2471865.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            complement(43931..45556)
                     /gene="treC"
                     /locus_tag="VQU32_000345"
     CDS             complement(43931..45556)
                     /gene="treC"
                     /locus_tag="VQU32_000345"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HET2471866.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTGNGIGDFRGIIGKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCIGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            complement(45734..47701)
                     /gene="treP"
                     /locus_tag="VQU32_000346"
     CDS             complement(45734..47701)
                     /gene="treP"
                     /locus_tag="VQU32_000346"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HET2471867.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGNDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            47886..48596
                     /gene="treR"
                     /locus_tag="VQU32_000347"
     CDS             47886..48596
                     /gene="treR"
                     /locus_tag="VQU32_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HET2471868.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            complement(48629..49432)
                     /locus_tag="VQU32_000348"
     CDS             complement(48629..49432)
                     /locus_tag="VQU32_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2471869.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKHLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKLKLLLLDEHTAALDPKTSQMVMDLTQKIVEYHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(49445..49873)
                     /locus_tag="VQU32_000349"
     CDS             complement(49445..49873)
                     /locus_tag="VQU32_000349"
                     /inference="COORDINATES: protein motif:HMM:NF014684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471870.1"
                     /translation="MKTEIGLVLRSTGDNIPMSEANGVNVNTMKIVGYMISTGLIALC
                     GSLFAQNDGFSDVTSGTGTIVVGLSSEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYE
                     IPNLDQNLVRLFNAILLALVLFAPALQKRLKIRGLKLRNE"
     gene            complement(49882..50322)
                     /locus_tag="VQU32_000350"
     CDS             complement(49882..50322)
                     /locus_tag="VQU32_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2471871.1"
                     /translation="MNFVLSSLSEGLLWSIMAIGVYLTFRILDIADMTAEGAFPMGAA
                     IVVSQIQAGANPWLATLLALLAGMVAGLISGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGAVFSLSLVCLLLVWF"
     gene            complement(50367..51272)
                     /locus_tag="VQU32_000351"
                     /pseudo
     CDS             complement(50367..51272)
                     /locus_tag="VQU32_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512061.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT        15175 a        11736 c         9477 g        15288 t
ORIGIN      
        1 attaaaaggt taagtaattt ttccaaaata cagcactcat atagacagta tcatcaatta
       61 tagccattgg gattttttga ttgccaattc catgtatctc tacacatgtc ttcaatcgat
      121 tttgttgtcc tccaatttaa ttccttatat gctttgtctg catttgcata ataagttgca
      181 acgtctcctg aacgtcttgg aactatttta taaggaatag ggatcttatt aacactttca
      241 aatgtattta caagttgtaa tacactagtg ccttctcccg agcctaggtt atagatataa
      301 acatctgttt tttcagatac tttttctaaa gcttttatat gtcctattgc taaatctact
      361 acttgaatct tttatctcat ttttcccttt ttcaaataag ttatctactc tatgatcagt
      421 tatttccatt tcgtttacta acatgacgca gtcacctagc atcatatact ccaacttttt
      481 ttctgaaagt ttagcaaata ttggtaagcg ataatatagt atattgaaac tagaatagta
      541 cacctctact tctaaaacat tgttagaaat cgatttgact gtcctgattg atttgtccta
      601 ttattatttc attttactat actctgttaa ttcatatgag tttaaaccga tttcatcttt
      661 aacctcgagt aaagcagttt caaatatttg tttaagagtt tttgattctt tacaattaac
      721 cgacaaactt tctgataaaa tatgtacaac ttctgagact gaataaccta tctcctcttt
      781 agaattatat aaatctgtag ctccaccaat aatccaaaaa tactgatttt gtgaacctac
      841 aatatcctca ttttctacgg aacttccttg tatcgaacaa attttattta tctttaccat
      901 aatacttcaa cccttttagt gtcaaaagta aaccaattcc tgtcactgtt aagaatagtt
      961 ccataatctt gttcgaacca gtctttggta atttttgttt tacatctact atttctttag
     1021 atttattaat atgattttca gtttctctgc catctccaac tattttatag tttacttctt
     1081 ctgtcttatt atcttgttta ttgtcgatct tgtcattcat ttgtctatta tctttacttg
     1141 agttaaactc tccgttcttc tggttactat caattacatt atttgaatta gattgttttt
     1201 cctctttgtt tttttctttt tcgtttttat cacttaaatt atttgttaca attttgtaaa
     1261 gcccattctc cgttacaata ttgaaattac catcgctatc acgtataaca gattctttcc
     1321 catttgcatt agatttgatg aatgatatat acttaccgga taaattataa aattggttat
     1381 ttaaaacggt tattttaccc tttgaatcct caataacaat tccttcttta ccaccgaata
     1441 tactattctc tgagactact aaattatcac taactcgtaa taattcaata tttggttgtt
     1501 ctgtttcctt agaattatta ttaattacat tttttgttac agtaatatct gatacttttc
     1561 ctaaatattc tgcactatct tttgcaactc gtatcgcttt tgttttagga tcggcaatat
     1621 taaatatatt ttcggcgata accacctgtt tatttaaatc taataggtct ttagtgtttt
     1681 tatagctata agcatttact aaagctgctc cgctttctcg ataatgtaca ctctctcctt
     1741 taactttctt atcaaagcga tttcctttga ttaatacatc agtgaatcct gtaaaacgta
     1801 cacctgcata catcatgtta tcaaaatgat tatttaaaat tttaatatta gaggggttct
     1861 gtgtcgacaa tgtttgatag tgtgtgccaa ttgctgttac taattcccca gatttatcac
     1921 ttttgccaaa ataggaattt tgaatagtca cattttcaga ttttttccca tcatcattca
     1981 aggcataagg aaaacctttt ctagttaatg gttcaatctg aatgctctcc ttacttatga
     2041 tttgcccatc cttcatcgtt ttgggtaagg cttgcccaag aaaacgagaa ttatcaacta
     2101 atacattttt cgaacctgca atttgaatag catgcccttg ataactatcc ttgaatgtta
     2161 cattttttat agttacgtta tttgaattcc caatagcaaa tgcacctgaa gaatttataa
     2221 gtggtagatt ttttgcttta gttccttctt cattcaaagc accgttcata tcaatcgtac
     2281 caccagaata actaatatct tctgttgggc cccattctac ttgcgcacca tcatccgtaa
     2341 ataaacctgt cataaaaaca atggaagggt gattcttaat atttatacca tttagaattg
     2401 tagctttctc attcaattct aagtgtgtat gactttttaa aaaaacaatt tcttttacta
     2461 aataagttcc ttcaggaaaa tatacatttc ctccacctag cccttgagct gcagcatcta
     2521 ttgcatcttg aattgcttgt cgatcatcat gaatcccatc acctaccgca ccataatctt
     2581 ttacacttaa agcagtatct tcaaattctt ttttgataat tctgtcactt ataacatctt
     2641 ccttataact cttattttca ttagtagata ttactgtaga ttcaactgtt ctgttgtcta
     2701 attgtgaatt ggaacgaata gtaccattag agctgatact attaacactt aacgaattat
     2761 ctaaaccatt agtggttgaa ttagaggcaa tcatttcagc agaacgattt tgattaacag
     2821 ttggtgaatt atctaaatca ttagtgattg cattagaaga ctcttttgta gccatcggtg
     2881 aagaactagt aacaacttca tccgataaaa taccttggct tcccaaaatt gaaattgcca
     2941 atagtaccga gcaagtcccc acctttaatt tacgaatact gaatacctca ttaggaacaa
     3001 aatatttcat cagaacctcc aattttttta ctatttttaa caattgatta ttacttttct
     3061 taaaatagat agaatgacac cgctgtcatt atctttatca tatattttcc aaattgaatt
     3121 gtcaaggaat aacacaagaa aatattaaaa tttatactct tcggaaatct cttcaaacta
     3181 ggtcagtgcc gccttgccct aagtctaggt tactgacttg gtcagttcta tagtagactg
     3241 aaactataat agtacacctc tacttctaaa acattgttag aaatcgattt gactgtcccg
     3301 atcgatttgt cctattctta tttcatttta ttatatctac aacctcaaag cagggttttg
     3361 agctatctat ggccaacttc ctagtttgct ctttagcctt ccattcaaaa gtgatgctat
     3421 ctttcttagc ttgaatataa aatatcccat ttactatatt taaatttgtt gatatgttgt
     3481 aaattataaa tgaaagaaca aatatcttgt catacaagca ttcgccaggg atatttacat
     3541 gaatatcgct catatggcgg attgaaacta aaacactaca atatatttct taaatagacc
     3601 ttctataaaa gctagattta acctcgataa aaatagatgc agtgcttcta aaatactgga
     3661 atagggcata taggtaatcg tagacaagtg ttttatagat tgtctcgcgg gagatctaat
     3721 gatgtatagc aagcatcact aaacatatag aatatttgtg ttattataat gtcaatatgc
     3781 tataaattct atcatgcaaa agaatgacta gaacatgaaa gacaggcaca aaactttgaa
     3841 caatattctg aaaacaaaaa aagacgcgag tttccacaat ctcactcatg gaaactcacg
     3901 tctttcaaaa aatagggtga attaaaaact agaacaagac actagatttt ctaaatgatg
     3961 tctagtaagg attctaaatg ctacaccaaa ttgctcacat cttatttcaa accacgggac
     4021 tcccacaaga aaggaaaatc ctataaaaat tctctctctg aggagagaga ggcctcaact
     4081 acaggtgtaa actattatca attgaaaaca acagttttta cactcaatta gactttttag
     4141 ggacaggatc actttcgctt gactgtagtt tgtcattttt ttgataaagt tagcactttt
     4201 tcagaaactg ttttgaaagt ctcaatgata tagtctactt cttcatcgct taatttagta
     4261 tgaagaggga gggtaatttc attctcaaag aaggcatagg ccttaggata gttcgtcata
     4321 tcaaatccaa gattcttata ggctgtcaag agaggaagcg gtttgtagtg aacattactt
     4381 gcaattcctg ctttagccaa ttcttggatg atgaggttgc gttcttctag gcttgctcct
     4441 tctacacggg tgatgtagag gtggcgtgaa gattcgacag tttcagtctt gtgtgccaaa
     4501 ggatggatgc gagaacctgc aaaaccacta tcatagcggt ccacaatgtc cttacggcgt
     4561 tgcaacaaac ttggatagcg gtccaattgt accaaaccaa gtgaagccat gatatcggtc
     4621 atgttgcact tataggctgg tgtaacgata tcgtattccc atgaccccag ttgcatcttg
     4681 gcaagagcat ccttagtttg cccgtgaagg gaaaggattt ggaattcctt gtacatctct
     4741 tcgtcatcaa tcactggatt ggctttccaa gtcgcacttc caccttctgc cgttgtaaag
     4801 ttcttaacgg catggaatga gaaggaagta aagtcagcga tagaaccaga aggttgtcct
     4861 ttataagtag atcccaaagc gtgggcacta tcagagacaa tgacaatacg gttaaaggcc
     4921 ttttgccact tgcttgaagc ggtaaagaag tcacgttttt tctccacgac ttggaacaaa
     4981 cggtcataat cgcaaacaat ccctgcgagc tctactggga taatcacctt agttttctca
     5041 gtgatagctt gctcaagcag gtcatagtcc atctcaaacg tatctgcttg gatatccacc
     5101 atgacagggg ttgctcccac gtgcgtaatg acactacatg aagccgtata ggttatggct
     5161 ggaacgatga cttcatcacc aggtcccact tcaaaaacgc gtaaaatcaa ctccagagcg
     5221 gctgtcgcag agttgagaca aacagtctta ggtgtctgtg tgtaaagaga caagcggcgc
     5281 tccagttctt ttgttttagg acctgttgtg atccaaccag aacgcagggt atccgctact
     5341 tcagcaattt ctgcttctgt gatatcaggc ggtgaaaatg gaatattgta atttggcatt
     5401 gatttgctcc ttttatctgt actcattctc ttatgactac tttattttag taccttaaac
     5461 acggtttgaa acatgatttt gatgtcccca aagaaactaa actctcggag ataggcgagg
     5521 ttatagcgca tcttttcagg aagaacatgc tccacatagg cctgatcaac tgacagacct
     5581 ttctctgtca tttgactgat gatggtgtcc tcatccttgt agttgatgct ggctggagag
     5641 gtaattcctg cttgcaagag caaggttgcc atcatttcag tgctatactg ctctgtataa
     5701 cgtggcactt caggtcgtgt accgacaaag gacatctcac ctttaaggac attgaccaac
     5761 tgaggcagtt cgtccaaacg gacacgtcgg atgaaatttc caaccttggt aatacggcta
     5821 tcgttagcag aagtcaccag atttcctttt ttatccgcat ccgtcaccat ggtacggaac
     5881 ttccaaatct tgaaccgacg gttgtactgg gtcacacgct cttgcttgta aatcactggc
     5941 cccttgctat ccaacttgat ccaaatgctc aagatgagaa agatgggaga ggtcagaacc
     6001 agtaagacca aggccaaaac ccagtccaag caacgcttga aaatcagcga acccttcctt
     6061 ttagagacaa gctggtagta agactcaacc tcgcttgatt tcatttccac aggcaagtct
     6121 tcccatttca gcatgctgtt ctcctttgtt tttattatac tatttgtcaa cactttttgt
     6181 caacactttt cattatacca caaaatggaa aaggcggtga aaaaaatctg taatttgaag
     6241 gatttccttc tagtactcaa tgaaaatcaa agtacagctt tgagattgca gataaagctg
     6301 acatggttta aagagatttt caaagagtat aggccatggc ccctgcctgc aaactataca
     6361 tcttgtgata ggttcctccc agagccaaga gttcctcatg ggttccactc tcgataatgc
     6421 gtcccttatc caagacatag atgcagttgg cgtcttgaat agtagaaagg cggtgagcta
     6481 tagcaatagt tgttcggccc tgtctcatct tcgccagaga agcttgaacc aagctttctg
     6541 tttcagagtc aatattggct gtcgcttcat ccaaaatcag gattttaggc tggctggcga
     6601 ctgttctagc aaaggcaaga agctggcgtt gcccagtaga gaagctcgaa ccacgctcgg
     6661 aaacagggga gtcgtacccc tgaggaagtt cttgaataaa ggaatctgca tccacaaagg
     6721 ctgccgcagc ctgaacctgc tcatcactga tttcttggta catggcgata ttggacttaa
     6781 tagttccatg atagaggaag ggttcctgca agaccaaacc gatgtttttt ctcagctctt
     6841 cttgactgaa atccctgata tccacatcat ccaagagaac tctccctgac tggaattcat
     6901 aaaagcgcat gaggacattg ataatagacg atttccctga acctgtatga cctacaaagg
     6961 caatggtttc acccttatta acagagaaag aaatgtcatc cagaatcgga tgtttaccgt
     7021 catatgagaa acacacatgt tcaaagcgga tattgccttc ttggactttg gcttgcccat
     7081 tttcttgaag aggttcatag gtcctctcgt ctaccagggc aaagacacga cctgcagaaa
     7141 ccatagacgt ttgcagtgtt gaaaagtttt gcgtcacctc aatcaaaggg tcaaagaggc
     7201 ggttgatgta ctggataaaa gcatacatgg tcccggccgt tatcccgata gaaagaccac
     7261 gatagccaaa ataggccatc aagacagcat aacctagaag tttcaacaaa ctcatggcag
     7321 gtctcaaaaa gagggcatcc aaggctacag aacggttggc atagaccaag tgctcttggt
     7381 tgatttcatc aaattctgcc tgcaggcgct tctcttgatt aaaggcctgg attattctga
     7441 ttccctcgat gttttcagcc agcttgctat tgatatctga caagagactt ctggttttct
     7501 caatgatttt cactgatttt ttccgataga gattgaccaa aaggaaaatc aaggggagaa
     7561 agagcaagac taatgctgtc aaacgaaaat ccaataccaa catggtataa agggttgtca
     7621 gaaagatgaa aactgctgag ataaagctag ataaaatccc agaaaacata tcactaatcg
     7681 tctcggtatc gttggtcaaa cgagaaacga tagaacctgc tggcgtcttg tcaaagtaag
     7741 acatgcccag tttctccata ttggcaaagg catcccgacg aatatcccta acaatactgt
     7801 aagacacgcg cgcaaagaga agattgccga catactgaac tacagtttgt aggatgtaga
     7861 gaccatagta gaccagcaaa acggtaacgg ctagttggtt aagattgctg agatactggt
     7921 cgataaagtg ggaagccacg aggggtatga cacttttaat gaccgtcgtc gctaggagaa
     7981 aactgagtgc caaaaaggtc aggagtctat aaggcttgag ataagacatc aagcgcttca
     8041 atacagccca ttgttcttgt ttattctgca tcttcttctc ctttcatttc taactgctga
     8101 gactggtagg tttgggcata ccagccatcc atcgctagca agtcttcgtg cctgcctcgt
     8161 tcgataattt gaccattttg tagaactaaa ataaaatctg catggacaac agcactgagg
     8221 cgatgggcag ttataatggt tgtcttgtcc tttcgcgtct ccttgagatt gtcgataatc
     8281 gcatactctg tcttggcatc tacggcggat aaggaatcat ccaaaatcaa gatatcaggg
     8341 tttaaaatca tagcccgact catagccaac cgttgctttt gaccaccaga aagactgact
     8401 cctttttcac caatcagcgt atcaaatcct tgaggcatgt ctacaatatc ttggtaaact
     8461 cgggctagct tagtagcttc ctcaacctct gaaaggggca agttaggatt gccaaagcgg
     8521 atattgtcta ggattgaagt cgcaaagaga aactggtcct gaggaacata gcccatgaga
     8581 ctgcgaagtt ctgtcaaacg atagtcccga atatcgtgac cgtttagata aatggcaccc
     8641 ttatccacat cgtattcacg caagaggagc ttgattaagg acgttttccc agagcctgtc
     8701 tgcccaacca agcccagtgt ttgccctttt gccaaactaa agtgaatatc cgtcagtgtt
     8761 tcctcatttt caaaagcaaa gctgtcaatg gcatactcca aacgcccatt ttcaatacca
     8821 tccagaggaa actcagggtc ttgtacagga gattcctgag acaaaagatt ttcaatccgc
     8881 tggtaagaaa ccttccctcg ctgagtagta ttaaagagga agccgatggc cataagaggc
     8941 cagaccagca tatccaaata gctgataaag gtgactagat taccaactgt aatctgccct
     9001 tcctgaacca tcaaggagcc aaccaaaagc gttaaaacat aggacgaacc aacaaacaag
     9061 agaaccatag ggtcaaagag actatcatat ttcatggttt gcaggttctt ttggaaggtt
     9121 aattcattga ctgcctgaaa agacttcaac tcgtctgcct gataaccgaa agacttggtc
     9181 actttgatac ctgatacgga ctcctgtacc ttgttattga gttcagaaaa agcagcttgg
     9241 gattcgccaa aggccttatg agtctttctc cctaggcgac tagtcgcata ggccatgaaa
     9301 ggtaggggga gaatggcaac aagagtcatc tgccatgaga tgctaaagag catggtcaac
     9361 aaagtcacca gagccgtgat agaggcatcc accgcagaca tgacaccgcc acctgctaaa
     9421 cgagtcaagg cattgatatc attggttgcg tgtgccatca gatcacccgt ccgataggtt
     9481 tgataaaagg ctggcgacat ttttgtgaaa tgcttaaaca agcgagaccg catgatctgt
     9541 cccaagcaat aagaggtccc aaggatatac atacgccaca catagcgcaa atagtacata
     9601 ccaaaggctg caagtagcaa gtaaaatagg ctaagaagga ggtcctgctg ggttaattgc
     9661 cccgatgtga tggcatcaat gactcgcccc ataaccatag gaggaatgag attgagaacg
     9721 gaaaccaaga ccagggccac aatcccgact agataacggt gtttttctaa cttgaaaaac
     9781 caccagagtt tttgaataat ggacataaaa tccctttctg attgcaaatg gaaacctgag
     9841 gccaatactc aatgaaaatc aaagagcaaa ctaggaaact agccgcaggc tgctcaaagc
     9901 actgctttga ggttgtagat agaactgacg aagtcagtaa cctacatacg gcaaggcgac
     9961 gttgacgcgg tttgaagaga ttttcgaaga gtacaagacc ccaggttttt cttattcata
    10021 agttacgact gtaacagcac ccttgtcata ttcagcaata aagatattgg ctacattgtc
    10081 atgcccttgt ttgctgaggt tatcaagcaa ccactcttcg ctacgaccaa tcgattccaa
    10141 gacgtctact tgaatcacac cgtcagtcac aactggatac ttaggatttt catctcccat
    10201 ttgcacaacg atgagttgcc cattttgctc ttgcacagct cgtttgactt gcttcatctg
    10261 gaaaatccct tggctacgaa gtttgagggc tacatccgat gcagacaaac caactgaacg
    10321 acaggcttat gggtcaatat tcccattttt gataaggaga gttggcttac catcaatcaa
    10381 acgtttcaca aagtgaacat tgttgttgag ctatttgaga gtcaagatca gtttgattaa
    10441 aatttcgata taattaagtg tcatttgttc acatccacca gttcaattgc gtctgtctta
    10501 tacaattcga ctttctctaa caggtacttg tctggctcac ttccgtttag ggcacgataa
    10561 tagcgatctc ccaccttgat aagtgcgcca tctgtgtttg tggaagtatt aacatggact
    10621 tctgacttgt ctactcccaa atctttggaa acaacctcga tgaaatgaag tgaagtttga
    10681 aatttattgt tagaaacttg attgttttga taagtcgtga tgctgaccaa aagcatagct
    10741 aaataaggcc agagctgaaa tcatgaccaa ctcacgaaat ttggttcctt ttttatcatg
    10801 gtatgccttg aaagcaaaaa aaactgtcac agctaataga acaatcccaa agccaaccat
    10861 gataccattt tgctgaccga tttggctgag tacatagtca taagagtaga atttcatgta
    10921 ttttcactcc ctttcataaa atcattctta attataaacg aaagagagta atttttcaaa
    10981 ccatacgttg gggaaataga tctttttttg gtataataaa atctataatc tgaatgaaaa
    11041 aggtaacttt atgaaactta tctcatggaa tattgattcc ctaaacgctg ccctaactag
    11101 tgactcagct cgtgccaaat tgtcccaaga agtcctacaa accttggtcg ctgaaaatgc
    11161 tgatatcatt gctatccaag aaaccaagct ttctgccaaa ggacctacaa agaaacacgt
    11221 ggaaatttta gaagaactct tcccaggcta cgaaaacacg tggcgctctt cccaagagcc
    11281 tgcccgtaaa ggctatgctg gaaccatgtt cctttataag aaagaactta cacccactat
    11341 cagcttccca gaaatcggtg ccccttctac catggacttg gaaggccgta tcatcactct
    11401 agaatttgat gcatttttcg taacccaagt ttacactcca aacgctggtg acggtctcaa
    11461 acgcttggaa gaacgccaag tctgggatgt caaatatgcg gagtatttgg ctgaactaga
    11521 caaagaaaaa ccagtccttg cgaccggtga ctacaacgta gcccacaatg aaatcgacct
    11581 tgcaaatcct gctagcaacc gccgttcacc tggatttact gacgaggaac gtgctggatt
    11641 taccaaccta ttggcaactg gatttactga taccttccgc catgttcatg gcgatgttcc
    11701 tgaacgctac acttggtggg cacaacgcag caaaacttct aaaatcaaca atacaggctg
    11761 gagaatcgac tactggctca caagtaaccg tatcgctgac aaggtcacta aatctgatat
    11821 gattgactca ggtgctcgcc aagaccatac accgattgtc ttggaaattg acttgtaagg
    11881 attttctata tggactacaa tgcggtcatt cccgagtttc ttgtatcgaa tatcgaacag
    11941 tcccggtcct tctactgtgg tttactgggt tttaggattg aataccaacg ttcagaagaa
    12001 aactttctct ttctcttgaa gagtgtcaac taatgttaga ggagggaaca aaggatcaac
    12061 tagctgaact gacctacccc tttggtcgtg gagtcaatct ctcctttggc ataaaggatg
    12121 ttccaaaact ctatcaaaaa gtgatggagg caaactatcc tatttatcgt cccttgacca
    12181 aaagaaagtt tcgtgtcagt gatccctata tctatcctca taaatttgca gttttggatc
    12241 cagatggcta ttttttaaga tttagcgagt agaataataa aaattgggaa aagtctttaa
    12301 aagttagaaa aaggcatatt gtcaggtgtc taaaaacttg atagtatgcc ttttatgatg
    12361 gaaaaatctt ctaatactct tcgaaaatct cttcaagcca cgtcaacgtc gccttaccgt
    12421 aggtatataa tactgactct gtcaattcta tccacaacct caaaacagtg ttttgaggtt
    12481 gtggatagct tcctagtttg ctctttgatt ttcatttagt ataaatgtta gcttacaatg
    12541 gagtttttgt tcaccctctc ttctgtttgt tcgtttttta aaccaaaaca ctaacaaaat
    12601 agttgatttc taaagttatt tagtgtaaaa taaaaacatt ggctcaagcc tatttaaaat
    12661 tgaatatcgt tttacttgtt tatgtcgtga attggcacga cgtttctaca aggtgccgga
    12721 acacctaaca ataagtaagt cagcagtgag atatggccgt ttttgccatg cctatttggg
    12781 ggacttactt agagattaag taggtctttt ttgaatattt ttaatatttc ccttaattag
    12841 tgcggacggg aggtgaaatt atcctgtgaa taatttcagg aatccaggga atttcatccc
    12901 taatttttga gcccctagct ctttcatttc taggcttagg ctaaaatagt tccccgaact
    12961 attttactct caaaaatccc gtcatagaca ataacaagat tattgtctat gataccacta
    13021 caacgggaaa tatttgataa gactcgcttc gctcgtaata gttttatcaa atatatagca
    13081 ttagcaaaag ggatcactat agaaacattt aaacaacaag taaattaact tttagacttt
    13141 aggaggtctt atgaaattat tagaagagcg catcctcaag gatgggcata tcttgggtga
    13201 taacatcctc aaggtagatt cctttttaac ccaccaagtt gactttagct tgatgcgaga
    13261 gattggtaag gtttttgcgg aaaaatttgc tgctgccggc attaccaagg tcgtaaccat
    13321 tgaagcgtcg ggtattgccc cagccgtttt tacagctgaa gccttaaacg ttcccatgat
    13381 tttcgccaaa aaagctaaga acatcaccat gaacgaaggc atcttaactg ctcaagtcta
    13441 ctcctttacc aagcaggtga ccagcaccgt ttctatcgct ggaaaattcc tctcaccaga
    13501 ggacaaggtt ttgattatcg acgatttcct tgctaatggc caagctgcta aaggcttgat
    13561 tcaaatcatc gaacaggccg gtgccacagt ccaagctatc ggtatcgtga ttgagaaatc
    13621 cttccaagat ggccgtgatt tgcttgaaaa agcaggctac cctgtcctat cacttgctcg
    13681 tttggatcgt tttgaaaatg gtcaggtcgt atttaaggag gcagatctct aatgcaaact
    13741 caagaaaaac actcgcaagc agccgttctt ggcttgcagc acttactagc catgtactca
    13801 ggatctatcc tggttcccat catgattgcg acagcccttg gctattcagc tgagcagttg
    13861 acctacctga tttctacaga tatcttcatg tgtggggtgg caaccttcct ccaactccaa
    13921 ctcaacaaat actttgggat tggactccca gtcgttcttg gagttgcatt ccagtcggtc
    13981 gctcccttga ttatgattgg gcaaagccat ggtagtggcg ctatgtttgg tgcccttatc
    14041 gcatctggga tttacgtggt tcttgtttca ggcatcttct caaaagtggc caatctcttc
    14101 ccctctatcg taacaggatc tgttattacc acgattggtt taaccttgat ccctgtcgct
    14161 attggaaata tgggaaataa cgttccagag ccaactggtc aaagtctctt gctttcagct
    14221 attactgttc tgattatcct cttgatcaac atctttacca aaggatttat caagtctatc
    14281 tctattttga ttggtctggt tgttggaact gccattgctg ctactatggg cttggtggac
    14341 ttctctcctg ttgcggtagc tccacttgtc catgtcccaa ctccactcta ctttgggatg
    14401 ccaacctttg aaatctcatc tattgtcatg atgtgtatca tcgcaacggt gtctatggtt
    14461 gagtcaactg gtgtttatct agccttgtct gatatcacaa aagatccaat cgacagcacg
    14521 cgccttcgca acggttaccg cgcagaaggt ttggccgtac ttctcggagg aatctttaac
    14581 accttccctt acaccggatt ttcacaaaac gttggtttgg ttaaattgtc aggcatcaaa
    14641 aaacgcctgc caatctacta cgcagctggt ttcctggttc tccttggact gcttcctaag
    14701 tttggtgccc ttgcccaaat cattccaagc tccgtcctcg gcggtgccat gctggtgatg
    14761 tttggttttg tatctattca agggatgcaa atcctagccc gagttgactt tgctaacaat
    14821 gaacacaact tccttatcgc agctgtttca atcgctgcag gtgtcggtct caacaacagt
    14881 aatctctttg tcagcatgcc gacagccttc caaatgttct tctcaaacgg aatcgtcgta
    14941 gccagcctac tcgccattgt cctcaatgcc gtattaaatc ataaaaagaa ataagaaaaa
    15001 gaggtgcgag cctctttttt gtttatcaca aattgattat aaatacaatc aatcccaatt
    15061 cccatctaat tgaattggac ctatttcaaa ttctttaaaa tattatgttc caaatcgtta
    15121 atattatata agaatggtaa aatatcaaat gtttctcgta aattattctt agccttatac
    15181 caaccttgtc catcagtaat ccacataaac tcaacattag gaatagcttt aatttcttca
    15241 gcaatcattt tgtaagatct agccgtctca tttaattttg aaccacttcc tgaataaaaa
    15301 ttaacctcaa tcagataaag cacttgttca ttttttatga caaaatcaaa acgtttcaca
    15361 gtattcccat cattcgtaat agcagataaa tcgacagaaa aaatttcctc tatttcattt
    15421 tgttcaatct ctttaaataa attctctcct aaaatatacc cttcagcttc aagataactt
    15481 tgaacaatat tttccatcgc atctccagtc cgatttttgc gcccattagt atccatacca
    15541 acctcaacac ctgtaacgta gtcaactaaa tttgatacta aatgattttg caataaatca
    15601 aatatacctg atttttccaa aaacatagta tattcttcaa tactatagtt tcgtttacta
    15661 aaatcaaaat agaaatcttt tatcggatcc ttaacaataa tcgtatctct caaacgttta
    15721 gcaataagta gaggcacaac tttcaaaatt tctggataat tttgatataa gtctaaaaaa
    15781 tcctcttgta tatttttact accaataaga ctattcataa tatttagagc aacttttata
    15841 ctagatacat ttttataaac cttgggaaaa tctgtataat aagtccagtc agctactgtg
    15901 tcggtcatcg ttgacaacca ctcatcaaaa tttctagttt gtttcatttt ctgcctccaa
    15961 cctcttcctt gcaattttta aatattcttt ttcagcatcg atacctataa atcttctacc
    16021 taaccgcttc gcaacaacac ccgtagtgcc actaccaaca aatgggtcta gaatatagtc
    16081 accctcttta gtagaggcta aaataatgcg ttctaacaaa tactctggtt tttgagttgg
    16141 atgtttccca gcccattttt caactttctt tgttaaagaa cccgtccaga catctttcat
    16201 ttgttttcca tcattcaatt ctttcattaa atcataattg tagtaatgac gagctttttt
    16261 atcatttttt ctggcccata aaatggtttc agtagaatgg gtaaaataac gacaagataa
    16321 attgggggca gggtttgttt tctgccaagt aatattattc agaattttaa aaccttcttg
    16381 ttctaatgcc attccaactg agtatatgtt gtgcaaacta cctgaaatcc ataccgtccc
    16441 attaggcttc agaacttctt ttgctaggcg aatccattta cgattaaact catgtttttc
    16501 ttcgaatgaa gaaattttat cccaatctcc tttatcaaca gaaacaactt gtcccccaga
    16561 attagatatt ccaccattac ttaaaaaata aggtggatca gcaaaaatca tatccatact
    16621 ttctggtttc atttttgata agaacttgaa cgtatctgaa tgaacaagaa tcatcttatt
    16681 tttattgtag tatggttttg tcataagaac acctccatac ttatattcgt tatttttcat
    16741 aatttgtgac tataatttca gaaatttttc ctcgacttga agattttgct ccattagttc
    16801 gggtagcttc aacataatgt atattaaaat ccttatacaa ctcctctact aaagcactag
    16861 aagaatttga taacataaca taagctcctg tatcactcaa tctcttaaag gcatctctta
    16921 atcttacttg atctgcaaaa gagaatccct catgagtata agacgtaaat gcactcgtct
    16981 cagacaatgg aatatatgga gggtcaaaat acacaaaatc tcctgttcga acatctacaa
    17041 ttgccttttc aaaatctccc actttaattt ctagttgatt gttatttaga taaactgaaa
    17101 tagcagatat caattcctca tcaacaattt taggattctt ataacgtcca tatggaacat
    17161 taaattggtt cttagaattc acacgatata gaccattaaa gttcactctc aacatatata
    17221 gaatacgtgc agctctttgt acttcggaca tcatatctat tctttcatca cgatctgcag
    17281 aacgtaaatc taaataatat tcttttgaat tatattcctg atgaactttc aaaatttcaa
    17341 tcaattcttg aggattgtcc ttaatttgtt gatagcaatt tattagttca gcgttaaaat
    17401 cattaataac tgcatcttta ggagccaaat caaaaaataa agctccacct ccaacaaaag
    17461 gttcgaaata cctgttatag gttttaggca ttaattctct aataacaggc aataattgtc
    17521 ttttaccacc tgtccatttc gtgaacggtt gtaaagtaac tttctttatt tcttttatct
    17581 tcatgctttt aattatacca tagaaattaa aacacaactc ttgtattttc taccacatta
    17641 ctaaattttc tattttagaa ttaaatcata tcaatataaa aaaatgagta aggcaaatct
    17701 tataaattcc aaaattcacc tcactctatt aaattactct ttatatcctt acccgtcttt
    17761 cctcagacct ttggcctgtg acaaacatag taaaggttgc tttgcagaca tttctgccct
    17821 cttgattggt gatatccaca tccatcacac aggttgtacg accttgatgg acacattctc
    17881 ctttaatggt caatacatcg tcgagttttc ctgccttaag gtagttgata gaggattgga
    17941 gtgtcacccc atcaagtccc agcgagataa ccaccaaacc actgatttgg tcacatagtg
    18001 tgaatagata gccaccatgg gcattgccat agtagttgag cgacgagttc actactttgg
    18061 tcgtcaccac aacgtgacca tctctcattt tttcaatttc gtaattttca aaggcagata
    18121 tagcgtcaaa atgaaagtct ttcatcgttt cctcctgata tttcaaatga cactatgata
    18181 ctacttttta taggactgtg caaggagaaa gctcctagtc tagtaaaatt ccgacatgtt
    18241 ccacaaagcg cataaaggct gtctgccctt gttctgtctg cttgtagact cctgcatcct
    18301 caagtacacg cgcaaagata gcacccacag agtccttgac gattgcaagg gctttttctt
    18361 tatccgttag gtctggatgt tgggatttga gttggtctgc ccactcctga tgataatcgg
    18421 caactgtaac agcttctcct acaagatagc tagcgacttg ctctacttct tctttcagac
    18481 gtggtggcaa gattgccaag cccatgacct caatcaagcc gatattttcc ttcttgatat
    18541 gttggacatc cttgtgggga tgatagatgc catcaggatg atctgctgaa gtctgattgt
    18601 ctcgcaagac caagtccaac tcaaactgtc catcacgctt acgcgctatt ggtgtgatgg
    18661 tgtgatgcgg tgtcccgtct gtctctgcta aaatctgcac actagaatct gaatactgac
    18721 gccattcctg gaaaatctta tcagctaagt taatcaaatc ctctttggaa tccgaagtca
    18781 agcgtaggac agacattggc cacttgataa ttccagcctt gacctgctca aaaccagcaa
    18841 atcggaaggt cttttgcaag ggagccaatt ccataggaaa tacgtgacgg cctccctgat
    18901 aatgatcatg agttagaata gagcccccca caatcggcag gtcggcatta gatccagcaa
    18961 aatatcctgg aaactgctct acgatagcca acagacgttc aaaactctga cgactaatgg
    19021 ccatgggacg atgctggcca tctaaaaaga tacaatgctc attaaagtaa gcatagggcg
    19081 aatactggaa accccattcc tgaccaacca tttcaaaacg gataatccgg tggttgctgc
    19141 gagctgggtg gttgacccga ccatggtaac cctcattctc taaacaaagt tgacactgag
    19201 gataattact agcttgcacc aacttggcta ccgcaatctc tttcggatcc ttttcaggct
    19261 tagagagatt gatggtaatt tcaagttctc cataatcaga tggaacacga taaacgatat
    19321 ttttagcaat ggccttgagt ttgatgtagt catttttctg actgagttga taaaaatcct
    19381 ctatcgcttg ctcaggagag taggcatagg cttcccagaa gtcacgattg acctgactcg
    19441 gacaaggagt caccaagtcc atcaattcag taccaaggat ttcacgcgca gtctgactat
    19501 cctcaatcgt ctctaatcga acggcttcct caactagctg gtccttgagg tcaatcaatt
    19561 tatccagatt ggtctcaact tccaaaacac cttctcccac tcgtgccaaa acacgattgg
    19621 tcaggtagat tcgatccatt tcctcaaatg aactttcaga aatgacatgt gttacaaatt
    19681 tatctactaa ggtcactata gagcctcctt ttgactagtc aaggacgcga gtgccacctg
    19741 caacttcagc gatatagaag cttggagcgt atccaactac ttcctcgtag tgtttgccta
    19801 cagcttcctt aaaggcctca acagtatctt tttgaaccaa ggcaatggca cagccaccaa
    19861 aaccagcccc tgtcatacga gcaccgagaa ctccttcttg tgcccaagct gtgtgaacaa
    19921 gagtatccaa ttccaaacca gttacttcat aatcatgctc cagagaaacg tgtgacgcat
    19981 tcatcaaacg accaaatgtt tccaaatcac ctgcttgaag ggctgcttga gctttaaggg
    20041 tacgttggtt ttcaaacaca gcatggcgag cacgtttcaa acgattttca tctttaatca
    20101 gatagctata ttggtcaacg gcccactcgt ccaattcacc cagggtctga atatccaagg
    20161 caacttgcaa ttcttccact gctttttcac actcagcacg acgttcattg tatttagagt
    20221 ccgccaattc acggcgtttg ttggtgttca tgataacaac gacattgtcc ttcaaatcaa
    20281 gtggcaccaa gtcgtattct aaagtattgg tatctaagta aatagcacgt tggtcagccc
    20341 ccataccaat agcaaattgg tccatgatac cagagttgac tccgataaag ttattttctg
    20401 tttgttttcc gattttaacc aaatcgagac gctctagttt taaatcaaag agatgctctg
    20461 ccacgactcc tgtcaagagc tccaaggatg ctgaagaaga caaaccagca ccatttggga
    20521 tattcccata aacataaaaa tcaaaacctt tgtcaatcac gtgcccagct tcttgcaaga
    20581 aatgaaggac accttttgga taattggtcc agttgtgctc tttttcaaac ttgaggtcag
    20641 cgagaggcac ttcgataatg cccttgtcct caaagttagc tgagtagaaa cgcaagactt
    20701 ggtcgtcacg cttacgagct gcaccgtaag ttcccaagga aatagcagca ggaaaaacgt
    20761 gcccaccgtt gtagtctgtg tgttcaccaa tcaaattaat gcggcctggt gaaaagaagg
    20821 tttggtctgc ttcttgacca aaaacagcaa gaaagtcttt gcgaagggct tcagtagtaa
    20881 gatgttgtgc catatgaatt ctcctttgac tgtccgttaa gccaccttaa cgtataatcg
    20941 ttttctttct attgtatcca aggaattttc caagatcaat cctttttact aaaattttag
    21001 taaaatatca gtaaaaagaa gaaaaacatg atatactaac ctaatcaagg aggatttatg
    21061 gctaccttaa aagacattgc acagctagcc tctgtctcta tcgcgaccgt atcccgcgtc
    21121 ctcaatcgcg accagagcct atctgttaca gaagaaacca gacaccgtat tttaaccgtt
    21181 gctgaagagc tgggctacac caagcacctc aagacaggcg agtcccacaa acccaagcaa
    21241 aagattgcca ttatccaatg ggtcagcgaa caaggggagc tggacgacct ctactactac
    21301 cagattcgcc taggaataga aaaaagagcc caagagttgg actatgatat cttgcgctat
    21361 tttaatgacc atccttttac cttaagcgag gaagtgattg ggattctctg cattggaaaa
    21421 tttagccgag ctcagatttc tgcctttgaa gaataccaaa aacccctggt ctttctagac
    21481 agtgatacac tttctctggg gcatacttgt attattacgg atttttacac tgccatgaaa
    21541 caggttgtcg attatttcct cagccaagga atggaccgta tcgggattct aacaggcctt
    21601 gaagaaacaa ctgaccaaga agaaatcatt caggataagc ggctggaaaa tttcagaaac
    21661 tacagtcaag caaaaggaat ctatcatgat gaactggtct ttcaaggaaa ctttactgcc
    21721 cagtctggct atgacttgat gaaggaagcc attcagaact tgggagacca actcccacca
    21781 gcatttttcg cagccagcga tagtttagct atcggtgccc ttcgtgccct ccaagaagct
    21841 ggaatcagcc taccagaccg tgtcagcctc atttccttta acgacaccag cctgaccaag
    21901 caggtttatc ctcccctctc cagcatcaca gtttatactg aagaaatggg gcgagcaggt
    21961 atggatattc ttaacaagga agtcctccac gatcgcaaaa ttcctagcct gaccatgctg
    22021 ggaaccagac tgacattaag agaaagtacc ctaaatcaag aataggataa cataaaaaac
    22081 gaatagagtt ctaaaactcc tattcgtttt ttattcgatt acaatcatag acttaatggt
    22141 cttacgttca tccatatctt tgtaggcttg gtcgatatct tccagtttat aacttgaagt
    22201 aaagacgcga cctggattga tatcaccatc aaggacggct tttagtaaaa attgcttatc
    22261 gtatgttgta gcagaagctg ccccacctgc tacagagata ttttgcataa atgtcgaacc
    22321 aagagcacga ttattatagt gtgggactcc tacaaagccc atacgccctc cattatgaag
    22381 aacacctagc gcctgttcta tagcagcctc cgtaccaaca cattcaagtg ctgcgtctgc
    22441 tcctccaccg aggatttcac gcaccttggt aattccttct tgaccacgtt ctgcaacaac
    22501 agctgtcgca cctgactcca tagccatctt ttgacggtct tcatgacggc tcataaggat
    22561 aatttgtgat gctccacgca tcttagccgc gatgacagca cattgaccaa cagccccatc
    22621 accgataaca acaaccttgt cccctttttg aacatttgca acacgcgccg catgatagcc
    22681 tgtcggcatg acatctgcaa gagtcaaaag ggactggagc atcccttctg tatagtcaga
    22741 aggttgacca gggattttaa ccagcgccca gtttgcatag tggaagcgaa tatattctgc
    22801 ctgaaaatca ccccccaaat tattgccaat atgattgtcg caagaaccgt caaatccagc
    22861 aagacaggca tcacactcac cacatccatg tgtaaaaggg acaatcacaa aatcacctgg
    22921 tttcaccgtc gtaatggctt ccacagcttc ttcaacaatc ccaatcgctt cgtgtccact
    22981 atttttgtgt ccagctttcg tttctggatt acggtacctc cataaatctg aaccacaaac
    23041 gcacgcacga accacacgaa taatcacatc atccgcttct attatttgcg gacgttcaat
    23101 gctagcaagt ccaacctgac ctgcctttgt atatactgct gatttcattt aaaattttcc
    23161 ttccttataa agtttaattt tgagatttaa acgatttaaa gctgtctgta gctgagacaa
    23221 gcgctcctct aatttttgct tttcctcttc taaaatacca agcctctcct ctctcgtttc
    23281 atctcccttt tggtagagcg acatatagtc aactaaacta tctacagaga cacccgccga
    23341 acgaaaacac ttaataaatt ccagcgcttc gatatcctga tcttgaaaat cacgaatccc
    23401 agtagcagta cgagtaatcg gtggcacaag accaacccgt tcataatacc gaatcgtatc
    23461 cgctgaaatt cccaacaaat cactggcaga tttaatattc acacgctaac ctcctctcat
    23521 ttcattcact gaattcacgc tattctaact cacttatctt attatataac tttgagttga
    23581 ctccaagtca agcattttgt atctaatcga aaaattttta tcattaacaa tcatatatct
    23641 ggagtttata caatgctctt ctcttaccaa tttttgaaat atttaaataa taaagaaatt
    23701 actctattcc aaataagata gtacccaagt atttttcact tttttctaat gttgatgctc
    23761 ataactccgt tccaagtcac acacctttcg cttatgggtt tggtgagttt ctaggtcagc
    23821 atcaatttca atggtaatat tttgaaaacc acaatctttt aggaaaattc gaatagactc
    23881 tttacaagtt tccatatgtt ccatttcttt tagacaaaca tggacaatgg catttttttc
    23941 caaagcatcc atagtccaga gattaagctg attaaggctg gccacattgt ccaatcgctc
    24001 caggccactc tttacttgct tgatatcaag accttctggc acagcatcca agaaaatctt
    24061 gagtgtagac caaaaacgtg gaagggcttt tgaaagaata aagaaagaaa tgacaaggga
    24121 caaaagagga tctaggatat accagtccgt aaatcgaaga acaatcgcca tcaggataac
    24181 agctacccac cctagcgtat cttccagaaa atgcagactc agaatagact cattctttgt
    24241 ctttccctta ccaaccacca gactcgctaa cagattgata gtaatcgcaa taattcctaa
    24301 ccagagaatc ccctcatcat tgactggttg cggatgcaaa atcttcgtga cattttccaa
    24361 aatgactaga acagagcccg ttacgagaat cacagctgtt accaaggctc ctagcaggct
    24421 aaaccgctta tagcccaagg tgtactgatt gtcttcttca cgattggaga ttgtttctag
    24481 aaaagctgat attccaattg caatcgcatc tcccaagtca tgcacagagt cagcaagaac
    24541 agcgctagaa ccaaatactc cacctgcaat aaactcaaca atggcataag tcaaatttaa
    24601 gaaaaaagcc acccaaacag catattttgc cttcatattt ctcattcctt tgttataata
    24661 gatttatgaa caccttgttc attatcatta tccactaaaa ctcaaagaaa ggatagaagc
    24721 aaaacgtcag ctttattcag ttctgaacaa tttgccttaa gtgtccatat gactaacatt
    24781 gaccgccgta tcagcaaaac caaaaaagcc atctatcaag cttttataca acttttgaat
    24841 gctaagggct acgaggccac tactgttcag gatatcattg atctcgcaga tgtgggacga
    24901 tccacctttt actgtcacta tgagagtaag gagcttcttc tggaccagct ctgccgctac
    24961 ctcttccatc atctttttga aagagagcaa gccatttcaa ccgaggacta cctcgcccac
    25021 ctctttctcc attttcataa aaaccaagac catatcacca gtctgctatt ttccaaaaat
    25081 gactacttcc tccgtcaact ccataaagag ctagaacacc atgtctattc cgtgctagct
    25141 gataatttaa aagaagccca ccctaatctg cctacttctt acctccaaca cttggtcatg
    25201 tccaacttta tcgagacatt gacctggtgg ctcaaaaaag gtcaagattt cacagaccag
    25261 gaagttgtcc aattttatct agaccttctc attcctaaaa attgaatata gagtaaagct
    25321 tcagttgtct tatttctagg ttactgagtt ttttatcttt tcaacaacaa aagaggaccc
    25381 gccgatcctc tttttcatac tataaatcct tgattatcaa ctatatctgt tttaatcgaa
    25441 atctcaaaac agcactttca aacatctttt cctagttaag taaatcagta ttctgcttag
    25501 ctgccttgct ccattgatac caaccaacta gactgttaat gagataaatt agatatttcc
    25561 cttgaatttg caggctttct ccccaccaga gatagattga aaagacattg gtagccgccc
    25621 agaatatcca ctgttcacgg taaacagctg tcatgaggat ttgccctacc ccattggttg
    25681 catctgtgat tgaatcacga tagggacgat tggcaccaat agactgataa atgaagccaa
    25741 aggccaacca ccaaagcaca ctaatggaaa gatactttgt ccagcccttg ccgtccagtt
    25801 tacgcgcgac aaactcctgc ttttccttct taaactgtgc ctgataaatc caaactagaa
    25861 gtccaattgg ctgcatgact gtgaagtaaa gtgtcgtcag cacctcacca taaaagcctt
    25921 tctgtagggc caaaataagg taaataacag agttaatcaa gccaaaaaga taattacttg
    25981 ctcgaccttc cgatacaaag attacacaga taatccctgt caagctacaa atcatcccaa
    26041 tccagtcaac aatacgatgt tcgtaaacca actccagcca gagaggaaaa cttcctaaaa
    26101 ccagcaaata aatccactgg gcaaaactac gatgggcaaa gaggtcatcc cagatagcct
    26161 tcatagttcc tgaaaatcct aaatcagcca tagccgcaac catacgacgg taaccacctg
    26221 acatttcacc tagggttgtt ttgatatttt caattttctt ttgcaaataa gtatgcatca
    26281 tttctccttt tgtttttaaa gagccgtgtc tggatagact ttcggacgca acgctctatt
    26341 agataatgaa ctgcctatac acaagatttc taaccttagt cgacatgagc tgaaacctct
    26401 tatttgttaa gtagttcaca aaatattata cacctatttt atgaatagtc aactgtcttt
    26461 acagtaaaat tttagaaaat catgaaaatt ttctctttct ttccatttta agtgacattc
    26521 agtcattctc acatcaaaaa agcccagacg aaattgtctg agcattcttt tatctagtcg
    26581 tttaaggaag ttgagttcag tatgtttaaa gtctctgtcc catcatttct tcaacaaacc
    26641 ttgttcttgg agaaactcct tggctacttg ctttgctgac ttgccttcaa caccgacttg
    26701 gtagttgagc tggctcatct ggctttctgt aatcttacca gccaatgtat taagaactct
    26761 ttccaactct gggtgtttct tgagaagagc ttctttcatg agtggagccc cttgataagg
    26821 tgggaagagt tgcttgtcat cttccaagac ctgtaaatca taacgctcca attccgcatc
    26881 agtcgaatag gcatccgtga tttgaatatc ccctgactga atagcctgat agcgaagggc
    26941 tggctccatg gtcgctacat tgagattgag accatacatt gattgcaagc ccttatttcc
    27001 atcttcacgg tcgttaaact cgagtgtaaa acctgccttc aactgccctt ccactttttt
    27061 caagtctgaa atggtcttca agccatattc ttgagcaatc tttttcggaa cagctacagc
    27121 ataggtgttt tgataagaca tgggtttgag ataggctaga tgatcctgct tagcaatgcc
    27181 atcacgcgcc acctgataaa cctgttctgg ttcatgactc accttgggtg atggttgaag
    27241 caaactttca gtcaccgtac cagtaaattc aggatagatg tcaatatcgc cttttttcag
    27301 agcttcataa aggaagcttg tcttcccaaa attcggttta acagtcgcag tcatgctggt
    27361 attttcttca atcagcaact tatacatatt ggccaaaatt tctggttctg gacctatttt
    27421 cccagcaata accaagtttt ccttctcttt ttgaaccaaa agagctggac tataagacag
    27481 acccagtaat aaagccacca aggcaaaacc tgagaaaatc gtccgtaatt ttgctttttc
    27541 catcactttt agtaggaagt taaaggcaat ggctagcact gcagaagaaa gtgccccaat
    27601 caaaatcaaa ctggcattat tacggtcaat tcccaaaaga ataaaggaac ctagtccccc
    27661 tgcaccaatc aaggccgcca aggttgccgt accgataatc aaaacagctg ccgtccgaat
    27721 cccagacatg ataacaggca tggcgagtgg aatttcaaat ttcttgagac gttcccatct
    27781 ggtcatccca aaggcaatcc cagcctcttg caggttcgga tcaattccct tcagcccagt
    27841 gatagtattt tgcaaaatag ggaaaatcgc ataaatcact agagctgtca aagccggcaa
    27901 ggtcccaatt cccatcaaag ggataaagag ccccaacaag gccagagacg ggatggtctg
    27961 gaaaatacct gcaatctgca agacccagtc ggccagcttc tcatgatagc gaagaaaaac
    28021 agccaaggga atcgcaagca aaatagctag taacaaggtc aaaagcgaca actgcaaatg
    28081 ttgagataga gctgtcaacc aatcactaaa acgatcctga aaagttgcaa ttaaattagt
    28141 catgaacact acctccaaac aagtctgcta caaagtctgt tgcaggcgct tttaaaattg
    28201 tctcgggatt cgctacctgg cgaatttctc catcctgcaa gacagcaata cggtccgcca
    28261 acttcaaggc ttcatccgta tcatgggtta caaaaatcgt tgtcatccca aactctttat
    28321 gcaattcttt tgtcagaacc tgcaactgtt ttctcgaaat agcatccaag gccgaaaagg
    28381 gttcatccat gaggaaaatc ttgggctgac caatcatagc tcggacaata ccgacccgtt
    28441 gctgttctcc accagataat tcactaggta agcgatgccc atactcggct actggtaaac
    28501 caaccttagc caaaagctct tctgttttct tcgtaatttc ttccttgctc caccccttca
    28561 tttcaggaat gagagcaata ttttccgcaa ctgttagatt tggaaaaaga gcaatagcct
    28621 gtaaaacata accagtagaa agacgaagtt cacgctcatc atagtctttg atgcgcttcc
    28681 catccatata aatatttcca tcagttggtt ccaaaagacg gttaatcatc ttgagcatgg
    28741 tcgtcttacc tgacccagaa ggccctacta aaaccataaa ttccccatcc tcaatctgta
    28801 agttgacatc tctcaagaca tccttttctg tgtagcgcag tgctacattt ttgtattcaa
    28861 tcattctttg tcctcaattt aaaacttccc tcgattggtc aagtcttcta ccttaggcat
    28921 aacttcctta ttatcccaat gctccacaat tttcccgttc tctaaacgga agatatcgta
    28981 ctgggcataa gcaacgccat caatctgagt ctgaccatag ctaaccacat agtttccttg
    29041 tcctaagagt tggaaaacaa agtcaaaagt gacactatat tcagccacat agtttttata
    29101 agcagcactt ccttgtccaa tatcatgatt atgctgaatc aaatcgtctg ccacataatc
    29161 actccactgc tctagctccc cattttggaa aatttctgtc aagaaacggc gaaccagctt
    29221 tttattttct gctttcttgt ccaaatcctt gatttcaaaa tctccaaaaa tttgatctag
    29281 ttggtcattt tcaggtgttc gatagtagtc aacgacatcc caatgctcaa caatacgacc
    29341 attctcatcc gcacggaaag tatccgtcgt cacccattga gcttctccac cattcagata
    29401 ttgatgaaca tgaacaaaga ccagattgcc atcctcaatg gtgcggacaa tcttaatctg
    29461 acgctctgga tgacgctcaa agaaatctgc aaagaaggct gcaaatcctt ctttcccgtc
    29521 aggaacacct gtcgaatgtt ggatataggt atcccctaca gactgggctt gagcctcagc
    29581 aactcgcccg tcttgaatgg catggatgta taggttgtga gcatttttca cttgttgtga
    29641 catattctaa acctcatttc ccttctcttt cagattcgcc aaaattcttt cttgaaaacc
    29701 ttcaaattgg tgaatttctt cctctgaaaa tcctttgtaa aagatagtat ccaatttctg
    29761 actgacacga tgccccactt ctttctggaa cttgcctaac tccgttaaaa ctaaatactt
    29821 cttacgcttg tcttttccac acggactaac aattacaagc ttttgttcct ctagcttttt
    29881 tatcatagtc gtcagcgtat tattcgcaag cccagtcgca agcgcgatat ctgtcgcagt
    29941 tgcgcagcca gtttcactat tccataaaac cgctaaaatc ttgccctgtt cacccctata
    30001 aagagcctca ggatcttgac tcagtaactt ttgaaaaatc cgcccattca acaaacgaat
    30061 atgatgggct agcaaatgac tatctttcat aacacctcca atttatttcg atatcgaaat
    30121 gaataaaata attgtaacac tcatcgttct aactgtcaac tatttcgatt tggaaataat
    30181 ttttgataat tatccacacc accatactcc ggctcaacta acttttaacg agagtttcta
    30241 aactccttcg tcctccagtc tacaaaagcc ttccattcgt actatcctat attttatgag
    30301 gggacacatt tttcctatca gaccatttat tttaaagata gaagtaaatc ataattgctt
    30361 ccatctgttc ttttatagta tattgaagtt agactagagc actgtatctt ctaaaacatt
    30421 gatagaaagc gatttgaatt tcccaatcaa tttgttcgta tttatagcat tttgaaacta
    30481 gaataggaca ccatgactgc taaaagattt ctataaattc atttgatttt cctaatcaat
    30541 ttgttcatat cttatttcat tccgctataa tttcacctta ccctatcttt ttcgtagcac
    30601 ccttcaaaca gcctatcccc taccgtttga cgattcctca cttcgctcca cttccattac
    30661 agaagtttct tcactactat gggctcggct gacttctcat gattccttgt tactactatt
    30721 tgaacgctca cgagatagat cttacaaaaa atgctttgat ccacaatgga atcaaagcat
    30781 tttaaagagt tcctcataca taagcgcaga agtcgcagtt cctctgtact tggcttcttc
    30841 tcttttgata aagcgagcca agttgagcaa ctcaggtgct ggatgtttgg gatttaggag
    30901 caattcacga ttgaccaggc ctgagagacg aactgcctgc aattgctcat ttgtagtagg
    30961 cagtttttta gcagtctcta ggagagcagc aactaaatct tcactcaaat catgtcgagc
    31021 atgattgtaa agatctttta taaggctttc taggtttggt tctgccatcc ctaccacctc
    31081 ccttatggtt taataatgtt taatcaaatc aaccgttgaa cgatccaatt tcttcaccaa
    31141 ggcttgtaag aaagcttgcg cttctaggaa gtcatccatt gcatagaggg tttggtgaga
    31201 atggatataa cgagcgcaga caccgatagt tgttgatggg acaccaccat ttttcagatg
    31261 agctgcacct gcatctgttc cgcctttacc acagtagtat tggtacttga taccagcttc
    31321 ttcagccgtt gtcaaaagaa aatccttcat ccctgggaga agcaagtgac ctggatcata
    31381 gaaacgaatc aaggttccat ctccaatctt gccttgacca ccgtagacat cacctgctgg
    31441 tgagcaatca actgcgagga agacttctgg gtcaaacttg gttgtagagg tatgagcgcc
    31501 acgcagacca acttcttctt ggacgttaga acccagatag agttcattgc cgagtttttg
    31561 acctgataaa gcttcagcta gctcgcttac catgaggaca ccgtagcggt tatcccaagc
    31621 ttttgagatg atattttttt cattggctgt caaaattgca gaactatctg gtacgatggt
    31681 atcaccagga cggatgccaa aactttctgc ctcagccttg tccgcaaaac caccatcaaa
    31741 aacgatatcg gcaatggctg gcatggttgg tccccccttt ccacgagtca aatgcggagg
    31801 aacagaacct gaaatcacag gaatttcatg accatcacga gtcaagagtt tgaaacgttg
    31861 gctgctaacc accatggggt tccagccacc gatttctacg acacggaagg taccatctgg
    31921 cttgatttcg ctgaccataa aaccaacttc gtccatatga gaagcgacca agacgcgcgg
    31981 tgcatccaca gcttctgaat gtttgatacc aaaaatacca cccaagccat ctgtcaccac
    32041 ttcatccaca tgcggtgtca acttttcacg aagataagca cggacaggcg cttcatgacc
    32101 tgagactgca gcaagttctg ttacttcttt aatttttgaa aataatgttg tcatttcagt
    32161 tccttctttc tttcatccat tttaccactt tttataggag aaggatagtg ggaaggtgga
    32221 tttctaagtt agtatcttag tcctgctcta tcttagaaaa ggatagtatt ctcttgcatg
    32281 tagtgcaaaa tctagtaaac attccaaaat taactcgaat atttatttcc aaacaaaaaa
    32341 acaatacacc atcaaagttg tttgtatttt tcatgaaatt tacagaaaat agttgacttc
    32401 cctttcttct ttctttaaat atatagttgg ttgagtttgg aatagtacgc tgtagctgct
    32461 gaaacatttc tagaaattaa tttgactttc ctaatagagt tgttcatatc ttatttcaat
    32521 ttactatagt acaaaactag aaaaggaaaa aatcatgacc aaaacaattc ttgttacagg
    32581 tgggactagc tacattggct cccataccgt taaagctctt ttaaatgctg gctataaggt
    32641 gcatgtcctc gataatctct ccacaggaaa tcgagcggct gtggatagtc gtgctagctt
    32701 taaagaactg gatgtttatg atgctagcgc cttaaaggct tacttagaag aaaatcagat
    32761 tgatgctgtc ctccattgtg caggtgaaat tgttgtgagc gaaagtattg aaaatccaag
    32821 taaatacttc actgccaacg ttgctggtat gaaccaagtt ctcaaagtct tatctgaagt
    32881 tggcattcaa aaaatcatgt tctcttcgac tgcttctctc tatggaaata actgtattga
    32941 caagccagtg actgaagata ccctgctcga ccctgtcaac ccttatgcag agacaaaact
    33001 gatgggcgaa cgaatgattt actggatggc caatcgctac gactggaaat atgttatttt
    33061 ccgttacttt aatgttgctg gggctgaaat ggatgcttca aacggtctgc gtgtgaaaaa
    33121 tccaactcac atcattccaa atatcaacaa gaccgcattg ggacaaaatg atagcttaaa
    33181 aatatttgga gatgactacg atacacgtga cggttcatgt attcgagatt acatttatgt
    33241 cttggacctt gcataggctc atgttaaagg aatgaactac ctatttcaag aagacagttc
    33301 ttctcaaatc tttaacttag gaactaaaaa aggctatact atcaaagaaa tctttaaaac
    33361 tgctgaagaa ttactaaatt aaaaaatccc acacgagatt gttgctcgac gtgctagtga
    33421 cccagccagt gtcctagcag atgcttcaaa agctaaacaa tatcttgatt ggacggctag
    33481 ctactccctc gaagatatta ttttatcaga ttaaggtaaa aacattttag aataggataa
    33541 caaaggagaa agtcttgttg ctctctcctt gttgtattta ttaaattgtc agaaatttta
    33601 ttgacagatt agaaaaataa tgttacattt atatccgcag atatctttcg ataccaaatc
    33661 tacatgaaag gacggggtat gaaactttct cattatttaa ttggcttact tctactccta
    33721 gtctttctct ctattagcat tgggaccagt gatttttcat ggggaaagct atttgatttc
    33781 gaccagcaga catggctcct ctttcaagag tcccgtctcc caagaactat cagtattctc
    33841 ctgactgcct ctagtatgag tatggcaggc cttctcatgc agactattac ccaaaatcag
    33901 tttgctgcac cgagtacagt tggaacgact gaagccgcca aactgggaat ggtgctgagc
    33961 ctttttgtct ttccatcggc tagtctgacc caaaagatgc tcttcgcttt tgtttcatcc
    34021 atcgtattca ccctcttctt cctagccttt atgaccattt ttactgtaaa ggaaaggtgg
    34081 atgttgcctc tgattgggat catctatagc gggattatcg gctcagtcac agaagttatc
    34141 gcctatcgtt tcaatctggt tcagagtatg actgcctgga cccagggctc cttctccatg
    34201 attcagaccc atcagtatga gtggctcttc ttaggcctca tcatcctgat aaccgtttgg
    34261 aaattatccc aaaccttcac catcatgaat ctggggaaag aaatcagcga gagtttgggg
    34321 atttcctact ccctacttga aaaactggcc ctctttctgg tggcgctaac gacaagcgtc
    34381 accatgatta ccgtgggtgg cctaccattt ctcggagtta tcgttcccaa tcttgttcgc
    34441 aagcgctatg gagataatct aagtcaaacc aaactcatgg tcgcactggt tggtgccaat
    34501 ctagttctgg cttgcgatat cctatcccga gttctgatta ggccctatga gttgtctgtc
    34561 agtctcttgc taggaatcat cggtagtctc ggctttatcc tacttctctg gagaggggga
    34621 cgaaaagatg cagactaaaa gcaaacatac caagctcttc tggattctca ttattcttgc
    34681 catcggagct tgtcttctct acttttggcc catcactcac ttgtcagcct ttgcttggaa
    34741 gttgcgttcc caaaagatca tcgtttatct cttggtagcc atcgcgactg ggatttcgac
    34801 cattagtttt caaaccctga cggaaaatcg cttcctgacg cctagtattt tgggaattga
    34861 atccttctac gtcctactac aaaccctact actggttttt gaaagcaagt ttcttcaact
    34921 tggcaaatcc cctatcttag aattcctagt cttacttctt gtccagtccc tcttctttct
    34981 caccttacaa ggttacttga agacactgat gaagcaagac ctggtcttca tcctgctgat
    35041 ctgtctagcg ctcagaagtc tctttcgaaa tatcagcacc ttccttcaag tcctaatgga
    35101 tccaaacgaa tacgataaac tgcaaaatag tctttttgcc tcctttcaac atctcaacac
    35161 ttccatccta gccatcggtt ctctgatcat cctcgctttg acaatctttt tctttcgaaa
    35221 agcagtcgtt ctagatgtct tgcacctgca aagagaaacg gctcagatat tgggactcga
    35281 tgttgaaaaa gaacagaaag agctcctctg gggaatcgtg cttttgacct caacggccac
    35341 tgccttggta ggacctatgg ccttcttcgg ctttatgctg gccaacctca cctacctgat
    35401 tgtcaaagac tatcagcaca agttactctt tatagtggcc attctggttg gatttattag
    35461 cttaaccttg gggcaagcct tgattgaacg agtctttgca ctggaaattc gtatcagtat
    35521 gatcattgag agtgtgggtg gcttcttatt ctttatctta ctatatagga ggtctcgtca
    35581 gtgaaactgg aaaacattga caaatccatt caaaaacagg atattttgca aggcatttcg
    35641 cttgaagtca gtcctcaaaa actgacagcc tttattggtc caaatggtgc tggaaaatcg
    35701 actctcctct ccatcatgag cagactaacc aagaaagatc agggagttct cagtatcaaa
    35761 ggacgtgaaa tcgagagctg gaattcgcaa gaactggccc aagaactaac catcctaaaa
    35821 cagaaaatca attaccaagc caaattgact gttgaagaac tggtcagttt tggacgtttt
    35881 ccctacagcc gaggtcgact tagatcagaa gactgggaaa aaatccgaga aactctgaac
    35941 tatttggaac tgaccaactt aaaagaccgc tacatcaata gcctgtcagg ggggcaactc
    36001 cagcgcgtct ttatcgctat ggtactggcc caggatacgg actttatctt gctggacgaa
    36061 ccactcaaca atctcgatat caagcaaagc gtcagcatga tgcagattct tcgacgactg
    36121 gtggaggaac tcggcaagac cattatcatc gtcctccacg atatcaacat ggccagtcag
    36181 tatgcagatg aaattgtcgc cttcaaggac ggccaggtct ttagcaaggg aagcaccgat
    36241 caaatcatgc aggctgacct gctcagtcaa ctttatgaga ttcccatcac gctagctgat
    36301 atcaatgaca aaaagatctg tatctatagc tagtaacata aaagctcaag ttagagaacc
    36361 ttcagtctct tagtcaataa gattaagaga ctccctaaat cgttatcaca ttttaaaaag
    36421 gagaaattat gaaaacatcc cttaaacttt atttcactgc cctagtggcc agcttcttgc
    36481 tcctacttgg tgcatgtagt acaaactcaa gcactagtca gacagagacc agtagctctg
    36541 ctccaacaga ggtaaccatt aaaagttcac tggacgaggt caaactttcc aaagttcctg
    36601 aaaagattgt gacctttgac ctcggcgctg cggatactat tcgcgcttta ggatttgaaa
    36661 aaaatatcgt cggaatgcct acaaaaactg ttccgactta tctaaaagac ctagtgggaa
    36721 ctgtcaaaaa tgttggttct atgaaagaac ctgatttaga agctatcgcc gcccttgagc
    36781 ctgatttgat tatcgcttca ccacgtacac aaaaattcgt agacaaattc aaagaaatcg
    36841 ccccaaccgt tctcttccaa gcaagcaagg acgactactg gacttctacc aaggctaata
    36901 tcgaatcctt agcaagtgcc ttcggcgaaa ctggtacaca gaaagccaag gaagaattgg
    36961 ccaagctaga caagagcatc caagaagtcg ctactaaaaa tgaaagctct gacaaaaaag
    37021 cccttgcgat cctccttaat gaaggaaaaa tggcagcctt tggtgccaaa tctcgtttct
    37081 ctttcttgta ccaaaccttg aaattcaaac caactgatac aaaatttgaa gactcacgcc
    37141 acggacaaga agtcagcttt gaaagtgtca aagaaatcaa ccctgccatc ctctttgtca
    37201 tcaaccgtac ccttgccatc ggtggggaca actctagcaa cgacggtgtc ctagaaaatg
    37261 cccttatcgc tgaaacacct gctgctaaaa atggtaagat tatccaacta acaccagacc
    37321 tctggtatct aagcggaggc ggacttgaat caacaaaact catgattgaa gacatacaaa
    37381 aagctttgaa ataagccact tcaaactcaa tttaatactc aatgaaaatc aaagagcaaa
    37441 ctaggaaact agccgcaggt tgctcaaaac actgttttga ggttgcagat gaaagctgac
    37501 gcggtttgaa gggattttcg aagagtataa aattttgaca ttctaaaacg catcctatca
    37561 agttcactca aacctgatag gatgcgtttt tatcattttg aaatctattt accccacctc
    37621 attccccttc ttgatttcgt ttaagggaaa agtggtctgg aacggggtcc ttacctgttt
    37681 tcgaccaggg atgacaacgt aaaatccgag ccaagcccat caataccccc ttaaaaccat
    37741 gtttttcaat agcctgaatc atgtagttgg aacaagtcag ctcaaagcga caagagggtg
    37801 gaaagactgg tgagataaaa cgttggtaaa agcgcacagg cgctattaaa attcgtttca
    37861 ttatttctta gttacagcca tggtgtgtag ttggctgatt tcttttttat taagacgacg
    37921 ggattctcct ggacggagtc ctgtcaagtc taggtgtccg aaacgagtcc gagacaactt
    37981 atctacttgg agaccaacag cttcaaacat ctttttaacc tgatggttac gcccttcatg
    38041 gatggtcaac tgcaccacag agcgattttt gactgggtcc actttgagaa tttcataaac
    38101 agctggcttg gttttcttac catcaatctc aagaccacgg gtcaaggggc ggagattgtc
    38161 cttattggcc acacccttaa cacgcgcgac ataaaccttg tcaatctcat tacgagggtg
    38221 aatcatctcg tctgtaaagt ccccatcatt ggtcaaaatc aagacacctg atgtatccca
    38281 gtccaaacga cccacagggt aaatacgctc tttgacattg ggcaagaggt cgacaaccgt
    38341 cttgcgaccc ttatcatctg tcacactgga aatcacaccg cgtggtttgt taagcagata
    38401 gtagaccttt tcttcgttgt agataggttg accttcaact tcgaccttgt cgcctgactt
    38461 gatagtggtt gctagttcac gcaccacttg gccgttaacc gtcaccaagc cttgcttaat
    38521 cagctcttct gcttttctcc tactggccac acctgcgtgg gcaatatact tattgattct
    38581 catcttcttc tatcctttca ccaaataatt ggctttcttg ggcttgaatc tcaagctcat
    38641 caatcactgg taattcttct aaatggttta tccccatgta atctaggaaa taatccgtag
    38701 tcacatagag gttggggcgc cccaatactt cctttttccc gtcttccttt atcaggtcaa
    38761 aagcctgcaa ctttgccaag gctccactcg agttaactcc acggatggca tcaatttcta
    38821 tccgcgtaat cggctgtttg taggcaataa tggacaaggt ctcaagggca gcccgagaca
    38881 agctctggtt gataggcgcc ttagagtatt ccttcaaaat ctctgcaaat tgagacttgg
    38941 tcaccaatct ataagcacca cttgtctcaa tcaaagccaa actggaatct gggtcctttt
    39001 catacttctg ggctaatttt cctaaactct gctggatgcc tgtcggtggc agagagagga
    39061 gttcagctaa ctggcggacc cgaatcccat cttcacccgc tacaaacaag agcgcttcta
    39121 tttttgctaa agtactcatc tttcctctct atcaagtcta gctttgaggc acttgacttt
    39181 cttccttctt ttccatgaga tagatatctc caaaactctc ctcttgcacg aggatcagct
    39241 cctgggtttt gattaactct agggttgcca aaaagagggt gatgacctct tggacattct
    39301 gggcttcctt gaacaaatcc tgcaagcgca attgatctcg tccaatcaag gactctttca
    39361 cgataatcat catgtcctca atcttatact catcccgcaa gatagtcgtg tgattttgtg
    39421 caaactcctc ttttttcttg gctaggatat ttgaaaaagc caaaaagagg tcaatggtcg
    39481 tcttgtcatg cacaagctcc gcatcttcgt aaatcaactc tgtcggcgct ttggaataat
    39541 actgggcccg ttcttggtgc ttggcttcca agtgctcacc caagagcttg aacttgcgat
    39601 attcttcgat ttgagagagg aggtcctgct ccaggtcatc ccccaagtct gtcacttctg
    39661 ctaccttcgg aaggagttta cgactcttaa tcagcatgag ctgactagcc atgaccatgt
    39721 actcacccgt cacttccaga cgcatggcct gcagggttga aacataggct agatactgtt
    39781 cgatgacttc cgtaatgggc acatcgtaga tatccatctg gtacttagaa accagatgca
    39841 agagcaagtc cagggggcct tcaaaatctt ttaatttaat atccattatc tatatttttc
    39901 taaggtcagg actgttttta atcctaattt ttttgcaatt tcgtacaaat cgaccttgtt
    39961 ttctatttgt cttagaataa actgttcacg taagacttga gctgataagg atggaaaacc
    40021 tttctccttg acaaaagatt ctaactgacg aaaggcccac tgacgagaat agggtttctc
    40081 tcctctttca aaaagatagg tctgccccat caagggttcc aattctgaaa gcaaggccgt
    40141 gggaatggtg acaatcctct gttgggaagc cttgctgatt cgcaacacct gaaaatccag
    40201 attgatgtcc gcaaccttga tggctaaaat ctcactgggc aagagcccca tttctaggat
    40261 taagagcgct agcaagcggc cctctggatg gtcgctttcc tgccaaaaag agtctaggta
    40321 tagaatctct ggcttttccg tcttcttttc agcttgtttg gctaattcca agcggtaaaa
    40381 gctgtccacc tctccttttt gatagagaaa gtatagaaat tggttacagg ccgaaatctt
    40441 tcgcttctgg gcgctgattt ttagattggc tagctgggct tggtaaatct tgagactggt
    40501 ctcagaaatc cgctcaccta ccatgtctaa aaattgctcc aaatcatact tataggactg
    40561 cttggaattg acagacaagc cctgcttttc ctctaaaaag gctgaaatcc tgtctctcat
    40621 tggcatcgaa tctcatattc cttactaaag tcatgcaaga gggcattaac ggccttgagg
    40681 atggacttac gcgtaataat cccttggaaa ataccctctg catccacaac cggtaagaag
    40741 gactcatcta ctagcttgtg caagacctcc gtaatggtga aatcaggcga aacaaccgct
    40801 acgtccgttt ttgtcatatg aacgatatcc gtatccgcca tgatttcttg gctcaagtca
    40861 tgctccatct gataagccat aatatctctg agtccaatcg tcccaacaaa ctgtttttca
    40921 tctgtcacaa cgggaacacg ggtataggtc atctgactga gcaagagggt cgcatgatcc
    40981 gcattgtggg tatcaatcaa cacagctaga tttttagcag gggtcaaaaa agtttcctcc
    41041 tgccccaaca agaaagtctc aaactccttg gcaatcatcg gctaaactcc ttggacaaac
    41101 ctggatacac ctcgtgatct cgtgtcaaaa agtccacttt gaagtaacta tcatcaatct
    41161 ccacacgagc atagagacat tctctgatgg tacctcgtgg ttgactgata gaacctggat
    41221 ttagaaagag gatcttgcct tccaaccaag cacttggcac atgcaagtga ccatagaggc
    41281 agatagcggc ctcttcctcc tgagcccagt agtccaactt ttgaaagttg aaattgatgt
    41341 caaacaagtg accatgagtt tggataatct tggtcgaacc aagctcagtc accagacgtt
    41401 ctgggtagcc ggcgtagaag tccatgtttc ctttaacaac gcggatgccc tcccaaagtg
    41461 gagaatccgg acgtagttca gaatcgccgt tatgaaaaac ggcatcgact ttgcccacat
    41521 agcgatcacg gacttcttcc acaatcaagc tatcgccatg ggaatcgctc attacaatga
    41581 tggtttgctt tgccatgatg gaaatacctc caaaagtttc ttaacggcta aggcacggtg
    41641 agattgacta tttttttctt ccagggttaa ttcagctgat gacttacctg tctctcctac
    41701 aaggaagagg ggatcatagc caaagccatt ttcaccctta ggttcaaagt taatataacc
    41761 tgaccagtct gcttcaacaa ctaaactttc cttatttggg ctggctacga ctagggttgt
    41821 gtggaactga gccgagcggt ccttgagttc aaagaccatg gccaattcgt gcaagagttt
    41881 ggcattattt tcacggtcag ttgctcccac acctgcgaaa cgagctgacc agacgcctgg
    41941 taagccacca aggacatcga ctttgagacc agaatcatct gccaaaacca tcttgcccgt
    42001 taattgagaa atggtttctg ccttaaggcg ggcattttct tcaaaggtca tacctgtttc
    42061 tgctacttca ggcaggtcag ggtagtcatt aagattttcc acatcgtagc ctaacttatc
    42121 aaagatagct cggaattcct tggttttgcc ttcgttacga gtcgcaatca acaaggtttc
    42181 tctgaccttg cttgtctcaa agaaatcatg aacattgacc ccttctttag gcaattctac
    42241 atacaagagt tgcccatttt caaccaagag cagggcccca tgacgttgaa taacttccaa
    42301 gtttcgtccc atttcttgat taagcatgtc taccacaaag gagaacaaac ggtaggtaga
    42361 accatagcgt aaaacagtaa ctgaaagcgg gaaaccatac tcttcatctc caaatatttt
    42421 ggagaattca aacagaggaa aatctgtctt atagtcgctc aaactgttaa cgccaccaaa
    42481 aatactataa gacccaacat accaatcctg gtcatcctta tattcataaa ttttatttgt
    42541 cataattcta catgctccac ataaatctct ttttccagcc attcttcacc aatttgtgca
    42601 aaactttggc tactggctgt tgtgtaaaaa cggtgatgga gtggtccagc atcgcgacca
    42661 cgattgattt caaaataatt gagtaagact gagatatccc gtacgcactc tgccccacta
    42721 tcgatgagct gaacctttgg ccccatgaca ttttggataa tagggcgaag gagtggataa
    42781 tgagtacagc ccaaaatcag gctatccacc tttccaacca agggacgcag ggtttcatag
    42841 accaccttct tggtaacact ggttgacagg gcacctgact caaccaaggg agcaaacttg
    42901 ggacaggcca agctctccac ctgtaagtcg ggatccagat catggatttt ctgacggtat
    42961 atgtctgatt gtaccgtcat gggcgttcca atcactccga ttttcccacc ttgactggac
    43021 ttgatggctg ccgaagctcc tggcaaaatt acacccaaga caggaatatc tagttgagcc
    43081 ttgatttctt cccagacgac cgcagtcgca gtgttacaag caatgacaat cattttgaca
    43141 tccttggtca agagaaagtt gaccagctgc caagtatatt cacgaatttg ctcagctgga
    43201 cgggggccat agggcgcccg cgccgaatct ccaatataga cgatttcttc atggggaagc
    43261 tggcgcatga gctcgcgcac aacggtcaag cccccgacac ccgaatccaa aaaaccaatt
    43321 ggtcgattat ccatacagtc ttctttctag tcttttttct gattgatcgt tcattatgat
    43381 tccttgtact gaacgaactg accgacagct tgatagaatg tcaactacct ctctcttaat
    43441 cataatcaaa tatcaataga atgcaaggaa aaagtctcat ccctaaacca taaccaacat
    43501 agtgagaagt ctggaacttt catcccagac ttttccttat ttattttttc tttttattgt
    43561 tagcaagggc tgccttttgt tggcggatga tttggtggta aacttgttgt accttagctt
    43621 cgcttggttt ttgaccattt gcactcaaaa gagtacgaac tgcttcagca ttcaaacgtg
    43681 ggttgtcagc aaattctttt tcgatttgct tacgaaccaa gtacattcct ccaagagctc
    43741 ctcctagaaa agctagcaca atcaatacaa ttgctaaaag taaatccatc atttttctcc
    43801 tgtttctttt tgagtccctt tcattatacc acaaactagc caccctgacc agtttgttct
    43861 acactttcag ggatggaata gacagcaaaa agaaccctgc ttttctgaaa aagaggtctc
    43921 cttgctaagt ttaatttaca taaatagcca gtgtttgata gggttttagt agaattttct
    43981 cagacacttc tgcatcttca tagtttgaaa tcaaaatctg tccattttgg tagactgctg
    44041 gcaagtcgat ttccacttct ttagcataaa agttattgag cactagtaac ttttgatcct
    44101 caaactggcg ttcaaaagcg tagacttgtt tgctatcttc aaaggctggt ttgtaacttc
    44161 cttctgaaat gattggcatt tccttacgca gtcgaatcaa gtcttgatag aaggtaaaaa
    44221 tcggaccctg gatttcattt tctacattga tgtctttata ggatttacca gctttcaacc
    44281 aaggagtgcc tgttgaaaat cctgcatttt ccgaagcatc ccactgcatg ggaatgcgtg
    44341 aattatcacg cgacttagct tgaataatct ggaaggcttc ttgctgactc tttccttctt
    44401 ctaggagcat ctgataggca ttaagcgatt cgacatccac gtaatcagcc atagaatcat
    44461 agtctgggtc aatcatcccg atttcctcac ccatgtagat ataaggtgtc ccacgtgaca
    44521 ggtgaatgct ggctgctagc atggtggctc cttccttgcg gaagttttga atatcgacaa
    44581 aacggttcaa ggcacgtggt tgatcgtgat tgttccaaaa gagggcactc caaccgtctt
    44641 tatcactcat ttccttaccc caactatggt aaagactctt caactcttca aaatcaaagg
    44701 gagccaaggt ccacttttgt ccatccttat agtccacctt gaggtgatga aaattaaagg
    44761 tcatggataa ttcctgacga tcaggcgacg aatagaggac acagttttcc atggtggtag
    44821 aagacatttc cccaactgtc ataaagctat cgtcagatcc aaaagtggct tggttcatca
    44881 tacgcaaata gttatgaacg atgggtttgt ctgtataagc tggcttccct tcattttcag
    44941 gacagtccac tgaaacctcg tccttaccaa tcaaattgat cacatcaaat cgaaaacctt
    45001 tgacaccctt gtcgcgccag aaattaacaa ccttgaaaag ctccttacgg acattgggat
    45061 tgcgccagtt aaggtcagcc tgggtctcat caaataggtg aagatagtat ttcccagtat
    45121 ccccgaaagg cgcccatgca gaaccaccaa acttagactg ccaatctgtt ggttggtctt
    45181 ggatgaagaa aaagtcttga taatacttat caccagctag ggctttctga aaccattcat
    45241 gctctgtcga acaatgatta agtaccatgt ccagcataaa gtcaatcttg tgctctttac
    45301 cgatacacac catttcctca aaatcagcca tatcaccaaa aagaggatcc actgccatat
    45361 aatctgaaat atcgtaacca ttatcccgtt gaggacttgg atagaatgga ttgagccaga
    45421 ccatatccac acctagtttg gctagatagg gaatttttcc gataatccca cggaaatccc
    45481 caataccatt gccagtggtg tctttgtaag attttggata gatttgatag actacttttc
    45541 ctttatcaag tgtcatctgt ttctcctttt ctgataaaag ggaggaagca gtcttccgtc
    45601 cctatttgtg ctatttcaat tatactcaat gaaaatcaaa gagcaaacta ggaagctagc
    45661 cacaggttgc tcaaaacact gttttgaggt tgcagataga gctgacgtgg tttgaagaga
    45721 ttttcgaaga gtattagatt cgtgtagcga ccatgagaga tgctccagct tgaatcgttg
    45781 tcggataagt tccgggaata gtcgctgtat aagcatcttg gttggtgatg ataacaggag
    45841 tttctgtcac cagacctgca gccttaatga catccatatc aaaacgaatc agttgctgac
    45901 caactgtaac gtgatctcct tggactacaa gactttcaaa acctttgcca tcaagaccta
    45961 ctgtatccat accgatgtgg atgagcaatt caactccctc gtcagagaca atgccgatgg
    46021 catgcttggt agggaaaaga accgtcactg tcccattaac tggagaggtc aactcacctt
    46081 ggcttggttc aatgactaga ccttgcccca tgacacctga tgcaaaaata ggatccgtcg
    46141 cttgactcaa ttctttcact tggccagtta gtgggctgat aatttctacc gaagtaagtt
    46201 ctactggttc atggttcaca aattctgctt cttcttgagc aacgaattct gcctgcaagt
    46261 tcgtatcgcc ctctgttttt gtaaagagac cagccttgcg gaagaagaaa gtcaagagca
    46321 ttggaacaac aatcgcaact agcatagttc ctgcaaatgg cagcatgtat tgaggttgaa
    46381 tagagagaat acctggcaaa ccaccgatac caatagaagc cgcagttaca ttaaaagtaa
    46441 cggataacat gcctgcaagg gctgaaccag tcatcccagc aacaaatgga taaatatatt
    46501 ttacgttaac cccaaaaaga gctggttctg taacaccgag ataggctgaa atggttgcag
    46561 gaagtgaaac ctgagcctca cgctcatcat ggcgatgcat gaaataatag gcaaacacgg
    46621 ctgagccttg agcaatatta gaaagagcaa tcattggcca tagggcagtg ccaccagcat
    46681 ccgcaatcaa ttgtgtatca atggcattgg tcatatggtg cagacctgtg atgacaaatg
    46741 gagcgtagag ggcaccaaaa attgcaccga agagccattt aactggacca gttaaacctg
    46801 ccaagacaac tgatgaaagt ccttgtccaa ttgtccaacc gattggtccc aaaacagtat
    46861 gagccaaaat caaggctgga atcaatgaca agaaaggtac aaaaatcata gaaatgactt
    46921 ctgggatatg cttgcgccag aagatttcaa gataagatag actcaaacct gcaagcaagg
    46981 ctgggataac ttgggcttgg taaccgatac gattaacagt aaaatagcca aaattccaaa
    47041 cccagtttgc cgcgatatca gctgctggcg ttgaagcaac cgcataggca ttgagcaact
    47101 gaggcgatac caaacagatt ccgagaacaa ttcccaaaat ttggctggtt cccatcttac
    47161 gagaaacaga ccaagtaatc cctactggta agaactggaa gatagcttca ccaggcaacc
    47221 agaggaaatg attaacacct gcccaaaact gagaggattc tgtgatggtc ttgccatcca
    47281 acatcgacca atggacacct tccaagacat tacggaaacc gaggatcaat cctccgacta
    47341 tcaaggctgg aataatcgga gtaaaaatct ccgccagagt ggtcatgaca cgttggacca
    47401 cgttttgatt actcttagct gcagacttgg ctgcttcttt ggaaacaccc tcaatacctg
    47461 aaacagctgt aaaatcatta taaaagatgg gcacgtcatt tccaatgatt acctgaaatt
    47521 gacctgcatt tgtaaaggtt cctttaacag ctggaattga ctcgatagct ttaacattag
    47581 ccttcttatc atttcctaaa acaaagcgca tccgtgttgc acagtgagtt acggcagtca
    47641 cattttcttt gcctccgatt gcctgaagca gatctttggc ttcttgttca aattttccca
    47701 tttgattctc cttatacttg tcaaagcaag tttgatttga taagtatatt gtaaacgata
    47761 ccaaacttgt ctgcaacttg tttggtataa aaagggtgtt tttttgtaaa ttttctagca
    47821 cttgtatggt aaaatagata caggtgttca ttaaactaga ctaaaaacct atttaagcag
    47881 gcaaaatgaa gaaataccaa caattattta agcaaatcca agaaaccatt caaaacgaga
    47941 cttacgctgt cggagatttc cttcctagcg agcacgacct tatggagcaa tatcaagtga
    48001 gtcgtgatac cgtccgaaag gccctgtctc tcctccaaga ggaaggattg atcaaaaaga
    48061 taagagggca aggttctcaa gtcgtcaaag aagaaaccgt caatttccct gtatccaacc
    48121 taaccagcta ccaagaacta gttaaagaac ttggactgcg ctctaaaacc aacgtggtca
    48181 gtctggacaa gattattatt gataaaaaat cctcactgat aaccggtttc ccagagtttc
    48241 ggatggtttg gaaggtggtc cgccagcgtg tggtggatga tctggtatcc gttctggata
    48301 cggactatct ggatatggaa ctcatcccaa atctcactcg ccaaattgct gagcagtcta
    48361 tctattctta tatagaaaat ggcctcaaac tccttattga ttatgctcag aaggaaatca
    48421 ccattgacca ctcaagcgac cgagacaaga ttctcatgga cattggcaaa gacccttatg
    48481 tcgtttcgat taaatcaaaa gtctatctcc aagacgggcg ccaatttcag tttaccgaaa
    48541 gtcgccataa gttagaaaaa tttagatttg tagattttgc aaaacgcaag aaataaaaga
    48601 ctgagacacc agatctcagc ctttttcttt atcccaaaac taattcatca ctgaccagac
    48661 tttggccact atttttctgg aagagatgca taaggtcttc aactgtcaga tgctttttct
    48721 cttctccctt gacatccacc actatcttgc cttgatagag cataatgaga cgattgccgt
    48781 actcaatcgc atgattcata tcatgggtaa tcatcaaagt cgtccactga tggtactcca
    48841 caatcttttg cgtcaagtcc atcaccattt gactggtttt cgggtcaagt gccgcagtat
    48901 gttcatccaa aagcaaaagt ttgagtttca ccagagctgc catgactagg gtcaaagcct
    48961 gtctttgtcc tcctgagaga tattgagtat ctacttttaa gcggttttca agaccaatat
    49021 tcaactcctt caaggcctct tggaactgga ttctatcctt ctccttcacg ccccaaccaa
    49081 gccctctctt ttgcccacgt ctcaaggcaa tagccatatt ctcctcaatc gtcaaacgag
    49141 aagctgttcc catcttagga tcttgaaaaa cacgggcaat atccttggct ctctttctga
    49201 cactcagatg tttaatggat ttccctgcca gtagaaggtc cccttcgtcc accgatagat
    49261 taccagccaa gatattcatc aaagtggatt ttcctgctcc atttccacca atcacagaga
    49321 taaaatctcc ctcttcaacc tctaaatcta atcctttgag gacatggttc tcattgaccg
    49381 tccctgcttc aaatgtcttg tgaatatttt caagtgttaa caaacttgcc ataactttct
    49441 cctcctattc atttctcaat ttcagaccac gaatctttaa tctcttttgc aatgctggtg
    49501 caaataaaac caaagcgagc aagattgcat taaagagacg taccaagttt tgatctagat
    49561 ttggaatttc atagatattt aaaataatca aacggtaaac aatagcaccg attccgatgg
    49621 ataataagcg gcctccaatg gtcaagtcgt gtatcaagac ttccgagctc aaaccaacaa
    49681 caatggttcc tgtcccagaa gtcacatctg aaaatccatc attttgggca aacaaggaac
    49741 cacatagggc aatcaaaccg gttgaaatca tgtaaccaac aatcttcatg gtgtttacat
    49801 tgactccatt ggcctcactc atcgggatat tgtccccagt cgaacgcaag accaaaccaa
    49861 tctctgtttt catcaaaagg gtcaaaacca aacaagtaag agacagacta aactaagtga
    49921 gaaaacagct ccttcatttg tcagacccaa gctcgccaac tgtttaaaga cggttgcaga
    49981 atctcccaag gaaagattgg gtacactgcc catgatttta atattgattg aatagagccc
    50041 tgtcaaggtc acaatccctg tcaagagagc tgggattttc atcttggtgt ggagcattcc
    50101 tgatataaga cctgctacca tacctgccag caaagcaagt aaagtcgcaa gccaaggatt
    50161 tgcccctgcc tgtatctgtg atacaacgat tgctgccccc ataggaaagg ctccctcagc
    50221 agtcatatcc gcaatatcca aaatacggaa agtcaagtag accccaattg ccataatcga
    50281 ccacaacaaa ccttctgata aactagataa cacaaaattc atcttaatcc tccttattcc
    50341 ttgccttcta acttactaat atcaattcac aatgcatctg ccatttcttg attagtatgc
    50401 aattccagtt tttcaggcaa ctcaactgct atattttctg gttcctcacc ttttaaaaca
    50461 cgaatcagca tgcgagctgt ctgtcttccc aattcttcat aattggttcc gtagttatac
    50521 aagccaccaa cagcaatcat ttctgttgaa ccaccgaata ctggaacctt gtgtttaata
    50581 gaaacctgct taactgtttc catggttgac gaaatgatat tatcagttgg gacaaaaacc
    50641 atatccacct ctgccatcaa actctctgct gccgccttga cgttattact gtccaagatt
    50701 gttttttcaa caacggtaaa gcctttttct ttcagaaatc gcttagcttc atccttttaa
    50761 acaaccgaat tgggctcgct ctgagtatag aggattccaa tcgttttagc ctttagcaac
    50821 actttcttta tcaaattgat ttgggttgaa atggcatctg atgactgatc gcttgtccca
    50881 gttacattgc ccccaggatg ttctcttgac tcaaccaact tggcactgac tgggtctgtt
    50941 acagctgaaa agataactgg tgtcgtttgc gtcgtattgg ccaagctctg agcagaaggc
    51001 cttgcgatgg ctagaacgac atcgctggat tctgctagtt gctgggaaat cgtttttaga
    51061 tttctttgtt ctccctgtgc attttgcaaa tcaatctcaa tattttcccc ctcaacatag
    51121 ccttgtttgg ccagctcatc cacaaatcct tctctagtag cgtctaagga ctggtgggta
    51181 ataaactgcg aaataccgat acgaaagaca tctcttttct tatctgcctt caactgatgc
    51241 aaactgatca gagaagtcaa gaggatcccc actaccaaga gggggctagt gattttcgaa
    51301 caactttcat aatgaaactc cttctcagaa aagataaaac aaaaaaacgc ctagatacta
    51361 tctaaacggg tacttgaaat aacctaacaa gttacaactt agctggtcca tttagatatt
    51421 catctatatg gaccacaatc aaaaatctta gccacataga tacaaacaaa ttgcttgaac
    51481 tgtttgcatc catggtgaca tgtctacaaa cactatctaa agtagctaaa gtaaaagttt
    51541 tgattcatag ttacggcttg tctcttattc atttcttttt ctgctttctt ttttgatcct
    51601 tcctatccta ccaccttttt catcacctgt caataatatt tcaaaatttt ttaaaatttt
    51661 tcgaaaattc tttcag
//
LOCUS       DAVBBS010000006        50102 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVBBS010000006 DAVBBS010000000
VERSION     DAVBBS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 50102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50102
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.006"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..746
                     /locus_tag="VQU32_000352"
     CDS             <1..746
                     /locus_tag="VQU32_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET2471872.1"
                     /translation="YELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTD
                     KKFVFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVY
                     IIGTMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAA
                     IENVQELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLK
                     KAFELTNNEQKDQAVADDNESDENDDADY"
     gene            733..2085
                     /locus_tag="VQU32_000353"
     CDS             733..2085
                     /locus_tag="VQU32_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HET2471873.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKKQKILETINQEIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEMGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSDYSAFCKMMENSEENFKAIIDEEVGRK
                     "
     gene            2260..3246
                     /locus_tag="VQU32_000354"
     CDS             2260..3246
                     /locus_tag="VQU32_000354"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HET2471874.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKQMHDQGLIASISVGVKDYEY
                     DFVSQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            3310..3381
                     /locus_tag="VQU32_000355"
     tRNA            3310..3381
                     /locus_tag="VQU32_000355"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3343..3345,aa:Arg,seq:ccg)
     gene            3564..4262
                     /locus_tag="VQU32_000356"
     CDS             3564..4262
                     /locus_tag="VQU32_000356"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET2471875.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            4253..7792
                     /gene="smc"
                     /locus_tag="VQU32_000357"
     CDS             4253..7792
                     /gene="smc"
                     /locus_tag="VQU32_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET2471876.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKSEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAITVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDAVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLRSEEAALKTLQDQMATLTERLEVIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQDRLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDFEQEEIQRLLQEKVDN
                     LEKVDTELLSQQAEEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRYLQSQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNIEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKDRFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            7789..8583
                     /locus_tag="VQU32_000358"
     CDS             7789..8583
                     /locus_tag="VQU32_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2471877.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGETWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            8583..9401
                     /locus_tag="VQU32_000359"
     CDS             8583..9401
                     /locus_tag="VQU32_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2471878.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKATLKAASDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGHEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            9405..10625
                     /gene="ftsY"
                     /locus_tag="VQU32_000360"
     CDS             9405..10625
                     /gene="ftsY"
                     /locus_tag="VQU32_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET2471879.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAVLEESQDSEPAVGVSQEEIEELPEEEKLEHEGTIEENNSEVLEAERPQTEETVQ
                     EKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEELLIMSDVGVQVASNLTEELR
                     YEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQDNLTVMLFVGVNGVGKTTS
                     IGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWGRRVDVPVVTGPEKADPASVVFDG
                     MERAVAEGIDILMIDTAGRLQNKDNLMTELEKIGRIIKRVVPEAPHETFLALDASTGQ
                     NALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREELNIPVKLIGFGEKIDDIGEF
                     NSENFMKGLLEGLI"
     gene            complement(10671..12158)
                     /locus_tag="VQU32_000361"
     CDS             complement(10671..12158)
                     /locus_tag="VQU32_000361"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET2471880.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYQSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPAIFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRSVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRFE"
     gene            complement(12307..13047)
                     /locus_tag="VQU32_000362"
     CDS             complement(12307..13047)
                     /locus_tag="VQU32_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2471881.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDNLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(13047..15212)
                     /locus_tag="VQU32_000363"
     CDS             complement(13047..15212)
                     /locus_tag="VQU32_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET2471882.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            15385..16338
                     /locus_tag="VQU32_000364"
     CDS             15385..16338
                     /locus_tag="VQU32_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2471883.1"
                     /translation="MKNKRIFKDFQASKMNLNLYTSPLLAFVFVFIGEFVAYTLYGIS
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENCLSNLLKGFSLGLALFLLTLLSLVVLGQYRLESIHLNPYYLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            16415..18403
                     /gene="uvrB"
                     /locus_tag="VQU32_000365"
     CDS             16415..18403
                     /gene="uvrB"
                     /locus_tag="VQU32_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HET2471884.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            18413..18976
                     /locus_tag="VQU32_000366"
     CDS             18413..18976
                     /locus_tag="VQU32_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2471885.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKTNDGYYQINGNS"
     gene            18942..19430
                     /locus_tag="VQU32_000367"
     CDS             18942..19430
                     /locus_tag="VQU32_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2471886.1"
                     /translation="MTVIIKSMETPEEIEGKPLVHWQTWREAYDGLLPAEFQETMTLE
                     RCRFFSQKYPENTLIAMDGMKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LIKAALIALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDEQEKILELGKPIKEKRMVFY
                     SK"
     gene            19503..19970
                     /locus_tag="VQU32_000368"
     CDS             19503..19970
                     /locus_tag="VQU32_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET2471887.1"
                     /translation="MSRAQLTILTNICLIEDLEAQRVVMQYRAPETNRWSGYAFPGGH
                     VENGESFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVVCYKATEFSGTLRS
                     SDEGEVSWVQKDQIPNLDLAYDMLPLMEMMEASDKSEFFYPRRTEDDWEKKIF"
     gene            complement(19967..20482)
                     /locus_tag="VQU32_000369"
     CDS             complement(19967..20482)
                     /locus_tag="VQU32_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HET2471888.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFKPLSELTDGVHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(20494..21021)
                     /locus_tag="VQU32_000370"
     CDS             complement(20494..21021)
                     /locus_tag="VQU32_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2471889.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGSLYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(21299..21862)
                     /locus_tag="VQU32_000371"
     CDS             complement(21299..21862)
                     /locus_tag="VQU32_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2471890.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(21846..22397)
                     /gene="coaC"
                     /locus_tag="VQU32_000372"
     CDS             complement(21846..22397)
                     /gene="coaC"
                     /locus_tag="VQU32_000372"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HET2471891.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(22409..23098)
                     /gene="coaB"
                     /locus_tag="VQU32_000373"
     CDS             complement(22409..23098)
                     /gene="coaB"
                     /locus_tag="VQU32_000373"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HET2471892.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            23328..24998
                     /locus_tag="VQU32_000374"
     CDS             23328..24998
                     /locus_tag="VQU32_000374"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET2471893.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELEIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNALSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            25326..26501
                     /gene="mutY"
                     /locus_tag="VQU32_000375"
     CDS             25326..26501
                     /gene="mutY"
                     /locus_tag="VQU32_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET2471894.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     LIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            26556..27260
                     /locus_tag="VQU32_000376"
     CDS             26556..27260
                     /locus_tag="VQU32_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2471895.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            27253..28602
                     /gene="vicK"
                     /locus_tag="VQU32_000377"
     CDS             27253..28602
                     /gene="vicK"
                     /locus_tag="VQU32_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HET2471896.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            28604..29413
                     /locus_tag="VQU32_000378"
     CDS             28604..29413
                     /locus_tag="VQU32_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET2471897.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            29504..29746
                     /locus_tag="VQU32_000379"
     CDS             29504..29746
                     /locus_tag="VQU32_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HET2471898.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            29748..30002
                     /locus_tag="VQU32_000380"
     CDS             29748..30002
                     /locus_tag="VQU32_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET2471899.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            30049..30822
                     /locus_tag="VQU32_000381"
     CDS             30049..30822
                     /locus_tag="VQU32_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HET2471900.1"
                     /translation="MKKKWFFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFTTKRKWRFILENIFDLLAILPLNAIFTVFRLGRIFRLAKLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPASIFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLKDELSSQRILLEKQSKKIEELHKMIQDLIEKT"
     gene            complement(31082..32068)
                     /locus_tag="VQU32_000382"
     CDS             complement(31082..32068)
                     /locus_tag="VQU32_000382"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HET2471901.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGLYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            32262..34730
                     /gene="gyrA"
                     /locus_tag="VQU32_000383"
     CDS             32262..34730
                     /gene="gyrA"
                     /locus_tag="VQU32_000383"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET2471902.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIALRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEE"
     gene            34730..35473
                     /gene="srtA"
                     /locus_tag="VQU32_000384"
     CDS             34730..35473
                     /gene="srtA"
                     /locus_tag="VQU32_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HET2471903.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
     gene            35717..36514
                     /locus_tag="VQU32_000385"
     CDS             35717..36514
                     /locus_tag="VQU32_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HET2471904.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            36662..36784
                     /locus_tag="VQU32_000386"
     CDS             36662..36784
                     /locus_tag="VQU32_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HET2471905.1"
                     /translation="MTCDFKFETLQLHAGQVVAPATKSRAVPIYQTTSFIFDDT"
     gene            36806..37846
                     /locus_tag="VQU32_000387"
     CDS             36806..37846
                     /locus_tag="VQU32_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HET2471906.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFVDVDNLEEVEAAIKDNTKLVLIETLG
                     NSLINIPDLEKLAEIAHKHQIPLVSDNTFAIPYLINVFSHGVDIAIHSATKFIGGHGT
                     TIGGIIVDSGRFDWTASGKFPQFVDEGPSCHNLSYTCDVGAAAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETSSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKDVGSIFTFHVKGGEEEARKVIDNLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            38017..39291
                     /locus_tag="VQU32_000388"
     CDS             38017..39291
                     /locus_tag="VQU32_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HET2471907.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKDFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            39301..40179
                     /gene="truB"
                     /locus_tag="VQU32_000389"
     CDS             39301..40179
                     /gene="truB"
                     /locus_tag="VQU32_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET2471908.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEIILGYSTTTEDASGEVVAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALTLEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            40447..40722
                     /locus_tag="VQU32_000390"
     CDS             40447..40722
                     /locus_tag="VQU32_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET2471909.1"
                     /translation="MEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQSVMANLSQGL
                     SLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            40830..41468
                     /gene="udk"
                     /locus_tag="VQU32_000391"
     CDS             40830..41468
                     /gene="udk"
                     /locus_tag="VQU32_000391"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HET2471910.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            41596..42936
                     /locus_tag="VQU32_000392"
     CDS             41596..42936
                     /locus_tag="VQU32_000392"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET2471911.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            42914..43126
                     /locus_tag="VQU32_000393"
     CDS             42914..43126
                     /locus_tag="VQU32_000393"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET2471912.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            43123..43998
                     /locus_tag="VQU32_000394"
     CDS             43123..43998
                     /locus_tag="VQU32_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET2471913.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLESLQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            43991..44806
                     /locus_tag="VQU32_000395"
     CDS             43991..44806
                     /locus_tag="VQU32_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET2471914.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHSVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            44799..45230
                     /locus_tag="VQU32_000396"
     CDS             44799..45230
                     /locus_tag="VQU32_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2471915.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            45237..46904
                     /gene="recN"
                     /locus_tag="VQU32_000397"
     CDS             45237..46904
                     /gene="recN"
                     /locus_tag="VQU32_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET2471916.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRTIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLDLNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNKMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            46906..47634
                     /locus_tag="VQU32_000398"
     CDS             46906..47634
                     /locus_tag="VQU32_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HET2471917.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYSRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            47713..49536
                     /gene="lepA"
                     /locus_tag="VQU32_000399"
     CDS             47713..49536
                     /gene="lepA"
                     /locus_tag="VQU32_000399"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HET2471918.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMELERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(49738..49818)
                     /locus_tag="VQU32_000400"
                     /pseudo
     CDS             complement(49738..49818)
                     /locus_tag="VQU32_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
BASE COUNT        15242 a         8948 c        11370 g        14542 t
ORIGIN      
        1 tttacgagtt gtataaagat aaaaattata ataagcaaga atactacaaa agtggtggaa
       61 aaactgtcct agaaacattg agaaagagat ttggtttgaa agactatgtt tccccaacag
      121 aaattgatac tgataagaaa ttcgttttca tcatcgatga aatcaatcgt ggggagattt
      181 ctaagatttt tggcgaactc tttttctcta tcgaccctgg ctatcgtggt gaaaaaggaa
      241 gtgtttctac ccaatatgca aatctacacg aaactgatga aaagttctat atccccgaaa
      301 atgtttacat catcggaact atgaatgata ttgatcgttc agtggatacc tttgattttg
      361 ctatgcgtcg tcgttttcgt tttgttgaag ttactgccga gggtcaagtt ggcatgttgg
      421 ataaagagct gaatatccat gcagaagaag caaaaattcg tctaagaaac ttgaacgctg
      481 ctatcgaaaa tgttcaggaa ttaaacagtc attatcatat tggaccaagt tattttctta
      541 agttgaagga tgtagatttt gactatgaat tactctggtc cgattatatt aagcctctcc
      601 tagaagacta cttgcgaggt tcttatgatg aggttgaaac tttggaaact ttgaaaaaag
      661 catttgaact gacaaataat gagcaaaaag atcaggcagt agctgatgac aatgaaagcg
      721 atgaaaacga tgatgcggat tactgataat caacacaaga ttattaaaga aaaatttgtt
      781 gaagaatatc ctaaactaag caatcttctt ttagacagaa ccttggaaag tctatcccaa
      841 gatgaacgta ttttcatttt tccaaatgat ttgactcata ctccagattt ggataaaaaa
      901 caaaagattt tggaaacgat caatcaggaa atcaagacag gaaatgtgat tggttttctt
      961 ggatatggtc aggaaagatt aacgatttcc tcacgatttt ctgatgagag taatgaccat
     1021 tttttgcatt atctcttaaa caaggttctt catatcaatc tcactagttt agatgttgct
     1081 ttgtctcgtg aagagaggct ttatcaactt ttgatgtatc tcttccccaa gtatctacaa
     1141 gctgctattc gaaaaggtct ttataaggaa tatcatcgat tttctcataa cgacagtcat
     1201 gttaagggag tgattgatgt aagaaaccat ctcaagaaaa atcttccttt cacgggaaat
     1261 attgcctaca caacgagaga gttcacctat gataatcccc tcatgcagtt ggtccgtcac
     1321 actattgaat acattaagaa tcagaaaagc attggtcaag gggtactaga taatctctca
     1381 actagtcgtg aaaacgtatc tgaaatcgtg cgtgtaacgc cctcttataa actagctgat
     1441 cgtgctaaga ttattcgggg aaatcaatct aaacctatac gtcatgcata ctttcacgag
     1501 tacagaaact tacaagaact ttgtctgatg atcctaaacc aagaaaagca cggtttaggg
     1561 tatcaagatc aaaaaatcta tggtattctc tttgatgttg cctggctttg ggaagagtat
     1621 gtttacacct tgttgccaaa aggttttgta catcccagaa ataaggataa gacggatgga
     1681 atttcagtat tttctgttgg gaaacgaaaa gtatatccag atttttatga cagagaacga
     1741 aagattgttc tagatgcaaa atataaaaaa ctggaattga ctgaaaaagg aatcaatcgt
     1801 gaggacttat tccagctgat ttcctattct tatattttaa aagctgagaa ggctggactt
     1861 gtttttccga gcaaggacaa ggttattgat aatgagatgg gaaacctagc aggttatgga
     1921 gctttgttga agaaatggtc tatccaaatt cctcagaagt cttcatctta tagtgcattt
     1981 tgtaaaatga tggaaaattc agattatagt gcattttgta aaatgatgga aaattcagaa
     2041 gaaaatttta aagcgattat tgatgaagaa gtggggagaa aataatttcc cctctttctt
     2101 ttatttttaa cacaagtaaa acccgaacaa tatatttcaa ttcataaaat aatttgacaa
     2161 aaactgtact ttggtttata ataaattaag gaaacgtcgg aaaagacgta gtgatgcgag
     2221 agcataggta ggtcattaca aaagaaacga gacatcgata tgttaaatga atttccaatt
     2281 tttgattacg aagatattca attgattcca aataaatgtg tcattaaaag ccgtgcagaa
     2341 gcggatacaa gtgtcacttt aggaaatcac acctttaaac tacctgttgt gccagcgaat
     2401 atgcagacga ttttggatga aaatgtagca gagcaactgg ctaaaggtgg ttacttctac
     2461 attatgcacc gttttgatga ggcaggacgc attcctttta ttaaacaaat gcacgatcaa
     2521 gggctcattg cttccatctc tgtcggtgtt aaggactatg agtatgattt tgttagccag
     2581 ctcaagactg atgctcctga gtatatcact attgatattg ctcatggtca tgcggatagc
     2641 gtgatttcta tgattcaaca tatcaagaaa gaattgccag atacctttgt cattgctgga
     2701 aatgtgggaa caccagaagc tgtgcgtgaa ttggaaaatg ctggtgcgga tgctactaag
     2761 gtcggaatcg gtcccggtaa ggtttgtatc accaaggtta agactggttt tggtacaggt
     2821 ggttggcagt tggctgctct acgctggtgt gccaaggctg cgcgtaaacc gattatcgct
     2881 gatggaggaa ttcgtactca cggtgatatt gctaagtcta tccgcttcgg tgctagcatg
     2941 atcatgattg gttccctctt tgcaggacac attgaaagtc cagggaaaac gattgaagtc
     3001 gatggtgaac agttcaaaga atattatggt tcagcctcac aatatcaaaa aggtgcttac
     3061 aaaaatgtgg aaggcaaacg tatcttgctt cctgctaaag gtcatctgca agacacttta
     3121 actgagatgg aacaagacct tcaaagtgct atttcttatg caggtggacg tcaggttgct
     3181 gaccttaaac acgttgatta tgtgatcgtg aaaaactcca tctggaatgg ggatgcttcc
     3241 cactaagacg gggtctttga ccaaaaaact tgttattaga gcaaatttct gttataataa
     3301 aacaagtttc cacccttagt gtaatggata tcacgtaaga ttccggttct tgagatgggg
     3361 gttcgattcc ctcagggtgg atgtaaacat cctaaaaagc ctttaatagg gcttttttct
     3421 ttatcctgac ccaaatttgc cgctaaaact gagaatctct gtctaaagaa ttcccaaatg
     3481 ctttccagtc ccgttttaga gtgactcagg gggctttttt tgatataata aaaaggactg
     3541 ttatcagtta gaaagaggtt ggtatgaaag aattacaaac tgtactaaag aatcattttg
     3601 caatcgaatt tgcagacaaa aatttactgg aaactgcctt tactcatacg agttatgcca
     3661 atgagcaccg cctcttaaaa atttcacaca atgaacgctt ggaattttta ggagacgctg
     3721 ttctacagtt attgatttca gaatatctat ataaaaaata tcctaaaaag cctgaaggtg
     3781 acctatcaaa actccgtgct atgattgtcc gtgaggagag tttagctggt tttgcgcgtg
     3841 attgccagtt tgaccagttt atcaagttgg gtaaagggga agaaaaatca ggtggtcgca
     3901 atcgtgacac cattcttggt gatgcctttg aagcctttct tggtgccctt cttttggata
     3961 aggatgtggc caaggtcaag gaatttatct atcaagtcat gattcctaag gttgaagcag
     4021 gcgaatttga gatgattaca gactataaaa cccatctcca agagttactt caggtcaatg
     4081 gtgatgtggc tattcgttat caggtgattt ctgaaacagg gcctgctcac gataaggtct
     4141 ttgatgtaga agttcttgtt gaaggtaaga gcatcggtca aggccaaggt cgttctaaga
     4201 aattagcaga gcaggaagct gccaaaaatg ccgttgagaa ggggctggat tcatgtattt
     4261 aaaggaaatc gaaattcagg ggttcaagtc ttttgctgat aagaccaagg tcgtttttga
     4321 ccaaggtgtg acggcagttg ttggacccaa tggatctgga aagtccaata ttacagaaag
     4381 tctgcgttgg gctttggggg agtctagtgt caagagtctc cgtgggggca agatgccgga
     4441 tgtcatcttt gctggaacag aaagtcgcaa accgctcaat tatgcttctg tagttgtgac
     4501 tctggataat catgacggat ttatcaagga tgcaggtcaa gaaatcaggg tggaacgcca
     4561 tatctatcgt agtggagata gcgaatacaa gattgacggc aagaaagtcc gtctgcgtga
     4621 tattcatgac cttttcttgg atacaggttt gggacgagat tccttctcta ttatttccca
     4681 agggaaggtt gaggagattt ttaattccaa gtctgaggaa cgacgagcta tttttgaaga
     4741 agctgctgga gttttaaaat acaagactcg cagaaaagaa accgagagta aactgcagca
     4801 aactcaggat aatctggacc gcttagagga cattatctac gagttggata atcaaatcaa
     4861 gcctcttgag aagcaagctg agaatgcccg taaatttcta gacttagaag gtcaacgcaa
     4921 ggctatttac ttggatgtat tggttgctca aatcaaggaa aataaggcag aactagagtc
     4981 gacagaagaa gagttggctc aggttcaaga actcttgatg agttattacc aaaagcgtga
     5041 aaaattagaa gaagaaaatc aaactcttaa aaagcaacgc caagatttac aggctgaaat
     5101 ggccaaagac caaggcagtt tgatggactt gactagtctg attagtgatt tagaaagaaa
     5161 attagcccta tcgaaactgg agtctgagca agtggccttg aatcaacagg aggcacaagc
     5221 tcgtttggct gctttggagg ataagagaaa ttcactcagc aaagaaaagt ctgataaaga
     5281 aagctcttta gctctgttag agggaaatct agtccaaaat aatcaaaaac tcaatcgttt
     5341 agaagctgaa ttgctggctt tctcagacga tcctgatcag atgattgagc tcttacgtga
     5401 acgctttgta gctcttttac aagaagaagc ggatgtctca aaccagttga cccgtattga
     5461 gaatgagttg gaaaatagtc gtcagctttc tcaaaaacaa gcagatcaac tagaaaagct
     5521 gaaagagcaa ttagctatag ctaaagagaa ggctagtcag caaaaggaag agcttgaaac
     5581 tgccaaggag caggttcaga aattattggc tgactaccaa gctattgcca aggagcaaga
     5641 ggagcagaaa acttcctatc aagctcaaca aagccaactc tttgaccgtc tggataatct
     5701 caaaaacaag caggccagag ctcaaagttt ggaaaatatc ctgagaaatc atagtaactt
     5761 ttatgcaggt gttaagagtg ttctccaaga aaaagatcgc ctaggtggga ttattggtgc
     5821 agtcagtgag catctgacct ttgatatgca ttatcaaact gccctagaaa ttgcgcttgg
     5881 agcgagtagc caacatatca tcgtagaaga tgaagagtcg gcaaccaagg ctattgactt
     5941 ccttaaacgg aacagagccg gtcgtgcaac ctttcttcct ttgaccacta tcaaggcgcg
     6001 tacgatttct agtcagaacc aagatgctat cactgtaagc ccaggtttcc ttgggatggc
     6061 agatgagttg gtgacttttg atactagact ggaagccatt ttcaagaact tgctagctac
     6121 gacggctatc tttgatgccg tagaacatgc gcgtgcagca gctcgccaag ttcgttatca
     6181 ggttcgtatg gtgacattgg atgggacaga attacgcacg ggtggttcct atgcgggtgg
     6241 tgccaatcgc caaaataaca gtattttcat caagccagaa ctggagcaat tacaaaaaga
     6301 aattgctgca gatgaagcaa gcttgcgttc agaagaagcg gctttgaaga ccttacaaga
     6361 ccagatggct acattgacag aaagattaga agtcatcaaa tctcaaggag aacaggcacg
     6421 tattcaggag caaggcttgt ccctcgctta tcagcaaact agtcagcaag ttgaagaact
     6481 ggaaactctt tggaaactcc aagaagagga aatagatcgt ctttccgagg gagattggca
     6541 agcggataag gaaaaatgcc aagatcgtct tgctgcaatc gccagtgaca agcaaaatct
     6601 ggaagctgag attgaagaga ttaagtctaa caaaaatgcc atccaagaac gctatcaaaa
     6661 cttgcaggaa gagctagcgc aagctcgttt gcttaagaca gaactgcaag ggcaaaaacg
     6721 ttatgaagtt gctgatattg aacgcttagg caaggaattg gacaatcttg attttgaaca
     6781 agaggaaatc cagcgccttc ttcaagaaaa ggttgataat cttgagaagg ttgatacaga
     6841 attgctcagt caacaggcgg aagaagccaa aactcagaaa acaaatctcc aacaaggttt
     6901 gattcgcaag cagtttgagt tggatgatat tgaaggtcaa ctggatgata ttgctagtca
     6961 tttggatcag gctcgccaac agaatgaaga gtggattcgc aagcaaacac gtgctgaagc
     7021 caagaaagaa aaggtcagcg agcgcttgcg ctatttacaa agtcaattaa cagaccaata
     7081 tcagattagc tatactgaag ctctagaaaa ggcgcatgaa ttggaaaacc tcaatctggc
     7141 agagcaagag gtgcaggatt tagagaaagc tattcgctca ctggggcctg tcaatataga
     7201 agctattgac cagtacgaag aagttcacaa tcgtctggat ttcctaaata gccaacgaga
     7261 tgatattttg tcagcgaaaa atctgctcct tgagaccatt acagagatga atgatgaggt
     7321 caaagatcgc tttaaatcaa cctttgaggc cattcgtgaa tcctttaaag tgaccttcaa
     7381 acagatgttt ggaggaggtc aggcagactt aatcttgact gagggagact tgttgacagc
     7441 tggggttgag atttctgttc aacctccagg taagaaaatc cagtcgctta acctcatgag
     7501 tggtggtgaa aaagccttat cagctcttgc tttgcttttt tctatcattc gagttaagac
     7561 tatcccattt gtcatcttgg atgaggtaga ggccgcgctg gacgaagcca atgttaaacg
     7621 ttttggggat tacctcaatc gatttgacaa ggacagccag tttatcgtcg taacccaccg
     7681 taagggaacc atggcagcgg ctgattctat ctatggagtg accatgcaag aatcgggggt
     7741 ctcaaaaatt gtttcggtta agttaaaaga tttagaaagt attgaaggat gacaattaaa
     7801 ctagtagcaa cggatatgga cggaaccttc ctagatggga atggacgctt tgatatggat
     7861 cgtctcaagt ctctcttggc ttcctacaag gaaaaaggga tttactttgc ggtggcttcg
     7921 ggtcggggat ttctgtctct ggaaaaatta tttgctggtg ttcgtgatga cattattttc
     7981 atcgcggaaa atggcagttt ggtagagtat caaggtcagg acttgtatga agcgactatg
     8041 tctcgtgact tttatctggc aacttttgaa aagctgaaaa cgtcacctta tgtagatatc
     8101 aataaactgc tcttgacggg taagaagggt tcatatgttc tagatacggt tgatgagacc
     8161 tatttgaaag tgagtcagca ttataatgaa aatatccaaa aagtagcgag tttggaagat
     8221 atcacagatg acattttcaa atttacaacc aacttcacag aagaaacgct ggaagctgga
     8281 gaaacttggg taaacgataa tgtccctggt gtcaaggcta tgacaactgg ctttgaatct
     8341 attgatattg ttctggacta tgtcgataag ggagtggcta ttgttgaatt agctaaaaaa
     8401 cttggtatca caatggatca ggtcatggct tttggagaca atcttaatga cttacatatg
     8461 atgcaggttg tgggacatcc tgtagctcct gaaaatgcac gacctgaaat tttagaatta
     8521 gcaaagactg tgattggtca ccataaggac cagtcggtta tagcttatat ggagggctta
     8581 taatggcaga tataaaatta atcgcattgg acttggatgg taccttgctg actactgata
     8641 aaaggctgac ggatcgcacc aaggcaactt tgaaagctgc gagtgatcgt ggtatcaagg
     8701 tcgtattgac aactggtcgt cccttaaaag ccatggactt ctttctccat gagttaggga
     8761 ctgacggcca tgaagatgag tataccatta cttttaatgg tggattagtt cagaaaaata
     8821 caggagaaat ccttgataaa acagtctttt catatgatga tgtggcacgt ttgtatgagg
     8881 aaacagagaa attatcactg cctcttgatg ccatctcaga aggaacagtt tatcaaatcc
     8941 aatcggacca agaaagtctt tatgccaaat tcaatccagc tttgactttt gttccagttg
     9001 actttgaaga cctgtctagt caaatgacct acaacaaatg cgtgactgcc tttgctcaag
     9061 aacccttgga tgcagccatt cagaagattt ctccagaatt gtttgaccaa tatgaaatct
     9121 ttaaatcacg tgaaatgttg ctagaatggt caccaaagaa tgttcataaa gcaacaggtt
     9181 tggcaaaact aattagccat cttggaattg atcaaagtca agtgatggcc tgtggtgacg
     9241 aggccaatga cctctctatg attgaatggg cagggctcgg tgttgccatg caaaacgctg
     9301 ttcctgaagt aaaggcagcc gcaaatgtag tgacgccgat gaccaacgat gaggaagctg
     9361 tcgcctgggc tatcgaagaa tatgtgctaa aggagaacta agatatggga ttatttgacc
     9421 gtctattcgg aaaaaaagaa gaacctaaaa tcgaagaagt tgtaaaagaa gctctggaaa
     9481 atcttgattt gtctgaagat gttgatccta ccttcacaga agttgaagaa gtttctcaag
     9541 aagaagctgt gcttgaagaa agtcaggatt cagagccagc tgtaggggtt tctcaagaag
     9601 aaatagaaga attaccagag gaagagaagc ttgagcacga aggaactata gaagaaaata
     9661 attctgaagt tcttgaagca gaaaggcctc aaacagaaga aactgttcag gaaaaatatg
     9721 accgcagtct taagaaaact cgcacaggtt tcggtgcccg cttgaatgcc ttctttgcta
     9781 acttccgctc tgttgacgaa gaatttttcg aggaactgga agaactgttg attatgagtg
     9841 atgttggtgt ccaagtcgct tctaacttaa cggaggaact acgttacgaa gccaagcttg
     9901 aaaatgccaa gaaacctgat gcacttcgtc gtgtcatcat tgagaaattg gttgagcttt
     9961 atgaaaagga tggtagctac gatgaaagca tccacttcca agataacttg acagttatgc
    10021 tctttgttgg tgtgaatggt gttgggaaaa caacttctat cggaaaacta gcccaccgct
    10081 acaaacgagc tggtaagaag gtcatgctgg ttgcagcaga taccttccgt gcgggtgcag
    10141 tagctcagct agctgaatgg ggccgacgag tagatgttcc agtagtaact ggacctgaaa
    10201 aagctgatcc agccagcgtg gtctttgatg gtatggaacg tgccgtggct gaaggtatcg
    10261 atattctcat gattgatact gctggtcgtc tgcaaaataa ggataacctt atgactgagt
    10321 tggaaaagat tggtcgtatt atcaaacgtg ttgtgccaga agcaccacat gaaaccttct
    10381 tggcacttga tgcatcaaca ggtcaaaatg ccctagtaca ggccaaagaa ttttcgaaaa
    10441 tcacaccttt aacgggaatt gttttgacta agattgatgg aactgctcga ggaggtgtgg
    10501 ttctagccat tcgtgaagaa ctcaatattc ctgtaaaatt gattggtttt ggtgaaaaaa
    10561 tcgatgatat tggagagttt aactcagaaa acttcatgaa aggtctcttg gaaggtttaa
    10621 tctaatcaga agcaaaaatc ctgcaaggca taaacttgca ggaaattttt ttattcgaaa
    10681 cgaccatctt gacgataggc gatatctggt tgccaagtcc atttggcacc gaatttttca
    10741 agtaggtcaa agctggcttg aggtcccatg cttccagctt tatagtcatg aagtggggca
    10801 ccattttcag cccagagctc ttcaatacgg tcaatcaact tccatgatgc accaacttca
    10861 tcccagtggc taaagttagt tgagttgtta tttaggacat cataaatcaa tttctcgtat
    10921 ggttctggag aagcaccagt tgcagtcgca tctgtacggt aatcaagtga gttaggagcc
    10981 aagttaaatt cttctcctac ttgcttccca tttaggctaa gagagaagcc ttctgttggt
    11041 tgaatataga tggtcaaaat atttggagca agtggttctc caaagataga atccatttgt
    11101 ttaaagacga tgttgacatg agttcctttt tcagtcagtc gtttacctgt acggaaaaag
    11161 aaaggaacac tacggaatcg atcgctgtct acaaagaagg caccagatgt aaaggtttca
    11221 gttgttgatt ctggattcac atttggctcg ctacgataag agatgtattt catgccatca
    11281 atcttaccag agcggtattg cccacggata aagtgttctt tgagttcttc atcagttgga
    11341 tgatagaggt ttttaaagac cttaatcttt tcagcacgaa tctcgtcttt tgtgaagatt
    11401 gctggtttgt ccatggcgag gagcgaaaga agttgtagag tgtggttttg gaccatgtca
    11461 cggagggcac cggattggtc atagtagcca ccacgttctt ctacacccaa gcgctccgca
    11521 aaggtaattt gaacattgtc gataaaatcc ttgttccaaa cgttttcaaa aatcaagttt
    11581 gcaaagcgaa ctgcaaagat gctttggatc atttccttac caagataatg gtcgatacgg
    11641 aaaatttgtt cttcgtcaaa tgttgctagg agttcgtcat tcaacttgct tgcagttgcg
    11701 taatctgtac caaatggttt ttcaacgatc aagcgctcaa aacctttgcc atcgacaatg
    11761 ttttcagatt tgaggtgttt ggcaatggtt ccaaagaact gaggtgccat agacaagaag
    11821 aagagcttat tgtgttcagc ttggtatttt tcattaagct cagcttgtaa ttgacgcaaa
    11881 gcaatataat gttccgaatc attgacatca tggctttgat agtagaagtg gctagcaaat
    11941 tcttgggctt gctcggtact atctgccaaa tcaaggatgg actcgacaac tacagattca
    12001 aaatattcct tactccaagg tctacgggca gttccaataa cggcaaagtg cttggaaaga
    12061 ttgccggatt gatatagtct aaaaagggaa gggtagagct tgcgtttagc caggtctcca
    12121 ctcgcaccga aaattgtaac aataacctta gatgacatct agctacctaa tttctatttt
    12181 tattcctagt cttgctaggt atgaggaaac ctcatttcat aaacttaatt ctaacataat
    12241 tttccgaaaa atcaaaaatt ccaatacgag gtagaaaaac tgcaaggaaa tccttacagt
    12301 ttgagtttag acgtttaaga ccttatctaa gaactctttc agacgagggt gttgtgggtt
    12361 atcaaagatt tggtcaggtg ttccgtcttc aaggaactcg ccatctgcag taaagataac
    12421 gcggttggca acctgacgag caaatcccat ctcatgggtt acgataatca tggtcatgcc
    12481 ttgctcagcc aattccttca taacgttaag tacgtctcca accatctcag ggtcaagggc
    12541 agaagttggt tcatcgaaga gcatgatgtc tggattcatt gctaggccac gagcgatggc
    12601 cacacgttgt ttttgaccac ctgataggtt atctggattg gcattagctt tatctgctag
    12661 tccaaccttt tcaagcaact ccattcccaa ttcctcagct tcttccttag tcatcaactt
    12721 gtgctcaata ggagcaaagg tgatgttgtc caatacagac atatgaggga agaggttgaa
    12781 gtgttggaat accatgccga tattttcacg gacgtggtca acattggttg ttttttcagt
    12841 taaatcatag ccgttcacag tgatgtgacc gctagtgact tcttctaaaa gattgaggct
    12901 acggaggaaa gttgacttac cagaacctga aggaccgatg atacaaacaa catctccttc
    12961 atagaactta gtcgtaattc cttttaggac ttcatttttt ccatagtgct tgtgtaaatc
    13021 atttacatca atttttaatt ttgccattaa cgaatcctct tttctaagcg tttcgctagt
    13081 ctagtcaaaa gtgtgataat tacaagatag aagatagcaa ggattgcata catcttgaaa
    13141 ctttggtagt tacgagcaat gataatctta ccagtttgga agagttcaac caaaccgata
    13201 gcagatacga tagttgtatc tttaagagcg ataacgaatt ggttgacaaa gtttggcaac
    13261 atcaatttag ttgcttgtgg caagataatc ttacgcatgg tttttccata agagataccc
    13321 aagcttcggc tggcttccat ttggccaact ggaacggcct gaataccacc acgaacgatt
    13381 tcagcgatat aagcagccgc attgagtgag agggcaatgg ttccagctac aaagtcgtta
    13441 attgggcttt gttggcctgt gatagactcg atgaagtttg gaattcccca gaagatgaag
    13501 gctgcaagaa tcatcaatgg aataccacga ataacgtcaa cgaaaatctc agagatgacg
    13561 cgaagagatt tgtatgggct aacgctaaac ataccgaaga taattccgat gacaatggca
    13621 atagcaaatg agataagagc tagagcaaga gtgataccaa gaccgctaag gagttgtttg
    13681 tagttgtttt gaagcaagcc ccagagcgtt gtttcgtcaa cagtacttgt tgaagcagtt
    13741 gaagattcgc tagctaggta tttgtcaaga atcttttgga attcaccgtt tgctttaagg
    13801 tttgcaagtc cgttgttgaa catttcaatc agttctggat ttgctccttt tttaacggca
    13861 aaggctgttt caccgattgg agttccagag attggagttt tcaatttttg accttggctg
    13921 atagaatatt tgagaacagg ttcatcatcc ataacggcat caatggcacc agtgtttaaa
    13981 ctgtcataca ttgaagaacc atcagcaaag gttttgattt tgtaaccgta tttgctttga
    14041 ttttctgtta ggaaggtttg agaagcagtt ccgtttttaa caccgactgt ctttcctttt
    14101 agatcttcat aagaagcaat attgcttgat tctttgacac caagaatggt attagcagtg
    14161 tagtatgatt ctgagaagtc aaaagttgcc ttacgagcat ctgtgacaga cataccagca
    14221 atgataccat cggcttgacc agcttggaca gcactgatag cagcatcaaa accagggttg
    14281 gtgatttcaa tttcaaaacc ttggtcttta gcgattgcct taatcaattc catatcaata
    14341 ccagtgtatt ggttgcttga attttggaaa acaaaaggtg caaaagaaga atcgctggca
    14401 atgatatatt tagccttaac aggaatagct tttaatcctg cgagagtagt tgtagttggc
    14461 actgctgaag atgatgaagc agtccatttc ttgataattt tatcaagact accatctttt
    14521 ttcatttcag ccaaggcttg gttaaattca gtaaccaggt gctcgtattt acttcctttt
    14581 ttcacaccga aagcaaaact tcctacagct tcaccatcca tttcaatatg gaggtcttga
    14641 ccttggttaa tggcatattc gataacaggt ttgtcatcca tcatggcatc gatggcacca
    14701 gcactcaagc tgttgttcat taaatcacca gtgtcaaatg ttttaatagt aaagccgtat
    14761 ttatctttga ttgtttcaag gaaacgttga gcggcagttc cgtttttaac accaacggtt
    14821 ttgccagtta attggtcgta cttgctaatt ttgtgtgact ttgtagtagc aatgacaact
    14881 tttgtatcat agtaagtatc agacatggtg aagacttttt cacgttcttt agtctttgtc
    14941 atccctgcca tgatagcgtc ggcttgccca gcttgaaccg cattgactgc tgcgtcaaat
    15001 ccaggatagg acatctgaat gttccagcct ttaatctcag cgactttgtt aataatgtca
    15061 acatcaattc ctttataagt ttgatctgaa tctttaaact caaaaggtgc ataggcggta
    15121 tcagaaacaa tcttaatcgt ttctgctttg gcaataccta atgagaaaat tgggaataaa
    15181 attagcaaaa atgctagaaa ttttttcttc atttttagtc tccttttccg aatattctct
    15241 cattatatca gaaaaagtaa aaaaaggcaa atttttatat ttttcgtgtc agaaaatata
    15301 acattgattt ttccttttct ttcttgaatt gctattaaaa attgctataa taatactata
    15361 tatagaagaa aagaggacgg ggatatgaag aacaaaagaa tatttaaaga cttccaagct
    15421 tcaaaaatga atttaaacct ttacacaagc cccttgttag cctttgtttt tgtcttcata
    15481 ggagagtttg tggcttatac tttatatggt attagtttgt tagctctcat cggacttgct
    15541 agaaattttg gagaggctgg tcaaaatctt gcaagctact tgcagacctt gcatcagagc
    15601 ttgacggata aaacaagtga ctttcgttta attttaggat tactggcctt tggttttatt
    15661 cttaacactg tgttcagatg gacaagaaaa gttgagaaaa gacctattcg aaccttggga
    15721 ttttatagag agaattgcct cagcaatctt ctgaaaggat ttagtctagg cctggcactt
    15781 tttcttctga ccttgttaag tttagtggtc ttaggtcaat atcgtttgga atccattcac
    15841 ttgaatcctt attatcttgc ctttgtcgtc tttactatcc cattttggat tttacagggg
    15901 acagcagaag aagtggtggc ccgtgcttgg ctacttcctc aattggcctc aagaaccaat
    15961 ctaaaactag ctattcttat atctagcctg ttctttaccc tgcttcatat gggcaattct
    16021 ggcctcaccc ctctatctct agtaaatctc tttttattcg gagttgccat ggctctttac
    16081 cttctcaaaa ctgatacagt ttggggtgtt gcaggtattc atggtgcttg gaattttgct
    16141 cagggtaatc tctttgggat tttagttagt ggtcaaccgt caggaacgtc tctgatgacc
    16201 tttttaccac aaggcaatca agattggcta tcaggtggtt cttttggcat agaaggttcc
    16261 attatgacaa gtctggtatt actactgctg attgtctatc ttgctaataa attaaagaaa
    16321 gaaaatgaaa ggatgtgact tcggtccgtc cttttcttcg tgaaaatact ataagtatgc
    16381 taaaatagga atagcacatg gagagaggat tcttatgatc aatcacatta cagataatca
    16441 atttaaacta gtatcaaaat atcaaccatc aggagatcaa ccccaagcta tcgagcagtt
    16501 ggtggataac attgaggggg gagaaaaagc tcagattctg atgggggcga ctggaacagg
    16561 gaagacctat actatgagtc aggtcatttc taaagtcaat aaaccaactc tggttattgc
    16621 ccacaataaa actctggctg gtcagctcta tggggagttt aaggaatttt tccctgaaaa
    16681 tgcagttgag tatttcgtat cctactatga ttattaccag ccagaggcct atgtcccttc
    16741 tagcgatacc tatattgaga aggatagttc tgtcaatgac gagattgaca agcttcgcca
    16801 ctcagctacc tcagcccttt tggagcgtaa tgatgttatt gtcgtggcct cagtctcttg
    16861 tatctatggt ttgggttcgc ccaaggaata cgctgatagt gtcgttagtc tccgtcctgg
    16921 tctagagatt tctcgtgata aactcttgaa tgacttggtc gatattcagt ttgaacgcaa
    16981 tgatattgat ttccaacgcg gaagatttcg cgttcgtggg gatgtggtag agattttccc
    17041 agcttcccga gatgaacatg cctttcgagt agaatttttt ggagacgaaa ttgaccgtat
    17101 tcgtgaagtt gaggctctga caggtcaggt gttgggagaa gtggatcatt tagcgatttt
    17161 cccagcgaca cactttgtga ccaatgacga ccacatggaa gttgccattg caaagattca
    17221 ggccgagttg gaagaacaat tagctgtctt tgaaaaggaa ggtaaactgc ttgaagccca
    17281 gcgtttgaaa cagcggacag agtatgatat cgaaatgttg cgtgagatgg gctataccaa
    17341 tggggttgaa aattattctc gccacatgga tggacggagc gaaggagagc ctccttatac
    17401 gcttctcgac ttcttcccag atgatttctt gattatgatt gacgagagtc atatgaccat
    17461 agggcaaatc aagggcatgt acaatggaga ccgttcgcgt aaagaaatgc tggttaatta
    17521 tggtttccgt ttgccgtctg ctttggacaa tcgtcctctc cgtcgggagg agtttgagag
    17581 tcacgttcat cagattgttt acgtttcagc gacacctggt gactatgaaa atgaacagac
    17641 cgagacagtg attgagcaaa tcattcgtcc aacgggactc ttggatccag aggtggaagt
    17701 ccgtccgact atgggacaga ttgatgacct cttgggtgaa atcaatgccc gcgttgaaaa
    17761 aaatgagcgt acctttatca caactttgac caagaaaatg gcagaggact tgaccgacta
    17821 cttcaaggaa atgggtatca aggttaagta catgcactcg gatatcaaga ccttggaacg
    17881 gacggagatt atccgtgacc tgcgcttggg tgtctttgat gtcttggtcg gaattaacct
    17941 gctccgtgaa ggaattgacg ttcctgaagt gagcctcgta gctattctcg atgctgacaa
    18001 ggaaggtttc cttcgcaacg aacgtggact catccagact attggacgtg ctgctcgtaa
    18061 cagtgaaggc catgtcatca tgtatgcaga cacggttacc cagtctatgc aacgtgctat
    18121 cgatgaaact gcccgccgtc ggaaaattca gatggcctat aatgaagagc atggtatcgt
    18181 accacagaca atcaagaaag aaatccgtga cctgattgct gtgaccaagg cagttgctaa
    18241 ggaagaggac aaggaagtcg acatcaatag cctcaacaaa caagagcgca aagaactcgt
    18301 caagaaacta gaaaaacaaa tgcaagaagc cgtcgaagta cttgactttg aactggcggc
    18361 tcagatccgt gatatgatgt tggaagtcaa ggccttggat taggggaata gtatgattta
    18421 tttaagaaag ttaaagagag aagatttgat gtctttatgg gaaatggctt attcacaact
    18481 taatccagtt tggaaacagt atgatgctcc ctattatgat gattatcagt atttttcaaa
    18541 ttttaaagaa ttcgaactac aaaaatcaga atccatttta agcaactcaa atcgccttgg
    18601 tatttttgtt gatgataaac tagttgggac tgtttcgcgt tattgggtat gtaaacaaac
    18661 aagatggatg gaattgggaa ttggtattta tgataaaaaa ttctggaaca ctggtattgg
    18721 gaaagttgct atgttgcagt ggatagatag gacgtttcag gattacttgg agttggagca
    18781 tctgggtttg acaacttggt cagggaatct tggtatgatg aaacttgctg aaaaattaag
    18841 aatgaaaaaa gaagctcata ttccaaaagt tcgttattat caaggtaaat attttgatag
    18901 tattaaatat ggtattttga gagaagactg ggagaaaaca aatgacggtt attatcaaat
    18961 caatggaaac tcctgaagag atagaaggta agccccttgt tcactggcaa acgtggagag
    19021 aggcttatga tggccttttg cctgcagaat ttcaggagac aatgacatta gaaagatgtc
    19081 gattctttag tcaaaagtat ccagaaaata cattgattgc gatggatggt atgaaggtag
    19141 ttggttttat cagttatggc aactttcgtg atgagactat tcaagctggc gaaattattg
    19201 ctttatatgt tttaaaagac tattatggaa aaggtatcgc acaaaagtta ataaaagcag
    19261 ctttgattgc tcttaatcat ttttctgaaa ttttcttatg ggtattgaaa gataataagc
    19321 gcgcaattgc tttctatcaa aaaatgggct ttacttttga tgaacaagaa aagatacttg
    19381 aacttggaaa gcccataaag gaaaaacgga tggtattcta ttctaaataa ttttaaaaag
    19441 tagaagctaa tggtactggt atcaagtcta aaaatttaat aaattagaaa gtgagtaaat
    19501 ttatgtctcg agcacaatta acgattttaa caaatatttg tctgattgaa gatctcgaag
    19561 ctcagcgcgt ggtgatgcag tatcgcgctc ctgaaactaa tcgctggtct ggttatgcct
    19621 ttccaggagg ccatgtagaa aatggcgaat ctttcgcaga gtctgtcatt cgtgaaatct
    19681 atgaagaaac agggttgact atccaaaatc ctcaacttgt cggcattaaa aattggccac
    19741 tagatacagg tgggcgctat attgtcgttt gttataaggc aactgagttc tctggtaccc
    19801 ttcgctcttc agatgaagga gaagtttctt gggtgcaaaa agaccagatt ccaaacttgg
    19861 atctggccta tgatatgcta cctttgatgg agatgatgga agcatcggac aaatctgaat
    19921 ttttctaccc tcgccgtaca gaagacgatt gggaaaagaa aattttctaa tcctttacta
    19981 aataacctag ctgatccaag gcctcctcga tatagtggag gtcttgttgt gtttcggctt
    20041 cgactaggtg gtaatggaca ccatctgtca attcagataa aggtttaaaa tcagaatgct
    20101 cgacttgttc tagaaaatgt tgaacgtctc ggcggcaaga cagtttaagc aaggtttcaa
    20161 tttctccata aacgggatgg tcaatcaaag tattctggac atgcccacca ttatcgacaa
    20221 tagcaaggag ttcctgaccg atttcttcaa cttcatgttt cactttgaaa agtttgtgaa
    20281 cataaggatt ggtatcaatt tgcttataga tgtagccacg attggtggat aggataggag
    20341 cgccatcggc tctcaaaatt gcaatgtcct gcacaatgac ctgacgtgtg acatgaaaat
    20401 gttccgccaa actttggcca ttaagggctt taggagcttc tttcaagagt tggagcaggg
    20461 cttgtttgcg atcttttgtc atagcttttc cttttaacgg cgttttcgaa gcactttata
    20521 gacagctagt gctaatgtat agtctaccat actatggata attgtgccaa atccaactag
    20581 tacaaataga acatagaaca tgttttctac attggtaccc gaagttgcgt aaaaaaggac
    20641 acaggctaat acttcagcaa gggcatgaac aacagccaaa acaaaattga aaacccagga
    20701 agattttggt ttatctaggg tatcggggaa tttttgtagg taaagagatc ctaaagcacc
    20761 aaaagatata tgggaaaaag cccgaaaaac tataaccatg ggatagccag ccatcaaaaa
    20821 tccaaaacta gaggctagga tgacaaaaac tgccatcaag ggcgacaaga acatggctat
    20881 aaaaatagcg atgtggctcc ccaaagtata ggaagcaggt ggaatgacaa tcttgaaagg
    20941 cataacaatt ggaatcaaaa tcgcaatagc cgttaaaagg gctgtcattg tcataaattg
    21001 tgtctttttc cgtgtattca caagaatctc ctttttaact gcatatacac tagtatggta
    21061 caataaacca gacaataaag caagaattta cttgggttta tagatcattt tttagttaaa
    21121 agttatagta gatggaaact agaatagtcc acctctactt ctaaaacatt gttagcgatt
    21181 tgactgtcct gatcgatttg tcctgttctt atttcgtttt actatagtaa agatttcatt
    21241 aaaaagaaac tgtatagagc aaaatctcca ccttcaggtt tgaaagcgga gattgttttt
    21301 attttttcaa ggtttgtagt cgtggaacaa tggctagggt tagaattgca gaaatgacca
    21361 attcagcaat tgaatttgtt gagataacgg ttgccagaag aagttggata tttccattat
    21421 aaacatttcc aaataggaag aagattcctc caaggacaaa gatagtattt gtcaaggaac
    21481 caagggctcc agctaaaatc agaccagtct tgtttttcat cagtttatag actaagtaag
    21541 gagttaaacc aatcaaaata cgtgggacga tggcaatgat agctgagtag atgtttccgt
    21601 ttggtacgaa gggagagaag aggtagcttg tcggtagaat cgtaatcgtg ttaaccgtca
    21661 agctaagtaa tcccatcaaa aatccaagtg taaccccaac tcgtggacca taaataatgc
    21721 tggcaataat gacaggaata tgaacaatgg tcggtttgat tggaaatgga aaaaggttaa
    21781 agataagtga gctcagaaag tgtatcacga gcatggtagc aaaaaagata gcaatgggtg
    21841 caatattaga gcgttttttc atcgatagtt tcctttattc tttctaaaat aattgtgagg
    21901 tcagctaaag ctcctcgtcc gtggtctcca caagctagta gggattcctt aggagcaatc
    21961 agctgatagc cgtaggtttc taatgttttc agattattct gagttactgg atggtcatac
    22021 atttttgtat tcatagcagg agctattagt ttgggaatat gacttggtag ggctagagct
    22081 gtactggtta ccatgttgtc cgcaaatccg tgagctagtt ttgcaatagt gttagcagtt
    22141 gcaggtacca cgataaataa atctgctttt tttccaagtt cgatatgatt gacctgatca
    22201 ggatagggtt ccttcatgac atccaagtgg acaggattct gtgagagtac ctgtagtgtc
    22261 aaaggttgga taaactctgt agcagcctga gtcattaaga cagtgacttg atggccttgt
    22321 ttttttagag aactgactaa atctgccgac ttataagagg cgattgaacc cgttacagcc
    22381 aagagaatgt ttgccatagt tttcctttct aagaatgata ggcttgaatt ttttcaagga
    22441 ggagttctgc aatttcttct ttagtctgga ctgtttgaag ctgatttttc tcaacaaata
    22501 tagctcggtg ctgatctgct gaaatttgag tcaggtcatt cgcgatgatt aaatctgctt
    22561 gattcttgat aagacttttt cgtgcaatgt caaccagatg atcttcggta acatcaacca
    22621 gcagtttgaa accaatcaga tgaatagtgg gattccattc cttgactagg gatatgattt
    22681 tgggtgtctt tttaaggaac aaaacctgaa cctcatcagt tgaagaaatc ttggcctgat
    22741 gattctgctt gcttaaaaat tcttttagat tggagctagc ctgaacttcc tcaagccctg
    22801 tcatataaac aggagtgtag tcagaaacag ccattgagtg gatcaagacc tgataatcct
    22861 gaacacgttc ttgcatttct attagaaggt ccttggtatt ggtaatttct cgaatactta
    22921 ggttaggatg aggctctggc ttcagagctc gttttgtcgt aattaaacaa acttcatgcc
    22981 ctgcagaaag caaagtctct gtgataattt tccccaagtg acctgtagaa tggttagtga
    23041 tagagcggac gctatcgata gcttcactgg taccgcccga tgtaactaaa attttcatag
    23101 cactattgta cacaaaaata agcggtaagt aaaatgaaag tgataaattt ttggtaaaaa
    23161 cgaagattta ctatagtttc aaactataaa gccatataaa atttaagaaa ggactctaaa
    23221 agccttaaaa acagagatat ttacgaacgt ttagagagtt ttaggatgtt aaaaatcttg
    23281 ttttgtgata taatgaatta tcatataaga ggttagagga attttgaatg aaaacagata
    23341 ttgaaatcgc acagagtatt gagttgaagc caattgttga tgttgtagag aaacttggta
    23401 tttcttatga cgatttggag ttgtacggaa agtacaaggc taaactcagc tttgataaaa
    23461 ttcgtgcagt tgagagcaat ccagtcggta aattgatctt ggttactgcc atcaacccaa
    23521 cacctgcagg tgaaggaaag tcgacgctta ccattggtct tgcggatgcc ttgaacaaga
    23581 ttggcaagaa aaccatgatt gctatccgcg aaccgtctct tggtccagtc atggggatca
    23641 agggtggtgc tgctggtggt gggtatgcac aagttctgcc aatggaagac atcaacctcc
    23701 actttactgg agatatgcat gctattacaa ctgccaacaa tgccctttct gccttgattg
    23761 acaaccactt gcaccaaggg aatgagctgg aaattgatca acgtcgtatc ctctggaaac
    23821 gcgttgtgga cttgaacgac cgtgcgctcc gccatgtgac tgttggtctt ggtggtcctc
    23881 taaacggtat tccacgtgag gatggttttg atattacagt tgcttcagaa atcatggcaa
    23941 ttctttgctt ggcaacggac atcgaggact tgaaacgtcg tttggcgaat atcgttattg
    24001 gttatcgcta tgaccgtacg cctgtttctg taggtgattt gcaggttgag ggtgccttgg
    24061 ctttgatttt gaaggatgct attaagccaa acttggttca gacaatttac ggtacacctg
    24121 cctttgtaca cggtggtcca tttgccaata tcgctcatgg ctgtaactct gttttggcga
    24181 cgacaacagc ccttcacttg gctgattaca ctgttactga agctggtttt ggtgcggacc
    24241 ttggtgctga gaaattcctt gatatcaaga caccaaactt gccaacatct ccagatgcag
    24301 ttgtcattgt cgcaaccctt cgtgccctta agatgaatgg tggtgtggct aaagacgctc
    24361 tgactgaaga aaatgtagag gcagttcgtg caggttttgc taacttgaaa cgccacgttg
    24421 aaaatatccg taagttcggt attccagcag ttgtagctat taacgaattt gtatctgata
    24481 cagaagctga aattgcagtc ttaaaagaac tctgtgcctc aatcgatgta ccagttgaat
    24541 tggctagtgt ctgggctgat ggagcagaag gtggagtagc acttgccgaa acagttgtta
    24601 agacaattgc tgaaaatcca gctaactata aacgtttgta cgataatgcc ctttctgtcc
    24661 aagaaaagat tgaaaaaatt gtcactgaaa tctatcgtgg tagcaaagtg aactttgaga
    24721 agaaagctca aacacaaatt gctcaaatcg ttcaaaacgg ttgggacaaa ttgccaatct
    24781 gtatggctaa aactcaatac agtttctcag acaatccaaa tgcgcttgga gcaccagaaa
    24841 actttgaaat taccattcgt gaattggtac caaaattagg tgcaggcttc atcgttgcct
    24901 taactggtga tgttatgacc atgccaggtc ttccaaaacg tccagcagct ctcaacatgg
    24961 atgttgaaag cgatggaact gtactaggct tgttctagtg gattgaaatt gactttaagt
    25021 gagtttggaa ataatatcca agctcatttt ttctcagact gcaagcagtt catcaataag
    25081 atttatactc ttcgaaaatc tcttcaaact acgtcagctt caccttgccg taggtatatg
    25141 atactgactc tgtcagttct atccacaacc tcaaaacagt gttttgagca acctgcggct
    25201 agcttcctag tttgctcttt gattttcatt gagtataagt agatagaaaa gttatatact
    25261 atctattgaa aatattataa atcagataca aagacaggtg aaaccttctg tgttatacta
    25321 gagatatgtt agatttgaaa gaatacggta tcgtcatgtg gccggaggag aaagtcattt
    25381 ctttccgtga gaaacttctc gcttggtatg atgaaaacaa aagagatttg ccttggagga
    25441 gaagtaaaaa tccttatcac atctgggtat ctgaaatcat gcttcagcag accagggtgg
    25501 atacagttat cccttactac gaaagattcc tggactggtt tccaactgtc gaaagtctgg
    25561 caactgcgcc tgaggagagt ttactgaaag cttgggaggg cttgggctat tattctcgag
    25621 ttcgcaatat gcaggctgca gcccagcaga ttatgactga ctttggtggc caatttccaa
    25681 atacctatga aggaatttcc agcttgaaag ggattggacc ttacacagca ggagccattt
    25741 ccagtattgc ttttaacttg cctgagccag ctgtagatgg taatgtcatg cgggtcttgg
    25801 cgcgtctgtt tgaagtcaac cacgatattg ggattccaag taatcgaaaa atttttcagg
    25861 caatgatgga gatcttgatt aatccggatc gtccgggtga ttttaatcaa gccttgatgg
    25921 acttagggtc tgatattgag tctcctgtaa atcccagacc tgaagaaagc ccagtcaagg
    25981 actttagtgc ggcatatcag aatggaacca tggaccgtta tccaattaaa tctcctaaga
    26041 aaaagccggt tcctatttat cttaaagcat tagttgtgaa aaattctcaa ggacaatttt
    26101 tacttgaaaa aaatgaaagc gaaaagctct tggcaggttt ttggcatttc ccccttatag
    26161 aagttgataa cttttcgcaa gaagagcagt ttgacctctt tcatcaggtt gcggaagaaa
    26221 gtgtgaactt tggccccagt ccagaagaga gtttccagca ggactatgac ctagatgttg
    26281 attggcttga tgtttgtttt gatacagtcc agcatgtttt tagtcatcga aaatggcatg
    26341 tacagattgt agcaggtcag gtgagtgact tccatgattt ttcagatagg gaagttcgtt
    26401 ggctttcacc agaagaattc aagaattacc cacttgccaa accccaacaa aaaatctggc
    26461 aggcttatgc acaagccaac ttagacagta gccaagacta gctattgtgt cttctgacta
    26521 ttttttggta taatagtaga gaaaaaggtg aacatatgaa aaaaatacta attgtagatg
    26581 atgagaaacc aatctcggat attatcaagt ttaatatgac caaggaaggt tacgaagttg
    26641 taactgcttt taatggtcgt gaagcgctag agcaatttga agcagagcaa ccagatatta
    26701 ttattctgga tttgatgctt ccagaaattg atggtttaga agttgctaag accattcgta
    26761 agacaagcag tgtgcccatt cttatgcttt cagccaaaga tagtgaattt gataaggtta
    26821 tcggtttgga acttggggca gatgactatg taacgaaacc cttctccaat cgtgagttgc
    26881 aggcgcgtgt taaagctctt ctgcgtcgtt ctcaacctat gccagtagat ggtcaggaag
    26941 cagatagtaa acctcaacct atccaaattg gggatttaga aattgttcca gacgcctacg
    27001 tggctaaaaa atatggcgaa gaactagact taacccatcg tgaatttgag cttttgtatc
    27061 atttagcatc gcatacaggt caagtcatca cgcgcgaaca cttgcttgag actgtctggg
    27121 gttatgacta ttttggtgat gtccgtacag ttgatgtgac tgtacgacgt ctgcgtgaga
    27181 agattgaaga tacgcccagc cgaccagagt atatcttgac gcgccgtggt gtagggtatt
    27241 acatgagaaa taatgcttga tttactgaaa caaaccattt ttaccagaga ttttatcttt
    27301 atcctgattt tgttaggttt catccttgtt gtgaccctct tattactgga aaatagacgt
    27361 gataatattc agttgaagca aatcaatcaa aaggttaaag atttgattgc aggagattat
    27421 tccaaggttc ttgatatgca aggtgggtct gaaatcacca atattaccaa taatttgaat
    27481 gacttgtcgg aggttattcg tctcactcag gaaaatctag aacaagagag taagaggcta
    27541 aatagtattc tgttttatat gacagatggg gttcttgcga ccaaccgtcg gggtcagatt
    27601 atcatgatta acgatacagc caagaagcaa ctggggttgg ttaaggaaga tgttctgaat
    27661 agaagcattt tggaattgct caagatagaa gaaaactatg aattgcgtga tttgattacc
    27721 caaagtccag aattgttgct agattcccaa gatatcaatg gcgaatattt gaaccttcga
    27781 gttcgctttg ccttgatacg tcgagagtct ggctttattt caggtttggt ggctgttttg
    27841 catgatacga cggagcagga gaaggaagaa cgcgaacgaa gactctttgt ttccaatgtt
    27901 agccatgagt tacggactcc tctgactagc gtaaaatcct atcttgaagc cttggatgag
    27961 ggggctttgt gtgaaactgt agcaccagac tttatcaagg tttctcttga tgagaccaac
    28021 cgtatgatgc gcatggtgac ggatctcctc catctttcac gtattgataa tgctaccagt
    28081 cacctagatg tggaactgat taacttcact gcttttatta cctttatcct caatcgtttt
    28141 gacaagatga aaggacagga aaaggagaaa aaatatgagt tggtgagaga ttatcccatc
    28201 aattctatct ggatggaaat tgatacagat aagatgacgc aggttgtcga caatatttta
    28261 aataatgcta ttaagtattc gccagatggg ggtaaaatca ctgtcagaat gaagacaact
    28321 gaagaccaga tgattttatc catttctgac cacggtttgg ggattcctaa gcaggattta
    28381 ccacgtatct ttgaccgttt ctatcgtgtg gatcgtgcta gaagtcgtgc acaaggtggt
    28441 acaggtctag gactgtctat cgctaaagaa attatcaaac aacataaggg ctttatttgg
    28501 gccaagagtg aatacggcaa gggttcaacc tttaccattg tgctccctta tgataaggat
    28561 gcagtgaaag aagaagtatg ggaggatgaa gtagaagact agaatgagtg aaataggctt
    28621 taaatacagt attttagcgt cgggttccag tggaaattct ttttatctgg aaacctcaaa
    28681 aaagaagctt ttagtagatg caggcttgtc tggcaagaaa attaccagtc tgctagctga
    28741 aattaaccgt aagccagaag acctggatgc catcttgatt acccatgagc attcagatca
    28801 tatccatgga gtaggcgttt tggctcgcaa gtatggtatg gatctttatg ccaatgaaaa
    28861 gacctggcaa gctatggaaa atagtaaata tcttggcaag gtggattctt cgcaaaagca
    28921 tatttttgaa atgggtaaaa ccaaaacctt tggagatatc gacatcgaga gttttggtgt
    28981 aagccatgat gcagtcgcac cgcagttcta tcgctttatg aaggatgata agagttttgt
    29041 cctcttgaca gatacaggtt atgtcagtga ccgtatggcg ggcattgtcg aaaatgcgga
    29101 tggttatctt atcgaggcca accatgatgt agagattttg cgatcaggtt cttacgcttg
    29161 gcgcctcaaa caacgaatcc tatctgacct tggtcacctt tctaacgagg acggtgctga
    29221 agctatgatt cggacgctag gaaatcgtac taagaagatt taccttgggc atttatctaa
    29281 ggaaaacaat atcaaggaac tggctcatat gaccatggtc aatcagctgg ctcaagctga
    29341 tctgggagtc ggagtagact ttaaggttta tgatacctca ccagataccg caacaccatt
    29401 gacagagata tagaaagaac gctgagaagg tgttcttttt atattgactg gacacctaaa
    29461 aagtaataca atggtgttac cattaaaaaa gggagcacaa aagatgacta ctattacatt
    29521 aaaagtttct gaagctgata aaacatttat gaaagcaatg gctaagtttg aaggagtttc
    29581 cctttcggaa cttattcgca ccaaaactct tgaagcccta gaagatgaat acgatgctcg
    29641 tgtggcagat ttagcctatc aagagtattt agaagacttg gaaaaaggag ttgaacccat
    29701 tacttgggaa gaaatgatgc atgatttagg cttgaaggat gaataatttg tataaattag
    29761 ttccaacaag acgttttatc aagcaattga aaaaattgga ccgttatacg cagaagctaa
    29821 ttacaaacta tttacaaacc aatgttttgg aagatccaag acgacacgga aaggctttgg
    29881 ttggtaatcg cgttggtcaa tggcgctata gaattggtaa ttatcgagtt atcgtacaaa
    29941 ttgtagatga tgaattagtt gttgctactc tagaagttgg tcatcggaga gatatttatt
    30001 aaattaaagc tgagtaataa cagtatagaa agtaaggaga gtaaaaattt gaagaaaaaa
    30061 tggttttttg cagattatta tgacacaaca attattttac ttgctttaat ctctgttatt
    30121 ctggtattac ttggatttgc tgagatgatt gatttggaca atccgcctta tagtattatt
    30181 gatttagtaa tctggggtgt ttttgtaatt gattatagtt ggcgcttttt tacgactaaa
    30241 agaaaatggc gctttattct tgaaaatatt tttgatttat tggctattct ccctttaaat
    30301 gctattttta cagtgtttcg attagggcgt atctttcgtt tagctaaact aacaaaatta
    30361 ctgaaactga ctcgtttact tagaataatt ggacttacag gtaaattaga aagaaaaatt
    30421 agtagattct tacggacaaa tggcctgatt tatatcttgt atgttaatat ctttattgtt
    30481 ctagtaggga gttcgatttt atccgttgtt gaagaaaaat ccttctcaga tagtctttgg
    30541 tgggctcttg tgacagtaac tactgttgga tatggggata ttgttcctgc ttccattttt
    30601 gggaaatggc tagcagtttt actgatgctt gtcggtattg gaacaatagg gatgttaaca
    30661 agtgctttga cgaacttttt tgtaaaggat aatccagatg aacagataaa acttgataaa
    30721 ctcaaagatg aattatcgag ccaaagaata ttactagaga aacaatctaa aaagatagaa
    30781 gaactacata aaatgataca agatttgatt gaaaaaacat aataaaaata aagatgagac
    30841 aaagtttgag ctgaggagaa atcaagtaag ttttgacaca atagaacgca tattatcagg
    30901 tataaaaact tgatactatg cgttttatag agaaataaaa taaaatctga acaaatcaat
    30961 taggaattta aagcatttta taacaacgtt ttagagtgat ggggtgctat ttcaacttca
    31021 acctactata atacagaaaa aaacaactcc ctgatgattc aaggagttgt ctatagttaa
    31081 attagttttt agaagcttct tggaattctg gatttttcca tgcttcgtca ataatagctt
    31141 gcaattcttt agcagatgct tgcatttttt gagtttctgc atcgttcaat ggaatgttta
    31201 ctggacgaac gataccatgt gcaccaacaa cagctggttg accgataaag acattctcaa
    31261 ctccgtatag accttcttgg aatactgaaa gtggaagtac tgcgttttca tcgtcaagga
    31321 ttgctttagt gatacgagca agggctactg cgataccgta gtatgttgca ccttttttgt
    31381 tgatgattgt gtaggctgca tcacgaacac cttcgaacaa ttcaatcaat tcagcttctt
    31441 gaacattttg agtgtcttta aggaattctt caaggtttac accagcgatg ttagcgtgtg
    31501 accaaacagc gaactcagag tcaccgtgtt cacccatgat gtaggcgtgc actgaacgag
    31561 catccacatc caatttttca gcaagtgctt gacggaaacg agctgagtca agtgaagtac
    31621 ctgaaccgat aacgcgttct ttagggaaac cagagaattt ccaagttgag taagtcaaaa
    31681 cgtcaactgg gttagcagca acaaggaaga tacctttgaa accagattca acaacttgag
    31741 ttacgattga tttgttgata gcaaggtttt tacctacaag gtcaagacga gtttcacctg
    31801 gtttttgagg tgcacctgca gtaatcacaa caaggtcagc gtctgcacag tcagagtatt
    31861 gagctgcata gattttttta ggtgaagtga aggcaagggc gtgactaagg tcaagcgcat
    31921 caccaacagc tttttcatgc aattgtggaa tttcgataat tccaagctct tgtgcaattc
    31981 cttggttaac aagtgcaaaa gcgtaagatg aacctacagc accatcaccg acaaggataa
    32041 cttttttgtg ttgtttagtt gaagtcattg ttttaaacat ctccttaatt ttattagggg
    32101 attttcccta gacaacttca ttctatcact tttaaaaaac tttgtcacga atatgcctta
    32161 tagttctcga tgtaaacgtt ttagtggttt agaggctgaa atagatggga atttatggta
    32221 taatgttgtt acttactaat tgtgaaatga ggcatttatt aatgcaggat aaaaatttag
    32281 tgaatgtcaa tctgacaaag gagatgaagg caagttttat cgactacgcc atgagtgtta
    32341 tcgtagcgcg agctcttcct gatgttcgag atggcttaaa acctgttcac cgtcgcattc
    32401 tctacggaat gaatgaattg ggtgtgaccc cagacaaacc ccataaaaaa tctgctcgta
    32461 ttacagggga tgtcatgggt aaatatcacc cacacgggga ttcctctatt tatgaagcca
    32521 tggtccgtat ggctcaatgg tggagctacc gttacatgct tgtagatggt catgggaatt
    32581 ttggttccat ggatggagat agtgctgccg ctcaacgtta taccgaggca cgtatgagca
    32641 agattgctct ggaaatgctt cgtgatatca acaaaaatac agttgatttc gttgataact
    32701 atgatgccaa tgaacgggaa cccttggtct tgccagcgcg ttttccaaac cttttggtta
    32761 atggagcaac tggtatcgcg gttgggatgg caaccaatat tccacctcat aatctgggtg
    32821 aaaccattga tgcagtgaag ttggtcatgg ataatcctga agtgactacc aaggacttga
    32881 tggaagtctt gcctggacca gattttccaa ctggtgctct tgtcatgggg aaatcaggta
    32941 tccataaggc ttatgaaaca ggtaaaggtt cgattgccct acgttctcgt acagagattg
    33001 aaacgactaa gactggtcgt gagcgcatcg ttgtaacaga atttccttac atggtcaata
    33061 aaaccaaggt gcatgagcat attgttcgct tggttcagga aaaacgcatt gagggtatca
    33121 cagcagtacg tgatgagtca aaccgtgaag gtgttcgatt tgttattgaa gtcaagcgcg
    33181 acgcctcagc caatgttatt ctcaataacc tcttcaaaat gacccaaatg caaaccaatt
    33241 ttggtttcaa tatgctcgct atccaaaatg gtataccgaa aattttgtct cttcgtcaga
    33301 ttttggatgc ttatatcgag caccaaaaag aagtggttgt tcgtcgtaca cgttttgata
    33361 aggaaaaagc ggaagcgcgc gctcatatct tagaaggtct cttgattgcg ctagaccata
    33421 tcgacgaagt gattcgtatc atccgtgcta gtgaaacgga tgcggaagct caagctgagt
    33481 tgatgagcaa gtttaagctt tctgaacgtc aaagtcaagc tatccttgat atgcgtcttc
    33541 gtcgtttgac aggtttggaa cgcgataaga ttcaatctga gtatgatgac ctcttggctc
    33601 tgattgcgga tttagcagat attcttgcta agcctgaacg tgtttctcaa attatcaaag
    33661 acgaattgga tgaagttaaa cgtaaatttt ctgataagcg ccgtacagag ttgatgattg
    33721 gacaggtctt gagtctcgag gatgaggact tgattgaaga atcggatgtc ttgattaccc
    33781 tttctaacag aggctacatt aagcgtttgg atcaggacga gttcactgct caaaaacgtg
    33841 ggggtcgtgg tgtccaagga acgggagtga aagatgatga ctttgttcgt gagttagtgt
    33901 caactagcac ccatgatcat ctgctcttct tcacaaacaa gggacgtgtc tatcgtctta
    33961 aaggttatga aattcctgag tatggtcgga ctgccaaagg gctaccagta gtcaatctct
    34021 tgaaattgga tgaagacgaa agtattcaga cggttatcaa tgttgagtct gatcgcagtg
    34081 atgatgctta tctcttcttt acaacccgtc acggtattgt gaagagaacc agtgttaagg
    34141 agtttgccaa tattcgtcaa aatggtctca aagcgctgaa tttaaaggat gaagatgagt
    34201 taatcaatgt cttgttggca gaaggagata tggatattat cattggtacc aagtttggtt
    34261 atgcagttcg ctttaatcaa tcagccgttc gtggtatgag tcgtatcgcc actggtgtga
    34321 aaggtgttaa ccttcgtgaa ggagacacag ttgttggtgc cagcttgatt actgatcaag
    34381 atgaggttct tattatcaca gaaaaaggat atggtaagcg tacagtcgct actgaatacc
    34441 caacaaaagg tcgtggtggt aagggaatgc agacagctaa aattaccgaa aaaaatggct
    34501 tgctggccgg tcttatgact gttcaagggg atgaggattt gatgattatc actgatacag
    34561 gtgtcatgat tcgaaccaat cttgccaata tttcacaaac aggacgtgca actatgggag
    34621 ttaaagtaat gcgcctggat caagatgctc agatagtgac tttcacaacg gttgcggtgg
    34681 cagaaaaaga agaagttggg acagaaaacg aaacagaagg tgaagaataa tgtctcaaaa
    34741 aaataataaa aagaaaaaca agcgaaaaaa tctgctgaca aatatcctag caggatttct
    34801 gatattactg tcactggctt tgatttttaa tactcaaatt cgaaatattt tcatagtctg
    34861 gaataccaat aagtatcaag ttagccaggt atcaaaagaa aaattagaag aaaatcagga
    34921 tacagaaggc aattttgact ttgattctgt caaagctatc tcttcggaag ctgttctaac
    34981 ttctcaatgg gatgctcaaa aattaccagt tattggggga attgcaattc ctgaattgga
    35041 aatgaatttg ccgattttta aaggacttga taatgttaat ctcttctacg gagctggtac
    35101 aatgaaacgc gagcaagtaa tgggagaagg aaattatagt ctagctagtc accatatctt
    35161 tggtgttgat aatgctaata aaatgttatt ttctccttta gataatgcta aaaatggcat
    35221 gaagatttat ctaaccgata aaaataaagt ttatacttat gaaatacgtg aagtcaaacg
    35281 tgtgacaccg gatcgtgttg atgaagttga tgatagagat ggggtcaatg aaatcacatt
    35341 agtaacctgt gaagaccttg ctgctacaga acgtattatt gtcaaaggtg atttgaaaga
    35401 aacaaaagat tattcacaaa catctgatga aattctaaca gctttcaatc aaccatataa
    35461 acaattttat taatacaaat cagtgaaatc atggatttca ctgatttttt aagattttca
    35521 taaaaatata gtaaaatgaa ataagaatag gacgaatcgt tcaggacagt caaatcgatt
    35581 tctaacaatg ttttagaagt agaggtgtac tattctagtt tcaatctact ataaactttt
    35641 atgaaataca gaaaacgctt cctaaaacct aaagtttgtg atataattat caagaaaaaa
    35701 gatttaggag tttattatgg tttcttcaga atttatctca aagattgaat ttgcttgcaa
    35761 taagaaagaa agtctttata gtcaaagcaa atttaagtat gcgattcgtt cgatgttcgc
    35821 aggtgcattt ttaaccttca gtactgctgc aggtgcagtt ggggctgact tgattaataa
    35881 aattgcacca ggtagtggac gcttcctctt tccattcgtt tttgcttggg gcttggccta
    35941 cattgttttt ttgaatgccg agttggtcac ttcaaacatg atgttcttga ctgctggtag
    36001 tttcttaaaa aaaatctctt ggagaaaaac agctgagatt ttactatact gtaccttgtt
    36061 caaccttatc ggagccttga tagcagggtg gggctttgct cattcggcag cctatgcgaa
    36121 tctgacacac gatagtttca tctcaggtgt tgttgagatg aagttaggcc gctcaaatga
    36181 attggtcttg cttgaggcga ttttggcaaa tattttcgta aatattgcga ttctgtcatt
    36241 tattttggtc aaagatggtg gtgccaaact ttggcttgtg ttgtcagcta tttacatgtt
    36301 tgtattctta acaaacgagc acattgcggc gaactttgct tctttcgcga ttgtgaaatt
    36361 cagtgttgct gcggattcaa ttgccaactt cggtgttgga aatatgcttc gccactgggg
    36421 tgtgactttc atcggaaact ttatcggagg aggcctcttg atgggtcttc catatgcctt
    36481 cctcaataaa aacgaagata cttatgtaga ttaagaaaat gagcacgatt gagtcgtgct
    36541 tttttcattt tcaaaataag gtaatagcta tttcttatat caaaatatag aaaactgata
    36601 tttgtaaact ataactcaag gtgctacaat atccttaata aaatgatatg gaggtcacct
    36661 tatgacttgt gattttaaat ttgaaactct acaactacat gctggtcaag ttgtggctcc
    36721 agctactaag tctcgtgcag tgccgattta tcaaacaaca tcttttattt ttgatgacac
    36781 gtaggaaggt gctgatctgt ttgccttgag gaaaccaggg aacatttata ctcgtatcac
    36841 caatcctaca acagctgccc ttgaaggtgg tgttgaagcg ctagcaacag catcaggtat
    36901 gactgcagtg acttatacga ttttggcgct tgcccatgct ggtgaccatg tagtggctgc
    36961 ttcgactatt tacggtggaa ccttcaatct tttgaaagaa ccccttcctc gttatggtat
    37021 cacaacaacc tttgtcgatg ttgataattt ggaggaagta gaagcagcta tcaaagacaa
    37081 taccaagctt gtcttgattg aaaccttggg taactccttg attaatattc cagacctgga
    37141 aaaactggca gagattgctc ataaacatca aatcccactt gtgtcagaca atacttttgc
    37201 aataccttat ttgattaacg tcttctctca tggcgttgac attgccattc actctgcgac
    37261 taagtttatc ggtgggcatg gtacaactat tggaggaata attgtcgata gtggtcgttt
    37321 tgactggacg gcttcaggga aattccctca atttgttgac gagggtccaa gctgccacaa
    37381 tttgagttat acttgtgatg tgggtgcagc agcctttatt atagctgttc gagttcaatt
    37441 gcttcgtgat acaggtgcag ccttgtcacc attcaatgct ttcctcttgc tacaaagact
    37501 tgaaacctct tcacttcgtg tggaacgcca tgtacaaaat gctgagacaa ttgttgattt
    37561 tcttgtcaac catcctaagg tagaaaaggt aaattatcca aaacttgcag atagtcctta
    37621 tcatgccttg gctgagaaat acttgccaaa agatgtcggt tcaatcttta ccttccatgt
    37681 caaaggtggc gaggaagaag cacgcaaggt cattgataat ttagaaatct tttctgacct
    37741 tgcaaacgcg gcagatgcta aatcgcttgt tgtccatcca gcaacaacca ctcacggtca
    37801 attgtcagaa aaagacctag aagcagcagg tgtcacacca aactaaattc gtttgtcaat
    37861 cggtcttgaa aatgtagaag atttgattga agacttgcgc ttggccttgg aaaaaattta
    37921 aagtaaaaga agataaacag tgggcttcga ctcactgttt ttgattttcc ctcaggcatg
    37981 atataatggt taaagaagtc tagaaagagg aacgatatga acgaaatcaa atgtcccaac
    38041 tgtggggaag tctttacagt aaatgagagt cagtatgccg aactcttgtc ccaagtgaga
    38101 acggcagagt ttgataagga actacacgat aggatgaagc aggaactggc cttggctgag
    38161 caaaaggcca tgaatgagca acagactaaa ctggctcaga aggatcaaga aattgcgcaa
    38221 ttacagagtc agatccaaaa ctttgataca gaaaaagaat tggccaagaa agaggttgaa
    38281 cagacaagcc atgaggctct cttggctaag gacaaggaag tacagctctt agaaaatcag
    38341 ttggctacct tgcgtttgga gcatgaaaat caactgcaaa agaccctttc tgacctagaa
    38401 aaagaacggg atcaggttaa aaaccaacta cttttgcagg aaaaggaaaa tgaattatct
    38461 ttggcttctg ttaagcaaaa ctacgaagcc cagctcaagg cagctagtga acaagtcgag
    38521 ttttataagg attttaaggc tcaacaatct acaaaagcga ttggggaaag cctagaacag
    38581 tatgcagaga gtgagtttaa caaggttcgt agtttcgcct ttccaaatgc ttactttgag
    38641 aaggataaca aggtctcttc gcgtgggtct aaaggggact ttatcttccg tgagtgtgat
    38701 gaaaatggag ttgaaatcat ttctatcatg tttgagatga aaaacgaagc agacggaaca
    38761 gagaagaagc acaagaatgc agatttttac aaggaattgg acaaggaccg tcgggagaag
    38821 aactgtgagt atgccgtttt ggtgaccatg cttgaggccg ataatgacta ctttaacaca
    38881 gggattgttg acgtcagcca cgagtatgaa aaaatgtatg ttgttcgtcc tcaattcttt
    38941 atccagttga ttggtctctt acgtaatgcg gcgctaaatt ccctaaaata caagcaggag
    39001 ttggccttgg ttcgcgagca aaatattgac attacgcatt ttgaggaaga cttggatgcc
    39061 tttaagctag cttttgctaa gaactataat tcagcttcga ctaactttgg aaaagctatt
    39121 gatgaaatcg acaaggccat caaacgcatg gaagaggtta agaaattcct gactacatct
    39181 gaaaaccaac tccgtctagc taacaacaaa ttggaagatg tctctgttaa aaaattgacc
    39241 cggaaaaatc caacaatgaa agcgaagttc gaagcactga agggggagta gaaagcaaaa
    39301 atgaacggta ttatcaactt aaaaaaagaa gcaggaatga cctcgcatga tgcggttttt
    39361 aaactgcgta agattttggg aaccaagaaa attggtcatg gtggaacctt ggatccggat
    39421 gtggtgggtg ttttgccgat tgcggttggc aaggcgacac gcatggtcga gtttatgcag
    39481 gacgagggta agatctatga gggggaaatc attctgggct attccacgac gactgaggat
    39541 gctagtgggg aagtggtcgc agaaacccct gttttgtctc ccttggatga aaagcttgtt
    39601 gatgaagcga ttgctagctt gactgggcct attactcaga ttccccctat gtattcggca
    39661 gttaaggtta atggtcgcaa gctctatgag tatgcgcgtg ctggtcagga agtggagcgt
    39721 ccagaacgtc aggtgaccat ttatcaattt gagcgaacaa gtccgatttc ttatgatggc
    39781 caacttgccc gattcacttt tcgtgtaaaa tgcagtaaag ggacttacat ccgtactttg
    39841 tcagttgatt tgggtgaaaa gcttggttat gcggctcata tgtcccattt gactcgtact
    39901 agtgctgctg gcttacaatt agaagacgct cttaccttgg aggaaattgc tgaaaaagta
    39961 gaggctgggc aattagattt tctccatcct ttagagattg ggacaggtga ccttgtcaaa
    40021 gttttcctaa ctccagaaga ggctacagaa gttcgctttg gtcgttttat tgagctagac
    40081 caaacggaca aagaactggc tgcctttgaa gatgataaat tgttagccat tctagaaaaa
    40141 cggggcaatc tctataagcc aaggaaggtt tttagctaga tcgtttagga ataaaaatcg
    40201 ggtgatagat aacaattgct tgataaaccc ccatactaat agtagaatgg ttttgggaat
    40261 tataatattc caattgttgc gagttgtaga tactcaaata atctatatag aaatttagag
    40321 gtgtgaaatg aagcaactta aaattctttc agataaatat ttagagtcca tgacaggttc
    40381 tggtgggaac ttaggtctag gatttggtgt gataattcca tgatgtgaaa tgagtttcga
    40441 gaaagggtgg agcaacttct tcaacaaaaa gaaataaatg aaaatagtga gttgagtcac
    40501 ctgtttcgtc ttgctataca aaatttagac agaaatgaaa aataccaatc ggtcatggcc
    40561 aatttgagtc aagggttgtc actttacctc atgacgcatc attaccaggc acctaagtct
    40621 gtcattgatt ttggtttatg gattgccaaa gctcctagcc aggaaagagg gagactggct
    40681 ttcttgcaga tgcttgctca aaccctacag ggctttcgtt aaaactgtcg aacatcagag
    40741 ggaagactct ggtgtttttt gctgatgaga ctataaattt tgtaaaaaat gtgatagaat
    40801 agacgacgga taaaataacg gaggatagca tgcaaaatag accaatcatt attggagtga
    40861 caggtggttc tggtggtggt aaaaccagtg tttcaagagc cattttatcg cattttcctg
    40921 atgaaaagat ttccatgatt gagcatgatt catactacaa ggatcagtct catttgacct
    40981 ttgaagagcg tgtcaaaacc aactatgacc atccttttgc ctttgataca gacttgatga
    41041 tcgagcagat taaggaattg ttggcagggc gtccggtgga catcccgacc tacgactata
    41101 cagagcatac acggagtagc aagacctatc gtcaggagcc tcaagatgtc tttatcgttg
    41161 agggcatttt ggtcttggag gacaagcgtc tgcgcgattt gatggatatc aagatttttg
    41221 tggatacgga tgacgatgtg cgtattattc gtcgtatcaa gcgtgatatg gaggaacgtg
    41281 gccgtagcct tgatagcgtt attaaccagt acttaggtgt ggtcaaacca atgtaccacc
    41341 agtttatcga gtcaactaag cgttatgctg atatcgtcat tcctgaaggg gttagcaata
    41401 ccgtggctat cgacctgttg acgaccaaga ttgcaaagat tttggaagaa gctcgaaaca
    41461 gcaaataatc acatgtggag gccttgcctc ctttttctat ttttccctca taatggtaca
    41521 taaatggaga tttttaggca tatttttggt ataataatac ccataaaaga gcaagtaaat
    41581 gaatagtagg tggagatgga aaagtattta tcggtaacaa ctttgaccaa atatctgaaa
    41641 atgaaattcg ataaagaccc atacttggaa cgggtctatt taactggtca agtttccaac
    41701 tttcgtaaac gacctactca ccaatatttc tccctaaagg atgaccatgc agttattcaa
    41761 gcgaccatct ggtctgggat ttatcagaaa ttagggtttg acctggaaga aggaatgaag
    41821 atcaatgtga ttgggcgtgt acaggtctat gaaccaagtg gtagctactc catcatcatt
    41881 gaaaaagctg agcctgatgg ggttggggcg cttgcgattc agtttgaaca acttaagaaa
    41941 aaattgacag aagaaggcct gtttcaagaa cgcttcaagc aagctctacc ccaattttct
    42001 aagagaattg gggtagtaac cagccgtagt ggagccgtta ttcgagatat tatcacgacc
    42061 gtcagcaggc gattcccagg tgttgacatc cttctttatc ctaccaaggt tcaaggtgaa
    42121 ggggctgcgg aggaaattgc tagaaatatt gcgcgtgcta atcaacggga cgatttggat
    42181 ttgctcatta ttggtcgtgg aggtggttct attgaggatc tctgggcctt taacgaagaa
    42241 attgtggtac gagctatttt tgaatctcgt ttgccagtta tctctagtgt ggggcatgag
    42301 acggatgtga ccttggcaga ttttgtggca gatcgacgcg ctgcaacgcc aacagcggcg
    42361 gctgaactgg caacacctgt gaccaagttg gatgtattag ctcatttgca aaatcaggaa
    42421 aaacggatgg caacggcagt ccgaaatgtt ctatctaaga aacaagaggc tttgaaaaaa
    42481 tgcagtcagt ctgttatctt tagacaacct gagcgcttgt atgacggtta tttgcaacgc
    42541 ttggaccaac tgcaactgcg tttgaaacaa agtttgcgaa ctcggatttc tgataacaaa
    42601 caattagttc aagcaagaac tcatcaatta gtacaattat cacctgttac caaaatccaa
    42661 cgctatcaag accgtttagg acagttggac aagctcttag gtagccaaat ggcgttagtt
    42721 tatgacgcca aggttgctga ggccaagcga ctttcggaag ctttgctcat gttggatact
    42781 agccgaatcg tggcgcgtgg ttatgctatt gtcaaaaaag aagagtccgt tgtagattcg
    42841 gttgagagtt tgaagaaaaa agaccaagta acgcttttga tgcgagatgg tcaagtagaa
    42901 ttagaggtta aagatgtcaa aacaaaagaa atttgaggaa aatctagcag aactggaaac
    42961 cattgtccaa agtttggaaa atggtgaaat tgctctggaa gatgcgatta ctgcctttca
    43021 aaagggcatg gtcttgtcaa aagagctcca agctacgctg gacaaggctg aaaagacctt
    43081 ggtcaaggtc atgcaagaag acggaacaga aagtgatttt gaatgaaaaa gcaagaaaaa
    43141 ttagctcttg tcgagtcggc tttggaagat ttttatggag accagcagtt tgcctctagt
    43201 ttacgggagt ctgttctcta ttctattcat gctggtggca agcgtattcg gccttttctc
    43261 ttgttagaag ttctggaatc cttgcaggtt accatcaaac ctgctcacgc gcaggtagct
    43321 actgccttgg agatgattca tacagggagc ttgattcacg atgaccttcc tgctatggat
    43381 gatgacgatt atcgaagagg gcggttaacc aatcacaaga aattcggtga agctatggcc
    43441 attttggctg gagatgcctt attcttagac ccatatgcct tgattgcgca ggcagatttg
    43501 ccaagtcaga tcaaggtgga cttgattgcc aacttatccc ttgcttcagg tagtctgggt
    43561 atggtggcag ggcaagtttt ggatatggag ggcgaacacc agcacttgtc tctggaagaa
    43621 cttcagacta ttcatgccaa taagactggg aagttactag cctatccctt ccaagcggca
    43681 gctattatag ctgaattgtc acctgaaatg caggtgaagc tgaaaactgt gggtgaattg
    43741 attggacttg cttttcaagt cagagatgat gtactggatg tgacagctag ttttgaggaa
    43801 atcggcaaga cacctcaaaa ggatctgcag gcagaaaaat caacctatcc tgccttgttg
    43861 ggcttggaag agtccattgc cttttgtaac caaaccctgg atcaagctaa tgaaaaatta
    43921 gaagaaattg cccagcagct tccctttgaa acagaatcga ttgtaagtgt agtagaaagt
    43981 ttgagaatca atggctaagg aaagagtgga tgtactagct tataaacagg ggttgtttga
    44041 aacgagagag caggccaagc gaggtgtgat ggctggtcta gtcgtagcag tccttaatgg
    44101 agaacggttt gacaagccag gagagaaaat tccagatgac accgaattaa aactcaaggg
    44161 ggagaaactc aagtatgtca gccgtggtgg tttgaaactg gaaaaggcct tgcaggtctt
    44221 tgatttgtcg gtggatggcg cgactacgat tgatatcggg gcctctactg gaggttttac
    44281 cgatgtcatg ctacagaata gtgccaagtt ggtctttgca gtcgatgttg gtaccaatca
    44341 gttggcttgg aaattacgcc aagacccacg agttgtcagc atggagcagt tcaattttcg
    44401 ctatgctgaa aagactgatt tcgagcagga gccgagcttt gccagtattg atgtgagttt
    44461 catttccctt agtctgattt tgccagcctt gcacagtgtc ttggctgatc aaggtcaggt
    44521 ggtagcactt gtcaaacctc agtttgaggc aggacgtgag cagattggga aaaatggaat
    44581 tattcgagat gctaagattc atcagaatgt ccttgaatct gtaacagcta tggcagtaga
    44641 ggcaggtttt tcagtccttg gcttggactt ttctcccatc caaggtggac atggaaatat
    44701 tgaattttta gcgtatttga aaaaagaaaa gtcagcaagc aatcagattc ttgctgagat
    44761 taaagaagca gtagagaggg cgcatagtca atttaaaaat gaataaaaaa gaaagacttg
    44821 aaaaaattag aagattggtg acagattatc aaatcggcac gcaagaagaa attgtagaac
    44881 atttgaaaga agcaggtatc actgccactc aggcgacggt atcccgagat atcaaagagt
    44941 taggtattgt caaaattcct ttgagagaca acacctatgt ctacgaattg ccaaaatcaa
    45001 tcgtaaaaag tctgcaactg gctgaagaca atatcgaatc ggctgaattg atggataaga
    45061 tgatcaatct ccaagttatt ccaggaaata cggcttttgt aaaagctcag ttaatcgaga
    45121 cttttgcaga caagattttt agctgtttga ctgatgatag ctcgatttta gtcattgcca
    45181 gaagtggaag tctagcagag gaaatctttg aacaagtaaa aaattggtag gtctatatgt
    45241 tacttgaaat ttcgataaaa aactttgcca ttattgaggc tatttccctc aattttgaaa
    45301 aggggatgac tgtcctgact ggtgaaacgg gtgcagggaa gtcgattatc attgatgcca
    45361 tgaatatgat gttgggagct cgtgcgacga cagatgttat tcgtcatggt gcaccaaagg
    45421 cagagattga ggggcttttc tcagttgaga atagtcgtct tttacaggaa atttttgatg
    45481 agcaaggttt agaattaggt gatgaaatta tcatccgtcg ggaaatcttg caaaatggtc
    45541 gtagtatcag ccgtgtaaat ggtcagatgg ttaatctgtc tgttttgcga actattgggc
    45601 aacatcttgt agatattcat ggtcagcatg accatgagga gttaatgcgt ccccaactgc
    45661 atatccagat gttggatgaa tttggtgatg ccgctttttg ggacttgaaa gaaacctatc
    45721 aaacgagttt tgatgcctat cggaaaatgc gcaagcaggt tctggaagtc aagaaaaacc
    45781 aacaggaaca caaggcacgt atcgaaatgt tggaatttca aatggcagag attgaggcag
    45841 caaacttgca ggctggagaa gacttggatc tcaatcaaga gcgagataaa ctcctcaacc
    45901 acaaaaatat tgcggataca ctgaccaatg cctacagtat gttggacaat gaagattttt
    45961 caagtctggc caatgttcgt tcagccatga atgacatgga aagtgtcgaa gagtatgacc
    46021 ctgaataccg tgaaatttca agctctctgt ctgagaccta ctatgtttta gaagacatta
    46081 gcaaacgttt ggaagctatc attgaggacc ttgattttga tggcaatcgc ctcatgcagg
    46141 ttgagaatcg tttggacctc cttcatacta ttactcgtaa gtatggtggg actgttgatg
    46201 atgttttgct ttattttgcc aagattacgg aagaatacaa tctcttgaca ggcaataatc
    46261 tttcgtctga ggacatggaa gcagagctta agaagttgga agtcaatctt gtcaatttgg
    46321 caggtcaact tgcttctgct cgtcataatt tggctcagca actcgaagct gagattaaac
    46381 aagaactgca agatctttat atggaaaaag cccagtttca ggttcgtttt agtaagggaa
    46441 aattcagtcg tgagggaaat aaaatggttg agttttatat ttcaaccaac cctggagaag
    46501 actttaaacc cttggttaag gttgcttctg gaggggaatt atctcgtctc atgttagcca
    46561 ttaagtctgc cttttcacgt aaagaaggca agactagcat tgtctttgat gaggtggata
    46621 cgggagtttc aggtcgtgtt gctcaagcta ttgctcagaa aattcataaa attggtcagc
    46681 atggtcaggt tttggctatc tcccatttgc cacaagtaat tgcgattgca gattatcaat
    46741 tctttattga gaagattagt aatgaccatt caacggtttc gactgttcgt ctcttgacgg
    46801 tcgaagagcg agtggaggaa gttgccaaga tgttggcagg tgatgatgtg acagaagcag
    46861 ccctgacgca agcaagagaa ttgttgagaa acagggagaa ataagatgac agattattat
    46921 gtaattggag atgttcacgg aaaagctggg atgctggaag accttctcaa aacctgggat
    46981 ggtcagcccc agttgctctt tctaggtgat ttgattgaca gaggtgagga tagtcaccgt
    47041 gttcttgaaa tggtcaagga cttggtggac aatcaagggg ctatctgttt gtccggaaac
    47101 cacgagtata tgtttttgac ttggctagat gacccagaag aaagttatga ccattatagt
    47161 cgcaatggtg gagatacaac cattaactct atcctaggtc gtcccttgga tgcaccagtt
    47221 gatggagtag aagatgccaa gcgggttgcc gctgaagcgg cagacttggt cgaattcatt
    47281 cgtcaaatgc catttgtagt agagacagac aagtatatct ttgttcacgc aggtattgat
    47341 ttgaccttgg acgactggca tgaaacaaca gattataaaa aagtatggct tagaaaacca
    47401 ttccacggag ctgaaaatca tactggaaaa accattgtct tcggtcatac accggtctat
    47461 ggtttattac agcaagaacg cggtacatct gagctttgga ctacagaaga tggcaagatt
    47521 ggaatggatg gaggagctgt ttatggtggt gtccttcacg ggattgtctt tacagaccaa
    47581 ggaatgacag aacaccactt tatcgaaaat gacggctttg tcgctgaaga ttagtactcc
    47641 tagcagggta tggtcttgtc aaaatatcaa aaacaattta taataaatag ataccctgaa
    47701 aggaagagaa tcatgaactt agaagaattg aaaaaacgac aggagaagat ccgtaacttc
    47761 tctattatcg cccatattga ccacggaaaa tcaactctag cagaccgcat tttggaaaaa
    47821 acagagacgg tttcaagtcg tgaaatgcag gcccagcttt tggatagcat ggagctagag
    47881 cgggaacgtg gaattactat taagttgaat gccatcgagt tgaattacac tgcaaaagat
    47941 ggggaaactt atattttcca cttgattgac acaccagggc acgttgactt tacctatgaa
    48001 gtttcacgtt cgctagctgc ctgtgagggt gctattttgg tggtcgatgc ggctcaagga
    48061 attgaggctc aaactcttgc caacgtttat ctggctttgg ataatgattt ggaaatcatg
    48121 cctatcatta ataaaattga cctgccggct gcagatccgg agcgcgtgcg tacagagatt
    48181 gaagatgtga ttggtttgga tgccagtgaa gcagttttgg cttctgccaa ggctggtatt
    48241 gggattgaag aaatcctcga gcaaattgta gaaaaagtac cagcaccaac gggtgatgtg
    48301 acggcgccac ttaaggcctt gattttcgac tctgtttacg atgcttaccg tggggttatc
    48361 ctccaagtgc gtgtcatgga cggagtggtc aaacctggtg ataagattca gctcatgagc
    48421 aatagtaaga cctttgatgt ggccgaagtc ggtattttta caccaaaagc ggttggtcgt
    48481 gatttccttg cgactggtga tgttggttac attgcggcgt ctatcaagac ggttcaggat
    48541 actcgtgtgg gtgataccgt taccttggca accaatcctg cggcagaacc attacatggt
    48601 tataagcaga tgaatcctat ggtctttgcg ggtctctacc caatcgaatc aaacaagtac
    48661 aatgacctgc gtgaagcgct tgaaaaattg caactgaatg atgctagtct tcagtttgaa
    48721 ccagaaacat ctcaggcact tggatttggt ttccgttgtg gatttcttgg acttctccat
    48781 atggatgtta tccaggaacg tttagagcgt gagttcaata ttgacctcat catgacagct
    48841 ccgtctgtta tttacaaagt taatttgacc gacggtgagt ctatggatgt gtctaaccca
    48901 tctgagtttc cagacccaac taagattgcg accattgaag agccttatgt caaggcgcaa
    48961 atcatggtac cacaggagtt cgtcggagca gtaatggagc tagctcagcg taagcgtggg
    49021 gactttgtga ctatggacta tattgatgat aaccgtgtca atgttatcta tcaaattcct
    49081 cttgctgaaa ttgtctttga cttctttgat aaacttaagt cttcgacacg tggttatgca
    49141 agctttgact acgaattgtc agaatatcgc ccatctaagc tggtgaaaat ggatattctt
    49201 ctcaatggag ataaggtgga tgccctcagc tttatcgttc acaaggactt tgcctacgaa
    49261 cgtgggaaac tcatcgttga taaactcaag aaaatcatcc ctcgccaaca atttgaagtt
    49321 ccaatccaag cggctattgg acacaagatt gtcgctcgta ctgatatcaa ggcccttcgt
    49381 aagaacgtac ttgctaaatg ttatggtggt gacgtttctc gtaagcgtaa actccttgaa
    49441 aaacaaaaag ctggtaagaa acgcatgaaa tccatcggat cagttgaagt tccgcaagaa
    49501 gccttcctca gcgtcttgag tatggatgaa gaataagtta tcagaaatgc ctttccttgt
    49561 tcacaggaaa tttataagaa tcaatagtaa agcaggctta gatggtctgc ttttttagtt
    49621 tttatactct tcgaaaatct cttcaaacca cgtcagcgtc gccttaccgt atgtatggtt
    49681 actgacttcg tcagtttcat ctacaacctc aaaaccatgt tttgagctga cttcgtcagt
    49741 ttcatctaca acctcaaaac tgtgttttga acaacctgcg gctagcttcc tagtttgctc
    49801 tttgattttc attgagtatt agaacagaaa aaatgcttgg agtatttgtt tgtgtgttta
    49861 cttttatata acaaactata aacacaacaa aaatataaaa aaagagacaa aaaagaacag
    49921 aaagtaatta acaacgattt catatagtgt tatacttata gcataaagtt aatggaaaga
    49981 agggaaaacc ttatggtatt agattatttc tttgacaaaa accttgtgtt ttgcttagaa
    50041 gcggataatc aagaataact ctttgatcag gtagcaagct tgttggagga acgagaaatt
    50101 gt
//
LOCUS       DAVBBS010000007        48474 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVBBS010000007 DAVBBS010000000
VERSION     DAVBBS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48474
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.007"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(308..1474)
                     /gene="recA"
                     /locus_tag="VQU32_000401"
     CDS             complement(308..1474)
                     /gene="recA"
                     /locus_tag="VQU32_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET2471919.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            complement(1529..2785)
                     /locus_tag="VQU32_000402"
     CDS             complement(1529..2785)
                     /locus_tag="VQU32_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET2471920.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNLLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQVDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGISSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            complement(2869..3885)
                     /locus_tag="VQU32_000403"
     CDS             complement(2869..3885)
                     /locus_tag="VQU32_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET2471921.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEKALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            complement(3893..4411)
                     /locus_tag="VQU32_000404"
     CDS             complement(3893..4411)
                     /locus_tag="VQU32_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2471922.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            complement(4401..4844)
                     /gene="tsaE"
                     /locus_tag="VQU32_000405"
     CDS             complement(4401..4844)
                     /gene="tsaE"
                     /locus_tag="VQU32_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET2471923.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            complement(4930..5550)
                     /locus_tag="VQU32_000406"
     CDS             complement(4930..5550)
                     /locus_tag="VQU32_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471924.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            complement(5933..6796)
                     /locus_tag="VQU32_000407"
                     /pseudo
     CDS             complement(5933..6796)
                     /locus_tag="VQU32_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            6961..7155
                     /locus_tag="VQU32_000408"
     CDS             6961..7155
                     /locus_tag="VQU32_000408"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471925.1"
                     /translation="MKKKRVSRMKKNRGIQKLAILVLLGVFMFSNTIPYQQFIQKNRQ
                     LEIRVQSQKKSNGLDVGKAD"
     gene            7470..7766
                     /locus_tag="VQU32_000409"
     CDS             7470..7766
                     /locus_tag="VQU32_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET2471926.1"
                     /translation="MLKFRKVVASSLAVLAFSLALPVLAVSHRGGEWTYGGHHDPNNW
                     GAFSNYYHGSQYHWAYVGSNGRNNQKTVYAGARSAAYAFVNTNFGEQVTFDAGW"
     gene            7869..9884
                     /locus_tag="VQU32_000410"
     CDS             7869..9884
                     /locus_tag="VQU32_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2471927.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSYYYPNVVVHDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKKLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMNLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFIALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAGLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            9886..10518
                     /locus_tag="VQU32_000411"
     CDS             9886..10518
                     /locus_tag="VQU32_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2471928.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLTYLDLDKRIFELSGGESQRVALAKVILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDRLK"
     gene            10659..10937
                     /locus_tag="VQU32_000412"
     CDS             10659..10937
                     /locus_tag="VQU32_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HET2471929.1"
                     /translation="MKKFDNYIVEKPCDSNSDKLQKILIIENLVDDILQFPLRINNSV
                     GEIFLLQPFQKKTIFIPCYFEEDIVKVKDDDKVEWNLLEFQKFRAFLA"
     gene            complement(11446..11859)
                     /gene="ndk"
                     /locus_tag="VQU32_000413"
     CDS             complement(11446..11859)
                     /gene="ndk"
                     /locus_tag="VQU32_000413"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET2471930.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(11970..15647)
                     /gene="rpoC"
                     /locus_tag="VQU32_000414"
     CDS             complement(11970..15647)
                     /gene="rpoC"
                     /locus_tag="VQU32_000414"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET2471931.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGEVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPYAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(15680..19291)
                     /gene="rpoB"
                     /locus_tag="VQU32_000415"
     CDS             complement(15680..19291)
                     /gene="rpoB"
                     /locus_tag="VQU32_000415"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET2471932.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSSLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            19707..19777
                     /locus_tag="VQU32_000416"
     tRNA            19707..19777
                     /locus_tag="VQU32_000416"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19739..19741,aa:Cys,seq:gca)
     gene            complement(19975..21306)
                     /locus_tag="VQU32_000417"
     CDS             complement(19975..21306)
                     /locus_tag="VQU32_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HET2471933.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFNVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(21597..22421)
                     /gene="endA"
                     /locus_tag="VQU32_000418"
     CDS             complement(21597..22421)
                     /gene="endA"
                     /locus_tag="VQU32_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HET2471934.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSTANSPKTKLSQKKQ
                     APEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(22460..22648)
                     /locus_tag="VQU32_000419"
     CDS             complement(22460..22648)
                     /locus_tag="VQU32_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET2471935.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDSW
                     AILSPAKWQELIHKFTGN"
     gene            complement(22641..23924)
                     /gene="murA"
                     /locus_tag="VQU32_000420"
     CDS             complement(22641..23924)
                     /gene="murA"
                     /locus_tag="VQU32_000420"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET2471936.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(23948..24178)
                     /locus_tag="VQU32_000421"
     CDS             complement(23948..24178)
                     /locus_tag="VQU32_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HET2471937.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTAENTRKVRV
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(24236..25273)
                     /locus_tag="VQU32_000422"
     CDS             complement(24236..25273)
                     /locus_tag="VQU32_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET2471938.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(25257..25745)
                     /gene="coaD"
                     /locus_tag="VQU32_000423"
     CDS             complement(25257..25745)
                     /gene="coaD"
                     /locus_tag="VQU32_000423"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET2471939.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLCVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            complement(25735..26274)
                     /gene="rsmD"
                     /locus_tag="VQU32_000424"
     CDS             complement(25735..26274)
                     /gene="rsmD"
                     /locus_tag="VQU32_000424"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET2471940.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEVGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKTVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(26339..27331)
                     /locus_tag="VQU32_000425"
     CDS             complement(26339..27331)
                     /locus_tag="VQU32_000425"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HET2471941.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(27443..28126)
                     /locus_tag="VQU32_000426"
     CDS             complement(27443..28126)
                     /locus_tag="VQU32_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HET2471942.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(28182..28922)
                     /locus_tag="VQU32_000427"
     CDS             complement(28182..28922)
                     /locus_tag="VQU32_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET2471943.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRNQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            28956..29234
                     /locus_tag="VQU32_000428"
     CDS             28956..29234
                     /locus_tag="VQU32_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET2471944.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            29312..30238
                     /gene="yidC"
                     /locus_tag="VQU32_000429"
     CDS             29312..30238
                     /gene="yidC"
                     /locus_tag="VQU32_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET2471945.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(30313..31107)
                     /gene="pflA"
                     /locus_tag="VQU32_000430"
     CDS             complement(30313..31107)
                     /gene="pflA"
                     /locus_tag="VQU32_000430"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HET2471946.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(31233..32483)
                     /locus_tag="VQU32_000431"
     CDS             complement(31233..32483)
                     /locus_tag="VQU32_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET2471947.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(32550..33377)
                     /gene="purR"
                     /locus_tag="VQU32_000432"
     CDS             complement(32550..33377)
                     /gene="purR"
                     /locus_tag="VQU32_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HET2471948.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLHEFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(33644..34585)
                     /locus_tag="VQU32_000433"
     CDS             complement(33644..34585)
                     /locus_tag="VQU32_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HET2471949.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            complement(34575..35831)
                     /locus_tag="VQU32_000434"
     CDS             complement(34575..35831)
                     /locus_tag="VQU32_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET2471950.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(35833..36495)
                     /locus_tag="VQU32_000435"
     CDS             complement(35833..36495)
                     /locus_tag="VQU32_000435"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HET2471951.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDGFVGVDRGSLWILE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFKQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLASYMHQIEIEDGQNLITYCPEGISQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(36458..37114)
                     /locus_tag="VQU32_000436"
     CDS             complement(36458..37114)
                     /locus_tag="VQU32_000436"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET2471952.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDGHFVP
                     QISFGAGVVEALRPHSKMVFDCHLMVSNPEHHLEDFVRAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALRDEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            complement(37125..38003)
                     /gene="rsgA"
                     /locus_tag="VQU32_000437"
     CDS             complement(37125..38003)
                     /gene="rsgA"
                     /locus_tag="VQU32_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET2471953.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDGGELDFYQQTYGDIGYDFVTSKEELLPLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     TAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGTIAPFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(38596..39369)
                     /locus_tag="VQU32_000438"
     CDS             complement(38596..39369)
                     /locus_tag="VQU32_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HET2471954.1"
                     /translation="MRKKLVNSLMCAVVLSGFPIATYANEKNIGPNITVSNGQTRLSI
                     PEKNGVEVIQSEENRVVYEVKEAAKIETSDGDMVAPIVVDSRITSINNQVVQGKTVYT
                     ADLEKATFVEEGYNKLAKPTNILDHIFGIQKVHAYTQNITTHQSQFDSTYSVELYTVV
                     HWVSYDGGVQKNITKISGGVDFHERDIEIESSSVEVRQGGWYSSQQKWFHPGARSSWE
                     YSTGFSKVGPGENRVVTNYTVNLIRYGDRWSAYLQNNPW"
     gene            complement(40059..40931)
                     /gene="rsmA"
                     /locus_tag="VQU32_000439"
     CDS             complement(40059..40931)
                     /gene="rsmA"
                     /locus_tag="VQU32_000439"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET2471955.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAEVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPVPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLE
                     EFASLADALKGQGL"
     gene            complement(41182..41400)
                     /locus_tag="VQU32_000440"
     CDS             complement(41182..41400)
                     /locus_tag="VQU32_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2471956.1"
                     /translation="MENMTNPLLLVQIRVHGYLLDFASSEGQRQKHYRLKNLPQTVEL
                     TVDDVEEDVDLTLPENRSYQEADFFDTQ"
     gene            complement(41468..42109)
                     /locus_tag="VQU32_000441"
     CDS             complement(41468..42109)
                     /locus_tag="VQU32_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2471957.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(42111..44141)
                     /locus_tag="VQU32_000442"
     CDS             complement(42111..44141)
                     /locus_tag="VQU32_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2471958.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEVLSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQFICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSTYYQRLFTDYL
                     QNFSSESVEVTSQKYYLPQVSLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(44410..45309)
                     /locus_tag="VQU32_000443"
     CDS             complement(44410..45309)
                     /locus_tag="VQU32_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2471959.1"
                     /translation="MLIGQKIKEIRIEKGVSRPYFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPPAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(45381..45941)
                     /gene="rnmV"
                     /locus_tag="VQU32_000444"
     CDS             complement(45381..45941)
                     /gene="rnmV"
                     /locus_tag="VQU32_000444"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HET2471960.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINAQDIERI
                     QRLHQRQGVIVFTDPDFNGERIRRMIMTAIPTVRHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(45941..46714)
                     /locus_tag="VQU32_000445"
     CDS             complement(45941..46714)
                     /locus_tag="VQU32_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET2471961.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     RALELVDEYDQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKVTDLQEAAKELPLDKILVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(47019..47912)
                     /locus_tag="VQU32_000446"
     CDS             complement(47019..47912)
                     /locus_tag="VQU32_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HET2471962.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLENTPGGDKGGNTGSSDANANAGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIVMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            complement(48196..48330)
                     /gene="rpmH"
                     /locus_tag="VQU32_000447"
     CDS             complement(48196..48330)
                     /gene="rpmH"
                     /locus_tag="VQU32_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET2471963.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
BASE COUNT        13990 a        10915 c         9042 g        14527 t
ORIGIN      
        1 taaagctatt atatcagctt tccagaggag cgacaagaaa cagcaaactt atatatagcg
       61 gaatgaaata aggaaagtca aatcgatttt taacaatgtt ttagaagtag atatgtacta
      121 ttctagtttc gatctattat agttggttga gtttggaata gtacgctgta gctgctaaaa
      181 catttctaga aattaatttg actttcctaa tagagttgtt catatcttat ttcaagctac
      241 tatatataat ctaaaaactt gaatcaaaaa tcgaaaaagt agcggatttc tccactgctt
      301 taacagctta ttcttcaatt tcgatttcaa gttcatcgcc taagtcaagc ggaacttctt
      361 cattcactgc ttcttctttc tttggctcat cttttttgtt ttcagtatct tgttctgaaa
      421 cttcttctcc atcaatcaag ccaaatttag aacggacttg cttatcaatt tcatcaaaga
      481 tttctgggtg ctctgccaag tatttcttag cattctcaga accttgccca attttttcat
      541 ctttgtaaga ataccaagcc cctgcttttt tgataatatc caaatcgctt gcaatcttca
      601 aaagctcacc agtcttagaa attccttctc cgtacataat ttcaactacg gcttccttaa
      661 acggtggagc taccttattt tttacaacct taatcttagt ttctttaccg acattggttt
      721 ctttttggtc accagttccc ttaatttgtg tattaccacg aacatccaag cggactgaag
      781 catagaattt caaagcacgt ccgcccggtg ttgtttctgg atttccaaac atcactccaa
      841 ctttttcacg caattggttg ataaaaatgg caattgtttt ggttttattg atagaggcgc
      901 caagtttacg catggcctgg ctcatcatac gagcctgcaa accaacatgg ctatctccga
      961 tatctccatc aatttccgca cgaggaacaa gggcagcaac tgagtcgact acgacaagat
     1021 caactgcacc tgagtcaatc aattttcccg caatctcaag accttgctct cctgagtctg
     1081 gttgagacaa gagcaattcg tcaatattga caccaagggc cgcagcataa gctggatcaa
     1141 gggcatgttc cgcatcgata aaagcagcaa tcccaccttc tttttgcgct tgtgcaactg
     1201 catgaagggc aaccgttgtc ttaccagatg actctgggcc atagatttcg atgatacgtc
     1261 ccttaggata accacctgag ccaagggcaa tgtcaagagc taaagaacct gagctcatca
     1321 cttgcacctt ttgctccgca cgttcaccca aacgcatgat tgatccttta ccaaagtctt
     1381 tctcaatcaa tttaagagcg tcattcaagg ccttttcacg ttctgcccca aatttttttg
     1441 aaatttcttc taattttttt ggttttttcg ccattctatt ctcctacatt ctaatgatcc
     1501 tcagacctat ctactattat atcaaaaatt agtcacttaa taaagccttg cgaactaggt
     1561 taaaggcatg cataaccgca atgtgacgta catctgctcg gcttctgcct ccaatattaa
     1621 ccttgataac ctcagttcct tgatcttgcg ccaagcctat gaagactgtc ccaactgggt
     1681 gtccttctag gctatctggt cctgccactc cagtcaaact aattccaaaa tcagactggg
     1741 tcttgcttcg tgcctgctca gccatcttct gagctgtaaa ttcagacacc acaccatgtt
     1801 cttccaaatt cttggcagga atatccaaca tccttgattt ttcctccaag ctataggtca
     1861 caaaaccacc cttaaatata cttgaaattc cagaaaaatt cgccacggta gcttggaaaa
     1921 gacctgccgt caaactctct gcagccgcga tggttttccc ttgccttttc agttcttcta
     1981 ccacaatgct ggctaaacta gtttcttccc cataaccata gcaaaagtct cgtaaagaaa
     2041 ttccttcgaa agtctggcag tccaagattt gattttccaa gatatccagc gcttgattcg
     2101 cctcttcttg actgctagcc tttgttgaca gacgtagagt gacttctcct gtcttggcat
     2161 aaggggccaa ggtaggatcg acctgattat caattaaatc agccaaaatc gtaaccagct
     2221 ggctctcgcc aatcccaaag aaacgaagaa ctcgggaata cagcttgctc cctgtcatca
     2281 acttgggtag aagtaggttt aagaccatgg gtttcaattc acttggcgga cctggaagga
     2341 cgacataggt cactccgtct acttctaatt ttcctccaac agccagtcct gtttcgtttg
     2401 gcagtggaat cgctccttct acaatttgag cttgtctttc gttattcggt gttcgggcat
     2461 agtctggtcg cagggcaaaa aagatatcca acttctcctg agcctgagga tcaaagacta
     2521 atgctttccc taaaaattta gctagggttt gtttggttag gtcgtcctca gttggcccca
     2581 aaccgcctgt caaaatcacc agactgctac gttgactggc aatctcaagc aaagacaaga
     2641 gacgaacttc attgtctcct acagccgtct gaaaatatac atctacccca atctcagcta
     2701 gtttttccga caaaaactgg gcattggtgt tgacaatctg tcctgtcaaa atctctgttc
     2761 caacagcaat gatttctgct ttcatgtttc ctcctaccta tctattcgta tttttttgaa
     2821 aaaatcgcag gaattttcct acgattgatt ttttatttgt atcaaaagtt aattatcttc
     2881 atcaccaaca ggtgctctgc caaataaatc ttcaaataaa accgcattgg tttcaagctg
     2941 agtaacttct tcttgtccca aagaacgtcg gagtatattt tgcatttcca acatatgtgc
     3001 tcttgaaaca atctggtaag aaacaccttg aagtatctct ccttcaccct gcaactgctg
     3061 agtttcaatg gttttaaatg aatctttata gcctagcaag ttagggatac tttttgcaga
     3121 caaatcaata ttggtctgca tattgtcact caaagctttt agaatctctt gataatgacc
     3181 aacgctattt aaactgagag ctttttccat gactttttga ataacttcac gttgacgttt
     3241 ttgacgacca taatccccct caggatcttg gtaacgcatt cgtgcataga ctagggcttt
     3301 ttctccccca atatgttgct ccccaacacc gatagaaata gtattaaatt cttcttggtc
     3361 actgatagaa attgggaaac ctaggatatt attgactgta atacctccta ctgcatccac
     3421 tagtttttgc aaccctctca tattgaccat cacatagcga tcaatatgga tattcatcat
     3481 tttttgaatg gtttctatag caagctctgc tccaccatct gcatatgctg agttcagttt
     3541 cgcttcatga gcctgaccat tccctgattc aatgcgcgtc agaatatccc gctctaaact
     3601 catcattgtt gtttttttcg ttttaggatt cactgtcatc aagatcatgc tatcacttct
     3661 accgacccaa gtttcagttc gttcaacatt tccggtgtcc actcccatta acagaatggt
     3721 tagaggttca gtcgcttcaa taaccttggt ttcttcgccg atttttttat aggttttagc
     3781 taaggtttct gtcccttgtt gataaatagt ataagcaaaa acacctactc ctactacagt
     3841 tacagaaagt aaagctagca ccattccaat aattttttta accatatttc tactaaccta
     3901 tcagtttacc catcaagtaa acatcgataa atttcccttc ttctatatat gccccacgct
     3961 cttggctacc ttcaatgaca aagccatgct tttgataaag atggactgct gcttgattac
     4021 gagtttggac agtcagttgg agacgacgca gaatgccact tgcttgtgcc cactctatcg
     4081 cttcttctag caacaaactt cccaagccat tattccaata tctttttcca atcacaatga
     4141 agagatctcc aatatgacgg actctcttac gctgatcagc tgtaatattt acaataccag
     4201 caattttgcc atttaagaat gcaagtaagg ttatctgatt gtccgaacta gcttgcttgt
     4261 tgaggaatat ttccatctcc tcactagtca agagaatacc atctccgtct aggctggtaa
     4321 agtctgtctc caaactcaca cgatttaaaa aggccactaa ttcagctgca tctttgggct
     4381 ctgcttccct aatgagcaat tcatactcca tattgaagct cctctaacaa tttctcagca
     4441 cgcaaaccct ttgcctgaaa atttaaacgg cgtccatctg cttcttttag aatttccaat
     4501 tctaaataag catctggcaa ggcatctcct aagagatttc cccactcaat aacagtcacg
     4561 ccgccaccaa agataaactc atccaagtcg atagaatcag catctccttc aatacgataa
     4621 acatctaggt gataaagtgg aagtcgacct tcatactctc tcacgatagt ataggtggga
     4681 cttttaatca tttgagaaat ctgtaatcct tttgcaagtc ctttagtaaa ggtcgtttta
     4741 cctgcaccca gttctccagt taagattaaa acatcattct ttgctaatag atggcccaaa
     4801 cgctccccta aggcttgcaa ctcttcttca ttttttgtgt acatactctt attataccaa
     4861 aaacttttct tttgtgtcta ttttcctact aaacttatca tcataacatc cataaaaaac
     4921 aggctttctc taaaagaaaa tgagcgtaac aatgaccaat acaagatctc ggaaaatatg
     4981 accataaaag gaaacttcct tcttaaccga atttgggaca agataggctg caaaaaacaa
     5041 gcccagtcca atataaatca gaagtgagac aatggtcatt ggatttctta agaaaagaag
     5101 tgttgctaaa atagtcacca acactgtctt ttttctgtct agcatagcaa gaaaatcgcg
     5161 cacgtatttt ttcaagggta aaaaaatcag caaatctagc ccaaatagga aaaagaagga
     5221 tggcaataaa aagtcaacta attcttgctg cagcgtattt ttgatgaaca agttatctga
     5281 caaaacaaga acagctccta acaaattaat taagagtaac atactgtaaa aaagcttcac
     5341 cgacttctta ctggctagga cactatggac ttcttgctta cgggtataaa gataatttac
     5401 tccagcacag attcctgaaa cgaaaaccat gctcccgatg aaaaaagctg tactttgttt
     5461 aaaggacaag atgcattcct tccataggaa acagctactc aaactgattt gaattaaagc
     5521 taacaaaaat aagattctca ttgatttcat cttctctctc ccttcctacc aatcattata
     5581 ctaggagaaa agagagaact gtttctaatc ttctcaaatg tctctttaag acgctaaaca
     5641 aacactagag actaatactc aatgaaaatc aaagagcaaa ctaggtagct agccacaggt
     5701 tgctcaaaac actgttttga gattgcagat agagctgacg tgatttgaag agattttcga
     5761 agaatataaa tttgaaatca tgaaaatccg tcaaacgggt ggttgttttg tctcgcacct
     5821 cacggagcga gacggactca gagtcacata attataaggc tgatagtatt aatctaacta
     5881 tcagccttac aggttattta acgtttcaga aaaactataa tgtcaagatt aactaaacag
     5941 tatctaattc cttcaaataa ttttctatct tcatcagcat taaaggattg ttataaatct
     6001 tgcatagctc tcttgcttct atataataat ttttgacttg ttctctgtct agaaatttgg
     6061 ctccagcatt tcctacaaga ataagtagag gagccaattg gtagcttgtc tgtctttgtt
     6121 tacagagttc gatagtctca agagcttctt ggatggcttc attatatttt tcctttgata
     6181 ctaggtagtg agcgtaattg taacgaactc tgatgtagcc aaataaaaac tcttgatgct
     6241 caaaattttt tgtctgatat aactccatca aatgagagta gtttacctca tattcttgtt
     6301 cacgacccac taaggaatag aaattagata gagtattcaa cgcctttaaa taaatcaaag
     6361 tatttgagga gacttttaat aatatatttt ccaatgaaga aattgcttca cacttactgt
     6421 catattgaca gaagtcaata atagctttaa tccattcaag gtaagttcgg tcttctagtg
     6481 ttagaaaagt acttcgttca atctctattc tataaatata ttccaaattt tcataatttc
     6541 tatcatctaa taggcgagca gataattgct tgaaattaga gaggttagat ttaatttcaa
     6601 tttgttcatt aaaaaaataa tctaatggca cttcgagtcg ttgtgagagt ttgaacaaaa
     6661 ggtctgcgga gggaatgaaa taccctctct caattttact aatctggctc tattcacaaa
     6721 ttccttctgc aagagtttgt tgggagagtc tcaactcttt tcttttcaat ctgaatttct
     6781 ctgcgagtgt attcattaaa gataataaac cttcctattt gcttaatttc attataaaat
     6841 ttttagttca aaatagcaag ttaaaggaat aaataattct ttgcggtaat aaacaaaaaa
     6901 aagtcaaaaa ttacataaaa atgcttggct aaataaaata tatttgttaa aataaggata
     6961 atgaaaaaga aaagggtttc acgaatgaag aaaaatcgtg gaattcaaaa attagctata
     7021 ttagtattac taggtgtttt tatgtttagt aacacaattc cttaccaaca gtttattcag
     7081 aagaatagac aattggagat tcgagtccaa tcgcaaaaga agtccaatgg tcttgatgtt
     7141 gggaaggctg attgatttta tattacttta aaattcaact atttagagaa aggtattctg
     7201 atgagagctt gtaacaatac cagatagata taagaatgga gatgtttgat aaaatgcttt
     7261 catagctaac caactgttga aaattatgag ttacaaaatc taaagtattt aagattggtt
     7321 tataaaatta ggtctctgcc tgtagaaagg aacgtgcagt tagaaaggag gaaaaaatag
     7381 gatactataa ttcagactga gatttctaat taaaatatat agttgcattt cgctcatttt
     7441 cataatattt cattcagagg agtaataata tgttaaaatt tagaaaagta gttgcatcat
     7501 cccttgccgt tttggcattt agtttggctc ttcctgtttt ggcagtttct caccgtggtg
     7561 gcgagtggac ctatggaggc catcatgatc caaataattg gggagcattt tcgaattatt
     7621 atcatggttc acagtatcac tgggcctatg ttggaagtaa tggacgtaac aatcaaaaaa
     7681 cagtttatgc aggagcacgt agtgcggcct atgcgtttgt taatacaaat tttggggagc
     7741 aagttacatt tgatgcggga tggtaaagaa taattgaaga agattctttt atcaatagtt
     7801 cagtaagctt gttagtttat agagggcagg ggtatttctg ccctctgttt tagaataagg
     7861 aatgcactat gaaaaaatta tttatactca tatccaacct gcttgctagt ttgttctttg
     7921 tttgggtatt taccatttgg actgatacat atgtctccta ttattacccc aacgttgttg
     7981 tacatgattc ttcccctgaa acaacttttc agcatgttgc tacacgcttg gaaaaactag
     8041 cagaagaaac agatagcttt attgcgattc aacaccaaga tcctaactca gaaggtacta
     8101 cagttttttc ttatacgacc tttggggacg gaaagttgcc tgatgggctt caggaaaaaa
     8161 aactagaaga tgctcaaagt agtagtgttg aaacaaacta ttttgtattc gatggacacc
     8221 tagatattca cttgctaagg gaggagctaa gtcaacttgg cttgactaac atgaacctga
     8281 caattccatc taaactatct acgttaatgg ctatctttag taacggattt cagttaatca
     8341 gtctattgat tttcatctta acctttatag cactgacttt aattagtcaa attagccaat
     8401 tacggtcgtc gggcattcgc ctcatttcag gagagaaacg gtggtccatt ttccttagac
     8461 cagttggtga ggatttgaaa gggatagctg ttggtttcag tttagctggc gtgctcgcca
     8521 ttcttatgca aaaaattctg tcgcttccaa cgcaatccct aatgaccata ggagcaggct
     8581 tgctcagtta taatctcatc ttgttgtcga tctccctgtt tttcgctcaa ctcttcgcag
     8641 ttgggataaa aaagatacac ctgatgcaga ttataaaagg gcaagtgcct gtcagaggaa
     8701 tcattagttt gattttaatc ggtcaactat tagcgattat tattgtaacg ctgggaatag
     8761 ggagtagctt aaagtattcc caagcctggc aacagcatcg gattggacaa gaggcttgga
     8821 gtcaggaaag gcaactgatt accctatcaa tcagccgtga aggaacgagt cctggttttg
     8881 atgaacaagc tcaaaggaaa ctcagaactt ggtatcaatt gatggatctg gctgtttcag
     8941 aacaaaaggc tttcttatct agacaccagt taattgaccg tactttgcaa aatggcatgg
     9001 cttcctccaa aaacttgata acctctacag aatggcacga ttacaacccg aatggcaatg
     9061 tccttatcgt cacaccgcaa tacttggagc gtcaaaacat tcctgtagat acaactattg
     9121 agcaaaagat gaatcacctt gatgtagggg agtttgtctt attgctgcct gaacacctcc
     9181 gttcagagga agaacattat aaatctgttt ttgaagacga cttaaccagt cgcatgtcta
     9241 gtcaagatga acgacagcaa atgactgcta cggtaggtta tttagaatca ggtcaggatc
     9301 gttttgtgta taatacgacc cctatttctt accagcagtt tttgaaagat ccaatcatca
     9361 ttgttataac accccaatca actggtccac agtccatttt gttttggata gacgcagtac
     9421 agaactacgt tctctttaat caattgtctg atgcccagga gcttatccag agacaaggca
     9481 ttgaaaattg ggtctcagaa atgcaaacag gttaccacaa ctacatcaca ttattggata
     9541 atattcagag ggaacgttgg gtaatgctag caggagctgg gcttgggatt gcaacttcaa
     9601 tcttgttgtt taacactatg aataggctct actttgaaga atttagacgt gccattttta
     9661 tcaaacgcat tgcaggtctc aggttcttag aaatccatcg cacttatctc tttgctcaac
     9721 tgggtgtgtt tttactggga tttgttgcga gtgtatttct tcaggtagag ataggagttg
     9781 ctttcttagt cttgttactc tttactggtc tatctctttt acagttacat gtccaaatgc
     9841 agaaagaaaa caagatgtcc atgcttgttt tgaagggagg ttaatatgat tgaacttaaa
     9901 caggtgagta aatcttttgg agaacgagag ttattttcga atctttcaat gacatttgag
     9961 gctggaaaag tctatgcctt aattggttca agtggtagcg gaaaaacaac cttgatgaac
    10021 atgattggga aattagaacc ttatgatggg acgatttttt accgaggtaa agacttggcc
    10081 aattataaat cgagtgattt tttccgtcac gaattgggct acctcttcca gaactttggc
    10141 ttaattgaaa accaaagtat tgaagaaaac cttaagctag gtctcattgg tcaaaagttg
    10201 agtcggtcgg aacagcggtt gaggcagaag caggctttag aacaggtcgg cctgacttat
    10261 cttgacctag ataagcgcat ctttgagtta tcgggcggag aatcgcaacg ggttgccttg
    10321 gcaaaagtta tcttaaagaa tccacccttt attctggcag atgagccaac agcttcaata
    10381 gacccagcaa cttctcagtt gattatggag attctgctat ctcttcgaga tgataatagg
    10441 ctaatcatta tcgcaacaca taatccagca atttgggaga tggctgatga agtgttcacg
    10501 atggatcgtc tgaaataaaa atccttgttt ttaattgcac gatgagttac tgaaatatta
    10561 tcatgaatca ataattggag ttaatttaga attgtacttt attaatattg aggtaacttt
    10621 ttcttgataa aggaagaaat agtggagagg aagttagaat gaaaaaattc gacaattata
    10681 ttgttgagaa gccttgcgat tctaattcag ataaactgca aaaaatctta ataattgaaa
    10741 atttggtaga tgatattttg caatttcctc tcagaatcaa taatagtgta ggagagattt
    10801 tcctcctaca accgtttcaa aagaaaacta tctttattcc atgttatttt gaggaagata
    10861 ttgtgaaagt caaagatgat gataaagttg agtggaattt gttagaattt caaaaattta
    10921 gagcattttt ggcttagtaa tctgtgttga aggctcaaaa cctatggtaa aaaagtcgct
    10981 ttgaaaacgt attgcctcca aagatttagt taaataatga tttaacacaa aaagaaatta
    11041 ttgaagttct ggaaagatgt tgtttcagta ttgagaaaag gtgggaaaaa cttgcgattt
    11101 tcacagagaa aggaagaaaa agtatagaaa tatagtcaat tgaaacaaga acaggacaaa
    11161 agaacctttt gtgccatatt tttctccttt cgctttacaa ttggcttgaa cacctttatt
    11221 gtatcgcgtt tggagttttt ttggtataac cttcgacgcg cacccgcata gcgggtgttt
    11281 ttttgtctcg cacctaacgg agcgagacga actaatagtc acttaatcaa aaaacgcacc
    11341 atatcaaaaa ctaaaaagtt tgatatcatg cgtcatgtct taaactaatt gactatactt
    11401 tctattcaaa tgagctttta accaattgat tgagccaatc cactcttaaa accaaagagc
    11461 aatttctcgc ttagctgact cttctgaatc tgaaccatgt acaacatttt ggataacctc
    11521 attttctcca gcagcttttg caaaatcacc tcgaatagtg cctggtaaag cttcttctgg
    11581 acgagttgca cccatcatgg tccgccaagt ttcgattact ttgggaccag aaatgacacc
    11641 cacaagaact ggacctgaag tcatgaattc acgaatcggt gggtaaaaac tctgaccaac
    11701 caagtcctga tagtgctggt caatcaactc ttctgaaact tgtgaacgaa actccaattt
    11761 ttcgattgta aatccacgtt gttcgatgcg ctttaacact tcacccacta gccctctttt
    11821 tacaccatct ggtttgatga taaagaatgt ttgttccata cccgtctcct ttgtcagctt
    11881 ctttctttta ttttaccaca tttcgtggaa aaatggagaa agttttcaga atagagaatg
    11941 agagaacccg agggttctct cattctctat tattctactg tttcttccac agtttcaacg
    12001 gcagtatcca caactacttc tgttgtttct tcatttcctt cttcctctac tggaggatta
    12061 aggtattctt cttcgttgac agcatatggt tcaaggttac ggtaacgggc cataccagta
    12121 ccagctggga tgatcttacc gatgataaca ttttctttaa gtccaaggag atggtctttc
    12181 ttaccacgga tagctgcgtc agtaaggaca cgagttgttt cctggaagga agccgctgac
    12241 aagaaactgt ttgtttcaag tgaggctttg gtaattccca taaggactgg gcgacctgtc
    12301 gctggaactc cacctgcgat aaggacatct ttgttggcat ctgtaaagtc attgatatcc
    12361 atgagggtac ccatgagaag atctgtatca cctggatcca tgacacggac tttacggatc
    12421 atttgacgaa ccattacctc gatgtgtttg tcaccgattt ctaccccttg gctacggtaa
    12481 actttttgta cttcaccgag aaggtacgtt tcaactgaca agacatcacg aactgcaagg
    12541 agacgttttg gttggataga accttctgtc agagcagcac cacgcgctac ttggccccca
    12601 acttcgacac gcatacgagc tgtaaatgga acgacatatt caccttcgcc agtttcaccc
    12661 ttaacaaaga ctttcttggt acgagttgat gcatcttctt cgatagcagt aacttctcct
    12721 ttaacctctg tgataaccgc ttccccttta ggattgcggg cttcaaagat ttcttggaca
    12781 cgaggaagac cctgagtgat atcggtattt gaggcaaccc cacctgtgtg gaaggtacgc
    12841 attgtaagct gtgtaccagg ttccccgata gattgggcag caatggtacc aactgcttca
    12901 ccaacttcaa ccgcatcacc agtcgccaag ttgataccgt aacagtgacg gcagacaccg
    12961 tgacgagtgt tacatgtaaa tacagaacgg atagtcactt cttccacacc agcattgaca
    13021 atttcacgcg ccttgtcttc tgtaatcaat tcatttggac caataatcac tgcaccagtt
    13081 tctggatgtt taacagtttt cttagtgtaa cgaccgttga gacgctcttc gagagactcg
    13141 atcatctctt ttccttctgc gatagaacgg atcaagagac cacggtcagt tccacagtcg
    13201 tcctcacgga tgataacgtc ttgggcaacg tcgaccaaac gacgagtcaa gtaacctgag
    13261 tcggctgtct taagggccgt atcggtcata cctttacgag caccgtgagt tgagaagaac
    13321 atttccaata ccgacaaacc ttcgcggaag tttgaaagga ttggcaattc catgatacgt
    13381 ccattcggag cagccatcag accacgcata ccggcaagct gtgagaagtt tgagatgtta
    13441 ccacgggctc cagagtccat catcataacg attgggttct taggatcttg gttagcaatc
    13501 aagcgtttct caagtttttc acgggcagca cgccattcag ctgtaacagc attgtaacgc
    13561 tcgtcgtctg tgatcatacc acgacggaat tgtttggtga tttgctcgac acgtttgtgt
    13621 gattcttcaa tgatttcagc cttgtcatca acgactggga tatcggcaat acccactgtc
    13681 aatcctgcaa gagttgagtg gtggtaaccg agattcttca tgcggtcaag tagggcagaa
    13741 gtttctgtcg tacggaaacg tttgaagatt tcagcgatga tatttccgag gtttttcttc
    13801 ttgaacggag ggttgagctc aagattgctg atagcttcct tgatatctcc accaagtggc
    13861 aagaagtatt tagctggaac accttctgtc aagttggcat tgtttggttc ttgcaagtat
    13921 ggtagcccct ctggcatgat atcgttgaag agaattttac caactgttgt aagcaagacc
    13981 ttatgtcttt gctcttctgt ccaaggcttg ttgaggctgt ctgttgcgat accaacacgt
    14041 gagtggaggt gaacataacc attgcggtaa gccataaccg cttcgtcacg gtctttgaag
    14101 accattcctt caccttcgcg accagcttct tccatggtca agtagtagtt acccaaaacc
    14161 atgtcctgag atggagtaac taccggtttc ccatctttcg gattcaagat gtgctcagca
    14221 gctagcatga ggatacgagc ttctgcttgt gcttcttctg aaagtggtac gtggatggcc
    14281 atttggtccc cgtcaaagtc agcattgtag gcttcacaga caagtgggtg caagcgaaga
    14341 gccttaccat caatcaagac tggctcgaag gcttggatac ccaaacggtg aagggtcggt
    14401 gcgcggttca aaagcactgg gtgttcttta atcacttctt caaggatatc ccagatacgc
    14461 tcatctccgc gttccaccaa gcgtttagct gctttgacgt tttgcacgat atcacgggca
    14521 acgatttcac gcatgacaaa tggtttaaag agttcaatcg ccatttcacg tggcacacca
    14581 cattggtaca tcttaagagt tggaccaacg gcgataacgg aacgtcctga gaagtcaaca
    14641 cgtttaccga gcaagttttg acggaagcgt ccttgtttac ctttaagcat gtggctcaat
    14701 gatttcaatg gacggctacc tggtcctgtg attggacgac cacgacgacc attgtcaatc
    14761 aaagcgtcaa ctgcttcttg aagcatacgc ttctcatttt gaacgatgat acctggtgca
    14821 tttaactcaa gcaaacgagc caaacggttg ttacggttga taacacggcg gtaaaggtca
    14881 ttcaagtcag atgaggcaaa acggccacca tccaactgca acattggacg aagatctggt
    14941 gggataaccg gaagaatgtt aagaatcatc cattcaggtt tgtttccaga cttgtaaaag
    15001 gcatccaaaa catccaaacg acggatggct ttgacacgtt tttgtccagt agctgttttc
    15061 aattcttctt tgagttcagc aatttctttt tcaagatcta cttgcttcaa aaggtcttgg
    15121 atggcttccg cacccatctt ggcaacaaat gaaccataac catattcacg caagcgctct
    15181 cggtattcgc gctctgtcat gatagacttg tgctcaagtg gtgtatcctt aggatcaatc
    15241 accacataag ccgcaaagta gataacttcc tcgagggcac gagggctcat atcaagggtc
    15301 aagcccatac ggcttggaat ccccttgaag taccagatgt gagatacagg agctttcaat
    15361 tcgatatgtc ccatacgctc acgacgaact ttcgtacgcg ttacttcaac cccacagcgg
    15421 tcacaaacaa ttcctctgta acgaatgcgt ttgtacttac cacaagcaca ttcccagtct
    15481 tttgtaggac caaagatcac ttcatcaaag agtccttcac gttctggttt caaggtacga
    15541 taattgattg tttcaggttt tttgacttct ccataagacc atgaacggac tttacttgga
    15601 gaagctaggg tgatttgcat acttttaaaa cgatttacat caaccactat ttcttccctt
    15661 tctattctaa gtgaactgct tattcttgtt cagcagcttc ttctgttgct tccgcttttg
    15721 ttgctttctc agcttcttca gcttcaaagg ctgctttagc ctcttgagct gctttttcgc
    15781 gggctttttc aaggtcatct acgtggatga catcttcgtc cattccttca tccaagtcgc
    15841 gaagttccac ttcttggtca tcttcgtcta ggacacgcat gtcaagacca agagattgca
    15901 attctttgac aagaactcgg aaggattctg gaacacctgg ttttggaatt ggtttgcctt
    15961 ttgtaatagc ttcataggct ttcaaacgtc cgttgatatc gtccgacttg taagtcaaga
    16021 tttcttgaag gacatttgac gcaccgtagg cttcaagagc ccaaacctcc atctcaccga
    16081 aacgttgtcc accaaactga gctttacctc cgagtggttg ttgggtaaca gttgagtaag
    16141 gtccgactga acgcgcatgc aatttatcgt caaccatgtg gtggagtttg atcatgtaca
    16201 tgactccaac agaaacacgg ttatcaaatg gttcacctgt acgtccatcg taaaggattg
    16261 tcttggcatc gctatccata cctgcttctt taacagttga ccaaagatct tcagaacttg
    16321 ctccatcaaa gactggtgtc gcaatgtgaa taccaagagt acgagctgcc ataccaaggt
    16381 gaagctccat aacctgaccg atattcatac gtgatggcac cccaagtggg ttcaacatga
    16441 tgtcgactgg agttccgtct ggaaggtaag gcatgtcttc tacaggaacg atacgagaga
    16501 caaccccttt gtttccgtga cgtccggcca ttttatctcc gaccttaatc ttacgttttt
    16561 gagcgatgta aacacgaacc aacatgttaa cacctgattg caactcatct ccatttacac
    16621 gtgtaaagat cttaacatca cgaacgacac catcggcacc gtgtggtaca cgaagagaag
    16681 tatcacgcac ttcacgagac ttgtctccaa agatagcgtg caagagacgt tcttcagctg
    16741 aaagatcttt ctcaccctta ggtgttactt tacctacaag aatatcacct tctttaacct
    16801 cagcaccaat acggataatc cccatttcgt caaggtcttt gagggcatct tcaccaacgt
    16861 ttggaatttc gcgagtgatt tcttcaggcc caagctttgt atcgcgcgtt tctgattcgt
    16921 attcttcaag gtgaacagat gtgtagacat cgtccttcac caagcgttcg ctcatgataa
    16981 cggcatcctc gaagttgtaa ccttcccaag tcatgtaggc aacgattggg ttttgtccaa
    17041 gcgccatttc tccattttcc atagaaggtc cgtcagcgat gaaatcgcct ttttcaacga
    17101 catcaccaac ttttacgaga gtgcgttggt tgtaagcagt acctgagttt gaacgacgga
    17161 atttttggat gtggtaaaca tccaatgaac catcttcacg acgaacttct accttgtcag
    17221 catctgcgta agtaacttta ccatcatact gagcaatcac agccgcacca gaatcgtggg
    17281 ctgcttggta ttccatacca gtaccaacgt aaggtgcctg aggattaatc aatggcacag
    17341 cctgacgttg catattggct cccatgaggg cacggttgga gtcatcgttt tccaagaaag
    17401 gaatacatgc tgtcgcaacg gcaactacct gttttggtga aacgtccatg tagtcaacaa
    17461 tattagctgg atactcttgg ttgacccctt ggtgacgtcc catgacaatc ttctcagcaa
    17521 aggttccatc ttcattcaga ctagagttag cctgagctac agtatattca tcttcttcat
    17581 cagctgtcaa ccaaacaatt tcgttcgtga caacacctgt ttcacggtca accttacggt
    17641 atggtgtttg aacaaaacca tatttgttca agtgtccgta agatgacaag ttattgatca
    17701 aaccgatgtt aggtccttca ggtgtctcga ttggacacat acgaccatag tgagtgtagt
    17761 gcacgtcacg tacttcatat ccagcacggt cacgagtcaa accaccaggt cctaaggctg
    17821 acaaacggcg tttgtgagac aactcagaaa gcgggttgtg ttggtccatg aactgtgaca
    17881 actgtgatga accaaagaat tctttaactg cagctgttac aggacggata ttgataattt
    17941 gttgtggtgt caagacttca ttgtcctgaa cagacatacg ttcacggaca ttacgttcca
    18001 tacgagaaag tcccaaacgt acttggttgg caagcaattc accaaccgca cggatacgac
    18061 gatttccaag gtggtcgata tcatctacac ggccaagtcc ttcagccaag ttgaggaagt
    18121 agctcatctc agcaaggata tctgcaggag tcaccgtacg aaccttgtca tctgggttag
    18181 cattaccaat gatcgttacg acgcgatctg gatcagttgg agcaacaacc ttgaattttt
    18241 gaagaacaac aggctcagtc acaacggctg catcgtttgg gatgtagaca atcttgttca
    18301 agtcgccatc caaatggctt tcaatgcttt caatcacgct acgagtcata atcgtaccag
    18361 cttctaccaa gatttctcca gtttcagggt ctaccaatgg ctctgcaatg gtttggttga
    18421 gcaaacgtgt tttaacattg agttttttat tgattttgta acgaccaact gctgccaagt
    18481 catagcgacg tgggtcaaag aagcgagcca caagcaagct acgtgagctt tcagctgtct
    18541 taggctcacc tggacgaagg cgttcgtaaa tttctttcaa ggcttcgtct gtacgagagt
    18601 ccattggatt cttgtggata tctttttcaa cagtgttgcg aaccaattcg ctgtcaccaa
    18661 agatatcaaa gatttcatca tcacctgaga aaccaagagc acgaaccaag gttgtaaatg
    18721 gaatcttacg agtacggtcg atacgagtgt aggtgatatc ttttgagtcg ctttcaagtt
    18781 ccaaccaagc tccacggtta gggataacag ttgaaccata gcccacctta ccatttttgt
    18841 ctactttgtc gttaaagtaa acacctggtg agcggaccaa ctgagaaacg ataatacgtt
    18901 caccaccatt gatgatgaaa gtacccattt ctgtcatgat tgggaaatca ccaaagaaaa
    18961 cttcttgggt cttgatttcg cctgtttctt tattgatcaa gcggaaggtt acaaaaattg
    19021 gtgctgagta gctagcatcg tggatacgag cttcttctag cgtgtatttt ggttccttga
    19081 tttcatatcc aacaaattcc aactccattg tgtctgtgaa gtttgaaatt ggcaatacat
    19141 cttcaaacac ttccttaaga ccgtggtcta ggaaagcttt gaatgagtca gtttgaattt
    19201 caatcaaatt tggtaagtca agaacttctt tgattcttga aaaactacga cgggtacgat
    19261 gtttcccgta ttgaacgtca tgtcctgcca agatgattct cctttgtaaa taagttccaa
    19321 gccttgtcaa tcaggctttt ctaatcgcca tatggttgta aaccccttat caccgtgtcc
    19381 tcttgacgaa ttttcagaat ctttaagcct ctgttacaaa tgctcaaaat cttgaaaaaa
    19441 agcacaaaaa gagcagctaa atctgacttt ttcagaagat ttaactgctg tgagccttgt
    19501 ctggacaata tttcagacaa aacctacgac aaatgattac ccatattata ccctatttag
    19561 ctagattttt caaggggatt cagtaggttt ttggtaattt ttttcccata gaaaacttgg
    19621 catcacattc gaatcacgct atggtacaaa aaactgaaaa aactattgac tgaaaatcat
    19681 tttcaaggta taataataaa cgttaaggcg gtatagccaa gtggtaaggc acggctctgc
    19741 aaaagcttga tcgtcggttc aaatccgtct accgccttct ataacttgat ttatcaggtt
    19801 tcaaatgaac agaaagccca atttgaaggg ctttttttat tttccctcga ataaatacgt
    19861 ataactttaa aaacttttgg agcgagtttg tggcagagtt ctttccatgg cataattccc
    19921 ttttgaaatc aattgaaaaa agcttcgtca cgcctggtga caaaagcctc tagtttattc
    19981 tgtttcttct tctatttcga cttctgtgat ttggactttt accttggtaa cacgtccatt
    20041 cttcacttta tcattggtta agataaactg tttgttttgg ctgaccaatt catagctgag
    20101 tttctcagtc gttggaatgg ttcccactcc tgtcaaataa taaccagcga tagtatcaac
    20161 gtcatcactt tccagttcaa cattaaagta attattgaaa tcattgaggg tcatagttcc
    20221 ttgaacaatg taggtatcct cgccaatctg atgaacatct attgctgctt tgtccgtttc
    20281 atcatcaatt tcaccaacaa tttcctctaa gaggtcttcc aaagtcacca aaccagccat
    20341 accaccgtat tcatccagca agattgccat ttgtctttgg gtatttcgca gttcttttag
    20401 caagtcatcc acaaaaatag tttcaggtac aaaaagtgga tcttgtaaaa ttctcttcca
    20461 aacaatattg tcaaaaccgt ccacaaagcc tgccttaagg agactcttgg tgtgaatgat
    20521 tccaattaca ttgtccttat ccccatcata aaccgggata cgagaataat tttgttttaa
    20581 aatactttgg ataatggctt gactatcatc ctgaatatcc accataaagg catccgttcg
    20641 aggaaccata acctctcgtg ccatcagttc atcgagcgaa aagacacctt gtaacatctc
    20701 aatctcatca gcatccaatg tttcttcact atttgtcagc atataggcaa tttcatcacg
    20761 ggtcatcttt tcatccgcat catcgaatga cataggagtc aaatggctca agaaattggt
    20821 cgaagcagct aaaagccaaa caaaaggact gactagtttt ccgatcccaa tgataaccgg
    20881 cgctgtacga attgccaagg catcctttag attaagagcg attctcttag gatataattc
    20941 cccaaaaacg atggaaatat aggtcaaaaa tgccaaggat agaaaagttg ccacggcttg
    21001 tgctgtttcg ccattcccaa gccaagaggc aatcacacgt cctagagtat cagttaaact
    21061 cgcccctgat aagattgtaa tcagggtgat tcctacctga atggttgata aaaagtggtt
    21121 aggattttct agtaccttca gcaggcggat gtagcgtctg tctccttctt ccgccttttg
    21181 ttcaactcgg gcacgattaa gagaaaccat ggccatttct gtggctgaga aaaaggcatt
    21241 taataccgtc aagataaaca ataaaacaaa ttgtagcaac aaattctgac tacttgggtc
    21301 ttccatagtt cttctcctaa aatagtatct tatcctttat tatatcacaa aatttaatcc
    21361 aatacatatt atttttttct tagcgtaact tccatctctc tcccaaaact ggaagttagt
    21421 ctaagacgga tttccactga tttcacgcgt ttttctcata ctaaaagcta ggttgtggga
    21481 aactggaagt tagttttaga agttactatt ttttcttagt acctgttcta gccctgctta
    21541 aactagtaaa aagaactcct acatccataa gtgacatagg agtttaggct tatcttttac
    21601 tgagttacag ttacttctcc agttcggtaa tccagttgaa gtcccttttg aacattggga
    21661 actagaacat tgaattccaa ttctccatcc gaagacttgg cttcaatctg tgaagctgag
    21721 ggaactaaat cctcgtttga agcgtagtaa agggttacac ggtaacggac acgcttgttt
    21781 tggtccaagg ctttacgcac cttgctttca tagtagtttt gaccagtcga atactcggct
    21841 tgtgcctgat ttgcccaggc tgtctgaaca gcaatgtttt taggattgct tgttgaggca
    21901 tcaaaaccat ccaaaccacc gattaaggca tagcctaaca aatgacctct atcgactgca
    21961 tgggtataag agccctttag attcttgacc tgatgccaac ctggaggagt ccaagaagtt
    22021 gaaccattcc cagtttcttt acgattcttg tactgacgag tggccttaga caagagggca
    22081 ttagctacgg ttggaacagt ttccttaccc actgtctttg ttttattgtc agcgtagggc
    22141 ttacttgaaa ccttggcatc tagatttgtt ttattaccat tgacgataaa agcacctgag
    22201 ccattccact ccagactccc ctttatttga ctcttgactg cgtctgttaa gacactctct
    22261 gccaatgctt gactaggagc ttcaggcgct tgttttttct gactaagctt ggttttagga
    22321 ctattagctg tcgactgcat ctgcttgata taatagctcc ctgtagacaa aagcaataac
    22381 actagcagtc cgattagtgt ctgtcttgtt tttttgttca tatttctcct tatctttaga
    22441 aaaaggctgg ttctccagcc taatttcctg taaatttatg aatcaattcc tgccattttg
    22501 ctggagacag gatagcccat gaatcttgac ccttgcccaa gattccataa cctaccatta
    22561 aaccgattcc tagagccaga gtacctaaaa tcagtactat gatgactaaa agtaaacgct
    22621 tgactacgta gcttgatttc ttattcatct tcatcacttg cctcaatccg ctgaatctta
    22681 gcacctagct gcgccaactt ctcatggaaa ccgtagtaac ctctatccaa gtgaaccaat
    22741 ttaccgacca cagtttctcc ctgtgctacc aaacctgtca aaatcaaagc tgcactggca
    22801 cgaaggtcag ttgaaagaac ttctgctccc tgcaaaggct gtccaccaac aatacgagct
    22861 gtatcacgga taatctcaga atgcaagccc atgcggcgca tctcttctag gtgttggaaa
    22921 cgattttcaa aaactgtctc caccatggtt gattcgcctt ttgcaactgt catcaaggct
    22981 gtaaattgag cctgcatatc tgttggaaat cctgggtggg gcaaggtttt cacatgaaca
    23041 gcttttagat tttccagttg agaacgaaca cgaattcctt cgtcttcttc aattacttca
    23101 acacccattt caagtaactt ggcaatcaag ggacggttgt gctcccagac agcgtctcga
    23161 atcaagacat caccaccagt catggcagca gctaccataa aggttcctgc ttcgatacgg
    23221 tcctggacta cattgtgagt cgtaccatga agtttctcaa caccagtaat ggttatagtc
    23281 tctgtaccag cacctttgac cttggctccc atttcattaa ggagaatggc taagtcaaca
    23341 atctcaggct cacgcgcagc attctcaatc actgtcaccc catcagccag agtcgctgcc
    23401 atcatcaagt tctgcgttgc accaacactt ggaaagtcca tatagatatg agcaccatgc
    23461 aagcgttctg cctttgcttc gatgtaacca gctgtctgac taatcttaac ccccatagct
    23521 tccagacctt tcaaatgaag atcaatagga cggctaccaa tcgtacaacc acctggcatg
    23581 gataccttgg catgacccac acgggcaagg attggcccta atacaacgat ggaggcgcgc
    23641 atcttactga catacttgta aggggcttcc tcagtgatgt cgccagtagc atccaccttg
    23701 acaagatgag cttcctcatc aaagtcaacc ttggcattca aaccaccaac tacctgattc
    23761 ataataaaga catccgacaa aatcggaaca ttctgcaaga cggtctttcc ttcacttgct
    23821 agaatagtcg ctgccaacaa gggtaagact gcattttttg ctccctcgat cgtcacgctt
    23881 cctaccagac gattatcgcc accttgaacc acaattttat ccatactcgt ttcctttact
    23941 cttgatttta taataatcct ctaagcgctt cttgccacaa atgatacagg ctaataaaga
    24001 aagaactcag gatgtagcct atgacaatgc taaaaaaggc tactaataca cgaacttttc
    24061 ttgtattctc agctgtcact tttaaaacct tttcccatct aacaagatcc tttaaaaggt
    24121 aaaaactcac ataaataaag agcatatgac tacttagagt gaataataat tgaaccattt
    24181 gactattata ccatcttctc tgtttgtgcg ctagtttgga aacttcactc aaaaattagg
    24241 gattgttttt caagtaatca atcgcatctt gtagggtttt aacgggcacg attttcatat
    24301 ccgtcttgat tgttttagcc gcttctaggg ctgtttggta gttgtttttc gcgtccggat
    24361 gcgccttttg ttcttcttcg ctaacagggt tatcaggagc aaagaaaata gcagcacctg
    24421 ccctagccga agctacaacc ttcttatcaa tacctccaat gtctcccaca ttaccatcgc
    24481 ggtcaatggt acctgtaccg gcaacaatac gaccattacg aagatctggg tgagctattt
    24541 gagtatagat agctagacta aacatgagac cagcacttgg gccgccaata ccagctgttg
    24601 aaaagctaat tgggacattg ctgattacct ctgtacgatc aatcaagccg attccaatcc
    24661 cattttttcc attttccaag gtgataatct tcccttctgc agacttggtt tgcccatcct
    24721 cttcataggt gaccttgacg gaatccccta atttttgaga actgacgtaa tcaatcaagt
    24781 ctttggaact atcaaaggtc tgatcattga ctgctgtgac tgtatcagag atattgagaa
    24841 tccctttaaa ggttgaatta tccgtcacat tcaaaacata aactccaaag tacttgagtt
    24901 cgatatcctt accagctgtt tttagtcctt gatacttggc catattttgc gatgtttgca
    24961 tgtagaattg attgattcgc ataaattcaa catcggaaga accacctgta gtctcctgag
    25021 cactacgaat atctgtaaaa ggtgtcaacc aagcataaat catatgagct aaagtagcat
    25081 gttgaacacc aaccgtaacg aattgatagg caccagcttc cttatcttct gtgtcattta
    25141 ctttaaggac ttggcgaata tcttccgaac cacctggaac ctctatataa taaggcaagg
    25201 gcactacaaa tgccaagaaa gtcacaatca aggccgcaat gacgtataag ggccatctaa
    25261 tctttttttt catttcttat ttcctccaaa atactctcgg gaacatagca ggcaatatcc
    25321 tgaccaaact tcaaaagctc tctaacgcct gatgaactga tatagagatg ttcaggtcga
    25381 ctatgtaaat aaatagtctc tatatcagaa gacagctgat gattgtagta atcaaaactg
    25441 gcttcatatt gcaaatccga cgcattcctc aaaccacgca ctagacaagt agcacccaat
    25501 ctttttgcaa catcgaccac caattcatca tgagaagcca cgacttcaac attttccaaa
    25561 tgtcccaaag ccttttctag cccccgttta cgattttcaa gagggagaaa tccttgtttg
    25621 tggggattaa aaaaaatacc cacacaaagc ttatcaaaaa gtctgctcgc ccgttcaatg
    25681 atatccagat gcccatttgt catcggatca aatgagcctg tgaataagcc aatcttatct
    25741 gacatagact gtcaccttac taattccata aatcttttcc ttccagatac ccagacaggc
    25801 aatttcttct ggaagttcaa cggtcttatc cgtctcgcac acaaccataa catcttcaga
    25861 aaaaagctct ctctcagcca ttttttcaat atctgctacg atttgttcct tggcataggg
    25921 agggtctaag aaaacgaggt caaattcccc agatacctgt tccaatgccc tttctgcatc
    25981 cattttgagg agttgaaatt ttccaacttc cttggtcatc tggatatttt cagccacgat
    26041 ggtctgagcc ttacggtctc gctccaccaa aacagcactg gacatgccac gcgatactgc
    26101 ttcgatagat aaaccaccac tacctgcata aaggtccaag actcgtccca cttcaaagta
    26161 gggaccaatc atgttaaaaa tggctcccct aaccttatcc gaagtaggtc ttgttgtctt
    26221 gccttctagt gtcttgaggg gacgtccccc atagattcct gatacgattt tcatactgtt
    26281 tattatacca aattatagac aaaaagagaa agaaaaccga accttgcggt tcgattctct
    26341 acaaaatatt ttcgtaagta tcgcggactt cttgaggcca aacacttgtt tgcacttctc
    26401 cgatgtgtct cttgcgaagt aggaacatgg ccatacgaga ttgtccaatt cctccaccga
    26461 ttgtcaatgg gaataggcca ttcaacaaag acttgtgcca ttccaattct aagcggtctt
    26521 catcacctgt aatttccacc tgacgtctaa gagtttcttc atctacacga attcccatag
    26581 aagacaactc aaaggctcca cctaaagact cattccagac aagaatatca ccatttagac
    26641 ccttgtagcc attctcagac tcgcttgtcc agtcatcata gtctggtgca cgtccatcgt
    26701 gcggtttacc atctggcaac tcgccaccga taccaatcaa aaagacggct ccaaattctt
    26761 tacaaatcgc attttcacgt tctttaggtg tcaagtctgg gtagcgttct accaattctt
    26821 ctgtatggat aaaggtgatt tgttttggca agatagactc gatgtcatag cgggcttcaa
    26881 cagctagctc agtcaggcga atagccttat aaatcttctc aactgtttct tttagataaa
    26941 cgatgttacg cttaccattt gggataacct tctcccagtc ccactggtca acataaacag
    27001 agtgggttgc atccaaggaa tcctcatctg gacgaagggc tttcatgtgg acaaagagac
    27061 cctctccttc accaaagcca aaacgagcca aggtgtggcg tttccattta gcaagtgagt
    27121 gcaccacttc ataagtagca tcagggattt ggagaacctt gaccgatact ggattttcca
    27181 caccagacag gttgtcctgc attccgtcac cgaccttact caagatagga ccttgaactt
    27241 cgacaacttc tagcttatct ttcaaatact gggtaaaagt gtttttgaca aaggaaattt
    27301 cttcttgttg atggataaaa cttttcttca tagacaactc ctcaaaaatt taataaacgt
    27361 attatacacc tatttccaag aaaagaaaag agaaaatttt aaaaaaatca gtgtcactca
    27421 aaacactgat ttcatagacc ttttagtcat tgcgaccaaa gatacgtaga atacttagga
    27481 aaagattgat aaaatcaaga tagatgctga gagccattga caccacccag cctgtcgcta
    27541 cacgaccttg tgattgttca taggcgaggc gaatcttttg gttatcccaa gcaatcaacc
    27601 ctgaaaaaac caaaaccatg gctacgctaa tcatataatc aaagaagcca ctagccaaga
    27661 aaatattgac taccatagca atcagtaaac caatgagagc tgccatcatt gctcgaccaa
    27721 tcccactcaa gtctttcttg gtaaaaatac caactgccgc catgacaaag aagagaaggg
    27781 cgctcgatac aaaggcagat aaaactgtac ccggagtata gaaagccacc acaaaactaa
    27841 gggtaaagcc gtttaaaacg gagtaaagta aaaatactgg aagagcagct ggactatttc
    27901 ttgaagccat gctactggca acgaaaacca aagccaactc cgcaaaagta gcaatggtca
    27961 accagagacg cccctgcatc aacaagtaaa ccaactgaga ctgaaagacc gtcaacataa
    28021 ggcctgatac caaagcagat agtccgattc ccagaccaac aaaggcataa accttagcgt
    28081 aaaattgatt gagacctgca cggtcatgta taatagtgtg attcatcctt ttctcctttt
    28141 ctgtgaacat ctttccttga ttataacaaa aaaagttaaa attagcttaa atggaagatt
    28201 aaaataccgg ctgcaactgc aacattcaaa ctctcggcct gccccttcat gctaatatgg
    28261 accaactggt ctgcactttc agccataagg ggactaattc cttgaccctc atttcccata
    28321 actagtacaa aattttctat agaaggcagt tctctgtaat caacagaatc tttagatagg
    28381 gttgttgcta gcactgggat agctaccttt ttagtttcgt caagaagcgc ttgactagtc
    28441 atccggtaaa tgggcagatg gaaatgacta ccttgcatgg aacgtagggt tttgagactg
    28501 tagatgtctg ccgacttatc tgaaacaatc actcctgtaa aacctgctgc atccgcagtt
    28561 cgaatgatag ttcctacatt accaggatct tgcacatctt ccaaaaacaa gaacttgccc
    28621 tgactcaaat cagcttgtcc tacttcttct ttttgaacca cggcaacaat tccctgtgga
    28681 gtctgagaat ccgccaaatc tagcaaaata tcctctgaaa tccagacagt ttgcggaaaa
    28741 gcagctaact gatttcgata attttctagg gcaaagattt tctcaatcgt cactccagct
    28801 tggacagctt cttcaaacaa gtgccagcct tcaatcaaat aggcagactt gcggtatttt
    28861 ttttggtgta atttcttggc attttttacc acagaattgg cttttgaggt tataatagtc
    28921 atagaaatat tataacacaa tcaaaggggt ttggtatgca aaaggttaga atgattgccc
    28981 aaggtagggt gcagggagtc ggctttcgtt ggggtgttta tagcctggcc cttgaaatcg
    29041 gtggcatcac aggtcgggta tggaataacg acgatggcac agtggaaatc ttagcccaag
    29101 cagactcatc tgctatcatg gcaaaattta tccaagaaat ccgaaaagga ccgacacctt
    29161 tttcaaaagt aagctactta gatgtcaaac taagcaactt tcctccctac tctgacttta
    29221 aaatcgcaaa ttaggtctcc agaactattg tatattttgt aaaaaaacag tagaatagaa
    29281 aggtataatt tttaaagaag gaataaaaac agtgaaatct attaaacgtt ttgcattctc
    29341 ggctatggga gtggctatgt tgcttgtctt gactggctgt gtcaatgtcg ataaaaccac
    29401 aggtcagcca acaggattta tttggaatac gatcggagcg cctatggctg aagccatcaa
    29461 gtacttcgct actgataaag gtctaggctt tggtgtcgct atcattatcg taaccattat
    29521 cgtacgcttg attatcttac cacttggtat ctaccaatca tggaaggcaa cgcttcactc
    29581 tgaaaagatg aacgctctca agcatgtcct tgagccacac caaacacgtc tcaaagaagc
    29641 aactactcaa gaagaaaaac tcgaagccca acaagccctc tttgccgctc aaaaagagca
    29701 cggcatcagc atgtttggtg gtgtaggatg tttccctatc ctccttcaaa tgcctttctt
    29761 ctctgctatc tactttgctg cccaacatac tgaaggggtt gctcaagcaa gctacctagg
    29821 cattcctcta ggttctccaa gtatgatttt ggttgcctgt gctggtgtcc tttactatct
    29881 tcaatcgctc ctttcacttc acggagtaga agatgaaatg caaagagaac aaatcaagaa
    29941 aatgatttac atgagcccac tcatgatcgt cgtcttctcc ctcttctcac cagctagtgt
    30001 cacactttac tgggttgtcg gtggtttcat gatgattctc caacagttta tcgtcaacta
    30061 tatcgttcgt ccaaaacttc gcaaaaaagt ccgtgaagaa ctagccaaga acccaccaaa
    30121 agcaagtgct ttctctaaac caagtggacg aaaagacgtt acccctgaac aaccaactgc
    30181 tatcacaagc aagaaaaaac acaaaaatcg caacgctgga aaacaacgtt cgagataaga
    30241 agaaaaaggc ttagctgatt tgctaagtct ttttgcaaat caaaagcccg atgtttccat
    30301 caggcttctt ttctatccat gtacacgttt catataatct tgataacttt cggtatccat
    30361 gagtttttta gcgttcttga cgcgatctgc tgttggtggt ttgactcctt cgagggaata
    30421 tggaattcca agttcacgcc acttgaactc acccatggtg tgataaggta gaatttcaaa
    30481 cttatcaaca tttttgaggg tcttgacgaa cttaccaagt tcaatcaagt catcatctct
    30541 gtctgtcaat cctggaacta gcacgtggcg aatccagaca ggttttccaa tatctgatag
    30601 atactgggca caagccaaga tatttttatt agtttggcta gtgacaatct tgtgctgttc
    30661 ttcgttgatt tccttgatat ccaaaagaac caagtcagtg acagccatga gtttgtcaaa
    30721 cttctcaagg taacgtggtt tattacggaa aggaagagca caggtatcca aggtacagtg
    30781 gattccttgt tccttagcct tggtgaagag agcaatcagg aaatcaatct gcaagagagc
    30841 ttctcctcca ctgactgtaa tcccaccctt atttccccag aaaccacggt agcgcaaggc
    30901 ctctgtcaag acatcatcta ccgtccgttc acgtgactta ttggactcca tagcccaagt
    30961 gtctgggttg tggcaatact ggcaacgcat gtgacagccc tgcaaaaaga caataaagcg
    31021 aataccaggc ccatctactg acccaaagct ttctgtcgaa tgcaccattc ctgtcacttg
    31081 tccataatca attgtttctt cagacatatc gttaccttcc ttgaaaacgt tttatagttt
    31141 tattatatca cgacttgaag gaaaaacaag atttttgcct attttttaga aagcaatctg
    31201 tttttcaatt tcattttcaa tctattatca gattattctt caaaatcgaa gccatataag
    31261 gttgcaaaat agtcctcagg gcgctctgca cggcggattt gacgggcttt accttcttcg
    31321 gtatagagga tttccgcaga acgtaatttg gcattatact ggtagcccat tgaaaatccg
    31381 tgggcacctg tatcatgaat taccagcaaa tcaccgattt ctgtatgagg cagttcgcga
    31441 ttgactgcaa atttatcatt gttttcacag agtgaaccaa ccacatctac cgcttcagct
    31501 ggtccatctg gatgggtcac gttgctaata tgatggtaag ctccgtacat agctggacgc
    31561 atgaggttga ctgctgaggc atccacacct agataggtac ggtaggtttc cttcttatga
    31621 gtcactcttg tgactagagc accgtgaggt gccagcataa aacgacccaa ttcggtgaaa
    31681 atcttgacct gaccaagacc tgctgacgta agaacttctt catacacctt acgaactccc
    31741 tcaccaatca aggcgatatc gttcggctcc tggtctggac gataattaac accaataccg
    31801 ccagaaagat tgataaagtc tagcgaaatg cccaactttt ccttgatttc aacagccagt
    31861 tcaaagagct gacgagccaa ctctggataa tagagatggg tcacggtatt ggacgctagg
    31921 aaggagtgaa tcccaaaagt cttggctcct ttttccttca agatagcaaa ggcttcaaag
    31981 agctggtcct tggtcatgcc aaacttagcc tccccaggat tgtccataat gtctgtcccc
    32041 agttcaaaaa cgcctccagg attataacga caagagatga tttctggaat gcctgctact
    32101 ctctccagat gttcaatatc ttcaaaggca tccaagttaa tggtcgcacc caattcacgt
    32161 gcataggcgt attccttgtc tggcgtgttg ttggaagaga acataatctc agaacccaga
    32221 aagtccagtt tatggctcat caaaagctct acataactag agcagtccac accacaacct
    32281 tcttcttgga gaattttcaa aatagctgga gttggagtag ccttaactgc aaaatattcc
    32341 ttaaagccct tgttccacga aaaagcttgg ttgacggctc ttgccttctc acgaatcccc
    32401 ttctcatcat acaagtgaaa gggagtcggg aactcggcaa caatcgcttc taactcttct
    32461 ctattgataa atggtgtttt cataagcttc cttctattct gtttgcaaag aaaggctggg
    32521 acaatcgttc caacctttag ttctatctct tatttatctt cgtcaaagat attgccaacc
    32581 tcaacatcga tggcttggtt cttgacatca atattggtta ccttcaagag tgacttgtag
    32641 tcaaactgct tttcacgttc ttcttgggca ttgtccgcaa agaccgctac acctgccagt
    32701 tctgagtcga actcgtgcaa gagactaatc ataccattga ccgttccgcc acctttcaag
    32761 aagtcatcca caatcaagac acggctgcct gccttaagac tacgttttga aaggaacatt
    32821 ttctcgatac ggtcaccact tgaacctgaa acatagttga cgctaacagt tgaaccttcg
    32881 gtaattttca ggtcacggcg cacaatgaca aaagagacat tgaggacatt ggcaactgca
    32941 tttgcaagtg gcacaccctt agttgctacg gtcataaccg cgtcaatttt ttggtccata
    33001 aagcttttgg caataatacg accaatattt ttcaagatgg ctggtgtgct aagcaaatca
    33061 gacagataga tataaccacc tggcaagata cggtcacttt ctgacaactt ggtacgcaag
    33121 tcttcaacca tttccttagc atcctgactc gaaatagacg gtgtgaagat gacacctccg
    33181 ccagccccag tcactgtctg gatatgaccg atttcaattt cctcaaaggc gcgtttgata
    33241 atgacgatat cttctgagat ggatgattta gcagactcat acttttcagc aaaagtattg
    33301 agactagtta gtttataagg attattaatc aaatagttgg aaatgacaac catccgatca
    33361 cttcttctta atttcatttc tatttcctat tccattttta ttcaaaaaat caaaaagcaa
    33421 actagaaagc tggtcgctgg tggttcaaaa cactgttttg agattgtcaa tagaactgac
    33481 aaaccctgta atatacctgc atatatacat acgacaaggc gatactaccc tagtttgaag
    33541 agattttcga agagtattca tttttgtctt ttacttatta taccatattc acataaaaaa
    33601 acgaacattc ttatcctaaa aaatgctcat ttttcttaaa ttatcaatct aaatctggtt
    33661 tatagaagga acgattatcc atagcgaaga ttttattggt catctctcct ttatccacca
    33721 aagccagagc tgttgacatc atcatcatgc ttgcatccag attgtcaatc atatggataa
    33781 tctctgcctc cataatacgt ggacggactg gacttccata ttcaagcaag ccgtggtgac
    33841 tgaggatgac atgacgaagc aaaacgactt cttccttggt atcatcgatg ccgagttcca
    33901 taactgtctt ggtaatttcg ctatcaatga gagcgatatg tccaagaaga ttacctcgca
    33961 ctgtgtactc tgtctggtct ggccccgtca actcgataac cttagctaag tcatgcagca
    34021 taatccccgc atagagcagg ctcttattga gctgaggata aacttcgcta atagcgtctg
    34081 ccaaacgtac catggtcgcc gtatgataag ccaaccccgt ttcaaaggca tggtggttgg
    34141 tcttggcagc tggataggag tagaattcct tatcatactt ggtgtagaga tttcggacaa
    34201 tccgttgcca gacaggattt tcaattttga aaatcatttg cgacatgtag tcacgaattt
    34261 ccttgacatc aactggtgac ttgaccttga aatcggctgg gtcattgggt tcaccagctt
    34321 gaggcaggcg gagagtaatt tgattgactt gaggggtatt gttataaact tctcggcgtc
    34381 ctttcatgtg gacaacctta cctgcggtaa aggcctcaat gttatgaggt tgggcatccc
    34441 agagcttccc atcaatctcg ccactatcat cttggaaggt aaaggctagg tagtttttcc
    34501 cagctcgagt ttgcctcagg tcagctgatt tgattaggta aaagccttca aatagctcat
    34561 cttttttcat gtgactaatc ttcatattct tcctcatttt cttgaaaatg gagtagatca
    34621 agcgcaggct caccttctga caactcaatg tgacggagcg tccgctcgat agctatggta
    34681 cgacggttta ataattcatc aatattgcca gaggcatgtt ggagatgttt ttgtgccttg
    34741 accagaatac caccaaactt gccaaactcg gtcttgacac tggcaagagt cttgctgata
    34801 tggtcggcac tcttttggat attaagggtc ttgaaaccaa ctgatagaga gttaagaagg
    34861 gctgataggg tacttggtcc tgcaacaata atctgttctt cccgtctcaa atcatcaaag
    34921 aagaccggat tgcgcacaat ctctgaataa agaccttctg tcggaacaaa caagattcca
    34981 aagttggttg tccgaggtgg tgctatgtac ttgttcctaa tatccctagc aaagcgcttg
    35041 acgcttgcta ggagtgactt acgacagcgt tcaatctcat ccttgtcacc tgtctcatag
    35101 gcttcttcca agcggtaata atctgccagt ggaaacttag agtcaattgg cagatagacg
    35161 tattcttggt cgccttgtcc gggtaacttg atggcatact ccactcgttc actagagttt
    35221 tcaaccgttg cgtattctcg ttcgtactgg gcaggtgtca tgatgtcttc aataatttgc
    35281 cccagttgca attctcccag aatccctcgc gtcttggttc cagagagaac cttgttaaga
    35341 gctccgacat cacgggcaac tgtctgcatt tctccaaggc cacgattgac agactccagt
    35401 tgtttagaaa ctgtctcaaa ggaagcctgt aagcgtgtct gcaaggtctt ttctagtttt
    35461 tcctcgaccg tctggcgcat ttgttccaaa cgttgctcat ttgattcctg caaggcttgg
    35521 agacgttggt ctgtcttgtc tctagtttgg aggagattat ctgtcatttc ttgacggact
    35581 tgggtcagac cttggtgcaa ttcaatccgc acttcttgca aacggtcgct gacaaccact
    35641 tccaaatctt tttggtctaa ctggctggct tgtctggctt ggtcaaagcg gtaatccaac
    35701 tggtctgaca aatgatctgc ctgatcctcc aagctcttac ttaagtgttt ctcctgccta
    35761 tcctgccttt gccaaatcag aaagagacca gctagattgg caattaataa tagaataagt
    35821 aaactttcca tcctacctcc tgtccttgct atgcagtacg accacataac catctgggca
    35881 agttaccgac acttccctat ctatatattc gttagaagcg tacacttttt taaagaaaaa
    35941 attttcctct gtcaactcat acttggctcc aagaatagtc agctggctat cccgaactgg
    36001 cataaaggct agatagtcgt agtctgaacg aggttctagc tgactgattc cttctggaca
    36061 ataagtaatc aagttttgcc catcctcaat ttctatttga tgcatatagg atgccaactt
    36121 aggattgcta ggcagaaaga cattggccaa catatggtca atacggccac ccaaggcacc
    36181 gaaaatagta acctgagcct gaggattttg cttaaagatg gtcaagagag ccaattccaa
    36241 atcagtatca tctttttctg gccgggcttg gacaaaatac tgggcacgtt tttgaatcac
    36301 ctgtcgctct tcttccgtca cagaatcaaa atctccgact gctagagcaa gaggcaaatc
    36361 ttcttccaag atccagagcg agcctcgatc cacaccaaca aaaccatcaa agtccgtccg
    36421 ataatgaccg cggtctccgc ctgcaaaaac ggcaacccta gtccagttgt tttctgagag
    36481 tttgtactcg ctcattgaca tctcccttaa agacatagga acctgctaca aaaacggtcg
    36541 caccagcttc tttggcttga gcaatagttt ggtcatcaat cccaccatcc acttcaatct
    36601 caaagttcaa acctttttca tcacgaaggg caaccagctc acgaacctta tccatggttt
    36661 ctggcagaaa ggcttgacca ccaaatccag ggttaactgt catgactagg acttggtcaa
    36721 ctagatgaag cacatgctta atcgcttcaa ctggtgtacc aggattgata acaactgatg
    36781 gcttgacacc gagtgaacga atcttttgga gggcaccatg aatatgaggc gttgcttcca
    36841 catggatact aataatatcc gcacctgcac gcacaaagtc ctctaaatga tgctcagggt
    36901 ttgacaccat caagtggcag tcgaaaacca tcttactatg aggacgaaga gcttcgacca
    36961 cacccgcacc aaaactgatt tgcggcacaa aatgaccatc cataatatcg atatgggcat
    37021 actctgcccc agttgcttct aggcgtttaa tttcacgttc aaagttggca taatctgctg
    37081 ccagaattga cggagcaatc ttgtattgag acataggttt ctccttattt tggaattttt
    37141 ttgctgactt ttttataggt ttctctgcga ttttcaattt cgctgaggaa ttgcaggtag
    37201 ttgtcaaaac ggaagggcgc aatggtacct tcttcaactg ctggcttgac agcacaggac
    37261 ggctcatggg tatgggtaca agtacggaat ttgcagtctc gactgacagt ggcaatctct
    37321 ggaaaggcct gattgaggtc ttcagccgtt gatacttcat aatccagtga tgaaaagcct
    37381 ggtgtgtctg cgattttacc cccattgagg ttgtaaaaac taacagctcg agtggtatga
    37441 cgcccgcgac ccagactgtc tgaaatttct cccgtttcaa gattaagatc tggtgcgatt
    37501 ttattgagaa gagttgattt cccaacaccc gtctgcccca taaagaccgt aaccttgcct
    37561 gttaacaaag gcaggagttc ctctttactg gtcacaaagt catatccgat gtcaccatag
    37621 gtctgctggt aaaaatccag ttctcccccg tcttccaaca aatccatttt agaaatatag
    37681 acgatgggat gaataccctt atgctccaaa agaaccaaga aacgatccag caaattgctg
    37741 ttaaaatcag gttccttgac agacatgatt actacagctt ggtcgatatt gacaataggc
    37801 ggacggacca gactgttttt ccgttcgtga atcttgagaa tatagccttc tgaattttcc
    37861 tcggcagaga aatctaccca gtccccaacg taaggggtat gccctttttt acggaaattc
    37921 ccacgcgcgc gtgtttgata gacttggcca tcactctcta catagtagaa cccagccaag
    37981 gctttaatga tttgtccctg caaaataact cctttaaaat tgttagttcc attataacaa
    38041 aaaaaagcta ttttagctta gcttctcttt acaatttcga taagtctatt ctaaaagaaa
    38101 aagatacact cacgctttag ataccaagtt ttatacaaaa ataacgaatc tacctcctta
    38161 tataaaaaca ggcgaacaga gagcctaaaa ctccctgttc gctttacttt ttctgtgtgt
    38221 aatagaaaaa acctaataaa gcaagacaaa aaatatgaat ataaactcca tacatcatgt
    38281 ggggaaagac agactctata aaatagcctg gtagatagat gattggaaat tgatagaaat
    38341 cataggtcgt tagagatagt aaacaaagaa catacataaa tactgctaca attagaaccc
    38401 atccttgctt tcgccataga aaaatcatca ataagaacag acaaagtaga tagattgacc
    38461 aatttcgcaa cataaattga aaaccataaa tcatactttc agtcgtttct ccatcaatca
    38521 catcaactac tttatagaaa ggtaaacaat agctcactaa aaaaagaagg ggactaatta
    38581 tttttttcat attctctacc atggattatt ttgcaagtaa gctgaccatc tatctccata
    38641 tcgaatcaaa ttaacagtat aattagttac tacccgattt tcacctggtc caaccttaga
    38701 aaaacctgtg ctatactccc aactacttct tgcacctgga tgaaaccatt tttgctggga
    38761 tgaataccaa ccaccttgtc taacttccac acttgaagat tctatttcga tatccctttc
    38821 atggaagtca actcctccac ttattttagt aatatttttt tgtacacctc catcataact
    38881 cacccagtga acaacagtat acaattccac actataagta ctatcaaact gactttgatg
    38941 agtagtaatg ttttgagtat aagcgtgcac tttctgtata ccaaaaatat ggtctaatat
    39001 attcgttggt ttagcaagct tattgtaacc ctcttcaaca aaagtagcct tctctaaatc
    39061 agcggtataa acagtttttc cttgtactac ttgattgttt atagaagtaa tacggctatc
    39121 cacaacaatg ggggccacca tatctccatc gctagtttct attttagctg cctctttcac
    39181 ctcatataca actcgatttt cttcagactg aatcacttct accccatttt tttcaggtat
    39241 agataatcta gtttgcccat tagaaaccgt tatatttgga ccaatattct tctcatttgc
    39301 ataagtcgca ataggaaatc ctgatagaac cactgcacac ataaggctat tgactaattt
    39361 ttttctcatg aatatatcct tcccattttc aagaggagta aaaatagcta atataagatg
    39421 aacgtttcct tatatttcag aataggatat ctatctgaaa gcaattcaca ctcctataga
    39481 taactgttcc aaatcttggc tacatatatt tcaatagttt gctacaaaaa actcctttct
    39541 aagtctattg aacatgccct tattcaccta ctaatatatc acaaaaataa ataaatacaa
    39601 gactgttttt tctactagtt ttatgataga atagtattgt tagggtaaat ttcatatata
    39661 aatgtcgagt gaaaaatcga tggagcagaa atcggtagac aaagactcga tttcgttggt
    39721 ccagccccac aaggctaatc aggtatttca cgagtttaaa aatctaatga agtgcccaat
    39781 caactactga ttcattgatt taatacgaac tatagtggat tgaacctaga aatagtacac
    39841 taaggattct aaaacgtttc tagaaattaa tttgactttc ctaatctctt tgttcatatc
    39901 ttatttcaat ctactatata taccaaaatg ctaggacaaa agtctttaaa atcagagaaa
    39961 cgcatattat caggtgttgg aaaaccttga taatatgcgt tttatcatag aaagatctac
    40021 ttcatttttt actaaaatta agtttttgtc cctgcatctt agagtccttg ccctttaagt
    40081 gcatctgcta ggctggcaaa ctcttccaag ctgagagctt ccccacgcac acttggtgac
    40141 aagcctgctt ggtccaaagc cttggtcagc ttgtccttga cctcttcagt cttgccaaag
    40201 taacctgtca agttattcca caaggtcttg cggcgatggg taaaactagc cttggaaacc
    40261 ttaaagaaaa agttctcgtc ttccactgct acagctggca ctggacggcg caccattttc
    40321 aagatggctg agtctacatt tggtgctggc acaaagactg tacgcggcac gataaaggca
    40381 accttggctg tcatgtaata ctgcacagcg atagacaagc taccgtaagc cttggtattt
    40441 ggttgagccg agatacggtc cgccacttct ttttgcatca tgaccacaaa ctcactaaaa
    40501 ggaatgccac tctcgattaa gtgcatgaga ataggcgttg tgatgtagta aggcaaatta
    40561 gccactacct tgattggcag gtcaggattt ttgaaattct ggatatgttg cgccaaatcg
    40621 accttgagaa tgtcctcgtt gactacagtc acattgtcaa aatcacgcaa ggtatctgcc
    40681 aaaatcggta ccaaacgatg gtcgatctca aaggccataa cttctgctgc acgctcagcc
    40741 aaaaactcgg tcaaggcacc aatacctggc ccgatttcaa tgacattgac ctggtcatca
    40801 atctcagccg tatccacgat tttttgtagg atattggtat ccgtcaagaa attttgccca
    40861 aaggactttt taaaggtaaa accgtgacgc tccagcactg ccttggtcac gctataatct
    40921 gcaattctca tctattctct tttctattat ctgttactac tattgtaaca cattcccctt
    40981 gtattttggg ggtgaaaatc ataccaaaaa actatctttc ctcatgaaga aagatagtct
    41041 tggaaatctt taaaagtggc cttagcgctt ctctcgaaac atacgttcat actcttcgaa
    41101 aatctcttca aaccacgtca gcttcacctt gccgtactca agtacagcct gcggctagct
    41161 tcctagtttg ctctttgatt ttcattgagt atcaaaaaaa tcagcttctt gataacttcg
    41221 attttcgggt agggtcaaat ccacatcctc ctccacatca tccactgtca gttcaaccgt
    41281 ttgaggtaaa tttttcaaac gataatgctt ttgcctttgc ccttcactac tagcaaaatc
    41341 aagcaaatag ccatgcactc ggatttgaac caaaaggaga ggattggtca tattttccat
    41401 gatgaggata tcttataaac cacctctggc actaggataa tcagataccc tgcgaatacg
    41461 gattttttca cacatgagca agtttcctca tatcaatgat ttcatcagcc ttattccaga
    41521 ctaggggatt atgggtcgca atgatgataa ttcgattttc atctttcaaa tccaccaaga
    41581 gattcataac ctcctctgaa ttttcaggat caagagctgc tgttggttca tctgccaaaa
    41641 tcaagggtgg atttttcaaa atagttttcg caagggcaac tcgttgggcc tctcccccag
    41701 ataaagtata aattttttgt tctaaatcca aataccctag attaactttt tctaaagccc
    41761 ccacttgcct ttccaaacgt tctacttttg agattttctg accaacaaaa cccaaatcca
    41821 aattttcttt gattgattgg ttttctaaga ggccaaaatt ttgaaagaga tagcccatct
    41881 gatctcgaaa atactcacga gtgggaatgg tttttaaatc tttcccctga tagagaaccc
    41941 ttcctccatc tatcttttct agctttccca agatattcag cagcgtcgtc tttccgcttc
    42001 cactctttcc gattaaggca taaaccttgc ccttctccag cttgagattc aaaccagaga
    42061 aaatcgcgcg gtcattaaat ttcttttcca atccttgaat atcaatcatc ttattttcct
    42121 ttcaatactg ccatgctacc agcttcttct tttttgtcct gccttactaa aatcaagagg
    42181 gcgttaagcg taaacaaagc tacaactaga gcgctaatca aaccatctct tgtcaaaata
    42241 ctagatagga ctaggccaag caagagaact cctccttgcg ccagtaaata cttgccatga
    42301 agctcataga ttgtcatacc agcaagacgt ttaatcataa gctcccgtct gaactgctca
    42361 aaatagagaa gattcatgga atcaaataac aagatagccg tagacagggt caaaatcgtc
    42421 ccaataagga gagagtaaaa ctctacccgt ttgtcattta gtaccttggc aaaaaatagc
    42481 tggcttttta ccaagtaaga aaccttgtga tacagacctt gctcttttag agctgtgatg
    42541 ctgtctccat atcgatctag tttcaaggca ctattagccg tagttcccca caacatccct
    42601 gcaacaggtc ttgttccagt cgcttgcggc gttagaacta caataatcgg atcttttagg
    42661 tactggcgtg ttgtcttgta cccatcatta tagaggaaac gttcctgtcc tgtttctgta
    42721 aaagctagac ttacctgtgg gaggtagtat ttctgactcg tcacttctac actttcagat
    42781 gaaaagtttt gcaggtaatc tgtaaacaat ctttggtagt aggtagactg ctctcgcaag
    42841 ctctcaggca agattagccc aaactctccc tcagacaagt tttgcatctt gtccataaac
    42901 tctgaagaaa cggtaatctt ttcttctatc agatagcgtg gtgagacata gataacattc
    42961 cctgacggtg tgtagtcact gagacgattg ccatctaggt ccacttctgc tccgtctgag
    43021 aaattgtcaa cattgctcat aatataaaaa gagtctgtat tggctaaccg ttcttcagta
    43081 aatgtctgcc actcacgatt atccttacgc gttccttctt catcggcaaa tgcactagac
    43141 caaccaaagg atagacggta acggtctgag gactggctcc atttattgct agctctctcc
    43201 atttcctgca tttcacggta gtggggtagg agagctgtcg cactcgatcc gaccaccaat
    43261 acagctaaga gttgccccac catcatcaat gtcatcatac gtttgagagg gagtttccct
    43321 ttcaatagat ccaccagact attttcctgt aaaccaagta gatagacgac acttagtaag
    43381 gtagaaatcc ctgccagggt caatccataa agtagaagag caatgatgac cagttgcacc
    43441 gttgccataa acaaggcacc ttgataccag agaatcccca atcccaaaag actggatacc
    43501 agcactgagc agataaactg tctcacatct tctaacactg gtctgagagc aactccaaac
    43561 aagctctcac cagctattaa gcgaatccct gcctgacgaa gggatttgat ccgataaatc
    43621 agggtcagac tcataaaggt cagcagaaaa atagctaaac tcagtagcac cataggggtg
    43681 gccgccaata gtaagactat cgaaaatgga tcttctccat tcgaaacaaa gccttgatag
    43741 ccaagttctt tcaaggtggt ctgtaagctc actccatcca aacttcctga cacaatcaag
    43801 taagacccta ataaatcact cgtttctgca ctctccttgg aggaaatggt cagaccttct
    43861 ggaagctctc cctcaccata agtggcatag gtaaagagtg tttctccagc ttcatttggc
    43921 tcaacgattc gacgggcgat gagactatta tgctctaccg ccaagtgact caaaacctct
    43981 gtcacctctt cgcgactagc tactggttga tttatcatca ttcctgtcac atcaacagaa
    44041 ggatagttac caaataaaat ctcttctcga tggtcaacgg aagttatcac catataaagc
    44101 gataccaata ccattgaaat caaaataaat aaacgtttca tcatttcctc caaaaagtta
    44161 cttggtcact tagtgagaag gtgaccacag tcactgcgat gttaagaagc ttattaagtt
    44221 ctttctacta gcaacatctt caaatacatt atatccgttt tttcaagaac tatcaaggac
    44281 atcaatgtcc ttaaaatcta actatttcat attattttta gcttatatct aaaaaaacag
    44341 ttctaaagac gaactttaga actgttttga tacgaggaca aggacatttt tgtcactgat
    44401 tcactatgtt tataaatatt ttttgacatc ctcttgccat tcctctgtca atttctttac
    44461 tagaaatcca tctccaatta gcttcgcaaa tgttttagaa gactgataaa atgattcagc
    44521 taaactaaaa tctctttctt ctcttaatgc ttgcttccac aaaaacataa atacaagagg
    44581 tttcttttga taatcttgag ttttatccat aatttcttgt aaacacgata gataatcatt
    44641 aaaacaatca gttactccta ccttttcaag acaacacatt cctgaaataa gagtatctct
    44701 aattataaag aagttttcta atttagttac ctttttctgt tccaaaagaa agagcattag
    44761 cttttcgatt tgatttataa atttggcaag atcttttcta atgagcattt gataaaaaaa
    44821 caaacgaatc aacaacatat catttgttga ataaacttct ttttctatga tctgagacat
    44881 atgttcctca agtattaact ctgcatattc aggccttcta gttctagaag tatccaaaca
    44941 cgcctgcaat acttcacatg ctaattgttc ttctttagga agattatcaa agtatgtttt
    45001 ataaatctct tccaaacacg cttccttctt atcgtactcc tcttctttac catagattgg
    45061 ttcacgtaaa atctggtatt tcaattctaa ataagcagga ggaagagctg aaaaatccgg
    45121 cataaggcta taaactggaa ctcctagccg gcgagcaata taggctaact tgggcaaact
    45181 cggttgcgaa gctccacttt caattcgcga cagttgacga actgtcagtt cttgctcatc
    45241 tccacaaaaa tatggacgac taactccttt ttctatccga atctctttaa ttttttgacc
    45301 aattaacatc agacgcctcc tcttctacct gcttcgaaaa atactgtgaa acttaaaaaa
    45361 ttgtaacggc tacatctttc ctactcataa gatttcatag cttcttccac ttctgccaaa
    45421 gtaaccccaa acaactctag gcgtttgagg agttgcttgc cgttagaata gccgattcgg
    45481 agagtctctc cgagatattc tctacgctta cggctgtctg cccctgctag aaaaccaagg
    45541 cgaatcaagt cgctacgact aatgtcaaac tgactctcat gttcaaattg ttctgtcacc
    45601 tgagctagag ccgttttcag gtcttcatag ctggcatgct caattcccag agaacgcccc
    45661 ttggtcttgg actttggaac agcttcatct cgcttgagaa aggcatgccg aactgttgga
    45721 atggccgtca taatcatgcg ccgaatccgc tccccattaa aatctgggtc tgtaaagaca
    45781 atgactccct gacgttggtg caggcgctga atccgctcta tatcctgagc attgatggca
    45841 gaacctcgag tctcataggt ctccacatcg aaataacgtt tgagattgac cgtatcatca
    45901 cgcccttcaa ccacgataac ttgagaaatt ttctctttca ttacttgctg tccaatccaa
    45961 aaatgcgttc tgcatttgca gtcgttgcta ccgccagctc ttctgtcgtc ataccacgca
    46021 agtcagcgat aaagtcgacc acatagcgag tataggctgt tttattttca cgaccacgct
    46081 tgggtacagg tgctaagtaa ggcgcatctg tttccaccaa aatcttgtcc aaaggtaact
    46141 ctttagctgc ttcttggagg tcagttacct tcttaaaagt caccactcct gagaaggaaa
    46201 tggtcatacc aagatccaca aacttctctg cccactcaag cgtccctgaa aatgaatgca
    46261 tgataccacc acgaggacca acgccctcac tcttgataat ctcataggta tcttccagcg
    46321 catcacgggt atggacaaca aaaggcaaat ccaagtcctt agatagctga atctgacggc
    46381 gaaaaacctg ctcctgcacc tctttgggcg ctgtcatcca atggtaatct aagccaattt
    46441 cacctaaagc cacaaccttg ggatgtttta acttatccaa caagtaagcc tcaacttcct
    46501 ctgtataagt accagcttct gtaggatgcc aaccaatagt cgcatagagc tgatcatact
    46561 catctaccaa ctccaaggca cgctcaatcg tcggtttatc aaaaccaaca atattcatct
    46621 gtgtcacacc catctcagca gccaaggcaa tttcttctgc ctcacgacct gcaaattctt
    46681 ctacattcaa gtgtgtatgt gtatcaaaaa tcatctcttc taacctcgtt ttctatcttc
    46741 tattatacca aaaaagagga ggggcaccta atttttcggt ttcccctcct ctcttcaata
    46801 gagagctatt ctgctatctt ttctatccga tattgcccat ctcctattcc acagttagag
    46861 acagaagaga ttggctacat tgatagaata ttcctattca gctaaagtat gtgattatct
    46921 tgggttctca cttttctcac ccatcactag gattgatgac ttctcaaaat cccccttata
    46981 atctaacttt aagtagagaa tagaattttc tagctgttct attttctacg tctagtcttt
    47041 cttcctagca aagcaaaacc acttgttgcc accactagac caagactagc aagtgctgca
    47101 cttgatgctg aacctgtgtt aggtaattgt ttgccagcag gtgactggta ggtaacatct
    47161 tgcattgttc ctgcagttgc ttgcggtgca cttgttgtct taggagcagc attaggagcc
    47221 actggtctct taggaactgt aaccatgaca atggcttgca ctccaatcgt ttctgcttcc
    47281 ttcacatctt ccatagtttt cgcattttca atctctttga gagctgcttg tttttctgct
    47341 tccactcttg ctaaaagttc tgctttttct ttatcagaaa gcggtgcgcc tttgatttca
    47401 tcctgcttgt ccttggctgc tttttcaatg gcatttttag ctgattcttt ttcagtagct
    47461 aattgcttag aagcttgtga ttgagctgag ttttgtgaac ctgtgtgagc tgatccaccc
    47521 gcctggctac caccgcctgc attcgcatta gcatctgagc ttccagtatt gcctccctta
    47581 tctccacctg gtgtattttc tagcttatac tctggtgtag cttcttgagt tacagattca
    47641 cctttcgcag attgttgaac tgtatctttt cctagaatcg tcactactga accatctggg
    47701 aatgtgattg ttgctgtacc atctccagct acattgattg tcgctccgtc taatgctgaa
    47761 ccatttgctt gtaaaatagc aaccttaact ttttcttcat ctgttaaatg agttatatct
    47821 tttacatcta ctctttgagc aggtgcttta acagttgatt tttcagcagt cggtttagtg
    47881 ctatcttgtg cttttggatc ttgtgctacc aattgatttc ctggaattgt aatagctgaa
    47941 ccatctttaa agacgattgt cgctgcgcca tctccagcta cttcaacttt atagtaaaat
    48001 gaaataataa caggacaaat cgatcaggac agtcaaatcg atttctaaca atgttttaga
    48061 aatagaggtg tactattcta gtttcaatct actataacat cgcttccgaa tccatttgca
    48121 tctactgaac ttttttacaa aaataaaaat cagtagactc gagttcctac tgatttttga
    48181 tagaattcgt ttggattatg cagccaaaac tttgcgtcct ttacgacgac gagctgccaa
    48241 tacgcgacga ccatttttag ttgacatacg gttacggaat ccgtgtttgc gcgcacgacg
    48301 aagtttactt ggttgataag tacgtttcac gatgaatacc tcctcataga ttttgtattc
    48361 gtttagccgg ctataaagtg atcagttact aaacatactt tactattcta tctgattctt
    48421 tcacttttgt caatagctct aggcaaatgt ttccagcttt ttcgaatgca agcc
//
LOCUS       DAVBBS010000008        45604 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVBBS010000008 DAVBBS010000000
VERSION     DAVBBS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45604
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.008"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(76..525)
                     /locus_tag="VQU32_000448"
     CDS             complement(76..525)
                     /locus_tag="VQU32_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET2471964.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(527..1285)
                     /locus_tag="VQU32_000449"
     CDS             complement(527..1285)
                     /locus_tag="VQU32_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET2471965.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(1400..3379)
                     /gene="pknB"
                     /locus_tag="VQU32_000450"
     CDS             complement(1400..3379)
                     /gene="pknB"
                     /locus_tag="VQU32_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HET2471966.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(3376..4116)
                     /locus_tag="VQU32_000451"
     CDS             complement(3376..4116)
                     /locus_tag="VQU32_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET2471967.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVFMNEEDAE"
     gene            complement(4131..5444)
                     /gene="rsmB"
                     /locus_tag="VQU32_000452"
     CDS             complement(4131..5444)
                     /gene="rsmB"
                     /locus_tag="VQU32_000452"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HET2471968.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(5437..6372)
                     /locus_tag="VQU32_000453"
     CDS             complement(5437..6372)
                     /locus_tag="VQU32_000453"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET2471969.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(6385..8781)
                     /locus_tag="VQU32_000454"
     CDS             complement(6385..8781)
                     /locus_tag="VQU32_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET2471970.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGTVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(8847..9161)
                     /locus_tag="VQU32_000455"
     CDS             complement(8847..9161)
                     /locus_tag="VQU32_000455"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET2471971.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(9186..9812)
                     /gene="gmk"
                     /locus_tag="VQU32_000456"
     CDS             complement(9186..9812)
                     /gene="gmk"
                     /locus_tag="VQU32_000456"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET2471972.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(9942..11555)
                     /locus_tag="VQU32_000457"
     CDS             complement(9942..11555)
                     /locus_tag="VQU32_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET2471973.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(11746..12000)
                     /locus_tag="VQU32_000458"
                     /pseudo
     CDS             complement(11746..12000)
                     /locus_tag="VQU32_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            complement(12004..12258)
                     /locus_tag="VQU32_000459"
     CDS             complement(12004..12258)
                     /locus_tag="VQU32_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HET2471974.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            complement(12496..13593)
                     /locus_tag="VQU32_000460"
     CDS             complement(12496..13593)
                     /locus_tag="VQU32_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HET2471975.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVLD
                     YQKIADLYTEKGAEMEKFVKNLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQARENVLPEAIHVLGFTEKVRQHLKSLKGQVNLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
     gene            complement(13603..14343)
                     /locus_tag="VQU32_000461"
     CDS             complement(13603..14343)
                     /locus_tag="VQU32_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2471976.1"
                     /translation="MATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLELA
                     CGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYD
                     FVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHENA
                     EDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQAG
                     FKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(14663..15016)
                     /gene="rsfS"
                     /locus_tag="VQU32_000462"
     CDS             complement(14663..15016)
                     /gene="rsfS"
                     /locus_tag="VQU32_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET2471977.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(15029..15529)
                     /locus_tag="VQU32_000463"
     CDS             complement(15029..15529)
                     /locus_tag="VQU32_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET2471978.1"
                     /translation="MKTAFIIIDVQNILVETGFQTKSLLGKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(15530..16123)
                     /gene="yqeK"
                     /locus_tag="VQU32_000464"
     CDS             complement(15530..16123)
                     /gene="yqeK"
                     /locus_tag="VQU32_000464"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HET2471979.1"
                     /translation="MSYQDYINCSREALLEKMAEFLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSNQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIANLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            complement(16123..16752)
                     /locus_tag="VQU32_000465"
     CDS             complement(16123..16752)
                     /locus_tag="VQU32_000465"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET2471980.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(16804..17115)
                     /gene="yhbY"
                     /locus_tag="VQU32_000466"
     CDS             complement(16804..17115)
                     /gene="yhbY"
                     /locus_tag="VQU32_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HET2471981.1"
                     /translation="MSLASKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(17329..18435)
                     /gene="yqeH"
                     /locus_tag="VQU32_000467"
     CDS             complement(17329..18435)
                     /gene="yqeH"
                     /locus_tag="VQU32_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HET2471982.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVATRKAII"
     gene            complement(18438..19028)
                     /locus_tag="VQU32_000468"
     CDS             complement(18438..19028)
                     /locus_tag="VQU32_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HET2471983.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(18980..19888)
                     /locus_tag="VQU32_000469"
     CDS             complement(18980..19888)
                     /locus_tag="VQU32_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET2471984.1"
                     /translation="MVLEKQLGHGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(19945..20505)
                     /locus_tag="VQU32_000470"
     CDS             complement(19945..20505)
                     /locus_tag="VQU32_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HET2471985.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            20644..21853
                     /gene="sstT"
                     /locus_tag="VQU32_000471"
                     /pseudo
     CDS             20644..21853
                     /gene="sstT"
                     /locus_tag="VQU32_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            21921..22874
                     /locus_tag="VQU32_000472"
     CDS             21921..22874
                     /locus_tag="VQU32_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET2471986.1"
                     /translation="MSISQRTTKLILATCLACLFAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            complement(23110..23190)
                     /locus_tag="VQU32_000473"
                     /pseudo
     CDS             complement(23110..23190)
                     /locus_tag="VQU32_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(23245..23469)
                     /locus_tag="VQU32_000474"
     CDS             complement(23245..23469)
                     /locus_tag="VQU32_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HET2471987.1"
                     /translation="MIEMLIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFVLLLTFMVITYK"
     gene            complement(23474..23653)
                     /gene="asp4"
                     /locus_tag="VQU32_000475"
     CDS             complement(23474..23653)
                     /gene="asp4"
                     /locus_tag="VQU32_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HET2471988.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            complement(23655..24992)
                     /gene="gtfB"
                     /locus_tag="VQU32_000476"
     CDS             complement(23655..24992)
                     /gene="gtfB"
                     /locus_tag="VQU32_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HET2471989.1"
                     /translation="MIELYDSYSQESRDLHESLVATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYVDGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGACFAKTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHHPDKSGSDVLVW
                     QEPLYDAIPGNMQLILESDNVRTKKIIIPNKATYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIVEALPDVTFRIAAVTEMSSKLLDMLRYPNVVLY
                     QNASPQKIQELYQLSDIYLDINHSNELLQAVRQAFEHNLLILGFNQTVHNRLYIAPEH
                     LFESSEVAALVETIKLALSDVDQMRQALGKQGQHANYVDLVRYQEIMQTVLGG"
     gene            complement(24985..26496)
                     /gene="gtfA"
                     /locus_tag="VQU32_000477"
     CDS             complement(24985..26496)
                     /gene="gtfA"
                     /locus_tag="VQU32_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HET2471990.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     ADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDENKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEAQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFI
                     VSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLREIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            complement(26508..28880)
                     /gene="secA2"
                     /locus_tag="VQU32_000478"
     CDS             complement(26508..28880)
                     /gene="secA2"
                     /locus_tag="VQU32_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HET2471991.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEDPKKEMK
                     AEEKKLIYASDIIYTTNSSLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIIDTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAIRAHKLFTKDKDYIIRGNEMVLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMTGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKQYHAKGNPLLVFVGSVEMSQLYSSLLF
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDIVYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWVEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIRADMVRNLLMGLVEVTPKGEIVTHFP"
     gene            complement(28873..29313)
                     /gene="asp3"
                     /locus_tag="VQU32_000479"
     CDS             complement(28873..29313)
                     /gene="asp3"
                     /locus_tag="VQU32_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HET2471992.1"
                     /translation="MIITQRQSIYWGEVGGTYMYGTTVSYYLDKSVRLYNPLLPSGEI
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     KVEKAKDFIFTYPEDSYTYQVSLLSAGFESLTFYHFSIKEIRSV"
     gene            complement(29310..30845)
                     /gene="asp2"
                     /locus_tag="VQU32_000480"
     CDS             complement(29310..30845)
                     /gene="asp2"
                     /locus_tag="VQU32_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HET2471993.1"
                     /translation="MSKELNILQIGLANWENHYDIPENMSWYYFYPNSSKTLREIIEK
                     EDINRFHAVLIEDGQYSRDLFSYVKYFEPYTLFYNQNLQINDREVVDFLKKRCAQAID
                     FLSPQQLINDLSKSLFGGGYGDKLFPPTIQVNPNFTGAISYQGLDYVSLEGEFGQDFA
                     QLAYWAYNIMVQKTLPIELWLEYEKEGNCDFRLVIRKMWSGSVDDFFEEVIVSEKDLE
                     QALFMDSRDGDYFLSISVEARGRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEI
                     NYFFHPGDFKPPLTVYFAGYRPAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDE
                     LEGKVKDTITHYLDYLGFDHKDLILSGLSMGTFPALYYGASFEPHAIIVGKPLANLGT
                     IASRGRLDAPGVSNLAFDCLIHHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYM
                     KDEEMDPQAYEQLVSYLCNTGAKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGR
                     EKR"
     gene            complement(30829..32409)
                     /gene="asp1"
                     /locus_tag="VQU32_000481"
     CDS             complement(30829..32409)
                     /gene="asp1"
                     /locus_tag="VQU32_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HET2471994.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLETDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFEPNGQIHMHHIMDDRGFISSIIF
                     FEDGQAAYQEYLNPKGVWQFRERLKEGGQVEVNPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHSHHDQLVLDRLPSTNPKLLSLFIGRNPQDTFRDLDVTFEKS
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYFQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNEVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRLQFVNLNDELDLIKTLEFVRLIVDLNRHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLTDVTEFSKAAHYYTDRLKEWNESLIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            complement(32419..33636)
                     /gene="secY2"
                     /locus_tag="VQU32_000482"
     CDS             complement(32419..33636)
                     /gene="secY2"
                     /locus_tag="VQU32_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HET2471995.1"
                     /translation="MTKIYSSIAVKKGLFTLFLLFIYVLGSRITLPFVDLNTKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSFSKRLGLTSTSIEIQDRR
                     KMYLTLMIAVIQSLAVSLRLPVQSSYSAILVVLMNTILLIAGTFFLVWLSDLNASMGI
                     GGSIVILLSSMVLNIPQDVLETFQTVHIPTGIIVLLALLTLVFSYLLALMYRARYLVP
                     VNKIGLHNRFKRYFYLEIMLNPAGGMPYMYVMSFLSVPAYLFILLGFIFPNHSGLAAL
                     SKEFMIGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSR
                     FINRLVLRFSVIGGLFNVVMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNETYKPLI"
     gene            complement(33693..34370)
                     /locus_tag="VQU32_000483"
     CDS             complement(33693..34370)
                     /locus_tag="VQU32_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET2471996.1"
                     /translation="MGISVAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNY
                     YNYDESDGNFYFYVTGQDFCVEELAIQEIMNRQAGDWKFNSSAFILPTFKLIKKELFN
                     EVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVE
                     VFSKKISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDA
                     EQRNGKS"
     gene            complement(34355..34597)
                     /locus_tag="VQU32_000484"
     CDS             complement(34355..34597)
                     /locus_tag="VQU32_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2471997.1"
                     /translation="MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDGSTD
                     NSGEICDAFMMQDNRVRVLHQENKGGQHKLKIWGLV"
     gene            complement(34597..35793)
                     /locus_tag="VQU32_000485"
     CDS             complement(34597..35793)
                     /locus_tag="VQU32_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HET2471998.1"
                     /translation="MRKSIVLAADNAYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKMEVVNSTIRSVHIDKELFESYKTGPHINYASYFRFFATEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNAGMLLMDVAKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLALDKTYNYMVGVDIYHLAQECERLDDNPPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMLVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELTSLSQYTNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFAFDITRKSMDDGLYDGIFSVERPDDLVDRM
                     KNIEIE"
     gene            complement(35802..37022)
                     /locus_tag="VQU32_000486"
     CDS             complement(35802..37022)
                     /locus_tag="VQU32_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HET2471999.1"
                     /translation="MRNTKRAVAFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKV
                     LYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
                     KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIP
                     LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIF
                     KLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPM
                     LLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSS
                     IPKKMVAAIRSYMR"
     gene            complement(37053..39497)
                     /locus_tag="VQU32_000487"
     CDS             complement(37053..39497)
                     /locus_tag="VQU32_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HET2472000.1"
                     /translation="MIKTIVLAGDRNYIRQLETTIKSILYHNRDVKIYILNQDIMPDW
                     FRKPRKIARMLGSEIIDVKLPEQTVFQDWEKQDHISSITYTRYFIADYIQEDKVLYLD
                     SDLIVNTSLEKLFSICLEEKSLAAVKDTDGITFNAGVLLINNKKWRQEKLKERLIEQS
                     IVTMKEVEEGRFEHFNGDQTIFNQVLQDDWLELDKEFNLQVGHDVTAFYNKWENYFNE
                     LVPPSIIHFVSYRKPWTTLIANRYRDLWWEFHDLEWTKILQHHIGEFELTSSLDKEFS
                     CLTLTNSQDLEGIEELVTALPDVVFHIAAWTDMGDKLIKLAVYDNVRLHPQIVPPVLD
                     KLKKSTNLYLDINHGSADENFLKSLQEQEKTLLAFQSTQHGELGQIVFENGKVSFMID
                     TIKDFKKNGHLTCFRQLPSLTCLTFTASQDIEQLDYLAGQLPNVVFQVAAWTAMGPKL
                     YDLSNRYPNIQLYPAISRDKLDELKEKMDAYLDINLLTSTSDIVAEMAHLSKPILAFY
                     KSQNGNNGQRLYSSEHPERMLADLQKLITKDMLEKPLDIIQVKGIDETLDYIIEHNSS
                     LVRFGDGEINMLAGHSIPYQDYDEELVSIMRDIIGQESREDLVVCLPDAFTDRFRFTS
                     WAIPFWKDHMDHYMDFYRELCSDSWYGSTFVSRPYIDFEDKSQAKAQFEKLKSIWENR
                     DLLIVEGATSRSGVGNDLFDEANSIKRIICPSHSAFSRVHEIEQEIEKYAGGRLILCM
                     LGPTAKVLSYNLCQMGYQVLDVGHIDSEYEWMKMGAKTKVKFSHKHTAEYNFDQDIEF
                     IDDETYNSQIVARILN"
     gene            complement(39507..40517)
                     /locus_tag="VQU32_000488"
     CDS             complement(39507..40517)
                     /locus_tag="VQU32_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET2472001.1"
                     /translation="MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIA
                     SDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDV
                     VPLMFDSNYYLMKDYMYMYNLSDVLIVPSERMKTRLMEEGLTTKKILVQGMWDHPHDL
                     SLYTPAFKKELFFAGSLERFPDLQNWSQDTPLRVFSNKGEASSSARNLSIEGWKKDEE
                     LLLELSKGGFGLVWGTYQNDGESNQYYTLNISHKVSTYLTAGIPVIVPSSLSTAKFIV
                     DQGLGFVANSLEEVHAIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLG
                     VE"
     gene            complement(40546..41136)
                     /locus_tag="VQU32_000489"
     CDS             complement(40546..41136)
                     /locus_tag="VQU32_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HET2472002.1"
                     /translation="MEHDALDRLYGALKKENADISIGRYNSYDETRYVYMTYVTDPDD
                     SLEVIEGKAIMDREGVEEVRNGNWTVAVLKLFKRELLQDLPFPIGKIAEDTYWTWKVL
                     LRASRIVYLNRCVYWYRVGLSDTLSNTWSEKRMYDEIGAREEKIAILASSDYDLTNHI
                     LIYKNRLQRVIAKLEEQNMQFTEIYRRMMEKLSLLP"
     gene            complement(41144..41422)
                     /locus_tag="VQU32_000490"
     CDS             complement(41144..41422)
                     /locus_tag="VQU32_000490"
                     /inference="COORDINATES: protein motif:HMM:NF021616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2472003.1"
                     /translation="MENELISIVVPIYNVENYLRQCLDSIQKQTYPNFECIMVNDGSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGGVHYFCRL"
     gene            complement(41462..42340)
                     /locus_tag="VQU32_000491"
     CDS             complement(41462..42340)
                     /locus_tag="VQU32_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HET2472004.1"
                     /translation="MKKNHLVGDALILTVSDQIEELDYLLESLPNICFYIAAPVQFSE
                     KIRSLETNYNVRLRTITNEEQLNFLVDTCDFLLDINHFQEVDAIVSKFVQAGKSVFAF
                     DNTVHGNQGQEVFLSSTPDKLVSRVRDYLNEVRVGTNHQEKIIQDGTWNVFKIDDKAH
                     FIVGANVICRNFENFHVSSGKLILHDGVFINNSCSFNCMERIEIGAGTMMGEGVRFYD
                     HDHIYTAEKIEKWQWTTAPIRVGRDCWIGSNVTILKGVTIGDNTIIGAGCLIRNDIPS
                     NSVVYNNGNLVVKRRD"
     gene            complement(42337..44422)
                     /locus_tag="VQU32_000492"
                     /pseudo
     CDS             complement(42337..44422)
                     /locus_tag="VQU32_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000341103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(44483..>45604)
                     /locus_tag="VQU32_000493"
     CDS             complement(44483..>45604)
                     /locus_tag="VQU32_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2472005.1"
                     /translation="STSASASASTSASESASTSASTSASASASTSASASASTSASTST
                     STSASTSASTSASTSASTSASTSASESASTSASTSASTSASASASISASESASTSASA
                     SASTSASASASTSASESASTSASASASTSTSASESASTSASASASTSASASASTSASA
                     SASTSASESASTSTSASESASTSASASASTSASASASTSASASASTSASASASTSASA
                     SASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASA
                     STSASASASTSASESASTSASESASTSASASASTSVSNSANHSNSQVGNASGSTGKSQ
                     KELPNTGTESSIGSVLLGVLAAVTGIGLVAKRRKRDEEE"
BASE COUNT        13298 a        10244 c         8171 g        13891 t
ORIGIN      
        1 tattctcgca catatagaaa gcgttgttaa tttatttatc tcatttttca gaaaattctt
       61 ttatttctgt aaagtctacg atactcgatg tgtttttata taatgataca gtctgagaat
      121 cactgttccg ctagccattc caatagagat taccaaagcc aacatgacaa ccaaggtcgc
      181 acttgccagt gctttattat agtcccctgt cacaaaaaag gcagttgttc ggtaggagag
      241 ataacctgga accagcggtg ccaaaatggc caagataaag accacagcag gtgtcttata
      301 aagaatactt aaaatctggc tgacacaaga accaataatg gctgcaatga aggtagctac
      361 aatgacattg gtcggttcct tgagcaagag atagattagc cagacagtca tgcccaaaat
      421 ccctccaggt aagagcatag accgttgcac attgagtacg attaaaaaag tgataatggc
      481 aagaaaactt gctactgctt gtaataaaaa ggttgttagt gtcatattag ttcatcaata
      541 ccaaggcgac agaagttcct gcccctaaag cgagggtaat gagcagggat tcaaacatct
      601 tactcatacc agagtttatg tggttggtca taatatcacg gaccgcattg gtcaaggcaa
      661 tacctggtac aaacggcatg accgcaccag ctataatcaa atctgccgtt gaaggaaaac
      721 ctgtgtagcg agcccaaaac tgggcaatta tcccaaagac aaaggctcca gcaaaggctg
      781 tcacaaaggg aattcggata aatttttcca catagaggga aaaggcaaaa ccaaataagg
      841 tcgccactcc tgccccaagt gcgtcgtaga tatttccgct aaacataact gaaaagaaag
      901 gagcactaaa ggtcgcagcc agagttacct gcaacttagt atagggaagg ggttgagctt
      961 gcaaggccgt caattgctta aaggctgttt ctaagtcaat ctgcccccca actagctgac
     1021 gagaaatctg gttcacatcg cagacttttt cgatgttata agaagaggag gtcacgcgct
     1081 tcatgcgcga aatattggta ttttcaatag agaaaaagat agcggcaggc atggcaagga
     1141 cattgcaatc cacaatcccc tgcgaatgcg cgattcgaat catggtatct tctacacgat
     1201 ggatttctga gccactttta aggagaatag tccccgctag cataatcaca tcaatgacgg
     1261 catttaattc ttttgattct tccatgcttt cctcctttta tcaactccct ctattctatc
     1321 acaaatccgg actcaaaaaa aatctttgcc atgaaatcat gacaaagatt gattactcat
     1381 tttgattatc catctgcttt taaggagtag ctgaagttgt tttaggtttg tagattgaaa
     1441 tcttgactct agtcttattg aggtctacct tttcacctgc tctaggactt tgttcaacaa
     1501 ccatgccttc tacactacct gcaggcgctg tcgtcacttc tacaacttct atattagctt
     1561 ccttaatccc aacaatttga atcaaattgt tcttagtaaa ctccaagctg gaaccaatgt
     1621 aactcggcat ggcaacactt gtaacttttt tagctactgt caagacaatt tgagtaggtt
     1681 tactcacatc ataagtcgtt ccggcacctg gactttgttt cataatcgtt cctggttcgc
     1741 tttcgctgga ctcttcttcc tctatcttaa tcaaattctc aggaaccttc ttctgcttga
     1801 gttctgagat tacttctgta gagttccgtc caatatagtt ccctaattga atcgtcgtag
     1861 cttttttagc tactgtcaaa acaatttgag ttgccttgct caagtcatag gtcgtacctt
     1921 ctggtagact ttgcttcagg accgttccag cctcactctc attcgactct tcttcctcaa
     1981 ttttaatcaa attatctgga acttttttct cttttaattc cgcgataaca tcagaagatt
     2041 tccggccgac ataattacta atttggaagg attgtttgcc tgatgagaca accagattga
     2101 ttttcgttcc ttcttttcga ccagttccag cgccaggatc tgtacggata atccgccctt
     2161 cttccacctt ttcactagcc tctgtcttct cctcaccaat ctcaaaattg gcttttttga
     2221 gcgttgcctt ggcctctgca actgtctgac ctgccacatc tggaatggca atggttgcag
     2281 gagttctgga tagtatccaa ataagagaag ctgccaccaa tacaaggctg gccaacaaaa
     2341 tcaggtaacg catcttaaat ctatgttttt tcggtgcttg tggttggtaa gtttcctctg
     2401 tcacagcctg gcttgggttt ttgattgatt tgtgttctgt ctgcgcttga accttaggaa
     2461 tagatgtcaa ggtactctga gaaaccttcg gcaaggtctt ggtatctgcc ttgctcgttt
     2521 catcaaagat taacttactt tcatttctac gattgtagga caagctacta gacaagtcca
     2581 catacatctc tgaaaccgag cggtagcgat tggtcaactt tttagcagtt gccttgataa
     2641 taacattttc taaagcctga ggtacagatg gattttctgc aataacggac ggcaggggtt
     2701 tctggaaatg ctggagggca atggtcaccg cgctatcccc gtcataaggg atatggcctg
     2761 tcaacatctc atagaaaata atccccatgg catagatatc actctgcaca gtcgccttcg
     2821 aaccacgcgc ctgctctggt gacaagtaat gaactgagcc caacatcgag ttagtctggg
     2881 tcagacttgt ctctgcaaag gctacagcaa tcccaaagtc tgtgaccttg gcagtcccat
     2941 ctggtgtcaa aaggatattt tgaggtttca agtccctgtg aacaattcct cgagtatggg
     3001 ccaagcgcat agccaagaga atttgtccca tgatacggac tgcttcttca ttagaaagag
     3061 gataatgttc cttgatatag cgtttgaggt ccagtccagc cacatactcc atagctaggt
     3121 actgttgacc gtcttcctcg ccaatatctg ttatccgaac gatatgagga tggtctagat
     3181 ctgccatagc tctcgcttca cgctgaaaac gagctacagc tatcgggtcc gtctggtagt
     3241 tggtcctcag aaccttcact gccacttctt ccccatctaa gattaagtct ttggctaggt
     3301 agacatccgc catgcctcct cgaccaatct gtttgacaat ccgatagcgt ccggcaaaaa
     3361 tcttgccgat ttggatcatt ctgcatcctc ctcgttcata aaaacaaggg caaccgtaat
     3421 gttgtctaaa cctcctgcat tgttagcaaa acgaacaagt gtctccgttt tatctgctaa
     3481 aggaatatca ctggttacaa tatcacgaat ctcactgcct gaaatcatgt tggtcaagcc
     3541 gtcactattg agcaagagat agtcacctga ctcaaggata actgtcccaa aatcaggctg
     3601 aatttcatct ttttgcccaa tagactgggt gataatattt ttttgcggat gagcttctgc
     3661 ctcttctggt gtcaattgac cagccttgag caattcatta accaaggaat gatcgctcgt
     3721 caactgatgg tattcttctc cacgaatcaa gccgatacgc gaatcaccaa tatgagcata
     3781 gatagcctga ttatcaataa tagcaaggac ttccaaagta gttcccatgc ctctgtaagc
     3841 ttcatcctga ccaagctggt gaatcttttg attttcaatt tctaggtaat gggcgaacca
     3901 ttcacgcact tcattgactg tatcgatctg ggtatcaacc caagctacac ccaggtctgt
     3961 gaccgccatt tcactagcga tattccctgc acgatgacct cccatcccat cagctaaaat
     4021 aatcatggta cgtccagctc tattgacata gtggttgaca tagtcttggt tatttgttcg
     4081 tttctgacca acatctgtta ataatgaaat ttccatgtgt cagttccttc ctaatccgat
     4141 atcttgcgaa attgactgat gaagaatcca tcacttccat acaattcagg tgtaatgagg
     4201 atacagccgt ctttcatgat atccttacat tcatgttcta gttttacctg ctcgaactcg
     4261 ggatgactct ctaaaaaggc cttaacgact tgaaaattct cctctgagac gatagtgcag
     4321 gtgctataag ttattatacc acctttgcct agtatttgac aaacactacc taatatttct
     4381 aactgaattt cctgcaagga cgcgaaatct gccgtttctt tattgtattt gatatctggt
     4441 tttcggcgca aaagaccgat tcctgaacaa ggagcatcca ccaaaatctt atcaaaggaa
     4501 tcctggtcaa aaaactcatg cacctttctg gcatccaatt tttgagtttg aacccgatct
     4561 tcaactccca gacgttgggc attttcttga attaaatcca acttgtggtc gtacaagtcc
     4621 agagcagtaa cctgacctgt cgtaagataa gaggctatat gggctgtttt cccacctgga
     4681 gccgcacagg catcaagcac tcgctcatca ccttgtaaat caagcgtcgg agcaaccaac
     4741 tgactggact cgtcttggat ggtaatggct ccatccgcaa acaaattatg ccctgcaaaa
     4801 tgcccctgct ccttaaccag accagtggtt gctaaaaggg aattattcgc ctccaacaag
     4861 gcttggattt cctcttttcg acttaggtct gttacacgaa tactggcttt gtttcgcact
     4921 aacaggcttt caaagatggc ttttgctctc tcctctccgt attcttcctt gagtttggca
     4981 actagccaaa ctgggagaga ataggcaatg gagtcacgct tgttttttcg cttgatgcta
     5041 gcaatatctg gccagccttc acgcaagata cggcgaagga cagcgttgac caatttttca
     5101 ctgccttttt tacggagttt ggccaattcc actgcttcat tgaccacagc atgatctgga
     5161 atcttgtcca aatagcggag ttggtaggca ctcatgagaa gaaggacata gagccagctg
     5221 tctaactggt ctctgtcttc gataaagtgg gataggtacc attccagagt cagtttacgg
     5281 gctaccgttc catagactag ctcggtcact aagcccttgt ctgctgcaga gagctgactc
     5341 cccttgaggt gtttatttaa ggcaatattt gaatatgctt gattcacaaa aacatcctct
     5401 agcactgcta gagctaaact tctagccgtt tctactttag tcaccaaatc gttctcctac
     5461 agtcaatgta cgtccaactc cattgaggaa ggaagcaatg tccatcttag gcttaccagc
     5521 tggctgcact tgtttgaggg atagagcccc ttcagccgtt gcgacaatca attctttctt
     5581 gccgatagag agaatctcac ctggatttcc ctgaccttct actggtaggg cttcataaat
     5641 cttaaagcgg tcgcccttaa ggaaagtatg ggcaacaggc caggggttca ttccacgaat
     5701 ttggttaaag agttgacgat tggttttgtt ccagtccagt ttttcttcct ctggctttat
     5761 atttggagag aaggtaacct gactcgtatc ctgcggttca ggtttgatat caccagcaat
     5821 ataggcaggc agagtgtcca aaagcaaatc acgaccaact agcgccaatt tttcaaacaa
     5881 ggtgccaaca ttgtcctcat ctgtgatcgg aatgctgcga cgagaaatca tatctcctgc
     5941 atccatttcc ttaaccattt ccatgatggt cacaccagct tcctcatccc cttgaatcaa
     6001 ggcataatgg ataggcgcac caccacggtg tctaggaagg agggaggcat gaacgttgac
     6061 agcaaagtcc atgctatcaa ggagtttgct tgggagaaac tgcccaaaag cagcagtcac
     6121 aattccatct gctcctagct tcataagttc ttccatctct ggacttccag ataatttttc
     6181 aggttggtag atagatagtc ctgcttcctt ggcagcctgc ttgactgggg tttcttggat
     6241 aactttttta cgaccaacag cacggtctgg ctgggtcaca acggctagaa tttcgtaacg
     6301 gtcatctgtc aaaagtcctt ttaagactgt tgctgaaaag tcgggggtcc ccataaagat
     6361 tagttttgtc atatcttctc cttcttataa aaattgctgc ggctcatggt caatgctgag
     6421 acggagctca ctattttccc gttcttgagt caaggccaag acctggttga gggtcggccc
     6481 cagctcatct tctaaacggt atttaattaa aatctggtaa tgatagaggt tgtgggtacg
     6541 ggcaatcggt tttggcgttg gccccagaat gggactggtc tctgacaagc ctgaccgcaa
     6601 aatgttcatg acttcatagg cacgtttgaa aacctcttct tctttcttgt gagaaagggt
     6661 aataccaatc gtgaaatagt aaggtggata gccgagttgt cgtctgattc ccatttcata
     6721 ggcataaaag ccttcgtaat cttgatcctt ggcaaatcga atagcatagt gctgcgaatt
     6781 gtaggactgt atcaagactt gacctgcttt ttcagcccga cctgctcgac ctgccacctg
     6841 agtcaagagc tggaaggttc tctcagaaga acggaaatca ggcagattca aggccgtatc
     6901 cgcatttaga actccgacta gggtaacatt gggaaaatcc aaaccctttg caatcatctg
     6961 agtaccaagt aaaatatccg cttcccctcg cccaaactgg tcaagcaagg cttggtgact
     7021 gcctttcttt cgagtcgtat ccacatccat cctcaaaatg cgagcttggg gaaagagttc
     7081 tgctagctca tcataagcct tctgagttcc cgtcccatag taacgaatac tgcggctctt
     7141 acagttagga cagacctgag gaatatcctt cgagaaacca caataatggc agttcatagt
     7201 cttggtatcc atatgcaagg tcagagaaat atcgcagttg ggacaagtat ccaccgtccc
     7261 acactcccga cacatgacaa agctagaata accacggcga ttgagcatga gaaccacctg
     7321 ctctttttta accagacggt cttggatagc ctctagcaaa ggaggcgtaa agtttgacgt
     7381 ctcattttgt ccgatatagt ctcgaaagtc aatcacttga acctcaggga ttgtagccaa
     7441 aggattggca cgttgggtta gacgtaagtg ttgatagacg cctttgccag cacgtgcccg
     7501 gctctctaag ctcggcgttg cagatccaag taccagagtt gcttgattat actgagcccg
     7561 taaaatagct acctctctgg catggtaacg gggattgctg tcctgcttat aagccgcttc
     7621 atgctcttca tcaataatca tgacacccag atttttcaga ggagcaaaga tagcagatct
     7681 ggcaccaaca acaacttggg catcgccacg ctccaccttg cgccattcat catacttttc
     7741 gccattggat aatcctgagt gaagaatggc taccttgtcc ccaaaacgtg ctataaaacg
     7801 ctcggtcatc tgaggagtca aggaaatctc aggtaccagc aaaatagctg tcttaccctt
     7861 atccagggca ccttggataa tctgcaagta aacctcggtc ttcccacttc ctgtaatccc
     7921 ttgaagtaga aagggaggtt gagaactgcc aatagaactc acaaccgcat cacgcgcctg
     7981 tctttgttct ggatttaact ccaaaggtct acttgcttca attccttcaa aataagcagc
     8041 cgagcgttga acttcctttt ggactatggt aacagtacct tgatccacaa agaagttgac
     8101 ttgctctcgc gagtaggact ctaacaagct agccaaggaa gcgctctctg gatgagacag
     8161 cagataatct ctcagttcca actttttctt ggcacgtgta gaaatctcaa caccttctaa
     8221 ttgggcatgg tcaacctcat accaagactg ggtcttgacc ttcttttgat cgactgcctg
     8281 atattccaga ccaagcaggc cttttctagt caaacgcatc atttcagctt gcttggcaag
     8341 gtctagtgaa gaaaaggcta gcgaatcttc tgaaccaaac aggcgtactc gttcttcctg
     8401 actcaagcct tccagaggat agagaatctt gtcatagcta gaattcagaa accctggaag
     8461 catggccttg aggatagaga ttttgtagga gaagacagat ttgcgtaact cctcagccag
     8521 ccagagttgt tctggcgtga gaacaggaga aaaatccagc acctctgcaa tatcttttaa
     8581 atcttgctcc atctcttctc catctgattg ggacttcaaa ccaagaacaa tcccttgaat
     8641 caggcgatta cccttaccaa aaggcacatg aacccgcatc ccaacttcca gcattccctc
     8701 aaattcctcc ggaatcctgt aactataggg ctggtccgtc tgcatcaagg gcacatctac
     8761 gataatctta gctagggcca tcttctcacc tcctccttgt cagtacattc ttgcaataga
     8821 aaaaataaga ttgagtcccc ccaaccttaa attttttcac catcttcttt ttctttagca
     8881 atttgctctt tgattttctt ttcttcttct tctttgcggc gtttttcttc ttcgatacgg
     8941 cgacgcactg cttcacgttt tccttctgga tctgggtgaa ttgtaacgtt tcctgattcg
     9001 atttcttcta aagcgcgaag agttgatttt tcagacttga aaccttgagt tgctggggca
     9061 cctgcttcca attcgtgggc acgttttgct tccaagatta cgagtgaata ttttgaagga
     9121 accttgtcga gcaaggtatc aatagagggt tttaacatca tttgcttgta cctattttct
     9181 aaattttatc gggtagttgg agattttggt aacatctcct gatagtgacc aatgacacga
     9241 tccacacaga agtgttctgc ttcaatcaca catttgacac gttcagcagc taggggcacc
     9301 tgatcgttga caatcgcata atcatactca cgcatgaggg caatttcttc cttggccttt
     9361 tcgattcgtt gggcaatcac ttctgcacta tctgttccac gacctaccaa gcgatcttgc
     9421 aattcatcca aatctggtgg tgtcaggaag ataaagacag catctggaac ctttttcttg
     9481 acctgaagag caccctgaac ttcaatttca aggaaaacat cgattccctt gtccaaggtt
     9541 tcattgacat aggtcagagg agttccatag tagttgccga catattctgc gtattccaac
     9601 atctgtcctt gacgaatcag ctcttcaaat tcttcacgag tacggaagaa atagtcaaca
     9661 ccgtccactt ctccaggacg ttgtgcgcgt gtcgtcatcg atacagaata ttgaaattgg
     9721 ttttcagaac tctcaaaaat ctctcttcta accgttcctt ttccaacccc tgaaggacca
     9781 gaaaaaacga ttagtaagcc tcggtctgcc attgtgtctc cttttagtca atctgtgaaa
     9841 taacatttct ctagaataat ggcaaaaagc cagattatcc tttacagtct ttctatctag
     9901 tgtaacaaaa aagcagtaat ttttcaactg ctctttctta tttatttagc ataatctact
     9961 gcacgaagct cgcgaatcac ggttaccttg atatttcctg gataatcgag attgttttca
    10021 attttcttac gaactttgtg agccaagatt gtgactttgt cgtccttgat ttttcctgga
    10081 ttgaccatga tacgaatttc acgtcctgct tgaagggcaa agctagtttg cactccttca
    10141 aagccgttag caatttcttc caaatcatgg agacgcttga tgtagctttc aagagactca
    10201 ctacgagcac ctggacgggc tgcgctcaag gcatctgctg cagcgacgat aactgctatc
    10261 acgctctcag cttcaacatc tccgtggtga ctagcaatcg tattcaccac aactgggggt
    10321 tccttgtact tacgggccaa ttccataccg atttcaacgt ggctaccttc aacctcatgg
    10381 tcaatggctt tcccgatatc gtgaaggaat ccagcacgac gggcaagagc cgcattttca
    10441 ccaagttcgc tcgccatgat accagccaac ttagcaacct caatcgaatg gcgcaaaaca
    10501 ttttgtccat atgaagtacg gaactgcaaa cgtcccataa tcttcatcaa gtctggatga
    10561 aggtttggcg caccaatttc ataggcagca gcctcaccgt attcacgaat cttattgtca
    10621 atctcttgac ggtttttctc aaccaactct tcgatacgag ctggatgtat acgaccatct
    10681 ttgagcaaca tttccatagt catacgggca atctcacgac gaatcggatc aaatcctgac
    10741 aaggtcacca cttctggtgt atcgtcgata atcacatcga cccctgtcaa actttcaaag
    10801 gtacgaatgt tacgaccttc acgaccaata atgcgtccct tcatagtatc gtctggcaga
    10861 tgaactgttg agtttgttga ctccgctaca tattcaccag cgatacgttg catagcttga
    10921 accaagatgt ccttggccat tttgtcagaa cgttccttga cctcttgctc agcttcgcga
    10981 atgcgactgg caatctccct ggtcaagttt tcctctgtct gagccaagat aatatctcgt
    11041 gcttctgcct gagacagagc accaatacgc tctagttctg cttctttttg tctttcgact
    11101 tcctctaatt gctcttcacg cgcatcaagg tttttcgctc tatcagaaat actttgttct
    11161 ttttgttcaa gtgtttgttc tttactcgtc aaattgtcgt ccttacggtc aaggctagta
    11221 gctctctctg tcaaacgact ttcgatttgt ttgagttctt gacgttctga tttgaattca
    11281 gcgtccactt cttcacggta ttttctggct tcttctttgg cctccaatag tgcttctttt
    11341 ttaagagact tgctttcacg tttggcttca ttaacaagta aatccgcttc acgctcagct
    11401 tgtccacgta aattagttgc ttcttgttca gcatttaaaa gcatcaactc tgcagcttcc
    11461 tgagatgatt tcatcttagc tgagatgctg acatatccaa tgactaaacc aatgatgacg
    11521 gcaaaaacag caatcgcaag cgacatgatt tccatgtttt tacctcattt tattgttatt
    11581 ccgaatgaca tacattcttt tacattctac cataaaaaag tgattttcac aaacctaaaa
    11641 tagaatatgt tttggggaat ttggaacaca tttaccaaaa taaacttgtt gtttagaaat
    11701 agtagtttag tagagacttg agaaaaagcc tactttgcag tagacttagt aatgatcttt
    11761 aaaggacaag aaagccacgc tatctccatc catcatataa atcaagcgat tttctgcatc
    11821 aatacgccgt gaccaggctc cttggtaatc atatttgagt ggctctggtt ttcctattcc
    11881 tgtaaatgga tcgcgttgaa tatccttgat taatttattg attcttttta gtgttttttt
    11941 atcttggttt tgtcagtagc aataatctgc ccaggcatct tctgtaaact tgagtagcat
    12001 tcatcactcc tcaatcacat ggacctgagt gcttccagca cggacttgag ccattcctcg
    12061 caaaaccttg tctgataact ccttattttg ggcgattctc agggtttcct gaatactgtc
    12121 ccattcgctc tttgaaagaa ctacaatgtc ctcatctgga tttttattga ccaccgtcaa
    12181 aggctcaaat tcatcattta ccttcttcat gtagtccttt aaatgatttc ggaatgttga
    12241 gtaaaggact gcttccataa ccatacctcg ttttagctct tttccactat tatacacgaa
    12301 aagaaagaaa ttgtcaggaa cttgtacaag attttctttt ctatctattt atactcaatg
    12361 aaaatcaaag agcaaactag gaaactagcc gcaggctgta cttgagtacg gcaaggcgac
    12421 gttgacgcga tttgaatttg attttcgaag agtattattc gtaaaaaatc tcaaaaagcc
    12481 tacctttcgg tagacttagt ttgtttctat tctaatcggc actcttccaa aattttgctc
    12541 tgctatactt ggcttcccta gttggtaaat ctggtctgct ttttgggtca tagcatccca
    12601 aggttctttg ccaattcggc tgactagatt gacctgtcct ttcagagact tgagatgttg
    12661 cctgactttt tcagtaaatc caaggacatg aatggcttct ggcaagacat tttctctagc
    12721 ctgtaccaaa atataggtca ataggcgtct gacacgcgcc ttggtgtaac gtttggtagt
    12781 cactaactcg accaattctt ccacagactg ggctgtttta atagcttctt taatgcgcac
    12841 agccatttct tgattgacct gatagatggt ggttaggtcg ggatttgaca agatttgata
    12901 gcggagcaag ggaaaatagt cttcccagat caccttactg gcctgctcaa atagggcaac
    12961 agaaggcata aaacgttcta agaaatcttg gtccttctga tgttgacgga gggctgtcgc
    13021 cgaggcaaag tccacatctt tattcacaga atggtaacct gccccctgac gctgaatcgg
    13081 atgcagcttg atatttcgtc ctgcaaccgc cttggcatag gccagagcaa gaacatgatt
    13141 gggggtatta cctgaaaaat caagaccagc aaattccttc cacatagctt gggttttctg
    13201 gggataggaa agggaatcag gcagattttt cacaaatttc tccatctcag cacctttctc
    13261 tgtgtataag tcagcgattt tctggtaatc cagaacttcc tctgtcccaa aaactagact
    13321 atcaatgccc aagcgatcca agatatccac agctccttga ccaaagaaat cagctgcctg
    13381 aacactgact aaaaagggca attccactac caagtctgcg ccattttcca gcgccatctg
    13441 ggcccttgtc cacttatcca caatagcagg ctctccacgc tgcatgaaat tccctgacat
    13501 ggcaacaatt ttcagtccct ctgcctgatc cagcaggtat ttatggccat tatgaaaagg
    13561 attgaactcc gcgataatac ctgttatggt catgctaatc tcctacttct gcgccacaaa
    13621 aaaccaacgg gtgctagttt ctgttggctc cttatcctca aagtctgcat agagtttgaa
    13681 ggacttaaat ccagcctgtt ccagcaagat atcataggtc aagatctcat aggtcctctc
    13741 ctcatgtact tcatcgtggc gactaaagga accgtcagcc tccttgataa agaaagtcag
    13801 ctcatgcacg atggagtgag gaacttcgtc ctcatatgta tcccaaagca tggcaaaatc
    13861 ttccgcattt tcatggtagg aatagccagg gaatacttca tctgtctggt aggtcgagtg
    13921 cacgtcaaag atgaaaactc cctcttcatt cagcgcattg tacacgtcct taaagacgtc
    13981 ccctacttcc acctcatcct gcatatagca gatagagtcc gaataacacg tgacaaaatc
    14041 gtatttccct gccttggaca aattcagcat attaccttct ataaaagcaa tcttttgctt
    14101 ggctgaagtt gctctcttct ccgcaatctt caacatatcc gcactcaagt caagtccagt
    14161 cacatcaaaa ccagcctgag aaaagcgcac tgattgaatt cctgtcccac aagccaattc
    14221 caagagtttc tttctctcct tggtcttagg caaatgacgc agagaaaaat tcgtccattt
    14281 gtcgtataaa ctatcgtcca tgaccgcatc atagaccgcc gcaaaggttt cataagtcgc
    14341 cataaaacat gataccaagt ccgcccgtaa ttcgatagaa aattaggagc cttacgatgg
    14401 agccgagctc caaggaaggc tatctatttt ccgagaatta caggacgggt tcaaatcctt
    14461 tctaagatac aaagagcctt aaaagcaaag agtacatagg aaactttctt ccctatcgtc
    14521 agatacaaag aaagttactt tttatctcag cttttagctc gtgttcagtt tcgaaatgaa
    14581 acaaacacga ggccctttac ctttctatca atttttctaa aataagcaaa gaaacccagt
    14641 tgactacaac tgagttcatc ttctaagcaa gagcttctga aatatctact gaattcgcct
    14701 catgccatag cttctctaga ttataatggg cacgcatttc ttctgaaaag atatgcacga
    14761 caacagcacc aaggtccagt aagacccagc ctccagctgc atcgccttcg acatggctac
    14821 ctttaaagcc tgcttgagct actttttcac ggatattagc tgcgatagcg tccaactgac
    14881 ggctattcat tgagctagtg atgacaaagt agtccgtcac actagtcaaa tcttgtacat
    14941 caagtgcgag gatatcctcc gcacgtttct catcagccgc tttcacgact agttctagta
    15001 attctttttc gttcatttaa tcctcttttt agaaaatatg tttgtaatct agaacatctg
    15061 caaagcgctc ctcccaaatg tcctcataaa attcattaat cgtttctgct ggaatgtcat
    15121 ccccatcaaa ggttgtgaca gcaccttctg gaacaataag ctgatagcca tattcaaagg
    15181 caaccttgac agaggtatcc acacaatatt ctgtctgcat accacataaa actaattttt
    15241 caatcccctg cttatccaag tattctttta agccagtttc tttgaaaata ctgttatact
    15301 tcttctgaaa gaccttttca gcaggttttc gatttaaaag cgcagataac tgccaatctt
    15361 ctgatgtttg agcttcagag ttctcaatat gttgaacata gataatttca atattcttgc
    15421 ttctagcctg gttttgtaaa taagaaattt ttcccaatag actttttgtc tggaacccag
    15481 tttctactaa aatattctga acatcaatga ttataaaagc cgtcttcatt tagttctctt
    15541 tcaaatagtg cacaaaggcg ttataggttt caagggtttg gggatagatg gggaatccct
    15601 gatgagctag atactccacg gtacgagctg tttcgtaggc caccgcctta ttgagcgata
    15661 gatttgcaat ctcacgcgcc acatctactc caggaaaggc tcgattgtgc tcgatatagt
    15721 ctgcgacgta gatgacctta tctaggtctg tcatttgacc agcaccgact gtatggactt
    15781 caatggctcg caggatttct gaatcatgca aatccaaatc ttcctgaatt ttgtaaattc
    15841 cgaccatacc atgccagaca ttattgcccc agtttttgag gtcagggtct agctggtaac
    15901 ggtcaatcaa ggctagaaat tcctgatttg acaacttttt agcatagtca tgaagaagac
    15961 ctgctagact tgctttctca acatctacac caaagcgctg agctaattcc atagcggcac
    16021 gctccacacc tagacaatgg gttagacgtt tctcaggtag aaactctgcc attttttcca
    16081 acaaggcctc acgggagcag ttgatatagt cttgatagga catcagtaga gcccctcctt
    16141 ctcgatgtag tctagcactg gctgaggtag gagaaagttg ggtttccgac cttgggcaag
    16201 gaagtcccgc accatgctgg acgagatatc catgagcggt acgtccaccc agataactgg
    16261 ataggaagtc cctaccttgt agcgtggacg ctgaaccccc acaaactgaa ccatgtcaac
    16321 cagttcatca attcggtacc acttaggcag atagtcaacc atgtcggcac cgatgataaa
    16381 gtaataatcc gtatctggat tcttctctgt caaaatcttc atggtgtcgt aggtgtagga
    16441 aatacccttg cgctccaact caatggtttc aatgactagg ccgtcaattc cctcaattgc
    16501 caactcaagc atcttgagac gatggtgttc agggatggtt tcctttttat caacgtgagg
    16561 aggttggtat tcaggcatga gaagaacttg atccagtccc aactgttgcc gtacttgatc
    16621 cgcaacaatg agatgggcat tgtgaacagg gttaaaattc ccccctaaaa tcccaacttg
    16681 tttgcgtttt ttctccttga tttctggctc caactctacc ttggtaaagg gagtcaatag
    16741 ttcgattgtc ataggctagt cccctttatt tctctataaa aacagttatt tggagtttcg
    16801 gtcttagatt tctttgactt tcttagaaat cttgcgattt tctttcttgc tagattgctt
    16861 aaacaaaatc aagatgcgtc ctattttttg gactgtatcc acaccgattt cttcttccaa
    16921 aatttcagct acttcgtgga tgttttcatc tgtgttttgt aagagagtaa ccttgattaa
    16981 ttcacgcgca tcaagagctt gacggacgct ggttttgatt tggtcgttga gtccattttt
    17041 cccgatttgg atgataggtt tgagggtgtg tgcctggctg ttgaggaagg cacgttgttt
    17101 tgatgctaat gacataattt tcttccttgt tttttgatag ttattttagg tggtgaggga
    17161 cggtcggaac taattttcca aagatttcat ctttgaaaaa tggatttcct gacctcacaa
    17221 ttgagccttg gtctcaattg tgccccttgc caatctacag attggcaatc acaccacggc
    17281 aataactatc gctatatgag ctcactttgt tcgctcagcg attttgtttt aaataattgc
    17341 ttttcgtgtg gcgacggcga cgccttctgg tgcccagacg gcgacttttg ctgtgcctgt
    17401 tacacgaatc cagcctaggc ctgaaatgac taggtctgtc ttatctttaa tggtaaagac
    17461 atgctggact agccttggga aatcttcttt ttccttgcta tttggtggtg tcagaagagt
    17521 tcccaggtgc ttatcgtaga aagcactagc tccttcaagc ttgctacgat ggagtttgag
    17581 ttcattatca aagaaagcag taaatccttg cttttctcct gctataaagt caaagcgtcc
    17641 caaaccacct aaaaataggg tttgctcagg attaagctga taggtcttag gcttgatttc
    17701 ctttttagga ctgacatact tgaggttttt ggccgtcaag tagtgagcca tctggtggcg
    17761 gtggataatt cccggcgtat cgtaaatata agatccgtcg tcaagcggaa tctctatttt
    17821 gtccaaggtt gtccctggga agcgtgaagt agtgatgaca ttctgatcac ccgtgatttc
    17881 ttggataata gcattgatta gagttgattt tccaacgttg gtcacaccga ccacatagac
    17941 atcgcggccc ttacggtagt gttcaatctt atcaatgact tccttaatgg catgtttatt
    18001 ttgtgctgaa gttaggacca catcgactgg acgaagacct tcttcatggg cacgtttcat
    18061 gagccactgg ctaatcttac cagacttaac tgacttagga aggatatctt ttttatttcc
    18121 taccaagagg acatcattgc ccgagacgaa acgtggtaaa cctgggatga cagatccatt
    18181 aaaatcaaag atatcaatga cattgaccac taaggcatca ctgtctccca cctcgtgcaa
    18241 gagcttgagg aaatcatcgt tcgtcaactg gacatctgtg atttcattgt agtggcggag
    18301 acggaaacag cgttggcaat agacttcgcc agtctccaaa cctttttcaa gtgccgactg
    18361 gggggtaaaa ccaagaccag ctttatctgt cgtctgaatg gttgctccac aaccaataca
    18421 gagaatttct tccatagtta aattcctttt ttatatgtaa tcggtccgta cttttcagtg
    18481 atttttctca taacacgacg ctcacgagtt cggttaatct gcgttttgat tgagtcatgt
    18541 tggaccaagg gtttgactaa aattgaccga atccctgcac ggtgggctgc tcgtatatct
    18601 gtcatgagct ggtcgccaac catgaccact tcctttttgt catagtggaa ttccttcatg
    18661 gcacggtcaa taccaaatgt gaagggcttc aaggcccagt aaacgtaatc aatcccaaat
    18721 ttctcaactg ctcgttgaac gcgttttttg gtgttatttg acactacgat aatgccaata
    18781 cccgcgtccc gaaggtcatg tagccattgc ttcatctctg gcgttccatc agggttgttc
    18841 caagcaatga gggtattatc caaatcgacc aaaacagcct tgattccctg cgcctgcagg
    18901 cttgggactg tcagatcata gactgcttcc acagcaaaat ctggtatata attttcaatc
    18961 gccattctgg ctccttttct taactttttt tcgcaatttt ccttagtaac aaggataaaa
    19021 caatccacaa acctgcacta gccaaactga tatagagcca agcatggggc tcatctgtta
    19081 aaggtaaggg aacattcatt ccgaaaaagc ctgtcacgac tgccaaaaca gctagcaaga
    19141 ctgaaatgat agtcaaggtt gtcaaattgt cattcagatt attgtttaga atattgttgt
    19201 aagaggctga aagctgctgt aaaatctgag agattaggtc tgtcatggat accagctgat
    19261 gagcctcaat catggcatca tcaaactgtt ctctctcaat ctcatcaaaa ctacgataca
    19321 aggcatgacc ttgaatatgc tctaacaaaa tccgattttg tttggcagct gccgtcagat
    19381 aaaccatacc agtctccaaa tcagacaggg caaagaggtt tttcttggta gttcgctggc
    19441 gcaagaggtc attgacctca tccctactct tgtccatctg ctcaatgaca ggatagtagg
    19501 cattgctgat gatttccaga ctggcaaaga gaaacttata aatcgaaagc gtgtcatggc
    19561 tctccagata acgagtcatc tgttcaatga cataggcgtt cttggtatta ctaatggtaa
    19621 tcaggcgacg atgctcgacg ataaaggtca tgggaaaagt ctcatagtag gccttgtcct
    19681 tttttacgtc taggacatta tagataaagg taaccgtccc actttcacgg tggtagtcca
    19741 tgtgggcgcg ctcgtttcta tccagtgcgt attcaatggt ttccttgtcc aaaccgtaaa
    19801 tttcagaaag gtcttctagt ttattcaact ttcctaggtc taggtctatc caggtacaac
    19861 catggcccaa ctgcttttct aaaaccatct tttctcctta ttaaactccc tctattgtac
    19921 cacaaattac ttaaaataac aggtctagtc tgtgcgtgag aaattgctaa caactgtgat
    19981 atttcggtta gggacaaagt gaagggcttg ctcctcgcta cgaagctgtg tcgtacgaat
    20041 tcccacactg acaaccttac ccgatacagt aatcggtcca tttgtcagaa cgacctcatc
    20101 tcccacatcc agttgacgtt caaagaggat gaaaaagcca ttgatgacat cagacagaaa
    20161 gccttgggct cccataccaa tcgctacccc agcaatacca gctccagcca gcaaactaga
    20221 aactggcaaa cctaaaatcg acaaaatgca gtagagtaaa aagaaatata gcgtataatt
    20281 aaacacattt tctagtaaac gtgagatggt tttttggcgt ccgacatcat gacgagacat
    20341 ttttagagaa ggtttgacaa ttctctgcac catggtatga agcatttttt tagcaatata
    20401 aaatacaatc aaaagcagta aaagagaaat gaccttggtt agaatattct cgataatggt
    20461 tgtcacatct agcttttcaa tataagcctg aataaatttt tgcatataaa actcctttat
    20521 ttcattatac catattttca tcagtctcat tctccataaa tattcaaaaa atagacagaa
    20581 aattcttgat tttgtctatc atttatacta taatcataat cactacacaa aaaggagatt
    20641 attatgaaaa gaatcattca tgcttggaat aaggcaagcc ttgtcaagcg tatcttgatt
    20701 ggtatgcttt gtggggaatc ctaggactga cccttcctaa tctctcaggg attgggctac
    20761 tcggggattt attcgttgga ggtctaaaag ctgttgctcc tatcctagtc tttgctctcg
    20821 ttgccaatgc cctttcccaa catcaaaagg gacaagatag caatatgaaa actgttgttt
    20881 tcttgtatat cctatgatag ggactttcgc cgctgctctt gtagctgtac tagcaagttt
    20941 catcgtccct attgaaatta ccctaaatag tgccaatact gaaattgcac caccagatgg
    21001 gattgggcag gtcctcagca acctcttgct caaactggtt gacagcccag tcaacgccct
    21061 gcttactgct aactatattg gaatcttatc ttgggcagtc atttttggaa tcgctatgag
    21121 agaagccagt aaaaatagta aagaattgct aaaaactatc gctgacgtga cttctaaaat
    21181 tgtcgaatgg atcatcaatc tgactccatt tggaatcctt ggtcttgttt ttaaaaccat
    21241 ttctgacaag ggagtcggaa ggcttgccaa ctacggtatt ttattggttc tattagtaac
    21301 gactatgctt tttgttgccc ctgtggtcaa ccctttgatt gccttcttct ttatgagacg
    21361 caatccttac cctctagttt ggaactgcct ccgtgtcagc ggtgtgacag cctttttcac
    21421 tcgtagttct gcggctaaca ttcctgtcaa tatgaaactc tgccatgacc ttggactcaa
    21481 cccagatacc tattctgttt ctatcccact cggttctact atcaatatgg ctggagtagc
    21541 gattaccatt aaccttttga cccttgttac agttaacact cttggaattc ctgttgactt
    21601 tgccacagcc tttgtcctca gtgtggtagc agctatctca gcctgtggtg cttcaggtat
    21661 tgccggaggt tccctccttc ttatcccagt tgcttgtagc cttttcggta tttctaacga
    21721 tattgccata caaattgttg gggttggttt tgtgattggt gtcatccaag actcatgtga
    21781 aacagccctt aactcttcta cagatgtcct ctttaccgcc gttgccgaat acgcagcaac
    21841 ccgtaaaaaa taactcatca aggcaagcct gctcatgtct tgtcttttac gcttttattc
    21901 taacttatta ggaaattctt atgtctatta gccaacgtac gaccaagctc atcttagcta
    21961 cctgtcttgc ctgcctgttt gcttattttc tcaatctttc gtcagcagtt tcggctggaa
    22021 ttatcgctct cttgagccta tctgatacgc gtagaagtac tttaaaactg gctcgcaatc
    22081 gtcttttttc tatgcttcta gctctggcta tcggtgttct agcttttcac ttgagcggat
    22141 ttcatatctg gagtctcggc ctctatctgg ccttctacgt tcctttagcc tacaagatgg
    22201 gctgggaaat tggcatcaca ccaagcacgg ttttggttag ccatctcttg gttcaagagt
    22261 caacctctcc agacctccta gtcaacgaat tccttctctt tgctatcggt acaggatttg
    22321 ccttgcttgt taatctctat atgccttcac gagaagagga aatccagcac taccacacgc
    22381 tggtggaaga aaagttaaaa gatatcctcc agcgcttcaa atactattta tccagaggag
    22441 acggacgcaa ccgagcacag ctggtagcag aattagacac gcttttgaaa gaagccctca
    22501 gactggtcta tttggatcac tctgaccacc tctttcacca gaccgactac catatccact
    22561 actttgagat gagacaacga caaagtcgta tcctgagaaa catggcccaa cagattaaca
    22621 cttgtcacct tgccgccagt gaaagcctga tcttagcgca actcttttca aaaattgcag
    22681 gtcaactgag ccagaccaat cctgcttctg atttgctaga tgaaattgaa cgttatctgg
    22741 aagtcttccg gaaccgcagt ctgcccaaga caagagaaga atttgaaacc cgcgccaccc
    22801 ttcttcaact cctacgtgaa gccaaaacct tcatccaagt aaaagttgat ttttaccaaa
    22861 aatatagaca gtaatactct tcaaaccacg tcagcgtcgc cttgctgtat atatgttact
    22921 gacttcgtca gttctatcca caacctcaaa acgatgtttt gagctgactt cgtcagttct
    22981 atccacaacc tcaaaacggt gttttgagct gacttcgtca gttctatcca caacctcaaa
    23041 acggtgtttt gagctgactt cgtcagttct atctacaact tcaaaacggt gttttgagct
    23101 gacttcgtca gttccatcca caaccttaaa acagtgtttt gagcagcctg cggctagctt
    23161 cctagtttgc tctttgattt tcattgagta taaaaaagaa aacttcagac caatccacaa
    23221 aaggtgaata tctgaagttt tcttttattt ataagtaatc accataaagg ttaatagtag
    23281 aacaaataaa gccaaactca ccaataaagt gagcaccttg accaaggtgt tgctctgcca
    23341 gtagcttggt ttaccaatat tactagtggc atccatggaa aatagttgat tttgacgggg
    23401 ttgtatagtt accaatacaa tcaaagcaag agataggata atagctaaaa caattagcat
    23461 ttcaatcatt gatctacctc aaaattccca acaaagtaaa gagcaaacca atcagaatca
    23521 ttaagcccag taaaagtgaa aaagttttac taatcttctc ccctcgggtt tctttttcct
    23581 tcttgatggg tttttgtttc aactcttcca tgcgaccttc aacgtctttg taaaataaat
    23641 cttcctctga catgttagcc tcctaaaaca gtttgcatta tttcttgata tcgcaccaag
    23701 tcaacataat ttgcatgttg gccttgtttg ccaagtgcct gacgcatttg atcaacatct
    23761 gaaagggcca atttaatggt ctcaaccaag gcagcaactt cactactttc aaatagatgt
    23821 tctggagcga tataaagtct attgtgaacc gtttggttaa atccaagaat caagagattg
    23881 tgctcaaagg cctgacgcac tgcctgcaac aactcattgc tgtgattgat atccaagtaa
    23941 atatccgaca gttgatacag ctcctgaatc ttctgtggac tagcattctg gtaaaggacc
    24001 acattaggat agcgaagcat gtctaagagc ttagaagaca tctctgtcac tgctgcgata
    24061 cggaaggtaa catcaggcaa ggcttctacg attgcttcta cttgctcaat ctgatctgaa
    24121 ttggtcaaga ttaaggcatc tcgtcttagg aaattatcac gtttgaactg gtaatgataa
    24181 cccaagtgca caaactgatc atggtatttc tcgtcagtta actctaaagc gcgctcataa
    24241 gtcgccttat ttggaatgat gatcttctta gtacgcacat tatcactttc caaaatcaac
    24301 tgcatattac ctggaatggc atcatagaga ggttcctgcc ataccaagac atccgagcca
    24361 gatttatctg gatgatggaa ggaaaccaag aaaggagtcg ctagagtatt aaagataagc
    24421 tgactggtat ctatttccaa atcttgcaaa aagaaggtga taaattcaac tttatttgca
    24481 aagtaacgca tggactgacc aggcaaagtc aataagatat cacccgtcac atggttttcc
    24541 agtaaaactt gttggccatt gacatcttgg taaactgtca taatcggctc gctatctgcg
    24601 ctataagtgg ttttagcaaa gcaagcacca aagcgattgt agtggtcaac ctgacgtact
    24661 cgaccttcta ggtctttcca gtctacctgt ttaaccaagc gagcctgcat tccatcaaca
    24721 taatgaatga cagccctctc ctgcgtcaca tcttcaatac aagcagactg attatctcct
    24781 aaaatttccc aaaaatctga aacgggaact tggttaaaat agagaggttt tccatcctcg
    24841 taacctagat aataggtaaa aggagaaagc agaccatcag gcagaaaacc atctgcatcg
    24901 atgaccactc caagttgaga aagaccagta gcgactagac tttcatgtaa atctctactt
    24961 tcttgactgt aactatcata aagttcaatc atggagcacc tcctctactg ttttcttcca
    25021 cttttctaaa atttctttgg tcaagaagcc ttctgcaatt tgataagaat aggcacgcat
    25081 agcttccaaa cgattttctt gatacaattg acaaatctta gcggcataag cttgcttgat
    25141 ttggtcttct acatggtcag atgaacttgg aatcaaataa ccattttgcc catcctctat
    25201 aaaggtctga ttaccataag gcacatcaaa accaattaga ggtagacctg aaccaatagc
    25261 ttccatcaag gtcagaccaa atccttcgct ggtagaagcc gttaagtaga cctcatactg
    25321 gctataaatc tgcgaaagtt ccgcatgccc cttgagttgg atatagtcct ctgcctgatg
    25381 atttgcaata atttctctaa gcagagaatc ttctccacca ctaccataga tatcaaaggt
    25441 tagttccggt aactccttat gagcttcaat cacagctttc acaagccaat caatgtgctt
    25501 ttctttggca agacgtgaag ccgtaatcaa tgaaaatggt ttgcgccctt gacttgaatc
    25561 tgtcaaggaa tcaatactgc ctacaggaat ggtaacaatc tttggctgat gctgagtata
    25621 tttggcaaat tgctcttgta gaacttcatt ttgtctatca gtagacacga taaagaagtc
    25681 aaccttatct gcattggtaa actgatagtc ataatagtta ttccaaagga tatagtcctc
    25741 atttgtagca ttttcactat aatgctccgc atgaacaact accgctagat gtgctgtctg
    25801 tgcttcctca aacacaacct gtccaatacc tgtctcccta tccagaataa ccaaatcaga
    25861 cttgttcaaa ttcaaagatt tcataaaggc acgcacaaaa gcttgctttc catagaaaat
    25921 cttatccttg aaatgataaa cttcttcctt cccttgattc atcaaaatat catagactgg
    25981 agtcccgtct tcattgtaaa aagttcgttg gtataagact gcaacattgt ccttgggagc
    26041 aaaatactcg ctacaataac gcgtataaga aaagtaatcc ttccgaatca ggtttccctt
    26101 aaaaacatac tcggcatgtt gaaccaagtc cttgttctca tcaaccaaat aacaggttac
    26161 aaacttatct tggtcaaaaa agaatacacg taaaaccttg ccattttttt ctctgtgact
    26221 ttcttcacca ccaaagtaag ccaagacatc atccactgtc acgctagtag gtgcaatttt
    26281 gatatctgtg aaatgattat aaagccagat aacctgatta tcatcaaaac caatattggc
    26341 tgttaagtgc tgaatattat cggctaaaat catatctgta aagataaact tagaggacag
    26401 atttaatttc cgaaaaacac cagcacgata ggcttgagcg tattcaacac cgctactagc
    26461 ccaaccaatt cctaaattta tattgtaaat tgtcatattt tctcctctta tggaaaatga
    26521 gtcacgattt caccttttgg agtgacctca accagcccca tcaggagatt acgcaccata
    26581 tccgcacgga tttgttcttt catagcttca aagcccgcgt aggcttcttg atagtactct
    26641 acgattggat ttttctgact agcagattga ccaccgatag ccatggatag ctgttgtaga
    26701 tagtctacct gctctaccca gttgtcatca atggctttaa gcagtgaaag tcgtaaaaac
    26761 tgttcatata agtcatgttg attaagtaat tctttctttt cagaaagttc tttatcaatc
    26821 acctgcttca taaagctacg aactgcagtt ttgtcagtta catctatata atctggaacc
    26881 tctttaacat gaaaactaat attggtcaca ataaagtgaa acaataattc acgactagca
    26941 tagtgatcag ccgctacctc ttctgtatat ctctcaatga tatccacaac aacatcctct
    27001 aagtcacgag aaccatctat tagacgattt ctctctttat agactatatc ccgttgtata
    27061 ttcatacttt cagcatactc cagagtctga cgacgtgctg aacgtccagc actatcactg
    27121 gcatgctgag ccttttcgac tagtttccgg tatttacgac ctttcaatac ttccggttga
    27181 gtcatatctt gaacctgata gtctttgtac tttttatgca cccaagatgg accaaatttc
    27241 ttgataacat catcctctaa ggatacaaaa aatttactca taccaggatc tccctgacga
    27301 ccagaacggc cacgaatttg taggtcgatc cgctgacttt ccatccgctc agtcccaata
    27361 acaatcaagc ccccaagctc tgcgactcct ttaccaagct tgatatccgt accacgtcct
    27421 gccatagagg tagccactgt cacagccccc atctgacctg actcggagat aatctgagcc
    27481 tcacgcgccg cattattagc atttaggaca ttatgggcaa tcccttcacg aaacaagaga
    27541 gacgaataga gttgagacat ttcaactgag cctacaaaaa cgagtaaagg atttccctta
    27601 gcatggtatt gcttgatgta ctccaaggat gcatacactt tttcaggtaa agtgatatat
    27661 agattatctg gatagtcaat ccgttgtctc ggacgattgg ttggaatgcg tactacagac
    27721 atattgtaag tttcaataaa ctctttttcc gcgaccttac ctgtccctgt cataccagat
    27781 atcttattaa acatcttaaa aagactctga taggtgatcg aggccatagc ccgcgtctca
    27841 ggagataatt tgacatgttc cttggcttca atagcctgat ggagacctcc ttgaagttta
    27901 gtcatttcca ttagacgccc tgttccctta tcaaccagta ccatctcatt tccacgaatg
    27961 atatagtcct tatctttagt aaagagctta tgagctcgaa tcgcataaac caaatgacga
    28021 gcaaaagacg catgctcttc cttgtataaa ttatcaatcc ctaggaaatt ctcagcagac
    28081 ttggccccct tagtagtgag ccaaacctcc tctttctcct ctttaaagat ataatcctct
    28141 ccttcgacca aggttgttac aagtgtatca atgatcgcat agtaattaga ctgaacacga
    28201 ggagaacccg caataatcag aggagtttgt gcactatcaa gcaagatatc atcaatttca
    28261 tcaataataa catagttaaa cggtcgtaaa aacttacctt cttcattcga ggctaggtta
    28321 tcatttagat aatcaaaacc taaactacta ttggttgtgt agatgatatc cgaagcatag
    28381 ataagcttct tttcttcagc tttcatttcc ttctttggat cttccgtaaa tggtacacca
    28441 atggtcaatc ctagaaaacg ataaacttgg cccatttcct cggcatcacg ctttgataaa
    28501 tactcattag gagtcacaac catcactcct tctcctgaaa aagcgttcaa atagacaggc
    28561 atggtagctg tcaaggtctt accttccccc gtattcatct cagcaacatt tccatagtgc
    28621 atgacaatag ctcccatgac ttgaacatca taggggaaca tccctaaaat ccgcttatct
    28681 gcttcacgca ccacagcaaa agcttcaacc aaaatatcgt ctagactttc tccctcggaa
    28741 agacgctgac gaaactctac tgttttagct actaattctt gatccgaaag agaggacatc
    28801 ttgcctttca gggcattaat ctgctttaaa atctttttca ctttcctaag ctggaaatct
    28861 tgacctaaac gtctaaacac ttctgatctc cttgatagaa aaatgataaa aagttaagga
    28921 ctcaaatcca gcacttaaaa gagaaacttg ataagtataa ctatcttctg gataagtaaa
    28981 tatgaaatct ttcgcttttt ctaccttttt ttctaaaata tcaccatagc ggtcaaaaaa
    29041 agttatcttg gtataaactc cattttcagg ctgacaatca aaaaccagtg atagttgata
    29101 ctcctgcttt ctttttaata agggaagctg aggttgggtt cgtgcagcct ggtaattgac
    29161 actagaaaac caagtcttta gaatctcacc agatggcaat agaggattat acagacgaac
    29221 acttttgtca aggtaataag atacagttgt tccatacata taagtcccac caacttctcc
    29281 ccaataaata gactgtctct gtgtaataat catcttttct cccttccaaa accagtctct
    29341 aagaccattc tataaaagtg caaaaaccaa gaaatattgg tatctgtatc atcattgtgt
    29401 cgtccagcag ttcctttaga taaaatcttc gcacctgtat tacacagata agacactaat
    29461 tgttcatagg cttgtggatc catttcttca tctttcatat aggataaacc aaaggttgtc
    29521 tttgaaaaat ttgcttgttt aaaaattttc caaaaacgct gatccaactc cgtcatatct
    29581 tgagaacttg tcccacctgt atgatgaatt aaacaatcaa aagctaagtt agagacaccc
    29641 ggtgcgtcca aacgtccacg actagctata gttcctaaat tagccaaggg cttaccaact
    29701 atgatggcat ggggttcaaa agaagcacca taatagagag ccggaaacgt tcccatagaa
    29761 agacctgata aaatcaaatc cttatgatca aagcctaaat aatcaagata gtgagtgatc
    29821 gtatccttta cttttccctc tagctcatcc gttccgagat aaaaagctcc cccctctaaa
    29881 cgtggatcag aaaataaaat gaagggacat ccaagagttt tcatcataaa gtaaccctcg
    29941 aatccttctg caggtcgata acctgcaaaa tagacagtca aaggcggttt aaaatcacct
    30001 ggatggaaga aatagtttat ttcatcacgc ttggaatcat gtaggatatt tccaccaagt
    30061 acaaacttac caaattgttt tcgactccat cgttggtgaa gattacctag tttgatagtt
    30121 ccacgacctc ttgcttcaac agatatcgag agaaagtagt ctccgtctcg actatccata
    30181 aaaagtgctt gctccaagtc tttttcagat actattactt cttcaaagaa atcatcaaca
    30241 gacccactcc acattttacg gattactaaa cgaaagtcac aattgccttc cttctcatat
    30301 tcaagccaca actcaatagg gagtgttttt tgcaccataa tattataagc ccaataggca
    30361 agctgggcaa aatcttgccc aaactcacct tccaaactga cataatccaa tccttgataa
    30421 gaaatagctc ctgtaaaatt tggattgact tgtatcgtcg gaggaaagag tttatcacca
    30481 tacccaccgc caaaaagaga tttacttaaa tcattgatta gttgttgggg acttaaaaaa
    30541 tcaattgctt gtgcacatcg tttttttaga aaatccacaa cctctctatc atttatttgt
    30601 agattctggt tatagaacaa agtataaggt tcaaaatatt ttacatagga aaataagtct
    30661 ctggaatact gaccatcttc tattaaaact gcatgaaaac ggttaatatc ctctttttcg
    30721 attatttcac gaagggtttt tgatgagttt gggtaaaaat aataccaact catattttca
    30781 ggtatgtcat agtgattttc ccaattggca agtcctatct gtaaaatatt aagttccttt
    30841 gacattttta acctcctcta tccatttctc taattttcca agaaattgtt gtcctgtgtg
    30901 ctccttaatc ttatcaattg aatatatcaa ggactcattc cactccttca acctatctgt
    30961 gtaataatgt gcagccttag aaaattctgt cacatctgtc agcaaataac cattttttaa
    31021 atgttccaca tagacggttt caactaggtt gatttgagga attcctgcac taatcccagc
    31081 aatctgtgtg tagagatgag gatgtctatt taaatccaca atcaaacgga caaattctag
    31141 tgtttttatc aaatctaact catcattcaa gttaacaaac tgtaagcgta agtcctggtg
    31201 ttgattttct tccaggggat tttctgcatc accataatca atcgcctttc ccagattttc
    31261 ggattgaatg ttttcttgga tgaaagactc aacaatccct tcaacctcat tcatttgctc
    31321 ctgactagca gcaaaggctc caaaaatcac ctcagtatcc ttattttcag cgacaaagga
    31381 caagacttga agcagagctt gattatcaat cccctgctca aaatccagtt gaaaataaat
    31441 gatggattct ttcttagttt ggcttcgtcc caatcgtaat cgggtgtcaa aagatgataa
    31501 atgataacat tgatgcattc gttcaggata caactcctgc aacaatcgta aactatcctc
    31561 tctatccacc aaaatcaaat ccgatttttc aaaagttaca tctaaatccc taaaggtatc
    31621 ttgaggatta cgtccaatga acagactcaa cagtttagga ttagtactag gtaaacgatc
    31681 tagtaccaac tggtcatgat gagaatgaga aggaagcata aaaatatcct gatccttcac
    31741 atacgtttgc agataattct caaaaaattc tgccaccaga tctcccatat tttgataggt
    31801 aagcatttta aagcgataac ccaaaattgg attgacttct acctgtcctc cttcttttaa
    31861 acgctctcta aattgccaaa ctcccttagg attcaaatat tcttgatagg ctgcttgccc
    31921 atcttcaaaa aaaataatac tcgatataaa ccctcgatca tccatgatat ggtgcatatg
    31981 tatttgacca tttggttcaa aatactgtat atcactgatg aagccttcaa ctccatgttc
    32041 aaccttagca aatttcttcc catttttttg aacgataatc gtaaagggac tataaataaa
    32101 ttcacagtca tcatcccact caatatctct aatgcttaaa acttgggtat ggagattatg
    32161 aaaatcttgc ataacatcaa aaacggaata agtatccgtt tctaacacac catgtctata
    32221 taaaaaataa cgtagatgag gctggtaagc taatactaat agacgagaat ctatatcttg
    32281 ctcttgaaag agccggatct ggtgaaaggt atcatcaaac tctagacgaa aatgagaaaa
    32341 ataccatgga gtgatatctg catgccatgt tctttctgac ccataccaag ctggaataaa
    32401 ataatacata aaagtctcct aaatcaaagg tttataggtc tcatttagcc ttaaagcctt
    32461 gacctcgtct ctaatcgtaa aaatcatgcc tccaaacatc ataaacaaac ctggaatcat
    32521 tgccaatcgt aataactttt catcaaacaa aacaaaaagc atgggaccac ctgccataac
    32581 cacattaaag agaccaccta tgactgagaa acgaaggacc aatcgattaa taaatcgact
    32641 agtatccgca cctggataaa taccataaat gtattctcca gattttttca tacggtctgc
    32701 aatctcttct ccattcatcg taacaaaagc aaaaatgata ctaaataaaa ataagaccga
    32761 aatataaaca tagacccaca aaggctttcc aatcataaat tcctttgata aagccgctaa
    32821 ccctgaatga ttagggaaaa taaatcccaa caagatgaac aaataagctg gtacactaag
    32881 aaaactcatc acatacatat aaggcatccc acctgcagga ttcaacatga tttcgagata
    32941 aaaatagcgt ttaaatcgat tgtgtaagcc aattttatta acaggaacca aatagcgagc
    33001 tcgatacata agggcaagta aataagaaaa gacaagggtt aataaagcaa gtaacacaat
    33061 aatccctgtt ggaatgtgta ctgtctgaaa tgtttccaaa acatcctgag gaatatttaa
    33121 aaccatactg gataggagaa tgactataga acctccaatc cccatactcg catttaaatc
    33181 tgacaaccaa acaagaaaaa atgttcctgc tatcagcaat attgtattca ttagaacaac
    33241 caatattgca gaataggagg attgtactgg cagtctcaag ctaactgcca atgattgaat
    33301 gacagctatc atcaaggtca ggtacatttt acggcgatct tgtatttcta tagacgtaga
    33361 tgttaaaccc aaccgtttag aaaaagaaaa catctgccat aaaatcatag ctgacatcca
    33421 aggggacaat ccaacagaaa aaattgacaa acttcttaga tttccacctg ttagggcggc
    33481 tgagaaggct agataggctg ttgaacctcc taaaaaatct ttagtattta ggtcaacaaa
    33541 agggagagta atacgacttc ccaatacata gataaacagt agaaataagg taaatagtcc
    33601 tttttttact gctattgacg aataaatctt agtcataaaa cactcccctc accttcattg
    33661 tttgtgatat ggtaaataac aattctttta tttcaacttt taccatttct ttgttctgca
    33721 tcaaaaaaca actttaatct gaaacaaata tctttatatt cctcagtatc tgttaattga
    33781 tggtattcta aagtttgctt ataatctttt aaaagattga caaatcgaat acgcagaacg
    33841 gagacatcca aaccagccaa gacacaatcc gatattttct tagaaaacac ttcaacaata
    33901 tctcttgccc aggaaagatc aaattccgtt ctcatgatgc ttcctgaacg tcttctatac
    33961 agatagagat tatcgtttat aaagacgatt ttagaggcta aaagataaaa gcgatgcata
    34021 gttgcttcat catcaaagcg gcgaccattt gaaaagtgaa cttcattgaa taattctttt
    34081 ttaatcaact taaatgtcgg caatataaag gccgagctat tgaatttcca atctcctgct
    34141 tgacggttca taatttcttg tatagctaat tcttcgacgc aaaaatcttg ccctgttaca
    34201 taaaaataaa aattcccgtc actttcgtca taattatagt aattccctat aacaacatct
    34261 gcatcctttt cttggacttg ctgataaaga gtttcaatca tattttcttt tacgatatca
    34321 tctgaatcaa caatcgtgat gtactctccc ttagctacac taatccccat atttttagct
    34381 tgtgctgccc ccccttattt tcttgatgca atactcgcac acgattatct tgcatcataa
    34441 aagcatcaca aatttcccca gaattatccg tagaaccgtc atcaactaaa ataatttcta
    34501 tattttgata ggtttgcttc agaatatgct cgacacactc cactaaatat tttcccgtat
    34561 tgtatatcgg taccacaaca ctaattaatt cactcattac tctatctcta tattcttcat
    34621 tctatccact aaatcatctg gtctctccac agaaaaaata ccgtcataga gtccatcatc
    34681 catactttta cgtgtgatat caaaagcgaa gattttcttg ccactttctt gtgcccttgt
    34741 aactacatta aaaacctctc cacctgtatt aatatctaaa taaactatag aatcgtccaa
    34801 tagccaatca attctactat gtaatacatt ttgatatact gttacattcg tatactgtga
    34861 tagagaggtc agctcctcag aacaatcaca cggtgcagcc aaatgaaaat gccaatcagg
    34921 taaccgttgt actaaatact ctaaatgttt tatatctgca ctccatgtca caagcatcac
    34981 ttgtttttta gactgattgc ttctttcaaa ataatttaaa tcggaacgtt gaaaagcaat
    35041 ctctgaccaa tccaaatctc tataaaccca ccataattca cgtagtctag atatactata
    35101 tgtattccaa ggtttatcat gactagcata gtgaacaatt gtaggtggat tgtcatctag
    35161 acgttcacat tcttgagcaa ggtgataaat atcaacaccc accatataat tatatgtttt
    35221 atctaaggct agccaattat cctcaaaata aatatttaaa atactctgat ccccaagatg
    35281 aacaacttga ttttgctcgg ccgctaattc caataaacta ttgacaatag agtgttcttt
    35341 ccattttgca acatccatta gtaacatacc agcattaaat ccagattttc gtccttcata
    35401 ggcatagaca tcatcaacag caccaattga atatcctttg agatctatct caaacaaagt
    35461 agctagttcc ccagttacaa tgatatcgga atccagatac aataccctat cagattcaac
    35521 cacttctgtc gcaaaaaatc taaagtaaga agcataattt atatgaggtc ctgttttata
    35581 gctttcaaaa agttctttat caatgtgtac actgcgaatt gtagaattca caacttccat
    35641 ttttctcccc aataatttaa accattcagg agctatatca ctgttgagaa tataaaaatc
    35701 aacatctcta ttgtgataca atacagactt tatagtcgtc tctaaaggaa taagataggc
    35761 attatctgcc gctaatacta ttgattttct catactgttc tctacctcat gtaagatctg
    35821 attgctgcaa ccattttttt cggaatggag cttggataga taccttcata tgaaatctca
    35881 gaaagatttt cagagcaagt attgtcgaaa gctatcattg gttttttgta cttaagcaca
    35941 aactcatatg catcttctaa ttttcgatca tgattcaaat ccaaatacaa atctgaagct
    36001 attactattt gttccaataa cattggtaag gaatttggat atacggtaac attaggaaaa
    36061 cgcgttaaag ctagtagttc atttgccata tttgtataag caacaatatg aaaatgaact
    36121 tctggcaatt gctccgccaa ataatcgatt ttctcgacac accaagaatt cgttaaatta
    36181 acacattgta acttaaatat ttgtgattta ctaggatact tcacactctt tgaaaaccat
    36241 tcatttaaaa taacagacca atccatcaaa gagtattccc accaaacttc tcttagacgt
    36301 ccaacagaaa attgattcca aggcttatcc tgagaaatat agtgtaaaat tagtggcagt
    36361 ggttcgagcg gaatatcaaa aatgaattga tgtttaaagg ctgccgcccc ataatcatat
    36421 cctatttgaa aattatattg atcttcaagg gaactatatt gatctttaaa caacatattc
    36481 aaaattgact ggtctccttc ttccacattc tcatgttctt tttctgttaa gtcaatcaat
    36541 ttttgtcgaa tagtttcaga tccccatttt ttgttgttaa tcaagagaac accagcattg
    36601 aagccgactc ctgctccaaa gcaagaacga gctgctgcca aataattttc acctaagtct
    36661 aattcaaaca aatcggtcaa atcaccagtc acaatcaaat cactatctag atagagaact
    36721 ttatcttctg ttacaaaatc tggaataaaa tagcgtgcaa atgtcatatg attgatatga
    36781 ggtaatttat tagaccaatt catttgaaac tgtgagccaa ttaacttgca gtcaatcaag
    36841 tcgcccccca tctcttgtaa atatattctt atttgactaa accattcctg aggaatgtcc
    36901 tgatttagca gataaatttt caaatgacta ttgtgtctac agagtgactt catcgccgtt
    36961 tcgatttgtc gaatataagc gtaatcacct gcaaatgcta cagcgcgttt ggtatttctc
    37021 attttaaatc cttatcattt attttaaata gtctagttta atattcgtgc aacaatctga
    37081 ctgttatagg tttcatcatc aataaattca atatcttgat caaaattata ctctgcagta
    37141 tgtttatgag aaaatttaac cttagtttta gctcccattt tcatccattc atactctgag
    37201 tcaatatggc ctacatccaa aacttgatag cccatctggc atagattata actcagaact
    37261 tttgctgtag gacccagcat acataaaatc aagcgaccac cagcatactt ttcaatttct
    37321 tgttcaattt catgaactct agaaaaggca ctatgagaag gacagataat tcgcttaata
    37381 gaatttgcct catcgaataa atcatttccg acacctgaac gagaagtcgc accttcgact
    37441 atcagtaagt cacggttttc ccaaatgctt ttcaattttt caaattgagc tttagcttga
    37501 ctcttgtctt caaaatcgat ataagggcga gatacaaagg ttgagccata ccatgaatca
    37561 ctgcataact ctctgtaaaa atccatataa tgatccatgt gatctttcca aaatggaatc
    37621 gcccacgatg taaacctaaa acgatctgta aaagcatcag gaaggcacac tactaaatct
    37681 tctcgacttt cttggccgat aatgtccctc atgattgaaa ccaactcttc atcataatcc
    37741 tggtagggaa ttgaatgccc tgcaagcata ttgatttccc catctccaaa acgaactaaa
    37801 gaagagttgt gttcaataat ataatccaag gtttcatcta tccctttcac ctggattata
    37861 tcaagcggtt tttctagcat atccttagtt atcaattttt gcaaatcagc caacattcgt
    37921 tcaggatgtt cacttgaata caacctttgg ccattattcc cattttgaga tttataaaag
    37981 gctagtatag gtttagatag atgagccatt tctgcaacga tatcggatgt tgaagtcagt
    38041 aggttgatat ctaaataagc atccatcttc tccttcaact cgtctagctt atctctagaa
    38101 attgccggat agagctgaat attaggataa cgattagaca aatcatataa ttttggcccc
    38161 atagctgtcc aagcagcaac ttgaaaaaca acatttggca actgtccagc caagtaatcc
    38221 aattgttcga tatcctgaga agccgtaaac gttaaacaag ttaaacttgg aagttgtcga
    38281 aaacaggtaa gatgtccgtt tttcttaaaa tctttaatcg tatcaatcat aaaggaaact
    38341 ttcccatttt cgaaaacgat ttgtcctaac tctccgtgct gagtcgattg aaaagctagt
    38401 agcgtttttt cttgttcttg caaagatttt aaaaagttct catctgcact accatgattg
    38461 atatccaaat atagatttgt tgactttttc agcttatcta atacaggagg aacaatttgt
    38521 ggatgcaatc tcacattatc atacacagct aattttatca gcttatcacc catatccgtc
    38581 caagcagcaa tatgaaatac cacatctggt aaagctgtga ctaactcttc tattccttct
    38641 aaatcttggg aatttgtcaa agttaaacaa gaaaattcct tatctaaaga cgatgtcagt
    38701 tcaaactccc ctatatgatg ttgtaaaatt ttagtccatt ccaaatcatg aaattcccac
    38761 cataaatcac gataacgatt ggctatcaag gtggtccagg gttttctgta agacacaaaa
    38821 tgaataatac taggaggtac taactcatta aaataatttt cccatttatt ataaaaagca
    38881 gttacatcat gacccacttg aaggttaaat tctttatcca gctctaacca atcatcttgt
    38941 aagacctgat taaaaatcgt ttgatcacca ttaaaatgct cgaaacggcc ttcttcaact
    39001 tccttcattg taacaatgct ctgttcaatt agtcgttctt ttaatttctc ttgacgccat
    39061 tttttattgt tgattaataa aacacctgca ttaaatgtaa ttccatctgt atctttaact
    39121 gctgcgagtg atttttcttc taaacaaata ctaaataatt tctctaaaga agtatttaca
    39181 atcaaatcac tgtctaaata taaaacctta tcttcttgga tataatctgc aataaaatat
    39241 ctagtatagg taatgctact aatgtgatct tgcttttccc aatcttgaaa cacagtttgt
    39301 tcaggtagct tcacatcgat aatctcacta cctaacatgc gagctatttt tcgtggtttg
    39361 cgaaaccaat ctggcataat atcttgattt aaaatataaa tcttaacatc tcgattgtgg
    39421 tataaaatag attttatggt tgtttctaac tgcctgatgt aattacgatc accagctaat
    39481 acaattgttt taatcatctt gttctcttat tcaactccca aatggtaaat tgcatctacc
    39541 aataattttt tagtaaaata accttctttt agcaaataac taaaggtttt tatccgattc
    39601 gtcatttctt gatattcttg tagattcatt ttatcaacta tcgcatgaac ttcttctaga
    39661 ctattcgcca caaagcctaa gccttgatct actataaatt tagcagttga caagctactt
    39721 ggtacaatga ctgggatgcc cgctgtcaga taggtactca ccttatgaga tatatttaag
    39781 gtataatatt ggttgctttc tccatcgttt tgataagttc cccagacaag gccaaatcca
    39841 ccctttgata attctagcaa caattcctca tcttttttcc atccttcgat gctgagattt
    39901 ctagcactag aactagcttc ccctttattt gaaaatactc tcaaaggcgt atcttgagac
    39961 caattttgta agtctggaaa acgctctaaa cttccagcaa aaaaaagttc ttttttaaaa
    40021 gcaggggtgt ataaggataa atcatgagga tgatcccaca tcccttgaac aagaatcttc
    40081 ttagtcgtca atccttcttc catcaggcgt gttttcattc tctctgacgg cactatcaaa
    40141 acatctgata gattatacat gtacatatag tctttcatga gataatagtt actatcaaac
    40201 atgaggggaa caacatcatg gataaagcaa ataattttca cctgcatatc cttcaactta
    40261 tcaaacaaga gacgatcaaa ttcaaagcca ttccaggttg gagactgaaa gactaaaata
    40321 tccccaatag aaatactggc cataatacca tccagacgct tattcatttc agaaggacta
    40381 tctgaagcaa tgttgtaaaa ataaatacca acctctctaa atcccagttg actagctatc
    40441 ttttgaacag cattttgagc taagataacc gtactatcac cagccatgcc gtataaattt
    40501 gttaaatgta gtttcatatt acgctatctc attttttatt actatctacg gaagtaaaga
    40561 caatttttcc atcattcttc tataaatctc tgtgaactgc atattttgtt cttctaattt
    40621 tgctatcact ctttgtaatc tatttttata aatcaaaata tgattggtca agtcatagtc
    40681 tgaacttgct aaaatagcta tcttttcttc cctagccccg atttcatcat acatacgctt
    40741 ttcactccat gtattcgata aagtatcaga taaaccaaca cggtaccagt aaacacaacg
    40801 attcaaatag actatcctcg aagctcttag aagtaccttc catgtccagt aagtatcctc
    40861 tgcaattttt cctataggaa atggtaaatc ttgtagtaac tctctcttga ataacttcaa
    40921 gacagctaca gtccagttcc catttctgac ttcttcgaca ccttccctat ccatgattgc
    40981 tttaccttct atcacttcca gagaatcatc tggatccgta acataagtca tatacacata
    41041 gcgtgtttca tcataagaat tataacgccc gatactaata tctgcgtttt cctttttcaa
    41101 agcaccatat aatcggtcta aagcatcatg ttccaaccaa tcatcagagt ctacaaaagt
    41161 aatgtacgcc cccccccgaa cattcaatac ctaggttacg agctgatgaa agaccgccgt
    41221 ttgctttctc aaaatatttg aaacgagaat ctttctctac aaattcttca catattttgg
    41281 atgaatgatc tggagagcca tcattaacca tgatacattc aaaattcgga taagtttgtt
    41341 tttgaatact atccaaacac tgtcgcaaat aattctcaac gttgtagatt gggactacaa
    41401 tactaattag ttcattttcc atatactaac tttcctagac ttgatttatt ctatttccct
    41461 cttaatctct tctctttaca acaagatttc cattattata aactacactg tttgatggaa
    41521 tatcattgcg tatgagacaa cctgctccaa taatggtatt atccccaatc gtcactcctt
    41581 ttaaaatcgt gacattactt cctatccagc agtctctgcc aactctaatc ggcgctgtag
    41641 tccactgcca cttttcaatc ttttcagcag tataaatatg gtcgtgatca taaaaacgaa
    41701 caccctctcc catcatggta ccagctccga tttcaattct ttccatacag ttaaaactac
    41761 aagagttgtt gataaaaaca ccatcatgca gtattaactt accagaagag acatgaaaat
    41821 tttcaaaatt tctgcagatg acatttgctc ctacaatgaa gtgagcctta tcatcgattt
    41881 taaagacatt ccaagtccca tcttggatga ttttttcttg atgattggtg cctactctga
    41941 cttcattcag atagtctctc actctagaaa ccaatttgtc tggtgtactt gatagaaaaa
    42001 cttcctgccc ctgatttcca tggactgtat tgtcaaaagc aaagacagat tttccagcct
    42061 gtacaaactt ggagacaata gcatctactt cttgaaagtg attgatatct agtaaaaagt
    42121 cacaggtatc gactagaaag tttagctgtt cttcatttgt aatcgttcgg agacggacat
    42181 tatagttcgt ttctaagcta cggattttct cggaaaattg aacaggtgct gcaatataaa
    42241 aacagatatt tggcaaggat tcaaggagat aatccaactc ttcaatctga tcactaacag
    42301 tcaaaatcag agcatctcct actaaatgat ttttcttcat ttgcttattt ctctcaattt
    42361 ctcaatcatt gcttgaactt ggtcaacagc atatacctcc tgacctgctt cataggtttt
    42421 ggtattttca aaggacaaaa tagtttttcc aagtcttgca aattgattga taatttcctc
    42481 tgttttttca ccatgattaa tatctaaaag tacttgactg gtttctacca attcattatc
    42541 gacatctacc aaatagtgaa ttccgttaaa tatagtcacg tttggataaa ttgtcatctg
    42601 agccaatcga tcactaacta ttactctagc tgctatctta aactgaatat caggcaagga
    42661 ttgaaccaat gtctcaattt gttcaatatg gtctgaggca gtatagatta gacaagtgaa
    42721 aggttccttt attggataga tgtgagatct ttgtaatgga tgtaaatgat ggttttgtcc
    42781 caattctgtc cattcaagcc catgatagta ccaccaaact tcacgatagg tttgggccgc
    42841 caaatctttc cacggtttcc gatgagaaag atagtgaata atagcaggat aatcctgacc
    42901 ctcaggcaat tgataatcag caaactgttt atgaatgaca atatgattat aatcaaagtc
    42961 caattccaac catttatgtt caaaaagcat attcaagatg ctctgatctg cctgatccac
    43021 cttatcatgc cattcattgg ttacatcaat taatttttgg gtcatattct cttttttcca
    43081 aaaagcattg tttaccaaga gaacaccggc attaaagatt tcttgaccaa aataagctct
    43141 gcccccaaaa tctctaacag cagccaaagg ataatcttgt aagtctgtag caaacaaatc
    43201 atcaagattt ttcgttacaa gtaaatcaca gtccaagtag agggccttat cttcttgcac
    43261 gaaatcagct atgaaatagc gtaaaaaaac tgtgtaacta atatccgttt tataacatga
    43321 aatttgctca gaagttaccc gacaattaat aatttctgag tcaaacttct ctaagcgctt
    43381 atttaattgc ttaatccatt catttggaaa atcgctatga atcagataaa aacgaatcga
    43441 acgattatga taacaaatag acttgattgt cgttaaaact tggtctacat agccatagtt
    43501 tgctgctagg acaatggctt tcttttcact tgattcttgt accgttaact gatttaaaag
    43561 tctttttttc atttcaaact ctttatatat tgctgtgtca gataaaccac tagcttgacc
    43621 gttggcgaga ctgacttcca acatctgacg ataaactgcc aagtgtttct ctagaggata
    43681 tcccatatta gctagtagcg taatacgttc agacatagca tcaactaagg cgtgcatcca
    43741 cttttctgtc caaactcttg atagactacc ttttctaatc cgataagcat aaagaccttt
    43801 atttaaataa attacctttt ccgataataa atataccttt tgattgaggt aaccatcttc
    43861 tcctaattta cccatgtcaa agcgcaactg ctcaaacaat cttgccttat agagtttacc
    43921 acaagcagat atcaaagcaa aactcttcat ttcttgagtt tcataacaag ttctcaaaga
    43981 tggaaacatt gtcatatacc ttatcataat aggagtctcc tgatatatga aagtagaaca
    44041 ttccttcact ttcgttgaaa gaatagtaat tcccaactgc aatatcagct tgatattctg
    44101 ttattttttt atatagggtt tctacataat ctaactcaat ccaatcatct gaatccacaa
    44161 aggtcacata atttccggac atattattca gaccggtgtt tcgtgcggca gaaataccag
    44221 cattttcttg ttctatatag agaattcggt gatccatttc tgaaaattct ttacaaattt
    44281 cacctgaagc atccgtagaa ccatcattaa cgacaacaat ctcaatattt ttatatgttt
    44341 gagaaataat actatctaga catttcctca gatagttttc tacattgtat actggtacaa
    44401 tgaccgtaat tttatcatcc actagcaatt ttctccttga tttatgtgcg cgagttagtt
    44461 tagcctaact ttacaggttg tcttactctt cttcatcacg tttacggcgt ttcgcaacca
    44521 atccaatacc tgtaacagct gctagaactc caagtaacac agatccaatt gacgactcag
    44581 tacctgtatt aggcaattct ttttgggatt tacctgtcga tccagaagca tttccaactt
    44641 gtgagttcga atggtttgct gaatttgaaa ctgatgtact tgctgaggct gacgcactcg
    44701 ttgatgctga ctcagacgca ctggttgatg ccgattcaga cgctgaggtg cttgctgatg
    44761 ctgaggcact ggttgacgct gaagctgagg cgctagtact tgctgaagcc gaggcactgg
    44821 ttgatgctga ggctgaggcg ctagtacttg ctgaagctga ggcacttgtc gatgctgagg
    44881 ccgaagcact ggtacttgct gaagccgaag cactcgttga tgctgaggct gaagcactgg
    44941 ttgacgctga agctgaggca ctcgttgagg ctgaagccga agcactcgtt gatgcggaag
    45001 ctgacgcact ggtacttgct gaagctgagg cgcttgtgct tgctgaggcc gaggcacttg
    45061 ttgaggccga ttcagaagct gaggtactcg ttgatgcgga ttcagatgct gaggtgcttg
    45121 ctgaggccga ggcgctggtt gatgcagaag ctgaggcact ggttgacgct gaagctgagg
    45181 cactggttga ggccgattca gaagctgagg tactggtgct tgctgaagct gaggcactcg
    45241 ttgatgccga ttcagacgct gaggtgcttg ctgaagctga tgcacttgtt gatgctgagg
    45301 ctgaggcgct ggttgatgcc gattcagacg ctgagatact tgctgaggct gaggcgcttg
    45361 tacttgctga agtcgacgca ctggttgagg ccgattcaga tgctgacgta cttgcacttg
    45421 ttgacgccga tgtgcttgca cttgtcgatg ctgacgtgct tgccgatgta cttgtgcttg
    45481 ttgatgctga cgtgcttgct gaagccgaag cactggtgct tgctgaagcc gatgcacttg
    45541 tacttgctga ggttgatgcc gattcagacg cactcgttga tgcggaagct gacgcactgg
    45601 ttga
//
LOCUS       DAVBBS010000009        43671 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVBBS010000009 DAVBBS010000000
VERSION     DAVBBS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43671
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.009"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            1..225
                     /locus_tag="VQU32_000494"
                     /pseudo
     CDS             1..225
                     /locus_tag="VQU32_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            222..440
                     /locus_tag="VQU32_000495"
     CDS             222..440
                     /locus_tag="VQU32_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472006.1"
                     /translation="MSISPRFEALEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     TAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(497..781)
                     /locus_tag="VQU32_000496"
     CDS             complement(497..781)
                     /locus_tag="VQU32_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HET2472007.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(835..1770)
                     /locus_tag="VQU32_000497"
     CDS             complement(835..1770)
                     /locus_tag="VQU32_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET2472008.1"
                     /translation="MEICQQILEKIKEYNTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNVTDAETAAIVGAPGRIDSVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            1874..2317
                     /locus_tag="VQU32_000498"
     CDS             1874..2317
                     /locus_tag="VQU32_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HET2472009.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            2433..2699
                     /locus_tag="VQU32_000499"
     CDS             2433..2699
                     /locus_tag="VQU32_000499"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET2472010.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSYILKRSRDYQDQNIK"
     gene            2696..3070
                     /gene="crcB"
                     /locus_tag="VQU32_000500"
     CDS             2696..3070
                     /gene="crcB"
                     /locus_tag="VQU32_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET2472011.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYFSLV
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            3064..3393
                     /gene="crcB"
                     /locus_tag="VQU32_000501"
     CDS             3064..3393
                     /gene="crcB"
                     /locus_tag="VQU32_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET2472012.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            3512..3859
                     /gene="rplS"
                     /locus_tag="VQU32_000502"
     CDS             3512..3859
                     /gene="rplS"
                     /locus_tag="VQU32_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET2472013.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            3902..3973
                     /locus_tag="VQU32_000503"
     tRNA            3902..3973
                     /locus_tag="VQU32_000503"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3935..3937,aa:Arg,seq:cct)
     gene            complement(4140..5138)
                     /locus_tag="VQU32_000504"
     CDS             complement(4140..5138)
                     /locus_tag="VQU32_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HET2472014.1"
                     /translation="MNFFSKLFNLKQNNHNRDTNSDCNNFYLNELECGLTPGQLILID
                     WTQKTGRNYNFPRYFKYSLQIDPESTHNQLYKLGYFTKNKTLSYLTVVELKTILSKHN
                     LATSGKKAELITRIINNVNIDNLDIPFEFKLTKEAQNLIIEHSDYIKAYYDKDISMED
                     YCKEKNNISFKATFGDIKWSLLNKQAHRNTVSGDFGCLSNTRKAQGRHLEQEGNIKHA
                     LIYYIESLIITISGLENNFSATDYPVYYPDSIPDYSLKHIQTLMESLSDDDYDFAFDE
                     ALFRFSILNANHFLSKEDIDYLRVNLPRSTAEEINNYLKKYECYSPLNNLELDDFE"
     gene            complement(5181..5987)
                     /gene="yidA"
                     /locus_tag="VQU32_000505"
     CDS             complement(5181..5987)
                     /gene="yidA"
                     /locus_tag="VQU32_000505"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HET2472015.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRPMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(6000..7304)
                     /locus_tag="VQU32_000506"
     CDS             complement(6000..7304)
                     /locus_tag="VQU32_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET2472016.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTVTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPVENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKNFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESQNPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            7376..7753
                     /locus_tag="VQU32_000507"
     CDS             7376..7753
                     /locus_tag="VQU32_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HET2472017.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELMMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            7844..8176
                     /locus_tag="VQU32_000508"
     CDS             7844..8176
                     /locus_tag="VQU32_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HET2472018.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            8188..9759
                     /gene="ffh"
                     /locus_tag="VQU32_000509"
     CDS             8188..9759
                     /gene="ffh"
                     /locus_tag="VQU32_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET2472019.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(9954..11237)
                     /locus_tag="VQU32_000510"
     CDS             complement(9954..11237)
                     /locus_tag="VQU32_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HET2472020.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(11540..12253)
                     /gene="rsmG"
                     /locus_tag="VQU32_000511"
     CDS             complement(11540..12253)
                     /gene="rsmG"
                     /locus_tag="VQU32_000511"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET2472021.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            12347..12907
                     /locus_tag="VQU32_000512"
     CDS             12347..12907
                     /locus_tag="VQU32_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET2472022.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTSEEEKSVPKVDFSGLGD"
     gene            12909..13808
                     /gene="htpX"
                     /locus_tag="VQU32_000513"
     CDS             12909..13808
                     /gene="htpX"
                     /locus_tag="VQU32_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HET2472023.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(13852..15411)
                     /locus_tag="VQU32_000514"
     CDS             complement(13852..15411)
                     /locus_tag="VQU32_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2472024.1"
                     /translation="MHYRHSRKGNSMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKVPEDLKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKTAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLAQELQLTVRGQEKIGIIGPNGVGKSTLLAKLQQLLNDKREISLGFMPQDYHK
                     KLQLDLSSIAYISKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(15650..15967)
                     /locus_tag="VQU32_000515"
     CDS             complement(15650..15967)
                     /locus_tag="VQU32_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HET2472025.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSPLIGLGSLKLNDQARTAWRNYDKIHYEHVKHVLSLYGPV
                     FGF"
     gene            16146..16688
                     /locus_tag="VQU32_000516"
     CDS             16146..16688
                     /locus_tag="VQU32_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET2472026.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            16688..17317
                     /gene="nth"
                     /locus_tag="VQU32_000517"
     CDS             16688..17317
                     /gene="nth"
                     /locus_tag="VQU32_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET2472027.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            17528..18049
                     /gene="pyrR"
                     /locus_tag="VQU32_000518"
     CDS             17528..18049
                     /gene="pyrR"
                     /locus_tag="VQU32_000518"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET2472028.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            18068..18991
                     /locus_tag="VQU32_000519"
     CDS             18068..18991
                     /locus_tag="VQU32_000519"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET2472029.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            19041..20120
                     /gene="carA"
                     /locus_tag="VQU32_000520"
     CDS             19041..20120
                     /gene="carA"
                     /locus_tag="VQU32_000520"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET2472030.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            20432..23608
                     /gene="carB"
                     /locus_tag="VQU32_000521"
     CDS             20432..23608
                     /gene="carB"
                     /locus_tag="VQU32_000521"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET2472031.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(23883..24692)
                     /locus_tag="VQU32_000522"
     CDS             complement(23883..24692)
                     /locus_tag="VQU32_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2472032.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDQFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNIPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(24694..25497)
                     /locus_tag="VQU32_000523"
     CDS             complement(24694..25497)
                     /locus_tag="VQU32_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2472033.1"
                     /translation="MKQLTVEDAKQIELEILNYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPMDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRLVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSATFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            complement(25507..26994)
                     /gene="tacF"
                     /locus_tag="VQU32_000524"
     CDS             complement(25507..26994)
                     /gene="tacF"
                     /locus_tag="VQU32_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HET2472034.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGDILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            27330..28037
                     /locus_tag="VQU32_000525"
     CDS             27330..28037
                     /locus_tag="VQU32_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET2472035.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDSIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            28042..29064
                     /locus_tag="VQU32_000526"
     CDS             28042..29064
                     /locus_tag="VQU32_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET2472036.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            29081..29950
                     /gene="licA"
                     /locus_tag="VQU32_000527"
     CDS             29081..29950
                     /gene="licA"
                     /locus_tag="VQU32_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HET2472037.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            29940..30818
                     /locus_tag="VQU32_000528"
     CDS             29940..30818
                     /locus_tag="VQU32_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET2472038.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            30830..31519
                     /locus_tag="VQU32_000529"
     CDS             30830..31519
                     /locus_tag="VQU32_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET2472039.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQNIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     HKLEEILKNEN"
     gene            31617..32465
                     /gene="dprA"
                     /locus_tag="VQU32_000530"
     CDS             31617..32465
                     /gene="dprA"
                     /locus_tag="VQU32_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HET2472040.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            33349..34392
                     /locus_tag="VQU32_000531"
     CDS             33349..34392
                     /locus_tag="VQU32_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HET2472041.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGHNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFYTESEEQ"
     gene            34559..36646
                     /gene="topA"
                     /locus_tag="VQU32_000532"
     CDS             34559..36646
                     /gene="topA"
                     /locus_tag="VQU32_000532"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET2472042.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            36867..37226
                     /locus_tag="VQU32_000533"
     CDS             36867..37226
                     /locus_tag="VQU32_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HET2472043.1"
                     /translation="MRIIYLIIGFLSLTLAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQAYVADFRETKSIARERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            37319..37951
                     /locus_tag="VQU32_000534"
     CDS             37319..37951
                     /locus_tag="VQU32_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HET2472044.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            37995..38651
                     /locus_tag="VQU32_000535"
     CDS             37995..38651
                     /locus_tag="VQU32_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET2472045.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVYMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            38670..39764
                     /locus_tag="VQU32_000536"
     CDS             38670..39764
                     /locus_tag="VQU32_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET2472046.1"
                     /translation="MNFQLAKYSLLKRFLENIGFTTLEEYGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKILLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALVDKKQEITDADI
                     RALVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEFNATGQGSVEAIF
                     NAIDKFFNQSVRLVSYTIDAVTDGIDTQDRVLVTVENRDTETIFNAAGLDFDVLKASA
                     IVYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            39776..40813
                     /gene="leuB"
                     /locus_tag="VQU32_000537"
                     /pseudo
     CDS             39776..40813
                     /gene="leuB"
                     /locus_tag="VQU32_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
     gene            40810..41079
                     /locus_tag="VQU32_000538"
     CDS             40810..41079
                     /locus_tag="VQU32_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HET2472047.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKSKARRRVWRIPEKILLIL
                     AFTFGGFGAWLAGIIFHHKTRKWYFKIVWFLGMVTTLVALYFIWR"
     gene            41083..41442
                     /gene="leuD"
                     /locus_tag="VQU32_000539"
     CDS             41083..41442
                     /gene="leuD"
                     /locus_tag="VQU32_000539"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET2472048.1"
                     /translation="MAGSSREYAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            41604..42172
                     /locus_tag="VQU32_000540"
                     /pseudo
     CDS             41604..42172
                     /locus_tag="VQU32_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019771122.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            42266..42331
                     /locus_tag="VQU32_000541"
                     /pseudo
     CDS             42266..42331
                     /locus_tag="VQU32_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            42494..>43671
                     /locus_tag="VQU32_000542"
     CDS             42494..>43671
                     /locus_tag="VQU32_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HET2472049.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELMSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     "
BASE COUNT        13251 a         7809 c         9434 g        13177 t
ORIGIN      
        1 tccgactaat attggctttg catttattcc tccatacaca ccagagatga acccattgaa
       61 caagtgtgga aagagattcg taaacgtgga tttaagaata aagcctttta aactttggaa
      121 gatgtcatga atcaactcca agatgttata caaggactgg agaaggaggt gataaagtcc
      181 atcgttaatc ggagatggac tagaatgctt tttgaaagca gatgagtata agtcctcgtt
      241 ttgaagcctt ggaacaggcc attgcctcca aagatttgga aaaagtccga gaagctttta
      301 agaaaatgaa ttccacttgg accatcaatg aaagtgtggt tcgtgacaat agtacagctc
      361 attatggacg tgttgaaaca gctatttcct tcctgcctag tagcatggaa accgaaccta
      421 cagatgagtc tggaacatga aaacagcagt tgatttcaag aaacaaaaaa gaacagtcga
      481 aactgttctt ttatcattat ttgagaccgt attttttgtt gaaacgatcc acgcgtccat
      541 ctgcttgagt gaacttttga cgtccagtgt agaatgggtg tgagtctgat gaaatttcca
      601 cacggatcaa tgggtaagtt tcgccttcga actcaactgt ttcgttagag cgttttgttg
      661 aaccgctaag gaattggtaa ccagtagttg tgtccatgaa gacaactggg cgatattctg
      721 gatggatatc ttttttcatt ttgcaatatt tcctttctgc catggtctct ttgcgagcca
      781 tagattgtta ccatactagt ctatcagatt ctgaaaagtt tggcaagtat tttatcagtt
      841 tttaagcaag ttttttaact tttggtagat gatttcattt tcttctaggc tataggaatt
      901 agcaccactt gctagagggt ggcctccacc atcatgctcc ttggcgattt cattgatagg
      961 atggatttta ctgcgtaagc gaacgcggta gtggccatca gcctgttcca caaaaattcc
     1021 ccagagacta acactatcaa tacgcccagg tgcacctaca atggccgcag tttcagcatc
     1081 ggttacattg tattgtttca agattttctg actcaggata acgcgagcag caccattttc
     1141 atccacttcc agatggtcgt agatgtagcc ttgcagttta gcaattttgt agctcatagt
     1201 gtccattttg cgagtgagag ccgcaaagtc aaagttatgt tctctcaaat aagcagccag
     1261 gcgaagagtc cgtgcagtgg tagaagggta gaggaagcga cctgtatcac cgacaattcc
     1321 tgcaaagagc aactcagcat cgcgatctgc caaggctagt tgggttgttt gggcaaatag
     1381 ggtaatcatc tcgctagcgc tacttgaact agtatcgacc caagacaggt caccgtatac
     1441 atcatcattt ggatggtggt caatcttaat gagaaaatca ccttgactat agcgcttatc
     1501 atcgatacga gcagtattag ctgtatcaca gacgatgaca agtgcgcctt ggtaggctct
     1561 atcttcaaca agatccatct cagccatcca agtaagagtt ggttcatcaa aaccgacggc
     1621 tttgatggtt ttttctggga aatgatgttc cagcaaggct ttcaatccca cctgacttcc
     1681 caaggcatca gggtctggtt tcatatgacg atgaatgata atggtgttgt attctttaat
     1741 tttttctaaa atttgttggc aaatctccat aaataacctc aattctttct catttcattg
     1801 tagcacaaga atttttattt ttaaaatgaa aaagatgtga tataatggag aaagataaat
     1861 tgaggaggac gatatggcat tagcaaaaat tgtatttgcc agtatgaccg gtaataccga
     1921 agaaattgca gatattgtag cagacaaatt acgtgacttg ggcttggatg ttgatgttga
     1981 tgaatgtaca actgttgacg cttcagactt cttggaagca gacatcgcta tcgttgcgac
     2041 ctatacttat ggtgatggtg aattgccaga tgagatgatg gacttctacg aagacctagc
     2101 agatctcaac ttgaatggca aaatctacgg agtggtcggc tcaggagata ccttctacga
     2161 tgaattctgt aaggctgttg atgactttga ccgtgtcttt gtgtcaacag gagcagaaaa
     2221 aggttcagag tgtgttaaag ttgatctttc tgccgaggaa gaagatattg aacgcttgga
     2281 acaattcgca gaagaattgg ctgctaaagt aggataagca taaaagtgcg ctgatgtaat
     2341 caatcagtgc atttttctta tcgagagttg ggattttact agcctaattg atggcttttt
     2401 gatacaataa ttcaaataaa ggaggagact gtatggattt agatattatt cgtcaagaaa
     2461 tcgatcaaat cgacgaccaa attgtcaaac tgttagaaga acgaatgcat ttagttgagg
     2521 gggtggttgc ttataagaaa gcttcaggta aaccgatttt agatactaag agagaagaag
     2581 ttatttttga aaaggttagg agtcgtgtag aggacaagcg ctatcaggag actattgtcg
     2641 cgactttttc gtacatactc aaacgttcgc gtgattatca ggatcaaaat atcaaatgaa
     2701 aaaagaacaa ttttatccgc taggaatttt tctagctgct atgttgggcg gacttgtccg
     2761 atatctagtt tccacctggt taccagccag tccagacttt ccttggggca ctctctttgt
     2821 caactatctg ggaattttct gcttgatttt tcttgtcaag ggctatctgg tctataaggg
     2881 gactagtaag ggcttgattt tagcactggg gacgggtttt tgcggaggtt taacaacttt
     2941 ttccagccta atgcttgata ctgtgaagct gcttgataca gggcgttatt ttagtttagt
     3001 cctgtatttg cttttgagta tcggtggagg cctgctttta gcttactatt tggggaggaa
     3061 gaaatggtaa tcgtctatct tgcaatcgcc tgtggcttgg gagcactcgt acggtatttc
     3121 ttttctcgct ataatcaagc ttctaaattg ccactcggaa cgctcatagc taatctttta
     3181 ggttgttttt taattggagt attctacaat catgtagagt ctaaggaagt atatgctatt
     3241 ctagcaacag gattttgtgg cggtttaaca actttttcga ccttgaatga cgaacttcaa
     3301 agactgctaa gtgataagaa ggtcttttat tcttacctga ctttgactta cataggtggt
     3361 ttggttgcga tttttttagg aattttgcta taaatttctt tacttttttt tagaaatttg
     3421 gtaaactata tcttgtgtgt aatggacgca caaaaataca gtttatccgc tgaggaagat
     3481 tcctcaagat tgacaaagat aggagaataa aatgaatcca ttaatccaaa gcttgactga
     3541 aggtcaactt cgtacagata tcccatcatt ccgtcctggt gacactgttc gtgtacatgc
     3601 gaaagttgtc gaaggtaacc gtgaacgtat ccagattttt gaaggtgttg ttattgcacg
     3661 taaaggtgct ggcatctctg aaaactacac agttcgtaaa atctctaacg gtgtaggtgt
     3721 tgagcgtatc ttcccaatcc acactccacg tgttgaaaaa atcgaagttg tacgttacgg
     3781 taaagtacgt cgtgcgaaat tgtactactt gcgtgctctt caaggtaagg cagctcgtat
     3841 caaagaaatc cgtcgttaat tcgactaaaa aactctgctt tttcagcaga gtttttatct
     3901 agctccctta gttcaatgga tataacaact ccctcctaag gagtagttgc aggttcgatt
     3961 cctgcagggg gcatgtaatt agatatcaaa gaagtttgtc aaagagtctt tgatattaaa
     4021 agaacgcata atatagggga ttgtctccat aatattatgc gttttattat tgaaaaattt
     4081 gggcaatcat cagataaaat tgagactata tcgagtatat aaatgtttgt atagtcaatt
     4141 tattcaaaat cgtcaagttc taaattattt aaaggactat aacattcata tttctttaag
     4201 taattgttta tttcttcagc agtggaacga ggtaaattaa ctcttaaata gtcaatatct
     4261 tccttagata aaaaatgatt tgcattcaaa attgagaagc gaaataatgc ttcatcaaaa
     4321 gcaaaatcat aatcgtcatc agataatgat tccattaatg tttgaatatg ttttagtgag
     4381 tagtcaggta tcgaatcggg ataatatact ggataatcag tggctgaaaa attgttttct
     4441 aatcctgaaa tagtaattat caaagattct atgtaatata ttaaagcatg tttaatatta
     4501 ccttcttgtt ccaaatgtct tccctgagcc tttcgtgtgt tagataagca tccaaaatct
     4561 cctgatacag tattcctatg agcttgttta tttaagagac tccattttat atcaccaaaa
     4621 gttgctttaa aagagatatt gtttttttct ttacaataat cttccatact tatgtcttta
     4681 tcatagtatg ctttgatata gtcactatgt tcgataataa gattttgtgc ttcttttgtt
     4741 agtttaaatt cgaacggaat atctaaattg tcaatgttaa cattattaat tattcttgta
     4801 attaattctg ctttttttcc agaagtagct aaattatgtt tagataatat agtttttaat
     4861 tctactactg taagatatga taaagtctta tttttagtga agtatcctaa tttgtataat
     4921 tgattgtgtg tagattcagg gtcaatttga agagagtatt taaaatatct tgggaaatta
     4981 taatttctcc ctgttttttg agtccaatct atgagtatga gttgcccagg agtaaggcca
     5041 cattctaatt cgtttaggta aaaattgtta caatcagaat ttgtgtctct attatgatta
     5101 ttttgtttta aattaaatag tttgctaaaa aaattcattg gattttattg ctaatcaatt
     5161 cttattgaaa aatgatactt ttacagtacc catgttcgga tggcatgggc aacgccggat
     5221 tcgtcatttg ttttggtgat gtatttggcg atttttttga tttctggatt tccattttcc
     5281 atgacaacgg ggtttccaac gacttccagc atgggacggt cattttcttc atcaccgatt
     5341 gccatggttt catctttggt caatccgagt ttttcagcca agtgagtaat ggctgaaccc
     5401 ttgtctacat tctttttaag gagttcgagg tagaaaggag cagatttgtt gatggagtag
     5461 cgctcgtaaa attctgctgg aattttttca atcgcagcat cgagaatttc tggttcatcg
     5521 ataaacatac atttaacaat ttctttgcca gccatttctt caggggtacg gtagaagata
     5581 ggcatgctga cgagggttga ttcgtgtaca gtgtattttc cgatattgcg atttgcagta
     5641 tagataccgt ccttggtaat ggcatgcatg tggacaccga gcttgcgact gaggaattcc
     5701 atatccagat aatcctcata ggtcaaggat tcgctgataa tctcatggcc agtagcagtt
     5761 tcttggacaa gggcaccatt gaaggtcacc acatagtcac cttggtctct caactgcaag
     5821 tcgtccagaa gtttggcaac acctgcgatg gggcggccag ttgcaatcac gactttgaca
     5881 ccagcttctt tggcatcttg gatggcagaa aagacttcag gagtgatttc cttttggctg
     5941 ttgactaggg ttccgtcaat atcgacggca attagtttaa tactcataaa attcctcttc
     6001 tagtttttat ttggggtaaa atgatcgttc tcaatcaagt gtaaaaattg ctgggtaatg
     6061 cttgcgaaga tgctgttttg gtctaacatt tctttaggga aatagaaacg attatctccg
     6121 tggcgactgc cagcaaggga ttggacgata ggagacaggc tagagagttc ggccagttct
     6181 ccattttttt gtaaaatctc aatctgtgtc cgtggatttt gagattcggg acgatagata
     6241 tcataaggga ggtcaaagtt cttatgaatg gcagtatagt agtcgggatc aaagccgatg
     6301 tcctcaacca attttctcat gctagtaagt tgatcttggt cctcttgtga aaaggtaatg
     6361 gatttaaaga ccttgcggtt gacaaagcgt tgagataaat ctgcaagaat tttgtcagga
     6421 ctagtcatcc aaagctggaa gtaggtattc atcacgccat catccagagc cagatagtca
     6481 gtcaaggtca catttttttc gaagaaaggc aggagatgtg gggaagttcg tgcaaagaaa
     6541 ttcttgtcct cagggtagag ttccttggcg cgcttgagaa gattctgtag gagaacttcc
     6601 atggcgcgtg ttgctgggtg gaaataaacc tgcatgtaca tctggtagcg actgaggacg
     6661 tagtcttcga tggcgtgcat gccattgcgc tgaaaggcga taccattttc gacaggacga
     6721 atgactcgga ggatgcgagt caggtcaaat tccccatagg atgctcctgt aaaataggag
     6781 tcgcgcaaga gatagtccat gcgatctgcg tcaatctgac tagaaatgag ctgcacgacc
     6841 tgcttattag gataggtatg gtcaatgaca ctggccacct tttctgggaa atcaggtgcc
     6901 acttgtagca ggacttggtg aatctctgtc tcaggatttt gaataatctc ctgagtaatg
     6961 gcttcatggt ctgtatcaaa gagattttca aaagtatggg agtaggcacc atgcccaagg
     7021 tcgtgtagga gagcagcggt cacggtcaag agagactcgg caggattcca ttcctcagga
     7081 tatttttctt cgaaaatctc tgtgatgcgt cgtgcaattt catagactcc tagacagtga
     7141 gagaagcgac tgtgttctcc accgtggaag gtataactgg aagttcccag ttgtttgatc
     7201 cggcgcaaac gctgaaattc ttttgtatta atcaagtcat agatgatttg attattgaca
     7261 tggatgtagt tgtgaactgg gtcacggaat actttttcgt tcatatgacc attatagcaa
     7321 aatcctgctc tttttggtaa aatagtagga caagagacaa gaaagagggc ctgatgtgaa
     7381 gattcggatg cgaaatacaa ttcagtttga tgagcagttg gaagtgattg accagcttta
     7441 tgatgtggaa gtgcatgaaa aaggagatta tagctacctg cttttctata atgaggaaaa
     7501 ggaaaaagta gtgattaaat ttcatggtca agaacttatg atgagccgat tttctaatcc
     7561 caagaccatt atgcgctttc taaaggatag cgatagttta gcctatattc ctacacctat
     7621 gggcatgcag gagtttatca tccaaacgag ccattatcaa gttgataggc aaaagattga
     7681 actagattat caactacaaa atcaagaggg acatcctttt gccagctatc aattggaaat
     7741 tacttggggc taggctcatg tctttttcaa atttagctat ctatcttctt ggatcggctt
     7801 cctagtgtgg tagattaggc taatttttgg tataataaaa gttatggaaa tcgaaaaaac
     7861 caatcgtatg aatgcgctct ttgaatttta tgcggcgctt ttgacagata agcaaatgaa
     7921 ttatatcgag ctctactacg ctgatgatta cagccttgct gaaattgccg aggagtttgg
     7981 tgtcagtcgt caggctgtct atgacaatat caagcgaaca gaaaagattc tggaagatta
     8041 tgagatgaaa ttgcacatgt actcggacta tattgtccgc agtcagattt ttgatcagat
     8101 tttggagcgc tatcccaagg atgactttct gcaggagcag atagaaattt taacaagcat
     8161 tgataataga gaataagagg aagaaaaatg gcatttgaaa gtttaacaga acgtttgcag
     8221 aacgtcttta aaaatctacg taaaaaagga aaaatctctg aatctgatgt ccaagaggca
     8281 accaaagaaa ttcgcttggc cttgctcgag gccgacgttg ccttgcctgt tgtaaaggac
     8341 tttatcaaga aagttcgtga gcgtgcagtc gggcatgagg tcattgatac acttaatcct
     8401 gcgcaacaga ttattaaaat cgttgatgag gaactgacag ccgttttagg ttctgatacg
     8461 gcagaaatta tcaagtcacc taagattcca accatcatca tgatggttgg tttacaaggg
     8521 gctggtaaaa caacctttgc tggtaaattg gccaacaaac tcaagaaaga agaaaatgct
     8581 cgtcctttga tgattgcggc ggatatttat cgtccagctg ccattgacca gcttaagacc
     8641 ttgggacaac agattgatgt gcctgtcttt gcacttggaa cagaagtacc agctgttgag
     8701 attgtacgtc aaggtttgga gcaagcccaa actaatcata acgactatgt cttgattgat
     8761 actgcgggtc gtttgcagat tgatgagctc ctcatgaatg agcttcgtga tgtgaaagca
     8821 ttggctcaac caaatgaaat cttgcttgtc gttgatgcta tgattggtca ggaagcagcc
     8881 aatgttgcgc gtgagtttaa tgctcagttg gaagtgactg gggtcatcct taccaagatt
     8941 gatggcgata ctcgtggtgg tgctgctctg tctgttcgtc acattactgg aaaaccaatc
     9001 aagttcactg gtacaggtga aaagattacg gacattgaaa ccttccaccc agaccgcatg
     9061 tctagccgta tccttggtat gggggatatg ctcactttga ttgagaaagc ttctcaggaa
     9121 tacgatgaac aaaaagccct tgaaatggct gagaagatgc gcgaaaacac ctttgatttt
     9181 aatgatttca tcgatcaatt agatcaggtg caaaatatgg ggccgatgga agacttgctc
     9241 aagatgattc caggtatggc caacaatcca gcccttcaaa acatgaaggt ggatgaacgc
     9301 cagattgctc gtaaacgtgc cattgtgtct tcgatgacac ctgaagagcg tgaaaaccca
     9361 gatttgttaa atccaagccg tcgccgtcgt attgctgctg gttctggaaa tacattcgtc
     9421 gaagtcaata aattcatcaa ggactttaac caggctaaac agctcatgca gggtgttatg
     9481 tctggggata tgaataaaat gatgaagcaa atggggatta atccaaataa ccttcctaaa
     9541 aatatgccaa atatgggagg aatggatatg tctgcccttg aaggaatgat gggacaaggc
     9601 ggtatgcctg acttatcagc tctcggagga gcaggaatgc cagatatgag ccagatgttt
     9661 ggtggcggtt tgaaaggtaa aattggtgaa tttgccatga aacagtccat gaaacgtatg
     9721 gctaacaaaa tgaagaaagc gaagaagaaa cgcaagtaag acaagagatt gatgcaaatc
     9781 tcatttgatg agaaaataaa gtaaaaattt ctatgataaa acgtacagta tcaaggtttt
     9841 agaatatctg atattatacg ttttttgatt ttaataattt tgttaagaca cttttattat
     9901 ggtatacgaa aaatccactc agctgtctga gtggattagg tgtatttaga ctcttagtct
     9961 ttattttcgt atggcaagat caagttcaag atgattcctg tcatggctga aagggcagta
    10021 cctgaaagtg taactggacc aagtttaagg atagctcctc caagtccaag aaccaacata
    10081 gcacttgcga tgatgaggtt tcgcatttga gcgaaatcaa cacgttcttt aatcaagact
    10141 ttcaaaccat tgctggcgat aaccccatag agaaggattg acataccacc aagtacagcg
    10201 tttggaatag ttgaaatcaa ggcagtgaat ttaccaagga agctgagggc aatcgcgatg
    10261 aaggcagcgt tacggataac tgagacagaa gcgatacgag tcataccgat aacccctgta
    10321 ttttctccgt aagttgtatt ggctggtcca ccaaggaagg cagaaacaga agttgcgata
    10381 ccgtcaccaa gaagagtacg gtgaagacct ggttctttta agaattgacg accacagatt
    10441 tgacccaaaa cagtatggtc tccgatatgt tcagaaattg ttacgatagc gattggcaag
    10501 atagcgatgg cttctggacc aaagtaaaga ttgtactctt taaaggcacc acctgtgcta
    10561 aatggcaagt agaaaccagg aatttcgaac cagttggctt taagaactgg tgtaaagtca
    10621 accaagccaa gagttagtgc gaaaaggtaa ccaccgataa tggcaaagag gaatggaatg
    10681 attcgtagga agccttttcc ttttgtattg ataaaggcag caattaggaa agtaacaacg
    10741 gctaccagag catttttcca atttccgtct gctacaagac ctgcattggt aacagctgaa
    10801 cctgcaagtc caagaccgat aacgatgatc ataggaccga taatgattgg tggcaagagt
    10861 ttatcaatcc attttgttcc tacaaatcgg atgctggtag caacaaggac atagaccaaa
    10921 ccagtcaaga taacccctgt ttgggcagca gatacatccc cccccatttc tttcatagcc
    10981 agtgacatag ctgtgataaa ggcaaatgaa gaacctagat aaactggaac tttaaaacca
    11041 gtagcaatca tgtagatgag tgttccaaca cctgaagcaa aaagggcaac agatacaggc
    11101 attcccaaaa tcaatggtac caagatggtc gcaccaaaca tggcgaaaac gtgttggaaa
    11161 ctaaggagaa ttccttttcc agccgaagga cgttggtcaa cgtctagtaa caaatcaaca
    11221 gttgattctt gtttcatata aacctcactt ttgggcatca aaaaagagcc cattatttta
    11281 cagcaatagg ccctcagagt aagacttctt taaaatttag attttaaaga gttttaaata
    11341 tttctgcgtc ttcgtcacct cacgggatga cattaaaaac ctttgcttag gatagtatag
    11401 cagaaaaaaa tgattttgta aatcattttt tcccgagcct agaaataaaa gagcgaggct
    11461 gattttgtaa atcatttttt cccgagccta gaaataaaag agcgaggctg attttgtaaa
    11521 catttgttta ctaaaaaatt taaagtgggc gtttatttgg cataccagcc ttacgcggat
    11581 atttatttgg tgtttctttt ttcttttcta ccactgtgat atagcgcgga tctctattcg
    11641 gtagggcgta gctgagattg tcttcgacct tactaaaaag gagattgagg gcattcttag
    11701 cttctaataa ttcctcaggc gcattgctgg ccttgagtgc caatagtttt ccaccaacct
    11761 taaggtaggg aatggtcaat tcagatagga cctgcatacg ggcaaccgca cgagctgtta
    11821 caaaatcata ttgagcacgg aagttcttgt cttgggcaaa atcttcggca cgtccgtggt
    11881 agaaatgaac tccgttcaaa tccagttctt gagccaagag ttgtaggaag ttgatgcgct
    11941 tattgagtga atcaataatg gtcacatcta actccggata gagaattttc attggtagac
    12001 taggaaatcc tgccccagcc ccgatatcaa gaagtttgat agtttcattg ggaatcaaac
    12061 cttgaagaat gggtgcaatc gaatcgtaaa aatgtttgag ataaacttct tccttgtccg
    12121 taatcgccgt caaattaatc ttctcattcc actcgaccaa gagctcaaaa taacgttcaa
    12181 attgttcttt ttgctggtcc gaaagtggaa gattctgctc ggcaagcaag ttgtaaaatg
    12241 tttttggttt catagttatt tccttcttta gtatcatctt attttaccac aatcatagaa
    12301 aatttgatat actggtacta atctaaaagc agaaaggact tatattatga cttggattat
    12361 tcttggagtt atcgctctta ttgttatttt tgtgattgtt agctataacg gtttggttaa
    12421 aaatcgtatg caaaccaagg aggcttggag tcagattgat gttcagttga aacgtcgaaa
    12481 tgacctcttg ccaaacttga ttgagactgt aaaaggttat gccaaatatg aaggttctac
    12541 ccttgaaaag gtggcagaac tacgtaacca agtggcggca gcgacttcac cagcagaagc
    12601 tatgaaagcc agtgatgccc tcactcgtca ggtttcaggt atttttgcag ttgcagaaag
    12661 ctatccagat ttgaaagcta gtgctaactt tgttaaattg caagaggagt tgacaaacac
    12721 agaaaataaa atttcttact ctcgtcaact ctataacagt gttgtcagca actacaatgt
    12781 aaaattagaa actttcccga gcaatattat cgctggaatg tttggattta aagcggcaga
    12841 tttccttcaa acatctgaag aggaaaagtc ggttcctaaa gttgatttta gcggtttagg
    12901 tgactaagat gttgtttgat caaattgcaa gcaataaacg aaaaacctgg attttgttgc
    12961 tggtattttt cctactctta gctcttgttg gttatgcggt tggttatctc tttataagat
    13021 ctggacttgg tggtttggtt atcgcactga ttatcggctt tatctacgct ttgtctatga
    13081 tttttcaatc gacagagatt gtcatgtcca tgaatggagc gcgtgaggtg gatgagcaaa
    13141 cggcaccaga cctctaccat gtagtggaag atatggctct ggtcgctcag attcctatgc
    13201 cccgtgtttt catcattgat gatccagcct taaatgcctt tgcgacaggt tctaatcctc
    13261 aaaatgcggc tgttgctgcg acttcaggtc tactagctat catgaatcgt gaagaactag
    13321 aagctgttat gggacatgaa gtcagtcata ttcgtaatta tgatatccgt atttcgacta
    13381 ttgcagttgc ccttgctagt gctatcacca tgctttctag tatggcaggt cgtatgatgt
    13441 ggtggggtgg agcaggtcgc agacgaagtg atgatgaccg agatggaaat ggtcttgaaa
    13501 tcattatgct agtggtttcc ctactagcta ttgtactggc acctctcgct gcaaccttgg
    13561 ttcagctcgc tatttctcgt cagagggaat ttctggcaga tgcatctagt gtcgagctga
    13621 ctcgcaatcc ccagggaatg attaatgccc tagataagtt ggacaatagc aaacctatga
    13681 gtcgccacgt cgatgatgct agcagtgccc tttatatcaa tgatcctaag aaaggtgggg
    13741 ggttccaaaa actcttttat acccacccac ctatctcaga acggattgaa cgtttaaaac
    13801 agatgtaaaa gaatccagag gtcgtctctg gatttttgct atgttgcaaa tttataaatc
    13861 ttctaaatta actagcttca aaccgtgttc tgtcatgcga tagatgatcg agcagacttc
    13921 ttttaagaaa cgacggtcat gcgaaacagt gatgagaccg cctggatagg ttgcaaagag
    13981 ttttctgatt tggggttgag aagtgggaga aaagtttcgt gtgggttcat ccagcaggag
    14041 aaagtttggt ttgcgcagaa ctagatccaa aagcaggagt tttccctgtt gtccgccaga
    14101 taaggagcga atttgatgct gcatttctgg ataactgaaa ttgagactgg ctaggtgaga
    14161 ttggattttc tgaagttcct ctttttcccc agttttgctg atataagcta ttgaggataa
    14221 atccaattgc agttttttgt ggtaatcttg tggcataaaa ccaagtgaaa tctctctttt
    14281 atcattcaga agttgctgta atttggctaa cagagttgat ttcccaacac catttgggcc
    14341 gatgataccg attttttctt ggccacggac agttagttgc agttcctgag ccaaaacacg
    14401 gtcgtcaatg gacaaatttt ctttttccag ttggactaag actttagaag ctggtaatgg
    14461 ttggatgtct gaaaagaaaa gttggatttg ttcctcttca agtggctttt gagtcatgga
    14521 ctgagctgtc ttttcgtagc gtttttcttg tgagaggaca gttttcatct ttttagccaa
    14581 taggcgaccg gcagtactat ctttggtagc tcgaagcgca gtttctacat tttgcttaac
    14641 tctccgatgt ttttccatgg ttttatcgta ggctctttgg ttgttagcag cttgctgact
    14701 ttgtttggca aaattagcct ttctctgctc actatagcta tcatagtcta aatgctctac
    14761 tagcgtttcc gcttctttac ggtgtttgac cagtcgcaag tgaacaatag tgtctgccgt
    14821 ttcagaaaga aagtcttcat catgggaaat gaaaataacg gtttgcctgg tcttttgaat
    14881 ctggcctttt agccaatcaa ctgtctcaag gtctaggtca tttgaaggtt catctaaaaa
    14941 tagaatctca aagggtttgg ctaactcatg gataagctga attttcaaag cttcgccccc
    15001 tgatagattg ccaatctctt ggtcacttgc gaaacgattg ctatcaaaat gcaattcctc
    15061 cgccaaacga tagaggatac tgtagtctaa atcaatagaa tctaaaaaga agtagtcgtg
    15121 taaagttttc ttttttaggt cctcagggac tttttgagga atgtaggcca gtgactgata
    15181 atcagactgg atgtctccct tgatagtgaa atcagacaaa tcttccccca ttaaagtttt
    15241 aagtaaggtt gatttaccat ttccttcttc gccaataata gctacctttt ccccgtcttg
    15301 tatggtcatg cttaggtcag agacaaggtc tcgtaaatct ttgttttgtg tgatggtcag
    15361 gtgattgatt tttatcatac tattgccctt tctagaatgt cgatagtgca taacaaaaag
    15421 ctctacttta cctagtagag cccaaagtct atgtcaaaaa aactctatta acatgccaga
    15481 aaaagagcat aaaagagcac acaaaaagag acaaaaacaa gatctactaa ataaaggttc
    15541 tttatttgat agacttagtt gttcatgtct cccttacctc cgagtattag tatctttata
    15601 ctatactttt aaggaaattt tgtcaacaga aaactggaaa ttctatgctc taaaatccaa
    15661 aaacaggtcc atagagactt agtacgtgtt tgacatgctc gtaatggatt ttatcatagt
    15721 ttcgccaagc agtgcgtgct tgatcgttta gttttaggct acccagtcca atcagaggac
    15781 tggtgggttg gtaaaatttc tcaagatcaa ttaagatact gttgttcaac ttttccgttt
    15841 ttttgagctc ttgcatagta gtgtttagca gctcttgtag tcgaatatca tctgccgtac
    15901 cctgtttaca gttttggtag cttttattta acttggatag gagttgaatg agtttcttgt
    15961 cttccatgtc ttccatatga gcatcctcct tgatctgatg tactctttcc ctatagtata
    16021 gcactaaaaa ataaaactgt catagtaaat atgttaaaat tttgaatttt aattacaaag
    16081 tttggtgtgc tgatttgtag ccttttcatg gtataatcaa gagagtaaat ctttatggag
    16141 gaagtatgaa attaaatatt caagaaattc gtaagcagtc tgaaggtttg aactttgaac
    16201 aaacgttaga tttagttgat gacctgcgtg cacgtaatca agaaatttta gatgtaaaag
    16261 atatccttgc agttgggaaa gtacaatatg aagaccgtat gtatttctta gattatcaac
    16321 tatcttatac cattgttctt gcttcgagtc gcagtatgga gccagttgag ttagttgaat
    16381 cttatccagt cacggaagtt ttcatggaag gcgcaactaa ccagctagat caagaagttt
    16441 tagatgatga cttggtcttg cccattgaaa atggggagct tgaccttgct gagagtgtat
    16501 cagacaatat cctgctaaac attcctatca aggtcttgac ggctgaagaa gaagctggtc
    16561 aaggatttat ctcaggaaat gactggcaaa tcatgacaga ggaagaatac caagctcaac
    16621 aagcagtaaa gaaagaagaa aacagtcctt ttgctggctt acaaggacta tttgacggag
    16681 atgaataatg gtcttgtcaa aaaaacgagc acgaaaggtg ctagaagaaa tcattgccct
    16741 cttcccagat gccaagccta gtcttgattt taccaatcat tttgaactcc tggttgcggt
    16801 catgttatca gcccagacga cagatgcagc ggtaaataag gccacaccag gtctctttgt
    16861 tgcctttcca acaccccaag ctatgtctgt agcgacagag agtgagattg cttcacatat
    16921 ttctcgcttg ggattgtatc ggaataaagc taaattcctt aaaaaatgtg cccaacagtt
    16981 actagacgat tttgatggtc aagtccctca gacacgtgaa gaattggaga gtttggcagg
    17041 tgttggtcgc aagacagcca atgttgtcat gagtgtagga tttgggattc cagcctttgc
    17101 agtggatact catgtggagc gtatttgcaa acaccacgat attgtcaaaa aatcagcgac
    17161 gccacttgag gtggaaaagc gggtcatgga tatcttgccg cctgagcagt ggttagctgc
    17221 ccatcaggcc atgatttatt ttggaagagc catttgccat ccaaaaaatc cagagtgtga
    17281 ccagtaccca caattatatg attttagcaa tttgtaagat gctacattga aactttttgc
    17341 ttgattttaa gcatgctttg tgctatagta aatgataact aaatattcct ttaaacctgt
    17401 cccgtgaggc aggcaaggag cgcgataaag tagaagggtg aagtctatac tgtttgcagt
    17461 agggctcgct tggctataaa tttcaagtct ccttgtttaa ggagactttt ctttttggag
    17521 gtttgtcatg aaaacaaaag aagttgtaga cgaattgacc gtcaaacgag cgattacgcg
    17581 tattacttat gagattatcg aacgcaacaa agatttgaat aagatcgtct tggctggtat
    17641 taaaactcgt ggtgtcttta ttgcccaccg aatccaagaa cgtttgaagc agttagaaaa
    17701 tctttcagtt cctgttgtgg aattggatac taaacctttc cgtgatgatg ttaaaagtgg
    17761 agaagatact tctttggttt ctgtcgatgt gacagaccgc gaagttatct tggtggatga
    17821 tgtgctctat acaggtcgta ccatccgtgc tgctattgat aatattgtcg gtcatggtcg
    17881 tcctgcgcgt gtgagtttag cagttctagt cgatcgtgga catagagaat tgccaatccg
    17941 tccagattac gttggaaaaa atatcccaac cagtcgttct gaagaaatca tcgtagagat
    18001 gacagaactt gatgaccaag acagagttct gattactgaa gaagcttaga aagttaaagg
    18061 agtagccatg tcagaaaatc aacaagcatt gaaccatgtg gtgtccatgg aagacctcac
    18121 tgtcgatcaa gtaatgaaat tgatcaagcg aggaattgag tttaaaaatg gagcccagct
    18181 tccctatgaa gaccatccga ttgtttccaa tctcttcttt gaggattcta cacggacaca
    18241 taagtccttt gaagtcgcag agattaaact tggattggaa cgacttgact ttgatgtgaa
    18301 gactagttcg gttaataagg gtgagacact ttatgacacc attttgactc tgtctgcttt
    18361 aggagtggat gtctgtgtga ttcgccatcc agaggtcgac tactatagag aattgattgc
    18421 tagtccaacg attacgactt ccatcatcaa tggtggagat ggttcgggcc aacaccctag
    18481 ccagagcttg cttgatttga tgaccattta tgaggaattt ggccactttg agggtcttaa
    18541 agttgctatt gcaggtgact tggaccactc acgcgttgcc aaatccaata tgcagatttt
    18601 gaaacgcttg ggagctgaac tctttttcgc tggacctgag gaatggagaa gtcaagagtt
    18661 tgcagactat ggaaagtttg taaccattga tgaaatcatt gatcaggttg atgtcatgat
    18721 gtttctccgt gtgcaacacg aacgccatga tagtggagct gtattttcaa aagaagacta
    18781 tcatgcccaa catggcttga ctcaagaacg ttacgatcgt ttgaaagaaa cagcaatcct
    18841 catgcaccca gctccaatca atcgtgatgt agaaatagca gaccacttgg ttgaagcacc
    18901 aaaatcacgg attgtccaac aaatgaccaa tggtgtcttt gttcgaatgg caatcttaga
    18961 atccgtacta gcgagtagaa acgccaacta aaacacaaga cgttaaaaga cgccagtgag
    19021 cgtccacgag aggtgaaaat atgacaaaaa gaattctagt attagaagat ggcacagttt
    19081 ttgaaggcaa ggccttcgga gcagatattg atgtaacagg cgaaatcgtc tttaatacag
    19141 ggatgaccgg ctaccaagaa tccattacag accagtctta taatggacaa atcttgacct
    19201 ttacttatcc tttggtagga aattatggta ttaatcgtga tgattatgaa tccattattc
    19261 caacttgtaa gggagtcgtt gtttttgaag aagcgcgtag agctagcaac tggcgcaacc
    19321 aaatgacctt ggatgaattt ttgaaagcca agaaaattcc agggatttca ggaattgata
    19381 cgcgtgctct taccaagatt atccgtaagc atggtactat gcgtgcaacc ttgacccatg
    19441 ttggggacag tatggaccat gtgacggacc agctccaagc aacagtttta ccgacagaca
    19501 atatcaaaca ggtttctact aaaacgtctt atccagctcc aggagttggt ttgagcgtgg
    19561 tgctagtgga ctttggtctc aagcactcaa tcctacgtga actttctaaa cgcaactgta
    19621 acgtgacagt tgttccttat tcgacaacgg ctgaagaaat tctccatctc aatcctgatg
    19681 gagttatgtt gtcaaatggt ccaggtaacc cagaagacgt tccacaagca ctcgacatga
    19741 ttcgcggtgt acaaggaaaa attccaatct ttggtatttg tatgggacac caactctttg
    19801 caatggcaaa cggggctaag acctataaga tgaagtttgg ccaccgtgga ttcaaccatg
    19861 cggtacgtga aatcgcaaca ggacgagtag acttcaccag ccagaaccat ggttatgcag
    19921 tcagccgcga ggatttgcca gagcacttga ttattaccca cgaagaaatc aatgacaagt
    19981 cagtggaagg tgtgcgccac agataccaac ctgctttctc tgttcaatac cacccagatg
    20041 cagctcctgg gccacacgac gctagctacc tatttgacga gtttatcgag atgatggaag
    20101 tttttaaaca atcaaactaa gaatgagtaa aagctcgtcg gagaatttat gtaactgaac
    20161 atggacggaa agctcagaat ttagagaaac caacttggat tgtcaatcct tccttgattt
    20221 cctaaaattc aatcgctttc tagtcgtcct ttgtatctta tttaatgtcg ccctgagagg
    20281 cgacgaatag aaaggagaag atacattgtt cgcggaagtg aacacgccct aagaactgtg
    20341 tgaaaaagat aaacatcgtc ttgacgctga aggcgtctgc gccgagtttc ctattttcac
    20401 tgtgttcttt acgggctttg tatcataaac tatgcctaaa cgtactgata ttcaaaaaat
    20461 tatggtgatt ggttctggtc cgattattat tggtcaggct gctgagtttg actacgctgg
    20521 gactcaggct tgcttgtcgt tgaaagagga aggttatgag gttgttttgg ttaactcaaa
    20581 tcctgcaacc atcatgacgg acaaggaaat tgctgacaag gtttacattg aaccgattac
    20641 acttgagttt gtgacacgta ttcttcgtaa ggaacgtcca gatgccttgc taccaacact
    20701 cggtggtcag acaggtctca acatggccat ggaattgtct aaaaatggta tcttagatga
    20761 gcttggcgtt gaacttctgg gtaccaaatt atctgccatt gaccaagcgg aggaccgtga
    20821 cctctttaaa caattgatgg aagagcttga acaacccatt ccagaatctg aaattgttaa
    20881 cacagtagaa gaagcagttg cctttgcagc gacaattggc tacccagtca tcgttcgtcc
    20941 agcctttacc ctcggtggta ctggtggtgg tatgtgtgcc aacgagaaag aattgcgtga
    21001 aatcactgaa aatggtttga aattgtcacc tgttacccaa tgtttgattg agcgttcgat
    21061 tgccggtttc aaggaaattg aatacgaagt gatgcgtgac tcagctgaca atgccttggt
    21121 tgtttgtaac atggaaaact ttgacccagt tgggattcac acgggggatt ccattgtatt
    21181 tgcccctgcg caaaccatgt cagactatga aaaccaaatg cttcgtgatg cgagcttgag
    21241 cattattcgt gccctcaaga ttgaaggtgg atgtaatgtt cagctagctc ttgatcctaa
    21301 cagcttcaag tattatgtta tcgaagtaaa ccctcgtgta tcacgttcgt cagcccttgc
    21361 ttctaaagcg acaggatatc cgattgctaa gttggctgcc aagattgcag taggtttgac
    21421 cttggatgag gttataaacc cagttacagg ttcaacctat gccatgtttg aaccagccct
    21481 tgactacgta gttgctaaga ttccacgttt cccatttgac aagtttgaaa agggtgaacg
    21541 ccgtctcggt acacaaatga aggcgactgg agaagtcatg gcaatcggtc gtaacatcga
    21601 ggaatctctt ctcaaggcct gccgctccct tgaaattggg gtgcaccaca atgaaatacc
    21661 tgaacttgca gcagtttctg atgatgcctt gattgaaaag gttgtcaaag cccaagatga
    21721 ccgtctcttc tacgtatcag aagcaattcg tcgtggctac acacctgaag aaattgcaga
    21781 gcttacaaaa attgatatct tctatcttga taaactcttg catatctttg aaattgagca
    21841 agaattgggt gctcatccac aagatctaga agttttgaaa acagccaaac ttaatggttt
    21901 ttcagaccgt aagattgctg aactctgggg aacgactgat gatcaagttc gccaacttcg
    21961 cttggaaaac aagattgttc cagtttataa gatggtagac acttgtgcgg cagagttcga
    22021 ctccgaaacg ccatatttct attcaaccta tggttgggaa aatgaatcta tcagatctga
    22081 taaagaatct gtacttgtcc taggttcagg tccaatccgt attggtcaag gggttgagtt
    22141 tgactacgca accgttcact ctgttaaggc tatccaggca gctggttatg aagctatcat
    22201 catgaactca aacccagaga ccgtttcaac agacttctct gtatcagata agctttactt
    22261 tgagccattg acattcgaag atgttatgaa cgtcattgac ttggaacaac caaagggagt
    22321 tatcgttcag ttcggtggtc aaacagccat caaccttgcg gagccattgg caaaagcagg
    22381 tgtgaccatc cttggtacac aggttgctga cctagatcgt gccgaagacc gtgacctctt
    22441 cgagcaagct ctcaaagaat tggatattcc acagccacca ggacaaacag ctaccaatga
    22501 agaagaagca gctcttgcag ctcgcaagat tggcttccca gttcttgtcc gcccatctta
    22561 tgtacttggt ggacgtgcca tggaaatcgt tgaaaacgaa gaagaccttc gttcttacat
    22621 gcgaactgct gttaaggcta gtccagacca cccagttctt gtcgactctt atatcgttgg
    22681 gcaagagtgc gaagttgatg ccatttcaga tggtaaaaat gtactcattc ctggtatcat
    22741 ggagcatatc gaacgtgctg gtgtccactc aggtgactca atggctgttt acccaccaca
    22801 aaccttgtcg caaaaggttc aggagaccat cgcagactac accaaacgct tagcaatcgg
    22861 tcttcactgc cttggaatga tgaacatcca gtttgttatc aaggatgaaa aagtctacgt
    22921 tattgaggtc aatccacgtg ccagccgtac tgttccattc ctatctaagg taaccaatat
    22981 tcctatggct caagtagcga ccaaactcat tcttggtcaa agtttatcag aacttggcta
    23041 ccaaaatgga ctttaccctg aaagcacccg tgttcatatc aaggcacctg tcttctcctt
    23101 taccaaacta gctaaggtag acagcttgct cggtcctgaa atgaagtcaa caggtgaagt
    23161 tatgggttct gatgctactt tggaaaaagc tctctataaa gcctttgaag cttcttatct
    23221 acacttgcca acttttggta atgtcgtctt taccatcgct gatgatgcca aagaagaagc
    23281 cttgaacttg gctcgtcgtt tccaaaacat tggttatgga atccttgcga cagaagggac
    23341 agcagccttc tttgctagcc atggcttgca agcccaacct gttggtaaga ttggtgatga
    23401 cgataaggat atcccaagtt ttgtacgcaa aggaagaatt caagctatca ttaacacagt
    23461 aggaacaaaa cgaactgctg acgaagatgg tgagcaaatt cgtcgttcag ccattgaaca
    23521 cggggtgccc ctcttcacag cgctagatac agctaatgcc atgcttaagg tacttgaaag
    23581 tcgtagcttt gttacagaag cgatttaaat agaaaaataa gatatagcag actgaattta
    23641 gaacagtaca caggaatact aaaatatttc tagaaattaa tttgaatttt ctaattgatt
    23701 tgttcgcatc ttatttcaat ctactataga aaaaagtctt taaaattata aaacgcatca
    23761 tatcaaggtt tttcaaaaac cttgatatga tgcgttttat tgtgggaata tgtatttcat
    23821 tttctactaa aattatgttt ttgaataacc tctatcttag tagtttgtat aatccccctc
    23881 aatcagtttt tgcgataagc tttaatgcta tgactatacc actcttgcat ttcttttgat
    23941 ggtggtgtca tataatcgcc atacatctgg gttaaaaatt ggtcatattt tttgggaaca
    24001 ggcaacatac ggccctcaaa ctcagttaaa atcagttctt taaaggtatc aactgggaag
    24061 atttctttca tcccttcctt accgatccca actcctcctt catattgagg aatgttggtt
    24121 acagcatttt tgactagttg atcaattttc ttgtaaaagt agcgaggatt gacaaatcgg
    24181 agagcgtacc agctacataa tctaagaaaa tcttttagtt tgctatcacc gtgaactgct
    24241 cgtgattttt tgatgtaagc tagttgacga agggccacat acttatagct cttgtcgaca
    24301 atgctcaagt ctgtaaattg atcaattggg aagacatcga tgaaaaggct ggtatcatga
    24361 cgcttgtact taacatggtc ttctataaca gtagaagtat ccaaaatcga tgcgaaatta
    24421 tggaagtacc aagaagatgt atcgtaggaa agaaccttgt agcgagggtg attttcttct
    24481 tcaataatct tcagtaaacg ctcataatcc tcacgataaa gggaaatatc aatatcatca
    24541 tcccaaggaa tcataccttt gtggcggatg gctccaagca tggttccata actgagaaaa
    24601 taaggaatat catgtttctt acaagtctca tcaatatagt ccagcagggc tagttgaatt
    24661 tctttaattt cttttttttc taaatattgc atcctaatcc tccaatttat aagcgtgaaa
    24721 ttcatgactg tagaagcgtt tttcttctgg tggtagggtc atataatctc cataaaattg
    24781 tgtcaaaata gtatcaaatt tttcaggtgc aggaaggctt aaattctcaa agggtaaatc
    24841 gattgtttta tcaaaggtag cacttgggaa gacttccttt tccttaaatt ttgaaggaat
    24901 aaaagccata tactgcccat tttcacgact atatttttga atttctttct cgattttatt
    24961 tgcaaaataa cgaggagaaa ccagtcgaag gagcaaccaa aaggctgttc gtatccaatc
    25021 ttttaaaagg ctatccttat agacaatatt tttatgttta ctgaaagaca gcagtttgaa
    25081 gctttctagt ttataacaag tatcaatgac cttaggatca tcaaagcgat ccatagggaa
    25141 aatatcgata aagataccag actcataggt ttttgtattt cgagtatcaa taattttagt
    25201 cgtactgtcg gttatcttga taaagttgtt aaagtagttc ttatcagttt ctaaggatag
    25261 gagcttatac ttgctttttt ccttttgaaa aatgttaata aatcgttggt agtcttctct
    25321 aggcatggat aaatcaatat cgtcgtccca agggataaag ccctcatgtc gaaccgcccc
    25381 aatcagagta ccgtagttaa taatatagtt gatattgtgc tttttacaga gagtatcaat
    25441 ataattcaaa atttctaatt caatttgttt ggcatcttca acggttagtt gtttcatttt
    25501 aaactcctaa gattttttaa atttattttt taaggctagg acatggttta aaaattcata
    25561 gaaaatgcta tcttttgtga agacaagtag actaatataa gagatagctg atagcaagac
    25621 aatcaaacca gtattaatca aaaatggcaa attaatgacc atatccacag gatacacgaa
    25681 attaatcagg aaataaattg ctacaaagga aagtgaaaag agagagtatc gaacagtata
    25741 gctaaagata tgtcccaagt ggatgagttg ttttctatgg atgaaaatga tatagaaaac
    25801 aagtagagaa gcctctgata acatagttgt cagtaagtag tattcgggag ccacgatatg
    25861 gttgaaaaag aggagactat tcaagcccaa attgagtagc ccagcaaaga ctgtatagac
    25921 tgtgatacgt ttttcatagc cattggtaaa gagaatttgg gaaccaagaa tggtatctaa
    25981 ggccaggata atcgtacgaa aagcgaagag agaggtcaag atgtcgcctc cgatatattt
    26041 ttcactaccg taaagtagga tggcatttgg tcctaaaacc atgagtccaa aactcagtgg
    26101 aatgataaag aagttaaaga ttcgactacc tctattaacc agagaaacat aggcttcttt
    26161 gtctcctttc cccagatagt aactgagacg aggcacactc actccaattg cacctgttac
    26221 aaccccagct ataacggtca caattcgctg agctatggta tagtaactaa cgttgacatc
    26281 aatccctgtt ttaacgagga agaggcgatc taaaaaagtg aagagcatat tggcattggc
    26341 aaagactaac atggctgtca gagggagaaa gagtggttta aaatcactta ggtgaatctt
    26401 aacaagtttg atgtctcttt taatccaaaa ataactaatc aggtagttaa tcagcgtcga
    26461 taaactcatc acaagtgtat agacaacaat atcgtgttca tttttaacaa ataagaaaat
    26521 agagaccagc atcaggatac ggatgaaggc agttttgtaa aagagaaaac tgtaattttc
    26581 cagagcttca ttgacccatt cgattgaaaa aatctgggca atgagttgaa tccccataac
    26641 aaggtagacc tttttgacga ttggattatc agtaaagaag agaggatagg ctaggatata
    26701 gacagcagtg gtcaaaatcg tacaagcgat gcacaaataa aaaagactag aaaaggttct
    26761 gttaagatct tttttgttat ccttgacatt actgatagcc cttaaaccgt agttatagac
    26821 accataagtt gcaaagggca agaaaaatga caaaatagtg tcgactgagt tgaagtaacc
    26881 atagtcagtt cggtccaaga cacgcgcgac ataggttcca gttaggatgg gaaaaataat
    26941 attcaagaca cgaattccca tgtaagatag agcatttaat tttatacttt tcattcaatt
    27001 tacctcgttt ttcattatat cataaagtta gctaataaga aatgaagggc agtaagtcaa
    27061 gtaatcactt tgaagtttca aatcttaagt tttaagtttt ctttaaggaa agtatattat
    27121 tctgaaggac tctaaaattt cgcagccatt tattagtaat tgctacagaa ttcctagtca
    27181 ttactagaaa tggactagtt tctttgaata atagaactgc ataattctcc tattctagaa
    27241 ggggaggacc agtatttctt ttatgatagg actagattgt ggtataatag agagaataag
    27301 tttttttagt aagacaaagg agaaaataga tgatttatgc aggaattctt gccggtggaa
    27361 ctggcacacg catggggatc agtaacttgc caaaacaatt tttagagcta ggtgatcgac
    27421 ctattttgat tcatacaatt gaaaaatttg tcttggagcc aagtattgaa aaaattgtag
    27481 ttggtgttca tggagactgg gtttctcatg cagaagatct tgtagataaa tatcttcctc
    27541 tttataagga acgtatcatc attacaaagg gtggtgctga ccgcaataca agtattaaga
    27601 aaatcattga agccattgat gcttatcgtc cgcttactcc agaggatatc gttgttaccc
    27661 acgattctgt tcgtccattt attacacttc gcatgattca ggacagtatc caacttgccc
    27721 aaaatcatga cgcagtggac acagtggtag aagcggttga tactatcgtt gaaagtacca
    27781 atggtcaatt tattacagat attccaaatc gtgctcacct ttatcaagga caaacacctc
    27841 aaacattccg ttgcaaggac ttcatggacc tttatggatc tctttctgat gaagagaagg
    27901 aaatcttgac agatgcatgt aaaatctttg tgatcaaagg aaaagatgtg gccttggcca
    27961 aaggtgaata ctcaaatctg aagattacaa ccgtaacaga tttgaagatt gcaaaaagta
    28021 tgattgagaa agactagtaa aatgattaat caaatttatc aactaactaa gcctaagttt
    28081 atcaatgtca aatatcagga agaggctatt gaccaagaga atcatatcct tatccgtccc
    28141 aactacatgg ctgtctgtca tgcggatcag cgttactatc agggaaaacg tgatcccaag
    28201 attttgaata aaaagcttcc aatggcaatg attcacgagt catgtggaac cgtcatttct
    28261 gacccgaccg gaacctacga ggttggtcaa aaagttgtca tgattcccaa tcagtctcct
    28321 atgcagagtg atgaagaatt ctatgaaaac tacatgacag ggacccattt cttgtctagt
    28381 ggatttgatg gctttatgag agagtttgtt tctctcccta aagatcgtgt ggtggcttat
    28441 gatgctattg aagatacggt tgcagccatt acagagtttg tcagtgtggg catgcacgct
    28501 atgaatcgtc tattgactct tgctcatagc aagcgggagc ggatcgccgt tattggagat
    28561 ggaagtttag cttttgtggt tgccaatatt atcaactata ctttgccaga agcagagatt
    28621 gtggttattg gtcgtcattg ggaaaagttg gaactcttct catttgccaa agaatgctat
    28681 attacggata atattcctga agagttggcc tttgaccatg cttttgaatg ttgtggtggt
    28741 gatggtactg gaccagctat taatgacttg attcgctaca ttcgtcctca gggaacgatt
    28801 ctcatgatgg gagttagcga atataaagtc aatctcaata ctcgcgatgc cttagaaaag
    28861 ggcttgattt tggttgggtc atctcgttct ggtcgcattg attttaaaaa tgctatccaa
    28921 atgatggaag tcaagaaatt tgccaatcgt cttaaaaata tcctttatct agaagaacct
    28981 gtaagagaaa ttaaagatat tcatcgtgtc tttgcaaccg atttaaacac agcctttaaa
    29041 acagtgttta agtgggaagt ataagtactg gaggttaatt gtggagaaaa tcattaaaga
    29101 aaaaatttct tccttactta gtcaagaaga ggaagtcctc agtgttgaac aactgggtgg
    29161 aatgaccaat caaaactatt tggccaaaac aacaaataag caatacattg ttaaattctt
    29221 tggtaaaggg acagaaaagc ttatcaatcg acaagatgaa aagtacaatc ttgaactact
    29281 aaaggattta ggcttagatg taaaaaatta tctttttgat attgaagctg gtatcaaagt
    29341 aaatgagtat atcgaatctg cgattacgct tgattcaacg tcaatcaaga ccaagttcga
    29401 caaaattgct ccaatattac aaactattca tacgtctgct aaggaattaa gaggagaatt
    29461 tgctcctttt gaagaaatca aaaaatacga atccttgatt gaagaacaaa ttccttatgc
    29521 caactatgaa tctgttagaa atgcagtctt ctccttagag aaaagactgg ctgacttagg
    29581 tgttgacaga aaatcttgtc atatcgattt ggtgcctgaa aactttatcg aatcacctca
    29641 aggacgactt tatttgattg actgggaata ttcatcaatg aatgatccaa tgtgggattt
    29701 ggctgccctc tttttagagt ctgaattcac ttcccaagag gaagaaactt tcttatctca
    29761 ctatgagagt gaccaaacac cggtttctca tgaaaagatt gctatttata aaattttaca
    29821 agatactatt tggagtctat ggactgtcta taaggaagag caaggtgaag attttggtga
    29881 ctatggtgtg aatcgttacc aaagagctgt taaaggtttg gcttcttatg gaggttcaga
    29941 tgaaaagtaa aaacggagtt ccttttggcc ttctctcagg tattttctgg ggcttgggtc
    30001 taacggttag tgcttatatc ttttcgattt ttacagattt gtcacccttt gtggtggctg
    30061 cagctcatga ttttttgagc atctttatct tactagcttt tctcttggta aaagaaggga
    30121 aagttcgcct ctcaattttc ttaaatattc gcaatgtcag tgttatcatc ggagccttgc
    30181 tagcaggccc tatcggtatg caggccaatc tttatgcagt taagtatatc ggaagttctt
    30241 tagcttcatc tgtatcggct atttaccctg cgatttcagt tctattggct ttcttctttt
    30301 tgaagcacaa gatttcgaaa aatactgtat ttgggattgt cttgattatt ggagggatta
    30361 ttgcccagac ctataaagtt gaacaggtta attctttcta cattgggatt ctttgtgctt
    30421 tggtttgtgc tattgcatgg ggaagtgaga gtgttcttag ctcttttgcc atggaaagtg
    30481 aattgagtga aatcgaagcc ctcttaatcc gtcaagtaac ttcgttcttg tcctatcttg
    30541 tgattgtgct cttctctcat cagtcattta ctgcagtagc caatggacaa ttgctaggtc
    30601 tcatgattgt ttttgcagcc tttgatatga tttcctactt ggcttattat atcgctatca
    30661 atcgcttgca accagccaag gctacaggct tgaacgtgag ctatgtagta tggacggtct
    30721 tgtttgcagt tgttttcttg ggtgcaccgc tagatatgct gaccattatg acgtcacttg
    30781 tcgtcattgc tggagtttat attattatta aagaataaag gagattcgtg tgaaagccat
    30841 catcttagca gcgggattgg gaactcgctt gcgtcctatg actgaaaata cccctaaagc
    30901 cttggttcag gttaatcaaa aacctttgat tgagtaccaa attgagtttc tcaaagaaaa
    30961 aggaatcaat gacatcatca tcattgttgg ttatcttaaa gaacaattcg attacttgaa
    31021 agagaaatac ggtgttcgtc tcgttttcaa tgataaatac gctgactaca ataactttta
    31081 ctctctctat cttgtaaaag aagaattagc caatagttat gttatcgatg ccgataacta
    31141 tctctttaaa aatatgttcc gcaatgattt gacacgttct acttatttta gtgtctatcg
    31201 tgaagattgt accaacgaat ggttcttggt ctatggagat gactacaagg ttcaaaacat
    31261 tattgttgat agcaaggcag gtcgcatcct tagtggtgta tccttctggg atgctccaac
    31321 agcagaaaaa attgttagct ttatcgataa ggcttatgca agcggcgaat ttgttgatct
    31381 ctattgggac aatatggtta aggataacat caaagagcta gatgtctatg ttgaagaatt
    31441 agaaggcaat agcatttatg agattgatag tgtccaagac tatcataaat tagaagaaat
    31501 tcttaaaaac gaaaattaaa agcttccaac atctgacaaa atagtcggat gtttttttga
    31561 ttttttacga acttttacga atagatagat gagtagaaaa agaaatggag ttatttatga
    31621 aaatcacaaa ctatgaaatc tataagttaa aaaaatcagg tttgaccaat caacagattt
    31681 tgaaagttct agaatacggt gaaaatgttg atcaggagct tttgttgggt gatattgcag
    31741 atatatcagg ttgccgtaat ccagccgttt ttatggaacg ttattttcag atagacgatg
    31801 cgcatttgtc gaaagagttt caaaaatttc catctttctc tattttagat gactgttatc
    31861 cttgggattt gagtgaaata tatgatgcgc ctgtactttt attttacaag ggaaatcttg
    31921 acctcctgaa attcccgaag gtagcggtcg tgggcagtcg tgcttgtagc aaacagggag
    31981 ctaaatcagt tgaaaaagtc attcaaggct tggaaaatga actggttatt gtcagtggtc
    32041 tggccaaggg cattgacaca gcagctcata tggcagctct tcagaatggc ggaaaaacca
    32101 ttgcagtgat tggaacagga ctggatgtgt tttatcctaa agccaataaa cgcttgcaag
    32161 actacatcgg caatgaccat ctggttctaa gtgaatatgg acctggcgaa caacctctga
    32221 aatttcattt tcctgcccgt aatcgcatca ttgctggact ttgtcgtggt gtgattgtag
    32281 cagaggctaa gatgcgttca ggtagtctca ttacgtgtga gcgagcaatg gaagaaggac
    32341 gcgatgtctt tgctattcct ggtagcattt tagatggact atcagacggt tgccatcatt
    32401 tgattcaaga aggagcaaaa ttggtcacca gtgggcaaga tgttcttgcg gaatttgaat
    32461 tttaaaaatg acctaagcta gaattctaag aaaaaatcaa ttttaagaga aaatgaaccc
    32521 aacatttcca taataaaacg catattagca agtttttaac acttgataat atgcgttttt
    32581 tctaagtgga ttagtagagt agaggatttt tctcatataa tactcttcga aaatctcttc
    32641 aaactacgtc agcttccatc tgcaacctca aaacagtatt ttgagctgac ttcgtcagtc
    32701 ttatctacaa cctcaaagca gtgctttgag caacctgtgg ctagcttcct agtttgcgct
    32761 ttgattttca ttgagtataa gggaaagtat agtgaattga aataagatgt gaacaactct
    32821 atcaggaaag tcaaattaat ttatagaaat attttagcag ccaaggtgca ctgttataga
    32881 ttcaatacac tataatttag tgtaattgag aaaggagaaa tgattgtgat tgatgttggc
    32941 taggttatgt tcaatgattc ctaccgtctc aaatcttgtc agtaaggaaa aataaattct
    33001 tcaaaagtag agattacaag gcttgtttaa gaaagaattc aaagaccttg acaaataaaa
    33061 ataaaatggt tattataaaa aatggtctga aatagatgat gatacttttc gaaaatcttt
    33121 ttacgtcagc tcagctttgc cttgctgtgt tttgagcaag ctacggttag cttccgagtt
    33181 tgattttcat ttactagaaa tgaaactgat gagagatatc agtagacatt tgagtcagga
    33241 tattatggaa aatgataaaa agagctcgtg agattggcat atcagactac taaagtattg
    33301 agtttgttag gattttagcg actagttagc tgggaaagga agatatttgt gacaaataat
    33361 aaactgtatt cgttgataga atttagaaat aaaatatatg aagaattaga actttccaga
    33421 agtgatttag cgattttact atgtgccatg cttatcgcct ctatcggatt aaatatggat
    33481 tcgactcccg tgattattgg agccatgtta atctctcctt tgatgacacc tattctggga
    33541 gtggggctct ctctagctat atttgatttt aaattgttaa gaaaatcttt taaaatatta
    33601 gctattcaaa ttcttgccag tttaatagct tcaacacttt atttttatct ttctcccatt
    33661 tcgtatgcta gttcggaaat tgttgctaga acctctccga ctatttggga tgttctcatt
    33721 gcttttgtag gagggatagc aggtattatt ggtgctagga aaaaagagac caataatatt
    33781 gttcctggtg ttgctattgc aaccgccttg atgcctcctc tttgtacagt aggttatgct
    33841 attgcttctg ctaatctaaa atttatcata ggctcctctt acctattcct catcaattgt
    33901 agctttattg tcattgcgac ttatataggt gttaggttga tgatggttaa gaaacattat
    33961 tttaaagata atgaagaaga ctctaaaatg cgtaggattt tgcttctagt tgctgttttg
    34021 ctgatgattc cgagtttcat ctctgcaacg actttagtga gagaaacgtt gaaaaaagag
    34081 tcccttaaga aatttatatc agagcagttt caggggcata atattttgaa aaaaacctat
    34141 tctaaaaaga ctcataccct aaagctaacc atttcaggaa attatttgac agaagaagaa
    34201 ctcgatatga tttcaagtaa gagaggtgac tatggtttaa gtgatgtttc tgttcaagtt
    34261 tcacaattgt ctgattcaga acaacttagt aaggaagaac tggtggagta tttcttccag
    34321 tatatcaagg ataaggaagc aaaagaaaag gaaaaagcta ataagtttta tacagagtct
    34381 gaggagcaat aatttcttga gaatagctgg tttctctcgt gagtcttcta tgtatatcaa
    34441 aggaagactg aggttttaag tataaaactt ttcttctatt atagtagcta agtcttgaca
    34501 gggttcaaaa aatggtttac actttataaa gtttattact ttgaaaaggt gtgatactgt
    34561 ggctacggca acaaaaaaga aaaaatcaac agttaaaaaa aatctagtca tcgtggagtc
    34621 gcctgctaag gccaagacga ttgaaaaata tctaggcaga aactacaagg ttttagccag
    34681 tgtcgggcat atccgtgatt tgaagaaatc cagtatgtcc gtcgatattg aaaataatta
    34741 tgaaccgcaa tatatcaata tccgaggaaa aggccctctt atcaatgact tgaaaaaaga
    34801 agctaaaaaa gctaataaag tttttctcgc gagtgacccg gaccgtgaag gagaagcgat
    34861 ttcttggcat ttggcccata ttctcaactt ggatgaaaat gatgccaacc gtgtggtctt
    34921 caatgaaatc accaaggatg cagtcaaaaa tgcttttaaa gaacctcgta agatcgatat
    34981 ggacttggtc gatgcccaac aagctcgtcg gatcttggat cgcttggtag ggtattcgat
    35041 ttcgcctatt ttgtggaaga aggtcaagaa gggcttgtca gcaggtcgcg ttcagtccat
    35101 tgcccttaaa ctcatcattg accgtgaaaa tgaaatcaat gccttccagc cagaagaata
    35161 ctggacagtt gatgctgtct ttaaaaaggg aaccaaacaa tttcatgctt ccttctatgg
    35221 agtagatggt aaaaagatga aactgaccag caatgacgaa gtcaaggaag tcttgtctcg
    35281 tctgactagt aaagactttt cagtagatca ggtggataag aaagagcgca agcgcaatgc
    35341 tcctttaccc tataccactt catctatgca gatggatgct gccaataaaa tcaatttccg
    35401 tactcgaaaa accatgatgg ttgcccaaca gctctatgaa ggaattaata ttggttctgg
    35461 tgttcaaggt ttgattacct atatgcgtac cgattcgact cgtatcagtc ctgtagcgca
    35521 aaatgaggcg gcaagcttca ttacggatcg ttttggtagc aagtattcta agcacggtag
    35581 caaggtcaaa aacgcatcag gtgctcagga tgcccatgag gctattcgtc cgtcaagtgt
    35641 ctttaataca ccagaaagca tcgctaagta tctggacaag gatcagctca agctatatac
    35701 ccttatctgg aatcgttttg tggctagcca gatgacagcg gccgtttttg ataccatggc
    35761 tgttaaattg tctcaaaatg gagttcaatt tgctgccaat ggtagtcagg ttaagtttga
    35821 tggttatctt gccatttata atgattctga caagaataag atgttaccgg acatggttgt
    35881 tggagatgtg gtcaaacagg tcaatagcaa accagagcaa catttcaccc aaccgcctgc
    35941 ccgttattct gaagcaacac tgattaaaac cttagaggaa aatggggttg gacgtccatc
    36001 aacctatgcg ccaaccattg aaaccattca gaaacgttat tatgttcgcc tggcagccaa
    36061 acgttttgaa ccgacagagt tgggagaaat tgtcaataag ctcatcgttg aatatttccc
    36121 agatatcgta aacgtgacct tcacagctga aatggaaggt aaactggatg atgtcgaagt
    36181 tggaaaagag cagtggcgac gggtcattga tgctttttac aaaccattct ctaaagaagt
    36241 tgccaaggct gaagaagaaa tggaaaaaat ccagattaag gatgaaccag ctggatttga
    36301 ctgtgaagtg tgtggcagtc caatggtcat taaacttggt cgttttggta aattctacgc
    36361 ttgtagcaat ttcccagatt gccgtcatac ccaagcaatc gtgaaagaga ttggtgttga
    36421 gtgtccaagc tgtcatcagg gacaaattat tgagcgaaaa accaagcgta atcgcctatt
    36481 ctatggttgc aatcgctatc cagaatgtga atttacctct tgggacaagc ctgttggtcg
    36541 tgactgtcca aaatgtggca acttcctcat ggagaaaaaa gtccgtggtg gtggcaagca
    36601 ggttgtttgt agcaaaggcg actacgagga agaaaagatt aaataagagg aggggcccga
    36661 aagtaaattt caggctcctt ttggttagaa cttgacaaaa tacatcattt tatgcgaaac
    36721 tagaagaaga ttattttata ctcaatgaga atcaaagagc aaattagaaa gctagtcgca
    36781 ggttgctcaa agcactactt tgaggttgaa gatggaagct gacctggttt gaagagattt
    36841 tcgaagagta ttaactagga gaagttatgc gtattatcta tctaattatt ggttttttat
    36901 cgctgacctt ggctattgtt ggggttgttt tacccttgtt gcctacaaca cctttccttt
    36961 tgttgtctat tgcttgtttc tccagaagtt ccaagcgatt cgaagattgg ctttatcata
    37021 ccaagctcta tcaagcatat gtagctgatt ttcgtgagac caagtctatt gcgcgtgaac
    37081 gaaagaaaaa aatcatcgtc tctatctacg tcttgatggg aatttctatt tattttgcac
    37141 ctcttttacc agtcaaaatc ggtctgggtg ctttgaccat ctttattact tattatctct
    37201 tcaaggtcat tccagacaaa gaatagttaa aacagtagtt atttgccttg ataaaattga
    37261 aagcatattc ataacaatat gatataataa aattgaagta atattcaagg agaatcaaat
    37321 gatttacgaa ttttgtgctg aaaatgtgac tttacttgaa aaagcgatgc aggctggagc
    37381 tcgtcggatt gaactctgtg ataatctagc agttggtggg acaacaccca gctatggagt
    37441 gactaaggca gcggttgaac tggcagctaa ctacgataca accatcatga ccatgattcg
    37501 gccacgtggt ggtgactttg tctataatga cctagaaatt gctatcatgc tagaagacat
    37561 tcgtttgact gctcaggctg gaagtcaagg ggttgtattt ggagctttaa ctgctgataa
    37621 aaagttggat aagcctaatc tggaaaagtt aattgctgca tcaaaaggaa tggaaattgt
    37681 ctttcacatg gcctttgatg aactaagtga tgaagatcaa ccggaagcta ttgactggct
    37741 cagtcaagcc ggtgtcactc gtatcctaac tcgtgctggt gtgtctggcg actccttaga
    37801 aaaacgtttt gttcactatc acagaatttt ggagtacgct aaaggtaaaa ttgaaattct
    37861 accaggtggg gggattgacc ttgaaaaccg tcaaaccttt atcgaccagg tgggggtaac
    37921 acaattgcat ggtactaagg ttgtttttta aaaaatagaa aggaactgct agctttgggt
    37981 agcagttttc acttatgttt gaaattttta aatcctatca atttaatcaa gaaaaggctc
    38041 atgattatgg ttttatagaa aatagcgaag tctggacata tagttgccag attttgcaag
    38101 gtgactttgt catgactgtg tccatcactg ctgataatgt gaactttcaa gtctttgacc
    38161 aagagactgg tgacctctat cctcacgttt atatggaaag catgagggga agttttgtcg
    38221 gaaatgtccg tgaggcttgt ctggagattc tttaccagat tcggaaggct tgttttgatg
    38281 tgcaagattt tatctgtcat cagactaagc gtatcatgac tcaagttcag gaaaagtatg
    38341 gaaaccagtt ggagtatctg tgggaaaaat cgcctgatac agctgtattg cgccatgaag
    38401 gcaatcaaaa gtggtatgcc gtcttgatga aaatctcttg gaataagctg gaaaagggca
    38461 gagaaggaca agtggaagca gtcaacctca agcatgacca agtagctaat ttgctttcac
    38521 aaaaggggat ttatccagcc ttccatatga gcaagcgcta ctggattagt gtgtcccttg
    38581 atgatacttt atcagatgaa gaagtactgg aattgataga aaaaagttgg aacttaacct
    38641 ctaaaaaatg aaatatttta ataattttca tgaactttca attagctaaa tattctttac
    38701 tgaagagatt tttagaaaat ataggattta ccacactaga ggaatatggt gccatcttca
    38761 aatacctgat tgagaatgtc aagacggatc gtcagatcat ctattcgcct cactgtcatg
    38821 atgacctcgg aatggcagtg gcaaatagcc ttgctgctgt caagaatggt gcaggacgtg
    38881 ttgaagggac tatcaatggt attagggagc gagctgaaaa tgctgctttg gaagaaattg
    38941 cagtggctct caatattcgc caagattact accaagtaga aaccagtatt gtcctaaatg
    39001 agaccatcaa tacgtcagaa atggtttctc gcttctctgg tattccagtt cctaaaaaca
    39061 aagccgtcgt tggtggcaat accttctccc acgaatctgg tattcaccaa gatggagtcc
    39121 ttaaaaatcc tctcacttat gagatcatca cacctgaatt ggttggtgtt aagattctgc
    39181 ttggaaaatt atctggtcgc catgcttttg ttgagaaact gagagaattg gccctagatt
    39241 ttacagaaga ggatatcaaa ccactctttg ctaagttcaa ggcactggtc gataagaagc
    39301 aagaaatcac agatgcagat attcgagctt tggtagctgg aaccatggtt gaaaatccag
    39361 aaggcttcca ctttgatgat ttacaacttc aaactcatgc agataatgac attgaagcgc
    39421 tcgttagcct agccaatatg gatggtgaga aagtcgaatt taatgcgaca gggcaaggtt
    39481 ccgttgaagc aatctttaat gctatcgata agttctttaa ccaatctgtt cgtttggtgt
    39541 cctacactat cgatgcggta acagatggaa tcgataccca ggatcgggtt ttggtcactg
    39601 ttgaaaacag agatacagaa accatcttta atgcagcagg gcttgatttt gatgtgttga
    39661 aggcttctgc tattgtctat ataaacgcta atacctttgt tcaaaaagag aatgcaggtg
    39721 agatgggacg cagtgtttct taccacgata tgcctagtgt gtaaaggaga aggctatggc
    39781 aaagaaaata gtagctctag caggagacgg aattggccca gaaatcatgg aggttggttt
    39841 agaagttctg gaggctctag ctgaaaaaac aggttttgac tatgagattg acagacgacc
    39901 gttcggaggt gcagatattg atgcagcatg acctccctta cctgatgaaa cccttaaggc
    39961 aagtagggaa gcagatgcta tcctactagt agctatcggt agtcctcagt atgatggagc
    40021 agtggttcgc cctgaacaag gcctgatggc tctccgtaag gaactcaatc tttacgctaa
    40081 tattcgtcct gtaaaaatct ttgacagtct caagcatttg tcactactca aactggaacg
    40141 aattgctggt gtagactttg tcgtggtgcg tgaattgaca ggcgggattt actttggaga
    40201 tcatattctt gaagagcgca atgcgcgtga tatcaacgac tatagctatg aggaagtgga
    40261 gcggattatt cgcaaagcct ttgaaattgc aagaaatcgc agaaaaatcg ttactagtat
    40321 cgataagcaa aatgttctag cgacctcaaa actctggcgg aaagtagctg aggaagtcgc
    40381 acaggatttc ccagatgtaa ccttggaaca tcagctggta gactcagctg ctatgcttat
    40441 gattaccaat cctgctaagt ttgatgttat tgtaacggag aatctttttg gagatatttt
    40501 atctgatgaa tcaagcgtct tatctggtac acttggggtt atgccatcag ccagtcattc
    40561 tgaaaatgga ccaagtctct atgaacctat tcacagttca gcacctgata ttgcaggtca
    40621 aggaattgcc aatcctattt ccatgatttt atcagtttcc atgatgttga gagatagttt
    40681 cggacgttat gaggatgcag agcgtatcaa acgtgctgtt gagacaagtc tggcggcagg
    40741 aattttaacg agagatatag gaggtcaggc ttcaacaaag gaaatgacgg aagctattat
    40801 tgcaaggtta tgaagttaga cgaaaaaatt actctagtcc ttttgatttg gaatgtcatc
    40861 attttcttga tttatggtat tgacaaatct aaggcaagga gaagagtttg gcgcatccct
    40921 gagaaaatct tacttatttt agcctttact tttggtggtt ttggtgcctg gctagcagga
    40981 atcatctttc accacaagac tcgaaaatgg tactttaaaa tagtttggtt tcttgggatg
    41041 gtgaccacac tagtagcctt atattttatt tggaggtaat ggatggcagg gtcttcgagg
    41101 gaatacgctg cttgggctct agcggactat ggttttaagg tcgtgattgc aggatctttc
    41161 ggtgacattc attacaataa tgaactcaat aatggcatgt tgccaatcgt tcagcctaga
    41221 gaggttagag agaaactagc ccagctaaaa ccaaccgacc aggtaactgt ggacttggaa
    41281 caacaaaaaa tcatctcacc agttgaagaa ttcaccttcg agatagatag cgagtggaaa
    41341 cataaactcc taaatagttt ggatgatatc ggtattacct tgcagtatga agagttgatt
    41401 gctgcttatg aaaaacaacg accagcctac tggcaggatt agaaaaaata gaaaaggaga
    41461 tatagtaaac tgaaataaga tgtaaacaaa tgaattggag cttaacatcc atttccagca
    41521 attttttaga aactacagtg gactattctg gattcaacac attataaaat tatgacaaaa
    41581 cacattcaca agaaggctac gacattttaa aaggtgaggg cggatgtatc gtttgcccta
    41641 ctaaagttgg ttacattatc atgaccagtg acaaggcagg acttgagcgt aagttcgcag
    41701 ccaaagaacg taagcgtaac aaaccaggtg ttgttctctg cggtagcatg gatgaacttt
    41761 gcgctttagc gcaactcaac ccagaaattg aagcattcta ctaaaaacat tgggatgaag
    41821 atattcttct tggttgtatc cttccttgga aaccagaagc ctttgaaaaa ctcaaagcat
    41881 acggggatgg ccgtgaagaa cttatgactg atgtacgtgg tactagctgt tttgttatca
    41941 agtttggaaa agcaggtgaa caattggctg ccaagctttg ggaagaaggt aaaatggtct
    42001 acgcctcatc tgcttcaatg acaaaacgat tgaaactcgc tatgagcaag gtgtaatggt
    42061 gtctatggtc gataaggacg gcaaactcat cccagaacaa ggaggagcac gttcaacttc
    42121 accagctcca gttgtgatcc gtaaagggct tgacattgat atagtaaaat gaagtaagag
    42181 caggacaaat taatcaggac agtcaaatcg atttctaaca atgttttaga agtagaggtg
    42241 tactattcta gtttcaatcc actataaaat catgatgcac ctgtcagata cttttaactc
    42301 atgggactac cgtcaggttg agtattatta ggatagagaa gaagtctagt gttaaggaca
    42361 ttacagctca taatactagg cttttttata gaaattcttt tctttttcta taggatatgg
    42421 tattctatgt agaaaatata tgttaataag taatgccaat atttaaacat cattagtaaa
    42481 aggagttaga ttgatgaata aaagaaaagt tagtttagaa gatttttata aatggtatag
    42541 tctaaataaa gaagagttat taaataaggc aactgttggt gaaaagttta atgataaatt
    42601 aaaagaagag tttctccagg aatggccttt ggataggatt ttaacaatgt caatcgatga
    42661 atatgtaata ggaaagggac agcaaaataa gtctttatgc tacgctcttg agaagggaaa
    42721 atacaaaaat ctatttcttg gaatttctgg tggctcagct tcaaaatttg gtatttattg
    42781 gaataaaaaa acaaacaaat ataaagatca agctaataat gagatttcag agttggatca
    42841 gcgattttca aaattaaaat cagatttgta tgaaattatc aaagaaggta ttcgttttaa
    42901 ctttgaaaat cctatttttg atatgaaaag atcaacaaat gaatttattg gtcgttctgc
    42961 tatggtgaca aaattacttt gtatctatac tgagggagat cctttctttg gtgtaaatat
    43021 taatagtcag aaagaatttt ggaaccactt tgtttctcag acaaatcaag gtggacctta
    43081 tctgcaaaat cataaaataa ttgaactgat gtccaaaact tatcctgagt tggagccatc
    43141 gaaattagga actatgcttt ttgagtattc taagcttttt atggaaaata aggaagacaa
    43201 tagtacaatg gattcatcaa acaattttcg tcatcaatta actcaatctc tattaaagtc
    43261 tccaaacctt atccttcgtg gtgctcctgg cacaggaaag acttatcttg ctaaagaaat
    43321 tgccaaagaa ttaacggatg gcaacgaaga tcaaatcgga tttgtacaat ttcacccatc
    43381 atatgattat acggattttg tagaaggttt aagaccagta tcaaatgggg atggagctat
    43441 tgagtttagg ctacaggacg gtatttttaa agatttttgt cagaaagcaa aagaaaccca
    43501 attgattgga ggacaagata attttgatga ggcttgggat tcttacttag aatatataaa
    43561 tgttgctgaa gaaaaagaat atataacaaa aacatcttac ttatctgtta atagtagaca
    43621 aaatttgtca gtaaattatg atagtggtgt tccaggatgg tcactaccta g
//
LOCUS       DAVBBS010000010        41910 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVBBS010000010 DAVBBS010000000
VERSION     DAVBBS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41910
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.010"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            1..537
                     /locus_tag="VQU32_000543"
                     /pseudo
     CDS             1..537
                     /locus_tag="VQU32_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(582..1223)
                     /gene="plsY"
                     /locus_tag="VQU32_000544"
     CDS             complement(582..1223)
                     /gene="plsY"
                     /locus_tag="VQU32_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET2472050.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            1359..3302
                     /gene="parE"
                     /locus_tag="VQU32_000545"
     CDS             1359..3302
                     /gene="parE"
                     /locus_tag="VQU32_000545"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HET2472051.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            3722..6193
                     /gene="parC"
                     /locus_tag="VQU32_000546"
     CDS             3722..6193
                     /gene="parC"
                     /locus_tag="VQU32_000546"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HET2472052.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            6325..7347
                     /locus_tag="VQU32_000547"
     CDS             6325..7347
                     /locus_tag="VQU32_000547"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HET2472053.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            7403..8107
                     /locus_tag="VQU32_000548"
     CDS             7403..8107
                     /locus_tag="VQU32_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2472054.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSDM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGFPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            8172..8861
                     /locus_tag="VQU32_000549"
     CDS             8172..8861
                     /locus_tag="VQU32_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HET2472055.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            8858..9781
                     /locus_tag="VQU32_000550"
     CDS             8858..9781
                     /locus_tag="VQU32_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HET2472056.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            9796..10440
                     /gene="pcp"
                     /locus_tag="VQU32_000551"
     CDS             9796..10440
                     /gene="pcp"
                     /locus_tag="VQU32_000551"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET2472057.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            10608..10838
                     /locus_tag="VQU32_000552"
     CDS             10608..10838
                     /locus_tag="VQU32_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HET2472058.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            10888..10968
                     /locus_tag="VQU32_000553"
     tRNA            10888..10968
                     /locus_tag="VQU32_000553"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10922..10924,aa:Tyr,seq:gta)
     gene            10978..11049
                     /locus_tag="VQU32_000554"
     tRNA            10978..11049
                     /locus_tag="VQU32_000554"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11010..11012,aa:Gln,seq:ttg)
     gene            11211..12413
                     /gene="rpsA"
                     /locus_tag="VQU32_000555"
     CDS             11211..12413
                     /gene="rpsA"
                     /locus_tag="VQU32_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET2472059.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            12499..12996
                     /locus_tag="VQU32_000556"
     CDS             12499..12996
                     /locus_tag="VQU32_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET2472060.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            12996..14651
                     /gene="dnaX"
                     /locus_tag="VQU32_000557"
     CDS             12996..14651
                     /gene="dnaX"
                     /locus_tag="VQU32_000557"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET2472061.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            14679..14873
                     /locus_tag="VQU32_000558"
     CDS             14679..14873
                     /locus_tag="VQU32_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HET2472062.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            15023..15793
                     /gene="sufC"
                     /locus_tag="VQU32_000559"
     CDS             15023..15793
                     /gene="sufC"
                     /locus_tag="VQU32_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET2472063.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITRLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            15821..17083
                     /gene="sufD"
                     /locus_tag="VQU32_000560"
     CDS             15821..17083
                     /gene="sufD"
                     /locus_tag="VQU32_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET2472064.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKINYLERLESRGEGSAKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSYADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            17094..18320
                     /locus_tag="VQU32_000561"
     CDS             17094..18320
                     /locus_tag="VQU32_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2472065.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPASARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            18307..18747
                     /locus_tag="VQU32_000562"
     CDS             18307..18747
                     /locus_tag="VQU32_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET2472066.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            18797..20209
                     /gene="sufB"
                     /locus_tag="VQU32_000563"
     CDS             18797..20209
                     /gene="sufB"
                     /locus_tag="VQU32_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET2472067.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(20440..21681)
                     /locus_tag="VQU32_000564"
     CDS             complement(20440..21681)
                     /locus_tag="VQU32_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HET2472068.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITAYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFIDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            21784..22893
                     /locus_tag="VQU32_000565"
     CDS             21784..22893
                     /locus_tag="VQU32_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET2472069.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            23427..23774
                     /gene="ssrA"
                     /locus_tag="VQU32_000566"
     tmRNA           23427..23774
                     /gene="ssrA"
                     /locus_tag="VQU32_000566"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(23957..24044)
                     /locus_tag="VQU32_000567"
     tRNA            complement(23957..24044)
                     /locus_tag="VQU32_000567"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24008..24010),aa:Ser,seq:gga)
     gene            24206..24946
                     /locus_tag="VQU32_000568"
     CDS             24206..24946
                     /locus_tag="VQU32_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2472070.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNITVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            24943..25854
                     /gene="pfkB"
                     /locus_tag="VQU32_000569"
     CDS             24943..25854
                     /gene="pfkB"
                     /locus_tag="VQU32_000569"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HET2472071.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDVVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            25851..27803
                     /locus_tag="VQU32_000570"
     CDS             25851..27803
                     /locus_tag="VQU32_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2472072.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            27967..30192
                     /locus_tag="VQU32_000571"
     CDS             27967..30192
                     /locus_tag="VQU32_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET2472073.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(30237..30665)
                     /locus_tag="VQU32_000572"
     CDS             complement(30237..30665)
                     /locus_tag="VQU32_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472074.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
     gene            30803..31945
                     /locus_tag="VQU32_000573"
     CDS             30803..31945
                     /locus_tag="VQU32_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2472075.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWLIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRHDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     NYPDIFVFSDEENFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            31954..33168
                     /gene="thiI"
                     /locus_tag="VQU32_000574"
     CDS             31954..33168
                     /gene="thiI"
                     /locus_tag="VQU32_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HET2472076.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            33303..34127
                     /locus_tag="VQU32_000575"
     CDS             33303..34127
                     /locus_tag="VQU32_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2472077.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPGQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYFECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            34149..34888
                     /locus_tag="VQU32_000576"
                     /pseudo
     CDS             34149..34888
                     /locus_tag="VQU32_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            34901..36067
                     /locus_tag="VQU32_000577"
     CDS             34901..36067
                     /locus_tag="VQU32_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET2472078.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIGRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
     gene            36575..38068
                     /locus_tag="VQU32_000578"
     CDS             36575..38068
                     /locus_tag="VQU32_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HET2472079.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            38081..39127
                     /locus_tag="VQU32_000579"
     CDS             38081..39127
                     /locus_tag="VQU32_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2472080.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     LQLDELNLLVKSRFNEMFGDVILNEKEWKVSKWNEILTIRNGKNQKQVEDADGKFPIY
                     GSGGIMGYAKDWIVKKNSVIIGRKGNINKPILVRENFWNVDTAFGLEPVLEKINSEYL
                     FYFCQLYNFEKLNKAVTIPSLTKSDLLNISIPLPPLALQNEFADFVALVDKSQFACEI
                     AIKVWRNSLKFSII"
     gene            39157..41853
                     /locus_tag="VQU32_000580"
                     /pseudo
     CDS             39157..41853
                     /locus_tag="VQU32_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT        12724 a         7540 c         9281 g        12365 t
ORIGIN      
        1 aagaaggcag agaatctcat tcaaggccaa tttgaaggct ctaaaacaat ggaaaagtgc
       61 tacacagatg tgacagaatt tgccattcca gcaagtactc aaaagcttta cttatcacca
      121 gttttagatg gctttaacag cgaaattatc gcctataatc tttcaacttc gcccaactta
      181 gaacaagtat aaacaatgtt ggaacaggca ttcaaagaga agcactacga gaatacgatt
      241 ctccatagtg accaaggctg gcaatatcaa cacgattctt atcatcagtt cctagagagt
      301 aagggaattc aagcatccat gtcacgtaag ggaaacagcc cagacaatgg tatgatggag
      361 tccttctttg ggattctgaa atcggaaatg ttttacggtt atgagaagtc gtttcagtcg
      421 cttaagcaat tggaacaagc cattatagac tatattgatt actacaacaa taaacgaatt
      481 aagataaaac taaaaggact tagccctgtg caatacagaa ctaaatcctt cggataaatt
      541 aattgtctaa cttttggggt gcagttcaca gaactggcat tttatttttt aggatcttga
      601 tgggttaggt tcaatcccca agggaccaaa ttttcagttt tatttttgat acgagctata
      661 ttgtccttat gacgaatgat aatcaaacta gcaagtgcta agataatagc gatgaagaga
      721 gagtcatagt tactcaggat aaaaccaaaa agtggaaaga gcagaacccc gataacagcc
      781 gcgatcgatg ctgtgacact agacagtgaa atcatactgc caagatagag agctccaaag
      841 aagataatcg caaggtagag acagaagata ggcgcaaatc cgaaaattac tccagcactg
      901 gttgcgacag ccttaccacc tttaaatcct gcaaagatag ggaaggtatg gccgataaca
      961 gccaaaagtc caaagatgag aggagaaacg ccttgtagat gaaaaataat cggaagcagc
     1021 gttgctaggg ttcctttgaa aaagtcaatc acaaaggttg ccataccagc tttcttacct
     1081 aaaatgcgga aggtattggt cgttccagtg ttaccagaac catgctcgcg tagattgatt
     1141 tgaaagaata cttgtccaat ccagagacca gatggaatcg aacccagcag ataggctagg
     1201 attaataaaa ctattgtaat catactccta ttatatcatg aattgggaaa gaaaagcaga
     1261 gaatctctgc taaaattgtc acatcggaga aagaaaaatt ttgcaaaatc cttggaaaac
     1321 ctgtagaata gtaaagatga acgaatagga ggttccttgt gtcaaaaaag gaaatcaata
     1381 ttaacaatta taatgatgat gctattcagg tgctagaagg gttggatgcg gtccgaaaac
     1441 gtccagggat gtatattgga tcgaccgatg gcgctggtct tcatcaccta gtttgggaaa
     1501 tcgttgataa tgcagtcgat gaagccttgt ctgggtttgg tgatcgtatc gatgtaacca
     1561 tcaataaaga cggtagtcta acggttcaag accatggacg tgggatgccg acaggtatgc
     1621 acgctatggg aattccaact gttgaggtta tctttaccat tcttcatgcc ggagggaaat
     1681 tcggtcaagg tggctataag acatcaggtg gacttcacgg agtgggttct tccgttgtta
     1741 acgccctttc tagctggtta gaagttgaaa ttacccgtga tggcgcagtt tacaagcaac
     1801 gtttcgaaaa tggtggaaaa cctgtcacga ctttgaagaa aatcggtaca gcacccaagt
     1861 ctaaaacagg caccaaagtt acttttatgc ctgacgcgac tatattttct acgacagatt
     1921 tcaagtacaa taccatttca gagcgcctta atgaatcagc ctttctcttg aaaaatgtga
     1981 ccttgtcttt aacggacaag cgaacagatg aagcgattga gttccactat gagaatggag
     2041 tacaagattt tgtttcttat ctcaacgaag ataaggaaat cttgacgcca gttctttact
     2101 ttgaagggga agacaatggt tttcaagtgg aagtagccct ccagtacaat gacggattct
     2161 cagataacat tctatccttt gtcaataacg ttcgcaccaa ggacggtgga acgcacgaga
     2221 caggactcaa gtctgccatt accaaggtca tgaatgacta tgcacgtaaa acaggtcttc
     2281 tcaaggaaaa agataaaaac cttgaaggtt cagactatcg tgagggacta gcggccgttc
     2341 tttctatctt agttcctgaa gaacacttgc agtttgaagg acagaccaag gataaactag
     2401 gaagccccct agctcgccca gttgtggatg gaatagtggc tgataagttg acctttttcc
     2461 ttatggaaaa tggggaatta gcttctaacc tcatccgcaa ggctatcaag gcccgtgatg
     2521 ctcgtgaagc agcacgtaag gcgcgtgatg agagccgaaa tgggaagaaa aacaagaaag
     2581 ataagggctt gttgtctggg aaattgaccc cagcccaatc taagaatcct gctaagaatg
     2641 aactctatct agttgagggg gactctgccg gtggttctgc caaacaaggt cgtgaccgca
     2701 agttccaggc tattctacct cttcgtggta aggttatcaa tacagccaag gccaagatgg
     2761 cggatatcct caaaaatgaa gagatcaata ccatgattta taccattggt gcgggtgttg
     2821 gagcagactt ctctattgaa gatgccaact atgataagat cattatcatg accgatgcgg
     2881 ataccgacgg tgcccatatc cagaccttgc tcttgacatt tttctaccgt tacatgcgtc
     2941 cgctagtcga ggcaggtcat gtctatattg ccctcccacc tctttacaag atgtccaaag
     3001 gtaaaggaaa gaaagaagaa gtggcctacg cttggacgga cggagaacta gaagaactcc
     3061 gtaaacagtt cggtaaaggc gctaccctcc aacgatacaa aggacttggt gagatgaatg
     3121 cggaccagct ctgggaaaca accatgaacc cagaaacacg taccctcatc cgtgtcacaa
     3181 ttgaagattt agcgcgcgcc gaacgccgcg tcaatgttct catgggagat aaggtagaac
     3241 cacgccgtaa atggattgaa gataatgtca agtttacgct agaagaagcg acagtgtttt
     3301 aatgaaagaa aataatatgt tagtaaactg gaaattagag tcggatgtga atgactatgt
     3361 gaaaaagcag tttgaaaatc ttggtttgaa aaaattgcag gattattaat gaagaatctg
     3421 ctatgagtgc ttatctcaaa gaggcgctta agggagcggc taagacacaa actaagacta
     3481 attttggaaa accagatttt catattgaaa aatacaaaca ggatagtttg gttacttatc
     3541 gtaactgaac acgggaggaa ttcccgtaaa atatctttta ctgcataaaa acctactcta
     3601 cattctttga aaggagttga acacgcccta gatactgtgt gaaaaagata aattctcttg
     3661 tgagtttgct tacttcaaga attttctatt ttcacttggt attttatggg ctttgtatct
     3721 tatgtctaac attcaaaaca tgtccctgga ggacatcatg ggagagcgct ttggtcgcta
     3781 ctccaagtac attattcaag accgggcttt gccagatatt cgtgatgggt tgaagccggt
     3841 tcagcgccgt attctttatt ctatgaataa ggatagcaat acttttgaca agagctaccg
     3901 taagtcggcc aagtcagtcg ggaacatcat ggggaatttc cacccacacg gggattcttc
     3961 tatctatgat gccatggttc gtatgtcaca gaactggaaa aatcgtgaga ttctagttga
     4021 aatgcacggt aataacggtt ctatggacgg agatcctcct gcggctatgc gttatactga
     4081 ggcacgtttg tctgaaattg caggttacct tcttcaggat atcgagaaaa atacagttcc
     4141 ttttgcatgg aactttgacg atacggagaa agaaccaacg gtcttgccag cagcctttcc
     4201 aaacctcttg gtcaatggtt cgactgggat ttcggctggt tatgccacag acattcctcc
     4261 ccataattta gctgaggtca tagatgctgc agtttacatg attgaccacc caactgcaaa
     4321 gattgataaa ctcatggaat tcttgcctgg accagacttc cctacagggg ctattattca
     4381 gggtcgtgat gaaatcaaga aagcttatga gactgggaaa gggcgcgtgg ttgttcgttc
     4441 caagactgaa attgaaaagc taaaaggtgg taaggaacaa atcgttatta ctgagattcc
     4501 ttatgaaatc aataaggcca atctagtcaa gaaaatcgat gatgttcgtg ttaataacaa
     4561 ggtagctggg attgctgagg ttcgtgatga gtctgaccgt gatggtcttc gtatcgctat
     4621 cgaacttaag aaagacgcta atactgagct tgttctcaac tacttattta agtacaccga
     4681 cctacaaatc aactacaact ttaatatggt ggcgattgac aatttcacac ctcgtcaggt
     4741 tgggattgtt ccaatcctgt ctagctacat cgctcaccgt cgagaagtga ttttggcgcg
     4801 ttcacgcttt gacaaagaaa aggctgagaa acgtctccat atcgtcgaag gtttgattcg
     4861 tgtgatttcg attttggatg aagtcattgc tcttatccgt gcttctgaga ataaggcgga
     4921 cgccaaggaa aacctcaaag ttagctatga ttttacggaa gaacaggctg aggctatcgt
     4981 aactttgcaa ctgtaccgtt tgaccaatac cgatgtggtt gtcttgcagg aagaagaagc
     5041 agagcttcgt gagaagattg ctatgctggc ggctattatc ggtgatgaaa ggactatgta
     5101 caatctcatg aagaaagaac ttcgtgaggt caagaagaaa tttgcaactc ctcgtttgag
     5161 ttctttagaa gacactgcga aagcaattga gattgataca gctagtctta tcgctgagga
     5221 agatacctac gtcagcgtga ccaaggcagg ttacatcaag cgtaccagtc cacgttcctt
     5281 tgcggcttcc accttggaag aaattggcaa gcgtgatgat gaccgtttga tttttgttca
     5341 atctgccaag acaacccagc acctcttgat gttcacaagt cttggaaatg tcatctacag
     5401 accaatccat gagttggcag atattcgttg gaaggacatc ggagagcatc tgagccaaac
     5461 catcacaaac tttgaaacga atgaagaaat cctttatgtg gaagtactgg atcagtttga
     5521 cgatgcgaca acctactttg cagtgactcg ccttggtcaa atcaaacggg tagagcgaaa
     5581 agaattcact ccatggcgga cctatagatc taagtctgtc aagtatgcta agctcaaaga
     5641 cgatacagat cagattgtag cagtggctcc gattaaacta gatgatgttg tcttggttag
     5701 tcaaaatggt tatgccctgc gtttcaatat cgaagaggtt ccggttgtcg gtgctaaggc
     5761 agcaggtgtc aaggctatga atttgaaaga agatgatgtc ctccaatctg gctttatctg
     5821 taatacttcg tccttctacc tcttgaccca gcgtggaagc ttgaaacgtg tttctattga
     5881 ggaaattcta gcaaccagcc gtgccaaacg aggattacaa gtcttgcgtg agttgaaaaa
     5941 caaaccgcat cgtgtcttct tggcaggagc agttgcagag caaggatttg ttggcgattt
     6001 cttcagtacg gaagtggatg tgaacgacca aactctgctt gtccaatcca ataaaggaac
     6061 aatctatgaa agccgattgc aagacttgaa cttgtcagaa cgcactagca atggaagctt
     6121 catttctgac acgatttcag atgaagaagt ttttgacgct tatcttcagg aagtagttac
     6181 tgaagataaa taagtgaaat gaagaatcag ttctaagagc tggtttttat tggagaaatt
     6241 ttctgaaaat tacaaaatat acttgctatt ttagaaaaat agtgtagaat ataagaaata
     6301 ggtttttaga ataaggagtg gaatatgaca gtaacgattg attgggaaaa cctcggtttt
     6361 tcctatatga aattacctta tcgctatctt gctcatttca aaaatggaca atgggatcaa
     6421 ggagagctta cagaggatgc aactttgcat atttcagagt cttctccaag tcttcactat
     6481 ggacaacaag catttgaagg tttgaaagct tatcgtacta aggatggcag tgttcaactg
     6541 ttccgtcctg atgaaaatgc taaacgtctg caacgtacat gtgaccgtct cttgatgcca
     6601 caagttccga cagacatgtt tgtagaagct tgtaaagcag ttgtccgtgc gaatgaagaa
     6661 tacgtaccac catacggaac aggtggaacc ttatatcttc gccctctttt gattggtgtc
     6721 ggagatatta tcggggtaaa accggcagaa gagtacattt tcaccatctt tgctatgcca
     6781 gttggaaatt actttaaagg tggtttggtc ccaaccaact tcttgattca ggatgagtac
     6841 gaccgtgcag caccaaatgg tacaggtgcg gctaaggttg gtggaaacta tgctgcaagt
     6901 ctcttaccag gaaaaatggc caagtcacgt catttctcag atgttatcta tctggaccca
     6961 tcaactcata caaagattga agaagtcgga tcagctaact tctttggaat tacagctgat
     7021 aatgaatttg taacaccatt gagtccatct atcttgccat ctattaccaa gtattccttg
     7081 ctttatttgg cagaacatcg cttgggatta actcctattg agggtgatgt tccaattgat
     7141 aatcttgacc gttttgtaga ggcaggtgcc tgtggtacag cagcggttat ttctccaatt
     7201 ggaggtattc aacatggtga tgatttccat gtattctata gtgaaacaga agtaggtcct
     7261 gtgacgcgta aattatataa tgaattgacg ggtattcagt ttggcgatat tgaagcgcca
     7321 gaaggttgga ttgtaaaagt agattaaaat aaaccaaagg agatttttta tgaaatagaa
     7381 aaagtggctc ttaacagcag gagtggtcct gagcacgtca gctattttag tggcttgtgg
     7441 aaaaactgat aaagaagcag atgcaccgac aacattttct tatgtctatg cagtagatcc
     7501 agcatcattg ggctacagta tagcgactcg aacatcgagg acagacgtta ttggaaatgt
     7561 tattgatggt ttgatggaaa atgataaata cggcaatgtt gctccttctc aaaaagacta
     7621 tgatttgaac agtacaggat gggctccaag ctatcaagat ccagcgtctt acttgaatat
     7681 tatggatcca aaatctggtt ctgacatgaa acaccttggc attacgaaag gaaaagataa
     7741 ggatgttgta gctaaacctg gtttggataa atataagaaa ttgttagaag atgctgtttc
     7801 tgagaccact gacctagaga agagatatga aaaatatgcc aaagctcaag cttggtcgac
     7861 agatagttca ttattgatgc caacagcttc atctggtggt tttccagttg taagtaacgt
     7921 agtaccattc tcaaaaccat actcacaagt tggtattaag ggggaaccat atatctttaa
     7981 aggaatgaaa ttgcaaaaag atattgttac aacaaaagaa tataacgagg tttttaaaaa
     8041 atggcaaaaa gaaaaattgg aatccaatag caaataccaa aaagaactag aaaaatacat
     8101 taaataagga atggtattga tcttgataaa atttttaaaa tactgtcatt ttgaatataa
     8161 aggagtttga tatggagtgg attagattaa taggaatagc aatcattgtt gtgggtttta
     8221 ttttaaaatt tgatacaatt gcaacagtag tcttagctgg tttggttaca gctttagttt
     8281 caggtgtttc tctcgttgaa tttttggaga ttttgggaaa agaatttagc aatcagcgag
     8341 tgctcacgat ttttatggtt accttgcctc ttgtggggct gtcagaaacc tttggactca
     8401 agcaacgatc aatcgatttg attcgaaaga ttaaaggtct gacagttgga aacttctata
     8461 cagtttattt ctttattcga gagttagctg gtttcttttc aattcgtcta ggaggacacc
     8521 ctcagtttgt cagacctttg gttcaaccta tgggagaagc agctgcagag tctcaattag
     8581 gtagaaagtt aacagaggtt gaagatgaga caataaaagc gcgtgcggct gcgaatgaaa
     8641 attttggaaa tttctttgct caaaatacgt ttgtaggtgc tgggggagtc ctcttgatag
     8701 ggggaacatt agatcagtta ggctatgaaa gcaattatgc agggattgct tctacatcta
     8761 ttattgttgc tgttataaca cttattgtag tggggattta caattattta tttgataaaa
     8821 aattgatatc aaaaaagact aggggaggag aacaaaaatg acagagttgg caaagcaact
     8881 attagagttg acctatattg tgattggttg tcaatttctc catacagcct attgtagtta
     8941 taaagataaa acaaacccag ttcgacttgg gacatctgca ttttggactc tattgtctat
     9001 tacgtttata ggtggttcct atatgccaaa tatgagtatt ggtattattg taatcctatt
     9061 atcgctgtta acattgttta agcaagtccg tatcggaacc ttgccatcct tagatgaaat
     9121 gaaagccaat attgaatcta acaggttgaa aaataaaatt tttattccag ttatgctgat
     9181 ggcaatactt gcgttggtct tagcgcaaat gattccagaa tttagcaaga tttcgattag
     9241 ccttgccgcc ttgtttgcta caatttctgt tcttgtgatt accaatagtc accctaagag
     9301 tctgttatca gaaaataatc gaatgactca gcaagtttca acaagtggga ttgttcctca
     9361 attattaggg gctttggggg ctatttttac tgtagcaggt gttggtgatg ttatctctca
     9421 tctgattagc ggtattgttc cttcagatag tcgctttata ggagttttgg cttatgttct
     9481 tggaatggtt ctattcacaa tgattatggg aaatgctttt gcagcattca ccgttattac
     9541 agcaggtgtt ggagttccct ttgtatttgc tctgggagct aatccaattg tggctggtgc
     9601 tcttgccatg acagcaggtt attgtgggac cttattgacc ccaatggctg ctaattttaa
     9661 cgctctacca gcagcattga tggatatgaa agatcagaat ggcgttataa aggctcaagc
     9721 aggtgttgct ctagtaatga ttgttattca catattctta atgtactttc tcgcatttta
     9781 gtaaaggaaa ataaaatgaa aatattggtt acaggtttta atccttttgg aggtgaaaag
     9841 attaatccag ctttggaggc tgtaaaatta ttaccatctg agattaatgg ggctgaagtt
     9901 cgctgggtag aaattccaac ggttttttat aagtcgtcag aagttttaga ggcagaaata
     9961 ttacgatatc aaccagatgc tgtactttgt attggacaag caggcggcag gaccggctta
    10021 acacctgaac gagtggctat taatcaagat gatgctcgca tacctgataa cgaaggcaat
    10081 caaccaattg atacaccgat tcgtattgat ggagcatcgg cctattttag tagtttacct
    10141 atcaaagcga tggtacaagc tatcaaaaaa cagggacttc cggcagttgt atccaatagt
    10201 gcaggaacct ttgtttgcaa tcatttgatg taccaagctc tttatttagt ggataaaaaa
    10261 ttccctaata tgagagcagg tttcatgcat attccatata tgatggaaca agtagtaaat
    10321 aagccgaata ctgcaggaat gagtctatgt gatattgtaa gaggcataga ggtagctatt
    10381 gaagctattg tagactacaa agataaggat ttgcagttag taggcggtga gactcactga
    10441 taacctaaaa aggatagtca attatgcttg tttactaact attaactatg ctaaatcaat
    10501 tgaggttgtt tacataaaac tctatatcag agaagcctga tatagagttt tttcttgcta
    10561 gttttaggat tttcttgtaa aatagaaaaa gtgaagagag gtatgaaatg agcaagaaag
    10621 ataaaaaaat cgaaattcaa gtagcggatg ccaaagttaa tgttggtaaa gacagttttg
    10681 aaggttatac attgactatc ggtaaaaaag ttatcggaga aattgccgaa ttagacggac
    10741 aatttgccat tataaagaat gggaatgtcg atagttttta taaaaaattg gaaaaagctg
    10801 tggaaatttt gattgaaaat tataatttag caaaataagt cttgtttttt tgaaattttc
    10861 atgatataat agtccatgtt gattgtagga gagatagcga agaggctaaa cgcggcggac
    10921 tgtaaatccg ctccttcggg ttcgggggtt cgaatccctc tctctccatt tcattaatgg
    10981 ggtatagcca agcggtaagg caagggactt tgactccctc atgcgttggt tcgaatccag
    11041 ctaccccagt tcttaggtaa taatcaagat aaaaagcaaa atatcttagg gtattttatt
    11101 tttataattg aaagacgtga atgatatgaa catgtccttg cgggtgctta ggaaaaaaat
    11161 tataagtatg tcaagtttaa gaaaaacttg attgttggag gattttttag atgaacgaat
    11221 ttgaagattt gctaaatagc gttagtcaag ttgagactgg tgatgttgtt agtgctgaag
    11281 tattgacagt tgatgcgact caagctaacg ttgcaatctc tggaactggt gttgaaggtg
    11341 tcttgactct tcgcgaattg acaaacgatc gtgatgcaga tatcaatgac tttgttaaag
    11401 taggagaagt attggatgtt cttgtacttc gtcaagtagt tggtaaagat actgatacag
    11461 ttacatacct tgtatctaaa aaacgccttg aagctcgcaa agcatgggac aaacttgttg
    11521 gtcgcgaaga agaagttgtt actgttaaag gaacgcgtgc cgttaaaggt ggactttcag
    11581 tagaatttga aggtgttcgt ggatttatcc cagcttcaat gttggatact cgtttcgtac
    11641 gtaacgctga gcgttttgta ggtcaagaat ttgatactaa aatcaaagaa gttaacgcta
    11701 aagaaaaccg cttcatcctt tcacgtcgtg aagttgttga agcagctact gcagcagctc
    11761 gcgctgaagt attcggtaaa ttggctgttg gtgatgttgt aactggtaaa gttgctcgta
    11821 tcacaagctt cggcgctttc atcgaccttg gtggtgttga cggattggtt cacttgactg
    11881 aattgtcaca tgaacgtaac gtatcaccaa aatcagttgt aactgttggt gaagaaattg
    11941 aagtgaaaat ccttgatctt aacgaagaag aaggacgtgt atcactttca cttaaagcaa
    12001 cagtaccagg accatgggat ggcgttgagc aaaaattggc taaaggtgat gtagtagaag
    12061 gaacagttaa acgtttgact gacttcggtg catttgttga agtattgcca ggtatcgatg
    12121 gacttgttca cgtatcacaa atttcacaca aacggattga aaatccaaaa gaagctctta
    12181 aagttggtca agaagttcaa gttaaagttc ttgaagttaa cgcagatgca gaacgcgtgt
    12241 cactttctat taaagctctt gaagaacgtc cagcccaaga agaaggacaa aaagaagaaa
    12301 aacgtgctgc tcgtccacgt cgtccaagac gtcaagaaaa gcgtgatttc gaacttccag
    12361 aaacacaaac aggattttca atggctgatt tgtttggtga tatcgaactt taatcaaatt
    12421 gaaaattcac aaaatccttt gtttactaaa caagggattt ttcttttgtc tctgctcctt
    12481 ttttgatata atagttctat gttaaaatca gaaaaacaat cacgttatca aatgttaaat
    12541 gaagaattgt ccttcctatt ggaaggcgaa accaatgttt tggctaatct ttccaacgcc
    12601 agtgctctta taaaatcacg ttttcctaat accgtatttg caggctttta tttgttcgat
    12661 ggaaaggaat tggttttagg tcccttccaa ggaggtgttt cctgcatccg tattgcacta
    12721 ggcaagggtg tttgtggtga ggcagctcac tttcaggaaa ctgttattgt tggagatgtg
    12781 acgacctatc tcaactatat ttcttgtgat agtctagcta aaagtgaaat tgtggtgccg
    12841 atgatgaaga atggtcagtt acttggagtt ctggatctgg attcttcaga gattgaggat
    12901 tacgatgcta tggatcgaga ttatttggaa caatttgtcg ctattttgct tgaaaagaca
    12961 acatgggact ttacgatgtt tgaggaaaaa tcttaatgta tcaagcactt tatcgaaaat
    13021 atagaagtca aaacttctcc cagttagttg gtcaagaagt tgtggctaag actcttaaac
    13081 aagcggtgga gcaagagaaa ataagtcacg cttatctttt ttctggtcct cgtggaacgg
    13141 gaaaaaccag tgttgctaaa atctttgcca aggctatgaa ctgtcccaat caagtgggtg
    13201 gcgaaccttg caataactgc tatatttgtc aagcagtgac ggacggtagt ttagaagatg
    13261 tcattgaaat ggatgcagct tctaataatg gggtagatga aattcgcgaa attcgtgata
    13321 aatctaccta tgcgcctagc cttgctcgtt ataaggttta tatcatagat gaggttcaca
    13381 tgctgtctac aggggctttt aatgccctcc taaagacgct ggaagaacca acacagaatg
    13441 tagtctttat tttggccact actgaattac acaagattcc tgctactatt ctatcccgtg
    13501 tgcaacgttt tgagtttaaa tcaattaaga cacaggatat taaggaacat attcactata
    13561 tcttagaaaa agaaaatatc agttctgaac cagaggctgt ggaaatcatt gccagacggg
    13621 cggaaggtgg aatgcgggac gccttgtcta ttttggatca agccctgagt ttgacacagg
    13681 gaaatgagct gacgactgct atctctgaag aaattactgg cactattagc ctatcagcct
    13741 tggatgatta tgtggcggcc ttgtctcaac aggatgttcc caaagctttg tcttgcttga
    13801 atcttctttt tgacaatggt aagagcatga ctcgttttgt gaccgatctt ttgcactatt
    13861 taagagactt gttaattgtt caaacagggg gagaaaatac tcatcatagt tcagtctttg
    13921 tagaaaattt ggcacttcct caaaaaaatc tgtttgaaat gattcgctta gcaacagtga
    13981 gtttagcaga tattaagtct agtttgcagc ccaagattta tgctgaaatg atgaccgtcc
    14041 gtttggcgga aatcaagccc gaaccagctc tatcaggagc ggttgaaaat gaaattgcta
    14101 cgctgagaca ggaagttgcc cgtctcaaac aagagctttc taatgtaggt gcggttccta
    14161 aacaagttgc accagctcct agtcgaccag ctacgggcaa aacagtctat cgtgtcgatc
    14221 gcaataaagt gcaatctatc ttacaagagg ccgtcgaaaa tcctgattta gcacgtcaaa
    14281 atctaattcg tttgcagaat gcctggggag aggtaattga aagtctaggt gggccggaca
    14341 aggctctgct agttggttct caaccggttg ctgccaatga acaccatgct attcttgctt
    14401 ttgagtctaa cttcaatgct ggtcaaacta tgaaacgaga caatctcaat accatgtttg
    14461 gcaatatcct cagtcaggcg gcaggttttt cacctgagat tttagctatt tccatggagg
    14521 aatggaaaga agttcgcgca gccttttcag ccaaagccaa atcttctcaa actgaaaaag
    14581 aagtagaaga aagcctgatt ccagaaggat ttgaattttt ggctgataaa gtgaaggtag
    14641 aggaagacta aagaaagatt tcgtgataca ataagtttat gaataaacaa caatttatta
    14701 ttatggcgct gtttacagct gctgagacct attttttcaa tgaagcctgg atgactggcc
    14761 gctatattat ggcagccttt tgggcaattt tactctttag aaatttccga gtcagttatg
    14821 tgatgggcaa aatcgttgat gtcatcgatc agcattttaa taggaaagac tagccctcag
    14881 cttccagaca aaatcaaagc cttttaggct tttttttgtt atactagaaa agtatattta
    14941 tagaattttt gctctatttc tggggaaatc agacgttttt ctagtaagta ctgtaaaagt
    15001 tttgaaaaag aaaggaacta tcatgtcagt attagagatc aaagatcttc acgttgagat
    15061 tgaaggaaaa gaaattttaa aaggggttaa cctgaccctg aaaacaggag aaattgccgc
    15121 tatcatggga ccaaatggta caggtaaatc gactctttct gccgctatca tgggaaatcc
    15181 aaactatgaa gtaactaaag gtgaagtttt gtttgatggc gtaaacatcc ttgagttgga
    15241 agtggatgag cgtgcgcgta tgggactttt ccttgctatg caatacccat cagaaatccc
    15301 tggaattacc aatgctgagt ttcttcgtgc cgctatgaat gcgggtaaag aagatgatga
    15361 gaagatttca gttcgtgagt ttattactag gctagatgaa aaaatggaat tgctcaacat
    15421 gaaagaagaa atggcagagc gttacctcaa cgaaggcttc tctggtggtg agaaaaaacg
    15481 caatgaaatt cttcaacttt tgatgttgga gccaacattt gctcttttgg acgagattga
    15541 ctcaggtctt gatattgacg ctcttaaagt tgtgtctaaa ggtgtcaatg ccatgcgtgg
    15601 tgaaggtttt ggtgctatga tcatcactca ctaccaacgt cttttgaact atatcacacc
    15661 tgatgtggta cacgtgatga tggaaggtcg tgttgtcctt tctggtggtc cagaattggc
    15721 tgcgcgtttg gaacgtgaag gatacgcaaa attagctgaa gaacttggct acgactacaa
    15781 ggaagaattg taatttcctc gtatctttta ggagaagtaa atgactagag aaaatattaa
    15841 acttttttca gaaatgcacg ctgaaccaag ctggttggct gatctccgtc aaaaagcttt
    15901 tgacaagatt gagactttgg aattaccagt tattgagcgt gtcaaattcc accgttggaa
    15961 tctgggtgat ggaacgatta cagaaaatga gccatcagca aatgttccag atttcacagc
    16021 tttagatcat cacttgaagt tggtgcaagt aggaactcaa actgttttcg aacaaactcc
    16081 agttgagtta gctgaacagg gtgttgtctt cacagacttt cactcagctt tagaagaaat
    16141 tccagagctg atcgaagaat tcttcatgtc atctgttaag tatgatgatg acaagttggc
    16201 ggcttaccac acagcttact ttaacagtgg tgctgtactc tatattccag ataacgtaga
    16261 aatcacagag ccaattgaag gaattttcta ccaagatagc gatagcaatg tgccgtttaa
    16321 caagcatatt atgattatcg ctggtaaaaa ttctaagatt aattatctgg agcgtttaga
    16381 gtcacgcggt gaaggaagtg ccaaagcaac tgctaatatc acagtagaag tgattgcacg
    16441 ttcaggtgcg caagtcaagt ttgctgctat cgaccgtcta ggtgaaaacg tcactgccta
    16501 cattagccgt cgtggtaaat taggcaacga tgcaagtatt gactgggcta tcggtgtcat
    16561 gaacgaagga aatgtcgttg ctgattttga tagtgacttg attggtaatg gtagctatgc
    16621 tgacctcaag gttgtagctc tttcaagtgg tcgtcaggta caagggattg atactcgtgt
    16681 aactaactat ggctgcaact ctatcggaaa tatcctacaa catggggtta tccttgaaaa
    16741 agcaactttg actttcaatg gtatcggcca catcatcaag ggtgctaagg gagcagatgc
    16801 ccaacaagag agccgtgttc tcatgctttc agaccaagcg cgttcagatg ctaacccaat
    16861 tcttttgatt gatgaaaatg atgtcactgc aggacacgca gcgtctatcg gacaagttga
    16921 tccagaagac atgtattacc tcatgagtcg tggattggat aaggcaactg cagagcgttt
    16981 ggttgttcgt ggtttccttg gatctgttat cgtggagatt ccagtcaagg aagttcgtga
    17041 tgaaatgatt gcaactatcg aagagaaatt gtcaaaacgc taaggggaag cctatgttag
    17101 atgtagaagc gattcgcaag gattttccaa ttttagatca gattgtcaat gatgaacctc
    17161 tggtctatct ggacaatgct gcgacgacac aaaaaccact agtagttctg aaagctatta
    17221 acagttacta tgagcaggac aatgccaatg ttcaccgtgg tgtccatacc ttagcggaac
    17281 gagcgacagc ttcttatgaa gctgctcgtg aaaccattcg taagtttatt aatgcaggct
    17341 ctacaaagga agttctcttt accagaggaa cgacaaccag ccttaactgg gtggcacgct
    17401 ttgctgagga aattctcact gagggagacc aggtcttgat ttcagtaatg gaacaccatt
    17461 ctaatatcat tccatggcag gaagcttgtc gaaagactgg agcagagctt gtctatgtct
    17521 atcttaaaga cggtgccttg gatatggagg atttgcgagc taaattgact gataaggtta
    17581 aatttgtttc cctagctcat gcctccaatg ttcttggtgt ggtcaatccg atcaaggaaa
    17641 tcactcaatt agcccaccaa gttggggcaa tcatggtagt ggatggtgct caatctacac
    17701 ctcatatgaa gattgatgtc caggacttgg atctggactt tttcgccttt tcgggtcaca
    17761 agatggctgg tccgactggt atcggtgtcc tttacggcaa agaaaagtat cttgagcaaa
    17821 tgtctccagt agaatttggc ggcgagatga ttgattttgt ctacgagcaa tccgctagtt
    17881 ggaaggaatt gccttggaaa tttgaggctg gaacgccaaa tatggcagga gctattggac
    17941 ttgcgactgc agttgattat ctggaaaaga ttggtatgga tgccgttgaa gctcatgaac
    18001 aggaattgat tgcgtacgtc tatccaaaac tgcaggcaat tgagggattg accatttacg
    18061 gttctcagga tttggctcaa cgttcgggtg ttattgcctt taacctaggt gatctccatc
    18121 ctcacgatct tgcgacggct ctggattatg aaggagtggc tgttcgtgct ggtcaccatt
    18181 gtgcgcaacc cttgcttcag tatttggaag tcccagcatc agctcgtgca agtttttata
    18241 tctataatac caaggcagat tgcgacaaac tagtcgatgc cctacaaaag acaaaggagt
    18301 ttttcaatgg cactttctaa actagatagc ctttatatgg cagtggtagc agaccattcg
    18361 aaaaatccac atcaccaagg gaagttagaa gacgctgagc aaatcagtct caacaatccg
    18421 acttgtgggg atgtcatcaa cctctctgtc aagtttgatg cagaggaccg tttggaagat
    18481 attgcttttc taaattcagg atgcacgatt tcaactgctt ctgctagtat gatgacagat
    18541 gccgttttag gaaaaaccaa acaagaaatt ttagaactgg cgactatttt ttctgaaatg
    18601 gttcaagggc aaaaagatga gcgtcaagac caacttggag acgcggcatt cttgtcaggt
    18661 gttgccaaat tccctcaaag aatcaagtgt gcaaccctag cttggaatgc ccttaagaaa
    18721 acaattgaaa atcaagaaaa acagtaagac aagtttcttt tgtcttatga attattagaa
    18781 atgaagaaag gatattatgg ctgaagaaag agtagaacca aaaccaattg accttggtga
    18841 atataaattt ggtttccatg acgatgtaga gcctgtctta tcgacaggaa aaggactcaa
    18901 cgaaggtgtt attcgtgaat tatctgctgc taagggtgag cctgagtgga tgttggagtt
    18961 ccgtttgaag tcttatgaaa ccttcaaaaa aatgcccatg caaacttggg gagcagactt
    19021 gtcagagatt gactttgatg acttaatcta ctaccaaaaa ccatctgaca aaccagcccg
    19081 ttcttgggat gatgtacctg aaaagattaa agaaaccttt gaacgtatcg ggattccaga
    19141 agctgaacgt gcttatttag caggggcttc tgcccagtac gagtcagaag tggtttacca
    19201 caacatgaag gaagagttcc aaaaattagg tattatcttt acagatacag attccgcact
    19261 caaggaatac ccagacttat ttaaacaata ctttgcgaag ttggtaccgc cgacagataa
    19321 caagttggca gccctcaact cagcagtatg gtcgggtgga acttttatct acgtgccaaa
    19381 aggtgtcaag gtagatattc cacttcaaac ttatttccgt atcaataacg aaaatatagg
    19441 tcagttcgaa cgtaccttga ttatcgttga tgagggagca agcgtccact acgtagaagg
    19501 atgtacagca ccaacatatt caagcaatag cttgcacgct gccattgtag aaatttttgc
    19561 tttggacgga gcttatatgc gttatacaac tatccaaaac tggtctgata acgtctataa
    19621 cttggtaaca aagcgtgcta aggctcaaaa ggatgccact gttgagtgga ttgatggaaa
    19681 cttgggtgcc aaaacgacta tgaaatatcc atctgtttat cttgatggag aaggagcgcg
    19741 tggtaccatg ctctctatcg cctttgctaa tgcagggcaa caccaagaca cgggtgctaa
    19801 gatgattcac aatgctccac ataccagctc gtctattgtg tctaaatcca tcgctaaagg
    19861 tggaggaaag gttgactacc gtggacaagt cacctttaac aagaactcta agaaatctgt
    19921 ttcccacatt gaatgtgata ccattatcat ggatgacttg tcagcatcag atactattcc
    19981 atttaatgaa attcacaact cgcaagtggc tttggaacac gaagccaaag tatctaagat
    20041 ttcagaagag caattgtatt atctcatgag ccgtggattg tcagaatctg aggcaactga
    20101 aatgattgtc atgggatttg tagaaccctt tacaaaagaa cttccaatgg aatacgcagt
    20161 tgagctgaac cgcttgatta gctatgaaat ggagggatca gttggataaa atttgatttt
    20221 atactcttcg aaaatctctt caaaccacgt cagcatcgcc ttaccgtatg tatggttact
    20281 gacttcgtca gtttcatcta caacctcaaa acagtgtttt gagcaacctg cggctagctt
    20341 cctagtttat tctttgattt tgagtattag atttactcaa aatcaaggat tttgaagatg
    20401 aacttgtatc aaaaaatcgc ggtttaaaat cgcgattttt tataatttct cgttaacaaa
    20461 gcggacaaac tgattccacc aaacttttaa gaagaaggct ttttcaattt tcttgtctgc
    20521 taccatttcg aaactagggc gctctgtggt gatgtaacct tgaccaatca agtccttgtc
    20581 ttcataagtc aaatggccaa ccactgttcc agcttcaagt ggtgctggga ttgctttgga
    20641 atcaggtgtg aattgaacag attgggaaga ttgattccca acacgttcga ttagatagat
    20701 atcctctgga gccactgcag ttactgtatc ttcttttcca tcttgtacag gggctttgct
    20761 atcttgatag gcatcgcctt gttgaacgat tttgcgaagt gtaaatgtag aagaaatata
    20821 atccattagg gaagatgtag ctgtaaatcg agcgtaagga ttattgtctt gatgatctgc
    20881 atttaaaaca actgtgatga ctctcatgcc tttttcgaca gtagtaccaa caaaagactc
    20941 tccagcctta tctgttgttc ctgtttttag cccatcaaaa ccaccacggt aagcaggcat
    21001 accttctaac atgtagttgg ttgaagtgat tgtcatccca gcaaaagtag aagaaggttt
    21061 tttggtgatt tctaagactt gtgggtattt tttgatgagg ttgcgagcaa cgatagcgac
    21121 atcataagca ctaagcttat tttcctcatc ttttttagaa cctgggtaaa tgttatcccc
    21181 tagagtttca ttgttaagac ctgtcgtatt gacaacagtg gcatcctgaa ttccccattc
    21241 caagagtttt gcccgcatca tatcgatgaa atctttttct gagccagcaa ttttctcagc
    21301 tagggcaata gcggcgctgt tggcactaga taccagagtt gcttcaagca actcttcgac
    21361 agtataatta cgggcctcca taggaatatt actggcttca gaatttgtcg tcaattgata
    21421 aggataatca gaaatatcta caggagtgga gagggtaata cttccgtttt ccaaagcttc
    21481 atagaccaga taagcagtaa tcaattttgt tatggaagca atttcgacag gttgcgttgc
    21541 atccttctca tagagaattt taccagtatt tgcctcaaca gcaatcgcat gtttagcggc
    21601 aatggtaaaa tcttgagcaa cagcagtaga agcaccccct aaaagagaga cagttaacaa
    21661 agttaaaaat atttttttca tagtagtctt attctatcat aaagaaaaaa aatattcttg
    21721 ctttaataat tcatctgtta agctttttga aaatatggta aaataaagtg agggaggtaa
    21781 ctcatgtttc gtagaaataa attatttttt tggaccacag aaattttact cttaaccatc
    21841 atcttttacc tatggagaca gatggggtct ttgattaacc cttttgttag cgtgcttaat
    21901 acaattatga ttccattttt attagggggc tttctttatt atttgacaaa ccctattgtt
    21961 actttcttaa ataaagtctg taaactcaat cgtttgcttg gtattttaat taccttgtgt
    22021 actttggtct ggggaatggt cataggtgtt gtctatctct tacctatttt gattaatcag
    22081 ttatctagtt tgattatatc tagtcaaact atttatagtc gagtacaaga cttaatcata
    22141 gacttatcta attatcctgc gctccagaat ttggatgtag aagctacaat tcagcagtta
    22201 aacttatcct atgttgatat tcttcaaaat atcctaaata gcgtatcaaa tagtgtgggg
    22261 agcgtcttgt cagctcttat cagtactgtt ttgattttga ttatgactcc agtttttttg
    22321 gtttatttct tattagatgg acataaattc ttgcccatgc ttgaaagaac gattctaaag
    22381 agggatcgct tgcatattgc aggcttatta aagaatttaa atgcgacgat tgctcgctat
    22441 attagtggag tttcgattga cgcaatcatt ataggttgtt tggcttatat tggctatagt
    22501 attattggtt taaaatatgc tttagttttt gccatttttt ctggtgtagc caatttaatt
    22561 ccttatgtgg ggccaagtat tggtttgatt cctatgatca tcgcaaatat attcactgat
    22621 ccccatagac tgctgattgc agtgatttat atgcttgttg ttcagcaggt agatggcaat
    22681 atcttatatc ctcgaatcgt aggaagtgtt atgaaggttc atccaatcac gattttagtt
    22741 ttacttttgt tgtcaagcaa tatctatggt gtagttggaa tgattgtcgc agtgccaacc
    22801 tattctatct tgaaagaaat ttctaagttc ttatcccgtt tgtatgaaaa tcataaaata
    22861 atgaaagaac gagaaagaga attagctaag taaaagtcag gagaaccctg atttttcttt
    22921 actggaagtg gcctttagat tagaagactg aaaataagtt aaagtcttaa actaattttc
    22981 acagctaaga atagtagaag ttaatctgat aaaaatcgaa aaaaccagtg gaattctgtg
    23041 tcagggtaag ttccactggt tttcatagtc tattaaagtt cgaatgaaac ctatttatag
    23101 tagattgaaa ctagaatagt acacctctaa ttctaaaaca ttgttagaaa tcgatttgac
    23161 tgtcctgatc tattcgttct attcttattt cattttacta tattttggtg caataagtga
    23221 aaagtagtcc gaataatata aggattgatt ttatagtttt taaactcaaa tgaattgaaa
    23281 taaagagagt acgaaaattc tcatctgaaa gtattttaga ataattctct tcgtgaattt
    23341 cttcaaaaca gatagcttca tcttaggtat gtgatttctt tttgcatttt tgagttagat
    23401 aaggtataat ggttttattg tcttttgggg tcgttacgga ttcgacaggc attatgaggc
    23461 atattttgcg actcgtgtgg cgacgtaaac gctcagttaa atataactgc aaaaaataac
    23521 acttcttacg ctctagctgc ctaaaaacca gcaggcgtga cccgatttgg attgctcgtg
    23581 ttcaatgaca ggtcttatta ttagcgagat acgattaagc cttgtctagc ggtttgataa
    23641 gagattgata gactcgcagt ttctagactt gagttatgtg tcgaggggct gttaaaataa
    23701 tacataacct atggttgtag acaaatatgt tggcaggtgt ttggacgtgg gttcgactcc
    23761 caccggctcc attattcctt tgcattcttt tgcattcctt ggtaaaacgt tgttaaatca
    23821 acgtttttta tttttatctt tggtattcct ttgcattctt ttgctaaaaa gggagtcaca
    23881 aacagaccct attttaaaaa aaggatagaa aaaaggatac aacatttgtc gcatcctaaa
    23941 aataatcttt tttccacgga agacatggga ttcgaaccca cgcacgctat tacacgccta
    24001 ccgcgtttcc aacacggcct cttaagcctc ttgagtaatc ttccaatact tactcaaata
    24061 gtctaccata aaggctctta tcttgcaata aaaattctag aaataagaaa aatgatagat
    24121 tttgaaagaa aatgataaaa aatgcttgac ttcaaaagaa agtatgatag aatgaatagt
    24181 gtaaacgata acaggaggtg attcggtgtt aaaaacagag cggaagcaac tgattttaga
    24241 ggagttaaat caacatcatg tagtttctct agaaaaatta gttagtttgc tagaaacgtc
    24301 agaatcaacg gttcgaagag acttggatga gttggaagcg gaaaacaagc ttcgtcgtgt
    24361 gcatggtgga gcagaactcc cccactcctt acaggaagaa gaaaccattc aagaaaaatc
    24421 tgtcaaaaac cttcaagaaa agaagttgct ggctcagaaa gcagcctctc tcattaaaga
    24481 aaaagatgtc atctttatcg atgctggaac aacaactgct tttttgattc atgaattggt
    24541 caataagaat attacagttg tgaccaactc cattcaccat gccgctcagt tggttgaaaa
    24601 gcagattcca actgtcatgg ttggaggaaa cgtcaagacg gcgacagatg ctagtatcgg
    24661 tggcgttgct cttaaccaga ttaaccaatt gcactttgac cgtgccttta tcggaatgaa
    24721 tggtgttgac gatggctatt atacgactcc tgatatggag gagggagctg tgaaaagagc
    24781 tattttggag aatgccaagc agacctacgt cttggtggat tcgtcaaaaa ttggacaaac
    24841 ttgctttgcc aaggtagcac cactcaaacg cgctatcgtt atcactagtc aagggcatga
    24901 gctcttgcag gttattaagg agaaaacgga ggtaatagaa gtatgattta tacagtcaca
    24961 ctcaatccat ccattgacta tatcgttcgt ttggaccaag tcaaagttgg tagtgtaaat
    25021 cgtatggaca gtgatgataa gtttgctggt gggaaaggaa tcaatgtcag ccgtgtcttg
    25081 aaacgtttga atataccaaa tacagcgacg ggatttatcg gtggctttac tggtaaattt
    25141 atcacagata ctttagcaga ggaagaaatc gagacacgtt ttgtccaggt ggcagaagat
    25201 actcgtatca atgttaaaat caaagcagac caagaaacag aaatcaacgg aacgggtcca
    25261 actgttgaat cggttcagct agaagaattg aaagctattt tatctagtct gacagcagaa
    25321 gatacagttg tctttgcagg ttcaagtgct aaaaatctag gcaatgttat ctataaggat
    25381 ttgatttcct tgacgcgcca gactggtgcg caagtggtct gtgactttga aggacagacc
    25441 ttaattgata gtttggacta ccagcctctt cttgtaaaac caaacaatca tgaacttgga
    25501 gcgatttttg gggttaaact cgaaagttta gatgaaattg agaaatacgc tcgtgagtta
    25561 ctggctaagg gtgctcaaaa tgttattatc tctatggctg gtgatggtgc ccttcttgtc
    25621 acatctgagg gagcttactt cgctaaacca atcaaaggaa cagtcaaaaa ttcagttgga
    25681 gctggtgatt ctatggttgc tggattcaca ggtgaatttg tcaaatcaaa agacgtagta
    25741 gaagccttca aatggggagt ggcttgcgga acggcaacta ccttctcaga tgacttggca
    25801 acggcggaat ttattaaaga aacatatgga aaagttgagg tagaaaaacg atgaaaattc
    25861 aagacctatt gagaaaagat gtcatgttgc tagatttgca ggcaactgaa aaaacagctg
    25921 tcatcgacga gatgattaaa aatttgacag accacggtta tgtaacagat tttgaaacat
    25981 ttaaagaagg aattttggcg cgtgaagctt tgacttctac tggtttgggt gatggaatcg
    26041 caatgcctca cagcaaaaac gctgctgtca aagaagcgac agttctattt gctaagtcaa
    26101 ataagggtgt tgactacgag agcttggatg gacaagcaac tgacctcttc ttcatgattg
    26161 cagctccaga aggtgccaat gatactcact tggcagcctt ggcagaattg tctcaatact
    26221 tgatgaaaga cggttttgca gacaaacttc gtcaagcaac atctgcagac caagttatcg
    26281 aactttttga ccaagcttca gaaaaaactg aggaacttgt tcaagcacct gctaatgact
    26341 ctggtgactt tatcgtagct gttacagctt gtacaacagg tattgcccac acttacatgg
    26401 cccaagaagc ccttcaaaaa gtagctgctg aaatgggggt tggtatcaag gtcgaaacca
    26461 acggtgctag cggtgttgga aatcaactaa ctgcagaaga tatccgtaag gctaaagcta
    26521 ttatcattgc agcagacaag gccgttgaaa tggatcgatt tgatggaaaa ccattgatca
    26581 atcgtccagt tgctgacggt atccgtaaga cagaagagct aattaacttg gctctttcag
    26641 gagatactga agtctaccgt gccgctaatg gtgccaaagc tgcaacagcc tctaacgaaa
    26701 aacaaagcct tggtggtgcc ttgtacaaac acttgatgag tggtgtatct caaatgttac
    26761 cattcgttat cggtggtggt atcatgattg cccttgcctt cttgattgac ggtgctttgg
    26821 gtgttccaaa tgaaaacctt ggcaatcttg gttcttacca tgagttagct tctatgttca
    26881 tgaaaattgg tggagctgcc tttggtttga tgcttccagt ctttgcgggt tatgttgcct
    26941 actctattgc tgaaaaaccg ggtttggtag caggtttcgt ggctggtgct attgccaaag
    27001 aaggttttgc ctttggtaaa attccttatg ccgcaggtgg tgaagcaact tcaactcttg
    27061 caggtgtctc atctggtttc ctaggtgccc ttgttggtgg atttatcgca ggtgccttgg
    27121 ttcttgccat caagaaatac gttaaagttc ctcgttcact cgaaggtgct aaatcaatcc
    27181 ttctattgcc acttcttgga acaatcttga caggatttgt tatgctagct gtgaatatcc
    27241 caatggctgc aatcaacact gctatgaatg acttcctagg cggtcttgga ggaggttcag
    27301 ctgtccttct tggtatcgtc cttggtggaa tgatggctgt tgacatgggt ggaccagtta
    27361 ataaagcagc ttatgtcttt ggtacaggta cgcttgcagc aactgtttct tcaggtggtt
    27421 ctgtagccat ggcagcagtt atggctggag gaatggtgcc accacttgca atctttgtcg
    27481 caactcttct tttcaaagat aaatttacta aggaagaacg taactctggt ttgacaaaca
    27541 tcatcatggg cttgtcattt atcactgagg gagcgattcc atttggtgcc gctgacccag
    27601 ctcgtgcgat tccaagcttc atccttggtt cagcagtagc aggtggactc gttggtctta
    27661 ctggtatcaa actcatggcg ccacacggag gaatcttcgt tatcgccctt acttcaaatg
    27721 ctctccttta cctcgtttct gtcttggtag gagcaatcgt aagtggtgtg gtttatggtt
    27781 acctacgcaa accacaagca taaaaaatag aaaaatgaaa agattggacc gtttggtgca
    27841 gtctttttct cttcccgaaa tgcctgtgaa atatggtata atagaagaat ggcaaacaag
    27901 aatacaagta caacaagacg gagaccgtct aaagcagaac tggaaagaaa agaagcgatt
    27961 caacgaatgt tgatttcgtt aggaattgcg attttattga ttttcgcagc cttcaaatta
    28021 ggggctgcag gtataaccct ttataattta attcgcttgc tagtgggtag cctagcttat
    28081 ctggcgatat tcggcctatt aatctatctc ttctttttca agtggatacg aaaacaggaa
    28141 ggactcttat ctggcttttt caccatattt gctggcttac tcttgatttt tgaggcctac
    28201 ttggtttgga aatatggttt ggacaagtcc gttctaaaag ggaccatggc tcaggttgtg
    28261 acagatctga ctggttttcg aacgactagc tttgctggag ggggcttgat cggggtcgct
    28321 ctttatattc caacagcctt tctcttttca aatatcggaa cttactttat tggttctatc
    28381 ttgattttag tgggttctct cctagtcagc ccttggtctg tttacgatat tgctgaattt
    28441 ttcagtagag gctttgccaa atggtgggaa gggcacgagc gtcgaaaaga ggaacgcttt
    28501 gtcaaacaag aagaaaaagc tcgccaaaag gctgagaaag aggctagatt agaacaagaa
    28561 gagactgaaa aagccttact cgatttgcct cctgttgata tggaaacggg tgaaattctg
    28621 acagaggaag ctgttcaaaa tcttccacct attccagaag aaaagtgggt ggaaccagaa
    28681 atcatcctgc ctcaagctga acttaaattc cctgaacagg aagatgactc agatgacgaa
    28741 gatgttcagg tcgatttttc agccaaagaa gcccttgaat acaaacttcc aagcttacaa
    28801 ctctttgcac cagataaacc aaaagatcag tctaaagaga agaaaattgt cagagaaaat
    28861 atcaaaatct tagaagcaac ctttgctagc tttggtatta aggtaacagt tgaacgggcc
    28921 gaaattgggc catcagtgac caagtatgaa gtcaagccgg ctgttggtgt aagggtcaac
    28981 cgcatttcca atctatcaga tgacctcgct ctagccttgg ctgccaaaga tgtccggatt
    29041 gaagcaccaa tccctgggaa atccctaatc ggaattgaag tgcccaactc cgatattgcc
    29101 actgtatctt tccgagaact atgggaacaa tcgcaaacga aagcagaaaa tttcttggaa
    29161 attcctttag ggaaggctgt taatggaacc gcaagagctt ttgacctttc taaaatgccc
    29221 cacttgctag ttgcaggttc aacgggttca gggaagtcag tagcagttaa cggcattatt
    29281 gctagcattc tcatgaaggc gagaccagat caagttaaat ttatgatggt cgatcccaag
    29341 atggttgagt tatctgttta caatgatatt ccccacctct tgattccagt cgtgaccaat
    29401 ccacgcaaag ccagcaaggc tctgcaaaag gttgtggatg aaatggaaaa ccgttatgaa
    29461 ctctttgcca aggtgggagt tcggaatatt gcaggtttta atgccaaggt agaagagttc
    29521 aattcccagt ctgagtacaa gcaaattccg ctaccattca ttgtcgtgat tgtggatgag
    29581 ttggctgacc tcatgatggt ggccagcaag gaagtggaag atgctatcat ccgtcttggg
    29641 cagaaggcgc gtgctgcagg tatccacatg attcttgcaa ctcagcgtcc atctgttgat
    29701 gtcatctctg gtttgattaa ggccaatgtt ccatctcgtg tagcatttgc ggtttcatca
    29761 ggaacagact cccgtacgat tttggatgaa aatggagcag aaaaacttct tggtcgagga
    29821 gacatgctct ttaaaccgat tgatgaaaat catccagttc gtctccaagg ctcctttatc
    29881 tcggatgacg atgttgagcg cattgtgaac ttcatcaaga ctcaggcaga tgcagactac
    29941 gatgagagtt ttgatccagg tgaggtttct gaaaatgaag gagaattttc ggatggagat
    30001 gctggtggtg atccgctttt tgaagaagct aagtctttgg ttatcgaaac acagaaagcc
    30061 agtgcgtcta tgattcagcg tcgtttatca gttggattta accgtgcgac ccgtctcatg
    30121 gaagaactgg agatagcagg tgtcatcggt ccagctgaag gtaccaaacc tcgaaaagtg
    30181 ttacaacaat aaaaaaatag cttctttcca agtttggagg gaagctattt tagtggctat
    30241 tgattgcttt tattttctga agttggcgca ttggactgtt tttcgttttc agtagcaggt
    30301 ttacttgaag caggagtaga agagtcctga gttgctgttt tctgatcttc ttttttctct
    30361 tccttgacgc tagattttgg tgtttcctct tgctgtgttt tttcttgact agtgttagtc
    30421 tctttagttg gactggtgtt ttccttaggg gattcctttt ggatttcttt gacaatggtt
    30481 gtcgtctggc ttgtcgtagg ttctttttta atatttttgt tattatccaa ggcgttcata
    30541 atcgtgatac ttgcggtaat caagccaagg atactagcga ttgtagcaac ggttttttga
    30601 aagactgtaa agcctttttt gatgtgttct gtttttggtt ttgaagttct acgatagtta
    30661 gacatacatt ctctcctttg attatgattt attatactct gtttctttaa aaaaatctta
    30721 aaaaaagaga aatttccctt tcttgtcgca agctccgttt catgatacaa tagaggaagt
    30781 gattttagaa agcgtgagaa acatgattta ctttgataat tcagcgacga ccaagcctta
    30841 tcctgaagcc cttgaaacct atatgcaggt cgcttcaaaa attttaggaa acccatctag
    30901 tctccatcgt ttgggagacc aagcaacacg aatcttagat gcttctcgcc aacagattgc
    30961 agatttaatc ggtaagaaaa gcgatgaaat cttctttacc tcgggtggaa cagaagggga
    31021 taactggctt atcaagggtg tggcctttga aaaagctcag tttggcaagc acatcattgt
    31081 ttcagccatt gaacatccag cagtcaaaga gtcagccctc tggttgaaaa gtcaaggatt
    31141 tgaagtggat tttgctccag ttgataagaa aggcttggtc gatgttgagg cgttagcagg
    31201 tttgatacgg catgatacaa tcctcgtttc catcatggct gtgaacaatg aaatcggctc
    31261 tatccaacct attgaggcta tttcagaatt cttggcagac aagccgacta tttccttcca
    31321 cgttgatgcg gttcaggcgc ttgccaaaat tccgactgaa aagtatctga cagaacgggt
    31381 ggattgcgcg actttctcta gtcacaagtt ccacggggtt cgaggtgttg gctttgtcta
    31441 tatcaaatct ggcaagaaga ttacacctct tcttacaggt ggtggccagg agcgagatta
    31501 tcgttcgaca actgaaaatg tggcagggat tgcagcgaca gccaaggccc tccgtttgtc
    31561 tatggaaaag ctagatatct ttaggagcaa gactgggcag atgaaggcag tgattcgcca
    31621 agctcttctg aactatccgg atatttttgt cttttcagat gaggaaaact ttgcacctca
    31681 tattctgact tttggaatca aaggtgttcg aggtgaagtc atcgttcacg cctttgaaga
    31741 ctatgatatt ttcatctcaa caacctcagc ttgttcatct aaggcaggaa aaccagccgg
    31801 taccttgatt gccatgggag tggacaaaga taaggccaag tcagctgtgc gtcttagcct
    31861 agacttggaa aatgatatga gtcaggtcga gcagtttttg accaagttaa aattgattta
    31921 caatcaaact agaaaagtaa gataggagca ttcatgcagt attcagaaat tatgattcgc
    31981 tacggagagt tgtcaaccaa gggtaaaaac cgtatgcgtt tcatcaataa acttcgtaat
    32041 aatatttcgg acgttttgtc tatctatacc caagttaagg taacagcaga tcgcgaccgt
    32101 gcccacgctt acctcaatgg agctgattac acagcagttg cagaatctct caaacaagtt
    32161 tttggaattc aaaacttttc tcctgtttat aaggttgaaa aatctgtaga agttttgaag
    32221 tcttctgtcc aagagattat gcgggacatc tacaaggaag gtatgacctt taagatttct
    32281 agcaagcgta gcgaccacaa ctttgaactt gatagtcgtg aactcaacca aacacttgga
    32341 ggggctgtat tcgaagccat tccaaatgtg caagttcaaa tgaaaagtcc tgacatcaat
    32401 cttcaggtgg agattcgtga agaagcagcc tatctttctt atgaaaccat tcgtggggct
    32461 ggtggtttgc cagttggaac ttcaggtaaa gggatgctca tgttgtcagg agggattgac
    32521 tcacctgtag caggttatct tgctcttaag cgtggggtgg atatcgaggc agttcacttt
    32581 gctagtccac catatactag tcctggtgcc ctcaagaaag cgcaggactt gacccgtaaa
    32641 ttgaccaagt ttggcggaaa tatccagttt atagaggtgc ctttcacaga gattcaagag
    32701 gaaatcaaag ccaaagcgcc agaagcttat ttgatgactc taactcgtcg ctttatgatg
    32761 cggattactg accgtattcg tgaggtacga aatggtttgg ttatcatcaa tggggaaagt
    32821 ctaggtcaag tagccagcca aacccttgaa agtatgaagg ctatcaatgc tgttaccaac
    32881 actcccatca ttcgtcctgt ggttaccatg gacaagttgg aaatcattga catcgcccag
    32941 gaaatcgata cctttgacat ttcaatccaa ccgtttgaag actgttgtac catttttgca
    33001 ccagatcgtc caaaaacaaa tcctaaaatt aagaatgcgg agcagtacga agcgcgtatg
    33061 gatgttgaag gcttggttga gcgagcagtg gctggaatca tgattactga aatcacacct
    33121 caagccgaaa aagatgaagt tgatgacttg attgacaatc tgctctaatt cagaaaatcc
    33181 aaaagaatag cgaaaatcag taaaaaaagt tagttttttc tctaaaaaca ggtaaaaaac
    33241 taactttttt tatttttatg atataatgat ataaaatttt gaatatagag agttttctga
    33301 caatgaatca atcctacttt tatctaaaaa tgaaagaaca caaactcaag gttccttata
    33361 caggtaagga gcgccgtgta cgtattcttc ttcctaaaga ttatgagaaa gatacagacc
    33421 gttcctatcc tgttgtatac tttcatgacg ggcaaaatgt ttttaatagc aaagagtctt
    33481 tcattggaca ttcatggaag attatcccag ctatcaaacg aaatccggat atcagtcgca
    33541 tgattgtcgt tgctattgac aatgatggta tggggcggat gaatgagtat gcggcttgga
    33601 agttccaaga atctcctatc ccagggcagc agtttggtgg taagggtgtg gagtatgctg
    33661 agtttgtcat ggaggtggtc aagcctttta tcgatgagac ctatcgtaca aaagcagact
    33721 gccagcatac ggctatgatt ggttcctcac taggaggcaa tattacccag tttatcggtt
    33781 tggaatacca agaccaaatt ggttgcttgg gcgttttttc atctgcaaac tggctccacc
    33841 aagaagcctt taaccgctat ttcgagtgcc agaaactatc gcctgaccag cgcatcttca
    33901 tctatgtagg aacagaagaa gcagatgata cagacaagac cttgatggat ggcaatatca
    33961 aacaagccta tatcgactcg tcgctttgct attaccatga tttgatagca gggggagtac
    34021 atctggataa tcttgtgcta aaagttcagt ctggtgccat ccatagtgaa atcccttggt
    34081 cagaaaatct accagattgt ctgagatttt ttgcagaaaa atggtaagtt aagaaaggaa
    34141 aaaacgaaat gcatattgaa catcttagcc actggagtgg tcatcttaac cgtgaaatgt
    34201 accttaaccg ttatggacat ggtgggattc cagttgtggt ctttgcttca tcaggtggta
    34261 gtcacaacga atactatgat tttggcatga ttgatgcctg tgcttccttt atcgaggaag
    34321 gccttgtcca gttctttacc ctatctagtt tggatagtga gagctggttg gctacttgga
    34381 aaaatgctca tgaccaagcg gaaatgcacc gtgcctacga acgttatgtg attgaggagg
    34441 ccattctttt atcaagcaca agacaggttg gtttgatggc atgatgacga caggttgctc
    34501 tatgggagcc tatcatgcac tcaatttctt cctccagcat ccagatgtct ttaccaaagt
    34561 gattgctctc agtggtgttt acgacgcacg tttctttgtc ggtgattact acaacgatga
    34621 tgctatttac caaaactcgc cagtagatta tatttggaac caaaacgacg gctggtttat
    34681 tgaccgttac cgtcaggcag agattgtgct gtgtacgggg cttggagcct gggaacaaga
    34741 tggtttgcca tccttttaca agctcaaaga agcctttgac aagaaacaaa ttccagcctg
    34801 gtttgctgaa tggggacatg atgtcgccca tgactgggaa tggtggcgta aacaaatgcc
    34861 ttatttcctc ggtaatctct atttataaaa ggagttacct atgaattacc ttgttatttc
    34921 tccctactat ccacaaaact ttcaacagtt taccatcgaa ctagctaata aaggcatcac
    34981 agtcttggga attggtcaag agtcttacga gcaattggat gagcccttgc gcaatagctt
    35041 gaccgagtat tttcgtgttg ataatcttga gaacatagat gaagtcaaac gtgcagttgc
    35101 ttttctcttt tataaacatg gtccaattgg ccgcatcgag tctcacaatg aatactggct
    35161 tgagctagac gcaacactca gagaacaatt caatgttttt ggtgccaaac cagaggatct
    35221 caaaaagacg aaatataagt ctgaaatgaa gaaacttttc aaaaaagcag gtgttcctgt
    35281 ggtacctgga gctgttatca agacggaagc agatgttgat caagcagtga aagaaatcgg
    35341 tcttccaatg attgccaaac ctgataatgg agtgggagca gccgcaacct ttaaacttga
    35401 gacagaagac gatatcaatc acttcaagca agaatgggac cattcaaccc tttatttctt
    35461 tgaaaaattt gtcacttcca gcgaaatctg tacctttgac gggctcgtgg acaaggatgg
    35521 aaagattgtc ttctcaacaa cctttgacta cgcctataca ccgcttgacc tcatgattta
    35581 taagatggac aattcttatt atgtgctcaa ggatatggat cctaaactgc gcaagtatgg
    35641 ggaagcaatt gtcaaagaat ttggtatgaa agaacggttt ttccatattg agttcttccg
    35701 tgagggggac gattatatta ccatcgagta caataaccgc cctgcaggtg gttttaccat
    35761 tgatgtttat aactttgctc attccttgga cctttatcgt ggctatgcag ctattgtcgc
    35821 aggagaggag ttcccggcgt cagactttga aactcagtat tgtttggcta cttctcgccg
    35881 tgcaaatgct cactatgttt attcagaaga ggatttgctt gccaaatata gccagcagtt
    35941 caaggttaaa aaagtcatgc cagctgcctt cgcggaactt caaggagatt acctgtatat
    36001 gctgaccact ccgagtcgac aagaaatgga gcagatgatt gcagatttcg gacaacgtca
    36061 agaataagaa ctatcggatt aaggaaatta actcccttaa tccttttgtt ttgtctgata
    36121 aaaaataaga gcatcccaac aaggtagcta tcataaaact tgttcgataa ctatttgaag
    36181 caggattagg tggtcagaaa ttaaatttta atatttcaat tgagtcatag tattgtgttt
    36241 gcgtatcctt aaatcagcta aaaggatcca tgacgacacc tatacgatat agttttcaag
    36301 ataccaaaca agtctattaa tattcaatga aaatcaaaga gcaaactagg aagctagccg
    36361 caggtttctc aaaacactgt tttgaggttg tggatagaac tgacagagtc agtatcatat
    36421 acctacggca aggtgaagct gacgtggttt gaagagattt tcgaagagta taaaatattc
    36481 aggtgacgca tagatatagt taattgaagc tttgtttgaa atctgataaa ataatgatat
    36541 tactaagttt taaaaactaa agaaaaggga agatatgatt acaggcgaat taaaaaataa
    36601 aatcgatcag ctgtgggaaa ttctttggac agaaggaaac gcaaatcctt taacaaatat
    36661 tgaacagttg acttatctct tatttatgaa agatttggat agtgtcgagc ttggacgtga
    36721 aagtgatgct gaatttctag ggattcctta tgagggagtt tttccaaaag ataaacctga
    36781 ataccgttgg tcaactttta aaaatatagg agatgctcag gaagtttatc gtttaatgac
    36841 tcaggagatt tttccgttta ttaaaaatct caagggggat acagatgata cagccttttc
    36901 acgatatatg cgagaagcta tttttcaaat aaataaacct gctacgcttc aaaaggcaat
    36961 ttctatctta gatgtttttc caactagggg attagatgta gattttgata atgacaaaca
    37021 aagtattact gatatcggag atatctatga atatctgtta tcaaaattgt cgaccgcagg
    37081 taaaaatgga cagttccgta cacctcgtca catcatcgat atgatggttg agttgatgca
    37141 accgactatc aaagatatca tctcagatcc cgctatgggt tctgctggct tcttagtatc
    37201 tgctagccgt tacttaaagc gtaagaaaga tgaatgggaa accaatacag ataatatcaa
    37261 tcattttcat aatcagatgt ttcatggaaa tgatacggat acgactatgt tgagacttgg
    37321 ggcgatgaac atgatgctac atggagtaga aaatccacaa atcagttacc ttgactcgct
    37381 gtctcaagat aatgaagaag ccgataaata tactttggtt ttagcaaatc ctccttttaa
    37441 gggctcactt gactacaatt caacctctaa tgaccttctt gcaaccgtaa aaaccaaaaa
    37501 aacagaatta ctctttcttt ctcttttctt gcgaacttta aaaccaggtg gacgagcagc
    37561 agttatcgta cctgatggtg tcctttttgg ttcgtctaaa gctcataaag gaattcgtca
    37621 ggaaattgta gagaatcata agcttgatgc tgtaatctca atgcctagtg gtgtgttcaa
    37681 gccttatgct ggagtttcaa ctgccattct catctttaca aaaactggta atggtggtac
    37741 tgacaaagtc tggttttacg atatgaaagc ggatggttta agtttggatg ataagcgaca
    37801 accgattagc gacaatgata ttccagatat tatcgaacgc tttcatcatc ttgaaaaaga
    37861 agcagaacgt cagagaacgg atcaatcttt ctttgttcca gttgctgaga taaaggaaaa
    37921 tgattatgat ttgtctatca ataaatataa agagattgag tatgaaaaag ttgagtatga
    37981 accaacagag gtcatcttaa agaaaatcaa tgatttagaa aaagaaattc aagctggctt
    38041 ggctgaatta gaaaaattac tcaagtagag aggtggctgt atgaaaaaag tgaagttggg
    38101 ggaagtctta tctctaaaaa aaggcaagaa agccactgta cttgctgaac aaacaactct
    38161 aagccaacgt tatattcaaa tagatgattt aagaaataat aataatttaa aattcactga
    38221 aagtttaaat atgactgaag cactcccaga tgatattctg atagcatggg atggagctaa
    38281 tgcaggaaca gttggttatg gattatcggg agctgttggt agtacaatta cggtcttaaa
    38341 aaagaatgag cgatacaaag aaaaaattat atcagattac ttgggagtct ttttggaaag
    38401 taaatcgcag tatttacgag aacattcaac aggtgcaaca attcctcatt taaacaagaa
    38461 tatattactt gatttacaat tagaattgct aggtatcgaa gaacaagaga acattatctg
    38521 tattcttaat acgattaaaa ggcttattac taaaagaaaa ttgcagttag atgaactaaa
    38581 cttgctcgtc aaatcccgat ttaacgagat gtttggggat gttattctaa atgaaaaaga
    38641 atggaaagtt agcaaatgga acgagattct aacaataaga aatggtaaaa atcaaaaaca
    38701 ggttgaagat gctgatggga aatttcctat ttatggaagt gggggaatta tgggatatgc
    38761 taaagattgg atagtgaaga aaaattcagt tattattgga cgtaaaggaa atataaataa
    38821 acctatttta gttagagaaa atttttggaa tgtagataca gcatttggat tagagcctgt
    38881 tttagaaaaa ataaactcag aatatttatt ctatttttgt caattataca attttgagaa
    38941 gttaaataag gctgtaacaa tacctagttt aactaaatca gatttattaa atatttccat
    39001 ccccctcccc ccactagccc tccaaaatga gttcgcagac tttgtagccc tggtcgacaa
    39061 atcacaattt gcttgtgaga tagctataaa agtgtggaga aatagcttga aatttagtat
    39121 aatatagcta aactatttgt ttaaagtgag aaaaaaatgg gaaattttag ctttctttta
    39181 aaaaatgacg aatatgaatc tttttcaaaa ccttgcattg aagctgagaa tatgattgct
    39241 acatcaactg tggctactgc ctttatggcg cgtcgtgctt tagagcaggc tgtccattgg
    39301 atatatagtc acgattcata tttagaagct ccctatcgtg ctactctatc ttctttagta
    39361 tgggatgatg attttaggga tatcgtagat tctgaactcc acaagcagat agttctgttg
    39421 attcggtggg gaaaccatgc tgctcatggt ggtgaaatta aggaacgaga agcgatttta
    39481 gctttgcatc atttgtatca gtttgttaat tttatcgatt attgttacag caatgagttt
    39541 gtggagcgtt attttgatga gaagtgctta ccactttcag caaacatcaa ataccgagaa
    39601 actccacaat ctatgataaa gttacaagac agtttaccag aactgcctga ttttcatgaa
    39661 cagatggctg ctcagtccgt agaagttcaa gagacttata ctgaaaaacg tgagactgca
    39721 gcgcaacggc aagatgtgcc tttccatatt gatcaattat ctgaggcaga gacaagaaag
    39781 ctctttattg atatcgatct ccgtttagca ggatggatat ttgaagaaaa ctgtcgtgtt
    39841 gagatagccg ttgatggtct caagcacggt tcaggaattg gttactgtga ctatgtactt
    39901 tatggtaaaa atgggaaaat tttagcgatt gtggaggcta aaaaagcctc tgtcaatcca
    39961 gaagtagggg aagtacaggt caaagaatat gctgaagctt tggagaaaca tatcggctat
    40021 cagccaattt gctttattac aaatgggttg aagcactata tacttgatgg tccgaaccgc
    40081 cgccagattg caggctttta ctctcaagaa gaattgcaat tagtgatgga tagacgtcat
    40141 cttcaaaaac cgcttgagga tatttctagt aaaattaggg acgatatttc cgggcgtcac
    40201 taccaaaaac atgccattgc aagcgtttgt gaagctttct ctgatcatcg tagacaggca
    40261 cttttggtta tggcaactgg ggcggggaaa actcgtacag cagtttctct agttgatatc
    40321 ttatcacgtc ataactgggt aaaaaacgtt ctcttcttag ccgatagaac ttccttggtt
    40381 aagcaagcat atgattcgtt tagaaaatta ctcccagatc tttccgtttg taacttctta
    40441 gaagataaag aaggagctca atcaagtcga atggtctttt caacttatcc gaccatgatt
    40501 ggagcgatta gtggtcaaga agaagtaaat caacgccctt ttactgttgg gcattttgac
    40561 cttatcataa ttgacgaatc tcaccgttct atttatcaga aatacaagtc catttttgat
    40621 tattttgatg caaggattgt aggcttaaca gctactccgc gtcaagattt agataaaaac
    40681 acctatggat tctttaattt ggagaatggg gttccaacat atgcatatga tttggaagag
    40741 gctgttaaag acggatattt agtagcctat cattctatcg aaaccaaact gaaactacct
    40801 acggatggtc tacattatga tgatttgtcc gaagaagaaa aggaacattt tgatagcaaa
    40861 tttgaagacg atagctgtga aaaagatatt gatgggagtg tgtttaattc ctttattttc
    40921 aataaaagta cagtagaaat tgttttaaat gaactcatga caagaggaat tcagacagcc
    40981 tcgggtgatg aaattggtaa aactattatt tttgctaaaa atcatgacca tgcggaatat
    41041 atcagaggta tttttaacaa ccgctatcct gaaaaaggga gcgactatgc tcaggtgatt
    41101 gattatagta ttaagcatta tcagaccttg attgatgatt ttaaaattaa ggagaagtat
    41161 cctcaaattg cgatttctgt cgatatgtta gatacaggta ttgatgtacc agaggttgtt
    41221 aatttagtct tcttcaagaa agtacgctct aaaactaagt tttggcagat gattggtcga
    41281 ggaacccgtc tatgtaaaga tttatttgga cctgagcagg ataaggaaaa cttcttggta
    41341 tttgattatg gggacaattt tgattatttt cgtgcagatc caagagatgg agagggtcgt
    41401 cacattgttt cgctgactca gcgtttattt aatatcaaag tggacttgat tcgagaactt
    41461 cagggactcc aataccaaga agatcagttt gcgagagcat accgtcagca gcttgtctcg
    41521 gaacttcaag gtcgtataga gagcttaaat gagttggact tcagggttcg tatggtttta
    41581 gatacagttt atagctatag gaaattggaa agttggcaga atctaactgc tgttacaagt
    41641 gaaaccattc aaaaaaatct ctctccgctt ttatttgatg aagataaaga agatgagatg
    41701 gcgaggagat ttgatttgtg gttgcttcat attcagttgg ggcaactgac agctaaatct
    41761 tccactgttc atatttccca agtgatgaag acggctagag ctctttctgc tattggcaat
    41821 atcccgcagg tttttgagca ggctgaaatt atggctcttt gtcaactgta gtgggttgaa
    41881 aaaaagctaa gcttgagaaa ggacaaattt
//
LOCUS       DAVBBS010000011        40583 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVBBS010000011 DAVBBS010000000
VERSION     DAVBBS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40583
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.011"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..140
                     /locus_tag="VQU32_000581"
     CDS             <1..140
                     /locus_tag="VQU32_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2472081.1"
                     /translation="RSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHELG
                     F"
     gene            343..573
                     /gene="blpU"
                     /locus_tag="VQU32_000582"
     CDS             343..573
                     /gene="blpU"
                     /locus_tag="VQU32_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HET2472082.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
     gene            1288..3441
                     /locus_tag="VQU32_000583"
     CDS             1288..3441
                     /locus_tag="VQU32_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HET2472083.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTGINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            3454..4803
                     /locus_tag="VQU32_000584"
     CDS             3454..4803
                     /locus_tag="VQU32_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET2472084.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAMETGASLASQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4974..5681
                     /locus_tag="VQU32_000585"
     CDS             4974..5681
                     /locus_tag="VQU32_000585"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET2472085.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            5883..9608
                     /locus_tag="VQU32_000586"
     CDS             5883..9608
                     /locus_tag="VQU32_000586"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HET2472086.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVTKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            9701..11143
                     /locus_tag="VQU32_000587"
     CDS             9701..11143
                     /locus_tag="VQU32_000587"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET2472087.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            11180..12202
                     /locus_tag="VQU32_000588"
     CDS             11180..12202
                     /locus_tag="VQU32_000588"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET2472088.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            12199..12744
                     /gene="purN"
                     /locus_tag="VQU32_000589"
     CDS             12199..12744
                     /gene="purN"
                     /locus_tag="VQU32_000589"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET2472089.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIALVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12828..13337
                     /locus_tag="VQU32_000590"
     CDS             12828..13337
                     /locus_tag="VQU32_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET2472090.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLIFFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            13362..14909
                     /gene="purH"
                     /locus_tag="VQU32_000591"
     CDS             13362..14909
                     /gene="purH"
                     /locus_tag="VQU32_000591"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET2472091.1"
                     /translation="MTKRVLISVSDKADIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDGVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYDDAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGENKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            15031..16293
                     /gene="purD"
                     /locus_tag="VQU32_000592"
     CDS             15031..16293
                     /gene="purD"
                     /locus_tag="VQU32_000592"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET2472092.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVSETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFFEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNITWTDKGVTLGVVVA
                     SKGYPLNYSKGVELPVKTDGDIITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            16696..17184
                     /gene="purE"
                     /locus_tag="VQU32_000593"
     CDS             16696..17184
                     /gene="purE"
                     /locus_tag="VQU32_000593"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET2472093.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            17171..18262
                     /gene="purK"
                     /locus_tag="VQU32_000594"
     CDS             17171..18262
                     /gene="purK"
                     /locus_tag="VQU32_000594"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET2472094.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            18272..18499
                     /locus_tag="VQU32_000595"
     CDS             18272..18499
                     /locus_tag="VQU32_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HET2472095.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            18562..19860
                     /gene="purB"
                     /locus_tag="VQU32_000596"
     CDS             18562..19860
                     /gene="purB"
                     /locus_tag="VQU32_000596"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET2472096.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19915..23853)
                     /gene="strH"
                     /locus_tag="VQU32_000597"
     CDS             complement(19915..23853)
                     /gene="strH"
                     /locus_tag="VQU32_000597"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET2472097.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEDDKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKESARTVNLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGSMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQTLNEVPKDLNRY
                     TAESVAAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVIEKKLPETGTHDSAGLVVAGLMATLAAYGLTKRKED"
     gene            complement(24168..24884)
                     /locus_tag="VQU32_000598"
     CDS             complement(24168..24884)
                     /locus_tag="VQU32_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2472098.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            25232..27019
                     /locus_tag="VQU32_000599"
     CDS             25232..27019
                     /locus_tag="VQU32_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HET2472099.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPVYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            27016..27492
                     /locus_tag="VQU32_000600"
     CDS             27016..27492
                     /locus_tag="VQU32_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET2472100.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            27520..28425
                     /locus_tag="VQU32_000601"
     CDS             27520..28425
                     /locus_tag="VQU32_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2472101.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            28412..29227
                     /locus_tag="VQU32_000602"
     CDS             28412..29227
                     /locus_tag="VQU32_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2472102.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            29234..29638
                     /locus_tag="VQU32_000603"
     CDS             29234..29638
                     /locus_tag="VQU32_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2472103.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            29937..31103
                     /locus_tag="VQU32_000604"
     CDS             29937..31103
                     /locus_tag="VQU32_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET2472104.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVAKPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTTLLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVVLGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            31219..32256
                     /locus_tag="VQU32_000605"
     CDS             31219..32256
                     /locus_tag="VQU32_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HET2472105.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGALLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            32464..33318
                     /locus_tag="VQU32_000606"
     CDS             32464..33318
                     /locus_tag="VQU32_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2472106.1"
                     /translation="MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREYYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            33468..33725
                     /locus_tag="VQU32_000607"
     CDS             33468..33725
                     /locus_tag="VQU32_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HET2472107.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(34101..34193)
                     /locus_tag="VQU32_000608"
                     /pseudo
     CDS             complement(34101..34193)
                     /locus_tag="VQU32_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(34229..34525)
                     /locus_tag="VQU32_000609"
     CDS             complement(34229..34525)
                     /locus_tag="VQU32_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2472108.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLISQGLTVTNVEGGMNAWPGQVE"
     gene            complement(34515..34676)
                     /locus_tag="VQU32_000610"
     CDS             complement(34515..34676)
                     /locus_tag="VQU32_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HET2472109.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMV
                     NPEGVEYGN"
     gene            34746..35510
                     /locus_tag="VQU32_000611"
     CDS             34746..35510
                     /locus_tag="VQU32_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HET2472110.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLDEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPSRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            36123..37502
                     /locus_tag="VQU32_000612"
     CDS             36123..37502
                     /locus_tag="VQU32_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET2472111.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            37516..38181
                     /locus_tag="VQU32_000613"
     CDS             37516..38181
                     /locus_tag="VQU32_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET2472112.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            38295..38390
                     /locus_tag="VQU32_000614"
                     /pseudo
     CDS             38295..38390
                     /locus_tag="VQU32_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            38536..38985
                     /locus_tag="VQU32_000615"
     CDS             38536..38985
                     /locus_tag="VQU32_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2472113.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDAFVTNFVYEKEGQSRKKSMSYESVLPVRQIFD"
     gene            39142..39552
                     /locus_tag="VQU32_000616"
     CDS             39142..39552
                     /locus_tag="VQU32_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HET2472114.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKRIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            39668..>40583
                     /gene="pavB"
                     /locus_tag="VQU32_000617"
     CDS             39668..>40583
                     /gene="pavB"
                     /locus_tag="VQU32_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HET2472115.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAV"
BASE COUNT        11241 a         7839 c         9487 g        12016 t
ORIGIN      
        1 agagaagcaa ggttgagaat atctttgcca aagtaaaaac gtttaaaatg ttttcaacaa
       61 cctatcgaaa tcatcgtaaa cgcttcggat tacgaatgaa tttgattgct ggtattatca
      121 atcatgaact aggattctag ttttgcagga agtctaatag taaaaaagtg attagaaaac
      181 atctttttta aaaatagaga tgattttgaa acaaaaaagc taattcaaga cgtttcgatg
      241 ccaattcaag atttggatga aaaaaattaa tagatactgt tatactaaac ttgtcaagtt
      301 tgtaacaaga caaatattaa aaataaaaaa gaggtattcg ttatgaatac aaaaaagatg
      361 tcacaatttg aaattatgga tactgagatg cttgcttgcg ttgaaggtgg cggatgcaat
      421 tggggagatt ttgccaaagc aggtgttgga ggaggagcag tacgaggtct tcagctagga
      481 attaaaacaa gaacatggca aggtgcagca actggtgctg cgggaggagc tatacttgga
      541 ggtgtggcct atgcagcgac atgttggtgg taattatgga ttttaaaagt tttattattg
      601 gtttagtagt tggtatattt ggtccttata tggatgattt aattagaaaa aaatttttaa
      661 agtcttcgga gaagaaaaca gaaaaatctg ttaaaaaata atcaaaacta taaatgatga
      721 atctgaatca aaattatttt gcgcatgtaa agaggagtct tatagtaacg agtcaaaaaa
      781 ggagtaacta tgaatcgtaa tttagaacgg tgttatctat tctgactagg aatagatcat
      841 accagaggta gcttagaaat agcagggaca ttagaaattg aagtaataaa taggatgtcg
      901 taagtgttac tatcaatgat ttatttgttt caagcttgcc tagggtgaca gtaaaaaatc
      961 aatttccttt caatagcata tttttagtgg gcaggactct tgttctgcct atttttttat
     1021 ccaaaaagtg cagttgggag ggagataggc tcatttggga aggaagtcca gtttttgttt
     1081 agtgattggg gtaagatagt tgttatcaga tgagtttata ctcttcgaaa atcaaattca
     1141 aaccacgtca acgtcgcctt gccgtatata tgtgactgac ttcgtcagtc ctatctacaa
     1201 cctcaaaaca gtgttttgag cagcctgcgg ctagtttcct agtttgctct ttgattttca
     1261 ttgagtatta gggaaaagga gatgaatatg aaatttggga aacgtcacta tcgtccgcaa
     1321 gtggatcaga tggactgcgg tgtagcttca ttagccatgg tttttggcta ctatggtagt
     1381 tattattttt tggctcactt gcgagaattg gctaagacga ccatggatgg gacgacggct
     1441 ttgggcttgg tcaaggtggc agaggagatt ggttttgaga cgcgagccat taaggcggat
     1501 atgacgcttt ttgacttgcc ggatttgact tttccttttg ttgcccatgt gcttaaggaa
     1561 gggaaattgc tccactacta tgtggtgact gggcaggata aggatagcat tcatattgcc
     1621 gatccagatc ccggggtgaa gttgactaaa ctgccacgtg agcgttttga ggaagaatgg
     1681 acaggagtga ctctttttat ggcacctagt ccagactata agccttataa ggaacaaaaa
     1741 aatggtctgc tctcttttat ccctatatta gtgaagcagc gtggcttgat tgccaatatc
     1801 gttttggcaa cactcttggt aaccgggatt aacattgtgg gttcttatta tctgcagtct
     1861 atcattgata cctatgtgcc agatcagatg cgttcgacac tagggattat ttctattggg
     1921 ctagtcatcg tctacatcct ccagcaaatc ttgtcttacg ctcaggagta tctcttgctt
     1981 gttttggggc aacgcttgtc gattgacgtg attttgtcct atatcaagca tgtttttcac
     2041 ctccctatgt ccttctttgc gacacgcagg acaggggaga tcgtgtctcg ttttacagat
     2101 gctaacagta tcatcgatgc gctggcttcg accatccttt cgattttcct agatgtgtca
     2161 acggttgtca ttatttccct tgttctattt tcacaaaata ccaatctctt tttcatgact
     2221 ttattggcgc ttcctatcta cacagtgatt atctttgcct ttatgaagcc gtttgaaaag
     2281 atgaatcggg ataccatgga agccaatgcg gttctgtctt cttctatcat tgaggacatc
     2341 aacggtattg agactatcaa gtccttgacc agtgaaagtc agcgttacca aaaaattgac
     2401 aaggaatttg tggattatct gaagaaatcc tttacctata gtcgagcaga gagtcagcaa
     2461 aaggctctga aaaaggttgc ccatctcttg cttaatgtcg gcattctctg gatgggggct
     2521 gttctggtca tggatggcaa gatgagtttg gggcagttga ttacctataa taccttgctg
     2581 gtttacttta ccaatccttt ggaaaatatc atcaatctgc aaaccaagct tcagacagcg
     2641 caggttgcca ataaccgtct aaatgaagtg tatctagtag cttctgagtt tgaggagaag
     2701 aaaacagttg aggatttgag cttgatgaag ggagatatga ccttcaagca ggttcattac
     2761 aagtatggct atggtcgaga cgtcttgtcg gatatcaatt taaccgttcc ccaagggtct
     2821 aaggtggctt ttgtggggat ttcagggtca ggtaagacga ctttggccaa gatgatggtt
     2881 aatttttacg acccaagtca aggggagatt agtctgggtg gtgtcaatct caatcagatt
     2941 gataaaaaag ccctgcgcca gtacatcaac tatctgcctc aacagcccta tgtctttaac
     3001 ggaacgattt tggagaatct tcttttggga gccaaggagg ggacgacaca ggaagatatc
     3061 ttacgggcgg tcgaattggc agagattcga gaggatatcg agcgcatgcc actgaattat
     3121 cagacagaat tgacttcgga tggggcaggg atttcaggtg gtcaacgtca gagaatcgct
     3181 ttggcgcgtg ctctcttgac agatgcgccg gtcttgattt tggatgaggc gactagcagt
     3241 ttggatattt tgacagagaa gcggattgtc gataatctca tggctttgga caagaccttg
     3301 attttcattg ctcaccgctt gactattgct gagcggacag agaaggtggt tgtcttggat
     3361 cagggcaaga ttgtcgaaga aggaaagcat gctgatttgc ttgcacaggg aggcttttac
     3421 gcccatttgg tcaatagcta gaaagaggag aggatgaaac cagaattttt agaaagtgcg
     3481 gagttttata atcgtcgtta ccataatttt tccagtagtg tgattgtacc catggccctt
     3541 ctgctcgtgt ttttacttgg ctttgcaact gttgcagaga aggagatgag tttgtccact
     3601 agagctactg tcgaacccag tcgtatcctt gcaaatatcc agtcaactag caacaatcgt
     3661 attcttgtca atcatttgga agaaaataag ctggttaaga agggggatct cttggttcaa
     3721 taccaagaag gggcagaggg tgtccaagcg gagtcctatg ccagtcagtt ggacatgcta
     3781 aaggatcaaa aaaagcaatt ggagtatctg caaaagagcc tgcaagaagg ggagaaccac
     3841 tttccagagg aggataagtt tggctaccaa gccacctttc gcgactacat cagtcaagca
     3901 ggcagtctta gggctagtac atcgcaacaa aatgagacca tcgcgtccca gaatgcagca
     3961 gctagccaaa cccaagccga aatcggcaac ctcatcagtc aaacagaggc taaaattcgc
     4021 gattaccaga cagctaagtc agctatggaa acaggtgctt ccttggccag tcagaatcta
     4081 gcctactctc tttaccagtc ctacaagtct cagggcgagg aaaatcccca aactaaggtt
     4141 caggcagttg cacaggttga agcacagatt tctcagttag aatctagtct tgctacttac
     4201 cgtgtccagt atgcaggttc aggtacccag caagcctatg cgtcagggtt aagcagtcaa
     4261 ttggaatccc ttaaatccca acatttggca aaggttggtc aggaattgac ccttctagcc
     4321 cagaaaatct tggaggcaga gtcaggtaag aaggtacagg gaaatctttt agacaagggg
     4381 aaaattacgg cgagtgagga tggggtgctt catcttaatc ctgagaccag tgattctagc
     4441 atggttgcag aaggtgccct actagcccaa ctttatccat ctttggaaag agaagggaaa
     4501 gccaaactca cagcctatct aagttcaaaa gatgtagcaa gaatcaaggt cggtgattct
     4561 gttcgctata ctacgactca tgatgccggg aatcaacttt tcctagattc tactattaca
     4621 agtattgatg cgacagctac taagactgag aaagggaatt tctttaaaat cgaggcggag
     4681 actaatctaa cttcggagca ggctgaaaaa cttaggtacg gggtggaagg ccgcttgcag
     4741 atgattacgg gcaagaaaag ttatctacgt tattatttgg atcaattttt gaacaaagag
     4801 taatgttcgt gtttttagag ttaaataatt tttaaactgt gagaaagatt cttcttgcag
     4861 ttttttcttt acaattttta aaaaaacatc tgctatttat tcggttaaat tcttgtgttt
     4921 tttggttttt tgtggtaaaa tgtgctcaag taatacgaaa ggcgaacttt aaaatgtcaa
     4981 aacaattgat ctattcggga aaagctaaag atatctatac aactgaggat gaaaatctta
     5041 ttatttcaac ttacaaggac caggcgactg ctttcaacgg tgtcaagaag gagcagattg
     5101 caggtaaggg agtcttgaat aatcagatct catcttttat ttttgagaaa ttaaatgcgg
     5161 ctggtgtggc gactcacttt gtggagaaac tttcagacac ggaacaactc aataaaaagg
     5221 ttaagattat tcctttggaa gtcgtgctcc gcaactatac tgctggttcc ttttcaaaac
     5281 gttttggtgt ggatgaggga atcgccttgg agactccgat tgtcgaattt tactacaaaa
     5341 atgatgattt ggatgatcca tttatcaatg atgagcatgt gaaattccta cagattgcgg
     5401 gtgaccagca gattgcctac ttgaaggaag aaacgcgtcg tatcaatgaa ctattgaaag
     5461 tctggtttgc tgagattggg cttaaattga ttgactttaa gctagagttc ggttttgaca
     5521 aggatggcaa gattatcttg gcagacgaat tttcaccaga taactgccgc ttgtgggacg
     5581 ctgatggcaa ccacatggat aaggatgttt tccgtagagg attgggagaa ctaaccgacg
     5641 tttatgagat tgtttgggaa aagttgcagg aattgaaata atctgtttgc aacggaaaac
     5701 cttcgtctct caactaaaag gactcaggct gaaaaggtcc cccagacctt ttcactctgt
     5761 agagaactag gtgaactaac agatgtttac gaaattgtct gggaaaagtt gcagggttta
     5821 aaataacaac ctcaaggctg tttgggaata ttgcaagagc tgaaataaag gaataagaat
     5881 tgatggataa acgtattttt gttgaaaaaa aggctgattt tcaggtcaag tcagagagtt
     5941 tggttagaga gctccagcac aacttgggac tgtcaagctt gaaaagtatt cgtattgtgc
     6001 aagtatatga tgtatttgac ttggctgagg acttgtttgc acctgcagag aagcacattt
     6061 tctctgagca gggaaccgac catgttttag atgaagtatc tgtgcaggcg gatcttgcta
     6121 actatgcttt ctttgccatt gaaagtctgc cagggcagtt tgaccagcgt gcagcttcgt
     6181 cacaggaagc cttgcttttg ttgggaagtt cgagtgacgt gacagtcaac acagcccaac
     6241 tttacttggt gaataaagat attgatgcga ctgagttgga agctgtcaaa aactacctgc
     6301 tcaatccagt tgattctcgt ttcaaggata tcacgacagg gattgccaag caggagtttt
     6361 cagagtcaga caagaccatt ccaaaattga ctttctttga aagctataca gcagaagact
     6421 ttgctcgcta caaggccgag caagggatgg ccatggaagt ggatgatttg ctctttatcc
     6481 aagactactt taagtcaatc gggcgcgtgc caactgagac tgaactcaag gttttggaca
     6541 cttactggtc tgaccactgc cgtcatacga cttttgagac agagttgaaa cacatcgact
     6601 tttcagcttc taaatttcaa aagcaattgc agtcaaccta tgacaagtat attgccatgc
     6661 gcgaggagtt aggtcggtct gaaaaaccac aaaccttgat ggatatggcg actattttcg
     6721 gtcgttatga gcgtgctaac ggacgattgg atgatatgga agtctctgac gaaatcaatg
     6781 cctgctcagt tgaaattgaa gtggatgttg atggtgtcaa ggaaccttgg ctcctcatgt
     6841 ttaaaaatga aacccacaac catccaacag aaattgagcc atttggtgga gcggctacct
     6901 gtatcggtgg agctattcgt gatccgttgt caggccgttc ctatgtttac caagccatgc
     6961 gtatttcagg tgctggtgat attacagcac cgatttcgga aactcgtgct gggaaattgc
     7021 cacaacaagt catttctaaa acagcagctc atggttattc ttcatatggt aaccagattg
     7081 ggcttgccac aacctacgtt cgtgaatact tccacccagg ctttgtagct aaacgtatgg
     7141 aacttggtgc cgttgttggt gcggctccca agggcaatgt tgtccgtgaa aaacctgaag
     7201 caggtgatgt gatcatcctt ctcggaggca aaacaggtcg tgatggtgtc ggtggtgcga
     7261 cgggctcttc taaggttcaa acagttgagt ctgtagagac tgctggtgct gaggttcaaa
     7321 aaggaaatgc catcgaagaa cgcaagattc agcgcctctt ccgtaatggc aatgtcactc
     7381 gtctgatcaa gaagtccaat gactttgggg caggcggcgt ctgtgtggct atcggtgaat
     7441 tggcagacgg tcttgaaatc gacctcaaca aggtgcctct taaataccag ggcttgaatg
     7501 gtacagaaat tgccatctct gaatcacaag aacggatggc ggtcgtggtt cgtcctgaag
     7561 atgtggatac cttcgttgcc gaatgtaaca aagaaaatat tgatgctgtt gtggtggcga
     7621 cagtaactga aaaaccaaat cttgtcatgc actggaatgg tgagacaatc gttgacttgg
     7681 agcgtcgttt ccttgacacc aatggtgtgc gcgtggttgt cgatgccaaa gttgtggaca
     7741 aggatgtcaa actcccagaa gagcgtcaaa catctgctga aacactggaa tcagataccc
     7801 ttacggttct atctgacctc aaccatgcaa gtcaaaaagg attacagact atctttgact
     7861 gctctgttgg acgctcaacg gttaatcacc cacttggtgg tcgttaccaa ctcacaccaa
     7921 ctgaggcatc tgtgcagaaa ttgccagttc aacacggtgt gactcatact gcgtcggtca
     7981 ttgctcaagg tttcaaccca tatgtagctg aatggtctcc ataccacggt gctgcttatg
     8041 cggttatcga agcaactgct cgtttggtgg ctgctggtgc caactggtct aaggctcgtt
     8101 tctcttacca agaatatttc gagcggatgg acaagcaagc agagcgtttc ggtcagccag
     8161 tagctgctct tctaggttct attgaagcac aaattcagct tggcttgcca tctatcggtg
     8221 gtaaggactc catgtctggt acctttgaag aattgaccgt tccgccaacc ttggttgcct
     8281 ttggggtgac gacggcagat agccgtaagg tgctctctcc agaatttaaa gctgttgggg
     8341 aaaatatcta ctacatccca ggtcaagccc tctctgcaga gattgatttt gacttgatta
     8401 agaaaaattt tgctcagttt gaagcaagcc aagctgacca taaagtgacg tctgcatcag
     8461 ctgtcaaata cggtggtgta gttgaaagtt tggctcttgc tacctttgga aactatattg
     8521 gtgcagaggt gaccttgcct gaacttaaaa cagctttgac agctcaatta ggcggctttg
     8581 tcttcacatc tcctgaagaa attgctggag tagagaagat tggacaaacg aaagcagact
     8641 ttacactgac tgtcaacggt gtgaagctag atggacacaa gcttgacagt gcatttcaag
     8701 ggacattgga agaagtttac ccaacagaat ttacccaagc gaaagaacta gaagaagtac
     8761 cagctgtggc atcagatgtt gtgattaaag ccaaagaaaa ggttgaaaaa cctgtggttt
     8821 acatcccagt ctttccagga accaactcag aatatgattc agctaaggcc ttcgaaaaag
     8881 aaggtgcaga ggtcaatttg gtgccattcg tgaccttgaa tgaagaagct attgtcaagt
     8941 cagttgaaac tatggttgac aatatcgaca agactaatat tctcttcttt gccggtggat
     9001 tctcggctgc ggatgaacca gatggttcag ctaagtttat cgtcaatatc ctgcttaatg
     9061 aaaaagtgcg tgtggctatt gatagcttta tcgcccgtgg tggcttgatt atcggtattt
     9121 gtaatggatt ccaagcctta gtcaaatcgg gtctcctacc atacggaaac tttgaagatg
     9181 ctaacagtac tagtccaacc ctcttctaca atgatgccaa ccaacacgtg accaagatgg
     9241 tggaaactcg cattgccaat accaactcac catggttggc tggtgtgcaa gtgggcgata
     9301 tccacgctat tcctgtttcg cacggtgaag ggaagtttgt cgtgacggct gaggaatttg
     9361 cagagctccg tgacaatgga caaattttca gccaatacgt tgactttaac ggtaaaccaa
     9421 gtatggattc taagtacaat ccgaatggtt ctgtccatgc catcgaagga attaccagca
     9481 agaatggtca aatcatcggt aagatgggcc actcagaacg ttatgaggat ggtcttttcc
     9541 aaaatatccc aggcaataaa gaccaacacc tgttcgcatc agcggttaaa catttcactg
     9601 gaaaataaga cttacagatt ttctaataga tagtatcagt aatgtaaaag tcatgtaaat
     9661 ctagctcttg atgattacaa atgaaaatta ggtataaaaa atgacatacg aagtaaaatc
     9721 tcttaatgaa gaatgtggtg ttttcggtat ttggggacat ccagatgctg ctaagttgac
     9781 ctattttgga ctccacagtc ttcaacaccg tggtcaggag ggggcaggaa tcctctccaa
     9841 tgatcaagga caactgaagc gccatcgtga catggggctt ttatcagaag ttttcagaaa
     9901 tccagccaat ttggataaat tgacaggagc tggtgcgatt gggcatgtgc gttatgcgac
     9961 tgctggcgaa gcttctgttg ataacatcca gcccttcctc ttccgttttc acgatatgca
    10021 gtttggcttg gctcataatg gaaatctgac caatgcagcc tctctcaaga aagaactgga
    10081 acaaagagga gcaattttca gcgcgacttc ggactctgaa atcttggctc acctcattcg
    10141 tcgtagtcat aatcctagcc tgatgggcaa aatcaaggaa gcgctcagcc ttgtcaaagg
    10201 tggttttgcc tatatcttgc tgtttgagga caagttgatt gcggctcttg accctaatgg
    10261 ctttcgtccg ctttctatcg ggaaaatggc caacggagcg gtggttgttt cctctgaaac
    10321 ctgtgctttt gaggtcattg gtgccgagtg gattcgtgat ttgaagccag gtgagattgt
    10381 gatcattgat gacgagggca ttcagtatga cagctataca gatgatactc agttggcgat
    10441 ttgttctatg gagtatatct actttgctcg ccctgattct aatatccacg gtgtcaatgt
    10501 ccatacggca cgtaagagaa tgggagcgca attggcgcga gaatttaagc atgaggcaga
    10561 tattgtagtt ggtgtgccca attcttccct aagcgcggct atgggatttg cggaagaatc
    10621 aggcttacca aatgaaatgg gtctgatcaa aaaccaatac acccagcgaa cttttatcca
    10681 accgactcaa gaattgcggg agcaaggagt gcggatgaaa ctgtctgctg tttcgggtgt
    10741 tgtcaaaggc aaacgtgtgg tcatggtgga tgattccatt gtacgtggaa caacctctcg
    10801 tcgtatcgtt cagctcttga aagaagcggg tgcgactgag gttcacgttg ccattggaag
    10861 tcctgcacta gcgtatccat gtttctacgg gattgatatc cagacccgtc aggagctgat
    10921 tgcagccaat catacggtcg aagaaactcg ccaaatcatt ggtgcggaca gtctgactta
    10981 tctttcaatt gatggcttga ttgagtcgat tggtatcgaa acagatgcgc cgaacggtgg
    11041 tctctgtgtc gcttactttg acggtgacta cccaacgcct ctctatgact acgaagaaga
    11101 ctatcgtaga agtttggaag aaaagaccag tttttacaag taggcgacag attctccatt
    11161 aaagaaaagg aaaaaacaaa tgacaaataa aaatgcatat gcccagtcgg gtgtggatgt
    11221 tgaagcgggt tatgaagttg ttgaacggat taaaaagcac gtggcccgta cggagcgtgc
    11281 aggtgtcatg ggagctcttg gtggctttgg tggtatgttt gacctttcca agactggggt
    11341 taaagaaccc gtcttgattt cagggactga cggtgtcgga accaagctca tgttggctat
    11401 caagtacgac aagcacgata ccatcgggca ggactgtgtg gccatgtgtg tcaacgacat
    11461 cattgctgca ggtgcggaac ccctctattt tctcgactac gtagcgacag ggaagaatga
    11521 accagctaag ctagaacaag tggttgctgg tgtggcagaa ggttgtgtgc aggctggtgc
    11581 tgccctcatc ggtggggaaa cggctgaaat gccgggcatg tacggcgaag acgactatga
    11641 cttggctggt tttgcggtcg gtgtggctga aaaatctcaa atcattgacg gttcaaaggt
    11701 ggtagaggga gatgttcttc tcggacttgc ttcaagtggg attcactcaa atggttactc
    11761 tttggttcgt cgtgtctttg cggattacac aggtgaggaa gtcctaccag aattggaagg
    11821 caagaaactt aaggaagttc tacttgagcc gactcgtatc tatgtcaagg ctgtcttgcc
    11881 gctcatcaaa gaagagttgg tcaacggcat tgcccacatc acaggtggtg gctttatcga
    11941 aaatgtccct cgtatgtttg cagatgacct agctgctgaa attgatgaaa gcaaagttcc
    12001 agtgcttcca attttcaaag cccttgaaaa atatggtcag attaaacacg aagaaatgtt
    12061 tgaaatcttc aatatgggtg tgggacttat gttggcggtc agccctgaaa atgtagagcg
    12121 tgtaaaagaa ttgttggatg aagcagtcta tgaaattggt cgcatcgtca agaaagaaaa
    12181 cgaaagtgtc attatcaaat gaaaaaaata gcggtttttg cctctggtaa tggctcaaat
    12241 tttcaggtga ttgccgaaga atttccagtg gagtttgtct tttcagacca tcgtgatgcc
    12301 tatgtgcttg agcgtgcaaa gcagctcggc gttctgtcct atgcttttga actcaaggag
    12361 tttgagagca aggcagacta cgaagcagcc cttgtcgaac tcttggaaga acaccagatt
    12421 gccttggttt gcctagcagg ctacatgaaa atcgttggac caaccttatt gtcggcttat
    12481 gaaggtcgga ttgtcaacat tcatccagcc tacttgccag aatttccagg agctcatggg
    12541 attgaggatg cttggaatgc tggcgtgggt cagtctggtg tgaccattca ctgggtggat
    12601 tcgggtgtgg atacaggcca ggtcatcaaa caggttcgtg tgccacgact agctgatgat
    12661 accattgaca gatttgaagc tcgcatccat gaagcagagt acaggttgta tccggaagta
    12721 gtgaaggctc tatttacaga ttgacttttt gatgattcat atgatatctt tgattttaaa
    12781 ttggagtcag tgtttgttga agacggcttc aaacggaggt atttgtaatg ttagaatcta
    12841 aaaaaacaac tcgatatgta ttttatgtct atctgatgtt attaacttgg ggaatcttat
    12901 ttaagtttga aacaaatcct gaatttatag catttttctt agctccaagg tatatcaatt
    12961 ggattccatt ttcagaacca ctaatagtcg atggaaaaat tgtttttgct gaaatgttat
    13021 ttaatctgat tttctttatt ccattaggtg tttgtttccc tttgataaaa actaatttat
    13081 ctagtttaag aatagtcggg acaggtttct tgattagttt attgtttgag tgcttacagt
    13141 atattttagc aataggtata acagatataa cggatttgac tttaaatacg ctaggtgtct
    13201 gtgtaggctt actgatttat caaattttta taagagtgtt caaatcacag actagaaaat
    13261 ggatcaatat cttaggtatg cttagccttg gttttgctta tcttgtttta ctgttactgc
    13321 atttaattgg tgtttaacta atgattaaaa aggagaatat aatgactaaa cgcgtcttaa
    13381 tcagcgtctc agacaaagcg gacattgttg aatttgccca agaactcaaa aaacttggtt
    13441 gggagattat ctcgacaggt ggaactaagg ttgcccttga taatgctggg gtggatacca
    13501 ttgctatcga tggtgtgact ggtttcccag aaatgatgga cggtcgtgtg aagaccctcc
    13561 acccaaatat ccacggaggg cttctcgctc gtcgtgactt ggatagccac ttggaagcgg
    13621 ctaaggacaa caagattgag ctcattgacc ttgtggtggt caacctttac ccatttaagg
    13681 aaactatcct taaaccagat gtgacttatg atgatgcagt tgaaaatatc gatattggtg
    13741 ggccatctat gcttcgttca gcagcgaaaa atcatgccag tgttacagtt gtggtagatc
    13801 ctgctgacta cgctgtggtt ttggacgaat tggcagcaaa cggcgaaacc tcttatgaaa
    13861 ctcgccaacg tttagcagcc aaagtatttc gtcacacagc ggcttatgac gccttgattg
    13921 cagaatactt cacagctcaa gtgggtgaaa acaagcctga aaaactcact ttgacttatg
    13981 acctcaagca accaatgcgt tacggtgaga atcctcaaca agacgcggac ttttaccaga
    14041 aagctttgcc tacagactac tccattgctt cagccaaaca gctcaacggg aaagaattgt
    14101 catttaataa tatccgtgat gcagatgctg ctatccgtat catccgtgac ttcaaagata
    14161 gtccaaccgt tgtggctctc aaacacatga atccatgtgg aattggtcaa gctgatgaca
    14221 tcgagactgc ttgggactac gcttatgagt ctgacccagt gtctatcttt ggtgggattg
    14281 tcgtcctcaa ccgtgaggtg gatgctgcga cagctgagaa gatgcacggc gttttcctcg
    14341 aaatcatcat tgcaccaagc tatacggatg aagcgctagc cattttgatc aataaaaaga
    14401 aaaacttgcg tatccttgcc ttgccattta atgctcaaga ggctagcgaa gtggaagcag
    14461 aatacacagg tgtagtcggt ggacttctcg tgcaaaatca agacgtggtc aaggaaagcc
    14521 cagctgactg gcaagtggtg actaaacgtc agccaactga gacagaagcg actgctcttg
    14581 agttcgcttg gaaggctatc aagtacgtca aatcaaatgg tattatcgtg accaacgacc
    14641 acatgacact tggtgttggt ccaggtcaaa ccaaccgtgt ggcttctgtt cgccttgcca
    14701 ttgaccaagc caaagatcgt ctggacgggg cggtccttgc ttcagatgcc ttcttcccat
    14761 ttgcggataa cgtggaagaa atcgccaaag caggaattaa ggccatcatc cagcctggtg
    14821 gctctgtccg tgaccaagaa tccatcgaag cagcggataa atacggcttg actatggtct
    14881 ttacaggtgt gagacatttt agacattaag aagataaaag ggaagaaaac agtttctttc
    14941 cttttttggc ttaaaatgct aactgaaaca agattaaaac gaactttttt gatataatgt
    15001 tggtaaataa ttcgcaaaag aggttgagga atgaaactgc ttgttgtcgg ttctggtggt
    15061 cgtgagcatg cgattgctaa aaagttactt gaatcaaaag acgtggaaaa agtctttgta
    15121 gctcctggga atgatgggat gactctggat ggtttggaat tggtaaatat ctctatttcc
    15181 gaacattcta aattgattga cttcgcaaag accaatgatg ttgcttggac ctttatcggt
    15241 ccagatgatg cccttgctgc tggtatcgtg gatgatttta accaagctgg gcttaaggcc
    15301 tttggtccga ctagggctgc agcggagctg gagtggtcca aggattttgc caaggaaatc
    15361 atggtcaaat atggtgtttc gacagcagcc tatggcacat tttcagattt cgaggaagca
    15421 aaggcctata tcgaaaagca tggtgcgcct atcgtagtca aggcggatgg cttggcgctt
    15481 gggaagggtg tcgtcgtttc tgagacggtt gagcaagcgg tcgaagccgc tcatgagatg
    15541 cttttggaca ataaatttgg tgactcaggt gcgcgcgtgg ttattgagga attctttgaa
    15601 ggagaggaat tttcactctt tgcctttgtc aatggtgata agttctacat catgccaacg
    15661 gctcaggacc acaaacgtgc ctatgatggc gacaaagggc ctaacacggg tggtatgggt
    15721 gcctatgcgc cagttccaca cttaccacag agtgtagttg atacagcggt tgacaccatt
    15781 gtcaagccag ttctagaagg gatgattaaa gaaggtcgcc cttatctggg agttctttac
    15841 gcagggctta tcctgacagc tgatggaccg aaagtcattg agttcaacgc tcggttcgga
    15901 gatccagaaa ctcagattat cttgcctcgc ttaacctctg actttgctca aaatatcaca
    15961 gatatcctgg atagcaagga gccaaatatc acgtggacgg acaagggtgt gactctgggt
    16021 gtggttgtcg catccaaggg ctacccgcta aattattcaa agggtgttga gttgccagtc
    16081 aaaaccgatg gcgacatcat tacctactat gcaggggcta agtttgcgga aaatagcaga
    16141 gcactgctct caaacggcgg acgagtttat atgctcgtta ccacagcaga taccgtcaaa
    16201 gaagcccaag ccagcatcta ccaagaacta tcccaacaaa aaatagaagg actcttctac
    16261 cgaacagata tcggaagcaa ggcaattaag taaagatata agaataacgc gccgtagtcg
    16321 ccaaacacga taatggtcgt cgtggtgaaa agaccagaac agtgaatgtt ctggtcaggg
    16381 ggaaacttgg agaccgtagg ctcaaagttt aggaatgaaa ccgaaggttt gcttccgcct
    16441 ccatcaccta agaccattat caaaaagaaa aataaaaatt cacaaaatac gttaatgatc
    16501 gtatggtttg cgagcgttag cgagctaata tagaacaatc accgccgttg tgaaagaacg
    16561 attggatgat aatccaatcg ttcagggaaa ttggaagacc ttgggtttcc aatttaggca
    16621 tgagacacct ttggtggctg ctgccgtccc tcacaagcta aggtgattgt tgaaaaagag
    16681 gaaaaaggag aagaaatgaa accagtaatt tccatcatca tgggctcaaa atccgactgg
    16741 gcaaccatgc aaaaaacagc agaagtccta gaccgcttcg gtgtagccta cgaaaagaaa
    16801 gttgtttccg cacaccgcac accagacctc atgttcaaac atgcagaaga agcccgtagt
    16861 cgtggcatca agatcatcat cgcaggtgct ggtggcgcag cgcatttgcc aggcatggta
    16921 gctgccaaaa caacccttcc agtcattggt gtgccagtca agtctcgtgc tcttagtgga
    16981 gtggattcac tctattctat cgttcagatg ccgggtgggg tgcctgttgc gaccatggct
    17041 atcggtgaag ctggagcgac taacgcagct ctctttgccc tccgtctcct ctctgtagaa
    17101 gataagtcca ttgcggatgc acttgccaac tttgctgaag aacaaggaaa aatcgcagag
    17161 gagtcgtcaa atgagctcat ctaaaacaat cggaattatc ggtggcggtc aactgggtca
    17221 gatgatggcc atttctgcta tctacatggg ccacaaggtt atcgcgctgg atcctgcggc
    17281 ggattgcccg gcctctcgcg tggcggaaat cattgtggca ccttataacg atgtggacgc
    17341 cctccgtcag ttggcagacc gttgcgatgt cctcacttat gagtttgaaa atgtcgacgc
    17401 tgacggtttg gatgccgtta tcaaggatgg acaactccct caaggaacag atctgctccg
    17461 catttcgcaa aatcgtattt ttgaaaagga ctttttgtca aacaaggctc aagtcactgt
    17521 ggcaccctac aaggtcgtga cttctagcct agacttggca gatatcgact tgtcgaaaaa
    17581 ctatgtcctc aagactgcga ctggtggcta cgatggtcat ggacaaaagg ttattcgttc
    17641 agaagcagac ttggaagcag cctatgcgct agcagactca gcagactgcg tcttggaaga
    17701 atttgtcaac tttgaccttg agatttctgt catcgtgtca ggaaatggca aggaggtgac
    17761 gtttttccca gttcaggaaa atatccaccg caacaatatc ctgtctaaga ccatcgtacc
    17821 agcccgcatt tctgaaagtc tagcagacaa ggctaaagct atggcagtgc gaatcgcaga
    17881 acaactcaac ttgtctggaa ctctctgtgt ggaaatgttt gcgactgatg atgacgttat
    17941 tgtcaatgaa atcgccccac gaccacataa ctctgggcac tattctattg aagcctgtga
    18001 tttctctcag tttgacaccc atattctggg tgttctcgga gcaccattac cagtcatcaa
    18061 actccatgcg ccagccgtta tgcttaatgt cctcggtcag catgtcgagg ctgctgagca
    18121 atatgtgaca gaaaattcaa gcgcccacct ccacatgtat ggtaaaatag aagcgaagca
    18181 caaccgcaag atgggacatg tgaccttgtt tagtgatgta ccggatagtg tggatgagct
    18241 tggggaaggg attgattttt aggacaagtc tatgatacaa attatcgtta atacatttat
    18301 tgaaaaggat aagactggag cagttgttga agtgttgtat gccagtgctg accaagataa
    18361 ggtacaagct aaatatgaag aactagctgc acaatacccc gaaaattatt tagctatcta
    18421 tgatgtaccg ctggatacgg atttgaatac actagatcat tacccgtctg tgtttattga
    18481 aaaagaggag tttgagtaga aatcttggtt tacctagata gcttattccc aacagcttaa
    18541 gaagaaagga aaaattaaca catgatcaac cgttactctc gccctgagat ggcgaatatt
    18601 tggagtgaag aaaataaata ccgtgcttgg cttgaggtgg aaatcctctc tgacgaggca
    18661 tgggctgagt tgggggaaat ccctaaggaa gatgtggctt tgattcgcaa gaaggcggac
    18721 tttgacatcg accgtatttt ggaaattgag caggagacgc gccacgatgt ggtggctttc
    18781 acgcgtgcgg tttctgagac tcttggtgaa gagcgcaagt gggttcacta tgggttaact
    18841 tctaccgacg tggtggatac tgcttatggt tacctctaca agcaggccaa cgacatcatc
    18901 cgtcgtgacc ttgaaaactt cactaatatc atcgctgaca aggccaagga gcacaagttc
    18961 accatcatga tggggcgtac tcatggtgtg cacgctgagc cgacaacctt tggtcttaaa
    19021 ttggcaactt ggtacagcga aatgaaacgc aatatcgagc gtttcgagca tgcagctgct
    19081 ggtgtagaag ctggtaagat ttctggtgcg gttgggaact ttgccaatat cccaccattt
    19141 gtagaggagt atgtctgcga taaacttggc atccgtgccc aagaaatctc tacacaagtc
    19201 cttcctcgtg accttcacgc tgagtacttt gcggttcttg ccagcattgc gacttcaatc
    19261 gaacgtatgg cgactgagat tcgtggtcta caaaaatctg agcaacgcga agtagaagag
    19321 ttctttgcta aagggcaaaa agggtcttca gcaatgcctc acaaacgcaa cccaatcggt
    19381 tctgaaaata tgactggtct ggcgcgtgtc attcgtggtc acatgattac ggcttatgaa
    19441 aacgtcgctc tctggcacga acgcgatatt tctcactcat cagctgagcg tatcatcaca
    19501 ccagatacga ccattttgat tgactacatg ctcaaccgtt ttggaaatat cgtcaagaac
    19561 ttgacagtct tcccagaaaa tatgatccga aacatgaact cgacttttgg tcttatcttt
    19621 agccaacggg ctatgttgac attgattgaa aaaggcatga cccgtgagca agcctatgac
    19681 ttggtgcaac caaaaacagc ctactcttgg gacaaccaag tagactttaa accacttctt
    19741 gaggcagatt cagaagtaac atcacgtctc actcaagaag aaatcgatga aatcttcaac
    19801 ccagtttatt acaccaaacg agtggatgat atctttgaac gtcttggact aggtgattaa
    19861 ttaaaaaata aacagcgagc ttcaatctcg ctgtttattt tttatcgaaa agacttagtc
    19921 ttcttttctt ttagtgagtc cataggctgc tagtgtggcc atgagtcctg cgactactag
    19981 tcctgcagaa tcgtgagttc ctgtttcagg aagttttttc tctattacca caggagctgg
    20041 atcttgagga agagctttgc tttcctcagc aggagcagtt gatggagctg gttggcttgg
    20101 gatttctagt tttggttttt cttcagcaat agcggcttgt ccgttttcat cgcctacatg
    20161 tgttaccata gttccgactt cgactatttg agtaacggct tcctgtgcta cgacactatt
    20221 tacaagtgtt ttcacttcct taccatcggc agaagtgctc acagagtaga agttgctacg
    20281 atgtccattg acgcccttag taatgacttg tgtttttcct ttgagtaaga gtggattttc
    20341 acgagtcact gtggtaaatg gaatttcttc tttttggata tccagtctag gttttacctc
    20401 agtagttggt gcaagaccac tttcatcacc cttgtgagtt acaggagcgc caacttcaac
    20461 cacttggttt ataacttctt tggttacctg gctatcaagg actgtttctg ttgtttttcc
    20521 attttcagtg agtacagaga tgtaatgagt tcgctcacct ttgactcctg ctgtgataat
    20581 attttcctga ccagctggga ggttaggatt ttctttcttg ataacttcaa atggaatttc
    20641 ttcagttctt gtgatgagtt ctggtctggt ttcaacattg gcagccactt cattttcatc
    20701 caggcttcct gaatgagttg cagctggttt gaggccttga agagcggctt ttaggttggc
    20761 tacaagtgtg tcaagctcag cttgtttatt acggttgagg ttgtaattta gagctgtttt
    20821 agctgcgtca agggcctcaa gactttcttt actatatcct tctaagtttg taggaatttt
    20881 agctaattct tcgcggagag cattataatt agcacggaag tagtctttgt tgtggtctgc
    20941 aaaggcagtc atgagttcaa agatttcctc ttccttgtat tcagcgcttg gtctatctgc
    21001 ccagattgaa agcatacttc cgactgttgg aagatctact tcaggatatt tggtagaagc
    21061 tagttgattg aatggtgttt ttccagtatt ctcaatagct ttcttgagga aaccaccacc
    21121 atcttctggt ttttgaccaa gaatgtagta ccagtcaccg ttggtattca agaatttata
    21181 gcctttgctt gctaggtatt gaggtgatgc gaggttatat ccccaccagc ctttagacca
    21241 gtaagaaatc aagacatctt tgtcaaactg aacatcgtcc ttgtcttcat agtagaagcc
    21301 atcgttgaag gccattggtt gaagccctct ttctttggcc atagctgcga gggtgttggc
    21361 atattcggca aatttgccat agagttgata ccacttgagg tagtaccagc cttgggcact
    21421 agtcgcatcg ttggcgtatt cgtcagtacc aaagttgaaa atctttgttt tacctgcaaa
    21481 gaagtccatg tatttaccga tgagggcttt tacaaagttc atcgcttctt cgtttttcaa
    21541 gtccatagtt gtttttgaaa ctttatcaaa gtgggcttga ggatttttaa tacctaattt
    21601 ttccatggca accagcatag catccatgtg acctggactg ttaatagctg ggatgagacc
    21661 gatgtcctta gatttagcgt attcaattag ctctgttact tctgcctgtg ttagtgcagt
    21721 accgtttgga tcgtcgtagt aagctttagt tccttcgata atagcttttt taacgtcatc
    21781 actagcatag gtttttccgt tggcagtaat ggtcatatca tcgagtagaa agcgaagtcc
    21841 gtcatttcct agaaggagat ggacatcaga atatccgagc tcactggcct tgtctacgat
    21901 gcgtttgagc tggttcagag taaagtattt gcgtccagca tcgattgaga ttaccttgtt
    21961 tttggcaagt ttttcaacct cacgtttagc ttcttcttct ttttgagctt caggcgtgag
    22021 ggtcaagttg ttgacagttt cttgaagttt agcaatggct tgatcaatcg tatcttgttg
    22081 ggcacggcta aggttgctat cgagagagcg aatagctttt tcagcttctt ttacggccgc
    22141 gacgctttct gcagtataac ggttcaggtc ttttggtacc tcgttaagtg tttgctctgc
    22201 agattcataa tcagctgcga agtattcagc gttggcattt gcaaaatgac gcatgagttt
    22261 gaagaggcgt gatggtgaat aacgtgcaga tggagtgtca gcccaagcag ctaccatact
    22321 accgatgatt gggatatcag ctccttctgt ttttggtaca gaagtgattg gtgtgttttt
    22381 aataccattg agcccctgat cgagattgta ccagccttgg ccatcagcgt ttcgtccaag
    22441 aacgtagtac caagcatcat tggtattaag gatttggtga cctttttcag ctagtagttt
    22501 agaagaagcg acatcgtagc ctccccaacc accagtccac atagaaacga tgatgtcttt
    22561 gtcaaaacta ccaaagcttg tgtcgctatt gtagtagata ccgtcgttaa aggccattgg
    22621 tttgagaccg tgcgatttta caatacgagc gaggtcattg gcgtaggcaa taaatttttc
    22681 atagcctttt acagggtagc cttcgtttgg atagtattta tcagcttgaa gcacactcca
    22741 acctttagca tctgtcgcat cattggcata ttcatcaagt ccgatgttga agatttcagt
    22801 ctttttcgcg aaataagcag catacttgtc gataagggct tttgtaaaag cgacagcttg
    22861 ttcgttgtca agattgacag tacgggctga ttccttccca aaatagctaa agttagggtt
    22921 ttggattccc aattctttca tggcattgag aatcgcatcc atgtgaccag gactatttac
    22981 tgtcggaatg agaccgatac ctttatcttt ggcatagtta atcagatctg tcatttgact
    23041 ttctgttaag tgattgccgt ttggatcgtt gtaataatca tttgtacctt tttcaatggc
    23101 gcgtttgaca tcgtcactgg cataggtctt gccgttagct gtgatgctca tatcgtccaa
    23161 catgaaacgg agtccatcat ttccgactaa taggtgtaaa tcagtgtagc cataatgttt
    23221 cgctttatcg atgatttcct tgagctgttc tggtgagaaa tatttacgtc cagcatcaat
    23281 agaaacaatt ttctttttcg ctagtttttc atttacagtt gcagcacgtt cctttcctgc
    23341 ctctgtttcc ggtttgtcat cctctgcttt cgcttcatct tttttagctg gtttatcctt
    23401 gtcagtcttg tctgtatttg actctttaga atcaacctct ttcgcttctt cctttttagg
    23461 gctagcttct tctgcctttt tattagcagt ttctttttca gcagaagttg gagttaccac
    23521 ttctgcttta tcactaggag ttgaactaac ttcctcttgt ggtttttctt ctgtttttgg
    23581 aagactagct accttatcag tagctggagt ttctgtttct acagtttttg gagcttctgg
    23641 ttgaagctct gctttaggtg tttcctcagt ccgattttcg gatgattgag gggaatcaga
    23701 aaccgtatgg atggtcggtt ggttttctgt agtagtagga gtaactccat cggctgcaac
    23761 agtctgtgct tggaaggcaa atccaattag aacagaagct gctcctacag cgtatttacg
    23821 aatagaaaaa cgctgttgtt tttcatgttt cattgcaaaa cctcctgatt gcattgttat
    23881 attgatagcg attatataaa tcaacgcctt tattttattt cttatattaa cgagagtcaa
    23941 gaggagatga caaaaaacta taataagtat aaaaaaatat aaaatttaaa cttaagattt
    24001 cagattggtc ggaaaaaata cgtatatata tctagtataa tttttggttc tatttctata
    24061 aaatattcca caaattatag aattttccaa aaataggtaa gcgctacctt tttggtgtgg
    24121 tataataagc atagaaaaag cccaagcgat tagctcaggc tttcttctta gtgatcacgg
    24181 tcacatgaga taaatttaat cttgtagtaa tcagatcgtt tgtaagtttc actgtattct
    24241 aaaacttggc cagttgattc gagtttggtg attttagttt gtaggacagt agggaattgt
    24301 tcatcgactc cgaggactga agccgcatgt tctggagttg gaaagactat ttcgttgatt
    24361 tcttcaaagt gttcatcatt catgtgaatg tggtagtcta acttgaaacg attatagata
    24421 gaactatagt attcaaggtt tggataattt gcgttgatat attgttctgg gatgtaggat
    24481 gtatggtaga tataaacgac accgtttgat tcgcggatac gttcaatctt gtagtagaat
    24541 tgatcgccgc gtagacccaa tttttccaag taaacaagct tgtttccgcg ttcaattgaa
    24601 agaacagtta ccttatcatc tttagcattg aagagttcaa tatctgaaaa ctctacaagc
    24661 ttgtgtttgc gtgcacgtga aacgaaggtt ccttttcctt gttggcggac aatatagcca
    24721 tctttggcaa ggtcgtttaa ggcgcgaaca actgtgatag agctgacatc gtacattgaa
    24781 atgagttctg cttcagtgta aaatttatct ccactgctaa actgcccaga gatgatttta
    24841 ttttttaatt cgtcttttat gtattgatat ttgggaattg ccatattttt acctctattt
    24901 tccttctcca taggaaattt taccataaaa gcgaaaaaaa tagtagtctt ttgaataaaa
    24961 aattagaata atagtttgaa aaattaagcg atatagggat tgctcatatt ttatcaatgt
    25021 attctataca aaattatttt aggtcccata ttccgaattt aaagagcgtt tgaaatgggt
    25081 ctaaaaatgt ttgaaactaa ttgaatgtgt gtataatagt aaaaatgcag taaatatccc
    25141 gacaatttct attgacaatt caaacagatt ggtttataat taatataaca acaaatgaaa
    25201 gcgcaaactt tcgcggtcgg aaggtagttt tatgacacga tttgagatac gagatgattt
    25261 ctatctcgat ggaaaatcat ttaagatttt atctggtgcc attcattatt ttagggttcc
    25321 tccagaggat tggtatcatt cgctctataa cttgaaggct cttggtttta atacggtaga
    25381 gacttatgtt gcttggaatt tacacgagcc tcgtgaaggt gagtttcatt ttgaaggtga
    25441 tctggattta gagaaatttc tccaaatagc gcaggatttg ggtctctacg caattgtgcg
    25501 tccgtctcca tttatctgtg cggaatggga attcggtggc ttaccagctt ggctcttgac
    25561 caagaacatg cgaattcgct catccgaccc agtatatatc gaggcagttg gtcgctacta
    25621 tgatcagtta ttgccaagac tggtgcctcg tttgttggac aatggtggca atattctcat
    25681 gatgcaggtt gaaaatgagt atggttctta cggagaagat aaggcttacc tgagagcgat
    25741 tcgacagcta atggaagagt gtggcgtaac ctgtcccctc tttacatcag atggtccatg
    25801 gcgagctact ctgaaagctg gaaccttaat tgaagaggac ctctttgtaa caggaaactt
    25861 tggttctaag gcaccttaca acttttcgca gatgcaggaa ttctttgatg aacatggtaa
    25921 gaaatggcca ctcatgtgta tggagttctg ggatggttgg ttcaatcgct ggaaagaacc
    25981 gattatcaca cgggatccta aggaattggc agatgcagtt cgagaggttt tggaacaagg
    26041 ctctatcaat ctttacatgt tccacggtgg tacaaacttt ggtttcatga atggttgctc
    26101 agctcgagga actttggacc tgccacaagt tacgtcttat gattacgatg cccttctgga
    26161 tgaagaagga aatccaactg ctaaatatct tgcagtcaag aagatgatgg caacacattt
    26221 ttcagagtat ccgcagttgg aaccactcta caaagagagt atggagttgg atgctattcc
    26281 actagttgaa aaagtttctt tgtttgaaac cttagatagc ttgtcaagtc ctgtagaaag
    26341 tctctatcct caaaagatgg aggagctggg acaaagttat ggctacctac tttatcgaac
    26401 agaaacaaac tgggatgcag aagaagaaag acttcgtatc attgatggtc gagatagggc
    26461 ccagctgtat gtcgatggtc agtgggttaa aactcaatat cagacagaga ttggggaaga
    26521 tattttttat caaggtaaaa agaaagggct atctaggtta gatatcttga tagaaaatat
    26581 ggggcgtgtc aactatgggc ataagttctt agcggatacg caacgtaagg gaattcggac
    26641 aggggtctgt aaggatctgc atttcttact aaactggaaa cactatccac tcccactaga
    26701 caatcctgag aaaattgatt tttcaaaagg atggactcaa ggacaaccag ccttttacgc
    26761 ttatgacttt acagtcgaag agccaaaaga tacttaccta gacttgtctg agtttggtaa
    26821 gggggttgcc tttgtcaatg ggcagaatct aggacgtttt tggaacgttg gcccaactct
    26881 ctcactttat atccctcata gctatctcaa ggaaggtgcc aaccgcatca ttatctttga
    26941 aacagaaggt caatataaag aagagattca tttaactcgt aaacctacac taaaacatat
    27001 aaagggggaa aacttatgac aattgtagga tgccgtattg atggacgttt gatccacgga
    27061 caagtagcca atctttgggc tggaaaacta aatgtttcac gcattatggt tgtagacgac
    27121 gaagttgtca acaacgatat tgaaaagagt ggtttgaaac ttgcgacacc accaggtgtg
    27181 aaattgagta ttttgccagt tgagaaagct gcagccaata ttcttgctgg taaatacgat
    27241 agccaacgtc tctttatcgt ggctcgtaaa ccagaccgct tccttggttt ggtagaagca
    27301 ggtgtaccac ttgaaaccct taatgttggg aatatgtctc aaacaccaga aacacgttct
    27361 attacacgtt ctatcaacgt agtagacaag gatgtggaag acttccacaa actggcagaa
    27421 aaaggtgtta aacttactgc tcagatggtt ccaaatgatc caatttcaga ctttttgagc
    27481 ttattaaaat aggaaaaaaa tttttaggag gtcattgtta tgatacaatg gtggcaaatt
    27541 ttacttctca ctttgtactc agcttatcaa atctgtgatg agttgacgat cgtttcatct
    27601 gcaggttctc ctgtatttgc tggtttcatt actggtttaa tcatgggaga tgtgactact
    27661 ggtttactta tcggtggtaa cttgcaactg ttcgttcttg gggttggtac cttcggtggt
    27721 gcttctcgta tcgacgcaac ttctggtgcg gttcttgcga cagccttctc tgtttcacaa
    27781 ggaattgatg caccgcttgc cattactaca atcgctgtac cagtagcagc tctcttgact
    27841 tacttcgacg ttcttggtcg tatgactact accttcttcg ctcaccgtgt ggatgctgca
    27901 atcgaacgct ttgactataa aggtattgaa cgcaactact tgcttggtgc gattccgtgg
    27961 gctctatctc gtgcccttcc agtcttcttt gcccttgctt ttggtggtgc ctttgtacaa
    28021 tcagtagtag acttcgttga agcctacaaa tgggttgcag atggcttgac actcgcagga
    28081 cgtatgcttc caggtcttgg atttgcaatc ttgcttcgtt accttccagt taaacgtaac
    28141 cttcactacc ttgctatggg atttggtttg acagctatgt tgactgttct ttactcatat
    28201 gtaacaggtc ttggtggcgc tgttgctggt atcgtaggta ctcttcctgc tgaagttgct
    28261 gaaaaaattg gtttcgtgaa caacttcaaa ggtttgtcta tgattggtat ttctatcgta
    28321 ggtattttcc ttgcagtgct tcacttcaaa aatagccaaa aagtagctgt agcagcacct
    28381 tctacaccat cagaaagtgg ggaaatcgaa gatgacgaat tctaattaca aacttacaaa
    28441 agaagatttt aatcaaatca acaaacgtag cttgtttact ttccaattag gttggaacta
    28501 cgaacgtatg caagcttctg gttaccttta catgatcttg cctcagttgc gtaaaatgta
    28561 tggtgatgga actcctgaat tgaaagaaat gatgaaagtt catactcaat tcttcaatac
    28621 ttcaccattc ttccatacca ttatcgctgg ttttgacctt gccatggaag aaaaagatgg
    28681 tgtaggttca aaagacgctg ttaacggtat caagacaggt ttgatgggac cattcgctcc
    28741 tcttggggat acaatctttg gttcacttgt acctgctatc atggggtcag tcgcagcaac
    28801 tatggctatc gctggccaac cttgggggat cttcctttgg attgcagttg cagtagcgta
    28861 tgacatcttc cgttggaaac agttggaatt tgcttacaaa gaaggggtta accttatcaa
    28921 caacatgcaa agtaccttga cagctttgat tgacgctgca tctgtacttg gtgtcttcat
    28981 gatgggtgct cttgtagcaa cagtgattaa ctttgaaatt tcttacaagt tgccaatcgg
    29041 tgaaaagatg attgatttcc aagacatctt gaaccaaatc ttcccacgtt tgcttccagc
    29101 aatctttact gcctttatct tctggttgct tggtaaaaaa ggtatgaact ctactaaagc
    29161 tatcggtatt attatcgtac ttgctttggc tctttctgcc cttggtcact ttgcacttgg
    29221 aatgtaattc cttatgacta aatcattaat tttggtgagc catggtcgct tctgtgagga
    29281 gcttagaggt agcacagaaa tgattatggg cccacaagac aacatttaca cagtagctct
    29341 tcttccagaa gatggcccag aagaatttac tgctaaattt gaagctgtta ttgaaggatt
    29401 ggatgatttc ctagtctttg cggatcttct cggtgggaca ccttgtaatg tggtgagtcg
    29461 cttgatcatg gaaggtcgtg atattgacct ttacgcaggg atgaatcttc caatggtgat
    29521 tgaatttatc aatgcgagcc ttacaggcgc agatgcggac tacaagagcc gtgctgcaga
    29581 aagcattgtg aaagttaatg acctgttagc gggcttcgat gatgacgaag atgaataata
    29641 ctcttcgaaa atctcttcaa actacgtcaa cgtcgccttg ccgtaggtat atgttactga
    29701 cttcgtcagt cttatccggc aacctcaaaa cggtgttttg agctgacttc gtcagtctta
    29761 tccggcaacc tcaaagcagt gctttgagca gcctgcggct agtttcctac agattttagt
    29821 tggaactcga ttcaattcat gtgacaacgt gaaaatcgtt agagcatttt atatagaata
    29881 tacatgggaa tggagcttac tcccattccc atatttaata gaaaaagagg aactcaatgc
    29941 tacattatac aaaagaagac ttgctcgaat tgggtgcaga aatcactacg cgtgaaatct
    30001 accaacagcc tgatgtatgg agagaagctt ttgaatttta tcaagcaaaa cgtgaagaaa
    30061 ttgcagcctt tctacaagaa atcgctgata aacatgacta tattaaggtt atcttgacag
    30121 gtgctgggac ttctgcttat gtgggagata ccttgctacc ttactttaag gaagtctatg
    30181 acgaacgcaa atggaatttc aatgctattg cgacaacaga tatcgttgcc aaaccagcaa
    30241 cctatttgaa aaaagatgtg gcaactgtcc ttgtgtcttt tgctcgtagt gggaactcgc
    30301 ctgaaagtgt ggcgactgtt gatttggcca aatccttggt ggatgagctt tatcaagtga
    30361 cgattacttg tgcagcagat ggtaaattgg ctcttcaagc tcacggtgat gaccgtaatc
    30421 tcttgctctt gcaaccagct gtctctaatg atgctggatt tgccatgact tctagcttta
    30481 cgtctatgat gttgacaact ctcttggtct ttgatcctac agaatttgct gttaagtctg
    30541 aacgttttga agttgtatct agtcttgccc gtaaagtttt agacaaggca gaagatgtca
    30601 aagagctcgt tgatttagac tttaaccgtg tcatctatct aggcgctggt cctttctttg
    30661 gacttgctca tgaagctcag ctcaagattt tggaattaac tgctggtcaa gttgcgacca
    30721 tgtatgaaag cccagttggc ttccgtcacg gtccaaaatc tcttatcaac gacaatacag
    30781 ttgttttggt ctttggtaca acgacggact acactcgtaa gtacgacttg gacttggttc
    30841 gtgaagttgc tggtgaccag attgctcgtc gtgttgtgct tttgagtgat caagcttttg
    30901 gtcttgaaaa tgtcaaagaa gtggtccttg gttgtggcgg tgtcttgaat gatatttacc
    30961 gtgtcttccc ttacatcgtt tatgcccaac tctttgccct attgacttca ctcaaggtag
    31021 aaaataaacc agatacaccg tctcctacag gtacagtaaa ccgtgtagta caaggtgtca
    31081 taattcacga atatcaaaag taagacagtg tttatgaatt cttgacaaga ggatttgtaa
    31141 attatcagat aaaccataga ttgtcagtac gctttctatg gtttgtttgc ttgagagaaa
    31201 tagtaaaagg agaacagaat gaaagcatac acagagcgtg tatttggaaa tgttgagggt
    31261 gaggatgtct tggcctatcg atttgagaca gacggtggct accaacttga ggttatgact
    31321 tatggtgcga ctatcttgcg ctatgtcgca cctgacaagg ctggaaattt tgccaatgtt
    31381 atcttgggat ttgatgactt tgatagttat gtaggcaata gtcccaagca tggagcaagt
    31441 gtaggtcctg tagcgggtcg tattgcaggt gcgacctttg agctcaatgg taagacctat
    31501 gaccttgagg ttaataatgc tagcaactgt aatcacagtg gttcaactgg ttgggattcc
    31561 agcttgtttg aagttgaaga agtaagcgat catggcttga ctctctacac agagcgtaca
    31621 gatgggacag gagggttccc tggaaatctc aagatttgga tcagttatca cttggaagaa
    31681 actggtgcct atgaaatcag ctacaaggta acgaccgatc aggatacgct ggtcaatcca
    31741 accaaccaca gctatttcaa cttgtctggt gatttcacgc agacgattga ccgtcatgtc
    31801 ttccaactaa acacagaggg catttactca atcgctcctg acggtgttcc tgccaaaact
    31861 ccagaagcca accgtgatgt ggtcaaacac atctacaatg gtgccttgtt gaaggatatc
    31921 tttgcagaag aagatgagca aatccagctg gcatcaggtt tggatcatcc atttgccctt
    31981 cctgcaggcc atgacaatgc tggattcctt tatgaccaaa attcaggtcg cttcctgctt
    32041 ttcaagacag aagctccttg ctttgtggtc tacacagcaa actttgtgga tgaaagtgtc
    32101 atcataggag gtcagccaat gctacagcac aatgggattg ctcttgaagc gcaagcttta
    32161 ccagatgcca ttcacagtga ccttaaaggc caagtcattc ttaaagctgg tcaaaccttc
    32221 accagtaaga cacgttatga acttgttgtg aaataaaaaa agctggtcaa aaagtcttta
    32281 aaattaaaat aacgcatatt gtcaggtgat gaaaagtttg ataatatgcg ttttattatg
    32341 aaatgattta ctttatatta tcctgaaatg gtgttatttt tgagttatct ggcttgactt
    32401 tcttgatgga aattatataa tagttgtaac aatgataatt acaactagaa aagaggacca
    32461 tttatgacct atgaatacaa gagtcacatt tatttggcag agacagcttt aaatgtaaag
    32521 gatttggcca gtcaaacagc cttttatcag caagttattg gtttagaaat cttatctcaa
    32581 actgagacag agtccattct aggccttggt ggaaaagtct tggtacagct gattcaagca
    32641 caagagagcg gagaagtgag ggaatattat ggtctttacc atttggccat tctcttgccc
    32701 acacgcaagg ctttagctga tgtcttgaaa cacctgacag atttacaaat tcctcttgtc
    32761 ggcggtgcag accatggtta cagtgaagct atttacttgg aagatttgga gggaaatggc
    32821 attgaactct atcgagataa gccagtttcc acatgggata ttcgagaaga tggacgcatt
    32881 atcggagtga cagaggctct tgcggcgcag gacatttatg agttggggga aagagtagag
    32941 ccctttatcc tggcagaagg tacgagaatg gggcatattc atctttccgt caaggatagt
    33001 cgaaagtcca gccagtttta tcaaaaggtg ttagggctag aggataaatt cagtgtgcct
    33061 agcgctagtt ggattgcggc tggagattac catcatcatt tagcagttaa cgaatgggga
    33121 ggaaaaggtc tggcttcgcg taagcaaggc ttaccaggtt tagcctacta tgtcatcgaa
    33181 gtcgcacaca aaaaagaact attaacgatt gcccaacgag cacaagaagt tgacgcacca
    33241 atcaaatgga tgacatcgag ccaattggaa atcacagact cagatggcat cgtgacccgt
    33301 attcgtttag caaggtaaaa ttaggaattt ttaataagtt ataagtattg ctagtttgct
    33361 ttttgaacgg tctttggtaa cagaactgcg tgagctggat gggcgagaat tttgacaaag
    33421 aagctgatta gagatataat gaagaaaaat cgagcaagga aaaagaaatg acaaattcaa
    33481 agtatattac ttgtttgaaa cgttcagagg gccagttgtg tgggattcaa aagatgattg
    33541 aaggagatcg tgactgtgct gatattgtga cgcaactgac agcagtgaga tctagtgtag
    33601 agcgcgtgat tgagatgata attaccgaaa atcttactga atgtatcaat cagccactag
    33661 atgattctga agctcaaaag gaacgcttag aaaaggctat ccgatacttg attaaacgga
    33721 aataaatgaa tgttttgaga cgatatgtgc aaaggtctat taactattag aatagtttaa
    33781 tgggagtcaa aagaagttgt aaaaaatctt taaaattaaa aaaacgcata gtatcaggta
    33841 ttgaaatgta ctgaccccaa aaagttagat tttttctgtc taacttttgg ggtgcagttc
    33901 ataagaacct tggtaatatg cgttttatta tggggagatt tatacttttc gaaaatctct
    33961 tcaaaccagg tcagcttcac cttgccgtag atatggttac tgacttcgtc agttctatcc
    34021 acaacctcaa aacagtgttt tgagctgact tcgtcagttc catccacaac cttaaaacag
    34081 tgttttgagc tgacttcgtc agttccatcc acaaccttaa aacagtgttt tgagcagcct
    34141 gcggctaact tcctagtttg cactttaatt ttcattgagt attacttcat cttctcatga
    34201 aattgagttt ttgcccagtt cctcttgatt attctacttg accaggccaa gcattcatac
    34261 caccttctac gttggtaacg gtgaggcctt gggagatgag aaattggcag gtagatgcag
    34321 agcggactcc tccttgacaa ataacatagt attcatggtc aggtttgagt tctttgtagc
    34381 cttgctctaa ggtacttaat ggaagatttt tggcacctgg tgcatgtcct gcttggaatt
    34441 catgtgcttc acggacatcg ataagttcta gattttcatt ttggtatttt ccataaaagt
    34501 cagccatgct gatactagtt tccatattct actccttctg ggttaaccat tttgtatagt
    34561 gaataagcgc cgtcaaggtt ttgggcggta aatcctgctt gcttgaggat aggctctgcg
    34621 atatagctgc gcaaaccact gtggcagctg acgatgtagg cttgtttttt gtccagttca
    34681 tcagcttcct ccttgacact ttgtcagttt ttgatacaat agtacaaaat tagaggaggt
    34741 gggctatgat tcagaaacat gcgattccta ttttagagtt tgatgacaat cctcaggcgg
    34801 ttatcatgcc caatcacgag gggctggact tgcagttgcc aaagaagtgt gtttatgcat
    34861 ttttagatga ggagattgac cgctatgcaa gggaagtagg ggcggattgt gtcggcgaat
    34921 tcgtttctgc taccaagacc tatccagtct atgtcatcaa ctacaagggt gaggaggtct
    34981 gtctggctca ggctcctgtt ggctccgctc cagcagccca gtttatggat tggttgattg
    35041 gctatggtgt ggagcagatt atctctactg ggacctgtgg tgttctagct gatatagagg
    35101 aaaatgcctt tctagtccct gttcgcgctc tgcgagatga aggagccagt taccactatg
    35161 tggcaccttc tcgttatatg gaaatgcagc cagaggctat tgctgctatt gaggaagttt
    35221 tggaagacag agggattcct tatgaagaag tcatgacctg gacgacagac ggtttttacc
    35281 gagaaacggc tgaaaaggtg gcttatcgta aggaagaagg ctgtgctgtt gtggagatgg
    35341 agtgttctgc tcttgcggca gtagctcaat tgcgtggggt tctctggggt gaattgttgt
    35401 tcacagcaga ttctctagcg gacttggacc agtacgacag tcgtgactgg ggctcggaag
    35461 cttttaataa ggcgctagaa ctgagtttag caagtgttca ccacctttag ttgtactggc
    35521 aaaggatttg ttttatcata aaatgtctag ctcatacttt tcaaaaatat gtttaaacga
    35581 ggtcaccttc ctcttgtcct aagcatgttg aggttgggaa aaatctttaa aatcagaaaa
    35641 acgtatcata tcaggtgatg aaaaccttga tactatgcgt tttatgttgg taagatttag
    35701 agtgagatga aatgatactc ttcgaaaatc ttttcaaacc aggtcagctt caccttgccg
    35761 taggtatatg ttactgactt cgtcagtctt atccggcaac ctcaaaacgg tgttttgagc
    35821 tgacttcgtc agttctattt gcaacctcaa aacagtgttt tgagcaatct gtgactagct
    35881 ttctaatcga tgccttggtt ctcattgcct ataatcaaaa agagaaattt tctcctgaaa
    35941 agcatataga gtagctggcg ttaaaagctc ctgtcttgct tttttgacct atagtcacat
    36001 ctatcaagta ttgttcttgc ctaagctatc aataaaaagg tggcattttt taggcttggt
    36061 gttagtagat tttgccttat cctatctaag tcatttcgaa ctttttatgg tacaatggaa
    36121 acatgttatt caaattatct aaggaaaaaa tagagctagg cttatctcgt ttatcgccag
    36181 cccgtcgtat ttttttgagt tttgccttgg tcattttact aggctctctt cttttgagct
    36241 tgccctttgt ccaagttgaa agctcacgag cgacttattt tgatcatctt ttcactgctg
    36301 tctctgcagt ctgtgtgaca ggtctctcaa cccttccagt agctcacacc tataatatat
    36361 ggggccaaat aatctgtttg ctcttgattc agatcggtgg tctagggctc atgaccttta
    36421 ttggggtttt ctatatccag agcaagcaaa agcttagtct tcgtagccgt gcaactattc
    36481 aggatagttt tagttatgga gaaactcgat ctttgagaaa gtttgtctat tctatttttc
    36541 tcacgacctt tttggttgag agcttgggag ctattttgct tagttttcgc cttattcctc
    36601 aacttggctg gggacgtggt ctttttagtt ccatttttct agcgatctca gccttctgta
    36661 atgccggttt tgataattta gggagcacca gtttatttgc ttttcagacc gatttactgg
    36721 tcaatctggt gattgcaggc ttgattatta caggcggcct tggttttatg gtctggtttg
    36781 atttggctgg tcatgtagga agaaagaaaa aaggacgtct gcactttcat acgaagcttg
    36841 tactattgtt gactataggt ttgttgttat ttggaacggc aactactctc tttcttgagt
    36901 ggaacaatgc tggaacgatt ggcaatctcc ctgttgccga taaggtttta gttagctttt
    36961 ttcaaacagt gacgatgcga acagctggct tttctacgat agattatact caggctcatc
    37021 ctgtgactct tttgatttat atcttacaga tgtttctagg tggggcacct ggaggaacag
    37081 ctgggggact caagattacg acattttttg tcctcttggt ctttgcacga agtgagcttc
    37141 taggcttgcc tcatgccaat gttgcgagac gaacgattgc gccgcgaacg gttcaaaaat
    37201 cctttagtgt ctttattatc tttttgatga gcttcttgat aggattgatt ctgctaggga
    37261 taacagccaa aggcaatcct ccctttatcc acctcatatt tgaaaccatt tcagctctta
    37321 gtacagttgg tgtaacggca aatctgactc ctgaccttgg gaaattggct ctcagtgtta
    37381 tcatgccact tatgtttatg ggacgaattg gtcccttgac cttgtttgtt agcttggcag
    37441 attaccatcc agaaaagaaa gatatgattc actatatgaa agcagatatt agtattggtt
    37501 aagaaaggaa agagcatgtc agatcgtacg attggaattt tgggcttggg aatttttggg
    37561 agcagtgtcc tagctgccct agccaagcag gatatgaata ttatcgctat tgatgaccac
    37621 gcagagcgca tcaatcagtt tgagccagtt ttggcgcgtg gagtgattgg tgacatcaca
    37681 gatgaagaat tattgagatc agcagggatt gatacctgcg ataccgttgt agtcgcgaca
    37741 ggtgaaaatc tggagtcgag tgtgcttgcg gttatgcact gtaagagttt gggggtaccg
    37801 actgttattg ctaaggtcaa aagtcagacc gctaagaaag tgctagaaaa gattggagct
    37861 gactcggtta tctcgccaga gtatgaaatg gggcagtctc tagcacagac cattcttttc
    37921 cataatagtg ttgatgtctt tcagttggat aaaaatgtgt ctatcgtgga gatgaaaatt
    37981 cctcagtctt gggcaggtca aagtctgagt aaattagacc tccgtggcaa atacaatctg
    38041 aatattttgg gtttccgaga gcaggaaaat tccccattgg atgttgaatt tggaccagat
    38101 gacctcttga aagcagatac ctatattttg gcagtcatca acaaccagta tttggatacc
    38161 ctagtagcat tgaattcgta aagagggatg acccctcttt tttgatgccc aagatggcaa
    38221 atagagacag aagccccttg tcttctagta aaagttcttc aaaggctgga ctttatggta
    38281 aaatagaagg aagtgacaag agagagtaat actcaatgaa aatcaaagat caaactagga
    38341 aactagctgc gggctgctca aaacactgtt ttgaggttgt ggatagaact gacgaagtca
    38401 gctcaaaaca ctgttttgag gttgcagata gaactgacga agtcagtaac atctatacgg
    38461 caaggcgacg ttgacgcggt ttgaagagat tttcgaagag tataagaaaa aatcagtccc
    38521 ctaaaggagc agattatgaa gttattgtct atcgccattt ctagctataa tgcagcagcc
    38581 tatcttcatt actgtgtgga gtcgctagtg attggtggtg agcaagttgg gattttgatt
    38641 atcaatgacg ggtctcagga tcagactcag gaaatcgctg agtgtttagc tagcaagtat
    38701 cctaatatcg ttagagccat ctatcaggaa aataaatgcc atggcggtgc ggtcaatcgt
    38761 ggcttggcag aggcttctgg gcgctatttt aaagtagttg acagtgatga ctgggtggat
    38821 cctcgtgcct acttgaaaat tcttgaaacc ttgcaggaac ttgagagcaa gggtcaagag
    38881 gtggatgcct ttgtgaccaa ttttgtctat gaaaaggaag gtcagtctcg taagaagagt
    38941 atgagttacg agtcagtctt gcctgttcgg cagatttttg actaggacca ggtcggaaat
    39001 ttctccaaag gccagtatat catgatacac tcgctgattt atcggacaga tttgttgcgt
    39061 gctagccagt tctaactgcc tgaacatact ttttatgtcg atgatctctt tgtctttacg
    39121 ccccttcagc aggtcaagac catgtactat ctgcctgtcg atttctatcg ttatttgatt
    39181 gggcgtgagg accagtctgt caatgagcaa gtgatgatta agcgcattga ccagcaactc
    39241 aaggtcaatc gactcttggt agaccagctt gatttgtccc aagtgagtca tcctaaaatg
    39301 cgagaatatc tgctgaatca tattgaaatc acgacggtga tttccagtac cctgctcaac
    39361 cgatctgaaa cagcggagca tctggcaaaa aaacgccaat tgtggaccta tattcagcag
    39421 aaaaatccag aagtctttca ggctattcgt aagaccatgt tgagccgttt gaccaaacat
    39481 tctgtcttgc cagatcgcaa actgtccaat gtcgtctatc aaatcaccaa atctgtttat
    39541 ggatttaatt aatataagtg ttttataaga gggatttaag aaaaatttta actttttctt
    39601 agtccttttt aatttcagga gattatacta gagtcatcaa ataaagaaag actctaagga
    39661 gaatcctatg aaattcaatc caaatcaaag atatactcgt tggtctattc gccgtctcag
    39721 tgtcggtgtt gcctcagttg ttgtggctag tggcttcttt gtcctagttg gtcagccaag
    39781 ttctgtacgt gccgatgtgg tcaatccgac cccaggtcaa gtcttacctg aagagacatc
    39841 gggaacgaaa gagggtgact tatcagaaaa accaggagac accgttctca ctcaagcgaa
    39901 acctgagggc gttactggaa atacgaattc acttccgaca cctacagaaa gaactgaagt
    39961 gagcgaggaa acaaactctt ctagtctgga tacacttttt gaaaaagatg aagaagctca
    40021 aaaaaatcca gagctaacag atgtcttaaa agaaactgta gatacagctg atgtggatgg
    40081 gacacaagca agtccagcag aaactactcc tgaacaagta aaaggtggag tgaaagaaaa
    40141 tacaaaagac agcatcgatg ttcctgctgc ttatcttgaa aaagctgaag ggaaaggtcc
    40201 tttcactgcc ggtgtaaacc aagtaattcc ttatgaacta ttcgctggtg atggtatgtt
    40261 aactcgtcta ttactaaaag cttcggataa tgctccttgg tctgacaatg gtactgctaa
    40321 aaatcctgct ttacctcctc ttgaaggatt aacaaaaggg aaatacttct atgaagtaga
    40381 cttaaatggc aatactgttg gtaaacaagg tcaagcttta attgatcaac ttcgcgctaa
    40441 tggtactcaa acttataaag ctactgttaa agtttacgga aataaagacg gtaaagctga
    40501 cttgactaat ctagttgcta ctaaaaatgt agacatcaac atcaatggat tagttgctaa
    40561 agaaactgtc gaaaaagctg tta
//
LOCUS       DAVBBS010000012        39694 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVBBS010000012 DAVBBS010000000
VERSION     DAVBBS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39694
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.012"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..155
                     /locus_tag="VQU32_000618"
     CDS             <1..155
                     /locus_tag="VQU32_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choline-binding protein D"
                     /protein_id="HET2472116.1"
                     /translation="TWYYLEASGAMKASQWFKVSDKWYYVNGLGALAVNTTVDGYKVN
                     ANGEWV"
     gene            556..1677
                     /gene="mnmA"
                     /locus_tag="VQU32_000619"
     CDS             556..1677
                     /gene="mnmA"
                     /locus_tag="VQU32_000619"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET2472117.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            1818..2273
                     /locus_tag="VQU32_000620"
     CDS             1818..2273
                     /locus_tag="VQU32_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2472118.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELDVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLQNIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            2283..4196
                     /gene="mnmG"
                     /locus_tag="VQU32_000621"
     CDS             2283..4196
                     /gene="mnmG"
                     /locus_tag="VQU32_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET2472119.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            4385..4474
                     /locus_tag="VQU32_000622"
                     /pseudo
     CDS             4385..4474
                     /locus_tag="VQU32_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4620..6299)
                     /locus_tag="VQU32_000623"
     CDS             complement(4620..6299)
                     /locus_tag="VQU32_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET2472120.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(6301..6534)
                     /locus_tag="VQU32_000624"
     CDS             complement(6301..6534)
                     /locus_tag="VQU32_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HET2472121.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6916..7137
                     /locus_tag="VQU32_000625"
     CDS             6916..7137
                     /locus_tag="VQU32_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472122.1"
                     /translation="MTNQVLEENYLCDSLLAFSPPRDLCRLVCILLWIKCLDALLMLG
                     FFVWGCRDSRFTTRKFHPIYQDEVVFAFF"
     gene            complement(7350..7502)
                     /gene="cibB"
                     /locus_tag="VQU32_000626"
     CDS             complement(7350..7502)
                     /gene="cibB"
                     /locus_tag="VQU32_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HET2472123.1"
                     /translation="MMKDLNNCREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(7504..7689)
                     /gene="cibA"
                     /locus_tag="VQU32_000627"
     CDS             complement(7504..7689)
                     /gene="cibA"
                     /locus_tag="VQU32_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HET2472124.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            7867..8550
                     /gene="tsaB"
                     /locus_tag="VQU32_000628"
     CDS             7867..8550
                     /gene="tsaB"
                     /locus_tag="VQU32_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET2472125.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMESLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLTHTLNIELVGMSSLLALVPY
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMPEAHLSFEEVLEKVKGASQVTFVGEVGPF
                     VEQIQEHLPRTNYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            8547..8984
                     /gene="rimI"
                     /locus_tag="VQU32_000629"
     CDS             8547..8984
                     /gene="rimI"
                     /locus_tag="VQU32_000629"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET2472126.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            8974..9984
                     /gene="tsaD"
                     /locus_tag="VQU32_000630"
     CDS             8974..9984
                     /gene="tsaD"
                     /locus_tag="VQU32_000630"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET2472127.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(10030..11330)
                     /locus_tag="VQU32_000631"
                     /pseudo
     CDS             complement(10030..11330)
                     /locus_tag="VQU32_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(11606..11957)
                     /locus_tag="VQU32_000632"
                     /pseudo
     CDS             complement(11606..11957)
                     /locus_tag="VQU32_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12064..12356)
                     /locus_tag="VQU32_000633"
                     /pseudo
     CDS             complement(12064..12356)
                     /locus_tag="VQU32_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            12599..13805
                     /locus_tag="VQU32_000634"
                     /pseudo
     CDS             12599..13805
                     /locus_tag="VQU32_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            13798..14446
                     /locus_tag="VQU32_000635"
                     /pseudo
     CDS             13798..14446
                     /locus_tag="VQU32_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            14456..16072
                     /locus_tag="VQU32_000636"
     CDS             14456..16072
                     /locus_tag="VQU32_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2472128.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLSGFLRDYSGELRFCGDDIK
                     KTSYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNI
                     LDYYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDRE
                     DLTVIIVTHAPHPELKQYFTKIYQFPKDFI"
     gene            16092..16936
                     /locus_tag="VQU32_000637"
                     /pseudo
     CDS             16092..16936
                     /locus_tag="VQU32_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813546.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            17004..18505
                     /locus_tag="VQU32_000638"
                     /pseudo
     CDS             17004..18505
                     /locus_tag="VQU32_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819023.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase"
     gene            18517..19266
                     /locus_tag="VQU32_000639"
     CDS             18517..19266
                     /locus_tag="VQU32_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET2472129.1"
                     /translation="MSDNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSKLKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
     gene            19279..20451
                     /locus_tag="VQU32_000640"
     CDS             19279..20451
                     /locus_tag="VQU32_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET2472130.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGTLP
                     SKNEELMKFFCENNLNITFFDTFSEIKNNIDYYIIALPTDYDEKIGSFNTYEIEQTVS
                     KILRVKPNGKIILKSTVPIGFSNKLKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVV
                     GDETVEGRKIAELFLSISTHSTANIKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELD
                     SFAERRSLNAEVVIKGVCLDPRIGNFYNNPSFGFGGYCLPKDTRQLKKEFIEINAPVI
                     EAIDISNTNRKQFIVKQILERKPKIVGIYKLGMKYNSDNYKESAILSIINELLIVGIK
                     ILVYEPNLNVSIDNVIFEKNFELFIKQSDLIVANRWDRGLEAYKDKVYTRGIWIRD"
     gene            20846..21709
                     /locus_tag="VQU32_000641"
     CDS             20846..21709
                     /locus_tag="VQU32_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET2472131.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACAGLTASQLSKFELGQSM
                     LSADKLILAIQGINMNFDEFGHKLNNYQESPHMRIGRRVVDCFSHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKNALHSLDKSYPLTEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRWYMKSGYLNIISELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVLWQFLKKMQIFLQTKGSNQKEIEHFIQSLQVLENPQLTALFE
                     LRLQQYKELID"
     gene            21995..22228
                     /locus_tag="VQU32_000642"
     CDS             21995..22228
                     /locus_tag="VQU32_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472132.1"
                     /translation="MLNLQFAETMELTEAELQDVRGGNLVNSMGGGGRSGISGWGVPG
                     IYPGWGNQGMSPNRGAFDWTIDLADGLFGRRRR"
     gene            22250..22927
                     /locus_tag="VQU32_000643"
     CDS             22250..22927
                     /locus_tag="VQU32_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2472133.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMVALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATSLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            22939..23604
                     /locus_tag="VQU32_000644"
     CDS             22939..23604
                     /locus_tag="VQU32_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2472134.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFFVYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            23676..24902
                     /locus_tag="VQU32_000645"
     CDS             23676..24902
                     /locus_tag="VQU32_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2472135.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLATLEL
                     SALILGSLMSGYLQHTISIKIALYASLLLQLLLLVGFATAHFGLILIFSFLDAFFAGI
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALGTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(25253..25330)
                     /locus_tag="VQU32_000646"
                     /pseudo
     CDS             complement(25253..25330)
                     /locus_tag="VQU32_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            25401..26057
                     /locus_tag="VQU32_000647"
     CDS             25401..26057
                     /locus_tag="VQU32_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HET2472136.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            26047..26370
                     /locus_tag="VQU32_000648"
     CDS             26047..26370
                     /locus_tag="VQU32_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HET2472137.1"
                     /translation="MVSKYLLLAVIFSDLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAV
                     LRLVF"
     gene            26474..27304
                     /locus_tag="VQU32_000649"
     CDS             26474..27304
                     /locus_tag="VQU32_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2472138.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NSSPKPFNYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYQMAV
                     NNISYTKERAEKYLYAAPTVKDPNVLVVKKDDSSIKSLDDVGGKSTEVVQGTTSAKQL
                     EDYNKQHSDNPTILNYTKANLQQIMGHLSDGQFDYKISDKITVETVIKNQGLDNLKVI
                     DLPSDQQPYVYPLLAKGQDELKTFVDKRIQELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            27345..27422
                     /locus_tag="VQU32_000650"
                     /pseudo
     CDS             27345..27422
                     /locus_tag="VQU32_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC protein"
     gene            27526..28356
                     /locus_tag="VQU32_000651"
     CDS             27526..28356
                     /locus_tag="VQU32_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2472139.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NGSPKPFIYEENGELTGYEIEVVRAIFKDSDKYNVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            28510..29364
                     /locus_tag="VQU32_000652"
     CDS             28510..29364
                     /locus_tag="VQU32_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HET2472140.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            29464..30837
                     /locus_tag="VQU32_000653"
     CDS             29464..30837
                     /locus_tag="VQU32_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET2472141.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            30830..31891
                     /locus_tag="VQU32_000654"
     CDS             30830..31891
                     /locus_tag="VQU32_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HET2472142.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            31893..32585
                     /locus_tag="VQU32_000655"
     CDS             31893..32585
                     /locus_tag="VQU32_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2472143.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(32615..33184)
                     /locus_tag="VQU32_000656"
     CDS             complement(32615..33184)
                     /locus_tag="VQU32_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET2472144.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            33319..33933
                     /locus_tag="VQU32_000657"
     CDS             33319..33933
                     /locus_tag="VQU32_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET2472145.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            33935..35581
                     /locus_tag="VQU32_000658"
     CDS             33935..35581
                     /locus_tag="VQU32_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2472146.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDR
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQKYFEHRASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            35593..36879
                     /locus_tag="VQU32_000659"
     CDS             35593..36879
                     /locus_tag="VQU32_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2472147.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGCD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            36940..37473
                     /locus_tag="VQU32_000660"
     CDS             36940..37473
                     /locus_tag="VQU32_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2472148.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRTAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKASAEEEETEELA"
     gene            37551..38021
                     /gene="nrdI"
                     /locus_tag="VQU32_000661"
     CDS             37551..38021
                     /gene="nrdI"
                     /locus_tag="VQU32_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HET2472149.1"
                     /translation="MKIISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(38314..39576)
                     /locus_tag="VQU32_000662"
     CDS             complement(38314..39576)
                     /locus_tag="VQU32_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HET2472150.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFSGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT        12055 a         6876 c         8561 g        12202 t
ORIGIN      
        1 atacctggta ctatcttgaa gcatcaggtg ctatgaaagc aagccaatgg ttcaaagtat
       61 cagataaatg gtactatgtc aatggtttag gtgcccttgc agtcaacaca actgtagatg
      121 gctataaagt caatgccaat ggtgaatggg tttaagccga ttaaattaaa tcatgttaag
      181 aacatttgac attttaattt tgaaacaaag ataaggttcg attgaataga tttatgttcg
      241 tattctttag gtacctatct tatgatttca ggaaatgtca ttaaaaaaac gactcctttt
      301 ctctaacttg aaaaatagat tagagaaaat gggttgtttt atttattata gttatttgaa
      361 tgaagataag aagaaggtat actcacatca ttcacataat ctgtatgttg actataagtt
      421 taaaaaaaca atttttaagc tcttccttgt cttctccaac caagcgtgtt ataatgaata
      481 ctgctcaagc gaccttcaat cgtgaagcac acacgacctt caatcgtgaa taaacgaata
      541 gatgggagac ttaccatgag tgataactct aaaacacgtg ttgtcgtggg gatgagtggt
      601 ggtgttgatt cgtcggtgac ggctcttttg ctcaaggagc agggctacga tgtgatcggt
      661 atcttcatga agaactggga tgacacagat gaaaacggcg tctgtacggc gaccgaagat
      721 tacaaggatg tggttgcggt ggcagaccag attggcattc cctactactc tgtcaatttt
      781 gaaaaagagt actgggaccg cgtttttgag tatttcctag cggaataccg tgcagggcgc
      841 acgccaaatc cggacgttat gtgcaacaag gaaatcaagt tcaaggcctt tttggactat
      901 gccatgacct tgggggcaga ctatgtagcg actgggcatt atgctcgagt ggcgcgtgat
      961 gaggacggca ctgttcacat gcttcgtggc gtggacaatg gcaaggacca gacctatttc
     1021 ctcagccaac tttcgcaaga acaactccaa aaaaccatgt tccctctggg acatttggaa
     1081 aagcctgaag tacgcagact agcagaagaa gcaggccttt cgactgctaa gaagaaagac
     1141 tcgacaggga tttgctttat cggagaaaag aactttaaaa actttctcag caactacctg
     1201 ccagctcagc ctggtcgcat gatgactgtg gatggtcgcg atatgggcga gcatgcaggt
     1261 cttatgtact atacaatcgg tcagcgtggc ggactcggta tcggtgggca acacggcggt
     1321 gacaatgccc cttggttcgt tgtcggaaaa gatctaagca agaatattct ctatgtagga
     1381 caaggattct accatgattc gctcatgtca actagcctag aagccagtca agtccacttt
     1441 actcgtgaaa tgccagaaga gtttacgcta gaatgtacgg ctaaattccg ttaccgtcag
     1501 cctgactcta aggtgaccgt ccatgtcaaa ggagacaagg cagaagttat ctttgcggaa
     1561 ccacaacgcg cgattacacc aggacaggca gttgtctttt acgatggcga agagtgtcta
     1621 ggtggcggtt tgattgacaa tgcttaccgc gatggacaag tttgtcagta catttagatt
     1681 gacaaatttt ctcaatttgc tacaataata acagcaatag aaatgatggt caaagctcat
     1741 ggatgttgca ggcttttttg tcctgcactt ctttgtagtt ttgactgttt ttgtgtcgtt
     1801 taagggaaag gacaagaatg actcaacaag actttcggac aaaagtagac aatacggttt
     1861 ttggtgttcg ggctacagcc ttgattgtcc aaaatcacaa gcttctagtt accaaagaca
     1921 agggcaagta ttacactatt ggaggtgcga ttcaagtcaa tgaaagcacg gaagacgcgg
     1981 tagtccgtga agtgaaggaa gaactggatg tcaaagctca agctgggcag ctagcttttg
     2041 tggttgaaaa tcgttttgaa gtggacggcg tttcctatca caatatcgag tttcattatc
     2101 tggtggattt gcttgaagat gccccgttga ccatgcagga agatgagaaa aggcagccct
     2161 gtgagtggat tgacttggat aagctccaga atatccagct agttccagcc tttttaaaaa
     2221 cagccctacc agattgggaa ggccaactaa gacacattca tcttgaggaa taggagagaa
     2281 acatgactta tcattttact gaagaatacg atattattgt aattggtgcg ggacacgctg
     2341 gggttgaggc ttccttggcc gctagccgta tgggctgtaa ggtcctgctt gcgaccatca
     2401 atattgaaat gctggctttc atgccttgta atccctctat cggtggttct gctaagggga
     2461 ttgtcgtacg tgaagtcgat gccctcggtg gcgagatggc caagaccatt gacaagactt
     2521 acatccagat gaagatgctc aacacaggga agggaccagc cgttcgtgcc cttcgtgcgc
     2581 aggctgataa ggaactttac tctaaggaaa tgcgcaagac agttgaaaat caagaaaatc
     2641 tgacccttcg tcaaaccatg attgatgaga ttttggtgga agatggcaag gttgtcggtg
     2701 tgcgtacagc cacccatcaa gaatatgctg ctaaggctgt tattgtgacg acagggactg
     2761 ctctccgtgg ggaaattatc atcggagacc tcaagtactc atcaggtcct aaccacagct
     2821 tggcttctat taacctagct gacaatctca aggaactggg tctcgaaatc ggtcgtttca
     2881 agacaggaac ccctccacgt gtcaaggctt cttctatcaa ttacgatgtg acggaaattc
     2941 agccaggaga cgaagtgcct aatcatttct catacacttc acgtgatgag gattatgtca
     3001 aagatcaagt gccatgctgg ttgacctata ccaatggtac cagtcatgag attatccaaa
     3061 acaacctcca ccgtgcgcct atgtttacag gtgtggtcaa gggagtgggg cctcgttact
     3121 gtccgtcgat tgaagacaag attgtgcgct ttgcggacaa ggaacgtcac caactcttcc
     3181 ttgagccaga agggcgcaat actgaggaag tctatgttca aggactttca accagtctgc
     3241 ctgaggatgt ccagcgtgac ttggttcatt ccatcaaagg tttggaaaat gcagagatga
     3301 tgcggacagg ttatgctatt gagtatgata tggtcttgcc tcatcagttg cgtgcgactt
     3361 tggaaaccaa gaaaatctca ggtctcttca ctgctggtca gacaaatgga acatcaggtt
     3421 atgaagaagc tgctggccaa gggattatcg cgggtatcaa tgcggctctg aaaatccaag
     3481 gtaaacctga gttgattcta aaacgaagtg acggttatat cggggtgatg atcgacgact
     3541 tggtgaccaa gggaaccatt gaaccttacc gtctcttgac cagtcgtgct gaataccgtc
     3601 tcattcttcg tcatgacaat gctgatatgc gcttgactga gatgggacgc gagattggcc
     3661 ttgtggatga tgaacgctgg gctcgttttg aaatcaagaa aaatcaattt gataatgaga
     3721 tgaaacgcct agacagtatc aaactcaagc cagtcaagga aaccaatgct aaggttgagg
     3781 aaatgggctt caagccgttg acagatgcgg tgacagccaa ggaattcctt cgccgtccag
     3841 aagtttctta ccaagatgtg gtggccttca tcggaccagc tgcagaagac ttggatgaca
     3901 agattatcga attgattgaa acagaaatca agtacgaagg ctatatttcc aaagccatgg
     3961 atcaggttgc taagatgaaa cgtatggaag aaaaacgcat tccagccaat atcgactggg
     4021 atgacatcga ttctattgcg acggaagctc gtcagaagtt caaactcatc aatccagaaa
     4081 ccatcggcca agccagccgt atttcgggag taaacccagc agatatttct attttgatgg
     4141 tgtatctgga aggtaaaaat cgtagtattt ctaaaactct tcaaaaatca aaatgatacg
     4201 tcgtcggctt cttacgaatg agttcaaagc ttggctttga ttcatctcca acctcccata
     4261 gttccccgaa ctatgggagc taactcaagt tatgcatttt cctagccttc tggtctgatt
     4321 ttcattgagt tttaatctca aaagaaagca tagagaaaaa cagctccgaa gacggggctg
     4381 ttttgttgac ttgtactcaa tgaaaatcaa agagcaaact aggaagctag ccgtaggttg
     4441 ctcaaagcac tgctttgagg ttgtagatag aactgacgaa gtcagctcaa aacacagttt
     4501 tgaggttgta gatagaactg acgaagtcag ctcaaaacac agttttgagg ttgtggatag
     4561 aactgacgaa gtcagtaaca tatatacggc aaggcgaagc tgacgtggtt tgaagagtat
     4621 tattcttcat ctggtgtgag gatcatcggg atgatgatcg gttcacgttc ggtattttca
     4681 tagaggaagg ggcgaatagc gttgacaatg gcaccattga cagattgcac gctagcatcc
     4741 ttatttttca gtgcgatacg aatggcattg aagaggatac gctggctttg gcgaatcaag
     4801 tctccagact ctctcatgta gacaaagcct cggctgagga tatctgggcc agacagaatc
     4861 atctgcgatt tgaagtcaac agttgcgact gccagaacga caccgtcttc agatagatcg
     4921 cgacgatctt tgaggacagc tgcgccaatt tcaccgatac gatttccatc gacatagata
     4981 tcttgggcgt tgaaatgacc tgcgatacga gctgagtcag cagtaagggc aagcacatcg
     5041 ccattgctca tgataaagat attgtctttc tcaacaccag tatccactgc tagtccagcg
     5101 tggacttttt gcatgcggta ttcaccgtgg acaggcatga agtattttgg cttaatcaag
     5161 cggagcatga gtttttgctc ttgctgacca ccgtgtccag atgtatggat attgttcact
     5221 ttaccgtgga taacttcgac accagcttca gaaatgatgt taatcagctt gttgacgcta
     5281 gtagtgtttc cagggatggg actagaagag aagataacgg tatcacctgg ttgtaattgt
     5341 acttgacggt gggttccgtt ggcgatacga gagagggctg ccataggctc accctgacta
     5401 cctgtacaga ggataagaac ttctcctgca ggataatctt tgatttcatt tggctcgata
     5461 aaggttccct taggagcttt gatgtagcca agatcgattc cgttgacaat ggccttttcc
     5521 atagaacgac caaagaccgc aatcttgcgt ccagtcttaa cagcagcttc tgttgcctgc
     5581 tggagacgga agatatttga ggcaaaggat gcaaagatga tacgtccttc aataccttgg
     5641 ataatcttca taatggactg accaacgact ttttcagagt tggtaaaggt tggtacttcc
     5701 gcatttgtcg agtcagacag gagacagagc acgccttctt caccaagcgc agccatacga
     5761 tgcaagtccg caggttctcc aactggagta aagtcgaact taaagtcacc cgtacagacg
     5821 attttccctt gaggagtatg aatgacaatc cccaaaggct ctggaataga gtgagtcgtt
     5881 ctaaagaaag ttgccttgag atttttaaag gtcaactcgg tgttgtggtt gatttcgtaa
     5941 agtttggcgt tgcgcaagag gccgtgttct tcgagtttcc cacggatcaa agccaaggca
     6001 agcggtccag cgtagatagg gacatttgct tgcttgagta ggaacggaat cccaccaatg
     6061 tggtcctcgt gtccgtgtgt gattaaaaca gccttgacgc ggtcgatatt gtctacgatg
     6121 taagagtagt caggaatgac atagtcgata ccaagcaagt catcttctgg gaatttaatc
     6181 ccagcatcga cgatgataat ctcgtcttgg tattcaattc cgtaagtgtt tttcccgatt
     6241 tctcctagac caccgatggc aaaaacaccg acttcttcag gtttaagagt gtaggccata
     6301 ttagaactcc gtaatttcga aggcgccagt ttctttttcg taatcgagca atttgtcaga
     6361 caagagttcg atatattcga tattgtactc tgggcgattt tcttcgacaa gttggcgagc
     6421 agtgatacgg ccctcaagtt ctgagctggc atcgatgtct aggtaaagcg cgcgtgttgt
     6481 ttcacggcgt gggctacgtt cttttgtttc ttgataaaaa actttgtaaa tcatatagtt
     6541 cctttctatt tacaggtcag cttattccaa acttttagca gatatttgct tgatttcatt
     6601 ccattttgtg tctagattga ccttgattcg ttacaattat ttcaattata ccacaaaatg
     6661 aaaaattagt aaaagacgga aacgagaaag ttaggggacg atttcttcgg taagcgtttg
     6721 caaaaacaaa gaaaatgtgt tatgttgata acggaagtga agtttattac ttgtcataat
     6781 tccatataga ttttaagtca aggaggttgc cctatgtata acttatttgc ttttgctatc
     6841 ggagttcgtt tgctagcttt tatcgttttt gttttgcgtt tatggaggaa atcttctatt
     6901 tgccaaactg gaacgatgac aaaccaagtt cttgaggaaa attacctttg tgactctcta
     6961 ctcgcttttt ctcctcctcg tgacctatgt cgtttggttt gtattttact ttggattaaa
     7021 tgcttagatg ccctgcttat gctggggttt tttgtttggg gatgtagaga tagtagattt
     7081 acgacacgaa aattccaccc catctatcaa gatgaggtgg tctttgcatt tttctaaaat
     7141 gatatgacaa tggaattttc aaagcttatt atttttatgg gagtcattgt tctgctcaaa
     7201 aatagagttg aagcataaaa ataaaacaat acaaacagat aaatcgaatg gtaaagggat
     7261 ggtagtaatg ttcaaatcat tgagaatgct gagcaaactg taagagatga agacaataaa
     7321 atttatcctt ctaaaaatgt gtttcttatc tagcatgact taccaaactt tttacgaagg
     7381 tcttttccaa tggtataacc tgccataaat aaagcgatac caacacctac accaaagcca
     7441 cccttgattt cttgcaattc cttattagaa atttcacgac agttgttcaa atctttcatc
     7501 atattaatct cctcctaaaa aataaccagc tgccaaacca gaacctataa gtgctgttcc
     7561 acctgcaata gctttccaag atactgctac tccaaagata accaagggag cgagaccgcc
     7621 atcaatatct gataattcat tttcagataa ggataaaaat tgattgtcaa gaatgtcaaa
     7681 atttgtcatt gaactttcct ccttttttct tgttcacttt tatattcgga aaagaaaagg
     7741 aaaagtgagc atttttttaa attattttta gttaagtgag ggcactcttg tctgggggca
     7801 aagaaaattc ccatgtgcca tcttttgtag tataatagta agcagacaaa aagataggaa
     7861 tgtgttatga aagtattagc ttttgatacg tccagcaagg ctctttctct cgctatttta
     7921 gaggataagc aggttcttgc cgagacgacg attaatatca agaaaaatca cagtattacc
     7981 ctcatgccag ccattgattt tttgatggag agtttggact ggacgcccaa ggatttggac
     8041 cgaatcgtgg tggcggaagg gccaggtagc tacacaggct tgcgaatcgc ggtagccact
     8101 gccaagacct tgactcatac tctgaacatt gagttagttg gtatgtctag cctcttggct
     8161 ctggtgccat atcaacaaga aggcttgttc gttcctttga tggatgcgcg tcgcaacaat
     8221 gtttatgcag gattttatga aaatgctaaa cctgtcatgc cagaagcgca cctatctttt
     8281 gaagaggtgc tagaaaaagt caagggtgct agtcaggtaa cctttgtcgg agaagttggc
     8341 ccctttgtgg agcagattca agaacacttg ccaaggacta attataaaga aaccttgcct
     8401 aatgcagcca atcttgctct tttagcttgg gacaaggaag cagactcctt gcatgatttt
     8461 gtgccaaatt acctcaaacg agtcgaggct gaggaaaact ggctcaagaa ccataccgag
     8521 tctggtgagt cctacattaa acgcctatga tagaaatcaa gcgaattcaa caacagcctg
     8581 acctagctca agccatctac gctgttatgg cagctgttta cctagtcagt ccttggactc
     8641 tggagcaaat ccaagcagat ctgtcccaag accagacttg gtatgcattg gcttatgatg
     8701 gggcagaagt gattggattt ctagctgtgc aggagaatct ttttgaagca gaagtcctgc
     8761 aaatcgctgt caaaggagct tatcagggtc agggaattgc gtcagccttg tttgctcaat
     8821 tgccgacaga taaggagatt ttcctcgaag tcagaaagtc aaatcaacga gcgcaagcat
     8881 tttacaagaa agaaaaaatg gcggtcatcg ctgagcgaaa ggcctactac catgacccag
     8941 tcgaggacgc cattatcatg aagagagaaa tagatgaagg atagatatat tttagcattt
     9001 gagacatcct gtgatgagac cagtgtcgcc gtcttgaaaa acgacgatga gctcttgtcc
     9061 aatgtcattg ctagtcaaat tgagagtcac aaacgttttg gtggcgtagt gcccgaagta
     9121 gccagtcgtc accatgtcga ggtcattaca gcctgtatcg aggaggcatt ggcagaagca
     9181 gggattaccg aagaggacgt gacagctgtt gcggttacct acggaccagg cttggtcgga
     9241 gccttgctag ttggtttgtc agctgccaag gcctttgctt gggctcacgg acttccactg
     9301 attcctgtta atcacatggc tgggcacctc atggcagctc agagtgtgga gcctttggag
     9361 tttcccttgc tagccctctt ggtcagcggc ggacacacag agttggttta tgtttcggag
     9421 gcaggagatt ataagattgt tggggaaacc cgtgatgatg cggttggtga ggcttatgat
     9481 aaggtcggcc gtgtcatggg cttgacctat cctgcaggtc gtgagattga cgagctggct
     9541 catcaggggc aggatattta tgatttcccc cgtgccatga ttaaggaaga taatctggag
     9601 ttctccttct caggtttgaa atctgccttt atcaatcttt atcacaatgc cgagcaaaag
     9661 ggagaaagcc tgtctacaga agatttgtgt gcttccttcc aagcagcagt tatggacatt
     9721 ctcatggcaa aaaccaagaa ggctttggag aaatatcctg ttaaaaccct ggttgtggca
     9781 ggtggtgtgg cagccaataa aggtctcaga gaacgcctag cagccgaaat cacagatgtc
     9841 aaggttatca tcccccctct gcgactctgc ggagacaatg caggtatgat tgcctatgcc
     9901 agcgtcagcg agtggaacaa agaaaacttc gcaggctggg acctcaatgc caaaccaagt
     9961 cttgcctttg ataccatgga ataaagagtg gctctttgtc aagtgtagtg ggtagtcgaa
    10021 aagctgcaat ctggagagta cgaaattcgt actctctttt ttgatgttca aagtcatcaa
    10081 aatccgtttt ttaaaattgt taaagttccg gaaaccaaag gctttgcgct tgataatctt
    10141 aatcaaatta ttggtcgctt caagtttagc gttggaataa ggcagcttaa gtgcgttgat
    10201 aatcttatct ctatccctta aaaaagtcca aaagaccgtt tgaaaaagcg gatgaaccgt
    10261 tggcagttct tgctcaatca atccaaagaa atggtcggca ttcttctctt gaaaatgaaa
    10321 gagcaggagt tgatagagtt cgtagtgaac ctgtaatccc tcagagtaag aaagcagctt
    10381 atctcgtact tcccgatggg ttaagtgcat tcgaaacgtg ggctgataga atcgtttctg
    10441 acagagcttg aagctatact ggtgtagtaa atcagtccag tggactgatt tagcccgagc
    10501 ctagaaacga aaaagcgagg gttccaaaag cgcttgagcg cccgatactc caaggatttt
    10561 cggtcaaatt ggttcatgat ttggattcgt actcggttca tagctcggct cagatgttgg
    10621 acaatgtgga agcggtcaag aacaatcttc gcctttggaa agagttgctt agcgagccga
    10681 taataagggc tgtacatgtc catggtgatg acctcgacca gctcccgaac ctctctctga
    10741 tagcgttgga aatggtttcg aatcaccgca tgagttcgcc cgtctaaaat tgcgaggatg
    10801 gatttggact caaaatcttg ggcaatgaag ctcattttgc tctttttgaa gctatactca
    10861 tcccaactca taacttttgg caacttggtc caatcggttt caaacttaaa ttccctcagt
    10921 tttcggatga cggtggaagt tgagaccgcc aatctgtggg caatctcagt catagactgc
    10981 ttttcaagga ggagctgagc gattttttgt ctcaccatgt tggaaatctg gcaatttttc
    11041 ttgacaatgg atgtttgaga aacgaccacc ttaaggcaat tcttgcactg aaagcggcgc
    11101 tttttgagat gcagtaccgt gggtaaaccc tgacagtcga gaagcggaat tttagaggct
    11161 ttctggaagt cgtatttgat catcttccct tgacaatgag gacaaggagg agcgggggaa
    11221 tccaactttg cctgaacgac tagatgggtt tggtatttca gaacagacaa gattttgata
    11281 tttttgtctt ccaatccgag caaatctgtg gtattcttaa gttgttccat atggaccctt
    11341 tctaatgagt tggttagacg cttttcatta taggtcatat ggggcttttt ttctacaaga
    11401 aacgacccta taattcctgg ggtgggatta cccactacag aaattataga gccaaagcat
    11461 tccaaagtct tgtctgactc tggaatgttt tctgttcaca gttgttttgt aactgcctaa
    11521 taagtaaccg cgctataacg aggtatctac cgagattgtc aattctccag tatactgtta
    11581 taaaaaacaa aactggagga tttttttatg tcctcccatc gtcccatcga ctgttccgta
    11641 gctgcccaat aacggagtat agtcggctga aacaagaata ggacaaaagt gcctcaagaa
    11701 aagcatcgta atttgacaat attattctga gatgtttttg atgtaagccc atattttctc
    11761 aatgggatta tactcgggtg aataggtagg aagtggtaac agtctatgcc ctgctcctta
    11821 cacatgttca ttctgtgaaa ccttgcattg tccataatga taagggatgg tgtatctaaa
    11881 gtaggtagta agaatttttg gaagcaagct tcgaaaaagt cactcgtcat cgtatcttta
    11941 taggtcatcg gagccatagt atattgaaac aaaaaagggc attagtactt ctataacagt
    12001 atcgcaatct ctggtgtgcg ttgtctcgat cttaattcaa ataattatat cttcttccag
    12061 agagactaac tgcctgctca aggctcgatc atatttgaga taaaaatagg tctcaacccc
    12121 tgtctcataa atataaatag gagtcaggta gcttaagtga ttcaattctt taaggaaccg
    12181 atttactttt tctgggtctt gttcgcagta ggtacggctc tttttagact atatcccata
    12241 gcctttagag cgtaatgaat agttgttgga ggacagttga attcagcagc tatttcagtc
    12301 aaataagcat cgggatgagt ttcaatatag atctttaatt tatctctatc aactttttca
    12361 attaatagtg gaagttttct taaagttagt gtgttgaaac gtttttatac agtgctaaaa
    12421 tttgaagtgt agtgcagatg tatttgtaat agtattatag caattctttg gagaaggtcg
    12481 agacaagaga aaataggtat ctttgagata taagtatgat ttactataaa attattgagt
    12541 tattcataaa aatttctcta tcaaatatca taatgcgtcg tttaaggaga tggttacaat
    12601 gcaatatagt tgtggaaaaa taaatattaa tatccctgat ggatatggag acattaagga
    12661 tatagtgttt tcggctcata ttatagttag gtataataat ggacattgtg gaggtataga
    12721 tccgcatata attggacttt gtaaaaagca gatcagaagg atgtctccat atcctatttt
    12781 gataatcgta tctagagatt caaaggttat tgatgattat aaaaatttag atattgccta
    12841 tgttgattgt actcaatgtt caaataattt tgaaactgcc ttacacgtta aaaatatttt
    12901 aaaattatta aaaatccaac ttatacactg tcatggctac tcaactaatt attttttata
    12961 tatgttaaaa aaacttgaca agaatggatt tggaaaggtt aagacagtga taacatgcca
    13021 tggatgggtt gaatataact taaaaaagaa attcttgact tattttgatt tttggacata
    13081 ttctatggga gatgctttta tttgtgtatc tgaaactatg aaaaaaagat tggagagtat
    13141 aataaaaaaa taagaagata gttgcaataa ataacggaat taatgtatca aatagcgatt
    13201 tagatgtagt aggtgttcaa gactttaaaa aagaattttg tattccaaat aacaaaaaaa
    13261 tcatttgtta tgttggaagg ttggatccag aaaaaaggcc ggatagattc cttgaatttg
    13321 cagaaaaatt atttttagtc agagaagatg tgatttttat tatggctgga aatggtagta
    13381 tgtgggctac tttgaaagaa aaaatttgcc atttgaaatg tagagataat ttcaggttac
    13441 ttggtgaaat ctatccagct actatagttt accaaatatc ggatttattg tatattcctt
    13501 ctgatacaga aggaatacct atgtgtgttt tagagtcaat gtcacaagaa actccggttt
    13561 tagctagtaa tgttggcggg ttaagtgaaa ttattgaaca tagggttgat ggatttttat
    13621 ttgagaagga agatgttgag ggagtgtgtg cttgtgctaa ttttttactc aatgattctg
    13681 agtatttgaa atatataggt gagaatagta aatcaaaaat aagaaaacat ttttctgtgc
    13741 aaaaaatgtt tgtagaaacc atgagagtat atgatgaatt attagagaag agtagtcatg
    13801 gataggaata tagatcaaga attagtaagt attataatac caacacataa taggtatgaa
    13861 tctctgatta gagctgtgaa aagttgttta catcaaagtt ataaaaatat cgaagttata
    13921 attattgatg ataattatag taatgtaaat ttaagaaata aaattatcca tcaatttggc
    13981 tataccagtc atagaattaa gttaatttta agtaatgaag atttaggtgc aactaatgcc
    14041 agaaacatag gtatcaaaaa ttctagaggt aagtatatat catttttaga cgatgatgat
    14101 gaatatatgc cagatcgaat tttaaagttg atggcttgtt ttaaaaagag tagaatgaag
    14161 aatttagctt tagtttatag ttatggaata ataatttatc caataggaaa tattttaatt
    14221 tattgagttt caacgagaga atattggtta caaagaaata ttattcttta aacataaagc
    14281 ggttactatt aataggagac aaatgcaagg ctttaaaagt aatcaagaag gcaagagaag
    14341 aaaaaatttt taacgaattt ctttttcgaa atatatgtta ttatatcatt ttggtttttt
    14401 ctattgtata tatgacaact atgttcaatt aaaatttaga aagtgagaaa ctattgtgta
    14461 tactattata aaatcaaata taaaaaaatt tagtttatta acgatattta ttgttgctgg
    14521 tcaattattg ctaatttatg cagcaactat taatgctctg gtgttgaatg aattaattgc
    14581 gatgaattta gagcggtttt tgaaattgtc aatctaccaa atgattgtct ggtgtgggat
    14641 aatattcctt gactgggtag tgaaaaatta tcaggttgaa gtgatccaag agtttaatct
    14701 agagattcga aatagagttg ccacagacat ctctaactct acctatcaag aatttcatag
    14761 taaatcatca ggaacatatc tttcgtggct aaataatgat gttcagactt taaatgatca
    14821 ggcttttaaa caactttttt tagtaataaa aggaatttct ggtactatat ttgcagttgt
    14881 gactcttaat cactatcatt ggtcattgac tgtagccacc ttgttttcat taatgattat
    14941 gctacttgta ccaaaaatct ttgcatcgaa aatgcgagaa gttagtctaa atttaactaa
    15001 ccaaaatgaa gcttttttaa aatctagtga gactatattg aatggatttg atgtgttagc
    15061 gtccttgaat cttttatatg tattgcctaa gaaaattaaa gaagcaggaa ttttattaaa
    15121 gatggttata caaagaaaga caactgtaga aacgttagca ggcgctatta gcttctttct
    15181 caatattttt tttcagatat ctctcgtttt tttaacaggc tatcttgcaa taaaaggaat
    15241 agtgaaaatt ggtactattg aagcaatagg agcactaaca ggtgttattt ttacagcgct
    15301 aggtgaatta ggaggtcaat tatcctctat tattggtacg aagcctattt ttttaaaatt
    15361 gtattcaatt aatccaattg agtcaaataa aatgaatgat atcgaaccaa atgaggtgaa
    15421 tagagatttt ccgttatatg aagcaaaaaa tatttgctat aagtatggag ataaagaaat
    15481 attaaaaaac ttaaattttt gttttcaacg taatgaaaag tatttaattt taggtgaaag
    15541 tggaagcggg aaatctacat tattaaaatt attgagtggc tttttgagag attatagtgg
    15601 agaattgcga ttctgcgggg atgatataaa aaaaacctcc tatttaaata tggtttcgaa
    15661 tgttctatat gtagatcaaa aagcttattt gtttgaaggt acgattagag ataatatttt
    15721 attggaagaa aattatactg atgaagaaat actacagtct ttagagcaag ttggtttgag
    15781 tgtaaaagat tttcctaata acattttaga ttattatgtt ggtgatgatg ggagattact
    15841 gtcaggaggg cagaaacaaa aaattacttt agctagaggg ctaattagaa ataagaaaat
    15901 agtattaatt gacgagggaa cttctgctat cgataggaga acttcgttag cgattgaacg
    15961 taagatatta gatagagagg atttgactgt cattattgtt acccatgctc cgcatccgga
    16021 acttaaacaa tattttacta agatatatca atttccaaag gattttattt aagaatatag
    16081 gggagggaaa catgagattc tattgtgaag cttatgaggt aattactgaa gagaaaaaag
    16141 tatactttaa tgatattgag ttagaagtta agtattcctc tgtcgaagaa ttgtttataa
    16201 tatgtgagaa attattagat aagaaaaaag tatcattttt ttatgtatat gagaaaccct
    16261 taaggtatct gttatttgac tatatatttc tactagttct agctaaaaaa aatatcccaa
    16321 tcttagatgg agtttccaat aagcaagttg atccaacgtt gctgcatcac ttttctttgg
    16381 aaattgaaaa gaattttatc gatttttgct ataaaaatat ggatctcatc ctaaaaactc
    16441 aaagtatatc tttgtgtcat agagaagagc ttattattgt agatgtttca gatgactcta
    16501 aatttggtgt tttttacaag cgttttagaa cactggttga taaaaataac ggaaaatatg
    16561 tttgtgttta taatttcagt cgtgtttcag atatactgca aaactggaaa aattattgca
    16621 atcgaaaatt ttcggttacg tttactgaaa gccaatttga attatttaag cttttatata
    16681 atcaaaaaaa ttttaaaaca atttcgttgc tatttggaaa aaaattgttg ctggaggaat
    16741 tatctattat agcgatctta ctaatataga atacttttgt attttttggt gggatagtat
    16801 gtttgggaaa gattctattg gaaaatacgt atatgtagaa gagattagta gatgtcattt
    16861 tttagataga aattattctt tttgttatgg tttacaagat tataaaagta aacttattaa
    16921 atatttttta gaatagttaa tctattcgag agagataaag aaatatggaa aatactttat
    16981 tgaatatcac aggaagcttc gatatggaaa ctaggaatct aattagttat tctttaactg
    17041 atatctttga aacagataaa ataagaattg aacttcttgg agaaatttat tataaaaata
    17101 taaagttgga attgcatgaa ttcgctggat tgtataagat ttatggaatt agcttaataa
    17161 agaatattac cggaatgttt ttaattataa tttttgatac gaagacaaaa gaattaaaaa
    17221 tttttcaaga tataacgact tcatacttta atttatacta tactgtttat ggaggtgtgt
    17281 tctatatata gtacatcttt aaagaaagtg atgaagttat ctcatgttcc cgttacctta
    17341 aataacaaaa aaatacaaga atttatgaga aatggattta ttttagattc aaatacttta
    17401 gtaaccgaaa ttaataaatt agaatatttc tcttacattt ctgtaaataa tactcttcgt
    17461 atatgtggta ttgattataa tgattcaaac aattttacta aagaacaagt attaaagaat
    17521 tgggactcta tgctacgtga atctatatta agagtgtata gtgaagcagg agaggctaat
    17581 attacactat catctggttt tgattcaaat tatatattat atacattagc gaattatacg
    17641 aattcttcaa ttaatgcatt ttgcatagga ggagaaaaag gaattaatga gattcctgaa
    17701 gtaactaaaa ttgcaaaatt ttatggtata aatttattag ttgatactgt aatgagccca
    17761 agatttagaa tattttcccg atatagtatg gagattggaa ggaagtctct ttgaatgtgg
    17821 tgtcatactg cagtactatt taggcaggtt gttgttccaa aaagggaaca caagtattct
    17881 ttgtggcgag tcagcggatg aaataatgac ttttaagtat cattctgtga actacaatca
    17941 attttgtaat gataaacaga agtcagtata ttttagttat tccgactatc cattttatgt
    18001 tacaaattca attgttttaa agaaaaatag cctacttttg catagttttg gcattcatcc
    18061 tagatatcca tataaaatgt ctgagattgt tgaaatgtct aaaaaaatat cagatctaaa
    18121 cgataaaaaa gaatttcaca aaaagaactg tgagttgagg tttcaagata gtgttcttga
    18181 taacataaat agtgtcccgg gtacaactca tttatttagt tgtttaaata ttcaaacatt
    18241 agtaaaaatt attttatata tttttcgata caacagttgt atgaaaatat ttaattttag
    18301 agataaagag cttatctttt ttaataagat tgtaaatggg ctgatacaaa atattgagga
    18361 gaatttagag gatgatattg aaaggatatt gaaatattta tatatatgtt tatttaacga
    18421 aatattcatt attaagaata aggtgaattt ttttgatgat gtggaattta atcagacact
    18481 tagtgagttt ttagataaat tgtagaggga atggttatga gtgacaatac taagtatatc
    18541 tttttatctc cacatttaga tgacgcaatt ttttcctgtg gtgattatat ttcaaaatta
    18601 acttcagaag gagaaattgt acttgttatt actatatttt ctggttaccc tttgagccaa
    18661 cagctacagc cctctgctaa gcaatttcat aagttgtgta accttgggaa atatccgatt
    18721 gaagaaagga agaaagaaga caggttagct tgtgaacgtc tacaatgtga ttttagacac
    18781 ttatcgtact atgaatgtct ctataggaaa gatagaaatg gcaattttct ataccgtcat
    18841 atctatagta aattaaaaaa tgaggacaca ttaaagaatg atatcataaa agagctgtta
    18901 atgcatttag acgacaaatg tgtcgtatat tgtccgcttt cacttggtga ccatgttgat
    18961 catgtttttg tgaatagtat tggaagggcg cttgaattta tgcgttataa agtaatatat
    19021 tatgaggact tcccatatgt aagtgatagt agtatggtta gttatatggg aaaaactaaa
    19081 gaattaaaaa tgtatcaaga ggaacttgat gaaaaacatt atattgatag aatatcatca
    19141 atattgtgct ataaatctca gattttgata atctggaaat cagttgaaaa acttttaaac
    19201 aatattaaag agttgtattt aaggaatggg gcagcttatt ctattagatt ttggattaag
    19261 aaataaggaa gtagttttat gaatatagca gtaattggtt tggggcatgt tgggctggcc
    19321 tacgcgttac tatttgcatc taaatataaa gttgttgcat atgatataga ttctgtaaaa
    19381 ataaataatt taaaaaaggg cactcttcca tctaaaaatg aagagcttat gaagtttttt
    19441 tgcgagaata acttaaatat tacttttttt gatacatttt ctgaaattaa aaataatatt
    19501 gattattata ttattgcgct tccgacagat tatgatgaga aaattggtag ttttaataca
    19561 tatgaaatcg aacaaacggt atcgaagatt ctgagggtaa aacctaatgg aaagattatt
    19621 ttaaagtcaa cagttccgat cggcttttca aacaaattaa aaaggctgtt tgatacaaaa
    19681 aatatcattt ttgtccctga atttttgaga gaaggttgtt ctatatatga taatttatat
    19741 ccaagtcgca tagttgttgg agatgagaca gttgaaggaa gaaaaattgc agagttgttc
    19801 ctttcgatta gtactcatag tactgccaat attaaaaatg ttatgttagt ttctcctact
    19861 gaagcagaag caattaagct tttttctaac acattcttag ctctccgtgt tgcttttttt
    19921 aatgaactag attcttttgc tgagaggaga agtttaaatg ctgaagttgt aataaaaggt
    19981 gtttgtttag atccaagaat tggaaatttt tataataatc cttcttttgg atttggagga
    20041 tattgtcttc ccaaagatac tagacaatta aaaaaagaat ttatagaaat aaatgcccca
    20101 gtgatagaag cgattgatat ttcaaacaca aatagaaaac agttcatagt taaacaaata
    20161 ttggaacgaa agccaaagat agtaggaata tataaattgg ggatgaaata taattcagat
    20221 aattacaaag agtcagctat tttaagtata attaatgaac tattgattgt cggtataaaa
    20281 attttagtat atgaaccaaa tttaaacgtt agtatagata acgtaatttt tgaaaagaat
    20341 tttgaactat ttataaaaca aagtgatcta attgttgcaa atagatggga taggggcctt
    20401 gaagcttata aagataaagt ttacacaagg gggatctgga taagagatta actattgata
    20461 ttaaaaaggc aataatttat aaaatttgta caaaaaaatg ttaaattttc acattttcat
    20521 agtatactgt ttaatagaaa gtaggttctt tgtaaccgct ccattataaa atcctttcac
    20581 tcttccggta tactattagg aaaaacataa ctggaggata ttttgagctc acacctcatc
    20641 gttccagtgg ctgttcccca agctcgtgta ctgcccaaaa aagttagaca ttctatttag
    20701 aacgaggatt gagttctgta ttgtacaagg ctcggtcctt ttagagtcag cttaaggctg
    20761 gctttttcaa tcaccaaagt gtcagaatgt tttgacaaat gaacacaaat aatgatataa
    20821 tatgcaaaag ctaggaggtg gtaggatgat tgaaaaaatg gaactggggg aattttacaa
    20881 ggaattgcgc ttggcgagaa agctcaagca gtcagatgtg gcttgtgctg gactaacagc
    20941 ctctcagttg tccaagtttg aactaggaca gtctatgctg tctgcagaca agctgatcct
    21001 agccatccaa gggatcaata tgaattttga tgagtttggg cacaaactca acaactatca
    21061 agaatctcca catatgcgaa ttggtagaag ggttgtggat tgcttttccc atcaggatat
    21121 agctgcttta gagcagctat tggaggaagt cgatcaagaa cagatggcgc agacctatcg
    21181 tcgtttgaat gccattgtga ttaaaaatgc actccattcc ttagataaaa gctacccgct
    21241 aacagaggag gatagcgagt ttttgactac ctacctctac gctattgagt cttggacctg
    21301 gtttgaactc tatctttttt gcaatactat gcccttcttg agcaatcaag atctgatctt
    21361 tttatcaacc tccttactcg aaaaatccaa ggaatttaaa gagttagtac acaatcgatg
    21421 gtatatgaaa agtggttatc ttaatatcat ctcagaactt atggagcgca agctattttc
    21481 ttatattcca atctttgaag ctgagctgga gcgcatgctc cgtccatacg atgtttttga
    21541 aaaagtattg tggcagtttt taaagaaaat gcagattttt cttcaaacca aaggaagcaa
    21601 tcaaaaagag attgaacact ttatccaatc tctgcaagta ttagaaaatc cacaattgac
    21661 agcccttttt gaattgcgtt tgcagcaata caaagagctt atcgattagt tagaaatggg
    21721 aaaaagataa taatatcagc aacgatcgtc aataatgtta aaacttgttt cttcatatta
    21781 cttccctcct cattagtaat aactttatta taccagggaa ataatcaaat ctaccaaaat
    21841 cgcaaatctg aaatttctat aagaaaaaat atcaaatatg cgattttcta aaataagcca
    21901 atttccgtgt tatactgtcc ttgtaaaaca tttgaagcaa atcctaggtc gcagaagtgg
    21961 tttacaaatg aagataattg aaggagtgta aaagatgtta aatttacaat ttgcagaaac
    22021 aatggaattg acagaagctg agttgcaaga tgttagagga ggcaacctcg taaattctat
    22081 gggaggtggt ggaagaagtg gtatatctgg ttggggcgtc ccaggaattt accctggttg
    22141 gggcaatcaa gggatgagtc ctaatcgtgg agcttttgat tggactatag atttagcaga
    22201 tggattgttt ggccgccgta gaagataggc tcttatagga gataatagga tgaagaaaat
    22261 gaaagaagtg aaatttcacc tagcaactgg cttgttgatt ttgacttatt acctcatttt
    22321 taatgtcaca tcagacttag attttatggt tgctttgtct gataatatgt actatgtgtt
    22381 ccaagtttta ttggtactta ttctgggaac tatagctaca attgcttttg tcaaaagtga
    22441 acattggaaa gagtgtggtc gtttccagtt tcgttggtct tacctgggtg ttttccttct
    22501 ttcttttttc ttattgttcg tgtgggcaaa tctaacaacc tatatctttc ctcgtaccca
    22561 gaatggaagt acagtcgtag aagtagccac cagtttaaca ggaattagct attttgttac
    22621 gcgaatactt tatactagta ttatcgcacc tgtatcagaa gaggttgttt gtcgtggtct
    22681 tttgatgaca agtttatcta aagttaagag atactatctt gatgttcttg tttccgctgc
    22741 catatttggc gctatgcatg ttttgcagta tggttggata acgacagatt tcataaagta
    22801 ttttggaatg ggcttgatat tttgtatgat gtttcgctac acccgatcca tctattgggc
    22861 tatagctctg catgcttcat ggaatagctt cttactcatt gttactttat tggtatttgg
    22921 atattagaaa ggtgatatat gaaaaagata atctcacacc gctactttat tatagttttt
    22981 ctactagtta ttgctgacca gaagttcagt gttttagttt tacgcagcga ccttgttgct
    23041 gggctatctg actttgccta ttatctgtcg gatatgatgt tgaattttct tgtggtttta
    23101 tttgctctta ttgctatgat ttggtcgggg aaatggcaga aaatcaatag tagaaagttt
    23161 aaaggatcct atcttttcta ttccttccta gctctgcttg cttttgttgc ttggaatttt
    23221 gtcacctttt ttcttttccc acctactcga aatgaaattt cttatcaaca tgctgctcct
    23281 acttttacag gagctacggc atttttgatg tatttttttt atcctgtgat tgcaggtccc
    23341 atttttgaag acatgattta tcgtggattg gtgatgacag ctctggaaaa aggaaagaaa
    23401 tgggggctag atgtacttgg ttctgctatt ttatttgggg tctcgcacat tagtaatcac
    23461 ggttgggtct tgacagactt tttcgtctat atgggtggcg gacttatatt tgcagtctta
    23521 tttagaatga caaagtccat ttattggcct attgggctgc atatagtcta caacggcatt
    23581 ggtcagcttt tgatgttact gtaattttgg ttgttagtag tatcgcggtc tatttctggg
    23641 gaaagattcc catttgaact taagaaggag ttgtgatgaa agtatttctt caaaatagag
    23701 attttaggca attaactatc aaccagtgga tttcaacgct tggggatacg attttttatc
    23761 tggccttcct gaattatgtg gcagatgcgt cttttgcccc tttggcgatt ttacttatca
    23821 cgatttcaga aacccttccc caagttctac aaatctttct gggagttttg gcggattttc
    23881 aacatcatcg tgtcttgaaa tacacagtca ttagttttac aaaatttttg ctttactcta
    23941 tagtgtccct atcgctttca gggcagtctt tttccttgtt gctagtaacc tttatttgtc
    24001 tgcttaacct cttgtctgac acattgagtt atttttcagg cgccatgctc actccgattt
    24061 tcattagaat tattgggcaa gaccatctgg cagaagctat tggatttaaa cagtcaactg
    24121 ttagtttagt gaaaacaatc agtaatatcc taggaggagt cttactaggc attctatcca
    24181 tccagtttat ttccttactg aacgctctta cctttttaat cgcattttta ggtatcctct
    24241 tcataaaaac tgacctcttg aaagtagaaa aaacgattag ctatcaagaa ggactctctg
    24301 taaaatcctt ttgccagcat ttgctccaat catcgaaatt gatatggaat atgaataagg
    24361 tgctcttggt tttgtttatt atctctacta gtcaagcagt gataaatgtt acggttccta
    24421 tttccactct atttttaagg aaccagccct ttttgaattt acaaactggt caatctcttg
    24481 ccttgttagc cacactagaa ttatcagccc ttattttggg aagtctgatg agtggctatc
    24541 tgcaacatac catttccata aaaatagccc tgtatgcttc actcctactc caattactac
    24601 tcctagtggg ctttgcgaca gctcattttg gcttgattct gatctttagc tttttggatg
    24661 cctttttcgc gggtattctc tctcctagat tgcaagaact cgtctttaaa caaatacctg
    24721 aggagtcaat gggagcagtt caatcttcta tcggtgccat tacggttgtt ttacctagct
    24781 tatttacaat agctttggga accattgcta ctagctttgg aactctggca gttagctttg
    24841 ttttattact atttcttcta gttgcctttg tcatgcttct gaatattcgt gaaagtattt
    24901 agtggagaat ttctataata tatgtttgag actggattgc cagtcttttt atgatggtca
    24961 aaatcaacag actttttctc ttttctgaat gtgttatagt agactgaatc taaaatagta
    25021 cgaaataatt gctaaaacat ttatagaaat taattttact ttcccaatcg atttgttctc
    25081 atcttatttc aatctgctat ataatagtcc atgctgtggc tggattcttt tcagcctact
    25141 tatactcttc gaaactctct tcaaaccacg tcagcttcac cttgccgtag gtatggttac
    25201 tgacttcgtc agttctatcc acaacctcaa aacagtgttt tgagctgact tcgtcagttc
    25261 tatctgcaac ctcaaaacaa tgttttgagc aacctgcggc tagtttccta gtttgctctt
    25321 tgattttcat tgagtattat taagacaata tgaggagaaa gatgaaagaa aaaggatttt
    25381 aggagggggc gcaggcagcc atgccaacgg ctcttggcta tgtcagtatc ggcctggcct
    25441 gtgggactat cggtgcgccc tatgtgacac ctgttgagat gggcttgatg agtctctttg
    25501 tttatgctgg gagtgcccag tttgccatgt tggcactgat tgtggttcaa gctcctgtgg
    25561 cagctattgc tatgacggtt tttctaatca acttgcgtct ctttttgttg agtttacacg
    25621 catcgactta tttccgtcat accagtcttt ggtacaatat cggtatgtct agtatcttga
    25681 cagatgagac ctatggcgtt ttgatgggtg aattggccca tacagacaag gtaaatccta
    25741 tgtggatgca cggaaacaat cttaacagct atgtggcttg gtttgtgggg acagtagtcg
    25801 gaacggctct gggtggcctg ctaccaaatc cagaaatctt tggcttggat tttgccctgg
    25861 ttgggatgtt tattggtatt tttgcttcgc aatttcagat tatgcaaaga cggattcctg
    25921 tccgcaatct gctcattatc ctagcagttg ttgcggtgtc cttctttttg ctcttgacag
    25981 tggtgtctca gtcactagct gttctgtttg cgacgctact tggttgtagc atgggggtgg
    26041 ttttagatgg tcagtaagta tcttttatta gcagttattt tctctgactt ggtgacttgg
    26101 attctccgta tgattccctt catcttagtc aagtataagg gcttgcctgc aatcgttgag
    26161 cgttttttga agttcttgcc cgtttccatt atctttgcct tgattctttc aagcgtagtg
    26221 acaggtaagg ttgggagcct tcctcaaatt aaatggctag acttcttagc cgtctttcca
    26281 acagcttggg tagcctttcg ctaccgcaat ctagtcggaa cagttctctt tggagtagtc
    26341 ttgattgccg tcttgcgttt ggtcttttaa atcagccata aagaaaacct atcacagata
    26401 tatatatcat ataatggtgt aaatgatttt tttctgttaa gattataagg tattctattt
    26461 tggaggaaat gacatgaaaa aaatcgttaa atactcatct cttgctgccc tagggcttgt
    26521 tgctgcaggt gtgctagcag cttgctccgg tggtgctaag aaagaaggac aagcagctag
    26581 caagaaagaa attattgttg caactaattc ttcaccaaaa ccattcaact atgaagaaaa
    26641 tggcgaattg actggttacg agattgaagt cgttcgcgct atctttaaag actctgataa
    26701 gtatgatgtc aagtttgaga agacagagtg gtcaggtgtc tttgcgggtc ttgacgctga
    26761 ccgttaccaa atggctgtta acaacatcag ctacactaaa gaacgtgcgg agaaatacct
    26821 ttatgcagcc ccaactgtta aagaccctaa cgttcttgta gtcaagaaag acgattctag
    26881 catcaaatcg cttgatgatg ttggtggaaa atcaacagaa gttgttcaag gaacaacatc
    26941 tgctaaacag ttagaagact acaacaaaca acactcagat aatccaacta tccttaacta
    27001 tactaaagca aacttacaac aaatcatggg ccacttgagc gatggtcagt ttgactacaa
    27061 gatttctgat aaaatcactg ttgaaacagt catcaagaac caaggtttgg acaacttgaa
    27121 agtcatcgat cttccaagcg accaacaacc ttacgtttac ccacttcttg ctaaaggtca
    27181 agatgagttg aaaacatttg tagacaaacg tatccaagaa ctctacaaag acggaactct
    27241 tgaaaaattg tctaaacaat tcttcggaga cacttaccta ccggcagaag ctgatattaa
    27301 ataatttctt gaaatcatcc attctgagta agatgggtga tttttcagtt cgctaccgca
    27361 atctagtcgg aacagttctc tttggagtgg tcttgattgc catcttgcgt ttggtctttt
    27421 aaattaccca taaaaaaaac ttatcacaga gatagatatc atataatggc gtaaatgctc
    27481 tttttctgtt aagattataa ggtattctat tttggaggaa atgacatgaa aaaaatcgtt
    27541 aaatactcat ctcttgcagc ccttgctctt gttgctgcag gtgtgcttgc ggcttgctca
    27601 ggtggtgcta agaaagaagg acaagcagct agcaagaaag aaatcatcgt tgcaaccaat
    27661 ggatcaccaa agccatttat ctatgaagaa aatggcgaat tgactggtta tgagattgaa
    27721 gtcgttcgcg ctatctttaa agattctgac aaatataatg tcaagtttga aaagacagaa
    27781 tggtcaggtg tctttgctgg tcttgatgct gaccgttaca atatggctgt caacaatctt
    27841 agctacacta aagaacgtgc ggagaaatac ctctatgccg caccaattgc ccaaaatcct
    27901 aatgtccttg tagtgaagaa agatgactct agtatcaagt ctctcgatga tatcggtgga
    27961 aaatcgacgg aagtcgttca agccactaca tcagctaagc agttagaagc atacaacgct
    28021 gaacacacgg acaacccaac tatccttaac tatactaagg cagatttcca acaaatcatg
    28081 gtacgtttga gcgatggaca atttgactac aagatttttg ataaaatcgg tgttgaaaca
    28141 gtgatcaaga accaaggttt ggacaacttg aaagttatcg aacttccaag cgaccaacaa
    28201 ccgtacgttt acccacttct tgctcagggt caagatgagt tgaaatcatt tgtagacaaa
    28261 cgcattaaag aactctacaa agacggaacg cttgaaaaat tgtctaaaca attcttcgga
    28321 gacacttatc taccggcaga agctgatatt aaataatttc ttgaaatcat ccattctgag
    28381 taagatgggt ggtttctcag tttttaggga tatagtttta aactatatat ctgactatct
    28441 aataacaatt tgtgaaatca aacaaattgt gagatactag tacggtatta tttttaagga
    28501 gaaagaatca tgaaaatcaa aaaatggctt ggcctagcag cccttgctac agtcgcaggt
    28561 ttggctcttg cagcttgcgg aaactcagaa aagaaagcag acaatgcaac aacgatcaaa
    28621 atcgcaactg ttaaccgtag cggttctgaa gaaaaacgtt gggacaaaat ccaagaattg
    28681 gttaaaaaag acggaattac cttggaattt acagagttca cagactactc acaaccaaac
    28741 aaagcaactg ctgatggcga agtagatttg aacgctttcc aacactataa cttcttgaac
    28801 aactggaaca aagaaaacgg aaaagacctt gtagcgattg cagatactta catctctcca
    28861 atccgccttt actcaggttt gaatggaagt gccaacaagt acactaaagt agaagacatc
    28921 ccagcaaacg gagaaatcgc tgtaccgaat gacgctacaa acgaaagccg tgcgctttac
    28981 ttgcttcaat cagctggctt gattaaattg gatgtttctg gaactgctct tgcaacagtt
    29041 gccaacatca aagaaaatcc aaagaacttg aaaatcactg aattggacgc tagccaaaca
    29101 gctcgttcat tgtcatcagt tgacgctgcc gttgtaaaca ataccttcgt tacagaagca
    29161 aaattggact acaagaaagc acttttcaaa gaacaagctg atgaaaactc aaaacaatgg
    29221 tacaacatca ttgttgcgaa aaaagattgg gaaacatcac ctaaggctga tgctatcaag
    29281 aaagtaatcg cagcttacca cacagatgac gtgaaaaaag ttatcgaaga atcatcagat
    29341 ggtttggatc aaccagtttg gtaataagaa acagggaggt gggagagaaa attccacctc
    29401 ttgcttttgt atagagtata gattgtaaag aagactattc gttcatagaa aggtagagag
    29461 aatatggttt ttcctagcga acaagaacag attgaaaaat ttgaaaagga tcatgtagtc
    29521 caacattatt ttgaggtttt gcgtaccttg atttctaaga aatcagtctt tgcccagcag
    29581 gttggactca aggaagtcgc aaattatctg ggtgagattt tcaagcgtgt tggagctgaa
    29641 gtggagattg atgagagcta tacagcgccc tttgtcatgg cacatttcaa gagttcgcgt
    29701 ccagatgcca agaccttgat tttctataac cactatgaca ctgtgccagc ggatggggat
    29761 caggtctgga cagaggatcc ttttacgctt tcggtccgca atggcttcat gtatgggcgt
    29821 ggggttgatg acgacaaggg tcatatcaca gctcgcttga gtgctttgag aaaatatatg
    29881 cagcaccatg atgatttacc tgtcaatatc agctttatca tggagggagc ggaggaatcg
    29941 gcttcaacag acctagataa gtatttggaa aagcatgcag acaaactccg tggggcggat
    30001 ttgttggtct gggaacaagg gaccaaaaat gccttggaac agctggaaat ttctggtggc
    30061 aataagggga ttgtgacctt tgatgccaag gtaaaaagcg ctgatgtgga tatccactcg
    30121 agttatggtg gtgttgtgga atcagctcct tggtatctcc tccaagcctt acagtctctt
    30181 cgtgctgcgg atggccgtat cttggttgaa ggcttgtacg aagaagtaca agagcccaat
    30241 gaacgagaaa tggccttgct agaaacttat ggtcaacgaa acccagagga agttagtcgg
    30301 atttatggat tggagttgcc tctcttacag gaggagcgga tggcctttct aaaacgtttc
    30361 tttttcgagc cagcgcttaa tatcgaagga atccagtctg gttatcaagg tcagggtgtt
    30421 aagactattt tacctgcaga agccagtgcc aagctagagg ttcgtctggt tccgggccta
    30481 gaaccgcatg atgttctgga aaaaattcgg aaacaactag acaaaaatgg ctttgataag
    30541 gtagaattat actatacctt gggagagatg agctatcgaa gcgatatgag cgcaccagcc
    30601 attctcaatg tgatcgagtt ggccaagaaa ttctatccac agggcgtttc agtcttgccg
    30661 acgacagcgg ggacaggacc tatgcatacg gtctttgatg ccctagaggt accaatggtt
    30721 gcatttggtc taggaaatgc caatagccga gaccacggtg gagatgaaaa tgtgcgaatc
    30781 gctgattatt acacccatat cgaattagta gaggagctga ttagaagcta tgagtagaga
    30841 tattatcaag ttagatcaga tcgatgtgac ttttcaccaa aagaagagaa ccatcacagc
    30901 ggttaaggat gtgaccattc acatccaaga aggggatatc tacggaatcg ttggatattc
    30961 tggagcaggg aaatcaaccc ttgtacgggt gattaacctc ttgcaaaaac catctgcagg
    31021 gaaaattacc attgacgacg atgtgatttt tgatggtaag gtcaccttga cggcagagca
    31081 gttgcgtcgt aaacgtcaag atatcgggat gattttccag cattttaacc tgatgagcca
    31141 aaagacagca gaggagaatg tagcctttgc ccttaaacac tctggactca gcaaggaaga
    31201 aaagaaggct aaagtagcta agttgttgga cttggttggt ttggcagatc gtgctgaaaa
    31261 ctacccttca caactatctg gagggcaaaa acagcgtgtg gcaattgcgc gtgccttggc
    31321 caatgatcca aaaatcttga tttcagacga gtcaacttct gcccttgatc cgaagacaac
    31381 caagcagatt ttggccttgt tgcaagattt gaaccaaaaa ttaggcttga ctgttgtctt
    31441 gattacgcat gaaatgcaga ttgtcaaaga cattgccaac cgtgttgcag ttatgcagga
    31501 tgggcatttg attgaagagg gtagtgtgct tgaaatcttc tcaaatccta aacaaccttt
    31561 gactcaagac tttatctcaa cagctacagg tattgacgaa gccatggtca aaatcgagaa
    31621 gcaagaaatc gtggaacact tgtctgaaaa cagtctcttg gtgcaactca agtacgctgg
    31681 agcttcaaca gacgagccac ttttgaatga attgtacaag cattaccaag taatggctaa
    31741 tattctctat gggaatatcg aaattctcga tggtactcct gttggagaat tggtggtggt
    31801 cttgtcaggt gaaaaagcag cgttggcagg tgcccaagaa gccattcgtc aagcaggtgt
    31861 acaactaaaa gtattgaagg gaggacagta agatggaatc attgattcaa acctatttac
    31921 caaatgtcta taaaatgggt tgggctggtc aggcaggctg gggaacggct atctacttaa
    31981 ctctttatat gacagttctt tccttcatta tcggaggctt cttggggcta gtggcaggtc
    32041 tctttctcgt cttgacagcg ccaggtggtg tcttggagaa taaagtcgta ttctggattt
    32101 tagacaaaat tacctcaatt tttcgtgcag ttccctttat catcctcttg gcaatcttgt
    32161 caccactttc tcacttgatt gttaaaacaa gtatcgggcc aaatgcagcc cttgtcccac
    32221 tttcttttgc agtctttgcc ttctttgccc gtcaggtgca ggttgtcttg gctgaattgg
    32281 atggcggtgt cattgaggcg gctcaagcga gcggagcgac tttctgggac atcgtgggtg
    32341 tttacctatc agaaggtctt ccagatttga tccgtgtgac gactgtgacc ttgatttccc
    32401 ttgttgggga aacagctatg gccggtgcgg ttggagctgg tggtatcggt aacgtagcca
    32461 tcgcttatgg atttaaccgc tacaatcacg atgtgaccat cttggcaacc atcattatca
    32521 ttttgattat ctttgcaatc caattcttgg gagatttctt gactaagaaa ttgagccata
    32581 aataaaaaag agccgcgtgg ctctttttaa ctgatcagat tttctgtgca aattttttac
    32641 tcaaggcttg tccaatcaag gcacccacta gggctccgat gacaatactt gcgataaata
    32701 gaaggacagt tccagggttt ggagcgacca tgatgcggtc gatatattct tgggattttc
    32761 ctcttgccag aagagtagcc atataggctt tgggcgcaat ccacataagc aagattggtc
    32821 ctgttgtact aaaggcgaaa ataatgaaag aaaggaagtt ctttgttttg tccttgtatt
    32881 ttcctaaatg agctactcca tctgctagga ggccacagat aattccagga aggaaggaac
    32941 cagcaccgtg tttagttccc aagaaaaaga gggcaatgac aaggccgata gtggtaatgg
    33001 ctccaaagcg cggaactttt gcgactagga tcatatagac gctaccgccg acaagggcag
    33061 taaaggcagg cgcataaaac atgtttccag tttggtcaaa gagattgccc aaaaggacac
    33121 caatccccat gcagaggaag taaagaactg caaaaagcag tgtagttaag atagatttct
    33181 tcatgaattg tctcctgata attttttcac aattctcatt ttaccatggt ttggtgggat
    33241 tgtaaatgga tactagaagt ttttgaaaac gcttgaaata tgatataatg gtttcatagt
    33301 tatttttagg ggaatatcat gggtagattt ttagactttg tctttaatcg tttcttttta
    33361 gggatgattg cgacagcctt cttttggcta ttaactttag caggagggat tatccttggt
    33421 ctagcgccgg ctagtgccac cttgatgagc ttatatgcag aacatggtta tagctttcgg
    33481 gaatacagtt tgaaggaggc ttggtctctt tacaagcaaa attttgtctc aagcaacctg
    33541 attttctata gctttttagg tgtgggtcta gttttgacct atggtttgta tctcttggtg
    33601 caattgcctc atcagactat tgttcatttg attgcgaccc ttttgaatgt cctagtagtt
    33661 gccctgatct ttttggctta tacagtatct ttaaaattac aagtttattt tgccttgtcc
    33721 tatcgaaata gtctcaaatt atccttgatt ggcatcttta tgagtctagc agctgtggcc
    33781 aaggttctcc ttgggactgt gctacttgta gcaattggtt actatatgcc tgccctgcta
    33841 ttttttgtag gaattgggat gtggcatttc tttatcagtg atatgttgga acctgtctat
    33901 gaaatcatcc atgaaaaatt ggcgacaaaa tagaatgaag cagttttggc tacatacgct
    33961 tctaagaacc tatagttcag tgatgatcat tatcattgcg agttttgcaa tcttactctc
    34021 ttacgctgac tgggattcac gtgaaaagga agcccagaga gtagcccagc gtgtaactgc
    34081 tagaacagtg agtgaaattg aatattacca tagagagtca acccagatag ctcaggcttt
    34141 agttgaaaat caagctcgta ttgagggaat ctataaatac tttagcctta gcatgccaga
    34201 ctatttttac tggcaattag agcggaaagc ttcgccttat atatcagtct ctctgtatga
    34261 aaatgttgat gacctctatg ttcgaaatga ttttgtaact ggggtggcca ttgcttttca
    34321 agattacaag gaagtctatg tttctactaa agacaaacgt agtggagaaa aaatcagggc
    34381 tgaggatttc aaaccagcag gaaatagttt tgccattccg gtgtcagatc cagtgtcaga
    34441 tcaagattta ggagtgattt acatctcctt ggatcctgct gttttatacc atgccattga
    34501 taatactaga ggtcatactc cgatggcagt aacagtgacc tcaccttttg atacggagat
    34561 ttttcatatt ggtgagacag ttgataggga gagtgaaaat tggctagttg gcttaacttc
    34621 tcatggatat caggttcagg tggcagttcc taaaaacttt gttttacaag gaacagtgac
    34681 tagctctgct ttgattgtgg gtttgagcct tctctttatt gtcattcttt atctgacttt
    34741 gaggcagact tttgctaatt accaaaagca ggtagtggat ttagtggaat ccattcaagt
    34801 cattgctcaa ggcgaagagg ggcgtcggat tgacatttcc gaaaaagatc aggaattact
    34861 cctaatcgcg gagacgacca atgatatgtt ggatcgattg gaaaagaata tccatgatat
    34921 ttaccagtta gagcttagtc aaaaagatgc caatatgcga gccttgcagg cgcaaatcaa
    34981 tcctcatttt atgtataata cgctggagtt cttgcgcatg tatgcagtta tgcagagtca
    35041 agatgagttg gcagatatca tttatgaatt cagtagtctc ttgcgtaaca atatttccga
    35101 cgaaagagag accctcctca aacaggaatt agaattttgc cgtaaataca gctatctctg
    35161 catggttcgc tatcccaagt ccattgccta tggtttcaag atagatccag agttagagaa
    35221 tatgaagatt cccaagttta ccttgcaacc gctggtagaa aactatttcg cgcatggtgt
    35281 tgaccacagg cggacagata atgtgattag catcaaggct cttaaacagg atggttttgt
    35341 ggaaattttg gtggtcgata atggtagagg aatgtcggct gaaaagttgg caaatatccg
    35401 agaaaaatta agtcagaaat attttgaaca ccgagccagc tacagtgatc aaaggcagtc
    35461 tatcgggatt gtcaatgtac acgagcgttt tgtgctctat tttggagacc gctatgccat
    35521 tacgatagag tctgcagagc aagccggtgt tcagtatcgt attacaattc aagatgagta
    35581 gaaagggaga aaatgtataa agtattatta gtagatgatg agtacatggt gacagaaggt
    35641 ctgaagcgtt tgattccctt tgataagtgg gatatggagg tcgtcgcaac agccagtcat
    35701 gccgatgaag ctctagaata tgttcaggaa aatcctgtcg atgtcatcat ttccgatgtc
    35761 aatatgccag acaaaacagg gcttgatatg attcgggaga tgaaagagat cttaccagat
    35821 gctgcctata tcctgctctc aggttatcag gagtttgatt atgtaaaaag agcaatgaac
    35881 cttagtgtgg tggactattt ggtcaagcct gttgataagg tagagctggg aaatctgctg
    35941 gagaagattg caggtcagct cggcgagaga gggaagaaaa gtcagactct tagtcaagaa
    36001 ttagacgagg ctggatttgt tagttattta ggggataagg agaattggtg gataggtcta
    36061 tccaaggaaa aacaaggttc cttcaccatt ccctactatg tcttgggcca agactggcag
    36121 attttcattt ctgaccaacc cctagatggt ttagtcgtta caccttttga agctccttat
    36181 caagaacact ttgaacgctg gaagctgaat gctgagaaaa ccctctttta cggttctgta
    36241 aatttgcagc agtctgagag tctctttgcc tattacgaac cgatttatag ggttatcatt
    36301 cagggaaatc tcaatcaaat cgtagaagag ttaaatctct tggagaaggt agttcttgaa
    36361 aatacgccgc gtgttccgat tactaaacag ctttttattc agtttgtcat ggatgttttc
    36421 catttatttg aacatctcaa agctgatgac ctgacagaca tcgtcaaaac cattcatgct
    36481 attcaatcct tcgatgaatt ggttccttat atcaaggaaa ctctgagcag ctttttcggt
    36541 caataccgta tgaatgaaaa tgtggtcagt gtgctggaag tcattggttg tgattaccaa
    36601 aaagagcttt ccctcaagga tatcagtaag gctctcttta tcaatcctgt ctatctaggg
    36661 cagttgatta agcgtgaaac cgattcgact tttgcagagt tactaaacaa acaacgtatt
    36721 aaggctgccc aacaactttt gctttcaact agtgacagca tcgaagatat ttgttatgct
    36781 gttggttaca gtaaccttgg atatttctat aaagttttcc gaaaattgtg cggaaaatcg
    36841 ccaaaagcct accgaaaaca ggtagaaact atactataag atttgtattc ctttacaaaa
    36901 tgtgctataa tataaatatt attattagga ggttctatca tgaaaaagaa accaatttat
    36961 ctatgggtct tgctaatctt gtctgccctt atttcagttc cgtctctgtt tggaattgtg
    37021 agtcccttgc ctagtaaaga agcccttcgt actgctcaga aacaagttgc aggggtcaat
    37081 gctcagcaat tagaagacca gcttaattat acctatagag tagcagaagc atctcattct
    37141 atttttaatg ttgctttgat tgtgctatct actattttag tggtcgtagc gattgtcttc
    37201 cttgttcgta aaaatttgca atatgcaaac tatacttacg ttggctatgt tttgttagct
    37261 attattggtt cgatttatgg ctatgttggt ttacaagacg ctgtgcagtt ggttcaagat
    37321 gagagcatgc gtttgacagt gagtattggt tcaaaagctg ttagcatttt ctatatcgtc
    37381 atcaatgttc tcttcctagc ccttgtcttc tataagatgt ggcgacaaca gaaagcctcg
    37441 gcagaagaag aggaaacaga agagcttgcc taagtattga caaaaaagaa ctatcaagat
    37501 acaatcttgg tagttgtttt ttgattatag ataaaatcat ggaggtacat atgaagataa
    37561 tttctttagt ttatattagt ttgagtggca acactgaaag ttttgtgacg cgcttgaaag
    37621 actatctctt gtcccagtac aaagggattg aggttcaaaa gattcatatc aaggatttag
    37681 tcaaggaagg caaaaatttc tatgaaatgg accatcccta tgtcgctttt ttgccgacct
    37741 atctcgaagg tgggaatggc gtggataacg gagatgttga gattttgacg acaccggtgg
    37801 gagattttat tgcctatggt aacaatgcta gtaagtgttt tggtgtggtt gggtcaggaa
    37861 atcgtaactt taataaccaa tactgcctaa cagccaagca atacagtcaa cgctttggct
    37921 ttcctgtatt ggctgacttt gaaatgcgag gcatgctgga agatatcaaa catgtcgcag
    37981 ctattatcgc agatttgtat gagttggaaa aggagaatta atactcaatg aaaatcaaag
    38041 agcaaactag gaagctagtc gcaggttgct caaagcacag ctttgaggtt gcagatagag
    38101 ctgacgtggt ttgaagagat tctcgaatag tataaaattc ttataagagg tggttgaagt
    38161 tgttcaactg ttttttgagt ataaacagtc tttaagaacg taaaaaagca taagttcagg
    38221 tcttcaaaac cttgttctta tgctttttat catagaaata tttgctgaat tttctcttga
    38281 aatgaaacct tatcttcatc tcgcgcttgt cccttaccct gcttttccga ttgcgagtgc
    38341 gtagataaag aagatggcga agccaaagag gaaaatcaat cccgcaatgg caaggtgttt
    38401 gagccaagaa ggaagatttt tgttttcacg cgttaccaag gcgtaattca aaagagcaaa
    38461 gaaaggtgtt gtcaggaaag agccaatcat ggcaaagcgg agcatggttg aaacctgacc
    38521 agagaagaac ttgataatga cgataccgat gatagcagtg atggtcatcc agatgttcaa
    38581 agatttacga ttgtcctctt tttgactgat tagcagtcgg agagattcct gattaacgcg
    38641 agaatagcca tcgataacag ttataactgt tccaaagata cagaggaagg caataaaggt
    38701 aatcaagtaa cgggaccatt cgccaagaac agaggcatac atgcccacga attgagagat
    38761 gtatttggct gaagcagctt caaccgcctg ccctgtagga tactgaatca gtgctcccag
    38821 tgccacaaag aagacggcta ggatagctgt tccaatataa ccagtgttaa agtcaaacag
    38881 agcgtcttct gtgttaaagt tgacggtctt tctcttttca gctgaccaaa gtgaattgat
    38941 ggctgaaatt tcaataggag ccggcatcca tcctaggagg gagacgatga agggcagagc
    39001 tgccatttgc caaggtgtct tctcgacaaa atcagaactg tattctggat gcttgaccgc
    39061 cgcaatgata actgccagaa cagtcgcaat ggttaaggca gacataatcc atttgaccat
    39121 gccgtctaaa agtttgtagc ctccaaagag tagcatagcc caaatgattg caacgagaat
    39181 gagggaccac tgagtaatgc taagtccaat cattgggaag gcactggcga tgatagctga
    39241 gcacagaatg gcaacaccag ccgtgttgac catagccgaa aagacattga ggataaagaa
    39301 aatccagaga tagagttttc ctttttcggc ataaccttca accaaagtct ttccagtatc
    39361 agctgtgtat tcagcaccaa aacggaaaaa tggatattta aagacattgg ctaagatgac
    39421 caagagaagt agagaccaac cgtaagaacc gccagcttga gttgaggata caatgtggga
    39481 acctccaaca gcggcagttg ccattaggat tccaggtccc attgctttta gcttgcttgc
    39541 ccaggttgat tgatttaagg aaatagcttg cgacatgata aatactcctt tttgaatttt
    39601 cagaataatc tgtatatgta atctatattc tacaaaaaat tttggaaaaa tgcaagaata
    39661 ttttgaaaaa agatagaaaa tttacaaaga aaat
//
LOCUS       DAVBBS010000013        37669 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVBBS010000013 DAVBBS010000000
VERSION     DAVBBS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37669
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.013"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            108..896
                     /gene="codY"
                     /locus_tag="VQU32_000663"
     CDS             108..896
                     /gene="codY"
                     /locus_tag="VQU32_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HET2472151.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            896..1471
                     /locus_tag="VQU32_000664"
     CDS             896..1471
                     /locus_tag="VQU32_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET2472152.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDFFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            1553..1638
                     /locus_tag="VQU32_000665"
     tRNA            1553..1638
                     /locus_tag="VQU32_000665"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1587..1589,aa:Leu,seq:aag)
     gene            1921..3051
                     /gene="ugpC"
                     /locus_tag="VQU32_000666"
     CDS             1921..3051
                     /gene="ugpC"
                     /locus_tag="VQU32_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET2472153.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(3198..3344)
                     /locus_tag="VQU32_000667"
     CDS             complement(3198..3344)
                     /locus_tag="VQU32_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472154.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            3552..4310
                     /locus_tag="VQU32_000668"
     CDS             3552..4310
                     /locus_tag="VQU32_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET2472155.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGLSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKVLTGEMTLMKLSGMIYDEGWLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            4397..4909
                     /locus_tag="VQU32_000669"
     CDS             4397..4909
                     /locus_tag="VQU32_000669"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET2472156.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            5091..6035
                     /locus_tag="VQU32_000670"
     CDS             5091..6035
                     /locus_tag="VQU32_000670"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HET2472157.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            6044..6721
                     /locus_tag="VQU32_000671"
     CDS             6044..6721
                     /locus_tag="VQU32_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HET2472158.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVRSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            6819..7577
                     /locus_tag="VQU32_000672"
     CDS             6819..7577
                     /locus_tag="VQU32_000672"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET2472159.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            7615..9120
                     /gene="lytC"
                     /locus_tag="VQU32_000673"
     CDS             7615..9120
                     /gene="lytC"
                     /locus_tag="VQU32_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HET2472160.1"
                     /translation="MSVTFFIGEERLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSTEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAESDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTGTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPHYSGKKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            9616..10134
                     /locus_tag="VQU32_000674"
     CDS             9616..10134
                     /locus_tag="VQU32_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HET2472161.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            10264..10770
                     /locus_tag="VQU32_000675"
     CDS             10264..10770
                     /locus_tag="VQU32_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET2472162.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            10770..10940
                     /locus_tag="VQU32_000676"
     CDS             10770..10940
                     /locus_tag="VQU32_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472163.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            10972..12204
                     /gene="clpX"
                     /locus_tag="VQU32_000677"
     CDS             10972..12204
                     /gene="clpX"
                     /locus_tag="VQU32_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET2472164.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            12213..12800
                     /locus_tag="VQU32_000678"
     CDS             12213..12800
                     /locus_tag="VQU32_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HET2472165.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            12811..13191
                     /locus_tag="VQU32_000679"
     CDS             12811..13191
                     /locus_tag="VQU32_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET2472166.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            13243..14133
                     /gene="rapZ"
                     /locus_tag="VQU32_000680"
     CDS             13243..14133
                     /gene="rapZ"
                     /locus_tag="VQU32_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET2472167.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            14130..15107
                     /locus_tag="VQU32_000681"
     CDS             14130..15107
                     /locus_tag="VQU32_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HET2472168.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            15104..16015
                     /gene="whiA"
                     /locus_tag="VQU32_000682"
     CDS             15104..16015
                     /gene="whiA"
                     /locus_tag="VQU32_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HET2472169.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLVNGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(16049..17017)
                     /locus_tag="VQU32_000683"
     CDS             complement(16049..17017)
                     /locus_tag="VQU32_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET2472170.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWTLAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            17153..18010
                     /locus_tag="VQU32_000684"
     CDS             17153..18010
                     /locus_tag="VQU32_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HET2472171.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            17997..18776
                     /locus_tag="VQU32_000685"
     CDS             17997..18776
                     /locus_tag="VQU32_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HET2472172.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            18800..20152
                     /locus_tag="VQU32_000686"
     CDS             18800..20152
                     /locus_tag="VQU32_000686"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET2472173.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAIQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            20226..20600
                     /locus_tag="VQU32_000687"
     CDS             20226..20600
                     /locus_tag="VQU32_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HET2472174.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            20602..21450
                     /locus_tag="VQU32_000688"
     CDS             20602..21450
                     /locus_tag="VQU32_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2472175.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            21727..22494
                     /locus_tag="VQU32_000689"
     CDS             21727..22494
                     /locus_tag="VQU32_000689"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET2472176.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDDMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            22506..23690
                     /locus_tag="VQU32_000690"
     CDS             22506..23690
                     /locus_tag="VQU32_000690"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET2472177.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            23687..25558
                     /locus_tag="VQU32_000691"
     CDS             23687..25558
                     /locus_tag="VQU32_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2472178.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDSKIRPFFGHYTDYLDEKAFETDMANQVQKVEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(25701..26885)
                     /gene="mntE"
                     /locus_tag="VQU32_000692"
     CDS             complement(25701..26885)
                     /gene="mntE"
                     /locus_tag="VQU32_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HET2472179.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPISEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            27189..29885
                     /locus_tag="VQU32_000693"
     CDS             27189..29885
                     /locus_tag="VQU32_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET2472180.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            29898..30689
                     /gene="yghU"
                     /locus_tag="VQU32_000694"
     CDS             29898..30689
                     /gene="yghU"
                     /locus_tag="VQU32_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HET2472181.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            30805..31215
                     /locus_tag="VQU32_000695"
     CDS             30805..31215
                     /locus_tag="VQU32_000695"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET2472182.1"
                     /translation="MEKKIVKDILFLSQVSHPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGMQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            31227..32822
                     /locus_tag="VQU32_000696"
     CDS             31227..32822
                     /locus_tag="VQU32_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472183.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
     gene            32989..33153
                     /locus_tag="VQU32_000697"
     CDS             32989..33153
                     /locus_tag="VQU32_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HET2472184.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            33232..33960
                     /gene="yaaA"
                     /locus_tag="VQU32_000698"
     CDS             33232..33960
                     /gene="yaaA"
                     /locus_tag="VQU32_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HET2472185.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(34011..35495)
                     /locus_tag="VQU32_000699"
     CDS             complement(34011..35495)
                     /locus_tag="VQU32_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2472186.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILERLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEGYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYISGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
     gene            35711..>37669
                     /locus_tag="VQU32_000700"
     CDS             35711..>37669
                     /locus_tag="VQU32_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2472187.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAVSVLLGTALVLGAAASV
                     QTVQAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTGEV
                     TKPAEKVEEAKDKKEEVTHQDAIDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADASEAELSAAESSIQSSIIGLELRSNSNKGTVSETPVAKKANIVEAKEETN
                     PAATTDRSAVDSAVLPTSTAAKVETTSAPASINEILKPGLSLSDARQNPAIRKEDVDR
                     GYSGFRAAPAPTNQPVTNLGNNTVFTDISRGSHTFRGNGNSRRGNPIHFDVTTTRVRN
                     RVDFSIRYSGPGEFVNNNFILDKGNGFGEPSRATVSTPKLRDQVKNISKGGNFVSHSG
                     YSLEAAVETSTNQTIKFSLPIANPNGDLSVRLRPVTFNVGQGESGSATSNDPYSNSNY
                     YFREKPLLLDANPNGGTNNKTVSEDIDFQTVYLPTSKLPEGQTRLVREGEKGQRQIIY
                     KVHQLGNETILGLPISNSVIKEVKPRIMQIGVAKDLIDTVKPRVDQNKVGDTNNLTFY
                     LDNDGNGVYTEGVDELVQKIAIKDGAKGEKGDRGERGLTGAQGAKGEKGDRGERGLTG
                     AQGAKGEKGDRGERGLTGAQGAKGEKGDR"
BASE COUNT        11318 a         6899 c         8567 g        10885 t
ORIGIN      
        1 tttaattaaa ttgtaagtca atgcaagtca ataaggaatt ttcagataat tattgaaaaa
       61 gagacgtttt tatgttataa tgatagcgat taaattcgag gtgaaaaatg gcacatttat
      121 tagaaaaaac tagaaaaatt acatcaattt tgaaacgctc agaggagcag ttgcaggatg
      181 agctccctta caacgctatt acgcgtcagt tagcggatat tattcattgc aatgcctgca
      241 ttatcaatag taagggacgt ctgcttggct attttatgcg ttataaaaca aatacagatc
      301 gcgtagagca attcttccaa actaagattt tcccagatga ctacgttcaa ggggctaata
      361 tgatttacga aacagaagca aacttgcctg ttgagcatga tatgagtatt ttccctgttg
      421 agagtagaga tgattttcca gatggcttga cgactattgc accgattcat gtatcgggga
      481 ttcgccttgg ttctttgatt atttggcgta atgataaaaa attcgaagat gaggacttgg
      541 ttcttgttga gattgccagt accgttgttg ggattcagct tcttaacttc caacgtgaag
      601 aagatgagaa aaatattcgt cgtcgtactg ctgtcaccat ggcggtcaat accctttctt
      661 actccgaact ccgtgctgtt tcagcaattt taggggaatt aaatggaaat gaagggaagt
      721 tgactgcgtc agtgattgca gatcgtatcg gaatcactcg ctctgtgatt gtcaatgctc
      781 ttcgtaaact tgagtctgcg gggattattg aaagtcgctc acttggaatg aagggaacct
      841 atcttaaggt cttgatttca gatatttttg aagaagtgaa gaaaagagat tactaatgac
      901 aaaggcttta atttcgattg attatacaga agattttgtt gctgatagtg ggaagctgac
      961 agcaggtgct ccagctcagg cgatttcgga tgccatcagc aaggtaactc gattagcttt
     1021 tgaacgagga gattatatct tctttactat tgatgctcat gaagaaaacg attttttcca
     1081 tccggaaagt aagttatttc ctcctcataa tctgattggg acgagtggac ggaatttata
     1141 tggagatttg gggatctttt atcaagagca tggttcagac agtcgtgtct tttggatgga
     1201 taaacgccat tactcagctt tttcagggac tgacctagat attcgtttga gagagcgtag
     1261 agtgtctacc gttatcttaa caggtgtctt gacggatatc tgtgtcctac atacagctat
     1321 agatgcctat aatctaggat atgacatcga gattgttaaa ccagctgttg cttccatctg
     1381 gcctgaaaat catcaatttg ccctaggtca tttcaaaaat acactcggag ctaagttagt
     1441 agatgaaaat ctaaatgaac tttctgagta acctggttgg tgaaaatgaa aaaatttgaa
     1501 aaaaagtgtt gacaagcatc ccaaaagttg atatactagt aaagtaatcg atgcggggat
     1561 ggcggaattg gcagacgcgc aggactaagg atcctgtgac cgctttaggt cgtgagggtt
     1621 caagtccctc tctccgcata ggaaaagagt tagctaaagc tagctctttt gtatttctct
     1681 aaaaaattag aatccagtca aatgtagtgg agaatcagcg aaaatatggg aaaactgttc
     1741 cttgtttctg cttttgtcat agaagaataa taaagattat cttcttcaaa ttctccgata
     1801 ttctctaaag ttttgtgcaa gttgcacaga acttttttat ttttttggtc atcttgccat
     1861 agaaatataa agcgttttca tctataatat aattatcaaa agacaaaagg agttcacctc
     1921 atggtagaat tgaatcttaa aaatatttac aaaaaatatc caaacagcga acactattca
     1981 gttgaagatt tcaacttgaa catcaaagat aaagaattta tcgttttcgt aggaccttca
     2041 ggatgtggta aatcaactac actccgtatg attgctggtc ttgaagacat tacagaaggt
     2101 actgcatcta tcgatggcgt ggttgtcaac gacgtagctc caaaagaccg tgatatcgcc
     2161 atggtattcc aaaactacgc tctttaccca cacatgactg tttatgacaa catggctttc
     2221 ggtttgaaat tgcgtaaata cagcaaagaa gacattaaca aacgtgttca agaagcagct
     2281 gaaatacttg gattgaaaga attcttggaa cgtaaaccag ctgacctttc aggtggtcaa
     2341 cgtcaacgtg ttgccatggg gcgtgcgatt gtccgtgatg cgaaagtatt cttgatggac
     2401 gaacctttgt caaacttgga tgccaaactt cgtgtatcaa tgcgtgctga aatcgctaaa
     2461 attcaccgtc gtatcggagc tacaactatc tatgtaactc acgaccaaac agaagcgatg
     2521 acacttgcag accgtatcgt tattatgtca gctactaaga accctgctgg tacaggtact
     2581 atcggacgtg tagaacaaat cggtactcct caagaagttt acaaaaatcc agttaacaaa
     2641 ttcgttgcag gattcatcgg aagcccagct atgaacttca tcaacgtgaa attggttggt
     2701 agcgaaattg tttctgacgg tttccgtttg aaagtgccag aaggagcatt gaaagttctt
     2761 cgtgaaaaag gctacgaagg aaaagaattg atctttggta tccgtccaga agacgtgaat
     2821 gcagaacctg ctttccttga aacattccca gactgtgttg taaaagcgac tatctctgta
     2881 tcagaactgc ttggttcaga atctcacctt tactgccaag ttggtaaaga cgagtttgtt
     2941 gcaaaagttg atgctcgtga ctacttgcaa acaggtgcaa cagttgagct tggatttgac
     3001 ttgaacaaag cacacttctt cgatgtagaa actgaaaaaa caatctacta aaataaataa
     3061 aattcaaagc actacaagaa aagatatctc tttatcaatt gtagtggaga gatatcagtt
     3121 aatctaggga gagaaacaaa atgcttctct cctttttgct agggaagtca tattatgcat
     3181 ctatattgtg atgctcttta atactcttcg aaaatctctt caaaccacgt caacgtcgcc
     3241 ttgccgtacg tatgattact gatttcgtca gttttatctg caacctcaaa gatgtacttt
     3301 gagcagctta cggctagttt cctagtttgc tctttgattt ccattgagta ttatttgtgg
     3361 gtaccatcta caagtgaagc tatatgcgta aactacgtga gcaattgaat tcgaactaga
     3421 gaggtaataa taaatttatg ctatagttat ggtgacttgt atgcttttga ttctagttta
     3481 tcaaataata gattagaatt gtcagataat atcattttgt gttataatga agaaaaaaca
     3541 gaggtgttca aatgtcagaa gcaggtcata agtttttagc aaaattgggg aaaaaacgct
     3601 tacgtccagg tggaaagcgt gctacagatt ggttaattgc agaaggagga ttgtcaaaag
     3661 aaaagagaat actagaggtt gcgtgtaata ggggaactac agcaattgag ttggcacagc
     3721 gttttggttg caagataact gctgttgata tggatgctca agctttagaa gtggctaaaa
     3781 aatctgctgg aacggcaggt gttgctcatt taatcagttt tgaaagagca aatgcaatga
     3841 aacttcctta tcaagatgct agttttgata ttgttataaa tgaagctatg ctgactatgc
     3901 aagccgatca agctaagaaa aaatgtgtaa tggaatatct aagggtatta aaacctggag
     3961 gtcttctttt gacacatgat gtgcttctta aggaagctaa agagtctatc agacaggaat
     4021 tatcacaagc aattcatgta aatgtaggtc ctttaactca agatggttgg gaacaggtga
     4081 tgatagaatc aggttattgt gatgtgaaag tattgactgg tgaaatgaca ttaatgaaat
     4141 tatcgggtat gatttatgac gaaggttggc taggaacttt gaaaatttgt gtaaatgctt
     4201 gtaaaaagga gaatagaaag cagtttttaa ctatgtataa aatgtttgct aagaataaac
     4261 agaaattggg ctttattgcg atggctagtt ataaatcgtc aaaacgttag ataattattg
     4321 aagttaactt ttcctttttt ctttcttaaa aaatatgcta taatagagag tgaaaaactt
     4381 tgaaagaaag aaaaagatga atttaaaaga ttacattgca acaattgaaa attatccaaa
     4441 ggaaggcatt accttccgtg atattagtcc tttgatggct gatggaaatg cttatagcta
     4501 cgctgttcgt gaaatcgttc agtatgctac tgacaagaaa gtcgacatga tcgtgggacc
     4561 tgaagctcgt ggatttatcg tgggttgtcc agttgccttt gagttgggaa ttggttttgc
     4621 gcctgttcgt aagccaggta aattgccacg cgaagttatt tctgctgact atgaaaaaga
     4681 gtacggtgtc gataccttga ctatgcacgc ggatgccatt aagccaggtc aacgtgttct
     4741 tattgtagat gaccttttgg cgacaggtgg aactgttaag gcaactatcg agatgattga
     4801 aaaacttggt ggtgttatgg caggttgtgc cttccttgtt gaattggatg aattgaacgg
     4861 ccgtgaaaaa atcggtgact acgactacaa agttcttatg cattattaat gaaaacagtc
     4921 cctagggctg ttttctctac actaggatat aaaaatagac tataactagt tagagaaaaa
     4981 ctataattga aaactatatc ttcttgcagt ataataaaag gactaagtgt ttgagatttg
     5041 tcttcaaaca catgcaatta ttcctgagag agtacagtta ggagagggtt atgccgattc
     5101 gaattgataa aaaattgcca gctgttgaga ttttacggac agagaatatc tttgtcatgg
     5161 atgatcaacg tgctgcccac caagatatcc gtcctttgaa gattttaatt ttaaatctca
     5221 tgccacagaa aatggtcaca gagacccagt tgttgcgtca cttggctaat acacccctac
     5281 aactggatat tgattttctc tatatggaga gccaccgttc taaaacaact cgttcagagc
     5341 acatggagac cttctataaa acttttcctg aagtcaagga tgagtatttt gatgggatga
     5401 tcatcacggg tgctccagtt gagcatttac catttgagga agtggactat tgggaggaat
     5461 ttaggcagat gcttgagtgg tctaagacac atgtctattc gacccttcat atctgttggg
     5521 gggctcaggc tgggctttat ctgcgctatg gtgtagaaaa ataccagatg gacagtaagc
     5581 tatcaggtat ttatcctcag gacaccctaa aagagggtca ccttctattt agaggctttg
     5641 atgatagcta tgtatcccct cattcacggc acacggagat ttctaaggaa gaggtcttaa
     5701 acaagaccaa tctcgagatt ttatcagaag gacctcaggt tggggtttct attttggcca
     5761 gtcgtgattt acgagaaatt tatagttttg gtcatttgga gtatgaccgt gataccttgg
     5821 caaaagagta ttttcgagat cgtgatgcag gtttcgaccc acatattcca gaaaattact
     5881 ttaaagatga tgatgttaat caggtacctt gtctttgttg gtcttcatct gcagccctct
     5941 ttttcagtaa ttgggtaaac catgcggtct atcaggagac gccttttgat tggagaaaga
     6001 tagaagatga tgcatctgca tatgggtatt tataagagga attatgacat atttagacgc
     6061 ttttaaatca ggtaccttgg ttttaccgag tgccctgctc ttgcatttta aggaactctt
     6121 tccttctagc gacgattttc tggtttggca atttttctat ttgcaaaata cgacaggctt
     6181 agaagaaatg tcgccaagcc agattgctga aaggattggc aaggaaattt cggatgtcaa
     6241 ccagtccatt tctaatctga cggaaagggg actgctccag tatcgtacta tcgaattaaa
     6301 tggcgaaatt gaattgctct ttgatgctag tttggccttg gaacgtttgg atgacctgtt
     6361 tggagcagtt cgttcaagtt cagaccagct aacacctcaa aaccagctca aggatttggt
     6421 ggaaaccttc cagcaggagt tgggacgatt gttgacgcct tttgagattg aggatttgac
     6481 caagacacta aaggaagatg gaaccagtgc tgacttgatt aaggaggctc ttcgtgaagc
     6541 tgttttgaat ggaaaaccaa actggaagta cattcaggcg attttgagaa actggcgcca
     6601 tgaaggaatc aagagtgtgg ctcaaattga ggccaagaga gcagaaagag aagcaagcaa
     6661 tcctcagttg acacaggtat ctgcagattt cagaaatgcc atggatctct ggaaggatta
     6721 atccatgcaa gtaggcttga aatccgagta agatttgcaa gctgtgtata attgtgatag
     6781 aataaataga aaataaattg aaaaaagagg tatgtgaaat gtcacgtaaa ccatttatcg
     6841 ctggtaactg gaaaatgaac aaaaatccag aagaagctaa agcattcgtt gaagcagttg
     6901 catcaaaact tccttcatca gatcttgttg aagcaggtat cgctgctcca gctcttgatt
     6961 tgacaactgt tcttgctgtt gcaaaaggct caaaccttaa agttgctgct caaaactgct
     7021 actttgaaaa tgcaggtgct ttcactggtg aaactagccc acaagttttg aaagaaatcg
     7081 gtactgatta cgttgttatc ggtcactcag aacgccgtga ctacttccat gaaactgacg
     7141 aagatatcaa caaaaaagca aaagcaatct ttgcgaacgg tatgcttcca atcatctgtt
     7201 gtggtgaatc acttgaaact tacgaagctg gtaaagctgc tgaattcgta ggtgctcaag
     7261 tatctgctgc attggctgga ttgactgctg aacaagttgc tgcctcagtt atcgcttatg
     7321 agccaatctg ggctatcggt actggtaaat cagcttcaca agacgatgca caaaaaatgt
     7381 gtaaagttgt tcgtgacgtt gtagctgctg actttggtca agaagtcgca gacaaagttc
     7441 gtgttcaata cggtggttct gttaaacctg aaaatgttgc ttcatacatg gcttgcccag
     7501 acgttgacgg tgcccttgta ggtggtgcgt cacttgaagc tgaaagcttc ttggctttgc
     7561 ttgactttgt aaaataatca gtaagtagca aaagctaggt ggaacagcat tcagatgtct
     7621 gttacatttt ttataggaga ggaaagattg aaaacaaaaa ttggattagc aagtatctgt
     7681 ttactaggct tggcaactag tcatgtcgct gcaaatgaaa ctgaagtagc aaaaacttcg
     7741 caggatacaa cgacagcttc aagtagttca gagcaaaatc agtcttctaa taaaacgcaa
     7801 acgagcacag aagtacagac taatgctgct gcccactggg atggggatta ttatgtaaag
     7861 gatgatggct ctaaagctca aagtgaatgg atttttgaca actactataa ggcctggttt
     7921 tatattaatt cagatggtcg ttactcgcag aatgaatggc atggaaatta ctacctgaaa
     7981 tcaggtggat atatggccca aaacgagtgg atctatgaca gtaattacaa gagttggttt
     8041 tatctcaagt cagatggggc ttatgctcat caagaatggc aattgattgg aaataagtgg
     8101 tactacttca agaagtgggg ttacatggct aaaagccaat ggcaaggaag ttatttcttg
     8161 aatggtcaag gagctatgat gcaaaatgaa tggctctatg atccagccta ttctgcttat
     8221 ttttatctaa aatccgatgg aacttatgct aaccaagagt ggcaaaaagt gggcggaaaa
     8281 tggtactatt tcaagaagtg gggctatatg gctcggaatg agtggcaagg caactactat
     8341 ttgactggaa gtggtgctat ggcgactgac gaagtgatta tggatggtgc tcgctatatc
     8401 tttgcggcct ctggtgagct caaagaaaag aaagatttga atgtcggctg ggttcacaga
     8461 gatggtaaac gctatttctt taataataga gaagaacaag tgggaaccga acatgctaag
     8521 aaaatcattg atattagtga gcacaatggt cgtatcaatg attggaaaaa ggttattgat
     8581 gagaacgagg tggatggtgt cattgttcgt ctaggttata gcggtaaaga agacaaggaa
     8641 ttggcgcata acattaagga gttaaaccgt ctgggaattc cttatggtgt ctatctctat
     8701 acctatgctg aaaatgagac cgatgctgag agtgacgcta aacagaccat tgaacttata
     8761 aagaaataca atatgaacct gtcttaccct atctattatg atgttgagaa ttgggaatat
     8821 gtaaataaga gcaagagagc tccaagtgat acaggcactt gggttaaaat catcaacaag
     8881 tacatggaca cgatgaagca ggcgggttat caaaatgtgt atgtctatag ctatcgtagt
     8941 ttattacaga cgcgtttaaa acacccagat attttaaaac atgtaaactg ggtagcggcc
     9001 tatacgaatg ctttagaatg ggaaaaccct cattattcag gaaaaaaagg ttggcaatat
     9061 acctcttctg aatacatgaa aggaatccaa gggcgcgtag atgtcagcgt ttggtattaa
     9121 gcgatgattt gaaagaggga tgtgatagta gcaccctctt tttctttgtt ttatgatagt
     9181 tcatcctcga gtaaattcaa gttcttgctc ggaaatgaag cttatatagt agattgaata
     9241 tagacaaata ccttgtgatt ggtaaaacat tttagaaatt catttacctt tcctaatcga
     9301 cttggtttca tcttatttca atctattata gtattgggga atttcttcaa accacatcag
     9361 cttggtcagt tctacctgcg acctcaaaac ttgtgctttg gtcaagctgg gtttagtttc
     9421 ctagtttgct gatggatttc cattgactat aagcatccaa ccctcttttt gtcttctaaa
     9481 gaattcttaa attatcagtc tattgcaact tttctcatat aagttctttg tcttgctatt
     9541 ggttttcctt agtagtatac taaggtagta atcattaaga agtggttaca aaaaataatg
     9601 aatgaggtaa agaaaatggt agaattgaaa aaagaagcag taaaagacgt aacatcattg
     9661 acaaaagcag cgccagtagc attggcaaaa acaaaggaag tcttgaacca agctgttgct
     9721 gatttgtatg tagctcacgt tgctttgcac caagtgcact ggtatatgca tggtcgtggt
     9781 ttccttgtat ggcatccaaa aatggatgag tacatggaag ctcttgacgg tcaattggat
     9841 gaaatcagtg aacgcttgat tacactcggt ggaagcccat tctctacatt gacagagttc
     9901 cttcaaaata gtgaaatcga agaagaagct ggtgaatacc gtaatgttga agaaagcttg
     9961 gaacgtgttc ttgttatcta ccgttacttg tcagaacttt tccaaaaagg tttggatgtc
    10021 actgatgaag aaggtgacga tgtgacaaac ggtatctttg caggcgctaa aactgaaaca
    10081 gataaaacaa tttggatgct tgcagccgaa cttggacaag cacctggttt gtaagaaata
    10141 aggaaacata gataaatctg taggaaattt cttacagatt ttttctattt tgtaagctat
    10201 tccaaaccag tctttttttg agttttttga taaaatagta ctatcagtga aaaggatgga
    10261 agcatgacta agaaaatcgt agctatttgg gcccaggatg aagagggtgt gattggtaag
    10321 gaaaatcgtc tgccttggca tttgccagca gaattgcagc actttaaaga aacaactctg
    10381 aatcatgcta tcttgatggg gcgtgtgacc tttgatggga tggggcgtcg cttgcttcca
    10441 aaacgggaaa ccttgatttt gacgcgtaat ccggaagaaa agatagatgg ggttgctact
    10501 tttcaggacg tccagtctgt tcttgactgg tatcaggctc aagaaaagaa tctctacatt
    10561 atcggtggga agcaaatttt tcaggctttt gaaccttacc ttgatgaagt gattgtcact
    10621 cacattcatg ctcgggtgga aggagatacc tatttccctg aagaacttga cttgtctctt
    10681 tttgagacag tttcaagcaa attttacgcc aaagatgaga agaatcctta tgattttacc
    10741 atccaatacc gcaagagaaa ggaagtctaa tggaacggag tttatttggt ctattcacgg
    10801 cttttctgtg tttcatctgc tttctggcag gagcacaagc ttttcgtaaa aagcgttacg
    10861 ggctttctat cctcctttgg ttaaatgcct ttaccaatct ggtcaatagt attcatgctt
    10921 tttacatgac cttattttag atagaatgac agtatagaat ggaaggaaat catgtctaca
    10981 aatagaaaaa atgatatgat ggtttattgc tcattttgtg gcaaaaacca agaagaagta
    11041 caaaaaataa ttgctggcaa caatgctttt atttgtaatg aatgcgtgga gttagctcag
    11101 gaaatcattc gagaagaatt ggttgaggaa gtcttggcag acttgtctga ggtgccaaaa
    11161 ccaattgaac tcctccatat cttgaaccac tatgtaattg gtcaagatcg tgccaagcgt
    11221 gccttggcag tggcggttta taaccactac aaacgcatca atttccacga tacacgcgaa
    11281 gagtcagaag atgtggattt gcagaagtca aacattttga tgattggccc aactggttca
    11341 gggaaaactt tccttgccca gaccttggct aagagcttga atgtaccttt tgctattgcg
    11401 gatgcgacag ctctgacgga ggctggttat gtgggtgagg atgtggaaaa tatactcctc
    11461 aaactcttgc aggttgctga ctttaacatc gaacgtgcag agcgtggcat tatctatgtg
    11521 gatgaaattg acaagattgc caagaagagt gagaatgtgt ctatcacacg tgatgtttct
    11581 ggtgaagggg tgcaacaagc ccttctcaag attattgagg gaactgttgc tagcgtaccg
    11641 cctcaaggtg gacgcaaaca tccacaacaa gagatgattc aagtggatac aaaaaatatc
    11701 ctcttcatcg tgggtggtgc ttttgatggt attgaagaaa ttgtcaaaca acgtctgggt
    11761 gaaaaagtca tcggatttgg tcaaaacaat aaggcgattg acgaaaacag ctcatacatg
    11821 caagaaatca tcgctgaaga cattcaaaaa tttggtatta tccctgagtt gattggacgc
    11881 ttgcctgttt ttgcggctct tgagcaattg accgttgatg acttggttcg catcttgaaa
    11941 gagccaagaa atgccttggt gaaacaatac caaaccttgc tttcttatga tgatgttgag
    12001 ttggaatttg acgacgaagc ccttcaagag attgctaata aagcaatcga acggaagaca
    12061 ggggcgcgtg gacttcgctc catcatcgaa gaaaccatgc tagatgttat gtttgaggtg
    12121 ccgagtcagg aaaatgtgaa attggttcgt atcactaaag aaactgtcga tggaacggat
    12181 aaaccgatcc tagaaacagc ctagaggtga ctatggaact taatacacac aatgctgaaa
    12241 tcttgctcag tgcagctaat aagtcccact atccgcagga tgaactgcca gagattgccc
    12301 tagcagggcg ttcaaatgtt ggtaaatcca gctttatcaa cactatgttg aaccgtaaga
    12361 atctcgcccg tacatcagga aaacctggta aaacccagct cctgaacttt tttaacattg
    12421 atgacaagat gcgctttgtg gatgtgcctg gttatggcta tgctcgtgtt tctaaaaagg
    12481 aacgtgaaaa gtgggggtgc atgattgagg agtacttaac gactcgggaa aatctccgtg
    12541 cggttgtcag tctagttgac cttcgtcatg acccgtcagc agatgatgtg cagatgtacg
    12601 aatttctcaa gtattatgag attccagtca tcattgtggc gaccaaggcg gacaagattc
    12661 ctcgtggtaa atggaacaag catgaatcag caatcaaaaa gaaattaaac tttgacccaa
    12721 gtgacgattt catcctcttt tcatctgtca gcaaggcagg gatggatgag gcttgggatg
    12781 caatcttaga aaaattgtga ggaaaagaaa atggcaaaaa caattcatac agataaggcc
    12841 ccaaaggcta tcggacccta tgttcaagga aaaatcgttg gcaacctttt gtttgctagc
    12901 ggtcaagttc ccctatcccc tgaaactggg gaaattgtag gagagaatat ccaagaacag
    12961 acagagcaag tcttgaaaaa catcggtgct attttggcag aagcaggaac agactttgac
    13021 catgttgtca aaacaacttg cttcttgagc gatatgaacg actttgttcc ttttaatgag
    13081 gtttaccaaa cggccttcaa agaggaattc ccagctcgtt cagctgtgga ggtagctcgt
    13141 cttcctcgtg atgtaaaagt cgaaattgaa gtcatcgcag agattggata agctagttga
    13201 agtttggtgt tgccaaactt cttttgatat aaggagaaaa agatgacaaa gaaacaactt
    13261 cacttggtga ttgtgacagg gatgagtggc gcagggaaaa ctgtagccat tcagtccttc
    13321 gaggatctag gttatttcac cattgataat atgccgccag ctctcttgcc taagtttttg
    13381 cagctggttg aaattaagga agataatcct aagttggctt tggtagtgga tatgcgtagc
    13441 cgttctttct tttcagagat tcaagctgtt ttggatgagt tggaaaatca agatggtttg
    13501 gatttcaaaa tcctcttttt ggatgcggct gataaggaat tggtcgctcg ttacaaggaa
    13561 accagacgga gtcacccact agcagcagac ggtcgtattt tagatggaat caagttggaa
    13621 cgtgaactct tggcaccttt gaaaaatatg agccaaaatg tggtggatac gactgaactc
    13681 actccacgtg aactgcgcaa aacccttgca gagcagtttt cagaccaaga acaagcccag
    13741 tctttccgta tcgaagtcat gtctttcgga tttaagtatg gaatcccgat tgatgcggac
    13801 ttggtctttg atgtccgttt cttgccaaat ccctattatc taccagaact gagaaaccaa
    13861 acgggtgtgg atgaacctgt ttatgattat gtcatgaacc atcctgagtc agaagacttt
    13921 tatcaacatt tattggcctt gattgagccg attctgccaa gttaccaaaa ggaaggtaag
    13981 tccgttttga ccattgctat gggatgtacg ggtggacaac accgtagtgt ggcatttgct
    14041 aaacgcttgg cgcaggactt atccaagaat tggtctgtta atgaagggca tcgcgacaaa
    14101 gaccgcagaa aggaaacggt aaaccgttca tgagaaaacc aaagataacg gtgattggtg
    14161 gagggactgg aattcccgtc attctaaaaa gtctgcggga aaaagatgtg gaaatcgcag
    14221 ctatcgtgac ggtggcagat gatggtggtt cttcaggtga actccgaaaa aatatgcaac
    14281 agttgacacc gccaggtgat cttcgtaatg tccttgtggc catgtcggat atgcctaagt
    14341 tttatgagaa ggtctttcag tatcggttct ctgaggatgc cggagccttt gctggccatc
    14401 cattgggaaa tctcatcatt gctggcttgt cagaaatgca gggttcaacc tataatgcca
    14461 tgcagttatt gagcaaattt ttccatacaa cagggaaaat ttatccttcc agtgaccatc
    14521 ctttgaccct tcatgcagtc tttcaggatg ggacagaagt ggctggagag agtcatattg
    14581 tagaccatcg aggcataatt gacaatgtct atgtgaccaa tgccctaaac gatgatacgc
    14641 ctctggccag ccgtcgagta gtgcagacca tccttgaaag tgacatgatt gtcctagggc
    14701 caggttccct ctttacctct attttgccca atatcgtgat taaggaaatt gggcgggctc
    14761 ttttggaaac taaggcagaa attgcctatg tctgcaatat catgacccaa cgtggggaga
    14821 cggaacactt tacagatagc gaccacgtgg aagtcttgca tcgtcacctt ggtcgccctt
    14881 ttatcgacac tgtcttggtg aatattgaaa aagtgcctca ggaatacatg aattccaacc
    14941 gttttgatga atacttggtg caagtggaac acgattttgt aggtctttgt aagcaagttt
    15001 cgcgcgtgat ttcatctaac ttccttcgtc tggaaaatgg cggtgccttc cacgatggag
    15061 atttgattgt ggacgagttg atgcgcatta tacaggtgaa aaaatgagtt tcacagtagc
    15121 agtaaaagaa gaaatcctgg gccaacacca tctgagccgg catgaattat ctgccattat
    15181 caaaatgtct ggtagcatcg gtctctcgac ttcgggcttg actttgtcag ttgtgacaga
    15241 aaatgccaaa ctggcccgtc acctctatga gtcctttctc catttctatg aaatcaaatc
    15301 tgaaattcgt caccaccaac ggagcaatct tcgcaagaat cgcgtctata ccgtttttac
    15361 agatgaaaag gtgcaggatt tgttaagtga tttgcacttg gcagactctt tctttggcct
    15421 ggaaacaggt attgatgagg cgattttatc ggatgaggaa gcaggtcgtg cctatctctg
    15481 tggcgctttc ttggtaaatg ggagcattcg tgaccctgag tcaggcaagt accagttgga
    15541 aatcagttct gtttatctgg accacgcgca aggaattgcc tcccttctcc aacagttttt
    15601 actggatgcc aaggtgcttg agcgcaagaa gggggctgtg acctatctcc agcgagcaga
    15661 agacattatg gacttcttga tagtaatcgg agccatgcag gcacgtgatg attttgagcg
    15721 ggttaagatt ttgcgagaaa cccgtaacga cctcaatcgg gctaataatg ccgagacagc
    15781 taatatcgct cggacagttt ctgccagcat gaagaccatt aacaatatca gtaaaataaa
    15841 agatatcatg ggcttagaaa atttgccagt agatttgcag gaagtggcgc aactgaggat
    15901 tcagcaccca gactactcta tccagcagtt agcagatagt ctcagcaccc ctctgaccaa
    15961 aagtggtgtc aatcacagac tcagaaaaat caataagata gcggatgaat tataaaacca
    16021 cgaatcctat gtgactcgtg gttctttttt ataaactggt agagtgtttt ggttgtactt
    16081 tttgttcagg gtcaatgtag ttgatagcgt tgttgacagc agttggagct tctccgaggc
    16141 ctgtcgcaat cagatcaatt tttccgtcat agtagcagca gtcaccgata gcatagatac
    16201 ctgcttggct ggattcctgt ttgctgttga cgataatctt gtgacggttg aggtcgagcc
    16261 cccagttttt aaggttaccg acagaagatt tgaaaccata gttgacaaag aggtggtcta
    16321 ggtcaatggt ttcagtttca tcagatttga cttttgtgat ttcaagttta tcaagtgttt
    16381 ttccatttcc aaggagttgg ctaggggcga atggtgtctt gatggttaca gatgattctt
    16441 gcaaggcttg aacactgtgt tccaaggcac ggaaattatc tctgcggtga acaagggtag
    16501 ttggtgcgat tttttcaaaa gccaaagtcc aatccacagc cgagtctccc ccaccaagaa
    16561 tcgtcacttt tttaccagcg tattgctgaa tgttagaaac gtggtagtgg atattttcat
    16621 agccctcaac cccttcaagt tccagcggac gtggtttgaa ggcaccgcca cccatagcga
    16681 tgataactgt tttagtcagg tgacttcctt tagaagttgt gatggcaaat tcttcttctt
    16741 gtttgtcaat ctcaagaacc gtttcattga gatgaatagg ggtatcaaat ccatttagct
    16801 gttcaatcaa gcggttagtc aactcttctc cagtcaggtt tgggaagcct ggtacgtcta
    16861 ggatttcctt ttcagggtag agaatagcag gttgtccacc tagctgggga agagagtcga
    16921 tgatttgaac cttggcttgg cgtaggtggg cataaaaggc tgcaaaaagc ccgacaggac
    16981 caccacccac aatggtaata tcatagagtt gagacatagt ttctcctttg ttttttctag
    17041 tcagtttatt ttatcatatt tttgttcaat gtaaaatgga ctaggatagg gaaaaaaatg
    17101 ccagaaaatg gtataatagg acgaaagtgt tttgataggg aggatatcag ggatgaattt
    17161 tcaacaatta tccaatttgc aatattggac cagtttgttt gcaagtccat ggacgatagc
    17221 tatcaatctg attgatattt tgattgttgc ttatatttta taccatttta caaaagctat
    17281 tgcaggaacc aagattatga ttttggtacg tggagttttg gtatttattt tagctcaaat
    17341 ccttgcaaat atgattggtt tgactacgat ttcttggtta atcaatcaaa ttattactta
    17401 tggggttatt gcggcggttg ttatcttctc tccagagatt cggactggtt tggaacgttt
    17461 gggaagagcg acagatttct tttccaatgc ccctattagt gctgaggaac agatgattcg
    17521 tgcctttgtt aagtctgttg aatacatgag tcctcgtaaa atcggggcct tggttgctat
    17581 tcagcgtgta cgtaccttgc aggagtatat ttcgacagga attcccttgg atgctaagat
    17641 ttctgcagaa cttctcatta acatttttat tcccaacact cccctacatg acggtgcggt
    17701 gattatcaaa gaagaacgta tcgctgtgac gtctgcctat ctgcccttga caaaaaacac
    17761 agggatttcc aaggaatttg ggaccagaca ccgggcggct atcggtttat cagaagtctc
    17821 agatgccttg acttttgtcg tatcagagga aacgggagga atttcgataa cctataatgg
    17881 aaggtttaag cacaacctaa cacttgatga atttgaaaca gaattacgtg aaatcttact
    17941 tccaaaagag gaagtaggtc ttagttttaa agaacgattg ctaggaggat ggaaacatga
    18001 aaaaaaatag tttatatatc atatcctcac tcttttttgc ttgtgtctta tttgtctatg
    18061 ctacggcgac gaattttcaa aacagtacca gtgctaggca ggtaaaaacg gaaacctata
    18121 ctaatacagt aacaaatgtc cctattgaca tacgctataa tagtgataag tattttatta
    18181 gcggttttgc ttcagaagta tcagtggtct tgactggtgc aaatcgccta tcgctagcta
    18241 gtgaaatgca agaaagtaca cgtaaattca aggttactgc tgacctaaca gatgccggtg
    18301 ttggaacaat tgaagttcct ttgagcattg aagatttacc caatgggctg accgctgtgg
    18361 cgactccgca aaaaattaca gtcaagattg gtaagaaggc tcagaaggat aaggtaaaga
    18421 ttgtaccaga gattgaccct agtcaaattg atagtcgggt acaaattgaa aatgtcatgg
    18481 tgtcagataa agaagtgtcg attacgagtg accaagagac attggataga attgataaga
    18541 ttatcgctgt cttgccaact agcgaacgca taacaggtaa ttacagtggt tcagtacctt
    18601 tgcaggcaat cgaccgcaat ggtgttgtct taccggcagt tatcactccg tttgatacaa
    18661 taatgaaggt gactacaaaa ccagtagcac caagttcaag cacatcaaat tcaagtacaa
    18721 gcagttcatc ggagacatct tcgtcaacga aagcaactag ttcaaaaacg aattaaaaat
    18781 agaaaaggat tttataacaa tgggtaaata ttttgggact gatggagtcc gtggagaagc
    18841 taacctagaa ctaacaccag aattagcctt taaactagga cgttttggag gctatgttct
    18901 tagtcaacat gaaacggaag cgccgaaagt ctttgtagga cgtgacacac gtatttcagg
    18961 ggaaatgcta gaatcggcct tggtggcagg tctcctttca gtagggattc acgtatacaa
    19021 acttggtgtc cttgcaacac cagcagtagc ttacttggtt gaaactgaag gagcaagtgc
    19081 tggtgtcatg atttctgcta gccacaatcc agctcttgat aacggaatca agttctttgg
    19141 cggtgatggc ttcaaactag atgatgaaaa agaagcagaa attgaagcct tgctagatgc
    19201 tgaggaagac actcttcctc gtccaagtgc agaaggctta ggaattttgg tagattatcc
    19261 agaaggcttg cgtaagtatg aaggatacct tgtttcaact ggaactcctc ttgatggaat
    19321 gaaggttgcc ttggatacag ctaatggagc agcttctacc agtgcccgtc aaatctttgc
    19381 agaccttggt gcccaattga cggttatcgg ggaaacacca gacggtctta acatcaacct
    19441 taatgttggt tcaacacatc cagaagccct tcaagaagtg gtcaaagaaa gtgggtcagc
    19501 tattggtttg gcctttgatg gagacagtga ccgcttgatt gctgttgatg agaatggtga
    19561 catcgtcgat ggtgacaaga ttatgtacat catcggaaaa tacctttctg aaaaaggaca
    19621 attggctcaa aatacaattg tgacaactgt tatgtctaac cttggtttcc acaaggcctt
    19681 gaatcgcgaa ggtattaaca aggcagttac tgcagttggt gaccgctacg ttgttgaaga
    19741 aatgagaaaa tcaggttaca accttggtgg tgaacagtct ggtcacgtta tcttgatgga
    19801 ttacaatacc acaggtgatg gtcaattatc agcaattcaa ttgactaaaa tcatgaagga
    19861 aactggtaag agcttatcag agttggcggc agaagtaacg atttatccac aaaaattagt
    19921 taatatccga gtggaaaacg tcatgaagga aaaggccatg gaagtgccag ctatcaaggc
    19981 catcatcgag aagatggaag aagaaatggc ggggaacggc cgtatccttg ttcgtccaag
    20041 tggaacagag cccctcttgc gtgttatggc agaagcgcct acaacagaag aagtggacta
    20101 ctatgttgat actatcacag atgtagttcg tgctgaaatt gggattgact aaatcctaga
    20161 aaactagatg aaaataaaaa attatggtac aatagcttat aatattgtag ccgatggaga
    20221 aagacatgaa tcttaaacgt gaacaagaat ttgttagtca gtatcatttt gatgcacgta
    20281 attttgaatg ggaaaatgaa aatggagctc ctgaaaccaa ggtggatgtg aatttccaat
    20341 tgctccaaca tgaccaagaa aatcaagtga cttccttgat tgtcattttg agctttatga
    20401 ttgtatttga taagtttgtc attagtggaa caatctcaca agtaaaccat atcgatggcc
    20461 gtattgtcaa tgaaccaagt gaattgaacc aagaagaggt ggaaaccttg gctcgtccat
    20521 gtttgaacat gctcaatcgt ttgacttacg aagtgactga aattgcatta gacttaccag
    20581 gaattaattt ggagttctaa tatgaaatta gctgttttca cagattcctc tgcttatctc
    20641 agtgaagaga ccttgcaaag agaagacttg tttgtcttgg atattcctgt caatattgat
    20701 ggtgaggaat atgtcgaagg catcaatctg tctgctgagg aattttacca aaaaatggct
    20761 caggcttctg aattgcctaa gaccagtcaa ccaagtattg ccaagttaga tgagatctta
    20821 acttcgctca aagaacaagg ctatacacat gccttggggc ttttcctatc ttctggaatt
    20881 tcaggttttt accaaaatat ccagtatatg gtcgatgact atgagggctt aaccattgct
    20941 ttcccagaca ctttgattac aagtgctccc ctaggcatca tggttgaaag cgtctttaat
    21001 tggcgtgatc agggcgatga ttttgccagc attcaggata agctagccat tcaaatcagc
    21061 cgtacgtcag cctttatcat ggtagatgac ttggatcatt tggtaaaagg tggacgcctt
    21121 tcaaatgggg ctgccatttt gggcaatttg cttagcatta agccaatcct ttattttaac
    21181 gatcaaggtg tgattgaagt ttacgaaaaa gttcgtactg aaaagaaggc caccaagcgc
    21241 ttaattgaaa ttatcaagga aacaacggct tcaggtcaat accgggtcat tgtcattcat
    21301 gggaatgctc ctgaaaaggc tgaagaattg cgtcagcact tgcttgattt tggcttgggt
    21361 tcggatgttt cacttgctac atttggtagt gtcattggaa cgcacctagg agcaggaagt
    21421 attgctctgg gttatattcc agtgatttag ttggcgcttc taggctagtt aagatgcctt
    21481 gtatcttaga aattgaacac ggactacctg cctcttgaaa aaagatgcct gtcttgcctt
    21541 tcgtggaaag tcagtgccat tccctatttt tcagaggcag ctaaggtcct ttgtatcttg
    21601 ataattgaac acgcctggaa ccctgtgtga aaaagatagt tcttccaaga agtagacact
    21661 tcttgattgg aattcctatt ttcactttgt gttcttacgg actttgtatc ttagacagga
    21721 gtagagatga gtattcgagt aattattgcc ggttttaagg gaaagatggg ccaggctgct
    21781 tgtcagatgg tattgactga tccagacttg gacttggtgg cagttttgga tccttttgag
    21841 tctgagtcag aatggcaggg tattcctgtt ttcaaggata aggctgattt agctggtttt
    21901 gaagcggatg tctgggtaga ttttactact ccagctgttg cctacgaaaa tacacgtttt
    21961 gctcttgaaa atggctttgc tccagtagtt ggaacgactg gtttcacgag tgaagaaatt
    22021 gcagagctaa aagaattttc tcgtgcccaa gacttgggtg gcctgattgc ccctaacttt
    22081 gccttgggtg ctgtcttact catgcaattt gcgacgcagg ctgccaaata tttcccaaat
    22141 gtggagatta ttgagctcca tcatgacaag aaaaaggatg ctccgagtgg aacagccatt
    22201 aaaacagctg agttgatggc agaggttcga gagtccattc agcaaggcgc agcagatgag
    22261 gaagagctga ttgctggtgc tcgtggtgct gactttgatg atatgcgcat ccactcagtt
    22321 cgtttgccag gcttggtagc tcatcaggaa gtcatctttg gcaatcaggg agaagggttg
    22381 accctccgtc atgactccta tgatcgcatc tccttcatga caggagtcaa tttgggaatt
    22441 aaagaagttg tcaagcgtca tgagcttgtc tatggattag aacacttatt atgagattaa
    22501 cgcaaatgcc ttctgaattt cagaaggctt taccagtatt agaaaaaatt aaagaagcag
    22561 gctttgaggc ttattttgtt gggggctctg ttcgagatgc ccttctccat agccctatcc
    22621 acgatgtgga tattgcgacg tcttcttatc cagaagagac caagcagatt tttccgcgaa
    22681 cagccgatat cggaatcgag catggaaccg tcttggtctt agatggggat gaggagtatg
    22741 aggtaacaac ctttcggaca gaggatgtct atgtggacta tcgcagaccc agtgcggttt
    22801 cctttgtacg atcgctagaa gaagacctca aacgccgtga tttcacagtc aacgcctttg
    22861 ccttggatga gacaggagaa atcgttgact tgttccatgg tttagaagat ttggaaaagc
    22921 aagtcttgcg agcagttgga gtggctagtg agcgtttcaa cgaagatgct ttacggatta
    22981 tgcgtggttt ccgttttcag gctagtcttg gttttgcact tgagccagaa acatttaaag
    23041 ctatgaagac cttgacgccg cttttggaga aaatttctgt agagcgtacc ttcgtcgagt
    23101 ttgataaact cttgctggct ccattttgga gaaggggctt ggcttccatg attgagagtc
    23161 aagcttatga ctatctccct gatatggcat ctagccagga caagctcaac agactgtttg
    23221 atttggagac tgattttact tttgaatcct ctgaacaagc ctgggcggct ttactatggg
    23281 ctttggagat tgaaaatgcg cagtcatttt tgaaatcttg gaagacctca cgccagtttg
    23341 ccaagcaagt tcaggatttg ctgattattt tggctctgcg tgaaaatgga gaattgagta
    23401 agcgagattg ttatcgcttt gacatagatt tgcttttaca ggctgaaaat cttcgtcaag
    23461 ctcaaggaaa agaagtcaac ccacaagcca tcacagaaaa ataccaaagc ttgactatcc
    23521 atgacaagaa agagattcag attaatggcg atattttgat caaggaatat ggctatcagc
    23581 caggcccaga cttgggagag attttaacag agattgagtt tgccattgtc gatggagaat
    23641 tggagaataa tcgtgaagcc atccatgctt acctgaggga gaaaaaatga gtgattttat
    23701 cgttgaaaaa ctaagtaaat ccgttggtga caagaccgtt tttagggata tttcctttat
    23761 tatccatgac ttagacagaa ttggtttaat cggtgtcaat gggactggca agaccaccct
    23821 tttggacgtc ctttctggtg tttctggatt tgatggggat gtcagtcctt tttcagctaa
    23881 aaatgattac cagattggtt acttgactca ggatcctgat tttgatgata gaaagacagt
    23941 tttggatacg gttctatcta gtgaactcaa ggaaatccag ctcattcgtg agtatgaatt
    24001 gattatgctc gactatagtg aggacaagca ggcgcgtttg gaacgtgtca tggcagagat
    24061 ggactctctc caagcttggg aaatcgaaag tcaggtcaag accgttctta gcaaattggg
    24121 cattcaagac ttatctactc ctgttgggga attgtcaggt ggtctgagaa gacgggtaca
    24181 gttggcacaa gtcttacttg gcaaccacga cctcttgctt ttggatgagc cgaccaacca
    24241 tctggatatt gcgattattg agtggctgac cctctttttg aaaaattcta agaagaccgt
    24301 cctttttatc actcacgatc gttatttctt agacgctttg tcaacacgga ttttcgagtt
    24361 ggatcgtgca ggcttgaccg agtaccaggg caattaccag gactatgttc gcctaaaggc
    24421 ggaacaggat gagcgcgacg cggctcttct tcacaaaaaa gaacaactct acaaacaaga
    24481 actggcctgg atgcgcagac aaccgcaggc gcgtgcgacc aagcagcagg cccgtatcaa
    24541 tcgattccat gatctgaaaa aggaagtttc aggcagtagt gctgagacag acttgaccat
    24601 gaactttgaa accagtcgga ttgggaagaa agtcatcgag tttcaggctg tttcctttgc
    24661 ctatgaaaac aagcctattt tgcaagattt taatctcttg gtgcaggcca aagaccgtat
    24721 cggaatcgtt ggggacaatg gtgttggaaa atcaaccctg ctcaacttga ttgcaggaag
    24781 tcttgagcca actaaaggac aagttgtgat tggggaaact gttcgcatcg cctatttctc
    24841 tcagcaaatt gagggtttgg atgaaagcaa gagagttatc aattacctgc aggaagtggc
    24901 agaagaggtc aagaccagtg gcggttctac gacttcgatt gcagagttgc tggagcagtt
    24961 ccttttccca cgttcgacgc atggaacctt gattgagaaa ttgtcagggg gtgagaaaaa
    25021 acgtctttat ctcctcaaac tgcttttgga aaaaccaaat gttcttcttt tagacgagcc
    25081 aaccaatgac ctagatattg caactttgac agtcttagag aatttcttgc aaggttttgc
    25141 aggtcccgtt ttaacagtca gtcacgaccg ctatttcttg gataaggtag cgaccaagat
    25201 tctcgctttt gaggatagca agattcgtcc tttctttggt cattacaccg actatcttga
    25261 tgaaaaagct tttgaaacag atatggccaa tcaagtgcaa aaggtcgaaa aggaaaaagt
    25321 ggtcaaggtt cgagaagaca agaaacgtat gacctaccaa gaaaagcagg agtgggcaag
    25381 tattgaaggt gatattgaaa ccttggaaaa acgtatcgct gctattgaag aggaaatgca
    25441 ggctaacggc tctgactttg gtaagctggc tactctccaa aaagaattgg atgagaaaaa
    25501 tgaagcactc cttgaaaaat acgaacgcta tgagtatctc agtgaatttg atagttaaga
    25561 ctattcgaaa atcaaagtgc aaactatgtc ggcttcacat tgccgactca agtacagcct
    25621 gtgaccggct tcctaatttg cactttgatt tttattgagt ataaaagaat agaaaaatcc
    25681 cgtctcatga aaggatttct ctattctttt ttggtttctt gatgaaagat attttgccag
    25741 gtttcgtggc gacgccagat actggtatgg atgataccat ctaactcata gcagatgagt
    25801 ttggtttttt gactgatgga agtaatttga atgtccttga tagcagaatt taactctttt
    25861 ttggtcttat aagcctcttt atccatctgc tctccatctt ggcgaatata gacaaaatca
    25921 tcagtcaaga gttcttctag ttggtttcct tggtcaatca attgttcacg cataagcaat
    25981 tttttataga cattgtctaa aatttcatcc tcagagatag gtgctggttc gatatggaca
    26041 tcggtatcaa agacgccaaa acgctcctcc agcatagact cgacctgatc cgcgatttca
    26101 tggctttcaa aaacagacaa gtcaggattc atctctagtg taatatccag gtagatgttg
    26161 ctaccgtagg tgcgacctct ttgcgatttg accttgctga ttttgggaat ttccatgata
    26221 gccttttggt agtcctcgag caggcggtcg tcaaagccat ctgaaagact aaaggaagac
    26281 tcgatgaaga tatcataggc agtcttcaag ataaagaaag tgatgatgat agcaaccagt
    26341 ttatccacaa tcggataatt gaaactacta gctaggatgg caatggcggt tccaagtgag
    26401 gtaacagcgt cagaaagatt gtccttagca gctgccttca gcgccttgga gttggatttc
    26461 ttactgaggc gagtattgta gagatagacc acaaacataa tcgctgcaga aatgattcct
    26521 agagttgcac caagaggatc aatgaccgtt tcttcccgac tgagaatctt ttgaatggta
    26581 tctcttagaa catcgaaacc gacatagaac atgatgatag aagtgatcaa gcttgccaaa
    26641 tcttcaatct tccaatgacc aaaacggtgg tcacggtctg caggctggcg cgccatccga
    26701 atcccgatta agagggccac atttccaatg atgtccgata cgttattaaa accatcggcc
    26761 accaaactgg atgaatgaag gagatgacca gctgctaatt tggctgcaga caagatcaaa
    26821 taggtcgaaa tactgataat ggctccacgc tcagctaact tgagatttga gatagattgc
    26881 ttcattcaac ttcctttcta gtcacatagc attctaccat tatactggtt ttcaaggaaa
    26941 aattcaaata aggcagtttt tactctttga aaactcccca aaatttggta taatggtact
    27001 actcaaggga gtagctggca gaaacctgtg atagtgtcgt cattccgaat tttatgctga
    27061 aaagtatgct ttccggccct atcttaaaca gcgagacttg ttatgattaa caggtctctt
    27121 tttgtttgtc gtgaaaagat atgacagaaa gagagtctgt tttgcacaca atgtcaagga
    27181 ggagacacat gtcaaaagaa caaaaacgcc aagcgtttta tactcagagc cctgaagagg
    27241 tcttgcaggc tgtggatgcg accgagcaag gtttgtcatc aagtgaggcg gaaaagcgcc
    27301 ttgctgaatt tgggcacaat gaactcgaag aaggcgagaa acgatcaatc ttggtcaaat
    27361 tcatcgagca atttaaggat ttgatgatta tcatcctagt tgcggcagca atcttgtcag
    27421 tcgtgacttc tggtggggaa gatatcgcag atgccattat catcctagct gtggttatca
    27481 tcaacgctgc ctttggtgtt taccaagaag gaaaagctga agaagctatt gaagccctca
    27541 aatccatgtc tagtccagtt gcccgcgttc ttcgtgatgg acacatggca gagattgact
    27601 ctaaagaatt ggtaccaggc gatatcgttg cccttgaagc aggtgacgtg gtaccagcgg
    27661 acctacgttt gatagaagcc aactctctta aaattgaaga agcagccttg acaggtgaat
    27721 ctgtaccagt cgaaaaagac ttgtcagtcg agcttgcgac agatgctggt attggtgacc
    27781 gtgtcaacat ggccttccaa aactcaaacg taacctatgg tcgtgggatg ggtgttgttg
    27841 tcaatacagg tatgtacact gaagttggtc atattgctgg tatgcttcaa gatgcggatg
    27901 agactgatac accactcaaa caaaatttga acaacctttc taaggtcttg acctatgcta
    27961 tcttggtcat tgcccttgtt acttttgtag tgggtgtctt cattcaaggg aaaaatccac
    28021 ttggtgagtt gttgacttct gttgcccttg ccgttgcagc cattccagaa ggacttcctg
    28081 ctatcgttac catcgttctt tctcttggta ctcaagtttt ggccaaacga cattccatcg
    28141 ttcgtaagtt gccagcagtt gaaacacttg gttcaactga aatcatcgct tctgataaga
    28201 ctggtacgct gactatgaac aagatgacag tcgaaaaagt cttctacgat gcggttctac
    28261 atgactcagc tgatgatatt gaactaggtc ttgaaatgcc actacttcgt tcagttgtct
    28321 tggccaatga tacgaaaatc gatgtggaag gtaacttgat tggtgaccca accgaaacag
    28381 cctttatcca atatgccttg gacaagggct atgatgtcaa aggtttctta gagaaatatc
    28441 ctcgtgtagc tgaattgcca tttgactctg accgtaagct catgtcaaca gttcacccat
    28501 tgccagatgg tcgtttcctt gtagcagtca agggtgcgcc agaccaactc ttaaaacgtt
    28561 gtcttcttcg tgataaggct ggggatattg ctccgattga tgagaaggtt acaaatctca
    28621 ttcatacaaa caattctgaa atggctcatc aagccttgcg tgtccttgca ggtgcttata
    28681 agattatcga tagtattcca gaaaatctca cttctgaaga gcttgaaaat gatttaattt
    28741 ttactggttt gattgggatg attgaccctg aacgtcctga agccgctgag gctgttcgtg
    28801 tggctaagga agcgggaatc cgtccaatta tgattacagg tgaccaccaa gacacagcgg
    28861 aagccattgc caaacgtttg ggaatcattg acgcaaacga tacagaaggt cacgttttaa
    28921 ctggtgctga actcaatgaa ctgtcagatg aagaatttga aaaagtcgtt ggtcaatact
    28981 ctgtttatgc ccgtgtgtct ccagaacaca aggttcgtat cgtcaaggct tggcaaaaac
    29041 aaggtaaagt cgttgccatg acaggtgacg gtgtcaatga cgcgccagct ctgaaaacag
    29101 ccgatatcgg tatcggtatg ggaatcactg gtacagaggt ttctaagggg gcttctgata
    29161 tgattcttgc agatgataac tttgcgacta ttatcgtcgc agtggaagaa ggacgtaagg
    29221 tcttctcaaa cattcaaaag actattcagt acctactttc tgctaatact gctgaagtat
    29281 taaccatctt cctatcaacc ttgtttggtt gggatgtctt acagccggtt catcttttgt
    29341 ggattaactt ggtaacggat accttcccag ctatcgctct tggtgttgaa cctgcggaac
    29401 ctggtgtcat gaatcataaa ccacgtggac gcaaggcaag cttcttctca ggtggtgttt
    29461 tgagttctat catttatcaa ggtgtactcc aagcagctct tgttatgagt gtttatggcc
    29521 ttgcgattgc ttacccagtt catgtgggtg acaatcatgc tattcatgca gatgccctaa
    29581 cgatggcctt tgcaaccctt ggtttgattc agctcttcca tgcctacaat gtcaagtctg
    29641 tctaccaatc catcttgaca gttggcccat tcaagtctaa gacctttaac tggtccatct
    29701 tggtatcctt tatccttctc atggcaacaa tcgttgtaga accgcttgaa ggaatcttcc
    29761 acgtaaccaa actagacttg tcacaatggg gaattgttat ggctggaagc ttctcaatga
    29821 ttatcatcgt cgaaatcgtt aagtttattc aacgcaaact cggttttgac aagaatgcga
    29881 tttaagggag gtttcggatg tcagcatatc aattaccgac cgtatggcag gatgaagcta
    29941 gtaatcaagg agcttttacg gggctaaaca gaccaacagc aggtgcccgt ttcgaacaaa
    30001 acttgccaaa aggagaacaa gcttttcagc tttattcact gggaacacca aatggtgtga
    30061 aggttactat cttattggaa gaattactag aagctggttt taaggaagcg gcttacgact
    30121 tgtataagat tgctatcatg gatggggatc aattcggctc agactttgtg aagctcaatc
    30181 caaattccaa gattccagcc ttattggacc agtcaggtac tgaaaacgta agagtctttg
    30241 agtctgctca tattcttctt taccttgctg agaaatttgg agccttttta ccaagtaatc
    30301 ctgtggaaaa ggtagaagtt ttgaattggc tattctggca agcaggtgca gcaccttttc
    30361 taggtggggg atttggacat ttcttcaatt atgctcctga aaaattggaa tatcctatta
    30421 accgttttac gatggaagtg aaacgccagt tggatttatt ggataaggaa ttggctcaga
    30481 aaccttatat tgcaggcaat gactatacga ttgcagatat tgctatctgg tcttggtatg
    30541 gacagttagt tcaaggaaat ctttaccaag gttctgcaaa attcttggat gcctcaagtt
    30601 atcaaaatct agtaaaatgg gcagaaaaaa ttgccaatcg tccagctgtt aagcgtggct
    30661 tggaagtaac ttatacagaa attaaatagc aaaaaagtca tcttcggatg gctttttgct
    30721 acagttgagg gaaagggttt gcagttatcg gcttttgtgc tataattgct ataatatagt
    30781 aaaaagcaag aggagtgtga ggaagtggaa aagaaaattg tgaaggatat cttattttta
    30841 tctcaagtgt ctcatccggc aagtcaggag gacctttatc ttgccagaga tttgcaggat
    30901 acactcttag caaatcgtga tacctgtgtt ggtctagctg ccaatatgat tgggatgcag
    30961 aagcgcgtga ttatctttaa tcttggcttg gttcccgtgg tcatgtttaa cccagtgctt
    31021 ctgtcctttg aaggatccta tgaggcagaa gaaggctgtt tgtccttggt aggtgtgaga
    31081 tcaactaagc gttatgaaac cataaggctt gcctatcgtg acagcaagtg gcaggaacag
    31141 accattacct tgacaggctt cccagctcag atttgccagc atgagctgga tcacttggaa
    31201 ggacgaatca tttaggagga aagcaaatga aacgaatagt ctttgaactt atttttatcg
    31261 caacgacctg gtatatcttt ttaccgcccc ttaacctgac cagctgggaa tttctcttct
    31321 tcctctgtgg gcatttgtta gttgtggcaa tcttatttgg ctttggcaag gggataaacc
    31381 ttgtcaaaac ggttcatgtg cgccacggta aggcggaagc tgccttaaat cttgagggtt
    31441 tcaaaatcaa tcggttaggg aaaattctgt tagcttcgat tggaggaatt cttctcttgg
    31501 cagctttggt ttccttggta acttctagca tgtttcaggc taaaaattat gccaatgtag
    31561 tcacggttac ggaaaaagac tttactgaat ttcctaagag tgacaccagt aaggttccta
    31621 tcctagatag aagtactgct gaaaaaattg gagaccgcta cttgggttcc ctaaccgata
    31681 aggtgtcgca atacgtagcg gcagatacct atacccaatt gacaattgat gggaaacctt
    31741 atcgggtcac accactagaa tatgcagacc ctatcaaatg gtttaacaat caagccaagg
    31801 gaatcggtga gtatatcaag gtggacatgg taactggaaa tgcggatttg gtggacttga
    31861 agacaccaat caagtattca gactcggagt attttaaccg tgatgtcaaa cgtcacctgc
    31921 gcttgaagta cccgaccaaa atctttaaaa ctccatcttt tgaggtggac gatgagggca
    31981 atcctttcta tgtagcaacg gtttaccaaa agcaatttgg acttgctgtt cctcgtcctg
    32041 cttcagtcat tatcttggat gctacaaatg gagaaaccaa ggaatacagc ttatcagatg
    32101 ttccagaatg ggtggacagg atctatccag cagaggaaac cattgagcaa atcaactaca
    32161 acggcaagta caaggacggt ttcttgaatg ccatgatttc caagaaaaac gtgacccaga
    32221 cgaccaatgg ctataattac ttgtctatcg gtaatgacat ctatctctac acaggtgtga
    32281 cgtcggctaa tgcggatgag agtaatcttg gtttcatcct tgaaaatatg cgaacaggag
    32341 aaattactaa gtatagcttg gcttctgcga cagaagaatc agcccgtgaa tcagcagaag
    32401 gtgctgttca ggagaaatcc tacaaagcaa ccttcccaat cctcatcaac ctcaatgaca
    32461 agcctctcta catcatgggc ttaaaggaca atgctggctt ggtcaaagag tacgccctgg
    32521 tagacgcagt cgagtaccaa aatgttatcg ttgctactac agtggaagag atgctcagca
    32581 agtatgccaa taaaaacgac cttgaaattg acaatgcaac gacagaaagc atcaagggag
    32641 tagtagcaga cctcaaatca gctgttatca agggagacac tgtctacttc tttaaagttg
    32701 atggcaagat atacaaggta aaggcttcag tatccgatga ccttccttac cttgaaaatg
    32761 gtaaaacctt cgaaggtcaa gtaggaaaag acaattatct caagaccttt aaagtacaat
    32821 aatactcttc gaaaatctct agctctatct gcaacctcaa aacagtgttt tgagcagcct
    32881 gcggctagtt tcctagtttg ctctttgatt ttcattgagt ataaaaaagg tttatttaat
    32941 aaatatatgt tataataagg taaattaagc ctaatggagg acaaagaaat gggtttttat
    33001 ttgatggttg cttcaatgct actcggactg ctcgctttga agataggttt ttctcagttc
    33061 aaggagaaaa aagataaatt cttatctatc ttaacaagtt tagctggcac agcccttgtt
    33121 ctcgtggctg tctggttagg atggcccaaa taaatagaaa aacctcggtc aaaccgaggt
    33181 ttttcaaatg aatttcttgg ttgtggctaa aaaatatgct acactatcaa tatgaaaatt
    33241 ttaatcccaa cagcaaaaga aatgaacaca gacttgccaa gtatcgaggc aattccttta
    33301 aaaccagaaa gtcagaccgt gcttgatgcc ttggctctct attctgccag tcaattggag
    33361 agtttctaca aggtatcagc tgagaaagcg gcggaagaat ttcaaaatat ccaagctttg
    33421 aaaaggcaaa ctgctcaaca ctatccagcc ttgaaacttt ttgatgggct tatgtaccgc
    33481 aacattaaga gagataagct gaccgaggcg gaacaagatt atcttgaaaa tcatgtcttc
    33541 attacctcgg ctttgtacgg tgttgttcca gccttgtcac ccatggctcc tcaccgtttg
    33601 gattttttga tgaaattaaa agtcgctggt aagactttga agagccattg gaaggcagtc
    33661 tatgatgaag ctctgaagaa ggaagaagtg attttctctc tcttgtcatc agagtttgag
    33721 actgtatttt ctaaggaaat cagagcaaag atggtgacct tcaaattcat ggaggataga
    33781 ggcggtcagc tgaagattca ctcaactatc tccaagaaag cgcgcggggc ctttctaaca
    33841 gctttaatag aaaatcaagt acaaactgtg ggggaagcac gtcgcttgaa ctttgctgga
    33901 tttgtttacc gagaagattt gtcacaacca cagggattgg tttttgtaaa ggaagtatag
    33961 aaatcagata agagaaaagt cagcacgatt atgctgactt ttttgataac ttataaagta
    34021 ttggacaaat gaatctcatt tgataaggtg ttaagatgat taaccaaatt catgattgac
    34081 agatgatttc ggcaaataaa cttcttggta atccctgaaa tatagaaatt agacacgatg
    34141 atatcgtaat ctgtctgatt taaaatctca ggacttgttt ttaattcatc ccaaatatcg
    34201 aaggtaaagc ggttattaca gtaataggaa agcatatcaa caaaggtaag ggatatagca
    34261 tggtcaaagt tactgataat caaaaccttg ataggtggtc gattttctaa caactgagta
    34321 gagaggtttt cagcatgggt taagatggtg tacatcaagt gttccaactg ttcaggatgg
    34381 tcatgctgtc caattgcctg acggtattga gccagttctt gacgcgcact tcccataaag
    34441 tctgggaagt aaccttcaaa ttttttaatc gtcaatgctt tttgttcaaa taagattggg
    34501 gtagaaaaga tttcctgtct ttcaaagaaa gcagtgttgt gtaaatgcca aatcagctca
    34561 tcgtggttag taaaagtaat cttatattgt tttgataggc gttctaatat ttgactaagt
    34621 aattggtaag agtatcttgc ttgactgtta tcttctaaag agttgaagaa ttcttgagga
    34681 tctaggaaaa tatcattctg aagaaaagaa ataaaaattt gagcgattgt atctggagtc
    34741 atttcaagcc caaaatgttt agagaaataa gcaagtgtct cctggaaatt tggaatgctc
    34801 tttaaaagag gataatattc tttgtaaaag tggttgggga ggtcaatgaa gtgaccattt
    34861 ttaaccctgt ggatactgat agctatcatc agtttgtaca ttctataaat tgaaaatcgc
    34921 attggataat tcgtgatttt atagaagaag tcagcaaact ctgttaaatc ttcctcagga
    34981 aggtcaggaa aaggccaatc taggaaataa tagcgctcag aaaagtattg ggcaaaaaag
    35041 taacgaatat cgatctcatt tccacgtatc tcggatggag taaaagataa ttcaatttga
    35101 aactgagatg aaagaacctt tgtaatattt ctgcccagtc gatagaggtt ggcactacta
    35161 aagtaaagat tttctattgt tcgataaata ggtagcccct cattgaagaa catatactct
    35221 agtaattgaa aagattgaga attagcaaga aaatattgat aaatatcttc tacactagtg
    35281 ttaacttcta aatctatcat gattccgtta acagaagatt ggatgttaat agatggaaag
    35341 gcaatacgta attcatttaa atcacttttt aagattcgtt ctgtacatcc taatttttcg
    35401 gctagttcat gtaatttcat ccaattacga ttctgaatta gggtttccat taagcgtaat
    35461 tgtctttgaa cttttgtgga taaaaggttt ctcatacggg tatttcctcc ataattcatt
    35521 atatcttttt ttgtctaagt agtataaaaa aattagagca agaaaattgt cgttagtaat
    35581 cggaaaaatt agaataatat aagtggcttt taagtgcaaa aacagaatat attaattgtt
    35641 cagaaaatat tgattaatgc ctcaaatagt tgtacattgt aaactataaa atactatcta
    35701 ggagtttaaa atgaaacatt cacataaaaa atcatttgac tggtatagca tgcaacaacg
    35761 ttattctatt cgtaagtatt actttggtgc agttagtgtc ttgctcggta ccgctttggt
    35821 attaggtgca gcagctagtg tccaaacagt acaagcggaa gaaaacaaac aagaaactac
    35881 caatagtatt tctgttggta ggggagaagc agctactaaa ccagcagagg tttctgcgtc
    35941 taataaagag aaaacctatg cagctccaac tgtagctaat ccagtagaaa cgactccagt
    36001 taaaactgga gaagttacta aaccagcaga aaaagttgaa gaagcaaaag acaaaaaaga
    36061 ggaagtaacg catcaagatg ctattgacaa gtcaaaattg ttaacggctc tttcgcgtgc
    36121 taaaaagtta gaaagcaagt tatatacaga agcaagtgct gctaacttgc aaacaagtat
    36181 ccaagctggt caaagcttgc ttgggaaagc agatgcatct gaagctgaat tatcagcagc
    36241 agagtcatct attcaatcat ctattattgg tctagaactt cgttctaact ctaacaaagg
    36301 aactgtatca gaaactcctg tagcgaagaa agctaatata gttgaagcga aggaagagac
    36361 taatccagct gcaacaactg atcgttcagc tgttgatagt gctgttttgc caactagcac
    36421 agctgccaaa gtagaaacaa cttcagctcc agcatctatt aatgaaatct tgaaaccagg
    36481 tttgagcctt tctgatgctc gccaaaatcc agctatccgt aaggaagatg ttgatagagg
    36541 gtatagtggt tttagagcgg cgcctgctcc aacgaaccaa ccagtaacta atctaggaaa
    36601 taatactgtt ttcactgata ttagcagagg ttctcatact ttccgtggta atggaaattc
    36661 acgtcgtgga aacccgattc attttgatgt aacaacaact agagttagaa atagagtaga
    36721 tttctcaatc agatattcag gaccaggtga gtttgttaat aacaacttta tcttagacaa
    36781 aggaaatgga ttcggagaac cttctcgtgc aactgtttca acaccaaaat taagagacca
    36841 agttaaaaac attagtaagg gtggaaactt tgtttctcat tcaggctaca gtttagaagc
    36901 tgcagtagaa acaagcacca atcaaacaat taagtttagt ttacctatag ccaaccctaa
    36961 tggtgatctg tctgttcgat taagacctgt tacttttaat gtaggtcaag gggaatcagg
    37021 atctgcaact agtaatgatc catatagtaa ctctaactat tacttcagag aaaaaccact
    37081 cctcttggat gcaaatccaa atggtggtac taataataag actgtttcag aagacattga
    37141 cttccaaact gtctatcttc caactagtaa gttaccagaa ggtcaaacta gattagttcg
    37201 agaaggtgaa aaaggacaac gtcaaatcat ctataaagtt catcaattgg gtaacgaaac
    37261 aatcctagga ttgccaatta gtaattcagt tattaaagaa gttaagccac gtattatgca
    37321 aattggtgtg gctaaagatc taatcgatac agtaaaacca cgtgttgatc aaaataaagt
    37381 cggtgataca aataacctca ctttctatct tgataacgat ggaaacggtg tttatactga
    37441 gggcgtagac gaacttgttc aaaaaattgc tattaaagat ggtgctaaag gtgaaaaagg
    37501 agaccgaggc gaacgcggtc taactggagc tcaaggagct aaaggtgaaa aaggagaccg
    37561 aggtgaacgc ggtctaactg gagctcaagg agctaaaggt gaaaaaggag accgaggtga
    37621 acgcggtcta accggagctc aaggagctaa aggtgaaaaa ggagaccga
//
LOCUS       DAVBBS010000014        37450 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVBBS010000014 DAVBBS010000000
VERSION     DAVBBS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37450
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.014"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..775)
                     /locus_tag="VQU32_000701"
     CDS             complement(<1..775)
                     /locus_tag="VQU32_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2472188.1"
                     /translation="MSKETVVYTKRREKIKDNILGYSFLAPALILLGIFLVIPVGMVI
                     YYAFTDYYLLTPDERKFVGLENFIRLTKDPIFLKSFLNTLKFVVWIIPVQLGAALGMA
                     LIVNKKRKGNMFFKVAFFAPVVMSLVVISILWLYLLNPNSGLLNAILNKVGIASQPFL
                     TSPKQAMYAIVFVSAWQGAGYQMLLFLGGMQNIPQDVYEAAELDGFSKWAQFRYITMP
                     LLKPTALFVLLTTLISAFKLIVQPMVMTQGGPLNSTITMV"
     gene            complement(871..2181)
                     /locus_tag="VQU32_000702"
     CDS             complement(871..2181)
                     /locus_tag="VQU32_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET2472189.1"
                     /translation="MRKGTMLSVVAGLSLAILAGCSGGTNSKQASSSNDIKVWVQFSD
                     ETNEGKAWQKVVDNFNQSGKSKYKVVTEYIPRSGSGGGYEDKVNAAITTNSLPDVITL
                     DGPNTAAYAKSKVITPLDDYLKDNNMDDVLDSIKQQGTYNGKFYAFGFSESNVGVYYN
                     KKMFKEAGIAESELPTLEKPWTWDEFNTIAKKLKDHYNKPAIDFRINSNDEMLPYAYM
                     PLIWSNNGSVVNEDGTKAEGYFNSKESVEAVQFIQNLVKEGYTTVSPVEKGFETGEYP
                     MLLSGSWTIADMNTNYKDIDFGILPYPVSSKTKKLVSPSGSWQLAVTTKSDKKEAASE
                     FVKFATNTESSEIISLGNSVLPIRKSTIENIKDKVSEPMRFLMEQNSKTAHARPVVVA
                     YPQVSRAFQQAMQDISYYEENPNVQKVLDTRTKEMQTAIDQSLK"
     gene            complement(2308..3303)
                     /locus_tag="VQU32_000703"
     CDS             complement(2308..3303)
                     /locus_tag="VQU32_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2472190.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLRKVQR
                     AIKTLDYIPDNYARGMKKNRTETIALIIPSIWHPFFSEFAYHVEKELLKYNYKLFICN
                     ADSDSQNEIEYIEMLKQNKVDGIIGITYSDIDKYILSDLPFVSIDRHFSENVCYVTSA
                     NYKGGQTAAEELVRRDNKHLAFIGSINMYENETILRSKGFREKAKELGVDVYNFEMKE
                     PLINPEDQIRIFLQSHKEIDGIFTINDMMALRVISVMKELGKIAPDDYQIIGFDGLKT
                     SVDQSYLVSTIVQDIGMMAKESVAMILKMIKREEILEKRIVIPTYFGGDKTTKKI"
     gene            complement(3665..5146)
                     /locus_tag="VQU32_000704"
     CDS             complement(3665..5146)
                     /locus_tag="VQU32_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2472191.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSS
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            5589..5753
                     /locus_tag="VQU32_000705"
     CDS             5589..5753
                     /locus_tag="VQU32_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET2472192.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            5837..6403
                     /gene="amaP"
                     /locus_tag="VQU32_000706"
     CDS             5837..6403
                     /gene="amaP"
                     /locus_tag="VQU32_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HET2472193.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            6415..6585
                     /locus_tag="VQU32_000707"
     CDS             6415..6585
                     /locus_tag="VQU32_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HET2472194.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            6624..7232
                     /locus_tag="VQU32_000708"
     CDS             6624..7232
                     /locus_tag="VQU32_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2472195.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            7263..7466
                     /locus_tag="VQU32_000709"
     CDS             7263..7466
                     /locus_tag="VQU32_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET2472196.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(7576..7854)
                     /locus_tag="VQU32_000710"
                     /pseudo
     CDS             complement(7576..7854)
                     /locus_tag="VQU32_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8052..8339
                     /locus_tag="VQU32_000711"
     CDS             8052..8339
                     /locus_tag="VQU32_000711"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2472197.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCSRQRRLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(8617..9387)
                     /locus_tag="VQU32_000712"
     CDS             complement(8617..9387)
                     /locus_tag="VQU32_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET2472198.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(9402..10250)
                     /locus_tag="VQU32_000713"
     CDS             complement(9402..10250)
                     /locus_tag="VQU32_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HET2472199.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDRFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(10247..11722)
                     /locus_tag="VQU32_000714"
     CDS             complement(10247..11722)
                     /locus_tag="VQU32_000714"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HET2472200.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYVSDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTSLKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(12045..13400)
                     /locus_tag="VQU32_000715"
     CDS             complement(12045..13400)
                     /locus_tag="VQU32_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2472201.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(13410..13727)
                     /locus_tag="VQU32_000716"
     CDS             complement(13410..13727)
                     /locus_tag="VQU32_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2472202.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(13744..14061)
                     /locus_tag="VQU32_000717"
     CDS             complement(13744..14061)
                     /locus_tag="VQU32_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2472203.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(14074..15759)
                     /locus_tag="VQU32_000718"
     CDS             complement(14074..15759)
                     /locus_tag="VQU32_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HET2472204.1"
                     /translation="MKNKKLISSLFILFLLIFIHVKVFANENLIKNSNFEQGELFWNS
                     RNRGIINSNESYSNGHYSGMIPSTAINDNRADGYIGQVIDIEPNEDYLVSAFAKVDIS
                     GAEGYFTARWFDNNKQGEIVQNNIGEAVDQTVNTTEWKKYMFSFNSGKHDKVLIQLVK
                     WSEDDTTKKSNIFIDNVEMYQLSKGNSYKKIWRDDFDGEQLNKKYWGYELGSIRGWEQ
                     QHYVRSDENVFLRSGNLVLRATNRSKEDQYFNPRNNHRKVVYNSGSVRTHGKVEFLYG
                     KLEMRAKLPKGQGVFPAFWTLGSDFTLDGKINPVQGRGWPSTGEIDIMELVGERNSNG
                     SGNKTVYQTLHYGQSDNDDGKFAGRGTAFKLSSGNFNDSYHTFSIDWYKDYIVWMVDN
                     HIVRKVYYGSDNIAKKIFNRPQYVQLNLAMGGSWPGVVDNNLSGTEFVVDYVSYSRND
                     LQQHQAEEYYSQSPKINGAKDIVINRGDVPDLLKGITTNSGYELDYSIENEPMFNNFG
                     GNTSVDLLIRNKTEKEKISQMPPGVYNLHYTSRPKHLDYESKVDRKTVTLTIK"
     gene            complement(15764..16660)
                     /locus_tag="VQU32_000719"
     CDS             complement(15764..16660)
                     /locus_tag="VQU32_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HET2472205.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(16663..17925)
                     /locus_tag="VQU32_000720"
     CDS             complement(16663..17925)
                     /locus_tag="VQU32_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2472206.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFITGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQGALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPSWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            18205..19383
                     /locus_tag="VQU32_000721"
     CDS             18205..19383
                     /locus_tag="VQU32_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET2472207.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
     gene            complement(19611..20057)
                     /locus_tag="VQU32_000722"
     CDS             complement(19611..20057)
                     /locus_tag="VQU32_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2472208.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLPLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSGLKLE"
     gene            20127..20786
                     /locus_tag="VQU32_000723"
     CDS             20127..20786
                     /locus_tag="VQU32_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET2472209.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTPLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCVIIFGKLLL
                     V"
     gene            complement(20838..21989)
                     /locus_tag="VQU32_000724"
     CDS             complement(20838..21989)
                     /locus_tag="VQU32_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET2472210.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNITIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(22004..22735)
                     /locus_tag="VQU32_000725"
     CDS             complement(22004..22735)
                     /locus_tag="VQU32_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472211.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            22964..23065
                     /locus_tag="VQU32_000726"
                     /pseudo
     CDS             22964..23065
                     /locus_tag="VQU32_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            complement(23037..23813)
                     /locus_tag="VQU32_000727"
     CDS             complement(23037..23813)
                     /locus_tag="VQU32_000727"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET2472212.1"
                     /translation="MPKTLTKKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(23806..25029)
                     /gene="trpB"
                     /locus_tag="VQU32_000728"
     CDS             complement(23806..25029)
                     /gene="trpB"
                     /locus_tag="VQU32_000728"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET2472213.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGFGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            complement(25007..25606)
                     /locus_tag="VQU32_000729"
     CDS             complement(25007..25606)
                     /locus_tag="VQU32_000729"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET2472214.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVAADLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            complement(25593..26360)
                     /gene="trpC"
                     /locus_tag="VQU32_000730"
     CDS             complement(25593..26360)
                     /gene="trpC"
                     /locus_tag="VQU32_000730"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET2472215.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDC
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNTGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            complement(26357..27361)
                     /gene="trpD"
                     /locus_tag="VQU32_000731"
     CDS             complement(26357..27361)
                     /gene="trpD"
                     /locus_tag="VQU32_000731"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET2472216.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEDYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            complement(27372..27938)
                     /locus_tag="VQU32_000732"
     CDS             complement(27372..27938)
                     /locus_tag="VQU32_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET2472217.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMICDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            complement(27935..29296)
                     /gene="trpE"
                     /locus_tag="VQU32_000733"
     CDS             complement(27935..29296)
                     /gene="trpE"
                     /locus_tag="VQU32_000733"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET2472218.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQAIDRDPLDFLYEVTHKSQHHSDLPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRSVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            complement(29797..29874)
                     /locus_tag="VQU32_000734"
                     /pseudo
     CDS             complement(29797..29874)
                     /locus_tag="VQU32_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(30479..31480)
                     /locus_tag="VQU32_000735"
     CDS             complement(30479..31480)
                     /locus_tag="VQU32_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2472219.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            complement(31588..31956)
                     /locus_tag="VQU32_000736"
     CDS             complement(31588..31956)
                     /locus_tag="VQU32_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472220.1"
                     /translation="MKDYKINFDLGKIEYFDNNCLIQVYKFISFYDICEMVFAFHLPP
                     DELITNVIFKEKINSMLKCYIDRLLYVFINPTHFTEKVNLQFYGSFFSYEFICREVGN
                     ILKNKGVKCNLNFFEGEEYL"
     gene            complement(32052..32762)
                     /locus_tag="VQU32_000737"
     CDS             complement(32052..32762)
                     /locus_tag="VQU32_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET2472221.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
     gene            complement(32765..34456)
                     /locus_tag="VQU32_000738"
     CDS             complement(32765..34456)
                     /locus_tag="VQU32_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2472222.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDPNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            complement(34468..35478)
                     /locus_tag="VQU32_000739"
     CDS             complement(34468..35478)
                     /locus_tag="VQU32_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2472223.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            complement(35581..36648)
                     /locus_tag="VQU32_000740"
     CDS             complement(35581..36648)
                     /locus_tag="VQU32_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2472224.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            complement(36673..37332)
                     /locus_tag="VQU32_000741"
     CDS             complement(36673..37332)
                     /locus_tag="VQU32_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HET2472225.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
BASE COUNT        12046 a         7633 c         5729 g        12042 t
ORIGIN      
        1 acaccatagt aattgttgag tttaatgggc caccttgcgt cataaccatt ggttgtacaa
       61 tcagcttaaa agcagaaatt aaagttgtta acaatacaaa cagggctgtt ggttttagta
      121 ggggcatcgt gatataacga aactgagccc attttgagaa tccatctaat tcagcagctt
      181 catagacatc ttgtggaata ttctgcattc ctcctaaaaa taaaagcatc tgataacctg
      241 caccctgcca agcagaaaca aaaacaattg catacatagc ttgtttagga ctagtcaaga
      301 aaggttgaga agcaatacct accttattta aaatagcatt taataagcca ctatttggat
      361 ttaaaagata aagccaaaga atagaaataa ctaccaagga catgacaact ggagcaaaaa
      421 aagccacttt aaaaaacata tttccttttc gctttttatt gacaatcaaa gccattccta
      481 aggccgctcc caattgaact ggaataatcc aaacaacaaa tttcaaagta ttcaagaagc
      541 tctttaagaa aatcggatct tttgttaatc gaataaaatt ttcaagtcca acaaattttc
      601 tttcatctgg tgtcaacaaa taataatctg taaaagcgta ataaatgacc attccaactg
      661 gaattactaa aaagatacct aataaaatta aagctggagc taaaaaacta taacctaata
      721 tgttatcctt tattttttca cgtctttttg tatacacaac agtttcttta ctcatatcca
      781 tctcctttaa tcatatatat caaactaggc tattaacaag aaaggggaag gtagttccaa
      841 acattttcct tccaccttct tttttacaga ttatttgagt gactgatcaa tagcagtttg
      901 catttctttt gtacgagtat ccaagacttt ttgcacattt ggattttctt cataatagct
      961 aatatcttgc attgcttgtt gaaaagctcg agaaacctga ggatatgcta cgacaactgg
     1021 gcgagcatga gctgtttttg aattttgttc catcaagaaa cgcattggtt cggatacttt
     1081 atctttgata ttttcaattg tagatttacg aattggaagg accgaattcc ccaagctaat
     1141 aatttcactt gattctgtat ttgtagcaaa tttaacaaat tcagatgcgg cctctttttt
     1201 atcagatttt gtcgtcacag ctaattgcca actacctgaa ggggatacta attttttcgt
     1261 cttactagat actgggtaag gaaggatacc aaaatcaata tctttgtagt ttgtattcat
     1321 gtcagcaatc gtccaagaac cactcaaaag cattggatac tctcctgttt caaaaccttt
     1381 ttcgactgga ctaacagttg tatagccttc ttttacaaga ttttgaatga attgaactgc
     1441 ttctacactc tctttggagt tgaagtatcc ttctgccttt gttccatctt cgtttacgac
     1501 agaaccatta tttgaccaaa tcaacggcat ataagcatat ggcaacattt catcgtttga
     1561 attaatacga aaatcaattg ctggtttgtt ataatgatct ttcaattttt tagcaatcgt
     1621 attaaattcg tcccatgtcc atggcttttc tagcgttgga agctctgatt ccgcaattcc
     1681 agcctctttg aacatttttt tattgtaata aacaccaaca ttcgattcag aaaatccaaa
     1741 tgcatagaat ttcccattat aagttccctg ttgtttaata ctgtccaaaa catcatccat
     1801 attattatct tttagataat catctagcgg agtaattact ttagattttg cataagctgc
     1861 cgtatttggt ccatcaaggg taataacatc tggaagacta ttagtcgtaa tagcagcatt
     1921 aaccttgtct tcatatcctc caccactacc actacgagga atatactcag ttacaacctt
     1981 gtacttactt tttcctgatt gattaaagtt atcaacaact ttttgccaag cttttccttc
     2041 atttgtttcg tcagagaatt gtacccagac tttaatatca tttgaagaac tcgcttgttt
     2101 tgagttagtt cctccactac aacctgcaag tatagctaac gatagccccg caacaacaga
     2161 taacattgta cctttacgca tcatttttct cctttttgaa acgtttcatt ttttatttat
     2221 tttttaaaca agcttctagc ctatttttga aacgtttcat ttgttgatta tattataaca
     2281 gcgctttcat taattgtcaa gcgcttttta aatttttttt gttgttttat ctcccccaaa
     2341 atatgtggga attacgattc gtttttctag aatctcttct cgcttaatca ttttcagtat
     2401 cattgcaaca gattcttttg ccatcatacc aatatcttga actattgtag acactagata
     2461 agattgatcg acagaagttt ttaaaccatc aaacccaatt atctggtaat catcaggtgc
     2521 tattttcccc agttctttca taacactaat aactctcaga gccatcatat catttattgt
     2581 aaatatacca tcaatttctt tatgagattg caaaaaaatt cttatctgat cttcaggatt
     2641 tattaaaggt tctttcattt caaaattata gacatctact cctaattcct tagctttttc
     2701 tctgaatcct ttacttctga gtatagtctc attctcatac atattaattg aaccaataaa
     2761 agcaaggtgc ttattatccc ttctaaccag ttcctctgct gcagtttgtc ctcctttgta
     2821 atttgctgaa gtaacatagc atacattttc cgaaaaatgt ctatctatac ttacaaacgg
     2881 taaatcagat aaaatatatt tgtcaatatc agaatatgta ataccaataa ttccatccac
     2941 tttattttgt ttcaacattt cgatatattc gatttcattt tgtgaatcgc tatcagcgtt
     3001 acaaataaat aatttataat tatattttaa taactctttc tcaacatgat aggcaaactc
     3061 cgaaaaaaat ggatgccata tgctcggaat aatcaaagcg atagtttctg ttctattctt
     3121 tttcatacct cgagcataat tatcagggat atagtccaat gtttttatag ccctttgaac
     3181 tttccttaaa gttacttctt taatgccttt ttctttatta attacacgtg aaacagttcc
     3241 aacactaact cctgcttcta aagcaacatc tttcatggta attgattttc tttgttctac
     3301 catattatca cctcctttca atatatagta tcatgcaaat gctttttaag caactatttc
     3361 tcaatcattt ttggccagat catttatccc atcatgaata aaatcactcc aattagcttt
     3421 tgaaaatact tcaattttca tgtgtaaaca tctacataaa acaggaaaag ccttggtttt
     3481 atggcttttt tcgtatcttc tacaaaaaaa gcaagagttt tagagggcta taaatctaga
     3541 tgtacatttt gcttaaatga ttgaaggtct tttcttaaca aaaacacccc aaaaattaga
     3601 ttttttctgt ctaacttttg aggtacagtt caaacgcgaa atagcgtttt tttgttattt
     3661 ttggttactc atctaatcga ataaacatca tggcatttaa caaatatatg agtgagaccg
     3721 tgtttatatt atttgaatag atgagtctct tattttcaat aggaggaata ataaaattag
     3781 aaataatgat atcataagat gaatcttcta aagattcctt tgataattct aattcagtcc
     3841 aaacttccag ttcaaaatta ttgctacagt aataagaaag tgtctctgca acgaattttg
     3901 catgatactg atcaaaatta ctcataacca aaacctttag tttaggctga ttttgtagca
     3961 aattaatcac caaatgtttg gtatgagtga tgaaggtata agatagatga tttaccatca
     4021 ttgaactaga acaaacctca agagtctcta aatagtgaga aagctctttt tttatatctg
     4081 aaacaaattt tggaaaaata ttttgaaagt tcctgattgt attccctttt tgatcaaata
     4141 aaataaactc agtaaacaac tcttgacgat acagatgtgc ggtattatgc agatgccaaa
     4201 tcagattatc cttattctcc atttcaatct gatacttgac tgaaatctga tcaataaaat
     4261 cactcaatag atggtaagat ttttcaacat aactatcctt ttttacgcat ttcataaaga
     4321 gactttcatc tatgaaaaac attttttgaa agtaagacac aaataattgg caaacaactt
     4381 cttcatctaa agagatattg tattctgatt caaaactctg agcaacacct tctattcctt
     4441 ctgcctgcat taaaaaatcc aaactttggt cgttaaaaga atctttatct acttccataa
     4501 aatgaccaaa ctttattcta tataggttcg taactaggag caactttagc attctatgcg
     4561 ttgacaaatt cattggaaag cttgtttcct tataaaccaa ttctaacaat tgagatagtg
     4621 gctctgatga aaaattttca aatggccatt ctaggaaata atatttttct gaaaaatatt
     4681 gtgcaaaaaa gtaacgaatg tctctctcat ttccaatgat ttgaacaggg gtcagactga
     4741 cttcaaattg aaattgcctt ttaatcactt tattgatttg gctaataata cgatagagcg
     4801 aagatgaact gatataaaat tctttacaaa tactctcagc ttgacaacct tcattaaaga
     4861 agatgaattc taaaatcgaa aaatgagttg aatgtttaaa gaaatgatgg taaaccattt
     4921 caatatcact atcatcggta ttaataatgc gtataccatt agtagaagaa tgaaaaatca
     4981 agtcaggaaa agcagattta acatgggata gatcatcttt gactgcacgt tctgtacaat
     5041 ttaataactc tgctagttca gaacgatgaa accaacgttt atgttcaaat aataattcta
     5101 ataattctaa ttgcctatga ctttttttag ataataaatc tctcatgaat atctttctct
     5161 ctttataaat tatcggatta aacctcttgc aattatacca caaagaatag gtatagcatg
     5221 atataacgac ttttcctaaa atcttttatt tcgtataata acactacgga gacaatatat
     5281 aaacaatttt cttattttac cgtctattga gggcgtgaat acagaatcaa attcaagtct
     5341 aaagattata tttttaattt taaaaattat ataatagcaa caattaaaga atttgatttt
     5401 ttaaaattat ataataataa caatcgaaat aattgacttt tctatattaa agttatataa
     5461 tagtattaat caaagaaatt gattttttga tattaaaata aaaaaggagg gtaggcagtg
     5521 ttgtgatcaa ttattgctgg aggtcttatt ggtctcttgg caggtaaaat cactaaaaaa
     5581 gtagttctat gggaatcatc gcaaatgtat tcgctggttt agtcggggca tatgcaggac
     5641 aatctctttt aggtagttgg ggtccatcaa tcgctggaat ggctttgctc ccatctattg
     5701 taggtgcagc gattgtgatt actgtagtgt cattctttac aggtagaaag taaacttttc
     5761 gccagtaaag ttagcaaact atttttaaat caatgacggg aaaaatagtt taaatgttaa
     5821 atcgaaagga ttgtatatgt caaaagcaaa gaaaatatgt ttcattattt tctgtatttt
     5881 aatcttgaca attttccttc ctgttttgat agattatcat caagttagtg atctaggtat
     5941 tcatctactt agctggagac agaactccgt agttgaattc tatcttgcta gatatgtctt
     6001 ttgggggaca gtggttctat caactttagt tttattatcc attttagttg tgatgtttta
     6061 tcctaaacgt tacttggaaa tccaacttga aactaaaaac gatacattaa aattaaagaa
     6121 ttcggcaatc gaaggttttg ttagaagttt ggtgagtgat catagattga tcaagaaccc
     6181 aactgttcat gtaaatttac gaaaaaataa atgtttcgtt catgtagaag gtaaaattct
     6241 tccttcagac aacatcgctg acagatgcca aataattcaa aatgaaataa ctaatggatt
     6301 gaagcagttt tttggtattg agcgtcaagt aaaacttgaa gttgcagtaa aaaattacca
     6361 accaaaacct caaaacaaaa agactgttag tcgtgtgaag taaggaagta aaaaatggaa
     6421 tggcttaaac aatatcgata tccaattatc gctggtctca taggcgtatt tctggcttgt
     6481 ttgattgtct cctttggctt cttcaaaaca atatttgtat tgattttagg agcactggga
     6541 gttgcagctg gattatatat cgaaaaaaac tatatagata aataaaaaaa taaaaattac
     6601 taatttaatt aaaggagttt catatgtcaa acgaaaaaaa cacaaatact aacgtagaaa
     6661 agaaagatgc tactgttgta gctcacgaaa tcaaagggga acttacttac gaagataaag
     6721 ttatccaaaa aatcattggt ctttcactag aaaacgtttc aggtcttttg ggaatcgatg
     6781 gtggtttctt ctcaaatctt aaagaaaaaa tcgttaacag cgatgacgta acaagtggtg
     6841 ttaacgtaga agttggtaaa acacaagttg cagttgactt aaacgttatt gttgagtacc
     6901 aaaaaaatgt tccagcttta tattcagaaa tcagagaaat cgtatcttca gaagttgcta
     6961 aaatgactga cttggaaatt gttgaaatca acgtaaacgt tgtcgacatc aaaactaaag
     7021 aacagcatga agcagactca gtaagccttc aagatcgcgt atctgacgtt gctgaatcaa
     7081 caggagaatt cacttcagaa caattcgaaa aagctaaatc tggtcttgga tctggtttct
     7141 caactgttca agaaaaagtt agcgaaggtg tagaagctgt taaaggtgca gcaaatggtg
     7201 tagtatctca cgaaaacact cgtgtaaact aagataaaat aaatataaca ggagaaatta
     7261 tcatgtcagt agaagaaaaa ttaaatcaag ctaaaggttc tattaaagaa ggtgttggga
     7321 aagccatcgg tgatgaaaaa atggaaaaag aaggagcagc tgaaaaagtt gtttctaaag
     7381 taaaagaagt tgccgaagac gctaaagacg ctgtagaagg tgctgtagaa ggtgttaaaa
     7441 acatgttgag tggcgatgat aaataaggtt aaaagttact ttatcttttt ggtaacatta
     7501 gtcaaaagag tctgagtcaa gatgattctc agaaaacaaa aagctagaga ttcccaattg
     7561 cggaactcta gctttttaat tttgcctctt tctcttatta tatttcagca ggttgttggc
     7621 catgagtatg aatcccatgt caattctcac ttgacgctta cctctcagat gacatctctt
     7681 ataacccaaa caaaccttta tctgcccaaa gacagatttc atatcaatct tacgtttagc
     7741 gaaaatttgt ctacccttgg aagataaaag tgcctgatat tctttagttt ttaaacactg
     7801 gtaacgttca ttcatataca gtcccttttg aggggctgat tcaggttcat aatcgcagtc
     7861 aacattgatt tcaaggctgt ttgctttcta tctccccttt ttcatgatat attttcgtac
     7921 aggttcttca atagtttcta tagtaaaatg aaccaaaaat agtacacgtt gcggtataat
     7981 gttcttgtgg catattcatt agattttcgt aaacagttct ctcctatttg tgagcgaaca
     8041 agtatagtca attgattgtc agtttgaaga ccaagagtcg taaggcaaag gatatggcag
     8101 aatccatcca agggtggctg gcacaatttc tagtgaacct ctttaaatct attacgtttg
     8161 actgtggaaa agagttttca aaatggaaag atatttccaa tcatcacgat agtgagagct
     8221 tttttgcgaa tctaggatgt tcaagacagc ggcgtctcaa cgaacattca aatagacttt
     8281 taaggtgtca tgacttgcca aagcagacag atttcaacga agtcagtcaa gaattttagg
     8341 ctggacaaaa actcaatttc aggagaaaat gaaataaata ttcccacaat aaaacgcata
     8401 gtatcaaagt ttttccaaca cctgatatta tgcatttttt aattttaaag actttttccc
     8461 agcctcaaga aatcacatcc cacctaaatc ctttggatac caaatacctc atcaggtatt
     8521 ttctgagttg gtaagaatgt ctagcttaat ttgacaaatg gagggcgtta aatccagttt
     8581 ttacaggaag tctatttaaa tattttcaca ctgaccttac aaaatatgaa ataaattata
     8641 taaagtcatt cccatgacaa agaataacat ccctttaaaa cagtatctta cccaattttc
     8701 aggtaagact tttgatacaa ccgtccccaa tacccctccg catatagaag caagaaaaat
     8761 aactggaaca ggtgctaaat gatactgatt taaacctgta accacaacaa ttgtagccaa
     8821 atgagacagc tgggaaaaga aaataattgc aagagaatac atcgttgctt cttttatcga
     8881 aatagaaaaa aacaccatca ataaagatac attgagcgga ccaccaccaa tccctaaaaa
     8941 actagaaatt aagcccaata gaagaccaca aatcatatag aatcctatct tatcaaattg
     9001 ataagttttt ttaaaatctg ttaaaacaaa tgatacaagt agcatcacaa atagtaaaat
     9061 catttgaaca atggatacta gccgtactga taactgagat aacaaaactt gaaaaataag
     9121 atgacctaga aatcctccta aaacagaaga aaaggatacc gtaaaaatca atctccattt
     9181 tatctcttta ctttgtgaaa atcttttgat tgttgaaata atcgccatta taaaaacaga
     9241 gaaactagaa taaaaagcaa tatcactgac cgaatggtat ccaaaactat ccataagtgg
     9301 cttaattata attccaccac ccatgcctga tatagaaccc aatccactag ctattataat
     9361 gattagaaaa taaataatct caaccacttt ttaagtccat cctattcttt caaacatcta
     9421 aaacctagat ggctactagt ggaagtagag atacatccat ttctagcagc tactcggtat
     9481 cgattacaat aagaacaatg acataaaaag gaacctcctc tgatagcata ctcctcatct
     9541 tgtatcccat attttggcaa ctcatagtca tcttcattaa aactagctaa taaggtatat
     9601 ctcggattgc gacaccattc ccaaacattc ccaatcattt gatatagacc aaaatcatta
     9661 ggtaaaaatt catagacagg agccgttcct ataaatctat ctaaggcagt attttcataa
     9721 ggaaatcttc cttgccaagt attggcatga taatatccac cttctaaaag agtatctccc
     9781 caaggatatt cagattgtct tcctcctcta gcagcatatt cccactgtgc ttcagtaggt
     9841 aaagacattc cgctccaatt acagaaagcc aatgcatctt ctaaagcaac atgaactact
     9901 ggatggtctt ctaaacccac tagattactg ttctcaccgt agggatgttt ccagcaagcg
     9961 ttcggaactt gtaaccacca cggagcccca gcaggatgag gatatccctc cctttctgcc
    10021 tctggaacga acaatataaa aacaaaagac cactcttgtc gctcagctag tgttttatag
    10081 cctgtttctt tcacaaattg agcaaattct tgattcgtaa ctggagtatc cgcaatggaa
    10141 aaatccttta cactaacagc gacttgagga ccttcaaaat catctaaaaa gccctcttca
    10201 gaatttgttc ccatcataaa tgtcccaccc ttgatttttt tcattttcat gattgactat
    10261 cccccgtttt cgttagagtt ggcgttattt tagaaagttc aacaggaact aactcttcat
    10321 ccacaacaaa accttcttct ctatatctaa gaaagtctac taattttttc ttcatctgac
    10381 gaacaatagg ttggtatttt tcgcttggat acaaatcatt catttcatgc ggatcttttt
    10441 tcatatcaaa taactgataa tgatttaaaa caggaaacca gataaatttc cactggtctg
    10501 tcaagatata ttgactagaa tcttttccta aagcatgttc accatgaaat tctgttcgcc
    10561 acccctcgta ctgtccaaat aataaattct taacactccg tccatctagt tcatctgtag
    10621 tagttcctcc tgccaaatcc acgagtgaag gaaaaatatc ttgaattttc actaattgct
    10681 tgattgttcc cctattacca gctatcaatc cagccggatc gtaaataaac gaaggaatgt
    10741 ggatacttcc ctgataagga tatccctttc taaacagata atgttcacca agctgatctc
    10801 catgatccga aacaaaccag ataatcgtat ctttatcatg acgaaattct tttagggaag
    10861 tcaaaaagcg actaatttga tggtctatat gagtaatcaa tccaaaataa gcagcaacca
    10921 ttcttctttg atcatcttcc tttaatttcc ctcttaaagc ataaatagac ggaatttgct
    10981 tttctaatac ttcccaattc ccgatatgca aatcgagaaa ttgaggcaag cgttccatat
    11041 acatatcaaa atagtatttt gggggattta atggagcatg tggtttttca aaagacattt
    11101 tgagaaaaaa aggaaccgtt ggatctcttc tttgtaaaaa tgaaatggac tcactcacaa
    11161 cccagtttgt tggatgtaac ttctcatctt tatcccaagg tctagcttcc caagaattac
    11221 aatccattcc gtcatcaatc aaatcaacat cataaccaac tttacccttt aaaaaggcta
    11281 gatagtcact aacataatca aattgacttc cataagcctt atcatacttt ctatcaacat
    11341 gtagataccc atcgtgaagt aagacatgat caaacccaag tctctgtctg gaaggaaaaa
    11401 catgcatctt accaatacac tctgtttgat aacccatatc tttaaagact tttggaagag
    11461 tattagtaaa attccaaggc acctcatctt gataaccaac acgtccactt ttatcttggt
    11521 ctaagccagt caataaagct gccctagcag gaacgcaaga aggaactgga gaataggcat
    11581 tttcaaaatt ataacctaca ctagccatca tatctaatgt tggagtgcta actagcttat
    11641 ccttactatt caaggataag gcgtctgctc tcatttgatc tacaacaatc aaaataatat
    11701 ttggctgttt tgtctgaacc ataaaatctc ctttctaata tggcaaaaga ggcacaagaa
    11761 gatatctacc tttactgcac ccctttctat atcaatctct ctatataaag caataacatt
    11821 cttgttatgt tttatagaac aatgaactaa aatatgacta aatcgattag gaaattcaaa
    11881 tcattttcta gtactgtttt agaagttaca gtgtactatt ccaacttcaa caaactataa
    11941 accttggtct aataacaatt ttagtggaga taagaaatcc tacacctaac tcatcttaca
    12001 cgtaatctat ttctatttta tcacaaaaaa cgcaagtaag accattaact caattcagtt
    12061 ttatctgcca ttttcacaaa tgggaaataa gtcaagacac taataatcaa acaaacaact
    12121 gataagatga tggcacgcca atcaaatgct gtagagaaga aaccatataa aattggaggc
    12181 attacccaag taacattttg tgtaacaggt gaaacaagac cccagcttgt tgcccagtaa
    12241 gctaccgttg ccatgaaaac cgggctaagt acaaatggta taaatagcaa aggattcaag
    12301 acaactggta aaccataatt gaccggctca ccaatattaa acagaactgg tgctagacca
    12361 agtttagcaa cttttcgata atgactgttt cttgaaaaaa ttaaaatagc aagtaccaat
    12421 cctaatcctc caaaccagac aaacgcccca aaagacccac ttgtccatat ataaggaatc
    12481 ggttcacctt tttggaaagc atccagattc gctaacatag caactccaaa cagcccttcc
    12541 atgatgggag ccaatacatt tcctccatgg agaccaaaaa accagaataa cttatttaaa
    12601 aagatcatca gaataactgc aaagaaactt tgagacaaac ctagtaatgg cgtttgtaac
    12661 accttgtaaa cccaatcaat caataagtca ttgctaagta aatggaaaac ataagtcaag
    12721 atggctacta tatacatcgc cataaatcct ggaatgatag aagtgaacgg cttagcaatc
    12781 gcagggggaa ctgaatctgg taacttgatt acccagttct ttttcattac tttacagaaa
    12841 ataatagagg ctaaaaatcc aatcatcatg gctgtaaagt agcctctggc attaatatgg
    12901 tttcctggaa tcacattccc aatagttacc atcagatttt taccatcaaa tgctagatta
    12961 tcaattcctt gtaagatctg atctaatttc acatctccta catttgccaa agggaaactc
    13021 tttgtaactg tacttccaat cgaaatgaca aacgaagcaa gtgataccaa accagcagaa
    13081 actgtatcaa ccttgtaaat cttagcgata ttcactccca agcaatagat gaacaacaaa
    13141 gaaacaattg gtatacttcc cttgaatacc aaattattga tgtcaacaag ccactgaaag
    13201 gttttcgtaa tactttctag gtgaaattgt tgtggtaaat ccactagaaa agcatttaat
    13261 aacaaagcaa tggaacctgt cataataaca ggcatagtcc ccacaaatga atcacgtaaa
    13321 gcaactaaaa acttctgatt cccaactttc attgcaattg gagaaagttt ttcttgcatt
    13381 ttatccaaca ttgtattcat actaaactcc taattttttt caaatcgttc gtatacttct
    13441 accacttctt tcgccaagtc aacaaaagtc agactcgtca ttaaatgatc ttgcgcgtgt
    13501 accatcaaga ggctaattga tgtttcttcc ccctgtgcct ctcttgtcaa catttctgtt
    13561 tgagccttat gcgcctgtaa taaggcagca ttcgcatcag ctaatcttcg attggcttta
    13621 gagaaatccc ctttttttgc tgcctgaata gcctccatag cattactttt ggcttcccca
    13681 ccatacataa ttagccccat tacagattct aaattacttt catccataac attctcctca
    13741 atcttagtct aataaacttt cagctaaatc taaaacttta gaaccattca tcattccgta
    13801 atctgtcatc ggaataaccg ccacaggaat ctgtctatct tttagttttt cttgaaagtc
    13861 ccctagtaaa tagcgaactt gggggcctaa aagcaacaca tttacttctt ttgttgctac
    13921 aatttcttct gcttcaggag ctggaactgc aaaaatttct gcatccaacc ccttatcttc
    13981 tgctgccttt tgcatctttg tcactagcag acttgtactc atacctgctg cacacgctaa
    14041 cataattgtt actttagcca tagcttactc cttttatttt attgtcaatg ttactgtttt
    14101 tcgatcaact ttagactcat aatctaaatg tttgggacga ctagtataat gcaaattata
    14161 aactcctgga ggcatttgtg aaatcttttc tttttcagtt ttattgcgaa ttaataaatc
    14221 taccgaagta ttccctccaa agttattaaa cataggttca ttttcaatag aataatctaa
    14281 ctcatatcca ctattagttg taattccttt taataaatca ggaacatctc ctctgtttat
    14341 tactatatcc ttagcgccat ttattttggg agattgacta taatactctt cagcttgatg
    14401 ttgttgtaaa tcatttctag agtaagaaac ataatcaact acaaattcag tccctgataa
    14461 attattgtcc actaccccag gccaacttcc tcccatcgct aaatttaact gaacatactg
    14521 aggtctatta aaaatctttt tcgctatatt atctgatcca taatatactt ttctaacaat
    14581 atgattgtca accatccata caatataatc tttataccaa tcaattgaaa aagtatgata
    14641 cgaatcatta aagtttccac tcgataattt aaaggctgta ccacgtcctg caaattttcc
    14701 atcatcatta tccgactgtc cataatgaag cgtttgataa actgtcttat tcccacttcc
    14761 atttgaattt cgttcaccaa ctaattccat tatatctatc tcacctgttg acggccatcc
    14821 tcgtccctga acaggattaa tcttcccatc aagtgtaaaa tccgatccaa gagtccagaa
    14881 agcaggaaaa actccttgac ctttcggtaa ttttgcacgc atctctaatt taccataaag
    14941 aaattctact ttcccatgtg ttctaacact tcctgaatta tatacaacct ttctgtgatt
    15001 gtttcttggg ttaaaatact gatcttcctt agagcgattc gtagctctaa gaactaaatt
    15061 tcccgaacgt agaaaaacat tctcatcact tcttacataa tgttgctgtt cccaaccacg
    15121 aattgatcct aattcatatc cccaatattt tttatttaat tgttcgccat caaaatcatc
    15181 tctccaaatt ttcttatatg aatttccttt agacagttga tacatctcta cattatcaat
    15241 aaaaatatta gatttttttg tagtatcgtc ttctgaccat ttcactaatt gaatcaaaac
    15301 tttatcgtgt ttaccggaat tgaaagaaaa catgtatttt ttccattcgg ttgtattaac
    15361 tgtttgatca acagcttctc caatgttatt ttgaacaatc tctccttgct tattattatc
    15421 aaaccatctt gcggtaaaat atccctctgc tccactaata tctactttgg caaaagcaga
    15481 tactaaataa tcttcattag gttctatatc aataacttgt ccaatatatc catctgctct
    15541 attatcatta attgcagtag aaggaatcat acctgagtaa tgtccgttac tataactctc
    15601 atttgaatta attattcccc tatttctaga gttccaaaac agctctcctt gctcaaaatt
    15661 tgaatttttt attaaatttt cattagcaaa aactttcaca tgaataaata ttaataaaaa
    15721 taatataaat aaacttgaaa ttaatttctt attcttcatt ttatcaccta tttactgacc
    15781 tagccaagcg agatactgta tgtctcactc ctctaattgc cttgctcaat gcataaatat
    15841 tatcgactgt tgccagtccc ccatatcctg cgtcacctat atgttgaata tcaactccac
    15901 aaattttatt tctaagtgca atttccttga tagtatctgt atcagatgtt tcttggctag
    15961 taccaatagc actcagtact agcccaccct tactatgaac gagatcaacg acttcacgca
    16021 actcttggtc atgaaaggct ggaacggttc caactgctgg aacaagtatc acatccgcac
    16081 ctgcttccaa caattgctct gcgacagaaa gctctgccac aggctcattc actcctgccc
    16141 cgtgcatctt acctgcaata atcaaaccag aaaagttttc cttagcagtt tgaacagcct
    16201 taatgatttc tcgattgcta actcctgttc caggatttcc agtcaaacag acaaagtcaa
    16261 aacctaactc ctctattcga ttcaatgttt caacactagc aacacgacct gcaacaattt
    16321 cctgtgtttc ctctagcatc tttgcagatg ggtcaatcgg ttccaaattt acaccaattg
    16381 gacaagcaac aagctggtgt aagcgatgaa taacatcatc acctgaacta tccaaagcat
    16441 aaattttagg ctcaaaaaca tcaacacaat tcaataaaat catatcagct ccaaaagctc
    16501 tagcaatttc agaattagtt atatccccaa caaatgtctc acgagtaact acattttcag
    16561 atagaacaac tctaccttca cttgctaaaa tactttgttt taattcttca gcattcatct
    16621 gtaatatctc agatggattt gcactaatta aacgtttcat tctcaatcct ccaataactt
    16681 aaaccgtttt tcaatagcta ttacaaatgg taaataaagt aagacatcca aaacaatcaa
    16741 tacaatttgt aataatgaac cagaaatact tcctgtagca agtaaaccac ttataacagg
    16801 tggcatagtc caacttggat ctgtataagt tagcggaacc aaacctgatg ccattgctcc
    16861 ccacgctact agtaagttaa acattggagc taagataaat ggaactagta acaaaatgtt
    16921 caaaacaata ggaacgccaa acattgctgg ttcatttata ttaaataatc caggtataac
    16981 agttataggt gccaaagttt ttagttgttt actagctttc ttcttatgtg ctaaatatcc
    17041 taacgcaaga actaaaccta tggttgcacc accgccacct atgaatacaa aattatccat
    17101 aaatacgcta gtaaaaatat ttggaagagt ttctccagtt tgataagcta ctttatttgc
    17161 atctagatta gctaaccata acggtttcat aactgcatta acaacatttc ctccgtgtaa
    17221 tcctacaaac caaaaactac tattaaggaa aattaagata agaagaccaa taacattatt
    17281 tcctaaaagt ccaagtggac ctcccaaagc tccttgagca atttcatgaa gattaggtaa
    17341 acttaattta tctaaagttg cataaacaat caaccaacca acaatagtca ctgcaccagg
    17401 aataatggca ctaaagcttt tagatactgc tggtggaaca ccatccggca tttttatctg
    17461 tatattatga tttataaacc attggtagat atagccatta atcaatccta aaatcatagc
    17521 aacaaataaa ccttttgatg ccatgaaagc ggtaggcatt cctgctccag cctctcctgt
    17581 aatgaaaggc gtaactgtaa taaaagatga taatgacaca ataccagcag gaataccatc
    17641 aactttgtat tgtctagcca taaagtaagc gataccaaaa ctagctacaa gaccaagtaa
    17701 tccaaaacta ctatcaacac ccttccataa atagtcagca acaccaatat cacctagata
    17761 tttttcccat cctggaatag gaaaacttgc aataaccatt agtaatgaac ctatcaacaa
    17821 taaaggtata gttagagtaa taccgtctcg aatagaaacc aacgctttat tattacccaa
    17881 tctagccgct actggaagta acttttcttg taataatttt tccataacct tttcctctta
    17941 aatatatttt attatcaaaa gttatcaatt atctttattt tttgacgtca agttattatt
    18001 tgattatctt ttgttattta tattttacaa ctataagaaa gccttgtcaa ggatttgtgt
    18061 aaatatttga ttatttttta ttatcaatat ataataaaaa gagaccagta agcaatgaaa
    18121 agcttaaaaa gattaacatc tacatttatc catagcaaaa aatcatagga ttttaagaat
    18181 ctgacaacta ggaagaaaac taatatgacc ttatctaaga aacaattaca actaagagct
    18241 aaaatactgg aaacagttta cacattaggt cccatttcac gaattgaaat cgctactaaa
    18301 acaggaataa cacctgcaac aactagcagt atcacaaatg atctgattaa agaaaatatt
    18361 ctcttagaac ttggtgaaga cgaacacgat acaagcgtag ggagaaaaaa aatcctgcta
    18421 gacattcaag ctaaacgatt ttactacatt ggttgtgaac tatctgaaaa acatttcact
    18481 tttgctttag gagataatct aggaaatatc ttaaaggaag agaaagaaat tgttactaaa
    18541 cagttaatcc aagaaaaggg aaatcaactc attaaccaga cactaaagca attcttaaat
    18601 aactgctctg attatgaaat agaagcaatt ggaatcgcgc tacctggtcg ctatttagat
    18661 gattataaaa taacaacaaa caatccttta tggcaacata tcgacttaga aatgattcaa
    18721 tcccactttg ataagccttt gtttttctct aacaatgtta actgtatggc tataggtaaa
    18781 cgcctattta gtcgccaaca gaatgaccct aattttgctt attttcattt tgctagaggt
    18841 atgcactgtt cttatatcta cgacggaaac atttatggga aaggaaatct aatgattgga
    18901 gaaatagggc atacagttgt ttcatctgag ggagaagaat gtagctgcgg gcgcaaggga
    18961 tgcttacaaa cttttgctgg agaatcttgg ttaatcaaaa aatcaaaaat tctatatcat
    19021 caatctccct attctctact tcctagttta gtcaaaaatg ccgatgatat tgatatccaa
    19081 gttattttaa ccgcttatca attaggtgac actggaataa tcacacttat ccatcaagcc
    19141 ttactctatc ttagtcaaac cattttaaat atcagtatga tgattgactc tcaaaaaatc
    19201 tacctacata gtcctttact aacaaatcaa cacatcatcc aaaaactata tagtgaaatg
    19261 aactacaaac ccaaactgct ctataatcgc ctacctgaag tcattattga accttacaat
    19321 gattttacag ccgcacactc agctattgca ctttgtttgt atcatacaat tctacattct
    19381 taattttcta ttacatttaa taaataggct aaaaagaggg agtatatcat aaaaagcgaa
    19441 taaacctgtt ttctgaaaat cagaattctg ttttgttcgc tttctttata tttggaatta
    19501 tactcctatc tccccttttt cataatgtat agtgaaatga aataataaca ggacgaatcg
    19561 atcgggacag tcaaatcgat ttctaacaat gttttagaaa tagaggtgta ctattctagt
    19621 ttcaatccac tatattttcg tacaggtgct tcaaccattt gaacgatttc aaatccttct
    19681 ttttggtaaa gattctgagc tctttgattt gcctcgaaga catttagaga aatactatct
    19741 atatcttcat tttcaaaggc caaactaaca aatttcctta aagcctggct acctaagtct
    19801 tgctcctgtt tctgggggtt gataaaaaat ctcccgatat gaagattgct gtcttctagc
    19861 ctgattttct ggataagtcc cacaaactct tgttcatcaa agattgaaaa gactccttcc
    19921 aaggcttgaa ggggcagtag aaaaggaatc cttggtccca tccattgttc ttgaaaggat
    19981 ttgcctaggg agttggacca ctggcataca aattgagcgt tttctatact caccttttct
    20041 tcaaaacgaa ttgtcatctt ttcctcacca tcttatctat gtttctccat tatactattt
    20101 ctcccatttt ttacgaatag ataagtatga ttgattttta tttttttctc gtcgggagca
    20161 ttctcgcttc ctttcttggt ttggtcattg accgttttcc agagcaatcc attatcagtt
    20221 cagccagtca ctgcgattcc tgtcagactc ccttgcgtcc cttagatttg attccgattc
    20281 tctcacaggt cttcaatcgc tttcgctgtc gctactgcaa agttcgctat cctgtctggt
    20341 atgccctctt tgaactaggc ttaggactcc tctttctgct ttactcttgg gaattgcttt
    20401 ccttggggca agtcgtccta atcaccgctg gtttgacctt gggtatatac gactttcacc
    20461 atcaggaata tcccttactg gtctggatga ctttccacct aatcctaata gcttcctctg
    20521 gctggaatct ggtcatggtc tccttcctca tacttggaat tttggctcat tttattgata
    20581 tccgcatggg tgcaggggat ttcctctttt tagcttcttg tgctctcgtc tttagcgtaa
    20641 cggagttact gatcttgatt cagttcgctt ctgcgacggg tatcctggcc tttctcctgc
    20701 aaaagaaaaa ggaaagactt cctttcgtgc ctttcctctt acttgctact tgtgtgatta
    20761 tttttggtaa gctactactt gtttgataaa ttccaatttc tgccatatgt actccatgaa
    20821 attatttcac agttaaatta taaattattt cttttgtaca aagggatgat gttatcaaat
    20881 cgatctgttc ttctatcttc ttgatactga tcaaaaaatt tcatttcgac tgaaaatatt
    20941 tcgcttataa actgtaaacg aatactttgt ttagacatta tagtcgctag actgactaga
    21001 tgattactca aaacgacgtc cagaatactc tttactttgc ttggtttttt aacaaaaatt
    21061 tgatcatcca aaggttcaat cattttgtaa cctttttgcg caatttgacg ataaaagtaa
    21121 gaatgttgct ttggagtcaa taatcctaac ttaaaagctc gatactctaa agcctgtatc
    21181 gaaacattca aatccgactt caataaaata taactatcag gattgctgac acgcttgcca
    21241 accctctctt caaatttgac taaaaactct tcttttggca ataaaaaaca tgatgcaaaa
    21301 taatttgctt cttgctccaa acgatcgcca tcttcattca tatctttata tttatgtaaa
    21361 agaatatgtc ctagctcatg agctaaatca aaatttcgac gtacagatga tttattcgtt
    21421 cccaacacaa tataaggtct tcccaatttt gaccatgcgc tataagcatc agcttggcca
    21481 ttaattaatc gttccacgat atagatgcct gaacgttcta atttataaag caaatcatga
    21541 ttatcttttg aaatacctaa tttttccctg gcataaagag ccaattcctc aatggattct
    21601 cccttatgat aagattcact cactacatta cttaggtcat gaattgtaat attaggtata
    21661 attacaaaac tttcaaaata atcaatcaaa ctatctacct tatgtaaata catagtttga
    21721 atatctattg ttttccgggt tgctaggtct gcatttctaa aggcaattac agaagaatca
    21781 aatcgaatgc tctcttcttc ctgttcaaaa taagttaaat caacatgaaa ttggttggcc
    21841 aaatgcattt tggttgataa tttaggtttc gtttcgttgg actcaaactg ccaaatggct
    21901 tgttccgtta aattaattct ctgagctaat tctgctctac ttaaaccatt taacagccgt
    21961 aattctttca atacccgacc attaaacatt tacatactcc ttactacttt tgaccttctt
    22021 gtttttctat tcttggaata atttcaaaat cttctgtttc cgataattct gaaaaattag
    22081 gaatatcttg atatttagct tcttcgaaat ggtacgaact tgattgaata tactcagaca
    22141 aatcttgaat caagtgtaac tgaccagttc gtgcatcagg cataacaaca tctacagata
    22201 taatattgtt ttctgagtcc gcctcataag ttaaaatcat aaatttttcg atattcgaat
    22261 ttttagtagc ttgttcaatt tcttgaatca tttcatcaga aactaactcc atctgaattg
    22321 gaaaggaatg actatcttca tcatttttgt aggaagaatg ttgattaaga taaagtgtat
    22381 tcatctgagc atattcaaat aagtagccac tcttattttt ttgtaccaaa ggaaattggt
    22441 tcgtaagtcg cttcttaccc tttataatta acaatacttt cccatatttt tctgtgtttg
    22501 tttcaaattc taaatatccc caagtctgtc ctgctaattg taatttatac tcaaacaaat
    22561 ctgctgatgc aaatgcagta tcaatatgat taggtcgcgt ccatgcataa ccattcgaca
    22621 ctatcattgt ctctcttttt tctagacgtt catctacata atctttttgc cctttcatca
    22681 aagtatctac aattttttgt gcctcaagcg aatcaaagag atcctgattc aacataattc
    22741 ttcctcctcc aaatactttt taatgaatta tatcattttc ttaaagaaat tactacaata
    22801 attagctttt tcttaaagtt ctgtgtcaga gtaatttaga aaattatatc ttctatagta
    22861 aaatcaatta aaaactgaac aaatttattg ggaaattcaa atcactttct aaaaatattt
    22921 taggaaccgt agtgtaatat tccagattca atttactata aaactagcct ttctcctgca
    22981 aaagaaaaag gaaagacttc ctttcgtgcc tttcctctta cttgctactt gtttaattat
    23041 ttttggtaag ctactgcttg tttgataaag tcctgaatcg gctctccttg gtggagagct
    23101 tttactattt tcgaaccgac gataacacca tctgacaccg cattgaagcg ttccagatcg
    23161 gcttgactag atacaccaaa acctgtcaag actgggatgt cggccacttg atgaagttgc
    23221 gccaagtgct tgtccaaatc tgcacggtaa ttgcctgatt tccctgttac cccattgatg
    23281 gcaacggcat agacgaatcc ctccgcccct tcaatcaact ctttctggcg ctcaattcct
    23341 gtggtcaagc ttactaaagg aatcaaggcg atatctgtat ctgccaaaaa tggttctaca
    23401 aagttggcat gttcatgagg caggtctggg ataatcaagc ccttcacagc tgtatcagcc
    23461 agatctttga caaagttctc cacaccgtac tgaaagaggg ggttgaagta ggtcatgatg
    23521 accagtggaa tctctgtttc aatggttttc aaggtttcaa ctaaagcctg ggtagaggtc
    23581 ccgtgggcta aactgcgcaa gccagcttct tcgataacag gtccatctgc aacagggtct
    23641 gaaaagggaa tacccacttc aattgcagag acacccaaat cttctaaaaa gtgaattgtt
    23701 tcagcaagac catccaaacc tttttcgtgg tcaccagcca tgatataggg aacaaaaatt
    23761 ccttttccag ctgctttaat agcatttaat ttttttgtta gtgtcttagg catgagcttc
    23821 tcccttcttt gctgcatctg cttccaagcg gtcttttact tgaaccacat ccttgtcccc
    23881 acgacctgat aggcatacaa tcatagactt ttctggtcca agttctttgg ccaatttcac
    23941 cgcaaaggcg atagcatggc tagattccaa agctgggata atcccttcca cacgagacaa
    24001 gagttggaat ccttccaagg cttcttcgtc tgtcacaggg acatagctgg cacgtttaat
    24061 atcgtggtag tgagaatgct ctggaccgat accaggatag tccaaacctg ctgagataga
    24121 gaaggcttca agaatttgac catgggcatc ttggagcaca tccatgaggg aaccgtgaag
    24181 gacacctgga cgacccttgg tcaaggtagc tgcgtggtgc tccgtatcca caccaaatcc
    24241 agccgcttca gctccataca tggctactga ctcatcttct acaaagggat ggaagagacc
    24301 gatggcattc gacccaccac caacacaggc tactagggca tctggcagat tttgtcctgt
    24361 caagtcacgg tactgttgtt tagcctctcg accaatgaca ctttggaagt cacgaacaat
    24421 ttctggaaat ggatgaggcc ccaaggcaga accaaggata tagtgggtat catctatatt
    24481 agccacccat gaacgaaggg ctgcattgac cgcatccttg agcacgcgcg aaccatctgt
    24541 cacggcctcg accttagctc ccaaaagctc catacggaag acattgaggg cttggcgttt
    24601 gacatcttcc tcacccatgt agatggtaca ttccatgtta aagagggccg cagcagttgc
    24661 agttgccaca ccgtgctgac cagcaccagt ttctgcgata attttctttt tacccatgcg
    24721 tttggcaagc caaacttgtc ccaaggcatt gttaatcttg tgggctcctg tatggttaag
    24781 gtcttcccgt ttgagataaa tcttggctcc gccgatatgc tgggtcaagt tttttgcgta
    24841 ataaagagga gtttcacgtc ctacgtactg gcgcaaaagc tggtttaatt cctcttggaa
    24901 acttgggtct gcctgacttt cacggtaggc cttctccaac tccaaaactg ctgtcatcaa
    24961 tgtttctggg acaaaacgtc cgccgaattt tccgtaaaat ccatctttat ttggttcctg
    25021 atatgccatg ctttaccctc tctataaatc ttctaatctt ttcatgatct ttttgtccat
    25081 ctgtctccac tccgctcgat acatctactg catagggagt aaaatgttga attgctttta
    25141 ctacattatc ttcattaagg ccacctgcga taaagaaggg ctgtgctagt ccagtcgtat
    25201 ccagttgacc ccaatcaaag gactgaccac ttcctgccac aggggcatca aagagtagat
    25261 aatctgcctg agaattgggg acatgcccat ttccatctac ctgcacagcc tgaatactgg
    25321 cacaaggcaa attctcaaat aaatcagctg ccacctgacc gtgaacttga accaagtcca
    25381 agccaacttt gtcaatcgct tccagcagtt ctacccgact tggtgaaaca aatactccaa
    25441 cctttttcac atctgcagga ataagctttg ccaactcagc tgcctcttct aaagtcacct
    25501 gtcttttact aggtgcaaag acaaaaccga tatagtcggc tcctgctgaa acggctgttt
    25561 ccaccgcttc tttggtcgat agtccacaaa ttttaacctt tgtcaatctg caactccttg
    25621 attctctggg ccacattttc tgcctgcata agagctgtcc ctaccaaaat tccgttaaag
    25681 tatggggcta gtcgttccgc atcctgccct gtgaaaatgg cagattcaga aatgtaatag
    25741 cgaccttcct caaagtaagg ggctaaatct acactggtct gcaagtcgac ctcaaaggta
    25801 gtcaagttgc ggttgttgac cccgataatc tcagcaccaa gtctgtgggc tacctctagt
    25861 tcagctagat tgtgagtctc cactaagact tccagaccaa gctctgtcgc gtagtcatac
    25921 agttccttga ggcgttcttc ggacaaggct gccacaataa gcaagataac tgtcgcacct
    25981 gtattgcgag cgcggatgat ttgcttttca tctatgataa agtctttgtt gagcgtcgga
    26041 atctctacct gactggaaat ttcccgtaga taatccaaat gccctttaaa gaaaacctca
    26101 tctgtcaaca ccgaaatcat cactgctccg ttttcttcat aagtctgggc ctgttgcaca
    26161 atatccacat cgagattgat atctcccaaa ctagggctag ctttcttgac ctcagcgatt
    26221 acctgcaagc agtcctgatg attcttcaaa aattctgcca agcgataggt ctggcgcaga
    26281 ggctggattt gctccagctt catctgctcc acctcacgcg ccttctgctc taagattcgt
    26341 gctaaaaatt cctgactcat ttttggtact cctgtaacag tctgagtttt tcaagggcct
    26401 tgcctctagc aatcacttga cgggccaagg caactccttc cttgatgcta tcaatcttac
    26461 cattagcata gaaaccaaga ccagcattca agactgtcgt ttccaagaat ggacttgctt
    26521 cgtttttcag aacgctaagc aaaatttctg cattttcctg agcattccca ccacgaatat
    26581 cttccatagc atagtcttcc attcccaaat cctctggagt aaagcttgac aagctgattt
    26641 cgccattttc aagaagtgca atcttggttg ttccgttcaa gccagcttca tccaaccctt
    26701 ctggtccagc aaccacgatg gcacgtttgc gacccatatt tttcaaaacc tgagctgtac
    26761 tttctaggag ttctggacga ctaattccaa gaagctgtgt ttctaaagcc attggatgaa
    26821 tcagtggacc agtcaagttc ataatcgttg gaattcccaa ttccaaacga gctggcatga
    26881 tgtatttcat agctgggtgc atatttttag cgaagagaaa gacgattcca gttttatcaa
    26941 agaccttacc tagttcagct ggtttgaggt caagattgat tcccaaggct tcgaggacat
    27001 ctgcggaacc agatttagaa gaaatcgagc ggttaccatg cttggccatg tgaataccgc
    27061 caccagccaa gacaaaggct gcagttgtgg aaatattaaa actgaaagac ttgtccccac
    27121 ctgtaccaca gttgtccata gcatcatgaa tctcagttgg aatatgctgg gcatgtcctc
    27181 tcatgacttg ggcaatggct gtacgttctt caggtgtttc ccccttcatc ttaagagcta
    27241 agaggagaga agcaatctgc gcttcagtta cacgcccagt tacgatacgc tcaatgacat
    27301 ccgtcatttc cacacctgat aaattttcaa attttgctag tttttcaata atctctttca
    27361 tcctagtttc ctcactttac aaccttctcg ataaaattcc gaatagaaga caagccgtct
    27421 ggcgttccaa tgctctctgg atggtactgg aagccataaa tcggtaggtt tttatgttga
    27481 atccccatga tggcttggtc atcagtcgaa cgagctgtca cttcaaagtc ttctggcatt
    27541 tcctcaatca aaatactgtg ataacgcatg accgcacgtc catcctcaat accttgatac
    27601 aaaacagatg gcgcttcaaa gttgatattg ctctgtttcc catgcatgac ttttggagcc
    27661 aaacctagct taccaccaaa gacttctgca atggcttggt gacccaaaca aatccctaga
    27721 atcggcttct tgcctgcaaa atcacaaatc atgtcttcca tctttccagc atcaactggc
    27781 caaccaggac caggagaaaa gaccagacca tctgcttttt cagcttcttc atacagcttg
    27841 gaatcatcat ttctcaaaac ctgaacttct gcaaaattcc caatgtattg ggccaagtta
    27901 taggtaaaag aatcatagtt gtcaatcaat aaaatcatgg tcttagttct ccaattctag
    27961 tcatagattt tgctttgtta atggtttctt ggtattcgtt ttgggcgata gagtcgtaga
    28021 caatccctgc cccagcctgc acataggctc tttgattttt gagaatcatg gttcggatgg
    28081 caatggccaa atccatatca cccgtcgcag acaagtagcc gattgctcct gcatatacgc
    28141 tccgtttttc cgtttccagt tcatagatgc gtctcatagc tcgaattttt ggtgctccag
    28201 aaacggttcc agcaggaagc gttgctttca aggcatccat ggcagtgagt tctgcaagca
    28261 aacgtccctt gaccacactg gtcaaatgca tgacgtagcg gaagagctcc acctccatat
    28321 acttagtaac ttggacactg gccgtttcag agatgcggcc aatatcgtta cgccccaagt
    28381 ctaccaacat tcgatgttct gctgtttcct tctcatcaga gaggaggtca gtcgccaagg
    28441 ccttgtcttc ttcatccgta gcccctcttg gtcgcgtccc tgcaatcgga ttggttgtca
    28501 cgatgccatt tttgacagaa accaaacttt ctggactagc tccgatgatt tgataatccc
    28561 caaaatcata gaaataaagg taattagatg gattagtcac gcggagattt ctgtagaagt
    28621 caaatggatt tccagtaact tctgctgaga aacgctggct gagtacacat tggaacatat
    28681 ctccgttacg aatcaagtca cgagctgttt ctaccattcc ctcaaactta tgtggagcga
    28741 tatgcggttt gaagtcaagt ggtgataaat ccaagtcttc aaattcattt ggagcaggaa
    28801 tgcgtaattc ctcaagcact tggttcaagg atttttccaa ggcctcttga ctgcgctcac
    28861 tataaagtgc atcctctatg acatgtatct tctccttctt gtggtcaaag accatatagc
    28921 tctcatagac aaagaaatgc atgtctggcg tcccaattgt atcctcaggg atttgaccaa
    28981 tttcttcata aagcgaaatc atatcgtaac caacaaaacc aatggctccc ccaccaaaag
    29041 ggaggtctga atggtgctgg ctcttatgag tcacttcata aaggaaatcc aagggatccc
    29101 gatcaatcgc ttgaccattt tgatagagaa ctccattttc aaacttaatc tcaaaaactg
    29161 gattataggc taggatagaa aaacgagctg tttccttgtc tctcggaata ctctctaaaa
    29221 taaccttatg ttgccccttt aagcgcatat aagccaagat tggtgataag acatctccat
    29281 gaatgattcg ttccattgtc atttcccttt cagttctaat tctagttcgt ggcgactgta
    29341 tgaaaaatcc ccacgcaaaa taacttgcgt gaggacgaaa ttcgcggtgc cacctcaatt
    29401 ataggatttc tcctatctct cattcctgtc tcagatatct cctgtaacag gctgtgcgat
    29461 aaagggcact cccttgagaa tgatgttttc ttctctcgtt tcagatgaac ccaactttac
    29521 agctttctct gcttgttttc agcaaccaca agctctctgt gagagaaaag actgtaattt
    29581 ttccatctat tattttttag cttctagtaa tctgcaatcg cagctaggtc cttgcctcca
    29641 cgaccagaga cattgatgaa gagatgttca tctcggtaca cctttatact cttcgaaaat
    29701 ctcttcaaac cgcgtcaacg tcgccttgcc gtaggtatgg ttactgactt cgtcagttct
    29761 atctgcaacc tcaaaacagt gttttgagct gacttcgtca gttctatcca caacctcaaa
    29821 acagtgtttt gagcaacctg cggctagttt cctagtttgc tctttgattt tcattgagta
    29881 ttactagctt ttttcgtatt agtccagcct ttttgtttac ttttagtagg aggcaaggag
    29941 ctgtagatag aactcaagtt catcaaagcg acttaaggcc ctaataaaag ataaaccaaa
    30001 cgacggatag aaaaaagccc acacacagaa tatacttccg tgtgagggcg ttggtaacgc
    30061 ggtgccacct caattataaa gggactatcc ctttacatct ctgccttgtt taacaacaag
    30121 ctgcactgta aggtgtgcgc accgaatttt cattgtttca aattcatttt caaaatcagc
    30181 ccactttcac tacttccaac cacctattca caatcaccac aggctccctg aagatcaaaa
    30241 atagttactt ttctgatttg ttgaacttat tttaatactt tgttttttct ttgtcaagac
    30301 ttttttacga tttttttgat atcattcgaa tgtgaccata tcttccttag atcgaacatg
    30361 aacatgtccc acttgttaga aattggatcc aactcaatag aaactgaatg gaggctaaac
    30421 agaacttatt ttagaacact ccatcttttc cactaggatt ttcaagaatt aaacaatact
    30481 agaaactctg tctcctaaca aatttaggag aaacttcaac agatgtgaca ctttcccctt
    30541 taataattgc taaaacacct tctatcattt ctttagccaa tttaacataa ttgggagcaa
    30601 ttgtagacaa agctggagta taatactgag aaataggaat attatcaaat ccaatgatag
    30661 aaatatcatc tggaataaga attcctttct catagcacgc acgaatcaag ccctgaacct
    30721 tttcatctcc tgaaacaaaa ataatgtccg gataattttg ggtagtcaag tgctgcattg
    30781 cataagaata aactgaatca attgtagata agccataaat gacttttaaa tccataaagt
    30841 aatttttatc attcagaaaa gaacgcacac ctctttcacg atccttatta acatgggatt
    30901 ctcctcccat aagcaaccac atatttttaa atttttcttc agttacagct ttcatcatat
    30961 cataagtagc ttgaaaatta ttattagata catagactac tccagacgtt tgagattcac
    31021 cgaaaacaag aaaaggcata tggttcttct ttaaatactg aattctgata tcatctacac
    31081 tttcataaaa aacaataaca ccatctacta ggctacctgt gcttgatata attgaattac
    31141 taattgtatc ctcctctcca aagtactcaa ctatagcatt aacaccaaat tctttacacg
    31201 tccgtaacac tttatctaac agcgtatgaa accaaattaa aggaaaagag tcgatttttt
    31261 ttacagaaat caatatattt atagcttctt ttttagttaa attttttgca tacgcatttg
    31321 gaatatacga caattcctct ataacttttt gaatcgcttg ataagtttct tctttaacat
    31381 ttactccacc attaataact cgtgaaactg tttttggaga aaaacctgat aaacgtgcaa
    31441 tatcataaat agttaccttt ttcccattta tatttttcat ttcagtcctc cattacgaac
    31501 attctaatat tactatacaa tatttaattt tttttaacaa gagaatttag taaattattt
    31561 aagatccaca aattcacaaa attaatttta caaatattct tccccttcaa aaaaatttaa
    31621 attgcatttc acacctttat ttttaagaat gtttccaact tcacgacaaa taaattcata
    31681 tgagaaaaaa ctgccataaa attgtagatt aactttttca gtaaaatgtg taggatttat
    31741 aaaaacatat aatagcctgt caatgtaaca ttttaacata gagttaattt tttctttaaa
    31801 gataacattt gttatcaact catcaggagg taaatgaaag gcaaacacca tttcacaaat
    31861 atcataaaaa gaaataaatt tgtatacttg tatcaaacaa ttattatcaa aatattctat
    31921 tttacctaaa tcaaaattga ttttataatc tttcataaaa acctctgagc aaaaatctac
    31981 tcaaaaatta gatgattaaa acatctaaaa aacaaaagga caaaaacatc tgtccctttg
    32041 tttactaaat ttcagctaat ttcttcgaca taaataacac ctacaatatt agcaatttct
    32101 tccatcagtc gaagatgttc aaatctacct gataattcca gagtaataaa tgacgctatt
    32161 tttttatctg gaacatcaaa gtattcaatt ctgtcagaat taacatctcc aaacgctgtt
    32221 cttgaatcgg tcattctgat accattttct gcacaataaa ccaatacacg attataggct
    32281 tctgtagatt taaccactat atacaattca atcattttag aacgattttg tagatatttt
    32341 tttagtggtt ggaacatgga tatcacaccc caaacagaaa tggctactaa aagagctccc
    32401 tcataaaaac ctactccaat agctaatcca attcctgccg aagcccaaat gcctgctgca
    32461 gttgtcagac ctgtaatttt ctttttatct gtaataagaa tcgttccagc gcctagaaaa
    32521 cccacacctg atataacttg agctcctaat cgtgtaggat ctcctgtccc aaatttataa
    32581 gatacgtatt cattcgtcat cataatcaaa catgcagcta gacaaacaat actataagtt
    32641 cggatgcctg caggctggga tttgctccct ctctctaaac caattatact accaatgact
    32701 actgataaaa caatcctgac aactatttca atatttgata acccaagact agtggctgtc
    32761 atgattattt ccttacttta cgccccggtc tttgtgtgaa gtataatacc gttccagaaa
    32821 taatcatcag aacaattgta taaacaaata ccagagcttg tgcattagat gttgctgttt
    32881 catcacctgc agatcgaatc gtaataccta atggttgagc taggggatgg taaaggaata
    32941 cagataagtc gaagtcagtt aataaagagt taaagtttaa agcaataaca gagagaacaa
    33001 ccggtaaaat aaatggaatg ataaccttca tcatagtata aaaaggtgaa gcacccatac
    33061 ttcttgctgc atcttccatc tcatcatcaa cactaaataa aatagcacgt accattctat
    33121 aagaaaatgg gatttttaca actatatatg caataagtag aattaccaaa ctacctacca
    33181 aaatctgatt caagacaaga aattgtggct gattaaaagt aaataataaa cttactgcta
    33241 aaagcgtact tggtaataac caaggaagta gagcaccata ttcaaataag aaatcaaaac
    33301 gagatttatg ttttctgaca acacgagcaa atacaactgc gagaattgtt gctgttgtcg
    33361 cagcaataat agaataaata aagctgacca agaatggaga gaatgccgca ctattactaa
    33421 agaataagcg ataattttct aaagtaaagt ttgataatgt taagttacct gtttgaattg
    33481 caactggatc tgtaaatgag tataatacta taaaaattag tggaagcatg aaaactgtga
    33541 acaatccata tgctacaatg tgagcaatga tattccaagg cttagacgca attttttgtt
    33601 ttttaagagg cgctttagtc ttagagatag aaatataatt tccacctttt tctatcttat
    33661 tcatgatagt aagcaaaatt gtagttgcaa tacctaaaat aattgcaagt aaggcagcta
    33721 aatcacgaga attccccatc cctgcaaatg taataatcat tggatttata gtttgaaatt
    33781 ctttaccacc aacaatcatg ggtgctgcta ctgcagataa accactaaga aaaaccataa
    33841 tagtaagtgc aaatagagtt ggaattaagg ttggtaacac tacttttcgg aaaacagtaa
    33901 atggttttgc tcccatattt cgagcagcct caatagtgtg atagtcaacg cttcgaattg
    33961 tatttgttaa aaacaatgta tgattagcag ttcctgaaaa tgtcataatg aataagactg
    34021 caccataccc aataaaccag ttagggtcta aagaagggat aacattttgt aaaaattttg
    34081 taatcaatcc atatggacca tagacaaatt tatatccagt cgctaaaacc actcctccat
    34141 aaattaaaga ggtcatataa cctaatttta aaattttagc acctttaata tcaaagtact
    34201 ctgtaaatag aacacaaaga atacctacga cattaactgt aataatgagt gaaaatgcta
    34261 acttaaaact gttcataata ctctgaagtg ccctctgaga ttttagaaca cgatgtacag
    34321 catcaaggga aaattctcct ccttttacaa atacattcac tactagatca aagtttggat
    34381 aaataataaa tgttactaag aaccagatta accctaaacg aataagccaa tcttttaaat
    34441 ttaatttatg acgcatactg cacctcctta aaattgcaga acgtctgatg gtgtgataaa
    34501 taattccaca ctttctccga cagatctaat agcagcctga ctatcaatac ttgttacatt
    34561 aagaatctga ctttcagaaa cttttattgt atagtgaatt gtaactccag aaaactcaac
    34621 atcaataatt gtccctttta gaatgaaatc ttgttcagtt tcacgattga atcgaacttt
    34681 ctctaatcga atgtatcctt ttttatcttc taagaaaacg cttgtatttt tcagtaatac
    34741 ttcgtggact gtttcatcgg tcaaaacatt aatatctcca ataaaatcac atacaaattc
    34801 agtttgagaa ttatgataaa tctctactgg tgtaccgacc tgttcgatgt atccattgtt
    34861 aaagactgca attctatcag ataaagtcaa ggcttcctct tgatcatgag taacatataa
    34921 agtagtaata cctaactctt tttgaagtct tttcaactct tttctcaaat ctacacgtaa
    34981 ttttgcgtca aggtttgaca atggttcatc tagacaaaga attttaggtt caagaaccag
    35041 agcacgagcc aatgctaccc tttgttgttg acccccagat aattctgata cattacgctg
    35101 taactgttga tcagagatct taatttttgc tgccactgct gatactttag ctttaataac
    35161 atctggagct accttcttaa cttttaaacc aaatgcaata ttatcaaaaa cagtcatagt
    35221 tggaaatagc gcataagatt gaaatacaat accaattcca cgcttttcag gttccaaatg
    35281 agtgacatct gttccattaa cttcaatact tcctgatgat ggatctaaaa aacctaccaa
    35341 tgctctcaaa gtagttgatt taccacatcc tgaaggccca agaaatgtaa agaattcccc
    35401 ttcatgtata tctaaattca gattatcaat tgcaacaaaa tcaccatatt taatttgaat
    35461 attatcaaat ttaatcatct cactaactcc ctctattact aaaccaaaag cctctcttta
    35521 tttcttcctt aaatttagaa ataatagaga gacttggaca taaaaaataa ctcttatttc
    35581 ttattttacg tattctaatt cagctttttc tacccattca tccaaatgct ttccaacagc
    35641 ttcccagtca atattttgtg gtttcacttg atcaacaaat tttttcgtat cttcaggtaa
    35701 ctcttttagg gcatctttat ttgcaggaat agatccaaat tttttactat attctacttg
    35761 aatttctgat tgaccaaacc aatcaataaa ttctttagct aacgcttgtt ttttactagt
    35821 gcttaaaacc atagtttgtt cagttacaaa tggtacacca atctcaggag tcataacttt
    35881 gaattcaaca ttttgttctt tttgtccgat taacgctcca gaaccccaga tcataccgta
    35941 ttgaattggg ctttctttat ctaacgcttt aacaactgag ctttcacctt tttgaagagt
    36001 ataggcattt gcaaagtatt cttttgcaac ttcccatcct ttttctgata cgcctaaatc
    36061 tcctttttca tcaaggtaac gaactaagat acttgctaga attgcccgtc ctgtaccacc
    36121 ttgaagacca taaattgaat atttaccttt atacttacta cctaattcag tccaatcttt
    36181 aggcatttct tttacatcag gcgccccaat taaaactaat ggttgaacaa tcacaggatt
    36241 ataataatta tctttatctg ataaagattg atcaatttta tctaaccatt taggcttgta
    36301 ctgtactagt aatttttgat ctctaatttt atttgagtca acagcaccaa ttccaaatac
    36361 catatctgca actgcattat tcttctcagc aataacacgg tctgctaatt gagcgccagc
    36421 aatatcaacc atttttatat taaaaccagc ttcttttgct ttagcagtta accaatcacc
    36481 acgaccattt gagactgagt tcgaatagat tactaattct tgacttttat cagctttttc
    36541 ttcagatgaa gaagcagtcg tagaatttga acctccagag caagcagcaa gtgtagtaag
    36601 agcaactccc gttgcaagta cagtagacca aactttcatt tttttcatga taagttctcc
    36661 ttttttatta ttttatttaa atttttcgtg atatggaaca aattgtctca tatcttcaaa
    36721 tacagtatag tcaatacggt ttacagtaat agttggaatc ttctctaata aaatttcagt
    36781 taattctgct ctgactttag taaactcttc ttcctcctct tcggttagag gaatccgaag
    36841 atacccaatt gaaatatgga attgatatct atcatgatta gggaaacaaa cacctgcttt
    36901 ttctgagaca taagtacgaa tttcttctaa tctctttgca gaagcttcat ctgcaggttc
    36961 aactagtatg ttttgttttc ccatttcagt tatacgcata tgaatttctt catccaacaa
    37021 tggaaaaatt tcaagttgtt tagcaaagta atcatgtatt tcctgtaaag gtgtatctag
    37081 aggaagatta ctgctccaaa actccgtttc acgattttca tggcacaaca attcaattac
    37141 agtcatgtga atagaattcc ttggagttaa agtaaactta tcgataaatg gtaattctct
    37201 ataacgtgat tgaataatat caacaacttc catcaaatct tgtttagtat aaagatttgc
    37261 tacaactgta ttcccaggga aatgattaaa ttccccattc tctttgaact taatttttgt
    37321 taagtttttc atataaagac cttctttttt atatttgaca tcggtgtcat tgtgtttaca
    37381 ttatacaatg aaaccgtttc ctttgtcaag cgctgtcaaa catttttttt aatttatctt
    37441 tcattaaaag
//
LOCUS       DAVBBS010000015        37265 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVBBS010000015 DAVBBS010000000
VERSION     DAVBBS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37265
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.015"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            215..1978
                     /gene="aspS"
                     /locus_tag="VQU32_000742"
     CDS             215..1978
                     /gene="aspS"
                     /locus_tag="VQU32_000742"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET2472226.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEKEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVVNATLGTLRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            1956..2897
                     /locus_tag="VQU32_000743"
     CDS             1956..2897
                     /locus_tag="VQU32_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET2472227.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(3017..3097)
                     /locus_tag="VQU32_000744"
                     /pseudo
     CDS             complement(3017..3097)
                     /locus_tag="VQU32_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(3233..4219)
                     /locus_tag="VQU32_000745"
     CDS             complement(3233..4219)
                     /locus_tag="VQU32_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2472228.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(4229..5029)
                     /locus_tag="VQU32_000746"
     CDS             complement(4229..5029)
                     /locus_tag="VQU32_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HET2472229.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRIYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(5290..6132)
                     /locus_tag="VQU32_000747"
     CDS             complement(5290..6132)
                     /locus_tag="VQU32_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2472230.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(6134..7426)
                     /locus_tag="VQU32_000748"
     CDS             complement(6134..7426)
                     /locus_tag="VQU32_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2472231.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGNKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(7537..8808)
                     /locus_tag="VQU32_000749"
     CDS             complement(7537..8808)
                     /locus_tag="VQU32_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2472232.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            9358..10875
                     /gene="malQ"
                     /locus_tag="VQU32_000750"
     CDS             9358..10875
                     /gene="malQ"
                     /locus_tag="VQU32_000750"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET2472233.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            10901..13159
                     /locus_tag="VQU32_000751"
     CDS             10901..13159
                     /locus_tag="VQU32_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HET2472234.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIDLDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(14203..14775)
                     /locus_tag="VQU32_000752"
     CDS             complement(14203..14775)
                     /locus_tag="VQU32_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472235.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAINLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(15397..16245)
                     /locus_tag="VQU32_000753"
     CDS             complement(15397..16245)
                     /locus_tag="VQU32_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET2472236.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            16390..16668
                     /locus_tag="VQU32_000754"
     CDS             16390..16668
                     /locus_tag="VQU32_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472237.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            16670..18733
                     /locus_tag="VQU32_000755"
     CDS             16670..18733
                     /locus_tag="VQU32_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET2472238.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(18811..20067)
                     /locus_tag="VQU32_000756"
     CDS             complement(18811..20067)
                     /locus_tag="VQU32_000756"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET2472239.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            20209..22674
                     /locus_tag="VQU32_000757"
     CDS             20209..22674
                     /locus_tag="VQU32_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET2472240.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            22739..23650
                     /locus_tag="VQU32_000758"
     CDS             22739..23650
                     /locus_tag="VQU32_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET2472241.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCLEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVSVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            23880..24578
                     /gene="dapD"
                     /locus_tag="VQU32_000759"
     CDS             23880..24578
                     /gene="dapD"
                     /locus_tag="VQU32_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HET2472242.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVCVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            24646..25776
                     /locus_tag="VQU32_000760"
     CDS             24646..25776
                     /locus_tag="VQU32_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HET2472243.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFTSQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            25788..26327
                     /locus_tag="VQU32_000761"
     CDS             25788..26327
                     /locus_tag="VQU32_000761"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET2472244.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLTPKTYPKGRMDFVVYDPQQLVKTSFALLEPQ
                     GDLEVVDASQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGRALSTIYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            26311..26988
                     /locus_tag="VQU32_000762"
     CDS             26311..26988
                     /locus_tag="VQU32_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET2472245.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(27060..27959)
                     /gene="galU"
                     /locus_tag="VQU32_000763"
     CDS             complement(27060..27959)
                     /gene="galU"
                     /locus_tag="VQU32_000763"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HET2472246.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(27981..28997)
                     /locus_tag="VQU32_000764"
     CDS             complement(27981..28997)
                     /locus_tag="VQU32_000764"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET2472247.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     YHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKAI
                     IMHASKGLEPDSHKRLSTILEEEIPEQLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            29209..29586
                     /locus_tag="VQU32_000765"
                     /pseudo
     CDS             29209..29586
                     /locus_tag="VQU32_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(29766..30191)
                     /locus_tag="VQU32_000766"
     CDS             complement(29766..30191)
                     /locus_tag="VQU32_000766"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2472248.1"
                     /translation="MNNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKSLTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(30259..30909)
                     /gene="phoU"
                     /locus_tag="VQU32_000767"
     CDS             complement(30259..30909)
                     /gene="phoU"
                     /locus_tag="VQU32_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET2472249.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(30924..31676)
                     /gene="pstB"
                     /locus_tag="VQU32_000768"
     CDS             complement(30924..31676)
                     /gene="pstB"
                     /locus_tag="VQU32_000768"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2472250.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDVLLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(31678..32493)
                     /gene="pstA"
                     /locus_tag="VQU32_000769"
     CDS             complement(31678..32493)
                     /gene="pstA"
                     /locus_tag="VQU32_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET2472251.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(32486..33349)
                     /gene="pstC"
                     /locus_tag="VQU32_000770"
     CDS             complement(32486..33349)
                     /gene="pstC"
                     /locus_tag="VQU32_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET2472252.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRTFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(33467..34342)
                     /locus_tag="VQU32_000771"
     CDS             complement(33467..34342)
                     /locus_tag="VQU32_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HET2472253.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(34509..35840)
                     /locus_tag="VQU32_000772"
     CDS             complement(34509..35840)
                     /locus_tag="VQU32_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HET2472254.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSALKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(35841..36548)
                     /locus_tag="VQU32_000773"
     CDS             complement(35841..36548)
                     /locus_tag="VQU32_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2472255.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(36632..37117)
                     /locus_tag="VQU32_000774"
     CDS             complement(36632..37117)
                     /locus_tag="VQU32_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET2472256.1"
                     /translation="MAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            37103..37265
                     /locus_tag="VQU32_000775"
                     /pseudo
     CDS             37103..37265
                     /locus_tag="VQU32_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT        10926 a         7066 c         8037 g        11236 t
ORIGIN      
        1 ggttttgagg ttgtagatga aactgacgaa gtcagtaaca atacctacgg taaggcgacg
       61 ctgacgtggt ttgaagagat tttcgaagag tattagcgct aatttttagg gctttcacct
      121 gacttttaaa gactggcaaa cttttctgat gattttcatg aagagcctgc gcttttatgg
      181 taaaatagta acagaataaa agaggagaga aataatgaaa cgtagtatgt atgctggtcg
      241 tgttcgtgag gaacacatcg gacaagaaat aaccttgaaa ggatgggttg gccgtcgtcg
      301 tgaccttggt ggtttgatct ttatcgatct tcgtgaccgt gaaggaatca tgcagttggt
      361 tatcaaccct gaaaaagtat ctgcagaggt tatggcaaca gctgaaagcc ttcgtagcga
      421 atttgttatt gaggtgactg gtcaggtcgc tgcgcgtgag caagccaatg ataagttgcc
      481 aactggtgcg gttgagttaa acgtgacagc tcttattgtg cttaacacag ctaagacaac
      541 accatttgag attaaggatg gcattgaggc aaatgacgat acacgtttgc gttaccgtta
      601 ccttgacctt cgtcgtccag aaatgttgga aaatcttaaa cttcgtgcca aggtgaccca
      661 ctctatccgc aactatttgg atgagttgga gtttatcgac gtggagacac cattcctttc
      721 taagtcaacg cctgaagggg cgcgtgatta tttagtgccg tctcgtgtta ataaggggca
      781 tttttacgct cttcctcaaa gtccacaaat cacgaaacag ctcttgatga atgctggttt
      841 tgaccgttac taccaaatcg ttaaatgttt ccgtgacgag gacttgcgtg gagaccgcca
      901 gcctgaattt actcaggtcg acttggaaac gtccttcctt actgagaaag aaatccaaga
      961 tattacagaa ggcttgatcg cgcgcgtgat gaaggaaaca aaaggcatcg aagtaacgct
     1021 accgttccct cgtatgaaat acgatgatgc tatggctctt tacggttctg acaagccaga
     1081 tactcgtttt gacatgttgc ttcaggactt gacagaagtg gtcaaaggtg tagactttaa
     1141 agtcttttca gaagcacctg ctgtaaaagc gattgtggtc aaaggagctg cggacaacta
     1201 ttcacgtaaa gacatcgaca agatgacgga agtagccaaa cagtatggtg ccaaaggtct
     1261 tgcttgggtc aaggtggttg atggagaatt aaacggacca gttgccaagt tcttgactgg
     1321 tatccaagaa gaattgacaa cagcgcttgc tcttgaagat aaggacttgg ttctctttgt
     1381 ggcggatacg cttgaagtgg ttaatgcaac actgggtacc cttcgtggac gtattgctaa
     1441 agagcttggc ttgattgata atgataagtt caacttcctt tgggtggttg actggccgat
     1501 gtttgaatgg tctgaagaag aaggccgcta catgagcgcc caccatccat tcacacttcc
     1561 acaggaagag actgctcacg aattagaagg tgatttggct aaggttcgtg ccattgctta
     1621 cgatatcgtc ttgaacggtt atgagcttgg tggtggtagc cttcgtatca accaaaaaga
     1681 ccttcaagaa cgcatgttca aggctcttgg tttctcaact gaagaagcca atgaccagtt
     1741 tggtttcctt cttgaagcca tggactatgg tttcccaccg cacggtggtt tggctatcgg
     1801 gcttgaccgt tttgtcatgt tgcttgctgg agaagaaaat atccgtgaag tcattgcctt
     1861 tcctaagaac aacaaggcaa ctgacccaat gacacaagct ccatcaacag tcgctctcaa
     1921 acaactagag gaactcagct tacaagtaga agaagatgaa acaaacaaaa cgaattaagc
     1981 ggtggcgcta ttatctgcgc cgctttgctt atcagataaa aattttacgt gtcttacaaa
     2041 gtatctctcg agaaaagtat gatgagaaga tttcggcctc tctggtctat ggttttttat
     2101 cagcagtagc agttaatttc tttttccaac cagggcatgt gtattcgagt ggtgcaacag
     2161 gtctggcaca gattatctct gccttgagta atcactggtt tggttttcat attccgattt
     2221 cgctaagctt ctacgccatt aacttccctt tgatggtctt agcttggtat cagattggcc
     2281 ataagttcac cgtctttacc tttatcacgg tatctatgag ttccttcttt atccagtttg
     2341 tccctgtggc aaccttgacg gaggatccca ttatcaattc cctttttggg ggtgttgtta
     2401 tgggtttggg gattggtttt gctcttcgaa acaatatctc cagtggtggg acggatatcg
     2461 tcagcctgac tattcgtaag aaaacgggta agaatgtcgg tagtatttct ttcttggtaa
     2521 atggaactat catgctgata gcaggtttga cctttggttg gaaatacgct ctttattcta
     2581 tgattaccat ctttgtctct agccgtgtga cagacgcagt ctttactaag caaaagcgta
     2641 tgcaggccat gattgtgaca aatcatccag agaaggtaat tgaaaaaatc cataaaaaat
     2701 tgcaccgcgg agcaaccatg atccacgatg cagaaggaac ctataatcac gagagaaagg
     2761 cagttttaat cactgtcatt acacgtgcag agtttaatga atttaaacag attatgacac
     2821 aagtggatcc aagctccttt gtctctgtct cggaaaatgt tcatattcta ggaagatttg
     2881 ttgagataga taattagtga ttaatactct tcgaaaatct cttcaaacca cgtcagcttc
     2941 accttgccgt atatatgtta ctgacttcgt cagttctatc tgcaacctca aaaccatgtt
     3001 ttgagctgac ttcgtcagtt tcatctacaa cctcaaaacc atgttttgag caacctgcgg
     3061 ctagcttcct agtttgcact ttgattttca ttgagtataa aaagatcttg ggaaaagact
     3121 caatttcagt agaaaaggaa gtaaatcttc ccacattaaa acgcatagta tcaagttttt
     3181 tcaatacctg atagtatgcg tttttgcttt taacgacttt ttgcccagtc tcttattttt
     3241 cgataatttt gtgggcgatc aattgacggt aacaaatttg tttattgttt ttattatcat
     3301 taataatctg gagaatagtt tcaagggaaa cacgaccaag ctctaaacta ttgatatcga
     3361 cataggctgc caagttgagc ttgggattaa ccgagtcaaa gctgagaaca gggacatcca
     3421 gctggtgttt ggcaatatag ttacaaacgc cttcagctag gaggctatcg gttgtgatga
     3481 tagcatcaat ttgtggatcg tgcttgaata atcgcttgct aaatttatag cccttttctt
     3541 ccagaaactc gtcggcaaag tagatgcgat tgttgtcagt ggtaagtttg taatgtttaa
     3601 gcgcctgttc atagcctgtt aaacggtctt tggtcacgaa gagcttttta cttcctccga
     3661 taaaggcaat gcgtttgcag ccttttttga tgaaatattc agtcgcatca aaaccagctt
     3721 gaacattgtc gttgtcgaca agtgggatga aaggagatag agatttacct aagataagga
     3781 aggggaactg ttcttctgcg acgagtttta cgagagggtc ttcttcttgg gcatagagaa
     3841 agattagccc atctacacgc ttgccgtaga ccatttgtga aatagcgttg agacgctcct
     3901 tctcatcttt ccctgttgct atctgaatgg catagtggtt ttcagatgcg acttgagaga
     3961 tgccacgtag aaccgatgga aagaaaggat tctggtagaa ggcgtctgag tcatcaggaa
     4021 gaactaatcc gataacctga gtatagctgc ttaccaagct acgagcgttg aggtttgggt
     4081 ggtagttgag ttccttcata gctttgcgaa cgcgtttttt tgtttcgtcg ctaatggttg
     4141 atttattttg aataacacgg gttacggttg aaggcgaaac accagcagcc ttggccacgt
     4201 ctttaatcgt aacgggcata aaaatctcct atttatggta atttggatca cggaaatgtg
     4261 ttttataaaa gatagtgacc agatacagaa caaaaagaat attttgtaca gtaatcaggg
     4321 ttgtcatatt ttggccaaat aatcccaaaa taagtgtaat cagagtcggc aatcctaaac
     4381 agttcaagat aaagtgatag cactctttaa aggttctaaa tgaaaagagg cgtgatttct
     4441 tagtgatata aaggagaaga ctagctccta gagagacgat aaagaaattc aaaccaaaga
     4501 ggaagctcgc accgagaact aggaagaggc tgatatagat acgattttgt tggtaccagt
     4561 ctttagaaat tgcttgggtc aagctgtctt tgcttttgaa actctcagtc tgaatggctc
     4621 ggtaagagat gcgggtcagt tccttgcttt ccttgctgat gactagttca tttgtatcga
     4681 aatgcagttg caagtcctta ggtaattcct tgatttgact tggaccaatc acaacagaag
     4741 gggcttgact agctgttcca gtataagtta atgtgccatc gacaattgta gcatgttcag
     4801 agagatcctg gacaacctca tttgtcagag gttcatagac attatcgata aaagtttcta
     4861 gcggataggt ctcctgggag ctgttttgga tagcaatggg taccatagac aagctgataa
     4921 ggaagatact ggtaaagaga agttgaaacc agttgagctc gaaacgtttg gacaggggct
     4981 tacgaaaccc ccaaatactt gaaaaatagg aaaatggata tggaagcata ggtatctttc
     5041 taaaaggtgt tttctatatg agtattatta tatagagaaa tgcgctttat ttcaagtcta
     5101 ggagaaaaat tgcttgctac aaggatatta tttacagtga caattgctaa taatcaatga
     5161 aaatcaaagt gcaaaccagg aagctagctg caggttgctc aaagcacaac tttgaggttg
     5221 gagataaagc tgacgtggtt tgaagagatt ttcgaagagt ataaaatgaa aaagggtggc
     5281 ggggataaat tatcccttgt cgccaccact tgtaagtcct gaaacaaagt tcttttgtag
     5341 aaagaagaag agaatacaga ttggaagggc gatgaggata gcacctgctg agaagtaggc
     5401 aatcttcaag tttttcgcat tgttaacgaa ggtttggaga cctacggcaa cagtaaagta
     5461 ttctttctca cgaagcaaga aactagagag gatgtagtcc ccgaaaggtc ccatgaaggc
     5521 ccagagagct tgtacggcaa ccattgggcg aacaagtggt agaacaattt gccagaagcg
     5581 gcggaagtgt cctgcaccgt ctagttttgc agattcgtct aaagacattg gcactgtatc
     5641 gaagtagcct ttcatgagcc aagcattcat cgggatacca ccaccaacgt agaggaagat
     5701 gaggaaccag ttgtggttaa gggcgttcaa cataagcgcc ataacgaaga aggctgtcaa
     5761 agcggccata gttggcacca tttggatgat caagaagaag accaaacttt gtttacgagc
     5821 caagaagttg taacggctgt aagcataacc agcaagtacg atgatacttg tttgaacagc
     5881 catggtaatt aaggcgataa tcaaagtgtt gaggtaccaa gtaccgtaca aggtttcagt
     5941 gaagaggcct ttaaagttat caaaattgag gtcgatatta gtatctagtt taaaggctga
     6001 gacgttacct gctttaaagg ctgacataat ggtaatcaac agtggataga taattacaat
     6061 tgatagacca atcaggtaaa ggtaagtaag gctttgagtc agtctacgtt tgagtttaat
     6121 tgagttattc atcttagacg tcctccatat caaatgcgtg tagtttcttg aatgcgatca
     6181 tagagattga gatgacaatg atagagataa tcaaggtaac agctgccgcc attgagtatt
     6241 gaggagatgt acctgttgtc aaacggtaga tccatgagat caagatatcg gttgaaccag
     6301 ctccacctcc gacactacca ggtcctccac cattgaagag gtacatgata gagaagttgt
     6361 taaagttgaa ggtgtattgg ctaatcaaag taggtgccgc aacagccaaa atcattggga
     6421 aagtgatgtt gcggaatttt tgccaagcgt tggcaccgtc aatataagct gcttcgtaaa
     6481 ggtcgttagg aatagattgc aagataccca aagtcaaaac gtagatgtat gggaatccga
     6541 gccaaccttg catcataatc aaggcaatct tagtccaagt tgggtctgtt ttccaaggaa
     6601 taagagctcc atcaaggaaa ggaaggaatt tagccaagat tggcaatact tgagtgttga
     6661 tagcaccgac actatcgtta aacatgtttg agaatgtcaa gatagtgatg aaggctggga
     6721 cagcccaagg aagaaggaaa ataacaccaa agatacgttt tcctttgata aatggttggt
     6781 tggcaatgat agctgtgaag ataccaatta cgatttgtaa agtagaagct gccaaagccc
     6841 aaatgatagt ccaagaaaga acagaaccaa aggcagaacg gaaggtactc aagctccaaa
     6901 tgtttgtaaa gttggtcaaa ccaacccagt ccaacaactt gtttggtggc aagtgttgga
     6961 agtcgtagtt ggtaaaggcg atcatcaagg ttacgataac tgggaagata atcgcgaagg
     7021 tcatggcaac ataagatgga atgatcaaga ggtaagggaa gccattttca taaatccctt
     7081 tgatcatgtc tttgagtgtg cgtggaactg gaattccatt gttaatgcgt tttgcaatcg
     7141 tatgtgcatc tttgatattt gagaaataaa agagtacata aacgattaca aagattagat
     7201 ggaaggcacc acgaatcagc ataaagaggg aattatcacg acctggtttg ttaccaagag
     7261 tgatgaggtt gctcaattca ggggttgcaa gtgctaggaa gtaaaggaca aatacgatgg
     7321 ttacaccaag gaagataaaa cctttggctt tttgtttatt gtaaatctgt cctaacccag
     7381 gaatgataga cagcagggct gctttactag gttgttgctt ttccatatta actcctttca
     7441 taggatacta cttttttata agaaagctaa attatttgtg tctctattga taaattcaaa
     7501 gggggatttg atttccaccc cccttgaaca aattttttat tcaccaaatt tttgtttgat
     7561 tgtttctttg atcaatgtta cagcatcgtt agcagctgtt ttagcatctt tttgaccact
     7621 tacagcatca aagagcatat ttttcgctgg atcccaaact gcagacattt gagagatgtt
     7681 tggcagtggt tgagtgttct tgaactgttt gataacagct gttgtcaact catcgttttt
     7741 accttcagcg tatgaacgag cctcagtatt agctgggatt tcgttagtct tatcatataa
     7801 tactttttgt tgttcagttg caacaaggaa gtctacaaat ttttgagaag cttcaaggtt
     7861 cttaacggct tgaggaatga cccaagcttt accaccaccg aatgcagcat attcttttcc
     7921 atttggaaga gttgggatag ttgcaactcc gtagtttact ttagcatctt taaaggcttg
     7981 agctttccaa ggtccgtcga tgatagcagc tgttttacct tcttggaatt gagtttggat
     8041 taagtttcca gcaccttctg tatcttgcat acctttaggc catttttcgt accaagattt
     8101 agcgtaattg atacctacga tagaaccgtc gtttgcaaga ccgatgtctt tagcgtcttt
     8161 accgttttgg ccaaagacgt aagcaccgtt accggcaaga agtccatatg tatagtagaa
     8221 gtttgtccag tcagctagga aggcagtagt tttaccatct tcaccagcga atgcgtattt
     8281 gctatcttta gcaaggtttt ccaagtcagc aaatgttttt ggagcatctt tcaccaagtc
     8341 tttgttgtag tacataacaa gtgactcgat aacggcagga gcaccgtaaa ctttaccatt
     8401 agcagctgtt acaagagatt tagttgtgtc gtctgtttta gcaccatcgc tcaatttcac
     8461 ttctgaaagt tgtccgtcag aaccaaggct acctacacgg tcgtatggag ccatcataac
     8521 atcagggaca ttaccagatt ggttgtcaag agaaagttta tcaagacctc ctagagcatc
     8581 accagtttta agagtgactt ttactccagc ttctttttca taagctttag caacctcttc
     8641 aatatagctc ttatatccct cgtctacata tacagtgagt tctttgactt cagatgaacc
     8701 agaatcagca ggcttatcag cagttttgct tccgcaagct accaaaagca agctagcaag
     8761 tgtagcagtt ccaagcaccg cagtgctctt cataaattta gatgacatag tgtattcctc
     8821 ctaaagaata gcaagtttta ttgataagga aacgcaaacg ttttccttta tgagcttagt
     8881 atagcacaaa tagaaaacgg ttgcaagtat tttttgtaaa aattttaaaa aatttttaag
     8941 cttgatttga tagcataatt ttgcatttta atagaagaaa agtatgaaat agataagaat
     9001 acagtatttt taataaaaat atatggaatc tgtctaaaga gggaaatcag gaggggctac
     9061 ctccctgttt ctaatctata tctattttct atagacgttt gtgctttgtt acatataaat
     9121 tcaagctccc taaggaggtt ttatactttg tagactgtct cgtgagagtc aaaccttagg
     9181 attagatagt tttaagttat ctttgctaaa atgagttagt ttccttaaaa ataatcaaaa
     9241 aagtttttta aaaacgcttg caattatgct tgaaaaggag tatacttata agtaacgcaa
     9301 acgtttgcgt ctgcaaaata cgcaacgttc cattatttta acacacgagg tgctattatg
     9361 aaaaaacgtc aaagtggtgt gttgatgcac atctcttctc ttccaggagc ttacggaatc
     9421 ggatcatttg gtcaaagtgc ttacgacttc gttgatttct tggtccgtac aaaacaacgt
     9481 tactggcaaa tccttccatt aggagcaact agttacgggg attctcctta ccaatctttc
     9541 tcagccttcg caggaaacac tcattttatc gatttagata tcttggtgga gcaaggtttg
     9601 ttggaagcaa gtgaccttga aggagttgac tttggtagcg atgcgtctga agttgactat
     9661 gctaaaatct actatgcacg tcgtcctctt ttagaaaaag cggtgaaacg tttctttgaa
     9721 gtcggagatg ttaaagattt tgagaaattt gctcaagaca accaatcatg gcttgagctc
     9781 tttgctgagt atatggctat caaagagtat tttgacaatc ttgcttggac tgaatggcca
     9841 gatgcagatg ctcgtgctcg taaagcttca gcacttgaaa gctatcgtga gcaattggca
     9901 gacaagttgg tttaccaccg tgtgactcaa tacttcttct tccaacaatg gttgaaattg
     9961 aaagcttacg ctaacgacaa ccacatcgaa atcgttgggg acatgccaat ctacgtagcg
    10021 gaagattcaa gtgatatgtg ggcaaatcca catctcttca aaacagatgt caatggtaag
    10081 gctacttgta tcgcaggatg cccaccagat gagttttctg taactggtca gctttggggt
    10141 aacccaatct atgactggga agcaatggac aaagacggct acaaatggtg gattgaacgc
    10201 ttgcgtgaaa gcttcaaaat ctacgatatc gttcgtatcg accacttccg tggcttcgaa
    10261 tcttactggg aaatccctgc tggttccgat acagcagcac ctggtgagtg ggtgaaaggt
    10321 ccaggctaca agctttttgc agccgttaag gaagaacttg gtgagctaaa catcatcgca
    10381 gaagaccttg gcttcatgac agatgaagtg atcgaattgc gtgaacgtac tggcttccca
    10441 ggaatgaaga ttcttcaatt tgccttcaac ccagaagacg aaagcattga tagcccacac
    10501 ttggcacctg ctaactcagt tatgtacaca ggaacacacg ataacaatac ggttcttggt
    10561 tggtaccgta atgagattga tgatgcgact cgtgagtaca tggctcgtta cacgaaccgt
    10621 aaagaatacg aaacagtggt acacgctatg cttcgtacag tattttcatc agttagcttt
    10681 atggcaattg caactatgca agatttacta gaattggatg aggcagctcg tatgaacttc
    10741 ccatctaccc ttggtggaaa ctggtcttgg cgtatgactg aagatcaatt gacaccagct
    10801 gtcgaggaag gtttgcttga cttgacaaca atttatcgcc gaattaatga aaatttggta
    10861 gatttaaaga aataagacaa taatcaggag acaactaaac atgttatcac tacaagaatt
    10921 tgtacaaaat cgttacaata aaaccattgc agaatgtagc aatgaagagc tttaccttgc
    10981 tcttcttaac tacagcaagc ttgcaagcag ccaaaaacca gtcaacactg gtaagaaaaa
    11041 agtttactac atctcagctg agttcttgat tggtaaactc ttgtcaaaca acttgattaa
    11101 ccttggtctt tacgacgatg ttaaaaaaga acttgcagct gcaggtaaag acttgatcga
    11161 agttgaagaa gttgaattgg aaccatctct tggtaatggt ggtttgggac gtttggctgc
    11221 ctgctttatc gactcaattg ctactcttgg tttgaatggt gacggtgttg gtcttaacta
    11281 ccactttggt cttttccaac aagttcttaa aaacaaccaa caagaaacaa ttccaaatgc
    11341 atggttgaca gagcaaaact ggttggttcg ctcaagccgt agctaccaag taccatttgc
    11401 agactttact ttgacatcaa ctctttacga tattgatgtt actggttatg aaacagcgac
    11461 taaaaaccgc ttgcgtttgt ttgacttgga ttcagttgat tcttctatta ttaaagatgg
    11521 tatcaacttt gacaagacag atatcgctcg caacttaact ctcttccttt acccagatga
    11581 tagtgaccgt caaggtgaat tgctccgtat cttccaacaa tacttcatgg tttcaaacgg
    11641 tgcgcaattg atcatcgacg aagcaatcga aaaaggaagc aacttgcatg accttgctga
    11701 ctacgcagtt gtccaaatca acgatactca cccatcaatg gtgattcctg aattgattcg
    11761 tcttttgact gcacgtggta tcgatcttga cgaagcaatc tcaattgttc gtagcatgac
    11821 tgcctacact aaccacacaa tccttgctga agcgcttgaa aaatggcctc ttgaattctt
    11881 gcaagaagtg gttcctcact tggtaccaat catcgaagaa ttggaccgtc gtgtgaaggc
    11941 agagtacaaa gatccagctg ttcaaatcat cgatgagagc ggacgtgttc acatggctca
    12001 catggatatc cactacggat acagtgttaa cggggttgca gcactccata ctgaaatctt
    12061 gaaaaattct gagttgaaag ccttctacga cctttaccca gaaaagttca acaacaaaac
    12121 aaacggtatc actttccgtc gttggcttat gcatgctaac ccaagattgt ctcactactt
    12181 ggatgagatt cttggagatg gttggcacca tgaagcagat gagcttgaaa aactgttgtc
    12241 ttatgaagac aaagcagctg tcaaagaaaa attggaaagc atcaaggctc acaacaaacg
    12301 taaattggct cgtcacttga aagaacacca aggtgtggaa atcaatccaa attctatctt
    12361 tgatatccaa atcaaacgtc ttcacgagta caaacgccaa caaatgaacg ctttgtacgt
    12421 gatccacaaa taccttgaca tcaaagctgg taacatccct gctcgtccaa tcacaatctt
    12481 ctttggtggt aaagcagctc cagcctacac aatcgctcaa gatatcattc acttgatcct
    12541 ttgcatgtca gaagttattg ctaacgatcc agcagtagct ccacacttgc aagtagttat
    12601 ggttgaaaac tacaacgtta ctgcagcaag cttccttatc ccagcatgtg atatctcaga
    12661 acaaatctca cttgcttcta aagaagcttc aggtactggt aacatgaaat tcatgttgaa
    12721 cggagctttg acacttggta ctatggacgg tgctaacgtg gaaatcgctg agttggttgg
    12781 agaagaaaac atctacatct tcggtgaaga ttcagaaact gttatcgacc tttacgcaaa
    12841 agcagcttac aaatcaagcg aattctacgc tcgtgaagct atcaaaccat tggttgactt
    12901 catcgttagt gatgcagttc ttgcagctgg aaacaaagag cgcttggaac gtctttacaa
    12961 tgaattgatc aacaaagact ggttcatgac tcttcttgac ttggaagact acatcaaagt
    13021 caaagagcaa atgcttgctg actacgaaga ccgtgacgca tggttggata aagtcatcgt
    13081 taacatttct aaagcaggat tcttctcatc tgaccgtaca atcgctcagt ataacgaaga
    13141 catctggcac ttgaactaat actcttcgaa aatctcttca aaccacgtca gctttatctg
    13201 caacctcaaa gcggtgcttt gagcaacctg cggttagctt cctagtttac tctttgattt
    13261 tcattgagta taagatacaa atttatacta atacatcttg taaaaaagcg agtttcgatt
    13321 gaaattcgct tttttaatga tgtagatttg ggtcaatctt gtctaaaaat agggaaatcc
    13381 tagatacagt gaaggcttta aatgctggtt tttactgtcc tcagccttat attttttcgt
    13441 agttgattac ctcatacctc ttgtattcgc ttacataaag tattataata taattgtagg
    13501 aaagaaggtg tttttatgat atacacactt aaattggtgt tgtttattac ctttcttgta
    13561 ataagcttgt tacctgataa gatttttgga aaaaataaaa aaatttggaa aatagttttt
    13621 gcaatattga cggcagtggc agcattgtca tttatgtact aagttatttt aagaatgtag
    13681 ggaaataaac cctacattct ttttagtttt ttctgttttc taaattctat ttatccaagc
    13741 gcttcaacat ttcttgcttc ttcgcttcaa gttctgcacg cttttcttcg atttcggcat
    13801 gttttttctc gagttcagaa caacttgcac cattgctaaa ttcttttcgc catcaggaga
    13861 tagggtgagt cgacatgtct attactcacc caaagcagtc ctacaaagca ggaattttct
    13921 gttacttttt tggaaatagt aacgtttata cagctttgac acttcgtatc aaagcgccaa
    13981 acacactccg agggatttac agaaagcaga aaaggaatga tctggtataa gatcattcct
    14041 tttctctctt tttctttaag taattatata caatgtacga cgaagtcgtc attgcaatgc
    14101 tgatccacca cctaaaggaa actttaaaca acattgataa gataaagaat ataaacaacg
    14161 aaaatacgtt atacccaatt aattttattg tatatctcat gattaaaagt taatccttcc
    14221 gttgttagga atggcatcat ttttatccca taattgtgct aaataagtcc ccggtgataa
    14281 taaatttata gcaaattcta aagcaacatc atttacaaac caactaccta gatatctaga
    14341 aattgctgaa cgaatagcac tttttgctgc atgttttcct tttactttaa ttagatttgc
    14401 aaggcctgca gtagttcctc ctaatgctaa agctattgca gtatctaata gagcacccat
    14461 ttgattaact gtaatacctt gccaagctgc tctaaatgga gagtatgtag gtgggattgt
    14521 ataatcgcct tgtaattgtc ggttaattac ttctttgatc cattgttgtg agacgtctgg
    14581 atgaaaagat tggatttcgt ttgcaagtgt attgatttgt tcttctgtta gagaagtgac
    14641 aggttgaagt tccatatttg tttcaatttg tgatacttgt tcagaagcgt atacagctga
    14701 aacacttgga atcgctgata caattaacac aattgacgtc aaaaaaaccg aaataaattt
    14761 cattaatttg ttcatgagct tttctccttt ttatttgtat ctgcttacat tttatcatat
    14821 gctgttatta tagtcaaaaa aatatgctat tatgttaaaa aaatattttt caaaatataa
    14881 atggacggat ttattttgga ttttatttgt tattttgacc tgcctctata ttggtaacca
    14941 tgatttgttt actctcaatc atcaagaatt ctcttttcgt ggtagcgttt ggggtctggt
    15001 actggcctta tatcacttac tattcattga taagtttgtt atatcgaatc gaaaataaag
    15061 attagagcta tgcttgactg tgtactttta ggattgattt tggaggaaga ttttgtctct
    15121 attatttatt attttaaatt tatttatttt gtataagatc tattctttga ggcgggaaaa
    15181 atcgaaatac ttgatttata cggcctatat catatttggg gtaaatgtag tatatggtat
    15241 tcaatggtta ttaagagaac tgatttcaac tatttcccct taatgtgtat ggaaactata
    15301 acaaaacgca taagattaag gtttgtgtac ctgttcttat gcgtttttat aatattgaag
    15361 attttcggta agttttatac ctttgggaag tctagtttag aatgctttcc caaccaaaat
    15421 ctctgcttcg atggtaatct ctgtcagttg gctccagtca atcttgctct ggtcaacgtg
    15481 aaagaggaga ggagtcatac tgagtagggc ttggagttgt tctgctgtga tagtcttagt
    15541 cagagaggca gtttgactag ataggatggt aaagtgttcc tggaaatgct ccttgatatc
    15601 ttggttagaa tactccttgt ttgtcagctg gtcctgtact ctttgacgga tttctttgag
    15661 gtgattttca gttgggataa cctttatcaa gataccgtct ttggataaaa cgcgacgaaa
    15721 ttctccatag ttggcaggtg agaagatatc aagcagaata tccatgttag cgtcttttat
    15781 aggaagtcgt gccaagtcgc caacgaacca attgactgcc cagttgggtt cacttttagc
    15841 agcgatttgg actgaatctt tggagatgtc aaaggcatag aaagtttttt cagagtgact
    15901 ttcttgtagt ttgcgagaat agaatccttc accacaaccg atatccaaaa ttgttgtggt
    15961 agtttttgag cttgcaagca agtcagatac agcatctaag atagcttggt aaaagccggc
    16021 ttctaggatt tgttgacggt tttgaaaatt ttccttgtca tagttagcag attgcttgat
    16081 ttgaggtgct agattgacat agccaaattt cgccaagtca aaagaatgac ggttgcagca
    16141 cttgaaatta gtctctaaca gagtcagatt ttcttgacag ataggacagg caaaggcagt
    16201 cgcagaagca aaacgctgaa gtttgggctt gagatttgta ttcatagttt aagtgtatca
    16261 aaagaggcaa atgaaagcaa ctttaggaat aagtagattc agtagaaata aaactaattc
    16321 tccaatttat agaatgaaat tgcttttata tctaaattgc tatataataa tgataaggag
    16381 gaaataagta tgttaaaaga agtattaacc gttgcaaaag ttgcgaaaaa atcttcatta
    16441 tttttgggtg gtgtcgcatt tggtaccctt ggtttgaaaa tcttagcaag taaggaagct
    16501 aaaaaaggtt attctaaagc tttggctaag gcttacaagt tgaaagacga gctagatgca
    16561 tctgtttctg ttgtgaagca acatggagac gatgtcttgc aagatgccaa atatttgtac
    16621 gagcaagaga aaaaagaaga gcaattagat agccttatag gtgaataata tgtcttttaa
    16681 agtgctacat agaggatacc aacatatccg actatcatct tctttttcac ttaccttgga
    16741 tattcaagac tatcttcgtt ccttggcgag agatgaaaag gggattgagt ctatccagtt
    16801 ttacatggat caacagcact ttactctacg cataaaagaa ggcttttctg tattagataa
    16861 tgcagaagcc tttttaaaaa gaattgataa agggaaagtt tctgagttga tgactcttcc
    16921 cattcgtaga gaagagagtg cttattctat tgtttcaggt gcagcggtta agcgtgtact
    16981 ttttcgtagt tttgtgccgt atcctattcg ctatatatgg acttgttatc aggctttggg
    17041 ttatattaga gaagcctatc aaacactagc gcgtaaggaa ctaacgatgg aagtcttgga
    17101 ctgttcggct attttattgt ccttgtttat gaaccaatcc aagacagcta gcaatatcat
    17161 gtttatgctt gatttgggga atcatttaga tcagtggtcc ttgaaaaaaa ctgcaacaga
    17221 tttagaacag agtcttcttg caaaagagag cgatgtattc ctagtacagg gcgatacggt
    17281 tgttagtatc aagagttccg atgttcaaat aggagatgtc ttgatcttat ctcaaggaaa
    17341 tgaaattctg tttgatggac aagtagtttc aggtttaggt atggtcaacg aaagttcctt
    17401 gacaggagag agttttccag ttgaaaaaag agagtctgat ttggtttgtg caaatacagt
    17461 attagaaact ggagagttac gcattcgtgt aacagataat cagatgaaca gccgtatttt
    17521 acagctgatt gagttgatga agaaatctga agaaaacaag aaaacgaaac aacgctattt
    17581 catcaagatg gcggataaag tcgtcaaata taatttcttg ggggctgggc tgacttacct
    17641 attgacaggt tctttttcta aggctatttc tttcctattg gtcgatttct cctgcgcttt
    17701 gaaaatctct actcctgtag cttatttgac agttatcaag gaagggttga accgtgaaat
    17761 ggtgattaag gatggagatg ttctggagaa atatctggaa gttgatactt tcttgtttga
    17821 taagacagga acaatcacaa ctagttatcc tatagttgaa aaggtgttac cttttggaga
    17881 ctatagtgag gaagatattc tcagaatcag tgcctgtctt gaggaacaca tttatcatcc
    17941 tattgctaat gccatcgtca agcaagctga gatagaggga attgaacatg aggaaatgca
    18001 tgggaaactc caatatatcg caagcaaggg gatcaaatct catatagatg gccaaccagt
    18061 tcttattggg aattatgtct tgatgcagga tgagcagatt catatcagtt cagaacaaaa
    18121 tgctttaatt gaagagtaca agagtcacta caatctctta ttcttggctt atcaaaatga
    18181 attgattgga atgttctgca ttcatactcc tttgagaaaa gaagcaaaaa cagccttgga
    18241 taaacttaag gcacagggga aaaaattgat tctggcaaca ggagacacct tgattaggac
    18301 agaggaatta gtcaaagatt tgccctttga tcaggtctat acagacttga aacctgatgg
    18361 gaaatttgag ttagtagaga aactgcagaa agcaggtcac actattttga tggttggaga
    18421 tggattgaat gactcagcgg ctttaactct atcagatatc ggtgtggtga tgaatgagag
    18481 tgcagatatt tctaagcaga tgagtgatat cttattgtta gataatcgtt tggatttctt
    18541 ccaagagttg gattcgttat catcatcttt gcaaacactc atcaagaaaa atattcagga
    18601 taccgttgtc gtaaatagta gtttgattgg ctttggctta tttaattggt tcagtccttc
    18661 aaatctctct atcttacata atctaacaac cttgcgcatt gtcctgcgta gcctgtctat
    18721 taaaaataga tagatgagag ttattaacag caaaaaggag ttacctttct cgaggttaac
    18781 tcctttgttt atagataaat gttgaatagt ttagtaagtc aatacaaagt attttttctt
    18841 cccacgacgg ataacagtca gttcattttc taacttatca gcgtcactca agacatagtc
    18901 aaggtcttgg atgcggtcgc cgtttacgta gatggctccg ttttggacgt cttcacgggc
    18961 ttggcgtttt gagttaacta taccagatga gacgagcagt tccacgatat tgttgttttc
    19021 gtctgcctgt acttggtagt ttggcacacc acgaagtcct tgtttgagct ctttgacaga
    19081 aaggtttttg atgtttcctg caaagagttg ctcagtgatg ttaagtgctt ctttgtaggc
    19141 ttcttctccg tgaacaagtg taacaacttc acgagccaag actttttgag ccaagcgttc
    19201 gtgtggcgct gcttcaaatt gtttacgaat atcttcaatc tcatcaagtg acaagaaagt
    19261 aaagattttc aagaagcgaa cagcgtcagc gtccatcacg ttcatccaga attggtacat
    19321 ttcgtatgga gaagtctttt cgggattgag ccagacggca tttccttctg atttaccaaa
    19381 ttttttacca gttgcatctg tgattagtgg aacagtgata acgtgaccag tcttgtccgc
    19441 cttacgacga agcaattcgg taccagctgt catatttccc cactggtcag aaccaccgat
    19501 ttgaagagtg acattatggt cttggttaag gacgaagaag tcgtaccctt gcatgatttg
    19561 gtaagcgaac tcagtgtaag aaattcctgt ttcgatccgt tttttaacag attccttact
    19621 catcatgtag ttgaccgtga agtactttcc aatgtcacgg aggaagtcaa tgaagctgat
    19681 gctgccaaac cagtcgtagt tgttgaccat gacagccttg ttttcgccat tttcaaagtc
    19741 aagaaaacga gaaagttgtc cttggataga cttgacccag ccatctactg tgtcttttgt
    19801 ttggagacta cgttcagcat ctttgaagga cggatctccg atgagacctg tagcaccgcc
    19861 aacgagcgca taaggtttgt gacctgctag ttgcaagcga cgacttgtca agattgcgac
    19921 aaggtggcct aggtgaaggc tgtcagcagt tggatcgtag ccagtataat aagaaacttg
    19981 accttcttct agggctttac gcaaagcttc ttcatcagtc gtttgaaata tcaaaccacg
    20041 ctcttttagc tcatcaaaaa tgtgcatgtg tcttttctcc tttttaaaat tattgtttct
    20101 acctattgta tcacaaactt gggcaatagc ctagcagaat agggaagaaa gtggctattt
    20161 tattgaaaat ttcccttttg tggtataata gataaagtga ggaaatccat gcaaaatcaa
    20221 ttaaatgaat taaaacgaaa aatgctggaa tttttccagc aaaaacaaaa aaataaaaaa
    20281 tcagctagac ctggcaagaa aggttcaagt accaaaaaat ctaaaacctt agataagtca
    20341 gccattttcc cagctatttt actgagtata aaagccttat ttaacttact ctttgtactc
    20401 ggttttctag gaggaatgtt gggagttggg attgctttgg ggtacggagt ggccttattt
    20461 gacaaggttc gggtgcctca gacagaagaa ttggtgaatc aggtcaagga catctcttct
    20521 atttcagaga ttacctattc ggacgggacg gtgattgctt ccatagagag tgatttgttg
    20581 cgcacttcta tctcatctga gcaaatttcg gaaaatctga agaaggctat cattgcgaca
    20641 gaagatgaac actttaaaga acataagggt gtagtaccca aggcggtgat tcgtgcgacc
    20701 ttggggaaat ttgtaggttt gggttcctct agtgggggtt caaccttgac ccagcaacta
    20761 attaaacagc aggttgttgg ggatgcgccg accttggctc gtaaggcggc agagattgtg
    20821 gatgctcttg ccttggaacg cgccatgaat aaagatgaga ttttaacgac ctatctcaat
    20881 gtggctccct ttggccgaaa taataaggga cagaatattg caggggctcg gcaagcagct
    20941 gagggaattt tcggtgtaga tgccagtcag ttgactgttc ctcaagcagc atttttagca
    21001 ggacttccac agagtcccat tacttactct ccttatgaaa atactgggga gttgaagagt
    21061 gatgaagacc tagaaattgg cttaagacgg gctaaggcag ttctttacag tatgtatcgt
    21121 acaggtgcat taagcaaaga cgagtattct cagtacaagg attatgacct taaacaggac
    21181 tttttaccat cgggcacggt tacaggaatt tcacgagact atttatactt tacaactttg
    21241 gcagaagctc aagaacgtat gtatgactat ctagctcaga gagacaatgt ctccgctaag
    21301 gagttgaaaa atgaggcaac tcagaagttt tatcgagatt tggcagccaa ggaaattgaa
    21361 aatggtggtt ataagattac tactaccata gatcagaaaa ttcattctgc catgcaaagt
    21421 gcggttgctg attatggcta tcttttagac gatggaacag gtcgtgtaga agtagggaat
    21481 gtcttgatgg ataaccaaac aggtgctatt ctaggctttg taggtggtcg taattatcaa
    21541 gaaaatcaaa ataatcatgc ctttgataca aaacgttcgc cagcttctac taccaagccc
    21601 ttgctggcct acggtattgc tattgaccag ggcttgatgg gaagtgaaac gattctatct
    21661 aactatccaa caaactttgc taatggcaat ccgattatgt atgctaatag caagggaaca
    21721 ggaatgatga ccttgggaga agctctgaac tattcatgga atatccctgc ttactggacc
    21781 tatcgtatgc tccgtgaaaa gggtgttgat gtcaagggtt atatggaaaa gatgggttac
    21841 gagattcctg agtacggtat tgagagcttg ccaatgggtg gtggtattga agtcacagtt
    21901 gcccagcata ccaatggcta tcagacctta gctaataatg gagtttatca tcagaagcat
    21961 gtgatttcaa agattgaagc agcagatggt agagtggtgt atgagtatca ggataaaccg
    22021 gttcaagtct attcaaaagc tactgcgacg attatgcagg gattgctacg agaagttcta
    22081 tcctctcgtg tgacaacaac cttcaagtct aacctgactt ctttaaatcc tactctggct
    22141 aatgcagatt ggattgggaa gactggtaca accaaccaag acgaaaatat gtggctcatg
    22201 ctttcgacac ctagattaac cctaggtggc tggattgggc atgatgataa tcattcattg
    22261 tcacgtagag caggttattc taataactct aattacatgg ctcatctggt aaatgcgatt
    22321 cagcaagctt ccccaagcat ttgggggaac gagcgctttg ctttagatcc tagtgtagtg
    22381 aaatcggaag tcttgaaatc aacaggtcaa aaaccaggga aggtttctgt tgaaggaaaa
    22441 gaggtagagg tcacaggttc gactgttacc agctattggg ctaataagtc aggagcgcca
    22501 gcgacaagtt atcgctttgc tattggcgga agtgatgcgg attatcagaa tgcttggtct
    22561 agtattgtgg ggagtctacc aactccatcc agctccagca gttcaagtag tagttctagc
    22621 gatagcagta actcaagtac tacacgacct tcttcttcaa gggcgagacg ataattctct
    22681 aaatgaagtg gccaatcaat ggattgccac ttttttcctt tcgtgttaca ataagggtat
    22741 gaatcagtat cagaaaaaga ttgttaacgg aaaaatttat tcgctcctat ccggcttaat
    22801 atggggaatc tgtggaattt taggagagta cttctttact cattatcagg tgtcttcggg
    22861 ctggattacc tctatgcgtt tgacactggc agggagtctt gtactcattt ggtctgcaat
    22921 acaattaaaa tcgcaagtgc tagatatttg gcgagacaag aaaaattacc tgcccttttt
    22981 agcctatgct attttgggga ttttttcagt tcagtatttt ttctatctct gtttagaata
    23041 ctcaaatgct acgacagcaa ctattttaca gtttattagc cctgtcttta tcctctttta
    23101 caatcgcttg gtttatcaaa aacgagcgtc aaaaagcgct gttttctatg ttttggttgc
    23161 catgctgggt gtttgcttga tggcgacaaa gggagatctc tctcagttat ccatgacgcc
    23221 gctagctctt ataacaggtt tgctgagtgc catgggtgtt atgtttaatg ttatcttgcc
    23281 ccaacctttt gctaagcgtt atggttttgt tcctacggtt gggtggggga tgattttggc
    23341 aggtttgttt agcaatgtcc tctcgccggt ttatcagctt tcctttactc ttgatatttg
    23401 gagtatcttg atttgcctca ttatcgcttt ctttggaacg gcttttgctt ttttcatttc
    23461 catgaaggct gtgtccttgg tttctccttt ggtggtttcc gttatcagtg ccagtgaacc
    23521 tctctcttct gctctcttga gtgttttgtt cttaggatta gtagtggatt ggtccctcct
    23581 tctagctata gccttgatta ttttacccat gatatttttg tctatagaag aagcgaaaga
    23641 aagtagataa aaagtctttt cttaattcta aaagtgtgct atactagaat agtcaataaa
    23701 acacggggag atagttgtga agagtatttt taggttgtat cggtaggggc ttgcttttac
    23761 cgacagcacg ttttatctca catccctaaa aaatcatacc taaaaacaga caaaaaggtt
    23821 tcaaatttga ggcctttttt ggtagaatag gtatcattat aacgaaccag gaggcaccta
    23881 tgactgctac aaaaatgaac gctcaagaaa ttatccaatt tatcgccaat gctgaaaaga
    23941 aaaccagtgt caaagtaacc tttgaggggc aactcgcaac tgctgtgcct agctctgttg
    24001 tcaaactagg aaatgtccta ttcggagact ggaaggacgt ggctccgctt cttgaaggtt
    24061 tggtagaaaa tcaagattat gttgtcgagc aagatgctcg taattctgca gttcctttgc
    24121 tagataagcg tgctatcaac gctcgtatcg agccaggtgc tattatccgt gaccaggtgg
    24181 aaattggtga caatgctgtt atcatgatgg gagctgttat caatatcggt gctgaaatcg
    24241 gtgctggaac catgattgac atgggtgcca tccttggtgg ccgtgctatc gttggaaaaa
    24301 atagccacgt tggtgcaggt gcagttttgg caggtgtgat tgagccagct agtgctgaac
    24361 cagtctgtgt cggagacaat gttcttatcg gggctaatgc agtggttatc gaaggagtcc
    24421 aaatcggtag tggttcagtt gtcgctgcag gagctatcgt tacccaagat gtcccagaaa
    24481 acgtggtagt agcaggtgtt ccagctcgta ttatcaaaga aattgatgcc caaactcaac
    24541 aaaaaacagc gctagaggat gcgcttcgta ccttgtaatt gtaaaagtaa aaaagaggcg
    24601 gaaccctttt tccagcctct ttctgctata tcggaggaca gatagatgtt agatttgatt
    24661 cagactagac gagctttaca ccagattcca gagattggct tggaggagtt caagactcag
    24721 gcttatttgc tggatgtgat tgagaaattg actacgggca aggattttgt tcaaattcgt
    24781 acttggcgga cagggatttt ggtctacctg cagggaagtc agccggagcg aaccattggt
    24841 tggcggacag atattgatgg cctgcctatc gtcgaacaaa caggtctacc ttttacttct
    24901 caacaccaag gtcgcatgca tgcctgtggt catgattttc atatgaccat tgccttaggc
    24961 tgtcttgagc gcgcccttga ggagcaaccc aagaataatc tgctctttct atttcagcct
    25021 gctgaagaaa atgaagctgg tggtatgctc atgtatgagg acggtgcttt tggagattgg
    25081 ttgccaaacc aattctatgg tctccatgtt cgtccagatt tgaaagtcgg ccagattgcg
    25141 actaatactc atacactctt tgcaggaact tgcgaggtga agatccgttt caaaggtaaa
    25201 ggtggccacg cagcttttcc tcatgaagcc aatgacgcct tggtggcggc tagttacttt
    25261 gtgacccaag tgcagtcagt tgttagccgc aatgtcaacc caatcgaggg agcagtggtg
    25321 accttcggcg tttttcaagc tggaaccacc aacaatgtca tcacagacac agctttttta
    25381 catggaacta ttcgtgcctt gacacaggac atgagtctct tggtgcaaaa gagggtaaaa
    25441 acagttgcag aaggggttgc agctgccttt gatatggaag tcgaagtgga actcaaacaa
    25501 ggtggatacc tacctgttga gaacaatcca gccttggcgc gtgaactgat ggacttcttt
    25561 gacgagaaag acggaatcga gttgattgat atcgaacctg ctatgacagg cgaggacttt
    25621 ggttatctcc tttcaaaggt tgatggcgtg atgttttggc taggtatcga tagtccctac
    25681 gctcttcatc accctcaaat gagtcctaag gaagaagtct tagctattgg ggtggctgcg
    25741 gtgtctagtt tcctgaaaaa gaaggcagca gaatagaggg cttgtctatg aaatcggaat
    25801 tacgcaagca agtcttgcat gaaatgaagg ctatatctca agagcaaaaa caggctatag
    25861 accaagcttt aaccgagcga attttacaac acccctttta tcaagaagcc aaggtcatcg
    25921 caacctacct ctctttttct catgagtttc aaacgcggga actgattgag caggcgctga
    25981 aggacggcaa gaaggttttg acacctaaga cctatcccaa ggggcgcatg gattttgtgg
    26041 tctatgatcc gcaacagttg gtaaaaactt cctttgcctt actggagcca cagggagatt
    26101 tggaagtagt ggatgcatct cagattgatt tgattcatgt tcctggcctg gcttttacga
    26161 cggatggata tcggattgga tatggtggag gttattatga ccgctatctg gaacattttt
    26221 ctggtcgtgc tttgagtacg atatatcctt gtcaaattca ggactttata cctgaaaacc
    26281 atgatattcc tgttcaggag gtattaattg atgaaggaaa tctttgatag acgttaccct
    26341 gtgacgagtt tcttcctctt agtgacggcc ttggtatttt tactaatgtt ggtcactgca
    26401 ggcggaaact ttgacagggc agatacatta tttcgatttg gagccatgta tgggccagct
    26461 attcgcctct ttcccgagca ggtttggcgt ctcttgtctg ccatttttgt tcatattggg
    26521 tgggaacatt tcattgttaa tatgctttca ctttattatc ttggaaggca ggtagaggag
    26581 attttcggtt ctaagcagtt tttctttctc tatcttttat caggaatgat gggcaatctc
    26641 tttgtttttg tatttagtcc taaatcctta gcagcaggcg cctctacctc tctttatggg
    26701 ctatttgccg cgattattgt tcttcgctat gcaactcgca atccttatat tcaacagcta
    26761 gggcaatcct atctgacact ttttgtagtt aacattattg gaagtgttct gattccagga
    26821 atcagcctag caggccatat cggtggtgca gttggtggcg catttctagc agttatcttt
    26881 ccagttagag gagaaaaacg gatgtataat accagccaga gattaggagc ggtagtcttg
    26941 ttcgtaggac tcgccatttt gcttttctac aagggaatgg gaatgtgacg aagttgaaag
    27001 aaaatttgtg taatgaaaaa agataaggca ttttttgaac cttatctttt tattttattt
    27061 tattccttct cagtcaattc ttttccaagt tggatgaggt aattcttcaa atcatctttg
    27121 acttgtgggt gtttgagggc gtagtcgatg gatgttttca tgaagccaaa cttgtctccg
    27181 acatcgtaac gagccccatt gaactcacga gcaaatacac gttgtgtttt attgagggtg
    27241 tcgattgcat ctgtcagctg aatttcattt cctgcacctg gagcttggtt ttcgaggatt
    27301 tggaaaattt caggcgtgag gaggtagcgt ccgataatag caaggtcgct aggagcgtcc
    27361 tctggagctg gtttttcaac aaaggtttca acactgtaaa gaccatcttt tccttcgcct
    27421 tgcggagcaa taaccccgta agcagatact tcgtcatgag ggactggcat gacagcgata
    27481 gtagacgcgt gggtacgctc gtagtcatcc atgagttgtt tggtaagtgg aacagccttt
    27541 tcgtctgtga tatccatcaa gtcatcacca agcataacga caaaaggttc atttccgacg
    27601 aaagccttag cttgcaaaac agcatctccg agaccgcgtg gatgagtttg gcggataaaa
    27661 tgcagacgca tgtcagttgt tttatcaact agcttcaaaa gatctgtttt ccctttttct
    27721 ttgaggttat attccaattc gaagtttgaa tcaaagtggt cctcaataga acgttttgat
    27781 ttaccagtga caactagaat atcttcaata cctgatttga gagcctcttc cacgataaac
    27841 tggatagttg gtttgtctac gattggcaac atttctttgg caagggcctt ggttgctggt
    27901 aaaaatcgag ttcctagtcc agcagcaggg atgactgcct ttctaacttt tgatgtcata
    27961 aaaatccttt ctatagaggg ttaagaccac tcattttctg ctttaaattc attgttcatg
    28021 atgtcataaa tggcatcttt gatattggtt ccgtgataaa taacttggta aatagcctgt
    28081 gtaatgggca tatagactcc aagttcttgg gctagttcat aggctgctcg agtcgttgaa
    28141 attccttcga ttaccatgcc catattagct tctatatcag ctagggattc tcctcgtccg
    28201 agagcatctc cagctctcca gttacgagag tggatggaag ttcccgttac gatcaaatct
    28261 cccacaccag ataagccgct ataggtcaat ggactggccc cgagtgctac ccctaggcgg
    28321 gtgatttctg ctaaacctcg agcgatgatg gctgccttag cattatcacc aaatccaaga
    28381 ccatgtaaag ctccagcacc gacagcaata atatttttaa gagcaccagc agtttcaacc
    28441 ccgataacat ccgtattggt ataaagtcgg aagtagtgat tactaaatag ctcctgaacg
    28501 tattgagctg tttgtaaatc tttagaagca gcagtgatta aagttaggtc acgcacaatg
    28561 gtttcttccg catgactagg ccctgaaaca acgacaatat cactgcggag ctgttcagga
    28621 atttcttctt caaggatagt tgataatcgt ttatggctat caggttctaa tccctttgaa
    28681 gcgtgcatga tgatagcctt atggtccaag gtttgtgcaa cttgttgggc aacaagtcgt
    28741 gtcacttttg ttgggacaac aaacaaaatc gcatccacat ctttcaatgt ttctgctaag
    28801 tcggtgtagg caatgatatt ttcatctaga acgacatctt taaagtagtg cttgttagta
    28861 tgataggtat taatttcatt gatttgctcg ggaagatttc cccaaatacg tacctcgtgt
    28921 ccattgtcat ttaagacttg tgaaagggca gttccccaag aaccaggccc caagacggcg
    28981 acggtttgtt tttccatgat ttcctccttg atagagttct ttccattatt ctatcatatt
    29041 ctagaggatt ttgcacttgc attactgggg tatggtggaa aaatacacaa agaccgagat
    29101 gaagggataa actcttagtc tttagttaaa gtgagaagaa atctgataga tagtttgatt
    29161 cagtatatgc tataaataga ggtaaacaac tcaggaggtt cttgtaggtt gcgagagttt
    29221 ggcgaaaaaa ttaaaagatt acgtttggct aaaaaaatca gtcgttcaga attttgtggt
    29281 gatgagtctg aattaagtat ccgtcaatta attagaattg aaaatggaga atccagacca
    29341 acactaacaa agttaaaata tatcgctgaa cgtttggggg gggtgaagat tacaagttga
    29401 tgccaagtta tatagagttg gataaggaat acctagaatt gaagtatttc ttgatgagga
    29461 ctcctacata cgaagatgaa actatcgccc aaaagaaaga gagtgttttt gctaagattt
    29521 ttgaagagta ttatgatagg ctacctgagg aagaaagatt tatcatccca aattattcat
    29581 atctagcact agcgaactac acagttcaaa aattaccaga aaagctagtt gaaatactgt
    29641 ccttctggtg attttgctta atttctgatg ttcgagacat gattgaggag atttgtcttg
    29701 attttttcct aaaaaagggc agactcctcc cttggtttga tgctcgattt ttaaaataag
    29761 gtgacttatt ctgttaggca tgttttcccc tgccctctag ctctatatgg aacagggagt
    29821 atcagattta tctggcagat tgtatcaaat aaggcttgaa actgttttga ataggcgtat
    29881 agacttgaga atttgagaat atgtcgtcta gcagtagaga catattttcc ggcaatatga
    29941 aggaagagac gtcgaaaacg cttgatagtc aaggacttta cttcatcacc agctagctgc
    30001 tttaaaaaga ggtagagatt ataagccaga cagcccatca tcatacgtac ttcattctta
    30061 atcatggtcg aactatctgt cttatcccca aagaatcctg ctttcctttc tttgataaag
    30121 ttttcagcat tccctcgttc acgatagaat tgaaacagct cctcactatc tacactgctt
    30181 aaattgttca aatgaacttt tttacaacca taaaaaagag cacttaatcg tactcttctc
    30241 ttttaaggat tagttgaatt aattcaaatc cactagttct cctgtttcta ggtagactag
    30301 gcgttcacaa atgttagcaa tgtaatcagc gaagcgttcc agatgtccta tgatataaag
    30361 gtattgagtt ccattgggaa ttgaggtttc ttggtctttc ataagtccaa tgatttcctt
    30421 tgataaggca taataatatt ggtcaatctg ttcatctttt tgagcaatac taatagcttt
    30481 tgaggcttgg tgcaaaggaa aggcaaccaa taaatcggct agcatgctga gggataattt
    30541 acccatttgg tgtaactgtt cttcgtcagg ggctagttga ttttctttta gttgcaaaac
    30601 agctttggca atgcctgcca tatggtctcc catacgttca aggtctgaac aagaagacat
    30661 gatgctaatc acaaatcgaa ggtcagacac ttgtggctgt tgcaaggcca acaaacgggc
    30721 acaggtcaat tcgatagcgc tttgaccttg gttgatagca tgatccttat tgataattag
    30781 ctctgccatc tccttgtctt tggaggctaa ggccagtaag gcttttgaag ctgtttcaag
    30841 gacaagttgc cctagtccta aaaaggattg ttctaattca tgcaattcta agtcaaattg
    30901 atttctcata ggtttttcct tccttatccg aaccgtcctg aaatatagtc ttctgtgcgc
    30961 tgatcttttg gattggtaaa cacgtcaacg gtatctccaa attcgcaaat ttctcctgtt
    31021 aagaaaaaag cagttttatc tgaaatacgt gaagcttgtt gcatgttatg ggtaacaatg
    31081 ataatcgtat aatccttttt tagttgctga atgaggtctt caatttttaa agtggagata
    31141 gggtctaagg ctgaagtcgg ctcatccatt aacagaacat caggttctac tgctaaagct
    31201 cgcgcaatgc aaaggcgttg ctgctgaccg ccagataagg acatggcact ctttttaaga
    31261 tcatctttga cttcttccca aatggctgcc ccttttaaag atttctccac taaggcatct
    31321 aattgttttt tgtctcgaat accatgtgtc cttggaccat aagccacgtt atcatagata
    31381 gacatggcaa agggattagg ctgttgaaaa accatcccta cacgcttgcg tagctgatta
    31441 aggttgaatt tgctactata aatatcttgc tcatctaaga ggacttggcc ttcaatatgg
    31501 caagaaggaa ccaaatcgtt catccggtta agggttttta gaaaagttga tttgccacaa
    31561 ccagatgggc ctatcaaggc agtaatctgt ctttctggta attgaatcga aatatttttt
    31621 aaggcttgaa aatccccgta aaataagtct aggtgtctga ctgaaaatgt tcccatacta
    31681 ggaagctcct ttcacaagtt ttcgagataa taagcttgat agagtattta tcattaaaac
    31741 agtaataatc aaaatcacac cggtagcata ggcttcattg acatgtagcc cctcacttga
    31801 cagcatatac atatgcaggg ctagagaacg acctgaagac atgagactac ttggcgtatt
    31861 ggtagaggta cctaatgtat acatgagggc agctgtctca ccaacgatac ggccaatagc
    31921 tagtatcact ccagctaaaa tacctggcat ggcaactggt agaacaattc taaaaacagt
    31981 ccgtaactta cctgccccaa gtccataact tgcttgacgc atgctatcac taacagataa
    32041 aagggcttct tctgttgagc gaataatgac tggcaacacc atgatgactg aggttaagat
    32101 tcctgataac agagagtatt gaaaacctaa gaagactaca aagaagagca tgccaaacag
    32161 accaaaaaca atggaaggaa tcccagacaa ggtatctgag gccaatcgca tgattttaac
    32221 acaaagggaa tctttttttg tatattccac aagataaaaa ccagcaaaaa tccctatggg
    32281 caaggctaaa agaagagcac caaagaccag aataacggtg gaaataatcg ctggcataag
    32341 ggaaatgttc tcagaagtat aagtccaaga aaagagggat agacttagat gaggtaagcc
    32401 tttgatgagg ataaaaccaa tgattaaaaa gagagagcca aaggttaaag ctgaaaaaca
    32461 ataaacgaga agttttagca ggtatttact cataagatga ttttcctttc aagtaggcaa
    32521 agtaggcatt aatcaagaga ataaggaaaa agagaactgc tgaggttgca ataagggctt
    32581 ccctatgctg acctgatgcg taagccattt ccagaacaat attggttgtt aaggttctgg
    32641 ttcctgaaaa gagtccactt ggaataatcg gctggttgcc tgccaccaaa ataactgcca
    32701 tggtttcacc tactgcgcga ccgattccta aaataactgc tgataaaata ccagatctcg
    32761 cagctggcaa gatgacacta aaaatactcc gttcatgact agctcctaga gccaagctac
    32821 cagaataata cgttttggga actgttcgga tagcagattc tgacaaactg ataatggttg
    32881 gcaaaatcat tattcctaat agtaacgaag cggttaggac actcatgcca tttcctaaaa
    32941 aggttctaat ccaaggcacc aataattgta ggccgaaaaa accataaaca atagatggaa
    33001 tggccgccat caagttgata gctgatttta agaagccata gacgggcttt ggacaataat
    33061 aaaccataaa caccgatgtc aagatgcctg ttggcacccc aatcacaatc gctcctaagg
    33121 taattaataa ggaaccaacg atcattggta aaataccata gcttgccgga atgttcgttg
    33181 gcgaccaatc actgcctaat aaaaaacggg caaagccgta gttagccatg aaaggtaagc
    33241 cattactaaa aataaagaaa cagattagca aaatagctac aacagctact gttgcactca
    33301 tgaaaaaaat tgccctaaaa actgcttctt tgaaggcttg ttttgtcaca tcttgtcctt
    33361 tctagtgaag aaagtaaggg agatacgaca cctccctact tgccttcttt atcttattgt
    33421 acgatgaaac gtctgcatct ctttagagat ttatggagca aacattttat ttaatcttgt
    33481 cccaggtggt taatttgcca ctaaaaacgt ctgcaagttc agccatactg acttggcttg
    33541 ccttattgtc attattgacc acaacagcaa taccgtctaa agcaatagca tcatgggtga
    33601 gactcttacc ttcttcagga gttaattccc tagaaaccat accaatatca gcggttttct
    33661 ccttaacagc ggtaatacct gctgaagacc cattagaggt aatatcaatc gtaacttctg
    33721 gattttcttt tttataagct tctgctaatt tttccattaa agaagatact gaagtggaac
    33781 ctacaacaga caacttgcct gataagtgtt ggcttgtata ttctgtggtt tcggttttag
    33841 cttcaataaa tttattatct gtgaccactt gttgaccttg tttggagtgg ataaagctga
    33901 taaaatcttg acctagcttg gaaagattag aagaccaaac aatgttgaag ggacgttgaa
    33961 gagggtattc accatctaaa actgtgtctc gactagcctt gacaccatca atctctaaag
    34021 ccttgacaga tttcgttaaa gatcccaagg agatgtagcc gatagcatta gcattccctt
    34081 gaactgctga gagaacacct tctgtactat tttgaatcac agctgttttg gcagtgttgt
    34141 caattttttt atcaccgtct tttttgagaa tccctgtgat ttctgtgaag gcaccccgtg
    34201 ttccagagcc attttctcgt gaaatcacct caatcgttcc tgaagctgac tgtttggaag
    34261 cagctgactg attgccacag gcaacaagcc caaatcctga taagccaatg gctgcaagag
    34321 taagcatttt tttgaatttc ataataatca cctttatctc tatgtatttt tcttgtgtag
    34381 gcttactaca tttatagtct aacaagtctt tgtaaaggtt tatccctgat tcatgtaaag
    34441 attgtgtaaa gaatcaaaaa aagccacttt tgaaaaatgg ctgctcctaa aaatagcttt
    34501 aaaaattatt agtcctgtgc gaaagattgg ttaggaagaa aaatcgtgaa gcaactgcct
    34561 ctgccaagct gactcgtcac cgtgacttgg ccacctaata attgactgag ttctttgaca
    34621 atggcaaggc caagaccagt gccaccagtt tgtctgcttc gacctttatt aactcggtaa
    34681 aaacgttcaa aaatacgatc ctgctctaat tgactaatac caatccctgt atctgataca
    34741 gaaatcttaa tgccttcgtt caccttttgg gtcttgacct caatttttcc cccttgttca
    34801 gtgtaacgga tggcattgga taaaagattg agtaagattt gggaaagtaa ttgactatct
    34861 gatacgaggg tgacatcatc tggcacctgc acctttagct gtaaatcctt cttcttgagc
    34921 tgaggttgca agctttgagt caaatcctgt acaaattctg ccaaagaaag ggtcgtccat
    34981 tgtataggca tttgttgagc cttagataag gtaagaagat gctcaacaat atgctcaaga
    35041 cgcaagcttt ctttgtaaat aatgtctaga aagtcatcct tgagcgcttc ttcttcagct
    35101 gacatcccct taatggtttc agcaaagccc ttaatcgaag taactggtgt cctcaattca
    35161 tgggaggcat ttgagacaaa ggctaaattt aacttttcat aagttctaat cgttgttaaa
    35221 tcatatagca agacgagcac agcttccaca gattgggtgg ggctaaaaac gggaactgct
    35281 gtcacttcta aaatcaagtc accctcatga aacccactta cttcttgttt taaccttgtt
    35341 ttttgatcaa aggcttggtg aactaaattt cgaatatcca tccgtttgag gtcatcaagt
    35401 gaacttatgt cgccgtccac atcgggaaaa taatgaggca gagagcgact ggataataac
    35461 atctgacctt gagcggaaac taaaaacgtc cccatggtta ggtgcgacag aagaacctcc
    35521 attgtttcgg ctaggtcctt gtattgctga tcctgttggg agactttggt ttttagggca
    35581 gacacatact gagccaaaga ctttaagtct tcttgccctt tttctaaaaa gtattcacta
    35641 ctggtcaaga gaggttggtg caaggtctca aaagcaactt cccatttcca aaggcaaaag
    35701 agccagtagc caccttgtcc caaagaaagg gctagaagaa agagaccgat gcctttactg
    35761 atccaagtta atgccatccc tgcaatcaga atgaggctaa cacttagatt gactagccaa
    35821 aattgaaggt agcgtttcat ctataactcc ttgaacttat aaccataacc ccgaatggtt
    35881 cgaataaatt gaggggcttt aggattgtct tcaatttttt ccctcaactt accaatatga
    35941 acgtccacca aacgtgtttc ctgcccaaag tcataccccc agatacgttc caaaagacgc
    36001 tctctagtca gtgtcatgtt gggatgtttc ataagataga gcaagagttc aaattctttt
    36061 ggggtcaaac tcagtaactt attcgccttg tagacttcat gacgctcagg gtatactttc
    36121 aaggtcccaa atagccaaga atcgtcagcg atattatctg aatcatctcc ttcttgttct
    36181 cctttagttc gcctgaggac agccttgaca cgcgccagca attctctagg gctaaaaggc
    36241 ttggtcaggt agtcatcagc ccctaattcc aaggccaaaa ccttatcaaa ttcatcactt
    36301 ttcgcagaaa ccatcataat tggagttttg acgcctttgg ctctcagccg cttacaaact
    36361 tccatgccat ctaattgtgg taacatgata tcaagcaaga taaaatcaaa gggttctgtt
    36421 tctgccaaag ctaaggcctt ccgtccattt gttaccaatt gagtagaaaa gccttcctta
    36481 cttaaatggt agtcaagcaa tttcagaatg tgttcttcat catccactaa taagacttgt
    36541 tttgtcatct attatctcct attggtaaca ttataacaca attatcagaa atcctaacat
    36601 tgctaaatca gattaaattt gcttatcaag actagtatct ggtcaaacgc tcaatcatct
    36661 ccttgtgctc tggataggtc gccagtagat ctaccctttc aaataattca aaatcctcaa
    36721 attcaaaacc aggagcaaca agacaagaaa ccagagcata atccttatca actgttgatc
    36781 cccaaatagt gcccttagga acacagtagt gaagttgttg ccctttggat atgtccaggc
    36841 ctaaagtgac tgcttcgtag tgaccatctg ctgtaatcat gtgaacagta agtggggatc
    36901 ctgcatgaaa ataccagatt tcatctgctg tcaatcggtg aaaatgtgaa ggattcgttt
    36961 cttctaataa gaaataaata ctggtataaa gcgcccttcc cttaccagca aggtttatag
    37021 tgtctgaagc tttttttgtt tgtctaaaat agccaccttc aacatgggga gctaactcta
    37081 gagttcttat caagtcttct ttatccgtcg gagccaatgg gttgaagtaa ctcttgttca
    37141 aaggtggtgt tacgatttca agaactcctc tcagctctga ggacacgata atgattgatg
    37201 cgacggaagt acaaatcaat cgccctaaaa aaagaattag cgaattattc tggtaaaaaa
    37261 atgcc
//
LOCUS       DAVBBS010000016        34347 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVBBS010000016 DAVBBS010000000
VERSION     DAVBBS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34347
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.016"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..4786
                     /locus_tag="VQU32_000776"
     CDS             <1..4786
                     /locus_tag="VQU32_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2472257.1"
                     /translation="QGERGLTGAKGEKGDQGERGLTGAQGEKGDRGERGLTGAKGEKG
                     AQGQAGRDGVTPTVTVKDNKNDGTHTITINDGRGNVTSTVVRDGFDGASPLVATQRND
                     ADKTTTVIFYYDKNGNNELDASDKKLKEVVIADGAKGEKGDKGEQGLQGRDGEQGPKG
                     EDGKTPTVKVTDGQDGTHTITINDGKGGITTTVVRDGFDGASPLVSTHRNEADKTTTV
                     IFYYDLNDNNQFDEGDTKLKEVVIADGKQGPKGDKGDNGKDGFTPEVTVTDNNNGTHT
                     ITITQPDNRPSLTTIVKNGEDGKTPKVKAERDDAKKQTTLTFYIDKDGDGSYTAGKDE
                     LVQTTVVKDGQDGAAGASGRDGKEVLNGKVDPTTEGKDGDTFVNTQTGDVFVKKGNTW
                     EPAGNIKGPKGDKGADGAKGEKGAQGERGLTGAQGVKGEKGDQGERGLTGSKGEKGDQ
                     GERGLTGAQGAKGDKGEQGLQGRDGAQGPKGADGQRGPAGPQGPKGEQGNPGTPGKDG
                     KSLIAVKNGTETKVYVEDPARPGQPLNPNQPLATITDGRNGTNGQSPTITATRSVQNG
                     KNGVLVTITPVGGRPQTTFVEDGQKGADGKTPTVTITEGQNGTHTLTVHNPGSPDVTT
                     TIRDGATGQAGRDGKDVLNGKVNPQPNQGKNGDKYINTETGDVYVKNNGNWDKEGNIK
                     GPKGDKGADGAKGEKGDQGERGLTGAQGAKGADGAVGRDGRDGKDVLNGKANPEAHQG
                     KDGDKYVNTETGDVFVKNNGNWDKEGNIKGPKGDKGERGEDGKTPEVTVTPGKDGHST
                     DITFTVPGKDPVTVNVKDGENGLNGKTPKVDLLRVQGKNGNPSHTIVTFYTDENNDGK
                     YTPGTDELLGSEMIKDGAKGADGRDGKSLLTVKDGKETKVYQEDPANPGQPLNPEKPL
                     AVIRDGVDGKSPTVTAVRKDEAGHKGVEITVDNHDGSQPTTVFVQDGAKGETGATGQD
                     GQTPTITTQRGQDGQSTVVTITTSGKDPVTFTVKDGKNGKDGRAPKIKVEDITSPSRI
                     RRDTDAAATPTRNGIRVTVYDDVNDNGVYDEGVDKVLNSKDIYNGIDGRDGSAPTITT
                     KDNGDGTHTITVQNPDGSESTTVVKDGKDGKTANITTTENPDGSHTITVTNPDGSTKE
                     TVVKNGKDGKTPKVEVTDNNDGTHTVKVTDGDGNVTNAIIKDGKDGKAATATTTENPD
                     GSHTVTITNPDGTKNEFVVKNGRDGVDGRTPTASVRDNGDGSHTIVITNPEGVTTETT
                     VRDGKSPKVTITDEQNGTHKISVLNGDGTTTTTTVRDGREPKLEVIDNNDGSHTIKVT
                     GADGKGTTTTIFDGKSPKANIVDNGDGTHTLTIVDSDGREYKSIIKDGKDGKDSVSPT
                     VTVKNNNDGTHVVTITNPDGSKTEMVIKDGKDGKSPKVSVEDNGDGSHTITIINSDGT
                     VTKTVIKDGRDGRDGRDGKDGKDGKDGKCGCQDKPVTPSNDKPVPPTPNVPTPEVPVK
                     PVPAQPTPNVPTPEVPVQPTPAVPTPEVPVKPVPAVPEQPVVPTPAQPATPVNANPVA
                     PTTGKENRGDKLPETGSQSDYISVLLGSGILLSLYVGRRKEA"
     gene            complement(5023..5571)
                     /locus_tag="VQU32_000777"
     CDS             complement(5023..5571)
                     /locus_tag="VQU32_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2472258.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            5892..6380
                     /locus_tag="VQU32_000778"
     CDS             5892..6380
                     /locus_tag="VQU32_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HET2472259.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            6377..7564
                     /locus_tag="VQU32_000779"
     CDS             6377..7564
                     /locus_tag="VQU32_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2472260.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTVILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            7557..7922
                     /locus_tag="VQU32_000780"
     CDS             7557..7922
                     /locus_tag="VQU32_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2472261.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            7938..9284
                     /gene="asnS"
                     /locus_tag="VQU32_000781"
     CDS             7938..9284
                     /gene="asnS"
                     /locus_tag="VQU32_000781"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET2472262.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            9437..9727
                     /locus_tag="VQU32_000782"
     CDS             9437..9727
                     /locus_tag="VQU32_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET2472263.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            9739..10209
                     /gene="ssbA"
                     /locus_tag="VQU32_000783"
     CDS             9739..10209
                     /gene="ssbA"
                     /locus_tag="VQU32_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HET2472264.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            10241..10480
                     /locus_tag="VQU32_000784"
     CDS             10241..10480
                     /locus_tag="VQU32_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET2472265.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            10775..12175
                     /locus_tag="VQU32_000785"
     CDS             10775..12175
                     /locus_tag="VQU32_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HET2472266.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSYVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDVIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            12177..12536
                     /locus_tag="VQU32_000786"
     CDS             12177..12536
                     /locus_tag="VQU32_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET2472267.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            12590..13339
                     /locus_tag="VQU32_000787"
     CDS             12590..13339
                     /locus_tag="VQU32_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HET2472268.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            13329..13628
                     /locus_tag="VQU32_000788"
     CDS             13329..13628
                     /locus_tag="VQU32_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET2472269.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALFKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            13699..14634
                     /locus_tag="VQU32_000789"
     CDS             13699..14634
                     /locus_tag="VQU32_000789"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HET2472270.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            14721..15233
                     /locus_tag="VQU32_000790"
     CDS             14721..15233
                     /locus_tag="VQU32_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET2472271.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGAVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            15270..15865
                     /locus_tag="VQU32_000791"
                     /pseudo
     CDS             15270..15865
                     /locus_tag="VQU32_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            15966..16160
                     /locus_tag="VQU32_000792"
     CDS             15966..16160
                     /locus_tag="VQU32_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET2472272.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(16206..17651)
                     /locus_tag="VQU32_000793"
     CDS             complement(16206..17651)
                     /locus_tag="VQU32_000793"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HET2472273.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVHRVYGLTFDVGVFLNITPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            17757..19379
                     /locus_tag="VQU32_000794"
     CDS             17757..19379
                     /locus_tag="VQU32_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET2472274.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            19583..21541
                     /locus_tag="VQU32_000795"
     CDS             19583..21541
                     /locus_tag="VQU32_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2472275.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGFLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFATAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            21713..22648
                     /locus_tag="VQU32_000796"
     CDS             21713..22648
                     /locus_tag="VQU32_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2472276.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYAEKSFFDKSQS
                     GELTSAIVNDTSVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMM
                     FIILLLSNISEKYSRRLQEEIGFLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERV
                     QNNSLKSDRVTSFETPFALLFIFATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLL
                     NPISNIANFVTVYSRSKGSSVALENLLAVPKEKFE"
     gene            22673..23325
                     /locus_tag="VQU32_000797"
                     /pseudo
     CDS             22673..23325
                     /locus_tag="VQU32_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            23483..24577
                     /locus_tag="VQU32_000798"
     CDS             23483..24577
                     /locus_tag="VQU32_000798"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET2472277.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            24585..25751
                     /locus_tag="VQU32_000799"
     CDS             24585..25751
                     /locus_tag="VQU32_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2472278.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEIQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            25844..28942
                     /locus_tag="VQU32_000800"
     CDS             25844..28942
                     /locus_tag="VQU32_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2472279.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDSFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLNRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQNIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            28990..29607
                     /locus_tag="VQU32_000801"
     CDS             28990..29607
                     /locus_tag="VQU32_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET2472280.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            29617..30951
                     /locus_tag="VQU32_000802"
     CDS             29617..30951
                     /locus_tag="VQU32_000802"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET2472281.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFSGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            30961..31425
                     /locus_tag="VQU32_000803"
     CDS             30961..31425
                     /locus_tag="VQU32_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2472282.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     DLDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            31422..31916
                     /locus_tag="VQU32_000804"
     CDS             31422..31916
                     /locus_tag="VQU32_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2472283.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            31997..33646
                     /gene="mltG"
                     /locus_tag="VQU32_000805"
     CDS             31997..33646
                     /gene="mltG"
                     /locus_tag="VQU32_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET2472284.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMTDSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDIIS
                     DTSRRSRREGAKPVNPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPIDA
                     NSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQKSM
                     STEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAEAFLA
                     KVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDEML
                     AAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDMPL
                     QSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIESSI
                     NQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            33717..34199
                     /gene="greA"
                     /locus_tag="VQU32_000806"
     CDS             33717..34199
                     /gene="greA"
                     /locus_tag="VQU32_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET2472285.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
BASE COUNT        10715 a         6373 c         7776 g         9483 t
ORIGIN      
        1 ccaaggtgaa cgcggtttaa ctggagctaa aggtgaaaaa ggggaccaag gtgaacgcgg
       61 tctcactgga gcccaaggtg aaaaaggaga ccgaggcgaa cgtggtttaa ctggagctaa
      121 aggtgaaaaa ggagcccaag gtcaggcagg tcgtgacggt gtaactccaa ccgtaaccgt
      181 taaagataat aaaaatgacg gcactcatac tatcactatt aacgacggta gaggtaatgt
      241 tacaagtact gttgtaagag atggtttcga tggcgcaagt ccattagttg cgactcaacg
      301 aaatgacgca gataagacaa caactgttat cttctattac gataaaaatg gaaacaatga
      361 attagatgct tctgataaga aattaaaaga agttgtaatt gcagatggtg ctaaaggtga
      421 aaaaggggac aaaggtgaac aaggtcttca agggcgtgat ggtgaacaag gaccaaaagg
      481 agaagatggg aaaaccccaa cagttaaagt aactgatggt caagatggaa cgcatacaat
      541 tacaattaac gatggtaaag gtggtataac tactacagta gtaagagatg gatttgatgg
      601 tgcaagccct cttgtttcta ctcatagaaa tgaagcagat aaaacaacaa ctgttatttt
      661 ctattatgat ctaaatgata ataatcaatt tgatgaagga gatacaaaac ttaaagaagt
      721 tgttatcgca gatggaaaac aaggaccaaa aggtgacaaa ggtgataacg gaaaagatgg
      781 attcacacca gaagtaacag ttacagataa caataacgga acacacacca tcacaatcac
      841 acaaccagac aacagaccat cattaacaac aatcgttaaa aacggtgaag atggaaaaac
      901 accaaaagtc aaagcagaac gagatgatgc gaagaaacaa acaacattaa cattctacat
      961 tgacaaagat ggagatggaa gttacacagc aggaaaagac gagttagttc aaacaacagt
     1021 agttaaagac ggacaagacg gagctgcagg agcatctgga cgtgatggaa aagaagtatt
     1081 aaacggaaaa gtagacccaa caacagaagg taaagatgga gacacattcg taaatacaca
     1141 aacaggagat gtattcgtta agaaaggtaa cacttgggaa ccagcaggaa acatcaaagg
     1201 accgaaaggt gacaaaggtg cagatggtgc caagggtgaa aaaggagccc aaggagaacg
     1261 cggcctgact ggagcgcaag gtgtcaaggg tgaaaaggga gatcagggtg aacgcggtct
     1321 aactggatct aaaggtgaaa aaggagacca aggtgaacgc ggtttaactg gagcgcaagg
     1381 tgccaaaggt gacaaaggtg aacaaggtct tcaaggtcgt gatggagctc aaggaccaaa
     1441 aggcgcagat ggacaaagag gaccagcggg accacaagga ccaaaaggag aacaaggtaa
     1501 tccaggaact ccaggtaaag atggaaaatc tctaattgct gttaaaaatg gaactgaaac
     1561 aaaagtctat gtagaggatc cggctagacc aggacaacca cttaatccaa accaaccatt
     1621 agcaactatc actgatggaa gaaacggtac taatggacaa tcaccaacaa ttacagcgac
     1681 acgaagtgtg caaaatggca aaaatggtgt attagtaaca attacaccag taggaggtcg
     1741 tccacaaaca acatttgtag aggatggaca aaagggtgct gatgggaaaa ccccaacagt
     1801 aacaataact gaggggcaaa acggcacaca tacattaacg gttcataatc caggaagtcc
     1861 agatgtgaca actacgatcc gtgatggagc tacaggacaa gcaggtcgtg atggtaaaga
     1921 tgtattaaac ggaaaagtaa atccacaacc aaaccaaggt aaaaatggag ataaatatat
     1981 taataccgaa accggtgatg tctatgttaa aaacaatgga aactgggata aagaaggcaa
     2041 catcaaaggc cctaaagggg acaaaggtgc agatggtgct aaaggcgaaa aaggagacca
     2101 aggcgaacgc ggcctaactg gagcgcaagg agctaaaggt gcggatggcg cagtaggtcg
     2161 tgatggacgt gacggtaaag acgtgttgaa cggcaaagct aacccagaag cacatcaagg
     2221 taaagacggc gataaatacg ttaatacaga aacaggcgac gtcttcgtta agaataacgg
     2281 caactgggat aaagagggca acatcaaagg ccctaagggt gacaaaggtg aacgcggaga
     2341 agatggtaag actccagaag taactgtaac tccaggtaaa gatggccata gtactgacat
     2401 tacattcact gttccaggta aagatccagt tacagtaaat gttaaggacg gagaaaatgg
     2461 tctgaacggt aaaactccaa aagttgattt acttcgtgtc caaggaaaaa acggaaatcc
     2521 atctcataca attgtgacat tctatacaga tgaaaacaat gacggcaaat atacaccagg
     2581 aactgatgaa cttctaggtt cagaaatgat taaagatggt gctaaaggcg cggacggacg
     2641 agatggtaaa tcattgctta ctgtcaagga tggtaaagaa actaaagttt accaagaaga
     2701 tccagctaac ccaggacaac cattaaatcc agaaaaacca cttgcggtaa ttagagatgg
     2761 agtagatgga aaatcaccta cagttacagc tgttcgtaaa gatgaagcag ggcataaagg
     2821 tgtagaaatc actgttgata accatgatgg ttcacaacca actacagtct ttgttcaaga
     2881 tggtgctaag ggagaaactg gtgcaaccgg tcaggatgga caaactccta caatcactac
     2941 tcaacgtgga caagatggcc aaagcactgt tgtaactatc acaacatcag gtaaagatcc
     3001 agtaaccttc actgtaaaag atggtaagaa tggtaaagat ggccgtgcac cgaaaatcaa
     3061 agtagaagat attacttcac cttcaagaat tagacgcgat acagatgctg ctgcaactcc
     3121 aacgcgtaac ggtatccgtg ttacagttta tgatgatgtt aatgacaatg gggtatacga
     3181 cgaaggtgtc gataaagtat taaatagtaa agatatttat aacggtatag atggacgtga
     3241 tggttcagct ccaactatta ctacaaaaga taatggagat ggaactcaca ctatcacagt
     3301 tcaaaaccca gatggttctg aatcaacaac agttgttaaa gatggtaaag acggtaaaac
     3361 tgcgaatatc actacaacag aaaacccaga tggaagccac acaattacgg tgacaaatcc
     3421 agatggttca actaaagaaa ctgttgttaa aaacggtaaa gacggtaaga ctcctaaagt
     3481 tgaagtaacg gataacaacg atggaactca tactgttaaa gttacagatg gagacggcaa
     3541 tgttaccaac gctatcatca aagatggtaa agacggtaaa gctgcaacag caacaactac
     3601 tgaaaatcca gatggaagcc acacagtaac aatcactaac ccagacggaa ctaagaatga
     3661 gtttgttgtt aagaatggac gtgacggtgt tgacggacgt actccaaccg catctgttcg
     3721 tgataatgga gacggaagtc atacaatcgt tattacaaat ccagaaggtg tgacaactga
     3781 aaccacagtt cgtgatggta aatcaccaaa agtgactata actgatgaac aaaatggaac
     3841 tcataagatc tctgttctaa atggtgacgg aacaactaca acaactacag ttcgtgatgg
     3901 tagagaacca aaacttgaag ttattgataa caacgatggt tcacacacta ttaaagtgac
     3961 aggtgctgat ggtaaaggaa cgacaactac aatctttgat ggtaaatcac caaaagcgaa
     4021 catcgttgat aacggagatg gaactcatac attaacaatc gtagattctg atggtcgtga
     4081 atacaaatct attatcaaag atggtaaaga cggcaaagat agcgtttcac caactgtaac
     4141 tgttaaaaat aataacgatg gaactcacgt tgttacaatc actaatccag atggaagtaa
     4201 gacagaaatg gtgattaaag acggtaaaga tggtaaatca ccaaaagttt ctgttgaaga
     4261 taatggtgat ggtagtcata caatcacaat catcaattct gatggaactg tgacaaaaac
     4321 agttattaaa gatggtagag atggacgtga tggtcgagac ggcaaagacg gtaaagacgg
     4381 taaagatgga aaatgtggat gccaagacaa accagtaaca ccatcaaatg acaaaccagt
     4441 tcctccaaca ccaaatgtgc cgacaccaga agtaccggtt aaacctgtgc cagcgcaacc
     4501 aacaccaaat gtaccgacac cagaagtgcc agtacaacca actccagctg ttccaacacc
     4561 agaagtaccg gttaaaccag taccagcggt tccagaacaa ccagtagtac caacaccggc
     4621 tcaaccagca actccagtaa atgctaaccc agtagcacca actacaggta aagaaaaccg
     4681 tggggacaaa ttacctgaaa ctggaagcca atctgattat atctctgttc ttttaggtag
     4741 cggtattcta ttgagcctat atgtaggacg aagaaaagaa gcttaatcag ccaaaagcag
     4801 tcactaattt ctcgaaagaa gatgataagt gatagatctc gaatgaaaga atattcttag
     4861 aatgattttc ctatgatgga aaagactgct aaaaaccttg agtggtaacg ctcaaggttt
     4921 ttttggatgg ctgatgtttg tctgaaaatc ttgattgaga aataaaaagc caccctatta
     4981 ggtggctata attgatttag actttctaaa attttctatt ttttactgaa tcgcttcgac
     5041 caagtcttga gcttgttttt caagtgagtt taggactgtt tcttcaagaa ccaattttcc
     5101 gtctgcccag gcagagtcat taacacgtgc agcagtgaaa tcaccaacgc cttgtgtacg
     5161 gataaatggc aagaggtctt tgtagatagc gaaaagttga tcgtgccctg cattggctac
     5221 agatgatacg gtgacaaact tgtcttgaag ggcagaaacg ccacgcgtat cagacaagtc
     5281 aagggcacga gatagccagt caagcaagtt ttttactgta ccagggatag agaagttgta
     5341 gactggagag aaaatccaga tagcatccgc aacgagaact gcttcacgag cagcagctac
     5401 agctggatgt gttggaactt ccaaatcttg gctgaagaga ggaagggctg aataatcaag
     5461 gtagctaact tccgctttac cagcaagtgc tttctcagct tcgagcgcca tttggtggtt
     5521 gaaagaacct tgacgtagtg atccgacgat aaataatact tttttagaca tgatgtgtct
     5581 cctttttctt caaattttga gaactctctc gttgttataa aatacattac aacaaaacaa
     5641 aagaacttgc aagaaatttg ctcagagttt atttgaaaat tgtaaaattt cggaaactct
     5701 agtcatttgt gactgaaaaa gtaagaactg tttcactttc aaaatcggtt ttgtttgaag
     5761 ggagaaatag aattggttgc tcaaagcaca gctttgaggt tgcagataaa gctgacgtgg
     5821 tttgaagaga ttttcgaaga gtattcttat attcacttgt aataaagctt aatagatggt
     5881 aaaatagacg agtgaaaaaa agacaaaaca aagcaaaaaa taatctactg tggcagtatg
     5941 gtctagggat gacgattttg tttgtggtta tcagtgcttc ctttctgtat atggtttctc
     6001 ttagcatgaa accctatcaa acagctaaaa gtgaaggaga aaaattagct cagcagtatg
     6061 caggattaga gcaggccgat caggttgatt tatacaatgg cttggaatct tattacagcg
     6121 ttcttggtcg taataaacag caagaagcac ttgctgttct gattggaaaa gatgatcata
     6181 agatttacgt ttatcagcta aatcagggtg tttcacaaga aaaagcagaa acggtttcta
     6241 aggaaaaggg agctggcgaa attgacaaga ttatctttgg tcgttatcaa gataagccaa
     6301 tctgggaagt caagtcagga tctgattttt atctagtaga ttttgaaaca ggagcattgg
     6361 tcaacaagga gggcctatga aattatccaa ccgtgtttta gaaatggaag aaagtgtgac
     6421 tttggctgct ggagcccgtg ccaaagcact gaaggctgag ggtagagata ttttgtctct
     6481 aaccttgggt gagccagatt tcactactcc caaaaatatc caagatgccg ccattgcatc
     6541 gattcgagat gggcgtgctt ctttttatac agtaacctca ggtctgccag agcttaaggc
     6601 ggcggtcaat agctactttg agcgctttta cggctattct gtagcgtcaa atcaagtgac
     6661 agtcgctgcg ggagccaaat attctctcta taccttcttt atggctgtgg tcaatccagg
     6721 tgatgaagtc atcatcccaa ccccatactg ggttagctat ggagatcagg tcaagatggc
     6781 agaaggcgtt cccgtctttg tttctgctaa ggaagacaat cactttaagg tgaccgtaga
     6841 gcagttagaa gcagctcgca ctgacaagac caaggttttg gtgctgaatt cgccatctaa
     6901 tcccacaggt atgatttaca cccgtgagga actcttggca attggaaact gggctgtaga
     6961 aaatgatatt ctcatcctag cagatgatat ctatggccgc ttggtttata atggtcatga
     7021 gttcacaccg atttctagcc tatcggaagc gattcgcaag caaacagtgg tcatcaatgg
     7081 tgtgtctaaa acctatgcca tgactggttg gcggattggt tatgccgttg gagaagcaga
     7141 cattatcgct gccatgtcca agattgcagg tcaaacaact tcgaatccgt cagcagtagc
     7201 ccaatatgca gcagttgagg ctctatcagg tgagcaagat actgtagaaa gcatgcgtca
     7261 ggcctttgaa gaacgtctta ataccatcta tcccctcctt gcagaagtgc caggatttga
     7321 agtggtcaaa ccgcaagggg ccttctatct cttcccaaat gtcaaaaagg ccatggagat
     7381 gaaaggctac acggatgtga cagactttac aactgttatc ttagaagaag ccgaagtggc
     7441 cttggtaaca ggagctggat ttggagcacc agaaaatgtc cgcctcagct atgcgacaga
     7501 cctagacacg cttaaagaag cagtcgaacg cttgaaagca tttatgggta gtgagaatga
     7561 ttgatcattt tgagattaag gtaaaggatt tacaaatttc agaaggattt tataggagtt
     7621 ttctcgctcc tttggactat aaattggctt ttaagactag ttctctaatt agttttctat
     7681 ccccgaacag ccctcatcct ggtggtgatt tttggctgac tcagggaaca caagatcctg
     7741 ttcattttgc ttttttagca gaaaataagg aagaagttca ggcttgttat gaggctggct
     7801 tagaggcagg tgggcgagac aatggggctc ctggttatcg aagtgagcat ccgatttact
     7861 atgctgcttt tatgattgac ctggatggga acaatataga ggtggtttgc cataaagaat
     7921 aaaaaagaaa aggaaaaata atgacaaaac gtgtaacgat tattgacgta aaagactatg
     7981 ttggtcagga agtgacgatt ggcgcttggg ttgccaacaa atcaggaaaa gggaaaatcg
     8041 ctttcttaca attgcgtgat ggaacagcct tctttcaagg tgtggctttt aaaccaaact
     8101 ttgtcgaaaa atttggtgaa gaagtgggac ttgagaagtt tgatgttatc aaacgcttga
     8161 gccaagaaac gtctgtttat gtgacaggta ttgtcaaaga ggacgaacgt tctaaatttg
     8221 gctatgagtt ggacatcaca gacatcgaag tgatcggtga atctcaagac tacccaatca
     8281 caccaaaaga acacggaaca gactttttga tggataaccg tcacttgtgg ctacgctctc
     8341 gtaagcaagt agctgtgttg caaatccgta acgctattat ctatgcaact tatgagttct
     8401 ttgacaagaa cggttttatg aagtttgaca gcccaattct ttcaggaaat gcggcagaag
     8461 attctacaga actctttgaa actgactact tcggaacgcc agcctacttg agccaatcag
     8521 gtcaacttta cctagaagca ggggctatgg ctcttggtcg tgtctttgac tttggtccag
     8581 ttttccgtgc tgaaaaatca aaaacacgcc gtcacttgac tgagttctgg atgatggatg
     8641 ctgagtactc atacttgaca catgatgagt cgcttgactt gcaagaagct tatgtgaaag
     8701 ctcttctaca aggtgttctt gaccgcgcgc ctcaagcctt ggaaaccttg gaacgtgata
     8761 cagaactctt gaaacgctac attgcagagc cattcaaacg tatcacttac gatcaagcca
     8821 ttgacctctt gcaagagcat gaaaatgatg aagatgctga ctacgagcat cttgagcatg
     8881 gtgatgactt tgggtcacca cacgaaactt ggatttcaaa ccactttggt gtgccaacat
     8941 ttgtcatgaa ctatccagca gccatcaagg ccttctacat gaaaccagtt cctggaaatc
     9001 cagagcgcgt gctttgtgca gacttgcttg ctccagaagg ctatggagaa attatcggtg
     9061 ggtctatgcg tgaggaagat tacgatgccc ttgtcgctaa gatggatgaa cttggcatgg
     9121 atcgtacaga atatgaattc taccttgacc ttcgtaaata cggtacagtt ccacacggag
     9181 gatttggtat cggtatcgaa cgtatggtaa ccttcgcagc aggaacaaaa catatccgtg
     9241 aagctattcc attcccacgt atgttgcacc gtatcaaacc ataagaacag gaaaacgccc
     9301 atgtggcgtt ttttcgaatt ctagtgtttt tacttgcgta aaaaaaattt ttctagtata
     9361 atagtttatt gtgagcgaac ctcacttacc ccttgcaaag tcttggggtc attagaccaa
     9421 aaggaggaac atatcaatgg ctaaatacga aattctttat atcattcgtc caaacattga
     9481 agaagaagct aaaaacgctt tggtagcacg ttttgactct attttgactg acaacggtgc
     9541 aactgttgtt gaatcaaaaa cttgggaaaa acgtcgtctt gcatacgaaa tccaagattt
     9601 ccgtgaagga ctttaccaca tcgttaacgt tgaagcaaat gacgatgcag ctcttaaaga
     9661 gtttgaccgt ctttcaaaaa tcaacgctga cattcttcgt cacatgatcg tcaaaattga
     9721 cgcgtaagaa ggtaaactat gattaacaat gttgtacttg tagggcgtat gacacgtgac
     9781 gctgaattgc gttatacccc atcaaatgta gcagttgcga cttttactct tgcagtaaac
     9841 cgtacattta agagtcaaaa tggtgaacgt gaggctgatt ttatcaatgt cgttatgtgg
     9901 cgccaacagg ctgaaaatct tgctaactgg gctaaaaaag gctcacttat cggggtgaca
     9961 ggtcgtatcc agactcgtag ttacgataac cagcaaggac aacgtgtcta cgtgacagag
    10021 gtcgtggctg agaatttcca aatgttggaa agccgtagtg tgcgtgaggg tcacacaggt
    10081 ggagcttact ctgcaccaac tgcaaactat tcagcaccta caaattcagt accagacttt
    10141 tcacgtaatg aaaatccatt tggagcaaca aacccattgg atatttcaga tgatgattta
    10201 ccattctaat ggacaactaa acttataaag gagaaaaaac atggctcaac aacgtcgtgg
    10261 cggattcaaa cgccgtaaaa aagttgatta catcgcagca aacaaaattg aatatgttga
    10321 ttacaaagat actgagcttc ttagccgttt cgtttcagaa cgtgggaaaa tccttcctcg
    10381 tcgtgtaaca ggaacttcag ctaaaaacca acgtaaagta acaacagcta tcaaacgcgc
    10441 tcgcgtaatg gctttgatgc ctttcgtaaa cgaagattaa agaaaagacc cggttgggtc
    10501 tctttttcat gaacttgaaa acaagaaagc gaattaagac cccaacgggt cttttttcac
    10561 aagctttgaa atgagagagc gagttaggct cagtggctcc ctttttacga gtttgaaaac
    10621 aagagaacac attaaaagca cgatggagct ttttcataaa tatgtctttt ttttcatcca
    10681 agaaattaaa ataaaaatga taaacttttt gaaaataggg gatttgccta ctttatgata
    10741 taatggaaag agttagattg aaagaggtag tgagatggac gcgaaattaa gatacaaggc
    10801 aaagaagatc aagattgtct tttttgatat tgatgataca ttgcggaatt caaagacagg
    10861 gtttattcca actacaattc ccactgtttt taaacagttg cgtgaaaaag gaattttaac
    10921 aggaatcgcc tctggacgtg gcatttttgg tgttgttcca gagattcgtg atctcaaacc
    10981 tgactttttt gtaactttga atggggctta tatcgaagat aaaaaaggtc aggtcattta
    11041 tcagcatcag attgaaaagt catatgttga ggagtatatc tcttgggcta agcaagaagg
    11101 aattgagtat ggcttggttg ggagtcatga tgccaagttg tcgactcgca ccgatatgat
    11161 gagtgaagct atcaatccaa tttatcccga cttagatgta gatcccgatt tccatgaaaa
    11221 agaagatatc tatcagatgt ggacttttga agataaggga gatgacttgc acttgcctga
    11281 cagtctctca gacaaacttc gcatggttcg ttggcatcaa cattcgtctg atattgtgcc
    11341 gatttcaggc tccaaagcga cgggggtgga aaaggttgtg gaacaccttg gcttgaaacc
    11401 agagaaggtc atggtttttg gagatggtct caatgacttg gaactctttg attatgctgg
    11461 aatcagcgtt gccatgggaa tttctcatga taaaatcaaa gaaaaagcag attatattac
    11521 aaaaacatta gaagaagatg gcatttttga tgccttagaa gtatttggta tggtagaaaa
    11581 agaattgcat tttccacaag tagacattga aacagtagaa ggtcctcttg cgactattaa
    11641 gaccaatcac ggagacttac gtatcaagct tttccctgaa catgctccta aaacagtggc
    11701 taactttgta tctctttcaa aagatggcta ctatgatggt gtcattttcc accgtattat
    11761 caaggacttt atgatccaag gtggagaccc aactggaact ggtatgggtg gcgagtcaat
    11821 ctacggcgaa tcttttgagg atgaattctc agaagagctt tacaatatcc gtggtgctct
    11881 ttccatggca aatgctggtc caaataccaa cggcagccag ttctttatcg tgcaaaacca
    11941 acacctacct tattctaaga aagaaattac tcgtggtggt tggccagaac cgattgcaga
    12001 aatctatgcc aatcaaggtg ggacacctca cctagaccgc cgtcacacgg tttttggtca
    12061 gttagctgat gaagcatctt acgctgtctt ggatgtcatt gctgctgttg agacaggagc
    12121 tatggacaag ccagttgaag atgttgtaat tgaaactatt gaaatcgagg actaagatga
    12181 aaatcggtga taagctaaag gggcgtatta cagggattca gccctacggt gcctttgttg
    12241 agttagagac gggtgatacg gggctgattc acatttcaga aattcggaca ggatttattg
    12301 aaaatattca tgaaaccttg aaagtcgatg aagaggttca agttcaggta gtggatttag
    12361 atgaatttac ggggaaagcc agtctttcta tccgcacttt ggaggaagaa aagtaccagt
    12421 ttcctagacg gagacgcttt tcaagcgacc gctttaacta cggttttgct ccctttcgac
    12481 gaatgctacc aatctggact ggagaagccc tgcaccattt aaaaaagaag aagtagttta
    12541 gagttttata ttttttgtaa tgttaatata aatttgctat aataggacca tggaagaaga
    12601 acaattatta aaatcaggag agcgcattaa ccagctcttt tcgacagata tcaaaatcat
    12661 tcaaaataga gaggttttta gctattcggt ggatagtgtt ctcttatcac gatttccacg
    12721 ttttcctaag aaggggttga ttgtggattt ctgtgctggg aatggagcag tggggctttt
    12781 tgctagcact cgtactcaag cacagatatt gtctgttgag attcaggagc gtttggcgga
    12841 tatggctgaa cgctctgtcc gtttgaatgg attagaggag caaatgcagg tcatctgcga
    12901 tgatttgaaa aatatgcctg ctcacattca ggggagtaag gtggatatga ttttgtgtaa
    12961 tccaccctat ttcaaggtgg atccttattc taatctgaac gagagtgaac attatctctt
    13021 ggctcgacac gaaatcacga ctaatttgga agaaatctgt catagtgccc agagtattct
    13081 caagtctaat gggcgtttgg ccatggttca tcgtcctgat cgacttctgg atattttgga
    13141 tacgttaaaa cgacataatc tagcccctaa gcgcctgcag tttgtttatc caaaaagaga
    13201 aaaggaagcc aatatgcttt tgattgaggc gatcaaggat ggctcaacaa gtggctttaa
    13261 ggtcttacct cctttgattg tccacaatga tgatggctct tatacgcccg aaatcgaaga
    13321 gatttattat ggatcataag gcttatatgt acgttctgga gtgtcgtgat ggatcctact
    13381 atataggcta tacgactgat atgagaagac gcctcgctat ccacaatagt gggaagggag
    13441 ccaaatatac acgagcacgc ttgccagtca aacttatcta tgctcaaggt tttgccagta
    13501 aggaagaagc catgtcggct gaagctcttt tcaagcgtaa gaagaggcca cagaaggaag
    13561 aatttttatc tgaaaatcaa gatagaaatt tactcagtta ttttgaagaa tcgtggggag
    13621 tcctttgact cctcttacct ttgtcaaaaa gcgaaaaaaa tgctacaata aagaaaataa
    13681 acaaaatgag gtattatcat gtccaagatt ctagtatttg gtcaccaaaa tccagactca
    13741 gatgccatcg ggtcatctgt agcttttgcc taccttgcaa aagaagctta cggtttggat
    13801 acggaagctg ttgcccttgg aactccaaat gaagaaacag cctttgtctt gaactatttt
    13861 ggtgtggaag caccacgtgt tatcacttct gccaaagcag agggggcaga gcaagttatc
    13921 ttgactgacc acaatgaatt ccaacaatct gtatcagata tcgctgaagt agaagtttac
    13981 ggtgttgtag accaccaccg tgtggctaac tttgaaactg caagcccact ttacatgcgt
    14041 ttggagccag ttggatcagc gtcttcaatc gtttaccgta tgttcaaaga acatggtgta
    14101 gctgtgccta aagagattgc aggtttgatg ctttcaggtt tgatttcaga tacccttctt
    14161 ttgaaatcac caacaacaca cccaacagat aaaatcattg ctcctgaatt ggctgaattg
    14221 gctggtgtaa acttggaaga atatggtttg gcaatgttga aagctggtac caacttggct
    14281 agcaaatctg ctgaagaatt gattgacatc gatgctaaga cttttgaact caacggaaat
    14341 aatgtccgtg ttgcccaagt gaacacagtt gacatcgctg aagttttgga acgccaagca
    14401 gaaattgaag ctgcaatgca agctgccaac gaatcaaacg gctactctga ctttgtcttg
    14461 atgattacag atatcgtcaa ctcaaactca gaaatattgg ctcttggtgc caatatggac
    14521 aaggtcgaag cggctttcaa tttcaaactt gaaaacaatc atgccttcct tgctggtgcc
    14581 gtttcacgta agaaacaagt ggtacctcaa ttaactgaaa gctttaatgc gtaagatttt
    14641 gggtgtcagc tcaaaatcgg aaagtctagt ttgccttata tcgaaaggag tttcggctcc
    14701 tttttttcta ggagtgaagt atgttagaaa atggcgattt gatttttgtg agagatgggt
    14761 cagacatggg acaggccatc cagacttcca caggtaacta tagccatgtt gccatttatt
    14821 tggatgggat gatttatcat gctagtggac aggctggtgc tgtctgtcaa gaaccggcag
    14881 acttctttga gtccaatcat ttgtacgacc tctatgttta cccagaaatg gatatccagt
    14941 cggtgaagga aagagcttgc agacatcttg gagcacccta caatgcttct ttctatccag
    15001 atgcagctgg tttctactgc tcccagtata tagcagaaat cctacctatt tttgaaacta
    15061 ttcctatgaa atttggagat ggggaacagg agattagtga tttttggagg gagtattaca
    15121 tagaactagg tctgcctgtt cctctgaacc aagctgggac caatcctagt cagttggcag
    15181 catcgcctct gttacaatgt aaagaaagga atcttcatga ttcagatttt taattcatct
    15241 cgtttgacga gacagccatt tttggagaat tgatccgcta tctggaccag tatgaggatg
    15301 tgattctacg ggaaattaag gctcaatttc cagatgttgc agttgataaa ctcatggaag
    15361 agtatataaa ggcaagcttg attctacgtg aaaataagcg ctattacctc aattttccta
    15421 cgcttgaatc acttgatagt cttgaactgg atcaagagat ttttgtcaga gaagctagtc
    15481 cggtctatca agccttgttg gagcagagtt ttgagacgga attgcgcaat caaatcaatg
    15541 cagctatttt agttgaaaga cggactttgc gcgcattaaa atgaccctgt ccaattattt
    15601 ttacaaggtc aaacagcagt atcctttgac agaaaaacag caggagctct atgacatttt
    15661 aggagatgtt aatcctgagt atgcactcaa gtatatgacg gcttttttgt tgaaatttct
    15721 caaaaaagac cagcttatgc agaaatgccg tgatatcttt gtggacagtt tggttgtctt
    15781 aggctatatt gtgcaaaatg aagatagaaa gtatgagttg gctatcgatt ttgataagga
    15841 gagattaact ttctacttag cgtgatttct tgtttctgag cacattgttt gactttcctt
    15901 agtattcggt ataaactata tgtaaccggt aacacatatc ggaataaact aaaggagaca
    15961 atcatatgtc acttgaaaac aaattggaac aagcaacagg cgctgtcaaa gaaggttttg
    16021 gtaaagttac tggagacagc aagacagaac ttgaaggagc tgttgaaaaa acagttgcta
    16081 aggcaaaaga cgttgtagaa gacgcaaaag gtgctgtaga aggtgccgtt gaaggtttga
    16141 aaaacgtttt tactaaataa taggaaaaaa tcaagggttt cattttccct tgattttttc
    16201 tattcttata aataattttc tgcgacggct gtatctcctg ggtaggattc tttcttgccc
    16261 tggatgattt ggtaacaatc ggctccctta cccgcaataa taactgcatc taattcgtga
    16321 tttgtgatag ccattgccgc cttgatggct tcttggcgat ccgcaatctt ttcaacagga
    16381 tgattgatgt agctactaat ttcatctgca atggccattg ggtcttcata gttagggtca
    16441 tcagcagtca gaaagacttg aatctcaggg tgttgattga ggaggaggcc aaagtcctta
    16501 cgacgacttt ctcccttgtt tcctgttgat cccagaacca gagcaatctt tccggtttga
    16561 tgagtttcaa ccacattgat gagttttttc agactatccc cattgtgggc atagtcgatg
    16621 aagaccttgg ctccattttt ctgagtgagg acttccatac gaccaggaac gcgggttgca
    16681 gcgatgcctt ttttgatgtc ctcaagactt gctccgagac ggagacaagc aagtccagca
    16741 gcaactgcat tttcttggtt gaagttgcca atgagttgga tatcataatc tccagcgagt
    16801 ttacccgtag ctgaaaagct aaaggctttg gaattctcga tttggttatc aaattggcta
    16861 ccatagaaat catggtcttg atcttcaacc tgttctttca agactgagaa gtggtccatg
    16921 tcactgttaa tgatgactgc tcggctattt tccatcaaga gacgcttgtg gtagaaatag
    16981 tcttcaaagc tagggtgttc aatcgggccg atatggtcag gagtgatgtt aagaaagact
    17041 cctacatcaa aggtcagtcc atagactcga tggactagat aggcttgact ggagacttcc
    17101 atgatgaggt gggtacggtc attttgcaca gcctgattca tcatgtcaaa gaggtcaata
    17161 ctctcagggg ttgtcaacgc tgacttaaag aaagtctcgc catcaagagt tgtgttcatg
    17221 gtcgacaaca tagcaggtct atgcccttga gataagatgt tataggcgaa ataggttgct
    17281 gttgtcttac ccttagtacc agtaaaggca aggagtttga gtttctcttg tggattacca
    17341 tagaactcca tggcaatcaa actcatggct ttctttatat cgttcacaat gatgacaggg
    17401 atatcgactt cgtagtcctt ttcagctaca taccaagcta aaccttgtgt tatagcagaa
    17461 agaaggtatt cttttttaaa ggcagcgcct tttgcaaaaa aaagagtgtc ttctgttact
    17521 tttcggctgt cgtagctgat gctatcaaaa ataactttgc tgtagttgta gtggtaatga
    17581 ccttggtcaa taatttcgcg aaaaaggcca tctttcttta aaatatctaa tacggtttca
    17641 atcttaatca tactttctat tgtaaaccga aagtcgtaaa tttacaagta acaaggaaaa
    17701 gtttataatg gaagataagg agtttttcct agttatcaaa attgaatgag gaatctatgt
    17761 cgcacgaaaa caatcaccag caggcccaga tgttacgggg gactgcttgg ctaacggcta
    17821 gtaactttat cagtcgccta ctcggggctg tttacattat cccttggtac atctggatgg
    17881 gggcttatgc agctaaggca aatggtctct ttaccatggg ttacaatatc tatgcttggt
    17941 tcttgttggt ttcaacagcg gggattccag ttgcggtggc caagcaagtt gccaagtata
    18001 ataccatgcg agaagaagag catagctttg ccctgattcg gagcttctta ggctttatga
    18061 caggactagg cctggttttt gctttagtct tgtatgtctt tgctccttgg ctagcagact
    18121 tgtctggcgt gggcaaagac ttgatcccaa tcatgcaaag cttggcttgg ggagtcttga
    18181 ttttcccgtc tatgagtgtt atccgaggat ttttccaagg gatgaataac ctcaaaccct
    18241 atgccatgag ccaaattgct gagcaggtca ttcgtgttat ctggatgctc ctagcaacct
    18301 ttatcattat gaagctcggt tcaggagatt atctagcagc cgttacccaa tcaacctttg
    18361 ctgcctttgt cggtatggta gccagttttg cagtcttgat ttatttcctt gcccaagaag
    18421 gttcactcaa aagagtcttt gaaacaggag ataagattaa cagtaagcgt ctcttggttg
    18481 ataccattaa ggaagccatt ccttttatcc tgacagggtc tgccatccag ctcttccaga
    18541 ttttggatca gctgaccttt atcaatagta tgagctggtt taccaactac agcaatgagg
    18601 acttggttgt catgttttct tatttctcag ccaatcctaa taaaatcacg atgattttga
    18661 tttctgtagg ggtttcgatt gggagtgttg gtttgccact tttgacggaa aactatgtca
    18721 agggggactt gaaagcagct tctcgtctcg ttcaggacag tctcacccta ctctttatgt
    18781 tcttgctacc agcaacggtt ggagtggtta tggtaggaga acctctttat acggtcttct
    18841 atggtaagcc agatagtttg gctctgggct tatttgtctt tgcagttttg cagtctatta
    18901 ttttaggctt gtacatggtc ttgtctccaa tgcttcaggc catgttccgc aaccgcaagg
    18961 ccgttctcta ttttatctat ggttctattg ccaagctagt cttgcaacta cctaccatcg
    19021 ccctcttcca cagttatggt cctttgattt caacaaccat tgctctcatc attcctaacg
    19081 tcttgatgta tcgggatatt tgtaaagtaa ctggtgtcaa gcgcaaggtg attttgaagc
    19141 gaaccatttt aatcagtttg ctgaccctag tcatgtttct gttaatagga accatccagt
    19201 ggctgttagg atttttcttc caaccaagtg gacgtttgtg gagcttcttt tatgtagctc
    19261 ttgtcggtgc catggggggt ggactttata tggttatgag tctgcgtacc tatttattag
    19321 ataaggtaat aggaaaagcc caagcagatc gcctgcgagc aaaatttaag ctttcgtaag
    19381 gtagcattta taaataaaag gaataagttg aacaatcttt taggagaact tgaagcttat
    19441 tcctttttta gcgtaaaaat agctaaaatt atatttcaaa aatagaaaat gaggcttttt
    19501 tcgatttttt ggaaaaaatc attgactaaa caaactaaac gttgtataat aaaacaaata
    19561 tttaaagcag gaggttccgg aaatgaaaaa gtctaagagc aaatatctaa ccttggcagg
    19621 tcttgtcctg ggtacaggag ttttattgag cgcgtgtgga aattctagca cggcgtcaaa
    19681 aacatacaac tatgtttatt caagtgatcc atctagcttg aactatctag cagaaaaccg
    19741 cgcagcaaca tccgatattg ttgcaaattt ggtagacggg ttattagaaa atgaccaata
    19801 tgggaatatt attccatcat tagcagagga ttggactgtt tctcaggacg gtttgaccta
    19861 tacctacaaa cttcgtaagg atgccaagtg gtttacttct gagggagaag aatatgcgcc
    19921 tgtaactgcc caggattttg tgacaggttt gcaatatgca gctgataaaa aatcagaagc
    19981 cttgtatcta gtgcaggact ctgttgctgg tttggatgac tatatcactg gtaaaacaag
    20041 cgacttttca actgtcggtg tcaaggcact tgatgaccaa acggttcaat atactttggt
    20101 taaaccagaa ctttactgga attcaaaaac acttgcaacg atactttttc ctgttaatgc
    20161 agatttcctg aaatcaaaag gggatgattt tgggaaggcg gatccatcta gtattttgta
    20221 caatggacct ttcttgatga aagcacttgt ctcaaaatct gctattgaat ataagaaaaa
    20281 ccctaattac tgggatgcta agaatgtctt tgtagacgat gtgaaattga cctactatga
    20341 tggtagcgac caagaatcac tggaacgtaa ttttacagct ggtgcttata ctacggctcg
    20401 tctttttcct aacagctcca gctatgaagg gattaaagaa aaatacaaaa acaatatcat
    20461 ctatagtatg caaaattcaa cttcatattt ctttaatttt aacctagata ggaagtctta
    20521 caattatact tctaaaacaa gtgacattga aaagaaatcg actcaggaag cagttctcaa
    20581 taaaaacttc cgtcaggcta tcaattttgc ttttgacaga acatcttatg gggctcagtc
    20641 tgaagggaaa gaaggtgcaa caaagatttt gcgtaaccta gtggttcctc caaactttgt
    20701 cagtatcaag ggaaaagact ttggtgaagt tgtagcctct aagatggtca actatggtaa
    20761 ggaatggcaa ggtatcaact ttgcggatgg tcaagaccct tactacaatc ctgagaaagc
    20821 caaggctaag tttgcggaag ctaagaaaga actcgaagca aagggtgttc aattcccaat
    20881 ccacttggat aagactgtgg aagtaacaga taaagtaggc atacaaggag ttagttctat
    20941 caaacaatca attgaatctg ttttaggttc tgataatgta gtgattgaca ttcagcaatt
    21001 aacatcagat gagtttgaca gttcaggcta ctttgctcaa acagctgctc agaaagacta
    21061 tgatttatat catggcggtt ggggacctga ttatcaagac ccgtcaacct atctcgatat
    21121 ttttaatact aatagtggag gatttctgca aaatcttgga ctagagcctg gtgaggccaa
    21181 tgacaaggct aaggcagttg gactggatgt ctatactcaa atgttggaag aagctaataa
    21241 agagcaagat ccagccaaac gttatgagaa atatgctgat attcaagctt ggttgattga
    21301 tagttcttta gttcttccaa gtgtttcgcg tgggggaaca ccatcattga gaagaaccgt
    21361 accatttgct actgcctatg gtttaaccgg tacaaaaggg gttgaatcat ataaatacct
    21421 caaagtacaa gataagattg tcacaacaga cgaatatgca aaagccagag aaaaatggtt
    21481 gaaagaaaaa gaagaatcca ataaaaaagc ccaagaagaa ttggcaaaac atgtcaaata
    21541 aaatctaatt gtagataagt ttgtgtaaga agtctttgtg cttaaaaacg cataatatca
    21601 ggtatataaa atcctagatg ttatgcgttt tgttatagga agatttatta gattttatcc
    21661 tcaaattgag ttattcccga ctttatccgt tgaaatatta taaggagtat ttatgaaatt
    21721 aaaattatta agagtagata ctaaggtgat tatggggagt ttcttacttg ttctgtctag
    21781 tctacttgct ttgttgcttc cccttatctt aaagggttta atagatggga gttctattga
    21841 aaatataggt tccaaagtat ttcaatcgtt tttgattttt attggtcaag ccttgttttc
    21901 ttctattggt tactatctgt ttagtcaatc gggtgaaaaa aagatagcaa aaatcaggaa
    21961 aaaagtgata gaggggttga tttatgcaga gaaatccttc tttgataaga gccaaagtgg
    22021 ggagttgact tctgccattg tcaatgacac gagtgtcatt cgtgagtttt taattacgac
    22081 tttcccaaat attattctga gtttagttat ggtacttggt tccattgtag tcttatttag
    22141 tcttgattgg aatctttctc tacttttatt catcactctt ccttgtatga tgtttattat
    22201 cttgctcctt tccaatatca gtgaaaagta tagtcgtcgt ttacaggagg aaatcggatt
    22261 tttaacaggg caattgactg aaaagattca agaacatgaa ttaattaaga ctaaccaagc
    22321 agagaaaagt gtacaggatg ttttggataa ttgtattgaa agggtacaaa acaattcact
    22381 gaaatcagat agggttactt cttttgagac cccgtttgct ctcttattta tctttgcgac
    22441 tatagctgtg atgctaacct atgggggtta tcgggtcagc gcaggatata tatctgtggg
    22501 aaccttggtt tcgtttttga tttacctctt tcaattactt aatcctatta gtaatatagc
    22561 taattttgta actgtttatt ctaggagcaa gggatcttca gttgcactgg agaacttgct
    22621 tgcagttcct aaagaaaaat ttgagtgagg aaaatcggta tcaggacaag ggttgaattt
    22681 taaccatgtc tattttggtt atgatgaaaa tcgacctgtc ttaaaggata ttacttgttc
    22741 aattttcaag gggcaaaaaa ttgcttttgt tggaccatct ggatcaggaa aatcaacgat
    22801 tgtgcgtttg ttagagcggt tttataaacc gctttcagga gatattctaa tggagcaatc
    22861 aagtatatat gattttaact taaaagaatg gagaagtaaa atcgcttagt aactgatgat
    22921 gaattgatga aagtgctaga cttagtatca ctaggtgatg agattcgctc catgaaagag
    22981 ggactagata ctgaagttgg tgaacgcgga cgactcttgt caggggggca aagccaaaga
    23041 cttcaaatag ctagagccta cttaaaagat gctgaaattc ttatatttga tgaagccact
    23101 gctaatcttg atgcggattc tgagtatgcg attatcagta gcctctattc tgtattaaag
    23161 gagaagacgg ttgtgattat agcgcatcgt ttgtcaacgg taaaagatgt ggattgtatt
    23221 ttcttcttag aggaggggaa aatcactggc tcaggaactc ataaggaact actggaaaat
    23281 catgagcgtt atgctcgttt tgtgcaggag caaatgatag agtgaaggtg tcttttgaga
    23341 ttcaccattt tatagtctat taaagggagc aggaaaaact ccctttttat atagtttgaa
    23401 actataacta gctcttgaaa agaagaaaat gagttgatga aaataagtgg tacaatagtt
    23461 actatagatt tggaggtatt gtatgagcaa ggaattacac attaacacaa ttttggccca
    23521 ggcgggtatt aagtcagatg aagcgacagg tgcattggtg acaccgcttc atttttcaac
    23581 gacctatcag catccagagt ttggtcgatc tactgggttt gactatacgc gcactaaaaa
    23641 tccaactcgt agtaaggctg aggaagtctt ggcggctatt gagtcagcag actatgccct
    23701 agcgactagc tcagggatgt cagctattgt actggccttt agcgtctttc cagtaggaag
    23761 taaggtcttg gcagtgcgtg atctttacgg tggttctttt cgctggttta accaagtgga
    23821 gcaggaaggt cgtttccatt ttacctatgc caatacagaa gaagagttga ttgccgagtt
    23881 agaaaaggat gtggatgttc tctatatcga aaccccaacc aatcccttga tgttggaatt
    23941 tgatatcgaa aaactagcaa aattggctca tgctaagggt gccaaagtgg tggtggacaa
    24001 taccttctat agccctatct accaacgtcc gattgaagat ggagcagata tcgttctcca
    24061 ttcagcaacc aagtatctag caggccacaa tgatgtcttg gctggagtgg ttgtgaccaa
    24121 tagtttagaa ctatacgaga agctttttta caatctcaat acaacagggg cagtcttgtc
    24181 tccatttgac agctaccagt tgcttcgtgg tctcaagacc ttatctcttc gtatggagcg
    24241 ctcaacagct aacgcccaag aagtggttgc ctttttgaag gattctccag cagttaagga
    24301 agttctctac actggtcgtg gaggcatgat ttcctttaaa gtagccgatg aaacacgcat
    24361 tcctcatatt ttgaacagtc tcaaggtctt ctcttttgcg gaaagtttgg gcggagtgga
    24421 aagtcttatt acttatccaa cgactcaaac tcatgctgat attccagcag aagtacgcca
    24481 ttcttatggt ttgacagatg acctcttgcg tttgtctatt gggattgagg atgctagaga
    24541 tttgattgca gatttgcgcc aagccttaga aggataagac aaagatggga aaatatgatt
    24601 ttacaagcct gcccaaccgt ttagggcacc atacctataa atggaaagaa acagaaacgg
    24661 atagtgaagt tctaccagct tggatagcgg atatggactt tgtggtcttg cctgaaatcc
    24721 gccaagccgt gcaaacttac gcagaccaac tggtttatgg ttatacctat gccagtgaag
    24781 acttaattaa ggaaattcaa aagtgggaag ctacacaata cggttacaac tttgacaaag
    24841 aggctcttgt ctttatcgag ggtgtggtac cagccatctc aacagctatt caaaccttta
    24901 caaaagaagg cgaggcggtt ttaattaaca cgcctgtcta cccacccttt gctcgcagtg
    24961 tcaagttgaa taatcgtaga ttgattacta attccttagt ggaaaaggat ggtctgtttg
    25021 agattgactt tgaccaactt gaaaaggatt tggtggaaga ggaggttaaa ctctatattc
    25081 tttgcaaccc tcacaatcct ggtggacgtg tttgggaaaa agaagtgttg gagaagattg
    25141 gccaactttg ccaaaaacac ggtgttttgt tagtttcgga tgagattcac caagatttga
    25201 ccctctttgg tcacaaacac cagtctttca ataccatcaa tcctgccttc aaaaattttg
    25261 ctatcgtctt gagcagtgcc actaaaacat ttaatattgc tggaacaaaa aattcctatg
    25321 cagtcattga aaatcctaag ttgagactgg ctttccagaa acgcctgttg gccaataatc
    25381 agcatgaaat ttcaggcttg ggttatttgg cgacagaagc tgcctatcga tacggtaaag
    25441 attggctaga ggaactcaag caagtctttg aagaccacat caattatgtg gtggatctat
    25501 ttggaaaaga gactaaaatc aaggtcatga aaccgcaagg tacctacttg atttggcttg
    25561 acttttcagc ctatgacctg actgatgaaa cattgcaaga gttgttgaga aatgaagcca
    25621 aggttatcct caaccgtggt ttggattttg gagaggaagg aagtctccat gcccgcatca
    25681 atatagctat gcccaaatct ctgttgcaag aagtctgtca gcggattgtg gctacttttg
    25741 ccaaacgtta aaaatccagc cttctaggag aaaagtcttc ctagaaggct attttcatag
    25801 gcgaaaatat ggtataataa aaagataagg taaaggtgaa aatatggcta aattgattcc
    25861 ggggaaaatt cgtatcgaag gtgttgccct ttatgaaact ggtaaggttg atatcatcaa
    25921 ggaaaagaac aatcggctct acgctcgcgt tgcagaagaa gaactgcgct atagtttaga
    25981 ggatgatttg gttttttgtg cctgtgattc ttttcaaaag aggggctact gtgtgcattt
    26041 ggcagcgcta gagcattttc tgaaaaatga tgagcgtggt caggaaatct tgtggagtct
    26101 ggaagaaggt catgaagaaa aagaggccgt tgaaaccaag gtgaccttgg gtggcaagtt
    26161 tttgaatcga attttatctc caaaatcaga atgcgcctat gagttatcag ctgtgggtca
    26221 ggtggaagca ggaaccaatc atatcttgtg gactctgaga atcggccaaa tcaatagcca
    26281 aaaatactat gtcattcggg atattccact ttttttaagg attgtcgagc agagaaagtc
    26341 gtatatgatt ggcaagattt atgaggagtc tctttcttgg gaagcctttg atgatgccag
    26401 ccaagaactt ttgactttct tgcacggact aacggaggaa gggctggttc cagacctctt
    26461 tttccaaaac caaggtcggc acctcttttt ccctctgacc ttttttgagc agggtgtgaa
    26521 tttactgatg gccttgcccc attttcaatt tgaccatcaa gtggatagct accaaaccct
    26581 gctttttcag gatatgcatg ctgatgccaa tctatttgcc tttacagtaa cagaatactc
    26641 ggactatttt gaaatggaaa tcagtgagag tcctagggtc aatgtctttt atcagggagc
    26701 agtacttttc cataaaggtc aggtttattt tttaacagac cagcagatgc gtcttctcaa
    26761 ggaaattaaa gcgttacctg tgggtcaaca aggaaagaaa tacctgcaat ttgatagcag
    26821 tgatcgagac aaattggcct cctgtctgac tctttttatc cagatgggca ccgtttcagc
    26881 tccagaacgt ctacaaatca aaacttttgc gccctctttt tactttgaca gggaagaaga
    26941 taatcggatt cgtttagaaa ttcagtttga ttatggaaat agacaggtat ctagccgaca
    27001 agagctagaa gaattaccat tttcaagtga cgcggactta gaagaaagaa ttttccaagt
    27061 ctgtttggct gcgggctttg aagccgattt tcaatcttgg cgtcaggcct taaaagcgga
    27121 gtctgtctat catttttttc atgatatcat tccagtcttt gaaaaactcg ggcatgttga
    27181 cctatcagat aaactagaag agctttatag cctagcgagt cctcaggtgc agattgcctc
    27241 caagggaggt ctcttggaaa tccagtttga ttttcaaaat attgcccagg aggaaatcga
    27301 ccaagccatg caggccttgg ttgccaatca ggatttttat attgattcgt ctaatcaagt
    27361 ttactttttc gatgaagaaa ccaagaaaat tcgccaaaat ctacaggaac tggggcaatt
    27421 tgaattaaaa gatgggacct tgcaggctcg gaaatccttg gcctacagtt tagctcatct
    27481 ctttgaaggg cgagaccgtg tttctttttc acaagaattc cagaatctgg cccaggattt
    27541 gacgcatcca gaggactttc ctttgcaagc aactcaggtc aaggctgact tgcgagatta
    27601 tcaggaaaag ggaattggct ggttgcagat gctccatcat tatggttttg gtgggatttt
    27661 ggctgatgat atgggacttg ggaaaaccct tcagaccatt gcttttttga ctagtcaagt
    27721 gacaaaagaa agtcgagtct tgattctcgc tccgtcgggt ttgatttaca actgggcgga
    27781 tgagtttcag aaatttgccc cacagttgga tgtggctgtt gttcatggtt tgaaagcaag
    27841 tcgtgaggag attcttgccg agagccatca aatctatgtg actagctatg ccaccttccg
    27901 tcaggacagt gagttttatc aggggatggc ctttgacttc cttttcttag atgaggctca
    27961 ggtcatgaaa aatgcccaga ccaagattgc ccagaccttg agacaatttg tggtgccgtc
    28021 ggtctttgcc ttgtcaggaa ctccgattga aaaccatctg ggtgagttgt ggtctatttt
    28081 ccaaatcgtc atgcccggac ttttgccaag caagaaagaa tttatgaaat tgccagcaga
    28141 gcgagtggct cagtttatca agcctttcgt gatgcgacgc aagaaagaag aagttctgac
    28201 tgaattgcca gacttgattg aggtggttta taaaaatgaa ctggaagacc agcaaaaggc
    28261 tatttacctt gcccagttac aacagatgcg agaccatctg gctcaagtgt cagaacagga
    28321 atttcagcga agtcgtgtgg aaattttatc tggtttgatg cgcttgcgtc aaatatgtga
    28381 cactcctgcc ctgtttatgg aagattatca gggagccagc ggcaaactag atagtctccg
    28441 agacctactg gtacaggtgg cagacggcgg acaccgtgtc ttgattttct ctcagttcaa
    28501 gggaatgttg gaaaaaattg aacaagaact gccagacttg ggcctgactt cctttaaaat
    28561 tacgggttca accccagcca aggaaagaca agacatgacc aaggccttta atcaaggaga
    28621 aagagatgcc tttctgattt ccctcaaggc tggtggggtc ggtctgaacc tgacaggtgc
    28681 agatacagtg atcttggttg acctttggtg gaatcctgcg gtggaagcgc aagccattgg
    28741 ccgtgcccat cggatgggtc aggaagaaac ggttgaggtc tatcgcttgg tgaccaaggg
    28801 aaccattgaa gaaaaaattc aggaactcca agaacaaaag aaacatctgg tgtcacaagt
    28861 attggacggc acagagtcac gtggtagtct gaccctagca gaaattagag aaattttggg
    28921 aatttctgaa gccagcactt gaaaaatcaa gacaatacgc tataatgaag tgttgaaagg
    28981 aaatagaaaa tgaaacaaga acgatttcca ttggtgtcag atgacgaggt catgttgact
    29041 gaaatgccag tcatgaatct ctatgatgag tctgatctga tcagtaatat caagggtgag
    29101 tatcgagata aaaattattt agaatgggct cctattgctg aagaaaaacc agtaaaaccg
    29161 attgaaaagc aagtagggaa acctaaaaaa gctcctttag gggttaaaaa agaaggaaag
    29221 agctatgcgg aggtggcgcg tgaagaagcg cgtgcagact tgaaaaagaa acgctctgct
    29281 aactatctaa ctcaggattt cagccttgcg agacgtcatt ctcagcccag tctagttaga
    29341 cagggcaatc aaccgacaac tcctttccaa aaggaaaatc ctggtgaatt tgtcaaatat
    29401 agccaaaaat tgacccagtc tcattatatc ttggcggaag aagttcattc tatccctacc
    29461 aagaatgaag aagtgtcagc acctgctcca aagaaaaaca attatgattt tctaaagaag
    29521 agccaaatct acaataaaaa aagtaaacaa acagaacaag aacgtcgggt tgcccaagag
    29581 ttgaatctga ccagaatgac agaataggga gaaaacatgt caaagacata tcattttatc
    29641 ggaattaagg gatcagggat gagtgccttg gccttgatgt tgcaccagat ggggcacaag
    29701 gttcagggat cagatgttga aaagtactac tttacccaac gcggtcttga gcaggcagga
    29761 attaccattc ttccttttga tgaaaagaat ctagacggtg atatggaaat tatcgctgga
    29821 aatgcctttc gtccagataa caacgtcgaa attgcctatg cggaccaaaa tggtatcagc
    29881 tacaaacgtt accatgagtt tctaggtagc tttatgcgtg actttgttag catgggagta
    29941 gcaggagcac atggaaaaac ttcaacgaca ggtatgttgt ctcatgtctt gtctcacatt
    30001 acagatacca gcttcttgat tggagatggg acaggtcgtg gttcggccaa tgccaaatat
    30061 tttgtctttg aatctgacga atatgagcgt cacttcatgc cttaccaccc agaatactct
    30121 attatcacca acattgactt tgaccatcca gattatttca caagtctcga ggatgttttc
    30181 aatgccttta acgactatgc caaacaaatc accaagggtc tttttgtcta tggtgaagat
    30241 gctgaattgc gtaagattac gtctgatgca ccaatttatt attatggttt tgaagctgaa
    30301 ggcaatgact ttgtagctag tgatcttctt cgttcaacaa ctggttcaac cttcaccgtt
    30361 catttccgtg gacaaaactt ggggcaattc cacattccaa cctttggtcg tcacaatatc
    30421 atgaatgcga cagccgttat tggtcttctt tacacagcag gatttgattt gaacttggtg
    30481 cgtgagcact tgaaaacatt ttccggtgtt aaacgtcgtt tcactgagaa aattgtcaat
    30541 gatacagtga ttatcgatga ctttgcccat catccaacag aaattattgc gaccttggat
    30601 gcggctcgtc agaaataccc aagcaaggaa attgtagcag tctttcaacc gcataccttt
    30661 acaagaacca ttgccttgtt ggacgacttt gcccatgctt taaaccaagc agatgctgtt
    30721 tatctagcgc aaatttatgg ctcggctcgt gaagtagatc atggtgacgt taaggtagaa
    30781 gacctagcca acaaaatcaa caaaaaacac caagtgatta ctgttgaaaa tgtttctccg
    30841 ctcctagacc atgacaatgc tgtttacgtc tttatgggag caggagacat ccaaacctat
    30901 gaatactcat ttgagcgtct cttgtctaac ttgacaagca atgttcaata ggatgttccc
    30961 atggaaattc caattaagat cattcaggca agcaagtctg atttgcctga gataggggca
    31021 cttcagacct cgtcttttcc agctgaaaag cagcaacttt cccatatttt agaagaaagt
    31081 atccgtaagt gtgcggatac ctttcttcta gctagggatg aaaatcaact tttaggctat
    31141 attttatcaa gtccccagtc agacaatccg caatatctaa aagtacattc tttagtcatc
    31201 gagtctgacc atcagagaca gggcttggga acacttcttc ttgcagcctt gaaagaggtg
    31261 gcagttgatc tggattacaa agggattcgt ttggagagtc ctgatgagct gctttcctat
    31321 tttgaaatga acggttttgt tgatgaagaa gcaactttgc tctatgcaac tagccagggc
    31381 tatagtatga tttggtttaa tcccttttat ctggaggaac aatgaaaatc agacaagcaa
    31441 gattagaaga tttggatcgg attgttgaac ttgaatttga aaatttttcg gtcgaagaag
    31501 ccattcctcc ttctgtcttt gaagcacatt tgagagaaat tcagacctct tttctggttg
    31561 ctgaaaaaga aggaagaatc atgggttata tcgaaggacc agttggcctg caccgccatc
    31621 tgcaagacca gtcttttaca gaagaaataa aagactatag tcatgagcct ggtggttata
    31681 tctctgtgac ctgtctgtct attgccaagg aggcacaggg actcggactg ggtcagaaat
    31741 tgctgacagc tttgaaagaa gttgctcttg aagatgagag agatggcatt aatctaacct
    31801 gtcatgacta tctcatcgcc tattatgaaa aacatggatt tgtcaacgaa ggccagtccc
    31861 agtcaacctt tgcaggggaa acatggtatg atatggtctg ggaaatgaaa aaataagtta
    31921 ggaaaagtat catagattgc tttttttgga cttttaagat ataatattat ggattatcaa
    31981 caaggaggaa aaacttttga gtgaaaagtc aagagaagaa gagaaattaa gctttaaaga
    32041 gcagattctg agagatttag aaaaagtaaa aggctatgat gaagttctga aagaagatga
    32101 ggcagtagtt cgcactcctg caaatgaacc ttcagctgaa gaactcatga ctgattcctt
    32161 gtcaacggta gaggagatta tgagaaaagc tcctaccgtg cctactcacc caagtcaagg
    32221 tgtaccagct tctccagcag atgagattca aagagaaact cctggtgttc caagtcatcc
    32281 aagtcaagat gtaccttctt ctccagcgga agaaagtgga tcaagaccag gtcctgttag
    32341 acctaagaaa cttgaaagag aatacaatga aaccccaaca agggtagctg tttcctatac
    32401 gacggcagag aaaaaagcag aacaagcagg tccagaaaca cctacgcctg ctacagaaac
    32461 agtggatatc atcagcgata catcacgtcg tagccgtaga gaaggagcaa aacccgttaa
    32521 tcctaagaaa gagaagaagt cacatgtgaa agcttttgtg atttcattcc ttgtattcct
    32581 tgccttgctc tcagcaggtg gttactttgg ttaccagtac gtgctagatt ctttgttacc
    32641 tatcgatgct aattctaaga aatatgtgac ggttggaatt ccagaaggtt caaacgttca
    32701 agaaatcggt acgacgcttg aaaaagctgg tttggtaaag catggtctga tttttagttt
    32761 ttatgccaag tataaaaatt ataccgactt gaaagcaggt tactacaatt tgcaaaagag
    32821 tatgagtaca gaagacttac tcaaagagtt gcaaaaaggt ggaacagatg aaccgcaaga
    32881 acctgtactt gcgactttga caattccaga aggttatacc ttggatcaga ttgctcaagc
    32941 tgtgggtcaa ttgcaaggtg acttcaaaga gtctttgaca gcggaggctt tcttggctaa
    33001 agttcaagat gagacgttta tcagtcaagc agtagcgaaa tatcctactt tactggaaag
    33061 tttgcctgta aaagacagcg gtgcgcgtta tcgtttggaa ggataccttt tcccagctac
    33121 atactctatc aaggaaagca caactattga gagcttgatt gatgagatgt tagctgctat
    33181 ggataagaac ctatctcctt actatagtac tatcaaatct aaaaatttga ctgtcaatga
    33241 gttgttgacc attgcttcct tggtcgaaaa agaaggtgcc aagacagaag atcgtaagct
    33301 cattgcaggt gtattctaca atcgtttaaa tcgtgatatg ccacttcaaa gtaatattgc
    33361 aatcttgtat gcccaaggaa aactggggca aaatatcagt ctagctgagg atgttgcgat
    33421 tgataccaac attgattcac cttataatgt ttataaaaat gtaggtctca tgcctggtcc
    33481 agtcgatagt ccaagtctgg atgcgattga gtcaagcatc aatcaaacta agagcgataa
    33541 cctctacttt gtagcagatg tcacagaagg caaggtctac tatgctaaca atcaagaaga
    33601 ccacgaccgc aatgtcgctg aacatgtcaa cagcaaatta aactaaacaa actaaaatta
    33661 tgtgatacgt cacataattt tctttagaaa atattatcag aagaaagttg agaaaaatgg
    33721 cagaaaaaac atatcctatg acccttgagg aaaaggaaaa acttgaaaaa gaattagaag
    33781 aattgaaatt ggttcgtcga ccagaagtgg tagaacgcat taagattgcc cgttcatacg
    33841 gtgacctttc agaaaacagt gagtacgaag cagctaagga tgaacaagcc tttgtcgaag
    33901 gacaaatctc tagcttagaa acaaaaatcc gctatgctga aatcgtcaat agcgacgcag
    33961 ttgcccagga cgaagtagcg attggtaaaa cagtcaccat ccaagaaatt ggtgaggacg
    34021 aagaagaagt ttatattatc gtaggttcag ctggtgcgga tgcctttgca ggtaaggttt
    34081 caaatgaaag cccaattggg caggccttga ttggcaagaa aacaggtgac acagcaacca
    34141 ttgaaacgcc tgttggtagc tatgatgtaa aaatcttgaa ggttgaaaaa acagcctaaa
    34201 aacagaaaaa ggagtgggga ggcgatgtgc ttcactcact cctttttcca ttttgctatt
    34261 cttcgaaaat ctcttcaaac cacgtcagcg tcgccttgcc gtatgtatgg ttactgactt
    34321 tgtcagtttc atctacaacc tcaaaac
//
LOCUS       DAVBBS010000017        33324 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVBBS010000017 DAVBBS010000000
VERSION     DAVBBS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33324
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.017"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..327
                     /locus_tag="VQU32_000807"
     CDS             <1..327
                     /locus_tag="VQU32_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2472286.1"
                     /translation="KPQTEKPEEETPREEKPQSEKPESPKPTEEPEEESPEESEEPQV
                     ETEKVEEKMREAEDLLGKIQDPIIKSNAKETLTGLKNNLLFGTQDNNTIMAEAEKLLA
                     LLKESK"
     gene            749..979
                     /locus_tag="VQU32_000808"
     CDS             749..979
                     /locus_tag="VQU32_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HET2472287.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAIKC"
     gene            1383..2096
                     /locus_tag="VQU32_000809"
     CDS             1383..2096
                     /locus_tag="VQU32_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HET2472288.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            2154..2756
                     /locus_tag="VQU32_000810"
     CDS             2154..2756
                     /locus_tag="VQU32_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472289.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCMGDFPHDFF
                     DIYLDHVAKYAYEQKVNNIKEYYPLKRAILHQENALYFRFFSNFDDFLEKNYLKTIWQ
                     VSKETPFSEMDFNMFKNISEKIIFERGSKMLNDLKSNYKK"
     gene            2892..3545
                     /locus_tag="VQU32_000811"
     CDS             2892..3545
                     /locus_tag="VQU32_000811"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET2472290.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            3555..4019
                     /locus_tag="VQU32_000812"
     CDS             3555..4019
                     /locus_tag="VQU32_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET2472291.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            4032..5300
                     /locus_tag="VQU32_000813"
     CDS             4032..5300
                     /locus_tag="VQU32_000813"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET2472292.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            5314..5574
                     /locus_tag="VQU32_000814"
     CDS             5314..5574
                     /locus_tag="VQU32_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472293.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            5585..6946
                     /gene="pdrM"
                     /locus_tag="VQU32_000815"
     CDS             5585..6946
                     /gene="pdrM"
                     /locus_tag="VQU32_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HET2472294.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            7100..8068
                     /locus_tag="VQU32_000816"
     CDS             7100..8068
                     /locus_tag="VQU32_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET2472295.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            8084..9076
                     /locus_tag="VQU32_000817"
     CDS             8084..9076
                     /locus_tag="VQU32_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET2472296.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDKNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYISEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            9294..10337
                     /locus_tag="VQU32_000818"
     CDS             9294..10337
                     /locus_tag="VQU32_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HET2472297.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            10401..12086
                     /gene="lpdA"
                     /locus_tag="VQU32_000819"
     CDS             10401..12086
                     /gene="lpdA"
                     /locus_tag="VQU32_000819"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET2472298.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEIIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMQSPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEIIGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            12150..13139
                     /locus_tag="VQU32_000820"
     CDS             12150..13139
                     /locus_tag="VQU32_000820"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HET2472299.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(13206..14276)
                     /gene="xerS"
                     /locus_tag="VQU32_000821"
     CDS             complement(13206..14276)
                     /gene="xerS"
                     /locus_tag="VQU32_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HET2472300.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
     gene            complement(14860..16410)
                     /locus_tag="VQU32_000822"
     CDS             complement(14860..16410)
                     /locus_tag="VQU32_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET2472301.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFIIIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGSLVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(16426..17205)
                     /locus_tag="VQU32_000823"
     CDS             complement(16426..17205)
                     /locus_tag="VQU32_000823"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET2472302.1"
                     /translation="MATIKEIKELLVTVKELESPIFLDLEKDNRSGVQKEISKHKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQVVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(17192..18043)
                     /gene="ylqF"
                     /locus_tag="VQU32_000824"
     CDS             complement(17192..18043)
                     /gene="ylqF"
                     /locus_tag="VQU32_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HET2472303.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EGLDGND"
     gene            complement(18250..18426)
                     /locus_tag="VQU32_000825"
     CDS             complement(18250..18426)
                     /locus_tag="VQU32_000825"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2472304.1"
                     /translation="MQAGIPTDNEVSYALIYQQETLPATGSSTSALTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
     gene            complement(18478..24369)
                     /gene="iga"
                     /locus_tag="VQU32_000826"
     CDS             complement(18478..24369)
                     /gene="iga"
                     /locus_tag="VQU32_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin A1 protease"
                     /protein_id="HET2472305.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGSIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVTDKG
                     ETEVQPESPDTVVSDKGEPEQVAPLPEYKGNIEQVKPETPVEKTKEQGPEKTEEVPVK
                     PTEETPVNPNEGTTEGTSIQEAENPVQPAEESTTNSEKVSPDTSSENTGEVSSNPSDS
                     TTSVGESNKPEHNDSKNENSEKTVEEVPVNPNEGTVEGTSNQETEKPVQPAEETQTNS
                     GKIANENTGEVSNKPSDSKPPVEESNQPEKNGTATKPENSGNTTSENGQTEPEKKLEL
                     RNVSDIELYSQTNGTYRQHVSLDGIPENTDTYFVKVKSSAFKDVYIPVASITEEKRNG
                     QSVYKITAKAEKLQQELENKYVDNFTFYLDKKAKEENTNFTSFSNLVKAINQNPSGTY
                     HLAASLNANEVELGPDERSYIKDTFTGRLIGEKDGKNYAIYNLKKPLFENLSGATVEK
                     LSLKNVAISGKNDIGSLANEATNGTKIKQVHVDGVLAGERGVGGLLAKADQSSIAESS
                     FKGRIVNTYETTDAYNIGGLVGHLTGKNASIAKSKATVTISSNTNRSDQTVGGLAGLV
                     DQDAHIQNSYAEGDINNVKHFGKVAGVAGYLWDRTSGEEKHAGELTNVLSDVNVTNGN
                     AITGYHYTGMKVANTFSSKANRVFNVTLEKDEVVSKESFEERGTMLDASQIVSKKAEI
                     NPLTLPTVEPLSTSGKKDSDFSKIAHYQANRALVYKNIEKLLPFYNKSTIVKYGNLVK
                     ENSLLYQKELLSAVMMKDDQVITDIVSNKQTANKLLLHYNDHSSEKFDLKYQTDFANL
                     AEYNLGNTGLLYTPNQFLYDRDSIVKEVLPELQKLDYQSDAIRKTLGISPEVKLTELY
                     LEDQFSKTKQNLGDSLKKLLSADAGLASDNSVTRGYLVDKIKNNKEALLLGLTYLERW
                     YNFNYGQVNVKDLVMYHPDFFGKGNTSPLDTLIELGKSGFNNLLAKNNVDTYGISLAS
                     QHGTTDLFSTLEHYRKVFLPNTSNNDWFKSETKAYIVEEKSTIEEVKTKQGLAGTKYS
                     IGVYDRITSATWKYRNMVLPLLTLPERSVFVISTMSSLGFGAYDRYRSSDHKAGKALN
                     DFVEENARETAKRQRDHYDYWYRILDEQSREKLYRTILLYDAYKFGDDTTSGKATAEA
                     KFDSSNPAMKNFFGPVGNKVVHNQHGAYATGDGVYYMSYRMLDKDGAITYTHEMTHDS
                     DQDIYLGGYGRRNGLGPEFFAKGLLQAPDQPSDATITINSILKHSKSDSTEGSRLQVL
                     DPTERFQNAADLQNYVHNMFDLIYMMEYLEGQSIVNKLSVYQKMAALRKIENKYVKDP
                     ADGNEVYATNVVKELTEAEARNLNSFESLIDHNILSAREYQSGDYERNGYYTIKLFAP
                     IYSALSSEKGTPGDLMGRRIAYELLAAKGFKDGMVPYISNQYEEDAKQQGQTINLYGK
                     ERGLVTDELVLKKVFDGKYKTWAEFKTAMYQERVDQFGNLKQVTFKDPTKPWPSYGTK
                     TINNVDELQALMDQAVLKDAEGPRWSNYDPEIDSAVHKLKRAIFKAYLDQTNDFRSSI
                     FENKK"
     gene            complement(24578..25756)
                     /locus_tag="VQU32_000827"
     CDS             complement(24578..25756)
                     /locus_tag="VQU32_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472306.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCRNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLPLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWILNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            complement(25926..29576)
                     /gene="addA"
                     /locus_tag="VQU32_000828"
     CDS             complement(25926..29576)
                     /gene="addA"
                     /locus_tag="VQU32_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HET2472307.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEICSLNQESDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FAKLGQLSDQITILDYQERYHQDTWKLAKTFQTFMSHFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALVEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQNFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIDEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQIIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTALGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            complement(29573..32848)
                     /gene="rexB"
                     /locus_tag="VQU32_000829"
     CDS             complement(29573..32848)
                     /gene="rexB"
                     /locus_tag="VQU32_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HET2472308.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQTTEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPISPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            complement(33048..33203)
                     /locus_tag="VQU32_000830"
                     /pseudo
     CDS             complement(33048..33203)
                     /locus_tag="VQU32_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
BASE COUNT         9515 a         6538 c         6861 g        10410 t
ORIGIN      
        1 aaacctcaga cagaaaaacc tgaggaagaa acccctcgag aagagaaacc gcaaagcgag
       61 aaaccagagt ctccaaaacc aacagaggaa ccagaagaag aatcaccaga ggaatcagaa
      121 gaacctcagg tcgagactga aaaggttgaa gaaaaaatga gagaggctga agatttactt
      181 ggaaaaatcc aggatccaat tatcaagtcc aatgccaaag agactctcac aggattaaaa
      241 aataatttac tatttggcac ccaggacaac aatactatta tggcagaagc tgaaaaacta
      301 ttggctttat taaaggagag taagtaaagg tagcagcatt ttctaactcc taaaaacagg
      361 ataggagaac gggaaaacga aaaatgagag cagaatgtga gttctagttc tcattttttt
      421 catgaaaatg tgcaaaatat agtagattga aactagaata gtatacctct acttctaaaa
      481 cattgttaga aatcgatttg actgtcctgt tcttatttca ttttactata tcttaacaga
      541 tagtgtaaat aaagataaac tatttactgg ctaattaatc agttaaacac tagttaagga
      601 gtaatgatga aaaaaagaac aatactatta ttgatggcca gtctgttagc tcttgtctta
      661 ggagcatgtg gtttcttgga catattgatc ctggatcatt ctcatcagga ttactcttta
      721 ctgctatttt agaaactggg gtggtttgat ggaaagtatt ggtattggtc ttgttatcgt
      781 ttcacattcc aaacacattg cagaaggtgt tgttgaactg attagtaaag tagctaaaga
      841 tgttccgatt acttatgtag gaggaaccga gggcggagga attggaacga gttttgatca
      901 agtagatagg gttgtttccg aaaatccagc agatacttta cttgcctttt ttgacctagg
      961 ttctgctata aaatgttaag taaatgattt caaaatcaga aagggattgc tttgggcagt
     1021 tcctttttaa tgtaaatgta aaaggagtag aatcatagtg tttctaaatt gtgaatcaat
     1081 caaaactgat tgtgatgggg ctattctagc tttagaatcc ttcaaaaatt aaaatttaag
     1141 gcaatcaatt acttggaaga gtatgaaagc atttagttta taggaattct aggcctagaa
     1201 ttacatatgt atttatgaag ataggggtgt tcgatagtta gtattgttct gttctgaaag
     1261 atttgagctg tcagttgtat agaaagtgtt cgaatttttt ttaagtgatt aaattagtta
     1321 attgtatgag gttctttatg atataatgtt cttaatgaat tttcagaaag gaaaacctca
     1381 aattgttcta caaatttcta ctcttcgacc tcgaccacac tcttcttgat tttgatgctg
     1441 ctgaggatgt ggctttgacc caacttctaa aagaagaagg agttgcggat attcaggctt
     1501 ataaagatta ttacgttcct atgaacaagg ctctctggaa agacttggag ctgaagaaaa
     1561 tcagtaaaca agagctggtt aacacgcgct tttctcgttt atttgctcat tttggacagg
     1621 aaaaagacgg tagttttctt gcccagcgtt accaatttta cctcgcccag cagggacaaa
     1681 cactatcggg cgctcatgat ctcttggaca gcctcattga gcgtgattat aacttgtatg
     1741 ctgcgacaaa tggcattact gccattcaga caggacgttt ggctcaatct ggtctagcac
     1801 cttatttcaa tcaagtcttt atctcagaac agttgcaaac tcaaaagccg gatgctctct
     1861 tttatgaaaa gattggccag caaattgctg gatttagtaa agaaaagacg ctgatgattg
     1921 gagattctct aaccgccgac attcaaggtg gcaataatgc ggggattgat actatctggt
     1981 ataatcctca tcacctcgaa aatcacacac aagcccagcc gacttacgaa gtctattctt
     2041 accaagactt gctggattgt ttagataaaa atattcttga aaagatcaca ttttaaagga
     2101 gacgagctaa tgactacaaa aaagctaata ttactattga agagtacatt gaaatgtctg
     2161 aagttgattt taatgaagct gttaattatg aatttacatc tgacacttgt caattagcaa
     2221 atagtattta tcaatctctt tttaagtttt ttgataagaa aaatttctct ggcgatttaa
     2281 tttttacttg gaaatctcca tcattagtca aagaagggga ttatattggg agaagggatt
     2341 cacaagtaga taatcttaga gtaataggaa atatatttcc gaattatctt actaatcgaa
     2401 aatatagcct caatatgaat cgtaatggct gtatgggaga ttttcctcat gacttttttg
     2461 atatatacct agatcatgta gcaaaatatg cctacgaaca aaaagttaat aatattaaag
     2521 agtattatcc tttaaaaaga gcgattttac accaagagaa tgcattgtat tttcgatttt
     2581 tttctaattt tgacgacttt ttagaaaaaa attatttaaa gactatatgg caagtttcta
     2641 aagaaactcc tttttctgaa atggatttta atatgtttaa aaatatttca gaaaagataa
     2701 tcttcgaaag aggtagtaaa atgcttaatg atttaaaaag taattataaa aaatagccag
     2761 tctttataaa ctgtattcac gtattcaaat cgtatatagt cgatttatta aatctttaga
     2821 taaactgtaa aaagtggttt ccatagccac tttttgctat aatagaggca gtaaaaatga
     2881 aggagtcggc tatggaacac tcgtcttggc atgctttgat taaggcgcaa ttacctgagg
     2941 gttatttcgg gaaaatcaat cagtttatgg agcaggtcta ttctcagggg attatttatc
     3001 cacccaagga aaaggttttt caggctctct tgacaacact gcttgaagaa gttaaggtgg
     3061 taattctagg gcaagacccc tatcacggac caggtcaagc gcagggcttg agtttttctg
     3121 tacctgactc tatcccagct ccaccatcct tgcaaaatat cttgaaagaa ttgtcagatg
     3181 atatcggagt taagaaatct catgatttga cagcttgggc tgagcaagga gtcttgcttc
     3241 ttaatgcttg tttgactgtt cctgctggac aggccaatgg tcatgctggt caaatctggg
     3301 agccttttac tgatgctgtg attcaggtgg tcaatcatct agatagacca gtcgtttttg
     3361 tactctgggg agcttatgca cgtaagaaga aggccttagt taccaatcct catcacttga
     3421 ttatcgaatc agcccatcca agtcctttgt cggtttatag aggattttgg ggttccaagc
     3481 ccttttccaa ggccaatgca ttcttaaaag agacaggaca agagccaatc gattggctta
     3541 gataaggaga aaatatgcct cagttagcga cgatttgcta cattgataat gggaaagaac
     3601 tgctcatgct ccatcgtaat aagaaaccca atgatgtcca tgaagggaaa tggattggtg
     3661 tgggtggtaa gctagagaga ggagagacgc cccaggaatg cgcggcgcgt gaaatccttg
     3721 aagaaacagg gctcaaagcc aagccagttc taaaaggtgt catcactttt cctgaattta
     3781 caccagattt agactggtac acctatgttt ttaaggtgac ggagtttgag ggcgacttga
     3841 ttgactgcaa tgaggggacg ctagaatggg ttccctatga cgaggttttg agcaagccga
     3901 cttgggaagg tgaccacacc tttgttgagt ggcttttaga ggataaaccc ttcttttcag
     3961 ccaagtttgt ttatgatggg gataaattgt tggataccca agttgatttc tatgaataaa
     4021 ggagaaagca gatgctacta atcaaaaatg gtcgtgtaat ggatcctaag tctggtttgg
     4081 atcaagtttg tgatgtctta gttcaagatg ggaaaattat caaaattgcg cctgagatca
     4141 cggaagaagg agcagaaacg attgatgcta ctggtcttgt ggttgctcct ggtttggtcg
     4201 atattcatgt gcatttccgt gaacctggtc aaacacataa agaagacatt catactggtg
     4261 ccctggcagc cgctgcaggt ggttttacta ctgtcgtcat gatggctaat accagtccaa
     4321 ccatttcaga cgtggagact ttgcaagcag ttctccagtc agctgccaaa gagaagatta
     4381 atgtcaagac agttgcgacc attactaaaa actttaatgg taaaaacttg actgacttta
     4441 aggcgctctt agaagctggt gcggttggtt tctctgatga cggtattccg cttgagagca
     4501 gtaagattgt caaggaagcc atggaggaag ccaaaaaact caataccttt atcagccttc
     4561 atgaggaaga tccaggtttg aacggtgttc ttggctttaa tgaaaatatt gctagagaac
     4621 atttccatat ctgcggtgct actggggtgg ctgagtacgc tatgatggcg cgtgatgtca
     4681 tgattgccta tgcaactaaa gcccatgttc acatccagca tttgtctaag gaagaaagtg
     4741 ttaaagtagt ggagtttgct caggggttag gtgcagaagt cacagcagaa gtagcgccac
     4801 agcatttctc taagacagaa gcacttcttt taacacaagg tagcaatgct aagatgaatc
     4861 caccgcttcg tttggaatca gaccgtcgtg ccgttatcga aggtctcaaa tcaggtgtca
     4921 tcacagttat tgcgactgac cacgcgcctc atcatgtaga tgaaaaaaat gttgaggata
     4981 ttaccaaagc gccatctggt atgactggct tagaaacatc cctgtctctc ggcttgacct
     5041 atttagtaga agctggtgag ttgagcttga tggaattact tgaaaaaatg acatacaacc
     5101 cagccaagct ttacaacttt gaagcaggtt acttggctga gaatggtcca gcagatatca
     5161 ctatttttga tgccaaggct gaccgccttg tggactccca ttttgcttcc aaagcagcta
     5221 attcaccatt catcggtgaa accttaaaag ggcaggttaa atataccatc tgtaagggac
     5281 aaatcgtcta tcaagcttga taggagttag aatatgaatt attttcgaaa acgtagggag
     5341 agacaagcga aatctaatag tggaatttac tgtcccgcag ctaatcgttc caaaattcaa
     5401 tatgaaacta aagaaaaagc tgaccatgcg gttcaatatg ataacggagg tttagttaga
     5461 agctattatt gtcgtacatg tgcgtgttgg catactactt caaaatctaa taaacctccg
     5521 ctaagtttga aaaactatgg tctgaaactc tttggtttag ataaatcaga atagaaagag
     5581 aagtatgtat aagacaaagt gtttacgaga gaagttagta ttatttttaa aaattttctt
     5641 cccaatcctg atctaccaat ttgccaatta ttctgcctct tttgttgata ctgcaatgac
     5701 aggtcaatac aacactatgg acttggctgg tgtatctatg gcaaccagta tctggaatcc
     5761 tttctttaca tttctaacag ggattgtgtc agccttggtg cctatcattg gtcaccatct
     5821 tggtcgaggc aaaaaggaag aagttgcgtc tgatttttac caatttattt atttggcctt
     5881 gggcctatct gtggtcttgc tggggatggt acttttcttg gcaccaacaa tcttgaatca
     5941 tattgggtta gaagcagcag tagcggcagt agcggttcgc tatctttggt ttttatctat
     6001 cgggattatc cccttgttgc tctttagcgt cattcgttcc ttgctggatt cgctgggctt
     6061 gaccaaactg tccatgtacc tcatgctttt gttactccct ctcaatagcg gatttaacta
     6121 tctcttgatt tacggtgcct ttggtgttcc agaactggga ggggctggtg ctggtttagg
     6181 aacatccttg gcctactggg tcttgcttgg gatttctgtt ctggttttat ttaaacagga
     6241 gaagctcaaa gccttacacc ttgagaaacg aattccactt aatatggata aaattaagga
     6301 aggagttcgt ttaggtctgc ctattggggg aactgtcttc gcggaagtgg ctatcttttc
     6361 agtggttggc ttgattatgg ctaagttttc gtccttgatt atagctagtc accagtcagc
     6421 tatgaacttt tcaagtctta tgtacgcctt tcctatgagt atctcatcgg ctatggctat
     6481 tgtcgtttcc tatgaagtgg gagccaagcg atttgatgat gcgaaaacct atattggtct
     6541 aggaagatgg actgccctca tttttgcggc cttcacctta accttccttt acatttttag
     6601 gggaaatgtg gccagtcttt atggtaacga cccaaaattt atcgatttga cagcgcgttt
     6661 tttaacttat agtcttttct tccagttagc agataccttt gcggcgccgc ttcagggaat
     6721 tttgcggggg tataaggata cagttattcc tttttacctt ggtttgcttg gttattgggg
     6781 cgtagcaatc cctgtggcta cgctatttga ttccctaaca gattttggag cttattctta
     6841 ctggatcggc ttgattatta gtttgattgt gagtggggcg ctctaccgtt ggcgtttaac
     6901 tgtgattatg aagagatttg aatctttagc aaaatccaaa cgctaaaaag tttgtgaaaa
     6961 aatattcttg ataagaaaat cccttgtttt cacggggttt tcttttttat tttgtgaaat
     7021 ttcttttaga aaaactttga cagtatttgc caaaaacggt aaaatagtaa ggtaagaaga
     7081 aagttagaaa ggcaagaaga tgtcaacttt agataaaaat cttttgctag aaatgttccg
     7141 taagatggaa gaaatccgtc gcatggactt aaaaattgca caattagtaa agaaagggaa
     7201 agtgccagga atgacgcact tttctgttgg tgaagaggca gctaacgtgg gggctatgtt
     7261 agctctcaat ccagatgatc tgattacctc aaaccaccgt ggacacgggc aagctattgc
     7321 taaagggatc gacctcaacg gaatgatggc tgaaatcctt ggtaaataca ctggaacctg
     7381 taaagggaaa ggtggatcta tgcatatcgc tgaccttgat gctgggaacc ttggtgccaa
     7441 tggtatcgta ggtggtggta tggggatcgc tgtcggtgca gccctcagtc agcaaatgca
     7501 aaataccggt aaaatcgttg tctgcttctt tggagatggt gcgaccaatg aaggtgtttt
     7561 ccacgaagca gtgaacatgg cttctatctg gaacctgcca gtcattttct attgcattaa
     7621 caacggttac ggtatctctg cggatatcaa gaaaatgacc aatatagaac atatccatca
     7681 acgtagcgcc gcttatggaa ttcctggaat gttcatcgaa gacggtaaca atgtcatcga
     7741 tgtctatgaa ggatttcaga aagctgtaga ccatgttcgc agtggcaatg gtccagtctt
     7801 gattgaaagt gtaacttatc gctggctcgg tcactcatca tctgaccctg gtaaatatcg
     7861 tacgcgtgaa gaagtggaat tgtggaaaca aaaagatcca atcgaaaacc tccgcaatta
     7921 ccttattgaa aataacattg caagtgccga agaattggaa gaaatccaag cgcaagtaaa
     7981 ggaagcagta gaagcttctg ttaaatttgc agaggaaagc ccattcccac cgcttgaatc
     8041 agcctttgaa gatatttacg cagactaagg agaaagagat aaaatggaaa caaaaacaat
     8101 gtccttccgt gacaccatta tccttgctat gtctgaggaa atgcgtcgcg atgaaaatgt
     8161 gttcttgatg ggagaagacg tcggtgtctt cggaggagac ttcggtactt ctgttggaat
     8221 gcttgaagaa tttggtccag aacgcgtccg tgactgtccg atttctgaag ctgccatctc
     8281 tggagcagca gcaggagcag ccatgacagg acttcgtcca atcgtcgata tgaccttcat
     8341 ggacttctct gttattgcca tggacaatat cgtcaaccaa gctgctaaaa cacgttacat
     8401 gtttggtggt aaaggtcagg ttccaatgac tgttcgttgt gcagctggta acggagttgg
     8461 ctctgccgcc cagcactcac aatctctaga gtcttggttt actcacattc caggacttaa
     8521 ggttgtggca cctggaacac ctgcggacat gaaaggcctg cttaagtctt ctatccgtga
     8581 taaaaaccca gttattatac ttgagtacaa gtcagaattt aaccaaaaag gggaagtgcc
     8641 agttgatcca gactacacaa ttccacttgg ggttggtgaa atcaaacgcc aaggaacgga
     8701 tgtaacagtt gttacttatg gaaaaatgct tcgccgtgtg gttcaagctg ctgaagaatt
     8761 agcagaagaa ggaatttcag ttgaaattgt tgacccacgt acccttgttc cgcttgataa
     8821 ggatatcatc ataaactcag tgaagaagac tggtaaggtt gttctggtca acgatgccca
     8881 caaaacaagt ggctatatca gtgaaatttc agctattatt tcagaatcag aagcatttga
     8941 ctatctagat gcaccaatcc gccgttgtgc aggagaagat gtgccgatgc cttatgcaca
     9001 aaacctagaa aatgcaatga ttccaacagt tgaaagtatc aaagatgcaa tccgaaaaac
     9061 ttataacaaa gaatagagtt acataagata agttatgtaa ataaaataaa gctgacgaga
     9121 aactttgtat cttttaggtg cagagctctt cgtcattaat atcttatatt cgagcacggg
     9181 ctaaaagctc ggaaaaaaga taaatctcct tggcttcatc gaagccatcg tcgatttcct
     9241 atttttcagt cgcttttgac gccctttgta tcataaatag aattggagag gtcatggctg
     9301 atgacaagct aagagcgact cctgcagcta gaaagttagc ggatgattta gggataaacc
     9361 tctacgacgt ttctggctca ggtgcaaacg gtcgtgtcca caaagaagac gtggaaactt
     9421 ataaagacac aaacgtggtt cgcatttcgc cacttgcaaa acgaattgcc ctcgaacata
     9481 acattgcttg gcaggaaatc caaggaaccg gtcatcgtgg taaaatcatg aagaaggatg
     9541 ttttggccct gcttcctgaa aatatcgaaa acgatagcat caaatctcct gctcagattg
     9601 aaaaagtgga agaagtccct gataacgtaa caccatatgg tgaaatcgag cgtattccaa
     9661 tgacaccaat gcgtaaggtt attgcccaac gcatggttga atcttacttg actgcgccaa
     9721 ccttcaccct caactatgaa gttgatatga ctgaaatgtt ggctcttcgt aagaaggttc
     9781 ttgagccaat catggaagca actgggaaga agactactgt aacagacctt ctttcgcttg
     9841 cagttgttaa gactcttatg aaacacccat acatcaacgc ttcattgaca gaagatggca
     9901 agactattat cactcacaac tatgtcaatc ttgccatggc agttgggatg gataatggat
     9961 tgatgacacc tgttgtttac aatgctgaga agatgagtct ttcagaacta gttgtagcct
    10021 ttaaggatgt tattggccgt accttggatg gtaaattggc tccaagtgag ttacaaaatt
    10081 caacattcac aatcagtaat ttgggaatgt ttggtgttca gtcctttggc ccgattatta
    10141 accaacccaa ctcagctatc cttggtgtca gttcgacaat cgagaagcca gttgtcgtca
    10201 atggtgaaat tgtgattcgc ccaatcatga gtttaggatt aaccattgac caccgtgtcg
    10261 tagatggtat ggctggtgct aagtttatga aggacttgaa agagttgatt gaaaccccaa
    10321 tctcaatgtt gatttaagag caagagtatt catttttaag atatagataa aggaaggaag
    10381 acatggcctt agaaataatt atgccaaaag ccggcgtgga tatgacagaa ggacaaatcg
    10441 tccaatggaa taaaaaagtc ggagaatttg taaaagaagg agaaatcctt ttggaaatca
    10501 tgactgataa agtcagcatg gaattggaag ccgaagaaga tgggtacttg attgccattc
    10561 tcaaaggaga tggtgaaact gtccctgtaa cggaaattat cggttacctt ggtgaagaaa
    10621 gggaaaacat cccaacagct ggagcagcat cgccagaagc tagccctgta cctgtagcaa
    10681 gtacctcaaa cgatgatggt aagagcgatg atgcttttga tatcgttgtg attggtggag
    10741 gtcctgctgg ttatgttgca gccattaaag ctgcccaact cggcggtaag gttgcccttg
    10801 ttgagaaatc tgaacttggt ggaacctgct taaaccgtgg atgtattcca accaagacct
    10861 accttcataa cgctgaaatt attgaaaata tcggtcatgc tgcaaatcgt ggtatcgtga
    10921 ttgaaaatcc taacttcact gttgatatgg aaaaactttt agaaactaaa tctaaagttg
    10981 ttaatactct tgttggtggt gttgcaggac ttcttcgtag ctacggagtt actgttcata
    11041 aaggaattgg tacaatcact aaagacaaga acgtcttggt aaatggttct gaattgcttg
    11101 aaaccaagaa aatcattctt gctggtggtt caaaagtcag caagatcaac gtccctggta
    11161 tgcaatctcc acttgtcatg actagtgatg acattcttga aatgaacgaa gtgccagaaa
    11221 gccttgttat catcggtggt ggagttgtcg gtatcgaact cggtcaggcc ttcatgacat
    11281 ttggttcaaa agtgactgtt atcgaaatga tggaccgtat agttccagct atggatgcgg
    11341 aagtttctaa gaaccttcgc ttgatccttg agcgtaaagg aatgaccatc ttgactggta
    11401 ctaaactgca agaaatcatt gaggaaaatg gtcaacttcg tatcaaggtt gaaggaaaag
    11461 acgatatcat cgcaagcaaa gctcttcttt caattggtcg tatgccagac cttgaaggta
    11521 ttggagaggt tgagtttgaa ttggatcgtg gttgtatcaa ggtcaacgaa tacatggaaa
    11581 cttcagttcc aggcatttat gcaccaggtg acatcaacgg tactaagatg ttggctcacg
    11641 cagctttccg catgggtgaa gtttccgctg aaaatgccct taaaggaaat catgcagttg
    11701 ccaaattgaa tttgactcct gcagccatct acactctccc tgaagtagca gcagtaggtt
    11761 tgacagaaga acaagcccgt gagaaatacg atgttgccat cggtaagttt aactttgctg
    11821 ctaacggtcg tgctattgca tctgacgcag ctcaaggttt cgtaaaagtt atcgctgata
    11881 agaaatacgg agaaatcatt ggtgtacaca ttattggtcc tgcagccgca gaattaataa
    11941 acgaagcatc aagcatcatc gaaatggaaa tcactgttga ggaaatgctg aagaccatcc
    12001 acggacaccc aacttactct gaagtgatgt atgaagcgtt tgcagatgtt ctaggaatgg
    12061 ccatccattc acctaagaaa aaataaaacg aagatagatt agactggaag gttatttcca
    12121 gtctctatca tataaagtaa agggatatca tgaaatacat tatcaatcat tcaaatgaca
    12181 ctgcttttaa tattgccttg gaagaatacg cctttaaaca ccttttggat gaggatcaaa
    12241 tcttcctact ttggattaac aagccatcta tcattgttgg tcgtcaccag aacactatcg
    12301 aagaaatcaa ccgtgattat gttcgcgaaa atggtattga ggtagttcgc cgtattagtg
    12361 gtggtggagc tgtttaccac gatttaaata acctcaacta cacgatcatc tccaaagaag
    12421 atgaaaataa ggcatttgac ttcaagagct tctcaactcc ggttatcaat accctggctc
    12481 aactcggggt taaagctgag ttcacaggtc gtaatgacct tgagattgat ggtaagaagt
    12541 tctgtggcaa tgcccaagcc tatatcaacg gccgtatcat gcaccacggt tgcttgctct
    12601 ttgacgttga tttgtcagtc ctcgcaaatg cccttaaggt ttcaaaagat aaatttgaat
    12661 caaaaggtgt gaaatccgtc cgtgcccgtg taaccaatat tatcaatgaa ttaccaaaaa
    12721 aaatcacagt cgaaaaattc cgtgatttac tcttggaata catgaaaaaa gagtacccag
    12781 agatgactga atacgtcttt tcagaagaag aattggccga aatcaatcac atcaaggata
    12841 ctaagtttgg aacttgggac tggaactatg gtaaatcacc tgaatttaac gtccgtcgtg
    12901 gaaccaaatt cactagtggt aaggttgaag tctttgctaa cgttactgaa tcaaaaatcc
    12961 aagacatcaa gatttatggt gacttctttg gtattgaaga cgttgctgca gtagaagatg
    13021 tccttcgtgg ggtgaaatac gaacgcgaag atgttcttaa ggcgctaaaa accattgata
    13081 tcacacgcta cttcgctggt attagccgtg aagaaatcgc tgaagcggta gttggataaa
    13141 tcaaaaaaag aagttggcca acttcttttt ttgatattta tttacataat ttaaaatacg
    13201 tgaaatcata aactatctag agcattcttt tgttcatcat taacaatatg ggtatagagg
    13261 tcagtgactt gtgtgctggc atgtcctagt tggtgactga ctaaaacttg tgatttagtc
    13321 gcatcataga gcctagttgc tagcgtatgg cgcagtttat ggggtgttac acgtacttta
    13381 aaatcctctg agtatttagc aaccattttc tcaacgctag aagcatcgat acgattagga
    13441 acacctctgt agagagttaa aaaaagggct gtatctgttt tttccgtttt atagcgttga
    13501 ttccgaatgg ccagataatt ctctaaataa ggtttagcaa aagcagcgac attgactgag
    13561 tcacgtttgc caccttttcg agtaacatca ataaccatca tttttagatt gagatctctt
    13621 agatctagat taacagcttc agataagcga acaccagatg ccaagagaag ggcaataatg
    13681 gctaaatctc gttctttatt tttgttgaat gatgagagag ctcgatttga aagctgttgt
    13741 gggtgctctt gatcgatata agttagaaaa ccttctgttt catcacctag aaagagtttt
    13801 tgcttgatat tttcagctct ggcagcaagg gtttctttct ttttcttggt tgaaactttt
    13861 ttcattacat tacgatagaa ataaggttcc ccctgatcgt tttcaacctc ctcggttaga
    13921 tacttgtaaa gactagaaag tgctgataag gttcgattga tagttgtctg tgaaacacct
    13981 tgttttgttg tattagcatt cagcaaggga cgttcacgta gataaaggat aaaggattcc
    14041 atgtctttct tagacatatt ttccaaaact gataaaggaa tatcggatat tttatcagcg
    14101 tttgaaatac cagactccaa aacccagctg aaaaatcggt catattcctt aaggtattcg
    14161 tacagggttg taaaactgta gggcacagcc agcttagatt ggtagtattc cagaacatac
    14221 cagggcatga gttgttttag tttgtcgatt cgttccagta aaatctcacg tttcatgtat
    14281 tttctccata agtaagtata ctagtagtat agcatagact tgtatatttt tcaagaaaat
    14341 tatagttttt cagaaagatg ttataggagt ttttcaaagt gataaaaaga cttagaccta
    14401 caaacaaaac agaataagga aaataataaa aatctcaccc actaaacttc tgttaatact
    14461 acataactga agagctatat aaggagctat gagatacaaa acacattgta taatagattg
    14521 aaactagaat agtacatctg gacttctaaa acattgctag aaattgattt gattgttctg
    14581 attgttttat cctatttcta tttcatttca ctatataatt gctattgttt tctttttcat
    14641 gattttaatc ttactgtaca gatttgatac agtaaactcc tttctagctt tatactttat
    14701 catttctaac ttaattatag tcttattttt aataaagttc aattatttat atgtaaaatt
    14761 cctcatcagt aaaaaataac cgatctcatt tctgagaatc ggttttctaa ataaatcaag
    14821 ctgagtattt acataacata aattatgtaa atactcatac tacaacaaca aatctttgac
    14881 ttttccaatt tcactttttg gaataaccag gtgaatcata tcacccagat acattctggt
    14941 tgagccgtta actgtttggc tcttgccatt atggacttga gttgtgatga ggacgttgtg
    15001 tggtaagttg agttcatgaa cttgtttccc agcaatttta tcagaaactg gtatttcgat
    15061 aagtgtaact tctccttcgc tagatacttc ttctggaagc attttttcca gcatggcttc
    15121 atagactggc gtacctttga gcaaatccat gataatataa gaaacaagag tgacaagacc
    15181 aagtggcatg aggttgcgaa tatctcctac catctcagtt acgaggatca tagcggttaa
    15241 gggagctttt gatatggctc caaaatagcc actcattcct agaatgacaa atatagggaa
    15301 ttgctcttga ctgacaagtc caagattgac acagataaca ccaactaagg aaccaagcaa
    15361 agaaccaaga gctaaaatgg ggaggaaaat tcctcctggc agtccacttc catagctaat
    15421 catactccaa ataaagcgga tcaaaaagta agctaataaa acttggaaac taaaattttg
    15481 ttcagttaaa gaaaggacaa gctgatttcc gccaccaatg atttgaggta agaagattcc
    15541 gactggtatg ataatgataa aagccaagat gggataataa gccctatcca aatggatttt
    15601 ttgaccaatc aagtcataaa ctcttccaac gtttaataca gctttctcat agagaaaacc
    15661 tgaaaatcca aggaaaattc ccatgacgag atatatccaa tactggtcta gggtcatggg
    15721 aggaatgtta tctggcatat ccaatactgg tgtcaaaccg aacatgagta gagacacaaa
    15781 gtttgctacg atgctggctg ctagagttga gacccagaaa aagcgcgaaa aatggtgata
    15841 gacttcttct acaacaaaga gaagtgctgc aataggagca ttaaaggctg cggctaaacc
    15901 tgctgcagct ccactggcaa tcaaggaacg ttcctctact ggactggatt tgagccactt
    15961 ggcaattcct ttaccaccaa ctgctccaag ttgaatgctg ggtccctctc gacccagcat
    16021 gagtccactg gcaatagcaa gaatacctag cacatatttt ttccaaagaa tgccccacca
    16081 gttgagggac atgaggcctt tcagttcggc ttcgacttga ggaattcctg agcctttaat
    16141 atctttttct gaccgtgtta gtttggcact gagccaacag atgagtatat aaaacaaaac
    16201 cagtacaaaa agattgcgca ctaagtaccc ttgatcttga taaactcctt gtatcaggtg
    16261 gaagcccttt tcaattaaga aacgaaagga tccgacgata attccaacga tgaggccgac
    16321 aatgattcct cgaccaactt gggatagtat agtgctggat gcaaaggcga attctttctt
    16381 agaacggact atttccgact gttcctccat ggtatttcct ttcaattaac tttctttttt
    16441 acctaaaacc agtgatttaa cgggttcaaa gctggttcgg tgaattgggg taactcctag
    16501 ttttgtgagg ccttccagat gtttagctgt gccatatcct gcattagtag cgaaatcata
    16561 gccagggaac tgctgatcgt attccttcat caattcatca cgtgttacct tggctactat
    16621 agatgctgct gcgatagaga gggagttggc atctcctttg ataatggagg tttgtgaaat
    16681 gggcaagtcc agtttcatgg catcaatcaa aaggtgctct ggttgagggc tgagctggga
    16741 gattgcttct tgcatggcta gtttggttgc ttcatagata ttgacttggt cgatgacctg
    16801 attatctatg ataccaattc caatcgacaa ggcttggtct tgaacgactt ggaaaatctc
    16861 cagatgtttc tttttaggaa ttttcttgct gtcgttgaga cctttaatct tacaattttt
    16921 aggtaaaata acggccgcag cgactacagg accagcaaga ggaccacgac caacctcatc
    16981 aatacctgca attaaggtca atccttgctt ataaagttct ttttcataag aaagcatgga
    17041 ttccaagcgc aaattttcat ctaattcagc ttgaatggct cttttatgct tgctgatttc
    17101 cttttgaact cctgagcgat tatccttttc aagatctaaa aaaatagggc tttctaactc
    17161 cttgactgtc acaaggagtt ctttgatttc tttaatcgtt gccatcgagg ccttccaatg
    17221 tatctaaggt atagttaccg agtttgccat cacggacttc cttcacgaag agactgtaaa
    17281 aacggtcata gtcatcacgg aaaccgaggg cgcgggtcat atccataata atcacaggcg
    17341 cttcttcttc aattttcatt tgtttgaagc gttcagccag cttttctgga taatgttctt
    17401 tgaaataatt gataccaaaa atggtaacct catccatagg aagcaactga tccttgatag
    17461 ctccagtcaa tgccaactta agtgcaacag tttcatcctc aaacttaggc cagagaatcc
    17521 ccggtgtatc caagatttcc agatctttat tggttttaag ccattgttga ccttttgtga
    17581 cccctggctt gtttccaaca acagcaatct ttttaccagc caaacggttc atcagagttg
    17641 atttaccagc gtttggaatc ccaataatca tggtacgcaa ggtttcaatc tgaatcccac
    17701 gttctttctg gcgagcaatc ttatcagcca tgagcttctt ggccgcatct gttacaactt
    17761 ttacagtcac ttgctctttg gagttgatag ctagcgtctg gattccttgt gattcaaaat
    17821 actgacgcca ttccttggtc attgctggat cagccaagtc ggccttgttt aaaatcaaga
    17881 gttttggttt atcaccaaca atcttggtta acataggatt ttgactagat agaggtaagc
    17941 gtgcatcaac taaaatcgtc acaaaatcaa caaattttaa attctcctgc acctgtcgac
    18001 gagctttaga catgtgacca ggaaaccatt gaatagtagc cattgagttt tttcctttca
    18061 tagcaaggat ttagatcccc tattttattt tactattgtt tgaacataga gagttttcaa
    18121 atgaatgatg tccaaacagt tcttgtttta tgacatagaa atctacttta tattatatca
    18181 tgccttcttt attttgtccg atgatagcct tttaccctat atagaaaaaa gccatcaaat
    18241 gatgactttt cagagggagc aaaaaactgt tcccgtcata ttatgaacca aaagaactaa
    18301 actaccaaca gctaataggc ctaaagctgt aagtgcagaa gttgatgaac ctgttgcagg
    18361 aagagtttcc tgctgataaa taagagcata acttacttca ttatcagtag gaattcctgc
    18421 ttgaatcttg gtttgttctt catcaccttt ttaaacttta tttcctaata gtaaacacta
    18481 ttttttattc tcaaaaattg aacttctaaa atcatttgtt tggtcaagat aggctttaaa
    18541 gattgctctc ttcaacttat gaacggcact gtcgatttct ggatcataat tactccaacg
    18601 tggaccttca gcatccttga gaacagcttg gtccatgagg gcttgcaatt catccacatt
    18661 attgatagtc tttgtgccat agcttggcca tggttttgtc ggatctttaa aggtcacctg
    18721 cttcaagttt ccaaactgat ccactcgttc ttggtacata gctgtcttaa attctgccca
    18781 agttttatac ttaccgtcaa ataccttttt caaaacaagc tcatcggtca ccaagccccg
    18841 ttctttacca taaagattga tagtttgccc ttgttgttta gcatcttctt cgtattggtt
    18901 tgagatataa ggtaccattc catccttaaa gcctttggca gccaaaagtt cgtacgcaat
    18961 cctacgtccc ataaggtccc ctggtgtgcc tttctcactg ctgagagctg aatagattgg
    19021 ggcaaagagt ttgatggtat agtagccatt tcgttcatag tcgccagact ggtactcacg
    19081 agctgataag atgttgtggt caatcaaact ttcaaaacta ttcaggtttc gggcctctgc
    19141 ttctgtcaat tctttgacta cgttagtggc ataaacctca tttccatctg ctggatcttt
    19201 cacatacttg ttctcaattt ttctgagagc cgccattttc tggtaaacac ttagtttatt
    19261 aacgattgac tgcccttcga gatattccat catgtagata aggtcaaaca tgttatggac
    19321 atagttctga agatctgctg cgttttggaa tctctctgtc ggatccaaga cttgaagacg
    19381 ggatccctct gtactatctg attttgagtg tttcaagata gaattaatgg taatggttgc
    19441 gtcacttggt tggtcagggg cttgcaataa gccttttgcg aagaactctg gtcccaagcc
    19501 atttcttcga ccatagccac caaggtaaat atcctgatcc gaatcatggg tcatttcatg
    19561 ggtataagta atggctccat ccttgtccag catgcggtaa gacatatagt agacgccgtc
    19621 ccctgtagcg taggctccat gctggttgtg tactacctta ttgccaactg gtccaaagaa
    19681 gttcttcata gctggattgg agctatcaaa cttagcctcc gctgtagctt ttcctgatgt
    19741 tgtgtcatcc ccaaacttat aggcatcata aaggagaatc gtacgataga gtttttcgcg
    19801 tgactgttca tctaaaatac gataccaata atcgtagtga tctcgctgac gtttggctgt
    19861 ttcacgcgca ttttcttcaa caaaatcatt gagagccttg cccgctttat ggtcactact
    19921 gcggtagcga tcataagctc caaatcctag actagacatg gtcgagatga caaatacgga
    19981 tctctctggc aaggtcagga gaggcaagac catattgcgg tatttccatg tggcactcgt
    20041 gatacgatca taaacaccga tagaatactt ggtgccagct aacccttgct tcgttttcac
    20101 ctcttcgata gtggattttt cttcgacaat gtaagcctta gtctctgatt taaaccagtc
    20161 attattgctt gtatttggta aaaagacttt tcggtaatgt tccagcgtgc taaacaaatc
    20221 tgtcgttcca tgttgactgg caagactgat accataagta tcgacattat tcttagctag
    20281 aagattgtta aagccagatt tacctaactc aatcagagta tctagtgggg aagtatttcc
    20341 tttaccaaag aagtctggat gatacataac taggtctttg acattcactt gaccatagtt
    20401 aaagttatac caacgttcta aataagttaa accgagtagc aaggcttcct tattattctt
    20461 gattttatct acaagatagc ctctggtgac tgagttatcg ctagctagac cggcatctgc
    20521 tgacaaaagt tttttcaagc tgtctcccag attttgtttt gttttggaga actggtcttc
    20581 taaatagagc tcggttaact taacttctgg agaaatacca agtgtctttc tgatagcatc
    20641 tgactggtaa tcaagcttct gcaactcagg caagacttcc ttaacaatag agtctcgatc
    20701 atataagaat tggttaggag tgtagagaag tcccgtatta cctagattat attctgctag
    20761 attggcaaaa tcagtctggt acttgagatc aaatttctca gatgaatggt cattatagtg
    20821 aagtaagagt ttgtttgcag tctgtttgtt ggaaacaata tctgtgatta cttggtcatc
    20881 cttcatcata actgcagata agagttcttt ttggtataag agactgttct ccttaaccag
    20941 gtttccgtat ttgacgatgg ttgacttatt ataaaaaggt aacaattttt caatgttctt
    21001 ataaaccaaa gcacggttag cttgataatg ggctatctta gaaaaatcac tgtctttctt
    21061 gccacttgtt gagaggggtt ccaccgttgg tagagtgaga ggatttattt ctgctttttt
    21121 gcttacaatt tgagaagcat ctagcattgt tcctctttct tcaaaggatt ccttgctgac
    21181 gacctcatcc ttctctaaag tgacattgaa aactctatta gccttactac taaatgtatt
    21241 agctaccttc attcctgtat agtggtatcc agtgatggca tttccgtttg ttacattgac
    21301 atcgctaaga acattggtca attcaccagc gtgtttttcc tcaccagaag ttcgatccca
    21361 caaatatcct gccacaccag cgactttacc aaagtgcttg acattattaa tatcaccttc
    21421 cgcataactg ttctgtatat gcgcatcttg gtctactaga cctgcaagac caccgacagt
    21481 ctgatctgac ctattggtgt ttgacgaaat ggttactgtc gctttggatt tagcaataga
    21541 cgcatttttt cctgttaaat gaccgaccag accgccaata ttgtaggcat cagtcgtttc
    21601 ataggtattg acaattcttc ccttgaaact gctctctgcg atgcttgatt ggtctgcctt
    21661 agccaacaaa ccaccgacac cacgttctcc agccagaaca ccatcaacat gaacttgttt
    21721 aatctttgtg ccattcgtag cttcatttgc tagtgaacca atatcatttt tccctgaaat
    21781 agcaacattt tttagactca gtttttctac tgtagcacca ctcaagtttt caaacagagg
    21841 ttttttcaaa ttatagatag cataattctt gccatctttt tcaccgatta aacgaccagt
    21901 aaaggtgtcc ttgatatagg atctttcatc aggaccaagc tccacttcgt tagcattcag
    21961 gctggccgct aaatgatagg ttccagaggg attttggttt atagctttga ccaggttgct
    22021 aaaggaagta aagtttgtat tttcctcttt agccttctta tcgaggtaga aggtgaaatt
    22081 gtcgacatat ttattttcta gttcttgctg gagtttctca gctttggctg tgattttata
    22141 aactgactgc ccatttcttt tctcttcagt tattgaagct actggtatat agacatcttt
    22201 aaatgctgaa gattttacct tgacaaagta agtatccgta ttctctggaa ttccatccaa
    22261 tgaaacatgt tgtctgtagg ttccattcgt ctgactatac aactcaatat cagaaacatt
    22321 tcttaattcg agttttttct ctggttctgt ttgtccattc tctgatgttg tattacctga
    22381 attttctggt tttgttgcag ttccgttttt ttctggttga tttgattctt caactggtgg
    22441 ttttgaatca ctaggtttat tggatacttc tccagtattt tcgttagcta ttttcccaga
    22501 gtttgtttgt gtttcttctg caggttgaac tggtttttct gtttcttgat ttgaggtacc
    22561 ttctactgtg ccttcatttg gatttactgg aacttcttct acagtttttt ctgaattttc
    22621 atttttagag tcattatgtt ctggtttatt tgattctcca actgaggttg tcgaatcact
    22681 aggattactg gacacttccc cagtattttc gctagatgta tctggtgata ctttctctga
    22741 attcgttgtt gattcttctg caggttgaac tggattttct gcttcttgaa ttgaggttcc
    22801 ttctgtagta ccttcatttg gatttactgg tgtttcttct gttggtttta ctggaacttc
    22861 ttcagttttt tctggacctt gttctttggt cttctcaacc ggagtttcag gttttacttg
    22921 ctcaatatta cccttatatt ctggcaatgg ggctacctgc tctggttcac ctttatcact
    22981 taccacagta tctggcgact ctggttgaac ctcagtctca cctttgtcgg tcacaactgc
    23041 ttcgggtaat gtcggttgaa cttctggttc gcctttatca cttactacag cttcgggcaa
    23101 ctcaggctga attgcgggtt caacaataga tccagactgt acgtccttat gttctacacc
    23161 agtctcaggt tgttccttta taacttgagt ttttttagta cctttttcga ctattcttgg
    23221 actaggcgca gtcgttgaag ttgaaacaat ttctcgcgaa acttcttcct tgtttacaga
    23281 gaatattctg acgatttcaa ctttcttacc taatttacct tcttgtttta ctcttacagt
    23341 tccttcagct aaatcaggat tttcttgaat ttcttcttga aaatctattt ttgtctctat
    23401 agtttcctca cgatataaga gttcaggttt gttcaattga cctgataaaa cttcatcctg
    23461 tggatttaat gtatttaccc cagtcttttc ttttggagaa atcttctcct ctttcttcgt
    23521 ttctagattc ttatgttcgg ctaattgttc ttgagaatct gaagattgtt tctcttcttt
    23581 tcttggattg attaattcag tagagaaagg tttttcaact acttgaactt ctgtcggctt
    23641 agttgaagaa acaggtgttt gttcctgaat agcttgtact gttgatggat ggtctacaaa
    23701 atttggtgta acattataat ccaccttttg ttgttttgta ggagtggctg ctgatttttc
    23761 ttgattactt acttcaaaat cagaagtcgt ttttccctct ttgatatatc caatataagt
    23821 gtaacctgaa atctctttag gaagaggtaa tttttctcca gaggtcaatt catagtccgt
    23881 attgtaattt agcaaaagat gattttctaa agcatggact gaaactaaga caccatttcc
    23941 cattcccgca accaatacta aatgtaatac cgttttattc ttaacctttt tcttggaaac
    24001 agcaaaaatt aaaattccca gagcagctaa gctagcacca gcaactaggg cttgcctctc
    24061 attcttgctt ccagtatttg gtaattccgc cagttgattt tgagaattta acttataaac
    24121 aagataataa gtttcatcat cattctccac gtatgtcgga atatcataga caagctgctt
    24181 cttttcttct gatgatagct ctgaatctgc cacatattta tagtgaactc ccgcagtttc
    24241 ttgagcatcc acagatgaac tagctaatac agacataaaa aataaacttg aaatcgttgc
    24301 agatacaagt cctactgata attttctaaa tgaaaaacgc tcttgttttt caccaaaata
    24361 cttttccatt attcctcctt gaaataaaat ttatatatgt tacaaagacc tttattatat
    24421 tagtgtatta tctattatct atagaaaagg cagtatacct taattatact cttaatttac
    24481 aaaaaagtct taaaattgag atgcgctttc atactttgtt ttatattatt tgggggtaca
    24541 ataacaccta ccatgaaatt tacacggtag gtgttactca tatcactaat cgttctaaaa
    24601 atggtttgag gcagttgagg agaattcctt ctatccagct tccttgtgct gatgagcgat
    24661 ggtcttcctg caggcttttt tttagaaaat ctcggacttg ttctggtgcg atttcaaatt
    24721 caaaggcttt cattttatag aaaaagtcga tgagatgatc tgacaggtat tcagttgaaa
    24781 agggtacttc accacttttt cgatattcta ataagagtct agaaaatcga gctttttctt
    24841 caggaagctc acgaaaatag gaattgagga tccaagtctg cttctgtttt ctttcaattg
    24901 gatcctgact ggcaattcgt tggtcttttt ccagctcttt ttggtattgt ttggccttga
    24961 tagctcgttc tgctctattt ttaccaaaaa gaattttttc ccacttgcgt tcttcttgag
    25021 tcagggtctc tgtaaagcca aagtaatctt gataagcacg ctctgcgggt cccatggcta
    25081 gaaccagatt gtctgcatat tgcttggcga ttttatccct cttcttgcgt tctttctctg
    25141 cctggatacg gagttcttgt tcgtagtcaa ttttctcctt gcctagcttg acaaggtaga
    25201 gttggtcatc cgatttccca agtaaaaagg gtttgataca cttttcaagg acttcttcca
    25261 tccgagcctt tttctttggt tccgccttgg tccaacttcc tccctgaaag acttctagga
    25321 aaagctggta gtctctctca ggcgcaaatt gattgccacg attgggtttg aaaacacctt
    25381 tttcccagag ccattttaga agtcgctcgt caaagtcact tttattgacc ttgatttttt
    25441 cctttttctg agcttttctg gttagatttt caacctttct gagcagtttt tcttcctctt
    25501 ccaattgctg gtcaaggggc aatcgatgaa aatgacgaac acagtcgcta ccaattggaa
    25561 agaggcgttg gcctgtgaca ccgttaaaga gttcataagc gtatttgatg gcatttcgac
    25621 agacacaatt gctacggcca ataccgttaa aaatcaagga aacttcattc cattccttgg
    25681 tagcttgttc ccaagtatcc gctttcgaag cctgtaaaac tgcatcgtgc agggattttc
    25741 taactggaag tgtcatgagg tctcctttct aatactcaat aaaaatcaaa gagcaaacta
    25801 gaaagctagc cgcagtcagc tcaaaacact gttttgaggt tgtagataga actgacgaag
    25861 tcagtaacca tatatacagc aaggcgaagc tgacgtggtt tgaagagatt ttcgaagagt
    25921 ataagttata cttttacaac ttgaacctcg tctttaccga gtaaaatcaa gtatttttca
    25981 atattttcaa tcgaataggc tcgtgataaa gcctcttcgt atagagctaa ctgaccacga
    26041 tagcggtcta cgagttgact tggttcatca tagcggtctg tcttgtagtc gaacagaaca
    26101 attttgtttt cgtaaagcag atagccatca aggataccac ggacaacaaa gtcttcctga
    26161 ctcttttggt ctcgtttgag catggagaaa ggttgctcgc gatagagatg gtcggtatta
    26221 gcaagaattt cctgaccgag tgctgtgtca aagaaagcaa gaattttatc aagattgatc
    26281 ttgtctctga cagcttggct agtttgaact tgtttgagtg tttctgttaa gctagcaagg
    26341 gttagttgct ggctgaggtc aattatctgc atgagttcgt gagtagcact accaatctca
    26401 gctccagtta ccttttcttt ggttgaaaaa tctggcaaat cgaagctgat tttcttgcct
    26461 actgactgac cttgaccagc aatctcgaca ccttccatat ccataactgg ttcgtagaat
    26521 ttcttgattt gacttggggt ttgaacacta ggaagttcaa tagctgcgcg gtgaagagta
    26581 ttataaactt ccacctcctt cagcatttcc agagcttctt tgatggtatc tgactgacga
    26641 ttgtctgctt gggagctatc ttggagagga ctcttggttt ccaactctcc gatagcttct
    26701 ctggtcaact gatcttcgtc aataaaacga taactaaagt tgagcttgtc cttagtaaac
    26761 actttactga tagcccaaag ccaatcttgg aaattccgtg cttgcagtct agtattgcta
    26821 tttagtttcc catttttggc tgctgggtat tccttggatt ccagcttttc acgagaaccc
    26881 ttgccgacaa gatagagctt tttctcagcc cgcgtcatag caacatacag caaacgcatc
    26941 tgctcagaat agcttgctag ctgtaattcc tcttcgttct gcctataggt cagactagga
    27001 atggagagtt tgatggtttt aggatagtgg tcttctactg cccctgtctc catcttggca
    27061 atatatttga caccaagacc attctgacga ctgagaatga cttctgacat agagtcttgc
    27121 ttgttgaaat tttgatccat attgaggata aagacgtaag gaaactccag ccctttactc
    27181 ttgtggatgg tcatgagctc tactgcatct tttggcggtg cgacggccac gcttgccaaa
    27241 tcgtgctggg cttctaagac ttggtcaatc atacgaataa aacgcgacaa acctttgaaa
    27301 ttgctctttt caaattgatc agcacgcagt gctagggcat agagattggc ctgcctagca
    27361 ggaccattcg gcaaagcccc aacatagtca taataaaaac ggtcgttgta aatcttccaa
    27421 atcaagtcat agagagagtg ggttttggca tacaagcgcc aagaagctag gatatccatg
    27481 aattgcttta gtttttcaac tagagctgtg tgaatcaagc ctttttgact acttgccatt
    27541 ttttgtgcat tgaccagttt ctcatagaga ttttcgtgga ctttatcctc tgctttctga
    27601 agggacaaac gtgctagctc atcctcatca aaaccaaaca ttggagactt cataagggca
    27661 accaaggcgt agtcttgcag gggattgtga atgacacgaa gagtgtctag catgacttgc
    27721 acttctaggg attggagata attgttttgc tctccgtcag ttttgacagg aatcccgtac
    27781 tcagacaggg cgaggagaat ctggtcatta cgactgcggc tggaggtcag aagggcaatt
    27841 tccttaaagg caacaccttt ttcttgatga agtttcagaa tctccttgat aactaagcgc
    27901 atttcgcctg ttagtttcgt ttctgtttga ctctcttctt cctcacctgt atcgtccttg
    27961 tcgtagagga gaaattctgc cttgttgtct ggattgggag tcagtttggt attggcaaaa
    28021 acaagctggt gcttgttatc atagttgatt tcaccgacct cttggtccat gagacgttca
    28081 aagacatcat tggttgctga cagcacttct gaactactac ggaaattttc cttgaggaga
    28141 atgagcttgc cttcttgggg attttgcgca tagcgttgga atttctcatt gaaaatctgc
    28201 gggtctgcct gacggaaacg atagatggat tgcttgatat ctcccaccat aaagcgattg
    28261 tggccattag acaacaattc cagcatccgt tcttgaatat ggttggtatc ctgatactca
    28321 tcgaccatga cttcatggaa gcgctcctga taagactcac gaacttgtgg gaaattctct
    28381 aaaatctcaa tggtgtaatg gctgatatca gcgaattcaa aggcattttc ctggcgtttg
    28441 cgctgacgat aagcctccac aaaatgactc ataaaggttt ggaaggtttt agctagtttc
    28501 catgtgtctt gatgataacg ttcttgatag tcgagaatgg ttatctggtc tgataattgt
    28561 cctagtttag caaactgggt ctttctctct tcgttgtagg catcagccag gggcttcaaa
    28621 tcagccttac gactggcatt agtcagagct cgaccgtttt tctccttaga gatggcgaca
    28681 acacgcgcaa gcactgcctg ataagcctga ctatcggact cctgatttag ggagcaaatt
    28741 tcatccagaa ttaactgaac attttctaaa taggcagcct ttgcaaactc cttggcatcg
    28801 ttatccagat ggtaacggaa aaagctttcc aaatcccaaa gggcttgttt gatttgctca
    28861 gtcagttttt ctttttcact ggtaaaatca gctttctcaa atcctttgag gaaagattca
    28921 ctcagccact tttgaggatt actggtggat tggaggaagt catagatttt atagacctgc
    28981 tggcgcagac cccgttcgtc cttgccacgc ccagcaaagt ttttcagcaa atgactaaag
    29041 gtctctttct gtttaccttg gtaatgcgct tcaaagacct catgaaagac ttcgttttcg
    29101 agaagaagtt gctcgctttg gttttgtaaa atacggaaat taggtgcaat atcaagcaga
    29161 taaccatgtt tgccaaggaa tttttgtgtg aaagagtcca tggttccgat ggcagcgttt
    29221 ggtaggtctg ccaactggcg tcccaagtgt tgtttgaggt cgacatcatc tgtttcttgg
    29281 attttcttgc tgattttttt ctctaaacgt tctttaagtt cagttgcagc cttgacggta
    29341 aaggttgaga taaagagttg agaaatttcg acaccacgcg ccaattggtc cagaatgcgc
    29401 tctgccatga caaaggtctt tccagaacca gccgatgctg agaccaggat attctgacca
    29461 gaagtgtaga tagcttcgat ttgctcggca gttttctttt gttccttgct cgaatttgct
    29521 tctgcttctt gcagtttttg aatctcctcc tcacttaaaa agggaataag cttcatcgat
    29581 tcaactcctc tcttattttt tcaagccaag cttgcttgag tttttctccg accagacgct
    29641 tgccatcagc taggtccaac ttttctagga aacgggcttg gcccagatgg taattggctt
    29701 caaagcctgt aatagcctga tgttgctgga cgtatggggc aatgcttctg ccattttcag
    29761 tataaggatt gatggcgaac cggcctgcta aaatcttctc agcagctttc ttgtaaagat
    29821 aggcattgta gtccagtagg agctgaaatt cctcatctgt cagttgattc gccttgtttt
    29881 tgttataaaa ttcgcctaaa taactgcttt ctttttccaa gaagagccct tggtatttca
    29941 tagatttgct ggcttctacc actgctcctg ccagactttt taccgccatc agagattgga
    30001 caggttcagc catttccaag tacatggcgc cgaaaaagtt ctgctcccct tctcttttta
    30061 gggcagcaag ataggttggt aactgagaat tgagcccatt aaagaaatga ggaaactgga
    30121 actgagtcag actggatttg tagtctacta ctcctatcgc tccattagct ttcaaacggt
    30181 caatccggtc caccttgcct cgtacaaaga cactgcgtcc attgtctaat tgaataaagg
    30241 cttggtcttt tccaccaaaa tttgcttctt ctttgattgt ttcgatggct ggattgtgtc
    30301 ggagaatatg tccagttgtc cgtgcaacat caagcaaaac ttccttggta aactgggctt
    30361 ccaaactttc ttgataaata gcttcaaatt cgcgttcttg actggtttct tgaatagctt
    30421 gttctagacg ttggtcaaag gaatcttcat taggcaactg caaggcgcgt tcaaagatac
    30481 gatgcaagaa attcccgtga ctacgggcat caggacgcaa acgtaattcc tcctgcaagc
    30541 ctaaaacgta gcgtaggaaa taactgtatt cattgcgata aaactctgtc aaacccgacg
    30601 tagacaggta aaactcctgt ttggcaggat agagagcttg caaggtgtcc ttggctaagg
    30661 tcttgctgct tggactgatt gggatggctg gattttccag accttgctga tctagttttt
    30721 tacctatgac acgcgacaga accttgacaa aagtcaaatc ttgctcagta tcgctcatct
    30781 caccctgctg gtgataggca accagactag acaaaagact gtgataggac cccatatcct
    30841 ccttagacag tcctttgtga ttcatcctct tctctctccg cctaaatcca aaatggatca
    30901 actcttgaag ataggcagat tccttacttt cactttcgtt aaaaaggctt ggagccgaca
    30961 agaacaactg cttacgagca gaattgacca aggaaagcat agtgtagcga tttttcttga
    31021 gattttcact gctggcaatc agtaattgaa cgccttcttc ggtcgtttgg tttaggtttt
    31081 gcctttcttc atctgtcaga agactggtgt tttgagaaat ttttggtaaa ttgtcctgag
    31141 ttagtccaat agcatagaca aagtcagcag tcaatggtgc aatcaaatcg taactctgca
    31201 ccagaacagt gtccactgtt gctggaatgg tacggtattg ggacaaactc attccagaat
    31261 ggagcaaggc taggaagtct tccagactaa cctgtgaacc agcaaaaaca gtcgcaaatt
    31321 gttctaaaac atggcagaaa gccttccaaa cttcggcttg tctttcctgt tctacagctt
    31381 ccaaagtggt tgtcaaatct tgtaactgct tggtcacagc tccttctttt agaaagacac
    31441 tccatttttg taggaggttt tcagcctttt gttttcggct ggcaaagagg gtttcaagag
    31501 gtgctaaaat tctcaggcgg aggacattca aacgctcaag attaaatttt ccatggtggg
    31561 atttggtgaa agtttgctga aaggctggca agccattgat accaagatag cggatatatt
    31621 gctcaaaagc atcaatatca gactgactga ggtcagtata caaatcagtt ctaagaagat
    31681 taatcaaatc ctcctgacga aaacggtaac gttttaaagc taaaatagac tcgacaaact
    31741 gagtcaaggg atgatgagcc atggcttcgc ttctaccaag ataaaaagga atctgatact
    31801 ggtcaaaaat ggttttgaga gataactggt aagaagctac atcccccaag agaatacgaa
    31861 aatgcttgta gctcaggtct gagttctcat gtaatttctg acgaatgcta cgggctacta
    31921 gctccaactc ctccttttgc gtcaaacaag accagatttg taaattttca cggtctttct
    31981 catcgacatc caaagcgagt tctgaaaagt cataagaaga ctccaacaaa cgagaggcct
    32041 tgtcaaaact atccatcttc tcatgagttt gagaacagtc ctgagcaggc gtttggtatt
    32101 tagaagccag atgatggaga aattttacgc tggcttggta gagattgccc tcgctaaaag
    32161 gactggtata ggctttctta ctagcataag ccccgataac aatctcaaca cctttgccgt
    32221 gaagtaagtc cacaacccgc tcttcctcag cagaaaaacg agtaaagccg tcaatgacca
    32281 aggtgatttg attaaaatca ctacttacct tgtcattctc aatagcctca atcaaatggg
    32341 acaactgact tccctgggct aactgacctt gattaagata ggctgttact ttctcaaaaa
    32401 tcaagagtaa atccgccctc ttatcctcat ctgttaaatt ctccaagtcc aaaaaactca
    32461 tctgagattt ggtcatctca tggtaaagct caattaactg ctggatcaat tgaggatcct
    32521 gcttaatagc gccataaaca cgcaagtcct tgggatcgag ttcggcaagg catttgtaaa
    32581 aggccaaccc aagaccgata tcatcaagag tagttttaac tggtaaatca ttcaagacca
    32641 gataacgagc catttgagca aagcgcgtga cggtaatcga aaaagaagcc tgctgggaca
    32701 agcattccag cacggcgcgt tccttttcaa aagaaagaga gttgggggca atgtagaaga
    32761 cccgcttgcc agctgcaact agctcttctg cctctcttgt tagaatttct gtcaaagaag
    32821 ttcgaatatc agtataaagt aatttcatct cagcctcgtt ggaatttttc atcaccctat
    32881 attataccat gattagcctc gtaaatctgt taaaatattt aggccatcct ttcttttctt
    32941 catcatctgc taaatcttaa atacttagct ttacttgtat tagatagaat aagtctggct
    33001 actgaaaatc acataataaa aaagcctcgg taacaaggct ttgagtttta tgattgtttc
    33061 ttaggtacgg aatacacttc aatgtgttgt cccagtatct taatgtcgac tggtagattg
    33121 tctgatttat cgccatcaac atcggactct aattcgatat cagaagaagt tttaatatta
    33181 cgtgccttta tatattcaat attcttgata gaatgattga actatagtaa attgaaacta
    33241 taatagtaca ccgtggatgc taaaatattt ctagaaatta atttgatttc cctaatcaag
    33301 ctgttcgtat cttatttcaa tcta
//
LOCUS       DAVBBS010000018        32825 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVBBS010000018 DAVBBS010000000
VERSION     DAVBBS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32825
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.018"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(321..1010)
                     /gene="rplA"
                     /locus_tag="VQU32_000831"
     CDS             complement(321..1010)
                     /gene="rplA"
                     /locus_tag="VQU32_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET2472309.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMGLVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(1217..1642)
                     /gene="rplK"
                     /locus_tag="VQU32_000832"
     CDS             complement(1217..1642)
                     /gene="rplK"
                     /locus_tag="VQU32_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET2472310.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAVRMIEGTARSMGFTVID"
     gene            complement(1765..2481)
                     /locus_tag="VQU32_000833"
     CDS             complement(1765..2481)
                     /locus_tag="VQU32_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET2472311.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKAKNEDGAAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     VIGTNGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYFGFEGGDYVD"
     gene            complement(2484..2954)
                     /locus_tag="VQU32_000834"
     CDS             complement(2484..2954)
                     /locus_tag="VQU32_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET2472312.1"
                     /translation="MCLICQRIELIKKGENPYFVKELETGYLVIGDHQYFAGYSLFLA
                     KEHVSELHHLEKKTRIRFLEEMSVVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     RGDMNGYGLNGRGPVWWIPFEEMTAEICQSKPDEIKRLVERLSSEVDKLLEIKE"
     gene            complement(2956..3993)
                     /locus_tag="VQU32_000835"
     CDS             complement(2956..3993)
                     /locus_tag="VQU32_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HET2472313.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVLGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAMLLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTNEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLKSALVD"
     gene            complement(4091..5416)
                     /gene="brnQ"
                     /locus_tag="VQU32_000836"
     CDS             complement(4091..5416)
                     /gene="brnQ"
                     /locus_tag="VQU32_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HET2472314.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(5587..5679)
                     /locus_tag="VQU32_000837"
     CDS             complement(5587..5679)
                     /locus_tag="VQU32_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2472315.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(5722..5874)
                     /locus_tag="VQU32_000838"
     CDS             complement(5722..5874)
                     /locus_tag="VQU32_000838"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2472316.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            complement(6034..6612)
                     /locus_tag="VQU32_000839"
     CDS             complement(6034..6612)
                     /locus_tag="VQU32_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET2472317.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPCT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(6666..8066)
                     /gene="pepV"
                     /locus_tag="VQU32_000840"
     CDS             complement(6666..8066)
                     /gene="pepV"
                     /locus_tag="VQU32_000840"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HET2472318.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLTDLQAKLDAFVAEHKLRGELQEEAGQYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(8079..8684)
                     /locus_tag="VQU32_000841"
     CDS             complement(8079..8684)
                     /locus_tag="VQU32_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET2472319.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDLKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            8855..9655
                     /locus_tag="VQU32_000842"
     CDS             8855..9655
                     /locus_tag="VQU32_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2472320.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(9837..10670)
                     /locus_tag="VQU32_000843"
     CDS             complement(9837..10670)
                     /locus_tag="VQU32_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET2472321.1"
                     /translation="MNHKIAILSDIHGNATALEAVIADAKTQGASEYWLMGDIFLPGS
                     GANDLVALLKELPITASVRGNWNDCVLEALDGQYGLEDPQEIQLMRMTQFLMERMDPA
                     TTVWLRSLPLLEKKEIAGLRFSISHNLPNKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELVNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKNFFDF
                     L"
     gene            complement(10660..12507)
                     /gene="uvrC"
                     /locus_tag="VQU32_000844"
     CDS             complement(10660..12507)
                     /gene="uvrC"
                     /locus_tag="VQU32_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET2472322.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGIHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVAAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGRPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQRKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            complement(12547..13248)
                     /locus_tag="VQU32_000845"
     CDS             complement(12547..13248)
                     /locus_tag="VQU32_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET2472323.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKMGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(13327..14559)
                     /gene="murN"
                     /locus_tag="VQU32_000846"
     CDS             complement(13327..14559)
                     /gene="murN"
                     /locus_tag="VQU32_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HET2472324.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSEYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(14564..15784)
                     /gene="murM"
                     /locus_tag="VQU32_000847"
     CDS             complement(14564..15784)
                     /gene="murM"
                     /locus_tag="VQU32_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HET2472325.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(15896..16675)
                     /locus_tag="VQU32_000848"
     CDS             complement(15896..16675)
                     /locus_tag="VQU32_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HET2472326.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(16744..18405)
                     /locus_tag="VQU32_000849"
     CDS             complement(16744..18405)
                     /locus_tag="VQU32_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET2472327.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPEHIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKILSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(18580..20802)
                     /gene="recJ"
                     /locus_tag="VQU32_000850"
     CDS             complement(18580..20802)
                     /gene="recJ"
                     /locus_tag="VQU32_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET2472328.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKTYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            21079..21837
                     /locus_tag="VQU32_000851"
                     /pseudo
     CDS             21079..21837
                     /locus_tag="VQU32_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            21877..22641
                     /locus_tag="VQU32_000852"
     CDS             21877..22641
                     /locus_tag="VQU32_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET2472329.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTELLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            22654..23331
                     /locus_tag="VQU32_000853"
     CDS             22654..23331
                     /locus_tag="VQU32_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2472330.1"
                     /translation="MIDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILAHIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCMGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQTFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            23341..24000
                     /locus_tag="VQU32_000854"
     CDS             23341..24000
                     /locus_tag="VQU32_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2472331.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNTVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLCILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            24295..25485
                     /locus_tag="VQU32_000855"
     CDS             24295..25485
                     /locus_tag="VQU32_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HET2472332.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(25806..26687)
                     /locus_tag="VQU32_000856"
     CDS             complement(25806..26687)
                     /locus_tag="VQU32_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HET2472333.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(26828..28156)
                     /gene="vncS"
                     /locus_tag="VQU32_000857"
     CDS             complement(26828..28156)
                     /gene="vncS"
                     /locus_tag="VQU32_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HET2472334.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGATMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(28153..28809)
                     /gene="vncR"
                     /locus_tag="VQU32_000858"
     CDS             complement(28153..28809)
                     /gene="vncR"
                     /locus_tag="VQU32_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HET2472335.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASIAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(28858..28941)
                     /gene="pep27"
                     /locus_tag="VQU32_000859"
     CDS             complement(28858..28941)
                     /gene="pep27"
                     /locus_tag="VQU32_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198524447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HET2472336.1"
                     /translation="MRKEFHNVLSSGQLLADKRPARDYNRK"
     gene            complement(29121..30500)
                     /gene="vex3"
                     /locus_tag="VQU32_000860"
     CDS             complement(29121..30500)
                     /gene="vex3"
                     /locus_tag="VQU32_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HET2472337.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(30552..31199)
                     /gene="vex2"
                     /locus_tag="VQU32_000861"
     CDS             complement(30552..31199)
                     /gene="vex2"
                     /locus_tag="VQU32_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HET2472338.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKDTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(31212..32489)
                     /locus_tag="VQU32_000862"
     CDS             complement(31212..32489)
                     /locus_tag="VQU32_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2472339.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITSYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         9263 a         7521 c         6103 g         9938 t
ORIGIN      
        1 ttagaaatcg atttggctgc cctgatcgat tcgtcctact cttatttcat tttactataa
       61 ttgatagtgg tcgccccagc cagatacctt atccgctata taggccttat tatcatgttc
      121 ctgttcgtag agccacgatt tcgctatccc ttcctctgac gattcttatt tcctgcgtca
      181 gacttgggaa tcgctattgg gttcaccggt agcaggtgcc caccgaggac ttacacctca
      241 gatatacgac atgcccgtcg tacatcaaaa aaagttgaga catagcgtct tcaacttttt
      301 tacattactt taactactga ttaaagtgag tttacgtcaa ctttgatacc aacaccttga
      361 gtagttgtga tagtcaagtt tgttacgtaa gttcctttag ctgtagctgg ttttgctttt
      421 tggattgttt cgttgaaagc tttaaagttt tcaaccaatt tttcagcttc aaatgatact
      481 ttaccgatga ttgcttgaac gttacctgca cggtcagcac ggtaagtgat tttaccacct
      541 ttagactctt caactgcttt agcaacatcc attgttacag taccagtttt agggtttggc
      601 atcaagttac gtggtccaag gacacgtcca agacgtccaa caagacccat catatcaggt
      661 gtagcgataa ctacgtcgaa gtccaaccaa ccgtcgttga ttttagcaac aaggtcatct
      721 tcaccaacaa agtctgcacc agcagctttt gcttcttcag cttttgcacc acgtgcgaaa
      781 acaagaacgc gtgaagtttt accagtaccg tttggcaata ccattgctcc acggatttgt
      841 tgatcagctt ttttaacgtc gatgttcaag ttgtaagcaa cttctacagt tgcgtcaaat
      901 tttgcaaagt ttgtttcttt tgcaagtgct acagcttctt ctacgctgta tgcttttgtg
      961 ctgtcgattt tctcaagagc agcacgaagt tgtttgcttt ttttagccat tttctattct
     1021 ccttgtaagt ggttcaatcg attttcatct cccacgtcac ttctcgaaat gatgaagtct
     1081 tgcgggtttt cagtctatta gattcttttc ctacggcctt aagctccctt gctgtaccca
     1141 agtacaagca tcagtcactt gcctagtatg aaaagcaact aatagactgc tgtgagattt
     1201 tctactgtgt tattgattag tcaataacag tgaatcccat agaacgagca gtaccttcga
     1261 tcatacgcac tgcagactct acgtttgctg cgttcaaatc tggcatctta gtttctgcaa
     1321 tttcttgtac ttgtgcacga gtaactgtag caactttagt tttattaggt gtacctgatc
     1381 ctttttcaac acctgcagct tttttcaaaa gaacagcagc tggtggtgtt ttcgtaacga
     1441 aagtaaatga tttatcttcg taaactgaga taacaactgg aatgatcata ccagcttggt
     1501 cagctgtacg agcgttgaac tcttttgtga atcccatgat gttgatacca gcttgaccaa
     1561 gagcaggtcc aaccggtgga gctggtgtag ctttaccagc agggatttgc aattttacaa
     1621 gtttttcgac ttttttagcc atttttaaat cctcctttgt ggttttggcg gtaattagag
     1681 atttttacct cccacaagta tgtttttcac atacccatct attatacact atttatctgg
     1741 aattgcaaga gaaaagttta ttttttaatc gacgtaatct ccaccttcaa agccgaaata
     1801 ctcttccaaa ctgaggccac tagctgcaat ctctttagcc tcttttccga cataacgaag
     1861 gtgccattcc tcagccatat agcctgtttc cttttcctta cctttgagat aacgtacaac
     1921 aaagccataa tcagccgcat gatccaagag ccattgggcc gctttttctt ctgtcaccaa
     1981 atcaccattt gtcccaatca catcaaaggc taaacctgtc tggtgctcgc tatagccagg
     2041 acgggcagaa taacggtcgg cagcttcttt cccatcttga ttgacataat cttgatagag
     2101 cttggtctga gtttcataac ttctaaagcc actgtaatga tcgctaatgg ggaaacctgc
     2161 ctcttgcata gctttgatga gcttgaccaa ctcagccttg gctgttggat tttcccctgg
     2221 attgtagtct tttgacaatg gatagtgttt gttggctacg atgatttcat cgtatttccc
     2281 ttgaatgctg taatagtctc ctttgttaac cacttctgct tttttctggg cagctccttg
     2341 agacttactt cctactgttc catcagcttt agcagtttgc tctgtcttag ctgctccatc
     2401 ttcatttttt gctttttctt gtgaacaagc tgctagactc aaggctagca aggctgtcaa
     2461 gacaaggtat ctttttttca tctttactcc tttatttcta atagtttatc tacttctgat
     2521 gataaacgtt cgactaatct tttaatctca tcaggttttg attggcagat ttctgctgtc
     2581 atttcttcaa agggaatcca ccatactgga ccacgtccat tgaggccata accattcata
     2641 tcacctcttc gtctaggaaa cagatgccaa tggagatgag catcgccatt tcctagtagt
     2701 tcgatattca ttttctcagc agcaaaggcc ttggcaactg cctcttggac cacactcatt
     2761 tcttctagaa aacgtattct agttttcttt tctaaatgat gcaattcgct gacatgttcc
     2821 ttggctagaa agagactata gcctgcaaaa tactggtggt ctccaatcac aagatagcct
     2881 gtttccaact ctttgacaaa atagggattt tctcccttct tgatgagctc aattctctgg
     2941 caaatcaaac acatattagt ctaccaatgc actcttaaga taggcatcga ccattcgttc
     3001 tgttgcgacc accgagtcaa tatgagtgcg ctcataagaa tgactagact cgatacccgc
     3061 accaagaagg gcatgcttga cctctgcccc tgcagacata gccgctgaag catccgaacc
     3121 ataaaatgga tagatgtcca gcttaaaagg aatatcttgc tctttagcca aggcaaccaa
     3181 gtgttgacgg aagtcatagt gataaggacc tgaggcatcc ttgacacaga tagatactgt
     3241 gtactcattt gtctgctggt catctcccat agctcccata tccacagcca gatattctac
     3301 tacctgagca ggaatgttag aattagcacc atgtcctact tcttcaaaaa ctgaaaaagc
     3361 aaaatgagtt gttactggca attcaatctt ctctttctta taaatacgaa gcaggttgag
     3421 caacatcgct gcgctgacct tatcatccaa atgacgagac ttgataaaac ctgtatcagt
     3481 cacaacagtt cgtggatcga agctgataaa atcaccgacc tcaatcccaa gagcacgagt
     3541 ttctttttca ttgcttactt ttacatccaa acgcacttcc atattgtcct gcgtgcgttc
     3601 tgcagttcct gcatccttgt agacatggca agaagtttgg tggatgagga tggttcctaa
     3661 taccttttga cctgtgctag ccacatgaac ggtacagttt tcaccttcaa tcatgttcca
     3721 aggaaaacca ccgatacggt ccaatttaag acgaccatct ggtttgacag cacgaacaat
     3781 agcacccagt gtgtccacat gggcagtcac atagcgatgt tgctcatcat tttgaccttt
     3841 gatggtcaca ttgacaccac ccttggctgt gcgaactggc tggtaaccaa aaccttctag
     3901 agtcttgact aaataattcg aaatttcacg agtgaagccc gttggcgatg cgatggctgt
     3961 cagttctttg atatattcta ctgtttgatt catttcttca cctctttcgc tacctattat
     4021 aacacattta tcatcggata aaaaaagaaa atcactcctg tagacttggc tacaaaagtg
     4081 atttaagtga ttattccatt tcaaaaacat cgctttcttg cttgtttggt agaaccaatg
     4141 agagcaagat tccaacaatg gctggcacca accatgggag agatgccttg gcaaaaggaa
     4201 gaacgctaac aagatttgca agaaactcaa ccttaaacga gcttcctagt acgcttgcaa
     4261 tggcaataac tgtaaccaca gcaattgtca actgcatacc tggttttgaa agggcaacaa
     4321 atttgttgac aatgacaatc ataacgatag caatcgtgat tgggtacaag ataaccagta
     4381 ctggaattga gtacttgata atcgcatcaa gacccaaatt ggcaatagca aatccaatca
     4441 aggtaaaggc tgtcgcataa accttgtagc tgatttgtgg gaagcgctca ttaaagaact
     4501 cagctgttga cacaatcaaa ccaacagtcg ttgtgaagca ggttacggta accatagctg
     4561 caaggaagag ttgagctgtt gagccaaaga tttcttgagt ggcttgtgac aagatgtaaa
     4621 cacctggtgt tccacccttc atcgcttcag ctggtactgg gaaatgattt ccaagaaaac
     4681 ctaaaccgat gtaaagagcg ctgaaggcaa gggcaacaac gataccaaca acccaaatag
     4741 ttgaaatgta ttctttctta cttgaaaatc caagttgttt caaggtttga actgcgatta
     4801 cgctaaaggc cactgaggca agggcgtcca aggtattata accttctagg aaacctgtac
     4861 caaaggcaga agcttgataa gcagctgaag cagcttgagg acttgttcca ccatatttga
     4921 tagctcccag aacgaccaag ataacaatca aaattgcaaa gactggcgtt aaaatacgtc
     4981 caatgcggtc taagattttt gatggattaa gcgaaatcaa ataggctgcc gcaaaataca
     5041 gaaccgtaaa tacaatcaag ccaagtcctt tatttgcatc cgacaaaagg gggctaatcc
     5101 ctacttcgta agctgttgta gcagtacgtg gggtagcaaa gaatggaccg attgacaagt
     5161 aaagaactga gaggtaaaga gtcgcaaacc aaggcgctat cttcgttgaa atctcgtaga
     5221 tatatccttt aggatttagc gttccaataa taagggtcaa gacggcgata ccaacgcctg
     5281 aaaagacaaa acctgcgatg gcaggaagaa aatgttctcc agatagagca cctagagaag
     5341 gcggaaaaat caagttcccc gcaccaaaaa atattccaaa caggagcaaa cctgttaggg
     5401 tacctttttt agccataaaa atctccttca tatttataaa atactgatct agtataccac
     5461 gattaggagt ctgaaaaaag catcacgagg caaatattaa atgttttcaa ttttcttaaa
     5521 aataagaatt atctaaaaat aaaactctcc tacccagtcc acaccaagta ggagaaatgc
     5581 aaaattttaa agttcttcaa aggctaacat gccaccctgg acattgataa cattataacc
     5641 ttgttctaat aggaattggc aagcacgcgc cgatctcatt ccagatttgc aaataatata
     5701 atgcaagcga tctttgtcca attaatcata actatcagcc aattgactaa gcggtaggtt
     5761 gtgggcacct tctaaatgaa gagctgcaaa ctcatccact tctctcacat ccactagaga
     5821 aagctggtcg ttttggtaaa gctggtaaaa tgcgtcaaag gctatttctt tcattctttt
     5881 tcttctaata ctcttcgaaa atctcttcaa accacgtcag cgtcgcctta ccgtgcgtat
     5941 gttaccgact tcgtcagttt tatctgcaac ttcaaagctg tactttgagc agcctacgac
     6001 tagcttccta gtttgctctt tgattttcat tgactataaa atggttttaa ttcttttttt
     6061 caaatctggc actacttctg cctcaaacca aggatttttg gccatccaga tttgatttcg
     6121 tggtgatggg tgaactagcg gaaaataggc tggcagatag tctttatagt gtttcaccct
     6181 ctccgttacc ttcccactga ttttctcctg taaatagtag gcttgggcat attgcccaat
     6241 caagagggtt aactgaatat caggcaattc ctgtaagagc tgcggatgcc atttttctgc
     6301 aaaacctgta caaggcggaa gatcacccga cttgccatgt cctggaaagt agaaatccat
     6361 aggcaaaaca gcaaaataac ctgaattgta aaaggtatct tcatccacac ctagccagtc
     6421 ccgcaaacgg tcaccacttt tatctttcca gtaaaggcct gcttcttgag ttttaagtcc
     6481 cggagcctga ccgatgatat tgatgcgagc agtttttggc gctgcaaaga gaggctcaat
     6541 gccacgctct gtatagctgg cattctgcga atccgccatg atagcctgtt tgattctttc
     6601 gatttgagac atctactttc cctccaaaaa gaagtccaag cataagatct cagacttcta
     6661 tcgttttatt tgatcaattc gtaaatagct tcggcataaa ttgctgctgc tcggaagaga
     6721 tcgtccaagg caataaattc attggcttgg tgcatggtat caattgagtc tgggaacata
     6781 gcaccatagg caactccgcg ctctaacaag cgaccaaagg ttccaccacc gatgacttgt
     6841 tcatgacctt taaggcctgt ttgtttttca tagacattca acaaggtttg cacaagtgga
     6901 tcttccattg gcacatagtg aggggtatga ccatgttctg atagactgac agaagcaact
     6961 ggcaagtttt ctaggattga cttgatttgt tctggacttg ttccttttgg atagcggatg
     7021 ttaagggcaa tggtattatc agcacttgtt tcatcgaagc ggaagacgcc ggcattcata
     7081 gaaagagcac ccatcttttc atccacatga gcaatcttga gattttcacc ctcatgatcg
     7141 ttcaagagaa ttttacctgc gatgtcaagg tagtctttgg ctggaccagc aaagccaaac
     7201 tggctgagga agagggcaag gtaagttgcg ccattgacac ctgaagcagg catagcaccg
     7261 tgggctgatt taccaatgat cgtcaccttg tattggccag cttcttcttg gagttctcct
     7321 ctaagtttgt gttctgcaac aaaggcatct agtttagctt gcaagtctgt caagtcacct
     7381 gaaacgactg ctgttgctga ttctggtacc atattttcac gtaaaccacc tgtaaagctg
     7441 tgaagacggg ctgcacctgt attttcacct gcaaagtgga ggtattccgt gatgtttcct
     7501 ttttcaccat tgatgattgg aaattcagca tctggtgaga aaccaaagtc tggcttggcg
     7561 agtcctacgt gctcgaagta gtagtccata tctgcccagc ctgattcttc gtctgttcca
     7621 acgatgaagc gaactttctt agaagttgga aggcccaatt ctttgatgat tttcaaacca
     7681 tagtagcaag ctgttgtagg tcccttgtca tctgaagcac cacgcgcata aaggcgacca
     7741 tctttgatag ttggtgtgta agggtctgtg tcccaaccgc taccagctgg aaccacgtcc
     7801 atatgggcaa agattccgag aacttcttct ccatcaccaa actcaaaatg tcctgcatag
     7861 ttatcaacat ttttagttgg ataaccatca cggtctgcga tttcaaggaa tttttccaag
     7921 gcttttactg gaccaggtcc aaatggatgt tgggcatcag ccttgctatc atcacgttct
     7981 gagttgattt ccaaaaggct aaacaagtca gccaagaggt cttctttgcg tttttctact
     8041 tctgctgtaa aatcaattgc tatcattttt tcttcttcct atcttttttc gatgatttca
     8101 tctactggca agcggtagct tggttccaat ttgtcgtctg tgtaacccac tgtgatcaag
     8161 agttctgggc ggaaacggtc ttcaatttcc aaaacctcat tgacttttga tttgtcaaaa
     8221 ccaaggataa tattggaacc aattccttgg tctgtaagag cgagaaccaa gttcatagca
     8281 accaaacctg cattgagtgc taggtagtcg ctgacttgtt gttcattgta gcgtgcaaat
     8341 tcagctggca aatttttcat gaagtattga agttgttctt ctgagaaatt gttagcacca
     8401 ccaactcggg caatcttacg agcgcgtttg gcaaggtctg tatctgtaaa tagggcaatg
     8461 gttacaggtg ctgatgatac ctgctcaaag tttgaaccat aagccaactt tgccagttca
     8521 gcatttttct cacgaacaac tacaaatttc caaggctggc tgttgtgggc gcttggcgcc
     8581 aaggttgcaa tttcgatagc cgtacgcaca tcttttagat caacaggctt atcagtgaaa
     8641 tgcttggtcg catgacgttt tttatttaat tcaagaaatt tcataatcga tttccttttc
     8701 taattctact gcttccattc taccataatt tgaaagagct tgcagggatt tttcatgatt
     8761 aagtttttct atcacagaca taaaagatat atattggttt atcaggaaag tttctgataa
     8821 actagcaagt actttacatt tgaatggaga cagtatgaaa aaatttagcc tattactagc
     8881 tatcctacca tttttggttg cctgtgggaa tcaagctaca cccaaagaga ctagcgctca
     8941 aaagacaatc gtccttgcta cagctggcga cgtgccacca tttgactacg aagataaagg
     9001 caatctgaca ggcttcgata tcgaagttct aaaggcagta gatgaaaaac tcagcgacta
     9061 cgagattcaa tttcaaagaa ccgcctggga aagtatcttc ccaggacttg attctggtca
     9121 ctatcaggct gcggccaata atttgagtta cacaaaagag cgtgctgaaa aataccttta
     9181 ctcgcttccg atttccaaca atcccctcgt ccttgtcagc aacaagaaaa atcctttgac
     9241 ttctcttgac cagatcgctg gtaaaacaac acaagaggat accggaactt ctaacgctca
     9301 attcatcaat aactggaatc agaaacacac tgataatccc gctacaatta atttttctgg
     9361 tgaggatatt ggtaaacgaa tcctagacct tgctaacgga gagtttgatt tcctagtttt
     9421 tgacaaggta tccgttcaaa agattatcaa ggaccgtggt ttagacctct cagtcgttga
     9481 tttaccttct gcagatagcc ccagcaatta tatcattttc tcaagcgacc aaaaagagtt
     9541 taaagagcaa tttgataaag cactcaaaga actctatcaa gacggaaccc ttgaaaaact
     9601 cagcaatacc tatctaggtg gttcttacct cccagatcaa tctcagttac aataacactc
     9661 ttcgaaaatc tcttcaaacc acgtcagctt tatctgcaac ctcaaagctg tgctttgagc
     9721 aacctacggc tagcttccta gtttgctctt tgattttcat tgagtataag atatactaaa
     9781 agcgattgga ccagccaatc gcttttttag tttgcattgg ctattttagg aaactcttac
     9841 aaaaaatcaa aaaaattctt cacatcctct acatacccat gcttttctat taagctggct
     9901 aaaagttcca gattgtgccc ctgatagtta tcgtcacgac gcagttcttc atacatttca
     9961 ataaagggaa gccccttgga cttggccaat tctaactcag cctcgtaatc ataagcaact
    10021 ttacgaaatt ggatattgac caattcccca tcttcaactt ctatcacggc atactgggca
    10081 cggtgatttt ttaacgcctc ccaattaaaa tagggcatgc caatcgaccc tggattgatg
    10141 atttgttgcc cttgacttcc ataacgaagc aactgcttgt gaacatgacc ataaactgcc
    10201 acgtccgttt ccgcatctag cagttggtca aatttctctg tatcattctc aactagcaag
    10261 tcaccaccat agttcttatt tggcaaatta tgagagatag aaaagcgcag tccagcaatt
    10321 tctttctttt ccagcaaagg caagcttcgt agccagacag tcgttgcagg atccattcgc
    10381 tccatcaaaa actgagtcat tcgcatgagc tggatttcct gtgggtcttc taagccatat
    10441 tgcccatcta aagcctcaag gacacaatca ttccaattgc ctcgaacact tgccgtgata
    10501 ggaagttcct ttaacagagc gaccaagtca tttgcacctg aaccagggag gaaaatgtct
    10561 cccatgagcc aatattcact ggctccttga gttttagcat ctgcaatcac tgcttctagc
    10621 gccgtcgcat tgccatgaat atctgataaa attgcgattt tatggtttat tgtggtttcc
    10681 ttccgtttgg taatctactc tttcttccgc cacttgaagc aagatttctg tttcctgcgg
    10741 gttcaacttt ctttgcacag cctctgctac tgctcttggt actccaactt cgacaatctc
    10801 atccacactg gcttccttga ttttggtcag agacttgaag tgcctcatga gattttgctt
    10861 gcgtttaggt cccagaccat caatcccatc caattgagat gagaaagaat ttttggagcg
    10921 cagttggcgg tggaaggtaa tggcaaagcg gtggacctca tcttgaatgc gttggaggag
    10981 gaaaaattcc tgagaattgc gagacaagtc caccacttcc agcggatctc caaagagcaa
    11041 ttcatgggtt tggtgcttat cattcttttg cagacctgca attggaatat ccaagcccag
    11101 ttcttcttgg atgacttgct tagcaatatt gacttgacct tgccccccat caatcacaat
    11161 caaatctgga ggagtcaaag cctcacgctg tactcgacca tagcgtcttc gaatgacttc
    11221 tctcatacta gcatagtcgt ccggcccaac aacggttttt atcttgtact tacggtaatc
    11281 ttttttactt ggcctgccgt taacaaagac caccatggct gaaacaggac tggtccccat
    11341 gatgttggag ttatcgaagg actcgatgcg aactggagtc ggaatttgga gcaaacgtcc
    11401 tagattttca atagctcctt gggtcttttc gactgatttt tctagcaggt taaatttctg
    11461 ctccagacta acacgtgcat tttttatggc tagattgacc agctgttttt tctctccacg
    11521 ttggggtttg agaatcttgg tatccaccaa agccttgaca gcttcttcgt caatatcctg
    11581 cggaatcagt acctcattgg gaactaggtg agatttttct tgatagaatt gtcccacata
    11641 ggtcaagaaa tcctcatctg gatcattgaa gtaggggaag agattgacat cgcgctcgat
    11701 gagcttgccc tgacgaacaa agaaaacctg cacacacatc cagcccttat ccacatagta
    11761 gccaaagaca tcgcgatttt ggagatcctt agccatgacc cgttgcttgg ttcgaagcgt
    11821 tccaatagcc tgaatcaggt cacggtattc cgccgcacgt tcaaactcca tactttgtgc
    11881 tgctactgcc attttactct tgagatcatc gatgattttg tcatcctgac ctttcagaaa
    11941 atcagacacc tcctgagcca tagacttgaa ataagcctca tccttcttac agatggtgtg
    12001 ggccatacac tggccgatat ggtaataaaa acagacctta gagggcgggt tggtacattt
    12061 tcgaaaaggg aaaatcctat ctagaagtcg cttgatttca ttggctgccc ccacatctgg
    12121 ataagggcca aaataaagac caccgtcctt tttgacctga cgagtaatga taagccgtgg
    12181 atagcgctca ttggtgattt tgatgaaagg ataggacttg tcatccttga gcatgatatt
    12241 gtacttgggc ttattttcct tgatcaagtt gatttccagc agaagcgcct caatattgga
    12301 ctcagtgacg ataaattcaa aatccacaat ttcagacacc agagcctctg tcttggtatc
    12361 atgaattcca cgaaaatagg accgtactcg attacgcaga tttttagcct ttcctacata
    12421 gataatggtg ccatttttat ccttatgaat gtagcaacca gggctggtcg gcaagagctc
    12481 tagttttgat ttaatcaagt tgttcatagt tccattatag caaaaaaagt agggaaagat
    12541 agtaccctac tcactagtct catatatttt tcggaggttt gcaacatcct cttcctggat
    12601 accataaaag gaacctgctg gttgcatgac agacttctca tctgtatggt ccaagccaat
    12661 cgcatgacct aactcatgtt ctgccgtatg gacaaggcgt tcataggagt agccatagta
    12721 tggattggac aaataataat gattcaaccg caccgttacg gacaagaatt gccctgttaa
    12781 gagattagtt tgactttccg cctctcctgc cacaggagtg cctccgtcgt tcatctccgt
    12841 agccgtaata tccgccttac tagactcagt cacgagttca aagttaaaag caccagtttg
    12901 attccagttc tggattgctt ctaaataagc ctcttgaaag cgtgaatcca tctgcggatc
    12961 caggtaaata cgagcagagg cctgtgccca ccttccttca gaatgctggt gactatctgt
    13021 ctggcttaac ttaggcagtt gccctgtttt ctcccattgg tcaatacttt gttgacccat
    13081 ttttactgac cgttctgctt gctttagcgc tccttgaaaa tccccgttca gataccagag
    13141 aagtccgaaa gcaagcacac ataagagcac aactatccaa accagacgcc aaaacaaacg
    13201 ccacacaaaa gaaaagaaag cccctatcaa acgaaaaagc cagcgcatgt ctctccacct
    13261 ttctagcaaa taagaattat tttactaaaa gaagctaaaa gaaatctaaa tactgacttt
    13321 ttcatcttaa cgtcctacta tttttttgag taactggata gctttgtatt ttaaaggcga
    13381 tggatggtaa cggaaagtac ctgctttgcg tacaatatag ccattaaaat tctgtttaaa
    13441 acgcaaaaca ccatcacttc catcaaaaat cccttgaatg cctaggaagt tgtatttagg
    13501 tattccacgt tttatgcttt ccaacataac atatttttga agcagtgcag gggcatagaa
    13561 cttattaaac tcagtgtagg aaccactaaa gagataagtc gtttcctgag gcatataaac
    13621 aaataaactc ccagctaaaa caatatcttc ttctccatat ttttcaatca agtctcgcgc
    13681 ttctgctttt cgaacttcaa acgtttcaaa ttgactagaa tattctctca gttgattttg
    13741 ttttttctca gaatgaggat ttttactcaa atcaagtcgc aacttgtcca agttttcttc
    13801 tagtttactt tgttcacctt gcaatttgct catatactcc gaaaaattca agcttgctat
    13861 gagaaactcc gcttgttctc caaaagtatc ataaaaatgc tcataatatt ctaaactttt
    13921 atcactatat tctctacgtt cagaggtttc ttttgttata ttcttaaaaa tcgatagttc
    13981 ttcacgtttt aactttttca accgaatgcc aaaggtttca gcctttttca ccaagggttt
    14041 accctttttg ctaaaacttt taagcaaact cttttcagtt aattcagtta aatctttata
    14101 gtataaccaa tctggttctc cacctgggta acctgttgtt aagccatcaa attgataacc
    14161 taaatcagtc aaaccttgaa taatactttt tttctcagca tctattggat taccttggct
    14221 atcaaaagtt tgataagttt cataaggttt tacaagcaac tctaatacac cattttgctt
    14281 ggcatattct tttaactctg cataaaaaac tggaagagca tcttgttggg tataaatcgg
    14341 ccccgaattg agttccatat gcagaccacc cagcatgggc aagctataaa ccagagctgc
    14401 aacttgaatt tctccttctt gtttcaaagc aagataaaca attcgagccc ctcttttttc
    14461 tagcaaatcc cccatctgga cagattgcat aaaggaacga gaagaaacct gatcagaata
    14521 agtctgaaac tcttctttcg tgagtgttgt tagtgccata tacttacttt ctatgttttt
    14581 ttcttaatgt tttacggaaa tcaagagcaa gtcttaacag aggatagaga ggatgagtgg
    14641 gcattgtaaa ttcacccaag tattcttcaa tcgttggatt aaatttttcc ttaaaatgat
    14701 aaagtccacc attgagagag ttttcaacac cacctaaatt ttgccagacc atacctcgct
    14761 caaaggcata gcgagccgtt tcataccatg ttaaaattgg tgcattgtaa cgtttaaaat
    14821 catcatccat accagcatat atattgacag aggtagtacc aaattccaaa ctcaaagtag
    14881 ccgctaaagg aactctcgct tgacctacat ctatatattc ctgcaagaag gtcaattcct
    14941 ctaacaaacg ttctttttcc ttcttctgcg cttctacttt tgaagttcga gtcgactcag
    15001 taaaggtctc ttccaaggct ctatttttcg ctaactgttc ttctaactct tgcgaacgtt
    15061 tagaaacatc caaggtggcc aaggtgatat aggccttgtc cttaaaatta tctaacaatt
    15121 ttttataata ggcttcattc ctcaaatgaa tctcttttcg cttctcagtt tttttcatca
    15181 actccgaaaa tgaatctaat agttccagtc caccatattg aatctcaagc cctttgtttc
    15241 gtgctgttcg aatagcctgt tttgttgact tggaaagttt atcttcttca aaattttcct
    15301 tgtatatttt cgcctgaata cgaggttgaa tggtgtctcc catttcctcc gtttttcctg
    15361 accaccttac tcccatttgt tgcaaactat caataatagc cagattttca ggaaattctg
    15421 tcttttcctg attgattaaa ctttgagata ggcaaatact tgggtcaaaa gtcacaaaaa
    15481 ccgctctctt actgcgagca taggacttaa tagactgaat ggcaaaattc aagagttctt
    15541 tatccccata atccaatata ggtcctcttg ggatgtaaaa cattttatag cctagcggaa
    15601 gagttctaat caaaatacta gctgtcgcca gtaatttttc ttccctgtaa acaccaaact
    15661 tctcatgttg ccaattagac ttaacttcct cccaagcact actttgtaat acattggcta
    15721 attcatgttc tttgacaaac tgatcatatt ctaatgtggg aatgccaatt tgataacggt
    15781 acatttccta ctctctttcc tccagtattt tattactatt ctactacttt cgtttgaaaa
    15841 gtctagtgaa aacatatttt attttagttc tactccccct atgctgaagt tcaaactaag
    15901 tcagtctctc ttctaatttg aaatcaattg gtagggttgt taaaaaaact tccaattgtt
    15961 tttcccagta gtaccactcg tgagttccag cgctatggct ataggtcaca tcaaaaccta
    16021 gttttttgag atttttcact gcgagattat tggcttcgta caagaaatcc tgttcgccac
    16081 accacgccca aagtttggtc tttttatccg attttttagc cagactttca agagaatagg
    16141 gactagttgt ccagtctcta atctctccaa aaacacctct ccagtaggct ggacttccca
    16201 gattttgact ttcaggagaa aagttttgaa agctgagggc acctgaaaaa ctagctgcat
    16261 gagaaaaacg atttgtcgca agagccagtt tgaagcagcc gtaacctccc atagaaagac
    16321 cagcgataaa ggtcttttca cgcttgctag tcatattagg gaagaagcgt ttcagaacct
    16381 gtggcaattc ctctgctaga gccgtgtagt agtcaaaacc atactgggta tcggtgtacc
    16441 aaccattgct ggtattgggc ataacaacga tgagattagt tcctcgaagc aagcgttcta
    16501 cattggtccg cttaagccaa ctattatgat ttccagacat cccgtgcaaa aggtacaaga
    16561 cgggaatatc ttcacactct ggttcttcca ctcgattggc atcagggtag aggacattca
    16621 ccccccactc catatccaat acttgtgagt aatactcgat tttcattact gccatgattt
    16681 tctccttcta tttttctata agaaaaagcc gaaactcgac tttctctctg tttatttcaa
    16741 cgattatttt gcttccatga ctactggtaa aatagctgga cgacgcttgg tttggtcaaa
    16801 gagatacttg gtcaaattat ctcgaacctt ccctttaaga tctgcccagt caaagtcatc
    16861 tccttgaaga taatcttcta ccgtttggtt aatcaattct gaactttcac ggagaatatc
    16921 gcgactcttc ttgagataaa caaatccgcg cgtatgaaca cgagccctag ccacaatttt
    16981 cttctcacga cggttgactg taatagccac gatgaaaatt ccatcctctg acaagatctt
    17041 acggtcacga agaacaacat ttccaacatc accaatggca ttcccatcaa tcaagatatc
    17101 tcctgctgaa accgatccag ctggaacaaa gtctccattc tcgtaagcca tggtcgtccc
    17161 ctttttagga atgaagatgt gttctggcaa catcccaact gccatggcag ccttagcgtg
    17221 agcatccaac tcacgatact ccccttggac agggaagagg tacttaggtt gcaaaagatt
    17281 gatcatcagc tgcaaatcac gcacatttcc gtgccctgat acatgtaaac tttgggtaat
    17341 caatttgaca accccacctg cctgataaat catattttcc acacgcgcaa caaaggcttc
    17401 tttagcaata gacggagccg tagcaatata gaccaggtcc ccatccttga tttctacata
    17461 acgatggcga ccaatcgaca tcttacgaag tccattgata ggttcaccca tacgacctgt
    17521 ctcaagaata atcaactcat ggtcttcaaa gcgagacata tctttaggct taatcaagag
    17581 aatttcgttg gctaaagaca acttcttaag acgaatcgct gtgcggacga tattttcaat
    17641 atcaaatcct gtcaagacga tacgtcgacc tgttttatcc gcagcgtcaa aaatctgctg
    17701 aatacgagaa agattactgg aaacagctgc aacgatgata cgaccttccc agtcagcaat
    17761 agtttgggta atttcatccc taacttcact ttcactagcc acctgaatat tgctgtctgc
    17821 attggccgaa tcactgagga gagccaggac gccgtcacga ccaatctctg ccaaacgagc
    17881 aaaatcagtt gcataagatt cactagccgt ttggtcaaat ttgaagtcac ctgtataaac
    17941 gatgcttcct tccgatgtct tcaagacaat tcccagactc tctggaacgg agtaagtcgt
    18001 agggaagaag gaaaccactg tcccaccaaa atcaatctcc gtattctcat caatgacatg
    18061 gaaatcatta aatttcttaa cggcatcatt tcctttgaca aagagctttg ccaactcaat
    18121 ggtcaactca gacccaaata caggaacttt agcctctgcc aagagataag gtagagcacc
    18181 aatggcatcc gcatgcccgt gggtcaagaa aaccccagca atacggtcgc tattttcaaa
    18241 aaggtaatcc atgtttggaa tcaccacatc gacccctaat tgttcatttt caggatattt
    18301 taaccctaca ttcaaaacaa aaatggactc tccaatttca gcaatgtaca tattttttcc
    18361 attctcacgc acaccaccaa gtgttgttaa actgatatta ctcatttttc ctccaaaatc
    18421 ttagtttatc ttgattatag ggcaattcta ccctcttatt ctaaaagttc tgatactttc
    18481 agccgaatct tttcctacca ttataccatt tttttgatga ttttcaaaat gaaggtctgt
    18541 tttcaaaaaa gagttgattt cccaactctt tcctaacttc tactcttttt ccatcaaaaa
    18601 atcataaatt tcttgcaccg tacccagcgc catcatttct tggtctttaa cggtttgttt
    18661 gagattttgg taaatttgac tttctcctat ctcccgcttt ggcgcctctt tattgactgt
    18721 catcacacca tcttttatcg tcacaaagcc tagttcttca aatacttgaa tcatcttgac
    18781 cagcaagatt tgttgaatat taagatatgc agccaaatct ttcagcttgt agcgaatatc
    18841 aaactctggg aactggtaaa tagtcttgta caatttagca aactgatctc tagtcccata
    18901 acctgtcaga taataggtct tgtcaatatc atttttgaaa tagacagcag agaaatgctg
    18961 ttcctgaaaa atggtcttca gctgagtaat atcctctgga atgtttttta cgacaacagc
    19021 ttcactagcc gcaagatttg gcagttctcc aggaaaatcc aagactggaa caccttctgg
    19081 caagactgca ttttttccac gaatgttaaa aagttgaaca ccttccactc gcgcatccac
    19141 catcatcaac tggagggcag tttggccatt ccattggttg acagacaatt taaccgctaa
    19201 ctctagattc ttggtttgag aaaactctgt cgcccatctg ccttgaccaa aggctaccac
    19261 ttcaaaactc gcctcaccct tggaaatttt cagctttaga tgggcattac ctgcccccat
    19321 agtacgagca ctttcgacct gaaaattctt gatataaaaa ataggtttct gattatccat
    19381 tccaaaagga gctaaacgtt caaaactttt gaccgtttcc aagctaagtg cctccaaatc
    19441 caactcttca tctaggttta acttattctt gccaccagca tctgcacctt tttcacgaac
    19501 ataatcttcc aaaacctgag ataaatctga gagttgctca acttccagcg tcatacccgc
    19561 tgcacctgca tgacctccaa aggcgatgaa gaggtctcga tggggatcca gagcttcaaa
    19621 aatatcgacc gcttccacac tacgagcact gcccttggca cgaccgtctt ctatattaag
    19681 aacaatgact gtctgtccca attcttccaa taaacgacca gccacgattc ctagaacccc
    19741 aggattccag ccttccttgg ccaagacctg aaccttcttc tcaggatcca ccatggtctt
    19801 ggcttcttca tagatagact gaacgatttc cttgcgctct tcgtttttct ggtgaatcat
    19861 aagggcaatc tcatgcgctt cctcatcatc aaatccagtc aacaaatcaa tggcaggatt
    19921 gggatcatcc aagcggccca aggcattcaa acgaggagca atctggaaac caaccgtttc
    19981 ttctgttact tcgttggcag caatcccagc catgtccagc atttcttgca gaccaatgcg
    20041 ctgggtatga cccaacattt ccagaccata ttgaactaag atacgatttt catccgtcag
    20101 actcaccata tctgcaatag ttccaatagc gaccaaatca agcaattcca cttgcacttc
    20161 ttctaacagg gcacaagcca acttgaaagc aactccacaa ccagccaaat atttaaaagg
    20221 ataatccgca tctggatgtt caggatggac aatagcataa gcatctggca gggtttcagg
    20281 catggaatga tggtctgtca caatgacatc tactcccata gactgagcca atgcaatagc
    20341 ctcatgacca gcaaccccat tgtccaccgt cacaatcaag gaaattcctt cttgctcgat
    20401 aaagtattta taaacactag cattagggcc atagccatcg gtaaaacgat ttggcaggta
    20461 aactcggcac tcagcaccaa gttgttccaa actttccttc acaatagaag ccgaagtcat
    20521 gccatccgca tcatagtctc cataaacgag aatattttcc ccttcttcaa tagcctgacg
    20581 aatccgctcc actgccttgt ccatatcatg gagcagataa gcatcatgta agtcctccaa
    20641 ggaaggttct aaaaacttct tcagactttc ttggtcctga atccctctct caaacaataa
    20701 ccgagccacc tcaggaccca gtccagcctt cttggctatc tttgtaaaat ccgcatcttc
    20761 tacctgcagg gcaaactgcc attcataagt aggtgttatc aaaaagacat ccactctttc
    20821 taagaattct attgcttcat tataacatga tttagacgtt ttctctatcc agattgcttt
    20881 tttataacat tttatagtat attgaaacta gaatagtaca cctctacttc taaaatattg
    20941 ttagaaatcg atttgactgt cccgatcgat ttgtcctgtt cttatttcac tatagtgaat
    21001 tttttcagat atggtttgac aagaacattc tttcattgta gaatcgtgtt ataaaatata
    21061 acacaaagga gatctctcat ggctttagta gaatttaaaa acgtcgaaaa atattacgga
    21121 gactaccacg cactccgcaa catcaatctc cgttttgaaa aaggacaagt tgttgtcctg
    21181 cttggacctt ctggctctgg gaagtccact cttatccgta cgatcaatgg tttagaggct
    21241 gttgacaaag gaagtctcct agtcaatggg caccaagttg ctggtgccag ccagaaagat
    21301 ttggtacctc ttcgcaagga agtcggcatg gtttttcaac attttaacct ttatccacac
    21361 aaaacggtgt tagaaaacgt gacacttgcg cccattaaag ttctaggaat tgataaaaaa
    21421 gaagctgaaa aaaccgccca aaaatatctg gaatttgtaa atatgtgaga caagaaagat
    21481 tcctatcccg ccatgctatc tggtggacaa aaacagcgga tcgccatcgc tcgtggtctt
    21541 gctatgcatc cggaactcct cctctttgat gaaccaacat ctgctcttga tcctgagact
    21601 atcggagatg ttctagcagt tatgcagaaa ctggcgcatg atgggatgaa catgatcatc
    21661 gttacccacg aaatgggctt tgctcgagag gttgcggacc gcattatctt tatggccgac
    21721 ggagaagttt tagtagatac gacagatgtc gataactttt ttgacaatcc aagcgaacct
    21781 cgtgcccaac aattcctcag caaaattatc aaccacgaaa gtgacaaagt caaataagga
    21841 ggcgcctatg aaaaaaaatt ctttttatca gcattattga ttagcctttt cggccttgct
    21901 gctgccaaac cagtccaggc tgatactagt atcgcagaca ttcaaaaaag aggcgaactg
    21961 gttgtcggtg tcaaacaaga cgttcccaat tttggttaca aagatcccaa gaccggtact
    22021 tattctggta tcgaaaccga cttggccaag atggtagctg atgaactcaa ggtcaagatt
    22081 cgctatgtgc cggttacagc acaaacccgc ggcccccttc tagacaatga acaggtcgat
    22141 atggatatcg cgacctttac catcacggac gaacgcaaaa aactctacaa ctttaccagt
    22201 ccctactaca cagacgcttc tggatttttg gtcaataaat ctgccaaaat caaaaagatt
    22261 gaggacctaa acggcaaaac catcggagtc gcccaaggtt ctatcaccca acgcctaatt
    22321 actgaactgg gtaaaaagaa aggtctgaag tttaaattcg tcgaacttgg ttcctaccca
    22381 gaattgatta cttcccttca cgctcatcgt atcgatgcct tttccgttga ccgctctatt
    22441 ctgtctggct acactagtaa acgcacagaa ctactagatg atagtttcaa gccatctgac
    22501 tacggtattg ttaccaagaa atcaaataca gagctcaacg actatcttga taacttggtt
    22561 actaaatgga gcaaggatgg tagtttgcag aaactttatg accgttacaa gctcaaacca
    22621 tctagccata ctgcagatta aggaggacac cccatgatag atttatcatc ttggacagcc
    22681 tattttcagg attttggaca atttttcaat ggtttcctct ttactcttgc cctagcgatt
    22741 ggatccttta tcctcgccat ggtcttagga atcttctttg gtgccatgtc aaccagtaaa
    22801 cgtccaattt tacgcatttt agctcacatc tttgtggaat tttaccaaaa cactcccctc
    22861 ttggtgcagt ttgttattgt tttttatggc ctacctctta tcagtgacca tactatcatg
    22921 attccaattt attggacagc tgttctctgc atgggactct atcacggcgc ttatatcgct
    22981 gaggttattc gttcagggat tcagtctatt cctagtggtc agatggaggc cgccttgtcg
    23041 caaggtttta cctatatcag tgccatgcgc ttgattatct tgcctcagac cttccgcatc
    23101 attctccctc cattgaccaa ccaaattgtt aacctcatca agaacacctc tactgtagct
    23161 atcatctctg gagtagactt gatgtttgtg actaagtctt ggtcggctct caacggaaac
    23221 tatatcccag cctttttagg tgctgctctt ctctactttg ctctatgctt ccctgttgcc
    23281 cagtttggtc gcaagatgga gcaagccaac aaaaaagcct attcacttta ggaggttact
    23341 atggaatcta ttttagaagt tttaacccca gataacctag tctttatctt taaaggattt
    23401 ggcttgaccc tctatatttc tctgattgcc atcatcctct ctactatcat cggtacggtg
    23461 ctagctgtca tgagaaatgg caaaaatcct gtcttacgca ttatttccag tatttatatc
    23521 gagtttgtgc gcaacgttcc caaccttctc tggattttta ctatcttttt ggtgttcaaa
    23581 atgaaatcca caccagcagg tattacagcc tttactctct ttacatcagc agccttggct
    23641 gagattattc gaggcggtct caataccgta gacaagggac agtacgaagc aggaatgtca
    23701 caaggcttca cctcagccca aatcctctac tacatcattc tcccacaagc catccgcaaa
    23761 atgctaccag ccatcatttc tcagtttgtt accgtgatta aggataccag tctcctctac
    23821 tctgttatcg ccctacaaga actctttgga gccagccaaa ttctcatggg ccgttatttc
    23881 gaaccagagc aggtcttcag tctttgcatc ctgattgccc tcatctactt cagctttaac
    23941 ctagcaattt ctagcctgtc tcatatgcta gccaaacgtt ggcaacaagc tgcagaataa
    24001 tactcttcga aaatctcttc aaaccacgtc agcttcacct tgccgtatat atgttactga
    24061 cttcgtcagt tctatccaca acctcaaaac ggtgttttga gcagcctgcg gctagcttcc
    24121 tagtttgctc tttgattttc attaagtata aacagccact ctccagttca attggagagt
    24181 tttttgatga gagtagatat ttatagacct caatttgaca ccatacattt tctatgataa
    24241 aatgtaacaa attcatttac aacaaatcga aatctaaatg aaatggaatt ttctatgaaa
    24301 tcagtcaaag gactactctt tatcatagct agttttatct tgactctttt gacttggatg
    24361 aacacttctc cccaattcat gattccagga ctagctttaa caagcctatc tctgactttt
    24421 atcctagcca ctcgtctccc actactagaa agctggtttc acggtttgga gaaggtctac
    24481 accgtccaca aattcacagc ctttctctcc atcatcctac taatctttca taactttagt
    24541 atgggcggtt tgtggggctc tcgcttagct gctcagtttg gtaatcttgc catctatatc
    24601 tttgccagca tcatccttgt cgcctattta ggcaaataca tccaatacga agcttggcga
    24661 tggattcacc gcctggttta cctagcctat attttaggac tctttcacat ctacatgata
    24721 atgggcaatc gtctccttac atttaatctt ctaagttttc ttgttggtag ctatgccctt
    24781 ttaggcttac tagctggttt ttatatcatt tttctatatc aaaagatttc cttcccctat
    24841 ctaggaaaaa ttacccatct caaacgctta aatcacgata ctagagaaat tcaaatccat
    24901 cttagcagac ctttcaacta tcaatcagga caatttgcct ttctaaagat tttccaagaa
    24961 ggctttgaaa gtgctccgca tcccttttct atctcaggag gtcatggtca aactctttac
    25021 tttactgtta aaaattcagg cgaccatacc aataatatct atgataatct tcaagccggc
    25081 agcaaagtaa ccctagacag agcttacgga cacatgatca tagaagaagg acgagaaaat
    25141 caggtttgga ttgctggagg tattgggatc acccccttca tctcttacat ccgtgaacat
    25201 cctattttag ataaacaggt tcacttctac tatagcttcc gtggagatga aaatgcagtc
    25261 tacctagatt tactccgtaa ctatgctcag aaaaatccta attttgaact ccatctaatc
    25321 gacagtacga aagacggcta tcttaatttt gaacaaaaag aagtgcccga acatgcaacc
    25381 gtctatatgt gtggtcctat ttctatgatg aaggcacttg ccaaacagat taagaaacaa
    25441 aatccaaaaa cagagcttat ttacgaagga ttcaagttca aataagtaat catactctcc
    25501 tgggatttcc caggagagtt ttttatagcc aaatgaaata aaaccggaac gatggaattt
    25561 tggcattcaa atcaattgat aagaatattt tatagtggag tgaaatagga tacgaacaaa
    25621 ttgattagga aaatcaaatg aatttataga aatcttttag cagttatgtt gtcctattct
    25681 agtttcaaaa cgctatagaa gcagcattgt gctagtctag attcagttta ctatactaaa
    25741 acgagtagct tgaaatcaaa aaacccaccc tcacaggcag gttttatctg tattattcag
    25801 ctagattatg ctttaccttc tgaaccgaat acgtcgatac gttcttcaac cgatgcttgg
    25861 atagctttta caccgtcagc caagaattta cgtgggtcga agagtttttt cttgtcgtat
    25921 tctgcttcgt ttgcttcgta gtcacgagca aatttacgag ttgcgttagc gaatgcgatt
    25981 tggcattctg tgttaacgtt aactttggca acaccaagtt tgatagctgc ttggatttgc
    26041 tcatcaggaa tacctgatcc accgtgcaat acgattggga atcctggaag agcttctgtc
    26101 aatttttgca agtggtcaag gtcaagacct tcccagttta ctgggtaagg gccgtggatg
    26161 ttaccgatac cagctgccaa gaagtcgata ccagtttcaa ccattgcttt agcgtcttcg
    26221 attggagcca attcaccttt accgatgatt ccgtcttctt caccaccgat agtaccaact
    26281 tcagcttcta ctgagatacc tttagcgtgt gctttttcaa caacttcttt agccaattta
    26341 aggttttctt caactggaag gtgtgaaccg tcaaacatga ttgaagtata accaacttcg
    26401 atacactcaa gtgcatcttc gtagtgaccg tggtcaaggt ggatagctac tggtacagtg
    26461 atacccattg attcaacaag gttagcgatc aagttgcgag caactttgta accacccatg
    26521 tatttagcag cacccattga agtttggatc aaaactggag cttttttagc ttctgctgcg
    26581 cgcaagatag cttgagtcca ctcaaggttg tttgtgttaa atccaccaac tgcataaccg
    26641 ttgtcacggg ctgcttggac aaatttttct gctgaaacga ttgccatttt atcaggcctc
    26701 ctgtatattt ttatgggtca tcccatttac attgttcatt ttatcacttt ttgccaaaaa
    26761 aatctagttt ttcccgcagt ttcgattgat tttcttctaa ctccatctat gtaaaccctt
    26821 tctctcccta gtcttggacg acttttggaa aatctataaa gaaggttaaa cgattctcct
    26881 ccatctcgaa acgataagct aatttttcat gttctaatag actcttaacc acaaagagcc
    26941 ccataccaga ccccttgacc ttgcgactgg cattgtcaga aaaagactgg gctagttttt
    27001 cttgttcctc tgagctacag ctattttcga taaaaagttc tccttctctt tctccaattc
    27061 gaactaagcc acctggaaca gagtgcttaa tggcattgct gatgagatta gaaagaatca
    27121 acttcataac tgatgggttt agataagcct gctgatgggt caaactattg tctatctgga
    27181 gctctctttc cttggctagc aaggcataat ctttgaccag attttgcgtc atctggagga
    27241 ggtcaattgt ttccctatca tctcgcaatt cctgcacaga agagagggaa agtatctgca
    27301 gaacatggtg attgagttca tccacaatcc ccaaggcaac tcccagatac tggtctctat
    27361 ccttataacg accgatattc tctctcatat tttcgattag gattttcaaa ctagccagcg
    27421 gtgttttcaa ttcatgagaa gctcctcgta ggaattcgac cttcatcttc tccagctgga
    27481 gaatggcttc attcttttca tgcaagtccg caataacagt caagagatgc tggtagaggc
    27541 tattgatttg ttccttgaga ttacctatct catccttaga atccacgcgc aatcgcactt
    27601 gggaatccag gtccatcatc cgacgggtca cccgcttgat ttccaagatc ggtgcaacaa
    27661 tagtccgagc gtagatgtag gccaccaaga gggaaatcag aaaggaggcc agcaaggtat
    27721 agggaagaaa ctggagactg atttgctccg cttccttttg taaatccatg gaagctagaa
    27781 actggagaat catagtagca ccgtcttgcg ttttcacctc gcgctcctca ataaagagag
    27841 aggttgtctg gcggtctgtg tccagaggaa gactgtcctt gacttctaac ttgtcctcgg
    27901 tcatctcacc tttgacggtc cccttgatat cactagtctg ggaatacaag tctaacactt
    27961 gctcgatact ctgcctatct ttcccttcta gggactgggc aatggctgtt gccttttgac
    28021 caatggtttc ctgacgatga ctcagataag tcgaaggaaa aagaaaataa atagctaaat
    28081 gaaggcagat aaccagaaca ctaaatatcg agaaggtata gataaatatc tttgcaaata
    28141 aacctgttcg tttcattttc gctccaattt ataaccaaca ttgcgcacag tgaggataca
    28201 atccaagtct agctttttcc gcaattcctt gatataaaca tcaataacac ggtcaaaggg
    28261 aacctcatct gtcgctttcc agacggcatc gataatctga gatcgagtca aggcccggcc
    28321 ttcatttttc actagatagt ccagaatttc caactctttg gcattgatag gcacttcttg
    28381 acctgcgatg cttgcactgt agctttcaaa gtccaccttg gtatccttgt aagaaaagat
    28441 tcgtcctgta tcgtagtagc gcttgaaaat cgcgtccacc ctcactttta aaagggagag
    28501 ggagaaaggt ttttccagat agccatctgc caaagaggca aaggcactca tcttgtattc
    28561 ctcatcttga aaagctgtca acatcaagac aggaacctga ctggttttac gaatctcagc
    28621 taggacttct aagccgttga gcttgggcat ctggatatcc agtaaaacca gggccacctc
    28681 atagctagaa aattgctcca gagcttcctg accgtccgct gcctcaatag tttcatagcc
    28741 acaatccgtc aaataatcac tgaccccctc acggatcatc tcttcatctt ctacaattaa
    28801 aattttcata ctttaactgc tctctatttt ttatttttct tagaataaat acctactcta
    28861 ttttctatta tagtctcttg ctggcctttt gtctgcaagc aactgaccac tagataaaac
    28921 gttgtgaaat tcctttctca taaattccat aactttagta tattatattt aagcactaaa
    28981 gtacaaagaa agcaactgaa agcaatgatt ttcaccactg ctttcggatt tattttgaat
    29041 tgttaaatag ccattcctat ccactattct tgaatagaaa cacaagatgc aatctttatt
    29101 ctagactcat tttttcaaat ttattcacca tccagcaaga gctcttttgg ttgttttcta
    29161 aggagattgc ttgaagcaag cgccataacg agaaccacta gaaccaaggc aaggacaaaa
    29221 atgatgataa agtctgatgt ctgaatggaa atgtctaggc tcgacaaggt cttgctaaag
    29281 ccatctactt ctgcaccacc accaaggtta gaggcttgag ccgccttact agcctgtttg
    29341 gcaacacctg aagtcacatt ggcaaggaca gtgtttccaa ttgcacgggc agtgtaatta
    29401 gctaggaagt aagcagaaac tagagcaggg atagcaatca agatagattc ggtgatgaat
    29461 tgacccaaga tacttgcctg cttgaggccg atagagagga gaattcccac ttccttgcga
    29521 cgggcgttga tccaaaggct gagcaagagg gcaaggagga gaactgagaa gctcaagcta
    29581 ccccagaaga ggaggttggc catcttgtac ataccagaga tagattgctc aagagctggg
    29641 tagttagagg agctcttgac gagtgtgtag ctcttccagt tgataccact gatgccattc
    29701 aactctttca taacatcatc caagttcttg tctgctgtta caaagaaggt tgcgtcccca
    29761 taaatggctg tgtcttctgt gtatccataa agttttgcag cagtgtgaat gtctgtaata
    29821 gctgtgtttt cgtaaagttc ttgtgagtag gttactgctg acttattatg accatcaaag
    29881 agtcccttga ttgtcacttc aactgtttcc ttggctcctt tttcattatc tgcatcgtag
    29941 atattagagt ccagtttaac cttgtcccct actttccagc cgtgtttggc tgccaagtcc
    30001 ttgtgcaaga ggattttatc cttgtcgtcg ttggttaagt gctctccttc gactagttta
    30061 taagaaccag agacaaactt gtcttcttta gaggagtcat tgacacctgt aatcatcaag
    30121 ctacttccaa aacgcttggc acgatcagca gtgagattct tcttggtttc tggcgtttca
    30181 atcaggtcat atccagtcaa atctccgata gcgttgatac gtttgacata agactcgatg
    30241 gccttgtttt cggtgatttt tttgatgtct tcacccttga tattcccagc accacgaggc
    30301 gttccttggt tgacgcgacg attgatttgc atggagaagc tattagtgat atttttaaag
    30361 gtctcctgag aagccttggc agtagctccc ttgattgaca agccgaccaa actcaagctc
    30421 gccatgagga gaataatcag gaagatgaca atcgatttga aaaacttcct tgtaacatag
    30481 gcaaatgcgt tgtgtaacat agattccctt tctagatttt gttttaatca ttctatcaaa
    30541 ataagctcaa attatttact agtattgcgc gtttcagtca gtttcttatc ctttaattca
    30601 agtgtaatat ctgacgcttg tgccacttct ttactgtgag ttacgacaat cacacattta
    30661 cctgttttct gggcaagtga tttgagtagt tcgacaatat ctccagcagt tttaggatcc
    30721 agatttcctg ttggctcatc agctagaata actggagctt ctgagaccaa actgcgagca
    30781 atggcaacac gttgctgttg accacctgat aactggagaa cattccgctt gatctggctt
    30841 tcatccaaac caagctcaag aagtgtatcc ttgcttgcct ttttgttgac caagcggata
    30901 ttttccagcg gagaaagata atctatcaag ttataatttt gaaagaccag ggaaatatgg
    30961 tgcatgcgat ggtaagaata gcccttctta cgaatatcct ctccttgaaa aaggatagaa
    31021 ccttcaacag gactatctag accagcaagt agggacaaga gtgtggattt tcctgctcct
    31081 gactccccaa taatactgta aaattttcca ggttcaaaat tataatcgat ctgatatagg
    31141 actgcttcag cagtattctt ataacggtag gtaacatctt gtaattgtaa taaagtcatg
    31201 atttctcctt cttaactaat agatgataaa atttctttcg gtgattttct aaataagaat
    31261 aggaaacaaa gggctacaga taagcaacta agcagaacta gaaaaacata ggattctgca
    31321 aaagataaga tgctagttga taaactgctt gctttggcta gtgtatcttg taagcttgcc
    31381 tgatctccac ttgctagtag agtttggagt agataacttg tgattccgtt tcctgcaaca
    31441 aatgctggaa gcaaagctcc aagagatacc aaaactacct ctaaacagaa ttgtaggaag
    31501 atcgagctct tgccttttcc aagtgcaagt aaaatcccca cttcatagac ccgttctctc
    31561 aaccagagag acaaaaccag aattaaggct ccagctcctg ctatcaacat cccataaagg
    31621 aagatggtca agaaggtttg gaaagttgca actgagtctt tgatttgttc aaaagccttg
    31681 ttttcctttt cgacttggta gccttgattt tccaaggcca agttttctac ctgcttcatg
    31741 agtccgtcca tttccttagg attttctaca tagaagcgtg ctgcactgac ttgagcttca
    31801 ctattgccca aaagggtttg gctactttca tagtctgtaa agacttgatt ttcactgaag
    31861 tcagaagaca agcctgtgaa tttctcttgt tttttaccag aaaagatgcc gataatctca
    31921 aactctactg tttgtccttt tccagattca gactgaccag catccaagcc aatcttgtca
    31981 tgaagtgaaa gaccgttctt cttagccaat tcttcgtgga taaggatttt cttggaatcc
    32041 cctttttgaa ggtgtcgccc ttcttttaga ttgaaagccg aactggtaaa ggttacatcc
    32101 ttggatgaat cctcaagagc cgttaagcta accaagttat tgtctgcagc tgataaatca
    32161 tcacgctcca cgctctgctc accagtcact gcttccttgt cttttagttt tgcgaccgtc
    32221 tcaagttcag gagagacatt ttccagcccc ttaatcttgc ttacagatgc taggtctgac
    32281 aacttgaatg tctgaccatt ctctatcttc ttaatagaaa aagatgtatt gagtgattta
    32341 taaagattgc tttctactgt tttgttggac ttcatcagag tcaaacaggc tgaaattccg
    32401 gccaataaga ccaataaaat cagaaataaa ataaaacttc tcagtcgctt tctgctgaca
    32461 taagcccaag atctttggat tggattcatt tgtcacctcc atatttgtaa gactattata
    32521 aaacccaaat atgaaatgtt tatgaaatac gaaaaaaaat atcgagtagg ggataatctc
    32581 tagcccctct cacaccaccg tacgtgccgt tcggcatacg gcggttcaac taacttttaa
    32641 cgcatgtcgt tcaaggtaat aatccaaaca cgaaacctgt tcacgttttt caaggactgg
    32701 ttttgatata gcacgtttaa gtaccgactt ctgagctact atagtagatt gaaactagaa
    32761 tagtacatct cttcttctaa aacattgtta gaaatcgatt tggctgccct gatcaattcg
    32821 tccta
//
LOCUS       DAVBBS010000019        31002 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVBBS010000019 DAVBBS010000000
VERSION     DAVBBS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31002
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.019"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(298..516)
                     /locus_tag="VQU32_000863"
     CDS             complement(298..516)
                     /locus_tag="VQU32_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2472340.1"
                     /translation="MVLNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            complement(762..1040)
                     /locus_tag="VQU32_000864"
     CDS             complement(762..1040)
                     /locus_tag="VQU32_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472341.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEMLELACWFRVIIVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAAYSIHFNNGYCKQHTNEFKDSLKSLIL"
     gene            complement(1254..2885)
                     /gene="rlmD"
                     /locus_tag="VQU32_000865"
     CDS             complement(1254..2885)
                     /gene="rlmD"
                     /locus_tag="VQU32_000865"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET2472342.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSLHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWCTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSQKNASLNKITNAHYVCDTAENAMKKWLKEGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2959..3933)
                     /locus_tag="VQU32_000866"
     CDS             complement(2959..3933)
                     /locus_tag="VQU32_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HET2472343.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(4007..4621)
                     /gene="pmp23"
                     /locus_tag="VQU32_000867"
     CDS             complement(4007..4621)
                     /gene="pmp23"
                     /locus_tag="VQU32_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HET2472344.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(4621..5595)
                     /locus_tag="VQU32_000868"
     CDS             complement(4621..5595)
                     /locus_tag="VQU32_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HET2472345.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQKENGTYSILIKNIEDIQSK"
     gene            complement(5603..6859)
                     /locus_tag="VQU32_000869"
     CDS             complement(5603..6859)
                     /locus_tag="VQU32_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET2472346.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(6921..7352)
                     /locus_tag="VQU32_000870"
     CDS             complement(6921..7352)
                     /locus_tag="VQU32_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2472347.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(7354..7956)
                     /locus_tag="VQU32_000871"
     CDS             complement(7354..7956)
                     /locus_tag="VQU32_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HET2472348.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7940..8779)
                     /gene="prmC"
                     /locus_tag="VQU32_000872"
     CDS             complement(7940..8779)
                     /gene="prmC"
                     /locus_tag="VQU32_000872"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET2472349.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(8779..9858)
                     /gene="prfA"
                     /locus_tag="VQU32_000873"
     CDS             complement(8779..9858)
                     /gene="prfA"
                     /locus_tag="VQU32_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET2472350.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9855..10445)
                     /locus_tag="VQU32_000874"
     CDS             complement(9855..10445)
                     /locus_tag="VQU32_000874"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HET2472351.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRGLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            10568..10750
                     /locus_tag="VQU32_000875"
     CDS             10568..10750
                     /locus_tag="VQU32_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HET2472352.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10887..12260
                     /gene="mnmE"
                     /locus_tag="VQU32_000876"
     CDS             10887..12260
                     /gene="mnmE"
                     /locus_tag="VQU32_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET2472353.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEANLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLIEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(12307..13242)
                     /gene="dapA"
                     /locus_tag="VQU32_000877"
     CDS             complement(12307..13242)
                     /gene="dapA"
                     /locus_tag="VQU32_000877"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET2472354.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(13298..14374)
                     /locus_tag="VQU32_000878"
     CDS             complement(13298..14374)
                     /locus_tag="VQU32_000878"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET2472355.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPCDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14716..14788)
                     /locus_tag="VQU32_000879"
     tRNA            complement(14716..14788)
                     /locus_tag="VQU32_000879"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14753..14755),aa:Thr,seq:ggt)
     gene            complement(14888..15397)
                     /locus_tag="VQU32_000880"
     CDS             complement(14888..15397)
                     /locus_tag="VQU32_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HET2472356.1"
                     /translation="MKKLTIRDIADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(15395..15491)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            15520..15951
                     /locus_tag="VQU32_000881"
     CDS             15520..15951
                     /locus_tag="VQU32_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET2472357.1"
                     /translation="MKIPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKAYIFRK"
     gene            16033..16410
                     /gene="mscL"
                     /locus_tag="VQU32_000882"
     CDS             16033..16410
                     /gene="mscL"
                     /locus_tag="VQU32_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET2472358.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(16452..17546)
                     /gene="hemH"
                     /locus_tag="VQU32_000883"
     CDS             complement(16452..17546)
                     /gene="hemH"
                     /locus_tag="VQU32_000883"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET2472359.1"
                     /translation="MKKAILMMTFGSPEEISFEGVAEFFTNIRRGVRPQDHEIQTLYD
                     NYIRIGGTPLQRITREEVNLVKERLGEEYGIYFANKFSRPFIPDVIKQMETDGVEECI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEVLLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            complement(17624..18456)
                     /gene="srtG2"
                     /locus_tag="VQU32_000884"
                     /pseudo
     CDS             complement(17624..18456)
                     /gene="srtG2"
                     /locus_tag="VQU32_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218039.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG2"
     gene            complement(18534..19532)
                     /gene="srtG1"
                     /locus_tag="VQU32_000885"
     CDS             complement(18534..19532)
                     /gene="srtG1"
                     /locus_tag="VQU32_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG1"
                     /protein_id="HET2472360.1"
                     /translation="MKTKREKPKKSLSRRLVLAVDGVINHLLLIFAALIFLFGFYALW
                     DSNQVYSLASSSEYEAYRPVTTQQDELASFSGFSKLQELNPEVLGWINVYGTNIDYPL
                     VQAKDNEKYLNKDSKGEFAATGAIFLDARNNPKFEDFNTIIYGHHVENGVMFGDVAKF
                     ADQEFFDQHRYGSIYYNGVEKGLEIFEMLEVDAYDFNIYDPGIQGEDRQQAYLDHLLS
                     VAMHKRDISLSPSDRIILLSTCFLDVTNGRHIVVAKITDTVPKNTFHTKKSKPFPYSV
                     FDDSSLGRFLSSIPLWIWYLILFVLFLLLIFLLLVLYLILRRRRESKKNARSRPFY"
     gene            complement(19550..20746)
                     /gene="pitB"
                     /locus_tag="VQU32_000886"
     CDS             complement(19550..20746)
                     /gene="pitB"
                     /locus_tag="VQU32_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus major pilin PitB"
                     /protein_id="HET2472361.1"
                     /translation="MKKTFFKKLFTASIAAITALSVFRGVPTFADDNSAITKANGENN
                     AVVKINKTLNIAEGITTPTATFTFKFTEKTGQSSNGAPYQTGVAIPDRNVEYNKNDHP
                     TADKIQKATEDIFSGVAYGHAGEYVYDVAEAKTGWQAITKNGKTIDAMRYDKRTYEMH
                     VIVKNKVNGGVYISSVYFKENNKSNAPKVEPSEQGVYNLFDNTYTKDASKEPNPDDPS
                     QVDPNAKALTITKKVDGASGDKTRDFQFHIKIQLPSTNKTAETPVTNIIVKHGSKSEV
                     LAVVTPADTVEYNFTLKDGETFTVEQLPAGSKYTVTETGVAGYTDSSIYTTNGAEQTS
                     QGQKNVDFTLTDILIGEKKNDNKVTNKIDDVTPTGLLIDNLPFILMIGLGLAGFVVLS
                     KKRREA"
     gene            complement(20791..21342)
                     /gene="sipA"
                     /locus_tag="VQU32_000887"
     CDS             complement(20791..21342)
                     /gene="sipA"
                     /locus_tag="VQU32_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system signal peptidase SipA"
                     /protein_id="HET2472362.1"
                     /translation="MLLKKKHKKPVTQVNRDKSPPSVWGDILYLVSKLLMVGFVLAIL
                     YFFVFGLLRYNDDGMKPALKDGDLVVYYRLDKRYSIGDLLVYSYKGKERVARVIATEG
                     STIDINENGLIINGSPQQEQDIYKETLLYKEGATFPMKVPAGQLFVLGDNRTTAVDSR
                     AFGTIPIQDTQGKVVTVIRRRGF"
     gene            complement(21342..23105)
                     /locus_tag="VQU32_000888"
     CDS             complement(21342..23105)
                     /locus_tag="VQU32_000888"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472363.1"
                     /translation="MIDTPYWFTSGLERGMLNISSLIAPKIDGFYSIKFRYYIGSNDS
                     ITSLKGYISGYNSGIPNEIFSANNENDLQQKFKEITDKILPLGVHHVTISDVLSKYVQ
                     LLPGDASHLRVVKIKDGNEQELNDNQVTIETKKNEQGLVEVTAKFNPSYTLEDDAKYV
                     LKFTVTSSQEAFDAIAGDKTLTSDDAEEADATKLYSNKGAKVAYSYGIGTSRTKIKDY
                     SEKPTFKPSDPLTVPVEIEWKGVDGKSNPSANRPPSVELNLNQKKDGSIKDSYRKVTS
                     PVQTNSFTENTSFAKVAKGYDYELKAPDAPGYTVEVQKTGTKEKPSFKVIYRQLPSLT
                     VKKILEGEQSPNKSFTINVTFSDKDGKPINGKFGNTTVTNGKAQISLKNSQETALSYL
                     PRDTHYKVEEVENSRTGYHVTYEKQEGTLSEDVQTIVTNHRLPTLSVTKKVTGAFANL
                     LQSFKITINVKDAQNKPLNGSYSAIVNNQKTTLQFTNGKATVDLKKDKTIKILDLPLN
                     ARYSIEEEASSSRGYQVSYDKKEGTLDANKSATVTNNKNSVPETGIDFLSSTLVLGVV
                     LPLGGIFFIILLGHLVVNRRK"
     gene            complement(23427..23930)
                     /locus_tag="VQU32_000889"
     CDS             complement(23427..23930)
                     /locus_tag="VQU32_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HET2472364.1"
                     /translation="MNVQYDFKKIQYFTSSLVIFLAILFLCAPINSLRADSITEPQTT
                     LHKTITPISGQKDQYELSLDITSKLGTETQSEPLDVVLVADLSGSMEERDVWSYSSRR
                     YISRIEALKHTLKGVNGRQGLIDTILSNSQNRLSIVGFAGKIDNQYNDRYYNEYYLSY
                     QYGTWPN"
     gene            24289..25512
                     /gene="pepT"
                     /locus_tag="VQU32_000890"
     CDS             24289..25512
                     /gene="pepT"
                     /locus_tag="VQU32_000890"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HET2472365.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPRQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(25548..26177)
                     /locus_tag="VQU32_000891"
     CDS             complement(25548..26177)
                     /locus_tag="VQU32_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472366.1"
                     /translation="MNKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSANSKDKTALLESTKAILVLNHQIQSKVSASEETSPARNAEANSDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            26142..26363
                     /locus_tag="VQU32_000892"
     CDS             26142..26363
                     /locus_tag="VQU32_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472367.1"
                     /translation="MDLFQELICLVQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(26634..26982)
                     /locus_tag="VQU32_000893"
                     /pseudo
     CDS             complement(26634..26982)
                     /locus_tag="VQU32_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(27087..27647)
                     /locus_tag="VQU32_000894"
                     /pseudo
     CDS             complement(27087..27647)
                     /locus_tag="VQU32_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(27873..30992)
                     /gene="phtE"
                     /locus_tag="VQU32_000895"
     CDS             complement(27873..30992)
                     /gene="phtE"
                     /locus_tag="VQU32_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HET2472368.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQISQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         8835 a         6360 c         5686 g        10121 t
ORIGIN      
        1 aaaaggatct agtttttatt tttagttaaa ttatgtctat atttttcatt aactcatcaa
       61 taaaaatttg tttgctaata tctctttctt ctgattttgt ttcaaggatt attgctacat
      121 cattcatatt tttaggtaaa taccaaccat ctggcttatc tgaatatccc ttgaagccta
      181 attgattaaa agtagttatt tgaccagtac cttgattgat gttttcttca acttcattaa
      241 agcctatact tctcttgcat aatccctaac ttgatcttct gtccttaaag tcattttcta
      301 ttcctcgtca ctaaaattat cttcaaaact aataatatca ttaggagtgc aagataaagc
      361 cttacatatt ctttctaaat tcttaaatgt aattggttta tcctttccca tttgtgccat
      421 aacatttgtt gtaagtcctg ccatagctat tacatctgtt ttctttagtc ctttttttgc
      481 taactgtatc cataatggtt tatagttaag taccataata tccctctttt tttcttcatt
      541 aaatttattt aattatagca taaatattga tttcattcaa tcttacattg atttagtgat
      601 ttgttttaga gaatttgttg tgttattccg tccttatttt gccataaatt gttttaagtg
      661 ggacctctta tccttaccat ttttacctcc taattttgag tataaaaaag acgatagagt
      721 ttttaatttc tatcgtctga gatttacata ttaaattttc atcacaaaat aagactcttt
      781 aaagaatctt taaattcatt ggtatgctgc ttacaatatc cattattaaa atggatacta
      841 taagcagcac accaactatt tattttttga gttttaaact ttggtagaag tcttgaactc
      901 cttatattta cataactatt tgcctctata actattatga cacgaaacca acaagcaagt
      961 tcaagcatct ccatatcttt attcaaatga ggactaagat attcacaaac tttatcataa
     1021 acctctttat cttttttcat aaaaggggca tacaacttag caaatttatc ctaaaatttt
     1081 ttgttgcctt tatcaatcat ttcaacgctc ctttaaatat aaaaataatc ctatcaatct
     1141 ttatattgag cctattttct aattgatacc ttgcaagaaa ggaataatag ctgttcctat
     1201 accaagtagg aatgtaataa atgcagaaag tcatatactg tcattctttt ctattaaatc
     1261 attttgaagt gtctcaaagc atccatgatg gcttcttctt tttcaggtgt acactgtgga
     1321 ataatttgtt tatccttttt agacttgttg taatgttctc gtaattctat tccacatttc
     1381 ttttttatct gtgcaatata taatgtcgaa acttttaatt caaatttatt ccaaacatat
     1441 tctttgattt gagcatatgt tgctttgctc tccgcacttg tcaaatccat ctcatccagc
     1501 tcaatttcaa cacttatgtg cttatcgaca tcgagtttgg acaaaagtgc taccgtctcg
     1561 acgtgatgcg tttgaggaaa tagatccacc ggctggactt tcttcaattc atatcccaat
     1621 tcttggtaga gtttgatatc acgcgccatg gttgcgacat tgcaggagat ataggcgatg
     1681 cgatcggctc ctgtttgggc gcttgctttg ataaagcttt ctgtcaagcc cttgcgtgga
     1741 ggatcaacca agataacggt tggttgaata ccttccttga gccatttctt catggcattt
     1801 tcagccgtgt cacagacata gtgggcatta gtaatcttgt tcaaagaagc attcttctgg
     1861 ctattctcaa ccgcttctgg aatcagttca acaccgtaga cttctttgac atgcttggcg
     1921 actgataaac caatggttcc aataccagaa taagcatcaa taaccacgtc atcttttttt
     1981 aactctgcaa agtcaatggc tgtttgatag agtttctccg ccatttcagt attgacttgg
     2041 taaaaggctg ggccagcgat ttggaagtca tttcccaaca tctggtccgt aatatagtct
     2101 tgaccataaa gagtgcacca ctccttacca aaaatcgcat tggtattctg gtcgttgata
     2161 ttttgcatga cagacacaat ctctgggaac tgcttgataa cttgttcaat caattggtca
     2221 acacgaaaaa cttttggacg agttgtcacc aaaacgacca tgatttgtcc tgaatagtga
     2281 ccacgacgca ccacaagatt ccgaatcaat ccagactgtt ccttttcgtc ataaggtttt
     2341 aaatcaaaac gacggagcag gtctcgaaga gctactacga cttggtcaat gacaggatcc
     2401 tggataaaga aatcttcaag gggcatgagg ttgtgcgaat tcttacggaa aaatcctgtt
     2461 tccaagacac cattcactcg acgaacgggc acctgcgcct tattgcgata cttgactgga
     2521 tgttccatac caagcgtttc agcaacttct acatctgcaa ttccagcaat cttgtagaga
     2581 ctgtccttga cttgcttggt tttaaacttg agctgttctg gataagaaag gtgtcctaaa
     2641 tccgcgattc ctgaacgcag gtaagccaaa tctagatctt gattacggtg tagtgactgg
     2701 acaaggtatt tttcaacttt tccaaagcca atctttttat tgaccttgag gacacgcatg
     2761 agaatttttt cactcggtaa agcattctct acaaaaaaga ccaaaccatc taccttggca
     2821 actcctgccc cttcatgggt caaatcaaca atttcaactt ctacaatatc atttttcttt
     2881 aacattcttt tctctttcta ttggccgaca aaaaagacgg caacgttacg attaccatcc
     2941 attataccat gaaatttctt aagaaccaaa actcttgaag aagttgacaa gggcttgcca
     3001 gagggagctt aagaagttac caccgtcctc tagcgcctta tccgcatcaa agttaaggtt
     3061 gatattttta aaactgtcgc cagcttgtga tacgatgctt tgtttaaggt catttagggt
     3121 tttagtgaaa tctgcattgc tgaggatatc actctttgag agattcaagg caaaattgat
     3181 gatgatattg atctggtttc ctgttatgac ctgatcaagt ttgtaatttt ttaaggtatc
     3241 ttcaacaatc ttgcggacat cttcttctgt cagatttccc ttgctttctt tagctttggc
     3301 gagtcctgac ttgatatcag ctagggcaac gtttaattta ttagcatcat agcctgattt
     3361 gtccttgttt tcagcattga tatctgacaa agcctttagc tcttcttgag ccaaatcttt
     3421 attggcttgc ggcaccttgg ctccattagc ttctagcgaa tagtagatcc cagccaaagc
     3481 actctcacct gtaactggaa taggggctgc tacagtgatt ttggcatgtt ccatacccag
     3541 cgttactgct gcgtttcggt acatatcctg agtcacctta gtgatatttt ctggtgtttc
     3601 aatcttgacc tcaagtggcg atttgtcacc tagcttttga atcttggctg atgaatacaa
     3661 ctgtaagcta gagtcattgg ccacattcat gattttagaa taaacatcag gtgtcatggt
     3721 cttgagttct ttggtatctg ttgaggcatt gtagcccagt tttttaagag tttgattttt
     3781 ttggtcttca gatagggagg aacctaggac atattcaggt tggacatagg tttcatcgat
     3841 aaccttttga acatctgttg ctgcatggac gctattcata gctgttactg cccacaagac
     3901 cgcagcactg gtcagaaaga gtttctttct catagggaat ttcctccttt acttttctag
     3961 agtaatatat ctatcttaaa gaaaacttat aacaaaaaca cctggtctag ccagatgttg
     4021 aaaagagagt gaaacatttg atgatgtaaa ggttaagtcg tacctgtcta gaataataat
     4081 agtttcctcc atttacatag agttcagcac cgtgaaaaat ggaaatgggg tgaatataac
     4141 tataagtctt tccagtcgta ttaccaagca agggggcaac agtctcacga gagtactgtt
     4201 tggctagagc caaggtattt tccttgccat tttgggcgat aaaatcgata taggcaggtc
     4261 caaaattata ggcttgaaca gctgtccaga tatctacccc cttcttctgc gccagataga
     4321 gattgcctgt cagagtttga atgccttgcc gaatgctaga ggcattatca ttgatggtgt
     4381 tggttgaacc acttgcagac tcactagact gcataacatc gccttctttt ccttttgttt
     4441 cagtataaat catagcaagc acaagctctt cgtttgctgg ggtgtctttt tcactcaaga
     4501 tttctcgcac catgggttga taggtcatga cttgtttgac atcttgatga acgcggtaag
     4561 ctttatagcc agcaaaaagg aagactgcta gtacaagcac tcttcgaatt cgtttaaaca
     4621 ttatttactt tggatatcct cgatattttt gattaagata gagtaggttc cattttcctt
     4681 ttggataaac tcaacagact cggcgtcttg atagacgtta ttgggaacga tgagctcaat
     4741 tccatttgat aaggagagtt tttggttttc aaatttcttt aattggcgac tggcatcaat
     4801 ttcatcaaat tgaacaggtt ctggtacggc ttctttgact tggtcaataa agctcaaacg
     4861 agccgtcaga ttgttgtcaa aaaggtcatt agccaatttc tcaggtgaca attcattgct
     4921 ttcttctagg ttgttgaaaa tcgctgattt gaccttggat tgaaattgaa aatcatctgt
     4981 gttaaaagat tcagcaattc tctgggctgt tttttccagt tccttgatag attttttagg
     5041 agaaatctta ggagcgacag caagaagatt atctgaaaaa tagttcaaaa aagtcccgtt
     5101 gtacttgatt cgtttttcaa tcaggtgata cttgcgactc tgaagattga ccaccaaggc
     5161 ctcgtcagca cccgttccaa atccaggcag gttattctga gtcagcttga ttggattatc
     5221 aacttctcct ccgaggtggg tcaaggtctc ccgcagggca attcgcaaga aagcgaaatg
     5281 ttctacacct tctttagaaa attgaacaaa aatcaagtca ttggtcttga gattttcaga
     5341 aatgctaaac tcctctttcc agagattagc cagcgttact gatgtctcca acaaatcgtc
     5401 tgtaatatga ttgaagaagg gattttcttc ttcgaaaatc ccagtcttgg cttcatctga
     5461 atacacatgt tcaatttttt tacgtaggta ttcttcgatt tttggagtaa tattgagaaa
     5521 cttatctgct aagaacagct cggtatcatc cggactgaac tggtgaataa tggctttctt
     5581 aatataaatg tccataaaag ttttattcct cgtataatgg gaaggcatct gtcaattctt
     5641 tgactgcact tctcacttct tctaatacag cctcattttc tgaattctta agggttttaa
     5701 tgatgagttc agccactttg cgactttctt cttcaccaaa tccacgtgca gtaatagctg
     5761 ctgctccgat acgaatccca cttgtcttga atggtgacaa ggtttcgtaa gggattgaat
     5821 ttttatttag ggtaatattg acttcatcca gcaagttttg agcaactttt ccgttttcta
     5881 caaccttagt cacatcaaca aggaagagat ggttttcagt tccacctgaa ataatacgga
     5941 aatcagggtc ttgcaagaag acatctgcca tagccttgct gttcttaatt acattggcag
     6001 catattcctt gaaggctgga tccaaaactt ctttgaagga aactgcctta gccgccacaa
     6061 catgctctaa aggaccgccc tgaatacctg ggaaaatagc tgaattgatt tttttagcaa
     6121 gttcttcgtc attggtcaaa atcaaaccac cacgaggtcc acgaagggtt ttgtgggtcg
     6181 ttgttgtagt gatatgagcg tatggcactg ggcttgggtg aagaccagct gcaaccaagc
     6241 cagcgatgtg ggccatatca accataagct tcgcaccgac agcatctgcg atttcacgga
     6301 attttgaaaa gtcaataatt tgagaatagg ctgaagcacc agctacaatc agttttggtt
     6361 ttacttcttg ggcttgtttc aagatagcat caaagtctaa gagttccgtt tcaggatcaa
     6421 cactataaga aacaaagttg taggtttgac cagagaagct aacaggagcc ccatgagtca
     6481 aatgaccacc tgatgccaaa tccattccca taaccgtatc acctggctca atcaaggaca
     6541 tgtaagccgc acagttggcc tgacttcctg agtgaggttg gacattggca aatttagcac
     6601 cgaaaatttc ttttgcgcgt tcaatagcaa gagtctctac aacgtctact acatcagttc
     6661 caccataata acggcgtcct gggtaaccct cggcatattt atttgtcaag atagaccctt
     6721 gagctgccat aacagccttg gaaactacgt tttccgaagc aattaactcg atattatttt
     6781 gttggcgttc ttcttctttg gcaatagcat tccagagatc agcatcatat gctttaaaat
     6841 catctttgtc aaaaatcata ggtcttctcc tttattgtgt gactagtcca ttagtttgat
     6901 tttacaataa gaaaatcaaa ctaaaagatg cgaataaacc gtttctgcat tttatcacaa
     6961 gtatagccaa ctttttcata aaatgcatga gcacccagac gatgattggc agaatttaag
     7021 cggataaacc cataaccaca tcttttggct tcttgttcca acccttgtag taaactttta
     7081 ccgatacctt gaccttgcgc ttgaggtgaa actgctaaag ctaagatatt aaatcctgct
     7141 ttggaataga gtgattcgta aacttcagcg tggacatatc caagtaagac atgattagct
     7201 gcatcctcat agccaagtag gaaatgatgg gaatcctgag acagtctagc tagttggcta
     7261 gccgtttcct ctggactaaa agtataaccc aaagcctctt ggttgatgtc acatatcgct
     7321 ttcacatctg tttcttgcaa atctcttagc atttcaagcc tcctcaaaag aaatctctgg
     7381 caaccgagca agaatatctt ctcgtttaat tgcgccttgg cgtaagattt tcaccttgtc
     7441 tccagacaaa tccacaatag ttgaatcctg tccagttaga aaagcatcgt cttccagacc
     7501 cagaacctct tggtcaaaat ccttcagaat ttgttcaaag gttacaccac ttgcctgacc
     7561 tgagatattg gcagacggcc caatcaaggg acctgtctct cgaattaaat ccagtgtgat
     7621 agggtgactg ggcatccgaa atccaatagt tgcaaggtca gaatttaccc aatagggaac
     7681 tcggtcattg gcttcgagaa taatggtcaa gggacctggc aaaaaggtct ctacaagttt
     7741 ttgtagataa gttggctgat tcttagaaaa gtgcaagata tcctcgaaag aggcgatatt
     7801 gagattgagt gccttatctc taggacgacg tttgagttgg taaacatggt caactgcttt
     7861 ttcatctaag gccttggcaa aaagaccata aacagtctct gtaggtagaa cgacagctcc
     7921 accattttcc aactcttgtc taatcctgtc catcatcaac cacaaccatc ctattttgac
     7981 caaattggtc tttgagtgtt cgaacttttt tttcaggaag atgttttcta aaaagttcgg
     8041 gaacactttg accttgcttg tatccaattt caaggtaaat cttaccacca tccttgagat
     8101 agtcttttgc atcttccgca attctacagt aaatagctag gccatcctcg tctgcaaaga
     8161 gagctagatg aggctccgaa tgcaaaacat tcaagcctac ctctgactca tcttcacgag
     8221 agatataggg tggattggat acaattatat catatttttc agaaatttct gcaaaacaat
     8281 cagatttttt aaaaaatata ttaagatttt gatcgctagc attctctgat gcaagctcta
     8341 aggcctcttg agaaacatct gctgctgtca ctgaccaatc tggtctattt tttgctagag
     8401 cgagagcaat agctccacta cctgttccaa tatccagaac tgagagattc gtctcaagat
     8461 tttcagttag aataagctcc actaactcct ctgtttctgg acgaggaatc aaaacccgtt
     8521 catccacctt taaatgcatt ccataaaaat ctgcctgtcc aatgatgtac tgagctggct
     8581 tgtgagctgc tagttgctgg taaatatctt ctacaaattg tttttcttcc tctgttgtca
     8641 cctcctgctg gagggcaaaa ataaagtctg taaaagatag atttttcaga ctacgataga
     8701 caaaagagag gctttccgct tcctctcctt gtcttatcaa ctcttcttca aaatttgaaa
     8761 ataattgagc taatttcatt atttgtttaa ttcttctagt ttttgtgttt ggtcataaag
     8821 caccaaggca tccacaactt cgtccaattt accagacaaa atcgtatcta gtttttggag
     8881 ggtcaagccg atacggtggt ctgtgacacg gttttgaggg aagttataag ttcggatccg
     8941 ttctgaacgg tcaccagtac cgattgtcga cttacgctca gcgtcttgtt catcctgagc
     9001 aatctgagca aagtggtcag cgacgcgtgc acggataatc ttcatagcct tctcacggtt
     9061 cttctgctgg gtacgttctt cctgcatctc aaccttgata ttggttggca agtgaacgat
     9121 acgaacggca gtcgcaacct tattgacgtt ctgtccacca gcacctgatg cgtggtaaat
     9181 atcaacacga agatcttttg gatcgatatc gtattctact tcttcgactt caggcatgac
     9241 aagaactgtg gcagttgagg tatgaacacg accttggctt tctgtcacgg ggacacgctg
     9301 cacacggtgg gcacctgatt cgtacttaag cttagagtat acagactgac ctgaaaccat
     9361 agcaaccact tctttaaacc caccgacacc attcatagag gcttccatga cttcaaagcg
     9421 ccagccttgg gcttccgcat acttttggta catagttagc aaatctccag cgaaaagtgc
     9481 cgcttcgtct ccaccagctg ctccacggat ttcaaggatg atattcttgt catcgtttgg
     9541 atcctttgga aggagcaaaa ttttcagttt ttcttcatat tcttcttttt cagccttggc
     9601 atctttgagt tcttgcttgg ccatttcttc caagtccgca tctccgcctg attccttaat
     9661 catctcttcg gcatcgacga tattttgaag gacttgttta tactcacggt aggctattac
     9721 ggtatcacga ttggaagctt cttcttttga aagctccata aaacgcttgg tgtctgaaac
     9781 gacatcaggg tcactcagca attctcctag ttcttcataa cggtcttcta cagcttgtag
     9841 ttgatcatag atgttcattt ttcttcattc tccttattga tttcaggggc aaaataatgt
     9901 ttacggcaaa ccgagatata ggtttcatta ccaccaattt ggatctgttc tccatcgtaa
     9961 acgggcagtc catcctgtgt tcgcaacacc atggtcgcct ttttcttgca atactgacag
    10021 atggtcttga tttcgtcaat cttgtctgct aaaagcaaga gatatttgga accttcgaac
    10081 aattcattgc gaaagtcatt tttcaagcca aaagccatga cgggtatgtc taactcgtcc
    10141 acaacacgag ctaggtcata aacatggtgg cgtttgagaa actgggcttc atcgaccaac
    10201 acacagtaag gtttttctgg taggcctcgg atatagccaa agatatccgt tgtttcctca
    10261 atcgcaaggg cagggcgttt catgccaatt cgactcgaca catagccaac accgtcacgc
    10321 gtatccagag ctgaagtcat aatcacaaca ccttttcctt gctcctcata gttataggcc
    10381 accttgagaa tctcgattgt ctttccagag ttcatggtcc cataacgata atacaactgt
    10441 gccatgtttc ttgcttcacg tccatttcta aatttttgct acattctagt atatcataat
    10501 tttcttaagc tttaaacggc aaaatgtggt aaaatggaag aaatcaaaac tagtggagga
    10561 agctattatg ccatttgtac gcatcgattt atttgaagga cgcacgctcg agcaaaagaa
    10621 agctcttgct aaggaagtaa cggaagcagt tgtccgcaac actggagccc ctcaatctgc
    10681 tgtccatgtc atcatcaacg acatgccaga aggaacttac ttcccacaag gggaaatgcg
    10741 tactaaataa gctagcttaa gcagaattgc ttaggctttt tcaatctcca agtagcattc
    10801 attgaagaaa tatcctaaat ttgttacaat ttgaaaagaa acttggagaa tttccaagaa
    10861 aagagctatt aattaaagga aacattatga ttacacgtga atttgatacc atcgctgcta
    10921 tctctactcc actaggtgaa ggggctattg gtattgtccg cctgagcgga acagacagtt
    10981 ttgctattgc gcaaaagatt tttaaaggaa aagacttgaa caaggttgcc agccacactc
    11041 tcaactacgg tcacattatt gatcctctga ctggtaaagt catggacgag gttatggttg
    11101 gggctatgaa gtctccaaag accttcactc gtgaggatat tatcgagatt aacacccacg
    11161 gtggtattgc ggtgaccaat gagattctcc agctagctat ccgtgaagga gctcggttgg
    11221 cagaacctgg tgaatttacc aaacgtgcct ttttaaacgg tcgcgtagat ttgacacagg
    11281 ccgaggcggt gatggatatc atccgcgcca agacagacaa ggccatgaac atcgcggtca
    11341 aacaacttga tggttccctt tctgatctta tcaataatat ccgtcaagaa atccttaaca
    11401 cacttgccca agttgaggtc aatatcgact atcctgagta tgacgatgtt gaggaagcca
    11461 ctactgctgt tgtccgagag aagacaatgg agtttgagca attactaacc aagctcctta
    11521 ggacagcacg tcgtggtaaa atccttcgtg aaggaatttc aacggctatc attggacgtc
    11581 ccaacgttgg gaaatcaagc cttctcaaca acctcttgcg tgaggacaag gctatcgtaa
    11641 ccgatatcgc tgggacaaca cgagatgtca tcgaagagta cgtcaacatc aatggtgtac
    11701 ctctcaaatt gattgataca gctggtattc gtgaaacgga tgatatagtg gaacaaatcg
    11761 gtgttgagcg ttcgaaaaaa gccctcaagg aagccaactt ggttctacta gtgctaaatg
    11821 ccagtgaacc actgactgcg caagacagac aacttcttga aattagccaa gataccaatc
    11881 gcattattct acttaataaa accgacctgc cagaaacgat tgaaacttcg aaactacctg
    11941 aagacgttat ccgtatttca gtccttaaaa accaaaacat cgacaagatt gaagagcgaa
    12001 tcaacaacct cttctttgaa aatgctggct tgattgagca agatgctact tacttgtcaa
    12061 acgcccgtca catttccctg attgaaaaag cagttgaaag cctacaagcc gttaatcaag
    12121 gtcttgagct ggggatgcca gttgatttgc ttcaagttga cttgactcgt acttgggaaa
    12181 tcctcggaga aatcactggg gatgctgctc cagatgaact catcacccaa ctctttagcc
    12241 aattctgttt aggaaaataa gaaaaatcca tgatccttca ttcggtcatg gattttattg
    12301 tctttattag taatctggtc ttaagacccc tgttacagtt gccttagttg cttcgtagtc
    12361 gccatctacg acaaccttga taatgcgttt ggcatcttct tctggtgctg gaacaagagg
    12421 tagacgagtg ggtccagctt caaatcccat atagttaaga attgccttaa ctggagcagg
    12481 acttggataa gagaagagag cattaacctt aggaatgaat ttacgctgaa ttgctgcggc
    12541 tttcttcata tcgctttctg caatggcagt aaacatctcg tgcatttcat ccccatttgt
    12601 atgagaggca acagaaataa ccccatccgc cccaaggttc atggcatgga aagcatctcc
    12661 atcctcacct gtataaatca agaactcttc aggcttgtgc tcaatcaagt aagccatatt
    12721 agccaagcta gtacattctt tgacaccgat aatatttgga tggtcagcca agcgaagcat
    12781 ggtttctgga gtcaattcga caactacacg ccctggaatg ttatagataa taattggtag
    12841 gtcagaagca tctgcaatag tcttaaagtg ctgatacatc ccttcttgag aaggtttgtt
    12901 gtagtaagga acaatagcaa gcccagctgc gaaaccacca aattccgcta cttctttgac
    12961 aaactcaata gagtcacgcg tatcattagt acctacaccc gcaatcaaag gaacgcgtcc
    13021 attgacaacc ttttgtacag ccgcaaacaa ctccaactcc tcatcgtggg tcaaagttgg
    13081 actctcagca gtcgttcctg cgagaagaat tccatccgta tgatgggcca ataaatgctc
    13141 aatcaaggct ggaatagcat caaagttaat agaaccatcc tcatgaaagg gggtaataaa
    13201 ggctgtaatg attttacatt cttttaaatc ttgataagac atgaaaacac ctctctattt
    13261 caaagaagga gttcatctga actcctaaac gatatgacta ttttaattca aatttcaatt
    13321 cggctgttgg acgaaccaat ccacgttcat gaagagtttc agcaatctga actgagttcc
    13381 aagcagcacc tttgagaagg ttatctgaaa caacccacat gtgaattcct ttttctgcat
    13441 ccaagtcttt acggatacga ccaacaaagg tatcacgcga accaactgca ttgatagctt
    13501 gaggatagat ttgatgagct acatcatctt caagaacagc acctgggaag gctgcgatag
    13561 ctgcttttac ttcttcgatt ggagccactt cttttgtttc gatataaaca gactcagagt
    13621 gagctgacaa gactggaata cgcacacatg ttgcagatac tgcaatgcta tcatcttcca
    13681 taattttctt agtttccttg gtcatcttca tctcttcgta cgtgtaatca ttatcagtga
    13741 aaacatcaat ttgtggaaga gcgttaaagg cgataggata atgtttcttg tcaccacctg
    13801 aaggcaaaat ttccgcatgc aaatcacatg gtttcacacc atcattcaag acttcacgaa
    13861 gttcacgttg tgtctcaaga attgctccca taccagcacc tgaaacggct tgataagttg
    13921 aaacaatgat acggtccaag ccccattttt ggcgaaccgg ctcaagagcc accatcattt
    13981 ggattgttga acaattaggg caggcaatga ttccgttgtg ggcatcaagt gcatgagcat
    14041 tgacctctgg aacaaccaaa ggaacatctg gattttgacg gaaataagat gtattatcca
    14101 ctactaccac gccagctttt actgcgtatg gtgcatactt agctgatgta gaactacctg
    14161 ctgaaaagag agcaatatca actccttcaa aagctgtttc agtcgtttct tcaattgtaa
    14221 tatcttgatc tttaaatttc aatgacttac ctgctgaacg tgcagaagca aggtaacgga
    14281 ttttatcgat tggaagtgtt gattcttcca acatttttat catctgagca ccgacagcac
    14341 ctgtcgcgcc gactacagca actgtatatc ccataaataa cctctttcgg aattttctaa
    14401 aaatttctat aatagaagta ttatactact ttttccatga attgcaaagc tttttcatca
    14461 ttttttggaa aataaaaatc ccagtcgcta gactagggac taagaggcat cttcatgtga
    14521 tgagcaggtt cacacaactc atcaaggtcc gctcctgcgt tatgacctcc ttatgctcaa
    14581 tagtagtccg aagactacct atcgactcat cgcatattct atattactag agatacttga
    14641 ttttgtcaat aagaaatttt ttcgatcgag tataacaaaa aactctcgta ttcaagagtt
    14701 tccctataca tatcatgcta attgccggga tcgaaccggc gacctcatcc ttaccatgga
    14761 tgcgctctgc caactgagct aaatcagctt acttaagaag tatactatat tcagaaaaac
    14821 ttgtcaagtt tccttagaaa ttttccatag gaaaaagcca ggtaagaact acctagctta
    14881 tcttcttcta tttacttaaa tcaattcgac gaccaatttt accaataata atcgccccaa
    14941 taatcaatga agcaaattca ccgattcctg ttgtaaacca agtaaggaag aatggtaatt
    15001 ctgctacaat atttaactct gcagcgatag taaacatgga aattgaaaag aggattgaaa
    15061 agaagaaatg atcttttcga atcaatccgt taaagaggta atccttgcta tattttgcaa
    15121 aaagccaaac acctaaactg aggaatacta aggttgaacc accaccaaca aagacatcta
    15181 gcagtccgaa gctaaagaaa ttagcaatca tacaaccgat ggtaactccg atgatgtact
    15241 taggattgta aaaagccata aagttcatca tttctgaaat acgaaactga taagcaccat
    15301 agctgatggc atttaggggc ggtgtgacag tcaaaacgac gtagatagca gcgacgattg
    15361 caatatctgc aatatcacga atagttaatt ttttcatctt ttctcctttg gcggttgccc
    15421 gcgtgtaata tgcttggtga aagaagctaa gcaccaaggg ttgctttatt caaccctact
    15481 agtatagcac aatagcctgc tttatgctat acttaacaca tgaaaattcc tattcaaaaa
    15541 ttttttaaca atgaaatctt agcctatctc ttttttggcc ttgcgacaac tctagtttcg
    15601 attctctcac gactagtcat ttatcaacta agtcatcagg aactcctggc tactgcccta
    15661 gcaaatatta tcggtatcct atttgccttt atcacaaatg atacgattgt atttaagcaa
    15721 aaaaagaaaa atagacttat ccgtttagtg aaattttctc ttgctcgcct ttctactttt
    15781 ctgttagact tgtttttaac ttttctcttt gtaacccaat ttcctcacat tatagggcaa
    15841 ttcgtaactg aaaacattga taagataaat gctattgaaa caataattgc acaaatattc
    15901 attattatca tcaattatat attgagcaag gcctatattt ttagaaaata atgctccatt
    15961 tatgagcctt ttaattgcct tctataatga tttaggatac aatgaaacac gaaacattga
    16021 aaggaaaaca gtatgttaaa aaatctaaaa tcgttcttgc ttcgaggaaa tgttattgac
    16081 cttgctgtcg gtgttgtaat tgcctctgct tttggtgcta tcgttacttc acttgtaaac
    16141 gacattatca ctcctcttat tttaaatccc gctttgaaag ctgctaaagt tgaacgtatc
    16201 gctcaacttt cttggcatgg agtcggctat ggtaacttct taagtgctat tatcaatttt
    16261 atctttgtgg gtaccgccct cttctttatt atcaagggca ttgaaaaagc acagaaactg
    16321 actggcataa agaaagaaaa aactgccgaa aaaaaaccaa ccgaattgga agtccttcaa
    16381 gaaataaaag ctctccttga gaaaaaataa tcgattaaaa ggatctagcg caaagctaaa
    16441 tccttttttc tttactcttt gggtaacttg cctgcttttt caagcatttt tttgatcaga
    16501 taaggcatct taacattctc ccgccctttt ttctcaatca gttttttcac aaattctggc
    16561 atttgtaaat cttcagattc agctaaaatg tttttcgtct cgtctgaagg aactaactca
    16621 tcaaaggtct cttcttctgg gagaaattcc ttaaactctt gattttcatt gactctaact
    16681 gtattgacca aagcatcaat caaacgagaa tctgtatttg gcattggtgg acgatggtag
    16741 tttaccccaa attcctggca taagtcataa cattctacat cattgtcaaa caagacctca
    16801 atatgctcac tgatgaaact aataggtaca aaaatataat ggtctggatg ttctgtctgt
    16861 tctctgagat actccaaaac atctggttta atccatggaa taccaatatc actctcactc
    16921 tgccaagtgt tggtatattg ttctgaactc aagcccagtt tttcagcgac tagcttgcta
    16981 ttttcgaaaa tttggtctat ataaggatca ccaaagtcca aggcaaaaat gggcacacta
    17041 tgggctgaaa agatgacttt aaagctgtct tgctttactt cttcttttaa aatcttagca
    17101 atttcatctg cccaatagtt taagagcact tcttcctgat accagtcctt aatgaccaaa
    17161 aattgaattt gtttgctttc taaaaatttt tcatagccca tgacagagta gaaagaataa
    17221 tggggctcca aaatcaagca gatacattct tcaacaccat cagtttccat ctgcttgatc
    17281 acgtctggaa taaagggacg agaaaatttg ttggcaaagt agataccgta ctcctcccct
    17341 aaacgttctt tgactaaatt gacctcctca cgtgtaatcc gctgcaaagg cgtcccaccg
    17401 atacgaatat agttgtcata gagagtctgg atttcgtggt cttggggtct aactccacga
    17461 cgaatgtttg taaagaattc tgctactcct tcaaaactaa tctcctctgg agatccaaag
    17521 gtcatcatta atatggcttt tttcatgtta tcatccttat gatattattt tctctatcca
    17581 ttctatcatg aaagaaaaaa atagtaaacg tttcctacgt ctactatttc ttttgctctt
    17641 ttgttttttt atctcgtctt ataattagga tgataaggac aagaagtaag aggacgataa
    17701 taaggagtag aaaggctaga atgtaaatcc aatgactggg aattccgtga tcgaaatacc
    17761 gtgttcctac tgcttcccca ctttcatcct gtgctacttt caccggtgca tccgttattt
    17821 tagctaaaag gatatgtcgt ccattagtta tgttaaggaa acatctcgct tgtacttggc
    17881 tttacttagc agatattgat agtaagcctg gtgttcctca tccttggaac tcaaggttcg
    17941 ataaatctcc gtgtcatagg catccacttc taaaatccca aaaatttcaa gaccacgttc
    18001 tcgaccattg tagtagatag aaccgtagcg atgctggtcg aagaattcct tatcagcaaa
    18061 tttcttaatc tcaccgaaca tagcccctga agccatagag tgtccgtaga tgatcgtatt
    18121 aaaatcagta aagttgggat taaagcgata gtcgagaaaa attgatccca ttatcgtata
    18181 atcgccaaag gcattgcggt caaggtattt gacattatcc tgactttgta gaagcggata
    18241 gtctaccttg gtcccatcaa ggctaatcca accgcaaaca tctttgttca agtgctgtag
    18301 ggtttcaaaa cctgtgctcg aagcatcctt ttcaatagga ttatagtgat catagtcact
    18361 tgcttctact actctctgcc aactaaaaag agctacaaaa acacagaaga ataccagaaa
    18421 tacgttttta aatcgcgctc ttttttgaac cgtcatcagc actcctttct atgcgagatt
    18481 tcttaaaagg aaaggaggcg ggacaaaacc gcctcttttc tattcaccct tagtcagtaa
    18541 aagggtctgc ttcttgcatt ttttttactc tctcttctac gacgtaggat caagtagagg
    18601 acaaggagta agaaaatcaa gagcaagaac aatacaaaca agataaggta ccaaatccat
    18661 agtgggattg atgagaggaa acgtccaaga gacgagtcat caaagacact gtatggaaat
    18721 ggttttgatt tttttgtatg gaaagtattt ttagggacgg tgtctgtaat ctttgcgact
    18781 acgatatgac gaccattggt cacatcgaga aaacaggtac tgagtaggat gatacgatca
    18841 ctcggtgaga gtgagatatc ccgcttgtgc atggcgactg agagcaggtg gtctagatag
    18901 gcctgctggc ggtcctcacc ctgtattcct ggatcataga tgttaaagtc ataggcatca
    18961 acctccaaca tctcaaagat ctcgagccct ttttccacac cattgtagta tatactaccg
    19021 taacgatgct ggtcaaaaaa ttcctgatca gcaaacttag ccacatcacc aaacatgacc
    19081 ccattttcta cgtggtgccc gtagataatg gtattaaagt cttcgaactt aggattattt
    19141 cgtgcatcga gaaagatagc gcctgtcgct gcaaactcac ctttggagtc cttgttgaga
    19201 tacttttcat tgtccttggc ttggactaag ggatagtcga tattggtgcc atagacattg
    19261 atccaaccga ggacttcggg attgagttct tggagtttgc tgaagcctga aaaactggcc
    19321 agctcatcct gttgcgtcgt gacaggtcga taagcttcgt actcacttga cgaagctaag
    19381 gagtagactt ggttggaatc ccaaagggcg tagaaaccaa agagaaagat caaagctgca
    19441 aaaatgagca gcaagtgatt gatcacccca tccacagcaa gaacgagacg cctagacaga
    19501 ctcttttttg gtttctcacg cttggttttc atcatctgcc cccagccgtt taggcttctc
    19561 tacgtttttt agacaagaca acaaatccag ccaaaccaag accaatcatc aaaatgaatg
    19621 gaaggttatc aatcaagaga ccagtaggag taacgtcgtc gattttgtta gtaactttgt
    19681 tgtcgttttt cttttcacct atgaggatat ctgttaatgt aaaatctaca tttttttgtc
    19741 cttgagatgt ttgttctgca ccatttgtag tataaattga tgaatctgta taacctgcta
    19801 ctccagtttc agttactgta tatttagaac ctgctggtag ttgttcaact gtaaatgttt
    19861 caccatcttt aagagtaaaa ttgtactcaa ctgtatctgc tggggtcact actgccaaca
    19921 cctctgactt agatccatgt tttactataa tattcgtaac aggggtttct gctgttttat
    19981 ttgtacttgg aagttgaatc ttgatatgga attggaaatc tcttgtttta tcccctgaag
    20041 ctccatcaac ttttttagta attgttaatg ctttcgcatt ggggtctact tgactcggat
    20101 catcaggatt aggctcctta cttgcgtctt tggtatatgt gttatcaaat aaattataaa
    20161 cgccttgttc acttggttct actttagggg cgttagattt attattttcc ttaaagtata
    20221 ctgatgaaat atagacacca ccatttactt tattcttaac aataacgtgc atttcatatg
    20281 tacgtttgtc gtatctcatg gcatcaattg ttttaccatt tttggtaatc gcctgccatc
    20341 cagtttttgc ttccgctaca tcataaacgt attcaccagc atggccataa gcaactcccg
    20401 aaaaaatgtc ttctgttgct ttttgaatct tatcagcagt tgggtgatca tttttattgt
    20461 attctacatt tctatctgga attgcaactc cggtttgata tggcgcaccg ttagaagatt
    20521 gtcctgtttt ttctgtaaac ttaaatgtaa atgtcgctgt tggtgttgtt attccctctg
    20581 caatattcaa cgttttatta atcttcacaa cagcattatt ttcaccattt gctttggtta
    20641 ttgctgaatt atcatccgca aaagtcggga cacctctgaa tacggacaaa gcggttatag
    20701 ctgcaatgct tgcagtgaat agctttttaa agaatgtttt tttcatgatt atttcttttg
    20761 tttttttatt ttcttttttc attatgatca tcaaaagcct cgtcttctaa tgactgttac
    20821 aactttgcct tgagtatcct gtatagggat ggttccaaaa gcacgactgt ctacagccgt
    20881 tgttcgattg tccccgagga caaaaagttg tcctgctggg actttcatcg ggaaggttgc
    20941 cccttcctta tagagcagcg tttctttgta gatatcttgc tcttgttgag gagaaccgtt
    21001 gatgatgaga ccattttcgt ttatatcgat tgtacttcct tcggttgcta tgacacgcgc
    21061 cactctttcc ttacctttat aactatagac tagcaaatca ccaatcgaat agcgtttatc
    21121 caacctataa tagacgacca agtcgccatc ttttaaggcg ggcttcatgc catcgtcatt
    21181 gtatcttaat aatccaaaga cgaaaaagta aaggatggct agtacaaatc caaccatcag
    21241 aagtttactg actaagtaaa ggatatctcc ccagacactc ggcggagact tatcccgatt
    21301 gacttgtgtt actggtttct tatgtttctt tttaagcagc atcacttcct cctattcacc
    21361 acaaggtgac caagtaagat gataaagaag atccctccta gaggaagaac gactccaagc
    21421 acgagagtgc tactcaagaa gtcaattccc gtttcaggta cgctgttttt attattcgtg
    21481 actgtcgcag acttatttgc atcaagagtt ccttcttttt tatcatagga cacctgatac
    21541 ccacgagacg aacttgcttc ttcttcgata ctataacgag catttagagg aaggtcgaga
    21601 atcttgatgg ttttatcttt ctttagatca actgtcgcct taccattggt gaattgtagc
    21661 gttgtttttt gattatttac tattgcacta tacgatccat tcaatggttt attttgcgca
    21721 tcctttacgt taatggtaat cttaaaggat tgcagaagat tagcaaaagc acctgtaact
    21781 ttttttgtga ctgaaagtgt cggaagtctg tggttggtga cgattgtttg aacatcctct
    21841 gacaaagtcc cctcttgttt ttcataggtg acatgatatc ccgttctaga gttctctact
    21901 tcttccacct tatagtgggt atcacgaggc agataactga gggcagtttc ctgactattt
    21961 ttgagagaaa tctgtgcttt cccgttagtc actgttgtat tcccaaactt gccgttaatc
    22021 ggcttgccat ccttatctga aaaggtaaca ttaattgtga aagatttatt aggtgattgt
    22081 tcaccttcta ggattttctt tacggtgaga cttggaagct gtcggtaaat aactttgaag
    22141 gatggtttct ctttcgtacc tgtcttttga acttcgactg tgtatcccgg agcgtctggt
    22201 gctttcagtt cgtagtcata tcccttagct acctttgcaa aactagtatt ttcagtaaaa
    22261 ctattcgttt gaactggact agtgaccttt cgataggaat cctttatact tccatctttc
    22321 ttttggttta agtttaattc gacactaggt ggacgatttg ctgatggatt tgattttcca
    22381 tccacacctt tccactcaat ctctacagga accgtcaatg gatctgacgg cttgaaagtg
    22441 ggcttctcag aatagtcttt tattttggta cgtgaggtcc caataccata ggaataggca
    22501 acttttgccc ccttgttgga gtagagttta gtagcatcgg cttcttcggc atcatcacta
    22561 gtaagtgtct tatcacccgc aatcgcatca aatgcctctt ggctagaggt gacagtaaac
    22621 ttgagaacgt acttggcgtc atcctccaaa gtgtaactcg gattaaactt ggctgttact
    22681 tccactaatc cctgttcgtt cttcttagtt tcaatcgtaa cttgattgtc attcagttct
    22741 tgctcgttac catccttgat tttgacgaca cgaaggtgtg aagcatcacc aggtaacagc
    22801 tgcacgtact tggacaagac atctgatata gttacatggt gtacgcctag aggtagaatt
    22861 ttatctgtga tttctttgaa tttttgttgc aagtcatttt cattattggc agaaaatatt
    22921 tcgttgggga ttccagaatt ataaccactg atatatcctt taagagatgt gatactatcg
    22981 tttgaaccta tataatatct gaatttgatt gaataaaagc catctatttt tggagctatt
    23041 aaactagata tattcagcat tcctctttct aaaccagagg taaaccaata tggagtatca
    23101 atcatacgtc ccacatttgg attagaataa tagttatata ttgttctccc actattagac
    23161 tcgtaataca tattagcttc gccatctgac agcagaataa ctactttttt tgccccagcc
    23221 cttgcacttt gcaacaatct ttgagcttca gttaacccag catttatatt tgtcccagtg
    23281 ccaatgcccg cgtccatacc ataagaacga ctagagtcag caattgttaa cgaactaaca
    23341 atattttttg agctattaga atccgtgctc caagatacta aagttttagc atcatcatat
    23401 gaagagatat ttgaatacca accagctcaa tttggccaag ttccatattg ataactcaga
    23461 taatattcat tataataacg gtcattatac tgattatcaa tctttccggc aaaaccaact
    23521 atagacagac ggttttggga attagaaaga attgtatcaa tgagcccctg acgaccattc
    23581 acacctttca gtgtatgttt tagtgcttca atcctactaa tgtatcgtct actagagtaa
    23641 gaccacacat ctcgctcttc catactccct gaaagatcgg caaccaagac tacatccaag
    23701 ggttctgact gggtctccgt tcccagttta gatgtgatat ccagtgacaa ctcatactgg
    23761 tctttttgcc ctgatatcgg agtaatcgtt ttgtgcagag ttgtctgagg ttcagttatt
    23821 gaatctgcac gtaaagaatt aattggtgca cacaaaaaaa gaatagcgag aaagataact
    23881 aaactactgg taaaatattg aatcttctta aaatcatatt gaacgttcat gctatctccc
    23941 ttgctagaat atgttttaat ctgatactat ataaatctga gacttccata agataattgt
    24001 ataataatat taacttcgtg tcaattaatg tgtttgaaaa catgttatta taaattacaa
    24061 aaaagtatga ttcttctttg tttttcaact ttttctgtgg taaaatggaa aaaatagtaa
    24121 aaaatttcat aaaataaaga tgcatatatt tattctaatc gctcgaaaat ttcttttgaa
    24181 agtcttctga atttgaatta ttatattttt tcaaaattca taaaatttct caaatcatat
    24241 ttttccgaag ttttgtattc tattttatga taaaaaagga gtttttttat gacttatcca
    24301 aatctcttgg accgcttctt aacctacgtt aaggtcaaca cgcgctctga tgaacactct
    24361 actactactc caagtacaca gagtcaggtt gacttcgcaa caaatgtcct aattcctgaa
    24421 atgaaacgtg ttggactgca aaatgtttac tatctaccga atggttttgc tattggaacc
    24481 ttgccagcca acgatccgtc tttaacacgt aagattggtt ttatatcgca catggatact
    24541 gctgatttta atgctgaagg agtcaatcca caggtaattg aaaactacga tggtggtgtg
    24601 attgaactag ggaattctgg tttcaaactc gatccagccg acttcaagag tcttgaaaaa
    24661 tatccaagac aaacactcat cacaacagat ggaacaacct tgctaggtgc tgatgacaag
    24721 tcaggaattg ctgaaattat gacagccatt gaatatctaa ctgctcatcc tgaaattaag
    24781 cactgtgaga ttcgtgttgg ttttggtcca gatgaagaaa tcggtgttgg tgccaataaa
    24841 tttgatgcag aagattttga tgtggatttt gcctacactg ttgatggtgg tccactaggt
    24901 gaacttcagt acgagacttt ctcagccgct ggtgctgaat tgcatttcca aggccgtaat
    24961 gtccaccctg gtactgccaa agggcagatg gtcaatgccc ttcagctagc aattgatttt
    25021 cataatcaac ttccagaaaa tgaccgacct gagttaactg aaggttacca aggtttttac
    25081 catctaatgg atgtgacagg tagtgttgag gaggtgcgtg caagctacat cattcgtgat
    25141 tttgaaaaag atgcctttga agcgcgtaaa gcatccatgc aatctatcgc tgataagatg
    25201 aatgaagaac ttgggagcga ccgtgtcact ctcaacttga cagaccagta ctacaatatg
    25261 aaagaagtca ttgaaaaaga tatgactcca attaccattg ctaaagccgt tatggaagat
    25321 ctaggtatca cgcctattat cgaaccaatc cggggtggaa cagacggctc taagatttcc
    25381 tttatgggaa tcccaactcc gaatatcttt gcaggtggcg aaaatatgca cggacgtttt
    25441 gaatacgtta gccttcagac tatggaacgt gcagttgata ccatcattgg catcgtagct
    25501 tataaaggct aaaaagacga ggtggctcag ctacttcgcc tttcttttta ttctactggt
    25561 ttttcttgat ttccagtagt tgtagaagat tctgttgttt cattttctga agttgattca
    25621 gcaggtttag aatctcttgt attgcttggt ttgttttcgt cgctagcagt ttcaatgtta
    25681 gattctgcag ttgcgtttgg ttggttctca gcactggtgt tatcactatt tgcttcagca
    25741 tttcttgctg gacttgtttc ttcacttgcg ctaacttttg actggatttg atgattcaaa
    25801 actagaatag cttttgtcga ttcaagtaaa gctgttttgt ctttactatt agcagaaagt
    25861 tgatctaata atgcatccac cttatcaaag tccgcatcag atccattatt actttctaaa
    25921 taagagtgaa gcgacatgag aatatcgtag agtttttgat agagtacaag tgtctgagaa
    25981 tcttgctcag cattttcctt ttcttgttga agggcgctag cgatacgagt caaaacatct
    26041 tttacctgac tgtttacttc atccaagtct gcatcagcct tgtttgtggc agcttttaga
    26101 ttttctactt cttctgccaa ggattgtctg attccttctt catggatttg ttccaagagt
    26161 tgatttgcct tgttcaaaag actttctact tcttccttgc tatctgtcgc agattattgg
    26221 ttgctatcta ccatgtactc ctaaaacagg agagttataa tccaagatta caaggcctta
    26281 cagaaataag aaatccagat aagacaatgt tcgtccaaga cgctattcgc ttcgcacagc
    26341 agcacggatt caatatgctt taattttaaa gtttaggtgt caagacctct ttttagtgtg
    26401 cccaaaattt agagaagtaa tcaatcaact aacttttatt tttttcaaac tttcagtaaa
    26461 ctgacctaaa gctaactcaa tctgtctttg tagatgcttc tgctatcagc tagaagttga
    26521 tctacttttg ccaagactgc cttctcatca aaagttccag gttgatagtt ggattgcagg
    26581 gatggaatct tgtttttcaa agccgcttca tatcccttag tttgaacctt gatgtagtga
    26641 ttgtggtcgc catgaggaat cacaaaacct tctgaatctt cacttataat tcgattggca
    26701 tcaaaaacca taaccatctt cttcctcatg gtggacatgt agtgacggat tacttaatac
    26761 agaactagaa gaacttccta cctcttccgt gttagagtgt gatgggggat tgttaagaga
    26821 tgacttagga atatagtgat agtgttcccc atgtcttact atataagcat cacctatatc
    26881 tctgacaata tcattagggt taaagacata tgtggctgct aattcacctg ccgacaagtc
    26941 actctcagga atgaaatgat agtgaccacc atgtggtact attagtagat tgaaatagaa
    27001 tatgagcaaa ttgataaggg gattttaaag taatttctaa caatgattta gaaactatga
    27061 tgtgctattc taaattcaac tcactatata taaccatcat cggtagtata acgtccctgt
    27121 aattttgcta cagatacttc tgcactagct cctttatcgt ctttaccatg ttcttgtttt
    27181 tggcgattga tttcatcttt tgttcgtaca ttttctgcat gagcttgatc tttaaggtaa
    27241 acataatact ttccatctac cttaataata tatcctccct taacctaact gacaatatct
    27301 tgatctttcg gctgatagtt gggggctttc attaatggct cttcactaaa gagcgcatca
    27361 aaaggaactt taccattata gtagtgataa tgatcgccat gagaagttac ataaccttga
    27421 tctgtaatct taataacaat ttgttttgct tgaattcctt ctttttgact aacctagtct
    27481 ggagtcaaat tttcagtctt cttagtgtct ttattactgt ttacatatga aacacgattt
    27541 ttatctgtat tggcctgtta gctatgttgg ttcagagcat aaacacacag acttaaggaa
    27601 aggataacaa cagatccagc tgctatatat ttctttttaa atttcataat tacctcattt
    27661 ctataattat ttatatgatg tcttcattat taaatgatta tagaaattaa ttaaccaatt
    27721 aattaactag taaatattcc acctcttttt aagttgtatg tcaagaaatt ttatatatta
    27781 ataataaaat gaaattctcc caaagtcaga gttttatttc taacttttga gagaacttca
    27841 tttttgattc agactttttc tactgctatt ccttacgcta tgagatcaga taaattcttt
    27901 tttatcactt ctccacttgg caatcttaat tcaatcgttc catccatatt gaatataaca
    27961 ctatctaagc ctaatccgta actagctgta aatttttcta atttttcttg tacaggatct
    28021 actgctggag cttcctctaa tgctggatct aacatagggt cactccccac attcccttct
    28081 ggattcaaca ttccattatc cgttgagttt tctggtttta caggtttttc gtttggtgcc
    28141 tctggtaaag aatctgctgg tttattttct gttggttggt tctcaactgt tccagtagat
    28201 acttttccat tttcagatgg tttattttca ccatttcctt gaggtgcttc tcctgtaaaa
    28261 tctgccatat tctttttaat gacttctccc gatggtaaat ataattcaat tgttccgtcc
    28321 atattaaaca agacattttc tagcttcatc ccataacttt cagcaaattt tgctactttt
    28381 tcttgtacag gatccactgt aggaacttct tctaacgttg aattactagt actattccca
    28441 gtttcagaaa gtttttcttt ttctaccttc tcactagtct ttggttcttc taccttttca
    28501 tcaagttttg agttttcttg tgctttattc cttttaaatt gtggtagaat acttggttta
    28561 tcagtttgat tttctttttc caagataggt acttccacaa tataagtcga ttgattgtcc
    28621 aaataagcat ttgccatgaa ggttacagga attttatttc cggccgttct ggttgttcct
    28681 tggtttaatt tcggaatcgg taatttgatt tcaccaactt tatagttatt ttctaaataa
    28741 gcatttccat gaaattcatc aaacactctg actaaagcat cagttccttt aggcactgca
    28801 aattgagggt tcactcttaa ataagtatcc cctgcatgga aaggatagaa aatcgtttga
    28861 ctggccattt tgtaagctaa agaggttgga actgtaaatg taccatcata acttacttct
    28921 ggataatctt ttgaagcgat agtatactta aatgtttgtc ctggtaaata aggttgatct
    28981 aattcaaagt ttgcaatatt ccctactcct tctccaaata ctttaccaga tactttctcc
    29041 aatacttttc catctggtgt tattaatttt actagcatat tgatacctaa ttttttctcc
    29101 aattcaggcg gaaaactaaa agaaacgcgt ttttgaccat tggctagagt aaagttttga
    29161 ttattaaacg tactattttt taacaaatta acaacattcg ttaattcttc tccagtataa
    29221 actttattcc cttctttttt agcaactcct tcttcgggtt taaacagttc atagttactg
    29281 tgagaatgac caattccaac cggtttatgt tcatcaatcg gatctgcatg atggtgatct
    29341 ccatgcggat aaataatcgc atttttttct ttattcacga caatactttc acgtttgaca
    29401 ccatattgtt tcataatgcc agcaattttt tcttcgattt ttttatctaa atctttcatt
    29461 tctttggcat tacttggata atcctgttca tgagatgaca aagaatctaa tccattatga
    29521 ctagttttaa cttcctctaa atgtttttgc gcagccttaa tttgctcttc tgtcaagtcc
    29581 ttcttgaaga aataatgatt gtggtctccg tgactcatga caaaacctga ttcatcttca
    29641 gcgataatac gattagcatc aaatccgtat ccatcttctt catgtttctc atgtgaagtt
    29701 cctggattga ttggaagaga tggagaaggt gttgctagac tattgtttgg aagagtcggt
    29761 tgcccaattt gatttgattt tggaatgtaa tggaaatgat caccatgtct tacaatataa
    29821 gctgtagccg tttcttcaac gatatctttt ggattaaaaa tataaccatc agatgctgaa
    29881 gagagctcct tacttgtcgt taaagaagaa ggattgcttg aaagactgcc tagactagac
    29941 actacttcat taggttttgc atttgtagaa actgtagaac cagttccact gataggcacc
    30001 attctggcaa tcttttcttc taaggcagaa agcttgctgt aaggaataaa gtggtaatgg
    30061 tcgccatgcg gaatcgcaac tccatttggt gtacgactga taatcttagc agggtcaaag
    30121 accaggccat ctgattcact gtaacgttgg gcgctaggtg aatcatagag ttccttcaaa
    30181 agactctgga gattttcaga tttatttgct ggcttgctag ttgatccttt tgctacagat
    30241 tgcgtgttat tgtcactagc tgttgaagaa tagcttaact gactcggttg catatttttt
    30301 ccagccagat gtgctttagc tgctgctaat tcactagcag ataaatcgct tttgggaatg
    30361 tagtgatagt gacctccatg aggaacgata taagcattac ccgtatcttc gataatatca
    30421 gctggattaa agacataacc atcatttgtc gtatatcgtc cctgagacct tgctacagca
    30481 acattagagt taaccttctc attatctttg acatgttctt gtttttgacg attgatttca
    30541 tctttagttc gaacattatc agcatgagct gcatctttca ggtagacata atattttcca
    30601 tcgaccttga tgatataacc acccttgact tcattgacaa tatcagcgtc tttaagttga
    30661 tagtttggat ccttcatcaa gagttcttca ctaaagaggg catcataagg aactttccca
    30721 ttatagtaat gatagtggtc accgtgtgac gttacatagc cctgatctgt aattttgatt
    30781 acaatttgct cggcctgaat tccttctttc tggctaatct ggtctggtgt caagttttca
    30841 cttttctgac ttgactggct gccatctaca taagagacac gattattgtc cttattttcc
    30901 tgcgaacgat gctggtttag tgcataggca catagactca aggatacgat aacagctgat
    30961 ccagctgcta tatatttttt actgaatttc ataaatccct ca
//
LOCUS       DAVBBS010000020        30969 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVBBS010000020 DAVBBS010000000
VERSION     DAVBBS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30969
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.020"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..188)
                     /locus_tag="VQU32_000896"
     CDS             complement(<1..188)
                     /locus_tag="VQU32_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET2472369.1"
                     /translation="MNYEASKQLTDARFKRLVGVQRTTFEEILAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVR"
     gene            183..437
                     /locus_tag="VQU32_000897"
     CDS             183..437
                     /locus_tag="VQU32_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET2472370.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIGAEAIRRNCERGAIFSGKIKHHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            781..2115
                     /gene="trmFO"
                     /locus_tag="VQU32_000898"
     CDS             781..2115
                     /gene="trmFO"
                     /locus_tag="VQU32_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET2472371.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRIFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEAIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            2199..2936
                     /locus_tag="VQU32_000899"
     CDS             2199..2936
                     /locus_tag="VQU32_000899"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET2472372.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            2945..3502
                     /gene="frr"
                     /locus_tag="VQU32_000900"
     CDS             2945..3502
                     /gene="frr"
                     /locus_tag="VQU32_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET2472373.1"
                     /translation="MANAIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRIHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVV
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3562..4416
                     /gene="cvfB"
                     /locus_tag="VQU32_000901"
     CDS             3562..4416
                     /gene="cvfB"
                     /locus_tag="VQU32_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HET2472374.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTSLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4425..4640
                     /locus_tag="VQU32_000902"
     CDS             4425..4640
                     /locus_tag="VQU32_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HET2472375.1"
                     /translation="MRKSFYTWLMTERNPKSNNPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            4726..5694
                     /locus_tag="VQU32_000903"
     CDS             4726..5694
                     /locus_tag="VQU32_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET2472376.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMALVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            5874..6374
                     /locus_tag="VQU32_000904"
     CDS             5874..6374
                     /locus_tag="VQU32_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2472377.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            6377..6637
                     /locus_tag="VQU32_000905"
                     /pseudo
     CDS             6377..6637
                     /locus_tag="VQU32_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(7004..8115)
                     /gene="ald"
                     /locus_tag="VQU32_000906"
                     /pseudo
     CDS             complement(7004..8115)
                     /gene="ald"
                     /locus_tag="VQU32_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253293.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            8292..8861
                     /locus_tag="VQU32_000907"
     CDS             8292..8861
                     /locus_tag="VQU32_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2472378.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            8929..9579
                     /locus_tag="VQU32_000908"
     CDS             8929..9579
                     /locus_tag="VQU32_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET2472379.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTSVKETNLQ
                     AEVAAVSKDSSTGKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            9563..11803
                     /locus_tag="VQU32_000909"
     CDS             9563..11803
                     /locus_tag="VQU32_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HET2472380.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSVSYFALLGFVFLLVCL
                     FIQFPWKSAGKVLVICGVFGFWFLFQTWQQSQVSQNLVDSVERVRILPDTIKVNGDSL
                     SFRGKSDGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFDYQGY
                     LKTQGIYQTLNIKRIQSLQKVGSWDIGENLSSLRRKAVVWIKMHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMDGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     VFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVASRPLVFGQPNEWLLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIKKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEMTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMIVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            11983..12171
                     /locus_tag="VQU32_000910"
     CDS             11983..12171
                     /locus_tag="VQU32_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472381.1"
                     /translation="MTVTKSYKYDWNTVLEYSTNYHDHQYAWIPEWSRYYSYSEYKVG
                     GGWNYARYEVINYYTGGY"
     gene            12203..12790
                     /locus_tag="VQU32_000911"
     CDS             12203..12790
                     /locus_tag="VQU32_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2472382.1"
                     /translation="MLNLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            12794..13969
                     /locus_tag="VQU32_000912"
     CDS             12794..13969
                     /locus_tag="VQU32_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472383.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWKDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLPKQIDNVDLNWSMGMVLLPCLIIFLLLGILFIERWGSSQKKRIF"
     misc_feature    14143..14274
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14290..14820
                     /gene="infC"
                     /locus_tag="VQU32_000913"
     CDS             14290..14820
                     /gene="infC"
                     /locus_tag="VQU32_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET2472384.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            14853..15053
                     /gene="rpmI"
                     /locus_tag="VQU32_000914"
     CDS             14853..15053
                     /gene="rpmI"
                     /locus_tag="VQU32_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET2472385.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            15105..15464
                     /gene="rplT"
                     /locus_tag="VQU32_000915"
     CDS             15105..15464
                     /gene="rplT"
                     /locus_tag="VQU32_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET2472386.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            15522..15902
                     /locus_tag="VQU32_000916"
     CDS             15522..15902
                     /locus_tag="VQU32_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2472387.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            16152..16952
                     /locus_tag="VQU32_000917"
     CDS             16152..16952
                     /locus_tag="VQU32_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HET2472388.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVNQRVCEDGPVFRTGTVVL"
     gene            16915..17901
                     /locus_tag="VQU32_000918"
     CDS             16915..17901
                     /locus_tag="VQU32_000918"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HET2472389.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            17943..19844
                     /locus_tag="VQU32_000919"
     CDS             17943..19844
                     /locus_tag="VQU32_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HET2472390.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYM
                     AKNETVDGYQLGSDGKWLGGKATNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWL
                     DKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIP
                     VASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINN
                     SLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYL
                     SAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMK
                     INEKLGGKD"
     gene            complement(19905..21560)
                     /gene="pavA"
                     /locus_tag="VQU32_000920"
     CDS             complement(19905..21560)
                     /gene="pavA"
                     /locus_tag="VQU32_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HET2472391.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPLANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            21665..22162
                     /gene="ybeY"
                     /locus_tag="VQU32_000921"
     CDS             21665..22162
                     /gene="ybeY"
                     /locus_tag="VQU32_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET2472392.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLGYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            22143..22538
                     /locus_tag="VQU32_000922"
     CDS             22143..22538
                     /locus_tag="VQU32_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HET2472393.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFGFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            22555..23454
                     /gene="era"
                     /locus_tag="VQU32_000923"
     CDS             22555..23454
                     /gene="era"
                     /locus_tag="VQU32_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET2472394.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPDRFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            23503..24327
                     /gene="mutM"
                     /locus_tag="VQU32_000924"
     CDS             23503..24327
                     /gene="mutM"
                     /locus_tag="VQU32_000924"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HET2472395.1"
                     /translation="MPELPEVETVCRCLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARSSQTLTAEEATAIHDQTIAVLSQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQGCVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            24327..24932
                     /locus_tag="VQU32_000925"
     CDS             24327..24932
                     /locus_tag="VQU32_000925"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET2472396.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFWQEIILENGELNRPLLASLIFSNPDEREWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            24919..26118
                     /locus_tag="VQU32_000926"
     CDS             24919..26118
                     /locus_tag="VQU32_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HET2472397.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            26118..26267
                     /gene="rpmG"
                     /locus_tag="VQU32_000927"
     CDS             26118..26267
                     /gene="rpmG"
                     /locus_tag="VQU32_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2472398.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            26307..26540
                     /gene="secG"
                     /locus_tag="VQU32_000928"
     CDS             26307..26540
                     /gene="secG"
                     /locus_tag="VQU32_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET2472399.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            26642..28996
                     /gene="rnr"
                     /locus_tag="VQU32_000929"
     CDS             26642..28996
                     /gene="rnr"
                     /locus_tag="VQU32_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HET2472400.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSHSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            28959..29426
                     /gene="smpB"
                     /locus_tag="VQU32_000930"
     CDS             28959..29426
                     /gene="smpB"
                     /locus_tag="VQU32_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET2472401.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            29442..30302
                     /gene="tehB"
                     /locus_tag="VQU32_000931"
     CDS             29442..30302
                     /gene="tehB"
                     /locus_tag="VQU32_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HET2472402.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEDVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            30375..>30969
                     /gene="coiA"
                     /locus_tag="VQU32_000932"
     CDS             30375..>30969
                     /gene="coiA"
                     /locus_tag="VQU32_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HET2472403.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQG"
BASE COUNT         9195 a         5732 c         7093 g         8949 t
ORIGIN      
        1 cgcacatatt gaagagtggc cataagaagg tcttctaggc ttaatttagg ttttcgtcca
       61 ccttttgcgt gtttaagttg ataagctgtt tttaatacag ctaatatctc ttcaaaagtc
      121 gtgcgctgaa caccaacaag acgcttaaat cgtgcatcag ttagttgttt acttgcttca
      181 taattcatag aactactata ccatattttg tttcgcagga agtctattgg aaagtaagaa
      241 atattggagc tgaggcgatt agaagaaatt gtgagcgtgg tgctattttt tcaggtaaaa
      301 taaaacatca cgaagattct cagtttaaag gagaccacta tgttgaatgt tatgctgttt
      361 tagataatac ggttatagca agagatagaa taacagtccc tatcgatccg ttatgtggaa
      421 aagattttat agagtagcat ataattgatt cttaactgga atactctcta tctctttaca
      481 tcaagaaaat gactaaacag ggaagtttgc cttcttccct ttttttgtta tactagtaga
      541 agaaaaaaat agaaagattt gtgggagtga aaaagtccgg gggacttttt cagcttgagc
      601 caagaaattc gagagcgagg aaagtccggg ggactttttc agcctgagcc aagaaattcg
      661 agagcgaagt aagtccaatg gactttttca gcctgagcca agaaactcga aagctcgtaa
      721 gtctgattgg ctttttcaat gtgaatctta acttcacact cccaaagagg tattagtgtc
      781 gtgtctcaat cttatatcaa tgttatcggt gctggtttgg caggttctga agcagcttac
      841 caaatcgcag agcgtggtat tccagttaaa ctatatgaaa tgcgtggtgt caagtcgact
      901 ccccagcata aaacagacaa ttttgctgag ttggtttgtt ccaattcttt gcgtggggat
      961 gctttgacaa atgcagttgg tcttctcaag gaagaaatgc gtcgcttggg ttctgttatc
     1021 ttggaatctg ctgaggctac acgtgttcct gcaggtggtg cccttgcagt ggaccgtgat
     1081 ggtttctctc aaatggtgac cgaaaaagtt gccaaccacc ccttgattga agtggttcgt
     1141 gatgaaatta cagaattgcc gacagatgtt attacggtta tcgctactgg tcctttgaca
     1201 agtgatgcct tggctgaaaa gattcatgcc cttaatgacg gtgatggttt ttatttctac
     1261 gatgcggcag cgcctattat cgatgtcaac actatcgata tgagcaaggt ctacctcaaa
     1321 tcacgttatg ataagggaga agcggcctac ctcaatgccc ctatgaccaa gcaagaattt
     1381 atggatttcc atgaagcttt ggtcaatgca gaagaagcac cgcttaattc ttttgaaaaa
     1441 gaaaagtact ttgaaggctg tatgcctatc gaagtcatgg ccaaacgtgg cattaaaact
     1501 atgctttatg gccctatgaa gccagtcggt cttgagtacc cagacgacta tacaggacct
     1561 cgtgatggag aatttaaaac accttatgcg gttgtgcaac ttcgtcagga caatgcagct
     1621 ggtagcctct acaatattgt tggtttccag acccacctca aatggggaga acaaaagcgt
     1681 atcttccaaa tgattccggg tcttgaaaat gcagagtttg tccgttatgg tgtcatgcat
     1741 cgcaattctt acatggattc accaaatctt cttgagcaga cttaccgttc taagaaacaa
     1801 ccaaatctct tctttgctgg tcagatgacg ggggtggaag gctatgttga gtcggcggct
     1861 tcaggcttag ttgcgggaat taacgcagct cgtctcttca aggaagaaag cgaggctatt
     1921 ttccccgaga cgacagcgat tggaagctta gctcattaca ttacccatgc cgacagcaaa
     1981 catttccaac caatgaatgt caattttggg atcatcaagg agttggaagg cgagcgtatc
     2041 cgtgataaga aggctcgtta tgaaaaaatt gcagagcgtg cccttgccga cttagaggaa
     2101 tttttgactg tctaattttt ttgaaagaat tgctcatgat actataaaaa tcttagaaat
     2161 tgtgataaaa taggtaggat gaaagaagga gagtgaaaat ggcgaatccc aagtataaac
     2221 gtattttaat caagttatca ggtgaagccc ttgccggtga acgtggcgta gggattgata
     2281 tccaaacagt tcaaacaatc gcaaaagaga ttcaagaagt tcatagctta ggtatcgaaa
     2341 ttgcccttgt tattggtgga ggaaatctct ggcgtggaga acctgcagca gaagcaggta
     2401 tggaccgtgt tcaggcagat tacactggaa tgcttgggac tgttatgaat gctcttgtga
     2461 tggcagattc attgcaacaa gttggggttg atacgcgtgt acaaacagct attgctatgc
     2521 aacaagtggc agagccttat gtccgtggac gtgcccttcg tcaccttgaa aaaggccgta
     2581 tcgttatctt tggtgctgga attggttcac catacttctc gacagataca acagcggccc
     2641 ttcgtgcagc tgaaatcgaa gcggatgcca tcctcatggc taaaaatggt gtcgatggtg
     2701 tttacaatgc cgatcctaag aaagataaga cagctgttaa gtttgaagaa ttgacccacc
     2761 gtgacgttat caataaaggt cttcgtatca tggactcaac agcttcaacc ctctcaatgg
     2821 acaacgacat tgacttggtt gtcttcaaca tgaaccaatc aggcaacatc aaacgtgtcg
     2881 tatttggtga aaatatcgga acaacagttt caaataatat cgaagaaaag gaataagaaa
     2941 gaatatggct aacgcaatta ttgaaaaagc taaagagaga atgacccagt ctcaccaatc
     3001 acttgctcgt gaatttggtg gtatccgtgc tggtcgtgcc aatgcaagct tgcttgaccg
     3061 tatacatgta gaatactatg gagttgaaac tcctcttaac caaatcgctt caattacgat
     3121 tccagaagcg cgtgttttgt tggtaacacc atttgacaag tcttcattga aagacatcga
     3181 acgtgccttg aacgcttctg atcttggtat cacaccagct aatgacggtt ctgtgattcg
     3241 cttggttgtc ccagctctta cagaagagac tcgtcgtgac cttgctaaag aagtgaagaa
     3301 ggtcggcgaa aatgctaaag tggctgtccg caatatccgt cgcgatgcta tggacgaagc
     3361 taagaaacaa gaaaaagcaa aagaaatcac tgaagacgaa ttgaagactc ttgaaaaaga
     3421 cattcaaaaa gtaacagacg atgctgttaa acacatcgac gatatgactg ccaacaaaga
     3481 gaaagaactt ttggaagtct aaaaataaac agaaaaactc agttggcatt gctggctgag
     3541 ttttattcga aagaaggaaa tatgaataca aatcttgcaa gttttatcgt tggactgatc
     3601 atcgatgaaa acgaccgttt ttactttgtg caaaaggatg gtcaaaccta tgctcttgct
     3661 aaggaagaag gccaacatac agtaggggat acggtcaaag gttttgcata cacggatatg
     3721 aagcaaaaac tccgcctgac aagcttagaa gtgactgcca ctcaggacca atttggttgg
     3781 ggacgtgtca cagaggttcg taaggacttg ggtgtctttg tggatacagg ccttcctgac
     3841 aaggaaatcg ttgtgtcact cgatattctc cctgagctca aggaactctg gcctaagaag
     3901 ggcgaccaac tctacatccg tcttgaagtg gataagaaag accgtatctg gggtctcttg
     3961 gcttatcaag aagacttcca acgtcttgct cgtcctgcct acaacaacat gcagaaccaa
     4021 aactggccag ccattgttta ccgtctcaag ctatcaggaa cttttgttta cctaccagaa
     4081 aataatatgc ttggttttat tcatcctagc gagcgttacg cagagccacg tttggggcaa
     4141 gtattagatg cgcgcgttat tggtttccgt gaagtggacc gcactttgaa cctctccctc
     4201 aaaccacgct cctttgaaat gttggaaaac gatgctcaga tgattttgac ttatttggaa
     4261 agcaatggcg gtttcatgac cttaaatgac aagtcatctc cagacgacat caaggcaacc
     4321 tttggcattt ctaaaggtca gttcaagaaa gctttaggtg gtcttatgaa ggctggtaaa
     4381 atcaagcagg accagtttgg tacagagttg atttagggag gcttatgaga aaatcatttt
     4441 acacttggct catgaccgag cgcaatccta aaagtaacaa tcccaaagca attttggcag
     4501 acctcgcttt tgaagaggca gcttttccaa aacatacaga tgattttgat gaggtcagtc
     4561 gctttttgga ggagcatgcc agtttctctt ttaacctagg agattttgac agcatttggc
     4621 aggaatatct agaacactag catttattca ttgggtttgg gctagtaatt tctccatccc
     4681 tctgctataa taaaaagaaa taaaaggatt agagaggttc tttatttgaa ggaacattca
     4741 atagacattc aactgagtca tccagatgac ctgtttcatc tttttggttc caatgaacgc
     4801 catcttcgtt tgatggaaga agagcttgat gttgtgattc atgctcgtac ggagattgtc
     4861 caggttttgg gagaagagtc tgcttgcgag gaagcccgtc aggttattca agctctcatg
     4921 gccttggtaa atcgtgggat gaccgttggt acgccagatg tagtgacggc tattagtatg
     4981 gtcaaaaatg atgaaattga caagtttgtc gccctttacg aagaagaaat catcaaggat
     5041 aatactggga agcctatccg cgtcaaaacc ttaggtcaaa aactttatgt ggacagtgtc
     5101 aaacagcatg atgtgacctt tggaattggg ccagcaggta cagggaagac cttccttgca
     5161 gtgaccttgg cagtgactgc ccttaaacgt gggcaagtca agcgaattat cctaactcgt
     5221 ccagcggtgg aagcgggaga gagtcttgga tttcttccgg gtgatcttaa ggagaaggtg
     5281 gatccttacc ttcgtcctgt ttacgatgcc ttgtatcaaa ttcttgggaa agaccaaacg
     5341 actcgtctca tggagcgtga aattatcgaa attgcgcccc ttgcctatat gcgtggccgg
     5401 accttggatg atgcctttgt cattctcgat gaggcgcaaa acacgaccat catgcagatg
     5461 aagatgttct tgacgcgttt aggctttcat tctaagatga ttgtcaatgg agatatcagt
     5521 cagattgacc tgccacgtaa tgtcaagtcc ggtttgattg atgctcaaga gaaactcaag
     5581 aacatccatc agattgactt tgttcatttt tcagccaagg atgtggttcg ccatccagtt
     5641 gtcgctcaga ttatccgagc ctatgaatat tctactgaag ttgcacacga ctgattttga
     5701 ggaagttcgc ctgcaaaaga atatactttt gagagaggaa aatccagttg tataggctaa
     5761 aggtttgaga caacgattag gcacgatgga aagaactttt atgtggctga tgacgatcag
     5821 tgcatcttcc tgtgtcataa tcacagggca caagaaagta ggaatttgaa aagatgattg
     5881 accaactatc taagtattac agttgtagga tactaactga aaaggatatt ccaagtattt
     5941 tatctttata tgaaagtaat cctctgtatt ttcagcattg tccaccagag ccaaattttg
     6001 caactgtaaa agaggacatg ctttgtctac ctgaaggtaa agctaaggct gataagtttt
     6061 ttgttggatt ttggaatggc tctgaccttg tggctgttat ggattttgtc tatgcatatc
     6121 ctgatgagga gactgttttt attggtttgt ttatggttga tcaagcctat cagaggaaag
     6181 ggattggtag tcatattgtg acagaagcac tagcttattt tgctaagaac tttcgaaagg
     6241 cacgtttggc ttatgttaag ggaaatccgc aatctcagca tttttgggaa aagcagggct
     6301 ttaaatcaat tggatgcgag gttaagcaag aactctatac ggttgttatc gctgaacaga
     6361 gcctagaaga ttagaaatgg catcaagtaa gaactatttg gaatttgttt tggaacaatt
     6421 atcaggatta gatgatgtga cttaccgttc catgattggg gagtatattc tttacttccg
     6481 cggcaagatt attggcggca tttatgacga tcgcttttta gttaaacccg tgcaagcagt
     6541 cttagataag attgaccaat cttcttttga gtttccatac aaaggtgcca aagaaatgat
     6601 ttgagtggaa gaacttgata ataagatgtt tctataagac ctgattttag ctatgtataa
     6661 ccaactgcca acgcccaaac ctaaaaagaa aaagcaaggg tgaacgaagt aaaaaagaag
     6721 tctgctaagg ccctgtcttt gcacgggtaa aattttatat ataaaaagaa gctgggacta
     6781 aagagctcag cttcctttgg tttatataat tgtcattaca agacgaagtg gttgggcgaa
     6841 actctgttga ctttattcaa tttagagttt cttatgcaca attgagtctg gaacgaaagt
     6901 ctccagttgc aaagtataca gtacaataaa ccaacgatgt aatagctgat gacacaaagc
     6961 acagtgggta ggacttgcga agtcaccctt ttcttttcaa aatttatact aaatcattga
     7021 tatcagtgta gtcacgatta agtccttgag caactggtag gctagtcaag taaccttgat
     7081 aagtggtcac accttgacgc aagccttcat cttcagagat tgcttgtgcg aatcctttgc
     7141 cagccaaagc ttcgatataa ggaagagtga cattggttag ggcgatggtt gaagtgcggg
     7201 caaccgcacc agggatattg gcaacggcat agtggagaac accgtgtttt tcatagacgg
     7261 gttcatcgtg cgttgtcaca cggtcagctg tttcgataac gccaccttgg tcaacagcaa
     7321 cgtcaacgat acagagcctg gacgcatttg tttgaccatc tcatctgtca ccaattccgg
     7381 tgcttttgca ccagggatga gaatggctcc aatcaccaca tcagcatctc tcacacttgc
     7441 ttcaatgttg aatgaattag atataatagt ttgaatttga cttccaaaga cttcttctag
     7501 aactgagaga cgcttggcac taatatctaa aatagtcact tgagcaccaa gaccaagggc
     7561 gatgcgggca gcatgtgtac cgacgacacc atcaccgatg atagttactt ttccttttgg
     7621 aacacctggt acaccaccaa gtagaacacc agagccacca gcttgcttag taaggaagtg
     7681 agctccgatt tgaacagcca tacgacctgc aacctcactc ataggaacga ggagcggtag
     7741 ttgtccttga ttgtcacgaa cagtttcata ggcaattcct gttgtttttg ctgctaacat
     7801 agcatctgct aattctggag cagcggccat gtgcaagtag gtgaagagaa gaagatcgtc
     7861 gcgcaagtaa ccgtattcag aacttaaagg ttcttttact ttcacaacca actctgctgc
     7921 ccaagcttca ccagcagtag cgacaatctc agctccttgc ttttgatagt cagcatcagt
     7981 aaagccagaa ccgagaccag catttgtttc gataaggaca cgatgaccac gactaactaa
     8041 gctatgaaca cctgcaggtg tgagggcgac acggttttcg ttatttttaa tttcttttgg
     8101 gattccgatt aacattgaga taacctacct ttcaattgac ggtcttgttt tggttgtcac
     8161 attccagttc ataaatcaaa aatgtgacgg tttcattgta tatgaaaccg cttcaaaaat
     8221 caagaaaaac ttgtcatcca aattttttta tgctagacta gtgaaaatca agctctaatg
     8281 gagggaaaag tatggaatca atatttgtga aatttgccca gtatccgtct atagaaacgg
     8341 agcgtttatt gcttagacct gtaactttgg atgatgcgga agcaatgttt gactatgcct
     8401 cggacaaggg taatacacgt tacacttttc caaccaatca aagcttggaa gaaaccaaga
     8461 ataacattgc tcagttctac ttggctaatc ccttgggacg ttgggggatt gaattaaaag
     8521 ataacggcaa gtttatcgga actattgact tacacaagat tgatcctgtt cttaagaagg
     8581 cagctattgg ctacattatc aataaaaagt attggaatca aggattaacg acagaagcca
     8641 atcgtgctgt gattgagcta gcttttgaga agatagggat gaataagttg actgcccttc
     8701 acgataaggc taatcccgcg tcaggaaagg tcatggagaa atcaggcatg cgtttttccc
     8761 atgcagaacc atatgcttgt atggaccagc atgaaaaagg ccgaatcgtg acaagagttc
     8821 attatgtctt gaccaaggaa gactattttg caaataaata agcagttgaa aagaaatttt
     8881 tcgactgttt tttcttcctc ttacgaataa tctaagagag gagaaaatat ggaagcaatt
     8941 atcgagaaaa tcaaagagta taaaatcatc gtcatctgta ctggtctggg cttgcttgta
     9001 ggaggatttt tcctgctaaa gccagctcca caaacatctg tcaaagagac gaatttgcag
     9061 gctgaagttg cagctgtttc caaggactca tcgaccggaa aggaagtgaa caaggaagag
     9121 aaggaagaac cagttgaaca agatctaatc acagtagatg tcaaaggtgc tgtcaaatcg
     9181 ccagggattt atgacttgcc tgtaggtagt cgagtcaatg atgctgttca gaaggctggt
     9241 ggcttgacag agcaagcaga cagcaagtcg ctcaatctag ctcagaaagt tagtgatgag
     9301 gctctggttt acgttcctac taagggagaa gaagcagtta gtcaacagac tggttcgggg
     9361 acagcttctt caacaagcaa ggaaaagaag gtcaatctca acaaggccag tctggaagaa
     9421 ctcaagcagg tcaagggact gggaggaaaa cgagctcagg acattattga ccatcgtgag
     9481 gcaaatggca agttcaagtc agtagacgag ctcaagaagg tctctggcat tggtgccaag
     9541 accatagaaa aacttaaaga ctatgttaca gtggattaag aatttctcta ttcccctaat
     9601 ttacctgagt tttctattac tatggcttta ctacgccatt ttctcagtat cctattttgc
     9661 tttgttgggt tttgtttttc tgctagtctg cctctttatc caatttcctt ggaaatcagc
     9721 aggtaaagtt ctagtgattt gtggagtctt tggcttctgg tttctgtttc aaacttggca
     9781 acagagtcaa gtgagtcaaa atttggtgga ttctgttgaa agggtacgga ttttaccaga
     9841 cactattaag gttaacggtg acagtctgtc ctttcgtggt aagtctgatg gtcgtgcttt
     9901 ccaagtctat tataaactcc agtccgagga ggagaaagaa gcctttcaag ctttaactga
     9961 ccttcatgag ataggactag aagggaagct ttcggagcca gaagggcaga gaaattttgg
    10021 tggctttgac taccaaggct atctgaagac tcagggaatt taccagacac tcaatattaa
    10081 aagaatccag tcactccaaa aggttggcag ttgggatata ggtgaaaacc tgtccagttt
    10141 acgtcgaaag gctgtggttt ggattaagat gcacttccca gaccctatgc gcaattacat
    10201 gacaggactc ttgctaggac atctggacac cgattttgag gagatgaatg agctttattc
    10261 cagtttagga attattcacc tttttgcctt gtcaggtatg caggtagggt ttttcatgga
    10321 cggatttaag aaactacttt tacgattggg cttgacacaa gaaaagttga agtggctgac
    10381 ttatcccttt tcccttatct atgcgggact aactggattt tcagcatcgg ttattcgcag
    10441 tctcttgcaa aagctactgg ctcaacatgg ggttaagggc ttggataatt ttgccttgac
    10501 ggtgcttgtc ctctttattg tcatgccaaa ctttttcttg acagcaggag gagtcttgtc
    10561 ctgtgcttat gcttttatcc tgaccatgac cagcaaagaa ggggaggggc tcaaggctgt
    10621 tactagtgaa agtctagtca tctccttggg catattgccc attctatcct tctattttgc
    10681 ggaatttcaa ccttggtcta tccttttgac ctttgtcttt tcctttcttt ttgacttggt
    10741 cttcttaccg ctcttgtcta tcttatttgt cctttccttt ctctatccag tcattcagct
    10801 gaactttatc tttgaatggt tagagggcat tattcgcttg gtctcgcagg tggcaagtag
    10861 accgcttgtc tttggacaac ccaatgaatg gcttttaatc ctattgttaa tttctttggc
    10921 tttggtctat gatttgagga aaaacattaa aaagctaacg gtattgtgct tattgattac
    10981 agggctcttt ctcctgacca agcatccact ggaaaatgaa atcaccatgc tggatgtggg
    11041 gcaaggagaa agtattttcc tacgggatgt aactgggaaa accattctca tagatgtagg
    11101 tggtaaggca gaatcttata agaaaatcaa aaaatggcaa gaaaagatga cgaccagcaa
    11161 tgcccagcga agcttgatac cctatctcaa aagtcgagga gtagctaaga ttgaccagct
    11221 aattttgact aacacggaca aggagcatgt tggagatttg tcagagatga ccaaggcttt
    11281 ccatgtaggg gagattctag tatcaaaaga cagtctgaaa cagaaggaat ttgtggcaga
    11341 actacaggcg actcaaacaa aggtgcgtag tatgatagta ggggagaact tacccatttt
    11401 tggaagtcag ttagaagttc tatctccaag gaaaatggga gatggaggac acgatgatac
    11461 cctagttctg tatgggaaat tcttggataa gcaatttctc ttcacgggaa atttggagga
    11521 gaaaggagag aaggacttgc tgaagcacta tccagacttg aaagtaaatg ttttgaaagc
    11581 tagccaacat ggcaataaaa aatcatcaag tccagccttt ctagaaaaac tcaaaccaga
    11641 gcttactctt atctcagttg gaaagagcaa tcgaatgaaa ctcccccatc aggaaacatt
    11701 gacacgactg gaaggtatca atagcaaagt ttatcgaact gaccagcaag gagctatacg
    11761 ttttaagggg ttggatagtt ggaaaatcga aagtgttcga taggaaggat aaatgttgta
    11821 gattagtgaa ataaactaaa aatttgttgc ataataatga taaaaacggc ataatgaaaa
    11881 cgtattcaat attgaggata taaaatcatt aaaaatcagc aaaagttgtt ttagttagtt
    11941 agtttataat ctattggtct tcttcagtcc agtgtatctg ctgtgacagt cactaaaagt
    12001 tacaagtatg attggaatac ggttttggaa tatagtacca actatcacga ccatcagtat
    12061 gcttggattc cggaatggtc tcgttattac agctattctg agtataaagt tggcggaggc
    12121 tggaactacg ctcgttatga ggtcataaac tactatactg gaggttatta atccttaaag
    12181 agtgagaaaa ggagggctgg atatgttaaa tcttactcat gttaccttaa aaacgcgaca
    12241 agtcatcttg caagatgcgg attttacctt taaaaagggt aggatttatg gccttcttgc
    12301 tatcaatggc tcgggaaaga cgacactatt ccgagctatg agcaagttgc ttccccttag
    12361 tagtggacac atcgcagttc ctccttcttt gttttattat gagagcgttg aatggctgga
    12421 tggaaactta agtgggatgg actaccttcg tctcataaaa aacatctgga agtcagacct
    12481 aaacttgaga gatgaaatcg cctactggga aatggctgac tatatcagtc ttcccatccg
    12541 caagtattcc ttagggatga agcaacgctt ggtgattgct atgtattttc tcagtcaggc
    12601 caaatgctgg ctcatggatg agattacaaa tggcttagac gagtattatc gacagaagtt
    12661 ttttgatagg ctagcacaaa tcgatagaca agaacagctg gttcttttaa gttcccacta
    12721 taaggaagag ttggttgata tctgcgatag agtagtaacc attcatcagg ggcagataga
    12781 agaggtttag tttatgaaag atgttagtct atttttattg aaaaaagttt tcaaaagtcg
    12841 tttaaactgg attatcttag ctttatttgt atctgtactc ggtgttacct tttatttaaa
    12901 tagtcagact gcaaactcac acagcttgga gagcaggttg gaaagtcgca ttgcagccaa
    12961 cgagagggct atcaatgaaa atgaagagaa actctcccaa atgtctgata ccagctcgga
    13021 ggaataccag tttgctaaaa ataatttaga cgtgcaaaaa aatcttttga cgcgaaagac
    13081 agaaattcta actttactaa aagaagggcg ctggaaagaa gcctactatc tgcagtggaa
    13141 agatgaagag aagaattatg aatttgtatc aaatgacccg actgctagct ctggcttaaa
    13201 aatgggggtt gaccgcgaac ggaagattta ccaagccctg tatcccttga acataaaagc
    13261 acatactttg gagtttccga cccacgggat tgatcagatt gtctggattt tagaggttat
    13321 catcccaagc ttgtttgtgg ttgcgattat ttttatgcta acacaactat ttgcagaaag
    13381 atatcaaaat catctggaca cagctcactt atatcctgtt tcaaaagtga catttgcaat
    13441 atcctctctt ggagttggag tgggatatgt aactgtgctg tttatcggaa tctgtggctt
    13501 ttcttttcta gtgggaagtc tgataagtgg ttttggacag ttagattatc cctacccaat
    13561 ttatagctta gtgaatcaag aagtaactat tgggaaaata caagatgtat tatttcctgg
    13621 cttgctctta gctttcttag cctttatcgt cattgtggaa gttgtgtact tgattgctta
    13681 ctttttcaag caaaaaatgc ctgtcctctt tctttcactc attgggattg ttggcttatt
    13741 gtttggcatc caaacaattc agcctcttca aaggattgca catctgattc cctttactta
    13801 cttgcgttca gtggagattt tatctggaag attacctaag cagattgata atgtcgatct
    13861 aaattggagc atgggaatgg tcttacttcc ttgcctgatt atctttttgc tattgggaat
    13921 tctatttatt gaaagatggg gaagttcaca gaaaaaaaga attttttaat agattctagc
    13981 tttcctatag gtagggaaaa taagtaaaaa ctaacataga gagggaatca acttgattct
    14041 ctctttttga ttcgaaaacc aaaccaaaat acaaacacaa acttttcaaa aaataacttt
    14101 ttatcttgac aagagctaga aaacttggta tcatataaaa gttgagaaaa gcagaagtga
    14161 gagcttctcg ccttgtgaca ttaagttgcc tggccctacg gatgaaaagt ttcgaagaaa
    14221 cgctatcata acgtgcgggc ttgtatattt acgagtccgc tattgttttt ctctaataaa
    14281 acaaaagagg tgaaaaccat agcaaagcaa gacttattca tcaatgatga gattcgtgta
    14341 cgtgaagttc gcttgattgg tcttgaagga gaacagctag gcatcaagcc actcagtgaa
    14401 gcgcaagctt tggctgataa cgctaatgtt gacctagtat tgattcaacc ccaagccaaa
    14461 ccgcctgttg caaaaattat ggactacggt aagttcaaat ttgagtacca gaagaagcaa
    14521 aaagaacaac gtaaaaaaca aagcgttgtt actgtgaaag aagttcgtct aagtccaact
    14581 attgacaagg gtgactttga tacaaaactt cgtaatgcac gtaagttcct tgaaaaagga
    14641 aataaagtta aggtatctat tcgctttaag ggtcgtatga ttacccataa agagattggt
    14701 gcaaaagttt tagccgagtt tgctgaagca actcaagata ttgccatcat cgaacaacgt
    14761 gctaaaatgg atggacgtca aatgttcatg caattggcgc cagcgactga caaaaaataa
    14821 ttgtcagaaa gttaaataaa ggagaaaaaa ttatgccaaa acaaaaaaca caccgcgcat
    14881 cagctaaacg tttcaaacgt acaggttctg gtggacttaa acgtttccgt gcttacactt
    14941 ctcaccgttt ccacggaaaa actaagaaac aacgtcgtca tcttcgtaaa gcatctatgg
    15001 tgcattcagg agattacaaa cgtatcaaag caatgcttac tcgcttgaaa taatagcttg
    15061 actgtaagta aacaattcta ggaaatattt ggaggaaata aaacatggca cgtgttaaag
    15121 gtggcgttgt atcacgcaaa cgtcgtaaac gtattcttaa attagcaaaa ggttactatg
    15181 gagctaaaca catcttgttc cgtactgcaa aagaacaagt aatgaactct tactactatg
    15241 cataccgtga ccgtcgtcag aaaaaacgtg acttccgcaa attgtggatc actcgtatca
    15301 atgcggcagc tcgtatgaac ggactttcat actcacaatt gatgcatggt ttgaaattgg
    15361 ctgagatcga agttaaccgt aaaatgcttg ctgacttggc tgttaacgat gcagtagctt
    15421 tcacagctct tgcagatgca gctaaagcaa aacttggtaa ataacaactg atgagactgg
    15481 aacaggattg tccagtcttt ttaaaaataa aggagaaaaa tatggcttca aaaatgctac
    15541 acacttgctt acgagtagaa aatcttgaaa aatcaatcgc attttatcaa gatgcttttg
    15601 gttttaaaga attgcgtcgc agagattttc cagaccatgc cttcacgatt gtctatctag
    15661 gtcttgaggg tgatgactat gagttggagt tgacttataa ctacgatcac ggtccgtatg
    15721 tggttggtga tgggtttgcc catatcgccc tcagtacacc tgatcttgag gcacttcatc
    15781 aagaacacag tgcaaaaggc tatgaagtga ctgagccaaa tggtctacca ggaactacac
    15841 caaactatta ctttgtcaaa gaccctgatg gatataaggt cgaagtcatt cgtgaaaaat
    15901 aatttcgtgc aagttaagta gaatattcgt tttttacttg acttttttta gctgaaagag
    15961 tatactaata aaaattcaac ctttaagggg agtccagaga gactcacaag gtgtcagata
    16021 aaagaatagt gcaattttct agaggagact ttttgagtgt gctctcttgt gttgtacgat
    16081 tttaactgag gccttgcact agcaaggtct tttctttatc tggtcccctt aaaatttaag
    16141 gaggaaaagt tatgaatccc acatgtaaga agcgtttggg tgtcattcgg ttggaaacca
    16201 tgaaggtggt tgcacaagag gaaatcgcgc cagcaatctt tgaattagtc ctagaaggag
    16261 aaatggttga agccatgcga gcaggccaat ttcttcatct gcgtgtacca gacgatgccc
    16321 atcttttacg tcgtcctatt tcaatttcgt ctattgacaa ggcaaacaag cagtgtcacc
    16381 tcatttatcg gattgaggga gctgggactg caattttttc aaccttaagt cagggagaca
    16441 ctcttgatgt gatggggcct cagggaaatg gttttgactt gtctgacctt gatgagcaga
    16501 atcaggttct ccttgttggt ggtgggattg gtgttccacc cttgcttgag gtggccaagg
    16561 aattgcatga acgtggagtg aaagtagtga cagtcctcgg ttttgctaat aaggatgctg
    16621 ttattttgaa aacggaattg gctcagtatg gtcaggtctt tgtaacgaca gatgatggtt
    16681 cttatggcat caagggaaat gtttccgttg ttatcaatga tttagatagt cagtttgatg
    16741 ctgtttactc gtgtggggct ccaggaatga tgaagtatat caatcaaacc tttgatgatc
    16801 acccaagagc ctatttatct ctggaatctc gtatggcttg tgggatggga gcttgctatg
    16861 cctgtgttct aaaagtacca gaaagcgaga cggtcaacca acgcgtctgt gaagatggtc
    16921 ctgttttccg cacaggaaca gttgtattat aaggagaaaa ttatgactac aaatcgatta
    16981 caagtgtctc tacctggttt ggatttgaaa aatccgatta ttccagcatc aggttgtttt
    17041 ggctttggac aagagtatgc caagtactat gatttaaacc ttttaggttc tattatgatc
    17101 aaggcgacaa cccttgaacc acgttttggg aacccaactc caagagtagc agagacgcct
    17161 gctggtatgc tcaatgcaat tggcttgcaa aatcctggtt tagaggttgt tttggctgaa
    17221 aagctacctt ggctggaaag agaatatcca aatcttccta ttattgccaa tgtagctggt
    17281 ttttcaaaac aagagtatgc agctgtttct catgggattt ccaaggcaac taatgtaaaa
    17341 gctatcgagc tcaatatttc ttgtcccaat gttgaccact gtaatcatgg acttttgatt
    17401 ggtcaagatc cagatttggc ttatgatgtg gtgaaagcag ctgtggaagc ctcagaagtg
    17461 ccagtttatg tcaaattaac cccgagtgtg accgatatcg ttactgtcgc aaaagctgca
    17521 gaagatgcgg gagcaagtgg cttgaccatg atcaatactc tggttggaat gcgctttgac
    17581 ctcaaaacca gaaaaccaat cttggccaat ggaacaggtg gaatgtcagg tccagcagtc
    17641 tttccagtag ccctcaaact catccgccaa gtagcccaaa caacagacct gcctatcatt
    17701 ggaatggggg gagtggattc ggctgaagct gccctagaaa tgtatctggc tggggcatct
    17761 gctatcggag ttggaacagc taactttacc aatccttatg cctgccctga catcatcgaa
    17821 aatttaccaa aagtcatgga taaatacggt attagcagtc tggaagaact ccgtcaggaa
    17881 gtaaaagagt ctctgaggta aactgcaatc aatctgttct tgatttttta ttagtttgta
    17941 atatgaattt aggagaattt tggtacaata aaataaataa gaacagagga agaaggttaa
    18001 tgaagaaagt aagatttatt tttttagctc tgctattttt cttagctagt ccagagggtg
    18061 caatggctag tgatggtact tggcaaggaa aacagtatct gaaagaagat ggcagtcaag
    18121 cagcgaatga gtgggttttt gatactcatt atcaatcttg gttctatata aaagcagatg
    18181 ctaactatgc tgaaaatgaa tggctaaagc aaggtgacga ctatttttac ctcaaatctg
    18241 gtggctatat ggccaaatca gaatgggtag aagacaaggg agccttttat tatcttgacc
    18301 aagatggaaa gatgaaaaga aatgcttggg taggaacttc ctatgttggt gcaacaggtg
    18361 ccaaagtaat agaagactgg gtctatgatt ctcaatacga tgcttggttt tatatcaaag
    18421 cagatggaca gcacgcagag aaagaatggc tccaaattaa agggaaggac tattatttca
    18481 aatccggtgg ttatctacta acaagtcagt ggattaatca agcttatgtg aatgctagtg
    18541 gtgccaaagt acagcaaggt tggctttttg acaaacaata ccaatcttgg ttttacataa
    18601 aagaaaatgg aaactatgct gataaagaat ggattttcga gaatggtcac tattattatc
    18661 taaaatctgg tggctacatg gcagccaatg aatggatttg ggataaggaa tcttggtttt
    18721 atctcaaatt tgatgggaaa atagctgaaa aagaatgggt ctacgattct catagtcaag
    18781 cctggtacta cttcaaatcc ggtggttaca tgacagccaa tgaatggatt tgggataagg
    18841 aatcttggtt ttacctcaag tctgatggca aaatggctga aaaagaatgg gtctacgatt
    18901 ctcatagtca agcctggtac tacttcaaat ctggtggtta catggcgaaa aatgagacag
    18961 tagatggtta tcagcttgga agcgatggta aatggcttgg aggaaaagct acaaatgaaa
    19021 atgctgctta ctatcaagta gtgcctgtta cagccaatgt ttatgattca gatggtgaaa
    19081 agctttccta tatatcgcaa ggtagtgtcg tatggctaga taaggataga aaaagtgatg
    19141 acaagcgctt ggctattact atttctggtt tgtcaggcta tatgaaaaca gaagatttac
    19201 aagcgctaga tgctagtaag gactttatcc cttattatga gagtgatggc caccgttttt
    19261 atcactatgt ggctcagaat gctagtatcc cagtagcttc tcatctttct gatatggaag
    19321 taggcaagaa atattattcg gcagatggcc tgcattttga tggttttaag cttgagaatc
    19381 ccttcctttt caaagattta acagaggcta caaactacag tgctgaagaa ttggataagg
    19441 tatttagttt gctaaacatt aacaatagcc ttttggagaa caagggcgct acttttaagg
    19501 aagccgaaga acattaccat atcaatgctc tttatctcct tgcccatagt gccctagaaa
    19561 gtaactgggg aagaagtaaa attgccaaag ataagaataa tttctttggc attacagcct
    19621 atgatacgac cccttacctt tctgctaaga catttgatga tgtggataag ggaattttag
    19681 gtgcaaccaa gtggattaag gaaaattata tcgatagggg aagaactttc cttggaaaca
    19741 aggcttctgg tatgaatgtg gaatatgctt cagaccctta ttggggcgaa aaaattgcta
    19801 gtgtgatgat gaaaatcaat gagaagctag gtggcaaaga ttagtactat aagtgaatat
    19861 gatttgagtg aatagtaagt taaaaatcct gatttcaagt aaaatcagga ttttttcatg
    19921 gatgcaattt ttttggagtc tggtgtgacg cggagggtct tttgtcctgt gtaagtgaca
    19981 aagccgggtt ttccaccagt tggtttattg agttttttga cttcaatcat atctacctgc
    20041 accagattcg acaggcgccc ttgagagaag taggcagcta actctgctgc gtctgtcttg
    20101 actgcatcag atgggtcaag atttcctgag atgacaacat ggcttccagg aatgtcctta
    20161 gcatggaacc aaagttcctc cttgcgggcc attttaaagg tcaattcctc attttgaaga
    20221 ttgtttcgtc cgacatagat gatggttttg ccatcgcttg ctagatattg ttctagtttt
    20281 ttgcgtttct ggattttctc ccgttgtctt ctgcggataa aacctgtttg aatcaattct
    20341 tcacggattt cagcgatttc ttccagtcca gcttggttga ggacggtttc tacactttcc
    20401 agatagagaa tagtggcttt ggtttcttca atcaaatcag tcaagtattt gacagcttct
    20461 ttgagtttct gataccgttt aaaatagcgt tgggcattct ggttgggagt cagagcctta
    20521 tcaagcgcaa tcatgatagg ttggttggta tagtagttgt ctaggataac ctggtcttgg
    20581 tcgttaggca cttggtggag gaaggttgtc agcaattctc ctttttgacg aaattcttca
    20641 gcgttgtctg tcgccagtaa ctctttttcc tgttttttga gtttgtgtcg gtttttctga
    20701 agttcatttt caacacgacg aatcagttca ctggcctgct gtttgacgcg gtcgcgctca
    20761 gccttatcct tatagtaggt gtccaacaaa tcagaaagat ttgcaagagg ctctcccacc
    20821 tgatttgcaa aaggaactgg actgaaggaa gtctcagtca agcatggctt ggtttcttga
    20881 ttgaaaaaat ttcggaaagc ggaaagtttt tcactaacca gtatcctttc caattcattt
    20941 gccgtatcgc gtcccagacc ttgaaagagg ctttgaagat tttttgctgt tagttcttgg
    21001 gtttgcagga tttcaaagag cttttcatcc ttgatagtaa aaggattgag agattttgta
    21061 cttggcggag cgatataggt cgatcctgga agtaaggtgc ggtagctatt ttgtgaaaag
    21121 ccgacgtgtt tgataacttc gaggatttta tgactgcttt tatcgaccag tagaatatta
    21181 ctgtgtttcc ccataatttc gataatcaag gtagcctgga tatggtctcc aatctcgttt
    21241 ttattggaaa ctgtaatttc cacaatacgg tcattttcca cttgctcaat cgactcaatc
    21301 agggccccct gcaaatactt tctcaaaacc atgataaagg tagaaggttg ggctggattt
    21361 tcaaaagtcg tttgggtcag ctgaatgcgt ccaaaaactg gatgggcaga aaggagcagg
    21421 cgatggcttt ggcgattgct gcggatttgc aagaccaact cttgttcaaa aggctgattg
    21481 attttctgga tgcgaccatt cactaattcg cttcgcaatt cctcaactat gtggtgtaaa
    21541 aaaaatccgt caaatgacat cgttctctcc ttgtgattgt attccatagt attatatcaa
    21601 aaaggtagaa taaaatcatg gaaatgtggt ataataaagc caagtaaaga gaaacgagaa
    21661 gcacatgtat attgaaatgg tagatgaaac tggtcaagtt tcaaaagaaa tgttgcaaca
    21721 aacccaagaa attttggaat ttgcagccca aaaattagga aaagaagaca aggagatggc
    21781 agtcactttt gtgaccaatg agcgtagtca tgaacttaat ctggggtacc gtgacaccga
    21841 ccgtccgaca gatgtcatca gccttgagta taaaccagaa ttggaaattg cctttgacga
    21901 agaggatttg cttgaaaatc cagaattggc agagatgatg tctgagtttg atgcctatat
    21961 tggggaattg ttcatctcta tcgataaggc tcatgagcag gccgaagaat atggtcacag
    22021 ctttgagcgt gagatgggct tcttggcagt acacggcttt ttacatatta acggctatga
    22081 tcactatact ccggaagaag aagcggagat gttcggttta caagaagaaa ttttgacagc
    22141 ctatggactc acaagacaat aaacgaaaat ggaaaaatcg tgacctgata tccagtttag
    22201 aatttgccat cacagggatt tttactgcta tcaaggaaga acgcaatatg cgaaaacatg
    22261 cagtgacggc tctagtggtc atccttgcag gttttggttt tcaggtgtca cgaatcgaat
    22321 ggctctttct cctattgagc attttcttgg tggtagcctt tgaaattatc aattctgcta
    22381 tcgaaaatgt ggtggatttg gccagtcact atcacttttc catgctggct aaaaatgcca
    22441 aggatatggc ggctggcgcg gtattagtgg tttctctttt tgcagcctta acaggcgcat
    22501 tgatttttct cccacgaatc tgggatttat tattttaaac agtaagagga aattatgact
    22561 tttaaatcag gctttgtagc cattttagga cgtcccaatg ttgggaagtc aaccttttta
    22621 aatcacgtta tggggcaaaa gattgccatc atgagtgaca aggcgcagac aacgcgcaat
    22681 aaaatcatgg gaatttacac gactgataag gagcaaattg tctttatcga cacaccaggg
    22741 attcacaaac ctaaaacagc tctcggagat ttcatggttg agtctgccta cagtaccctt
    22801 cgcgaagtgg acactgttct tttcatggtg cctgctgatg aagcgcgtgg taagggggac
    22861 gatatgatta tcgagcgtct caaggctgcc aaggttcctg tgattttggt ggtgaataaa
    22921 atcgataagg tccatccaga ccagctcttg tctcagattg atgacttccg taatcaaatg
    22981 gactttaagg aaattgttcc aatctcagcc cttcagggaa ataacgtgtc tcgtctagtg
    23041 gatattttga gtgaaaatct ggatgaaggt ttccaatatt tcccgtctga tcaaatcaca
    23101 gaccatccag accgtttctt agtttcagaa atggttcgcg agaaagtctt gcacctaact
    23161 cgtgaagaga ttccgcattc tgtagcagta gttgttgact ctatgaaacg agacgaagag
    23221 acagacaagg ttcacatccg tgcaaccatc atggtcgagc gcgatagcca aaaagggatt
    23281 atcatcggta aaggtggcgc tatgcttaag aaaatcggta gcatggcccg tcgtgatatc
    23341 gaactcatgc taggagacaa ggtcttccta gaaacctggg tcaaggtcaa gaaaaactgg
    23401 cgcgataaaa agctagattt ggctgacttt ggctataatg aaagagaata ctaagtagag
    23461 gtgggctcat gcctgcttct tgtttttaca gaaggaggac ttatgcctga attacctgag
    23521 gttgaaaccg tttgtcgttg cttagaaaaa ttgattatag gaaagaagat ttcgagtata
    23581 gaaattcgct accccaagat gattaagacg gatttggaag agtttcaaag ggaattgcct
    23641 agtcagatta tcgagtcaat gggacgtcgt ggaaaatatt tgctttttta tctgacagac
    23701 aaggttttga tttcccattt gcggatggag ggcaagtatt tttactatcc agaccaagga
    23761 cctgaacgca agcatgccca tgttttcttt cattttgaag atggtggcac gcttgtttat
    23821 gaggatgttc gcaagtttgg aaccatggaa ctcttggtgc ctgacctttt agacgcctac
    23881 tttatttcta aaaaattagg tcctgaacca agcgaacaag actttgattt acaggtcttt
    23941 caatctgccc ttgccaagtc caaaaagcct atcaaatccc atctcctaga ccagaccttg
    24001 gtagctggac ttggcaatat ctatgtggat gaggttctct ggcgagctca ggttcatcca
    24061 gctagatctt cccagacttt gacagcagaa gaagcgacag ctattcatga ccaaaccatt
    24121 gctgttttga gccaggctgt tgaaaaaggc ggttccacca ttcggaccta taccaatgcc
    24181 tttggggaag atggaagcat gcaggatttt catcaggtct atgataagac tggtcaagga
    24241 tgtgtacgct gtggtaccat cattgagaaa attcaactag gtggacgtgg aacccacttt
    24301 tgtccaaact gtcaaaggag ggactgatgg gaaaaatcat cggaatcact gggggaattg
    24361 cctctggtaa gtcaactgtg acaaattttc taagacagca aggctttcaa gtagtggatg
    24421 ccgacgcagt cgtccaccaa ctacagaaac ctggtggtcg tctgtttgag gctctagtac
    24481 agcacttttg gcaagaaatc attcttgaaa acggagaact caatcgccct ctcctagcta
    24541 gtctcatctt ttcaaatcct gatgaacgag aatggtctaa gcaaattcaa ggggagatta
    24601 tccgtgagga actggctact ttgagagaac agttggctca gacagaagag attttcttca
    24661 tggatattcc cctacttttt gagcaggact acagcgattg gtttgctgag acttggttgg
    24721 tctatgtgga ccgagatgcc caagtagaac gcttaatgaa aagggaccag ttgtccaaag
    24781 atgaagctga gtctcgtctg gcagcccagt ggcctttaga aaaaaagaaa gatttggcca
    24841 gccaggttct tgataataat ggcaatcaga accagcttct taatcaagtg catatccttc
    24901 ttgagggagg taggcaagat gacagagatt aactggaagg ataatctgcg cattgcctgg
    24961 tttggtaatt ttctgacagg agccagtatt tctttggttg taccttttat gcctatcttc
    25021 gtggaaaatc taggtgtagg gagtgagcaa gtcgcttttt atgcaggctt agcaatttct
    25081 gtctctgcta tttccgcggc gctcttttct cctatttggg gtattcttgc tgacaaatac
    25141 ggccgaaaac ccatgatgat tcgggcaggt cttgctatga ctatcactat gggaggcttg
    25201 gcctttgtcc caaatatcta ttggttaatc tttcttcgtt tactaaacgg tgtatttgca
    25261 ggttttgttc ctaatgcaac ggcactgata gccagtcagg ttccaaagga gaaatcaggc
    25321 tctgccttag gtactttgtc tacaggcgta gttgcaggta ctctaactgg tccctttatt
    25381 ggtggcttta tcgcagaatt atttggcatt cgtacagttt tcttactggt tggtagtttt
    25441 ctatttttag ctgctatttt gactatttgc tttatcaagg aagattttca accagtagcc
    25501 aaggaaaagg ctattccaac aaaggaatta tttacctcgg ttaaatatcc ctatcttttg
    25561 ctcaatctct ttttaaccag ttttgtcatc caattttcag ctcaatcgat tggccctatt
    25621 ttggctcttt atgtacgcga tttagggcag acagagaatc ttctttttgt ctctggtttg
    25681 attgtgtcca gtatgggctt ttccagcatg atgagtgcag gagtcatggg caagctaggt
    25741 gacaaggtgg gcaatcatcg tctcttggtt gtcgcccagt tttattcagt catcatctat
    25801 ctcctctgtg ccaatgcctc tagccccctt caactaggac tctatcgttt cctctttgga
    25861 ttgggaaccg gtgccttgat tcccggggtt aatgccctac tcagcaaaat gactcccaaa
    25921 gccggcattt cgagggtctt tgccttcaat caggtattct tttatctggg aggtgttgtt
    25981 ggtcccatgg caggttctgc agtagcaggt caatttggct accatgctgt cttttatgcg
    26041 acgagccttt gtgttgcctt tagttgtctc tttaacctga ttcaatttcg aacattatta
    26101 aaagtaaagg aaatctagtg cgagtaaaaa ttaatctcaa atgctcctct tgtggcagta
    26161 tcaattacct aaccagtaaa aattcaaaaa cccatccaga caagattgag gttttaaagt
    26221 attgtcccaa ggaaagaaaa gtaaccctac atcttgaatc taagtagatt ttatggtaaa
    26281 ataataggga ttttaaggag tttgatatgt ataacctatt attaaccatt ttattagtat
    26341 tatctgttgt gattgtgatt gcaattttca tgcaaccaac caaaaaccaa tccagcaatg
    26401 tatttgatgc cagttcaggt gatttgtttg aacgtagtaa agctcgcggt tttgaagctg
    26461 taatgcagcg tttgacaggg attttagtct ttttctggct agccattgcc ttagcattga
    26521 cggtattatc aagtagataa gaaaataatg ggcaggacta ggtctttgcc tctttttatt
    26581 tttaaaggat gtttgagaag gttttacagt aaaagaaaat taaaaaatct agaaagaaaa
    26641 tatgaaagat agaataaaag aatatttaca agacaaggga aaggtgactg ttaatgattt
    26701 ggctcaggct ttgggaaaag acagttccaa ggattttcgt gagttgatta aaaccttgtc
    26761 cttaatggaa agaaagcacc aaattcgttt tgaagaagat ggtagtctga cattagaaat
    26821 taagaaaaaa catgagatta ccctcaaggg gatttttcat gcccataaaa atggctttgg
    26881 ctttgttagt ctggaaggcg aggaggacga cctttttgta gggaaaaatg atgtcaacta
    26941 tgctattgat ggtgataccg tcgaggtagt gattaagaaa gtcgctgacc gcaataaggg
    27001 aacagcagca gaagccaaaa ttattgatat cctagaacac agtttgacaa cagttgtcgg
    27061 gcaaatcgtt ctggatcagg aaaaacctaa gtatgctggc tatattcgtt caaaaaatca
    27121 gaaaatcagt caaccgattt atgttaagaa accagcccta aaattagaag gaacagaagt
    27181 tctcaaagtc tttatcgata aatacccaag caagaaacat gatttctttg tcgcgagtgt
    27241 tctcgatgta gtgggacact caacggatgt cggaattgat gttcttgagg tcttggaatc
    27301 aatggacatt gtatccgagt ttccagaagc tgttgttaag gaagcagaaa gtgtgcctga
    27361 tgctccgtct caaaaggata tggaaggtcg tctggatcta agagatgaaa ttacctttac
    27421 cattgacggt gcggatgcca aggacttgga cgatgcagtg catatcaagg ctctgaaaaa
    27481 tggcaatctg gagcttgggg ttcacatcgc agatgtttct tattatgtga ccgaggggtc
    27541 tgcccttgac aaggaagccc ttaaccgtgc gacttctgtt tacgtgacag accgagtggt
    27601 gccaatgctt ccagaacgac tatcaaatgg catctgctct ctcaatcccc aagttgaccg
    27661 cctgacccag tctgctatta tggagattga taaacatggt cgtgtggtca actataccat
    27721 tacacaaaca gttatcaaga ccagttttcg tatgacctat agcgatgtca atgatatcct
    27781 agctggcgat gaagaaaaga gaaaagaata tcataaaatt gtatcaagta tcgaactcat
    27841 ggccaagctt catgaaactt tagaaaacat gcgtgtgaaa cgtggagctc tcaattttga
    27901 taccaatgaa gcgaagattt tagtggataa acaaggtaag cctgttgata tcgttcttcg
    27961 gcagcgtggt attgccgagc ggatgattga gtcttttatg ttgatggcta atgaaacagt
    28021 tgccgaacat ttcagcaagt tggatttgcc ttttatctat cgaattcacg aggagcctaa
    28081 ggctgaaaag gttcagaagt ttattgatta tgcttcgagt tttggcttgc gcatttatgg
    28141 aactgccagt gagattagtc aggaggcact tcaagacatc atgcgtgctg ttgagggaga
    28201 accttatgca gatgtattgt ccatgatgct tcttcgctct atgcagcagg ctcgttattc
    28261 ggagcacaat cacggccact atggactagc tgctgactat tatactcact ttaccagtcc
    28321 aattcgtcgt tatccagacc ttcttgttca ccgtatgatt cgggattacg gccgttctaa
    28381 ggaaatagca gagcattttg aacaagtgat tccagagatt gcgacccagt cttccaaccg
    28441 tgaacgtcgt gccatagaag ctgagcgtga agtcgaagcc atgaaaaagg ctgagtatat
    28501 ggaagaatac gtgggtgaag agtatgatgc agttgtatca agtattgtca aattcggtct
    28561 ctttgtcgaa ttgccaaaca cagttgaagg cttgattcac atcactaatc tacctgaatt
    28621 ttatcatttc aatgagcgtg atttgactct tcgtggagaa aaatcaggta tcactttccg
    28681 agtgggtcag cagatccgta tccgtgttga aagagcggat aaaatgactg gagagattga
    28741 tttttcattc gtacctagtg agtttgatgt gattgaaaaa ggcttgaaac agtctagtca
    28801 tagtggcaga gggcgtggtt caaatcgtcg ttcggataag aaggaagaca agagaaaatc
    28861 aggacgctca aatgataagc gtaagcattc acaaaaagac aagaagaaaa aaggaaagaa
    28921 acctttttac aaggaagtag ctaagaaagg agccaagcat ggcaaagggc gagggaaagg
    28981 tcgtcgcaca aaataaaaag gcacgccacg actatacaat cgtagatacg ctagaggcag
    29041 ggatggtcct gactggaact gaaatcaaga gtgtacgagc tgctcgaatt aatctcaagg
    29101 atggctttgc tcaagtgaaa aatggagaag tttggctgag caatgttcat atcgcgcctt
    29161 acgaagaggg caatatctgg aaccaggaac cagaacgtcg tcgtaaactc ctgctccata
    29221 aaaagcaaat tcaaaaattg gaacaagaga ccaaagggac aggaatgacc ttagttcccc
    29281 ttaaggtcta tataaaagat ggctacgcta agcttctttt aggacttgcc aaagggaagc
    29341 atgactatga caaacgggag tctatcaaac gtcgtgagca aaatcgagat atcgcgcgtg
    29401 tgatgaaagc tgttaatcag cgataaaaag aggaattgaa aatggaaaaa ttagttgcct
    29461 ataaacgcat gcctttgtgg aataaacaaa caatgcctga agatgttcag caaaagcaca
    29521 atacaaaagt tgggacttgg gggaaaatta ctgtcttgaa gggagctctc aagtttattg
    29581 aattgacaga agaaggggaa gttctagctg aacacctctt tgaagcaggg gcagacaatc
    29641 caatggccca acctcaagcc tggcaccgag tggaagctgc cacagatgat gtggaatggt
    29701 acttggaatt ttattgtaaa cctgaggatt attttgctaa aaaatacaat accaatcctg
    29761 ttcattcaga ggtcctagag gccatgcaga cagtgaaaca agggaaagct ttggatttgg
    29821 gttgtggtca ggggcgtaat tctctttttc tagcccagca agattttgat gtgacggctg
    29881 tagatcaaaa tggactagct cttgaaatct tgcaaagcat tgtggagcag gaagatttgg
    29941 acatgcctgt tggcctttac gatatcaatt cagctagcat tgaacaagaa tatgatttta
    30001 tcgtttcaac agttgttctc atgtttctac aagcggaccg cattccagct attattcaaa
    30061 atatgcagga gaaaaccagt gttggtggtt acaaccttat cgtttgtgcc atggacacgg
    30121 aggattatcc ttgctcggtt aatttcccat tcacctttaa agaaggagaa ctggcagact
    30181 attacaagga ttgggaattg gttaagtaca atgaaaatcc aggccatttg caccgtcgcg
    30241 atgagaatgg caatcgtatt caactacgct ttgcgacctt actagctaag aaaatcaagt
    30301 aaacacacat gaagattagg aattttcctg atcttttttc ttttttacga atgatataga
    30361 aaaggaggga attcatgttt gttgcgagag atgctagggg agaattggta aatgtgttag
    30421 aggataaact tgagaagcaa gcatacacct gcccagcttg tggaggccag ctccatttgc
    30481 gtcaaggacc aagtgtacgg acgcattttg cccataaatc cttaaaagac tgtgattttt
    30541 tctttgaaaa tgaaagtcca gaacacctgg ccaataagga atccctctat cactggttga
    30601 aaaaagagac aaaggttcaa ttagagtacc cgctttcaga acttaaacag attgcggatg
    30661 tatttgtaaa tggcaatcta gctctagaag ttcagtgtag tcccttgcct cagaaagtcc
    30721 ttaaagagcg aagtgaggga tatcgtagtc agggttacca agtactgtgg ttgctgggtc
    30781 aaaaactgtg gctcaaggag cgtttgactc gtctacagca aggttttctt tatttcagtc
    30841 aaaacatggg cttttatgtt tgggaattag acaaggaaaa acaagtttta agactcaaat
    30901 acctgattta ccaggatctc cgcggtaaac tccattatca aatcaaggaa ttttcctatg
    30961 gtcaaggta
//
LOCUS       DAVBBS010000021        30074 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVBBS010000021 DAVBBS010000000
VERSION     DAVBBS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30074
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.021"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            51..1100
                     /locus_tag="VQU32_000933"
     CDS             51..1100
                     /locus_tag="VQU32_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET2472404.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAVHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGSNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(1200..5042)
                     /gene="spuA"
                     /locus_tag="VQU32_000934"
     CDS             complement(1200..5042)
                     /gene="spuA"
                     /locus_tag="VQU32_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HET2472405.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPGNANASLEKTEEKPAASPA
                     DPAPQTGQDRSSEPTTSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDD
                     VEKPSENWPNGALSFKDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVE
                     KLVPKMNEAWLDQDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQW
                     PDGTDFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQ
                     IFLKDDDESIYTNPYYVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHL
                     GNKVTITDVAIDEAGKKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGK
                     LGADLKEEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDST
                     NKLGITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSDDSKIDDAHKVAKAAFVDP
                     AKLGPQDLTYGKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDY
                     LKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSD
                     PKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDLFEDLEPNYYHFMDADGTPR
                     TSFGGGRLGTTHHMTKRLLIDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARAL
                     NPNLIMLGEGWRTYAGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPA
                     FITGGKRDVNTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKA
                     ENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHL
                     LRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQS
                     TDAFRLKSLQDIKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKARE
                     FNLGTAFAHLRNAEVLADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTS
                     HESTAEEKPDSTPSKPEHQNEASHPEHQDPAPEARPDSTKPDTKVADAENKPSQATAD
                     SQAEQPAQEAQASSVKEAVRNESVENSSKKNIPATPDRQAELPNTGIKNENKLLFAGI
                     SLLALLGLGFLLKNKKEN"
     gene            5459..5872
                     /gene="rpsL"
                     /locus_tag="VQU32_000935"
     CDS             5459..5872
                     /gene="rpsL"
                     /locus_tag="VQU32_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET2472406.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            5892..6362
                     /gene="rpsG"
                     /locus_tag="VQU32_000936"
     CDS             5892..6362
                     /gene="rpsG"
                     /locus_tag="VQU32_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET2472407.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            6787..8868
                     /gene="fusA"
                     /locus_tag="VQU32_000937"
     CDS             6787..8868
                     /gene="fusA"
                     /locus_tag="VQU32_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET2472408.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            8972..13363
                     /locus_tag="VQU32_000938"
     CDS             8972..13363
                     /locus_tag="VQU32_000938"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HET2472409.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPAFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFESVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            13465..13728
                     /locus_tag="VQU32_000939"
     CDS             13465..13728
                     /locus_tag="VQU32_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HET2472410.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            13721..13999
                     /locus_tag="VQU32_000940"
     CDS             13721..13999
                     /locus_tag="VQU32_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HET2472411.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            14020..14172
                     /locus_tag="VQU32_000941"
     CDS             14020..14172
                     /locus_tag="VQU32_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472412.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            14194..15435
                     /locus_tag="VQU32_000942"
     CDS             14194..15435
                     /locus_tag="VQU32_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET2472413.1"
                     /translation="MVLPNFKENLGKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFKNAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            15445..15675
                     /locus_tag="VQU32_000943"
     CDS             15445..15675
                     /locus_tag="VQU32_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET2472414.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            15898..16620
                     /locus_tag="VQU32_000944"
     CDS             15898..16620
                     /locus_tag="VQU32_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET2472415.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHVLLSPKRHVDKTYQAQVKGIMTQEDVETFIKGIPLKDFTC
                     QPARLEILSTDAEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            16837..18171
                     /gene="pepC"
                     /locus_tag="VQU32_000945"
     CDS             16837..18171
                     /gene="pepC"
                     /locus_tag="VQU32_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HET2472416.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALECRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(18227..19138)
                     /locus_tag="VQU32_000946"
     CDS             complement(18227..19138)
                     /locus_tag="VQU32_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2472417.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(19162..19965)
                     /locus_tag="VQU32_000947"
     CDS             complement(19162..19965)
                     /locus_tag="VQU32_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HET2472418.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(19993..20982)
                     /gene="manX"
                     /locus_tag="VQU32_000948"
     CDS             complement(19993..20982)
                     /gene="manX"
                     /locus_tag="VQU32_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HET2472419.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            complement(21215..22234)
                     /gene="adhP"
                     /locus_tag="VQU32_000949"
     CDS             complement(21215..22234)
                     /gene="adhP"
                     /locus_tag="VQU32_000949"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HET2472420.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLTLAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(22573..23385)
                     /locus_tag="VQU32_000950"
     CDS             complement(22573..23385)
                     /locus_tag="VQU32_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2472421.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKRTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            23575..24993
                     /locus_tag="VQU32_000951"
     CDS             23575..24993
                     /locus_tag="VQU32_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HET2472422.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            25046..25753
                     /locus_tag="VQU32_000952"
     CDS             25046..25753
                     /locus_tag="VQU32_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2472423.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVTGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            25848..26822
                     /gene="folP"
                     /locus_tag="VQU32_000953"
     CDS             25848..26822
                     /gene="folP"
                     /locus_tag="VQU32_000953"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET2472424.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGANLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEDLMVAFFERALARAAEAGITPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            26824..28146
                     /locus_tag="VQU32_000954"
     CDS             26824..28146
                     /locus_tag="VQU32_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET2472425.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGIPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            28127..28681
                     /gene="folE"
                     /locus_tag="VQU32_000955"
     CDS             28127..28681
                     /gene="folE"
                     /locus_tag="VQU32_000955"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET2472426.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            28724..29536
                     /gene="folK"
                     /locus_tag="VQU32_000956"
     CDS             28724..29536
                     /gene="folK"
                     /locus_tag="VQU32_000956"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET2472427.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFEFYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIYILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPTLKQPIRNLYDALKK"
     gene            complement(29581..29898)
                     /locus_tag="VQU32_000957"
     CDS             complement(29581..29898)
                     /locus_tag="VQU32_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HET2472428.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMYGRETILLPEEN
                     DLF"
BASE COUNT         8527 a         5841 c         7006 g         8700 t
ORIGIN      
        1 ttagaataat ttttcaaatt gaaggacaga gatagacaag gagaatcaca atggtagaat
       61 tgggaatttc aacatttggg gaaataacgg agcttgaagg gactggacaa acttacagtc
      121 atgctgaacg cattcgccag ttggtggcag agattgagct ggctgacaag gttggtttgg
      181 acgtatatgg gattggcgag caccatcggg cggattttgc agtatcagcc ccagagattg
      241 tcctggccgc tggggcagtc aataccaaga aaatccgttt gaccagtgca gtcagcattc
      301 tgtcaagcat ggatccgatt cgtttgttcc aacagtatgc cactatcgat gctttgtcaa
      361 atggacgagc ggagattatg gctggaaggg gctctttcac ggaatctttc cctctgtttg
      421 gttatgactt gaaagactac gactctcttt ttgatgagaa attagacttg cttcagttag
      481 tcaatgaaaa gaccaagtta gactggcaag gtcgattgac tcaaacgatt gctggtaaag
      541 aagtttatcc tcgtccagtt caggacaaat tgcccttgtg gatagctaca ggtggtcatg
      601 tcgaatcaac agtgaagatt gctcaggctg gtctaccgat tgtatatgct attattggtg
      661 gtaatccacg ttattttaaa aagttgattc aggcttatcg tgagattgga agcgaagcgg
      721 tccatgctga caaagacttg aaagtgggag cccattcttg ggggtggatt gcggaagatg
      781 gtgagcaggc ggtgaaagat tatttccatc cgactaagca agtggtggat gctatttcca
      841 aagaccgtcc gcactggcag gaattgcgtt atgagcaata tttggagcag gtagggtcaa
      901 atggcgccat gtttgttggc aatccagatc aggtggccga aaaattgatt cgcatgatag
      961 aggatttagg attggaccgc ttcatgcttc atctaccgct tggttctatg cctcatgacc
     1021 aggttctgag agctattgaa ctcttcggaa cacaagttgc acccaaagta cgagcttatt
     1081 ttgctatgaa agaggcttaa taaaaaatcc taatcaagtt tattaggaca acaaatattg
     1141 tacgaaaatt aatcccccga gctataatgc ttgggggatt tttctatagg agggctagtt
     1201 tagttctctt ttttattttt tagtaagaaa ccgagaccaa ggagcgcaag gaggctgatt
     1261 cctgcaaata ggagtttgtt ttcgttttta attcctgtat ttggaagttc agcttgtcta
     1321 tctggggttg caggtatatt tttcttgcta gagttttcta ccgattcgtt tcgaaccgct
     1381 tcttttacag atgatgcttg tgcttcttgt gctggttgtt cagcttgtga atcagctgta
     1441 gcttggctag gtttattttc cgcatcagct actttggtat ctggtttagt agaatcaggt
     1501 ctagcttctg gagctggatc ttgatgttca gggtgagaag cttcattttg atgttcaggc
     1561 ttggaagggg ttgagtctgg tttctcttct gcagttgact catggctagt tccattttga
     1621 gagactcgaa gaacagtagc tgtaagggca ttcaatttca agcctttttc agtccattca
     1681 agtcctttcg ggttggcaat tccgactgat cctgcttggt tttcatctgc caaaacttcc
     1741 gcatttctta gatgtgcaaa ggcagttccc aaattaaatt cgcgagcttt ttcatccgca
     1801 ttgacaaaga ctgcgtagat atcgccgttt ggagcagtga tttgatagcc aatcactaca
     1861 tcctcttttt ccacaccatt ttggcctggg acagtgatga ggtggacacg gtctttgata
     1921 tcttgaagac tcttaagtcg gaaggcatct gtagattgac gaagggcaat caaacctttc
     1981 atatagtcac ggctcttgac attttcagga taagctttac catctgtagc cttagtccag
     2041 tcaaacttgt tgactgcatc actagaatcg taagagtcat ggatgaagta aggatagtca
     2101 aatgggttgc cgtccttatc acgcaacaag tgagatttgt ttggaacctt atcctctgct
     2161 actggagtct tgtaggctgg gtcacggaat tgtttagtac gtccatattc ctgaccggag
     2221 tggataaatg gagttccttg agctgtcaag accatgagat ttccaagtcg taaacgacgg
     2281 tggatttcag catagttctc agccttgctt gggtcttttt tgatagactg ggcaatgatg
     2341 tcaaagaggg tcaagttatc atgggctgcg atgtattgga tgacatctcc agggctgtca
     2401 gcttcaaagt tagttggttg agcaatgaga tttttaaaga tggtgttgac atcacgcttg
     2461 ccacctgtga taaaggcagg ttgaccttcg tttggataac cagatttgag gttgttacgg
     2521 atgtcatctg aaaagacagc gacagtatcg gtatgtttca tccaatcttg gtcagcagct
     2581 ttagtaggca tgttttcatc accggcatag gttctccaac cttcaccaag catgatgagg
     2641 tttggattga gggcgcgtgc agccttgtaa gcttcttcga tagaagcggc gtcatggtct
     2701 cccatcatat cgaaacggaa gccatccact ttgtaggtat caactaggta tttgatagag
     2761 tcaattagga gccgtttggt catatggtgg gttgtcccca agcgtccacc accaaagcta
     2821 gttcgaggtg tgccatcggc atccataaag tggtagtagt ttggttccaa atcttcaaag
     2881 agatcgactt tggctgtgtg gttataaacg acatctagga tagctcccat accacgtttg
     2941 tggatttcgt tgatgaggtt tttaaattct gcgattcgtt tttctggatt cttaggatcg
     3001 cttgagtaca taccagtcaa ggagaagtag ttttgagggt catatcccca gttgtagttg
     3061 ctgttgcttg aagcgtagtc agacaagcgt tcatggtttt tcaattcatt gacaaagtag
     3121 taagacaaga ctggaaggag ctggatatgg gttacaccca agtctttgag atagtctagt
     3181 ttttcaatga aggcttcaaa agtcccaaat ggtttggtca agtcttttgc aatggcagga
     3241 tctgaagtga aatcacgcac atgagcttcg tagataacgg cgtcttcacg agtcttgaaa
     3301 ttgtgaatct taccataagt caagtcttga ggtccgagtt tagctggatc tacaaaggcg
     3361 gctttagcca ctttatgggc atcgtcaatc ttggaatcgt cgctattcca agcagcaaga
     3421 gatttagcgt aaggatcgag tgcaagaaca gttttacctt gacgctcgat ttggtattga
     3481 taatagtagc cagtgaaatc tgtgattccg agtttgtttg tgctgtctag agtttgtttc
     3541 caagttcctc tttccccttt ttcaagagcg acagttccaa ctactttgtc agggtcattc
     3601 ttgtcgtaga caacaacaga aaccttatca gcacttggtg accaaagggt caaatcaact
     3661 tgttttcctt cttcttttag gtcagctccc agtttgccat catagctgta tgtctcatct
     3721 ttcaggcgcc agcttgtttt ggtagtgaat tggtcggaat tgtagctaac agtataagga
     3781 tgttttgtgt cagagaaatc tccgctgtag gtcactttct taccagcttc atcgattgca
     3841 acatcggtaa tagttacctt gtttcctagg tgattagtga tgttggagtg tttgaggata
     3901 tcttcttttt tagcaccgac aagtgttgaa aagctacttt caatgctaga agtgcctacg
     3961 tgttgggctc ctgtcatacg gatatcatgg acatagtatg gatttgtgta aatcgattca
     4021 tcatcgtctt ttaggaaaat ttggctatga tttttcaaat ctgtgaactt ataattttct
     4081 ttacggattt tcacgtcgtc tccttgtttg ctctcatcta gtaataaaaa tccaaattct
     4141 cttgcggctt cattaagagg aatgtcgata tagcggccat atttgcctgt agccgtaaag
     4201 tctgttccgt caggccattg agcgctactt ggatttttca catctcccca gtaccagaga
     4261 gatttcttgt catagttgcc atctgtgcgg tagtagttga cgcgaacagt tcctgcaggc
     4321 tgtggctcgt aagagaaaac cttgtaatct tggtctaacc aagcttcgtt catttttgga
     4381 actagttttt ctacagattt atcgccggtt agattttttc cagctgtatt gttgatgagg
     4441 aagctaattt tcttggcttg ttctcccttt aatttgacat ctaggtaata gccgtagtca
     4501 tctttcttgg catccttgaa ggacaaagct ccgtttggcc agttttcaga tggtttttca
     4561 acatcgtccc aagtccatag tccttgagca tccttgtttt cttcaggaag ttttttgaca
     4621 tggatacgga agtagttatc ttcgatgggc tcttctgcct tagtttcagt tgttactgga
     4681 ctagtagaag tagttggctc acttgaacga tcttgtccag tttgtggtgc tggatcggct
     4741 gggcttgcag caggtttttc ttctgttttc tctagggaag cgtttgcatt tccaggtgaa
     4801 gaaatatcac tttcgttctt accagagcca gctgctgtat cagtaagagg ttgggcaagg
     4861 gtggttgtat ttctgttgtt gggttcgtta gtggttggtg tactttcgtc ggatgagata
     4921 gttggtgtag ccatggcaag caagacaagg cttgcgccga taaggacaga accagttcca
     4981 tttttgaggg aacggatgct gtagaccatt tttttctcag tgtgagatgg tgtttttctc
     5041 ataatgattc tccaatcaat aaatttctat atcagtatag catgtagtaa aacaatatgc
     5101 aagcgttttc tttgatttgt gaaagaaatt cttacttttg agaagttttc cgtactattt
     5161 tatatatcag tataaatgac ggagataaaa atgtcacgta agccaagccg tgttattagg
     5221 aagatgatgt gttctcagct atttttattc agaaattgat ctctaacatt accaataaaa
     5281 aagaaaaata tcttgacaag caagggaaaa ttttgttaca ataatagacg gtacttttta
     5341 cttttggtct ctcaagagtg tacagggacg tgctgacaaa tgttgcaaaa gtacacacag
     5401 atgatatctg tcaccaagtg tatcatcacc aaaaataaaa aaacacagga gaatgtagat
     5461 gcctacaatt aaccaattgg ttcgcaaacc gcgtaaatca aaagtagaaa aatctaaatc
     5521 accagctttg aacgttggtt acaatagtca taaaaaagtt caaacaaacg tttcttcacc
     5581 acaaaaacgt ggtgttgcaa ctcgtgttgg aacaatgaca cctaaaaaac ctaactcagc
     5641 ccttcgtaaa ttcgctcgtg tacgtttgag caaccttatc gaagttactg cctacatccc
     5701 aggtatcgga cacaacttgc aagagcacag cgtggtgctt cttcgcggtg gacgtgtaaa
     5761 agaccttcca ggggtacgtt accatatcgt ccgtggtgca cttgatactg caggtgttaa
     5821 cgatcgtaaa caaggccgtt ctaaatacgg tactaaacgt ccaaaagcat aaggaaaggg
     5881 gataaagaga aatgagtcgt aaaaatagag ctccaaaacg tgacgtattg ccagatccgc
     5941 tatacaattc acaactagtt actcgtctta tcaaccgcgt tatgcttgat ggtaaacgtg
     6001 gtactgctgc ttcaatcgtt tacggtgctt ttgagcaaat caaagaagct actggcaacg
     6061 atgcacttga agtatttgaa acagctatgg aaaacatcat gcctgtactt gaagtacgtg
     6121 cacgtcgtgt tggtggttct aactaccaag tcccagttga agttcgtcca gaacgtcgta
     6181 caacacttgg acttcgttgg ttggtaacaa tcgctcgtct tcgtggtgaa cacacaatgc
     6241 aagaccgtct tgcaaaagaa atcttggatg ctgctaacaa cactggtgca gcagttaaga
     6301 aacgtgaaga tactcaccgt atggctgaag ctaaccgtgc attcgcacac ttccgttggt
     6361 aagataggat gcgaaagcgt taagaaagtc ccagagaaaa tagggaatcg aagcaggttg
     6421 cggttgcaac caatgagatt catctttttc tccagacttt tagcttgagc tcaactaaat
     6481 catgatgcta ggaacggtaa ggatgcaagg taaaaatagg aaactgacgc agtattcgac
     6541 gaatacaagg agttttatct ttttcacgca gcatcccgtt ccagctcaca tcggctaact
     6601 aactttagcc cgggttcaaa ttagctaaat cgattagtat tagctataac tcagcttacc
     6661 atctcgtaag ttgaaaccaa caatagcatg aaaacattga gaacgggtag gtcctgccta
     6721 tccgttttta ttaaaatcgt gttataatag aatagaaatc aaaaataaat aggagaaaca
     6781 aacctcatgg cacgcgaatt ttcacttgaa aaaactcgta atatcggtat catggctcac
     6841 gtcgatgccg gtaaaacaac aactactgag cgtattcttt actacactgg taaaatccac
     6901 aaaatcggtg aaactcacga aggtgcgtca caaatggact ggatggagca agagcaagaa
     6961 cgtggtatca cgatcacatc tgctgcgacg acagctcaat ggaacaacca ccgcgtaaac
     7021 atcatcgaca caccaggaca cgtggacttc acaatcgaag tacaacgttc tcttcgtgta
     7081 ttggatggtg cggttaccgt tcttgactca caatcaggtg ttgagcctca aactgaaaca
     7141 gtttggcgtc aagcaactga gtacggagtt ccacgtatcg tatttgccaa caaaatggac
     7201 aaaatcggtg ctgacttcct ttactctgta agcacacttc acgatcgtct tcaagcaaat
     7261 gcacacccaa tccaattgcc aatcggttct gaagatgact tccgtggtat cattgacttg
     7321 atcaagatga aagctgaaat ctatactaac gaccttggta cggatatcct tgaagaagac
     7381 atcccagctg aataccttga ccaagctcaa gaataccgtg aaaaattgat tgaagcagtt
     7441 gctgaaactg acgaagaatt gatgatgaaa tacctcgaag gtgaagaaat cactaacgaa
     7501 gaattgaaag ctggtatccg taaagcgact atcaacgttg aattcttccc agtattgtgt
     7561 ggttcagcct tcaaaaacaa aggtgttcaa ttgatgcttg atgcggttat cgactacctt
     7621 ccaagcccac ttgacatccc agcaatcaaa ggtattaacc cagatacaga cgctgaagaa
     7681 attcgtccag catctgacga agagccattt gcagctcttg ccttcaagat catgactgac
     7741 ccattcgtag gtcgtttgac attcttccgt gtttactcag gtgttcttca atcaggttca
     7801 tacgtattga atacttctaa aggtaaacgt gaacgtatcg gacgtatcct tcaaatgcac
     7861 gctaacagcc gtcaagaaat cgacactgtt tactcaggtg atatcgctgc tgccgttggt
     7921 ttgaaagata ctacaactgg tgactcattg acagatgaaa aagctaaaat catccttgag
     7981 tcaatcaacg ttccagaacc agttatccaa ttgatggttg agccaaaatc taaagctgac
     8041 caagacaaga tgggtatcgc ccttcaaaaa ttggctgaag aagatccaac attccgcgtt
     8101 gaaacaaacg ttgaaactgg tgaaacagtt atctcaggta tgggtgaact tcaccttgac
     8161 gtccttgttg atcgtatgcg tcgtgagttc aaagttgaag cgaacgtagg tgctcctcaa
     8221 gtatcttacc gtgaaacatt ccgcgcttct actcaagcac gtggattctt caaacgtcag
     8281 tctggtggta aaggtcaatt cggtgatgta tggattgaat ttactccaaa cgaagaaggt
     8341 aaaggattcg aattcgaaaa cgcaatcgtc ggtggtgtgg ttcctcgtga atttatccca
     8401 gcggttgaaa aaggtttggt agaatctatg gctaacggtg ttcttgcagg ttacccaatg
     8461 gttgacgtta aagctaagct ttatgatggt tcatatcacg atgtcgactc atctgaaact
     8521 gccttcaaga ttgcggcttc actttccctt aaagaagctg ctaaatcagc acaaccagct
     8581 atccttgaac caatgatgct tgtaacaatc actgttccag aagaaaacct tggtgatgtt
     8641 atgggtcacg taactgctcg tcgtggacgt gtagatggta tggaagcaca cggtaacagc
     8701 caaatcgttc gtgcttacgt tccacttgct gaaatgttcg gttacgcaac agttcttcgt
     8761 tctgcatctc aaggacgtgg tacattcatg atggtatttg accactacga agatgtacct
     8821 aagtcagtac aagaagaaat tattaagaaa aataaaggtg aagactaatc cgtcctcact
     8881 ctagaaggaa gtcacttagt ggcttccttt tgtctttaga aaatacctct aaatatggta
     8941 aaatagtaga agaataatgt gaggaaaatg aatgtcaaat agttttgaaa ttttgatgaa
     9001 tcaattgggg atgcctgctg aaatgagaca ggctcctgct ttagcacagg ccaatattga
     9061 gcgagttgtg gttcataaaa ttagtaaggt atgggagttt catttcgtat tttctaatat
     9121 tttaccgatt gaaatctttt tagaattaaa gaaaggtttg agcgaagaat tttctaagac
     9181 aggcaataaa gctgtttttg aaattaaggc tcggtctcaa gaattttcaa atcagctctt
     9241 gcagtcctac tatagggagg ctttctctga aggtccatgt gctagtcaag gttttaagtc
     9301 cctttatcaa aatttgcaag ttcgtgctga gggtaatcag ctatttattg aaggatctga
     9361 agcgattgat aaggaacatt ttaagaagaa tcatcttcct aatttagcca aacaacttga
     9421 aaagtttggt tttccaactt ttaactgtca agtcgagaag aatgatgtcc tgacccaaga
     9481 gcaggaagag gcctttcatg ctgaaaatga gcagattgtt caagctgcca atgaggaagc
     9541 gctccgtgct atggaacaac tggagcagat ggcacctcct ccagcggaag agaaaccagc
     9601 ctttgatttt caagcgaaaa aagctgcagc taaacccaag ctggataagg cggagattac
     9661 tcctatgatc gaagtgacga cagaggaaaa tcgtctggta tttgaagggg ttgtttttga
     9721 tgtggagcaa aaagtgacta gaacaggtcg tgttttaatc aactttaaaa tgacggacta
     9781 tacttcaagt ttttctatgc aaaagtgggt taaaaacgag gaagaggccc agaagtttga
     9841 cctcatcaag aagaattctt ggctccgagt tcgagggaat gtggagatga ataacttcac
     9901 acgcgatttg actatgaacg tacaggatct gcaggaagtt gttcactatg agcggaagga
     9961 tttgatgcca gaaggtgagc gtcgggttga gtttcatgct catactaaca tgtcgactat
    10021 ggatgctttg ccagaggtcg aagagattgt tgcaacagct gctaagtggg gacacaaggc
    10081 ggttgctatc acggaccatg ggaatgtcca gtcctttcca catggctata aggcggctaa
    10141 gaaagcggga atccagctga tctatgggat ggaagccaat atcgtggagg accgtgtccc
    10201 tatcgtctat aacgaagtgg agatggactt gtcagaagca acctacgtgg tctttgacgt
    10261 ggaaacgacg ggactttcag ctatctataa tgacttgatt caggttgcgg cttctaagat
    10321 gtacaagggg aatgttattg ctgaatttga tgaatttatc aatcctgggc atcccttgtc
    10381 agcctttact acagagttaa ctggaattac agatgatcat gtcaaaaatg ccaaaccact
    10441 agaacaagtt ttgcaagaat tccaagaatt ttgcaaggat acggtcctag ttgcccacaa
    10501 tgctaccttt gacgttggct ttatgaatgc taattatgag cggcatgatc ttccaaagat
    10561 tagtcagcca gttattgata cgctggagtt tgctagaaac ctctatcctg agtataaacg
    10621 ccatggtttg gggcctttga ccaagcgttt tggtgtggcc ttggaacatc accacatggc
    10681 caactacgat gcggaagcga ctggtcgtct gcttttcatc tttatcaaag aggtagcaga
    10741 aaaacatggt gtgaccgatt tagctagact caacattgat ctaatcagtc cagattctta
    10801 caaaaaagct cggatcaagc atgcgaccat ctatgtcaag aatcaggtag gtctaaaaaa
    10861 tatctttaag ctggtttcct tgtctaatac caagtatttt gaaggagtgc cacggattcc
    10921 gagaacggtt ctagatgccc atcgagaggg cttgatttta ggttcagcct gttcagaggg
    10981 tgaagttttt gacgtggtcg tttctcaagg tgtggatgcg gcggttgagg tggccaagta
    11041 ttatgatttt atcgaggtca tgccaccggc tatctatgca cccttgattg ccaaagagca
    11101 ggtcaaggat atggaggaac tccagaccat tatcaagagt ttgatagagg ttggagaccg
    11161 ccttggcaag cctgttctgg ctacgggaaa tgttcactat atcgaaccgg aagaagagat
    11221 ttatcgtgaa attatcgtcc gtagtttggg acagggtgcg atgattaatc gaactatcgg
    11281 tcatggtgaa catgcccaac cagcaccact tccaaaggct cattttcgaa cgactaatga
    11341 gatgttggat gaatttgcct ttttgggaga ggaactggct cgtaaactgg ttattgaaaa
    11401 caccaatgcc ttggcagaaa tatttgaatc cgttgaagtc gttaagggtg acttgtatac
    11461 gcctttcatc gacaaggctg aagaaacagt tgctgagttg acctataaga aagcttttga
    11521 gatttatgga aatccgctgc cagatattgt tgatttgcgg attgaaaaag aattaacatc
    11581 catactgggg aatggatttg ctgtgattta tctggcatcg cagatgctgg tgcaacgttc
    11641 taatgaacgg ggttatttgg ttggttctcg tgggtctgtc ggatctagtt tcgttgcgac
    11701 catgattggg attacggagg tcaatcctct ctctcctcac tatgtctgtg gtcagtgtca
    11761 gtacagtgag tttatcacag atggttcgta cggttcagga tttgatatgc cccataagga
    11821 ctgtccaaac tgtggtcaca aactcagtaa aaacggacag gatattccgt ttgagacctt
    11881 ccttggtttt gatggggata aggttcctga tattgacttg aacttctcgg gagaagatca
    11941 gcctagcgcc cacttggatg tgcgtgatat ctttggtgaa gaatatgcct tccgtgcggg
    12001 aacggttggt acggtagctg ccaagactgc ctatggattt gtcaagggtt acgagcgaga
    12061 ttatggcaag ttttatcgtg atgcagaagt agaacgcctc gctcaaggag cggcgggtgt
    12121 caagcggaca acaggccaac acccgggggg aatcgttgtt attccgaact acatggatgt
    12181 ctacgatttt acgcctgtcc agtatccagc agatgatgtc acggctgaat ggcagaccac
    12241 tcactttaac ttccacgata tcgatgagaa cgtcctcaaa ctcgatgtac tgggacatga
    12301 tgatccgacc atgattcgaa aacttcagga tttgtctggt attgacccta ataaaattcc
    12361 tatggatgac gaaggcgtga tggcactctt ttctgggact gatgtactag gggtaacacc
    12421 tgaacaaatt ggaacgccta cgggtatgtt ggggattcca gagtttggaa caaatttcgt
    12481 acgtggaatg gtagacgaaa cccatccgac aacctttgcg gaattgcttc agctgtctgg
    12541 tctgtcccac ggtactgacg tttggttggg gaatgctcag gatctgatta agcaaggaat
    12601 agcggaccta tcgactgtta tcggttgtcg ggacgacatc atggtttacc tcatgcatgc
    12661 gggtctggaa cctaagatgg cctttaccat tatggaacgg gtacgtaagg gtttgtggct
    12721 aaagatttca gaagaggaga gaaatggcta tatcgaagcc atgaaggcta ataaggtgcc
    12781 agagtggtat atcgaatcct gtgggaaaat taagtacatg ttccctaagg cccatgcggc
    12841 agcctacgtt atgatggcct tgcgtgtagc ttacttcaag gttcaccatc ctatttatta
    12901 ctactgtgct tacttctcca ttcgtgctaa ggcttttgat atcaagacca tgggtgcggg
    12961 cttggaggtc atcaagcgca gaatggaaga aatctctgaa aaacggaaga acaatgaagc
    13021 ctctaatgtg gaaatcgatc tctatacaac tcttgagatt gtcaatgaga tgtgggaacg
    13081 aggtttcaag tttggtaaat tagatctcta ccgtagtcag gcgacagagt tcctcatcga
    13141 cggggatacc cttatcccac catttgtagc aatggatggt ctgggagaga acgttgccaa
    13201 gcaactggtg cgggcgcgtg aagagggaga attcctctct aaaacagaac tacgcaagcg
    13261 tggtggactc tcatcaacct tggttgaaaa gatggatgag atgggtattc ttggaaatat
    13321 gccagaggat aaccagttga gtttgtttga tgagttgttt taaaaaattg cttaataatc
    13381 tattaaaaga ggctaacgta tatccaatag atttacatta gctttctttt ttgttaaaat
    13441 agtctatgga aagagggtga gagtatgtca aagatgagta taagcatccg tctggatagt
    13501 gaggttaagg agcaggccca acaggtgttt agtaatctgg gaatggatat gacaacagct
    13561 attaatattt tccttcgtca ggcaattcaa tatcagggat taccttttga tgttagacta
    13621 gacgaaaatc ggaagttgct ccaagcgtta acggatttag accaaaatcg taatatgagc
    13681 cagtcttttg aatcagtctc agatttgatg gaggacttac gtgcttaaga ttcgttatca
    13741 taaacagttt aaaaaagatt ttaagttggc tatgaagcgt ggtttgaagg cagaattatt
    13801 agaagaagtt ttgaattttc tggttcaaga aaaagaacat cctgccagat atcgtgatca
    13861 ttcattgacg gcatccaagc attttcaagg agttcgtgaa tgccataccc agccagattg
    13921 gcttttggtt tataaagtag acaagtcgga attgatttta aatttgctga ggacaggcag
    13981 tcacagtgat ttattttaat ctattttaag ggggttctca tgaaactaag aatatttgcg
    14041 gaagataagc cggctaagaa ggtatttgaa tatcaattag aacttgctga tcgtacaatt
    14101 cttctatcga cagcactctt gtcaggtgct attgctttag caggaatctt ttctgctttg
    14161 aaagaaaaat aaaaatagaa aagagaaaac agaatggttt taccaaattt taaagaaaat
    14221 ctaggaaaat atgcgaaatt gttggttgcg aacggaatta acgtgcaacc tggtcacact
    14281 ttggctctct ctattgatgt ggagcaacgt gaattggcac atctaatcgt gaaagaagct
    14341 tatgccttgg gtgcgcatga ggtcatcgtt cagtggacag atgatgtgat taaccgtgag
    14401 aaattcctcc atgccccgat ggagcgtttg gacaatgtgc cagaatacaa gattgctgag
    14461 atgaactatc ttttggagaa caaggctagc cgtcttgggg ttcgttcatc tgatccaggt
    14521 gccttgaacg gagtggacgc tgacaagctt tcagcttctg ctaaagctat gggacttgcc
    14581 atgaagccta tgcgtatcgc aactcaatct aacaaggtta gctggactgt agcagccgct
    14641 gctggacttg agtgggctaa gaaagtcttc ccaaatgctg cgagcgacga agaagcagtt
    14701 gatttccttt gggatcaaat cttcaaaact tgtcgtgtct acgaagcaga tcctgttaag
    14761 gcttgggagg aacatgcagc cattctcaag agcaaggccg atatgcttaa taaggagcaa
    14821 ttttcagccc ttcactacac agcgccagga acagatttaa cacttggttt gccaaagaac
    14881 cacgtttggg aatcagctgg tgctgtcaat gcacagggcg aagaattctt gccaaatatg
    14941 ccgacagaag aggtcttcac agcgcctgac ttccgtcgtg cagatggtta tgtcacttct
    15001 acaaaaccgc ttagctacaa cggaaatatc attgaaggca ttaaggtgac ctttaaggat
    15061 ggacaaatcg tagatatcac tgctgagaag ggtgatcagg ttatgaaaga ccttgtcttt
    15121 aaaaatgcgg gtgcgcgtgc cttgggtgaa tgtgccttgg taccagatcc aagtccaatt
    15181 tctcagtcag gcattacctt ttttaacacc cttttcgatg aaaatgcgtc aaaccacttg
    15241 gctatcggtg cagcctatgc gactagcgtt gttgatggag cggagatgag cgaagaggag
    15301 cttgaagctg cagggcttaa ccgttcagat gttcacgtag actttatgat tggttctaac
    15361 caaatggata tcgatggtat tcgtgaggat ggaacgcggg tacctctttt ccgtaatggg
    15421 aattgggcaa attaaggaga taatatgtta ggaagtatgt tcgttggtct cctagtggga
    15481 tttttagcag gtgctatgac caatcgtgga gagcgaatgg gatgttttgg aaaaatgttt
    15541 ctcggttgga tcggagcctt tctaggtcac ttgctctttg gaacttgggg gccagtttta
    15601 tcaggaacag ctattatccc agcgatttta ggagccatga ttgttttagc tattttttgg
    15661 agacgaggaa gttaatttcc taaatctgat actcaatgaa aatcaaagag caaactagga
    15721 aactagccgc aggttgctca aagcactgct ttgaggttgc agatagaact gacgaagtca
    15781 gtaacatata tacggcaagg cgacgttgac gcggtttgaa gagatttttg aagagtatga
    15841 aacgaaaagg aggttggtca ttgtactagc ctccttttga tatgatataa tagttctatg
    15901 agattagata aatttttagt tgcctgtgcg gtgggaagtc ggactgaggt caaaaacttg
    15961 ctcaaggctg ggcgcgtgac tgtaaatggt aaaaaagaaa aatcagctaa attgcagatt
    16021 gatgaaaaaa tagatgagat tcgctttgat gggcaagtgt tggagtatga agagtttgtc
    16081 tactacatga tgaacaagcc caaaggagtt atctcagcga ctgaggatcc caagcacaga
    16141 accgttctgg acttgttgga tgacttggcg cggagtaagg aagtttttcc agtaggacgc
    16201 ttggatattg acacgcatgg tcttttgctc ttgaccaata atggtcagct ggctcatgtt
    16261 cttctttcgc ccaagcgtca tgtggacaag acttatcagg ctcaggtcaa gggaattatg
    16321 acccaagaag atgtggagac atttatcaag ggcattccgc tcaaagactt tacctgtcag
    16381 cccgctagac tggagattct ttccacagat gcagaaaaga atcagagcca aatccgtgtg
    16441 accattgcag aagggaagtt tcatcagatc aagcgtatgg tgggctactg tggcaaggaa
    16501 gtggtggact tgcaacgatt gactatggga actttagtat tagatgagaa cctggaacga
    16561 ggggaatggc gtcgcttgac caaggaagaa ttagaaattc tccgcgctaa tattatttaa
    16621 tttagtttaa tactcaatga aaatcaaaga gcaaactagg aaactagccg caggctgtac
    16681 ttgagtacgg caaggcgacg ttgacgtggt ttgaagagat tttcgaagag tataaattcg
    16741 aaaaaggtga gggacaatgt cctcgccttt tatgtttttt agttgcttcc tttgtgaaaa
    16801 gagttataat agactgtaga ataaaaggag gaatctatga acgcgattca agaatcattt
    16861 actgataaac tatttgccaa ctatgaagca aatgtaaaat accaagcgat tgaaaatgct
    16921 gccagccaca acggaatttt tgcagctctg gaatgtcgcc aaagccatgt agacaacaca
    16981 cctgttttct cattggattt aaccaaggac aaggtcacta accagaaagc gtctggtcgt
    17041 tgctggatgt ttgcggctct caacaccttc cgccacaaac tcatctcgca atacaaattg
    17101 gagaactttg agttgtcaca agcccacact ttcttctggg ataagtatga gaaatcaaac
    17161 tggttcttgg agcaagtcat tgcgacttca gaccaagaat tgactagccg caaggttagc
    17221 ttcttactcc aaacacctca acaagatggc ggtcaatggg atatggtcgt ttccctcttt
    17281 gaaaaatacg gtgtcgtgcc taagtcagtt tatcctgagt ctgtttcatc tagcagcagt
    17341 cgtgagctaa atgcgatcct taataaattg cttcgtcaag atgctcaaat cttgcgtgat
    17401 ttgcttgttt ctggtgcaga tcaagcgact gttcaagcta agaaagaaga cctcttgcaa
    17461 gaaatcttta actttcttgc tatgtcatta ggacttccac cacgcaagtt tgactttgct
    17521 tatcgcgata aagataacaa ctataaaagt gaaaaaggaa tcacaccaca agagttttac
    17581 aagaaatatg tcaatcttcc tttagaagac tacgtttctg ttatcaatgc tccaactgct
    17641 gataaacctt acggaaaatc ttacacagtt gagatgttgg ggaatgtggt tggtagccgt
    17701 gcagttcgct acatcaacgt tccaatggag cgcttgaaag aattggcgat tgcccaaatg
    17761 caagcaggtg agactgtttg gtttggttct gatgtcggcc agctcagcaa ccgtaaggct
    17821 ggcatccttg cgacagatgt ttatgacttt gaatcaagca tggacattaa acttactcaa
    17881 gacaaggctg gacgtttgga ctatagcgaa agcttgatga cccacgccat ggtcttgaca
    17941 ggtgttgact tggacgaaaa tggtaaatca accaagtgga aggttgaaaa ctcatgggga
    18001 gacaaggtcg gtacagatgg ttactttgtt gcctcagacg cttggatgga cgaatacaca
    18061 taccaaatcg ttgttcgtaa ggaattgctg acagcagaag aacaagctgc ctatggagca
    18121 gaaccaatcg tacttgcacc atgggatcca atgggagcct tggctgaata aaagcataga
    18181 aaaaaggaat cagattttag aacctggttc ctttttagtt gcttgattac atgatgtgaa
    18241 gaacatgtgc cacaataccc actgcgaaga gggcaaggat aatagtgatt ggagatactt
    18301 ttttcttaag taagtacatg caaagtaaag taaggagtag tcctgataat ccaggaatca
    18361 acatatccaa gttttgttgg aaagtagtaa ctttttcagg agtttgagac aatccttgtc
    18421 ctacttgtgc gaatgcttct tggatacctt tagacccttc tggcaattta tcccaatgga
    18481 tataagcctt ttcatctagt tgaactttag aaacatcgaa agcaaattta atatttaccc
    18541 agcgttgaac aaggacagca agaatgaaca tcccaaggat agaagctcct ttagtgatat
    18601 cttgaaggat accaccagac atatctttag tgatttctga tccagccttg tatccaatct
    18661 cttgaacata ccacaagaat gacatacgaa tcaagttcca tgcaacaaag aagaggagtg
    18721 gccccaagat attgccagta agggcaagtg aagcaccgag agatccaagg attgggcgta
    18781 ctgtaaacca gaatactggg tcaccgatac cagcaagagg tcccatcata ccgattttaa
    18841 ccccttggat agcagcgtca tcgatttcca caccgttagc acgttcttct tcaagcgcaa
    18901 gagtaacccc catgactgga gcagctacgt atggatgagt gttgaagaac tcaaggtgac
    18961 gctcaagagc agcgatttga tcttcttttt tagtatagag ttttttgata gctggaatga
    19021 gtgtataagc ccagcccaag ttttgcatcc gttcaaagtt ccaagaccct tgtaagaagg
    19081 ttgaacgcca ccaaactttt ttacgatctg atttagttaa ttgaagtttt tcagtcatga
    19141 tgttttcagt cctttcttat cttagtagtc ttctaggata tcgccgattg ggtcgttaga
    19201 agttgcggct cctccgccac catttccacc agttttagaa aggtgaaggt agataagagc
    19261 gatagcaaca ccgatagcac cgaatccgat tagagtaata tctgacacag cagcaagaac
    19321 gaaaccaaga gcgaagaatg gccatacttc acgagttgcc atcatgttga taaccatggc
    19381 gtaaccaacg gcaacgacca taccaccacc gatagccata ccatctttga gccagtctgg
    19441 catggcacta aggatacttt gtacagtttc agttggtacc ataaggagaa gagctgcagg
    19501 aagcgcgata cgaagtcctt ggaaaagtag cgcaatgaaa tgagcacgct ccacagcgcc
    19561 gaagtcacct tttttagcgg cagcatcagc agtgtgaacc aatccaactg aaattgtacg
    19621 aacaatcata gttaagaata gaccagctac ggcaagaggg atagcaaccg cttgggcaac
    19681 accgatacca gtcttggtaa agtcaccacc aagaaccatg ataatggcag cagcgacaga
    19741 agcaagtgca gcatcaggag cgatagcagc accgatattt gaccaaccaa gggcaatcat
    19801 ttgaagcgat ccaccgagga taatccctgc ttccaagtga cctgttacaa gcccaataag
    19861 ggtacaggct acaagtggtt ggtgaaattg gaactggtcg aggatgcctt caagacctgc
    19921 aaagaaggct acaacgacta ctaaaaccat agaaataata gacatgttta aaatcctttc
    19981 ataaataatg gcttatttga cattggcttt gttaatcaag tcaaacaaat cttttttaga
    20041 atcatttggt actttacgga catcaaattc aacacccaag tcacgcattt tttcaaatgt
    20101 agcaacatct tctttgtcca tagacaaaac ggtattgacc aatgttttac ctgttgagtg
    20161 agccatagaa ccaacgttaa gagtcttgat tggcacgccg ccttcgatgg cacgaagggc
    20221 atcttgaggt gtttcaaaca agataagggc atgtgtttct ccaaaacgtg ggtcttttga
    20281 aatctcaatc agtttttgaa ttggaaccac gttagccttg acattacctg gagctgcttg
    20341 tttaatcaat tctttacgaa ggtcgtcttt agccacgtta tctgaagcaa cgatgatacg
    20401 atttgctttt gaatctggag tccaagcagt tgcaacctga ccgtgaagta gacgtgtgtc
    20461 aagacgggca agattgattt tcaatttacc gtctccgata acagttcctt ctgggatagc
    20521 agtttgggca actggagcag ctgcagcgct tgcaacttct tcaactggat ttagctcttc
    20581 tggaagagct ttgatgccat ctttggcttc tttaatgata ttagcagcga ctttttctac
    20641 acctgcagca gcgtccatga ggcgctctgt gtaggcttga atcaacatcg gtaagttaag
    20701 tcctgtgatg atggcaaact tacgctcagg attttctccc atcacgcgac tagcttggtt
    20761 aaatggagaa ccactccaaa ggtcagccaa aactagaacc tcatcttctg cgtcaaatgc
    20821 agcaacagcg ttattaaact tagcgtatag atcatcagga ccttcatttg gcataaaggt
    20881 cacaacttga accttttctt gttcaccaaa gatcatagat cctgactgat gaatacccgc
    20941 agcaaattcg ccgtggctcg caataatgat tccgatactc attattgtca ttcctccttt
    21001 tttgttttag ttttcattta agaaaacttt aagactgttt aagtataaac cgttttcata
    21061 aaaaaagcaa ctatttatca taaaaaatta aaaaattttt atttttgtat gttgatatgt
    21121 tgagtttttg aggagtaatc ttgaattctt atttaaaatt taatataaaa aagttggaag
    21181 aagcttccaa cttttaaaat aggtttctat taaattagtg ggtgaagtcg agtaccatac
    21241 gtccttggat ttggcctttt tccatttcgt cgaaaatggc aacagcatct tctactggac
    21301 gtttttgaac aactgggact accagacctt ctgcaccaaa ttggaaggct tcttctaagt
    21361 ctttacgagt tccgacaaga gaaccgatga cttggattcc atcgaggact gttttaacga
    21421 tgctgagttc catcatttca gaaggaagac caacagcgac gacgcgacca ccagcacgaa
    21481 tggagtcaac agcctggttg aaggcaactt tagacacagc agttacgaca gctgaatgag
    21541 ctcctccatc agttttttct ttaatgagtc cagctacatc ttcaacttcg aggccgttaa
    21601 tcacaatgtc agcgcctact tcttttgcaa gggtaagttt gtcattgttg atatcgactg
    21661 cgataacatg agcattgaat acttttttag cgtattgaac agcgaggtta ccaagtccac
    21721 cagcaccgta aagaacaacc cattggcctg gttcaacttt tgcttctttg atagctttat
    21781 aggttgttac tccagcacat gtgatagaag aagcttgggc tggatcaagt ccgtcaggaa
    21841 ctttgacagc atagtcagca gttacgatac attgttcagc cataccaccg tctactgagt
    21901 agccagcatt tttcactgta cggcaaaggg tttcgcgacc agttgtacag tattcgcaag
    21961 tgccacatcc ttcaaagaac caagcaacgc tgacgcggtc accgacttta aggcttttca
    22021 catctggagc aatctcttta acgataccga taccttcgtg cccaagaaca cgtcctggga
    22081 cttgaccaaa gtcaccatga gcaacgtgga ggtcggtgtg gcaaacgcca cagtattcaa
    22141 cttctacaag tgcttcccca gtttcaagtg gacggagtac tttttcttca atagcaacac
    22201 cagtgctttc tggatttaca acaacagctt tcatagctat cctccttgat attgaccgag
    22261 aacagggaca acgggactgg attgattttt tgtcaacaga gtcgagtgtg acgagctctc
    22321 ttgtatttat atgtgaagta tatcacaaaa ggaagccttt tccaaggttt tggagacaaa
    22381 aagtagaaca atcgattaac tggttgatga gactgtgaaa agagcacaaa gaccagcttg
    22441 caagctggtc atactcttcg aaaatctctt caaaccacgt cagcttcatc tacaacctca
    22501 aagcagtgct ttgagcaacc tacggctagc ttcctagttt gctctttgat tctcattgag
    22561 tatcagtata ggttataaga ataagtcttg tagggttttg gcaaccccat cttggtcgtt
    22621 ggtaagggaa atttgctcat ctgcataagg gagtagctct ggattggcat ttttcatggc
    22681 ataacccttc ccagcaaaag cgagcatttc ggtatcattg tgttcatctc caaaggcaat
    22741 caaatctttt ttgtcacgat tcattatctt gagcaagtag tccaaagcaa aggccttgtt
    22801 gacacctttt ggggtacatt caaggatatt gagcggacct ccccaggtat tgatagaaag
    22861 ttgatgctgg tagaaggcgt tcatttcttt tgccaaggca tatttgtcac tggctctggt
    22921 ctgcaagagg atacagttag ggtccttggt caccaattca ggctggaatt gatcttcagg
    22981 ctggaaagct tctacaccaa atagtttggg attggcaatt tcttcattgg gatttgtaat
    23041 gtagaatttt ttacgatatt ctccagcgat aaaatcggct tgaatgtcct ctgaacgttg
    23101 aaccatatct agcagatatt ttttgtctac agtcaaacac ttttcaaaat cccaaacttg
    23161 gtctggtaaa tgagtaaggg atccgttgaa gttaatcata ggagtgtcta agcccagttc
    23221 acggtaaaaa tcttttgaca tacggtaagg gcgacctgtc gtaataataa cctgatggcc
    23281 tttttcagca acttttttga tggttctttt ggtaaagtca gaaatttgac tgtctgagtt
    23341 gagcagggtt ccatccaggt caactgcaat aatttttttc gtcatatgga cctttctagt
    23401 gtgttttcag ataagatgtc ttctattata acagaaagca ggcagaaaag cagattccaa
    23461 acaaaaaatg aaattggatt aatttcttaa acaaatcaaa caaagacatt gaaaaagtga
    23521 atgataaatg atataattct attattgttc gtaaaaatta aaaggagatt gatgatggac
    23581 aaattattta aactaaaaga gaacggtaca gacgttcgta cagaggttct cgctggttta
    23641 acaactttct ttgcaatgag ctatattctc tttgtaaacc cacaaatact ttcacaaaca
    23701 ggaatgcctg ctcagggcgt cttcctagcg acgattattg gtgcagtagc gggtaccttg
    23761 atgatggctt tttatgctaa cttaccttat gcccaagcac caggtatggg actcaatgcc
    23821 ttctttacct ttacagttgt attcgggctt ggttattctt ggcaagaagc cctagctatg
    23881 gtcttcatct gtgggattat ttcattgatt attaccttga caaatgttcg taaaatgatc
    23941 attgaatcga ttcccaatgc tcttcgctca gctatttcag ctggtatcgg tgtcttcctt
    24001 gcctatgtag ggattaagaa tgctggactt ttgaaattca cgattgatcc aggcaactat
    24061 actgttgtag gagaaggggc tgacaaagct caagcaacga ttgcagcaaa ctcttcagca
    24121 gttccaggat tggtcagctt taataatcca gctgttttag tggctcttgc aggacttgcc
    24181 attactatct tctttgtcat caaagggatt aaagggggaa ttattctctc tatcttgaca
    24241 acaactgttc ttgctattgc agttggtttg gttgatttgt ctagtatcga ttttgctaat
    24301 aaccatgttg gtgcagcttt tgaagatttg aagacaatct ttggtgcagc tcttggttca
    24361 gaaggtttgg gagctttggt ttcagataca gctcgcttgc ctgaaactct gatggccatt
    24421 cttgccttct cattgacaga tatttttgac acaattggta ccttgatcgg tacaggtgaa
    24481 aaagttggta ttgtagcgac aaatggtgaa aatcaccaat cagccaaatt ggataaggct
    24541 ctttactctg atttgattgg aacgacagtc ggtgccattg caggtacttc aaacgtaacg
    24601 acttatgttg agtctgctgc tggtatcggt gcaggtggac gtactggttt gacagccttg
    24661 gttgtagcta tctgttttgc gatttcaagc ttctttagcc cacttctagc gatcgtacca
    24721 acagcggcta cagctccaat cttgattatc gttgggatta tgatgcttgg tagcttgaaa
    24781 aatatccatt gggatgatat gtctgaagca gttcctgcct tcttcacatc tatctttatg
    24841 ggattcagct actctatcac tcaagggatt gcagttggtt tcttgactta cactttgact
    24901 aagcttgtca aaggtcaagt taaagatgtt catgccatga tttggatttt ggatgccttg
    24961 tttatcctta actacatcag catggcctta taatagaatg acccaggggg atttcccccc
    25021 ttttttaata caaaggagat aggtgatgaa agagaaaaat atgtggaaag aattgttgaa
    25081 tcgtgcaggc tggattttgg tctttttact tgccgtcctt ttatatcagg ttcccctagt
    25141 ggttacctct attttgactt taaaagaagt agccctgcta cagtcagggc tgatagttac
    25201 tggcctttca attgtggttc tggctctatt tattatggga gctcgtaaaa ccaagttagc
    25261 tagttttaat ttttcttttt ttagagctaa agatttggca cgtttgggct tgagttattt
    25321 agttattgtc gggtcaaata tacttggttc tatcttgttg caactgtcaa atgagacgac
    25381 aacagctaac cagtctcaga ttaacgatat ggttcagaat agttctctga tttctagctt
    25441 cttcttgcta gccttgcttg ctccaatttg tgaggaaatc ttgtgccgtg ggattgttcc
    25501 taaaaagatt ttccgaggca aggagaactt gggatttgta gtcggtacga ttgtgtttgc
    25561 tttattgcat caaccaagta atttaccttc tttattgatt tatggaggta tgtcgacagt
    25621 tctatcttgg acggcctaca agacccaacg tttggaaatg tcgatcttgc ttcacatgat
    25681 tgttaatggg attgctttct gtttgttggc tcttgtggtg attatgagtc ggacattagg
    25741 aatttctgtg taaaagtttt tatgtaggaa ccgacctctt tctactaggg aaagatgaat
    25801 gcaatcgtgt ccatcttttt ctttttatgg taaaatagaa aaataatatg atgaaaatcc
    25861 ttgagggagt gaccgatatg tcaagtaaag ccaatcatgc aaagacagtt atttgcggaa
    25921 ttatcaatgt aaccccagac tccttttcgg acggtggtca attttttgct cttgagcagg
    25981 cactccagca ggctcgtaaa ttgatagcag aaggagccag tatgctcgat atcggcggag
    26041 aatcgactcg gccgggcagt agctatgttg agatagaaga ggaaatccag cgtgttgttc
    26101 cagtgatcaa agcgattcgc aaggaaagtg atgtccttat ttctatcgat acttggaaaa
    26161 gtcaggtggc agaggctgct ttggctgctg gtgccaatct agttaatgat atcactggtc
    26221 tcatgggcga tgaaaaaatg gcccatgtgg ttgctaaagc tggagcgaaa gtagtcatca
    26281 tgtttaatcc agtcatggct cgacctcaac atcctagttc gcttatcttc cctcattttg
    26341 gctttggtca aacctttaca gaaaaagagt tagctgactt tgaaacattg ccaatcgaag
    26401 acttgatggt ggctttcttt gaacgagcac tagcgagagc ggcagaagct ggtattacac
    26461 cagaaaatat cctgttggat ccaggaattg gctttggtct gaccaagaaa gaaaatctcc
    26521 tccttttacg ggacctggat aaactacatc agaagggcta tccaatcttt ctcggagtgt
    26581 cgcgcaagcg atttgtcatc aatatccttg aagaaagtgg gtttgaagtc aatcctgaga
    26641 cagagcttgg tttccgaaat cgggacacgg cttcggctca tgtaactagt atcgctgcga
    26701 gacagggtgt agaagtggtg cgcgtgcatg acgtagctag tcacaggatg gcagttgaaa
    26761 ttgcctctgc cattcgtctg gctgatgaag cggaaaattt agatttaaaa caatataaat
    26821 aagatgaaag aaattgaaaa caatcagtgg attgctaact accggacgga tcaaccgcat
    26881 tttggattgg aacgaatggt ggaactgtta gctttgcgtg gcaatcccca tctcaaactc
    26941 aaggtcctcc atatcggagg gactaacggc aagggctcga ctattgcttt tttgaaaaag
    27001 atgctagaaa agctagggct gagagttggc gtgtttagct cgccctatct cattcattac
    27061 acagaccaga ttagcatcaa tggggaatcg atctcagaag cgaggctaga agctctcatg
    27121 gcagactatc agtctttgct ggagggagaa gcggtcgcca atttacaggg cacaaccgag
    27181 tttgagatta tcacagccct ggcctatgac tactttgcct cagagcaagt agatgtggcc
    27241 atcatggaag ttggcatggg tggacttttg gatagtacca atgtctgtca gcccattttg
    27301 acaggaatta caactattgg cttggatcat gtggccctac ttggtgacac cttggaggcc
    27361 atagcagagc agaaggcagg tattatcaaa caagggatac ccttggtaac agggcgtatt
    27421 gctccagaag ccttgactgt gattgaccgc attgcggaag ggaaagatgc gccgagactt
    27481 gcctacggga cagattatca ggttcgtcat caagaaagtg tggtgacagg ggaagtcttt
    27541 gactatacaa gtgctgtcag acaaggtcgc ttccagacta gcctgcttgg tttgtaccaa
    27601 atagagaatg ctgggatggc catagcttta cttgatactt tttgtcaaga agatggtcga
    27661 gagctagcaa gcaatgactt tcttggtcaa gccttggaag aaacaagttg gccagggcgt
    27721 ttggaaatcg tgtcaagaga tcccttgatg attttggatg gagcccataa tccccatgct
    27781 atcaaggcct tgttggtaac cttgcaagaa cgttttgcgg attatcataa ggaaatcctc
    27841 ttcacttgta tcaaaaccaa agccttggag gatatgttgg acttgctggg agccatgcca
    27901 gataccgagc ttactctaac acattttgcg gatagtcggg cgacggatga aagcgtgctg
    27961 aaagaggcag ctaagtctag aaatctcagc taccaagatt ggcatgattt tctagagcag
    28021 aatttgacag ataaaaaaga agagaaacaa acagttagga ttgtcacagg ttccttgtat
    28081 ttcttgagcc aagtgagggc ctatctgatg gagaggaaga acgagaatgg atacacaaaa
    28141 gattgaagca gctgtaaaaa tgattatcga ggctgtagga gagaacgcta atcgcgaggg
    28201 cttgcaggaa acacctgctc gtgtagcccg tatgtatcaa gagatttttt caggtcttgg
    28261 tcaaacagca gaggaacatt tgtcaaaatc ctttgaaatt attgacgata atatggtggt
    28321 agaaaaggat atctttttcc ataccatgtg tgaacaccac ttcttgccat tttatggtag
    28381 agcgcacatt gcctacattc cagatggtcg tgtggcaggc ttgtctaagc tagcccgtac
    28441 ggttgaagtt tattcgaaaa aaccacaaat tcaagaacgt ttgaatatcg aagtggccga
    28501 tgccttgatg gactatctag gtgctaaagg agcctttgtt gtcattgagg cggaacatat
    28561 gtgtatgagt atgcgtggtg ttagaaaacc aggcactgca accttgacga cagtagctcg
    28621 tggtctattt gaaacagata aggacctccg cgaccaagct tatcgtttaa tggggctata
    28681 aaaagaatcc gcttcaagcg gatttttcta gaaaggaatc attatggatc aactgcagat
    28741 taaggatttg gaaatgtttg cctatcatgg tctttttcct agtgagaaag aattggggca
    28801 gaaatttgtc gtttcagcca tcctatccta tgatatgacc aaggcagcta cagacttgga
    28861 tttaacagcc tctgtccatt acggagaatt atgtcagcag tggacgactt ggtttcagga
    28921 aacgagtgaa gatttgattg aaacggtagc ctataaactg gtggaacgta cctttgagtt
    28981 ttatcctctt gtccaagaaa tgaagttgga actgaaaaaa ccttgggcac cggtgcattt
    29041 gtcactagat acttgctcgg taaccattca tcgccgcaag caacgagcct ttatcgccct
    29101 aggaagcaat atgggagata aacaagcaaa cttgaagcaa gccattgaca aactgcgagc
    29161 tcgtggcatc tatattctca aagagtccag tgtcttagcg acggagcctt ggggtggagt
    29221 ggagcaggat agctttgcca atcaagtggt tgaggtggaa acctggctac cagcacaaga
    29281 cttgttagaa accttgttag ccattgagtc agagctggga cgggtgagag aagtgcattg
    29341 gggacctcgt ttgattgatt tggacttgct ctttgtggag gaccagatcc tttatacaga
    29401 cgacctcata ttgcctcatc cttacatagc ggaacgcctt tttgtccttg agtccttaca
    29461 ggaaattgcg cctcatttta tccatccgac attaaaacaa ccgatccgca acttgtatga
    29521 tgctttgaaa aaatagaaaa actctagttt tcagttactt gcaactgaag gctagagttt
    29581 ttaaaatagg tcattttctt ctgggaggag gatagtttct ctaccgtaca tgtctaaaac
    29641 cagtactctt gggggataac gagggtcgaa aggatggtta aagtcaaaat caatggctgt
    29701 agggaggtgt tgacttgaaa agtggaaggt aatctttcct tggttattaa gcaattgaaa
    29761 ctcgagttct tcttccaatt caaagacatt ttttaagaaa tgatcgatga tataccaaaa
    29821 agagtcaatg atgtcatcgg gcaagctggt aacaatgcca aaactagcag accgcatgtg
    29881 ggtattggta aaagccatat ctctgtcccc tttcttttcc cttatcatac agcaaatagg
    29941 attaaaaatc aagaaaaggt gattttttga aaaggatttt aactacaggg aaaaataagg
    30001 aaaaaaattc ccaaaaatct accgaagtta ataggtaaat tcccaaatta acttgattat
    30061 ataactttca gtta
//
LOCUS       DAVBBS010000022        29758 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVBBS010000022 DAVBBS010000000
VERSION     DAVBBS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29758
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.022"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            132..494
                     /locus_tag="VQU32_000958"
     CDS             132..494
                     /locus_tag="VQU32_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472429.1"
                     /translation="MRLASESRQILDKLKDDVYNKLGYEVSYSSIVSQAVREYVPKKE
                     RIDWIKLKETAIPFSSLKQSNNWEYQTSLMLEKDVLILLSELQNFFLDVFQAKRIHRA
                     FCIRLCLRAQFLLSNNDS"
     gene            902..1201
                     /locus_tag="VQU32_000959"
     CDS             902..1201
                     /locus_tag="VQU32_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472430.1"
                     /translation="MNRNVLNFLRTESAERVSLYIDKANRLEGDVTLLAPSSQDLEDI
                     KNAMFSNPNLELKVARLDVMKKIAYASTRNHYLTGATIFGDISKGTYNCDPKSYV"
     gene            complement(1296..1733)
                     /locus_tag="VQU32_000960"
     CDS             complement(1296..1733)
                     /locus_tag="VQU32_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472431.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISNTYTILSISSFLV"
     gene            complement(1864..2498)
                     /locus_tag="VQU32_000961"
                     /pseudo
     CDS             complement(1864..2498)
                     /locus_tag="VQU32_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
     gene            complement(2709..3359)
                     /locus_tag="VQU32_000962"
     CDS             complement(2709..3359)
                     /locus_tag="VQU32_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET2472432.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRMHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            3471..4313
                     /locus_tag="VQU32_000963"
     CDS             3471..4313
                     /locus_tag="VQU32_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET2472433.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            4389..4826
                     /locus_tag="VQU32_000964"
     CDS             4389..4826
                     /locus_tag="VQU32_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET2472434.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKKSKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTDIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            4894..5196
                     /locus_tag="VQU32_000965"
     CDS             4894..5196
                     /locus_tag="VQU32_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472435.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(5287..6360)
                     /locus_tag="VQU32_000966"
     CDS             complement(5287..6360)
                     /locus_tag="VQU32_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HET2472436.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDKLAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHKIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            6516..7193
                     /locus_tag="VQU32_000967"
     CDS             6516..7193
                     /locus_tag="VQU32_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2472437.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            7186..8160
                     /locus_tag="VQU32_000968"
     CDS             7186..8160
                     /locus_tag="VQU32_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2472438.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVEMQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQEICIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(8209..10152)
                     /gene="thrS"
                     /locus_tag="VQU32_000969"
     CDS             complement(8209..10152)
                     /gene="thrS"
                     /locus_tag="VQU32_000969"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET2472439.1"
                     /translation="MINITFPDGAIREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHGDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(10210..10386)
                     /locus_tag="VQU32_000970"
                     /pseudo
     CDS             complement(10210..10386)
                     /locus_tag="VQU32_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
     gene            complement(10393..10533)
                     /locus_tag="VQU32_000971"
     CDS             complement(10393..10533)
                     /locus_tag="VQU32_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HET2472440.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            complement(10964..11164)
                     /locus_tag="VQU32_000972"
     CDS             complement(10964..11164)
                     /locus_tag="VQU32_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472441.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKGLYINNLL
                     FFTPMVILIVSLLYSYRFRFSL"
     gene            11403..11948
                     /locus_tag="VQU32_000973"
     CDS             11403..11948
                     /locus_tag="VQU32_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET2472442.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGSGS
                     LYIDETKTTRLLDTPDFPEEFKSLAKAQADELDLLRTKNNLNWAFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKRNKPD"
     gene            12174..12443
                     /gene="rpsO"
                     /locus_tag="VQU32_000974"
     CDS             12174..12443
                     /gene="rpsO"
                     /locus_tag="VQU32_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET2472443.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            13259..13873
                     /locus_tag="VQU32_000975"
     CDS             13259..13873
                     /locus_tag="VQU32_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HET2472444.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(14165..14914)
                     /locus_tag="VQU32_000976"
     CDS             complement(14165..14914)
                     /locus_tag="VQU32_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET2472445.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            15047..17383
                     /locus_tag="VQU32_000977"
     CDS             15047..17383
                     /locus_tag="VQU32_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET2472446.1"
                     /translation="MDKNKIMGLTQREIKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAVASILTSYMA
                     HSEDKNPTAQAIRQRFQGQVVYPMLSNLPFSSDRKWGAMELEGLGIVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLNY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELIALAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            17485..18162
                     /locus_tag="VQU32_000978"
     CDS             17485..18162
                     /locus_tag="VQU32_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HET2472447.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            18149..18946
                     /locus_tag="VQU32_000979"
     CDS             18149..18946
                     /locus_tag="VQU32_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET2472448.1"
                     /translation="MLASEVIHAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            18963..20066
                     /locus_tag="VQU32_000980"
     CDS             18963..20066
                     /locus_tag="VQU32_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET2472449.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLVQDMEAYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPDLTGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            20073..20264
                     /locus_tag="VQU32_000981"
     CDS             20073..20264
                     /locus_tag="VQU32_000981"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472450.1"
                     /translation="MKIKEQTRKLAAGYSKHCFEVVDRTDEVSSKHCFEVVDRTDEVS
                     NHTHGKATLTWFELDFRRV"
     gene            20367..21386
                     /gene="galE"
                     /locus_tag="VQU32_000982"
     CDS             20367..21386
                     /gene="galE"
                     /locus_tag="VQU32_000982"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET2472451.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAIEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            21388..22341
                     /locus_tag="VQU32_000983"
     CDS             21388..22341
                     /locus_tag="VQU32_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2472452.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRKLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDDRLKKIEFI"
     gene            22493..23158
                     /locus_tag="VQU32_000984"
     CDS             22493..23158
                     /locus_tag="VQU32_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472453.1"
                     /translation="MLSKEDYTEEIGLIKKQNYVEAELYPIVADIIKPTLKDSLSKRY
                     VFGRQRRGLGQIYYGLSNFPDIVILDKTYENKSRKSIKIEEWKKLRGCVEVKNLNYSL
                     ITEEKIKSTISNSFEHITGEMEQLTGEMGQLIGDLLWYKKVIYTNGIEWRFLSLDDKE
                     EIDNTIVEVVNKRIETEEAGNSFDWWKNIKDLSFNYTDICLSKDCRQEWNEFVKRVKE
                     IEW"
     gene            23197..23298
                     /locus_tag="VQU32_000985"
                     /pseudo
     CDS             23197..23298
                     /locus_tag="VQU32_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group 2 glycosyl transferase"
     gene            complement(23349..23555)
                     /locus_tag="VQU32_000986"
     CDS             complement(23349..23555)
                     /locus_tag="VQU32_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET2472454.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            23607..24083
                     /locus_tag="VQU32_000987"
     CDS             23607..24083
                     /locus_tag="VQU32_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET2472455.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            24092..24763
                     /locus_tag="VQU32_000988"
     CDS             24092..24763
                     /locus_tag="VQU32_000988"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET2472456.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDFETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            24894..25232
                     /locus_tag="VQU32_000989"
     CDS             24894..25232
                     /locus_tag="VQU32_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HET2472457.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLADGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            25235..25864
                     /locus_tag="VQU32_000990"
     CDS             25235..25864
                     /locus_tag="VQU32_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HET2472458.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            26104..27255
                     /locus_tag="VQU32_000991"
     CDS             26104..27255
                     /locus_tag="VQU32_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2472459.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            27568..28317
                     /gene="truA"
                     /locus_tag="VQU32_000992"
     CDS             27568..28317
                     /gene="truA"
                     /locus_tag="VQU32_000992"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET2472460.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQAI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFTVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            28307..29080
                     /locus_tag="VQU32_000993"
     CDS             28307..29080
                     /locus_tag="VQU32_000993"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET2472461.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            29058..29519
                     /locus_tag="VQU32_000994"
     CDS             29058..29519
                     /locus_tag="VQU32_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2472462.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT         8719 a         5366 c         6692 g         8981 t
ORIGIN      
        1 taaattttta gattgtggaa tttttttcaa cttaattctt gatttttttt ctaaattgtt
       61 atatacttaa tttacgggat aacagaatcc cgtcagaaag gggtgataat atgggaaaag
      121 agaatgttag aatgcgttta gcgtctgaat ccagacagat attagataag cttaaagatg
      181 atgtttacaa taaattaggc tatgaagtaa gctattccag tattgttagt caagctgtta
      241 gagaatacgt tcctaaaaaa gaacgtatag attggataaa attaaaagag acagctatac
      301 ctttttcatc gttaaaacag agcaataatt gggagtacca gacatcactt atgttagaga
      361 aagatgtatt aatcttactg tctgaactac aaaatttttt cttagatgtc ttccaagcga
      421 aacgtataca tagggcattt tgtatacgtc tatgtttgag agcgcaattt ttattaagta
      481 ataatgattc ttaatactat caatattaat gcggagtgta atgctccgca aaaataaaac
      541 ctattacggg ataaaaacac tactttattt aggatggaaa taagaacgta ttttttacta
      601 atattgttta gaaaggagtt tctttatgac accagaagaa caaactgctt tggaatactt
      661 ttatgaggaa ctcgagagtt acggtgaaga tgagcaagga gaggtctttg atttgtatga
      721 taattaaatc atctccatag tttctgagaa taattgagaa actatactct aaatcatagg
      781 tttatgtaaa gctagatata cacaaaagga ggtacgaatg aagatgaaag ctagagcttc
      841 accgttgaat tgattttgtt tctagaaaaa atcaataatt agaaaaatac gaggtaaaaa
      901 aatgaataga aatgtactaa attttctgcg aacagaaagt gctgaaagag ttagtcttta
      961 tatagacaag gctaatagat tggagggcga tgtaactcta ctcgcaccta gttctcaaga
     1021 tttggaagat attaaaaatg ctatgttttc caatcctaat ctcgagctaa aagtagcgag
     1081 actggatgtg atgaagaaaa tagcatatgc ttctacaaga aatcattatt taacaggggc
     1141 tacgattttt ggggatattt cgaaaggaac atacaattgt gatcctaaaa gttatgtcta
     1201 atataaggga ggttaatccc tccccttgtt tttgaaaatc gaaaggaaaa gtataaaaat
     1261 gaaagttaat gaatatttct ggagaacgag ctgtcttata ctaaaaaaga agaaatcgat
     1321 aaaatagtat acgtgttaga gatgtgtttg tcgtaaaagt ctggatagtt ttctcttagg
     1381 tagtcagctg tcatataata aaaagtagaa tggcaagcag tgatgattgg cataagagat
     1441 gaaaaacgct gagaactaat gaaggctaag agaacaaaaa atagaaaatc aatggtgaga
     1501 acccctaaat agtagaagcg aatcttttta ttgatttgag gataaatctc agcgaattga
     1561 tgtttgagtc ggacgaccaa gagaaatagc aggagtcctt gagcaaggaa gaaaattaaa
     1621 ccagagagca agagccactc aaaagatgtc tcgagtctag tgataaaaaa tgcaaatagt
     1681 agcaaatcaa taactgcaat tgctataaaa tggattctat agagtttttt catgacaaga
     1741 cctccctctt ttatctaact tcattctact ctaaaagaat gggagttaca actaaaatga
     1801 taaaaatagc agaggggaga ttctcttaag ttggctagta ttctttattt gagtttcctt
     1861 ctattatcta acttcttcat cattccagac aaataaagct ccgattgcat tgaggatata
     1921 aaagatgtat ttaccgatat tggcgaagtt tccttgaata ccagcttttg tcagctgaac
     1981 gaaattgtaa atcaaccaaa agccccactg tccctcattg ccaatgcatt caaagcattg
     2041 gcaatgaggg acagtgcaaa ggcaatagtt gttacgtagg caaggagatt catcttgccc
     2101 ccatatccga tatagttggt cacaaaggca aagaggaagg cgatgatgga aatgatgatg
     2161 gccgccaatt ttacctgttt ttggctcatt tggttgggtc tgccttcttg cgaagctttc
     2221 catttcttta tagcaaaggt ataaatgagg aaggtgacgg gataggtaat gatggccgcc
     2281 ttatttccaa ggatataatc aatagcaccg gacaaaatgg tattaacaat accaaagtaa
     2341 ttttcccatt tgcttaattt ccccgtgaaa cgagtggaca acatggaaat cccaacgttg
     2401 gttacggaaa tcaatccaaa gggtacaaga gctgtccatg gtccccagtc tacaaattta
     2461 tcgaggcgtg agttgaggta accagatgca atcgcaatcc aacgaccaaa gcaaccccga
     2521 agaggtcaaa ctatttagat gtagcaaaaa tttttagtga ttttttcata ggttaaacta
     2581 cctttctttt tttcaaatat tctcccattt ttttttcaaa tattctacca ccaaatgaaa
     2641 gtaaaataaa atgatagaaa taaaaccctg aaaataaaac tttcagggtt ggtaggggac
     2701 ttgagaaatt agttgatttt ctcgacatag agttgttttg caatgtccat attcacttgt
     2761 aaatcctcgt cgttactgag gatagtgaag gtattgctga agccatcaaa ctgcttaact
     2821 tggagctggt caccgatgtg aagtgagtgc ttgtccagat aatggagaat gtcaaaacta
     2881 tcgtgcatcc gagtcaggcg gtaggcgcca gcttccttga tatcagctag tgggaggtta
     2941 ttgatttcaa cgaggagttc tcccttggca ggaatagttc ctccgtgggg gcaggtttta
     3001 gggaaaccta gcagtttatc tagtctttcc acgaacaggt cagagacagt gtgttccaag
     3061 acctcagctt cctcgtgaat ctggtcactt gtatagtcta aatgatgaac tagaaaaact
     3121 tcaatcaagc ggtgcttacg atagagctca gagaccagtt tgagaccgag gtcagtcagt
     3181 agatagccac attccttgtc ctttaggatg agattttcac ttttcattcg tttgatcatt
     3241 tcagttacgg cagggggaga gacttgcatg cgagccgcaa tttccttgtt ggtaatctta
     3301 tgcaggtcta tgccaatttc ataaatacat tttagatagt cttctttgtt tggggtcatt
     3361 cgtttcctct tgtctaatat ctttatctag tatatcaaaa aaagctagat ttctctagct
     3421 gtgacttttt atgttatact tattgcaaga gaaaaaacga ggagatggat atgacgaaaa
     3481 tagctcttct ttcagatatt catggaaata ccaccgcctt ggaggctgtt ttggcagatg
     3541 ctcggcagct aggagtggat gaatactggc ttttgggaga tattctcatg ccagggacag
     3601 ggcgtagaag gattttggac ttgttggatc aactaccgat tacggctaga gttttgggaa
     3661 actgggaaga cagtctttgg catggtgtcc gtaaggaatt ggatagtact cgccctagtc
     3721 aacgctatct cttgcgccag tgccagtatg ttttagagga aatttcccta gaagaaattg
     3781 aagtgctcca caatcaacct cttcaaattc atcgtcagtt tggggatttg acagtgggaa
     3841 ttagccatca tctgcctgat aagaactggg ggcgagagtt gattcatact ggcaaacaag
     3901 aggaatttga ccgcttggtg actcatcctc cctgtgatat tgctgtttat ggacacattc
     3961 accagcagtt gcttcgttac gggactggtg ggcaattgat tgtcaatccg ggttcgattg
     4021 gccaaccttt ctttttggac gctcagctac ggaaggactt gcgggcccag tatatgattt
     4081 tggagtttga tgacaagggc ctggtagata tggacttccg acgggtagat tacgatgtgg
     4141 cagctgaatt gcagctggct aaagacctga gacttcccta ttttgaggtt tactatgaaa
     4201 gtctggtcaa tgggatccac catactcatc atcaggaatt tttgagagaa ttggctcaga
     4261 aagagggttg cgatcgggag ttagacgact ggttgaaaag tggtaacgat tgacattaca
     4321 actaaaaagg gtataataat agaaaaagaa gagtagaggc agggtagcct cgtaaaaagg
     4381 agaagaggat gcaaattcca agtagattta ccattgcgac tcatatgctg ataatcattg
     4441 ccctcgaggg gaagaaaagc aaggtgacca gtgattttct ggctgctagt gtcggggtca
     4501 atcctgtcat tatcagaaag atcttgtccc agttgaagaa ggcagagctg atttcagtag
     4561 cgcgtggaac gggcggaaca gatattgtca aggaccttaa ggatattagt cttttagatg
     4621 tttatcaggc ggtcgaatgt cttggtaaga caggtcaact cttcagtttc catgacaatc
     4681 cgaatccaaa ttgccctgta ggagctcata ttcatgatgt tttggatcaa aaattggaga
     4741 gaattcagtt gactatggag gcagaacttg gtcaaaccag tctagaaaaa gtcgtggccg
     4801 atgcagagag tcagatgaag gattaagagg gataaatgcc ctcttttttc tttggataat
     4861 tatgataaaa tgtagctatt aaaggagtaa gaaatgtatc tcgttatagg aattgtatta
     4921 gcctttatag tgtcattttg gaaggataat agaagtttgt ggaatccagt attattttta
     4981 ttatccctca tttcaagtta tttttatcta tcctaccttt tttataaaaa tggatacgag
     5041 aatgttcagt tagcttttta tgtatttgcc tttgtcttac ttcctttttt gctttttcta
     5101 agtggcatct ttttaatcta taatggggtc attttgttga agcgagaggg acgttccaag
     5161 ccccattatc tctcaatgct ctttgatttt tattgactat tagattctat ttaaaaagac
     5221 ttagaatgca catgaaagta tgtattctag gtcttttgtt ttgtatacat ttaattttca
     5281 agagtattat ctatttttcc atgcctggta acgagtatca atcaataact gggtaaggcg
     5341 acccaaggtt tctctttctt ctggagtgag ggattgagct tggacaaaga gatgatgaat
     5401 gtattcaagc gcctttaagg aagccaagtc attgatagag ctaataccag cctcaagaat
     5461 agtgccaaag aagaggtcga agtagagctg aagttcttcg tcagggactg tggccaccca
     5521 ttctttaaag gttgtgtcta cttgctggct atcactgttg gtcttatcca gttggacgaa
     5581 gtgcttgtcc tcaatctgcc aactaaaggt atcgtgctgg gcgatgccac ccagggcagt
     5641 actctgaacg atgattttgt gagcaggaat ttccagcatc ataccgataa tggaaccttg
     5701 tggaatgaat atcttgcttc tatccattat cctttgataa cccgcagtct gtgtcaattc
     5761 ttgatggaga ccaggtgcat caaatgtata aactgctgtg atctgatttt gcaaactttg
     5821 ctcaatttgg ctagcagcat agatagcgag atttcctccc ttggaatgcc cagctagaat
     5881 aaccttttgc ttaggatgat gggcaaaaaa gttctttaaa tagcgaaggg cgtgcttttg
     5941 agcaggaatt tccttcatat aggtcaggtg gaaatcttcc ttccagccaa tgatactgtc
     6001 atctgtccca cgaaagacaa tcagataggt atcgaggctg acacgataag tcatagccgc
     6061 aaattgcttt tgcagttcag ggtcgatgtc gttgataaaa tgggagagtt tgcaattttt
     6121 gaagcgcttg tgttgagcca atttatctaa taattgaagg cgatttttgc tagtaagcat
     6181 ggtgggctct cttggaacct gaggtgctag atctaaaaga cgttgaggaa gtgtggagac
     6241 cagattatca aaggagaggt aggtgatttc tattaaggtt agaatgtcta actcattcaa
     6301 gggaaggtcg taataagaat catatgcgac atctttcaga tagtcaaaaa tattggccat
     6361 aagagctcct ttctttttat ttatcttagc aaatttccct caaattagct agtagcatag
     6421 cctgtttgca ctggctaaaa acaggctatt tcaaattcag tttcagacca tctagcatgg
     6481 aaaaatctgt tataataatg gaaaaggaga agcgcatgca caagatttta ttaatagaag
     6541 atgatcaggt cattcgtcaa cagattggga aaatgctctc tgaatgggga tttgaagtgg
     6601 tcctggtaga agactttatg gaagttttga gtctatttgt tcagtcggaa cctcatctgg
     6661 tcctcatgga tattggtttg cccttgttta atggttatca ctggtgtcag gaaatccgca
     6721 agatttccaa ggtacctatc atgtttcttt cttcgagaga ccaggctatg gatattgtca
     6781 tggcaatcaa tatgggggcg gatgactttg tgaccaagcc ttttgaccag caggttcttt
     6841 tagctaaggt tcagggcttg ttgcgtcgtt cctatgagtt tgggcgtgat gagagtttgc
     6901 tggaatatgc tggtgttatc ctcaatacca aatccatgga tttacattat caagggcaag
     6961 tcttgaattt gaccaagaat gaattccaga ttttacgcgt gttatttgag catgcaggca
     7021 acatcgtagc acgtgacgac ctgatgcggg aactttggaa cagtgacttt ttcattgatg
     7081 ataataccct ctctgtcaat gtggctcgtt tgcgtaaaaa gttggaggag cagggattgg
     7141 taggatttat cgagaccaag aaaggaatag ggtacggatt gaagcatgct tgattggaaa
     7201 caattttttc tagcctatct gcgctcccgt agtcgtcttt ttatctatct gctttctttg
     7261 gcatttcttg tcttactctt tcagttttta tttgccagtc taggaattta cttcctctac
     7321 tttttcttct tgtgttgctt tgtaaccatc ttatttttca cttgggacat attggtggaa
     7381 atgcaggtct atcgccagga acttctctat ggagagaggg aagccaagtc tcctttggaa
     7441 atagctttag cagaaaaatt agaagcgcgt gagatggaac tctatcagca gaggtcaaaa
     7501 gcagaaagaa aactgacgga tttgctggat tactatacct tgtgggtcca tcagataaag
     7561 acccccattg cagccagtca actcttagtt gcagaagtgg tcgaccgcca actgaagcag
     7621 cagctagaac aggaaatttt caaaatcgac tcttatacca acctagtttt acagtacctg
     7681 cgtttagaaa gtttccatga tgatttgctc ttaaagcagg ttcaaattga ggacttggtc
     7741 aaggaaataa ttcgtaaata tgctcttttc tttattcaaa aaggcttaaa tgtcaatcta
     7801 catgaccttg ataaagaaat cgtgacggat aaaaagtggc tgctagtggt tattgagcaa
     7861 atcatctcaa acagtctcaa gtacaccaag gaaggtggtc tggagattta tatggatgac
     7921 caagagattt gtatcaaaga tacgggaatc gggataaaaa acagtgatgt cctccgagta
     7981 tttgaacgtg gcttttcagg atacaatggc cgtttgaccc agcagtcctc tggacttggc
     8041 ctttatctat ctaagaaaat ttctgaagaa ctggggcacc agattcgtat cgagtctgag
     8101 gtcggaaaag gaacgacagt gcggattcag tttgctcaag tgaacttagt ccttgagtaa
     8161 aaatagatga gaaaaagcct ccagattgga ggcttttatg ctagactctt atttctcaac
     8221 gcgtgatttg ttggcgatat cagctaggat agcttgaaca aaattatcaa ctgagacagt
     8281 ttgtgtttct ttttggccgt agcgacgaac gttgactgtt tcgtcttcca tttctttgtc
     8341 tccaacaatt aattggtaag gaatcttgct ggtttgtgaa gcacggatct tgaactgcat
     8401 tttttcattg cgctcatcta cgtctgcacg gacaccgcgg tcacggagtt tcttggctac
     8461 ttcccaagcg tagtccacgt gtttttcgtt agatactggg atgagggtta cttggtgtgg
     8521 tgccagccat gttgggaagg cccccttgta gttctcaatc aagatagctg tgaagcgttc
     8581 catagttgag ataaccccac ggtggatcat gactggacgg tgatcttcgc catcagctcc
     8641 gatgtatttg aggtcgaagc gttctggcaa caagaaatca agttggatag tagaaagggt
     8701 ttcttctttt ccaagggcag ttttaatctg gatatccaat tttggtccgt agaaggctgc
     8761 ttcaccttcg gcttcaaagt agtccacgcc catttcatca agagctgcac gaagcatggt
     8821 ttgggcattt tcccacatct catcgttatc aaagtactta tgagtatctt gagggtcacg
     8881 aagagagagg cggaagcggt agtcagtcaa gttgaagtct tcataaacat cgataatcaa
     8941 ctgaagggca cgttggaatt cttcttggat ttgttctgga gtaacgaata ggtgaccgtc
     9001 gttgagtgac atttcacgta cacgttgaag gccagtgagg gcaccagatt tttcgtaacg
     9061 gtgcatcata ccgatttcag cgatacggat tggcaattca cggtaagagt gaacatggtg
     9121 tttgaaaact tggatgtggt gcggacagtt cattggacga aggacaaatt cttccccgtc
     9181 acccatgtcc atggttggga acatgtcttc ttggtaatga tcccagtgac cagaagtctt
     9241 gtaaagctca acagaagcaa gtggtggagt gtagacgtgt tggtagccag aagccaactc
     9301 tttgtttacg atgtagcgtt ccaattcacg acggatagtc gcaccatttg gcaaccagaa
     9361 tggcaaacct tgtcccactt cttgtgaaat cataaagagg tcaagctctt taccaagttt
     9421 acggtggtca cgttccttag cttcttcacg catttgaagg tagtttttca agtctttctt
     9481 gtcaaaccaa gctgtaccgt agatacgttg catcatagcg ttgtcgctgt ttccacgcca
     9541 gtacgcacca gctacatgga gaaggtggaa gatttggata cgacctgttg atggaacgtg
     9601 aggtccacgg cagaggtcta catattcacc ctgacgatag atagtcaaac cgccttcgtc
     9661 ttctgagtgt tcttcaatca attccaactt gtaagggtca tttttgaaga tttcacgtgc
     9721 ctcgtcttta gtcacttctt cacgaataga tgggaagttt tctttgacga ttttttgcat
     9781 ttcttcttcg atacgaggaa ggtcttcgtt agagatttga ccagctgtgt tgtcagtatc
     9841 gtagtagaaa ccatcttcga tggctggacc aactcccaag tgaatgtctg ggaaaagacg
     9901 acgagctgct tgggcgaaca agtgagctgc tgagtgacgc aagattggaa gggcatctcc
     9961 gtgatcaggt gtcacaattt cgatgcttcc atcttcagtg atagcgcgag tagtgtcgat
    10021 gagtttgccg ttgaatttac cagccaaggc ttttttagct agggaattgc tgatagattg
    10081 ggcaatttca aaagttgtta cgccagattc gaattcacga atagcgccat ctgggaaagt
    10141 aatgttaatc atgatgttct ccttttttat tatttcatag tttctaagca ttgatttttt
    10201 atcagcagac tacttccaag ttctaacgtt caattgcttt tctttatcag agaggaagtt
    10261 ttccatttcc gtcagaatat ctatatccaa acgttgagaa agttctaata gtcaccagat
    10321 attttctgaa agtttttgtt ccagtgtgta gggtgtttca tcatagtagc gtccttgctt
    10381 tgtcatcacc agtcattgga aatttccaat atcattagat aaagccaaga ggtcttcttc
    10441 taccgtccac ttgaaatcat gatgcttaac ttccagttcg tgataagctt gtcggattgc
    10501 ccaactgcgc tccactaatt cttgcaactc cataattagt atcctaataa aaaaatgcga
    10561 catcctcgaa aggacgtcgc aacgtggttc caccttcatt tatgtacctc aaaagagagg
    10621 gacacctctg atcggctcta acgtggccac cgttttatgt tttcataaaa aactcaagag
    10681 tagtatcagt ttagacttcc tatgcgtttc cagcaaccac gcactctcta gtagaaaggg
    10741 actaagtgac ttgtctctat ggattattat agcatgattg tgagattttt caaggatttt
    10801 gtgaaagttt tttgtttttc attttttgta gaatatgctt gatgctaata ccagaatcca
    10861 tgcccaccag agcaactgtt tcatagatta gcaagtaaat tagtataaac acaccaaagg
    10921 ttatgatagt aaagtaaaag agcagtccgt taccaactaa ccatcaaagt gagaaacgga
    10981 aacggtagct atagagtaac gatacaatca agattaccat aggtgtaaag aagagaagat
    11041 tgtttatata gagtcccttg agtgtaaggg ggtcagaaac aactagcaag aacaactcat
    11101 aaagtggcca ataaaagaat aatatgacca gatagtaggg gagatgggaa tagaatctac
    11161 gcatgataat cacctcgttt taaaaaaaca gacaacttag atctcttgct atgattatta
    11221 tagtatgttt aaaacaagaa agtaaatttg ttgacaaaga agatttttct tttcactaga
    11281 atcatggtta gttttcttgg tagaaatatt ttgataataa ttaagcaaga ctattgacat
    11341 tgattattaa gagtgtcaca attgttgtaa ctaaaataat tacaacaaga ggggtataaa
    11401 aaatgaaact agcagttatt gcagcaaatg gacaagcggg gaaggcaatt gttgaagaag
    11461 cagtcaaacg aggacatgaa gtaacggcta ttgttcgttc tgaaaacaaa agtcaagctg
    11521 agtctattat taaaaaagat ttatttgagt tgacaaaaga tgatcttact ggatttgatg
    11581 ctgttatttc agcgtttggt gcttatacac cagatacgct accgctccac agcaaatcaa
    11641 tcgaactatt taatcagctt cttgctggaa cacagacacg ttttctagtt gtagggggat
    11701 caggaagcct ttatatagat gaaacaaaaa cgacacgact tttagatacg cctgattttc
    11761 ctgaagaatt taagtcacta gcaaaagctc aagcggatga attggatctt cttcgtacaa
    11821 aaaataatct caactgggct tttgtcagtc ctgctgtaga ttttattccc gatggtgaaa
    11881 aaacaggaaa ctacattcta gcaggagaaa tttttactac taatgaaaaa aggaataagc
    11941 cagattagtt atgcagatta tgctattgga ttggtagatg aacttgaaaa aggtcatcat
    12001 ataaaagaac gtatctcttt acttgaaaaa taataactat gagagtatta atgtttgaca
    12061 gaaaatttaa atcttggtag aatagggatt gtcgtaaaga caaataactt cttcttggtt
    12121 acaggcatgc caacctgtca ctcggatgaa gccaaataaa aaggagaaac atcatggcaa
    12181 tctcaaaaga gaaaaaaaat gaaatcatcg cacaatatgc acgtcacgaa ggtgatacag
    12241 gttcagtaga ggttcaagtt gctgtcctta cttgggaaat caaccacctt aatgaacaca
    12301 tcaaacaaca caaaaaagac cacgctactt accgtggatt gatgaaaaaa atcggtcgcc
    12361 gtcgtaactt gcttgcatac ttgcgtaaaa acgacgttaa ccgttaccgt gagttgatca
    12421 actctctagg acttcgtcgc taattcaaga tacaaaggct gtcaaaagca caaagtaaaa
    12481 ataggaaaat ttacgaagaa acttcagttt ctaggagatt ttatcttttt tgccaagtgc
    12541 ttaggccgtg ttcaattgag catatcttga taatgaagct actctaaatt gggtagcttt
    12601 tttgtgttgt caccttacct cgatatactc aagtataatc tttggttacg gttcctagca
    12661 ctgtaaggta aaataaacca gcttttctcc cttcggggag attttttttg tttcactgaa
    12721 agtttcgtac gatcttcgcc tcgtcgccta gtctataaac gatttatcta gcaagaatca
    12781 tgatgctaag ggcgtcaaaa atccgtatga aaatagggaa atgacacagt gttcgatgag
    12841 cacaaggagt ttcatctttt tcactaggat tttagcccgg actcaaatca gctctctgac
    12901 ttcagagggc ttttttattg aggttttatc ggtaacaatt ttggagacta caaaaacctc
    12961 aaacggtatc agctaattac accataaagg tgcgcagtta ctcggctttt caagccgagt
    13021 agttgtatgc aagcccccta gaagagccca ctctttacaa agtaaagtat agtatgttat
    13081 actttacatg gaagtagtca ctgaattcca gttagaaatt actttgtaac tacgttttga
    13141 ggaggagtaa aatgctttcc tacgttcgat attacccact agcgatagct aaattaatgt
    13201 gtctgtgctc tcctaaaatc tgctgattta ttactgacta atacaggagt ttttttttat
    13261 gggacagaca atcatatctg ctattggtgt ttatatttcc accagtatcg attatttaat
    13321 tattttaatt attttatttg cacagctatc acagaataaa cagaaatggc atatttatgc
    13381 ggggcaatat ctaggcacag gcttacttgt aggggcgagt ttagttgctg cttatgtcgt
    13441 taatttcgtg cctgaagaat ggatggttgg attgcttggt ttaatcccta tctatttagg
    13501 gattcgcttt gcaattgttg gagaagatgc ggaagaagaa gaggaagaaa ttattgaaag
    13561 attagaacaa agcaaggcaa atcaactgtt ttggacagtt acattgctga caattgcgtc
    13621 tggcggagat aatttaggta tctatatacc ttattttgct tcgttagatt ggtcacagac
    13681 cctcgtggcc ttgcttgtgt ttgtaatcgg cataattatc ttttgcgaga ttagtcgggt
    13741 gttatcttct attccgttaa tattcgagac aattgaaaaa tacgagcgaa tcattgtgcc
    13801 cttagtattc attctacttg gactatacat catgtatgaa aatggcacga tagagacttt
    13861 tctgatcgtg tagatttttt tgtttcacta ggattttagt ccgagctcaa atcagctctc
    13921 tgattttcag agggcttttt cattgagtat aaatggacaa tcttaaagtt actctattgt
    13981 agagtggctt ttgatttgat taggagattc ttataaatat agtaaaatga aataagaata
    14041 ggacaaatcg atcaggacag tcaaatcgat ttctaaaaat gttttagaag tagaggtgta
    14101 ctattctagt ttcaatttga tatataaatg aaaaccttag ctgtttggcc aaggttcatt
    14161 tcttctatgc aagttcttct cttttcttat ctgggttcca gatgaagctt gcgataaagc
    14221 taaagatgat ggttaggata gcgagaataa tagcaaggat gagggcaggg atgcggataa
    14281 accaccagag tggatttggt tttttatcat tgtgccattg tttcgtttct tcgtccagac
    14341 gttggaattc tgtttgaata cgattggcga ctgtttcaat gccttcatca ttcattttct
    14401 tgatatctga gatatcgatt ggatttccaa agttcatatc gacacgttca cggctaatca
    14461 agcccttcaa agtcatggga ccggtgtagg taaccggcat gatacggacc ttggccattt
    14521 tggcaatcag tgctgcgccc cccttgacat cgtttgagtg gcggctacca cttggaaaca
    14581 tgatgagaga gcggtcactt tttttgagaa cgttgatagg atatttgatg gctgaggcgc
    14641 tgggattttc acggtcgatg ggaaaggcgc cacacatacg aatccaccaa ccaaagatac
    14701 ggttggtaaa gagttctttt tttgccataa agatgaactg ttttggcttg gtcgcaaagg
    14761 ccatataaac aggatcccac caggtacggt gaggcgcaac taaaatataa ttttcatctt
    14821 gattaggaat tttatcagta ttatgatagt gagcattgcc attgatggac cataagagca
    14881 atacaactaa tccacgcaaa taagtataaa acatgcgatc tccttcgatt gttttcttgt
    14941 tattattata ccttatcaaa ggagggctgg caaacttttc ccttgactag atgcatattt
    15001 gggatgaaat tagaattctg ttaaaaaaaa tgatataata gaatttatgg ataaaaataa
    15061 gattatggga ttaacccaaa gagaaatcaa ggaaagacag gctgagggtt tggtcaatga
    15121 ctttaccgca tcagccagta ccagcacttg gcaaatcgtt aaacgaaatg tctttaccct
    15181 ttttaacgct ttgaactttg ccattgcttt ggcccttgcc tttgtgcagg cttggagcaa
    15241 tctggtcttc tttgctgtta tctgctttaa cgctttttct gggattgtga ccgagctacg
    15301 agccaaacac atggtggaca agctcaatct catgaccaag gaaaaggtca aaaccatccg
    15361 tgatggtcag gaagttgctc ttaatcctga agaattagtg ctaggagatg tcattcgttt
    15421 gtctgcagga gagcagattc ctagtgatgc cttggttttg gaaggctttg cggaagtcaa
    15481 tgaagccatg ttaacgggag aaagtgattt ggtgcaaaag gaagttgacg gcttactttt
    15541 gtcaggaagt ttcctagcca gtgggtcagt tttatctcaa gttcaccatg tcggtgcaga
    15601 caactatgct gccaaactca tgcttgaggc taagaccgtt aaacccatca actcccgtat
    15661 catgaaatcg ctggacaagt tggctggttt tactgggaag attatcattc cctttggtct
    15721 ggctctcttg ctggaagcct tgcttttaaa aggcctgcct ctcaagtcat ccgttgtaaa
    15781 ctcgtcgaca gctcttttgg gaatgttgcc taagggaatt gcccttttga ccattacttc
    15841 gctcttgact gcagtgatta agttgggctt gaaaaaggtc ttggtgcagg agatgtactc
    15901 tgttgagacc ttggcgcgcg tggatatgct ctgtctggac aagacgggta ccatcaccca
    15961 aggaaagatg caggtggagg ctgttcttcc actgacggaa acttatggtg aagaggctgt
    16021 tgccagcatc ctgaccagct atatggccca tagtgaggat aaaaatccaa cagctcaagc
    16081 cattcgccag agattccaag gtcaagtagt ctatcctatg ctctctaatc ttcccttctc
    16141 cagcgaccgc aagtggggag ccatggagtt agaaggtttg ggaatagttt tcttaggggc
    16201 acctgagatg ttgctggatt ctgaggtccc agaagccaga gaggccttgg agagaggatc
    16261 acgtgtcttg gtcttagctc tcagtcagga gaaattagac catcacaaac cacagaaacc
    16321 atctgatatt caggctctag ccttgctgga aatcttggat cctattcgag agggagcggc
    16381 agagacgctg aactatctcc gttctcagga agtggggctc aagattatct ctggtgacaa
    16441 tccagttacg gtatccagca ttgcccagaa ggctggtttt gcggactatc acagctatgt
    16501 cgattgttca aaaatcacgg atgaggaatt gattgctctg gcggaggaga cagcgatttt
    16561 cggacgtgtt tcccctcatc aaaagaaact cattatccaa acgctgaaaa aagcgggtca
    16621 tacaacagct atgactgggg atggagttaa tgatatcctg gctcttcgtg aggcggattg
    16681 ttctatcgta atggcggagg gagacccggc aacccgtcag attgccaacc tggttctctt
    16741 gaactcagac tttaatgatg ttcctgagat tctttttgaa ggtcgtcgtg tggtcaataa
    16801 cattgcccac atcgctccga ttttcttgat aaagaccatc tattccttcc tgttagcagt
    16861 catctgtatt gccagtgctt tactaggtcg gtcagagtgg attttgattt tccccttcat
    16921 tccgatccag attaccatga ttgaccagtt tgtggaaggt ttcccaccat tcgttctgac
    16981 ttttgagcga aatatcaaac ctgttgagcc aaatttcctc agaaaatcca tgcttcgtgc
    17041 cctaccaagt gccctcatgg tcgtgtttag cgttcttttt gtgaaaatct ttggaacgag
    17101 tcaaggttgg tctgagttag aaatctcaac tctcctctat tatctcttgg ggtcaattgg
    17161 tttcttatcc gtatttagag cctgcatgcc attcactcta tggcgagtcc tcttgattgt
    17221 ctggtcagta ggaggcttcc tagccacagc tctcttccca agaatccaaa aactgcttga
    17281 aatttcaacc ttgacagaac aaacgttgcc tgtttatggt gtcatgatgt tggtctttac
    17341 agtgattttc atcctgacta gtcgctatca agctagaaaa taaagaaaga ctgcaatctg
    17401 tggattgcag tttttttagg tacaagattg ccagccgaaa tgtggtataa taagaggtaa
    17461 tagagttttg gaaagtgaga gaagatgatt tcaaagagat tagaattggt agcttccttt
    17521 gtgtcacagg gggctatttt actagatgtg ggaagtgacc atgcttatct gcctatcgag
    17581 ttggttgaga gaggccaaat caaaagcgct attgcaggtg aggtggtgga aggtccctat
    17641 cagtctgcgg ttaaaaatgt tgaggctcac ggcctaaagg agaaaatcca agtccgttta
    17701 gccaatggct tggcagcttt tgaagagact gaccaagtgt ctgtcattac cattgctggc
    17761 atgggtggtc gtttgattgc taggatttta gaagaaggtt tggggaagtt agctaatgta
    17821 gagcgtttga tcctccagcc caataatcgt gaagacgact tgcgtatctg gctacaggat
    17881 catggattcc agattgtagc agaaagcatc ttagaagaag ctggaaagtt ttatgagatt
    17941 ttggtggtgg aagcaggaca aatgaagcta tcagccagtg atgttcgctt tggtcccttc
    18001 ttgtccaaag aagtcagtcc agtatttgtc caaaaatggc aaaaagaagc tgagaagcta
    18061 gagttcgctc tcggacaaat cccagaaaaa aatctggaag aacgtcaagt tctagtagat
    18121 aagattcaag ctatcaagga ggtgctccat gttagcaagt gaagtgattc atgcttatga
    18181 agccttttgc cctcaggaat tttctatgga gggagacagc cgtggtctgc aaattggcac
    18241 tttagacaag ggtatccaaa gggtcatggt tgccctcgat attcgtgaag agacagtggc
    18301 agaggccatt gaaaagggtg tggacttgat tatcgtcaag cacgcgccta tctttcgtcc
    18361 tatcaaggac ttgcttgcca gccgtccgca aaatcagatt tacatcgacc tgattaagca
    18421 tgacatcgca gtttatgtca gccataccaa tattgatatc gttgaaaatg ggctcaatga
    18481 ctggttttgt cagatgctag gaatcgagga gacgacttat ctgcaggaaa caggtccaga
    18541 acgtggaatt ggacgtattg ggaatattca gcctcagaca ttttgggaat tggcccaaca
    18601 ggtcaagcaa gtctttgacc tagatagcct tcgaatggtg cattatcaag agaatgattt
    18661 gcagaagcct atttcaagag tagcaatttg tggtggaagc gggcagtctt tctataagga
    18721 tgctttggca aagggggcag atgtctatat cactggtgat atctactacc atactgctca
    18781 ggatatgctg tctgatggct tgttagcatt ggatccaggt cactatatcg aagtgctttt
    18841 tgtggaaaaa atcgcagcac tccttactca atggaaagag aaaaaaggct gggaactaga
    18901 gattttacct agtcaagcat cgaccaatcc tttccgtcat atctagttag aaagtgaaga
    18961 caatgaaaaa agttgccatt attggagcag gaattgtggg agcaacagct gcctactacc
    19021 tctcgagaga aagtgaccta gaggtgaccg tttttgacca tgagcaaggt caagccacca
    19081 aggccgcagc aggaattatc agtccttggt tttccaaacg ccgtaataaa gcctggtaca
    19141 agatggcgcg cttgggggct gatttttatg tggatttatt agctgattta gagaaatcag
    19201 gacaaaaaat cgacttttac cagcgttcgg gagtctttct cttgaaaaag gatgaatcca
    19261 agttggaaga actttatcaa ctggccctcc agcgcagaga agaatctccc ttgatagggc
    19321 aattagccat tctggaccaa gcctcagcta atgaattatt ccctggtttg cagggatttg
    19381 accgcctgct ctatgcttct ggtggagcga gagtagatgg ccaactcttg gtaactcgtc
    19441 tgctagaagc cagtcatgtc aagctggtca aagaaaaagt gactctgaca ccgttagcat
    19501 caggctacca gattggtgaa gaggagtttg agcaggttat tttggcgacg ggagcttggt
    19561 tgggggacat gttagaaccc ttgggttatg aagtagatgt tcgtcctcaa aaaggacaac
    19621 tacgagatta tcagctggtc caagacatgg aagcttaccc tgttgtcatg ccagaagggg
    19681 agtgggattt gattcctttt gcaggtggga aattgtcctt aggcgctacc cacgaaaatg
    19741 acatgggatt tgatttgacg gtagatgaaa ccttgctcca acaaatggag gaggccacct
    19801 tgactcacta tctgattttg gctgaagcta cttcaaaatc tgagcgtgtt ggaatccgtg
    19861 cctacaccag tgatttctct cctttctttg ggcaggtgcc tgacttaact ggtgtctatg
    19921 cagccagtgg actaggttca tcaggcctca caactggtcc tattattggt taccatttag
    19981 ctcaactgat ccaagacaag gagttgacct tggaccctga aaactaccca attgaaaact
    20041 atgtcaaacg agtaaaaagc gaataatact caatgaaaat caaagaacaa actaggaaac
    20101 tagccgcggg ttactcaaag cactgctttg aggttgtgga tagaactgac gaagtcagct
    20161 caaaacactg ttttgaggtt gtagatagaa ctgacgaagt cagtaaccat acccatggca
    20221 aggcgacgtt gacgtggttt gaattggatt ttcgaagagt ataagcattt tactgaaatt
    20281 ttatcaggta gtttaggatg gcaaatgaca ttccctgtca aaaatggtaa aataagaaaa
    20341 aataatctga gaatcgagga aaaaagatgc aagaaaagat tttggtaact ggtggtgccg
    20401 gttttattgg aacccacact gttattgaat tgatccaagc aggccatcaa gttgttgtgg
    20461 tggataatct tgtcaatagc aatcgtaaga gtttagaagt tgttgaaaga atcacaggag
    20521 ttgagattcc tttctatgag gcagatattc gtgacacagc taccctcaga gatattttca
    20581 agcaggaaga accgactggt gtcattcact ttgctggttt gaaggctgtt ggcgaatcaa
    20641 cacgtatccc tcttgcctac tatgacaaca atatcgctgg aactgtcagc cttttgaaag
    20701 ccatagaaga aaacaactgt aaaaacatca tcttcagttc ttctgcgaca gtttacgggg
    20761 atccgcacac agtgcccatc ttggaagatt tcccactttc agtgaccaac ccatacggtc
    20821 gtactaagct catgctagag gaaattttga ctgatattta caaagcagac tcagaatgga
    20881 atgttgtctt gcttcgttac tttaacccaa tcggagccca tgagagtggt gatttgggag
    20941 aaaatccaaa cggtattcca aacaatctct tgccatatgt gactcaagta gccgttggaa
    21001 aattagagca agtgcaagtg tttggagacg attacgatac ggaagatgga acaggtgttc
    21061 gtgactatat ccacgttgtc gatttggcta agggtcacgt tgcagctttg aaaaaaatcc
    21121 aaaaaggttc aggactaaac gtttataacc ttggaactgg taaaggttac tcagttcttg
    21181 aaattatcca aaacatggaa aaagcggtgg gacgtcctat tccttaccgt atcgtagaac
    21241 gtcgcccagg tgatatcgct gcctgctact cagacccagc aaaagctaaa gcagaactcg
    21301 gttgggaagc agaactcgac atcacccaaa tgtgtgaaga cgcatggcgt tggcaaagca
    21361 agcatccaaa tggatttgaa gactaagatg atgatttcaa tcatcgtccc ttgtttaaac
    21421 gaagaggaag tacttcctct tttttatcag gctctggaag ctttacttcc agatttggaa
    21481 acagaaatcg agtatgtctt tgtcgatgat ggatcaagtg atgggacctt ggaactctta
    21541 aaggcctatc gggagcaaaa tccggcagtc cattatattt ctttctctcg aaattttggc
    21601 aaagaagcag ccctttatgc aggcttgcaa tatgcgacag gagatttggt ggtggtgatg
    21661 gatgcagacc tccaagatcc tcctagtatg ttgtttgaga tgaaaaatgt actagacaaa
    21721 aatgtagact tggactgcgt tgggacacgg agaactagtc gggagggaga acccttcttt
    21781 cgcagtttct gtgctgttct cttttatcgc ctcatgcaaa aaatcagccc agtggctctg
    21841 ccgtcgggtg tccgtgattt tcgtatgatg agaaggtctg tagtcgatgc cattttaagc
    21901 ttgaccgagt ccaatcgttt ttcaaagggg ctcttcgcct gggtgggttt caaaacctac
    21961 tatttggact atccaaatgt cgaaaggcag gctggcaaga ccagttggag ttttaggaaa
    22021 ctctttttct actccattga agggattatt aatttttcag attttccctt gattatagcc
    22081 tttgtagctg gtctcctatc ttgttttctt tctctgctga tgaccttttt tgttgtggtt
    22141 cggaccctca ttttgggcaa tccgacatct ggttggacct ctctgatggc tgttattctc
    22201 tttcttggag gcattcaact cttgaccatt gggattctcg gcaagtatat cagtaagatt
    22261 tatttagaga ctaaaaaaag accactttat cttatcaaag aaaaaagtga tgataggcta
    22321 aaaaagatag aatttatctg acatagtcac tttttgttat ttgtaattct tttagttcgg
    22381 agtttgggtg atttttaaaa gaattaggga tttgaaatga tttaaaaaat ctatgtagac
    22441 taaatttaaa ttgtagtgac ttaataaata aaaaatttag gagtattatg ttatgctaag
    22501 taaagaggat tatactgaag aaatcgggct aataaagaaa caaaattatg tagaggctga
    22561 attgtatcct atagttgctg atataattaa gccaacttta aaagatagtt tatcaaaaag
    22621 atatgtcttc ggaagacaaa gaaggggttt gggacaaatt tattatggtc taagtaattt
    22681 tcctgatata gttattttag ataagactta tgaaaacaaa tctaggaaaa gtataaaaat
    22741 tgaagaatgg aagaaacttc gaggatgcgt ggaagttaag aatttaaatt atagtttaat
    22801 tacagaggaa aaaatcaaat caaccatatc gaacagtttt gaacatataa ctggagagat
    22861 ggaacaacta actggagaga tgggacaact tattggagac cttttatggt ataaaaaagt
    22921 aatttataca aatggaattg aatggagatt tctaagttta gatgacaagg aagagataga
    22981 taatacaatt gttgaagtag ttaacaaaag aatagaaaca gaagaagcgg gaaattcttt
    23041 tgattggtgg aagaatatta aagatttatc gttcaattat acggatatat gcctatctaa
    23101 agattgtaga caagagtgga atgaatttgt aaaaagggta aaggaaatag aatggtagaa
    23161 gtctattctc attgagaaat ttagatttta agttacagta agatttattt agagactaaa
    23221 aaaagaccac tttatcttat caaagaaaaa agtgaccttc ctgattttac agaaaaaaat
    23281 aaagtgaaaa gactataatt ttacatggaa atgtgctaaa ctagagggag tggattatcg
    23341 ccattgattt aggcttcctg gtttccaatc agggcgcgag ttgggcaatt tttaacagcc
    23401 tctaagacat cctgactagg agaaatttct ttttccagtt ggtcagggtc atcgtaaaaa
    23461 cgcacgattc cattatcgtg gtaatcaaat aaatcagaat aagtttggca aagcccacag
    23521 gcgatacatc gttcaggtat aagtgtgatt ttcatgttca tatttatatt gtaataagaa
    23581 aagtaaaaaa aaacaaggag taaggtatgg caaaagaacc gtggcaagaa gatatctatg
    23641 atcaagaaga atcaagagca gagcgtcggc atcgaaacca cggaggggct gataggatgg
    23701 ctaatcgtat tttgacgatc ctagctagta ttttctttgt aattgtggtg gtgatggtca
    23761 tcgttctcat ctatctatca tcggggggaa gtaatcgcac agcagcctta aaagactttc
    23821 atgattctga tgcaagtgta gtacaaatct catcttcaag cagttctcag cctgagcaga
    23881 gttcagagcc agaatctact tctagtagtt cagaagaagc tgctaatcct gaaggaacga
    23941 ttaaagttct cgcaggagaa ggggaagcag ctattgccgc tcgtgcagga atctccattg
    24001 ctcagttaga ggccttgaat cctgggcaca tggctacagg atcttggttt gctaatccag
    24061 gtgatgttat aaaaataaaa taggagccga tatgaaaaca attcaaattg ctattgatgg
    24121 tcctgcttcc agcggtaaga gtacggtcgc aaagattatt gctaaggatt ttggattcac
    24181 ctaccttgat acaggagcta tgtatcgtgc agcgacctat atggctctta agaaccaatt
    24241 aggagttgaa gaagtcgaag cccttctagc cttgttggac cagcatccaa tcagctttgg
    24301 acgttcagaa actggagacc agcttgtttt tgtaggagat gtggatatta cccatcctat
    24361 ccgtgaaaat gaagtgacca atcatgtttc tgctattgca gcaattcctg aagtgcgtga
    24421 gaaactggtt tctctccaac aagaaattgc ccagcaaggc gggattgtca tggatggtcg
    24481 cgatattgga actgttgtat tgccacaagc agaattgaaa attttcctag tagcttctgt
    24541 tgatgagaga gcagagcgtc gttacaagga aaatattgcc aagggaattg aaacagactt
    24601 tgaaacccta aaaaaggaaa ttgctgcgcg tgactacaag gatagtcatc gtgagacttc
    24661 tcctctcaaa caagcagagg atgctgtcta ccttgataca actggtttga acattcaaga
    24721 agtagttgaa aaaatcaaag cagaagctga aaaaagaatg taaatgtaga aaagccgata
    24781 gattgatatc ggtttttttc taaatttgtc aaaatgctga ttttagggta taatagttgc
    24841 tgttcgagaa attttgattt tcaaagaata gtgaatgatg ataaggagaa accatgaaca
    24901 atttaccaaa ttgtccaaaa tgtaactcag agtatgtcta cgaagacggt gccctactgg
    24961 tttgcccaga gtgtgctcat gagtggaatc ctgctgaagt tgcagaagta gaagaaggtc
    25021 ttgtcgctat cgatgccaac ggaaataaat tggctgatgg tgatacagta actctcatca
    25081 aggacttgaa agtaaaaggt gcgccaaaag atttgaaaca agggacgcgc gtgaaaaata
    25141 tccgcatcgt agaaggcgac cacaatatcg actgtaagat tgatggtttt ggtgccatga
    25201 aacttaaatc agagtttgtg aggaagattt aagcatgtca aaacactata aacttgtatt
    25261 ttatagccgt atcttcttgt ttctagcggc ttttacggga gtttatcttg aaattactaa
    25321 gcatggtggt tttgggatgc ttctctatta cacggttctg tccaacctct tggtaactat
    25381 ttttaccctt tatcttctaa aggttatgag ccgtgtaggt gaaaattggc aaaggccaag
    25441 tctcttgcgc ttaaaaggtg gggtcaccat gagtatcatg attacctgtg tgatttacca
    25501 tttcctcttg gcgcccattg cgactaattt ctatacccta gaaaatttcc tttgccacta
    25561 tatcgttccc atctggtttt tagcggatac cctctttttt gacaaacagg gtcaatacaa
    25621 gatttgggat ccagcagtgt ggaccatttt accctttctg tatatgatgt ttgctctttt
    25681 taatggcttg gttctaaaac tcaatattcc aaatgccaag gataatcctt tcccttactt
    25741 ctttttgaat gtgaacaagg gttggaatgt tgtgtttaag tggtgtctga ttatctttgt
    25801 tgcctatatg gtagcaggat ttattttcta ctttatcaag caaatcaaga gaaagtcatc
    25861 ctaatactct tcgaaaatct cttcaaacca cgtcagtttc catctgcaac ctcaaaacac
    25921 tgttttgaac aacctgcggc tagcttccta gtttgctctt tgattttcat tgagtataag
    25981 atacaaggga ttcattttcg aatttaagaa ggagtctaca aaccaatgag gcttcttctt
    26041 ttcttgctta tttgataact atcaaaaaag tagaagatta agagaaatat agaaagagat
    26101 ttcatgaaac aatttttaga acgggccagc attttggctc tctccctcgt tttgattacc
    26161 tccttttcca tttcgagtgc cctaccagcc atgtttgact attatcaggg ttattctaag
    26221 gaacaaattg agctcttggt gagcttgcct tcctttggaa tcatgatgat gttactgcta
    26281 aatggtttct tagaaaaaat atttcctgag cgcttacaga ttagtttggg cttgctgatt
    26341 ttatcattga gcggtacagc tcccttctgg taccaagcct atccctttgt ctttggaaca
    26401 cggcttctct ttggtttggg tcttgggatg atcaatgcca aggccatttc tattatcagt
    26461 gaacgctacc aaggaaaaag gcgaattcag atgttagggc tacgcgcttc tgcagaggtc
    26521 gttggagctt ctctcattac cttggccgtc ggtcagttgt tggcctttgg ttggacagct
    26581 atctttctag cctatagtgc tggatttttg gtgctgcccc tttatctgct ctttgtccct
    26641 tatggaaaat caaagaaaga agtcaagaaa agagcgaagg aagcaagtcg tttaactcga
    26701 gaaatgaaag gcttgatttt taccttagct atcgaagcgg cagttgtagt ttgtaccaat
    26761 acagctatta ccatccgtat tccaagtttg atggtggaaa gaggattggg ggatgcccag
    26821 ttatctagtt ttgttcttag tatcatgcag ttgatcggga ttgtggctgg ggtgagtttt
    26881 tctttcttga tttctatctt taaagagaaa ctgctcctct ggtctggtat tacctttggc
    26941 ttggggcaaa tcgtgattgc cttgtcttca tccttgtggg tggtagtagc aggaagtgtt
    27001 ctggctggat ttgcctatag tgtagtcttg acgacggtct ttcaacttgt ctctgaacga
    27061 attccagcta aactcctcaa tcaagcaact tcatttgctg tattaggctg tagtttcgga
    27121 gcctttacga ccccatttgt tctaggtgca attggcttac taactcacaa tgggatgttg
    27181 gtctttagta tcttaggagg ttggttgatt gtaatctcta tctttgtcat gtacctactt
    27241 cagaagagag cttaggattg attcctaagt ttttcttttg atactcaatg aaaatcaaag
    27301 agcatagttg attgagtttg gaatagtacg ctgtagctgc taaaatattt ctagaaatta
    27361 atttgacttt tctaatagag ttattcatat cttatttcaa tctactatag aaagctagcc
    27421 gcaggttgct caaaatagtg ttttgaggtt gcagatggaa actgacgtgg tttgaagaga
    27481 ttttcgaaga gtatgagatt tttataccca tacaatattt gttttaggtc cttgtttaca
    27541 gactaatttc ttgataaaat agaggttatg acgagatata aagcaactat ttcctatgat
    27601 ggttatgcct ttgctggctt tcagcgccag cctcatgcgc gtagcgttca ggaagaaatt
    27661 gaaaaaacct tgaccagatt aaataaaggg caagccatta ctgttcacgg tgctggtagg
    27721 acagatagtg gggttcatgc cctgggacag gtcattcatt ttgacctgcc ttatcagatg
    27781 gatgaggaga aactccgttt tgccttggat acccagtctc ctgaagatat tgatgtgatt
    27841 tcgattgagc ttgtggcaga tgattttcat tgccgttatg ccaagcatag caagacctat
    27901 gagtttactg tggatagagg acgtcccaaa aatccgatgc gccgtcacta tgccacccac
    27961 tttccctacc cactcgatgt ggaacgaatg cagattgcaa tcaaaaagct agagggaacc
    28021 catgatttta ccggttttac agcctctggg actagtgtag aggataaggt tcgcaccatc
    28081 acagaagcta gtttaatagt cgatgagaca ggacaatttt tgacctttac cttttcagga
    28141 aatggtttct tgtataaaca gattcgcaat atggtgggga ctcttctcaa aatcggtaac
    28201 aaccgcatgc cagtagagca gattgatctc atcttggaga agaaggacag gcaacttgca
    28261 ggtcccactg cagcaccaaa tggtttgtat ttaaaggaga ttcgttatga agaataatcg
    28321 tattttagca ctttctggaa atgatatttt tagtggtggt ggactgtcag ctgatttggc
    28381 tacctatacc ttgaacggct tgcatgggtt tgtagcagtg acttgtttga cagccttgac
    28441 agaaaaagga tttgaagtct ttccaactga tgataccatt tttcaacatg aattagatag
    28501 cttgcgtgat gtggaatttg ggggaattaa gattggtctt ctccctactg tcagtgtggc
    28561 tgagaaggcc ttggacttta tcaaacaacg cccaggagta cctgtggtgt tggatcctgt
    28621 cttggtctgc aaggaaacgc atgatgtagc tgtcagtgag ctctgccaag agttgattcg
    28681 ctttttccct tatgtcagtg tgattacgcc taatctccca gaagcagaat tattatccgg
    28741 tcaggaaatt aaaaccttgg aagacatgaa aactgcagcg cagaaattgc atgatttagg
    28801 agcgccagca gtcattatca agggaggcaa tcgtcttagt caggacaagg ctgtggatgt
    28861 cttttatgat ggacagacct ttactatcct agaaaatcca gttatccaag gccaaaatgc
    28921 tggtgcaggt tgtacctttg cctctagcat tgccagtcac ttgattaaag gtgataaact
    28981 tttgccagca gtagaaagct ctaaggcttt cgtttatcgt gctattgcac aagcagatca
    29041 gtatggagta agacaatatg aagcaaacaa aaacaactaa aatcgccctt gtatccctat
    29101 taaccgccct ttctgtggtt ctaggttatt tcttaaaaat cccaacacct acaggaattc
    29161 taactctttt agatgctggt gtcttctttg cggcctttta ctttggtagt cgtgaaggag
    29221 cggtagtcgg aggactagca agtttcttga ttgacctctt atcaggctac cctcagtgga
    29281 tgttctttag cttggtcaac catggcttgc agggattttt cgcaggattt aaaggaaaaa
    29341 gtcagtggtt aggccttatt ttagcaacta ttgccatggt aggaggctac gccttgggtt
    29401 ctactttgat gaatggctgg gcagcagccc tcccagaaat tctaccaaat ttcatgcaaa
    29461 atatggtagg gatgattgta ggatttattc ttagtcaaag tatcaagaag attaagtaat
    29521 actcttcgaa aatctcttca aaccacgtca gcttccatct gcaacctcaa aacactgttt
    29581 tgagcagcct gaggctagct ttctagtttg cactttgatt ttcattgagt ataaagaggt
    29641 tgagtaaaaa gtatttcaaa taagaaaaac gcatagtatc aggtgttgaa tgaactgccc
    29701 cccaaaaagt tagacagaaa aaatctaact ttttggggtg tttttattat gaaattaa
//
LOCUS       DAVBBS010000023        28430 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVBBS010000023 DAVBBS010000000
VERSION     DAVBBS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28430
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.023"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(295..2022)
                     /gene="ezrA"
                     /locus_tag="VQU32_000995"
     CDS             complement(295..2022)
                     /gene="ezrA"
                     /locus_tag="VQU32_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HET2472463.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2104..4050)
                     /gene="gyrB"
                     /locus_tag="VQU32_000996"
     CDS             complement(2104..4050)
                     /gene="gyrB"
                     /locus_tag="VQU32_000996"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET2472464.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFIPDPKIFTETTTFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4065..4628)
                     /locus_tag="VQU32_000997"
     CDS             complement(4065..4628)
                     /locus_tag="VQU32_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2472465.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4713..5267)
                     /locus_tag="VQU32_000998"
     CDS             complement(4713..5267)
                     /locus_tag="VQU32_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET2472466.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5278..6501)
                     /locus_tag="VQU32_000999"
     CDS             complement(5278..6501)
                     /locus_tag="VQU32_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET2472467.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVSLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6538..8988)
                     /locus_tag="VQU32_001000"
     CDS             complement(6538..8988)
                     /locus_tag="VQU32_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HET2472468.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIHISSTKSGRTLLSSL
                     LPESCQAMGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIIGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9187..10116)
                     /locus_tag="VQU32_001001"
     CDS             complement(9187..10116)
                     /locus_tag="VQU32_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2472469.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10256..10543
                     /locus_tag="VQU32_001002"
     CDS             10256..10543
                     /locus_tag="VQU32_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HET2472470.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10577..11911)
                     /gene="ciaH"
                     /locus_tag="VQU32_001003"
     CDS             complement(10577..11911)
                     /gene="ciaH"
                     /locus_tag="VQU32_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HET2472471.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(11901..12575)
                     /gene="ciaR"
                     /locus_tag="VQU32_001004"
     CDS             complement(11901..12575)
                     /gene="ciaR"
                     /locus_tag="VQU32_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HET2472472.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12684..15230)
                     /locus_tag="VQU32_001005"
     CDS             complement(12684..15230)
                     /locus_tag="VQU32_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET2472473.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHIAYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15354..15794)
                     /locus_tag="VQU32_001006"
     CDS             complement(15354..15794)
                     /locus_tag="VQU32_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HET2472474.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15769..16638)
                     /locus_tag="VQU32_001007"
     CDS             complement(15769..16638)
                     /locus_tag="VQU32_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HET2472475.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFTELTNTEANCLVGTKEFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDLESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16680..17408)
                     /locus_tag="VQU32_001008"
     CDS             complement(16680..17408)
                     /locus_tag="VQU32_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2472476.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            complement(17416..17895)
                     /locus_tag="VQU32_001009"
     CDS             complement(17416..17895)
                     /locus_tag="VQU32_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2472477.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVDHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVK"
     gene            complement(17906..18604)
                     /locus_tag="VQU32_001010"
     CDS             complement(17906..18604)
                     /locus_tag="VQU32_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HET2472478.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18597..18863)
                     /locus_tag="VQU32_001011"
     CDS             complement(18597..18863)
                     /locus_tag="VQU32_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HET2472479.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(18929..19855)
                     /locus_tag="VQU32_001012"
     CDS             complement(18929..19855)
                     /locus_tag="VQU32_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HET2472480.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19930..20745)
                     /locus_tag="VQU32_001013"
     CDS             complement(19930..20745)
                     /locus_tag="VQU32_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472481.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20749..21069)
                     /locus_tag="VQU32_001014"
     CDS             complement(20749..21069)
                     /locus_tag="VQU32_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET2472482.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLTKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(21366..21446)
                     /locus_tag="VQU32_001015"
                     /pseudo
     CDS             complement(21366..21446)
                     /locus_tag="VQU32_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(21490..23487)
                     /gene="metG"
                     /locus_tag="VQU32_001016"
     CDS             complement(21490..23487)
                     /gene="metG"
                     /locus_tag="VQU32_001016"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET2472483.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEYDGKLTLLTVDPAVPNGSVIG"
     gene            complement(23611..24870)
                     /locus_tag="VQU32_001017"
     CDS             complement(23611..24870)
                     /locus_tag="VQU32_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2472484.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKKSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(24872..25573)
                     /locus_tag="VQU32_001018"
     CDS             complement(24872..25573)
                     /locus_tag="VQU32_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2472485.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(25557..26756)
                     /locus_tag="VQU32_001019"
     CDS             complement(25557..26756)
                     /locus_tag="VQU32_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET2472486.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTAVKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILIPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            27042..28388
                     /gene="gor"
                     /locus_tag="VQU32_001020"
     CDS             27042..28388
                     /gene="gor"
                     /locus_tag="VQU32_001020"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HET2472487.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
BASE COUNT         8500 a         6262 c         5073 g         8595 t
ORIGIN      
        1 aagttacttc aaagtacaga tttgaagctg cagataagac tgacgaaatc actttaatac
       61 taaatgaaaa tcaaaaagca aactaagaag ctagcctcag gctgtacttg agtacggtaa
      121 ggcgacgctg acgtgatttg aagagatttt cgaagagtat aaaacactgt tttgaagtgg
      181 tggataaatc ggctgggtaa aaactcaatc tcaggagaaa atgaagtaaa tctccccaca
      241 actatagaaa aagatttgat tctgctcctc acacaataaa atctttttct tttattaaaa
      301 acgaatcgtt tcacgtgttt tctcatatga ggtaacaaag cgattggtta caccaggctc
      361 tgccacttcc aatgcttgag aaatcttgtc aaatgaagcg tgataatcaa attctttttc
      421 aaaaatatct aaagcttcgt taaatgcttc ttgaatgcgt tcatcaaatg agcgatagcg
      481 gttagaatat tgcaagagtt gctctgtcaa agttgcatat tgtacaatat tataagtttc
      541 cgtttctaaa gcttccatat cattcgttgc aatttcaaga actcgggtaa cagattcaat
      601 gttaatcatt ttttgttcta actcaaccat taaatcctcg gtattattgc ttgccgtaaa
      661 gaataacttc aagaaagttt gtggaatacc tggcagattg cgtttttcca tgtatcgctt
      721 gatagtatgg agacgattga cataaacatt ggccttttga cgtgcattaa tatcatcttt
      781 ctcaatttgt gtcaggcgct cactaactga aatttgctca tcttcaatat cttttagttg
      841 agtttgtaaa tcctcaagat tttcttcaag aactgaataa gcttgggttg gttcttcttg
      901 atttgaagtt acctcaacaa tagctgcctc aaaactctct aattctgtct gaatacgacg
      961 aacatggctt gcagctgtct caggaagtaa ataggtcttg ttcaaacgtg caatatcttc
     1021 tcccaataaa gtattattct ctttcatatg ttgaaggtaa gttggaagag ttgcaagtag
     1081 attttccact actttctgag cagcaatttc tcgagtaaaa atatcataca aggcattgat
     1141 ttcctcttgt gcctgtccat tctcatattc ggcattatcc aattccaact gacgaatatt
     1201 ctcttggttt ttcttgaatg cttcataaag caagtggaaa cgcgcttcaa tatccgtttc
     1261 aacaaaatga taattagcat caattagttt acgataaccg gcttccaaat cctgtaattg
     1321 atctggcaat tctgtagaaa gcgtcgtaac caaggctgga acacgatcca caatatgact
     1381 taaggccaaa atgtgatttt ctgtattatc caaaatcact gcggcttcca cagggtcacc
     1441 cgatgaattc aaggttacaa attgtgaaaa ttcagattgg atattttcta attgtttttc
     1501 aatttcatcc aaggcttgac catactgttc tgaattttca gcaactctat gctgaagttc
     1561 ctcaaataaa tccaaagcat gaagaacacg accactattt ttagattctt gcttctctaa
     1621 gtctgccaaa gcattgcgaa ttgccgcaat atcttcttca atcaaagtaa tttgactctc
     1681 aatttggtca atttgatgac tggccttgag aaaacgaaat gaatggttat aaccttctgc
     1741 ttcaaagaga ttattttcaa tatcggcaaa agagttgaga gataaatcga cccatttttg
     1801 attccattca cggaaagcca cttgactttg tccaatcaag tgcatatttt ttacagcttc
     1861 tacttcatca tttactggaa gattgtatag ttcttctttt ctttcttcta gcgcctctaa
     1921 tctcccctcg tttcgcttac gtagaaagat tgccactaca taagccagaa ctaaaatgac
     1981 tgcaattgca accattaaat aaattagttg tccattagac atatcaaact ccttttttac
     2041 ttgaaacaat cgtaacgatt atatcatatt ttttagacca agggaactac ttctccctat
     2101 tttttagaca tcaagtgtac tatagacagc attttcttcg ataaactcac gacgaggctc
     2161 tactcgatcc cccatcaaca tatcaaagat tttatctgct tctgcagcat catctacaga
     2221 aactctagcc atcaagcgat gttcgggatc catggttgtt tcccacagct gatgatcgtc
     2281 catttcacct agccccttat aacgctgaat agtcggtttg gtacgacctt cactataacg
     2341 ggctaaagct tcttggagtt tgatttcttg atctgcaccc ggctggatat attctttaat
     2401 ctcgcttcca accttgacac catagattgg tggttgggca atataaacat aaccagcttc
     2461 taggattggt ttcatataac gataaatcaa ggttaaaaga agggtacgaa tatgggctcc
     2521 atcgacatcg gcatcggtca tcaaaacgag tttttggtaa cgggctttcg aaacatcaaa
     2581 ttctgcgcca aatcctgttc ccatggctgt gaaaagacta cgaatttctt cgttagctag
     2641 aatcttatcc atacttgctt tttcaacgtt caaaatctta ccgcgaattg gaaggatagc
     2701 ctgaaactca cggttacgac cagatttggc tgatccacca gctgagtctc cttcgacgat
     2761 gaagagttct gtttcagcag ggttattaga agaacagtct gctagtttcc ctggaaggtt
     2821 ggaaatttcc aaaccagatt ttttacgtgt gacttcacgc gcacgcttgg cagccacacg
     2881 agccttggca gctaaaatcc ctttttccac gatacgtttg gcaatctgtg gattttccat
     2941 gaggaaatcg gagaaggctt cactgaagag gcgattggta atcttgacca cttcgctatt
     3001 tcccaatttg gtcttggttt gtccttcaaa ctgtggattt gggtgtttaa ctgagataac
     3061 tgcagttaat ccttcgcgaa catcttcccc tgttaaatta tcttcattgt ctttcagtaa
     3121 cttattctta cgagcataat cattgataac acgagtcagt gccgtacgga aaccttgttc
     3181 atgcgttcca ccttcatggg tatgaatatt attggcgaaa ctcatgacat tttcatggta
     3241 acctgttgtg tactgcatgg ctacctcaac tgtgatatca tccatctcac cgtctgtata
     3301 gattggtgta tcaaagatta catccttgtt ctcgttgata tattcaacgt aactagcaat
     3361 cccaccttca taatgataat gcttggtttg ttccaaacct tggcgcttat cagtgataga
     3421 aatttgaaga ccgcgattta gaaaggccaa ctcttgaatc cgtttattta atttatcaaa
     3481 atcaaaggtt gttgtttcag taaagatttt tgggtccggt atgaagtgaa ccgttgttcc
     3541 agttctatct gtatccccaa ccacctcaag gtctgcaaca acatgaccac gacggtattc
     3601 ttggtaatga atcttaccgt ttttatggac atgaacgtct aattgagtgg aaagggcatt
     3661 aactactgac gaccccaccc cgtgaagacc acctgaaacc ttgtatccac caccgccgaa
     3721 ctttcctcca gcatgaagga ctgtaaagac ggtctcaaca gcaggacggc ctgttttttc
     3781 ctgaatatcg actgggatac cacgcccatc atccacaaca gtaatcgaat catctggctc
     3841 aataaaaact tgaatatggc tggcaaatcc tgccaaggcc tcgtcaattg agttatcaac
     3901 aatttcccag actagatggt gaagaccttc ttttgaggtt gatccaatgt acatccctgg
     3961 acgcatacga acagcctcta agccctctaa aacttgaatt tgactggcat cataatcctg
     4021 tgcctgcaga tttttgattt cttctgtcat cttattcctt tttcttatat gtctaatact
     4081 tgtcttaaat tcacgatact ggtaaacaag tgggtatcaa gtcctgcagc ttgacctgct
     4141 tcgatatcaa tcggccgatc accaatgaca agaccagagc taatctgata cttttctctt
     4201 aaataaagca tggattcggg atttggcttt ctcttaaagc ctgagctaga agtcaccact
     4261 tctgtaaaat aagctgctat agaggttttt tctaaaattt ccaaaacctg atcatttcga
     4321 tgagagacca aaaaatgacg gccaccttga tttaaaatgt cttccaatag gtcagaaact
     4381 ccttcaaata aaatcgggtg ttcaagctct ctggcttcat tttccttgta cttttctaaa
     4441 aaattctcta aattgggagc gaatgtctca atcgcaaaag gagtagaaac ctttaaagct
     4501 tgatagacac tgtcatggtc ttgtgtgata ccatacagtg ccaatgtttc aacaaatgca
     4561 gctgttgaag tttcataatt atccagtaaa gttccaccta aatcccagat gtaatcgtga
     4621 tatttcatac ccttcattat agcatttttt tatgaaaaaa gcgatgattt ccctgagttt
     4681 tccacataaa aaacgagaga ataactcccg ttttactgat ttttaccaaa gacatctcga
     4741 tagagcattc ctgttcgtaa actctctgcg tcccctccta gttgctccac caactcgtag
     4801 gcaaaggcaa gggctgttga aggaccccga ctggttgtca actgaccatc taccactact
     4861 gtttccttga cgtagtgacc atcaaggatt tgctcttgaa cgccgtcata acaagtgtat
     4921 cgcttatttt tcaatatctc tgcttgattg agggcaattg gtgccgcaca aatggctgct
     4981 agtttcttcc cttcttgctc gaagctttgc aattcttgaa tcaaggtctg attatcacgt
     5041 aaatgtgcag aaccaggcat acctccagga agaacaatca tatcatagtc tgataaatct
     5101 ccatcaaaga catgatctgc tcttacttgg attgcatgcg aacccgttac ttgctcttca
     5161 aaaccaacca tatcacatgt gatattggct cgacgcaaga catctacaac tgtcaaggct
     5221 tcaatttctt caaagccctg agctaacata actgctactt ttaccatgat tttctcctta
     5281 tttaatttgt tttaatacaa cctttttccg tttgaatggg acttttccgc tcttttcttc
     5341 agctagatta gtctggtaac tcatcgataa aagcaaacca acaccaatca gattactgat
     5401 aatagctgat cccccttgcg aaatgaaagg caagggaatc cccgtcaaag gaagtagtcc
     5461 agtcacagca ccgatattct caaagatgtg gaagagcaac atcataatca aacctgtgga
     5521 aatataagtg tagaactggt tatttgattt aagagtaatc ttcaacatac ggtaaatcaa
     5581 catgagatag agggcaataa ccaggacaga gccaataaag ccaaaatctt ctgcaataac
     5641 cgtaaaaatc atatctgact ctcgaactgg gataagcaga ttcgaagcat taaatccctg
     5701 accaaataag ccaccactcc caatggcaat ctgcccttga gcctgctggt aagtcgttgt
     5761 ttgggcaaac tcaaagggat tgagccaagc caaaatccga ttgatttggt aggtcggcat
     5821 tccaatctgg tgaagaaaag ctcgtccgtc cttgctaata aagatagcta agaaaccagc
     5881 aactcctgtt acagcagtca caaatactgg gataataatt ttccaagaaa cccctgataa
     5941 taaaacgatt cctgagaaaa tggctacaaa aaccaaagcc gtccccaagt cactttgaag
     6001 tgctaaaaga actaggactg gaatggtaaa gagaatcatc cagaaaatta acaaaaagtc
     6061 cagcgaaacc gtgcgtctcc attccttatg tttctttgta aattggacaa tgacacgagc
     6121 caacatgagg atataggata tcttcataaa ttctgacggt tgaaataggg taattccatt
     6181 tattgatacc cagtttttgg cacccgttga tgcaactaag cttggattat aaaatacaat
     6241 cggcaagatc ataagtccca agcctaaaat atatagaaag ggggtcacct tccaaagaaa
     6301 ttctgtatta aagagcatga ccacaaaacc aatcacaagc cccaaggcaa tccaggcgac
     6361 ctgctgccct aaaatgggca gaatattatt gggataatca tgactaacgg ctatatagat
     6421 agccaccaca ccgatgacca gtagaaaaaa tactggcaag agcaaactat aatcgactct
     6481 agagtcgaga gaacgtttca tatatactaa ccttatactt tcatacaata ctatttatca
     6541 aagttcatta aaaaatctat caatagcctc gtcaacttcg gatcgagaga tggttttaac
     6601 agtcgcttct tctgctagag atgctactat ttgtttgccg tatcgttttc cgatgattct
     6661 cctatccaaa ataagagtta aggaacgttg gtattcacgt ctcatacttc ttcccaaagc
     6721 ctgttttaaa cgaataatgg ccattggcaa ttgataatca taaaaggcat ttttcccttc
     6781 ttgattcagt tcttgattaa tctttttcgt caagggttct tgaggatttt ggaaaggaag
     6841 cctcggtaca acttgaatca caaaaggatg gcttgaaaaa tcaactccct cccagaaact
     6901 tgctgcacca agcaagattt gttgttcacc tttttcaaag cgtttcttta gctgatgaac
     6961 atccccattt ttatactggg ccaagtggct aactgtaagt aaatccgata ctgctagaag
     7021 catgtcttta gcggtaaaga gaaccaaaat cggttgctgg aaagcttgaa tttccactag
     7081 taaagcagct acctctctgg catagacttc taaggaggtt tctgttacca gagggaaatc
     7141 tttgaccata accacttctt gttcctgttt tccccgagat tcaatcttga caaatttggc
     7201 ttcaggatag cctaaaaggt ctgccaaaga aaccctctga ctaatctcaa gagtagccga
     7261 tactcccatg gcttggcaac tctcaggaag taaagaggac agaagagtac ggcctgattt
     7321 tgtagaagaa atatgaattt tcttttgact cgtttcagtt acttcaagcc agtaatcacc
     7381 ttcagctgta aaatagcgaa ccagctcatc aaagccttct acttctaatt ctgaaaaata
     7441 ctgatggaga ttgtccagag aatctaagat atttttccta gatttaccag actgaaattg
     7501 ttctatcaag tagagacact caaagcgaat actttctagt atccgttgtt gaaccctgtt
     7561 ttcttctcct actaaagcct tatcaattaa atcgataata gattgtatat cgtaggtctc
     7621 ttgaagcaga ttttctagag ctaacaaaat cttttggact tcatcaataa tcagtaaacg
     7681 gtcactgaca aattcaggat tatcttcaag tctggtcacg agataggcat gattagtcac
     7741 taaaagcttg caagtctctg ccctttcttg actacgtttc caaaaatctt ccgtcacaaa
     7801 taagctctgg gatgataaat tcccatcatg acgaaggtct gctagaaaat gttggtaacg
     7861 atagagttgc ccgatttcat ccaaatctcc tgtctctgtc tcagtaagcc agaccaagac
     7921 ttgcatttta aagcgtctaa ataagcgatt ttcatcattt tcctgcaagg aatgataaaa
     7981 ggcatccaac ttcagataat tttgtggtcc ctttaagcta tgaatatctg tatggaacac
     8041 ttccttgagg cgtttacctt cttcttccat gatttgattt tgaagaatct ttgtcggaac
     8101 actaagaaca atttgtcgct ctttggattg agataaagcg ggtaagagat agccataggt
     8161 tttcccaatc cctgtcggcg cttgaatcag agagacaggt tcatctttca atagcaagcc
     8221 aacctcttta gcaaaacttt cttgttcctc cctcacttca aggttcaaca gagaaatatt
     8281 tttagaaaag tcttgagata gttttcgtgg ctccagagaa gctgccgttt tcttaaaata
     8341 cagaccttga acttggacca agtctggaga actcaggata gattggttgc gataagtttc
     8401 ctcaataacc aggtaggact catataggag agcgtcagcc atttccagca agcgttccaa
     8461 gagaccttta ggaagctgag tcatcttttc ccgtaaaaaa agaagtaatt ctgctgtagc
     8521 ttgggcatct gaaagggctg tgtgtgcgtg tttaagagga attcctaatt ctcgacacaa
     8581 aatcggcaag ctatattttt ccagttcagg gaaaaagacc tgggccaatt cgaccgtatc
     8641 aacacgaggg tttcttagct catagccttc aaaaaataaa ttttccgcca agagattagc
     8701 atcaaactga acattatggg ctacaaaaat cccatcctcc accaagtcaa atatttttct
     8761 ggcaacttgc gaaaaatcag gtgcttgcgc cagacgttgg tctgtcaatc ctgtcagttc
     8821 tttgatatga gcatccaagg gttcatgtgg attgacatcc gtcgtatagt gatcgacgat
     8881 ttctccgtcc tcaatcacga caattcccac ttggataatt ttagccttac tacctgtgct
     8941 agttgcctct aaatccacca caacatagcg attaccaatc gttttcatta taaaaaatta
     9001 taccaaaaaa agggccattt ggcacctgca aacataggat aattttcaaa ctcaagaaca
     9061 ctacttttct taaaaatccc atcgaataga gcattctagc ctgctgattt tttaaaatag
     9121 tgaagaaata cgaaaaattg agagaagaaa tgttttcatc ttccttctct caacgaatca
     9181 tgtattttat ttaaccatgt caggatcgta gtcataaaat cctgtatcaa ggaaacggtt
     9241 tttagggtga agtttacgaa cttcttcatc cagcaagaag gcttggtcat ggctaaagtt
     9301 gccctcttga atcaagctac gcgcttggat aaagagtttt gcaatctcaa caaagttctg
     9361 accaggagtg tagagccctt ctagtttcca gcgagtaaag ccatgctcca ccaattctgt
     9421 taatttgatc atcaaatcaa ggtcattgtt ggcaaagata tgggtcccat gattatcttc
     9481 aaaaatggaa tagtggctct ctggatcact tggctcagcc aagaagaggt cacgtttatg
     9541 cgtcttttca tcatcgatat gtgtaaagtt atagtagttt tgcaagagtg gacgtttaga
     9601 atgatggatg acgctagcac cgtaaaccaa aacttcagca ggaatttcca aaatctctgg
     9661 cattttgaaa agttcagctg atggaatttc acgcgccaaa acagcctcag atgcgccagc
     9721 cttttgtccc cagaagttaa tctgacgact gctagttacc atggttgaag catcgtagat
     9781 ggtcttaaat gaataaccat cgcggttaac tacgtaaaag acgcctgcat ccccaatcgt
     9841 aatatagtct gtcttgattt cttccaagaa gtctaagaaa ggcttgatac ggtccatcat
     9901 atcttggtgc atgagagcat tgaccgcaac gagcaattcc ttaccagcat catgaaccaa
     9961 cttagcgatt tcacgtaatt ggtcataact aaaggtcgtt ggcagacgaa ggccaaaatc
    10021 tttctcaccg acatagatac ggtctacgcc agcttcgagt agttgttcaa cttgttcaat
    10081 actttcagca gttgctgtaa tgacaatctt ttccataaga aaaattatac cacatttctc
    10141 caaaatcatc catgctttga aaatgaaaag ggactttatt ctttttgtaa cctttctgta
    10201 ataattctct gccgaaaaaa tgataaaata ggtatgtact gttaaggaga gaattatgcc
    10261 cgtaagaaaa ttacaatcct atgaggtaga ctatcaagaa gaattaaacc agcagcttcc
    10321 tcattaccaa gcttatacac ctgaagcgca atctgatgcc aatctcaagg aaatcctatt
    10381 ttttattaat atcgctgttt tttgtatctg tattgctatc tttagtttta tctttttagc
    10441 attaaaatta tcaactgctc ttgcctttgc cgcagcaatc ggattcagct tacttgtttt
    10501 aaaagttcaa cggtctatta tcaaacgaaa acgtagaaga taaatccaaa atatcgcccc
    10561 agttggagcg atatttttat tttttctttt tagatggtgt ctgaatggca atcttcactt
    10621 caaagattgt tcccttgggt ttattatctt tgacagtaac agttcctttt agagcatcta
    10681 caatttgctt ggctagggat aatcctaaac caaaaccacc tttttgccga gttctagcct
    10741 tgtctactcg ataaaaacgg tcaaaaattt tctttttatc ttctgtcgaa ataccgattc
    10801 cattatcaga aacaagtaaa taaagattgc gatcggtcgc cgagataaga aaatcaattt
    10861 caccatcctc ctcagtatac ttgacggcat tatcgaaaag aatggtcatc agttgtttca
    10921 gaagaagctg atctgtgaca attgttcgat ggatacgatt ttcaaaacgg aagacacgat
    10981 tattttccga agcaatcatc tcgtagtttg tgaaagttgt attaaaaaag ctagttggaa
    11041 cttctgcaag ctccggctta atcccatcat ctctccgagc taagttcagc aagcttgtcg
    11101 ttaaaaaacg catatttcgg acttcttcca aactcgatgc aatgctttcg ctcacatcca
    11161 taatggtagc ttctggctta cgaaaaaggg tctctaagcg attttgcaaa actgcgagtg
    11221 gagttcgtaa ctcatgactg gcattttcca caaaagactg ttgcttctgc atactctcaa
    11281 gcaggggcct aacactgacc ctagctagat agagactggc aagtaaagac aaaatccaga
    11341 aactagccat cacgaccaca atcaattgct catgcttttg actagcctgt tccaactgac
    11401 tggtattaat caagacagca gcatacttga tattggttga aaccgaacta atattggtct
    11461 ccatcaaaat cacacgatag atttcttcct gcccatagct attaaaaacc tgaatctggt
    11521 agatatgtcc tagttctttc ttttctaact taatcttatc caagcccaaa aatcgatttc
    11581 cagaaagaag ttgagtaaag tctttatcaa agagaatgac ttccgtattg gaactgacat
    11641 taggttttat ttctacttta ctagcgtccg ccctagcatt ttctaaatct ttaatctctt
    11701 ctgttgccct atttatagcc agctgaataa ctgcttgagg attttcactc agtccatgaa
    11761 gcttatcgtc caccgaagta taaagactcg aatgcatgac ttgtaaaata atcagagtca
    11821 ttgtagaaaa aatcagggtg aagacaccga agttgcggat aaaataacta aagtcatccg
    11881 cataccatgt ttttttaagt ttactgaaca tcttttaaaa gatacccaac actacgcaaa
    11941 gtttgcaaat tctctgcaaa agtggttccc tttaatttct tacggacttt tgaaacatag
    12001 acttcgacaa ccgaaatcgt tgtatcacta tcaaatcccc atagacggtc aaaaatctgc
    12061 gtcttaggca aaatcacatt ttgattttga aggaaataaa ctagtaaatc gaactctttc
    12121 cccagcaatt cgacaggagt atcttcaact ttaacggtat tggttgataa attaaccacg
    12181 atattcccat aagtcaaggt gttttcatta aacttccctg aacgtttgag aagggcctga
    12241 atccgcattt taagttcttc taggtagaaa ggtttggtca gataatcatc cgctcccagt
    12301 tcaaatccat gtcccttgtc atccaaactt tccttggcag tcataatcag aactggtgtc
    12361 gtaattccct tttcacgcaa ttcttttaag acttggaaac catttttttc tggcaacatc
    12421 aaatccagca aaatcaagtc atagacacca ctctcagctt cgtagagacc ttcttctcca
    12481 tcaaatacct gcataacatc cgcaaaatcg tctaaaaagt caaatactga atttgacaga
    12541 cctaggtcat cctcaaccaa taagattttt atcatgagaa actcctcctt attaaaacta
    12601 ttataccaaa tttgccttaa aaaaaactca actctctgca ttttacatga gatagctgag
    12661 ttttcttttt attttaggct tatttatgca tttccgtatt gaagaacaac tgcttcgact
    12721 gcagcttttt cacggctaat caagtcaaca cgcgctgcaa tttccttgat tcccataccg
    12781 atgttacggc taagagcaag gtcagaaagt tgcggctcaa agaactcctt gtattccgcc
    12841 aagcgttgct gagtcttaaa tacatgagca ggaaggataa caaagctatc aaagctcata
    12901 tctcctccaa gggctgcctt aatccaagcc cagttttcac gcgcccaaga ccaagctgtt
    12961 ttctgagttg cttgatgagc taggaattgg taataccaag cagacaagtc ctgtggtttg
    13021 accacaaatt tgtccttcca agaagtaatc aagttttgga tattatccgc atctgtactg
    13081 tatgcaagag ctgctgccaa ctggcgttta aagacagcat ctgttgcgtg agtataagta
    13141 tcaagataaa gtgctaacaa gtctttagtc tcatgatgtt tcatctcatt aatcagaact
    13201 tgtgaacgaa tagctgctgg gagtcctgca agattctcct tgtgtgttgc gaagatttgg
    13261 ctagcgactt gactagcttc tgcatcattt gagcgaatca tcatcgaaac agccagctga
    13321 cgaaccaatt catcctcatc tgattctccg tctttagctt caaaaccaag acggtcatag
    13381 ttatgacgag ccaatttagc aaccagtcct ttgaaggctg tttcagcatc cgttccttca
    13441 tcaataaagc gctcaagggc tgaaatcact tgagaaacag ctgaaaccac cagataagac
    13501 tcttccttag caagtttatc aagaactgga agcaagtctg cataagcaat gtgccctgcc
    13561 tcagccaaca aacgacgttc ttgaacaatt tgcagtttgc ttgtgttatc aagtgtctct
    13621 agctcagcaa gaacagcttc taacaagtct ccttgatagt cggtaatata gtgggcagta
    13681 ttttcagtgt tgagacgaag agctccttca ttttcagcaa gaagagctgc gtagccaggg
    13741 atttcgatac tttcagtttc gagtgtatca ggcaagcctt tccagttgct attgaggggc
    13801 accacccaga gacggttctt gtcttcgttc tcaccgatga agaattgttt ttgtgaaatc
    13861 ttcaagacat cattttcaac tttaacagta agaactgggt aaccaggctg ttccaaccaa
    13921 gaatccatga aggctgcgac atcacgtcct gacgcttgac caagggcatc ccaaaggtca
    13981 ctaccaatgg tgttgctgta ttggtgtttt tcaaagtagg cgtgcaaacc tttagcaaaa
    14041 tcagcatctc ctagccaacg gcgaagcatg tgcatgagac ggcttccttt ggcatagacg
    14101 atagcgccgt caaagagtgt attgatttca tctggatgtt taacttcgac gtggacagac
    14161 tgaacgccat cagtagcgtc acgttcaaga gcaagaggta ctccacctgt ttggaaatct
    14221 tcaaagatat tccagcttgg ttcgatggta tccacacaga cgtattccat catattagcg
    14281 aaactttcat tgagccaaag gtcatcccac catttcatag tcacgaggtt cccaaaccat
    14341 tggtgagcca attcatgggc cacaacaagg gcaacttgtt gacggctagc aaatgtagag
    14401 ttctcatcga caaccaagta aacttcacgg taggtcacaa gaccccagtt ttccatagca
    14461 ccagctgaga agtcaggaag ggcgatgtgg agagattgag gaattgggta cttaactcca
    14521 tagtaatctt cgtaaaactc gatagagcga acagcgatat ccagtgagaa atcaagattt
    14581 gaaagtggat gtgctttggt tgagtagaca cctaccaggg taccattttt agttttagcg
    14641 gtcacccctt gcaaatcacc agcaacaaag gccaacaagt aagaagacat gcgaggtgtt
    14701 gtctcaaact tccagatacc tgtttcctta cggttttcaa catcgatttc tggcatgttt
    14761 gacaaggcca attcaccttc tgcttggtca aagcgaagag agaggtcaaa agttgctttg
    14821 gcttcaggct catccacaca tgggaaagct tcgcgcgcaa aatggctctc gaactgagta
    14881 gacaagacct ccttcttgac tccatcaact gtataataag aagggtaaat ccctgtcatg
    14941 ttgtctgtaa ttttaccaga aaaggcaaga accaattcaa cttgaccagc ctcagccaat
    15001 tcgatatgaa gggcttcatt gtcatggtca actgtaaatg gacgagcttg acctgcaact
    15061 tctacagagg tgatttccaa gtctttttgg tggagggaga tgcggtcact ctgtgcttga
    15121 ccagtgatgg tcactttccc agaaaaagtc ttggtctcac gactcaaatc taaaaataaa
    15181 tcataatgtt caggaacaaa ttgcttaata aaatgttcaa ctgcttgcat agttttctcc
    15241 tattctaagt ttaagagcta ctgctcttct tcaaacaaac ttattatatc atattttgct
    15301 tgagaaaaaa ggattggaca gcgatttcca aaactttctt ccttctaaat agtctacagt
    15361 gggtagacct tgcgaaattc ttctaaaaca accttgcttt caggtgtaaa agtcagccct
    15421 acttcaccca aacagtctgt gaaaaagtcc tcatgaacct ggcgccaata ggcaagtgat
    15481 ttgtctccct caccttcctt aaaggcatgt tgcgcagaaa cttgattgaa gagttcaaca
    15541 gaaacctttg taatttcgac aatgcagaca gactgatttt gactatctaa aatgacatcg
    15601 aaggtcccta cttggggaag tggttcgtct tctagtacat agaggtcata ggctgatgct
    15661 gttgctgtct tttctccttt aaacaccaaa tccgctaaaa ggtctggttc aactccaaaa
    15721 gcccaagcat cgatttcatc tccgatcaaa ggattgattt gcttgtattt attccacatt
    15781 tcttgcggta tcatgggtac tcctttgtaa ttttttactt tcttctttta tgtgtttaag
    15841 atgatctgga tggtcaatct ctaaatcaaa aatctctgga atagaactgt agtggataat
    15901 gcacttgata cccaactgat tcattttttg tatgaaagaa gtattcagat agcctgctac
    15961 agcaaaatta atcttgttct ttcttgcttt atcctgcata tctcttagca tatctaacat
    16021 tattggactt tccagatcat gccattgact gtttctcata gtcgcaaaaa caaaggaagt
    16081 caaatcattc attccaacta caatctttga aatgcccgtt tccagtatac tagataagtc
    16141 aaaatacgct gacggtaatt caatcatcgt tccgactttc ccagtaaagc catactgacg
    16201 caatactgta atagcttgtt ttaattggtc ggcatcattg acaaaaggaa agataacaga
    16261 tagattgggg ttggtttgat aaacttctgt aacgacatgt gcttcagcct gaaattcatc
    16321 caaacacgcc agtaaacgcc tggttcctct atagccaaac aagggatgcc cttcgtcaaa
    16381 aaactcttta gtccccacta aacaattggc ttctgtattc gttaattcag taaaacgata
    16441 ccaaacttct tcatctgagt acagggagca gatagtctct agataatctt ttacaaattg
    16501 ctgacaactt gctaatatga tgttttgatt aagctctctc aacaaatatt ccccacgaat
    16561 catgccgacg tggtgaaata gttgaggata aattttttca agaatttttt cgccactaag
    16621 ggcaagttga tttctcatag ccattcacct cgtcttaaaa gatacaatat catatataat
    16681 tatatcaaaa tttcctctag ttcaaaagct tcataaggtt cagataactc atatcctaaa
    16741 aattctgcta cttttttgga agcctcatta tgagcatccc aaagtggaaa catatctcta
    16801 tttaaactct ctagaatcat tgcagcacca agctttttag caaatccatt tccttgttcg
    16861 tttggtctag ttgcaacttc cacttcaact gctttacggt aaactaaccc tgaggaaatc
    16921 ccagcaatca gttcattatt tttaagaatc acaaagccaa atccaccttt taaaacaaaa
    16981 tcctgatagg actcaaaatc cccctgtaaa tcctgtgacc attcttccgt agaaaagcag
    17041 ttataaatat gattatcaat aggcacaata ttgtaacctt cctctaaatg agtaactaga
    17101 ttatttaaaa attcaacttg aaaatttgcc ttatctttaa aagaatagcg agtaaaagaa
    17161 tttagcccca cttttcgatg cagaaaatct tgccaattag tattttctga aataataatc
    17221 ttataatcta aaccatattt tctaacaaaa tcttcccaaa atctggaatc caactcccct
    17281 gctaggtata gaaaattccc aacatcataa aagcttgttg tcctatcatt gtttcggtag
    17341 acagttccaa tttgagattt aagaccatag agaaccatat ttttcttcca tgttttaaat
    17401 agttccatta tccctctact tcaccttcca attcatgtaa gaagtcttgt ccagttctgg
    17461 aaatcctaat aattcagact taaccttcaa gactaatggc gatgcatttt cttctgtaat
    17521 ctcttgaata tccatccaaa tatatccaag tgaatcattc gcaccatcag acacagcttc
    17581 cgaagtcgta acttgaggtg cactctcatt catttcaaca tcatacaagg ccatgacatg
    17641 gtcaaccata aaatttgtta actcttctct gacgaaaaca tcgtagattc gaggattaga
    17701 gtagcttcta acagtaaatc ccgtctcttc cataacttct ctagtcagcg tttccgtcag
    17761 tccttcacca agttgctgac tgcctccagg tagatcatac cgatgttgat aagggcctct
    17821 cgttttttca atgcagagta acttcccatt ttctaagcaa acagcgtaga ctccaaagtg
    17881 ttttttgatt tccatccaac tcctcctact tcaaagacca gccaccatct attgtcaaga
    17941 tttgtccttg catggcgctc gcttttccac ttgctaaaaa aagactaagc tctgctattt
    18001 cctctggctc aatccagcgc ttgattggag tttcactagc cacccagtca gccaaaccac
    18061 ctggttcaaa atccgcagcg gtcatagctg tcttgactgc tcctggagcg ataccaaaga
    18121 cctgaatccc agcttcagca tagtctagag ccaactgctt ggtgaagcca gccaaggcat
    18181 gcttggatga agtataggcg tgaccacctc cacctgctag gctagaagca atggaacaca
    18241 tattgatgat gattcccttt ttattttcca gcatttgtgt caaataatac cgagtcaact
    18301 ctactggaat aatgtagttg atttcaaaaa tctcttgaat gtcctgcgcc gtttgttcca
    18361 acagtggttt gtaatcatcc aaaactccag cagtattaca caaaacatcc acctgagggc
    18421 accagtcaaa aataggttcc aagtccaagg tcaaatctct ctgtaaaaag tgaaaatcac
    18481 cctctaagag tggcttttca ccttggtcaa ctccataaac ttgatagccc ttctctaaaa
    18541 agaggcgagc ttgagccaat ccgatccctg aactcactcc tgtaatgagt acacgtttag
    18601 tcatgcactt ctacccaatc cgttgccaaa acatcacaaa ctgtcgggct ccacatggaa
    18661 aaaccttctc cttcgccaga aacgttgatt aggaaataag gtgtcatttc aagtgcaagc
    18721 ccattttgct cgatggtatc aaagagttgg acatagtttt ccgcacctcc ccaaccagtt
    18781 cgtacatatt ttctcttagc ctttaaccca ggcaggatct cttcaaatgt catgtttttc
    18841 tcctttaatt ctacattctt catttaatta tagcaaaaaa ccgctttata cggctttttg
    18901 aatgtgagtt attcaaacct gctactactt acggcaaatt attccctgca gcaagataaa
    18961 tttcatacca ttcttttctt gttaagctaa agtttgccgc tcggctaact tctctcaagt
    19021 gcttaggatt tgttgtacct acgactgcct gcatttttgc tggataacgc aatatccaag
    19081 aaatggcaat agttgaagag gttactccat atttaatagc taaacgatca agtacttgat
    19141 ttaaagcttg aaatttctca tttccaacaa aattcccttt aaaatacccg aattgtaaga
    19201 cagaccatgc ttgaatgacc acatcgtgta attggcaata ttcaaaaatg ctgccatctc
    19261 gcatagctgc ttgactatct tccatattaa catgaaaagc tgattcaaat cctggagtaa
    19321 aagccgcact caattgtagc tgattaacag ctaacggctg cttgacatct tttttaagca
    19381 actccatcat cataggattt tgattagaaa ctccaaaatc tcgaacttta ccttgtttat
    19441 aaaggagatt aaaggcttct gctacttggt cagattccat caaagcatct ggtcgatgaa
    19501 ggagcaagct atctagatga tcaatcttca atctttgcaa aataccgtct actgatttta
    19561 taatatagtc cttagaaaaa tcaaaatagg taaattcttc aatgcgaatg ccacatttgg
    19621 actgaatcca catcttttct cttaaatctg gacgattttt taggacaaga cctaacagtt
    19681 cttcacaacg accacgacca taaatatcag ccaagtcgaa ggcattgatt ccaacagaaa
    19741 gtgctgtttc tacaagctct tcaacttctt ttacagactt atcttttatt ctcatcattc
    19801 cgagaacaat ttctgataat tctttgtcat cttgaccaag agttatgtat ctcatcaaat
    19861 ttttctcctt taatttctaa cattcttccc ttcattataa caaaaaaccg ctttgcaacg
    19921 gctttttgac tatacttcac tccattttat cttcttaaac ccacggaaca agacaaagat
    19981 tccaataaag aggacagcta aaggaataac ttttgtaagg aaaacatttg aaattcccat
    20041 ccactcataa taacggagca gagaacccac cacaagatgg gcaataatca tactgacaaa
    20101 tggacgaaag accgcttctt tccaattcca aataccgata actagcgaaa tcgtaaagac
    20161 agacaaacta tcccagggag ccggaatata aaaggctcct tcttgtatga agcttgccat
    20221 tcctacatat cctaaaacaa ctagaagaac tatagtccca acaacaatgt aagtgccaat
    20281 tttcatttta ggagaatctt ggactaaact tcttcgtaaa attgtggcca caagtccaaa
    20341 tccaatcaga aaaataagaa gttgccctaa aaatgtgagc aaattgactg ttaagagagg
    20401 acctttagaa aaatcactta gtagttgata ataacgtaat acagccagga caagaattgg
    20461 cgtcaaaagg gactctttga tagaactgcg aggtgctccc ttgagaatct ctttcattat
    20521 ttttttagga ttcttaccta gataatcctc tgcactcatg ccatctcgtt ctgcttctga
    20581 gaaatctagc atcatcaaat agatctgctc tctgagatag tcttcatcat agagaaatcc
    20641 agcaagatta aaactttccc acaactcctc aaaatacttt tgattctcct cagaaaactc
    20701 atgtagcaaa gcgcttgttt cttcgtaata cttcattttc ttcatggttt aacccccatt
    20761 cttaatccct tctacttttt gactcaaatc gtcccattgc tgccaaaaga ctgagacacg
    20821 ctcttctcct tctttcgtta atgaaaaata cttccgatct ggaccatctg gcgacgggcg
    20881 catgtcgcct cttatccatt gatttttttc taacttttgc aacaaaggat aaatagttcc
    20941 tggaacgata gtatcaaatc cagcctctcg caaagtctga accaactcat aaccataccg
    21001 ctctttttga ccaatcatat ccaagacaca accttcaaga acacctttta atagctgagt
    21061 ttctttcatc acttctccct tctaatctat tttgtaatac ctactagtaa cttcacctat
    21121 agtatattac ttctacacta gtttgtaaag cataatagtt aatactcttc gaaaatctct
    21181 tcaaaccacg tcagcgtcgc cctaccgtat gtatggttac tgacttcgtc agtttcatct
    21241 acaacctcaa aacagtgttt tgagctgact tcgtcagttt catctacaac ctcaaaacag
    21301 tgttttgagc tgacttcgtc agtttcatct acaacctcaa aaacatgttt tgagctgact
    21361 tcgtcagttc tatctacaac ctcaaaacag tgttttgagc aacctgcggc tagcttccta
    21421 gtttgctctt tgattttcat tgagtataaa taaaaaaaca gaactagtct gaactagtcc
    21481 tgtctacttt tacccaatca cacttccatt tggtacagct ggatcaactg tgagaagggt
    21541 taatttgcca tcatattcag ctgatagaat cataccctgg ctgacatatt ttttcatcat
    21601 tttacgtggt ttgaggttag caacgatttg aactttcttg ccgaccaatt cttgttcatt
    21661 tggatagtat tttgcaattc ctgaaagaat ctgacgatct tctccatcac cagcatccaa
    21721 gcggaattga agcaacttat ctgaaccttc tactttagac acttctttga cttctgcgac
    21781 acggatttca accttgtcaa agtcttcaaa cttgatttca tccttgttta gtttgagctc
    21841 aacttcgtcc ggattccatt ctttttcgac tgctggttta ttgccttcca tttgttcctt
    21901 gatataggcg atttcttctt ccatatttag acgtggaaag ataggtgttc ctttggcaac
    21961 tacagtcaca tctgctggga agtcagccaa actcaagttt tcaagactag aaacttcttc
    22021 caaaccaagt tgagtcaaaa ctgcacgact agtttccatc ataaatggtt caatcaagtg
    22081 agcaactaca cgaatgctgg ctgccaagtg gctcatgaca cttgccaatt ggtcacgaag
    22141 agcttcatcc ttggccaaga cccatggtgc ggtctcatcg atgtatttat tggtacgaga
    22201 gatcagagtc cagactgctt caagcgcacg tggatagtca actgcttcca tgtgtgtatg
    22261 gaagtctgcg attgattgtt ctgcaacctc agcaagaaca tgatcaaatt cagtcacacc
    22321 ttctacatag gcagggattt gtccatcaaa gtacttatta atcatggaaa ccgtacggtt
    22381 aaggaggttc ccaaggtcat tagccaattc atagttgata cggccgacat agtcttcagg
    22441 agtaaaggtt ccgtctgaac caactggaag gttacgcatg aggtagtaac gaagtggatc
    22501 tagtccataa cgctctacca acatttcagg gtaaacgaca ttcccttttg acttagacat
    22561 ttttccgtct ttcatgacaa accaaccatg ggcaatcaaa cgatcaggta atttaacatc
    22621 caacatcata agaaggattg gccagtagat agagtggaag cgaaggatat cttttcctac
    22681 catatggaag actgttccat tccagaactt gtcaaagtta ccatgttcgt cttgagcgta
    22741 gccaagagct gtcgcatagt taagaagggc atcaatccaa acgtagacaa cgtgttttgg
    22801 atttgatggg acaggcactc cccatgtaaa ggttgtacga gataccgcca aatcttccaa
    22861 gcctggctcg atgaagttgc gtagcatttc attaaggcga ccatctggcg tgataaattc
    22921 aggatgagct ttgaaaaatt cgaccaaacg gtcttggtat ttgctaaggc gaaggaagta
    22981 tgattcttca gaaacccatt caacctcatg acctgatgga gcaataccac cagtcacatt
    23041 tccagcttca tcacggaaaa cttctgccag ctggctttct gtaaagaatt cttcgtctga
    23101 tactgaatac caaccagagt attcacccaa gtagatatca tcttgagcaa gtaagcgttc
    23161 aaagacctgt gcgacaactt tttcatggta gtcatcggtt gtacggataa atttatcgta
    23221 tgagatatct agtaattgcc agagttcttt aactccaacc gccattccat caacataggc
    23281 ttgaggtgta ataccagctt cttccgcttt ctgctggatt ttctgaccat gttcatcaag
    23341 acctgtcaga taaaatacat cgtagcccat caggcgtttg taacgtgcta ggacatcaca
    23401 tgcgatagtt gtgtaggcag aaccgatatg aagtttccca gatggatagt aaatcggcgt
    23461 tgtaatataa aaattttttt cagacataat ttttcctttc caggcaaatg aaacctgttt
    23521 ttctaacact tcattatatc acatttttaa tgaatttcga tagggaaatc catacaaaaa
    23581 caagatagac gagtgtccat cttgttgatc tcattcataa cgaagggctt caattggatc
    23641 aagtttcgat gccttgttgg ctggcaagac tccaaaaatc ataccaacac tagccgaaac
    23701 tgcaagacta aatagggcaa ctgggattga tactccaact tctatacctt ctattaaacc
    23761 ttgcagtaac aaacctgcta aggcagttaa accacttgca attgtcaagc caattaagcc
    23821 acctaacaag gtcaaaatca tggattcaat caaaaactga attaaaatat tggcacgtgt
    23881 tgcacccaaa gctttacgaa gaccaatctc acgagtgcgc tctgtcaccg aaaccagcat
    23941 gatgttcatg acaccagttc ctccaacaaa gagagaaatc cctgcgatgg aactaataat
    24001 cgtcgtcata aaactaaacg attgttgaat ttctgcaaat acaacggact catctgccac
    24061 ctggtattct ccctgttgta agcctgcaag ctctgtcatt tttcgtgcca gttctggacc
    24121 cagagttggg gttaaactgg tatcattcac tcgaaagaca atactagcta tttcatctat
    24181 attaaaattc gcagcaaggg agatattggt agtaataggc aagccaccaa acccatatat
    24241 ttttgacttt ttagcctccg gactagtata aaccccaatg acccggtaac taaatccatt
    24301 gacttctaca accttgttaa tagcctcttg aggagattca aataaactaa tggacaattc
    24361 ctcatctagc aaaataacac ttgcaaactc tttgaaatct tgctctctca gactacgacc
    24421 tgcaataatt tcattcttaa cagcgtccat gtaagttctg tttccacctg tcaaattagc
    24481 attctcaacc tttttatctt gataggtcaa gatggcattc gttgaattgg ttacatagta
    24541 actatccact cccttcagtt tagctgcctc ttgaacccag gattcttgcg gttttggcgg
    24601 ttcaacagga acttcctctt cctttccaga aaccgtaaaa gctgattgtt tctgagtaaa
    24661 agacccatct ttactttttt taggagagaa aaagacgcta atatttttct gagatttagt
    24721 catatcttta ttgacttgac gagataggga atcacccaaa gccataatca caacaactga
    24781 tgaaacaccg ataataatcc caatcatagt aagcaaagaa cgcatcttgt gagccatgat
    24841 agatgaaaag gcaaatttca gattctgcat cttagttttc ctcctttcct aactgagcac
    24901 tgtcagacga aatgacccca tcccgaatga caatctgacg tttggcatag gcagcaatct
    24961 caggctcatg cgttaccatg ataatggttt ttccttcttt attcaaatca accaataatt
    25021 gcataatttg gttacctgtt ttggtatcca aggctcctgt cggttcatcc gctaggataa
    25081 tagaaggatt gtttaccaag gcacgcgcaa tggctacacg ttgcttttga ccaccagata
    25141 attctgaagg taaatggtga ctacgttctg tcaattcaac cttgtctaaa tattcctcag
    25201 ccaacttgcg acgttttgaa gacgaaactc ctgcgtaaat caagggcaat tctacatttt
    25261 gcagagcatt gagcttcgat agaagaaaga actgctgaaa gacaaaaccg atttgttggt
    25321 tacggacctt agctagttgt ttttcaccaa gcccagccac ttcttgacct tcaagataat
    25381 attctccact ggttggtgta tccaacatgc caatcgtatt catcagagtg gacttaccag
    25441 acccagatgg tcccatgatg gctacaaatt caccctcatt cacttctaga ttgatatttt
    25501 tgagaacctg cagttcttgg tcaccattac ggtaacttct gaagatattt tttagactaa
    25561 ttagttgctt catcagcctt cacctctttt ccttcttcca aggaagatgt tggattactg
    25621 atgaccttag caccgttcgt taaaccagaa gtgatttctt gattttctgc gtcagcattt
    25681 cccaatgaaa cctcaacttt tttagccttt tgttgttcat ccacaatcca gacataattt
    25741 ttactatcat ccattactag actgctaaca ggaataagaa tagccttagt tttgctttta
    25801 acctcaatgt tgacagaaaa accttgtttc aaatcaccaa cctcgcctgt cacatcaata
    25861 gtataagggt atttagaacc tgtattattc ccggctgctg gactagctgc ttcaccattg
    25921 tttttaggat agtcagaaat atagcttaat ttcccagtcc attttttatc aggatacact
    25981 ttagaagtaa agcttacttc ttgacctaca gaaaggttgg ctagattgta ctcagacaat
    26041 tctcccttga cttgtaaatt ttcattgctg acaatatgaa ccataacttg actcgcccct
    26101 gttggagatt tagaaacatt gctattgact tcgactacag ttccctctag ggtactgaga
    26161 acagttgttg catccaattg actttgagcc ttgcttaatt gcgctgcagc atctgcacgc
    26221 gcatcacggg catcacccaa ttgagcatca atagaagcaa cagaatttcc agccactgga
    26281 gttgggcttt gcaccgttgc atcttctcct cctactggcg ctggtaactg tggagcctga
    26341 gctgaagcgg cttcatttcg tgcttgattg agttcattga tatgacgatc tgccttagct
    26401 actgctcgac tagctgaatc ataggccgcc tgcgcttctg aactactgta cttgactaaa
    26461 gcctgccctt cgctgacctt atcgcccaca gaaacaagga tttcatctaa atcaccctta
    26521 ctagcatcaa aataaacata ttgttcattt tttgctgtta ctgtccctga caataaaaca
    26581 gaggatgcca cgcttccttc cttggcaaca acaagatgag taggctcatc ttttacagca
    26641 gtctgagaag gttgtctaaa aagtaaaatc cccccggcac ccaatacaac tacactcgca
    26701 gcaccgattg ctgcatacag ttgccacttt ttagctttac cattcttttt cttcataatg
    26761 aaactccttt tcttttttac aatactttgc tattatacca aatttccctc cagcaaacaa
    26821 tgcagttcag atggaaataa tggcagttca ggattaaaca atcgttcgga attttgcttt
    26881 tctattgtac tagatatata atacactatc ttgacttatt tggcaagctt ttttgcaatt
    26941 ttttgtgcgt agcagatttt tgcaatcaaa tcaaaaaaag aataaaatat aactatcaaa
    27001 aaagacactc tactatttaa aagattacaa aggagttttc aatgagagaa tatgatatca
    27061 ttgctatcgg tggaggtagc ggaggaatcg ctaccatgaa ccgtgctggt gaacatggag
    27121 ccaaagcagc cgttattgag gaaaagaaat taggtggaac ctgtgtcaac gtcggttgtg
    27181 ttcctaaaaa aatcatgtgg tacggggcgc aaatcgctga gactttccat caatttggag
    27241 aagactacgg ctttaagact actgatctta actttgactt tgcaacccta cgtcgcaatc
    27301 gtgaagccta cattgatcgc gctcgttctt cttatgatgg tagttttaaa cgcaacggtg
    27361 tagacttgat tgaaggtcat gctgaatttg tagattctca tactgtaagc gtaaatggtg
    27421 aactgattcg tgctaaacat atcgtgattg ctacaggtgc ccatccaagt attcctaata
    27481 ttcctggtgc tgagctaggt ggctcttctg atgatgtatt tgcctgggaa gaacttccag
    27541 agtcaattgc cattctaggc gctggttata tcgccgttga attagctggc gtactccaca
    27601 cttttggtgt caagacagat ctctttgttc gccgcgatcg tcctttacgt ggttttgatt
    27661 cctacatcgt tgaaggtttg gtcaaggaaa tggaaagaac aaacttacca cttcacactc
    27721 acaaagtccc tgtcaagtta gaaaaaacta ctgacggcat taccattcat ttcgaagatg
    27781 gtactagtca cacagctagc caagttatct gggctacagg tcgccgtcca aacgttaagg
    27841 gcttgcaact tgaaaaagct ggagtgactc tgaacgaacg tggctttatc caagtggatg
    27901 aataccaaaa tactgttgtt gagggaatct atgctctagg tgatgtaacg ggcgagaaag
    27961 aactgactcc agttgcaatc aaggccggac gtaccctatc tgaacgtctc tttaacggaa
    28021 aaactactgc aaaaatggat tactcaacta ttccaactgt tgtcttttca caccctgcta
    28081 tcggaactgt tggtttgaca gaagagcaag ctattaaaga atacggtcaa gaccaaatca
    28141 aggtttataa atcaagcttt gcatctatgt actctgcttg cactcgcaac cgtcaagaat
    28201 cccgtttcaa actcataaca gctggttcag aagaaaaagt tgtcggactt catggaattg
    28261 gctacggcgt tgatgaaatg attcagggat ttgccgttgc tatcaaaatg ggagcaacca
    28321 aggctgactt tgatgcaact gtggcaattc acccaactgc atctgaagaa tttgtaacca
    28381 tgcgttaatc gaaacaaaag aagctgatat aaatatgtct gcttcttttt
//
LOCUS       DAVBBS010000024        28408 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVBBS010000024 DAVBBS010000000
VERSION     DAVBBS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28408
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.024"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            380..1333
                     /locus_tag="VQU32_001021"
     CDS             380..1333
                     /locus_tag="VQU32_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2472488.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLISLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            1384..2574
                     /locus_tag="VQU32_001022"
     CDS             1384..2574
                     /locus_tag="VQU32_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HET2472489.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGTDAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            2723..3094
                     /gene="rnpA"
                     /locus_tag="VQU32_001023"
     CDS             2723..3094
                     /gene="rnpA"
                     /locus_tag="VQU32_001023"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET2472490.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            3069..3893
                     /locus_tag="VQU32_001024"
     CDS             3069..3893
                     /locus_tag="VQU32_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET2472491.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAKIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            3912..4898
                     /locus_tag="VQU32_001025"
     CDS             3912..4898
                     /locus_tag="VQU32_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HET2472492.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            4949..5572
                     /locus_tag="VQU32_001026"
     CDS             4949..5572
                     /locus_tag="VQU32_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HET2472493.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPGWQLQQTSQWMNQAQRLRPHFWVYLQRDEQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            5703..7160
                     /locus_tag="VQU32_001027"
     CDS             5703..7160
                     /locus_tag="VQU32_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2472494.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            7183..7464
                     /locus_tag="VQU32_001028"
     CDS             7183..7464
                     /locus_tag="VQU32_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2472495.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            7541..8026
                     /locus_tag="VQU32_001029"
     CDS             7541..8026
                     /locus_tag="VQU32_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2472496.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            8043..8708
                     /locus_tag="VQU32_001030"
     CDS             8043..8708
                     /locus_tag="VQU32_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HET2472497.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            8712..9575
                     /locus_tag="VQU32_001031"
     CDS             8712..9575
                     /locus_tag="VQU32_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HET2472498.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKEIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SRGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            9577..10281
                     /locus_tag="VQU32_001032"
     CDS             9577..10281
                     /locus_tag="VQU32_001032"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HET2472499.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVAWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            10460..12133
                     /locus_tag="VQU32_001033"
     CDS             10460..12133
                     /locus_tag="VQU32_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET2472500.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            12245..13336
                     /gene="ulaG"
                     /locus_tag="VQU32_001034"
     CDS             12245..13336
                     /gene="ulaG"
                     /locus_tag="VQU32_001034"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HET2472501.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            13450..15426
                     /gene="tkt"
                     /locus_tag="VQU32_001035"
     CDS             13450..15426
                     /gene="tkt"
                     /locus_tag="VQU32_001035"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET2472502.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVTDYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            15543..15842
                     /gene="yajC"
                     /locus_tag="VQU32_001036"
     CDS             15543..15842
                     /gene="yajC"
                     /locus_tag="VQU32_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET2472503.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVEGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            15889..16317
                     /locus_tag="VQU32_001037"
     CDS             15889..16317
                     /locus_tag="VQU32_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HET2472504.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            16319..16729
                     /locus_tag="VQU32_001038"
     CDS             16319..16729
                     /locus_tag="VQU32_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472505.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            17029..19680
                     /gene="adhE"
                     /locus_tag="VQU32_001039"
                     /gene_synonym="adhC"
     CDS             17029..19680
                     /gene="adhE"
                     /locus_tag="VQU32_001039"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HET2472506.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITEPSMEATSALMNHEGIATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
     gene            complement(20665..20973)
                     /locus_tag="VQU32_001040"
     CDS             complement(20665..20973)
                     /locus_tag="VQU32_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2472507.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
     gene            complement(20970..21278)
                     /locus_tag="VQU32_001041"
     CDS             complement(20970..21278)
                     /locus_tag="VQU32_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2472508.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            complement(21283..22578)
                     /locus_tag="VQU32_001042"
     CDS             complement(21283..22578)
                     /locus_tag="VQU32_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2472509.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTKFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPSAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGIVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            complement(22596..24005)
                     /locus_tag="VQU32_001043"
     CDS             complement(22596..24005)
                     /locus_tag="VQU32_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET2472510.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTEDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSLCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            24163..24891
                     /locus_tag="VQU32_001044"
     CDS             24163..24891
                     /locus_tag="VQU32_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2472511.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            25351..25545
                     /locus_tag="VQU32_001045"
     CDS             25351..25545
                     /locus_tag="VQU32_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2472512.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSDVTLITGKNGTGKSTLL"
     gene            complement(25911..27221)
                     /locus_tag="VQU32_001046"
     CDS             complement(25911..27221)
                     /locus_tag="VQU32_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET2472513.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNFAITNGTLDVLAQHILYRTRTYPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTSIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            27458..28330
                     /locus_tag="VQU32_001047"
     CDS             27458..28330
                     /locus_tag="VQU32_001047"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET2472514.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAILLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT         8903 a         5055 c         5921 g         8529 t
ORIGIN      
        1 gtgcattggt tagttgttta ctttcttcat aaattttatt gtatcatatt tttgtttcac
       61 aggaagtcta ataaataaaa attctttaaa attttgttgg gaaaatggga aaagatataa
      121 atggaaactt agtataacac ttggaaaact ctgtaaaaat agtaagtcaa ttcatgtccc
      181 gttttttctc tagggaagtt gggcggctat atttatatga ccatttgtcg taataaacat
      241 ccctaccaag ggtcaataga aataaattca acgaaacttt tgctgtcaga tttagatgcc
      301 caatttgtca tttatactct ttcatatagg actttctata aggatggaaa aaagaaggga
      361 aatttgataa gataggaata tggattttga aaaaattgaa caagcttata cctatttact
      421 agagaatgtc caagtcatcc aaagtgattt ggcgaccaac ttttatgacg ccttggtgga
      481 gcaaaacagc atctatctgg atggtgaaac tgagctaaac caggtcaaag acaacaatca
      541 ggcccttaag cgtttagcac tacgcaaaga agaatggctc aagacctacc agtttctctt
      601 gatgaaggct gggcaaacag aacccttgca ggccaatcac cagtttacac cggatgctat
      661 tgctttgctt ttggtgttta ttgtggaaga gttgtttaaa gaggaggaaa ttactatcct
      721 cgaaatgggt tctgggatgg gaattctagg cgctactttc ttgatctcgc ttgataaaaa
      781 ggtggattac ttgggaatgg aagtggatga tttgctgatt gatctggcag ctagcatggc
      841 agatgtaatt ggtttgcagg ctggctttgt ccaaggagat gccgttcgtc cacaaatgct
      901 caaagaaagc gatgtggtca tcagtgactt gcctgttggc tattatcctg atgatgccgt
      961 tgcgtcgcgc catcaagttg cttctagcca agaacatact tacgcccatc acttgctcat
     1021 ggaacaaggg cttaagtacc tcaagtcaga cggatacgct atttttctag ctccgagtga
     1081 tttgttgacc agtcctcaaa gtgatttgtt aaaagaatgg ctgaaagaag aggcgagtct
     1141 ggttgctatg attagtctgc ctgaaaatct ctttgctaat gctaaacaat ctaagactat
     1201 ttttatccta cagaagaaaa gtgaaatagc agtggagcct tttgtttatc cacttgctag
     1261 cttgcaagat gcaagtgttt taatgaaatt taaagaaaat tttcaaaaat ggactcaagg
     1321 tactgaaata taaaatagat tttgttataa tagttgaaaa cgcttaaaaa ggggtatcat
     1381 gttatgacaa aaacaattgc aatcaatgca ggaagttcaa gtttgaaatg gcaattatac
     1441 ttaatgccag aggaaaaagt gttagccaaa ggtttgattg aacgtatcgg tttgaaagat
     1501 tcaatttcaa ctgtaaaatt tgacggccgt tctgaacaac aaattttgga tatcgaaaat
     1561 catacacaag ctgttaaaat tttattggat gacttgattc gtttcgatat tatcaaggct
     1621 tatgacgaga ttacaggtgt tggccaccgt gttgtagcag gtggagaata tttcaaagaa
     1681 tcaacagttg ttgagggaga tgttttagaa aaagttgaag agttgagttt gttggctcct
     1741 ctacacaacc cggccaatgc agcaggtgtt cgtgctttca aggaattgtt gccagacatt
     1801 accagtgtag ttgtttttga tacttccttc cacacaagta tgccagagaa agcttatcgc
     1861 taccctctac caacaaaata ttacacagaa aacaaggttc gtaaatacgg tgctcatggt
     1921 acaagtcacc agtttgtagc aggagaagct gcaaaactct tgggacgtcc attagaagac
     1981 ttgaagttaa ttacctgtca tattggtaac ggaggctcaa ttacagctgt gaaagccggc
     2041 aaatctgtag acacttctat ggggttcact cctcttggtg gtattatgat gggaacgcgt
     2101 acaggggata ttgatccagc tatcattcct tatttaatgc aatatacaga ggattttaac
     2161 acaccagaag atatcagtcg tgttcttaac cgtgaatcag gtcttttggg agtttctgct
     2221 aattctagcg atatgcgcga tatagaagca gctgtagcag aagggaatca cgaggctagc
     2281 ttggcttatg aaatgtatgt tgaccgtatc caaaaacata tcggtcagta ccttgcagtg
     2341 ctaaatggaa cagatgccat tgttttcaca gcaggtgtcg gtgaaaatgc agagaatttc
     2401 cgtcgtgatg taatctcagg gatttcgtgg tttggttgtg atgttgatga tgaaaagaat
     2461 gtctttggcg ttacaggaga catctcaaca gaggcagcta aaatccgtgt cttggttatt
     2521 ccaacagatg aagaattagt cattgcccgt gacgttgaac gcttgaaaaa ataagtgaaa
     2581 ctaaaaaaat attcaataca aggagttggg aaagttattt ttccagcttc tttttctgat
     2641 gaaattgtcc aaaaccttgc tatgattggc ttttttgaaa aatatggtat aatagtagta
     2701 atttaataga tggagttgag ttttgaagaa aaactttcgt gtaaaaagag agaaagattt
     2761 taaggcgatt ttcaaggagg ggacaagttt tgctaatcgc aaatttgtgg tctaccaatt
     2821 agaaaaccag aaaaaccatt ttcgagtagg tctatcagtt agcaaaaaac tggggaatgc
     2881 cgtcactaga aatcaaatta agcgacggat tcggcatatt atccagaatg caaaagggag
     2941 tctggtagaa gatgtcgact ttgttgtcat tgctcgaaaa ggagtcgaaa ccttgggata
     3001 cgcagagatg gagaaaaatc tactccatgt attaaaatta tcaaagattt accgggaagg
     3061 aaatgggagt gaaaaagaaa ctaaagttga ctagtttgct aggactgtct ctgttaatca
     3121 tgacagcctg tgcgactaat ggggtaacta gcgatattac agccgaatcg gctgattttt
     3181 ggagtaaatt ggtttacttc tttgcgaaaa tcattcgctt tttatcgttt gatattagta
     3241 tcggagtggg gattattctc tttacggtct tgattcgtac agtcctcttg ccagtctttc
     3301 aggtgcaaat ggtggcttct aggaaaatgc aggaagctca gccacgcatt aaggcgcttc
     3361 gagaacaata tccaggtcga gatatggaaa gcagaaccaa actagagcag gaaatgcgta
     3421 aagtatttaa agaaatgggt gtcagacagt cagattctct ttggccgatt ttgattcaga
     3481 tgccggttat tttggccttg ttccaagccc tatcaagagt tgacttttta aagacaggtc
     3541 atttcttatg gattaacctt ggtagtgtgg atacaaccct tgttcttccg attttagcag
     3601 cagtattcac ctttttaagt acttggttgt ccaacaaagc tttgtctgag cgaaatggcg
     3661 ctacgactgc gatgatgtat gggattccag tcttgatttt tatctttgca gtttatgcgc
     3721 caggtggagt cgccctatac tggacagtgt ctaatgctta tcaagtcttg caaacctatt
     3781 tcttgaataa tccattcaag attatcgcag agcgcgaggc cgtagtacag gcacaaaaag
     3841 atttggaaaa tagaaaaaga aaagccaaga aaaaggctca gaaaacgaaa taaataagga
     3901 ggaatctggt aatggtagta tttacaggtt caactgttga agaagcaatc cagaaaggat
     3961 tgaaagaatt agatattcca agaatgaagg ctcatatcaa agtcatttct agggagaaaa
     4021 aaggctttct tggtctattt ggtaaaaaac cagcccaagt ggatattgaa gcgattagtg
     4081 aaacgactgt tgtcaaagca aatcaacagg tagtaaaagg cgttccgaaa aaaatcaatg
     4141 atttgaacga gcctgtgaag acggttagtg aagaaaccgt tgaccttggt catgtggttg
     4201 atgctattaa aaaaatagag gaagaaggtc aaggtatttc tgatgaagtc aaggctgaaa
     4261 tcttaaaaca tgaaagacat gccagcacta tcttagaaga aactggtcac attgagattt
     4321 taaatgaact tcaaatcgag gaagcgatga gggaagaagc aggcgctgat gaccttgaaa
     4381 ctgagcaaga tcaaactgaa aatcaagact tgaaagagat gggcttgaag gtcgagcaaa
     4441 gttatgatat tgcccaggtg gctacggatg tgactgccta tgttcaagcg attgtggatg
     4501 acatggatgt tgaagctaca ctttcaaatg attataaccg tcgtagcatc aatctacaaa
     4561 ttgacaccaa cgaaccaggt cgtattatcg gctaccatgg taaagtcttg aaggccttgc
     4621 aactgttggc tcaaaattat ctttacaacc gctattccaa aaccttctac gttacaatca
     4681 atgtcaatga ttatgtcgaa caccgtgcag aagtcttgca gacctatgcg caaaaattgg
     4741 cgattcgtgt tttagaagaa ggacgtagtc atcaaacaga tccaatgtcg aatagtgaac
     4801 gcaagattat ccaccgtatt atttcacgta tggatggcgt gaccagttac tctgaaggtg
     4861 atgagccaaa tcgctatgtc gttgtagata cagaataagt aaaatcaggg ttgacctgat
     4921 tttttgctag ctagaggagg ttaaactaat gttgaataag ataagagact atttagactt
     4981 tgctggtttg cagtaccgta atcctgataa agcgggagca gagcgagaga agatgctggc
     5041 attccgccac aaaggacaag aggcccgaaa ggcttttaca gaactggcca aagcctttca
     5101 agcaagccat ccaggatggc aactccaaca gactagtcag tggatgaatc aggcccagcg
     5161 tttgagacca catttttggg tttatctaca gagagacgaa caagtgacag aacctatgat
     5221 ggccttacgt ttgtatggga catctactga ctttggaatt tctttggaag tcagtttcat
     5281 cgaacgtaag aaggatgagc aaacactggg caagcaggcc aaagttttag acattccaac
     5341 cgttaaaggg atttattatc taacctactc taatggtcaa agtcaacggt gggaggcgaa
     5401 tgaagaaaag cgtcgtactt tacgcgagaa ggtgagaagt caagaagttc gaaaagtttt
     5461 agtgaaggta gatgttccta tgacagaaaa ttcgtctgaa gaagaaatcg tagaaggctt
     5521 attgaagtct tattctaaaa ttcttcccta ttatctagct acgagaaaat aagataattt
     5581 gtaaaacatc ataaatcata cagtccaaga gtgaacagtc cgctgtgtaa ttcttggtct
     5641 ttttgtttgc gctttcgcat tatataataa acttacaaaa acaattcaaa aggagaacaa
     5701 ttatggaagt cgtttcaagt gttctaaatt ggttttctag caatattttg cagaatcccg
     5761 catttttcgt aggtttattg gtgttgatag gatatgcact tttgaaaaaa cctgcccatg
     5821 acgttttttc agggtttgtt aaagcaacag tagggtatat gttgcttaac gtgggtgctg
     5881 gtggtttggt tacaaccttt cgtccaatct tagcagctct taactacaaa ttccaaattg
     5941 gtgcagcggt tatcgaccct tactttggac ttgctgcagc aaacaacaaa attgcagcag
     6001 agtttccaga ttttgttgga actgcaacta cagctctatt gattggtttt ggaataaata
     6061 tcttgctcgt agctcttcga aagattacga aggtaagaac cctctttatt actggtcaca
     6121 tcatggtaca acaagctgca acagtatctc ttatggttct attcttagta ccacaattgc
     6181 gcaatgctta cggtacagca gcgattggta tcatctgtgg actttactgg gcagttagtt
     6241 caaatatgac tgttgaggca actcaacgct tgactggtgg tggcggattt gcgattggtc
     6301 accaacagca atttgcaatc tggtttgtag ataaagtagc aggacgcttt ggtaagaaag
     6361 aagaaagttt agacaatctt aaattaccta agttcctctc aatcttccac gatacagttg
     6421 ttgcatctgc taccttgatg ctcgtattct tcggagccat tcttttaatc ttgggtccag
     6481 acattatgtc taataaagaa gtcatcactt caggaactct attcaatcct gctaaacaag
     6541 atttctttat gtacattatc caaacagcct ttaccttctc agtttacttg ttcgttttga
     6601 tgcaaggtgt ccgaatgttc gtatctgagt tgacaaacgc cttccaaggt atttcaaaca
     6661 aattgttgcc aggttcattc ccagcggttg acgttgcagc ttcttatgga tttggttctc
     6721 caaatgctgt cttgtcagga tttacctttg gtttgattgg tcaattgatt acaattgttt
     6781 tgctcatcgt ctttaaaaat ccgattctta ttattacagg atttgtacca gtgttctttg
     6841 acaatgcagc cattgcggtc tacgctgata aacgcggcgg atggaaagcg gctgttatcc
     6901 tttcctttat atcaggtgtc cttcaagttg ctctaggagc tctttgtgtg gcccttctcg
     6961 atttggcatc ttatggtggc taccatggaa atatcgactt tgaattccca tggcttggat
     7021 ttggatatat cttcaaatac cttggtattg ttggttatgt acttgtgtgt ctcttcttgc
     7081 ttgttattcc tcaacttcaa tttgccaaag caaaagataa agagaaatat tacaacggtg
     7141 aagttcaaga agaagcttag tatctagaaa aggagaaata aaatggttaa agtattagca
     7201 gcgtgcggaa atggaatggg ttcatcaatg gttatcaaga tgaaggttga aaatgctctc
     7261 cgtaagctta atcaaacaga ttttacagtc aattcatgca gtgtcggtga agctaaaggt
     7321 ttagcagtag gatatgacat tgtaatcgct tctcttcatt tgattcaaga attggaaggg
     7381 cgaactaatg ggaagttaat tgggcttgat aacttgatgg atgataaaga aatcaccgaa
     7441 aaactcagtc aagcactaca gtaaaaggtt ggagggggct ggacagaaac tgagagttat
     7501 cgtttctgtc cagccccctc tttaaataaa ggaggcagat atgaatttaa aacaagcttt
     7561 aattgacaat gactcgatcc gactaggttt agaggctaac aattggaaag aagcagtcaa
     7621 ggtagcagta gatcccttga ttgaaagtgg ggcaattttg ccagagtatt acgatgctat
     7681 cattgaatcg actgaagagt atgggcctta ctatatcttg atgccaggta tggctatgcc
     7741 ccacgctaga cctgaagctg gtgtgcaaag tgatgccttt tcattgatta ccttacaaaa
     7801 tcctgttgta ttttcagatg ggaaagaggt atctgttttg ttggcactag cagcaacaag
     7861 ttcaaaaatt cacacaagtg tagccattcc acaaattatt gccctgtttg aattagaaga
     7921 ttctattgca cgtttacagg cttgccagac taaagaagat gtcttggcta tgattgaaga
     7981 atctaaggat agcccttatc tcgaaggatt ggatttggaa agttagaaag aggaataaag
     8041 aaatgacaaa aagaatacct aatttacaag ttgcattaga ccattcagac ttgcaaggag
     8101 cgattaaagc agctgtttct gttggtcagg aagtagatat tatcgaagct ggaactgttt
     8161 gcttgcttca agttggaagt gaactggctg aagtcttgcg tagccttttc ccagataaga
     8221 ttattgtggc agacacaaaa tgtgctgatg ctggtggaac agttgctaaa aataatgcgg
     8281 ttcgtggagc agactggatg acttgtatct gttgtgcaac catccctact atggaagcag
     8341 ctctaaaggc tatcaagact gaacgaggag aacgaggcga aatccagatc gagctttatg
     8401 gcgattggac ttttgaacaa gctcagcttt ggctagatgc aggtatctca caagctattt
     8461 atcaccaatc tcgtgatgct cttcttgctg gtgaaacttg gggtgaaaaa gaccttaata
     8521 aggttaaaaa actcattgac atgggcttcc gtgtatctgt aacaggtggt ctagatgtag
     8581 atactctcaa actctttgaa ggtgttgatg tctttacctt tatcgcaggt cgtggaatta
     8641 cagaggctgc ggatccagca ggagcagcgc gtgccttcaa ggatgaaatc aaacgaattt
     8701 gggggtaaat catggtacgt ccaattggaa tttatgaaaa ggcaacccca acacacttta
     8761 cttggctaga acgtttaaat tttgccaagg agttaggctt tgattttgtc gagatgtcta
     8821 ttgacgaacg tgacgagcgt ttagcaagac ttgactggag taaggaagaa cgcttggaag
     8881 ttgtcaaaga aatctatgaa actggtgttc gtattccttc tatctgtttt tcaggccatc
     8941 gtcgctaccc attgggttca aaagatccag ttctagagga aaaatctcta gaactcatga
     9001 aaaaatgtat cgaattagct caagacttgg gagttcgtac gattcaatta gctggttacg
     9061 atgtttacta tgaggaaaag tcaccccaga cacgccaacg ttttatcaaa aatttgagaa
     9121 aagcctgtga ctgggctgaa gaagctcagg tggtacttgc tattgaaatt atggatgatc
     9181 ctttcatcaa tagcatcgaa aaatatttgg ctatagaaaa agagattgac tctcccttcc
     9241 tctttgtata tccagatatt ggtaatgtgt ctgcatggca taatgatatc tatagtgagt
     9301 tttatcttgg tcatcatgcc atcgcagctc tccatctcaa ggatacttat gcagtgacag
     9361 aaagttcaag gggccagttc cgagatgtac cttttgggca aggttgtgtc aaatgggaag
     9421 aagctttcga tattttaaag gaaaccaatt ataatggacc tttcctaatc gaaatgtggt
     9481 ctgaaaattg tgaaacagta gaagaaacac gcgcagccat tcaagaggcg caagcttttc
     9541 tctatccact cattaagaaa gcaggtttga tgtaagatga atcaagtaat caatgctatg
     9601 cgtaaacgag tctgtgatgc caatcaatca ttgccaaaac atggacttgt caaatttacc
     9661 tgggggaatg tatctgaagt caatcgcgaa ctcggtgtca ttgttatcaa accatcaggc
     9721 gtggattatg acgaattgac acctgaaaac atggtagtga ctgatctaga tggtaagatc
     9781 ctagaagggg atttaagacc atcttccgac ctcccaactc atgtgcaatt atataaggct
     9841 tggtcagaaa ttggtagtgt ggttcacacc cattcgacag aagctgttgc ttgggctcag
     9901 gcaggtcgtg atattccttt ctacggaaca acccatgcag attatttcta cggttcaatc
     9961 ccttgcgccc gtagtttgac caaggacgaa gtagaagtgg cctatgaaaa agatactggc
    10021 ctggttatcg tagaagagtt tgaacatcgc ggacttaacc cggttgaagt accaggaatt
    10081 gttgtacgca atcacggtcc attcacctgg ggcaaaaatc cagagaatgc tgtttatcac
    10141 tctgtcgtac tagaggaagt atcaaagatg aatcgcttta cagaacaaat caatccaaga
    10201 gttggacctg ctccccagta catactagaa aaacactacc aacgtaaaca tggaccaaat
    10261 gcttattatg gtcaaaagta agaacgatga aggaggagaa aaagataaat ttagctcctc
    10321 tttttacatt tgatttttat tgagagtaaa gttggagttg aagtaatttt aaaagatttt
    10381 ttagaaatag cgcttgatat atatatggta aaataaaaag aattgctgtg atatcaatag
    10441 atttggggga ttttttaata tggtactgga taaggcaagt tgtgatttgc ttcaatattt
    10501 gatggatcaa gaaacgtcca aaacgattat ggcgatttcg aaagatttga aagagtcaag
    10561 aaggaaaatt tattatcaca ttgacaaaat caatgctgct ctgggtgacg aggcgcttca
    10621 catcattagt attccacgaa ttggtattca cttaacggaa gagcagagag atgcttgttg
    10681 taaactatta tcggaagtag attcgtacga ttatatcatg agtgcgcatg aacgtatgat
    10741 gataatgtta ctatggatag gtatttctaa agaacgtatt acgattgaaa aattgataga
    10801 gttaacagag gtatctagga atactgttct caatgatttg aatagtattc gttatcaact
    10861 aactttggaa caatatcagg tgatcttgca agtgagcaag tcacagggat accaccttca
    10921 tgcccaccct cttaataaaa ttcagtatct tcaatcgctt ctatatcata tttttatgga
    10981 agaaaatgcc acttttgtat ctattttaga agataagatg aaagagaggt tagatgatga
    11041 gtgtttgctt tctgttgaaa tgaaccaatt ttttaaggaa caggttcctt tagttgaaca
    11101 agatttaggg aagaaaataa accatcatga aataactttt atgttgcagg ttctacctta
    11161 tttgctgtta agctgtcata atgttgaaca gtatcaagaa agacatcagg atatagagaa
    11221 agaattttct ttgataagaa aaagaataga gtatcaggtg tctaagaaat taggagaacg
    11281 gttgtttcaa aagtttgaaa tttctttgtc aggacttgaa gtttctcttg tagctgttct
    11341 cctcctctcc tatcgtaaag atttggatat tcatgcagaa agtgatgatt ttcggcaatt
    11401 aaaacttgct ttagaagaat ttatctggta ttttgaatca caaatccgaa tggagattga
    11461 gaacaaggat gatttgttac gaaatttgat gatccactgt aaagccttgt tatttagaaa
    11521 gacttacggt attttttcta aaaatcctct aacaaaacaa attcgatcca agtatggaga
    11581 attattttta gtcactagaa aatctgcgga aattttagaa ggagcatggt ttattcggct
    11641 aacagacgat gatattgcct atttgacgat tcatattgga ggatttttaa aatatacacc
    11701 atcatctcaa aaaaatatga aaaaagttta tctcgtttgt gatgaaggtg ttgcggtttc
    11761 gagacttttg ctgaaacaat gcaaacttta ttttccaaat gagcaaattg acactgtatt
    11821 tacaacagaa caatttaaga gtgtggaaga tattgcacaa gttgatgtag tgattactac
    11881 taatgatgat ttggatagca gatttccgat tttaagggtt aatcctatcc ttgaagcaga
    11941 agatattttg aaaatgctag actatcttaa acacaatata tttcgtaata agagcaaaag
    12001 tttcagtgaa aatctttcta gtcttatttc gtcttatatt gtagacagca agttggctag
    12061 taagttccaa gaagaggttc aaacacttat aaatcaagaa atagtagttc aagctttttt
    12121 ggaagatatt tgaaggacag tccaatgatg aacacaaacc tgtgtttttc ttggtctttt
    12181 ttagtgtttt gaagggtggt atactaatct caaagataac aattatatcc aaaggaggca
    12241 acatatgcca aacgtcaaag aaattacaag agagtcatgg attttagcca ctttcccaga
    12301 gtggggaaca tggttgaacg aagaaatcga agaagaagtc gtacctgaag gcaactttgc
    12361 catgtggtgg ctaggcaact gtggtacttg gattaagaca ccagctggtg ctaacgttgt
    12421 catggacctt tggtcaaacc gtggaaaatc aaccaaaaaa gtgaaagata tggttcgtgg
    12481 gcaccaaatg gcaaatatgg caggtgttcg taagctgcaa ccaaacttgc gtgttcagcc
    12541 aatggttatc gatccatttg ctatcaacga actagactat tacttagttt cacacttcca
    12601 cagtgatcat atcgacccat acacagctgc agcaattctc aataatccta agttagagca
    12661 tgttaagttt atcggtcctt accactgtgg acgaatctgg gaaggatggg gtgttccaaa
    12721 agaacgtatc atcgttgtta aaccaggtga cactatcgaa ttaaaagata tgaagattca
    12781 tgcagtagaa tcatttgacc gtacttgctt ggtaactctc ccagtgaacg gtgctgatga
    12841 gacaggcggt gaacttgctg gcttggctgt tacagatgaa gaaatggctc aaaaggctgt
    12901 taactatatc tttgaaacac caggtggaac catctatcat ggtgcagatt ctcacttctc
    12961 aaactatttt gcaaaacatg gtaaagactt taaaattgat gttgctttga ataactatgg
    13021 tgaaaatccg gtaggtatcc aagacaaaat gacatctatc gaccttcttc gtatggcaga
    13081 aaatctgcgt accaaagtca ttatcccagt tcactatgat atctggtcta acttcatggc
    13141 ttctactaat gagattctag aactttggaa aatgcgaaaa gatcgcttgc aatacgattt
    13201 ccatccattt atctgggaag ttggcggtaa gtacacttat cctcaagatc aacacttagt
    13261 agaataccat catccacgtg gttttgatga ttgttttgaa caagactcta acattcaatt
    13321 taaagctttg ctataatata aaaatattct ttggaggtta tccgtatgtt gcatgatacg
    13381 gataattttc atataatagt aaaatagaat gtgtgattca ataatcacct caaatagaaa
    13441 ggaaattcta tgtcaaatct atctgttaat gcaattcgtt ttctaggtat tgacgccatt
    13501 aataaagcca actcaggtca tccaggtgtg gttatgggag cggctccgat ggcttacagc
    13561 ctctttacaa aacaacttca tatcaatcca gctcaaccaa actggattaa ccgcgaccgc
    13621 tttattcttt cagcaggtca tggttcaatg ctcctttatg ctcttcttca cctttctggt
    13681 tttgaagatg tcagcatgga tgagattaag agtttccgtc aatggggttc aaaaacacca
    13741 ggtcacccag aatttggtca tacggcaggg attgatgcta cgacaggtcc tctagggcaa
    13801 gggatttcaa ctgctactgg ttttgcccaa gcagaacgtt tcttggcagc caaatataac
    13861 cgtgaaggtt acaatatctt tgaccactat acttacgtta tctgtggaga cggagacttg
    13921 atggaaggtg tctcaagcga ggcagcttca tacgcaggct tgcaaaaact tgataagttg
    13981 gttgttcttt atgattcaaa tgatatcaac ttggatggtg agacaaagga ttcctttaca
    14041 gaaagtgttc gtgaccgtta caatgcctac ggttggcata ctgccttggt tgaaaatgga
    14101 acagacttgg aagccatcca tgctgctatc gaaacagcaa aagcttcagg caagccatct
    14161 ttgattgaag tgaagacggt tattggatac ggttctccaa acaaacaagg aactaatgct
    14221 gtacacggcg cccctcttgg agcagatgaa actgcatcaa ctcgtcaagc cctcggttgg
    14281 gactacgaac catttgaaat tccagaacaa gtatatgctg atttcaaaga acatgttgca
    14341 gaccgtggcg catcagctta tcaagcttgg actaaattag ttacagatta taaagaagct
    14401 catccagaac tggctgcaga agtagaagcc atcatcgacg gacgtgatcc agtcgaagtg
    14461 actccagcag acttcccagc tttagaaaat ggtttttctc aagcaactcg taattcaagt
    14521 caagatgcct tgaatgttgt ggcagctaaa ttaccaacct ttctaggtgg atcagctgac
    14581 cttgctcact caaacatgac ttatatcaag acggatggac ttcaagacga tgccaatcgc
    14641 ttgaatcgta acattcagtt tggtgttcgt gaatttgcaa tgggaacaat cttgaacggg
    14701 atggcccttc atggtggact tcgtgtatac ggtggaactt tcttcgtctt ctctgactat
    14761 gtgaaggcag ctgtccgctt gtcagcctta caaggacttc ctgtgactta tgtctttacc
    14821 catgattcaa tcgcagttgg ggaagatggt ccgactcatg aaccagttga gcatttagca
    14881 ggtcttcgtg ctatgccaaa tctaaatgtt ttccgtccag cagatgcgcg tgaaacgcaa
    14941 gcagcttggt accttgcagt gacaagtgag aaaacaccaa ctgcccttgt cttgacacgt
    15001 caaaatttga ctgttgaaga tggaacagac ttcgacaagg ttgctaaagg tgcttatgtt
    15061 gtatatgaaa atgcagccga ctttgatacc atcttgattg cgacaggttc agaggttaat
    15121 cttgctgtct cagctgctaa agaattggct agtcaaggcg aaaaaatccg cgtagtcagc
    15181 atgccatcta cagatgtctt tgataaacaa gatgcagctt acaaggaaga aattcttcca
    15241 aatgcagtcc gccgtcgtgt tgcagtcgaa atgggtgcaa gtcaaaactg gtacaaatat
    15301 gttggtctcg atggtgccgt tctaggtatt gataccttcg gagcctctgc cccagcacca
    15361 aaagtattgg cagaatatgg ctttactgta gaaaatcttg taaaagttgt tcgaaacttg
    15421 aaataatcct aaaaatcagg gcgtaagctc tggtttttct taccagaaaa gtaaggtaca
    15481 atcttgtaaa agtagctgaa atttgatata gtagtcctat gtaaaagaca aaggagaaaa
    15541 caatgaatcc aaatattact tttttaatca tgcttgtagg tatgatggcc ttgatgttct
    15601 ttatgcaacg ttctcaaaag aaacaagctc aaaagcgtat ggaaagctta aataaactac
    15661 aaaaaggcta tgaagtgatt acaatcggtg gactttatgg aacagtcgat gaagtagata
    15721 cggagaaggg aacaatagtt cttgacgtag agggagttta cttgactttt gaactagctg
    15781 ctatcaagac agtattaccg ctgaaagaaa cagcttcact agaaggcgca attgaaaaat
    15841 aagacgggat cacgaactcc cgtttttcta taaaagaaag gaaatgggat gaaaaaatta
    15901 gtctttgtct gtctgggaaa tatttgccgt agccctatgg ccgagtttgt tatgaaatca
    15961 atgacagata actacgaaat ccaaagtcga gcaacttcct cttgggaaca tggcaatccg
    16021 attcataagg ggactcaggg aatttttcaa gagtatgaga ttccttatga caagaacaag
    16081 acatcgcttc agattagtaa ggaagatttt gaagcctttg attatattat cggaatggac
    16141 gcttcaaata tttctgactt acgtcagatg tgtccagtag actgtcaaga taagatttac
    16201 tcattttcat ctgaaagtgt tccagaccct tggtatacag gagattttga agaaacctat
    16261 cgacgtgttc aagagggctg tcaagcttgg ttagaacgtt tagaaaagga gagttaaggt
    16321 ggaaaatctt acgaattttt acgaaaagta tcgtgtctat ctgactcgtc cacgtttaga
    16381 gcttttggca gtagttacca ttgttttctg tgctgtactc gtcttttttc taaatattcc
    16441 aggaaaaggt gtcttaaaac tcgataatgg aacgattgtt tatgatggca gtcttgtccg
    16501 cggtaaaatg aatggccaag gtaccattac cttccaaaat ggagaccaat atacaggtgg
    16561 cttcaacaat ggagccttca acggaaaagg tacctttcaa tctaaagaag gctggaccta
    16621 cgaaggtgat tttgtaaatg gtcaggctga aggaaaaggg aaactaacaa cagaacaaga
    16681 agtcgtttat gaaggaactt ttaaacaagg cgtttttcaa caaaaataaa gcctccttat
    16741 caaaggaggt attattagaa tcgcaaggta agcgcttacc tgcaaatccc tttctttcca
    16801 aatccctctt ccaagcaagt ttgtgaaata aaaaatattt gaaataaatt tcacaaactt
    16861 caaagataaa acctgataag aaaagaaaat gagaaaagtt tcacaagagt ttaaaaattc
    16921 tttgtgaaat gtttatgaaa ctgtttacaa agttgaaata atgcgttata ataaacttgt
    16981 gaaaaaatta acaaaggata tcgttccttg aaagctatgg aggaaaatat ggctgataaa
    17041 aaaactgtga caccagagga aaagaaactc gttgctgaaa aacacgtaga tgagttggtt
    17101 caaaaagctc tagttgccct tgaagaaatg cgtaaattgg atcaagaaca agttgactac
    17161 atcgttgcca aagcatcagt agcagctttg gatgcccacg gagaattggc tttacatgcc
    17221 tttgaagaaa caggacgtgg tgtatttgaa gataaagcaa ctaagaactt gtttgcttgt
    17281 gaacacgtgg taaacaacat gcgtcacact aaaacagttg gcgttatcga agaagatgat
    17341 gtaacaggat tgaccctgat tgctgaacca gttggtgttg tttgtggtat cactccaaca
    17401 acaaacccaa catcaacagc gatcttcaaa tcattgattt cattgaagac acgtaatcca
    17461 atcgtctttg ccttccatcc atcagctcaa gaatcatcag ctcatgcagc tcgtatcgtc
    17521 cgcgatgcag ctatcgcagc tggtgctcct gaaaactgtg tacagtggat cactgaacca
    17581 tctatggaag caactagtgc ccttatgaac cacgaaggta ttgcaacaat ccttgcaaca
    17641 ggtggtaatg ccatggttaa agccgcatac tcatgtggta aaccagctct tggggtaggt
    17701 gccggaaacg ttccagctta tgttgaaaaa tcagcaaaca ttcgtcaagc agcacacgat
    17761 atcgtcatgt ctaaatcatt tgataacggt atggtctgtg catctgaaca agcagttatc
    17821 attgataaag aaatttacga tgaatttgta gcagagttca aatcttacca cacttacttt
    17881 gtaaacaaaa aagaaaaagc tcttcttgaa gagttctgct tcggcgtcaa agcaaacagc
    17941 aaaaactgtg ctggtgcaaa attgaacgct gacatcgttg gtaaaccagc aacttggatt
    18001 gcagaacaag caggatttac agttccagaa ggaacaaaca ttcttgctgc agaatgtaaa
    18061 gaagttggcg aaaatgagcc attgactcgt gaaaaattgt caccagttat tgcagttttg
    18121 aaatctgaaa gccgtgaaga tggtattact aaggctcgtc aaatggttga atttaacggt
    18181 cttggacact cagcagctat ccacacagct gacgaagaat tgactaaaga atttggtaaa
    18241 gctgttaaag ctattcgtgt tatctgtaac tcaccttcta cttttggtgg tatcggggac
    18301 gtttacaatg ccttcttgcc atcattgaca cttggatgtg gttcttacgg acgcaactca
    18361 gttggggata acgttagtgc cattaacctc ttgaatatca aaaaagtcgg aagacggaga
    18421 aataacatgc aatggatgaa acttccttca aaaacatact ttgaacgtga ttcaattcaa
    18481 taccttcaaa aatgtcgtga cgttgaacgt gtcatgatcg ttactgacca tgccatggta
    18541 gagcttggtt tccttgatcg tatcatcgaa caactggacc ttcgtcgcaa taaggttgtt
    18601 taccaaatct ttgcggatgt agaaccggat ccagatatca caactgtaaa ccgtggtact
    18661 gagattatgc gtgccttcaa accagatacc atcatcgcac tcggtggtgg gtctccaatg
    18721 gatgctgcca aagtaatgtg gctcttctac gagcaaccag aagtggactt ccgtgacctt
    18781 gtccaaaaat tcatggatat ccgtaaacgt gccttcaagt tcccattgct tggtaagaag
    18841 actaaattca tcgcgattcc aactacatct ggtacaggat ctgaagtaac accatttgcc
    18901 gttatctctg ataaagcaaa caaccgtaaa tacccaatcg ctgactactc attgacacca
    18961 actgtggcaa tcgtagatcc tgctttggta ttgacagttc caggatttgt tgctgctgat
    19021 actggtatgg acgtattgac tcacgcgaca gaagcatacg tatcacaaat ggctagtgac
    19081 tacactgatg gtttagcact tcaagccatt aaattggtct ttgaaaatct cgaaagctca
    19141 gttaagaatg cagacttcca ctcacgtgag aaaatgcata acgcttcaac aatcgctggt
    19201 atggcctttg ccaatgcctt cctaggtatt tctcactcaa tggcccataa gattggtgcg
    19261 caattccaca caatccacgg tcgtacaaat gctatcttgc ttccatacgt tatccgttac
    19321 aacggtacac gtccagctaa gacagcaaca tggcctaagt acaactacta ccgtgcagat
    19381 gaaaaatacc aagatatcgc acgcatgctt ggacttccag cttctactcc agaagaaggg
    19441 gttgaatctt acgcaaaagc tgtctacgaa ctcggtgaac gtattgggat ccaaatgaat
    19501 tttagagacc aaggaattga cgaaaaagaa tggaaagaac attctcgtga attagccttc
    19561 ctggcttatg aagaccaatg ttcaccagct aacccacgtc ttccaatggt agaccatatg
    19621 caagaaatca tcgaagatgc atactatggc tacaaagaaa gaccaggacg ccgtaaataa
    19681 ttgtttatca gtctagaagc aagacaaaaa ctcaatttga gggaaagatc cagtaatttt
    19741 tctatgataa aaggcatcct atcaaggttt ttgaacacct gataggatgc ctttttatga
    19801 tattgaggcc tttttgccct ttttgaaaaa ctagaataga aacaaaatat ataatagatt
    19861 gaaactggaa tagtacactt ctgcttctaa aacattgtta gaattcgatt tgactgtcct
    19921 gatcgatttg tcctattcct atttcatttt actatataaa aaatatagta tagtagactg
    19981 aatctaaaat agtacgaaac aattgctaaa acatttatag aaattaattt tactttcctg
    20041 atagagttgt tcacatctta tttcaattca ctatagttta atttaagagt agtatttact
    20101 aaggcccaat taaaatcaaa gagcaaacta gaaaacgagt gccattcagc tcaaaacact
    20161 gatttgagat tgcagataag actagcccct cattaacagg tttacgataa aacgatgaca
    20221 aggtgtgttg ctttttgatt tctaaagagt ataatgatag atctctataa aataagtgcg
    20281 aaggaaatga gcttttatag tcctttcgtt ttaaaatact atctcagatg ttcttatatc
    20341 gacaagaagt ttttgagtca ttccctcatc atacatatta aataaatagt ggctcattca
    20401 attttttact agaataataa gctagtatag taaactgaaa taagatataa acaaataaat
    20461 tggagcttaa catctatttc cagcaatttt ttagaaactg cagtggacta ttctaaattc
    20521 aacacattat aaaaactaga gtaaaagaaa aggattggat cttgtgtaat gcaggatcta
    20581 atcctttcaa tcattttgtc caacttttgg aggttcctac aatgtagccg tcattaataa
    20641 agacagatgg gaacgacagt gttcctattt attttgatag agatcgatga attctttaga
    20701 tagcaactga ataatctctg ttgaagccat ttggtcttct gcatgcataa atagcaagga
    20761 gaatcctatt ttttctccag tagcttcttt ttgtatgaga ttagagtgaa tcttgtgcgc
    20821 ttctactaag gagtcttccg cttcttcaac tttaattttc gcttctttta aatttcctgc
    20881 cttagctagt tggatggctt caataaagga tgatttggct gctccactat tggcaatgag
    20941 ctgaaaacag atatattcca tttcttctgt catcttattt ctcctatcca tgcaagtgct
    21001 tgttccagaa cttttgctcc attcatcatt ccgtaatccc gcatatcaat ggtatctaca
    21061 gggatatttc ctgcaatttc tttcacagca agtaactcat aacgaatttg tggcccaatt
    21121 agaatgacat ctgcttcatg gatattcttt ttcgcttctg tcattgattt tgcttggata
    21181 gaaatttcaa tcccacgttc agtcgcactt tgttgcattt ttttaacaag catacttgtc
    21241 gacattcccg cattacatac taataaaatt tgtttcataa tcttaacctt ccatttcttg
    21301 ctcaacaact ttgtcattaa ctttgataaa tggaatgtat agaagaactc caagtgcaaa
    21361 gatgatgaat tgaactagaa ctgctctcac gtcccctgct gttgctaacc atgcatttaa
    21421 gaatactggt gtagtccaag gaacttgtat aaatgcagga ctcatgaatc ctgtaactgt
    21481 tgctaagtag ctgattaaaa taccaaggac tggaactgtg ataaatggaa tagctaatga
    21541 aatgttataa acgattgggt aaccgaataa tactggttca ttgatattga agataccagg
    21601 tccaaaagat aatttagcca cgtttttaga aacagcattg cgactcacta agaatgttgc
    21661 tattaataaa cataatgtag atccactacc acccattaaa gcgaatgttt gtatttgtga
    21721 taagttgatg atgtgtggaa tggcttgtcc attatttgct gcagtgatgt tttcagtaat
    21781 gttaattaat agtaatggtt ctaggatggc actgtaaata actgcttggt gaataccaaa
    21841 tagccataac atatttccta aagagtaaat aataatgacc ccgattaagc ttgtaccaat
    21901 atgacgaatt ggttcttgaa taaagattgt aatgattgag attaagttca ttccagttat
    21961 attgaataat aatgctgaaa caatcccaaa taaggagatg acagtcatga ctggaagtaa
    22021 tacgctaaat gatctactaa cagctggtgg aatattttca ccaaggttca tttgtaaagc
    22081 tttaacgttt gataattcaa tgaataattc tgttgcaata atcgctacga taaccccggc
    22141 gaacattgcg cctgtacctg tgttgttgaa tgaaagaaca cctgaaatgt ttacagcatc
    22201 ttttgctccg tcaggaacta cagaaactgt atttggcatc atcacaatta aagaaactaa
    22261 tgatagcatt gatgctgctg acgggttttc gaaatctctg tttttagcta agaaataacc
    22321 aaccattaca gcaataatca tacctgaaat acttaaagta ccgtttgcaa ttgttattcc
    22381 ccaatattgg aattttgtta atgtatcccc ttggaaaatc cacttaaata ccgtgttgtt
    22441 caaaagaacg attaaacctg ccaaaatata taatggcatt actgttacga atgcatctct
    22501 tagggttttt aaatgaattt ggttccctag tttaccagca aaggtggcaa attgatctat
    22561 aaattttgag tttttcatct gaatatctcc tctctttact cctcaatttc tacttcaaaa
    22621 ccgttattgt cacttacttt gcgataccat cttcctgagc tcttgatggt tctcttttgc
    22681 gtttctaaat caacggagat aaatccgtaa cgattcttat atgcattatt ccaagaccag
    22741 caatcaaatg cggtccaagc gtggtatcca aaacagagac ttccctcttc aatggcttta
    22801 tgtagccatc ttaaatgttc ttcataaaat tcaatacgat acacgtcatc gataactcca
    22861 ttttcatcga taaaccgtgc ttcgttttca acacccattc cgttctcact gataaaccat
    22921 gggatattac catattcttc cttcacaatc atagcaatat cataaatggc tttcggaaaa
    22981 atttcccaac cacgatatgg attcatgcga cgctccggcc attcatactc tttgaagtat
    23041 tggtctggca tccagggcgt ctgatattcc tcaggatttg cttgtgcttg aacacgtttt
    23101 ggatggtagt agtttactcc aagaaaatca accgtatttg atttcatcag ttgaagctct
    23161 ttttcggtat gactccataa cacgccatct ctctctagct gttttaccaa tctttctggg
    23221 aaagttcctt taacagctgg attcaagaag actttgttaa agaagtcttc tgtaaatcga
    23281 cttgcttcta agtcttctgg agaattactt cttggataag caggtgtcaa gtttaaaata
    23341 atcccaatct ttccatcaag tcctaatgag cgatatagtt gaatcacttt tgcactagca
    23401 aggtttagat tatagatgac ttgtacggcc tcttttccct ttccttttag atttggataa
    23461 tggaaagcat ataagtatcc tgcttctgga atgaccattg gctcattgaa agttgtccag
    23521 taatgaacct tatctccaaa gcatgtgaaa gcagtcttgg caaacttcac gaataactcc
    23581 actacatgtt tgctttccca accaccgtat ttttgaagaa gttccactgg taaatcaaaa
    23641 tgatgtaaat tcatcacaag atccatctgg ttctttttag cttcttcaat gatggcattg
    23701 tagaaagcaa taccttttgg atcaggctca cctgtctcta aattcttgat gagtcgactc
    23761 cattggatgg aagtgcggaa agaattaact ccaatttcct tcatcaagtg gaaatcttcg
    23821 gtgtaagtat gaaaaaagtt actggctaca agaggtccga cattatcgaa aaaatcttct
    23881 ggatgcgttt tgaaccagta gtccatcaca ttttcatgta cttttccata ttgaccttct
    23941 gtctgaggtc cgctagaagc agctccccac caaaacagat taggaaatac taactttttt
    24001 ttcatgctca acctcctttc tattatgcta tacataaagt atagcataat tttaaaattt
    24061 gtctagacat ttttaaaaat tgttgataca tatattgtgt ttgcctcatt tcttgaactt
    24121 tgttatacta ctattagaat attatagaga gaaccagatt aaatgagtac aaaatattta
    24181 tttatttaca atgagattcg tgaaaagatt ctctgtaata aatataccat gaacgaacaa
    24241 ttgcctgatg aaatgacatt agctaaacag tttgcctgta gtcgaatgac gatcaaaaaa
    24301 gctttagact tgttagtttc tgagggctta atttttagaa aacgtgggca gggaaccttt
    24361 gttctctctc gtggcagctc aaaaagaaaa ttaatcgttc cagaaagaga tatccgggga
    24421 ctgacaaaaa tatctgaaga tgctcattct acaattgact cgaggattat tcacttcaaa
    24481 ttagaatttg caaatgaatt tttagcagaa aaactacagg tcgctttgca gagtccagtt
    24541 tataatattt accgcctgcg tattattgac ggtaaacctt atgttctgga acaaacttat
    24601 atgagtaccg atgttattcc aggtattact gaagatattt tacaaaaatc gatttacaat
    24661 tacattgaag gaaagttagg attgcatatt gccagtgcta caaaaatctt gcgagcttct
    24721 tctagttcag aaaatgagca acattacttg cagctccttc caacagaacc ggtatttgaa
    24781 gtagaacaag tggcttattt ggataacgga actccgtttg agtactcgat tagtcgtcat
    24841 cgctatgatt tatttgaatt taattctttt gcattacgac attcctccta ggagaaaatg
    24901 tgaaaatgaa gccaatcttt tacaggctct agtttaagaa aaatttaaaa cagggcaaga
    24961 aggtcccatc tatgcttaaa tggtttctct tttctaaata agatggcttt aaaagagtga
    25021 tcgttttatc catcatgttg aaaaatatct tcgtatagct tatagagtag gtactgaaat
    25081 tgttcacctg atctacttct tatagttatt tagttttaaa tagtgtttca aacattctta
    25141 cactgacgag aagtttttga gtcttttctt gtaacacata tagtatactg aggttagaat
    25201 agtagactgt gacttctaac aaattgctag aaatgaattt caatctccca atttatttgc
    25261 ttatatatta ttttaatata ttaaataaat tctggatcat aatcatttaa aaaaatttta
    25321 tttttttatt tttcattacg aataatatag atgaagggga aagagtatga aaacagaact
    25381 gtttcttttg ctattagttc aaaaggagaa aaaatgaaag tagaaaatat ttcgtatagg
    25441 gtggatcatc gtatattgtt tgataatatt tcttttgata cttcgagttc agacgtgaca
    25501 ttaattactg gtaaaaatgg tacaggaaag tcaactttac tatagtggat tgaaactaga
    25561 atagtacaca tctacttcta aaatattgtt agaaatcgat ttgactatcc tgatctattt
    25621 gtcctgttct tatttcattt cactatatct caaattgagt atgacgaagt gcgctcccat
    25681 gtcctgggaa cgcactttct tcatattttt catattcttg aatccatcga taaagactat
    25741 tgggatgaat ttttaaagtt gaactaatca tttttacagg atgaggttta cagcagagag
    25801 tttgaaggct ttatcaaagg tttttcttgg cataatgact tttcctcgtt tccacttaat
    25861 tttgtgtcta ctttattata ccaagtccac ccttaagtta gataataaat ctaacttaag
    25921 gaagctagaa ggatgagaat ccaggtggtc aagagtccca aacttaagct gattgggaca
    25981 cccagaataa tttgcttttt gaaggcaagg ccacgttcct ctatattggg aagtgagagt
    26041 tgaatgagag aaccagctga tgaaaagggt gagatattag tagatagagc gccaataacg
    26101 gtggctgttg tgagtaagtg aatatcaatc tggggatttt gagcactgat gatagcaatg
    26161 atgggaaaga gggctggagc tacaacggat agggtggaac taaagagtga catcaccccg
    26221 gctatcacac aaaagaacag aggtaaccag aaatgaggaa tggatgttgt catgaggtgc
    26281 cctatcagtg tgactaaacc tgacttgacc gctagagaca ttagtaagct catgccgcag
    26341 agcatgataa ttgtagccca gggaacttta gccaaaatgg cttcttgctt ccctaatttg
    26401 agccttaagg cgaggcagac catgagtatt gagacaaagc caatatcaaa tgtttttcga
    26461 taagtagcta tccaggcgat gtttgggaaa atgagatgca acaagggaaa aagccaaacc
    26521 aaaaccatgc tgctgatcat gagcaaggtg gtttgtcttt gaaccttgct gaggagtggt
    26581 ggttggtcaa tagtcaagga tgagtttgtt cttcccttac tatagtgact gtaacaggat
    26641 aataaaagca agacaatgag tgggtagata atgctgacga taaagatatg attgccaagt
    26701 gaaaaagctt gctcttccca tcccatttgc ttaaacaggc cttgaaagac aatgcctgag
    26761 ctactggtta tcaaattagc ccctcctgaa gctccccaat tgacggcttg agctccaatc
    26821 aaagggtgtt tgtccgcttt ttgacagaga gtaatcgcta gaggacagca aacggccata
    26881 gtagtgaaaa atccagcacc taaagcagac aaaagggttg ccatcaggta taaaatcatg
    26941 tagagggcgt tagggtaggt gcgtgtgcgg tagagaatgt gttgagccaa aacatcaaga
    27001 gtaccgttag ttattgcaaa gttataaaag agagagacgc taaaaatggt aaaaaagagt
    27061 gaggttggcc aaaaatgaag aagttctttg gggcttaatc ccatgagagt ggttgcgatg
    27121 aggtaagaaa aagcaatagc cagcaggcca atattgattt tggtgcggta accaattcca
    27181 atggctagag caatggcgct aatcattatt aaatgaatca ttgaattgtc ctttcagtta
    27241 gaatatggaa ataggttggc aattttggcg ctcaaaagac cacctgtttc ctattggtac
    27301 ttgactatta tacacaaaaa aaatacaatt gtcttgacaa ttgtattgac acttgttaga
    27361 attaaaaatg aggtattcag gcttgtgatt tcctatggaa gttaatagtg attgcctcta
    27421 atgcttacaa gtgatattaa aaatagagga cctagtgatg tcaatcattt caactgattt
    27481 aacccctttt caaatagatg atacattgaa agcagccttg cgagaagatg ttcattccga
    27541 agattacagt accaatgcca tttttgatca tcatggccaa gccaaggtgt cgctttttgc
    27601 caaggaagct ggtgttttag cggggctaac cgtttttcaa agggttttta ccctatttga
    27661 tgccgaggtg accttccaga atcctcatca atttaaggat ggggatcgtt tgactagtgg
    27721 cgatttggtt ttagaaatca taggctcggt gagaagtctc ttaacatgtg aacgcgttgc
    27781 cttgaatttt ttacaacatt tatcagggat tgcttcgatg acagctgctt atgtagaagc
    27841 cttaggcgat gattgcatta aggtatttga tactcgaaaa actactccta atttacgtct
    27901 ttttgagaaa tatgcagtga gagttggcgg tggctataat catcgcttta atttatcaga
    27961 tgctatcctg ctaaaagaca atcacattgc ggcagtaggt agtgttcaaa aggcaattgc
    28021 tcaagcgcgt gcctatgccc cttttgtgaa aatggtcgag gtggaagtgg aaagccttgc
    28081 tgctgccgaa gaagctgcgg cggcgggtgc tgatattatc atgttggata atatgtcatt
    28141 ggaacagatt gaacaggcca ttaccctaat tgcaggacgt tctcggattg aatgttctgg
    28201 aaatattgat atgaccacta ttagccgttt tcgtggttta gcgattgatt acgtctccag
    28261 tggtagttta acccatagtg ctaagagtct tgatttttcc atgaaggggt taacctacct
    28321 tgatgtctaa gttgtaaaat aaactaactt tttaaaggat gtctttcctc tagaacgagt
    28381 tttatgtcag atagtttaaa cgcctctt
//
LOCUS       DAVBBS010000025        27275 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVBBS010000025 DAVBBS010000000
VERSION     DAVBBS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27275
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.025"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            228..1910
                     /locus_tag="VQU32_001048"
     CDS             228..1910
                     /locus_tag="VQU32_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2472515.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNMSIS
                     KGQVQLQNELVKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHRKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            1895..2725
                     /locus_tag="VQU32_001049"
     CDS             1895..2725
                     /locus_tag="VQU32_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2472516.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVASLLLSLQNQGRFYNPWK
                     "
     gene            2918..3421
                     /locus_tag="VQU32_001050"
     CDS             2918..3421
                     /locus_tag="VQU32_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HET2472517.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     regulatory      3475..3709
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3874..4437
                     /locus_tag="VQU32_001051"
     CDS             3874..4437
                     /locus_tag="VQU32_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2472518.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTVAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYILLKPTLKHYER"
     gene            4451..5077
                     /locus_tag="VQU32_001052"
     CDS             4451..5077
                     /locus_tag="VQU32_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET2472519.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRSLTRLIDIPVIITWVVIIAFVFYRKRWKIESFFMLGNLTLAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGALFIEFPFYDKLRFQWRFKGKQK"
     gene            5189..6061
                     /locus_tag="VQU32_001053"
     CDS             5189..6061
                     /locus_tag="VQU32_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000525616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2472520.1"
                     /translation="MGMLLATRLKNKRKELKLSQKELAEGICEQGQISRMEQGKYSPG
                     SELLFQLSKRLKVSMDYFFEDTEVSSLENIDKFKELSKKFLDEREYESLHYIYELEKS
                     KRARLSAKDQIYLDWIDTLVQFCYLNHQTGAVAKLEKILLSLKKSDFYYLQLGNTLAN
                     FYFDMGQEDTYQELYQLLKENLQSLSVHHLEQLELMIKFRYNYCRYLWLNERTSEAIE
                     EITETIAICKRYNSSYRLADLYCLLGSISEGFAERDRILGFYQVSYQLYKHEENQRMM
                     LEIEKEMKLLESED"
     gene            6191..6328
                     /locus_tag="VQU32_001054"
     CDS             6191..6328
                     /locus_tag="VQU32_001054"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472521.1"
                     /translation="MKRSQVVKRCILIALLLLFLLNVLSGSLSVTTRGVDPRPAYGVS
                     L"
     gene            6752..7666
                     /locus_tag="VQU32_001055"
     CDS             6752..7666
                     /locus_tag="VQU32_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HET2472522.1"
                     /translation="MKLYQINDGIQIFESSNLEKIVIRNYRNGKKITIDHQPLLKKLL
                     LFLEVPKREEEILLYLPELSNKTLRSVLTNFMSLNIIHCQDTLIIHKKINILIIGLGT
                     TGGYLADGLCRSNINSKLFLLDKDRVDETNVGRQIYLRDDIGQYKVDVFKQKFSDKEI
                     KVFKIFINSKKDLEKICKREGIDLVIQCGDSPSPRELGKIVNTVCDTLQIPYIVNTGY
                     MSNVVPLPEFYYPNNRYNFNYKHQFSDEKLLFSQVLSKADYMVAIQSSFVMLMQIKNF
                     IFNEEPIYYGYRGYYNSVTLAWEVEKID"
     gene            7659..8672
                     /locus_tag="VQU32_001056"
     CDS             7659..8672
                     /locus_tag="VQU32_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472523.1"
                     /translation="MTKYLELDYSHSYILEGFRKNDAFIENHKECLDMLSDKIWRDNK
                     YMNTEKNISSPTRTILSRYTCNILSSLFIDISKNSIEKLIVTCENSDLNWIYNYYKSV
                     YNGVFCELRQLIQQYCKVKDKYEKWLFIKAIINQGIRQNEKEVVEFFLKQLKDNNQGI
                     FDYINSFSFYLLKFYSKDKSRIFLEEQLDIDDFLGSDKEDYARSLVFKNYASLLPDTS
                     ELKRNIMEKCLSQTPQDTDLWKEWFETYASQKEIRQKATEIFKHGYSDISLLKLVKIE
                     PDDTESLIRMIILCTSDLNSNIARYLISFLSDGRLKEFLELFVENFDFLNIIEGKTSE
                     INF"
     gene            8656..10248
                     /locus_tag="VQU32_001057"
     CDS             8656..10248
                     /locus_tag="VQU32_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2472524.1"
                     /translation="MRLIFRIIRKDIILYSILTFLLSIAMVSEAFLMQFIVDSIKFGE
                     KKYVLISIFIVIFLILQTLLYYYQQFLTAVLSKKSAYEYRKLIFKNIQQTSLRLVTGE
                     KNDSILASLTTQIDQIEYNYFYSVYWGGYLLCQLVVAIVMSLYLNPLMSVLTICLSLP
                     NLFVALLFKNSLEQKQEELINETNSSIAVTQDLIEGITDWKIANREPNIFRVFESKTL
                     KLLEKQVKVEKFQYIVVSLNQLFSNVLYFGSWIVGGFLIIRGQLSLGSLIAFSQLLAR
                     ISYPVYASSDLLSKYISGKKIVEILSKEFIFSDESRNYPDEFNVISFEHYFTNIDEKD
                     MPFDKEFYKNKKYLLKGKSGSGKTTILKSILREYDDYSGIVKINGVDVRDIKESVLFD
                     NIAYVSQKPHLFSASLRDNLTLFSEDFTDNEIVEVLKFVELSKWANKDCLDMMLSNDD
                     IKLSGGEVKRVSLARALLMNKNILLLDEFSAGIDYETLLKIEKKLIDLDKMIIYVTHV
                     DIDRSGKQFDDVIDLDCLFISS"
     gene            10440..11343
                     /locus_tag="VQU32_001058"
                     /pseudo
     CDS             10440..11343
                     /locus_tag="VQU32_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091364.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11361..11498
                     /locus_tag="VQU32_001059"
                     /pseudo
     CDS             11361..11498
                     /locus_tag="VQU32_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
     gene            11526..11867
                     /locus_tag="VQU32_001060"
     CDS             11526..11867
                     /locus_tag="VQU32_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472525.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLKNHI
                     LPLWIGMVSFF"
     gene            11840..12151
                     /locus_tag="VQU32_001061"
                     /pseudo
     CDS             11840..12151
                     /locus_tag="VQU32_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            12279..12866
                     /locus_tag="VQU32_001062"
     CDS             12279..12866
                     /locus_tag="VQU32_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HET2472526.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            13256..14863
                     /locus_tag="VQU32_001063"
     CDS             13256..14863
                     /locus_tag="VQU32_001063"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET2472527.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(15422..17053)
                     /locus_tag="VQU32_001064"
     CDS             complement(15422..17053)
                     /locus_tag="VQU32_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HET2472528.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGITITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            17346..22325
                     /locus_tag="VQU32_001065"
     CDS             17346..22325
                     /locus_tag="VQU32_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HET2472529.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKPRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQK
                     TGRQTLTLRYQGHEVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEELATAKQSLKDLVALLKEDKPAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESA
                     EKPQPAKPAPQNKVLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRV
                     IIQGRDGLLRHVFEVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANEALIAVLASLGLASLALTLRRKREDKD"
     gene            22415..23611
                     /locus_tag="VQU32_001066"
     CDS             22415..23611
                     /locus_tag="VQU32_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET2472530.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            23747..24274
                     /locus_tag="VQU32_001067"
     CDS             23747..24274
                     /locus_tag="VQU32_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET2472531.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            24351..24707
                     /gene="glnR"
                     /locus_tag="VQU32_001068"
     CDS             24351..24707
                     /gene="glnR"
                     /locus_tag="VQU32_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HET2472532.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            24744..26090
                     /gene="glnA"
                     /locus_tag="VQU32_001069"
     CDS             24744..26090
                     /gene="glnA"
                     /locus_tag="VQU32_001069"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET2472533.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(26186..26305)
                     /locus_tag="VQU32_001070"
     CDS             complement(26186..26305)
                     /locus_tag="VQU32_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HET2472534.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            26793..27275
                     /locus_tag="VQU32_001071"
                     /pseudo
     CDS             26793..27275
                     /locus_tag="VQU32_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         8594 a         4569 c         5792 g         8320 t
ORIGIN      
        1 aaccatacct acggcaaggc gaaactgacg tggtttgaag agattttcga agagtataaa
       61 atgatgggga aggctgggca actagtcttt ttcgatgttt atagattagt taggcaagta
      121 agctaggata ggaatctttt tggtgctaag attttaagag aagagactgc aagaagggct
      181 tgatttatga taaaatagaa gtctaagaag cgaatgaaga gagtaagatg aaagaagcta
      241 taattgagtg gaaggatttc tctttccggt atgaaacaca acaagaaccg accttgcaag
      301 ggatagactt gaccatttac aagggagaga aagtcttaat tgttggacca tctggatcag
      361 gtaaatctac cttgggtcag tgtttgaatg ggattattcc caatatttac aagggtcaga
      421 catatggaga atttttgata aagggtcaaa cagcctttga tatgagcatc tatgataagt
      481 ctcatctggt tagcacagtt ttgcaggata cagatgggca gtttattggc ttgtctgtgg
      541 cagaagattt ggcgtttgct ctggaaaatg atgtgacagc cctagatgag atgaaaggtc
      601 gtgtttataa atgggctgaa aagctggacc ttcttccttt actggatcag cgtcctcagg
      661 atttgtcagg tggacaaaag cagcgagtca gtctggctgg tgtcttgatt gatgaaagtc
      721 cgattctctt gtttgatgag ccactcgcca atctagatcc caagtcaggt caggatatta
      781 tcgaattgat tgaccagatt cataaggaag aggggacgac gactcttatt atcgagcacc
      841 gtttggagga tgttctgcat cgccctgtgg atcggattgt cttgataaac gatggtcgta
      901 tcctttttaa tgggagccct gaccagttgc ttgcgactga tttattgact caaaatggaa
      961 ttcgagaacc cctttatcta accactctcc gtcaattagg tgtggactta gtcaaggaag
     1021 aacagttagc gaatctggat aacatgtcta tctcaaaagg tcaggttcag ctgcagaatg
     1081 aactggtaaa agaaactcca gaattgcagt cactctttaa attagaggac gtgtctttct
     1141 cttatgatga tagaccgatt ttaaaatccc tacatttaga tattaaaaag ggtgaaaaga
     1201 ttgctattgt cggaaaaaat ggagcaggga aatcaactct agccaaggct ataagtagct
     1261 ttattcagac ggaaggacgc tatctttggg aaaaacagga tataaaaggc gattctgttg
     1321 cagagcgggc ggaacgagta ggctatgtgc tacaaaatcc taatcaaatg atttcaacca
     1381 atatgatttt tgatgaggtg gctctagggc tccgtttgcg aggtgtggat gagaaggaaa
     1441 ttgaaacgag agtctatgaa accttgaaaa tctgtggact ttatgaattc cgtaattggc
     1501 ctatttctgc cctgtcattt ggtcagaaaa aacgtgtcac cattgcttca attttggtct
     1561 taggagctga aattattctc ctagatgaac cgactgcagg tcaagatcag aagaactata
     1621 ctgagattat ggaatttctc gaagagttac atcgaaaagg gcataccatt gtcatgatta
     1681 cccatgatat gcaattgatg ctggattatt cagaccgagt ccttgtcatg gtggatggag
     1741 aattgattgc cgatactgtt ccagccagtc tgttgagcga tcctgagctg ttagtaaaag
     1801 ccaatctaaa agaaacctcc atctttaact tggctaagaa actagatgtg gatccactgg
     1861 atttaacggc attttacaaa gaaaggagag agggatgcaa gctaaattaa tcggttacca
     1921 gcatagagat actgtgattc atcgcttgtc aggagctggg aaacttctct ttttcattct
     1981 ggtatcattg gcggccatga ttagctatga taccagaatg ctcttgctga ttgccatctt
     2041 ttcagtcttt ctcctctatt tatcagaaat tcgctttaaa gatgtttcct ttgtagccgt
     2101 ttttgcgacg gtatttgccg ttttaaacgt tttgatggtc tatctctttt ctcccgagta
     2161 tggggttgga ctttatggag agagaagtgt gatttggcag ggaatcggtg tctacactct
     2221 aaccagccag gagctctttt atctgctaaa tttggccatt aagtatcttt gcaccattcc
     2281 tctggctatt atctttttga tgacaaccca tcctagtcag tttgcttcca gtttaaatca
     2341 aattggtgtg ccctataaga ttgcttattc tgtcagcctg accttgcgct atattccaga
     2401 tttgcaggaa gaattcttta ctatcaagat gtctcaggag gcgcgtggga tggaattatc
     2461 caagaaagct tctcttatgc aacgaatcaa aggcaatctg ctcattatta cgcccttgat
     2521 ttttagctcg ctagaacgca ttgataccat tgcgactgcc atggagcttc gccgctttgg
     2581 gaaagaaaaa aaacgcacat ggtatagtta tcaggccttg aaaaaaggag actatattac
     2641 cttgctcttg gcaaccttgt ttttagtagc tagtttacta cttagcttgc agaatcaggg
     2701 acgattttac aacccttgga aatagcttga aaaaattgaa aaaatcaagt cgtttctatt
     2761 gacaatgatt ctgaaagtgt tatactaaga aagtagtttc gctgatttac ttcaaacctg
     2821 ttgtgaggta agttaacgat gccttaacca cgctgtttgc tgagcttgac tccgggcagt
     2881 gtggctattt ttttgcaatg gtgaaaggaa gcaagtcatg acaaatcaca ttgtattatt
     2941 tgaacctcaa attccacaaa atacgggcaa tatcgcgcgt acttgcgctg cgaccaattc
     3001 tcccctccac atcatcaagc ctatgggctt tcctattgat gaccgcaaga tgaagcgggc
     3061 tggattggat tactgggata agctagagat ttatttttac gagagtttgg aagatttcat
     3121 atctcagatg aagggaaaac tctatctgat ttcgaaattc gcggaaaaag tctattctga
     3181 ggtggattta tcgactgacg aggaccatta ttttctcttt ggacgtgaag acaagggctt
     3241 acctgaagat tttatgcgag aacatcctga gaaggctctc cgtattccca tgaatgatga
     3301 acatgtccgc agtctcaatg tgtctaatac cgtctgcatg attgtctatg aggctcttcg
     3361 ccagcagaac tttgcaggtc ttgaactcgt ccatacctat gaagtggata aattgaaata
     3421 aaaaatgaaa atgaacaaaa tgcttgcgct tgcaagcgtt ttttgttatg ataaaagagt
     3481 cttcagggca gggtgtgatt cccgaccggc ggtgacttta actaggaaat gatcttttcc
     3541 ttttatactt tgttgacaag ctttgcctaa ccagaagtta tgcctacagc ttgtcgccta
     3601 gtctaaaaga aaaatctcta tttccttctc tttaaagaag tccgcgagcg caagctgatg
     3661 tggtgagatt ccacaaccga cagtatagtc tggatgggag aagacgaaag aatagctttg
     3721 tctgttttga tagatttata gccggattgt aaccacttgc tttgtttttc tttaatagag
     3781 taggagggaa cttttgggat ataaaaagtg agaatagata gaggaatcct ttccaacttc
     3841 ttctgatttt atagaaaatt ggaggaacct gttatgacaa acacacgtcg actttcgacc
     3901 attgcaattt tatcagccat ctcatttgtg ctgatgtact ttgactttcc gcttttacca
     3961 gcggcatctt tcctcaagat cgaatttagt atcttgccag tccttgtggg tctggtggtc
     4021 atggatttgc ctgctgctct aggagtgctc ttgcttcgct cactcttgaa attgcttctt
     4081 aacagccaag gagtgaatac ttacattggt ttgccgatga atatcgtagc tttgggagtt
     4141 tttgtcatcg tatttgcttt gatttggaaa aaggaacgga caacccttcg tttcctacta
     4201 ggctctctag ctgggactat tggtttaacc gtggctatgt tagttctcaa ctatgtttat
     4261 gctgttcctt tgtacgctaa gtttgctaac tttgatattg gaaaaatttt gggactttcc
     4321 aactacctaa tgaccatggt attacctttt aacttgattg aaggtgtaat cttttccgtt
     4381 tcattctggt tgttgtacat tctcttgaaa ccaaccttaa aacattatga aagataaaca
     4441 aacattttta atgaagggca gttttgccct tttacttttc gttattcttg gctatatggt
     4501 caaattttac cctgaaatgt tggtcaattt tgaccaatcg attcagactg ccattcgagg
     4561 agacttacca gattatttga ctattctttt tcgatccctc acacgtctga ttgatattcc
     4621 agtgattatc acttgggttg tcattatagc ttttgtcttt tatcgtaagc gatggaagat
     4681 agaaagtttc ttcatgctgg gaaatctgac tttggcaggt cttttaatcg tgacctttaa
     4741 aaatatctac cagcgcccac gaccagctat cttacatctg gtggaggaga agggattttc
     4801 cttcccaagt gggcattctc tggctgtaac gctgatggta gggactttga ttgttattct
     4861 tagtcagcgg attaaagatc aggtctggag aaaaatcgta caaatcgtcc ttggcctcta
     4921 cctagtcagt gtgctggtat caagagtcta tctgggagtt cattacccat cagatgttct
     4981 tgccagtctc tgtgtgggct tgggagccct gtttatcgag tttccctttt atgataagct
     5041 ccgcttccaa tggcgattta aaggcaaaca gaaatgatgg aaaagataaa aaattgtaca
     5101 aatacaaata gaaccatatg aaatatatac ttgaaacatg ttttggagtt tgatatattt
     5161 taataaataa agcaaggagg aagaaagtgt gggtatgtta ttagcgacac gcttaaaaaa
     5221 taaaagaaag gaattaaaat tatctcaaaa agaattagct gaggggattt gtgagcaagg
     5281 gcagattagc cgtatggagc aaggtaaata ctcacctggg tcagaattac tctttcaact
     5341 atctaagcga ttgaaggtga gcatggatta tttctttgaa gatactgagg tttctagttt
     5401 agaaaatatt gataagttta aagaattatc taagaaattt ttggatgaga gggaatatga
     5461 atctctccat tacatctatg aactggaaaa atctaaacgt gctcgtctat ctgctaaaga
     5521 ccagatttat ttggattgga ttgatacatt agttcaattc tgttatttaa atcatcaaac
     5581 aggggcagtt gcgaaactgg aaaaaatact gttatctctg aaaaaatctg atttttacta
     5641 tctacaactg ggaaatacct tggctaattt ttattttgat atggggcagg aagacacata
     5701 tcaggaactc tatcaacttt taaaagagaa cctccagtca ctatctgttc atcatttgga
     5761 acagttagaa ttgatgatta aattccgata taactattgt agatatttgt ggttgaatga
     5821 acgaacatct gaagctatag aagaaattac agaaacgata gctatttgta aaagatataa
     5881 tagtagttat cgattagcag atttgtattg tttattggga agtataagcg agggctttgc
     5941 tgaaagggat aggatactag gtttttatca agtatcttac cagctttaca agcacgaaga
     6001 gaaccagaga atgatgctag aaattgagaa ggaaatgaaa ttattggaaa gtgaagatta
     6061 agcaaaaaga tttgtattct tagataactt taatacatat tataaaaaaa ataaaaaaat
     6121 atcataaaaa ttattgacaa accaaaattg atgtgttaga atatcaccaa tagttaagga
     6181 ggtttagaag atgaaaagat ctcaagttgt caaacgatgt atattaattg ctttattgct
     6241 tctatttcta cttaatgtgt taagtggttc tttatctgta acaactagag gagttgatcc
     6301 aagaccagca tatggagttt cgctttaaag ttgtgcatag ttcttctttt atagacggag
     6361 agtttttctc aatagtaagt gattcaaatt ttgatggatg attttccatt aaattaagtg
     6421 attgcgaaaa ctttttgaaa tacattctct ttggatattg aaagtttgtt ttggattttt
     6481 ttatgaagct tatcttagct gttagattgg ggttttatgg gatttgtcct caccgggaaa
     6541 aaatcttaaa tctttgaaga aagaggtttt tatcttatgg ttagagatac atcagaagaa
     6601 attgaagtta ttgacttgtc ttctatgacc gtttcctact catctgttat gctaggaaac
     6661 tcttggatga cgtggaattg atttattaag atagattatt tggtttttac caaaaatctg
     6721 tctttttaat ttttaaagga ctaatggtga tatgaaacta tatcaaataa atgatgggat
     6781 acaaattttt gaaagtagta atcttgaaaa aatagtaatt agaaattata ggaatggtaa
     6841 gaaaataact atcgatcacc aaccattatt aaaaaaatta ctactattcc ttgaagttcc
     6901 taaacgtgaa gaagaaattc tattatatct tccagaatta tcaaataaaa cattacgaag
     6961 tgttttgacg aattttatga gtttaaatat aattcattgt caagatacat taataattca
     7021 caaaaaaata aacatattga taataggttt agggactacg ggtggatatc tggcagatgg
     7081 gttatgtcgt agtaatatta attcgaagct atttctttta gataaggatc gtgtagatga
     7141 gactaatgta ggtagacaga tttatttaag agatgatatt ggtcaatata aagttgatgt
     7201 atttaaacaa aagtttagtg ataaagaaat aaaggtattc aaaattttta ttaatagtaa
     7261 gaaagatttg gaaaagattt gcaaaaggga gggtatcgat ttggttatac aatgtggtga
     7321 ctccccttca cctcgagaat taggaaaaat tgtgaataca gtatgtgata ctctccagat
     7381 accttatata gttaacactg gctacatgag taatgtagtt cctttgccag aattttatta
     7441 tccaaataat agatacaatt ttaattataa gcatcaattt tcagatgaaa agttactgtt
     7501 ctcacaagtt ttgtctaaag ctgattatat ggtggcaatt caatcatcat ttgtaatgtt
     7561 aatgcaaata aaaaatttta tatttaatga agaacctatt tattatggtt ataggggata
     7621 ctataattct gtaacattag catgggaggt ggaaaaaatt gactaaatat ttagaattgg
     7681 attattctca ttcatatatt ttggaaggtt ttagaaaaaa tgatgcgttt attgaaaatc
     7741 acaaagaatg ccttgatatg ttatcagata agatttggcg agataataaa tatatgaata
     7801 cagaaaaaaa catatcatca ccgacacgaa caatcttatc acgctatact tgtaatattt
     7861 tatcgtcttt gtttattgat attagcaaaa atagcataga aaaattgata gtgacatgtg
     7921 aaaatagcga tcttaattgg atttataatt attataaatc ggtttacaat ggagtatttt
     7981 gtgaattgcg acaacttata caacagtact gtaaggtgaa ggataagtat gaaaagtggc
     8041 tttttataaa agctattata aatcaaggta ttcgtcaaaa tgagaaagaa gttgttgaat
     8101 tctttttaaa acaattgaag gataataatc aaggtatttt tgattacatc aatagtttta
     8161 gtttttatct attaaagttt tattcaaaag acaaatctcg cattttttta gaagagcagc
     8221 tagatataga tgattttttg ggatcagata aagaagatta tgctaggtct ttagttttta
     8281 agaattatgc ttctctattg cctgatactt ctgaattaaa aagaaatatt atggaaaaat
     8341 gtttaagtca aacacctcaa gatacagatt tatggaaaga atggtttgaa acttatgcca
     8401 gtcagaaaga gattagacaa aaagcaacgg aaatatttaa gcatggttat tcagatattt
     8461 cgttactaaa attagttaaa attgagcctg atgacactga gagtttaatc agaatgatta
     8521 tcttatgtac cagtgaccta aatagtaata ttgcaaggta tttgatttca tttttgagcg
     8581 atgggcgatt aaaagaattt ttggagttat ttgttgaaaa ttttgacttt ttgaatatta
     8641 ttgagggaaa aactagtgag attaattttt agaataatta gaaaagatat tatattatat
     8701 tctatattaa catttttgct atcaatagcg atggtaagcg aagcattttt gatgcaattt
     8761 attgttgatt caattaaatt tggtgaaaag aagtatgttt tgatttctat ttttattgta
     8821 atttttttga ttcttcagac tttattgtat tactatcaac aatttcttac agctgtatta
     8881 agtaaaaaat ctgcatatga gtaccgtaaa ttaattttta aaaatattca gcagacttct
     8941 cttaggcttg ttacaggtga aaaaaatgat agtattctag cttctttgac aacgcaaatc
     9001 gatcaaatag aatataatta tttttattct gtttattggg gtggatattt gctttgccaa
     9061 ttagtggtag ctatagttat gtcgctttat ctaaatccat tgatgtctgt attaaccatt
     9121 tgcttaagtc tccctaattt atttgtagca cttcttttta aaaactcctt ggaacaaaag
     9181 caagaagagc taattaatga gacaaattcc tcaatagcag ttactcaaga tttaattgag
     9241 ggaattactg attggaaaat tgcaaataga gaacctaata tatttagagt gtttgaaagc
     9301 aaaacattga agcttttaga gaagcaagtg aaggtagaaa aatttcaata cattgttgtt
     9361 agtttaaatc aattgttttc aaatgtttta tattttggta gttggatagt aggtggattc
     9421 ttgattatac gggggcaact ttcattaggg agtttgatag cgttctctca attattagca
     9481 agaatttctt accctgttta cgcttcttcg gatttattgt caaaatatat tagcggtaaa
     9541 aaaattgtag aaattctttc taaagaattt atattttcag acgaaagcag aaattatcca
     9601 gatgagttta atgttatttc atttgaacat tattttacaa acattgacga aaaggatatg
     9661 ccttttgata aagaatttta caaaaataaa aaatatttgt tgaaagggaa aagtggtagt
     9721 gggaaaacaa caattttaaa atcgatttta cgagagtatg atgattatag tggaattgta
     9781 aaaattaatg gggtagatgt tagggatata aaagagagtg ttctttttga caatatagct
     9841 tatgtttccc aaaaacctca cttgttcagc gcaagtctta gagacaacct gacattattt
     9901 tctgaagact tcactgataa tgagatagtt gaggttttaa aatttgttga attatctaaa
     9961 tgggctaata aagattgctt ggatatgatg ctttcaaatg atgatataaa attatcagga
    10021 ggggaggtca aaagggtttc tttagctcga gccttattga tgaataagaa tattctgtta
    10081 ttggatgagt tctctgctgg gattgattat gaaacattgt taaaaataga gaagaaactc
    10141 attgatttgg ataagatgat tatttacgtg acgcatgtgg atatagatag atccggcaaa
    10201 cagtttgatg atgtcattga tttggattgt ctttttatat cctcgtgaca tgagagttga
    10261 gtcagttgaa ttattttgtc actctgttta gttcttgttg gtagtgtcag gtaatctttg
    10321 ttaatttttc aggaagtcta ttgaaatatc ctcttggtta tatattacga ggagtaatta
    10381 atggatattc tcgagaattc aatacaacaa tttgattcta aagttaagta tgtttcttta
    10441 tttgcggaat caatctttta tcagatacca tgagctattt tgcaggtgct atgatttctc
    10501 ctttgtatgt gaggattatc ggagatgata tgacagatgc tatgggggtt agacaggcaa
    10561 ctgctggtgt agttaatatt ctcagcaacc ttataggggg agttttgatt gggggtcatc
    10621 agtcttcaga tgtttgtagg tttgaatgct ttgacctttc tttttgcact gttgggtgtg
    10681 gtgtttattc gtacttcttt gagagatatt gaggaacaga tggaggttag taaaagatta
    10741 gccgcaaaat cttttggaga gcatttcctc cattcaatga agatattgtc aagttttcca
    10801 gccatcatca aattaatatt cattgcagct atgaaccaag tgattttaag cattattatc
    10861 cctttaacga ctttgatgtt aatccatcat ccatttatct tctttgacac tgggaaatcc
    10921 attgcagcat tgtcagtggt tattttttca ggaacgattg tgggaagttt tttgagtgga
    10981 ggtatcctaa aaaatattcc cataaaaatg attgtctact ttgaagaaat tatgcatctc
    11041 tgtattctct tttcttggtt gaccaatcat ttcctccttt tattggtagt tgtcttctta
    11101 tgcgcagttg cagtaggaat gatggggcct cggattttca accttgtatt ctcgatggtt
    11161 ccagaagaag tcatgggaac catccagtca gtgcttggag tattcaatgt tgttgtacca
    11221 ggaattctta gcatgctcat cgttgcctta gcgagtgcta caaacctaaa aatcacagtg
    11281 cttccacttt ttatttttgt attgactgcc ttagcaattg tgaaagcaat gaaaatagag
    11341 taatagtagt atgaactact gtctcttttt gggtgggagt tcattaccca tcagacgtcc
    11401 ttgccagtct ctgtgtgggc ttgggagttt tgtttatcga gtttcccttc tatgacaagc
    11461 tccgcttcca atggcgattt aaaggcaaac agaagtgagt atcgaattcc cttgaggaga
    11521 aagaaatgaa agtcaatata gcagatcttc atccgactca actatattta tcagaaaaga
    11581 agttgcaaga tattcagatg ctttaccagt cggcagaaac aatccaagtc gatccaatca
    11641 gtattcttgc ctttggagat tgtttgctga ttacagatgg gcatcacagg gcttatcagg
    11701 ctttattggc aggtcgggat acgatttctg ctgagtggga tagagatggt ggtgatgaac
    11761 tatatcatct ctatgcgcaa gcttgcgagg aaagaaagat ttactctgtt tttgatttaa
    11821 aaaatcatat cttacctctt tggatcggca tggtgagctt tttttgattg aagtcaagga
    11881 gaagggagaa tggtttccaa ttggggatat tacactatct caggataatc tccccattgt
    11941 gattgggaat tccgcttacc aacatcgagg acttggaaaa aagattctaa gtgctttgat
    12001 tgaattggct cgagtaaaag gatggaaaga attgagagtc aaggaaatct acacctacaa
    12061 tcatgcttct aggaggtgtt tcaagtcgct tggatttgtg gaaaatggag caacagaaaa
    12121 aggaaggagt tttatattgg aattagtcta atcctgccac tttctccccc ttaacctttg
    12181 actattccgc aaaattatcg taaaataaag agtaaatgat aaaatgaggt cagagtctgt
    12241 tcgctctgac gatagtagta taaatgagga gaaacgcttt ggaattagaa gtatttgctg
    12301 ggcaagaaaa aagtgaacta tctatgattg aggtagcgcg tgctatatta gaacttcgtg
    12361 gtcgcgatca tgagatgcat tttagcgatc ttgtaaacga aattcaaaac taccttggaa
    12421 catcaaacag cgatatccgc gaagctttgc ctctgttcta cacagagttg aactttgacg
    12481 gtagcttcat ctcacttggg gacaacaaat ggggtcttcg ttcatggtat ggtgtggacg
    12541 aaatcgacga agaaatcatc gctcttgaag aaaatgacga cgatgaagta gcaccaaaag
    12601 ctaagaaaaa acgtgtcaat gcctttatgg atggtgattc agatgccatt gactacaatg
    12661 cagatgatcc agaagacgaa gatgcatacg aagcagatcc agctctttca tacgatgatg
    12721 aaaatccaga tgatgaaaaa aatgaagtgg aagcttatga tgcagaaatc aacgaaatcg
    12781 ctccagatga cttgggagaa gatgtggatc tcaacgaaga cgacgacgag ttttcagatg
    12841 acgacgctga aaccagcgag gaataagagt tagctattga caattatcct atttttaggt
    12901 atcatattgt ttgggcacct cttttagagg tcggggctcc ctagttctta gggagctatt
    12961 tttgtttttt caagaagtta tcttcttgta ttttatactc aatgaaaatc aaagagcaag
    13021 ctaggaaact ggccgtaggc tgctcaaaac actgttttga ggttgtagat aagactgaca
    13081 aagtcaggaa cacatatcta cggcaaggcg acgttgacgc ggtttgaaga gattttcgaa
    13141 gagtattagt tgtgaatctg gtgcagtcgt cccagattat tcttattagt agggtcttgt
    13201 tttctatatc ccctcgtagt taacaagacc ttgagcattt tagaaagagg aatctatgtc
    13261 tacgaaatat atttttgtaa ctggtggtgt ggtatcgtct attgggaaag ggattgtggc
    13321 agcgagtcta ggccgtctct tgaaaaatcg tggtctcaaa gtaaccattc aaaagtttga
    13381 cccttatatc aatattgatc cgggaaccat gagtccttac cagcacgggg aagtttttgt
    13441 gacagatgac ggagctgaga cagatttgga cttgggtcac tatgaacgtt tcatcgatat
    13501 caatctcaac aaatattcca acgtgacaac tgggaaaatt tacagtgaag ttcttcgtaa
    13561 agaacgccgt ggagaatacc ttggggcaac tgttcaagtc attcctcata tcacagatgc
    13621 tttgaaagaa aaaatcaagc gtgccgctct aacgaccgac tctgatgtca ttatcacaga
    13681 ggttggtgga acagtaggag atatcgagtc cttgccattc ctagaggctc ttcgtcagat
    13741 gaaggcagat gtgggtgcgg ataatgtcat gtatatccat acaaccttgc ttccttacct
    13801 caaggctgct ggtgaaatga aaaccaaacc aacccaacac tctgtcaaag aattgcgtgg
    13861 cttgggaatc caaccaaata tgttggttat tcgtacagaa gagccagctg gtcaaggaat
    13921 taaaaataaa ctggcccagt tctgtgatgt ggcaccagaa gccgttatcg aatcgttgga
    13981 tgttgaacac ctttaccaaa ttccactgaa cttgcaggca caagggatgg accaaattgt
    14041 ttgtgatcat ttgaaattag acgcaccagc agcggatatg acagaatggt cagccatggt
    14101 ggacaaggtc atgaacctca agaaacaagt taagatttcc cttgttggta agtatgtgga
    14161 gttgcaagat gcctatatct cagtggtcga agccttgaaa cactctggct atgtcaatga
    14221 tgtagaagtt aaaatcaatt gggtcaatgc caatgatgtg acagcagaga atgtagcaga
    14281 actcttgtct gatgcggacg ggatcatcgt accaggtggt tttggtcaac gtggtacaga
    14341 agggaaaatc caagccatcc gctatgcgcg tgaaaatgat gttccaatgt tgggagtctg
    14401 cttgggaatg cagttgacat gtatcgagtt tgctcgtcac gttttaggtc ttgaaggtgc
    14461 caattctgca gagcttgcac cagaaacaaa ataccctatc attgatatca tgcgtgatca
    14521 gattgatatt gaggatatgg gtggaaccct tcgtttggga ctttatccgt ctaagttgaa
    14581 acgtggctct aaggctgctg ctgcttatca caatcaagaa gtggtgcaac gccgtcaccg
    14641 tcaccgttat gagtttaata atgccttccg tgagcagttt gaggcagcag gttttgtctt
    14701 ttcaggagtt tctccagaca atcgtttggt agaaatcgtg gaaattcctg aaaataaatt
    14761 ctttgtagct tgtcagtatc accctgaact gtcaagccgt ccaaaccgac cagaagaact
    14821 ctacactgcc tttgttactg cagcggttga gaacagcaat tagcaaaatc agaacctttg
    14881 agaaaaatct cagaggtttt ttgcatacga tgatattgca atatatctga ggtagggggc
    14941 ctctgtatgt acctgctacc gttgaaatca atagcgactc cctcttgccc tgtgctagtg
    15001 aatggattta tcagtatatt gaaatgaaat aaaatttgaa caaattaatt cggaaagcca
    15061 aatcaatttc tagcaaagtt ttaggaactg gattgtatag tgaattgaaa taagatgtga
    15121 acatctctat cagaaaagtc aaattaattt atagaaatat tttagcagtc aagatgtact
    15181 gttatagatt caatacatta tactttttta atttaatcca ctatagtaaa atgaaataag
    15241 aacaggacaa atcgatcagg acagtcaaat cgatttctaa caatgtttta gaaatagagg
    15301 tgtactattc tagtttcaat atactatata tctgcagaat actctttcag tttcattcag
    15361 cctagtcttg cttaggaaaa agtaaaaagc catccaatta tgatggatag cttcttggtt
    15421 cttagatttg ttctgcaaaa accttttcag caaggttcat agcgtggtct gatacacgag
    15481 tgtagtgtga gatgatgtcg ataaagttga ccccagcttg tgttgaacat tcgcctttgt
    15541 tgaggcgttt gatgtgggtt tttctgagaa cacgttctat cttattgatt gcttcatgac
    15601 gttctacaag actgcgtgct ttttcaatat cattgttttc cacactatcc agagcatctt
    15661 tgataaagtc actagtttgg cggtaaactt cctctaattc tttcaaggcg gcatcagaaa
    15721 attcaacatt tttccgttga agatagtcag tcagattgag tagagcctcc gtgtggtctc
    15781 caatccgttc caaatcacgg gaggaatcaa ggatattggt aagcacttca ctttcttttt
    15841 ggctgagagc ttcgcttgaa agggcaatga gataacgtgt taattgctca tcgatggtgt
    15901 taattgcttc ttcggtttta tgcccttttt cagcaacttt ttcatccaag tcaatgatgt
    15961 acttatagga aaggtcaaag gctttagcag cgtagtttcc taagtgcaag agctctttct
    16021 tagcatttcc tagagcgata gatggggcct gtttgatgaa atgttcatca agatataagg
    16081 gttcgtattt gacaacctcg tcctctccag gaataatctt ggttacaaag taagccagag
    16141 ctccgataaa tggaaattgg acaatggtgt tggtaatatt aaaggttccg tgagcaaagg
    16201 cgatggtcat ttccggtgct agatttagcg tagcttcaaa ccaatgaatc aggacagtaa
    16261 aaggaactag aaaaataacg cagacgactg ttccgataac gttgaaggca acatgagctc
    16321 ctgctacccg tttagctgca atattagccc ctaaagaggc aatgatggct gtaatggtta
    16381 tcccgatatt gtcaccaaat agaactggca aagctccctg taggtcaatt agattgccgg
    16441 cgtagaggtt ttgtaaaatc ccaatggtag ccgaagaagc ttgaattagc aaggtcaagc
    16501 cggtaccgac aaagacaccc aaaacaggat tcttacttag ctcaatcata tagtccttaa
    16561 agacctgtaa atccttgagt ggagccattg cgccgctcat gagattgagg gcaaaaaaga
    16621 taccaccgac accaaagagg atgcgtccga tattattgac tgtccgattt tttgtaaaaa
    16681 agagacagac ggcaccgata aagagcatag gtagggcata gttacctaat ttaaaaccga
    16741 tgagaaagga tgtgacagtt gtcccaatat tagcacccat gacaatcccg atagcctgac
    16801 gtaaggttaa gagaccggca ctgaccaggc cgactgtgat aactgttaca ccagaactag
    16861 actgaattag agcagtcatc ccaataccaa ccagaactcc aaagaaagga ttactagtat
    16921 atttgtcaat gtaaaaacga aggcgatctc cagcagcttg ttgcaaaccg tctcccatgg
    16981 tcttgatgct atataagaat agccccagac cacctaaaaa gtgaaataaa atttcctgcc
    17041 aattaatgga catttctttt tcctccgaaa aataatcgcg gaatatcttc tattctattt
    17101 taaaggataa aagtaaatct aacaagtgtt aatgtcaagt ttgtaaaaga aaattataat
    17161 tgataagtgt attattttta agagttttta accttatttt agatttgtgc aaacagttat
    17221 tgttttttgt ctaaaaatgt tttaggataa aaatgtgatt atcatggttc tagagtttga
    17281 taaaaaacta cagaatataa agcgctttct tgaaaacaac aaaatcaatc ttttaggagg
    17341 agaaaatgaa gaatccattt tttgaaagac gttgtcgtta cagtattcgt aagttatcag
    17401 taggagcctg ctcgctgatg attggtgctg ttttatttgc tggtccagcc ttggctgaag
    17461 aaactgcagt tcctgaaaat agcggagcta atacagagct tgtttcagga gagagtgagc
    17521 attcgaccaa tgaagctgat aagcagaatg aaggggaaca tgctagagaa aacaagctag
    17581 aaaaggcaga aggagtagcg atagcatctg aaactgcttc gccagcaagc aatgaagctg
    17641 caactactga aactgcagaa gcagctagcg cagctaaacc agaggaaaaa gcaagtgagg
    17701 tggttgcaga aacaccatct gcagaagcaa aacctaagtc tgacaaggaa acagaagcaa
    17761 agcccgaagc aactaaccaa ggggatgagt ctaaaccagc agcagaagct aataagactg
    17821 aaaaagaagt ccagccagat gtccctaaaa atacagaaaa aacattaaaa ccaaaggaaa
    17881 tcaaatttaa ttcatgggaa gaattgttaa aatgggaacc aggtgctcgt gaagatgatg
    17941 ctattaaccg cggatctgtt gtcctcgctt cacgtcggac aggtcattta gtcaatgaaa
    18001 aagctagcaa ggaagcaaaa gttcaagcct tatcaaacac caattctaaa gcaaaagacc
    18061 atgcttctgt tggtggagaa gagttcaagg cctatgcttt tgactattgg caatatctag
    18121 attcaatggt cttctgggaa ggtctcgtac caactcctga cgttattgat gcaggtcacc
    18181 gtaacggggt tcctgtatac ggtacactct tcttcaactg gtctaatagt attgcagatc
    18241 aagaaagatt tgctgaagct ttgaagcaag acgcagatgg tagcttccca attgcccgta
    18301 aattggtaga catggccaag tattatggct atgatggcta tttcatcaac caagaaacaa
    18361 ctggagattt ggttaaacct cttggagaaa agatgcgcca gtttatgctc tatagcaagg
    18421 aatatgctgc taaggtaaac catccaatca agtattcttg gtacgatgcc atgacctata
    18481 actatggacg ttaccatcaa gatggtttgg gagaatacaa ctaccaattc atgcaaccag
    18541 aaggagataa ggttccggca gataacttct ttgctaactt taactgggat aaggctaaaa
    18601 atgattacac tattgcaact gccaactgga ttggtcgtaa tccttatgat gtatttgcag
    18661 gtttggaatt gcaacagggt ggttcctaca agacaaaggt taagtggaat gacattttag
    18721 acgaaaatgg gaaattgcgc ctttctcttg gtttatttgc cccagatacc attacaagtt
    18781 taggaaaaac tggtgaagat tatcataaaa atgaagatat cttctttaca ggttatcaag
    18841 gagaccctac tggccaaaaa ccaggtgaca aagattggta tggtattgct aacctagttg
    18901 cggaccgtac gccagcggta ggtaatactt ttactacttc ttttaataca ggtcatggta
    18961 aaaaatggtt cgtagatggt aaggtttcta aggattctga gtggaattat cgttcagtat
    19021 caggtgttct tccaacatgg cgctggtggc agacttcaac aggggaaaaa cttcgtgcag
    19081 aatatgattt tacagatgcc tataatggcg gaaattccct taaattctct ggtgatgtag
    19141 ccggtaagac agatcaggat gtgagacttt attctactaa gttagaagta actgagaaga
    19201 ccaaacttcg tgttgcccac aagggaggaa aaggttctaa agtttatatg gcattctcta
    19261 caactccaga ctacaaattc gatgatgcag atgcatggaa agagctaacc ctttctgaca
    19321 actggacaaa tgaagaattt gatctcagct cactagcagg taaaaccatc tatgcagtca
    19381 aactattttt cgagcatgaa ggtgctgtaa aagattatca gttcaacctc ggacaattaa
    19441 ctatctcgga caatcaccaa gagccacaat cgccgacaag cttttctgta gtgaaacaat
    19501 ctcttaaaaa tgcccaagaa gcggaagcag ttgtgcaatt taaaggcaac aaggatgcag
    19561 atttctatga agtttatgaa aaagatggag acagctggaa attactaact ggctcatctt
    19621 ctacaactat ttatctacca aaagttagcc gctcagcaag tgctcagggt acaactcaag
    19681 aactgaaggt tgtagcagtc ggtaaaaatg gagttcgttc agaagctgca accacaacct
    19741 ttgattgggg tatgactgta aaagatacca gcctaccaaa accactagct gaaaatatcg
    19801 ttccaggtgc aacagttatt gatagtactt tccctaagac tgaaggtgga gaaggtattg
    19861 aaggtatgtt gaacggtacc attactagct tgtcagataa atggtcttca gctcagttga
    19921 gtggtagtgt ggatattcgt ttgaccaagc cacgtaccgt tgttagatgg gtcatggatc
    19981 atgcaggagc tggtggtgag tctgttaacg atggcttgat gaacactaaa gactttgacc
    20041 tttattataa agatgcagat ggtgagtgga agctagctaa ggaagtccgt ggtaacaaag
    20101 cacacgtgac agatatcact cttgataaac caatcactgc tcaagactgg cgcttgaatg
    20161 ttgtcacttc tgacaatgga actccatgga aggctattcg tatctataac tggaaaatgt
    20221 atgaaaagct tgatactgag agtgtcaata ttccgatggc caaggctgca gcccgttctc
    20281 taggcaataa caaggtacaa gttggctttg cagatgtacc ggctggagca actattaccg
    20341 tttatgataa tccaaattct caaactccgc tcgcaacctt gaagagcgaa gttggaggag
    20401 acctagcaag tgcaccattg gatttgacaa atcaatctgg tcttctttat tatcgtaccc
    20461 agttgccagg caaggaaatt agtaatgtcc tagcagtttc cgttccaaaa gatgacagaa
    20521 gaatcaagtc agtcagccta gaaacaggac ctaagaaaac aagctacgcc gaaggggagg
    20581 atttggacct tagaggtggt gttcttcgag ttcagtatga aggaggaact gaggacgaac
    20641 tcattcgcct aactcacgca ggtgtatcag tatcaggttt tgatacgcat cataagggag
    20701 aacagaatct tactctccaa tatttgggac aaccagtaaa tgctaatttg tcagtgactg
    20761 tcactggcca agatgaagca agtccgaaaa ctattttggg aattgaagta agtcaggaac
    20821 cgaaaaaaga ttacctagtt ggtgatagct tagacttgtc tgaaggacgc tttgcagtgg
    20881 cttatagcaa tgacaccatg gaagaacatt cctttactga tgagggagtt gaaatttctg
    20941 gttacgatgc tcaaaagact ggtcgtcaaa ccttgacgct tcgttaccaa ggtcatgaag
    21001 tcaactttga tgttttggta tctccaaaag cagcattgaa cgatgagtac ctcaaacaaa
    21061 aattagcaga ggttgaagct gctaagaaca aggtggtcta taactttgct tcaccagaag
    21121 taaaagaagc tttcttgaaa gcaattgaag cggccgaaca agtgttgaaa gaccatgaaa
    21181 ctagcaccca agatcaagtc aatgaccgac ttaataaatt gacagaagct cataaagctc
    21241 tgaatggtca agagaaattt aaggaagaaa agacagagct tgatcgttta acaggtgagg
    21301 ttcaagaact cttggctgcc aaaccaaacc atccttcagg ttctgcccta gctccgcttc
    21361 ttgagaaaaa caaggccttg gttgaaaaag tagatttgag tccagaagag cttgcaacag
    21421 cgaaacagag tctaaaagat ctggttgctt tattgaaaga agacaagcca gcagtctttt
    21481 ctgatagtaa aacaggtgtt gaagtacact tctcaaataa agagaagact gtcatcaagg
    21541 gtttgaaagt agagcgtgtt caagcaagtg ctgaagagaa gaaatacttt gctggagaag
    21601 atgctcatgt ctttgaaata gaaggtttgg atgaaaaagg tcaagatgtt gatctctctt
    21661 acgcttctat tgtgaaaatc ccaattgaaa aagataagaa agttaagaaa gtatttttct
    21721 tacctgaagg caaagaggca gtagaattgg cttttgaaca aacggatagt catgttatct
    21781 ttacagcacc acactttact cattatgcct ttgtttatga atctgctgaa aaaccacaac
    21841 ctgctaaacc agcaccacaa aacaaagtcc ttccaaaacc tacttatcaa ccggcttctg
    21901 atcaacaaaa ggctcctaaa ttggaagttc aagaggaaaa ggttgccttt catcgtcaag
    21961 agcatgaaaa tgctgagatg ctagttgggg aacaacgagt catcatacag ggacgagatg
    22021 gactgttaag acatgtcttt gaagttgatg aaaacggtca gcgtcgtctt cgttcaacag
    22081 aagtcatcca agaagcgatt ccagaaattg ttgaaattgg aacaaaagta aaaacagtac
    22141 cagcagtagt agctacacag gaaaaaccag ctcaaaatac agcagttaaa tcagaagaag
    22201 caagcaaaca attgccaaat acaggaacag ctgatgctaa tgaagcccta atagcagtct
    22261 tagccagcct tggtcttgct agtttagcct tgaccttgag acggaaaaga gaagataaag
    22321 attaaatatc gaaaaatctt gtgaaatctt tccttatatt tccaaagtgt gatataatag
    22381 tttcgaataa aataaataaa ggagtttttg taacatggca aaacttactg ttaaagacgt
    22441 tgacttgaaa ggtaaaaaag tcctcgttcg tgttgacttc aacgtaccat tgaaagatgg
    22501 cgtaatcact aacgataacc gtatcacagc agctcttcca actattaagt acatcatcga
    22561 acaaggtgga cgtgcaattc ttttctctca ccttggacgt gtgaaagaag aagctgataa
    22621 agctggcaaa tcacttgctc ctgtagcagc tgacttggca gcaaaacttg gtcaagatgt
    22681 tgttttccca ggtgtcactc gtggtgctga attggaagcg gcaatcaacg ctcttgaaga
    22741 tggacaagtt ctcttggttg aaaacactcg ttacgaagat gttgacggca agaaagaatc
    22801 taaaaacgat cctgaacttg gtaaatactg ggcatcactt ggagatggta tcttcgtaaa
    22861 cgatgcattc ggtacagctc accgtgcaca cgcatctaac gttggtatct cagcaaacgt
    22921 tgaaaaagca gttgctggtt tccttcttga aaacgaaatt gcctacatcc aagaagcagt
    22981 tgaaactcca gaacgtccat tcgtggctat ccttggtggt tcaaaagttt cagacaagat
    23041 cggtgttatc gaaaacttgc ttgaaaaagc tgataaagtc cttatcggtg gtgggatgac
    23101 ttacacattc tacaaagcac aaggtatcga aatcggtaac tcacttgtag aagaagacaa
    23161 attggatgtt gcgaaagctc ttcttgaaaa agcaaatggt aaattgatct tgccagttga
    23221 ctcaaaagaa gctaacgcat ttgctggtta cactgaagtg cgtgacactg aaggtgaagc
    23281 agtttctgaa ggcttccttg gtcttgacat cggtccaaaa tctatcgcca aatttgacga
    23341 agctttgact ggtgccaaaa cagttgtatg gaacggacct atgggtgtat ttgaaaaccc
    23401 agatttccaa gctggtacaa tcggtgtgat ggacgctatc gtgaaacaac caggagttaa
    23461 atcaatcatc ggtggtggtg actcagctgc cgcagcgatt aaccttggcc gtgcagacaa
    23521 gttctcatgg attagtacgg gtggtggagc atcaatggaa cttcttgaag gtaaggttct
    23581 tccaggactt gcagccttga cagaaaaata agattttata aacaaatcaa agaagagagg
    23641 gatgaaagtt cctcttttct tttgcttaaa ataaaaacgc ttcctctcaa ctattactca
    23701 taaaaatcac cgatttatga taaaatggaa atagaaagtt gagattatga gttattttaa
    23761 aaaatataaa ttcgataaat cccagttcaa acttggtatg cgaaccttta aaacaggtat
    23821 tgctgttttt ctagttctct tgatttttgg cttttttggc tggaaaggtc ttcaaattgg
    23881 tgctttgaca gccgttttta gcctgaggga gagttttgat gagagtgttc attttgggac
    23941 ttcgcgtatt ctaggaaata gtatcggtgg actctatgcc ttggtcttct tcttattaaa
    24001 tacttttttc cacgaagcct tttgggtgac cttggtagtt gttccaatct gcaccatgtt
    24061 aaccattatg acaaatgtag ccatgaataa caaagcaggg gttattggtg gtgtagcagc
    24121 tatgttaatc attaccctat caattccaag tggtgagaca attttgtacg tgtttgtgcg
    24181 tgtattagaa acgtttatgg gagtttttgt cgcaattatc gtaaattacg atattgatcg
    24241 tattcgtctc tttttagaga aaaaagaaaa ataatgttac attttataac attatcaatt
    24301 gacgtttgtc tttttttaga ctataataga cagaaagaag gaaattgtaa atgaaggaaa
    24361 aagaatttcg ccgaaatatg gctgtttttc ctatcggcag tgttatgaag ttgaccgatt
    24421 tatcggcgcg tcagattcgt tattatgaag atcaagagtt gatcaagccc gatcgaaacg
    24481 aaggaaatcg tcgcatgtat tccttgaatg acatggatcg tctgcttgaa atcaaagatt
    24541 atatctctga aggttataat atcgctgcca ttaagaaaaa atatgctgaa cgtgaagcga
    24601 aatccaagaa agcggttagt cagactgaag tacgtcgtgc acttcacaat gaactcctcc
    24661 aacaggggcg ttttgcttca gtacagtcac cttttggtcg cggttaggca accgcaagta
    24721 gtcatagatt aaggagaaaa cttatgccaa tcacagctgc agatattcgt cgtgaagtca
    24781 aggaaaaaaa tgttaccttt attcgtctta tgttctcaga tattttggga accatgaaaa
    24841 acgtcgaaat tcctgctaca gatgaacagt tagataaggt cttgtcgaac aaggttatgt
    24901 ttgatggatc ttctattgaa ggttttgtac gtatcaatga gtcggatatg tacttgtacc
    24961 cggacttgga tacatggaca gtcttccctt ggggagatga aaatggaagt gttgcaggtc
    25021 tgatctgtga tgtctataca acagaaggtg aaccatttgc gggtgaccct cgtggtaatt
    25081 tgaaacgagc tcttcgtcac atggaagaag ttggattcaa atccttcaac cttggtccag
    25141 agccagaatt cttcctattt aagttggatg aaaatgggga cccaacactt gaagtgaatg
    25201 acaagggtgg ctactttgat ttggcaccta ctgaccttgc ggacaacaca cgtcgtgaga
    25261 ttgtgaatgt cttgaccaaa atgggatttg aagtagaagc gagtcaccac gaggttgcgg
    25321 ttggacagca tgagattgac tttaagtacg atgaagttct ccgtgcttgt gataagattc
    25381 aaatctttaa gcttgttgtt aaaaccattg ctcgcaaaca cggactttac gcaacattta
    25441 tggcgaagcc aaaatttggt attgctggat caggtatgca ctgtaatatg tccttgtttg
    25501 atgcagaagg aaataacgcc ttctttgatc caaatgatcc aaaaggaatg cagttgtcag
    25561 aaacagctta ccatttccta ggcggtttga tcaagcatgc ttacaactat actgccatca
    25621 tgaacccaac agttaactca tacaaacgtt tggttccagg ttatgaagcg cctgtttaca
    25681 ttgcttgggc tggtcgtaac cgttcgccac ttgtgcgcgt acctgcttca cgtggtatgg
    25741 gaactcgtct tgagttgcgt tcagtggatc caatggcgaa cccttacatt gctatggctg
    25801 ttcttttgga agttggtttg tatggtattg aaaataaaat cgaagcacca gctcctatcg
    25861 aagaaaatat ctacatcatg acagcagaag agcgcaagga agctggtatt acagaccttc
    25921 catcaactct tcacaacgct ttgaaagctt tgacagaaga tgaagtggtt aaagctgctc
    25981 tcggagatca catctacact agcttccttg aagccaaacg aatcgaatgg gcaagttatg
    26041 caaccttcgt ttcacaatgg gaaattgata attatttaga cctttactaa tactaatata
    26101 gaagaaagat tgcctgtggg taaacagtca cacagcagtc taagccatca aatacctata
    26161 acccttaaaa tagcgatttc ggtcgctatt tatttttcga aaaatcctct tgactctttt
    26221 gcgaattaga tttatcatca gacgtgtaga agaaaatcaa ggagtcacta tggaaaaatt
    26281 caatttcaaa aacaatatcg ggcaagaaaa caagttactt caaatcgaaa tctacaagtt
    26341 tactaacttt tgcaagcttc aaaattacac atctgtaaat attttttcga aagatatatt
    26401 tgaagccata gtaaattgag actagaatag tacactacat ctgctaaaaa tttctagaaa
    26461 ttaatttaac tttcctaatc gatttgttta catcttattt caatctacta tattatctca
    26521 ataaatgatg atggcgactg ttttgtttgt gcttgtcgat ttttgaatcc tgatcgagcg
    26581 gaagaatatt tctgccgagg ttgtcaaggg gaaaatcctg aagatattga attttatgat
    26641 gaacaattac aggcagaaaa ggtggaggct ttgaatattc gattggaagt aaaaaattga
    26701 attatctaca atctcatcaa caataaacct atttcaaccc cattatctat aggcgtattt
    26761 ttacgccttt ttatatactt gaagcacata agaaacatta tgggaagttt gctgatggaa
    26821 gtactcaaga aattgatgtt ccttatgata ttcctgatac ttgggagtgg gtgaggataa
    26881 aatcaattta ttggaatttt gggcaaaata agccagagaa atcctttagg tatatagata
    26941 cgtctagtat tgatagaaaa aagaacataa tcaactacaa aaatctacaa tatctttcac
    27001 ctgaacaagc gccttcccgt gccagaaaat tagtttcgca gaatagtgtc ttattttcaa
    27061 cagttagacc atatctaaaa aatattgctg tagttagaga acttaaagag tatttgatag
    27121 ctagtacagc atttattgtt ttggatactt tacttaacga aacatatttg aaatattatt
    27181 tattatcaga taattttatc aatcgtgtta ataataaatc tactggaaca agttatcctg
    27241 caatcaatga ttataatttt aatctattat taatt
//
LOCUS       DAVBBS010000026        26070 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVBBS010000026 DAVBBS010000000
VERSION     DAVBBS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26070
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.026"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..177
                     /locus_tag="VQU32_001072"
     CDS             <1..177
                     /locus_tag="VQU32_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2472535.1"
                     /translation="VEDKAYNHALSKERSKVENIFAKVKTFKIFSTTYRNHRKRFGLR
                     MNLSAGIINHELGF"
     gene            complement(340..591)
                     /locus_tag="VQU32_001073"
     CDS             complement(340..591)
                     /locus_tag="VQU32_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472536.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNVEWSHADSYFLS
                     FVSDDVEERYIENVYLDSLSVKQKFKFIFDFGDEWRFEC"
     gene            complement(881..2761)
                     /locus_tag="VQU32_001074"
     CDS             complement(881..2761)
                     /locus_tag="VQU32_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET2472537.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(2755..3624)
                     /locus_tag="VQU32_001075"
     CDS             complement(2755..3624)
                     /locus_tag="VQU32_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2472538.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPDLENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNILCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQDVKKAVDNFVDTYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(3708..6353)
                     /locus_tag="VQU32_001076"
     CDS             complement(3708..6353)
                     /locus_tag="VQU32_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HET2472539.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDERFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELIVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAFRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVFRYYNMSQENVHISEHQQTILDLLE
                     RPYPVHSGPLDPQEIRTEFIKKEEI"
     gene            complement(6444..7724)
                     /locus_tag="VQU32_001077"
     CDS             complement(6444..7724)
                     /locus_tag="VQU32_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HET2472540.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSIDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            7895..9979
                     /locus_tag="VQU32_001078"
     CDS             7895..9979
                     /locus_tag="VQU32_001078"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HET2472541.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKTSAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGKAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKENKWLDIHTEQNHSHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPNH"
     gene            complement(10019..11698)
                     /locus_tag="VQU32_001079"
     CDS             complement(10019..11698)
                     /locus_tag="VQU32_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HET2472542.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNLDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFDCRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIYVLAFQAGETEKRLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            12453..13682
                     /gene="arcA"
                     /locus_tag="VQU32_001080"
     CDS             12453..13682
                     /gene="arcA"
                     /locus_tag="VQU32_001080"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HET2472543.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELILSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGNLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            13740..14756
                     /gene="argF"
                     /locus_tag="VQU32_001081"
     CDS             13740..14756
                     /gene="argF"
                     /locus_tag="VQU32_001081"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET2472544.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            14921..15868
                     /gene="arcC"
                     /locus_tag="VQU32_001082"
     CDS             14921..15868
                     /gene="arcC"
                     /locus_tag="VQU32_001082"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HET2472545.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVIVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            16077..17588
                     /locus_tag="VQU32_001083"
     CDS             16077..17588
                     /locus_tag="VQU32_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HET2472546.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYEA
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNTWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            17610..18941
                     /locus_tag="VQU32_001084"
     CDS             17610..18941
                     /locus_tag="VQU32_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET2472547.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNGLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAEDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            complement(19347..19637)
                     /locus_tag="VQU32_001085"
     CDS             complement(19347..19637)
                     /locus_tag="VQU32_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472548.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
     gene            complement(19625..19876)
                     /locus_tag="VQU32_001086"
     CDS             complement(19625..19876)
                     /locus_tag="VQU32_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HET2472549.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGDQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            complement(20275..21159)
                     /locus_tag="VQU32_001087"
     CDS             complement(20275..21159)
                     /locus_tag="VQU32_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET2472550.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(21304..22455)
                     /locus_tag="VQU32_001088"
     CDS             complement(21304..22455)
                     /locus_tag="VQU32_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET2472551.1"
                     /translation="MAIFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHRCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPQALTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIEHENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIVEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(22601..24367)
                     /locus_tag="VQU32_001089"
     CDS             complement(22601..24367)
                     /locus_tag="VQU32_001089"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HET2472552.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGCAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDSGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            complement(24424..>26070)
                     /locus_tag="VQU32_001090"
     CDS             complement(24424..>26070)
                     /locus_tag="VQU32_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET2472553.1"
                     /translation="NYRTDKTAMWALSGNFDASKSWKKEELELANWISKNYSINPVDN
                     DFRTTTLTLKGHTGHKPQINISGDKNHYTYTENWDENTHVYTITVNHNGMVEMSINTE
                     GTGPVPFPTPDKFNDGNLNIAYAKPTTQSSVDYNGDPNRAVDGNRNGNFNSGSVTHTR
                     ADNPSWWEVDLKKMDKVGLVKIYNRTDAETQRLSNFDVILYDNNRNEVAKKHVNNLSG
                     ESVSLDFKEKGARYIKVKLLTSGVPLSLAEVEVFRESDGKQSEEDIDKITEDKVVSTN
                     KVATQSSTNYEGVAALAVDGNKDGDYGHHSVTHTKADSNAWWQVDLGEEFTVSKVDIY
                     NRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDKVVDGLLSLKVPSVGAKLVKIELKSAA
                     IPLSLAEVEAYGSKRTPKKLSNIALTKETRQSSTDYNGFSRLAVDGNKNGDYGHHSVT
                     HTKGDSPSWWEIDLAQTEELEKLIIYNRTDAEIQRLSNFDIIIYDSNNHEVFKQHIDS
                     LESNNLSIDLKGLKGKKVRISLRNAGIPLSLAEVEVYTYK"
BASE COUNT         7400 a         5822 c         5085 g         7763 t
ORIGIN      
        1 gttgaagata aagcctataa ccatgcgcta tccaaggaga gaagcaaggt tgagaacatc
       61 tttgccaaag taaaaacgtt taaaatattt tcaacaacct atcgaaatca tcgtaaacgc
      121 ttcggattac gaatgaattt gagtgctggt attatcaatc atgaactagg attctagttt
      181 tgcaggaagt ctattgaaat actaagccta cacagactga tttcaatttt accattcttc
      241 atagtcaaaa ccatcataat ctggatattg ttctggcaac gttccaaccg aacgtacgag
      301 ataagcttct tcgtcctctg tctcgatttc tctcagcact tagcattcaa aacgccattc
      361 atcaccgaag tcgaaaataa acttaaattt ttgtttgaca cttaggctat ccagatagac
      421 attttctatg taacgttctt caacatcgtc actaacaaag ctaagaaagt aagaatctgc
      481 atgactccac tcaacattat ccatgaaaaa tgcatgagca tgatcatttg caaaatcgaa
      541 actccataaa atcacatctg ctagttccgc taacgttttt gtttttggaa tctatatatg
      601 tcggtagcaa ccttttttaa tttgacggaa atgatccaag cttctacaga tgctggcttt
      661 tcagcttcta tttcttgttt tactaatttt aactgtagca tttgaaacca gataattctt
      721 ctgtttcacg cgaaacaccc tgcgccaagc aaggtgttct tgtttttata ctcaatgaaa
      781 atcaaagagc aaactaggaa gctagctgca ggctgctcaa aacactgttt tgaggttgta
      841 gatggaagct gacgtggttt gaagagattt tcgaagagta ttaagtcgta taaatcgtcg
      901 aagctgtcgc aatggtatgc cactggttag ctgtagttgc tgcgaaatcc ttgtctgcgt
      961 agaagtcagt aaatggtagg atttcaactt ccagctcgtc gatgcggtca agctggccag
     1021 ccagataaac ctcgatacgg ctttctgctc gtttgatgcg ttgcaagagt ccgcccatac
     1081 ggatgtcaac tgtatccaaa ccaaagacct tgttttcttt cagccattgg tgactaaaga
     1141 gggcatggaa gtcttcaatt tggcttctaa gttctggtaa ttcttgtctg gcgatttgtt
     1201 gtaaactttc tttatcatcc gcttggtagg cctgacgaat gcgtcgtccc acatctactt
     1261 tgctacttaa aatagcattc aactgggcct gagtttcaaa gagataagca tagtttccag
     1321 ctttttcttt aatgttagca agcgtctcag cagcctgagc gaagtgcggt ttgtcctgtt
     1381 caggtgtcat gtgttgatca agaatcggac aaagaatatc ctgataaaaa acatagcggt
     1441 tgggattgat accgctgaga ttgcctggta ggtctggtaa gaggttggca aggtcaatct
     1501 gcataaaatc ctcaaccgtt agaccagtat tggtcttgaa gtgcgcagac aaaccatcta
     1561 ggtcattgcg atagctgagt tctgcccaga tttgcaagct tggtaggata gagaactggg
     1621 cagtttcacc accattgtct ccccaacccg ttacgatgac ttctttaatc tgattggcac
     1681 ggcaggcttt attagcctcg atagccacta gacggctaaa atggttgtga ggtgtaaagc
     1741 caatccactt ccaagctccc cctgcaaatg caaggtcatg gctaatcttg tgatgattgc
     1801 ggaaattacg gttgtatttt tcctcgctat cctgataata atcccagtaa accagagtca
     1861 cacggtcttt gagacggtct aggtagacac gagtttcctc tggaatttcc acatcacggt
     1921 cgtactggcc atccgctgac atgagtttga agaacatatc actccacatc tggcagtgga
     1981 aaccatattt gtcagcaata tccagcacgc gctccaagtg ttggcacatg aggagactac
     2041 gatccacaac accgttcaaa atcaggtagc gtcccaaacc aaccaagtgg gcttcgtcca
     2101 tcccgatatt gaccttgcga gtcttcagtt tagacaacgt ggcaaacatg ccatcaatca
     2161 ggtcataaac cttttcttcg ccaatgagaa gaatgtcctc tacatcacgg agctcctgca
     2221 cttccttgac accccatttg acaaaggccg acaagtgggc caaggtctgg atgcatggta
     2281 caaaggtcat gtcaaactgt tgagcatagg cttcgatttc ctgcaactcc tctgctgaat
     2341 aagctccacg gaaatagcca aagtaaggct gcccttcaat ctggtaagtg tcttccatgt
     2401 aaagttcaaa ggttgagtag cccatgagag ccaagacctc aatcatctgc ttggcagaag
     2461 ccacattcag caccgcattt cgagaacagt caaccatgta agccaaatct tcgtaagctg
     2521 cttgttcctc aatctctact ttatcagctt ctgctagaac tgttaccaac aaggataagg
     2581 cacgataaag ttggtgaggt ttgcggtagg tcaattgata gtgaccttcc tcaccctgga
     2641 tagagataga ggcttggtca gactgagtga cagccacttc cacatctggt agagaaatat
     2701 gaccttttaa aacctcaata gcttgcgttt gtttgggact aagtcctgta aatcttacca
     2761 ttgcttttcc tcctgtagcc agttgacaag agcaccgtag agattggcat ctgcgtgata
     2821 ggtgcaggcc tggataactg gtgcgaccgt gtattcttcg taggtatcga caaagttatc
     2881 aacagccttc ttgacatctt ggataaaatc tggattttga ctgatagagc cacccagact
     2941 gatgacatct ggatcgatca gatactggat attgagcaag ccttgcgcca gattgcggtt
     3001 catgcgctca atggcttctt gacaaaggat attaccagct gcggcctctt ggtaaatctt
     3061 gcgaccgtcc caatcagtat gaccagattt ttcaatcacg tatcgtacca tattcccagt
     3121 tgatgctagt tgcgaccagt tattaagttt ttcagcaggg gcaagggttg tcatgtagcc
     3181 aaattctcca cccaagccgt ggcgacctcg atgaagtcta ccattgataa tcatggctcc
     3241 gccaatccct gtcccaatca cgacacaggc tgcattttca agatctggat gagctagtag
     3301 ttcactgagt ccaacgcagt tggcatcatt ttctaaatgg acaggtagct gataagagct
     3361 aagcgcctca taccaagaaa agccatggat gtagggcacc gcactgaagc catcaatcac
     3421 acctgtctct tgattgactg cacctggaac gctcatagca atcccactgt aatcctgttc
     3481 tgacaagcgt tgatctagcc acgctagtaa atcctccaag ttttcaggcg ttgaaatact
     3541 tgtcttatcc agtattttcc catcaggagt cagactggca aacttaatcc cagtccctcc
     3601 gatatcaatc gttgcaatgg tcattgtttt taccataaaa aggggcgaat cagcagttag
     3661 ttcttacttt ttcgcccctc ctttcttttg atgtttactt tttgaaatta aatttcttct
     3721 tttttgatga attctgtacg aatctcttgt ggatccaatg gtcctgaatg aactggatat
     3781 ggtcgctcaa gtaagtcaag aatggtttgt tgatgttctg atatgtgcac attttcttga
     3841 ctcatattat agtaacggaa gacatagcct tcttcatttt ccgccacttt aaaggctgtt
     3901 ggacaaactt gcggtatgct gagaacagaa tggctcaaga ggctaccagt cgcaaccacg
     3961 cttccttcct gtctagcaag ctgaaggctg gtaaacggtg tctgcaaggc tttggcacga
     4021 cggaaagctg aaaagcgttc ttgggcttgg tggcattcaa gtgcaaactc gacttcaaac
     4081 tcacgcaagc attgtgcttc tggcgttggg aagtagcccc agtcacctag ctcacctgat
     4141 gcacgcaaaa tggtcacggc aatggtgtta tccccaagga tttcgtattc attcaatccc
     4201 ttattggata cagtcacccc tttttcatcg tcatacagac tgacaaaagc ttgttggtgt
     4261 tgaggatttt caggattttc ccatgaagca gctggtttgt ttggtcgtgt caccacctca
     4321 tagatacttt cagaatcatt gcttggacgc gtgttatgag tcttgaccaa gagacggata
     4381 cggtgatcct tggcagtgtt agtaaagcga gtcttgaagc ggatttgtgg attgtcaaca
     4441 aagacaatca actcagtttc cagaggaatg tttgtcaatt cttctgaccg tccagcctca
     4501 cgcttcataa actcgatgat accttgttgc tcttcttcta gcttttcatc cgcactgaca
     4561 ggcacggtca attcatgttt gagcaagatt ttagcatagc gatctgtgtt ttccaagacc
     4621 tcgtggccct taagctctgc aaagattggc tctgttcctt ttggttggaa atagatatac
     4681 tcgtttccga tgtccccacg gtcttcgaag cggataaagt cttcataggc ttcgtgagtt
     4741 gtcttgtcat atactgtgat attgtcatcc acactcaccg ttacgaatgg tgtatcaatc
     4801 actccgtttt ggtaaatacc ctcacggtgt tcttgttttc cttccagcaa ttggaaggtt
     4861 gtccaagaaa gcggcgctag gtgaactgga atggtcacgc gcacttgtcg agcaatacga
     4921 gcttggcgga acttgtcttt tggtaaatcg tactcaaaat tagctccgag gtcttcgatt
     4981 gtagcctcta caggacgacc atccaagtcc tccacacggt aacttggtaa gataagagca
     5041 gccatctttt tgtagccttc tgttgggtgc aattccttga aatcacaagt cgccacatca
     5101 atcactgtgc tgacagtatc gaccttatca tgcaagcctg tgttaatgac agtaaagaga
     5161 tagtcacttt gagccttatc cgtagcaatt ttacccttcc actcgttgag caagttactt
     5221 ttaacaaagt ttcctacttg gttgaccttg gcaaaacgcg tttccatctc gcggtgaact
     5281 tcgtccacgc tacagccaca gatactatca tgtggcgcat tctgcaaaag tgttttccaa
     5341 gcataggtca actggtcctt gtggttgtgt ccaccagtga taatagtcaa gggttctacc
     5401 acttgctcta ggaggttgct attttcttgg aaggcttgtt ttaggtaaat gcgggatgaa
     5461 gaagtgttgg caagtgtgta ccagccatct gtttcctgac tggtcaactc gcctgtaaca
     5521 gttgataagt gttcaggaag cgcaccttct acagcttgaa catattcatc aaaagaacta
     5581 tgaacaaaga ttacatccgg gaagagttca tttgccacac gaatggcttc gctcagattt
     5641 ttctgtacag gctggtggtc acagccgttc atcatcaacc attggttggt cgaagcgtag
     5701 gcacgcacat ctgacaattt ttgtttccag aaggtcaagg cctcatcttt gtcaactggg
     5761 atttcattcc cattactgta ccagttggca aagagaatac ctaaaacacg actaccatcc
     5821 acaccctgcc agtacatttc tgaaaactgg gatgtaaacc gctcatcttc aaggacttgg
     5881 ttgtcaaatc caatcggctt cacaccacga ccaaaggctg ccacgtgaat acctgatttt
     5941 tgaaggattt gaggagcttg tcccatattt ccaaaggtat ctggaaagta gccaatctgg
     6001 gttgatttac cccatttggc agcttcttgt tgaccaatca aggtattgcg gacattggct
     6061 tcactggaga tcaagtagtc atcctgcaag atgtaaaagg gaccaatttt aagtttgccc
     6121 tcgtcaatgt agcgttggac cttgtcgcga ttttcagggc gaatttgtaa gtagtcatca
     6181 aggacaatag tttgtccatc caagtggaaa ctcttgaact cagggtcatt ttcaaagaga
     6241 tcaaagagat tgtcaaacaa ttccaccaac tgcatacgat ggctttcaaa aggcaagtac
     6301 cactcacgat cccagtgact atgtgagata atatgtacaa caacattttc catgaagtaa
     6361 aacctcattc taaatttaaa ttttaacgtt ttaaatgtaa acttgtgatt ctgataagaa
     6421 tcggttgagc taaagcgagc cccttagcgg atatccaagt aatccaagac caactcacag
     6481 aacatcatgt tagcccagga gaaccattca cgagagtaga gggtcggatc atctacgtgg
     6541 aagctttcgt gcatgacacc tgtaccacca tcgcaggcaa ccagctgatc cagcaagaat
     6601 tttttctctg ccttatctct tgttgtcaat ccttggatag aaagggcgat tggccagata
     6661 tagcgataga aggtatgaga acttccgaga ccgctagcgt attctccttg gtagaagtat
     6721 ggattttcag agctcagaat ggtacggcga gtggcttgat acacttcatc atcgatcgaa
     6781 cagtagccca gatagggcgc agctagtaga cttggtacat ttggatcatc catgatgctg
     6841 gcatttccta ggccatctac ttcaaaagcg taaatctttt cgcccttgct gttggtggtg
     6901 taagcgtagt tttcgattcc ttcttggatt tcatcctgaa gacgcttggc atcagcaata
     6961 acactctggc tatcagctag gtttaatgct gcgaagattt cttgcacata acccaagact
     7021 actacagcaa acatatttga cggaatcaag taactatact ggcaacagtc atcactcgga
     7081 cgaaaagctg accaagtcat acctgtcact gcaaagtcag gtccaaagcc atcatttacc
     7141 aaggtgtctt ccttacggtc cgtatctcgg acaaaacgat aaggagagtt cttgtggtct
     7201 tgttcaactg tccatagatg gagaatttcc ttagtcgctg cgacaaaagt ttcatcaaac
     7261 tgactagtct cgccagtctc tttccagagg agataagcca actgcaaagg atagcaaagc
     7321 gaatccacct catacttgcg ctcccagatc cagccgttaa ggtctgtgtg gtcagtctcg
     7381 tggtgccctt tccagttctc ctcaatgttg aaggagttag catagggatc cttgagtacc
     7441 aaggtcatct gacgtttgac caaacctgca atggtctgac gcaggagggc atctctttta
     7501 gctacatgaa ggtagggtct gagttgggct gtcgaatctc gaagccacat ggcaggaata
     7561 tccccagtca agacaaaagt tgaaccatct tctaagattt caaccgtatt gtccaaggta
     7621 tcggtgtagc aacgctcaaa gacatcaacc cactctggat ggtccttggc ccgctctgct
     7681 acttcatcta gccattctct aacaatttct ttcgaataaa tcatcgtgca attccctctt
     7741 tcaaacaagt ttcatgttca gtatatagct tttgagacag aaaatacata cacaaatgat
     7801 agtcattttt ctacaaaata gttatcttta aaactccttt tctaaaggcc ctctttttct
     7861 gatataatgt agaaaaacag ctaaaggaaa gactatgaaa ccactacttg aaaccatcga
     7921 tacccgcttt ggtactgcta gcaagcatgc cttctctcgg ggaaataccc tgccatacac
     7981 aggcgtgcct tttgggatga attactttgt gccccagacc agtgaccagg atggatcttg
     8041 gttttttgat ccgcatctgc ctatctttca ggggattcga ttaactcacc agcccagtcc
     8101 ttggattggg gactactctt ggctccttct gacacctgtc accagtcaac tggggggaga
     8161 cagcctcttt caccgccagt cttcttatga tatagataaa gcttgcttcc agcctcacta
     8221 tttgaagctc ttttctctgc gttatcagat tgaaacccag ctcacaccga cttgctatgg
     8281 tgcttctatt cgtttgaatc aaaagcaagg caaagccctc tccctctatc ttcacgcagc
     8341 agatgaactg acagtagagc aagtagataa gcggactctt gccctgcgac aagaaggtaa
     8401 aactgaaact aacaaaaatt cgctaacgat gttcactgcc ctgcaaatga acacggatat
     8461 tcttgctatc agccaagaag ctggggactg gcgaattgac ttagcaagta gtcaaaccga
     8521 gatgcaacta gccacttctt tcatctctcc ttctcaagct ctaatcaatc tacctcaaga
     8581 agattttgat agctgtaaaa caagtgccca agcggattgg gaaaatctcc tccatcgttt
     8641 tgacattata gagacaggag aggctgaccg aaccttcttt gaccactgcc tctacagact
     8701 cttcctcttc ccacagactt tttatgaggt tgatgagtca gggaaagcca tccacatgga
     8761 tctggctact ggtactgtca agcctggtgt cctctttagc aacaatggtt tctgggatac
     8821 cttccgcacc accttccccc tctttgccct tatcataccg gagcactatc aacgcttttt
     8881 agaaggtttc ctcaatagct accgcgatac tggtttcctt ccaaaatggc tggctccaga
     8941 tgaacggggt atgatgccag gtacactgct agatggcatt atcgcagata gcgcctgtaa
     9001 ggacatggcc cccgacctag aaggagaact cttccaagcc atgctcgaaa cagccagcaa
     9061 agccgaccct ctcggcatca atggccgcca cggactagcc caataccaag aactaggtta
     9121 cctctctacc gaccaccacg aaagtgttag ccatactcta gactatgcct atagtgactt
     9181 ttgtatcgcc agctgtgcca aaaaactaga gaacatagaa atcgctgaaa cctacaaggc
     9241 tgcttcacaa aattaccgcc agctatttga cgctgagaca ggttacatgc gagcacgaga
     9301 caatcaagga aactttcacc ctgacttctc tccttatagt tgggggcgag actacgctga
     9361 atgctctgcc attcaagcta ctttaggcgt tctccacgac atccctggct taatccaact
     9421 gatgggtgga aaagaaacct ttagcaacta tcttttgaaa gcctgtcaag atgctcccct
     9481 ctttgaaaca acaggctatg gttacgaaat tcacgaaatg agcgagatgg ctactgctcc
     9541 ttttggacaa atagccattt ccaaccagcc tagtttccac attccttatc tcttccgcta
     9601 cagcgattac cctgactaca ctgcccttct tatcaagacg ctccgtcaga aagcttttca
     9661 cccaagttgg gaagcctatc ctggagatga agacaatggt agtctctctg cttggtacat
     9721 ctggtcagct ctcggatttt atccaacctg tccaggaaaa ccaagctatg atctcggaat
     9781 ccctctcttt gaccatctac gagtctacct agctaaagaa aataaatggc tagatattca
     9841 tactgagcaa aaccacagcc attttaactt tgtcaaagaa tgccgactgg acaaaacact
     9901 cgtatcaact attcaacacc aagacctcct aaaagctgaa caactaactt tcaccctcag
     9961 ctggctacca aatcactaat aaccaaaaga acctttgcat cgcgcaaagg ttcttctttt
    10021 atgaaacttg gacttgaagt tgatccacaa gctgaccatc caccgttagg ttcagataga
    10081 aatccaaggt tttatctcct gcgaaataca aggttggtag cgtcagcctt ttttcagtct
    10141 caccagcttg aaacgcaagg acataaatct cgtcctgata ggcgacacca tggacacctg
    10201 tccccggttg aatcgaaatc ttagcctcta aagaggagct actttcacta cgttcaatcc
    10261 taaaatgaag agtctctccc tttgccacag ctaggctttt ttctgcaaat gctagttcct
    10321 gaacaacttc tttttttgat aacccaggag ttttataaag ggaaatcttg gtcaatacag
    10381 ggatagattg tgcctcggta atcatcacgc gcaccttttg tgcctctact agcggacctc
    10441 gcaagaggcg cttatgacca actgtgaagc ccctgccaaa ctcctgccag acaccatcca
    10501 cctctacttg cacatgaaaa gcagcgattc gttgccctag cttcaaatct tctcttaact
    10561 caattacatc aaaagtttta ggagaaccta aatcgagttc taactggatg ggcaagtctg
    10621 catcgcttgc ccaagagctg gtctcaaggc cgtctgtcaa atggcgacaa tcaaagtctg
    10681 cggaaagagc tggaccagat acctcagctc ccagagccaa atcttcttta tagagctcat
    10741 tgcgataggt cgcaaattca taaagtcgtt caatatcctt tgcatcaaag agcccagctt
    10801 gattcggcgg aatattaagc aagagtggag ttcctcgccc tactgagtga aagtagattt
    10861 cgaccaactc ctcgagagac ttaggatcct gatcctcatg gtagaaccag cctggacgga
    10921 tggaaacatc tgcctctccg attgaaaaaa tcgtgcccga gggatccccg tgctgaagat
    10981 agttcagctc tgcttctgtt cctagtttat caagattcac cttttgccac agtggatcac
    11041 ctgcataccc tcgttcattg ccaatccagc ggatactggt gccttctgtt gaaaaaatca
    11101 agcaatcgcc ctgcaggtca cgaatggttt caaaccattt ttcaaattca taattaacct
    11161 tttgcgcgcc ctctcctctg gcaccatcca tccaaacctc agcgaactta ccagcattcc
    11221 catagttagg atttgataag atttccttca actgagccag ataataggca ttgtagtccg
    11281 cttctcggtc cacatgatag aggggactat gggcatccca cggtgacagg tagaccccca
    11341 tatccatatc aaactctgtg gcagcttggg atacttcaag gagcaagtcg ccctttcctc
    11401 tcctccaagg actgacctta accgaataat ctgtgtgagc tgtcggataa aggacaaagc
    11461 catcgtggtg cttgaccacc aaaatcaact ttttgaagcc cgtttccttg agcacacgaa
    11521 cccactcacg cgcatccaac tgactcgggt taaagcgctc aggatcctcc tgtccagtcc
    11581 cccattcttg gtcataaaag gtattaggac cgaagtggat aaaagctgct agttcatctc
    11641 ccagataagc tagctgagtc tgacttggta acggtccatg cggtttgatt ttcttcatcg
    11701 ttatatatct ccttcttttg caacttctct tgttcacaag gttatccaca ggttgtggat
    11761 aataaaccat gatctaacag ccagctcttt ccaactccaa ctggtaggct caggattttt
    11821 ctcctcaatg ttccctcttc aaacagctct gtcatatggt tctgctaaca cctctcagaa
    11881 tgctactctc cataattatt ctcaggcttc tttcaggtca ttgtgtgaag accattcact
    11941 ctccagactt ttccacagat tgtgaataag tgattattct aagctatcac ctcgtcgact
    12001 ataacaaaaa ataaaaagag ggtcaataag ataattatct cttcctaaat actcagaaaa
    12061 gattctataa tttgtacaat tgaacaagaa atgcttctcc aaaatctatc ttcaaaagaa
    12121 tgttgcatct ttttatcctt tcacacaaac atttctcttt atacagatcc aagtgataac
    12181 cagatgataa ttggacactt tttggtcatt tgtatatttt ttgaaaaatt ttttaaaaaa
    12241 attttatatc ctttttatag aagtttatgc atttgtatct atgtattcta tcctatgtta
    12301 tgtataataa cataaaattg tgctaaacat cataatttat attattttcc gagaaattat
    12361 agccattgac attacctagg aattgcgtta taacatacat gtaagcggta cccaaaatgg
    12421 tgccaagtca aaatttttaa ggaggaaaat acatgtcttc tcatccaatt caggtcttct
    12481 cagaaattgg gaaactgaaa aaagttatgt tgcaccgtcc aggcaaggag ttagaaaact
    12541 tgttgccgga ctatcttgaa aggcttcttt ttgatgatat tcctttcttg gaagatgctc
    12601 aaaaagaaca tgatgcattt gcccaagctc ttcgcgatga aggaattgag gttctctacc
    12661 tagaacaact cgctgctgaa tcattgacct ctccagaaat ccgcgatcaa tttatcgagg
    12721 aatacttaga cgaagccaac atccgtgatc gtcaaaccaa ggttgctatt cgtgaattac
    12781 ttcacggcat caaggacaac caagaattgg ttgaaaaaac aatggctggg attcaaaaag
    12841 ttgaattgcc agaaattcct gacgaagcta aagatctaac tgacttagtt gaatcagatt
    12901 atccatttgc aattgacccg atgccaaacc tctatttcac tcgcgaccca tttgcaacaa
    12961 ttggaaacgc cgtatcgctt aaccacatgt ttgcagatac tcgtaaccgt gaaacactct
    13021 acggtaagta tatcttcaaa taccacccaa tctatggcgg aaaagtggat ttggtctaca
    13081 accgtgaaga agatacgcgt atcgaaggtg gagacgagct aattctttct aaagacgtcc
    13141 ttgcagtagg tatctctcaa cgtacagacg cagcttctat cgaaaaactt ttggtcaaca
    13201 tcttcaagaa aaatgttggc ttcaagaaag ttttggcctt tgaatttgct aacaaccgta
    13261 aattcatgca cttggatact gtcttcacta tggtagacta tgacaagttc actattcacc
    13321 cagaaatcga aggcaacctt cacgtttact cagttactta cgaaaacgaa aaacttaaaa
    13381 ttgttgaaga gaaaggtgac ttagctgaac ttcttgctca aaaccttggt gtagaaaaag
    13441 ttcatttgat tcgttgcggt ggtggcaata tcgtagcagc tgcgcgtgaa caatggaacg
    13501 acggttctaa cactttgacc atcgcacctg gtgtggtagt tgtttatgac cgcaataccg
    13561 tgaccaataa gattttggaa gaatacgggc ttcgcttgat taagattcgc ggaagtgaat
    13621 tggttcgggg ccgtggtgga cctcgttgta tgtctatgcc atttgaacgt gaagaagtgt
    13681 aatcgctgtt cggtattcgt caatagaaaa tgtaaaaaat agaaagagga aataataaaa
    13741 tgacaaattc agtattccaa ggacgcagct tcttagcaga aaaagacttt acccgtgcag
    13801 agttagaata ccttattggt ctttcagctc acttgaaaga tttgaaaaaa cgcaatattc
    13861 aacaccacta ccttgctggc aagaatatcg ctctcctatt tgaaaaaaca tctactcgta
    13921 ctcgtgcagc ctttacaact gcggctatcg accttggtgc tcacccagaa tacctcggag
    13981 caaatgatat tcagttgggt aaaaaagaat ctactgaaga tactgctaaa gtattgggac
    14041 gtatgtttga cgggattgaa ttccgcggat tcagccaacg tatggttgaa gaattggcag
    14101 aattctcagg cgttccagta tggaacggtc taactgacga atggcaccca actcaaatgc
    14161 tcgctgacta cttgactgtt caagaaaact tcggtcgctt ggaaggcttg acattggtat
    14221 actgtggtga tggacgtaac aacgttgcca acagcttgct cgtaacaggt gctatccttg
    14281 gtgtcaatgt tcacatcttc tcaccaaaag aactcttccc agaaaaagaa atcgttgaat
    14341 tggcagaagg atttgctaaa gaaagtggcg cacatgttct catcactgaa gatgctgatg
    14401 aagcagttaa agatgcagac gttctttaca cagacgtttg ggtatcaatg ggtgaagaag
    14461 acaaattcgc agaacgtgta gctcttctta aaccttacca agtcaatatg gacttagtta
    14521 aaaaagcagg caatgaaaac ttgatcttcc tacactgctt gccagcattc cacgatactc
    14581 acactgttta tggtaaagac gttgctgaaa aatttggtgt agaagaaatg gaagtaacag
    14641 acgaagtctt ccgcagcaag tacgctcgcc acttcgatca agcagaaaac cgtatgcaca
    14701 ctatcaaagc tgttatggct gctacacttg gtaaccttta tattcctaaa gtataatttt
    14761 agataataaa ccgtctacca acagctatga gggctgcgac taatagcttt agtccggtcc
    14821 tcttttatgt aatggtaatc tattatttct tataaaatat gtgaaaaatc attaaattga
    14881 aatctaaacg cattctattg agtgtgataa aggagaattt atggcaaatc gtaaaattgt
    14941 agtagctttg ggaggaaatg cgattctttc ttctgaccca tcagcaaagg ctcaacaaga
    15001 agctttagtt gaaacagcta agcatcttgt aaaattgatt aaaaatggag atgatttgat
    15061 tatcactcac ggtaatggac ctcaagttgg gaatctcttg ctccaacatt tggcatcaga
    15121 ctctgaaaag aaccctgcct tcccactcga ctcacttgtc gctatgacag aaggtagcat
    15181 cggtttctgg ttgaaaaatg ctttgcaaaa tgctctcttg gatgaaggca tcgaaaaaaa
    15241 tgttgcctct gttgtaacgc aagttatcgt agataaaaat gatccagctt ttgttaactt
    15301 gagtaaacca atcggtcctt tctattcaga agaagaagca aaagcagaag ccgaaaagag
    15361 cggagcgact ttcaaggaag atgctggccg tggctggcgt aaggtcgttg cctcaccaaa
    15421 acctgttgac atcaaagaaa ttgaaaccat ccgtactctt ttaaataatg gtcaagtcgt
    15481 cgtagctgca ggtggtggcg gtattcccgt cgtcaaagaa aacaatggac atttgactgg
    15541 tgtcgaagcg gttattgata aagacttcgc ttcccaacgt ttggcagaat tggttgatgc
    15601 agacctcttc atcgttttga caggtgtaga ttatgtattt gttaactaca acaagccaaa
    15661 ccaggaaaaa ttggaacatg tgaatgttgc ccagctggaa gaatatatca aacaagatca
    15721 gtttgcacca ggtagcatgc ttccaaaagt agaagcagct atcgcttttg tcaatggtcg
    15781 tccagaagga aaagcagtta ttacttccct tgaaaatcta ggcgccttga ttgaatctga
    15841 aagcggaaca attattgaaa aaggataagt tgttttacta ataagatgta ttctatttct
    15901 agtatcttta tatcaaatta gaaattattc ttgaaaacat gtacaatatt tcaaaagata
    15961 ctagttttag actttaatat ggtaaaacaa atataaatag aaagcgtttt cttgaatgtt
    16021 tatttaagaa agtagttggt tttttacact ttgttagaca tcaggaggaa aaacaaatga
    16081 gtgaaaaagc taaaaaaggg tttaagatgc cttcatctta caccgtatta ttgataatca
    16141 ttgctattat ggccgtgcta acttggttta tccctgcggg ggcctttata gaaggtattt
    16201 acgaggctca gcctcaaaat ccacaaggga tttgggatgt cctcatggca ccgattcggg
    16261 ctatgctagg tactcatcca gaggaaggtt cgctcattaa agaaacgagc gcagcgattg
    16321 atgtagcctt cttcatcctt atggttggtg gtttccttgg cattgtcaac aaaactggtg
    16381 ctcttgacgt agggattgcc tctatcgtga agaagtataa gggccgcgaa aaaatgttaa
    16441 ttttggtatt gatgcctttg tttgccctcg gtggtacaac ttatggtatg ggtgaagaaa
    16501 caatggcctt ctatccactc cttgtgccag ttatgatggc cgttggtttt gatagcctga
    16561 ctggtgttgc aattattttg ctcggttctc aaatcggctg tttggcatct actctgaatc
    16621 catttgcgac aggtattgct tcagcgactg cgggagttgg tacaggggac ggtatcgtac
    16681 ttcgtctgat cttctgggtt accttgactg ctcttagtac ttggtttgtt taccgttatg
    16741 cggataagat tcaaaaagat ccgactaagt cactggttta tagtactcgc aaagaagatt
    16801 tgaaacactt taacgtagaa gaatcttcat ctgtagaatc tacacttagc agcaaacaaa
    16861 aatcagttct cttcttattt gtgttgacat tcatcttgat ggtattgagc ttcattccat
    16921 ggacagacct tggcgttacc atttttgatg actttaatac ttggttgact ggtcttccag
    16981 ttattggtaa tattgtcggt tcatctactt ctgcactagg tacttggtac ttcccagaag
    17041 gcgcaatgct ctttgccttt atgggtatcc tgattggtgt tatttatggt cttaaagaag
    17101 ataagattat ctcttccttc atgaatggtg ctgctgactt gctcagtgtt gccttgatcg
    17161 tagcgattgc tcgtggtatt caagttatca tgaacgacgg tatgattacc gatacaatcc
    17221 tcaactgggg taaagaaggc ttgagcggtc tatcttcaca agtctttatc gttgtaactt
    17281 atatcttcta tctacctatg tcattcttga tcccatcttc atctggtctt gccagcgcaa
    17341 ctatgggtat catggctcca cttggagaat ttgtaaatgt ccgtcctagc ttgattatca
    17401 ctgcttacca atctgcttca ggtgtcttga acttgattgc accaacatct ggtattgtga
    17461 tgggagctct tgcacttgga cgtatcaaca ttggtacttg gtggaaattc atgggcaaac
    17521 tcgtagtcgc tattattgta gtgaccatcg cccttcttct ccttggaacc ttccttccat
    17581 tcctataaaa tagtgagtga ggtgattcca tgaaaataga tataacaaat caagttaaag
    17641 atgaatttct tatatcatta aaaaccttga tttcctatcc ttcagtactc aatgaaggag
    17701 aaaatggaac accttttgga caagcaatcc aagatgtcct agaaaaaact ttagagattt
    17761 gtcgagacat aggtttcact acctatcttg accctaaagg ttattacgga tatgcagaaa
    17821 tcggtcaggg agcagagctt ctggccattc tctgtcattt ggatgttgtt ccatcaggtg
    17881 atgaagcaga ttggcagaca ccgccatttg aagcaactat caaagacggc tgggtattcg
    17941 gacgtggtgt ccaagatgat aaaggccctt cgctcgcagc tctctatgca gtaaaaagct
    18001 tgctggacca aggtattcag ttcaaaaagc gcgtacgctt tatctttggt accgatgagg
    18061 aaaccctctg gcgctgcatg gcacgctaca ataccatcga agaacaggcc agtatgggct
    18121 ttgcacctga ctcatctttt cctctgacct atgctgaaaa agggcttcta caggtcaaac
    18181 ttcatggccc tggatcggat caactagagc ttgaagtagg aggcgccttt aacgttgtac
    18241 cagacaaggc caactaccaa ggtcccctct atgaacaggt ttgtaacggt ctcaaagaag
    18301 ctggttatga ttaccaatcc actgaacaaa ccgtaacggt tctcggagtg ccaaagcatg
    18361 ctaaggatgc tagtcaaggt atcaatgctg tcatccgact agctaccatt cttgctcctc
    18421 tccaagaaca ccctgctctc agttttcttg caacacaagc aggtcaagac ggcacaggaa
    18481 gacaaatctt tggtgatata gcagatgaac cttctggtca cctatccttt aatgtcgcag
    18541 gtctcatgat caatcatgaa cgttctgaaa tccgtattga cattcggact cctgtcttag
    18601 ctgacaagga agaactagta gagttgctta caagatgtgc acaaaactac caactccgct
    18661 acgaagagtt tgactatcta gcgcctctat acgtcgcaga agacagtaaa ctcgttagca
    18721 cactgatgca aatctaccaa gaaaagactg gcgataacag tcctgctatt tcatccggtg
    18781 gtgccacttt tgctcgcacc atgccaaatt gtgtagcctt cggcgcctta ttcccaggag
    18841 cgaagcagac agaacatcag gcaaatgaat gtgccgttct agaagatttg taccgtgcta
    18901 tggatattta tgccgaagcc gtctatcgac ttgcaactta atcaggcaac tgtttctacc
    18961 aaaaaaaatc gaccgattaa tgaactgcac cccaaaagtt agacagaata aatctaactt
    19021 ttggggtgtt ttattatgaa attgagttat gaagataaag ttcagatcta tgaactaaga
    19081 aagcaaggac aaagcttcaa acagctttca aaaagatttg gtgtggatgt ttctggtcta
    19141 aagtcatctg aatctttgag atgagcttta taaatcgctt ttttcagttt ttgcactggt
    19201 gtttcgataa actcaaactt tttagccgtg gtattgcctg attttatagt atattgaaac
    19261 tagaatagta cacctctact tctaaaacat ttttagaaat cgatttgact gtcctgaacg
    19321 atttgtcctg ttcttatttc attttactat atttgagcca cttcgtcttt aacggcttta
    19381 ttcataagct cttgtaattt ttctttacta tcaattactt ctgattttcc gttgtaattt
    19441 attgtaatag gttttaactt acctaatttc tcgacacgct cattaatttg atcttttttg
    19501 aaggctgcat atgtttttcc taagattttt tcgaaaatat atttatcaga tagcggtttg
    19561 ccttcttctt cagcttggtt tttgtattga tttgaaacat aaggaacaaa tccttcatag
    19621 taacctaatg ctcccataag ttcaaaagct tgttttctaa ttcaaaccat tgcaactcag
    19681 atttcagctt ttcagataaa tcctgctcat ccaaataatg acttgaaatt agtgctgaac
    19741 tcgtttctgt atcctgtaca ggctgatcac ccataccagc aaaaaataaa ctcgttccta
    19801 gcaagaccga acaagctcct attgcatatt tcctcaaaga aaaacgctgc tttctctcaa
    19861 attgaaattc tttcatccca tctcccatca ttcattatta ctgtatattt tgtatatcag
    19921 aaatagtttg tattcacaaa tctttctagt tattccctta tcattcctaa ttaagggagg
    19981 taacatacaa taatttttag ttaaatgtat atctatgttt tttgtttttc ttaataaacg
    20041 caatacaaaa agagcctgtt gccaagctct ttgtactcaa tgaaaatcaa atagcaaact
    20101 aggaaactag ccacaggttg ctcaaaacac cgttttgagg tagtagataa gactgacgaa
    20161 gtcagctcaa aacactgttt tgaggttgca gatagaactg acaaagtcag taacatctat
    20221 acggcaaggc gacgctgacg tggtttgaag agattttcga agagtattag tctattattt
    20281 cttctcagcg cgaagggctg acaagatttg tgttcggata tcatccacac catttggcgt
    20341 atttggtaaa aagatggttt gatttccttt agaggcaaag gtattcaagg tatccaaata
    20401 ctggttggtc aagaggatag acatgatttg ttcttctgtc atgccaacat tggcttcctt
    20461 gagttcggtg atagactctg ccaatccatc cacaatcgcc ttacgttgtt gggcaatccc
    20521 cacaccatga aggcggtctt tttctgcttc tgcttcagct gcagtgacaa ttttaatctt
    20581 gtcagcttcc gccaattctt gtgctgcgac ccgcttacgt tgcgccgcat tgatttcatt
    20641 catggattgc ttaacttctg catccggttc gactttggta atcaaggttt tcacgataat
    20701 gtagccgtaa gtggtcattt cttctgctac ttggtgttga acttcaaggg caatctcatc
    20761 ttttttctca aacaattcat ccaaggttaa ttttggaaca gaagagcgaa gagcatcttc
    20821 gatataagat ttaatctgag attctggacg tatgagttta tagtaagcat ctgtcacgct
    20881 ctgctcgttg acacggtact gagtcgctac attcatcata acgaacacat tgtccttggt
    20941 cttagtctca accacaatat cactttgcaa caagcgcaac tgaatccgtg ctgcaatcga
    21001 gtcaatccca aaaggcaagc gaatatgaat accgctatta gcaacctttt ggtatttccc
    21061 aaagcgttca ataatcgcca ccgactgctg acgaaccaca taaactgtac tcagtgtgac
    21121 tatcaccaat aggagcacac aaacaatcag aaaaatcatg aaaaatattg ccataatgga
    21181 acctccacaa gtatttttct agtattatag cacatttaaa gaaggctgtg ccgtttttac
    21241 tgcgattttt cctgaaatgt caataattag aggtgaattg tcctattgtc gtccaatctc
    21301 ttgctaaaat aactctttat aaaaggcaat cgtttcttct aaggttggca taaatggatt
    21361 tcctggtgcg caggcatcaa tcaaggcatt cttagaaagg tattcaaagt cgaaatcttt
    21421 ttcttcaata ccaagttcag tcagtttctt aggaatacct actgtctcag aaagcttctc
    21481 aatctcaaca atcgcataat cggcacattc ttgatctgat ttaccttcta catgaagtcc
    21541 caaggctttg gcaacattgc ggaaagcttc tggtacacgt ttagcatttt catgttctat
    21601 aactggtagc aacatggcac agcaaacacc atgtggcaaa ttatatactg caccgagttg
    21661 gtgagccatt gagtgaacat aaccaagacc agcattatta aagctcatac caccaaggaa
    21721 gatggcattg accatacctt cacgcgcttc aatatcatgt ccattttcta cagcacgagg
    21781 aagatattcc ttgataagct caatagcacc aatagacagt ttcttggtta catcataagc
    21841 accaggtgtt accaaagctt caacagcgtg agtcagagca tccataccag tagcagcagt
    21901 caaggcttga ggtttggaaa gcatgagttc agggtcattg acagaaatca aggcaaggct
    21961 attcttgtca accattacca tcttaacctt gcgttcttca tcagtaatca catagttaat
    22021 agtgatttct gctgatgtac ctgccgttgt attgatagcc acaaccggca agcctttttt
    22081 agcagactta tgaagccctt cataatcctg tggttttcca ccatttgtag ccatgataga
    22141 aatacaacta gctgcatcct gtggagaacc cccgccaaga ctgatgataa aatcacatct
    22201 atgttcttgc aaagcagcta ccccatctgt gacattttta caagtagggt ttggctctac
    22261 atcgctaaag atgacatatt cgattccttc tgtatccagt ggttttaaag tcttaggtaa
    22321 aatatcactg ccttcgatgt acttatctgt caccaaaagt gcctttttat agccaagttc
    22381 cttgatataa ggacccactt catttacaac accttttcca ataaggttga ctgccggaac
    22441 ataaaatata gccatataat tttcctcctg cgcctccgcg ctatctattt aacatcatta
    22501 tattactttt tgtattcgtt ttcaattatt tgtgaattgt ttaacaaatc tttttaaaag
    22561 gataggaggg acgtaagttc acctcctata taaacaagtt ttatttatgt agtggtccca
    22621 acaactgaca tgcacgatag tctgctgatt ctagatcttc tgttccaaat aaggaccaat
    22681 ttttaggtct aaagatatct tcctcaggta cattatgcat attgacagga attctcaaca
    22741 tagaagccaa ggtaatcaag tctgctccaa tgtgtccata tgttatggct ccgtgattag
    22801 ctccccaatt attcatgacg tcatagacag acttgaaagc accttttcct gtcaaacgtg
    22861 gagcaaacca agtagttggc catcctgaat ctgtacgatt atctaaagta tggtgaacat
    22921 cttcaggaag ttcaagtgtg taaccttctg caatttgtag cactggacca acccctttta
    22981 gaagattgag acgtaccatt gttactggca tatccccctt cgtcaagaaa cgagttgaga
    23041 atcctcctcc acggaagtac tcgcggtttg ctggtgggaa gtctgtattt tcaagcatag
    23101 cctgtacttc actttcttcc aactcccaga atggtttcat aacaggtttg ccatctcgag
    23161 tagcttgacc tgtaccatcc aatgtacaag aaccagagtt gattagatgt aagaagccag
    23221 ctgcagcaca accctctaaa gtatgtcccg ttacacgttt aacagcctct ggactccaat
    23281 aagtgcgcac atcagcaaag atttgtggag tatttgttaa tagataatta aagagcatag
    23341 acacaccatt tagtgaatca ttctctgtcg caaatacaaa tggttttcga ataccattcc
    23401 agtcaaactg agtattgagg aaagtttcca taaaatctcc atttggaaaa tggtctgtcc
    23461 actgacgttg accttggaaa ccagctacta aagcatggtg accaaccgct tcttcctcaa
    23521 aaccaagttc agcaagcctt gggttaccaa ccattaagtc acgtccaatc atgaacatct
    23581 taataacaaa ttcccattgt ttttctttct cttctttaga aagaatcaaa tcctcacgat
    23641 ttcggtcaac cccttctttg atatgttctt ttacccaaac catagcacgt tcgaactctt
    23701 ctgggtcata aataccacgg tccatacggc gcgtgaactc cgtcatatct accgattcat
    23761 ttcgcattcc taagtattct tggaagaaat ctggatttac aatagaaccg ccaatcccca
    23821 tcgaaacact acccattgat aggtaagcag tgtctctcat caagccagtt gcaagagctg
    23881 cacgcgcata acgtaaaagt ttttctttga catcttctgg aatatctgta tcattagctt
    23941 cctgaacatc tctaccataa atcccaaagg ctggaatccc tttttgagca tgtgaagcta
    24001 gtacagctgc aagatagaca gctcctgggc gttctgtccc attaaatccc caaatagcat
    24061 gaggaatatc tggagacata tccatagttt cactaccata acaccagcat ggtgtaactg
    24121 taattgttgc gcaaacattt gattttttaa acaactcatg ggaagctgca gcctctggaa
    24181 cacgaccaat ggtagatgga gaaatcacac attccacagg ttccccatct ggatatttca
    24241 atgtgcttga aatcaaatct gccacacttt tagccatgtt cattgtttgt acttcaagtg
    24301 attcgcgtac accttgacga cgaccatcaa tagtcggacg aatcccaata cgtggatgtt
    24361 gaatcatact attttcctct tatttttctg tttctttcat actaccgatg ataattttta
    24421 aaattactta taagtataaa cctctacctc tgccaaactt aaaggaattc ctgcgtttct
    24481 caaagaaatt ctaacctttt ttcccttcag cccttttaag tctatggata gattattgct
    24541 ttctaaactg tcaatatgtt gtttaaaaac ttcatgatta tttgaatcat atataataat
    24601 atcaaaattt gataatctct gaatttcagc atctgttcta ttataaataa ttaacttttc
    24661 taattcttcg gtttgtgcta aatctatctc ccaccatgaa ggagaatctc ctttggtatg
    24721 agtcactgaa tgatgaccat aatctccgtt tttatttcca tcaactgcta gacgagaaaa
    24781 accattgtaa tccgttgaac tctgtcgagt ttctttggtt aatgcaatat tagaaagttt
    24841 cttcggagtt ctctttgaac cataggcttc aacttccgct aaacttaacg gaatagctgc
    24901 tgattttaat tctattttga ctagcttagc ccctacagaa ggtactttta aagataacaa
    24961 accatcaact actttatcaa aatgtcttct aaaaacttct tctcctgatg aagatagaaa
    25021 aataacatca aaattagata aacgctgagg ttcggcatct gttctattat aaatatcaac
    25081 tttagaaacc gtaaactctt ctcccagatc gacctgccac caagcgttag aatctgcctt
    25141 agtatgagtc accgaatgat gtccgtaatc tccatcttta ttaccatcaa ctgctaaagc
    25201 agctacaccc tcataattgg ttgaactttg agtagctacc ttatttgtag agactacttt
    25261 atcttctgtt attttatcta tatcctcttc agattgctta ccatctgatt ctctaaaaac
    25321 ctctacttct gctaaactca aaggcactcc actcgttagt aatttaactt taatatacct
    25381 tgctcctttt tctttgaaat ctagactaac agattcaccc gacaaattat taacatgttt
    25441 cttagcaact tcgtttctat tattgtcata tagaatcaca tcaaaattag atagacgttg
    25501 agtctcagca tctgtgcgat tataaatttt aacaagccca actttatcca tttttttcaa
    25561 atcgacttcc caccaagagg gattatctgc cctagtgtgt gttaccgaac cagagttaaa
    25621 attaccattt ctgttaccat ccacagctct attagggtct ccattgtaat ctacagaact
    25681 ttgtgttgtt ggttttgcat atgctatatt caaattacca tcattaaatt tatctggtgt
    25741 tgggaaaggg actgggcctg tcccttcagt atttatagac atctctacca ttccattatg
    25801 attaaccgta atggtataaa catgggtatt ctcatcccaa ttttctgtat aagtataatg
    25861 atttttatcg ccacttatat ttatctgagg tttatgacca gtatgccctt ttaatgtaag
    25921 tgttgttgtc ctaaagtcat tatctacagg attgatggaa taatttttgc ttatccagtt
    25981 cgctaactct aattcttctt tcttccaact ttttgatgca tcaaaatttc ctgataatgc
    26041 ccacatagct gtcttatctg tcctgtaatt
//
LOCUS       DAVBBS010000027        25336 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVBBS010000027 DAVBBS010000000
VERSION     DAVBBS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25336
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.027"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..309)
                     /locus_tag="VQU32_001091"
     CDS             complement(<1..309)
                     /locus_tag="VQU32_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HET2472554.1"
                     /translation="MKKKWMYYAACSSNESADDSSSDKGDGGSLVVYSPNSEGLIGAT
                     IPAFEEKYGIKIELIQAGTGELFKKLESEKEVPVADIIFGGSYTQYATHGELFENYIS
                     K"
     gene            complement(897..1673)
                     /locus_tag="VQU32_001092"
     CDS             complement(897..1673)
                     /locus_tag="VQU32_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HET2472555.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
     gene            1795..2541
                     /locus_tag="VQU32_001093"
     CDS             1795..2541
                     /locus_tag="VQU32_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2472556.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            2554..3534
                     /locus_tag="VQU32_001094"
     CDS             2554..3534
                     /locus_tag="VQU32_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HET2472557.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            3729..4049
                     /locus_tag="VQU32_001095"
     CDS             3729..4049
                     /locus_tag="VQU32_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2472558.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            4095..4403
                     /locus_tag="VQU32_001096"
     CDS             4095..4403
                     /locus_tag="VQU32_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2472559.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            4420..5718
                     /locus_tag="VQU32_001097"
     CDS             4420..5718
                     /locus_tag="VQU32_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2472560.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQTLGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
     gene            5869..8307
                     /locus_tag="VQU32_001098"
     CDS             5869..8307
                     /locus_tag="VQU32_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HET2472561.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            8326..8994
                     /locus_tag="VQU32_001099"
     CDS             8326..8994
                     /locus_tag="VQU32_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HET2472562.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEVGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            9012..10100
                     /locus_tag="VQU32_001100"
     CDS             9012..10100
                     /locus_tag="VQU32_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HET2472563.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            10555..13056
                     /locus_tag="VQU32_001101"
     CDS             10555..13056
                     /locus_tag="VQU32_001101"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET2472564.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSTEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLIRSKEIVAENNGALD
                     KVYNETVKAVTEQIDSLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAATGESISYVTWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            13250..13945
                     /locus_tag="VQU32_001102"
     CDS             13250..13945
                     /locus_tag="VQU32_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2472565.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPQEMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYESLREL"
     gene            13957..14373
                     /locus_tag="VQU32_001103"
     CDS             13957..14373
                     /locus_tag="VQU32_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2472566.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(15000..15092)
                     /locus_tag="VQU32_001104"
     CDS             complement(15000..15092)
                     /locus_tag="VQU32_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HET2472567.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            15253..16224
                     /gene="ruvB"
                     /locus_tag="VQU32_001105"
     CDS             15253..16224
                     /gene="ruvB"
                     /locus_tag="VQU32_001105"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET2472568.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            16205..16777
                     /locus_tag="VQU32_001106"
     CDS             16205..16777
                     /locus_tag="VQU32_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET2472569.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTAVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            17008..17766
                     /locus_tag="VQU32_001107"
     CDS             17008..17766
                     /locus_tag="VQU32_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET2472570.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            17775..18578
                     /locus_tag="VQU32_001108"
     CDS             17775..18578
                     /locus_tag="VQU32_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET2472571.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            18600..19859
                     /gene="rseP"
                     /locus_tag="VQU32_001109"
     CDS             18600..19859
                     /gene="rseP"
                     /locus_tag="VQU32_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET2472572.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            19872..21725
                     /locus_tag="VQU32_001110"
     CDS             19872..21725
                     /locus_tag="VQU32_001110"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET2472573.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            21825..23204
                     /gene="bglA"
                     /locus_tag="VQU32_001111"
     CDS             21825..23204
                     /gene="bglA"
                     /locus_tag="VQU32_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET2472574.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNELIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIHDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KKLSDNNGF"
     gene            23396..25204
                     /gene="glmS"
                     /locus_tag="VQU32_001112"
     CDS             23396..25204
                     /gene="glmS"
                     /locus_tag="VQU32_001112"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET2472575.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
BASE COUNT         7735 a         4313 c         5537 g         7751 t
ORIGIN      
        1 ttttgaaata tagttttcaa agagttctcc gtgggtagca tattgtgtat aagaaccacc
       61 aaagataata tcagctacag gaacttcttt ttctgactct agttttttga aaagttctcc
      121 agtaccagct tgaatcagtt ctattttgat accatatttt tcttcaaagg caggaatagt
      181 tgctccaatt aagccttctg agtttggtga ataaacgact agcgaaccac cgtctccttt
      241 atcagatgaa ctgtcatcgg cagattcatt agaagaacaa gcagcataat acatccattt
      301 ctttttcatg atggatacct ccgttgtgtt atttaagttt attttaaaac aatgtaagcg
      361 tttttaaaac ctacaattct attctatagt gtattgaatc tataacagta cacattgact
      421 gctaaaatat ttctataaat taatttgact ttcctgatag agatgttcac atcttatttc
      481 aattcactat attagagtaa aattctctac aaaaaaaaag aatagcctat tttactattt
      541 ttccgagtga tttcaattcc tttggggaaa tatggagata ctttttaaat cctgacaaat
      601 ggttgtttct ttttctaaat cggtgatact gtatcggaga atgcgcgtga ggtcacaaag
      661 gctgcgatag agcttctatg gagaatttct ttttggagag attttttaaa ggaatgagac
      721 atccgctacc tccttggaag gtttttttgt gataagtata gcagtttaaa aattctagag
      781 caaggtaaaa tagaaaaggt ttggctaaaa ttttttagct aaaccttttg aaaagttaag
      841 tatgtattat agatgcttgt tatctgcaaa tagctagagc atttcaagga aataaactag
      901 aaataacaat taatatggtg gttcagaaat accttttgat aatcaataag atcttcagga
      961 tgaagtgcgt tgattccatc catttcatat ttccgattta ataaacgata tttgttttca
     1021 ccaaattgat gaaaagggag tagttgaact tggtcgatat ttaatgagtt aaatagagta
     1081 gcgaattttt ctgcatcctc taaactattg ttaaaattag gaataactgg gattcttaaa
     1141 acgatagttt tattttgtga aaaagcataa tgaatgtttt taataatcat ttgattaaaa
     1201 accccagtca cttttttatg ttttatagaa ttataatgtt ttaggtctgt gtagataaaa
     1261 tccacatatt gaattaaatc aataaatttt tcatgatcaa caaaggcagt agtttcaatg
     1321 gcagtgtgta tgtgatgttc tttagctgat tttaagatag ctttagcaaa ttcaaactga
     1381 gcaaatattt cacctcctga taaagttaaa cctccaccgg attcttcgta aaattctttg
     1441 tcttttaata cctctgtaat aatttcttct acagtctttt cttcaccgac taaggtgaat
     1501 ttctctcgtt gagcatcttt cattttttca ggtttcattc tttgagattc aggattagaa
     1561 caccatggac agcgcagagg acatcctttt aaaaaaacag ttgtacgaat acccgggccg
     1621 tcatgaattg aaaagtgttg aatattaaaa ataattcctt tagatatttc cataagagtt
     1681 cctcctcttc ataagtctat cctatcacaa aaacgaaaga aaaacaatta cgaacgaaag
     1741 gttaatttgt tgttttttgc tttttatttt ggtagaataa aacggaggtg taaaatgaaa
     1801 agattggaac aaattattaa attagtatca gaatatgaaa agatcgacgt taatacatta
     1861 tcggaaaaat taaatgtatc gaaagtaacg attagaaaag atttagataa attagagtca
     1921 aaaggtttat tacacagaga gcatggatat gctgtattaa atagtggaga tgacttaaat
     1981 gtacgtttgt caattaatta tgaaattaag agaaaaattg ttcaggaagc agtaaaattg
     2041 gtgtcagata atgaaacgat aatgatagaa tctggatcga cctgtgcttt acttgctgag
     2101 gaaatttgca agcaaaaaag aaatgttacg attgtaacaa attcattttt tatagcaaat
     2161 tttgtgagag cttatgattc atgtcgtatt attgttcttg gtggagagtt tcagaaggat
     2221 tcacaggtta ctgtgggacc tttattaaaa gaaatgatac agacttttca tgtgcatcaa
     2281 gcttttgttg ggacagatgg atacgataaa gagatgggct ttacaggaaa agatttaatg
     2341 cgcagtgagg tagttcaata tatttcagca gcgtcggata aagtcattgt cctaactgac
     2401 tcaagtaaat ttgataaaag gggtacagta agaagatttg ctttaagtca agtctatgaa
     2461 gtaataacag acgaaaaact ttctaaacaa aatatagcta cattagaaaa tgctgggata
     2521 atggttaagg tagtttcgta agaggttaag tgtatgaatc aagataggaa taaactgctc
     2581 tctaaaattg cttatctgta ttatattgaa aacttaaatc agtcacaaat agcagcaaaa
     2641 ttaggaattt atagaacctc tattagtaga atgttaacag aagcaaggaa tgcaggaatt
     2701 gttaaaattg aaatagagaa ttttgatacc aatatgttta agttggaaaa ttatgtaaaa
     2761 gaaaaatatg gtctggaaag tttagaaatt attccaaatg aatttgatga taatccaaca
     2821 attttatctg aaagaatttc tcaagttgca gcaggtgtcc ttaggaatct aattgatgat
     2881 aatatgaaaa ttggcttttc ttgggggaaa agtttaagta atttagtaga tttaattcac
     2941 agtaaaagtg tccgaaatgt tcacttctat cctctagcag gtggtcctag tcacatacac
     3001 gctaaatacc atgtgaatac actgatttat gaaatgtcta gaaaatttca tggagagtgt
     3061 acatttatga atgcaacgat tgtgcaagaa aataaattgt tagcagatgg tattttgcaa
     3121 tcaagatatt ttgaaaattt gaaaaatagt tggaaagatt tagatatagc tgtagtcgga
     3181 attggtgatt ttagcaataa aggaaagcat caatggttag acatgcttac agaggatgac
     3241 tttaaagaat tgaccaaagt taaaactgta ggagaaattt gttgtcgatt ttttgattca
     3301 aaaggtaaag aagtctatga aaacttacag gaaagaacga tagcaatctc tttggaagat
     3361 ttaaaaaata ttcctcaaag tttagctgtt gcttacggtg atacgaaagt atcttcgatt
     3421 ctttctgtct tgcgtgctaa tttagtaaat catttgatta cagacaaaaa tacaatttta
     3481 aaagttttgg aagaagatgg ggatttgact tttagagaga ttctaggtga gtgaaaatga
     3541 tagactgatt cagtttatcg tttttctttt tagttgattg cacatttgtg cttatataaa
     3601 caaaaatagt ttatctgttg tttttggatt gacaacttta ttatgtagtt gtattctata
     3661 gttacaaaag aaaattttaa aatttcaaat gaaaaaagct ttttacatag tgaaatgagg
     3721 aggaatttat ggaaattatt gttccagatc aaattatcat gggtttaatt ttatatgctg
     3781 gtgatgcgaa acaacatatt tataaagcgt tagattacat aaaaaatggt acatgtgaac
     3841 ggtgtgaaga agaaatccag ttagctgatg ccgccttatt agaagcccat aatctacaaa
     3901 caaaattctt ggcacaagaa gcgtctggta caaagacaga aattacagct ctctttgttc
     3961 attcacaaga tcatctcatg actagtatga cggagattaa tttaatcaaa gaaattatta
     4021 gtttgagaaa agaacttcat aaaaaataat actagagtat tatcattgtt attaacatag
     4081 aggaggaaaa cataatggtg aagattggtt tgttttgtgc agcaggtttt tctactggta
     4141 tgcttgtaaa taatatgaaa attgcagcgc aatctagtgg agttgaggca gaaatagagg
     4201 cgttttctca gtctaaatta gcggattatg cgccaaatat agatgttgca ctattgggtc
     4261 cacaagttgc ttatacatta gataaatcaa aagaaatttg tgataagtgt gatgttccga
     4321 tagctgttat tccgatgatg gactatggta tgttagatgg gaaaaaagta ttagatttgg
     4381 ccctatcttt gattagtggg taagaaaagg agatttatta tgtcaaagat ggatgttcag
     4441 aaaatcattg caccgatgat gaagtttgtg aatatgcgtg gcattatagc tctaaaagat
     4501 gggatgttag caattttgcc attgacagta gttggtagtt tgttcttgat tatgggacaa
     4561 ttgccgttcg aaggattaaa taagagcatt gctagtgttt ttggagctaa ttggacagag
     4621 ccgtttatgc aagtatattc aggaactttt gctattatgg gtctaatttc ttgtttttca
     4681 attgcctatt cttatgctaa gaatagcgga gtagaggctt taccagctgg agttctatct
     4741 gtatctgcat tctttatttt gctaagatca tcttatatcc ctaaacaagg tgaggcgatt
     4801 ggggacgcta ttagtaaagt ttggtttgga ggccaaggaa ttatcggtgc tatcattata
     4861 ggtttggtag taggaagtat ttataccttc tttataaaga gaaaaattgt tattaagatg
     4921 ccagaacaag ttccacaagc tattgccaaa cagtttgaag caatgattcc agcatttgta
     4981 attttcttat cttctatgat tgtatatatt ttagcgaagt cattgactaa tggcggaaca
     5041 ttcatagaaa tgatttattc tgctattcaa gttccgttgc aaggtttaac tggatctttg
     5101 tatggtgcta ttggaattgc attctttata tcatttttgt ggtggtttgg tgttcatggg
     5161 caatcggtag taaatggagt agtgacagct ctgcttttat ctaatcttga cgctaataaa
     5221 gctatgttag cctctgctaa tctatcatta gaaaatggtg cacatattgt tactcaacaa
     5281 tttttagatt catttttaat tctatcaggt tcagggatta cgtttggtct tgtagttgcc
     5341 atgctttttg cagcaaaatc aaaacaatac caaaccttag gaaaagttgc agcttttcca
     5401 gcaatattta acgtaaatga gccagttgta tttggatttc cgattgtcat gaatccagtt
     5461 atgtttgtac ctttcattct tgttcctgta cttgcagctg tgatagtata tggagctatt
     5521 gcaacaggtt tcatgcagcc attctcaggg gtaacattgc cttggagtac accagctatt
     5581 ttatcaggat ttttggtggg tggatggcaa ggagttatta ctcagctggt gatattagcg
     5641 atgtctacat tggtttattt tccattcttt aaagtacagg atcgtttagc ttaccaaaat
     5701 gaaatcaaac aatcttagag gtatttgtgt gttactgtta aactcacaca tttgtgctaa
     5761 aaattagaga gttaaaattt ttctagttaa aagcttgaaa atttctataa aaatcggtat
     5821 tatattttcg aaagaaataa aaatattttc gaaagaaagg tggttacgat ggtaaataca
     5881 gaagtagcaa gaacaacaat caagacagaa tattttggca gccttactga aaggatgaac
     5941 aaatatcgag aagatgtttt aaataaaaaa ccttatattg atgctgagag agcagttcta
     6001 gcaacacgcg cctatgaacg atacaaggaa caacctaatg tcctaaaacg tgcatatatg
     6061 ctgaaagaaa ttttggaaaa tatgactatc tatattgaag aagaatctat gattgcggga
     6121 aatcaagctt cttccaataa agatgctcct atttttccgg aatatacgct agaatttgtt
     6181 ctcaatgagt tggatctttt tgaaaagcgt gatggagatg ttttctatat tacagaagaa
     6241 acaaaagaac aacttagaag tattgctccg ttttgggaaa ataataattt acgtgctaga
     6301 gctggtgcct tattacctga agaagtgtct gtttatatgg aaacaggatt cttcggtatg
     6361 gaaggtaaga tgaattctgg agatgctcac ctagcagtta actatcagaa acttttgcaa
     6421 tttggtttaa gaggttttga agagcgggct cgtaaagcaa aagtagctct agatttaaca
     6481 gatccagcaa gtattgataa atatcatttt tacgactcta tatttatcgt aatcgatgct
     6541 attaaagtat atgcaaagcg ctttgttgct cttgctaaaa gtttagccga aaatgcaaat
     6601 cctaaacgta agaaagaatt acttgagatt gcagatattt gctctagagt cccatatgaa
     6661 ccggcaacta cttttgcaga agctattcaa tcagtttggt ttattcaatg tattttacaa
     6721 attgaatcta atggccactc tctttcatat ggccgttttg atcaatatat gtatccatat
     6781 atgaaggctg atttagaaag tggtaaagaa acagaagata gcattgttga acgtctgaca
     6841 aatctttgga ttaagacaat tacaatcaat aaggttcgca gtcaatcaca tacattttct
     6901 tcagcaggaa gtcctttata tcaaaatgtt acaattggtg gacagactcg agataagaag
     6961 gatgctgtta acccattatc ttatttggta ttaaaatcag ttgcacaaac ccatctaccg
     7021 caacctaatc taactgtacg ttaccatgca ggtttagatg ctcgtttcat gaatgagtgt
     7081 attgaagtga tgaaacttgg ttttggtatg cctgcattta ataatgatga gattattatt
     7141 ccttctttta ttgcaaaagg agtattggaa gatgatgctt atgattacag tgccattgga
     7201 tgtgttgaaa cggcagttcc agggaaatgg ggctatcgtt gcacaggtat gagttatatg
     7261 aacttcccta aggttctact tatcacgatg aatgatggaa ttgatccggc ttcgggtaaa
     7321 cggtttgcac caagctttgg tcattttaag gatatgaaga acttttctga attagaaaat
     7381 gcttgggata aaacactaag atatttgaca cgaatgagtg ttattgttga aaattctatt
     7441 gatttatcat tggaacgaga agttcctgat attctatgtt cagcattgac tgatgattgt
     7501 attggtcgtg gaaaacacct taaagaaggt ggagcagtat atgattatat atcaggattg
     7561 caagttggaa ttgcaaattt gtcggattca ttagctgcaa ttaaaaaatt ggtgtttgag
     7621 gaagaacgta taagcccaag tcagctttgg catgcactgg aaacagatta tgccggagaa
     7681 gaaggtaagg tcattcaaga aatgttgatt catgatgcac ctaagtatgg taatgatgat
     7741 gattatgctg acaaattggt tactgctgct tatgacattt atgttgatga aattgctaaa
     7801 tatcctaata cacgttatgg aagagggcct attggaggaa ttcgttattc aggaacatct
     7861 tctatctcag ccaacgtagg gcagggacgt ggaacattag caactccaga tggacgcaac
     7921 gcgggtacac cgttagcaga gggttgttca ccatcacata atatggatca acacggccct
     7981 acatctgttt taaaatctgt ttcaaaatta ccaacagatg aaatcgtagg tggggttctc
     8041 ttaaatcaga aagtaaatcc tcaaacgtta gccaaagaag aagataaatt aaaactaatt
     8101 gctttgttac gaacattctt taatcgttta catgggtacc atattcaata caatgttgtt
     8161 tccagagaga cgttgattga cgctcagaaa catcctgaaa aacacagaga cttaattgtt
     8221 cgtgttgcag gatactctgc attcttcaat gttctttcta aggcaaccca agatgacatt
     8281 ataggacgta ctgagcatac tttgtaaaat aaagaggttc tttttatgga atttatgctt
     8341 gacacattaa atttagatga gattaaaaag tggtctgaaa ttttgccgct agctggggta
     8401 acttcaaatc ccactattgc aaaaagagag ggttctatta atttttttga acgaatcaaa
     8461 gatgtaagag aattgattgg ctctacaccc tctattcatg ttcaggtgat ttctcaagat
     8521 tttgaaggca tcttaaagga tgctcatgaa attcgaagac aagcaggaga tgatatattt
     8581 atcaaagtac ctgttactcc agctggatta cgtgcaataa aggtgctaaa aaaagagggc
     8641 taccatatca ctgcaacagc tatttataca gttattcagg gattattagc tatcgaagta
     8701 ggagcggatt acctagctcc atattataat agaatggaaa atctgaacat tgattcaaat
     8761 tctgtcattc gtcaattagc tcttgctatt gatagacaga actctcctag taagatttta
     8821 gctgcatcct ttaaaaatgt agcacaagta aataatgctt tagctgcagg tgcgcatgct
     8881 gttacagcag gagcagatgt ttttgaatca gctttcgcca tgccatctat ccaaaaggcg
     8941 gttgatgatt tttctgacga ttggtttgtt actcaaaata gtcgttccat ttagatagag
     9001 aggaaataca tatgagaatt tttgctagtc cttctagata tattcagggg gaaaatgcct
     9061 tgtttgaaaa tgccaaatca attttggatt tgggaaatta ccctattcta ttatgcgatc
     9121 agttggttta tgatattgtt ggaaaacgat ttgaagatta tctacatagg tatggtttcc
     9181 atattgttct ggcgctattt aatggtgaag cttctgacaa tgaaatcaat cgagttgttg
     9241 ccttggctga gaaagaaaat tgtgatagta ttatcggtct tggtggggga aagacgattg
     9301 atagcgcaaa agctattgca gatttgattg aaaagcctgt tattattgct ccaacaattg
     9361 catcgaccga cgcacctgta tctgctttat ctgttattta tacagatgaa ggtgcatttg
     9421 atcattatct attttattct aaaaatccag atttagtttt ggttgataca aaagttattt
     9481 cacaagcccc taagcgttta ttagcgtctg gtattgcaga tggtttagca acttgggttg
     9541 aggcgcgtgc ggttatgcag gcaaatggaa aaactatgtt gggacaacag caaacattgg
     9601 ctggagttgc aattgcgaag aaatgtgaag aaacgctgtt tgcagatggt ttacaggcta
     9661 tggcagcttg tgaagctaaa gtggtgacac cagcattaga aaatattgtt gaagctaata
     9721 ctttattgag tggcctaggt tttgaaagtg gaggattagc tgcggcgcat gcaattcata
     9781 atggttttac tgcattgaca ggtgacattc atcatttaac acatggtgaa aaagtagctt
     9841 atggaacttt agtacaacta ttattggaaa atagacctaa agaagaactt gataagtata
     9901 ttgagtttta caaaaaaatt ggtatgccaa caactctaaa agaaatgcat ttggatcaag
     9961 ttggatatga tgatttaata aaagttggta aacaagcaac tatggagggt gagacaattc
    10021 atcagatgcc gtttaagatt tcgccttcag atgttgctca agctattatc gctgtagatg
    10081 cctatgtaaa ttcaaaataa acaataagga ctactgtttt ccaaatggta gtcttttatt
    10141 gatcctcgta ttgaattcta tagcagattg aaataagatg agaacaaatc gattgggaaa
    10201 gtaaaattaa tttctataag tattttagca attgtttcgt actatttcag attcagtcca
    10261 ctatacgttc ttcataaatc aaaaagcgac ataggttgtc ggctatttat tgtgaataca
    10321 ttaattagca ttccagtttt atcttcggtc taaaataagt attttgtgct atacgagata
    10381 agtttcttga cttactcctt gatttactgc ataacaatgg gataaaaagt gggagataga
    10441 gcaattcata gtcatcaaaa ttaatgagat acagtataca gtttttcctt taaacacatt
    10501 tcaaattccc tcaaaaatgg tataatagta acatcacaaa attggagaga gaccatgagt
    10561 ttttacaatc ataaagaaat tgagcctaag tggcagggct actgggcaga acatcataca
    10621 tttaagacag gaacagatgc atcaaaacct aagttttatg cgcttgatat gttcccttat
    10681 ccgtctggag ctggtctgca cgtaggacac ccagaaggtt atactgcaac cgatatcctc
    10741 agtcgttaca aacgtgcgca aggctacaat gtccttcacc caatgggttg ggatgctttt
    10801 ggtttgcctg cagagcaata cgctatggat actggtaatg acccagcaga atttacagcg
    10861 gaaaacattg ccaacttcaa acgtcaaatc aatgcgcttg gattttctta tgactgggat
    10921 cgtgaagtca acacaacaga tccaaactac tacaagtgga ctcaatggat tttcaccaag
    10981 ctttacgaaa aaggcttggc ctatgaagct gaagtgccag taaactgggt tgaggaattg
    11041 ggaactgcca ttgccaatga agaagtgctt cctgacggaa cttctgaacg tggaggctat
    11101 ccagttgtcc gcaaaccaat gcgccaatgg atgctcaaaa tcacggctta cgcagagcgc
    11161 ttgctcaatg acttagatga actagattgg tcagagtcta tcaaggatat gcaacgcaac
    11221 tggattggta aatcaactgg tgccaatgta actttcaaag taaaaggaac agacaaggaa
    11281 tttacagtct ttactactcg tccggacaca cttttcggtg cgactttcac tgttttggct
    11341 cctgaacatg aattagtaga tgccatcaca agcacagagc aagcagaagc agtagcagac
    11401 tacaaacacc aagccagcct taagtcagac ttggctcgta ccgaccttgc caaagaaaaa
    11461 actggtgtat ggactggtgc ttatgccatc aatcctgtca atggtaagga aattccaatc
    11521 tggattgcgg attacgttct tgctagctat ggtacaggtg cggtaatggc tgtgcctgcc
    11581 cacgatcaac gtgactggga atttgccaaa caatttgacc ttccaatcgt cgaagtactt
    11641 gaaggtggaa atgttgaaga agctgcctac acagaagatg gccttcacgt taattcagac
    11701 ttcctagatg gactcaacaa agaagacgct attgccaaga ttgtggcttg gttggaagaa
    11761 aaaggctgtg gacaagagaa ggtaacctac cgtctccgcg actggctctt tagccgtcaa
    11821 cgttactggg gtgagccaat cccaatcatt cattgggaag atggaacttc aacagctgtc
    11881 cctgaaagtg aattgccact tgttttgcct gtaaccaagg atatccgtcc ctcaggtact
    11941 ggtgaaagtc cactagctaa cttgacagac tggcttgaag tgactcgtga agatggtgtc
    12001 aaaggtcgtc gtgaaacaaa tactatgcca caatgggcag gttcaagctg gtactacctc
    12061 cgctatattg acccacacaa tactgagaaa ttggctgacg aggatctgct caaacaatgg
    12121 ttgccagtag atatctacgt gggtggtgcg gaacatgctg tacttcactt gctttacgct
    12181 cgtttctggc ataaattcct ctatgacctc ggtgttgttc caactaagga accattccaa
    12241 aaactcttta accaaggaat gattttggga acaagctatc gtgaccaccg tggggctctt
    12301 gtggcgactg acaaggttga aaaacgtgat ggttctttct tccatgtgga aacaggggaa
    12361 gagttggagc aagcgccagc caagatgtct aaatcgctca agaacgttgt caacccagac
    12421 gatgtggtgg aacaatacgg tgctgatacc cttcgtgtct atgaaatgtt catgggacca
    12481 ctcgatgctt cgattgcttg gtcagaagaa ggtctggaag gaagtcgtaa gttccttgac
    12541 cgtgtttacc gtttgattag aagtaaggaa attgttgcgg aaaacaatgg cgctcttgac
    12601 aaggtttaca acgaaactgt taaagctgtc acagagcaaa ttgattccct caaattcaac
    12661 acagccattg cccaacttat ggtctttgtc aatgcggcta acaaggaaga caagctctat
    12721 gttgactatg ccaaaggatt tatccaattg attgccccat ttgcacctca cttggcagaa
    12781 gaactctggc aaactgttgc tgcaacaggt gagtcaatct cttacgtgac ttggccaaca
    12841 tgggacgaaa gtaaactggt tgaagacgaa atcgaaatcg ttgtccaaat caaaggtaaa
    12901 gtccgtgcca aactcatggt cgctaaagac ctatcacgtg aagaattgca agaaattgct
    12961 ctagcggatg aaaaagtcaa agcagaaatt gacggtaagg aaatcgtgaa agtgattagt
    13021 gtaccaaata aattggttaa tattgttgta aaataaccaa tttatttgct gatttccaac
    13081 ctcaaacagt ttcccaaact gtttgagcca actaaactcg ttaatatcgt tgtgaaataa
    13141 ggtaggaatc cttcagagta gaatctggag gattttttga atcttcttat gaaagtatgg
    13201 tatactatgg gcaactataa agtttgaaaa gtgaaaaaag gagaaagcta tgccagtaaa
    13261 tgaatatggt caaatgattg gggagtcaat ggaaggttat acaccaggtg aactgccttc
    13321 tattgatttc ttagaagggc gttatgctag gatagaggct ctttcagtgg aaaagcatgc
    13381 ggaggatttg ctagctgttt atgggcctga tacgcctcag gagatgtgga cctacctttt
    13441 tcaggagcca gtagcagata tgggggagct ggttagcctt ttacatcaga tgttggctcg
    13501 taaggaccgt ttttactatg caatcataga caaggcgact ggtaaggctt tgggatcttt
    13561 ttctctcatg cgcattgacc agaataaccg agtaatagaa gtcggagcag tcactttttc
    13621 tccagagcta aggggtacac ggataggaac tgaagctcag tatctcctag ctcgctatgt
    13681 ttttgaggag ctaaactatc gtcgctacga gtggaaatgc gatgccttaa atctgccatc
    13741 cagacgagct gcggagcgtt tgagctttat ttatgaagga accttccgtc aggcagtggt
    13801 ttataaggga cgtacgagag atacggattg gttgtctatg attgataagg actggcctca
    13861 agtcaaagat cgtttggaaa catggttgcg tcctgaaaat tttgataaaa atggacagca
    13921 gtacgagagc ttgagagaac tttaagaggt gttgagatga ttactattaa aaagcaagaa
    13981 attgtcaagc tagaggatgt tttacatctc tatcaggctg tcggttggac aaattatacc
    14041 catcaaccag agatgctgga gcaggcctta tctcattcat tagtaattta tctggcactt
    14101 gatggtgatg ctgtggtggg cttgattcgt ttggttggag atggattctc atcagtattg
    14161 gttcaggatt taatcgtttt accaatctat cagcgtcaag gaattggtag tgccttgatg
    14221 aaagaggctt tagaggatta caaagatgcc tatcaagtcc agctggtgac agaagagaca
    14281 gaaagaacct tgggcttcta tcgttctatg ggctttgaaa tcttatccac ctataattgt
    14341 atagggatga cttggatgaa tcgtaaaaaa taacaaaact tgttttttct taagcaaaat
    14401 ttaaggatgg tctagtatta tatagtcatt aaataaagac ctcctaactt tatttaataa
    14461 aatcctaaac ttttcttttt cataataatc tcccttaact ccacccaatc aggtggagtt
    14521 ttttagctct atttcaggct tttggggact attctaaaaa taatttttcg atatttttcg
    14581 gtatttttcg gattttggtc ggggaattgg cggggacttt tttagcgaat ataactaaga
    14641 aataggtctg tggtcgcttc agctagttca aactcaattt gattgtaagt gactgttaat
    14701 tgaagaactg gaactactgt aataatgatt ttgcttgtct agtagtggcg tattaaatga
    14761 gaagtgacat gtaagattgt ctcttcgttt acgaaatcaa aatttctatg aaattgatag
    14821 aatccctaac gttctcttaa tcttaaccgt cttgtcgtac agcggttgaa gaaaatgtaa
    14881 acctttgaca aaattcgtga actatgggat aataaaaagg aattgagtac tagttctata
    14941 tcataggcta gaaaagaccc caagctcacg accaaataag cttgaggtct tttttgacac
    15001 tatttgtcct tgtttagcca tttatcgact atacgaagaa cgacaccgac cacaattggt
    15061 ccgatgatag ttttgagtac taattccatc atgggctatc tcacctcctt tcgtaggcgg
    15121 tgtagcagtg tcgtagaata ttataccaca taagtgctaa acttaggagt cacccaatca
    15181 ggtggctttt ttgtttgtgg cttggttttt tgatataata gagccatgag tagaatttta
    15241 gataatgaga taatggggga tgaggagtta gtagaacgca cgctccgtcc tcagtattta
    15301 cgtgaatata tcggacagga taaggtcaag gaccagctac aaatctttat tgaagctgcc
    15361 aaaatgcggg atgaagcgct ggatcatgtg ctcttatttg ggcctccagg tctcgggaaa
    15421 acgaccatgg cctttgttat tgccaacgaa ctgggagtca atcttaagca gacgtcgggt
    15481 ccagtcattg aaaaagccgg agatctggta gctattttga atgagttaga gcctggggat
    15541 gtacttttta ttgatgagat ccatcgtttg ccaatgtcag tggaagaggt gctttatagt
    15601 gctatggagg acttctacat agatattatg attggggctg gtgagggtag tcgtagtgtt
    15661 catttggagt taccaccttt taccttgatt ggtgcgacga ctcgggctgg tatgctctcc
    15721 aatccgctac gggcacgttt tgggattaca ggccatatgg agtattatgc ccatgctgac
    15781 ttgacagaaa ttgtcgagcg gacggcagat atttttgaga tggaaatcac tcatgaggca
    15841 gcatctgagt tggccctacg tagtcgtggg acccctcgta ttgccaatcg tctcctcaag
    15901 cgcgtgcgcg attttgccca gataatgggg aatggggtaa ttgatgatat tattaccgat
    15961 aaggctttga ctatgctgga tgttgaccat gaaggtttgg actatgtgga tcaaaaaatc
    16021 cttcgtacca tgattgagat gtacagtgga ggacctgttg gtctaggaac tctttctgtg
    16081 aatatcgccg aagagcgtga gacagttgaa gatatgtatg agccttactt gattcaaaaa
    16141 ggttttatca tgcggacacg gtctggacgg gtggcgactg ctaaggcata tgagcactta
    16201 ggttatgaat acagtgaaaa ataagcaaga aatcttagag gcttttagag aaaatccaga
    16261 tatgatggcc attctgacga tcatccgaga ccttggtctg aaagactcgt ggttggcagc
    16321 aggttctgtc agaaatttca tttggaatct tttgtcagac aaatcccctt ttgatcatga
    16381 aacagatata gatgtgattt tctttgatcc agatttttct tatgaggaaa ccttattact
    16441 ggagaaaaag ctgagagagg attttcctca gtaccagtgg gaattgaaaa atcaggtcta
    16501 tatgcatcag cacagtcctc acactgcttc ctataccagt tctcgtgatg ctatgagtaa
    16561 gtatccagaa cggtgtacgg cagttggact gcgcttgaat gaagaatcag attttgaact
    16621 ctatgcacct tatggtttgg aggatatttt gaattttcaa gttcgtccaa ctcctcattt
    16681 cttagaaaat gaagaccgaa tggaactcta tcaaacacgt ttatccaaga aaaattggca
    16741 ggagaaatgg aaaaatttga tttttaaaaa tacttaagga aactttaagc tagggagtgt
    16801 acactaagtt cataagttaa gaagacctta acttaaactc ctaaaacttt ttcataataa
    16861 tctccctata aaaataaagt cgcccaatca ggcggcttat ttttttgaaa aatgggcatg
    16921 gtgcctgaga ataaatagct tagtgataga agaaaatggg gaaatatggt ataatgaaac
    16981 gatagatttt tgaataggaa taagatcatg tttggatttt ttaagaaaga taaggctgtg
    17041 gaagtagagg ttccgacaca ggttcctgct catatcggca tcatcatgga tggcaatggc
    17101 cgttgggcta aaaaacgtat gcaaccgcga gtttttggac ataaggcggg catggaagca
    17161 ttgcaaaccg tgaccaaggc agccaacaaa ctgggcgtca aggttattac ggtctatgct
    17221 ttttctacgg aaaactggac ccgtccagat caggaagtca agtttatcat gaacttgcca
    17281 gtagagtttt atgataatta tgtcccggaa ctacatgcga ataatgttaa gattcaaatg
    17341 attggggaga cagaccgcct gcctaagcaa acctttgaag ctttaaccaa ggctgaggaa
    17401 ttgactaaga acaacacagg attgattctt aattttgctc ttaactatgg tggacgtgct
    17461 gagattacac aggcgcttaa gttgatttcc caggatgttt tagatgccaa aatcaaccca
    17521 ggtgacatca cagaggaatt gattggtaac tatctcttta ctcagcattt gcctaaggac
    17581 ttacgagacc cagacttgat tatccgtact agtggagaat tacgtttgag caattttctt
    17641 ccatggcagg gagcctatag tgagctttat tttacggaca ccttatggcc tgattttgac
    17701 gaagcggcct tgcaggaagc tattcttgcc tataatcgtc gtcatcgccg atttggagga
    17761 gtttaggagg aaatatgacc caggatttac agaaaagaac cttgtttgca gggattgccc
    17821 tggctatttt cctaccaatt ttaatgattg ggggcctctt gcttcagata gcaatcggaa
    17881 tcatagccat gctagccatg catgaacttt tgaagatgag aggtctagag accatgacga
    17941 tggagggcct cttgaccctc tttgcaacct ttgcattgac cattcccttg gagaattacc
    18001 tgactttttt gccagttgat gggaatgtgg ttgcctatag tgttttgatt tcaatcatgt
    18061 taggaacgac cgtttttagc aagtcttata cgattgagga tgcggttttc cctcttgcta
    18121 tgagcttcta cgtgggcttt ggatttaatg ctttactaga tgctcgtgtt gcaggtttgg
    18181 acaaggctct cttagccttg tgtatcgtct gggcgacaga cagtggtgcc tatcttgttg
    18241 ggatgaacta tgggaaacga aagttagcac caagggtatc gcctaataaa acccttgagg
    18301 gtgccttggg tggtatttta ggagcaattt tagtaaccat tatctttatg atagttgaca
    18361 gtacagttgc tcttccatat ggaatttaca agatgtcagt ctttgctatt ttctttagca
    18421 ttgctggaca atttggtgat ttactagaaa gttcgatcaa acgtcatttt ggtgttaagg
    18481 attctgggaa atttatccct ggacatggtg gtgttttgga tcgtttcgat agtatgttgc
    18541 ttgtatttcc aatcatgcac ttatttggac tcttttaatc aaaagacgga ggaaacgcta
    18601 tgctcggaat tttaaccttt attctggttt tcgggattat tgtagtggtg cacgagttcg
    18661 ggcacttcta ctttgccaag aaatcaggga ttttagtacg tgaatttgcc atcggtatgg
    18721 gacctaaaat ttttgctcac attggcaagg atggaacggc ctataccatt cgaatcttgc
    18781 ctctgggtgg ctatgtccgc atggccggtt ggggtgatga tacaactgaa atcaagacag
    18841 gaacgcctgt tagtttgaca cttgctgatg atggtaaggt taaacgcatc aatctctcag
    18901 gtaaaaaatt ggatcaaaca gccctcccta tgcaggtgac ccagtttgat tttgaagaca
    18961 agctctttat caaaggattg gttctggaag aagaaaaaac atttgcagtg gatcacgatg
    19021 caacggttgt ggaagcagat ggtactgagg ttcggattgc acctttagat gttcaatatc
    19081 aaaatgcgac tatctggggc aaactgatta ccaattttgc aggtcctatg aacaatttta
    19141 tcttaggtgt cgttgttttt tgggttttaa tctttatgca gggtggtgtc agagatgttg
    19201 ataccaatca gttccatatc atgccccaag gtgccttggc caaggtagga gtaccagaaa
    19261 cggcacaaat taccaagatt ggctcacatg aggttagcaa ctgggaaagc ttgatccaag
    19321 ctgtggaaac agaaaccaaa gataagacgg caccgacttt ggatgtgact atttctgaaa
    19381 aggggagtga caaacaagtc actgttacac ccgaagatag tcaaggtcgt taccttctag
    19441 gtgttcaacc gggggttaag tcagattttc tatccatgtt tgtaggtggt tttacaactg
    19501 ctgctgactc agctctccga attctctcag ctctgaaaaa tctgattttc caaccggatt
    19561 tgaacaagtt gggtggacct gttgctatct ttaaggcaag tagtgatgct gctaaaaatg
    19621 gaattgaaaa tatcttgtac ttcttggcaa tgatttccat caatattggg atttttaatc
    19681 ttattccgat tccagccttg gatggtggta agattgtgct caatatccta gaagccatcc
    19741 gccgcaaacc attgaaacaa gaaattgaaa cctatgtcac cttggccgga gtggtcatca
    19801 tggttgtctt gatgattgct gtgacttgga atgacattat gcgactcttt tttagataat
    19861 cgaggaatat tatgaaacaa agtaaaatgc ctatcccaac gcttcgcgaa atgccaagcg
    19921 atgctcaagt tatcagccat gctcttatgt tgcgtgctgg ttatgttcgc caagtttcag
    19981 caggtgttta ttcttatcta ccacttgcca atcgtgtgat tgaaaaagct aaaaacatca
    20041 tgcgccaaga attcgaaaag attggtgctg ttgagatgtt ggctccagcc cttcttagtg
    20101 cagaattgtg gcgtgaatca ggtcgttacg aaacctatgg tgaagacctt tacaaactaa
    20161 aaaaccgtga aaaatcagac tttatcttag gtccaactca cgaagaaacc tttacagcta
    20221 ttgtccgtga ttctgttaaa tcttacaagc aattgccact caacctttat caaattcagc
    20281 ccaagtatcg tgatgaaaaa cgcccacgta atggacttct tcgtacacgt gagtttatca
    20341 tgaaggatgc ttatagtttc cacgctaact atgatagttt ggatagtgtt tatgatgagt
    20401 acaaagcagc ctatgagcgt attttcactc gtagtggttt agacttcaag gctattattg
    20461 gtgacggtgg agccatgggt ggtaaggata gccaagaatt tatggccatt acatctgctc
    20521 gtacagacct tgaccgctgg gttgtcttgg acaagtcagt tgcctcattt gacgaaattc
    20581 ctgcagaagt gcaagaagaa atcaaggcag aattgctcaa atggatagtc tctggtgaag
    20641 ataccattgc ttactcaagt gagtctagct atgcagctaa cttagaaatg gcaacaaacg
    20701 agtacaaacc aagcaaccgt gttgtcgctg aagaagaagt tactcgtgtt gcaacgccag
    20761 atgttaaatc aattgatgaa gttgcagctt tcctcaatgt tccagaagaa caaacgatta
    20821 aaaccctctt ctacattgca gatggtgagc ttgttgcagc ccttctagtt ggaaatgacc
    20881 aactcaacga agtcaagttg aaaaaccact taggagcaga tttctttgac gttgctagcg
    20941 aagaagaagt ggcgaatgtt gttcaagcag gatttggttc acttggacca gttggtttgc
    21001 cagagaatat taaaattatt gcagatcgta aggtgcaaga tgttcgcaat gcagttgtcg
    21061 gtgctaacga agatggctac cacttgactg gtgtgaaccc aggccgtgat tttactgcag
    21121 aatatgtgga tatccgtgaa gttcgtgagg gtgaaatttc cccagatgga caaggtgtcc
    21181 ttaactttgc gcgtggtatt gagatcggtc atattttcaa actcggaact cgctactcag
    21241 caagcatggg agcagatgtc ttggatgaaa atggtcgtgc tgtgccaatc atcatgggat
    21301 gttacggtat cggtgtcagc cgtcttcttt cagcagtgat ggagcaacac gctcgcctct
    21361 ttgttaacaa aacgccaaaa ggtgaatacc gttacgcttg gggaatcaat ttccctaaag
    21421 aattggcacc atttgatgtg catttgatta ctgtcaatgt caaggatgaa gaagcgcaag
    21481 ccttgacaga aaaacttgaa gcaagcttga tgggagctgg ttatgaagtc ttgacagatg
    21541 accgtaacga acgtgtcggt gttaaattta gcgatagtga cttgattggt cttccaatcc
    21601 gtatcactgt tggtaaaaaa gcggccgatg gcattgtaga agttaagatc aaggcaactg
    21661 gtgacaccat tgaggttcat gcagataacg tgcttgaaac gcttgaaatc ctaagcaaga
    21721 aataaaaact ataatcagaa gaaaaacaag gaaaaaatgt aactagtttt taccttgttt
    21781 tttctgtata atgggagaaa tgagtagata aagaggtaaa tgttatgcta agatttccaa
    21841 aggattttgt ctggggatcc tctacttctg gaccgcagac agaaggacgt gtagctggtg
    21901 acggtaaggg agacaatctc tgggattact ggttccaagt ggagccaaat cgttactata
    21961 atgggattgg ttcagataag acatcgactt tttatgaaaa ttgggagcgg gatattgagc
    22021 ttttgttaga gactggtcac acagtctttc ggacttctat tcagtggtca cggatttttc
    22081 cacaaggctg tggaaaagtc aaccctcaag gtgtggattt ttatcgtaag gtctttgagg
    22141 ctattaaggc taaaggaatt cgtctgttag tcaatctcta tcattttgat ttaccttttg
    22201 cccttcaaga ggatggtgat ggttgggaaa ataaggcgac agtctcagcc tatgaagact
    22261 atgctcgttt ttgttttgag acttatggag atttagtgga tcaatggatt acctttaacg
    22321 agctcatcgt tcctgtagaa tttggttatt tttatgatgc ccattatcca cataaggtgg
    22381 atgcagaggc agctgttaag gtagcctatc atacacaatt ggccagcagc cgggctgtta
    22441 aggcttgcca tgaacttttg cctgattcca agattgggat tgtcctcaac ttgacaccgg
    22501 cttatccacg tagccagcat cctgctgatg tcaaggcagc tcgtattgcg gccctttttc
    22561 aggcccaatc tttcttagat ccatctgtct tggggactta tccacaggag ttggtagaaa
    22621 tcttgcatga acacggtctt ttacctgatg ctacagagga agagttggaa ctcattcatg
    22681 ataatacggt ggacttcctt ggtgtgaact actatcaacc tttgcgtgtt atggctcctc
    22741 gatttgctaa gcatccagag agtccactct taccagaaca tttttacgag ccttatgtga
    22801 tgcctggacg taaaatcaat tctcaccgtg gttgggagat ttatgagcaa gggatttatg
    22861 acatcgccca aaatatcaag gaaaattatg gcaatattga atggatgttg actgagaatg
    22921 gtatgggtgt tgaaggggaa gaaaaattcc gtcaagatgg aatgattcaa gatgattacc
    22981 gtattgactt tgtaaaaggt catcttcgtg aacttcaccg tgccattgaa gatggtgcca
    23041 actgtaaggg ctacttgatt tggaccttta ttgactgctg gtcatggctc aacagctata
    23101 aaaatcgcta tggtttggtc gaattagact tggaaacgca agaacgtcgt ctgaagaaat
    23161 caggccactg gttcaaaaaa ttaagcgata ataatggatt ttaaaaagga ctctgactga
    23221 ttatcccaat cggtcagagt tttttgctta caggagctta atttgaacta taccaatttt
    23281 tactcttgac aagtgaaaga aacaagtata tactgttttt gctgattcta taaaagatga
    23341 attagactat accaatttta aggagaaagc acagcttgcc tgtgtcgtat atactatgtg
    23401 tggaattgtt ggtgttgttg gaaacacaaa tgcaactgat attttgattc aagggcttga
    23461 aaagcttgaa taccgtggct atgattctgc gggaattttt gtcctagatg gtgctgataa
    23521 ccatttggtg aaggctgttg gtcgtatcgc agaattgtct gccaagacag ctggtgttga
    23581 gggaacaact ggtatcggac atactcgttg ggcaactcat gggaaaccaa cggaagacaa
    23641 tgctcaccca caccgctctg agacagaacg ttttgtcttg gttcacaatg gggtgattga
    23701 aaactacctt gaaattaaag aagaatacct tgctgggcac cacttcaaag ggcaaacaga
    23761 tacggaaatc gccgtacatt tgattggaaa atttgcggaa gaagacggtc tctcagttct
    23821 tgaagccttt aaaaaagctc ttcatattat ccgtggttca tatgcctttg ccttgattga
    23881 ctctgaaaat ccagatgtca tctatgtagc gaaaaacaaa tctccacttt tgattggtct
    23941 tggggaaggc tacaatatgg tctgctcaga tgctatggct atgattcgtg aaaccaacca
    24001 atacatggaa attcatgacc aagagttggt aatcgtcaag gctgatagcg tggaagttca
    24061 agactatgat ggaaatcgtc gtgagcgtgc tagctacact gctgaacttg acttgtcaga
    24121 tatcggtaag ggaacttacc cttactacat gctcaaggaa attgacgagc aaccaactgt
    24181 tatgcggaaa ctcatccaag cctacacaga tgaggctggt caagttgtcg tagacccaga
    24241 tatcattaag gctgttcaag acgcagaccg catctacatc cttgcagctg gaacatctta
    24301 ccatgcagga tttgcttcta agaagatgtt ggaagaattg acagatacac cagttgaact
    24361 tggaatctca tctgagtggg gctacggtat gccacttctc agcaagaaac cactcttcat
    24421 ctttatcagc caatctggtg aaacagcgga tagtcgtcaa gttttggtca aggctaatga
    24481 aatgggaatt ccaagcttaa cagtgacaaa tgttccaggt tcaaccctct cacgtgaagc
    24541 caactatacc atgctccttc acgcaggtcc tgaaattgcc gtggcatcaa ctaaagccta
    24601 tacagcgcaa atcgcagccc ttgccttcct tgcaaaagca gtcggagaag caaatggtaa
    24661 tgctaaagcg caagcctttg acctggttca tgaattgtca atcgtagctc agtctattga
    24721 atcaactctt tcagagaaag aaaccattga agccaaggtt cgtgaacttc ttgaaacaac
    24781 tcgtaacgcc ttttacatcg gacgtggtca agattattac gtagccatgg aagcaagtct
    24841 caaactcaaa gagatttctt atatccagtg tgaaggtttt gcggcaggag aactcaagca
    24901 cggaaccatt gccttgattg aagaaggaac gcctgttttg gctctcttgt cagatccagt
    24961 tcttgccaac catactcgtg gaaatatcca agaggtcgca gcccgtggtg ccaaagtcct
    25021 cactatcgca gaagagaatg tagccaaaga taccgacgat atcgtcctta cgaccgtaca
    25081 tccatacctc tcaccaattt caatggtcgt accaacgcaa ttagtcgctt actttgcaac
    25141 cctccaccgt ggcctcgatg tggacaaacc acgtaacctt gccaagtcag taacggtaga
    25201 ataagctaaa aaagtctagt tatctaggct ttttcttgtg agcaaattgg ttgcttgtac
    25261 tcaagattcg tgttatactc ttagaaaatc tcttcaaacc acgtcaccgt cgccttgccg
    25321 taggtatggt tactga
//
LOCUS       DAVBBS010000028        24327 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVBBS010000028 DAVBBS010000000
VERSION     DAVBBS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24327
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.028"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..618)
                     /locus_tag="VQU32_001113"
     CDS             complement(<1..618)
                     /locus_tag="VQU32_001113"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET2472576.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEK"
     gene            complement(769..2079)
                     /gene="secY"
                     /locus_tag="VQU32_001114"
     CDS             complement(769..2079)
                     /gene="secY"
                     /locus_tag="VQU32_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET2472577.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            complement(2092..2532)
                     /gene="rplO"
                     /locus_tag="VQU32_001115"
     CDS             complement(2092..2532)
                     /gene="rplO"
                     /locus_tag="VQU32_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET2472578.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            complement(2677..2901)
                     /gene="rpmD"
                     /locus_tag="VQU32_001116"
     CDS             complement(2677..2901)
                     /gene="rpmD"
                     /locus_tag="VQU32_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET2472579.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            complement(2873..3367)
                     /gene="rpsE"
                     /locus_tag="VQU32_001117"
     CDS             complement(2873..3367)
                     /gene="rpsE"
                     /locus_tag="VQU32_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET2472580.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            complement(3385..3741)
                     /gene="rplR"
                     /locus_tag="VQU32_001118"
     CDS             complement(3385..3741)
                     /gene="rplR"
                     /locus_tag="VQU32_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET2472581.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(3825..4361)
                     /gene="rplF"
                     /locus_tag="VQU32_001119"
     CDS             complement(3825..4361)
                     /gene="rplF"
                     /locus_tag="VQU32_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET2472582.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(4553..4951)
                     /gene="rpsH"
                     /locus_tag="VQU32_001120"
     CDS             complement(4553..4951)
                     /gene="rpsH"
                     /locus_tag="VQU32_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET2472583.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(5236..5505)
                     /gene="rpsN"
                     /locus_tag="VQU32_001121"
     CDS             complement(5236..5505)
                     /gene="rpsN"
                     /locus_tag="VQU32_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HET2472584.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            complement(5523..6065)
                     /gene="rplE"
                     /locus_tag="VQU32_001122"
     CDS             complement(5523..6065)
                     /gene="rplE"
                     /locus_tag="VQU32_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET2472585.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            complement(6089..6394)
                     /gene="rplX"
                     /locus_tag="VQU32_001123"
     CDS             complement(6089..6394)
                     /gene="rplX"
                     /locus_tag="VQU32_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET2472586.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            complement(6472..6840)
                     /gene="rplN"
                     /locus_tag="VQU32_001124"
     CDS             complement(6472..6840)
                     /gene="rplN"
                     /locus_tag="VQU32_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET2472587.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            complement(6866..7126)
                     /gene="rpsQ"
                     /locus_tag="VQU32_001125"
     CDS             complement(6866..7126)
                     /gene="rpsQ"
                     /locus_tag="VQU32_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET2472588.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            complement(7151..7357)
                     /locus_tag="VQU32_001126"
     CDS             complement(7151..7357)
                     /locus_tag="VQU32_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET2472589.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            complement(7367..7780)
                     /gene="rplP"
                     /locus_tag="VQU32_001127"
     CDS             complement(7367..7780)
                     /gene="rplP"
                     /locus_tag="VQU32_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET2472590.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(7784..8437)
                     /gene="rpsC"
                     /locus_tag="VQU32_001128"
     CDS             complement(7784..8437)
                     /gene="rpsC"
                     /locus_tag="VQU32_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET2472591.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(8450..8794)
                     /gene="rplV"
                     /locus_tag="VQU32_001129"
     CDS             complement(8450..8794)
                     /gene="rplV"
                     /locus_tag="VQU32_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET2472592.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            complement(8806..9087)
                     /gene="rpsS"
                     /locus_tag="VQU32_001130"
     CDS             complement(8806..9087)
                     /gene="rpsS"
                     /locus_tag="VQU32_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET2472593.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            complement(9191..10024)
                     /gene="rplB"
                     /locus_tag="VQU32_001131"
     CDS             complement(9191..10024)
                     /gene="rplB"
                     /locus_tag="VQU32_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET2472594.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(10042..10338)
                     /locus_tag="VQU32_001132"
     CDS             complement(10042..10338)
                     /locus_tag="VQU32_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET2472595.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(10338..10961)
                     /gene="rplD"
                     /locus_tag="VQU32_001133"
     CDS             complement(10338..10961)
                     /gene="rplD"
                     /locus_tag="VQU32_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET2472596.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            complement(10986..11612)
                     /gene="rplC"
                     /locus_tag="VQU32_001134"
     CDS             complement(10986..11612)
                     /gene="rplC"
                     /locus_tag="VQU32_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET2472597.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            complement(11828..12136)
                     /gene="rpsJ"
                     /locus_tag="VQU32_001135"
     CDS             complement(11828..12136)
                     /gene="rpsJ"
                     /locus_tag="VQU32_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET2472598.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(12402..13019)
                     /locus_tag="VQU32_001136"
     CDS             complement(12402..13019)
                     /locus_tag="VQU32_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HET2472599.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            complement(13016..13606)
                     /gene="nrdG"
                     /locus_tag="VQU32_001137"
     CDS             complement(13016..13606)
                     /gene="nrdG"
                     /locus_tag="VQU32_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HET2472600.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVAGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKEIYEQVKRE"
     gene            complement(13599..14105)
                     /locus_tag="VQU32_001138"
     CDS             complement(13599..14105)
                     /locus_tag="VQU32_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2472601.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDVEDFSYEEWL
                     EGNRNKEMGIWLPENRVPSIQFVLFGESGCALGFLNLRLRLNEGLLNYAGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            complement(14149..14289)
                     /locus_tag="VQU32_001139"
     CDS             complement(14149..14289)
                     /locus_tag="VQU32_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472602.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            complement(14301..16508)
                     /gene="nrdD"
                     /locus_tag="VQU32_001140"
     CDS             complement(14301..16508)
                     /gene="nrdD"
                     /locus_tag="VQU32_001140"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HET2472603.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNCRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            complement(16628..18166)
                     /locus_tag="VQU32_001141"
     CDS             complement(16628..18166)
                     /locus_tag="VQU32_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET2472604.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            complement(18203..18346)
                     /locus_tag="VQU32_001142"
     CDS             complement(18203..18346)
                     /locus_tag="VQU32_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472605.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            complement(18424..19956)
                     /gene="cls"
                     /locus_tag="VQU32_001143"
     CDS             complement(18424..19956)
                     /gene="cls"
                     /locus_tag="VQU32_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HET2472606.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            complement(20102..20659)
                     /locus_tag="VQU32_001144"
     CDS             complement(20102..20659)
                     /locus_tag="VQU32_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HET2472607.1"
                     /translation="MTPLFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQQVQQPAQQPAQQNTNTANAGGNQNQAAPVQNQPVAQP
                     TDIDGTYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKI
                     YTVNGNQIVVDDMDRDPSDQIVLTK"
     gene            complement(20656..21906)
                     /locus_tag="VQU32_001145"
     CDS             complement(20656..21906)
                     /locus_tag="VQU32_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET2472608.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRIQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFEMLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNFSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGALKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSCREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            complement(22114..22419)
                     /locus_tag="VQU32_001146"
     CDS             complement(22114..22419)
                     /locus_tag="VQU32_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HET2472609.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            complement(22435..22854)
                     /gene="ruvX"
                     /locus_tag="VQU32_001147"
     CDS             complement(22435..22854)
                     /gene="ruvX"
                     /locus_tag="VQU32_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET2472610.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPLDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            complement(22858..23124)
                     /locus_tag="VQU32_001148"
     CDS             complement(22858..23124)
                     /locus_tag="VQU32_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HET2472611.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            complement(23211..23780)
                     /locus_tag="VQU32_001149"
     CDS             complement(23211..23780)
                     /locus_tag="VQU32_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2472612.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            complement(23846..24244)
                     /gene="spx"
                     /locus_tag="VQU32_001150"
     CDS             complement(23846..24244)
                     /gene="spx"
                     /locus_tag="VQU32_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HET2472613.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
BASE COUNT         6740 a         5398 c         4560 g         7629 t
ORIGIN      
        1 tttttcaata tctgagaaga catcattgat atcttgatta ccttcgatgt catgaaccaa
       61 acctttggca cggtagtgag caatgattgg ttctccttga gcaatattaa catccaaacg
      121 acgttttact gtctcaggct tatcatcttc acgttggtag taatcttctt ctttatagtc
      181 aactggtggg ttaaagacct tgtggaaagt ttctccagtt acgcggtgga tgatacggcc
      241 actcaaacgt tccaagaggc tgtcagggtt cacttcaata ttgataatac cttctagttc
      301 aatgccaagt tcagccaatg ttttgtccaa ggcatgagct tgttcaattg tacgtgggta
      361 accatccaat aagaatcctg tttctttaat atcatcttgt gaaaggcgtt cttttacgat
      421 tccatttgta acttcgtcag gaaccaattc acccttgtca atatatgact tagcaagaac
      481 acccatttca gtttgatttg ccattgcagc gcggaacata tcacctgttg agatatgtgc
      541 aacatggaat tgttctacga tttttgctgc ttgagttccc ttacctgcac caggtaagcc
      601 cataatcaaa agattcatga tttgatctcc ttattttatt tttaaataga agcaaaaagt
      661 cttttcaccc ctatttaaaa acaaaataga agaggggaga tccaactctt acaaatgtca
      721 gagtttaaac ctccactccc tcagtattta ctgattcagt aaatactttt attctgttct
      781 gtccatgaaa ccaacatact tacgtttcaa taggtaacct tccaattgct tgattccttc
      841 gatacctgta gagataatga tcaagagact tgttccacca aaggcaacaa catcagaaag
      901 accaaataca tctttagctg caatcggtaa aatggaaatc acaccaagga agagggaacc
      961 aacagttgca agacgacgaa gaagtttaga catatattct tctgtacctt taccaggacg
     1021 aactccatgg atataggcac cactcttttg taggctctct gctgcttttt caggattaat
     1081 ctgtacaaac gtatagaaga atgtaaagag aataatcaac aaagcataca tggcaatacc
     1141 agttggagaa gtagttgcca acatctcttg tgctaccctt acccaagccc aatcatgacc
     1201 tgtggcactc aaaaactgaa gaatagccgc aggcgctgca gtaatcgaac tggcaaagat
     1261 aacagggata actccagcag ggtttacctt taacggaagg taagagctag atggagcacc
     1321 ttgtgcaacc ttagtatatt ggattggaat tttgtattct gcttgttgaa cataagttgt
     1381 aaagtaaata atcaacaata cagtaataat caaaatgatt acgaaaatga tagatgaagt
     1441 gatacggcta cttgggacgt tcacaaagta gtccacatag atgccctgaa tcatctctgg
     1501 aattgaggaa acaatcccgg caaagataat catggaaaca ccgtttccgt atcccttatc
     1561 tgtaatttgc tcacccaacc aagtgacaat catactacca gctgttaaga tgataccaat
     1621 cgtcagaaaa acttgtggag ttaaagcagt tttaatcaat tgagctccag ccaaggtatt
     1681 aaaaccagct gtaatcccga tagattgcac aaaagcgaga actagagcaa tataacgagt
     1741 agcttgattc aattttcttc gacctacttc cccttgttta ccccactcta caaacttggg
     1801 taaaatatcc atttgcaaga gttggacaac aatagaagcg gtgatatagg gactaactcc
     1861 tagggcaaaa atcgaaaagt tttttagggc attccccgac accaagctca acatgtttaa
     1921 gaaggataat ccacttaaag cattcaagct attggcattc acaccaggaa ctgtaatgct
     1981 agttccgata cgaaagacca aaacgataaa aattgtaaat aaaatttttg atcgaacctg
     2041 cttgacttta agagcttctc ttaataattt aaaaaacata ggtcacctct cttagatgac
     2101 ttctactgaa ccacctttag cagtgatagc ttcttcagct gatttagaga atttagctgc
     2161 tttcacagtc aatttcttag tcaactcacc gttaccaaga attttaatac ctgacttttc
     2221 agctttaaca attcctgctt cgataagaac aactggagtt acttcagcac catcttcaaa
     2281 gacgttcaat tggtcaaggt tcacaattgc gtattcttta gcgttgatgt tagtgaatcc
     2341 acgttttgga agacgacgga acaatggagt ttgtccacct tcaaaaccaa ggcgaactcc
     2401 gccaccgcta cgagcttttt gacctttttg accacgacca gatgttttac cgttacctga
     2461 tgaagtacca cgaccaacgc ggttacgtac tttacgagaa ccttctgcag gtttcaattc
     2521 atgaagtttc atttttattt tctcctcttt tgtaaaatgc tagcgccgat aagggagaaa
     2581 aggttgtctc ccccatcaac tcgcctatac gatgtcatca tagatgacta tatctagttt
     2641 taggggatgg tacagtgcac atcccctaaa acttcattag tttacttctt caactgttac
     2701 taagtgagat actgctgtaa tcataccacg gatagcagcg ttatcttctt taataacaga
     2761 gctgttcaat ttgccaagtc caagtgctac aacagtttta cgttgtgatg gaatgcgtcc
     2821 gattggagac ttagtcaaag taattttaat ttgagccatt ttatcccctt tcttatgcca
     2881 aatcagaaac tgaaatacca cgaagggcag caatttcttc agcgcgtttc aattgtttca
     2941 aaccttcaac agttgcacga acaatgttga ttggagtgtt agaaccaagt gatttagatg
     3001 taatatctgc cacacctgcc aattccacaa cggcacgaac tgcaccacca gcggcaactc
     3061 cagaaccttc tacagcaggt ttcaacaata ctttagctcc accgaattct gaaagaactt
     3121 cgtgtgggat tgttgttcca accataggaa cttcgatcaa gtttttctta gcatcatcta
     3181 ctgctttacg gattgcttct ggaacttctt gagctttacc agtaccaaat cctacgcgac
     3241 cattgtggtc accaacaaca acaagagctg cgaaacgaag acgacgtcca cctttaacaa
     3301 cttttgtaac acggttgaca gcaactacgc gttcttctaa ttcaactgca ttgtctttaa
     3361 atgccatttt ctagtgtcct cctattagaa tttcaatccg ttttcacgag ctgcatcagc
     3421 caaagctttc acacgtccgt gatatagata tccaccgcgg tcgaacacca cttctgaaat
     3481 acctttagcg tttgcacgtt ctgcaacgag tttaccgaca gcaacggctt gttcagtttt
     3541 agttcctttt gaaacttctt tatcaagagt tgaagcactt gcgagcgtta cacccgctac
     3601 gtcatcaatc acttgagcgt agatgcctgt attagaacgg aatacgttca aacgtgggcg
     3661 atcagcagtt ccagagagtt ttccgcgaac gcgacggtgg cgtttttggc ggagtttgtt
     3721 tttatctggt tttgaaatca cagttttcac ctctttagtt ttaaatcgtg tgctatgcac
     3781 aaagttggaa aataggttgg tggttgatga tcaaccactc aacattattt acctgtttta
     3841 ccttctttac ggcgaacgaa ttcaccaacg taacggatac ctttaccttt atatggttct
     3901 ggtgaacgaa ggctacgtac gtaagcagct gtttgaccaa ctacttcttt tgaaattccg
     3961 ctaacaacga ttgttgttgg gtttggaagt tcaaaagtaa ttccttctgg agcttcaact
     4021 tcgtctggat gagatttacc aacagccaaa acaagtttag atccttgaag ctgtgcacgg
     4081 taaccaaccc cacgcatttc aagttctttc ttgaatcctt ctgatacacc aacaaccatg
     4141 ttgttcaaaa gggcacgagt agttccgtgg atagttttca tttcttttga atcgtttgga
     4201 cggtgaagag ttatttcagt accttccaca cggatttcaa tatcttttga gaactcacga
     4261 gtaagttctc ctttaggtcc ttttacagtt acaacgttgt cattgttagc gagttcaaca
     4321 ccagcaggca acacgataac tttattacca atacgtgaca tgtttatttt ctcctgttaa
     4381 attgtcaggc cagaacggcc agttttcacg gggttcagat acttatttag ttcaagagct
     4441 gaactgaaca cgctctaaga actgtgcaaa atagataagt tggcttgttt gtaaacaaac
     4501 ttctccaact tcctaatttt gctttgttct ttacgagctt tgtatcttga ttttaccaaa
     4561 cgtaagcgat aacctcacca ccaacattct tttggcgtgc ttctttatca gtaagcaaac
     4621 cttcagaagt tgaaaggatg gcaattccaa gtccgttaag aacttttgga aggtcttcac
     4681 gttttttgta gacacgaagt cctggtttag aaacacgttt caagttagtg ataactttct
     4741 caccatttgg tccgtattta agaaatacac ggatgacgcc ttgtttgtca tcttcaatga
     4801 tttcaacgtt ttttacaaaa ccttcgcgtt taaggatttc agcaatccct tttttgatgt
     4861 ttgatgcagg tacttcaagt acttcgtgtt tagcttggtt agcattacga atacgagtta
     4921 ggaagtctgc gattgggtca gtcataacca ttttgttttt ctcctcttac tagtagtttg
     4981 caagttgcac ttgctagtta atacatgata caaagagcgt aacagcacga gcaaaaatag
     5041 acaatttaat gcagaagcaa tgctccaaag caaattggtc ttttttgcca aagctgtagc
     5101 tcgtgttcaa attggcaagc ccaattgaac ccgggctaaa ctctgtgtga aaaagataaa
     5161 ctttcctaga aactttagtt tcttcgtcaa gtttcctatt tttacttgga gttttgacgc
     5221 tcttgatatc ttaaattacc aagatgcttt tgttacacca ggaatttgac ctttatgcgc
     5281 aagttcgcga aaagcgatac gactcagacc aaatttgcgg taaactgaat gtgggcgccc
     5341 cgtaactcta caacgattat gtaaacgagt cggtgaggcg ttgcgaggta atttagataa
     5401 accttcgtag tcccctgccg cctttaatgc agcacgtttt tcagcataac ggtcaacaat
     5461 tttttggcgt ttagcctctc tagctaccat tgatttttta gccattagat ttacctccta
     5521 tattattttg caaaaggcat tccaaggcct gtaagcaatg cacgtgactc ttcgtcagtg
     5581 ttagcagttg ttacgataac gatgtcaaga ccacgagttt tgtcaacgtc atcgaagttg
     5641 atttctggga agattaattg ttctttcaca ccaagtgtgt agttcccgcg tccatcaaat
     5701 gattttgttg gaacaccgtg gaagtcacgt acacgtggaa gtgaaactga taccaattta
     5761 tccaagaatt cgtacatacg ttcaccacga agggtaactt ttgcaccgat cgcaacacct
     5821 tcacgaagac ggaagccggc gattgatttt ttagctttag tgataagtgg tttttgacct
     5881 gagataagtg ccaattcttc agcagctttt tcaaggcttt tagcgtttga tacagcttca
     5941 ccaacaccca tgttcaaaac aatcttatct actttaggca cagccatcac tgatgagtag
     6001 ttgaattgtt ctgtcaaagc aggaactact tcattaagat atttttcttt taaacgattt
     6061 gccattatac ttctcctttc cttcgtgatt aatcaagcac ttcgcctgat tttttgttgt
     6121 agcgaacttt tttaccgtct acaaatttgt aaccaacacg accagctaca ccatttttgt
     6181 ccaaaacttg aacgtttgat acgtggatag ctgcttcttt ctcgatgata ccaccttgag
     6241 gaagctcgtt agttggacgt tggtgtttct taacaatgtt aacaccttca acgataactt
     6301 tgtttacttt tggaagggca gtaaggacaa cagcttctgt tcccttatct ttaccagcga
     6361 ttacgcgaac tttgtcgcct ttttttacaa acattaggtt tctccttgat ttttcttacg
     6421 cccataaggg caccctagct tgaagctagg ggactagttt gttcctaaaa attaaagtac
     6481 ttctggagca agtgacacga tcttcatgaa gccaccttca cgcaattcac gtgcaactgg
     6541 gccaaagata cgtgttccgc gaggagtttt gtcttcacgg atgataactg ctgcgttttc
     6601 gtcaaatttg atgtatgaac catcagcacg acgagcacct gatttagtac gaacgataac
     6661 tgctttaaca acgtcacctt ttttaaccgc accaccagga gtagcttgtt ttacagatgc
     6721 cacgataaca tcaccgatgt ttgcaaattt acgtcctgaa ccaccaagaa ctttgatagt
     6781 caagatttcg cgagcaccgc tgttgtctgc gactttcaaa cgagtttctg tttgaatcat
     6841 ttcagttttc tcctttcagg tttgattaga tgatgaccgc ttcttcaaca acttctacaa
     6901 gacggaaacg ttttgtagct gaaagcgggc gagtttccat gatacgtacg atatcgcctt
     6961 ctttggcaac attgttttca tcatgagctt tgtatttttt agagtagtta atacgtttac
     7021 catagactgg gtggttacgt tttgtttcaa ctacaactgt gattgtcttg tccattttgt
     7081 cagatacaac acgtccaaca agaactttac gattattgcg ttccattgaa atttctcctt
     7141 ccctagtcta ttatttcgct tcagattgaa ctgttttgat acgagcgatt tgttttttaa
     7201 cttctttcaa gcgagctgtt tgttccaatt gaccagtagc agcttggaaa cgaagttcaa
     7261 acaattcttt tttcaattcg ttttcgcgct tcgcgagttc ttcttgagaa agaccacgaa
     7321 gttctttaac aaattctttt acttcattaa gtttcatgcc ttctccttat tctgcttcac
     7381 gttttacgaa tttacattta actggcaatt tgtggctagc aagtcgaagc gcttcacgtg
     7441 caatctcttc agatacacca gcgatttcga acatcacttt accacgttta actggtgcta
     7501 cccaaccttc aggtgcccct ttaccagatc ccatacgcac accgatagct ttagcagtgt
     7561 atgatttgtg tgggaagatt ttaatccaaa ctttaccacc acgtttcatg taacgagtca
     7621 tggcgatacg agcagcttcg atttggcggt tagtgatcca gtggctagtt gtagcttgaa
     7681 gaccgtattc accgaatgct acttcttttc caccttttgc ttcaccgcgc atttttccac
     7741 ggaactcacg acggtgttta acacgtttag gtactaacat tggttattta cctcctttag
     7801 tgtttttacg agctggaaga acttcaccac ggtagatcca tactttaaca ccaagtttac
     7861 cgtatgtagt atctgcttct tcccaagcgt aatcgatatc tgcacgaagt gtgtgaagcg
     7921 gaacagttcc ttcagagtat ccttcagcac gggcgatatc tgcaccgttc aaacgacctg
     7981 atacttgagt tttgattcct ttagctccag cacgcattgc acgttggatt gcttgttttt
     8041 gtgcacgacg gaaagcaaca cgttgctcca attgacgagc aattccttca cctacaaggt
     8101 gagcatccaa atcaggttgt ttgatttcga tgatgttgat gtgtacttgt tttccagtca
     8161 atttgttaag ttttgcacgg agtgcatcaa cgttagcacc acctttaccg ataaccatac
     8221 ctggtttagc agtgtgaagt gaaacgttaa ctttgtttac tgcgcgttcg atttcaatag
     8281 ttgaaactgc tgcgtcagca agttcttttt gaacgaattt acggattgca agatcttcat
     8341 gaaggtaatc cgcgtattct ttttcagcat accatttggc atcccaatca cggatgatgc
     8401 cgacacgcat accaattgga tgtacttttt gacccacgat tttacctcct tatttttctg
     8461 caacagctac agtgatgtga gctgtacgtt tgttgattgg tgaagctgaa cctttcgcac
     8521 gtggacggaa acgtttcata gttggtcctt cgtttgcgaa tgcttcagat actaccaaat
     8581 tagctttatc caaaccaaag ttgttttcag cgttagctac agctgagttc aaaactttca
     8641 agatgatttc agcagctttg tttggagtga atgtcaagat tgcgattgca tcggctacgc
     8701 ttttaccacg gatgttatca agaacaagac gtgatttacg aggtgaaaca cgtactgtac
     8761 gagccattgc tttagctgaa gtaatttctg ccatttatgt tctccttatt ttctacgtgt
     8821 tttcttgtcg tctgcagcgt gacctttgta agtacgagtt ggtgcaaatt caccaagttt
     8881 gtggcctacc atgtcttctt ggatgtaaac aggtacgtgt ttacgtccgt cataaactgc
     8941 aatagtgtaa ccaatgaaac ttgggaagat cgttgaacga cgtgaccaag ttttaataac
     9001 ttttttcttt tcgtcgttag cttgagcttc aacttttttc atcaaatgct catcgacgaa
     9061 aggtcctttt ttaagactgc gtcccatttt tatattttct cctttaaatg ttgtaccaca
     9121 gcggcttgca ctcctatgga gcgctaccga gctggcggat ttactagttg cttaagcgac
     9181 tagtttaata ttatttctcg ttgcgacgac gaacgataag tttgtcagat ttcgctttct
     9241 tgttacgagt tttaagacca agagcaggtt tgccccatgg agtagatggt gctttacgac
     9301 caactggtgc tttaccttca ccaccaccgt gtgggtgatc gttagggttc attacagaac
     9361 cacgaactgt tgggcggata cctttccaac ggctacgtcc tgctttacca aggtttacaa
     9421 gtccatgttg ttcgtttccg acaacaccaa ctgtagcacg gcaagttcca agaatcatac
     9481 gaacttcacc tgattgaaga cgaacaagaa catatttacc ttcagaaccc aatacttgag
     9541 cagatgcacc agcagcacgt accaattcac caccacgacc tggtttcaac tcgatgttgt
     9601 gaatcaaagt accaactggg atgttagcaa gtggaagagc gtttccgact ttgatatctg
     9661 cttctggacc tgaaacgata cgttgaccta cttcaagacc ttttggagcg atgatgtatg
     9721 ctttcacacc gtcagtgtag tgtacaagag cgatgtttgc agaacggttt ggatcgtact
     9781 cgattgtttt aacaactgct tcaacgttgt ctttattacg tttgaagtca accaaacggt
     9841 aaaaacgttt gtgtccacca ccttggtgac gaactgtgat acgaccgttg ttgttacgac
     9901 cagccttgct cttcaatgca acaagcaatg atttttcagg agtgcttgtt gtgatttcag
     9961 cgaaatccaa agaagtcata ttacggcgac cgtttgttgt tggtttataa acacgaattc
    10021 ccacgatatt tcctccttag attattcagc ttcagcagca aacaactcga ttgctttaga
    10081 atcagctgta agtgtgatga tagctttttt agttttgtta gtaaaaccag tgtaacgtcc
    10141 aacacgttta gcttttggtt ttacgttgat tgtgttaaca ttggcaactt taacaccttc
    10201 gaaagcagct tcaacagctt gcttgatcaa aagtttgtgt gcacgagtgt caacttcaaa
    10261 tacatatttt cctgcttcaa gttgagccat tgagctttca gtgatgacag gttttttgat
    10321 aacatcatac aaattcatta tgcaagaacc tcctcgattt tagagatagc tgcttgtgtg
    10381 acaagaagtt tgtcgctatt tgcgatgtca agaacacttg cagttgtagc agttgcaact
    10441 ttcacgtttg gaaggttacg agctgaaaga gctgcgaatt catttccttc ttcaaggata
    10501 acaagaactt tagaatcgat gctcaatgct gcaagaactt ttgcaaattc agcagttttt
    10561 ggagctgtaa atgaaagagc gtctacagct acgaatttgt tttcagcaac tttttcagag
    10621 taaactgatt taagagctag gcgacgaact ttttgtggaa gtttgtagcc gtatgaacgt
    10681 ggagttggtc cgaagacaac accaccacca cgccattgtg gtgagcggat agaaccttga
    10741 cgagcacgtc cagttccttt ttgacgccat ggtttgcgtc caccacctga tactgcagag
    10801 cggtttttaa cagcgtgtgt tccttgacga aggcttgcgc gttggctgat gattacatca
    10861 aacacaactg attcatttgg ttcgatacca aatactgcat cgctaagaac aacttggcca
    10921 gcttctttac cagtttggtc aaataatgtt acgtttgcca ttgtgactga tttccccttt
    10981 ctttattatt taccagcttt aactgctgat ttgatagtga taagagattt cttagcacct
    11041 ggtacgttac ctttgataag gataacgttc ttttctggaa caacttgtac aacttcaagg
    11101 ttttgaattg ttacgcggtc gccacccata cgccctgcaa ggtttttacc tttgaatacg
    11161 cggttaggtg caacaggtcc catagaacct ggacgacggt ggtaacgaga accgtgagcc
    11221 attggtccac gtgattgtcc gtggcgtttg ataacacctt ggaaaccttt acctttagaa
    11281 gtaccagtta cgtcaacaac gtctccagct gcgaatgttt caactgtaat ttcagcacca
    11341 acttccaagc cttcaacgtt tttgaattca cgaatgaagc gcttaggagc cgtgttagct
    11401 ttcgctacat gtcctttagc aggtttgttg ctcaatactt cgcgtttgtc atcgaaacca
    11461 acttggatag cgttgtatcc gtctgtttca acagttttaa cttgaagaac aacgtttgga
    11521 gttgcttcaa taactgttac agggatcaat tcgccagctt cagtgaagat ttgagtcatt
    11581 cccacttttt tccctaagat tccttttgtc atgagaaaat agttcctttt ctatattttt
    11641 attcaaaaag tttttaacga gcgtttttta tgctcaagtc taagatacaa agggcgtcaa
    11701 actcaacgtg aaaataggaa actgacacag tgtctagcag acgcgaggaa gtttatcttt
    11761 ttcacaccga gtttagcccg tgttcaatta gattgaaaca ccagcctctg ctcttttata
    11821 ttttagatta aagtttgatt tctacgttta caccacttgg aagatccaat ttcatcaagg
    11881 catcaactgt tttttgagtt gggttaacga tatcgatcaa acgtttgtgt gtacgcattt
    11941 caaattgttc gcgagagtct ttgtatttgt gagtcgcacg aatgattgtg tagaggctac
    12001 gttcagttgg aagtgggatt ggacccgcaa cttgtgcacc tgtacgagta gctgattcta
    12061 cgatttttgc agccgctgtg tcaagcgtac ggtgttcgta agctttcaaa cggatacgga
    12121 tttttttgtt tgccatcttt ttctcctttt cgtctattta agataatagg ctagctccac
    12181 aagaaaaccg acgcggtgtt gcgtggcaat gcaaccgagc gtgtcgcaac ctcttgcatc
    12241 aaaactaagg ctgtaattta cagcaccata atagaataac acaaatcccc tgcgattgca
    12301 agggatttgt taggtttttt cgttttttgg ggatgaaact gttttcctac tttttctttc
    12361 taaaattagg gattttcttc tatatttctt ctatacataa gttaagtcct cttgacatca
    12421 aatttatctt ctgattggtc cactaagata tccgccttag actcagtttc tttatagtat
    12481 cgcagatact gctcccgtct catctgatgg ctagccaata taaaggatgc atcgcgattt
    12541 ctcacagtcg tatctctagc aaggcgtcgc tttaattcgg tctcctcatc cgtgtagaaa
    12601 cagatggttt tttcaaagag ttccttgggt agaaagccaa cagacatccc ttcgacaatc
    12661 aaaattggtt tggctccaga caagacctca ctagccttcc aaggttcttc aattgtcaag
    12721 acatccatac ccgcctgcaa ggcaaggata tctctctgca aactctccag ttcatgcgcc
    12781 actggcagac tggctgtcac cttttgattc ggcgcgtcct tgggtactac caaatagcgt
    12841 ccggaggtga tatagggatc tgtttctagc aaatttactg tagtagaatc taaagcttgg
    12901 tacaattcct gtgcaaaggt tgatttacct gaagctccat gaccgtatat tcccagtgtc
    12961 ctgactttcc ccgtctcgat ctctgagact agttggtcta ctaagtcttt tttcttcatt
    13021 ctctcttcac ctgttcatag atttcttttc cgtcattgag cttgtcccaa atcactactt
    13081 gcccactttt gagcgatttt tgcacatcga taattcgttg gttagacgaa cctcgaaact
    13141 ggagcataag atttctctta gttcgatcat atcttccatc gacaagaatg tcaatcagtg
    13201 acaagagttc cagtttatct ggagtttcca acatcatttc ttcccaagtg tagcccgtcc
    13261 aggaccagat gtccttgtct ggcaattcct tccgaatccg cttaacaagt ggcaagagaa
    13321 tcccagtatt aagaaaaggc tcccctccta gtaaggttaa cccctgaaca taaggctggg
    13381 caagatctgc cataatctgt tcttccaact cagctgtata gggaatgcct gcattgaagg
    13441 accaggtcgc tacattataa cacccctcgc agtgaaacat acagcctgct acatagagag
    13501 agttacgcac gccttcgcca tcaacaaagt taaaggcctt gtagtcaatg atacgacctt
    13561 gactaagttc ctcgcttttc cattcttgtg gttttggatt attcattcgc tacctctatc
    13621 caataacgct cgactccatt gcgagcatcc tcaaatattc caccatttgc tagaatgact
    13681 gctctgctag caggattatt cacgctacag gtcaccagag ctttcttgat gttcttttcc
    13741 ttagcaactt gcaagccctg acggagagtc tcttttgcat aacccttgcc tctttcagat
    13801 ggacggatgg agtagccaat gtggccagca taattcagta acccctcatt cagtctcaat
    13861 cgcagattca aaaatcctag agcacaacct gattcaccaa acaatacgaa ctgtatcgat
    13921 ggaacgcgat tttcaggtag ccatattccc atttctttat tacggttacc ttctagccac
    13981 tcctcgtaag aaaaatcctc aacatcccaa aatcctccat catgggctga ttggctcttt
    14041 tcaaactctg tcatcatatc taaaacagct ttcttatccg ctaatcttgg tctgcgtaat
    14101 tccatcctta cctcccacta tgagaaaagc gagagctggc tctcactttt attttttctt
    14161 gttcttgttt aaaaattgtt ctctgaagct aagcaagcgt tctttcttac cagctccacc
    14221 tttagagtgt ttctcgtgat aacgggtcac ttgtgcgcgc cccttatcgt ctagttgata
    14281 ttttcccatt tcatttcttt ctaatttgtt acttgatgcc cagctatttt aatcgttgaa
    14341 ccattcatat gtttgacacg cgcagcgatt tccttgtgac gcccgttgac catcggtctt
    14401 gcttgaggat tacctaggta gccacaagtt cgcttcacca catctactgt tttagggtcg
    14461 ctattgccac agtttggaca agcaaaccct ctctcagttg gttcaaaatc cccttcaaag
    14521 tcacacttgt agcaacggtc aatcggagta ttggtgccta gatagcctac acggtcataa
    14581 gcataatccc agacagcttc caaggccttt ggattttgct gaaggactgg atactcacaa
    14641 taatggatga aaccacctga cgcacctgct tctggataga ctttctcaaa gtccaatttt
    14701 tcaaacggtg ttggattttt acgaacatcg tagtggaaag agttggtgta gtattccttg
    14761 tctgtgatat caggaataga gccaaacttg tctatatcta gtcggcagaa acggtctgtc
    14821 agactttcgg atggtgttga gtagatagag aaatggtagc catattggtc tgaccactct
    14881 tctacacggc gtttcatatc gtgaatgatg tctagcgtga attccttagc atctggatta
    14941 ctttcccagc tgttaccaaa gaaaactgtc gctacttcat acaagccgat atagcccagc
    15001 gaaacggtcg cacgacaatt cttaaagagc tggtcaacac tttcttcttt acctagacga
    15061 tggccaaaag caccgtactg ataaaggata ggagcattcg ctggtgtcgc ctctttagtg
    15121 cgttcgacac ggtaaacaag agcatcttct gcgatattca ttcgctcgtt gaagatttcc
    15181 cagaacttat tcatatcacc ttcagactca agagcaatac gaggcagatt aaccgtcaca
    15241 acacccagat tcatgcgacc tgaattgact tctacaccat tttcatcctt ccatccttgg
    15301 aggaaagaac ggcaacccat aggcaccttg aaggatcctg tcaagtcaac aatcttatcg
    15361 taagataaaa catctggata catccgtttg gttgcacact caagagccaa ctgcttgatg
    15421 tcgtagttgg gagttccttc ctctaaatta aggcctcttt tcagagtgaa aataagttta
    15481 ggaaagatag ccgtacggtg ttctgaacca agacccttga tgcgaatgtt taaaatagct
    15541 ttttgaattt ctcgttcaaa acgactggtt cccagaccaa aacctagcga agtaaaaggt
    15601 gtttgtccat ttgaagtgaa gagagtattg atttcatact caagagattg catggcatcg
    15661 tagatgtcct tttgcgtttt cttccaagcg taatcttccc gtttttcagg caatacccac
    15721 tcttctgcat ctttgagatg tttttgatag ttcttctctg cataaggcgc caaaatttca
    15781 tcgatacggt cagctgaaca gccaccgtac tggctagaag caacgttggc aatgatttga
    15841 gaaatctgtg ctgtcgcagt ctggatagac ttgggactct ctacctctgc atttccaatc
    15901 ttaaaaccat tttccaacat acccttaaaa tcaatcaaac agcagttggt cataggggta
    15961 tagggactgt agtccaaatc gtgatagtgg atatccccct tttggtgggc attggctacg
    16021 tgcttaggaa gcatttgcag tccgattgat ttcccaacaa tccctgctgt caaatcacgc
    16081 tgagtgttaa agacatcact gtctttatta gcattttcat tgacaactgt ctggtctttg
    16141 ttgagaagtt tatgaatact aaagttgata tccgtcgctt ttgagcgctc aaaatccctc
    16201 tgtgtccgat aagtaatata ctcctcagcc agcgcatatt ctttggcttc aaggagttca
    16261 tgttctacga tattttgaat ttcgtaaatc ttaattccct gtggaaagcg actatgaatt
    16321 tctgtaataa ttctctcagt cagatcattg aggcgttttt caaccagggg tgtcacatcc
    16381 ataaccttgt cagctgcctt gtggagagcc ttgtcaatct tgtccacatc aaatacaaca
    16441 cgtctcccat ctcgtttctc gacgaagaga gttggcaaaa ttgtaatttt ttcttctagt
    16501 gcaatcatat gcatgctctt ttctaaaatg ttctttctaa aaagtattat accactctaa
    16561 aaagaaaaat caatatcttg tgttaaaaat cctaaaattt ttaaaaatac acaagatatt
    16621 gatttggtta tttaatttta tagactttaa actctgagta atcagtcttt acttgttggt
    16681 aattttcttt taaaagtgtt tccacttcag accagactac caccttgtca tttaccacaa
    16741 tcaccttagg ttgtttttct ttcaagtcat taagtaactt attacgattt tcctcagttg
    16801 ctgtatagtg caacggggac aaaatcgccg atggcgacaa gcgctcactc ttacgataaa
    16861 gagtcgcagt atcatcccat gcatagatag catcttccgt atttgtctcc tcttttacca
    16921 aatcagcaag acgattacgt tcttgataag gtactggata aaggacaaac ttcatcaaaa
    16981 aaggaacaga caaaagatag acaaacccaa aaactagcag atatagattt cctttgaaga
    17041 aacgactcca taggctaact ggcgtttctc ttcggcttct tctagcacta cgccccctct
    17101 gttgactctt gatattggtt accaataaaa gtaggaaaac agggaagacg accatcaaat
    17161 aagaagcgtg cagaggctct tgtgaaaaga aaagtaaaat caaacctaaa attaccacaa
    17221 gacttgcagg aaccgaaatg acgtatagtt taaagggttt ggattgaaag agacctaaaa
    17281 agatacaaac cacaaatccc aaaccaaggg acaacaagcc ataaaaaaac atattctcta
    17341 aaattttaga agtaaaatca aagcgaatac tgtcaatagg ataacgaata ccactaatcg
    17401 catccccaaa acttcctgtt gcagcactat agtaggcagt tggataaaag acaagtgaaa
    17461 aacctaaagc cactgcaaga aactgataaa acccatgcgc aaagcgtcta tgcccaaggt
    17521 taaagaccaa caagcctaaa ctcactacag caataaacag gagtgatgat aagggagcaa
    17581 agaaaaatcc gcctgccaaa gctaacccaa tccgtacaaa tcccttatca tggcttggat
    17641 tgcttaggta agccgcaact aaactaaagg ctgcgaataa gaaaggaagc gctaaaagag
    17701 tcgcatagcc tccaccaaaa gcaagacctg taactagcat gtaaaaaata gtcaccagat
    17761 gtccagcttg gtctccttgc tctgtcaagg tgtccgctga tctaaaaagg aaaaatcctc
    17821 ctgctaccaa ggctaaccac tcaaagatgg caaagaaaaa tccgccctga ctcacgtaag
    17881 tcagcaaata ataaagcaaa ccttgacttc cataatagtc gctgtaaatc ttccctgtct
    17941 gatgaagcgc ccaacctgca taaaaatcct gcacttcttg tgcactcatt aagtcgagta
    18001 atagcggtac tcctagagtt atccccgtta caagcgtact ccatagtaaa attttcacca
    18061 aaggaagacg acttgattca cgatgatgcg attcttgctc gatttggtat tctagaggtt
    18121 cacgattctc cttatgaact tcttctactc taccatacac actcatatcg tttctcctgt
    18181 tcaatttatc tgtcttagta tatcaaaaag tcctaggatt gtcagcttgc gatggctgtt
    18241 tgagtgattt tttgcatagt ttcacaaagg tttcaatttc ccgaaagcgg tgtaaatgtg
    18301 tctgtttgaa ctcaatcata tcattcactt cttccttagt tagcatgaat tcccctcctc
    18361 tttctttcta ttcgtaaaag acaagaaaaa taggtcgggt ttgtatccta agttctagat
    18421 ttcttataag ataggggcga atagctgggc gatttctttt acaagctttt gataccagct
    18481 cgttttgata gaatgaggat agacttcctg agaatctgca aatatatttc gaaaatctcg
    18541 ggcgatttcc cttatagaag gagttttata gagtaagact gcattttcat aatggtgtac
    18601 caagctccgg tagtcgagat tgattgtccc caccaccgca aaatcttcgt ctaccaacat
    18661 ctgcttacta tgaataaaac ctggactata ctcataaatc cgaacaccag cagaaagaag
    18721 gtcgggataa gctcctctcg tgactaactg aatgaacttc ttatctggta tgtaaggggt
    18781 gataattcga acatcgaccc ctctcatagc tgcatttttg attgtctctg ttaaatcata
    18841 atctataatc aaataaggtg tcgtaatata gaccgattcc gttgcttgat tgattaaact
    18901 ctgataaact tttttcccta cctgcgctcg aaaaattggc ttgggtccac ttccgtatgg
    18961 aatggttaaa ccgtcactcg ggatagaatg attttctaaa tgatattgat caaaatcact
    19021 aatttctcct cgattaatgt accaagtggt caaaaataag cgtgtcaggg cttttactgc
    19081 tagtccgtct aagcgaattc cactatcctt ccaataacca aatctctcga cgtggttaat
    19141 gtactcatct gccagattga ccccaccagt ataggctatc tgaccatcaa caatcaatat
    19201 ttttctatga tctctgttat tataagccac tgtcaaacga ggaataactt tattgaattt
    19261 atgggcctcg atgcccagct gacgaagtcg atgtgcataa tctcctgtta aagtagccat
    19321 acagccgata tcatcataga gcatcttaac ctctacaccc tgagctacct tttgctctag
    19381 tatatctagt atgcgattcc acatcaaacc ttcttctata atgtaatatt ccaagaaaat
    19441 aaatttctca gcctttttaa gatcttctac catctttttc cacatagctt ctccgttagg
    19501 aaaaaatgta gaggcagttt gatcatagat gtcagcattg gtatccatac tcaataagga
    19561 cttgatgact ccataagctg ccttgtcact ttcttttaat ttctctttta gtagctggct
    19621 atttgcttct tggaaatgca tagagcctag cttcttcagt tgtttgattt cttttttgga
    19681 caatcgcctt tcaccaaaca tcagatagag taagggacca aatactggca caaaggctac
    19741 taacaaccag gttaccttat tctcaggagt cgtattacgg ttgactatcg aaatgattgt
    19801 gatgatactc agtagaatga ggacagtaat ccataaaatg ggagccatgc gtcctaaata
    19861 aagaaagaga ccaaaaacaa gacaaagttc aagtagcata atcgaaagac taaagccata
    19921 cttggacatc aataattgaa atttcctata tttcatatcc cctccttgat agtttgaatg
    19981 ctaaaaacaa gtaattgata ctagtataac gcaggtattc ggtagaaggc aagtatttat
    20041 gattattttc taggtaaaag taaaaaggct gaaaaatcca gccctttcta cttgcatagt
    20101 cttatttagt taaaacgatt tggtccgatg ggtctctatc catatcatct acgacgattt
    20161 gattaccgtt tacagtgtaa attttgacat catcgccaat aatcatgcgt tgatttgctg
    20221 aatcaaatgt aacctgtttg actttctgat ccccgtcaga ttcgagctca gtccatgtac
    20281 cagtcgttcc agttaccact aaagtgatac ggtctccgtc atcctgacca gtataagtcc
    20341 catcaatatc ggtcggttga gcaacaggtt ggttttgtac tggagccgct tgattttggt
    20401 tacctcctgc atttgcagta ttggtattct gttgagcagg ttgttgagca ggctgttgga
    20461 cctgctgctg agcaacaggc tgttgaacct gttgagctct ttgtgaacaa gctactaaga
    20521 gactaaaact tgctagagaa gcaagagaaa gtgtgaatct ttttaatttc atgatgaatt
    20581 tcctttctgc taccaattta gagaaatttt ctctaaccag caattcccct agtataacaa
    20641 gttcaaacaa gggggtcaat ttatctgctc acggtccagc aggtagcccc gtacttctga
    20701 gataaaatag agagaccctg taacgaacag caagtcttga gcgtctgccc tttcttcgaa
    20761 actgctgata aattctcggc aagaagaaac tatatcgtaa cctgtcacat ccctttcgtc
    20821 caaagccccc tgatagtcaa agccggtcac cttgagttcc acctgaggca atttttcagt
    20881 caggtaacct aacatccctt gataatcctt ccgtttcaag gctccaaaaa ggatttgagg
    20941 tcgatagcct tcctgctctt tttctttgat aaactcaacc aagcgagtca aggcagggag
    21001 gttatgagca ccatccaagt aaatctgtgg gcgaatacgc tccaagcgac cagcccaatg
    21061 ggtctgttcc aaggcctttc ttacagcctg ttcatcaaca gcttcctttc cttctcccat
    21121 aaaaagaaga aaagtttgca acgccaaggc tgcattctcc tgctgataga ctccttctaa
    21181 gcctatgttc agctgtgaaa aatttagtaa agagcttgaa aaatcaccat tcagcattaa
    21241 aaaatcttga cctgcctgat aaaggttaac agctaaagat tcggcttttt tctgacagac
    21301 aagcctagct tctggaggca atttcgcaat cactgccttc ttaccagcct tgaaaatacc
    21361 agctttctgc tctgcaattg cttctagact atcacccaag gtttcttgat ggtcaagccc
    21421 aatggaggtg atgacagcaa gctctccagt taccacattg gtcgtgtcaa gtaagccacc
    21481 aattcccact tctaataaaa ccaaatccac ctcttgctcc ctaaaataaa gaaaagcaac
    21541 caaggtcagc atttcaaaaa aggacaactg gtcaggagtt tgcagaagcg ttttctccat
    21601 ctccttgacc tgatcagcca aacggataaa gtctgcgtct gctataggtt gcccattaat
    21661 gcagattcgg tcattgatag agacgatatg aggggaggta aaggtcgcaa cttttttgcc
    21721 atgccccata aataattccc tcataaaagc aatggtagat cctttcccat tagtccctgt
    21781 tacgtggata atagggtaag actgctcagg atttcctaac aaatccaccg cttgctgtat
    21841 tcggcccaaa cctgatcgaa aattcaaacc aatccgacta tgaagccatt cttctacttc
    21901 aaacatacac atctccttga caaaagtcca atcaattatc gcattaaagt atggttacta
    21961 ataaaaacaa ggccaggatt ttcgtcccga cctcttacct ggttagctaa taactagcca
    22021 ctatgaatgt gaatatgggc taaaaacatc cactagatgt tccaactctt ccccatttct
    22081 gggagttggg ctgatacagt ctcccagact gtatcactcc tccataaagc tgttgaagac
    22141 ttcttcaatc atgttccatt cgtcttctga gtcttctggg attggttgca attcgccttc
    22201 tgttccatct tcattttcaa tgaatgagta agcttggatt tcaacttgtc cgtcttcgtc
    22261 ttcttctgcg ttaactggta ctagaagaac atagttttta ccaaattctt cttttccatc
    22321 aattgtcaaa aggatttcaa acaaggtttc atttccttgc tcatctacta gtgtgattag
    22381 ttcacgttct tcgtggtcgt ggttatgatc gtgtgacata gcctctcctt tatattaaaa
    22441 ttttctatct aaataatttt gtaaaatcag ctgagctgct aacttatcaa tgactttctt
    22501 gcgcttattg cgactgatat ctgcttgttc aatcaacatg cgctcagcag ccactgttgt
    22561 caagcgttca tcctgatagt ctaatggtaa accaaaaaac tcttctagct ttgctccgta
    22621 ggcttgacta gcttctacgc gcggtccact tgtattgttc atgtttttag gcaagcccac
    22681 tacaaatcgt tccaccttgt aagtatcaac caattcctta acgcggtcaa aaccaaattg
    22741 gccttgttct tcatttatct ggatgatttc aagcccttga gctgtaaaac caagcggatc
    22801 gctaatcgcc acccctaccg tttttgaacc gacgtccaat cccataattc tcataggtta
    22861 tagatcgact ccttgtcctt tgaggtagta gcgaaccaat tcctcaacga tttcatcacg
    22921 ctcatactta cggatttgat ttcgtgcatt attataacga ggaacgtagg cagggtctcc
    22981 actcaatacg taacctacga tttggttaat tgggttgtaa cccttatcgt tcaacgaagc
    23041 ataaacatct gtcaaagttt cgctaatttc ttttttattg gaatcgtcca atttaaaacg
    23101 tactgtttct tcagtaaatc ccattctaac accctctttc cttagaatag taccattata
    23161 gcataattcc ttaccttcta caattcaggc agtctattta tttggatttt ctattgttct
    23221 gtcgcgccat ttgccaatct atctgaaata tatttgcttg gttcattttt caaaagattt
    23281 tccaaaccaa tattcttcag atgttccaac tgggaagcct tcttgacatc cagaacttga
    23341 aaatcaaaac tagtcgttgt ttgaagttcc gttgcgctca atagttttgt ttcaagtttg
    23401 aaacctgcca atttacgagc ttcaatgata gacttatcct tctcctccgc ttcaagaaga
    23461 gctttttgag tttcctccac tccatgttgg tcaatataag tcttcaactc atcagagact
    23521 ggacgttttt gttggatagt tagactcaaa aaagtcttgt ccttgtatgt aatggtttgt
    23581 gtctgctggc taccatcatc tgacttgggc aagaccaaag tcttagttac aactgtattc
    23641 ttctcagcat tttcaataac tggcaatgcc gactgaagcg tatctttttc tgtttttgta
    23701 gctggtccag tttctttttt ctgtccgcaa ccaaccagga caaaaaggaa agctagacta
    23761 acaagaacta tttttttcat ttctttcttc tttctttttg aaattaaaat agaataagac
    23821 tgggaagtgc tcccagcctt gatgtttata gagctgcacg caaacgtgct tctgcatttt
    23881 ctacattacg gacagagcgt ggtaggaagg cacgaatatc gtcttccttg tagccaactt
    23941 gcaggcgttt ttcatctaca aggattgggc tctttaaaat tctcggtgtt tccataatca
    24001 gattgagaac ttcattgaca ctcaaatctt caatatccac tccaagggct ttggcatagc
    24061 gatttttaga cgaaacgatg ctggctattc cgttatctgt tttggttaga atatccagta
    24121 attcttctct cgtaattcct tctttaccaa ggttttgttc tttataactt aactggtggg
    24181 cattgagcca ggtttttgct tttttacagc tagtacaact tgagactgta taaattttaa
    24241 tcatgtacct acccctttcg ctacatgtta ctatcagttt agtctattat accataaaaa
    24301 acatccgact tgcgacctat ttttaat
//
LOCUS       DAVBBS010000029        24255 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVBBS010000029 DAVBBS010000000
VERSION     DAVBBS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24255
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.029"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..338
                     /locus_tag="VQU32_001151"
     CDS             <1..338
                     /locus_tag="VQU32_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2472614.1"
                     /translation="AGLTNGELIAPMTYEEMMTSDFFEAWFQKFLLPTLTTPSAIIMD
                     NARFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAHIKKHLKKVLPSCCNTFYE
                     ALLSCSCFN"
     gene            complement(545..2803)
                     /locus_tag="VQU32_001152"
     CDS             complement(545..2803)
                     /locus_tag="VQU32_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2472615.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(3032..3262)
                     /locus_tag="VQU32_001153"
     CDS             complement(3032..3262)
                     /locus_tag="VQU32_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HET2472616.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3452..4135
                     /locus_tag="VQU32_001154"
     CDS             3452..4135
                     /locus_tag="VQU32_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2472617.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSEIVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            4135..4869
                     /locus_tag="VQU32_001155"
     CDS             4135..4869
                     /locus_tag="VQU32_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2472618.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDIAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            5023..5880
                     /locus_tag="VQU32_001156"
     CDS             5023..5880
                     /locus_tag="VQU32_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HET2472619.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGYRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            6022..6894
                     /locus_tag="VQU32_001157"
     CDS             6022..6894
                     /locus_tag="VQU32_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET2472620.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLMTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVILLGPGL
                     GDNAFGEDLVKQVFAGLKQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            7176..7859
                     /gene="rpiA"
                     /locus_tag="VQU32_001158"
     CDS             7176..7859
                     /gene="rpiA"
                     /locus_tag="VQU32_001158"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET2472621.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7873..9084
                     /locus_tag="VQU32_001159"
     CDS             7873..9084
                     /locus_tag="VQU32_001159"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HET2472622.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            9086..9628
                     /locus_tag="VQU32_001160"
     CDS             9086..9628
                     /locus_tag="VQU32_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET2472623.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            9644..10453
                     /locus_tag="VQU32_001161"
     CDS             9644..10453
                     /locus_tag="VQU32_001161"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET2472624.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10929..11909
                     /locus_tag="VQU32_001162"
                     /pseudo
     CDS             10929..11909
                     /locus_tag="VQU32_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
     gene            11934..12446
                     /locus_tag="VQU32_001163"
     CDS             11934..12446
                     /locus_tag="VQU32_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HET2472625.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPISAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            13093..13803
                     /gene="deoD"
                     /locus_tag="VQU32_001164"
     CDS             13093..13803
                     /gene="deoD"
                     /locus_tag="VQU32_001164"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET2472626.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(13826..14350)
                     /locus_tag="VQU32_001165"
     CDS             complement(13826..14350)
                     /locus_tag="VQU32_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HET2472627.1"
                     /translation="MKIAIIGYSGSGKSTIAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(14403..14639)
                     /locus_tag="VQU32_001166"
     CDS             complement(14403..14639)
                     /locus_tag="VQU32_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET2472628.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(14707..15627)
                     /locus_tag="VQU32_001167"
     CDS             complement(14707..15627)
                     /locus_tag="VQU32_001167"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HET2472629.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            15817..16221
                     /locus_tag="VQU32_001168"
     CDS             15817..16221
                     /locus_tag="VQU32_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472630.1"
                     /translation="MKILKRYILELCFILSFALPFIKGTNADNGRCFVETYYGFTFLM
                     EHAIVTAVFIYSFLIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLIIKYFKVKQ"
     gene            16437..17018
                     /locus_tag="VQU32_001169"
     CDS             16437..17018
                     /locus_tag="VQU32_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2472631.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGQKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            17015..18292
                     /locus_tag="VQU32_001170"
     CDS             17015..18292
                     /locus_tag="VQU32_001170"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HET2472632.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIRGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVHQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            18310..18972
                     /gene="deoC"
                     /locus_tag="VQU32_001171"
     CDS             18310..18972
                     /gene="deoC"
                     /locus_tag="VQU32_001171"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET2472633.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            18959..19348
                     /locus_tag="VQU32_001172"
     CDS             18959..19348
                     /locus_tag="VQU32_001172"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET2472634.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            19438..20490
                     /locus_tag="VQU32_001173"
     CDS             19438..20490
                     /locus_tag="VQU32_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HET2472635.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALNNAVKDAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            20634..22169
                     /locus_tag="VQU32_001174"
     CDS             20634..22169
                     /locus_tag="VQU32_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2472636.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            22162..23220
                     /locus_tag="VQU32_001175"
     CDS             22162..23220
                     /locus_tag="VQU32_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2472637.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            23223..24179
                     /locus_tag="VQU32_001176"
     CDS             23223..24179
                     /locus_tag="VQU32_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2472638.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         7049 a         4388 c         5514 g         7304 t
ORIGIN      
        1 ttgcaggtct aacaaatggt gagttaatcg ctccaatgac ttacgaagag atgatgacga
       61 gcgacttttt tgaagcatgg tttcagaagt ttctcttacc aacattaacc acaccatcgg
      121 ctattattat ggataatgca agattccata gaatggggaa gctagaactt ttatgcgaag
      181 agtttgggca taaactttta cctcttcctc cctactcacc tgagtacaat cctattgaga
      241 aaacatgggc tcatatcaaa aagcacctca aaaaggtatt accaagttgt tgcaatacct
      301 tttacgaggc tcttttgtcc tgctcttgtt tcaattgact atagcagatt gaaataagat
      361 gagaacaaat cgattgggaa agtaaaatta atttctataa atgttttagc aattgtttcg
      421 tactatttca gattcagtct actatatatc ataatggaca aagggcaagc tgtcaagaaa
      481 ttacagttat tttagtaaaa aaagaagcag aaaaatttaa ttttctactc cttttctata
      541 ggatttattt ttctttttca gaacttttaa cttcagcttt tttggcagat ttaatctgaa
      601 tgtttccctt gctagtcata actgctttga gatctttttc gcttggattt tcaaggtagt
      661 agtcagtgat tgtgtcctca atatagtctt gaatagtccg acgaagtggg cgtgctccca
      721 tttttggatc ataacctagg tcaaccaact tttccttgac cttgtcagtt acatccaaac
      781 gaatgttgtt gctagagagg cgcttgttaa catctgctag catgagctcg acaatctgaa
      841 ggaggttatc cttgctgaga gccttaaatt cgataatgcc atcaaaacgg ttcataaact
      901 ctgggctaaa gaagttaccg agttcaccga ggacagaatt ggtacgtcct tctctagcag
      961 caccaaatcc aacgctagct tcggtctttc ctgtacctgc atttgaggtc atgataatga
     1021 tggcatcctt gaagctaacg gtgcgtcctt gcccgtctgt caaacgacca tcgtccaaga
     1081 cttgaagaaa catgtgcata acatctgggt gagctttttc cacttcatcg agaaggatga
     1141 gagaatatgg attgtggcga actttttcag ttaattgacc agcctcatca tagccaacat
     1201 aacctggagg agcgccgacc aacttagcca cactatgttt ttccatgtat tcactcatat
     1261 caaagcgaat catactatca gcagaaccaa aaagttcgat agccagttgt ttggaaagtt
     1321 ctgtcttacc gacaccagtt ggcccaacga agaggaagct tccgattggg cggttagggg
     1381 taccaagtcc gacacgatta cggcgaatag ccttggcaat cttatcgact gcatcatctt
     1441 ggccaataac atgagacttg agatcttcgg ctagatggat gagttgagat tgttctttct
     1501 ctttcaaatc accaacaggg atattggttt tctgctcgat aatgtgctca atagttttct
     1561 cgctgatgat aggagtatcc tggtctgtga tctttttctt ttgcatttcc ttatacttgg
     1621 caatctggtc gcggaagtag gccgccttct caaaatcttc ttctcgtgta gcttgagact
     1681 tgagattttc agcctcaatc aagcgctgat caattacttt aggatccaca aaattcaagg
     1741 tcaagttcat cttagaacca gcttcatcta ggaggtcaat ggccttgtca ggcaagaagc
     1801 gatcttggat gtagcgattg gaaagagttg cagctgcttc aatcgcagca tctgtatatt
     1861 gaacgtggtg gtaatcttcg tatttctttt gaatcccttt gagaatagtg attgtttcgt
     1921 ccaccgttgg ttcatcgact ttaacaggct gcatacgacg ctcgagggca gcatcctttt
     1981 caatgatacg gtattcattg agggtagtag caccgactag ttgcagttct ccacgagcaa
     2041 gggctggctt gaggatattt cctgcgtcca tattaccatc actcgcagaa ccagcaccaa
     2101 caatttcatg gatttcatcg ataaagagga tgatgtcttc acgtttgcga atttcttcca
     2161 tgagtttttg catgcgttct tcaaattgtc ctcgaatccc cgttccttga actaagctaa
     2221 ccacatccag acggatgact tgtttacctt ggagtttatg tggcacatcg ccatcgacaa
     2281 ttttctgagc tagaccttcg acaacggccg tttttccgac accaggttca ccgataagga
     2341 caggattatt cttggttcta cgattgagaa tctcgatgac acggataatc tcatcgtcgc
     2401 gcccaataac ggggtcaatg tctccacgac gggcaatttc agttacatta ataccaaatt
     2461 cttccagcag gcctttttct tggctaggtg gcggagtttg agcagatcca cgattttggg
     2521 aaccataacc gccgtttcca ccgtaacctc cacctgattg ggttggggga ataggaggag
     2581 tattgctaga aggtctgaaa ttgtttagat cattgaagaa atcaccaaag ggatcgaagt
     2641 cacgattgtt cagatccgtc atacctttga agaggctatt gttaggatct gttttgataa
     2701 tcttatagca gttttgacag aggtcaattt gtttttgttt tccattgaga ttggtgtaaa
     2761 gatgaattgt tgagtcgtta attttacagt tttgacaaag catacatcta cctcatttct
     2821 ttctttagcc tgacttgttt aaaggtcaaa aatagtcaaa ttctatactc tcattataac
     2881 aggattgggg tgtaaagcaa gtaaaaagat tgaagctttg agaagttgtt acaatgaaaa
     2941 tttttgtgaa tttggactat aaaaaaatcc tatttgtgag acaaaaagga ttttacttag
     3001 acatgcaggg acaatcccag cttgttttag attagtagag aagttcgtaa atttccattg
     3061 cgattaagtc aatgctatca aacgtatacg tttcgcgatc ttctacgtca cgaagggtaa
     3121 agattgggcc gttttcttcg tcgtggctgt acgcaacttc tgcaacaaca actccttcgc
     3181 gttcaaaatt acgttttaaa tttccgccat cgtttgccat tgcttcaagg cggttgatga
     3241 ttctaaccaa atgtgattcc attttgattc tccttcattt cttatcgtta ctattttacc
     3301 ataaaggagg ccaacttgac tagaaaaaac taagatttct cgctaattct gctagtttaa
     3361 agatttttaa ataaattgga taaaaaattt aaattttaat tcaaaacatg ttataatcat
     3421 acagtattct attttagaaa gcagtgtgac tatgaatttt tcttttttac ctaagtattt
     3481 accttatttt aactatgggg ctgttgtgac gattcttatt tctatctgtg ttatcttttt
     3541 gggaactatt ttgggtgttg tcttggcttt tgggcaacgt tcaaagttta aaccgcttgt
     3601 ttggttggcc aacttgtacg tttggatttt ccgtgggaca ccgatgatgg ttcaaattat
     3661 gattgccttt gctcttatgc atatcaatgc tccgactatt cagattggaa ttttaggtgt
     3721 tgatttttcg cgtctgattc cagggatttt gattatctct atgaatagtg gtgcttatgt
     3781 ttcggagatt gttcgtgccg gaatcaatgc ggttccaaaa ggtcagctag aggcggctta
     3841 ttcgctaggg attcgtccta aaaatgcgat gcgttatgtg attttgccac aagcagtcaa
     3901 aaatatcttg ccagcattgg ggaacgaatt tatcaccatt atcaaggaca gctccctctt
     3961 atcagctatt ggggtcatgg agttgtggaa tggggctaca acagtttcta caacaaccta
     4021 tctaccttta acaccacttt tatttgcagc attttactac ttgattatga cctctattct
     4081 gacagtagcc ttgaaagctt ttgaaaaaca tatgggacaa ggagataaga aataatgaca
     4141 gaaaccttga taaaaattga aaatttacat aaatcttttg gaaagaatga agtattgaag
     4201 ggcatcaacc tcgagattaa aagaggagaa gttgtcgtta tcatcggtcc ttcagggagt
     4261 gggaaatcta ccttgcttcg ctctatgaat ttgttggagg aagcaaccaa ggggaaggtt
     4321 atctttgagg gagtcgatat tacggacaag aagaatgacc tgtttgccat gcgtgagaag
     4381 atgggcatgg ttttccaaca atttaacctc tttcctaata tgactgtgat ggaaaatatt
     4441 accttgtcac ctatcaagac taaaggtgaa agcagggaag ttgctgagaa gagagcccaa
     4501 gagcttttgg aaaaagttgg tttgccagat aaggcagacg cttatccaca gagtctgtcg
     4561 ggtgggcaac aacaacggat tgccatcgcg cgtgggttgg ctatggaacc agatgttttg
     4621 ctctttgacg aaccaacttc agccttggat cctgaaatgg taggtgaggt attggctgtt
     4681 atgcaagaca ttgccaagtc agggatgact atggttatcg taacacatga gatgggattt
     4741 gcccgtgagg tggcagatcg tgttatcttt atggcagacg gtgtggttgt tgaagatggg
     4801 acacctgagc agatttttga acaaacccaa gaacaacgga ctaaagattt cttgagtaag
     4861 gttttataat ctattttaaa atttcaagac aagattagtg aaaagttcaa ccagagcttt
     4921 ttcttatagt ttgaaactat aggataacct aggaaagaag tgttagaggg cactagcagt
     4981 ttttggtata atggaagata tttgaaagaa aagagaagtg atatgacaca gattattgat
     5041 gggaaagctt tagcggccaa attgcagggg cagttggctg aaaagactgc aaaattaaag
     5101 gaggaaacag ctctagtgcc tggtttggta gtgattttgg ttggggataa tccagccagc
     5161 caagtctacg ttcgcaacaa ggagaggtca gctcttgcgg ctggttaccg tagcgaagta
     5221 gtgcgagttc cagagaccat tactcaagag gaattgttag acctgattgc taaatataat
     5281 caggatccag cttggcatgg gattttggtc cagttgccat taccaaaaca tatcgatgaa
     5341 gaggcggttt tattagccat tgacccagaa aaggatgtgg atggtttcca tcccctaaac
     5401 atgggacgtc tttggtctgg acatccagtc atgattccct cgacacctgc aggaattatg
     5461 gaaatgtttc atgaatatgg gattgacttg gaaggtaaaa atgcggtcgt aatcggtcgt
     5521 tcaaatatcg ttggaaaacc tatggctcag cttcttttgg ctaaaaatgc gacagtaacc
     5581 ttgacccact cacgaactca taatcttgcc aaggtggctg ctaaagcaga tattcttgta
     5641 gtcgcaatcg gccgcgccaa gtttgtgact gctgactttg tcaaaccagg tgcggttgtc
     5701 attgacgttg ggatgaatcg agatgaaaat gggaagctct gtggagatgt tgattatgag
     5761 gcagttgcac cacttgctag ccatatcacg ccagtccctg gaggtgtcgg tcctatgacc
     5821 attaccatgc tgatggagca aacttatcaa gcagcacttc ggacattgga tagaaaataa
     5881 gagaagcatt cgattaatag agtgtatttt ctatagctat atctaaaatg gcagaaatga
     5941 atattaaatt ttagatataa gattacaaaa ggaggtctgc gtctcctttt tattgtataa
     6001 taaaagtgag aggaaaaaag gatgaaagtg attaatcaaa ccttattaga aaaagttatt
     6061 attgaacgtt ctcgttcaag tcataaagga gattatggtc gtctgctgct gcttggtggt
     6121 acttatcctt atggaggtgc catcatcatg gctgctttag cagctgtaaa aagcggtgca
     6181 ggattgatga ccgttggaac ggacagggaa aatatcccgg ctctgcacag ccatttacct
     6241 gagcctatgg ccttttctct tcaagaccag caattgttaa aagagcaatt ggagaaggca
     6301 gaagttatct tgctggggcc tggtttagga gacaatgctt ttggagaaga tttagttaaa
     6361 caggtctttg ctggcttaaa acagaatcag attttgattg tagacggagg tgccttgacc
     6421 attcttgcta ggacaagttt gtcatttccc tcaagccagc ttatcctaac tcctcaccaa
     6481 aaagaatggg aaaaattatc tggaattacg attgaaaagc aaaaggaaga tgcaacggct
     6541 agtgttctga cttccttccc tcaaggaaca attttggtgg agaaaggccc agctactcgt
     6601 atttgggaag ttggccaatc tgattattac cagttacagg ttggtggtcc ttatcaggcg
     6661 actgggggaa tgggagatac tctggctggg atgatcgcag gatttgtagg ccaattccga
     6721 caggccagtc tctacgagcg tgtggcagta gcgacccatc ttcattcagc catagcccaa
     6781 gaactatctc aagaaaatta tgtggtctta ccgacggaaa tcagcaggta tcttcccaaa
     6841 ataatgaaaa ttatttgtca acaagagagg gtaagcaaag ataaacttgt ttaaaataga
     6901 cagataaatc tacttccttc tcttaattta tgattcttga aatgcgcctg ctttaaggct
     6961 aaactagacg tatcaaagtt aagagtttgt ttttgtaact ggaaagagat gaacaaatca
     7021 aatacataaa agataaactc atttttattc gttttgtaaa aataaaatgc atatttttaa
     7081 agaaagattg atgaaaaatg tagatttgat gtttttactt gaataaaaag cggtttcata
     7141 gtaaaataag aactgagaat gatggaggta aatcggtgga aaatctgaag aaaatggcag
     7201 gtatcaaggc tgctgagttc gtgagcgatg gaatggtcgt tggacttgga acaggctcga
     7261 ctgcctatta ttttgtcgaa gaaatcggtc gtcgtatcaa ggaagaagga ttgcagatta
     7321 cagctgtaac aacttctagt gtgaccacta aacaggcaga aggccttaat atcccgctca
     7381 agtctattga ccaagtagac tttgtcgatg tgacggttga tggcgcggat gaagtggata
     7441 gtcagtttaa tggaatcaaa ggcggtggtg gtgcccttct catggaaaag gtggtcgcaa
     7501 caccatcaaa agaatacatt tgggtggtgg atgaaagcaa gctggtcgaa aaactaggtg
     7561 cttttaaatt gccagtagaa gtggttcagt atggtgcaga gcaggtcttt cgtcattttg
     7621 aacgagctgg ctacaaacca agtttccgtg aaaaagacgg tcaacgtttt gtgacggata
     7681 tgcagaattt tatcattgac ctcgccttgg atgtcattga aaatccaatt gcttttggac
     7741 aagaattgga ccatgtcgtt ggtgttgtgg agcatggttt attcaaccaa atggtggata
     7801 aggtaatcgt tgctggacga gatggagttc agatttcaac ttcaaaaaaa ggaaaataga
     7861 agggggcata agatgtctaa atttaatcgt attcatttgg tggtactgga ttctgtagga
     7921 atcggtgcag caccagatgc taataacttt gtcaatgcag gggttccaga tggagcttct
     7981 gacacactgg gacacatttc aaaaacagtt ggtttgaatg tcccaaacat ggctaaaata
     8041 ggtcttggaa atattcctcg tgaaactcct cttaagactg tagcagctga aagcaatcca
     8101 actggatatg caacaaaatt agaggaagta tctcttggta aggatactat gactggacac
     8161 tgggaaatca tgggactcaa cattactgag cctttcgata ctttctggaa cggattccca
     8221 gaagaaatcc tgacaaaaat cgaagaattc tcaggacgca aggttattcg tgaagccaac
     8281 aaaccttatt caggaacggc tgttatcgat gattttggac cacgtcagat ggaaactgga
     8341 gagttgatta tctatacttc agctgaccct gttttgcaga ttgctgccca cgaagacatt
     8401 attcctttgg atgaattgta ccgtatctgt gaatacgctc gttcgattac ccttgagcgt
     8461 cctgcccttc ttggtcgcat cattgctcgc ccttatgtag gtgaaccagg taacttcact
     8521 cgtacggcaa accgtcgtga cttggctgta tctccatttt ccccaactgt tttggataaa
     8581 ttgaatgagg ctggtatcga tacttatgct gtgggtaaaa tcaacgatat ctttaacggt
     8641 gctggtatca accatgacat gggtcacaac aagtcaaata gtcatggaat tgatacacta
     8701 ttgaagacta tgggacttgc tgagtttgaa aaaggattct cattcacaaa cctagttgac
     8761 tttgatgccc tttacggcca tcgtcgtaat gctcacggtt accgtgattg cttgcatgag
     8821 tttgatgaac gcttacctga aattatcgca gctatgagag agaatgacct tctcttgatt
     8881 actgcggacc atggaaatga cccaacgtat gcaggaacgg atcacactcg ggaatatatt
     8941 ccattgttgg cctatagccc tgcctttaaa ggaaatggtc tcattccagt aggacatttt
     9001 gcagatattt cagcgactgt tgccgataac tttggtgtgg aaactgctat gattggggaa
     9061 agtttcttag ataaattggt ataagatgac gcgctatgct ttgctggtga gaggtatcaa
     9121 tgttggtggt aagaataagg tcgtcatggc ggagcttcgt caagaattga caaacttggg
     9181 actggaaaag gttgagagct acatcaatag tggcaatatt ttctttactt cgatagattc
     9241 caaagcccaa ttggttgaaa agctagagac tttctttgca gtccattatc catttattca
     9301 gagcttttct ttactgagtc tagaggactt tgaggcggaa cttgaaaatc taccagcttg
     9361 gtggagcaga gacttggcac gaaaagattt tctcttttac actgagggtt tggatgtgga
     9421 ccaagtcatc gcgacagttg aaagtttaga gctgaaagat gaagtgcttt attttggaaa
     9481 acttgggatt ttctggggga aattttctga agaatcctat tctaagactg cctatcataa
     9541 gtacttgctg aaggtgcctt tctaccgcca cattactatt cgtaatgcta aaacctttga
     9601 caaaattggt caaatgctaa aaaaataata aaggagacac acaatgacat ttttaaacaa
     9661 aatccatgaa actgctactt tcctgaaaga aaagggaatt gcagcccctg agttcggtct
     9721 aatccttgga tcaggacttg gagaattggc agaagaaatc gaaaatccag ttgtagtaga
     9781 ctatgctgag attccaaact ggggccgttc aacagtagtc ggtcatgctg gtaaattggt
     9841 atatggtgaa ctggcaggtc gcaaggtctt ggctcttcaa gggcgtttcc atttctatga
     9901 agggaatcct ctggaagtgg tgactttccc agttcgtgtg atgaaagttc ttggatgtga
     9961 aggtgttatt gtaaccaatg cagctggcgg tatcggattt ggtcctggta ccttgatggc
    10021 tatctcagac catatcaaca tgacggggca aaatccattg atgggtgaaa acttggatga
    10081 ctttggccca cgtttcccag atatgtctag ggcctacaca ccagaatacc gtgccactgc
    10141 ccatgaagtg gctaaaaaac ttaatatcaa gcttgatgaa ggtgtctata tcggagttac
    10201 tggtccgact tatgaaacac cagcagaaat tcgttcctat aagacactgg gagcagatgc
    10261 agttggtatg tctacggttc ctgaagttat cgtggcagcc cactctggct tgaaagttct
    10321 gggaatttca tgtatcacta actttgcggc cggtttccaa gaagaactca atcacgaaga
    10381 agttgtagaa gtgactgaac gtgttaaagg tgatttcaaa ggcttgctta aagcgattct
    10441 tgctgaattg taagaaaaaa gatttaaaag ggggagtgcc tctgtttttt caggattgac
    10501 tgcctatccg gattaaagaa gaaacagagg aatactatga gcttcttcct gctcttataa
    10561 ctgaaagaag cggaagaata ggtatgtctg atctgatagc cagcattgtg aaagacaaga
    10621 ttctaggata ctagcattag cttcctagcc aagcagacta gtatgataag gagagatgag
    10681 aatgaattga ctttctgaat ttctcagtct tatcatatat agcacaatga gatttcgctt
    10741 gagtctgctt gtaaataaac gaaaagaaag ataagaaata atgaaaattg gtcaacgaat
    10801 tatgcgcttt ggcataaaaa attaagtatc ggagttgtat ctgttgtagt cggctttgat
    10861 ttctagctcc agctggaatt tcagccaatg aagtaaagca agatgtaaca tctgaagtgg
    10921 taataggtgt gctagattct aaggaggaat tgaaagagtc agaaaatgat gctccaaaac
    10981 tagaaactcc tcttagagag gagccaagac tagctcctca aacgcttccg gaagcaagtg
    11041 aagttcttga aaacaaaagg gaagagtcaa aagtagagat aacataacca gctcaagcgg
    11101 atgatatccg caaggttgtt ggggaattag ccaaggatat aagtattact aagttgtata
    11161 tgacaggtca ttctcttgga tgttacctag ctcagattgc agcggttgaa gcttaccaaa
    11221 aatatcctga tttttataac catgtattga ggaaagtgac aactttcagt gctcctaaag
    11281 tgattacttc cagaactgtt tggaatgcta agaatggttt ctgggatgtt ggtttggaaa
    11341 gtcgtaaatt agctgttagt ggaaaaatta agcattatgt ggttgataat gacaatgttg
    11401 tgactccctt gattcataat aatcgtgata ttgttacatt tacaggtaat tcacgcttta
    11461 aacaccgttc tcgtggctat tttgaaagtc caatgaatga tattcctaac tttaatattg
    11521 gtaaacaagc taccttggat aaacatggtt atcgtgatcc gaaattggat aaagtgcgat
    11581 tctttaagaa acaggctctg cctcgatctt ctagtcaacc aagcgctgaa ccaatggaaa
    11641 atattgcctt aggaaaacag gttactcaaa gttcgacagc tttcggagga gatgctagaa
    11701 gagctgtgga tggcaaagtc gatggtaact atggtcacaa ttctgtcact catacaaact
    11761 tccaatctaa gccttggtgg caagtagatt tggctaaaga agaaaccatt cgccaaatca
    11821 atatttacaa ccgaacagac actgcccagg atagattggc aaactttgat gtcattcttt
    11881 tagacagttc tggtaaagaa attgagtgaa aacgtataat atctcctaaa gatgtgtcag
    11941 cacaaattac gattaaccat aaaaaagcgc gctatgttcg gattgagcta gaaggctata
    12001 atgccctcag tcttgcagaa gttgaagttt tccgctttat agctacgaat gctgaaacgg
    12061 cgacacaagt ttctaagcca gttcaaccaa tcagtcagac tcctgtgaaa gataaaacat
    12121 tgacaattca acacagtgga gcttacattg cccgctactc cataacttgg gaagaagttc
    12181 cagtagataa agatggaaac caagttgttc gtagtcattc ttgggaagga aacggtcgca
    12241 accagactgc gggttttgtc ctcaacctcc caatcaaaga aaatatgaga aatctgcgag
    12301 ttaagattga gaaaaagacg ggcctactat ggaatagatg gcaaacaatc tatgaaaaca
    12361 gaccaatttc agctcaaccc caccgtaaaa ttacccattg gggtacgaca ttgaattcca
    12421 aggtgagtga cgatgatgtc ttgtaatctg atggtacaat gacagttagt ttgtctagtt
    12481 tataagaaag tactacctga gcttgaatag aactcaggta gctctctgtg aaagaacaaa
    12541 attaatactc aatgaaaatc aaagagcaaa ctaggaaact agccgcaggt tgctcaaagc
    12601 actgctttga ggttgtagat aagactgacg aagtcagtca catatataat ccaaggcgac
    12661 gttgacgtgg tttgaagaga ttttcgaaga gtataaacag aaaggtagag cgcgtgttct
    12721 aatttgaaca cgagtagaaa acttttctaa aaacaaaaac gaaaggatgg gtaaactgta
    12781 ttcgctgaac tgaatacggg cgactctcct ctaaatcaaa attaagaaag gaattgaccc
    12841 caccctaaaa gtagtgggaa aaagatagtt ggtctagcga gcatcgctca ctgcgcccaa
    12901 ctcctatttt cccttcgctt tttgatgggt ttggtatctt tctcaatata aaataaagaa
    12961 aggtagagcg tgtgttttga tttgaacacg agcggaaaac tcggaaaata gataatctga
    13021 ctgaaaaatc aggatttctc gtcaggttcc taattttcag tcgttttctt ctcgctcttt
    13081 gtatcataaa ttatgtctat ccatattgct gctcagcagg gtgaaattgc tgataaaatt
    13141 cttcttcctg gggatcctct tcgtgctaag tttattgcgg agaatttcct tgatgatgct
    13201 gtttgtttta acgaagtgcg taacatgttt ggttacactg gtacttacaa gggtcactgt
    13261 gtatctgtca tgggaactgg gatgggaatg ccatctattt cgatttatgc gcgtgagtta
    13321 atcgtagact acggtgtgaa gaaattgatt cgtgtgggaa ctgcaggttc tttgaatgaa
    13381 gaggttcatg ttcgtgaatt agttttggcg caggcggctg caaccaactc aaacatcgtt
    13441 cgtaatgact ggccacagta cgattttcca caaattgcta gctttgattt gcttgataaa
    13501 gcctaccata tcgccaaaaa acttggtatg actactcacg ttgggaacgt tttgtcatct
    13561 gatgtctttt actcaaatta ctttgaaaag aatatcgagc ttggtaaatg gggagtcaag
    13621 gctgtggaaa tggaagcagc agctctttac tatcttgctg cccaatacca tgttgatgcg
    13681 ctagctatca tgaccatctc tgatagcttg gtcaatccag acgaagacac aactgcagaa
    13741 gaacgtcaaa ataccttcac tgatatgatg aaggttggtt tggaaacctt gattgcagaa
    13801 taattatagc caaaaaggaa gccctttatg aattgtagga cttccttttc ttatccagaa
    13861 attgatctag ctctctctga tttcgaagaa tagtgacttt atgtgaatat tcttggcaaa
    13921 gtttttggta attttctttt tgagttttgc tacgcccatc ccaaagaatc catctgataa
    13981 actcccactc aaagcgttca gggcaatctg ccgccatact ttctctgact tttccacggt
    14041 atttaagata acgcttaaag gctctaaaga gacaggtcaa tggcgaaaaa ttgagaaaga
    14101 tgatttggtc agcttcttgc attcgttctt ggtagtagca ccaagaataa ttaccatcga
    14161 tgacccaagc tttatgcttg gtgagaaagt tttttatctc ggttaacatc cattcgcagt
    14221 cactgtcttg ccaaccaggt tgaaattgga gtgtgtccat gtgcagtttt ggaatggagt
    14281 agtagttaga taacttttct gctatagttg acttaccaga accagaatat ccgataattg
    14341 cgattttcat tttctacttt ttcctatttg gagacaaaaa aacagcctct atggactgtt
    14401 tcttatttag caagtttagc tgaaagacga gctttatcgc ggcttgcttt gtttttgtga
    14461 atcaaacctt tagtttctgc tttatcgata gctgagctag cagcacggaa aagttcttca
    14521 gatgggtttg cttcgaaagc ttttatagca gtacgcatag ctgatttttg agctgagttc
    14581 ttttcgtttt gtttaacgtt caattcagcg cgtttgatag ctgatttaat gtttgccaat
    14641 gttcttacct ccatatttac taactatacc attatatatg aaaacttacg ttttgacaag
    14701 ggaaaattac ttttttaagt aaatttcatc gatttcatgg ttcttgcttt tatgaagaat
    14761 cacttctgca cgatttctgg ttggttcaat ataattttgc agatttgtga gattgatact
    14821 gatccagacc tgatgggcaa aggactccac ttccccaatc ggcatctgag taaaacgata
    14881 atagtagcta tcagggtcgt tttgggctag gctcagcatt ttcaagaaac ggtccagata
    14941 ccaactttca atatcatcca ctccagcatc tacatagatg gaaaagtcaa agaagtcagt
    15001 gatatagaga cgatcgtttt gtggattttg aaagacatta attccctcaa caattacaaa
    15061 atcagcagct ttgacacttt gtttcttttc gggtacgatg tcgtaaactt catgagaata
    15121 gacaggaata tctacatctt gtccattttt gatgcggtcc aagaagttga gaagagcttc
    15181 catatcatag ctttcaggaa atcctttacg atttaaaatc ccctgctcaa tcaaggtttg
    15241 attgggatag agaaaaccat cagttgtaac caactcaacc gtagcatctg taaacgtacg
    15301 ggacagtagg atttgaagta ggcgactggt tgtggatttt ccaacggcaa cactcccaga
    15361 aaccccaata ataaaaggtt gagatttact ttcacgttgg aggaaaattc cttttgaaaa
    15421 ggctaaatct tccttagttc gcttgtaaat ctgaatcaga tgagccaagg ggagatagat
    15481 atctgtaacg tcttggagac tgatttggtc attaaaactc ttgatagatt ccaattcttc
    15541 ttctgtcaaa ggaggtgttg tctttcgatg taaagattgc caagtctggc ggctgatttt
    15601 ttcaaaatgt aaaaattcgt tggtcatttg ttttccccta gatattttgt ctatcatacc
    15661 ataaaaagct aaaaaaataa agcggtttct tgaatgtggt aagtcaatgg tatatagtat
    15721 aagtgatcaa tttattgatg aaataggaga tagattctac tgatttataa gcttataaag
    15781 ggggcttttt agttcacaac ttataggagg tgtactatga aaatcttaaa acgttacata
    15841 ttggaactct gttttatttt aagttttgct ttacctttta taaaaggaac caatgcagat
    15901 aatggtagat gctttgtgga aacctattac ggttttactt ttttgatgga acatgctatt
    15961 gtaacagctg tctttatcta ttcgttttta attgctttct tactaaaaaa acgatgggcg
    16021 aaatggattg ctgctggtag ttattgcttt ttagtcctat ggattgcgac agagggttat
    16081 ttcttccgca tgtcactaga agatttgata cgactttgga caagtttgga aatcctgaca
    16141 caaacgtatc agttgggctt ttatctaaac attttgttag gaattttgtt gataataaag
    16201 tattttaagg ttaagcaata gaaataataa tctgcctgat tatagatgat aaactttttg
    16261 tttttatctg gactgtgata tttatagttc taaatggttt attatttata agtgttgaaa
    16321 gagattgaag aaaatggctt cgtgacttgc tttcttcggt ctttttcgtt ttattatcaa
    16381 tttgtaaacg atttacaatt tgagtcaaat tatggtaaaa tagtgtcatg agtaaaatgt
    16441 attatgcaga aaatcctgac gctgctcacg acattcatga gttgagagtg gacttgttgg
    16501 gacaaaaaat gacttttttg acagatgcgg gtgtttttag caagaaaatg gttgactttg
    16561 gaagtcaact cttgctcaag tgtctggatg tcaaccaagg agagacagtc ctagatgtag
    16621 gctgtggtta tggtccatta ggtttgtcct tggtcaaggt ttacggagtt caggcgacca
    16681 tggttgatat taacactcgt gccttggatt tagctcggag aaatgctgaa aaaaataatg
    16741 caaaagcgac gattttccag tccaacatct atgaacaagt tgaaggacat tttgatcatg
    16801 tcatttccaa tcctcctatc cgtgcgggca agcaagtcgt tcatgaaatc attgagaaaa
    16861 gtaaagattt cttggaaact ggtggggatt tgacaatcgt catccagaaa aaacaagggg
    16921 ctccaagtgc taagtccaag atggaggatg tgtttggtaa ttgtgaaatc cttaaaaaag
    16981 acaaaggata ctatatcctt agaagtgtga aagaatgaga gcagttgatt taatacaaaa
    17041 aaaacgagac ggtcaagaac tgacttcaag tgaaattgaa tggctggtag aaggctatgt
    17101 gtcaggaact gttcctgatt atcagatgtc tgcctttgct atggctgttt attttaaagg
    17161 aatgacgact cgtgaaattt ctgatttaac gatgaatatg gttaagactg ggcaagagtt
    17221 tgacctgtca gccattgatg gtgtgaaagt tgacaagcat tctactggtg gtgtcggcga
    17281 taaggtaacc ttgattttag ctcctcttgt tgctagcttc ggtgtgcctg tagcaaaaat
    17341 gagtggtcgt ggtcttggcc atacaggcgg aacaattgat aaattggagt ccattagggg
    17401 ctatcaagta gaacgtagtc aagaggattt tattcgtcag gttcaggata ttggagtatc
    17461 tgtcattggg caatcagacc agctggtcaa agcagataag cttctctatg cccttcgtga
    17521 tgtgaccgca actgtcgaca cgattccttt gattgcgagt tcggtgatga gcaagaaaat
    17581 tgcggcaggg gcggatgcta ttttgctaga cgtgactgtc ggtgagggtg ccttcatgaa
    17641 gacggttgat gaggcgcgtg agttggctca aactatggta gatcttggta aggttgttgg
    17701 acggaagaca gtagcagtga ttacggatat gagccagcct ttgggacgag cgattggaaa
    17761 tcgtctggaa atacttgaag cattggagat tttgcaaggt caaggacgtc aggatattac
    17821 ccactttatt tgtgaattgg ctcaaattat gcttggtttg gcaaatgtta acaagactgt
    17881 tgaagaagtt caccaacatc ttgagaatgg tcaagcactg gctaagtttg aggaaatggt
    17941 gcaagcccaa ggtggagact tggaagacct ctatcgtcct gtaaatgtag ctcatgtggt
    18001 ggaaatccct gctcaggaaa cgggtgtcat ttcagctctt ccagctatgg actttggtct
    18061 ttatgccatg aggctggggg ctggtcgtgc agttaagtct gatgccttgg actatgaaac
    18121 aggaattgtt tttgaaaaga agattggaga ctccgttcaa aagggtgaaa ttgttgcaaa
    18181 agtatacaca aatgaaaaaa ttccacctca actagttaca gatttccaaa aatgtgttaa
    18241 aataagtgat gaagtgaaaa aaatccgtga aattgtagaa atcatctcct aaatggaggg
    18301 aaaaccgaaa tgaaattaaa taaatatata gatcatacgc ttttaaaaca agatgcaaag
    18361 aaaaaacaaa ttgatagttt gttgtctgag gctagagagt atgactttgc cagtgtttgc
    18421 gttaatccga cctgggttga acatgctaaa aaaggacttg aaggcacaga tgttaaggtt
    18481 tgcacggtag taggtttccc tttgggagca acaacttcag ccgtgaaagc atttgagaca
    18541 aaagaagcta tccaaaatgg tgcagatgag attgatatgg tgatcaatgt tggagctctc
    18601 aaatcaggta atttagcctt ggttgagtca gatattcgcg cagtagtgga agcaagtggt
    18661 gataagttag tgaaagtcat tattgaagct tgccttctga cagaccaaga aaaaattgtt
    18721 gtttgccaat tggcccaaaa agctggggct gactttgtta aaacatctac tggtttttca
    18781 actggtggtg ctacgatagc agatgttaca ttaatgcgtg aaacagttgg atctgatatg
    18841 ggagtcaagg ctgctggtgg agctcgttct tatgcagatg ctcttgcctt tgtcgaagca
    18901 ggtgcgaccc gtatcggaac gtcagctggg gtagctattt taaaaggaga attggcagat
    18961 ggcgactact gagttgattg aactggcaat tgaaaccagc aaacatgcct atgtccccta
    19021 ttctcacttt cctatcggag cagttttagt agccaaagac ggaagtgttt acacgggagt
    19081 gaatatcgaa aatgctagct atcctttgac caattgcggt gagagaacag ctatttttaa
    19141 ggctatatct gaaggccaaa gagaattttc agaattgatt gtctatggtc aaactgaaaa
    19201 accgatctcg ccatgtggtg cttgtcgcca agtgatggct gaattttttg aacaagatct
    19261 aaaagtgacc ttagtcgcaa aagataaatc gacggtcgag atgacggtcg gggaattact
    19321 tccatactct tttacagact taaactagtc tgagtcgctc tctgagtggc acggtccttg
    19381 tggccaacca atccatactt gcaacatcgt tgcacatctt atttaggagg ttcagtaatg
    19441 aacaagaaac aatggctagg tcttggccta gttgcagtgg cagcagttgg acttgctgca
    19501 tgtggtaacc gctcttctcg taacgcagct tcatcttctg atgtgaagac aaaagcagca
    19561 atcgtcactg atactggtgg tgttgatgac aaatcattca accaatcagc ttgggaaggt
    19621 ttgcaggctt ggggtaaaga acacaatctt tcaaaagata acggtttcac ttacttccaa
    19681 tcaacaagtg aagctgacta cgctaacaac ttgcaacaag cggctggaag ttacaaccta
    19741 atcttcggtg ttggttttgc ccttaataat gcagttaaag atgcagcaaa agaacacact
    19801 gacttgaact atgtcttgat tgatgatgtg attaaagacc aaaagaatgt tgcgagcgta
    19861 actttcgctg ataatgagtc aggttacctt gcaggtgtgg ctgcagcaaa aacaactaag
    19921 acaaaacaag ttggttttgt aggtggtatc gaatctgaag ttatctctcg ttttgaagca
    19981 ggattcaagg ctggtgttgc gtcagtagac ccatctatca aagtccaagt tgactacgct
    20041 ggttcatttg gtgatgcggc taaaggtaaa acaattgcag ccgcacaata cgcagccggt
    20101 gcagatattg tttaccaagt agctggtggt acaggtgcag gtgtctttgc agaggcaaaa
    20161 tctctcaacg aaagccgtcc tgaaaatgaa aaagtttggg ttatcggtgt tgatcgtgac
    20221 caagaagcag aaggtaaata cacttctaaa gatggcaaag aatcaaactt tgttcttgta
    20281 tctactttga aacaagttgg tacaactgta aaagatattt ctaacaaggc agaaaaagga
    20341 gaattccctg gcggtcaagt gatcgtttac tcattgaagg ataaaggggt tgacttggca
    20401 gtaacaaacc tttcagaaga aggtaaaaaa gctgtcgaag atgcaaaagc taaaatcctt
    20461 gatggaagcg taaaagttcc tgaaaaataa tggatggaag tcatttctta ggaagcggcc
    20521 aagggccgct tttttaagag ttgagacaaa gtcattttgt ctcagtaaag cttgctacta
    20581 gacaaactag caagttttat tgaaataaaa taagactctg aaaggaagag cacatggcac
    20641 acgaaaatgt cattgagatg cgtgatatta ccaaggtgtt tggtggattt gttgccaacg
    20701 acaaaatcaa cttgcaccta cgaaaaggtg aaattcatgc acttttagga gaaaatgggg
    20761 ctggtaagtc cacgctaatg aacatgttag caggccttct tgaaccaact agtggtgaaa
    20821 tcgcggtcaa cggtcaagtt gtcaatctcg actccccatc taaagcagct agcttgggaa
    20881 tcgggatggt tcaccagcac tttatgttgg ttgaagcctt cacagtggct gaaaacatca
    20941 ttttaggtag tgaattgact aaaaatggtg tgctagatat cgctggagct agcaaagaaa
    21001 tcaaggctct ttctgaacgt tatggcttag ctgttgaccc ttctgccaag gtagcagata
    21061 tctcagttgg agcccaacaa cgtgtagaaa ttttaaaaac actttatcgg ggggctgata
    21121 tccttatctt tgacgaacca acggctgttt tgactccatc agaaattgat gagttgatgg
    21181 ctattatgaa aaatcttgtc aaagaaggaa aatcaattat cttgattacc cacaaattgg
    21241 atgaaattcg cgcagtttct gaccgtgtta cagttatccg tcgtgggaaa tcaattgaaa
    21301 ccgttgaaat tgcaggggct accaatgctg atttggcgga aatgatggta ggacgttctg
    21361 tttcctttaa aacagagaag caagcctcta aaccaaaaga agtggttttg tctatcaaag
    21421 atttggtggt caatgaaaac cgtggtgttc cagctgttaa aaatctgtcc ttggatgttc
    21481 gtgctggaga gattgttggt attgcgggga ttgatggaaa tggtcagtct gaactgattc
    21541 aagccattac aggtcttcgt aaggttgaat ctggtagcat tgagctaaaa ggagattcaa
    21601 ttgtaggctt gcacccacgt cagattacag aactaagtgt tgggcacgtt ccagaagacc
    21661 gtcaccgtga tggcttgatt ttggaaatga tgatatctga aaatattgcc cttcaaacct
    21721 actataaaga accacatagt aaaaatggaa ttttgaatta ttcaaatatt acttcttatg
    21781 ctaaaaagct gatggaagag tttgatgttc gcgctgccag tgaattagtt cctgcagctg
    21841 cactctcagg aggaaatcaa caaaaagcaa ttattgctcg tgaaattgat cgagatcctg
    21901 atctccttat cgttagccag ccaactcgtg ggttggatgt cggtgccatt gagtatatcc
    21961 acaaacgctt gattgaagag cgtgataatg gcaaggctgt ccttgttgtc agctttgaat
    22021 tggatgagat tttaaacgtc tcagaccgta ttgccgttat ccacgatggt aagattcaag
    22081 gtattgtatc accagaaaca accaataaac aagaacttgg tgtcttgatg gctggtggaa
    22141 acttgggaaa ggagaagagt gatgtctaaa aaattacaac aaatttcggt tcccttgatt
    22201 tctgtatttc taggaatttt actcggagcc attgtcatgt ggatcttcgg ttatgatgct
    22261 atttggggct acgaagaatt gttctataca gcctttggca gtctgcgtgg gattggagaa
    22321 atcttccgtg ctatgggtcc tctggtcttg attggtcttg gttttgcggt tgccagtcga
    22381 gctggtttct ttaacgtcgg acttcctggt caggctttgg caggttggat tctcagtggt
    22441 tggtttgcgc tgtcgcatcc agatatgccc cgtcccttga tgattctagc aaccatcgtg
    22501 attgccttga ttgctggtgg gattgtcgga gcgattccag gtattcttag ggcctatcta
    22561 gggacgtcag aggttattgt aaccatcatg atgaactaca ttgtcttgta tgtagggaat
    22621 gcctttatcc atgctttccc taaagacttc atgcaaagta cagattcgac cattcgtgtt
    22681 ggggctaatg caacctatca gacaccttgg ttggctgagt tgactggtaa ctcacggatg
    22741 aatattggta ttttctttgc catcattgcc gttgcagtta tttggttcat gctcaagaaa
    22801 acaactcttg gttttgaaat ccgtgcagtt ggtcttaatc cacatgcttc agaatatgct
    22861 ggtatttctg ccaagcggac tattatccta tctatgatta tttcaggtgc cttggcaggt
    22921 cttggtggag ctgttgaagg tttgggaacc ttccagaacg tctatgttca aggttcgtca
    22981 ttagctatcg gatttaacgg aatggcggtt agtttgcttg cggccaactc accaattggt
    23041 atactctttg cagccttcct atttggcgtt ctccaagttg gggctcctgg tatgaatgcg
    23101 gcgcaggtac catctgagct tgtcagcatt gtaacagcgt ctattatctt ctttgtcagt
    23161 gttcattacc ttatcgaacg ctttgtcaaa ccgaaaaaac aagttaaagg aggtaagtaa
    23221 agatgtctat tataaccttg ctccccctct tggtgtcttc tatgctgatt tactcagcac
    23281 ctctcatctt tacaagtatc ggtggtgttt tctctgaacg tggtggtgtg gtaaacgttg
    23341 gccttgaagg aattatggtt atgggtgcct tttctggagt tgtctttaac cttgaatttg
    23401 cagaacaatt tggagcagca actccatggc tatccttgct tgtagcagga ttggttggta
    23461 gtgttttctc tatcatccac gcagcagcga cggttcattt ccgtgcagac catgttgtca
    23521 gcggtacggt attgaacttg atggcgcctg ccttggctgt tttcttagtt aaagttcttt
    23581 ataacaaagg acaaaccgac aacctaagtc aaacttttgg acgctttgat ttcccagtct
    23641 tggcaaatat cccagtgatt ggtgatatct tcttcaagtc aactagtcta cttggttatc
    23701 tggcgattgc cttctcattc cttgcttggt ttattctctt taagactcaa tttggtcttc
    23761 gtctccgctc tgtcggtgaa catcctcaag cagcggatac cttgggaatc aacgtctaca
    23821 agatgagata tttagggatt attatttcag gttttctagg tggaattggc ggagcgattt
    23881 atgcgcaatc aatctcagtt aacttctcag tgacaactat tgttggacct ggatttatcg
    23941 cccttgctgc gatgatcttt gggaaatgga atccaatcgg tgctatgctt tctagtctct
    24001 tctttggact ttcacaaagt ttggctgtta tcggttctca attgccgttc ctacaaggag
    24061 tgccagcggt ttatcttcaa attgcacctt atgttttgac aattcttgtc ttagcagcct
    24121 tctttggaaa agcagtcgca ccaaaagcag atggtatcaa ctacatcaaa tcaaaataag
    24181 catacaaaaa aacgtcagtc caatgactga actgcccccc aaaagttaga cagaaaaaat
    24241 ctaacttttg gggtg
//
LOCUS       DAVBBS010000030        23487 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVBBS010000030 DAVBBS010000000
VERSION     DAVBBS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23487
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.030"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            298..558
                     /locus_tag="VQU32_001177"
     CDS             298..558
                     /locus_tag="VQU32_001177"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2472639.1"
                     /translation="MSGGETKRVALAKIILKNPPLIFADELTASLDSTTSQGIMKLLL
                     GMKNKNRLIIIATHNPDIWNQADEVINLNQLLQSIYIFYILK"
     gene            complement(662..1132)
                     /locus_tag="VQU32_001178"
     CDS             complement(662..1132)
                     /locus_tag="VQU32_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2472640.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQTLLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(1149..3422)
                     /locus_tag="VQU32_001179"
     CDS             complement(1149..3422)
                     /locus_tag="VQU32_001179"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HET2472641.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLASDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            3769..6870
                     /locus_tag="VQU32_001180"
     CDS             3769..6870
                     /locus_tag="VQU32_001180"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET2472642.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQGVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRLAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEVREGAFYYIKGKVQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSDELEAKLNEIVMKT
                     IYR"
     gene            6952..7959
                     /gene="pfkA"
                     /locus_tag="VQU32_001181"
     CDS             6952..7959
                     /gene="pfkA"
                     /locus_tag="VQU32_001181"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET2472643.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            8016..9521
                     /gene="pyk"
                     /locus_tag="VQU32_001182"
     CDS             8016..9521
                     /gene="pyk"
                     /locus_tag="VQU32_001182"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET2472644.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(10253..10478)
                     /locus_tag="VQU32_001183"
                     /pseudo
     CDS             complement(10253..10478)
                     /locus_tag="VQU32_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            11326..12198
                     /locus_tag="VQU32_001184"
     CDS             11326..12198
                     /locus_tag="VQU32_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HET2472645.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(12344..12688)
                     /locus_tag="VQU32_001185"
                     /pseudo
     CDS             complement(12344..12688)
                     /locus_tag="VQU32_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            12831..13121
                     /locus_tag="VQU32_001186"
     CDS             12831..13121
                     /locus_tag="VQU32_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472646.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEYPELYAVAQTSGEIPEKNAKNCVRLSQLSLNKR"
     gene            13145..13861
                     /locus_tag="VQU32_001187"
     CDS             13145..13861
                     /locus_tag="VQU32_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2472647.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSIGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIISDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(13990..14805)
                     /locus_tag="VQU32_001188"
     CDS             complement(13990..14805)
                     /locus_tag="VQU32_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HET2472648.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(14802..15707)
                     /locus_tag="VQU32_001189"
     CDS             complement(14802..15707)
                     /locus_tag="VQU32_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HET2472649.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGMLFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            16105..18234
                     /locus_tag="VQU32_001190"
     CDS             16105..18234
                     /locus_tag="VQU32_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HET2472650.1"
                     /translation="MDKKYEKISQDLGVMLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRAQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYTAVKELFKRL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVSVGD
                     LVTVWVKKIDTEREKVNLSLLAPNETD"
     gene            18221..18670
                     /locus_tag="VQU32_001191"
     CDS             18221..18670
                     /locus_tag="VQU32_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HET2472651.1"
                     /translation="MKLTDYVKQVSLEDFGRAFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQKLGMEVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPLKLYRCQSCQQRYQRKRRIDTKRYRCGLCRGKLLLINQPED"
     gene            18729..19001
                     /locus_tag="VQU32_001192"
     CDS             18729..19001
                     /locus_tag="VQU32_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET2472652.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(19355..19546)
                     /locus_tag="VQU32_001193"
     CDS             complement(19355..19546)
                     /locus_tag="VQU32_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472653.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            19974..20732
                     /locus_tag="VQU32_001194"
     CDS             19974..20732
                     /locus_tag="VQU32_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2472654.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            20734..22722
                     /locus_tag="VQU32_001195"
     CDS             20734..22722
                     /locus_tag="VQU32_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2472655.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNEGVKEQKALTLNDHQFSVKEEFTKDFIVNHVPNQF
                     NILTADYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVNASEAEQLKVAEEYEKYLQKF
                     NAQLNTEGNYVYGSTLADASAQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFLHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(22764..23408)
                     /locus_tag="VQU32_001196"
     CDS             complement(22764..23408)
                     /locus_tag="VQU32_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HET2472656.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATTNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNEEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
BASE COUNT         7208 a         4233 c         4959 g         7087 t
ORIGIN      
        1 ttgttttgtc gcttttcatt ataggtcata tgggactttt tttctacaat aaaataggct
       61 ccataatatc tatagtggat ttacccacta caaatattat agaacccttt tttattgaca
      121 gtatcaggat atctttttca aatttttggt ttgttagata atgaaaatgt ttctatcaat
      181 ctagatttag gattaataaa tcgtaaatgt aattataaag aaaaagataa gttaaaaaaa
      241 gatgtattag caagagttgg tctaactcat attcatctat aacaaaagat atatgaattg
      301 tctggcggtg aaacaaaacg tgttgcttta gcgaaaatca ttcttaaaaa tccaccatta
      361 atattcgcgg atgagttgac tgcatcactc gattcgacga cttcacaagg aattatgaaa
      421 ttgttattag gtatgaaaaa taaaaatcgt ctaatcatca ttgcaactca taatcccgat
      481 atttggaatc aagcagatga agttataaat ttaaatcaat tattacagtc aatatatatc
      541 ttctatattt taaagtaatt ttatctgaat ccgttaagag actattgtcc caggctcttt
      601 ctgatgttaa agactttcta ccaggttttt taaaagcata attgttagtt gtagtcattt
      661 attattcttc aaagaaaaat ggtggggcga attttttcag ttcttcaaag cacttttgag
      721 cagtatctgc atcttcacag atgataagac agacatcatt accacaaagg gtagcgatag
      781 cgtcagggaa gctcaaagta tcaatgatag aaccaaagga ttgagccagt ccaggaaggg
      841 tttttagtag gacttggtgt tgaactgggc gcatccagac aagggcgtct tccatgtaga
      901 gttcgagacg tttttcccat tttgagatgg aaccattgtt aagaacataa taagcgctat
      961 cttcttcgcg gacttttgat aggttcatat ttttgatgtc gcgtgagagg gttgcctgag
     1021 ttacttgaat gtcgttctca gcaagaaggg tttgcaactc agcctgtgta tgaatcttgt
     1081 tttttgtgat aagagcgcgt ataagttggt ggcggtgttc tgatttattc ataataatgt
     1141 aactcctttt agcaaggtaa ggtaagcatg gactgagcga ggtcgacagt caagtggtag
     1201 tctgtattgt cacggatggt gatttcaaag tcagtagtat agaggactaa acggagagtg
     1261 tctccttctt ttagcttgta aatagttggc tgcagttcaa attgaacgtc catccattca
     1321 tctgcagtaa tatcctctac taacagtaaa tcatttctat tttgtaaatt aaggtaacct
     1381 tttgtcacga ctctttgtgc ctctggtcta aatggcaatt cacagagatt ttccaacatg
     1441 tgatagcgac cgttgtcaat ggttctagca cttaaaatag ctggataagg ttgtaggtat
     1501 ttcttttgcc caaattctag cagttgggca gataagagcc ccttgtttgt actggatttg
     1561 atacgaagat tgagctgagc gcgaccgttt aggtggagat ctttagtcac aggaaggtta
     1621 atagtaatct gattagcttt cccttgatag agctctgtat tgaaggtttg gtatgtctta
     1681 ccatagcgct caaaatcctt atctgggtac tggttttgaa tagcttgctc ttcttgacca
     1741 agtgagaagg tttcacagtt ttcttgccca ccgaagttat caagtgataa ccaagtctgt
     1801 ggagctgtat tgtcctgcca gataacagta ggaagttgaa agtctgtttc ctgtcctagt
     1861 aatttcttgg tcaataaggc atttatggac tcacggaagt caattgattg ccaattgttc
     1921 atgtaaacat gggcaccatt atggaaaaag agatgcttgt gtatatgagt aggaagagca
     1981 tggaacatct ggtaaacatg aagtggtttg acattccaat cctgagaacc atgagtaaag
     2041 acaacctctg cctttacttt atgggcattg agcagataat tgcggtcatg ccaaaactga
     2101 ttgtagtccc cagtttttcg gtctagttga gctttcactt tttctaagtc agcttggtga
     2161 gcttcattgc cacggatata gtcgctagcc aagagattac gagaataggt taactcagca
     2221 agggagtcaa agtcctcacc tggataacca cctgggctag tcaccagacc gttttcacgg
     2281 tagtagttgt accatgatga aattcctgcc tcggcaatga taacttctaa accatcgact
     2341 cctgtagtcg caagaccatt ggacatggta cctagatagg aaagtcccgt tgtagcaact
     2401 tttccgtttg accaatcagc cttgacttga cgctggcgcg tgtgatcagt aaaggcacgg
     2461 caacggccgt taagccaatc gatgacattt ttataagcct cgatttgctg gtagtctcca
     2521 ttagtcatga aacctgtcga gtctttggta ccaacacctg agacatagag attggcaaag
     2581 cctctcggaa ggaagtagtc gtttagtgta tagctagagt tgatgtgagt tagcttttcc
     2641 tcagcctctg ctataagctc agctttacct tggggttgga cgagatttag ttgaggtttc
     2701 tctagctcaa tcttgtgagg aagtttaacc tcaagctcgc cctccatctt gtagagagcc
     2761 ttgtcactag ccttgtcatt ggttccctga tgataagggc tggctgtcat gatggcaggg
     2821 atttttccat caaaacgagg gcgaataatg ctaaccttta ctaggtctga tagccctttt
     2881 tggtcagtat cgacacgaga ctcaacgtaa acgacttcac gaatgacatc ctggttagaa
     2941 aaagtagcca aactcttgcc gttaaagtag tggtagtcat tatcctccgg aataagacca
     3001 tcactaacaa gttggtcgat aagagtattt ccttttttgg tgcgagtatt gagtaactga
     3061 tagagatttt caatcaagtc accatatata atgggaaatc cagtttcttt acgaaaaacg
     3121 tcactatctt cgaagtcaac caaataagaa aagcctaaaa gttgaaaagc aacagtataa
     3181 aaaatatctg ctgtcagttc atcttctgat tgaaaaaatg tcagcaggtc tgttttttta
     3241 tcagctgcta ggatagaaag tgggtagttg gtgtcttgat aagtgaaaaa gaaacgacgt
     3301 aaaaaggttt caagtgagtc tttgtgattg gctgtatttt gtaaatcaaa gccacatttt
     3361 tttagttcag ataagacatt ttcttttgga aaattgatat aactatattg attaaaacgc
     3421 atagaacctc catatagaat gacagttaag gttattatat caaaaaaaaa gcagaaaggg
     3481 aattgttaac ttcaaaagga aataatccaa taaaaatgaa taaagtacta aattcaatat
     3541 agagaacaga gtaacaataa gaataaatag atagggtata aaagttctag gagatttata
     3601 ttatatgctt tctgttttta tatacaatat agtataaata taaaaatgat gacaaaaata
     3661 caaatgaata gaaaataaat tagtaagctg atgaaatttt tctcaagaga agccatttat
     3721 aggtgaaaat ggtataatat agtgagaagg atagaggaga agtgtaaatt gatcgcacaa
     3781 ctagatacaa aaacagtcta tagttttatg gaaagcgtca tttcgatcga aaagtatgtg
     3841 agagcagcta aagaatacgg ctacactcat ttggctatga tggatattga caatctttat
     3901 ggcgctttcg actttctaga gattacaaaa aaatacggca ttcatccttt gctagggctt
     3961 gaaatgacag tgtttgtaga tgatcaggga gtgaatttgc gctttttagc tctatctagt
     4021 gtgggctatc agcagttgat gaagctttcg acagccaaga tgcaggggga gaaaacttgg
     4081 tcagtcctgt cccagtacct ggaggatatc gcggtcattg tgccttattt tgatagagtt
     4141 gagtcgttag aactaggctg tgattactat ataggggttt atccagaaac actagcaagc
     4201 gaatttcatc atcctatctt acctctttat cgggtcaacg cttttgaaag cagggataga
     4261 gaagttcttc aagttttaac agcgattaaa gaaaatctac cgctcagaga agttcccttg
     4321 cgttcgagac aagatgtctt tatatcagca agttctttag agaaactatt ccaagagcgt
     4381 tttccgcaag ctttggacaa tttagaaaag cttatttcag gcatttctta cgacttggat
     4441 actagtctga aactgcctcg ttttaatcca gctagaccag cagtagagga gttgagagag
     4501 cgtgctgaac tggggcttgt tcagaagggg ttgactagta aagaatatca agatagacta
     4561 gaccaagaat tgtctgttat tcatgatatg ggctttgatg attatttctt ggttgtttgg
     4621 gatttgttgc gttttggacg atcgaatggc tattatatgg gaatgggaag gggttctgca
     4681 gtaggcagtt tggtttctta tgccttagac atcacgggga ttgacccagt agagaaaaat
     4741 ctgatttttg aacgctttct taatcgtgaa cgctatacca tgcctgatat tgatattgat
     4801 atcccagata tttatcgtcc agattttatc agatatgttg gtaataaata tggtagtaaa
     4861 catgcggcac aaatcgttac tttttcaacc tttggagcca agcaagctct tcgagatgtc
     4921 ttgaaacgct ttggtgtgcc agagtatgaa ttatctgcaa ttactaagaa aatcagtttt
     4981 cgtgacaatc ttaagtcggc ctatgagggc aatctccagt ttcgtcagca aatcaatagt
     5041 aagttagaat accaaaaagc ttttgagatt gcttgcaaga tagagggcta tccaaggcaa
     5101 acctctgtcc atgcggctgg tgttgtaatt agtgaccaag atttaaccaa ctacattcct
     5161 ctaaagtatg gtgatgaaat tccactgact cagtatgatg ctcatggagt tgaggctagc
     5221 ggacttttga agatggactt tctgggacta cgaaatttga cctttgtcca gaagatgcaa
     5281 gagttgcttg ctgaaacaga aggtattcat ctgaaaattg aagaaatcga tttagaagac
     5341 aaagaaacat tagctttatt tgcctctggt aatacaaaag gtatctttca atttgagcaa
     5401 ccaggtgcca ttcgtctgct taagcgtgtg caaccagtct gttttgaaga tgtcgtcgcg
     5461 actacttctc taaatcgacc gggtgctagt gactatatca ataattttgt ggcaagaaag
     5521 catgggcagg aagaagtgac tgttctggat ccagtactgg aggatatttt ggctccaacc
     5581 tacggcataa tgctctatca ggagcaggtt atgcaggttg cccagcgact tgccggattt
     5641 agtcttggga aagccgatat tttgcgtcgg gctatgggga aaaaggatgc ctctgccatg
     5701 catgagatga gggcttcctt tattcaaggt tcattagaag ctggtcatac tgtggaaaaa
     5761 gcagagcagg tctttgatgt tatggagaag tttgcaggtt atggttttaa caggtcacac
     5821 gcctatgcct actcagcctt ggccttccag ttggcttatt tcaaaacgca ttatccagcc
     5881 attttttatc aggtcatgtt aaattcttcc aacagtgatt acttaataga tgcacttgaa
     5941 gcaggttttg aagtagcctc tctatccatc aacaccattc cctatcacga taaaattgcc
     6001 aacaaggcca tctatctagg tttgaaatcc attaaaggag tcagtaatga tttagctctc
     6061 tggattattg aaaatagacc ttattctaac attgaagatt ttatagctaa attacctgag
     6121 aattatctga aacttcctct gctagaacct ttggtaaaag ttggtctttt cgattcattt
     6181 gaaaaaaatc gtcaaaaagt atttaataac ttagctaatc tatttgaatt tgtgaaagag
     6241 ttgggaagtt tgtttggaga tgctatttat agttggcagg aatcggaaga ttggacggaa
     6301 caagaaaaat tttatatgga acaagagctt ttagggatag gtgtcagcaa acatccacta
     6361 caagctattg caagtaaggc tatttacccg attaccccaa tcggaaattt gtcagaaaat
     6421 agctatgcta ttatcttggt tgaagttcag aaaataaaag tgattcgtac caaaaagggt
     6481 gaaaatatgg ccttcttaca ggcagatgat agtaagaaaa aattggatgt tacactcttc
     6541 tcagatttat atcgacaggt tggtcaggaa gtaagagaag gagcattcta ctatatcaaa
     6601 gggaaagtcc aatcacgtga cggtcgtctg caaatgattg cacaagaaat aagagaagca
     6661 gttgctgaac gcttttggat acaggtgaaa aatcatgaat cggatcaaga aatttcacgt
     6721 attttagaac aatttaaagg cccaatccca gtcatcatcc ggtatgaaga ggaacagaaa
     6781 accatcgttt ctccccatca ttttgtagct aaatctgatg aattagaggc gaaattgaat
     6841 gaaatcgtta tgaaaacgat ttatcgctaa aaatacggaa aatagaagaa ttttaaaatc
     6901 aaatgtgata taatcaagaa gaatgttaaa agaaaaagga gcataaccaa tatgaaacgt
     6961 attgctgttt tgactagtgg tggagacgcc cctggtatga acgctgccat ccgtgcagtt
     7021 gttcgtcaag caatttcaga aggaatggaa gtgtttggta tctatgacgg atatgctggt
     7081 atggttgccg gtgaaattca tcccctagat gcagcttcag taggggacat catttctcgt
     7141 ggtggtactt tccttcactc agctcgttac ccagagttcg ctcaacttga agggcaactt
     7201 aaagggattg agcaattgaa aaaacacgga attgaaggtg tagttgttat cggtggtgac
     7261 ggatcttacc acggcgctat gcgtttgact gaacatggct tcccagctat tggtcttcca
     7321 ggtacaatcg ataacgatat cgttggtact gactttacaa tcggttttga cacagcggtt
     7381 actactgcca tggacgctat cgataagatt cgtgatacat catcaagtca ccgtcgtact
     7441 tttgtaatcg aagttatggg acgtaacgct ggtgatatcg ctctttgggc tggtattgca
     7501 actggtgctg atgaaatcat catccctgaa gcaggcttca agatggaaga tatcgtagca
     7561 agcatcaaag ctggttatga atgtggtaaa aaacacaata tcatcgtctt agctgaaggt
     7621 gtgatgtcag cggctgaatt tggtcaaaaa cttaaagaag ctggagatac aagcgacctt
     7681 cgtgtaacag aacttggaca tattcaacgt ggtggttctc caactgcgcg tgaccgtgtt
     7741 ttggcgtcac gtatgggtgc acatgctgtt aaacttctta aagaaggtat cggtggtgtt
     7801 gcggttggta ttcgtaacga aaaaatggtt gaaaatccaa ttcttggtac tgcagaagaa
     7861 ggggcattgt ttagccttac tgcagaaggt aagattgtgg ttaacaaccc gcacaaagcc
     7921 gatattgagc tatctagctt gaataagagc ttgtcataat ttacaattta gttaataaga
     7981 ccaaaaaggt catatataaa ggagtcacaa aaatcatgaa caaacgtgta aagatcgttg
     8041 caactttggg tcctgcggta gaaatccgtg gtggtaaaaa attcggtgag gacggatact
     8101 ggggtgaaaa acttgatgtt gaagcttcag ctaaaaacat tgctaaattg attgaagctg
     8161 gtgctaacac attccgattc aacttctcac acggcgacca ccaagaacaa ggtgagcgta
     8221 tggcaactgt taaacttgcg gaaaaaattg caggtaaaaa agttggtttc cttcttgata
     8281 caaaaggacc tgaaatccgt acagaattgt tcgaaggtga agctaaagaa tattcataca
     8341 aaactggtga aaaaattcgt gttgcaacta aacaaggaat caaatcaact cgtgaagtga
     8401 ttgcgttgaa cgttgctggt gctcttgata tctatgatga tgttgaagtt ggtcgtcaag
     8461 ttttggttga cgatggtaaa cttggtcttc gtgtggttgc taaagatgat gcaactcgtg
     8521 aatttgaagt tgaagttgaa aacgatggta tcatcgctaa acaaaaaggt gtgaacatcc
     8581 ctaacactaa aattcctttc ccagctcttg ctgaacgcga taacgacgat atccgtttcg
     8641 gtcttgaaca aggtatcaac ttcatcgcaa tttcattcgt acgtactgca aaagatgtga
     8701 acgaagttcg tgcaatctgt gaagaaactg gaaacggaca tgttcaatta ttcgctaaaa
     8761 tcgaaaacca acaaggtatc gataacttag acgaaatcat cgaagcagct gatggtatta
     8821 tgattgctcg tggtgatatg ggtatcgaag taccgttcga aatggttcca gtttatcaaa
     8881 aaatgattat caagaaagtc aatgctgcag gtaaagttgt tatcactgca acaaacatgc
     8941 ttgaaacaat gactgaaaaa ccacgtgcaa ctcgttcaga agtatcagac gtattcaacg
     9001 ctgttatcga cggaactgac gctacaatgc tgtcaggcga gtctgcaaac ggtaaatacc
     9061 cactcgagtc agtaactaca atggctacaa tcgacaagaa tgctcaagct cttcttaatg
     9121 aatacggacg tcttgattca gattcatttg agcgtaactc taagacagaa gtaatggctt
     9181 ctgctgttaa agatgctact agctcaatgg atatcaaatt ggtcgtaact cttactaaga
     9241 caggtcatac tgcacgtttg atttctaaat accgtccaaa tgctgacatc ttagcattga
     9301 catttgacga attgacagaa cgtggcttga tgttgaactg gggtgttatc ccaatgttga
     9361 cagatgctcc atcttcaact gacgatatgt tcgaaatcgc tgaacgtaaa gcggtagaag
     9421 caggtctcgt tgagtcaggc gatgatatcg ttatcgttgc tggtgtgcca gtaggagaag
     9481 ctgttcgcac aaacacaatg cgtatccgca cagtacgtta agaaaaatat aaaaacctat
     9541 catatccagc tttagagctt gtgtgatagg ctttttgtat agagggtaag aaataggcaa
     9601 aactttcata atggattgat actcttcgaa aatcaaattc aaaccacgtc agcgtcgcct
     9661 taccgtatat atgttactga cttcgtcagt tctatctaca acctcaaagc agtgctttga
     9721 gcaacctgcg gctagcttcc tagtttgctc tttgattttc attgagtatg aaataagata
     9781 tgcacaaatt gattaggaaa ttcaaatgaa tttctacaaa tgttttagca atcgtaatgt
     9841 acttgtctag attcgatctg atatattttc gatttaatga tatggtattt aaaacctcca
     9901 aagtagcttg ctccattctt ttacttacgt gagtgtagat gttatttact gttttagcgt
     9961 ttttgtgttc cactctaacc attatagcat tcttctcagc tagtgtacta aggagtgtgt
    10021 gcctgaaaat atgggaacta aggggctggt ttatcggttt ctctagttta gtatttgcct
    10081 tttgcaaagt gatgttaaat gcctttctct aaatttacat atcactattg tttaacaaaa
    10141 tctaatctac tttaggtcac ttattctttt tttgaaatgt agaatgaact ttttcaaagt
    10201 ttttcgaatc ttttaaaatc tgtttgtttt atatcgccat tctccccctt ttttaattct
    10261 ccctatatag cctgacaact ttcccgatgg tacgaatatg gttgctttcg tctaggtgga
    10321 tgtcggggta tttgggattg agttttttta aggtagcctt ggcggagttt cttgacatag
    10381 ttagcgccgt ctacttggaa gatgccgatg gtattatagt caatctgtgg ggtattcttg
    10441 ataaataggt agtcgctgtt tcttatcttt ggctccatgg acttgctgac gacataagcg
    10501 attgggtcgt agtcgtctgg gataatggaa actccataac taaatcgtcg tcctgcatcg
    10561 agcggctacc tgcagagata aactacctaa cacgagagta agtagtctgt ctgtagtcgt
    10621 ccagtctgat gatttttacg atatttcgtt tttctgctca tacagttgcc tctcggcata
    10681 ggtcagaact ttaccttgtc tgggtggtgc ccgttggtcg tagatagatt ggatatcgct
    10741 aggagaatcc ttttgaactg gaggaaagag ggtatcaatc aagctactga atactttagc
    10801 taagtcaaat atagtatttt tcttagtaga cctaaccctt ttttcataat ttccaatggt
    10861 gtttttactt ataccaatct tagtacccaa ttcttattga gtccaaccat tactagtcta
    10921 tattgtttta tagttgattg agtttggaat agtacgctgt agctgctaaa acatttctag
    10981 gaattaattt gactttccta atagagttgt tcatatctta tttcaatcta ctatgttttt
    11041 cacctctaac aatcgcaatc tcttctttat ccatgaatga aatcgctttc tatttttgta
    11101 agtaaagcat cacacgaaat ccacgaaaat gaaaaccttt gttgtgtttt cgtaaaaaat
    11161 ttgttgacag agcacgaaac gcctactata attaaatcaa gcttaaggaa ataacaaaaa
    11221 aacaaatcgg agggaaagac gatgaacaca ttaaatgaga aagtaatcaa tatctgtaaa
    11281 gcagtagtta aagaaacttt aatccaagac atttaggaga aagaaatgaa gaaatcaaga
    11341 aaattagcca cattaggaat ctgttcagcc ttgtttttag gcttggctgc ttgtcaacaa
    11401 caacatgcta cttctgaggg gacgaatcaa aggcaaagca gttcagcgaa agttccatgg
    11461 aaagcttcat acaccaacct aaacaaccag gtaagtacag aagaggtcaa atctctctta
    11521 tcagctcact tggatccaaa tagtgttgat gcatttttta atctcgttaa tgactataat
    11581 accattgtcg gctcaactgg cttatcagga gatttcactt cctttactca caccgaatac
    11641 gatgttgaga aaatcagtca tctctggaat caaaagaagg gcgatttcgt tgggaccaac
    11701 tgccgtatca atagttattg tcttttgaaa aattcagtca ccattccaaa gcttgaaaag
    11761 aatgaccaat tgcttttcct agataacgat gcgattgata aaggaaaggt ctttgattca
    11821 caagataagg aagagtttga tattctattt tcgagagttc caactgaggc aactacagat
    11881 gtcaaggttc acgctgaaaa gatggaaaca ttcttctcac aatttcaatt caatgaaaaa
    11941 gctcgaatgc tgtctgtagt cttgcacgac aatctggatg gcgagtatct gtttgtaggc
    12001 catgttgggg tcttagtacc tgctgatgac ggtttcttat ttgtagagaa attgactttc
    12061 gaagagccct accaagcgat taaatttgct agtaaggaag attgctacaa gtatttgggc
    12121 accaagtatg cggattatac aggcgaggga ctggctaagc cttttatcat ggataatgat
    12181 aagtgggtta aactttaata gtgttatgat atgacagtaa tcttaggatt tttatttcta
    12241 tttttgatga cttggtgggt tatttcaatc gttaatagcc agaaaccacg caaaaatgaa
    12301 acatttattg gctatatcca aagatacgat attgatggga atggctatca atcattaccg
    12361 tgtcctcaga actgagagga gttcttgaaa ccgtaaaacc gctttgaaca agagttactt
    12421 caacccattg gctccgacgg agtaagttgc tttcgtgaat accaaaatca gccgcaattt
    12481 gttcataagt tcgatattct cgcacatatt gaagagtggc cataagaagg tcttctaggc
    12541 ttaatttagg ttttcgtcca ccttttgcgt gtttaagttg ataagctgtt tttaatacag
    12601 ctaacatctc ttcaaaagtg gtacgctgaa caccaacaag acgcttaaat cgtgcatcag
    12661 ttagttgttt acttgcttca taattcatag aactactata ccatattttg tttcgcagga
    12721 agtctaatgc taaaatctta tcagtgagaa gatttcttaa agtttattct ttgtagtttc
    12781 atctgtatta gtacataatt taacttagag aaaataggag gactaatcga atggaattaa
    12841 aagattttac agaaaaagaa caggaaatga ttaagaaaag gcttacaatg tctaatatta
    12901 gtgacaaaga aactactgag aagattctgg cgctagtccc acaagacttg attaagcgaa
    12961 tcccgttttt tgtcagaaaa catgctacaa cacgtacgat taaacgtatt tcaattgaat
    13021 accctgaact ctacgctgta gctcaaacaa gtggtgagat tccagaaaaa aacgcgaaga
    13081 attgcgtcag attatcacaa ctatctttga acaaaagatg aataagcata gtattaagta
    13141 gagaatgaaa aaatatttta ttggcggttt gggaagcaat gcctatcata gcaaggattt
    13201 tcttcaagaa ctagattcgc aggtctattt tctaaatcca tatgaaaagc atcttcgaga
    13261 tgaaacagaa ttgaaatcat ggtttaaaaa tgagattgta gaggaagaat ctatctgtct
    13321 gataggtcat tctattggag gcgatttagc tcgttatttc gcatcggaat ttgaagaagt
    13381 aaagaaactg attcttttgg atggtggcta tctagattta gataagattt tacctttgga
    13441 tacagagtta gaggaaacta aaaattatat caaatctcaa attatttcgg acttagatgt
    13501 tcttacttct aaagaaaaat ctgaagcaaa gcattggtca gaaaatatgg agaaagctgt
    13561 aagacagtcc tatcactgga atgttgagta taatagatat gagttggcta taaattatga
    13621 aaatatagaa gcgatactcc gcctacggag aaaaatacaa gcttttaaga gagaagtggg
    13681 agataccttg tttattagtc ctcgctatcc taatgaagct acatggagag aggaagccct
    13741 aaaagaattg ccagactatt ttgatactat ttttttagaa aactttggcc atgagcttta
    13801 tactcaagca cctaaagaaa tcgctagtct aatgaatgag tggctcgctt attttctatg
    13861 agctacttgt attgctggaa atggtataag atgaaaatct acaagtcact aattttcata
    13921 taacaaaaaa cgaacaggct ctttttctgt tattcagaag agaacttgtt cgtttttatc
    13981 ttgattttct taaaaagctc tatatacata gggattttct ggtttatcga ctttggtaaa
    14041 gctgtcgatt tccaaggttg gaaggttttg tttgagttct ttatggtagt gattgaccag
    14101 ttttcctttg gctgttatcc aagtgttgtt ttcatactgc cgggtattgc ccttggtcag
    14161 caatccatag acaccagaat ctgcgataca gtggataatg ccgaatcgga acagaaattg
    14221 actattggca tgactggggt cgttatagac aaagcctgta aactggattg tcttgccctc
    14281 aaactcatct ggatagtcgt agatagcctc catgacttcc atatagtttt cattagtgat
    14341 ctgaatacta tcttgggata agtatttatc cgccgccgtt cgcatttcct tttcataggc
    14401 tgattttgaa aaataagaac tggtatctgg tttcaaatat tggcttgtcg tcccttcgct
    14461 tgtctgaatg gctagatctg ttccttccga tagggggaaa tgataacctt tagcagaaac
    14521 ggtctgagaa tccaagctaa cagttgggaa agttaagccg atgacaagtg gaatagccag
    14581 aagagaaata ctcgtcatct tggccaatcg gctgttcaga tgactgtggg ttttgacttg
    14641 cttcatccag atatacaatt gaacaattgc caagataaag gaaagcacca tggagatata
    14701 ggccagatag gaatagtgca tgttgatgta ctggtttagt ttgcccgaca gatggaggta
    14761 aatagtcagt tcaaaatagc cagctaaaac taaaaatcga atcataggat gactccaatc
    14821 aagagagaat agactaagac gacaagagtt acaatcgtta tgaagtgact gataaatcgt
    14881 gctttcaagt aatttttcat catgagaata tttttgatat ccagcattgg accaatgacg
    14941 agaaaggcca gaactggtgc caaaccgaaa ctcgagagaa gagaagcacc tataaaggca
    15001 tccgcctcac tacagagcga aagaagaaag gccaaaatca tcaagagcag gatggcaaaa
    15061 agaggagtcg cactgataga ggtcagaatc cgagtcggaa cgtagacctg tattatagaa
    15121 gcaaagaggc agccaaatac caaataacgc cccgtatcaa aaaattcatc aatggcctgc
    15181 acaaagactt gaaaaacttt ttttgcagaa ctcaagtaag aaaaatcatg ctcatgacaa
    15241 gccagacgat tttctttctg aatcggttct tgccagaaaa atcctagaaa aattcctagt
    15301 attacagcca caagaatgga acccagagct cgtaataggg cgacatggaa ggagttgcca
    15361 aaggcagaat aggtcgcaaa aagaacaatg ggattgataa caggtgctgt cacaagaaaa
    15421 ggaacggctg tgtaactggg aacctttttt tccagaaaac gattgatgat ggggacgatt
    15481 ccacattcac aagaagggaa aagcataccg acaaaggtcc caaaaaagat tctcccccaa
    15541 cgatttcgag ggagaaaatg ataaaccttg tcaggtgtga tataaacttc aatcagccct
    15601 gagacaatgc ttcctattag aacgaaggga agggcttcaa tgataatgga gagaaaaatg
    15661 gctccagttt gaagtacact agatgggagt tgttgaaaag cagtcatcta gtttttcttt
    15721 tctgctttgg cttttttctc tttgtcatca gggaaagtaa cttgtttcaa atcagggagt
    15781 ttagcaaact cttcaaacat cttgtccaag tcgtctcgtt ttgtaaattt tacagccata
    15841 ggtgtacctc gattctaaag tctacctcta ttatactatt attttgcgga aaagggagtt
    15901 ttttaatttg ttttcgatag ttgatacact tcgaaaatct cttcaaacca cgtcagcttc
    15961 gccttaccgt atatatgtga cttatccaca acctcaaaac agtattttga gcagcctgcg
    16021 gctagcttcc tagtttgctt tttgattttc attgagtatg aaaaggattg gagaaagcct
    16081 tccagcttgt ggtataatag atttatggat aaaaaatatg aaaaaatctc tcaggatttg
    16141 ggagtgatgt taaagcaaat tgataccgtt ctaagtttga cagctgaagg ggcgactatt
    16201 ccctttatcg cgcgttatcg caaggacatg actggtagtc tggatgaggt ggcgattaag
    16261 gctattattg atttggataa aagtctgaca aatctcaatg accgtaagga agctgtctta
    16321 gctaagattc aagaacaagg taagttgacc aaggaattgg aagaagctat cttagttgcc
    16381 gaaaaattag cagacgttga agaactctat cttccttata aggaaaagcg tcgtaccaag
    16441 gcaaccattg cccgtgaagc tggactcttt cctcttgctc gtttgatttt gcagaatata
    16501 gttgacttag agaaagaagc tgaaaagttc gtctgtgaag gatttgcgac tggcaaggaa
    16561 gccttgaccg gtgcagttga tattttggtc gaagccttat cggaagatgt gaccttgcgt
    16621 tctatgactt atcaggaagt gctgagacac tctaaactta cttctcaagc caaggatgaa
    16681 agtcttgatg aaaagcaggt ttttcagatt tattatgatt tttcagagac agttggaact
    16741 atgcaaggct atcgtacctt ggctctcaat cgtggggaga aacttggtgt cttgaagatc
    16801 ggttttgaac atgcgacgga ccgtattctt gccttctttg ctactcgttt caaggtgaaa
    16861 aatgcttata ttgatgaagt tgttcagcaa tccgttaaga aaaaggtctt gcctgctatc
    16921 gagcgtcgta ttcggacaga attaactgag aaagctgaag aaggagctat ccaacttttt
    16981 tctgacaatc tgcgcaatct cctcttggtt gctccactga aagggcgcgt ggttcttgga
    17041 tttgacccag cctttcgtac aggtgccaag ttagctgtcg tggatgcaac aggaaaaatg
    17101 ctgacaactc aggttattta tcctgttaaa ccagcatcag ctcgtcaaat cgaagaagcc
    17161 aagaaagatt tagcagattt aattggtcaa tacggtgtag agattattgc cattggaaat
    17221 ggaacggcca gtcgtgaaag tgaagctttt gtagcggaag ttctgaaaga tttccctgaa
    17281 gtcagctatg ttatcgttaa tgaaagtggt gcttctgtct attctgccag cgaacttgct
    17341 cgtcaggagt ttccagactt gaccgttgaa aaacgctctg ccatttctat cgcccgtcgt
    17401 ttgcaagatc ctcttgcgga attggtcaaa atcgatccta agtcaattgg tgtcggtcaa
    17461 taccaacacg atgtcagtca gaagaaacta tctgagagtc tggactttgt tgtcgataca
    17521 gtggttaacc aagttggtgt caatgtcaat acagctagcc cagctcttct ttcacacgta
    17581 gctggactca acaaaactat ctctgaaaat attgtcaaat accgcgagga agaaggaaaa
    17641 atcacttcac gcgcccaaat caagaaagtt cctcgtctgg gagccaaggc ctttgagcag
    17701 gctgctggtt tccttcgtat ccctgaaagt agcaatatcc ttgataatac aggagttcac
    17761 ccagagaatt acactgcagt taaggaactc ttcaaacgtt tagatattaa agacctaaac
    17821 gaagaagccc aaagcaaact caagtccctt tcagtcaagg aaatggctca agagctagac
    17881 ctcggtccag aaacccttaa agatatcatt gcagatcttc tcaaaccagg tcgagatttc
    17941 cgtgattcct ttgacgcacc tgtgcttcgc caagatgtcc tagatatcaa agacctagtg
    18001 gtaggtcaga agctagaagg tgttgtgcgt aacgttgttg acttcggtgc cttcgttgat
    18061 atcgggattc acgaggacgg cttgattcat atttctcata tgagtcgcaa gtttatcaaa
    18121 caccctagtc aagtggtgtc agttggagat ttggtgaccg tttgggtcaa gaaaattgat
    18181 actgaacgtg aaaaagttaa tctgtcgctc ctcgctccaa atgaaactga ctgattatgt
    18241 caagcaggtt tcactagaag acttcgggag agcttttatc catcatgtcc agtggaatag
    18301 gcgtctacgt tcgacaggtg ggcgattttt ccccaaagat gggcatctgg attttaatcc
    18361 caaggtttat cagaaactgg gtatggaggt ttttaggaaa attgtccgac atgaactctg
    18421 tcattatcac ctttatttcc aagggaaggg ctatcaacac aaggatcggg attttaagga
    18481 actgttgaaa gcagtggatg gattaaggtt tgtaccatcc ttgcccaata gtaactctaa
    18541 accactcaag ctttatcgtt gtcaatcctg ccagcaaagg tatcagcgca agcgtaggat
    18601 tgataccaaa cgctatcgct gtggactttg tcgaggtaaa ttgcttctga taaatcagcc
    18661 tgaggactga tgaaagccga gcctccccgt gatatactat gtctaaccta gcagaaagag
    18721 gaaatatcat gaacagtaaa ttttataaaa tgagacgcaa tcgcatgatt tcaggagttc
    18781 tggcaggtct gtcagacaag tggaattttg atgttaccct agttcgtttc ttgtttgcca
    18841 tttttacagt tgcaaacttt ggacttggcg tgattatcta tatcatccta gcttctatca
    18901 tgcctaccaa ggaagaaatc gaagcagaaa tgtacggaac aggcccacgt aaacgcaaag
    18961 aagcccaagc cattgacgac aatgaaggct ggttttggtg aggttctaca gaagatttgg
    19021 aataagcaaa aagctttaaa tcaatagtct aaactgcttg atttaaagct tttttacttt
    19081 tatactcaat aaaaatcaaa gtgcaaacta ggaagccagc cacaggctgt aatttagtcg
    19141 gcaatgtgaa gttgacatag tttgcacttt gatttttgaa tagtattaat aatatttcgc
    19201 cagcttcatc cacaaactat agtggattgg aactagaata gtacacctct acttctaaaa
    19261 cattgttaga aatcgatttg actatcctga tcgatttgtc ctgttcttat ttcattttat
    19321 tataaaagca gtaatttaaa ctcagttttt taatctattt ttgctgctga gacaatacat
    19381 ttttggaaaa atggtggatg ctgataacaa ctgataacca gcaaataaca taagcagtgc
    19441 cggtaaattc tgtaccgata aagtgtgtgt gttcaactaa gagctgaccg atgaatggag
    19501 agagcagata gagaagtccg atgagtatat gttctttgtg tttcattaaa gcgcaccttc
    19561 tttccttata ggttgaacga cttgcaagtg ttaaattgaa tggtatgtta tctaggtaag
    19621 cttactcaaa tgatagttcc cgcttcctcc tttccaaagt aagccttttc atacttatat
    19681 tttacaactt taaaaatagg aaatcaaatc attttctaag taattttgct tatatttcac
    19741 ccaatcttac aaaaatgtaa gattaaagtc tcttttgtaa ggttaggtta tggcaagcgg
    19801 tctttttttg atgtaaacta gactcataaa gaacaaagga gtaacatcat gaaacaaatc
    19861 ttacataaga aaactagaaa accaagtcac aaagaaattg agcgcgttca gctgggatgc
    19921 actatgatgc aagctacatt tcatttgatg ggatattaag aaaggagatt ctcatgacac
    19981 ttttagatgt aaaacacgtt caaaaaattt ataaaacacg ttttcagggc aaccaagtag
    20041 aagccctcaa ggatattcac tttaccgtcg aaaagggtga ctacgttgcc atcatgggtg
    20101 agtctggttc tggtaaatca actcttctca atattctagc tatgctggat aaaccaagtc
    20161 gtggtcaggt ttacttgaat ggaactgaca ccgcaaccat taaaaattca caggcctcta
    20221 gcttccgtcg tgaaaagttg ggctttgtct tccaagactt taacttgcta gatactctat
    20281 ctgtaaagga caatatcttg cttccgcttg tcttgtcaag aagacctatt acagagatga
    20341 tgaagaaatt ggtggtgaca gctgagaatc tgggaataaa ccaattacaa gagaagtacc
    20401 cttacgagat ttctggtggc cagaaacagc gtgtagcagt agcacgcgcc atcatcactg
    20461 aacctgaaat tctccttgcg gacgaaccga caggagctct tgactccaag tcatctgcag
    20521 ccttacttga tgtctttgat gaaatcaatg agcgcggcca aaccattctc atggtaaccc
    20581 actcaacagc agcagctagc agagctaaac gtgtactctt tatcaaagac ggcattcttt
    20641 acaatcaaat ctaccgtgga gagaagacag aacgtcagat gttccaagaa atctctgata
    20701 ccttgactgt catggcaagc gaggtgaatt agtatgtttc gattaaccaa taagttagcg
    20761 gtatcgaact tgattaaaaa ccgcaaactc tactatcctt ttgcgctggc tgttctcttg
    20821 gcagtcactg tcacctatct cttttactct ctaaccttca atcccaagat tgctgaaatt
    20881 cgtggaggaa caaccattca ggctacactt ggatttggta tgtttgtcgt cacccttgcg
    20941 tcagccatta tcgttctcta tgccaatagt tttgtcatga agaaccgttc caaggaacta
    21001 ggaatttatg gcatgttggg cttggagaag cgtcatctta tcagtatgac ctttaaggag
    21061 ttagtggtat ttgggattct aactgttgga gcgggtatcg gtattggagc cttgtttgac
    21121 aagttaattt tcgctttcct gctcaaacta atgaaattga aggttgagct ggttgctacc
    21181 ttccagacga aagttgtcat tacagtgctt gttgtcttcg gtttgatttt cctaggcctc
    21241 atgttcctga atgcccttcg aatcgcccgt atgaatgccc tccagctctc tcgtgagaaa
    21301 gctagtggag agaaaaaagg tcgcttcctt cctctccaaa ccattcttgg ttccataagt
    21361 ttaggaattg gctattatct tgcccttacg gtaaaagatc ctcttacagc cttaacaacc
    21421 ttcttcatag ctgttttact ggttatcttt gggacttatc tcttgtttaa tgcagggatt
    21481 accgttttcc tccaaatctt aaagaaaaat aagaaatact attaccaacc aaataatctc
    21541 atatctgttt ctaacttgat tttccgtatg aagaaaaatg cagttggact agccaccatc
    21601 gctatcttgt caacaatggt tttggtaacc atgtcagcag cgacaagcat tttcaattcc
    21661 tcagaaagct ttaaaaaagt tctaaatcct catgattttg gggtttcagg gcaaaatgtt
    21721 gaaaaagaag acttggacaa actcttgagc cagtttgcaa gtgacaaagg ttatagtgtc
    21781 aaagagaaag aagtacttcg ttacagtaac tttggtattg caaatcaaga aggaactaag
    21841 ttaactattt ttgaaaaagg acaaaaccgt gtccaaccaa aaacagtttt catggtattt
    21901 gaccaaaaag attatgaaaa tatgactggt caaaaactgt ctctatcagg aaatgaggtc
    21961 ggactctttg caaagaatga gggagttaaa gaacagaaag ctctaactct aaatgatcat
    22021 caattttctg taaaagaaga atttactaaa gattttattg tcaaccatgt tccaaatcag
    22081 tttaatattt tgactgctga ttacaattac cttgttgtac ctgatttaca agcctttttg
    22141 gatcaattcc cagattcggc tatctataat cagttttacg gtggtatgaa tgtaaatgcc
    22201 agtgaagcag aacaactcaa ggtcgctgag gagtatgaaa aatacttaca aaagtttaat
    22261 gctcaattaa acactgaagg taactatgtg tatggtagca ctctagcaga tgctagtgct
    22321 cagatgagtg ccctctttgg tggtgtcttc tttatcggta ttttcttatc cattatcttt
    22381 atggtcggaa ccgttctggt catctactac aaacaaattt ctgaaggata tgaagaccgt
    22441 gagcgcttta ttatcttgca gaaagtcggt ttagatcaaa agcaaatcaa gcaaaccatc
    22501 aacaaacagg ttttaactgt attcttcctt cctttgctct ttgccttcct acatctagcc
    22561 tttgcctacc atatgcttag tctgatttta aaagtgattg gtgtactgga tacgactatg
    22621 atgttgattg tgaccttgtc tatctgcgct atcttcctca tcgcctatgt gctgattttc
    22681 atgattactt caagaagtta tcgcaagatt gtgcaaatgt aaaaaagata cctcgacttc
    22741 aaaatcgagg tatttcttgt attctaaatg ctgaaaagtt gttcgagcag gaaggtaact
    22801 cccatggtca agagaccaat agcaaggttc cgaatcatag ctgttttggt tggggctttt
    22861 ccaagtctag cacttgtgta accagtgaga agaagggcca caccgacaat aaggacggta
    22921 gcagggatgc ggtaatcact tggaaaaatg gtcactgaca gcattggagg caaacttcta
    22981 aggaaaaagg caacgaagct agaaatggca gcgtgccaag gattggtaaa ttcttcatac
    23041 tcaatcccat atttttcctc taccagagcc ttgagtggat ttttaagaaa gatcttatta
    23101 gtcaagagtt gggcagaagt tttgaattct tcattttgga tataagcagc atagagggat
    23161 tttttggcta gttccctatc ttggtctagc aagagttttt ctcgcgaaac ggcagcttcc
    23221 tcggtatctt ttggagttga aacggataca tattctccac cagccattga aaaggcacca
    23281 gctaagatag ccgcaaatcc tgataaaaag ataatccaga tattggtcgt ggcactggca
    23341 accccgataa ccacaccagc aatggaaata attccatcgt tagcatcaag aacacccgca
    23401 cgcaggatat ttaaacgacc tgcaaaattt gaatcaattt cgtgatttgt ttctgacgct
    23461 aaatttcaag ttcaagttag ccatcca
//
LOCUS       DAVBBS010000031        23482 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVBBS010000031 DAVBBS010000000
VERSION     DAVBBS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23482
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.031"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(1..319)
                     /locus_tag="VQU32_001197"
                     /pseudo
     CDS             complement(1..319)
                     /locus_tag="VQU32_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(395..796)
                     /locus_tag="VQU32_001198"
     CDS             complement(395..796)
                     /locus_tag="VQU32_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2472657.1"
                     /translation="MDSASIIAHTIHQKFNLKVPNYRQEEDWARMGLPITRKEIANWH
                     IKASQYYLESLYNLLREKLLEQPLLHADETSYRVLESDSQLTYYWTFLSGKSEKQGIT
                     LYHHDQCRSGSVVQEFLGDYSGYVHCDMLRQ"
     gene            complement(804..1109)
                     /locus_tag="VQU32_001199"
     CDS             complement(804..1109)
                     /locus_tag="VQU32_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HET2472658.1"
                     /translation="MFPVETEEITYKRKKSKGKRQALLAQFDSEEVHHQVEESICSDC
                     QGDLKEIGATLQRQELVFIPAQLKRIDHIQHAYKCQACSDKNPSDKIVKAPIPKAPL"
     gene            complement(1096..1290)
                     /locus_tag="VQU32_001200"
     CDS             complement(1096..1290)
                     /locus_tag="VQU32_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2472659.1"
                     /translation="MKIIQQQSATIDSLTNALALLREQVAYLTQKLYGKSSEKSVCPS
                     GQLNLFEEESPSEKDGDVPS"
     gene            complement(1366..1706)
                     /gene="tnpB"
                     /locus_tag="VQU32_001201"
                     /pseudo
     CDS             complement(1366..1706)
                     /gene="tnpB"
                     /locus_tag="VQU32_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(1687..1872)
                     /locus_tag="VQU32_001202"
     CDS             complement(1687..1872)
                     /locus_tag="VQU32_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472660.1"
                     /translation="MPQPIVPVEIPQSRPFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
     gene            complement(2176..3738)
                     /gene="guaA"
                     /locus_tag="VQU32_001203"
     CDS             complement(2176..3738)
                     /gene="guaA"
                     /locus_tag="VQU32_001203"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET2472661.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            3880..4578
                     /locus_tag="VQU32_001204"
     CDS             3880..4578
                     /locus_tag="VQU32_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2472662.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(4621..5517)
                     /locus_tag="VQU32_001205"
     CDS             complement(4621..5517)
                     /locus_tag="VQU32_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET2472663.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLLIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(5526..6461)
                     /locus_tag="VQU32_001206"
     CDS             complement(5526..6461)
                     /locus_tag="VQU32_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HET2472664.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(6458..7183)
                     /locus_tag="VQU32_001207"
     CDS             complement(6458..7183)
                     /locus_tag="VQU32_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HET2472665.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(7267..7902)
                     /locus_tag="VQU32_001208"
     CDS             complement(7267..7902)
                     /locus_tag="VQU32_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HET2472666.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(7904..8731)
                     /locus_tag="VQU32_001209"
     CDS             complement(7904..8731)
                     /locus_tag="VQU32_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2472667.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(8753..9089)
                     /locus_tag="VQU32_001210"
                     /pseudo
     CDS             complement(8753..9089)
                     /locus_tag="VQU32_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
     gene            complement(9090..9806)
                     /locus_tag="VQU32_001211"
     CDS             complement(9090..9806)
                     /locus_tag="VQU32_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET2472668.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNHILDSQIEGVISMADII
                     EKAGITPIGVYTHEPRDQAPLVIKEVNAIKVALLAYSYGFNGIEQYIFQEDYNRYLSD
                     LNEDKIKAEVERAEKEADITIIMPQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSKTVEKDGDKKLIIY"
     gene            10329..10940
                     /locus_tag="VQU32_001212"
     CDS             10329..10940
                     /locus_tag="VQU32_001212"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET2472669.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEGIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            10985..11713
                     /gene="rlmB"
                     /locus_tag="VQU32_001213"
     CDS             10985..11713
                     /gene="rlmB"
                     /locus_tag="VQU32_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET2472670.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFLNRL"
     gene            complement(11752..12663)
                     /gene="trxB"
                     /locus_tag="VQU32_001214"
     CDS             complement(11752..12663)
                     /gene="trxB"
                     /locus_tag="VQU32_001214"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET2472671.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVKEIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(12729..12953)
                     /locus_tag="VQU32_001215"
     CDS             complement(12729..12953)
                     /locus_tag="VQU32_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HET2472672.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(13031..13774)
                     /locus_tag="VQU32_001216"
     CDS             complement(13031..13774)
                     /locus_tag="VQU32_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2472673.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILLLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(13774..14574)
                     /locus_tag="VQU32_001217"
     CDS             complement(13774..14574)
                     /locus_tag="VQU32_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2472674.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(14732..15088)
                     /locus_tag="VQU32_001218"
     CDS             complement(14732..15088)
                     /locus_tag="VQU32_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET2472675.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(15101..15613)
                     /locus_tag="VQU32_001219"
     CDS             complement(15101..15613)
                     /locus_tag="VQU32_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET2472676.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(15613..16107)
                     /locus_tag="VQU32_001220"
     CDS             complement(15613..16107)
                     /locus_tag="VQU32_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2472677.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            16242..16688
                     /locus_tag="VQU32_001221"
     CDS             16242..16688
                     /locus_tag="VQU32_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HET2472678.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            16685..17332
                     /locus_tag="VQU32_001222"
     CDS             16685..17332
                     /locus_tag="VQU32_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET2472679.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(17353..17934)
                     /gene="pdxT"
                     /locus_tag="VQU32_001223"
     CDS             complement(17353..17934)
                     /gene="pdxT"
                     /locus_tag="VQU32_001223"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HET2472680.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(17935..18810)
                     /gene="pdxS"
                     /locus_tag="VQU32_001224"
     CDS             complement(17935..18810)
                     /gene="pdxS"
                     /locus_tag="VQU32_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HET2472681.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(18962..20341)
                     /locus_tag="VQU32_001225"
     CDS             complement(18962..20341)
                     /locus_tag="VQU32_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET2472682.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKDKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            20636..21559
                     /locus_tag="VQU32_001226"
     CDS             20636..21559
                     /locus_tag="VQU32_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET2472683.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGISPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            21619..22224
                     /locus_tag="VQU32_001227"
     CDS             21619..22224
                     /locus_tag="VQU32_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2472684.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKTIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLTHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            22242..>23482
                     /locus_tag="VQU32_001228"
     CDS             22242..>23482
                     /locus_tag="VQU32_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2472685.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRNFKFKFELEVLEIDEVPMF
                     NQDEKWDESFQLRFLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGAS"
BASE COUNT         6972 a         5279 c         4282 g         6949 t
ORIGIN      
        1 atacttgagg ctgtattcaa ttgcccttcc tagttttgaa cccgataaaa ctgactgacg
       61 ccggcaccaa gcaaagaaat cttccattag gttggagctc ttcttgacgt ttctgtagtc
      121 gttcatcagc tggcaaagcc tcccagtctc tttccaagaa aaataactga tcacaataag
      181 ctaaaccttt agctcctaag gatgatttat ccgcttgctt gggggtcgct tcaaaaaact
      241 tccttctcac atgcgcccaa catccaacaa gtgcagcttc ttccagttga cgataagctt
      301 ctagcggttc gcagtttacc aagcttagcg tcgccttgct cctaaacctt ggactgctat
      361 cgcataggca gaactagagg actaaagtcc taagttactg ccgcaacata tcacaatgca
      421 catagccaga ataatctcct aggaattctt gtactactga accacttcga cactgatcat
      481 ggtggtaaag cgtaatccct tgtttctctg atttacctga caaaaaagtc caatagtaag
      541 tcagctgact atcactttct aagacccgat aagaggtttc atctgcatga agaagaggtt
      601 gttctaacaa cttttctcgt aaaaggttat aaagggactc taaatagtat tgactcgcct
      661 tgatatgcca attagcaatt tccttacgtg tgattggtaa acccatccta gcccaatctt
      721 cttcttggcg ataattaggt accttcagat taaacttctg atggatggtg tgagcgataa
      781 tagaagctga gtcaatagtc tcgttataaa ggggctttag gaataggagc tttcacaatt
      841 ttatcactcg gatttttatc actgcatgct tggcatttgt aagcgtgttg gatatgatct
      901 atgcgtttta attgcgcagg aataaagact aattcttgtc gttgaagggt tgctccaatc
      961 tcttttagat ctccctgaca atcagagcaa atgctctctt ctacttgatg atgaacttct
     1021 tctgaatcaa attgggcaag aagagcttgg cgtttccctt tagatttctt acgtttgtag
     1081 gtaatttcct ctgtttcaac tgggaacatc tccatctttc tcagatggag actcttcctc
     1141 aaaaaggttg agttgtccag aggggcaaac acttttctca gaggattttc catagagctt
     1201 ttgagtcaga taagccactt gttcacgaag aagggcaagt gcattggtga gactatcaat
     1261 tgtagcactc tgttgttgaa tgattttcaa tcttcttcca tgggcttccc ctccttgttt
     1321 tctaccttta ttatactaaa aagaaagtcc tcatttcaat agaaatcacg actttctgat
     1381 ggatttattt ttggagtgat agaaaagtcc ttcataagcc agtctacttg ttcagatgtg
     1441 agagctttga catccttttc tgtactgggc caagtcagtc tgccgttccc aaagcgttta
     1501 tatagtagcc aaaatccttg accatcccag taaagggctt taaagcggta tttacgtcca
     1561 ccacaaaaga gaaaaacttg acctgagaaa ggatctaatt caaagtgggt tttaaccaga
     1621 taagccagtg aatcaatgcc ttgcctcata tccgttttcc cacatacgag atagacctgc
     1681 cctaggctag atagatgaat tgtcatagag caacacctta tccaaaagct gttctaccat
     1741 ttcgagattg agagattgaa aaaaacttac ttcaagcttg ccaatacgaa ttttaagcag
     1801 aatatcattt ctctttttag aatcaaaagg acgagattgt ggaatctcta caggaacaat
     1861 aggttgtggc attaaaatat cctccaatag ttttacttta ctaatagtat acaggaggaa
     1921 ctccatgaat gatagatacc ttgttatgac gcgattacta tattagaaag ttggctctct
     1981 aattaaatta attctcatta tatatatcaa aagaccgcag cttgaaaact acggtctaat
     2041 tttttgtcca aatgtaaacc acttggaact attattcaat ttattctttt tagtaaatgt
     2101 tcccgtttta atcccagtta cagtttttaa tcctctgtaa ccgttgattt tacaaagctt
     2161 ttaatttttt tgcgattatt cccactcaac tgttgcaggt ggtttacttg taatatcgta
     2221 gacgatacgg ttaacatgat ccacttcatt tacgatacgt actgagattt tttgaagtac
     2281 ttcccatgga attttggcaa aatcagcagt cataccatcg atagaagtga tagcacggat
     2341 tgcaatcgtg tagtcatatg tacgaccgtc acccataaca ccgactgaac gaacgcctgt
     2401 gttaacagtg aagtattgcc aaatatcgcg gtcaagtcca gctttagcga tttcttcacg
     2461 aagaatagcg tctgattcac gaacggtttc aagtttctct tcagtgattt cacccatgac
     2521 acggatagca agtcctggtc ctgggaatgg ttggcgccat acgatatggt ctggcatacc
     2581 aagctctgta ccaagagcac gaacttcatc cttgtaaaga gtattgagtg gttcaatcaa
     2641 ttcaaactgc atatcttctg gaagaccacc cacgttgtgg tgtgacttga tagtttgagc
     2701 tgtatccgta ccagactcga taacatctgt atataaagta ccttgagcaa ggaatttcac
     2761 atctttgagc ttgcttgctt cgtcatcgaa tacatagaca aactcgttac cgatgatttt
     2821 acgtttttgt tcaggatcag aaacgccagc aagtttgtca aggaaacgtt tagcagcgtc
     2881 tgctttgacg atattcaaac caaacttacc accgagcatg tccataactt gatcagcttc
     2941 gcctttacga agaagaccgt ggtctacgaa gatacagatc aattgatcgc caatcgcttt
     3001 ttggagaaga accccaacga cagatgagtc aacaccacct gatagaccaa gaaggacacg
     3061 tttatcaccg acggtttcac gaattttttt gatctgcatg tcaatgaaat tatccattga
     3121 ccagtcacct ttagccttac aaatgttaag ggcaaagtta cgaaggatat catttccgta
     3181 tacagaatga cgaacttctg ggtggaattg gataccgtaa atgtgtttat ctgggttttc
     3241 gatggctgcg tatgggcagt cagctgatgt acctgtacga acaaagtcag caggaatctc
     3301 agtaaccgca tcaccatggc tcatcaaaac agtctgttca tcaggtgttg attcaaaaag
     3361 cgctgatggt gtgtgagtta gggttgattg accgtattca cgatttccag catcacctgc
     3421 aggaacaact tttcctccaa gtttatgggt caataactgc ataccataac agattcccaa
     3481 aattggaatt ccgagttcga agatttctgg gtcaatatca aatgaaccat cttcatatac
     3541 agaatttgga ccacctgata gaataattcc tacaggattg acttcacgaa cttcagcagc
     3601 tgaaatttta tggcttttta gttctgaaaa aacaccaatc tcacggatac ggcgtgaaat
     3661 cagctggttg tactggctac catagtccaa tacgatgatt ttttctacat cttgcaaatc
     3721 agttgaaatg ttgctcatct ttttcctttc tcaaaagaaa tatcttttct caatattcta
     3781 tcacaaataa gggcgaatta ccaactaaac aaggcgaagt ttgacttttt ctagtttatt
     3841 ggggtacaat agagaaaaga tagaaatgaa gtgaaaaata tgctacctgc ttatatgaaa
     3901 atccatgatc agataaaaaa ggatattgac gagcaccgtt gggctattgg tgagaggctt
     3961 cccagtgaaa gagatttagc tgagcagttt gcggtcagtc gcatgaccct ccgccaagcc
     4021 gtatctctat tagtcgaaga aggcgtctta gagcgccgtg taggaagcgg cacctttgtt
     4081 tccagtactc gagtacaaga aaagatgcga gggacaacca gttttactga aattgtcaaa
     4141 tcccaaggta aagttccctc tagccagctc atttcctaca aaaaaaccat tcccaatgag
     4201 caagaagttg ccaagctagg aatttttcca acggacaata ttatccgaat ggagcgggtc
     4261 cgctatgccg accaagttcc cctagtttat gaagttgctt ctattcctga aaaattcatt
     4321 aaggacttta aaaaagaaga aatcacaagt catttcttcc aaaccttgca aaaacatggc
     4381 tatcgtatcg gcaaatctca acagaccatc tatgctcgcc ttgctaaaga aaagattgcc
     4441 cactatttgg aagttgaaaa aggacatgct attcttggat tgacccaggt ttcctaccta
     4501 gaagatggta ctgcttttga atacgtaaaa agtcaatatg taggcgaacg ctttgaattt
     4561 tatcttgaaa ataattagat taccacgcaa aaactctctg tcacggacaa agagtctttt
     4621 ttatttttct aagaataaat ctttcaaaga aatcaaagtt acagcaaaca atatcattgc
     4681 cataccaaga aaatcaatgg gataaaattg ttcattcatt attaagaagg caaagaaaat
     4741 cgccgatatt ggctcaattg aagccaacaa gcttgacttg accggtccta tcagactggc
     4801 tcctttaagg aaagctgtat aggcaaagac agtcccgata aggataatgc ctgcaaacgc
     4861 aaggagaaaa tcaagactag tcgggatatc ggcctgtaga acccctgtaa aaggaagggc
     4921 gaccaaacct gctatgacca ttcccacacc aatgagcaag ctgctccccc actttttaat
     4981 caaggctatg ggtaaaatga tatacagagc ataagtcaag gcagaaaaga gaccccagaa
     5041 cagaccagca ggtgtcatgg ataactggtc caactgccca tgtgttgcga tcaggaaggt
     5101 tcctccgatg gcgaatatga tggaaactat ctctcccagt gtcggtgcca ccctatcctt
     5161 gatacagcta taaattaaaa ttccgacagg acaaacatac tgaagcaccg tcgctgttcc
     5221 cgcattggtc tcctgaatag cagacagata ggcgaattgg ttgagaaaaa gaccaatcag
     5281 agcaaaaata agaagagaca gcaaactctt tctatccttt aaaaagacca gtattttatc
     5341 ctttgcagta gcataagcca agagcatgag aattccacca gcgattaaaa gacgcaagtt
     5401 ggtcaagacc agagccgaaa ttccgtgtgc cattaggtat tggccactcg ttcctgacaa
     5461 cccccaagca ataccagcca caactgttag taaagtccct tttaaactat ttgacatgtc
     5521 tctccttact cttctttgcg aatcaagttc atcacttgat tgcggtctaa tatccattga
     5581 gctctctcgt ccttttcata ggtacgattc gataggaaaa tagtggcttc ttgcttctga
     5641 cgattccaca tgataaaggt acctgtatag cccgtatggt ctagccaatc tccttccaaa
     5701 ttccatgcta aagaacgttc cttgtcatcc aaaggagaaa aattttgatt taagtctctt
     5761 gcaaaatcat ctgctaaata gtgttctaaa aagatttgta aatcctttat agtcgaaaat
     5821 aaaccagcac tcccagcatg tctacccagg agacgagcct tgggatcatg cactatgcct
     5881 gcctctacac ctctaactgt tggaacagca agctcaactg gcccaaactt ggtttccgtc
     5941 attccccaag gtttccagac ttgatccttt aaaatcacat ccaaatcttg attaaaaatt
     6001 ctttccaaaa taaagcccaa cagcaaaaaa tggacatccg aataaagaaa ggctggctga
     6061 cttcgtctgt tgagatgaaa catcgcttcc tttaattcag gggctgttaa aagatcacga
     6121 ttaggaataa aaggatcaag gtctgttgca tgagtcaaga gctggcgaat agtgatgtct
     6181 ggataatcac tctcaggtaa aaaatctatt accagtctat caatatctaa ttgacctatt
     6241 tcccacaaga aggtacaaac tgtgccaacc ccaacaacct tgctgacact agctagatca
     6301 taaactagtc ctgcctcagt ctgcaagcca tgctctgggt cactctggcc taaatagaac
     6361 tctgtccatt gattgtcctt aaaatacgca aaagaggctc cgggataaat tcctgcctcg
     6421 atttgttctt ctattttttt aataatcttg gtccacttca tacttcttca aaccacaatt
     6481 caacattatt tctatcttta ccaaggaaga atttttcaga cttaggaata aaatattcag
     6541 tagactcaaa cttctggcga agacttgcta tgtctaattc attgaccaag aacttgagca
     6601 tagataagtc ccaggtaacc gtattgtcca cagtcaaatc ctgcccctga gctggaataa
     6661 aatcaaggga tgccccaatt tcagatgatt ccaagaaact ttcgatatca gttgggagat
     6721 gtaattccat agaaatctca aatttactta aagaaattga tgccaaatct gtttgaaatt
     6781 caggcttttc tcctacttct actagacttg ctctgtcatc ttccgcatga atcaaaatca
     6841 aatcatcttc tggtgagaaa atttcaaaag cgtagccatt ttgaccttta tataatcgat
     6901 gaatcgaatc tgttttagat aagattcctt caatttctaa gggattttcc accttgacaa
     6961 tcaatctagc tagttttttt cttccctcta ccttacgagt acgcatactg ggagcttctt
     7021 ctaaaaccag cttttcaaga cccgtttggt cacctagtga cagaaaggcc gattcttcta
     7081 acaaggcctt cattccaagg gtttcaatat aaaatgtttc atttaatttt ctattattag
     7141 cttttaaagt aggaataatc cgtacaatct gatttacatt cataaattcc tccgtcactt
     7201 tttattttaa aggattttag aattttttac aagatattat gctcaaattt aacattattt
     7261 agctatttag taaagatagt ctttcaagga ttttgacaaa gcctgataac cagcaatact
     7321 gaggtgcagt ccatcagttg tatattcttt tttgagttgg cctgcttggt ctgtcaaaca
     7381 atcaaatact ggcacaaatt ccacctgcat ataggcagat gcaagctctt gataggcttg
     7441 attccagttc tgaatttttt cattcgagcg gatatagact gcctgctggt actcctctcc
     7501 ctcattgaca ggcaaaatgg aaagcaattt aatctctgtc aatggataat cgcgagcaac
     7561 ggattgaatg atagcttcga gattattgag agcctcattc acaggaacat cctttccgat
     7621 atcatttgtc ccaatcagaa gaaaaatttt atctactgct ccaccatata gatgagcatc
     7681 aagattctct agtaacagtc ctgtctgata gccacgaatt cctcgattga caatcgtctt
     7741 tgaagtccca aatagctcct gtagaggata atactcgaca atggaatccc caataaaaag
     7801 aatgtttggt tctacaacag aaatgtgatt taggtgacga tacttagttt gaattttttc
     7861 ttgttccttt aggagccaat tttctaataa ctgtactgcc acttcattct cctttttcta
     7921 accagttttc caagacttga taaactcctc cttggctgtt ggctggtgct agagcagtcg
     7981 ccacagcttt ggctttctca tcagcatttt ccatcgcata ggcaattcca gccatttcaa
     8041 gcatttcaac atcattttca ctatcaccaa aagccatgat ttcttgggat ttcaagtccc
     8101 agcgcttgag taattcctcc aagccccatg ctttatgaat cccagcttgg aggatatcaa
     8161 tgcaaccata gccactggat acagctcgga cacggccatc aaagagagca ttgatttctt
     8221 ccaaaaccga actcaaacgt tcctcaccaa caaccatgct catcttgagc acaccaccaa
     8281 agaggtcaga tgttaattca tccacaaatt gcatccgttg gtagaatttt tcaatcattt
     8341 ctggagtcat aaaactttca agatctgtaa aaatcgtacc ttccttgaca aaatcaccct
     8401 tcatccccgt tacaacaaac tggtcctgac acgctcgacc cttgaaatga gccaaagcct
     8461 tgttgacaat ggcgtcatcc catgtctgag cctgaatcaa ttcattgttt tcaaaaatac
     8521 gagcgccatt agcaacaacc agaaccactc gatccaccaa gggactcagt agttgtctca
     8581 tgcggtgaat ttcattgccc gtcgcaatga caaaacgaat gcccctttga tccaactgat
     8641 ctaaaatctt ttccaatcgt gggagatcaa gctgacccct agcatccagc aaggtcccat
     8701 ccatatctgt cgcaattacc ttaatcatca tttactttcc tctggattca agctaatacc
     8761 acttcaatcc cacatgttca ttcatttctt tataggctgt atcaattcgt tccttagtcg
     8821 cttcatctaa ctggtcacga tgactgccac ccctctataa aatcttccaa aatataagtt
     8881 tggtaatgat acaagggata tccttctggt gaaaaggttc cctttggtgt tcgattgacc
     8941 caagtaggat gagctttagc tgttccgata gttgtttttc catccttctt cttgatggtg
     9001 acatccatga gaacaccacg ttcagtccac ttagcattct cttcatctcc catagattca
     9061 atccgttgat tggaaatgaa gttccccatt taatagataa tgagtttctt atctccatct
     9121 ttttcaaccg ttttagatgg ttcaacaacg tgaggatgcc ctccaaagat aatatccgct
     9181 ccccaatcga tcatcttgtg ataaagagct ttttgttctt cagttggttc caatcgatac
     9241 tcaacaccca tctgaggcat gataatggtg atatctgctt ccttctctgc ccgttcgact
     9301 tcagccttta tcttatcttc gtttaaatct gaaagataac gattatagtc ttcctgaaaa
     9361 atatactgct caattccatt gaaaccatag gaataggcta acaatgcaac cttgatagca
     9421 ttcacttcct taatgaccag cggagcctga tcacgtggtt cgtgcgtata aactccgatt
     9481 ggagtgattc cagctttctc aataatatcg gccattgaaa taactccctc aatttgcgaa
     9541 tccaaaatat gattatgagc taaatccagc acatgataac ctgcttcctt aatagcatcc
     9601 ataacttcaa caggagcatt aaagagaaga taacccgcta aataatgatc cttattaatg
     9661 gttccttcaa aatcaccaat agctaaatct gcttgcttga gccaaggagt cacatactca
     9721 aaattttcat gaaagtcata ggtaccgtct tcttttttag ctgaaaagaa aagtccatcg
     9781 tggtagagca aatccccgtg ggccataatt ctggcagttt tttcctcttc ctactcctga
     9841 gacttttgct tagtcccctc ttgagaaata gtatctcgtt tttgactagt caagggattc
     9901 cttggaagct ttcaaacaac aagaccaagc ccattgataa accaactgct agcaagagta
     9961 tcgccacaaa tcccttattg ctccacttgc gataactcct aaaaagttta ccaagccctt
    10021 cataaaacga aaagctaaac caccctgatt tcgattttgt cttctttgta tcttcattct
    10081 ccctactttc ttatgcaagc cttttctttt tattatatca cagataagta tttctttcac
    10141 aattgaattg aacttcccat ctattttcta taaatcctaa atgccataat gctttcaatt
    10201 cttgtcattt tgtgatatca tgtagaggta atgaaaggaa atgaactaat ccacagtggc
    10261 ttattccaag tataccactt gggctttggc agtagttaac tgcactaaac ataatataag
    10321 gagagaaaat gtctgcaata gaacgtatta caaaagctgc tcacttaatt gatatgaacg
    10381 atattatccg tgaagggaat cctactctac gcgcgattgc tgaggaagtc actttccccc
    10441 tatctgacca ggaaatcatc ctaggcgaaa agatgatgca attccttaaa cattcccaag
    10501 atcctgtcat ggctgaaaaa atgggactcc gcggtggtgt tggactggct gctccccagt
    10561 tagatatctc aaaacgcatt atcgctgttt tggtacctaa tattgttgaa gaaggcgaaa
    10621 ctccacagga agcctacgat ttggaaggca ttatgtacaa tccaaaaatc gtctctcact
    10681 ctgttcaaga tgctgctctt ggcgaaggag aaggttgcct gtctgttgac cgtaacgtgc
    10741 ctggctatgt tgttcgccat gcccgcgtta ctgttgacta ctttgacaaa gatggagaaa
    10801 aacaccgtat caaactcaaa ggctacaact ccattgttgt tcagcatgaa attgaccaca
    10861 ttaacggtat catgttttac gatcgcatca atgaaaaaga cccatttgca gttaaagatg
    10921 gtttactgat tcttgaataa agaaaatccc gttgcaagac ggggttttgt gttataatag
    10981 aggcatgaaa acaaatgata ttgtctatgg tgtccacgcc gttaccgaag ccctccttgc
    11041 aaatacagga aacaaactct acctccaaga agatctccga ggtaagaatg ttgagaaagt
    11101 caaggaacta gctacagaaa agaaggtgtc catttcttgg acatcaaaaa aatctctctc
    11161 tgagatgact gaaggtgctg ttcatcaagg ttttgttcta cgagtgtctg aatttgccta
    11221 tagcgagcta gattacatcc ttgcaaaaac acgccaagaa gaaaatccac ttctattgat
    11281 tctagatggt ctaaccgatc cccataatct gggttctatc ttgcgaacag ccgatgcgac
    11341 caatgtttca ggtgtcatca ttcccaagca ccgtgctgtc ggagtaactc ctgtcgttgc
    11401 caaaacagcc acaggtgcta ttgaacacgt tccaattgcc cgagtgacaa acctcagtca
    11461 aaccttagat aaactcaagg atgaaggctt ctggaccttt ggaacggata tgaacggtac
    11521 tccttgccac aagtggaata caaaagggaa aatcgccctc atcatcggaa atgaaggaaa
    11581 aggcatctct agcaacatca aaaaacaggt cgatgaaatg attaccattc cgatgaatgg
    11641 acatgttcaa agcctcaatg ccagtgttgc tgcagccatt ctcatgtacg aagtttttct
    11701 aaatagacta taaaaaagtt tccagtcatc tgattggaaa cttttttatg attaactatg
    11761 ttctgtgata aacttgtagg cttcttgacc agcgatagct ccatctccaa ctgctgttgt
    11821 tacttggcga aggtctttca agcgaacatc tccaactgca aagataccgt cgactgcagt
    11881 cttcatgtgg ctatctgtca caatccagcc tgcctgatct tggatattca attctttaac
    11941 aaaatcgcta agagggtcca aaccaacata gataaagaca ccaccgaagg cttgctctgt
    12001 cacttgacct gttttcacat tttcaaaaac gacagattct actctgtttt cacccttgat
    12061 ttccttcact acagaatccc agataaagct gattttttca ttcgcaaagg cgcgatcttg
    12121 taaaaccttt tgggcacgaa gttggtcacg acggtgaaca atggtaacag tcttagcaaa
    12181 acgagtcaag aagagggctt cttcaacagc tgaatctcca ccaccaacta ccaataaatc
    12241 ttggtcacgg aagaaagcac catcacacac agcacagtaa gaaacaccac gactgttcag
    12301 ttcttcttct ccaggcactc ccaaaggacg gtgtttagaa ccagttgcta cgataactgt
    12361 acgtgtttca tatgtttggt catcagtcat cactttctta aaatcaccat ggtcttcgac
    12421 attttcaaca taaccataaa tgtgctcaac accaagattt tcaagtggtt caaacatctt
    12481 ttcagccaat tcaggtccac taatattagc gtatcctggg taattttcga tatcagatgt
    12541 attattcatc tgaccacctg gcagaccacc ttcaatcaaa gctactttta gattgcttcg
    12601 agcagcatac aaggccgcag tcatccctgc aggtccagca ccgataataa tagtatcgta
    12661 catatagatt ccttctttct tgttgtaact atctttattc taactctttc ttgtcaatca
    12721 cgcaaggatt atgccttgaa aacaagaatt aaactcataa ccgcaaagga taatactggg
    12781 acaaccaaga ctccaatcat aatcatatca tagagatggg cttggcgagc cctggctgtc
    12841 ttatcaactc ccgacatggc tctcagacca atccaaatcc ctaaaaaaat cagaacaagg
    12901 atggtggtca agatcaaact ctcgaaatat aaagaaaata gttgcagtag catgatttct
    12961 ctcatttcta tcttttttaa agagtaaact cagctagtcc aactaactga gttttccttt
    13021 atctattata tcaaatataa gtccgtttgt aactagcgaa gaattctttt gtccgctctt
    13081 ctttaggggt gtggataatc tcatccggag ttccagactc gatgattttc cccttatcta
    13141 ataagagaat tttatccgca acttgggcta caaaggacat gtcatgactg accaaaatca
    13201 tggtctgacc tgacttagca gcatctgcaa tagacttttc tacttcaccg accaattctg
    13261 ggtcaagggc tgaagttggt tcgtctaaga gcaaaacatc tggtttcata gcaagcgcac
    13321 gcgctagggc aacccgttgc ttctgtccac ctgataaatg gcgaggataa tggttttcac
    13381 ggtccgaaag cccaacctta gccaactctt ccttggcaat cttagtcgct tcttggtcag
    13441 ataatttctt gacaacaacc aagccttctt tcacattatc aagtgctgtt cgacgttcaa
    13501 ataaattaaa ctgttggaaa accatagaca acttacggcg tagggcaagg atttcttctt
    13561 gagtgatttt agaaaaatca actgaaaaac catcaatctg aatagagcca ctgtcaggtg
    13621 tttcaagata attgagactg cgaagaaagg ttgattttcc agctcctgaa gaaccaatca
    13681 aggctacaac ttcccctttt tgaatatcca agttcagatg atccaagaca gtctgtcctg
    13741 aaaaggattt gcttaaattc gaaatcttaa tcattaacga aggtctcctt tcacatctgt
    13801 ttgcactgta tcaggtgcag aaatagccat ttttctctcg atgaaacgac cgaggctttc
    13861 aattccgata ttgactaccc aataaacaag ggcaacagag atgaagcgtt caaaatagcg
    13921 ataatcagct ccacctagaa tctgagcttg ggcaaagact tccacaacac ccgcactaaa
    13981 agctagagat gttcccttgg tcaaaccgat gagggaatta atcaaggttg gagtagctac
    14041 caccgctgca ttaggaataa tcactcgtcg ataaacttgc gctcgggtca tacccagact
    14101 gcgcgccgcc tcaatctcac caggattaac tgagagaatg gctgcacgaa tggtttcact
    14161 agcataagct gcctcattaa aggcaaaagc gacaatcgca aaagctgcag ctggaatcgc
    14221 attgatattg agaccagttc cccattgctg attgagggct ttcaaagcca aagggattcc
    14281 gtagtaggtc aacatgagtt gcaccaaaat cggtgtccct tttaagaaac taacaaagaa
    14341 ggcctgcaag ggatataaaa tcttgacacg attgatcttc acaatggcaa aaagaagcgc
    14401 caaaaccaag ccaaaaaggg caccaccaat tgtcaacata attgttgttg gaagttgttg
    14461 gacaattcta gggattccat caaagaccga acgtaggcta aacagcttgc catccggaat
    14521 caattgcatc aagttttggt accaatctga tgctaaaatc gttgtaacat tcataaaaac
    14581 atcctcttct gataatgatt cattctacca tgcttctgcc gtcaatgaaa ttatttgcta
    14641 cggtcagata cagactttgt ctacctacta gtttatcata ctttgaaaca gggaagttat
    14701 atattttatc tatcaaagag atagataaaa actatttcag tcccagttct tcataagatt
    14761 ttcgataacc gatttgctta acagttccat tttctactaa aatgggccgt tttaacaaca
    14821 taccgtcact tgctagcaac tcagccgctt cttggtttga caaacttcct accttatctt
    14881 ttagccctaa ttcacggtat ttgataccac tggtgttgaa aaattgcttc aattcaaatc
    14941 ctgaggtttc tagccaattc aaaatgactt cttggctagg tgtttcttcc acgatatgga
    15001 cggctttata gtccacacct aattgattta attcttgttt tgctttttta caagttgaac
    15061 atttggggta ttcgataaat tctaacatgt gcttctcctt ctatttgttt atatctttaa
    15121 aatctacgcc ttcatgctcc aagagccaag ctttcttttc cactcctgca gcatagcctg
    15181 tcagacgctt gcctgctccc aacacacgat gacaaggtac taggatagac caaggattgc
    15241 gtcccactgc tccaccaatt gcttgagcag aagccacttg caggtcttga gcaatttgtc
    15301 cataggtcac tgtctgacca taaggaatgc cctgtaaata gtcccaaact ctcttttcaa
    15361 aattcgttcc gattggcgcc aagagcaagt tggataaatc ctgaggcttg cctttaaagt
    15421 aatcatctaa gcaagcaata actgggtcta aaataggatg actaacaact tcttctatcg
    15481 tttcatctcc tagtcccctc tcaaaatgct tctgctcctg aacccaaatt ccatacaaat
    15541 aatggtcatc agctacaagt gatagaatac caattggtga ggagtagagt atttttacat
    15601 acttcttttg cattatttct ttaatttttg ataggccaag cgttcgccat ctactggcac
    15661 cttaccaact tgtttaaaac caagtttttc aaaaatatgt tgcataacct tgttttcagc
    15721 atgcgtatct gagcgaaaat caagataatc aaaaccttca atcaagccct ctaagaaggt
    15781 ttgagcaact ccttttccct gcacatctgc tgccacagca atacggtgaa agactagata
    15841 ctctgactct ccagcttgcc agtttccctc ataaatagct tcataggcct cctctggact
    15901 cttggtcaca gcagcatagg ctagcagttc tccctcttcc aaggctacgt aggcttgccc
    15961 tgagatgata tcatcgataa taacatctgc atttggatag ccattttgcc actggtcact
    16021 accagcatct gctaaacatt ttttggcatc ctccatcacc tgcatgatgg catctacttc
    16081 atttggaaaa gctaaacgaa tttccatctt ttttcctcat ttctgactag tttctcccat
    16141 catagcataa tttaagtatt tctacaagca gttaaaactt gactttgtgt ttttgttatt
    16201 ttataatcta gttatcaaaa ctatataaaa aggagttgaa aatgaaaact accttttcct
    16261 acccaaaatg ggcagaaatt ccaaacattg acctctatct ggaccaggtt ttgctctatg
    16321 tcaatcaggt ctgcgcccct atctctccta ataaagacaa gggcctaaca gcatctatgg
    16381 tcaacaatta tgtgaaaaat ggttacctga caaagcctga caaaaaaaaa taccaacgcc
    16441 aacagattgc ccgtttgatt gctatcacaa ccctcaagtc tgtattttct attcaagaaa
    16501 tagcccagac acttaatact ctacaaactc aagcaagttc agaccaactc tacgatgctt
    16561 ttgtggacta catgaaccaa ggaattgacc cagctaaccc tattatccaa accagctgcc
    16621 aaaccgtcaa actctatcat caaactctag acttaatcga tcatactcaa gaggaggtaa
    16681 tccaatgaac actagtctta aactcagcaa acaactcagt tttggagagg agattgctaa
    16741 tagcgtgacc catgctgtgg gtgcagtcat catgcttatc ttgctgccta tttcatccat
    16801 ctatagttat gaagcacacg gatttttatc ctctatcggc gtttccattt tcgtcatcag
    16861 tctctttctc atgttcctat catccaccat ttatcactct atggcctatg gttcgaccca
    16921 caaatatgtt ttgcgaatca ttgaccattc tatgatttac gttgccattg ccggctcata
    16981 cacgcccgtt gtcttgacct tgatgaataa ctggtttggc tatctgatta ttgtcatcca
    17041 atggggaacg accatctttg gtattctcta taaaatcttt gctaaaaagg tcaatgagaa
    17101 atttagcctt gctctttacc tgattatggg ctggttggtt ctggctatca ttcctgccat
    17161 tatcagtcaa acgacacccg ttttctggag tctcatggta actggcggac tctgttatac
    17221 agttggagct ggattttatg ccaagaaaaa accttatttc cacatgattt ggcatctctt
    17281 tatcctagct gcgtccgcac ttcaatacat cgctattgtt tattacatgt aaaaaagttg
    17341 agaaattcaa tctcaacttt tttctttaca catattgata aagtactggt gcaagcgcac
    17401 atcatcagtc aattctggat gaaaagaact taccaacata tttttttctt gggctgcaac
    17461 aatttgattg ttcactgttg ctagaatttc tacaccctca ccaacactac tgataatcgg
    17521 accacggata aaggtcattg gaatcttgcc aactccctta cattctgctt ccgtgtagaa
    17581 acttcctaat tggcgcccat aagcattacg ctcgaccacc atatccatag ttcctagatg
    17641 actctctttc tgagaagtga tttccttagc cagcaaaatt aagcccgcac aggtcccaaa
    17701 cactggtaag ccagatagaa tggcttctcg gatgggaagt agcatgttct ggtcacgtaa
    17761 gagcttgccc atggttgtag actcaccacc aggcaaaatc aaacccgaca agtcactctg
    17821 atcttgctga aaatcatcta gatttctgag ttctacactc tcgacaccta attgatctag
    17881 cacttttgca tgttctgcaa aggccccttg caaggccaat attccaattt tcatctattt
    17941 tcctcgttca gccatgagaa tttggatttc attttcatta ataccaacca tggcttctcc
    18001 taaatcttca gagatttgag ctaggatttg aggattacgg aagttagtca cagccttaac
    18061 aatggcactc gctcgtttaa caggatctcc tgacttgaaa atacctgaac cgacaaagac
    18121 cccctctgcc cctaattgca tcattaacgc agcatctgct ggcgttgcaa cacctccagc
    18181 agcgaaattt acaactggca attttccatg ttcatgaaca tattggacca attctacagg
    18241 gacttgcaaa tccttggcag caacataaag ctcgtcctca cgtaagtttt gaatgcggcg
    18301 aatttcctga ttcatcatac gcatatgacg aacagcttgg acgatatccc ctgtccctgg
    18361 ttctcctttg gtacgaatca tggaagcacc ttcagcgata cgacgcaagg cttcacccaa
    18421 atccttagca ccacagacaa aaggaacttg gaattctttc ttgtccacat ggaaacggtc
    18481 atcagctgga gatagaactt cactctcgtc gatataatca atttcaatag cctctaaaat
    18541 ctgagcttca acaaaatgcc cgattctgac cttagccatt actggaatac taaccgcttc
    18601 ttggatttcc ttaatcatct ttgggtcgct catgcgggaa actcctccag ctgcacgaat
    18661 atcagccgga attcgttcca aggccatcac agctgccgca ccagcagctt ctgcgatacg
    18721 agcctgttca ggattctgca catccataat aacaccaccc ttgagcatct gtgccaagtt
    18781 tttatttagt tcataacgat tttcagtcat ttgttttatc ctcatcattt gtattggtag
    18841 ctaccttttc attttaagtc agaacagaat gaagcacaag ataaaaaaac tataattgta
    18901 tggggacaga ttggctaaaa actatctgac cttaacttaa ggccagatag ctcattcatt
    18961 tttatttttc agccgtaagg gcagccattg tgatgtagtt gtatggttta ttgaagtgtg
    19021 gcaagaagaa gaggtctgtc aatgccaatt tatcaattgt cacatgctct tggatagcaa
    19081 gtgagaacat gtggattccc atgctaattg caatatcatg tgaaaccatt tgggcaccaa
    19141 gaatttcacg gctatctttg tcaaagacaa tcttaattgc tacttcatgg ttgtcatgtt
    19201 tcatgaattc tggtttttga agatcgttaa agcctgtttc agttgcgttg taaccagcag
    19261 ctttcgcttt ttcaagagtc aaaccagttg aaaccatgtg aagaccgtag attgagatac
    19321 catttgaacc ttgaacaccg attccttcca attcatgtcc acaagcgttg taggcaccaa
    19381 cgataccagt gcgaacagca tttgaagcaa gagcgatata gcttgtatct ttacgagcat
    19441 tgtcataaac agtcgcacag tcaccaacag cgtaaacgcc tgggatagat gtttcttgtt
    19501 tcttgtctac aaggaaggca ccgttgcgga agagttcgat cttaccatca gcaagggctg
    19561 tgtttggacg gaaaccaact gcaaggataa ccatatccac gtcaaagctt tctttgtcag
    19621 taatcaagcg ttcaacttta ccatcacctt cgattgcttt aacagtttga cctagagcca
    19681 agcggatgtt gtgatcttcc aagttcttcg ccatcatttg tgtgaagtct ttgtcatagt
    19741 aaccgttcaa gacagtatca acgatatcaa caaggacaac ttcttttcca agacgttcaa
    19801 aggcttcagc aagttcaaca ccgatgtaac caccaccaac aacggcgata cggtcgagat
    19861 gttggctctt gtcagaaagt ttattgataa cttcttcagc attttggtac aatttcacga
    19921 attgtacgtt ttcaagagtt gctttaaatt cgcggtttcc tttaacaatt tcaacacctt
    19981 cgattggtgg caagattggt gtagaacctg tagcgaaaat caatttctcg tatgattctt
    20041 tgtgttcttt tccttcaact tctgctgtaa ccactttgtt atcatagtcg attgaaagaa
    20101 ctggtgaatt catataaacc ttagcacctt tagcttctaa tttgtcttta tcagaataga
    20161 acaagccttc agcaccgtca atttgttcac caatccaaag agccattcca catcctagga
    20221 aagagatgtt agagttttgg tcaaatacaa caatttcgtt ctcatttcca aaattatcca
    20281 acatggtatt gatacatgct gtaccagcgt ggttagcacc gactacaacg attttactca
    20341 taaaaaattt cctgccttca ttctaaattt actttactag tataccattt accgttagcg
    20401 tttacaaggg ggggactatt atttcagcat tttttatcaa aaataattcc ccatttatcc
    20461 tgttttcaag cctcatgaat tttatgagca tatttcttga ctttttccaa attttgtttg
    20521 tgaaaattct aactttatgg tattataata acatgaaaat aaaatgtaag ggcttcaatt
    20581 tagatgatta tttgatattg caacttattt tctataagag aaaggagttg gtagcttgac
    20641 tcttcgttca cattctgaac ggctaatggg gactactatc actatttcat tagtggatga
    20701 gcaagccgat atctttctcc aaaaatcctt tgacttgctc aaagagcttg aataccgctt
    20761 caacgccaat agtcaagaat ctgagttgat ggaaatcaat tatcaagcag gaatatcccc
    20821 cgtcacagtt catccagacc tgtttgagct gatttcactt gggttagagc atagcctagc
    20881 gctctctagc cacctcaata tcagcattgg tcccttaatt caaacctggc gtatcggttt
    20941 ttcagatgcc aaggtcgccc agcctcaaga aattgaatcg gtgctgcctc taatcaatcc
    21001 tcatggtatc gagttagatt cttctacttc tactgtgttt ttaaaacaga aaggaatgaa
    21061 gatcgatcta ggttgtttag ccaaaggata cagtgcggat aaggttgccc aatttcttag
    21121 gaaagagggg gtgacttctg ccttgatcaa tctgggaggg aatatcctga ccattggaaa
    21181 aaatcaggca agaggggata acccatggca aatcgggatt caagacccag ccaatcctag
    21241 gggaaatcat ttaatgacca tccctgttgt caataaatct gtcgtgactt caggcattta
    21301 tgaacgtcac ctgaccgtcg atggacaaga ttaccatcac atttttgaca gtcaaacagg
    21361 atatcctgtt gaaacggaac tagcgagtct aacaatcatc tctgataaat cagtcgatgg
    21421 cgaaatctgg acgactcgac tctttggaga aagacctgct tctatcctct ggcaagtcga
    21481 aagtttggag ggcatcgaag tcatcctcat cgataaagaa ggccacctaa gctgttcttc
    21541 aggaattcct actctataga gacaactgtt tcaatggaat tttaaaattg tattatgtaa
    21601 aaagagaaag gaaatatcat gttaaaactt attgctattg ttggaacaaa ttcaaaacgt
    21661 tctacaaacc gtcaattgct tcaatacatg caaaaacact ttgctgacaa agctgaaatt
    21721 gaacttgttg aaatcaagac cattcctgtc ttcaacaaac cagctgacaa gcaagtacct
    21781 gctgaaatat tggaaattgc tgctaaaatc gaagaggcag atggcgttat tatcggtact
    21841 cctgagtatg atcactctat tccagctgtt ttgatgagcg ctcttgcttg gttgtcttat
    21901 ggtatttacc cacttttgaa caaaccaatc atgattacag gtgcttctta cggtacgctt
    21961 ggttcatctc gtgcccaatt gcaacttcgt caaatcttga atgctcctga aatcaaggca
    22021 aatgttcttc cagatgaatt cttgctcaca cactctcttc aagcatttaa cccaagtggc
    22081 gacttggttg accttgatgt tatcaagaaa ttggatgcca tctttgatga cttccgtatc
    22141 ttcgtgaaga ttactgagaa attgcgcaat gcgcaagaat tgcttcgcaa agatgctgaa
    22201 gactttgact gggaaaattt gtaagatagg agaccgaaag aatgaaattt gttggacttg
    22261 ttggatcaaa ctacgatcaa tcatataacc gcaaactctt ggaatttatc cgtcgcaatt
    22321 tcaaattcaa atttgaatta gaagtacttg aaatcgacga agttccaatg tttaaccaag
    22381 acgaaaaatg ggacgaaagc ttccaattgc gtttcttgta taacaagatt acacgtgctg
    22441 atggtgtcat tatcgccact cctgagcaca accacactat ctcagcttca ctcaaatctg
    22501 tacttgaatg gctttcatac gaagttcatc catttgaaaa caagcctgtt atgattgtgg
    22561 gtgcatcata ctatgaccaa ggaacatcac gtgctcaagt tcaccttcgt aaaatccttg
    22621 atgctccagg tgttaatgcc tacacgcttc caggtaacga attccttctt ggtaaagcta
    22681 aggaagcttt tgataacaac ggaaatatca ccaacgaagg cactgttaaa ttccttgaaa
    22741 cttgcttaga taactttgtt aaatacgtag gagtcgtttc gaaattgaaa aaaccaaaac
    22801 caatcgaatc agaagacttg gattgtggaa aaccaatcgc tacaaccatc acagaagttg
    22861 accctgacga tccagaatgg gtagaaaaag ttgcagcaat cactggtgct gtttctggtg
    22921 atacctatgt caaattggac cacggtatcc taacagttaa ccaaattgat atgttcttga
    22981 aagctatgcc atttgaattg acatacgctg acgacaacaa ccaattcctc tactacaaca
    23041 acgctcacca agatccagac actatgttcg ctaaacgtgt accacctcaa tcaggtagcc
    23101 gtatgtcgac tgttcatggt tctcttccac cagcacgtat gaagaacgta gagtgggtta
    23161 tcggaacact tcgcaacgga aaccaagaat acgtccgtac gatcgttcca ggttctcctg
    23221 caggtgttat caacactcac aactaccaag caatgtacta tcctgatgga tcatacgctg
    23281 gtatcaatga aatcgtcttt aacttccaac catggcttga ctggtaccta aaagagactg
    23341 gtcaacgttt ggtgggtggt agcggaccat ttgctcctgc tgccggaggt catggagacg
    23401 ccgatgctac ttctggcgct tctgattcag gggatgctgg aggccacggt ggtgacgcag
    23461 acgctacagc cggtgcaagt ta
//
LOCUS       DAVBBS010000032        23312 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVBBS010000032 DAVBBS010000000
VERSION     DAVBBS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23312
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.032"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(196..654)
                     /locus_tag="VQU32_001229"
     CDS             complement(196..654)
                     /locus_tag="VQU32_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HET2472686.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(651..1091)
                     /locus_tag="VQU32_001230"
     CDS             complement(651..1091)
                     /locus_tag="VQU32_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2472687.1"
                     /translation="MKVELQISETYEEEKLIVQAPQPTDKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            1675..2541
                     /locus_tag="VQU32_001231"
     CDS             1675..2541
                     /locus_tag="VQU32_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2472688.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLHALAKKLKVSMDYFFDEQVVEKVEELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKIYMEWIKSLIDFYFYGQKEDAIVRLEGVLSQLSVSDLNYLQVSNTLFN
                     FYYDIGDLGRFNKIREKLEYQVNHLNLSTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKAYRTNYLLADLYLLMGNVSENFSSKSSVKEYFETAYFLYKLEENMSMT
                     LKVEHYIADITE"
     gene            2878..3435
                     /locus_tag="VQU32_001232"
     CDS             2878..3435
                     /locus_tag="VQU32_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase family
                     flavoprotein"
                     /protein_id="HET2472689.1"
                     /translation="MDKKNLLVLVTGSVGASNVDTYLYYIKKFYNVKVILSENSKKFI
                     SKELISYFCDKVYDEIFVEEVVPHVFLPYESDLLLILPATANVIGKIANGIADDLVTA
                     TVLNFNKKIIFCPNMNSTMWDNHIVQRNVSILKELGHIFLFESKKTYEVGLRKAIDST
                     CSMLQPQSLVKELIKLENIVLEEGH"
     gene            3656..4762
                     /locus_tag="VQU32_001233"
     CDS             3656..4762
                     /locus_tag="VQU32_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET2472690.1"
                     /translation="MINKKIQQVVLESLQNFLNGNFISPCVVYDFGLLETVLDEFKNQ
                     IPVTFNYQLFYAVKANSNEKILEFLVDKIDGVDVASLSELDVAKKFFPPTQISVNGPA
                     FSYETLYNLIKKQYKVDINFLEHLQQFSPKESVGIRVTEPDELNNRMSRFGINICSDN
                     WTSNLQNPLITRLHFHFGEKDDKFIVKLDKILFKLQEINKLREVREINLGGGFMKLFM
                     ENRLKEFFLSLMEIYKKYDIDSTVTTIIEPGSAITSFSAYMITSPVNVSEVNEQQVIT
                     LDTSIYTNTLWFVPHIITTLNSSSKERYSTILYGNTCYEHDKYKMKVSLPRLTQNSSI
                     VFFPVGAYIKSNHSNLHRNDFMREVYLWTKNLTY"
     gene            4738..6129
                     /locus_tag="VQU32_001234"
     CDS             4738..6129
                     /locus_tag="VQU32_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472691.1"
                     /translation="MDKKLDILDKVKEYLGNKTTQILDNQYKEFLKLNDIRRAFGISE
                     KVLNNSFNFTSKEFNDLINNENYLFEYACRIREEWRKKCFNHSYRFLCSPIITDDFLN
                     TKTLRSSQIEYKYERYLSKSSIGDRAVDGFVSFNTLTANGMSAIKLCLEILNSIFFKK
                     KIDLLYSTGYYETRFLLNNLAKSGISCYEVSNCELDKDKFYNVFMMEPNRADLTLQKT
                     DFKIVEYFVKYKNNSIKVVILDISYQGSNFKLVEFLEKFKFANVIIFVVRSLIKLDQM
                     GLELTNGGIIEVFIPNHLRKLKNFIEEEFNKFRNSHGANLSLYEYCLLDNSLTLKNDW
                     NYSDLVMKFTSNFYADIKDLFMENSDIEIIHEEGVPFVFLDLIGEGKKEYEMFFQWLN
                     FFYKQLGITLYARNSFGFRNLTVEYFGIIGTERYIFKICPGVYKGLSYYLMKFLLKSF
                     SNEYLKTTDEVNR"
     gene            6126..7304
                     /locus_tag="VQU32_001235"
     CDS             6126..7304
                     /locus_tag="VQU32_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2472692.1"
                     /translation="MKNLIKLLIIRLIVNLADSVFYIVALWHVSNNYSSSMFLGIFIA
                     VNYLPDLLLIFFGPVIDRVNPQKILIISILVQLAVAVIFLLLLNQISFWVIMSLVFIS
                     VMASSISYVIEDVLIPQVVEYDKIVFANSLFSISYKVLDSIFNSFASFLQVAVGFILL
                     VKIDIGIFLLALFILLLLKFRTSNANIENFSFKYYKREVLQGTKFILNNKLLFKTSIS
                     LTLINFFYSFQTVVVPIFSIRYFDGPIFYGIFLTIAGLGGILGNMLAPIVIKYLKSNQ
                     IVGVFLFLNGSSWLVAIVIKDYTLSLILFFVCFMSKGVFNIIFNSLYQQIPPHQLLGR
                     VNTTIDSIISFGMPIGSLVAGTLIDLNIELVLIAISIPYFLFSYIFYTDNGLKEFSIY
                     "
     gene            7729..7962
                     /locus_tag="VQU32_001236"
     CDS             7729..7962
                     /locus_tag="VQU32_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2472693.1"
                     /translation="MISIDLGGTNIKITVLSNDGEIETLWSITTDTSEKGSQIISDII
                     SSIKNKLTERNIPDSDLLGIGMGSCSSYFPCKS"
     gene            8245..10194
                     /gene="mutL"
                     /locus_tag="VQU32_001237"
     CDS             8245..10194
                     /gene="mutL"
                     /locus_tag="VQU32_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET2472694.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKIQITDNGYGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(10519..10986)
                     /locus_tag="VQU32_001238"
     CDS             complement(10519..10986)
                     /locus_tag="VQU32_001238"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET2472695.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(10987..12192)
                     /locus_tag="VQU32_001239"
     CDS             complement(10987..12192)
                     /locus_tag="VQU32_001239"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET2472696.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(12212..12847)
                     /gene="ribE"
                     /locus_tag="VQU32_001240"
     CDS             complement(12212..12847)
                     /gene="ribE"
                     /locus_tag="VQU32_001240"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET2472697.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(12832..13932)
                     /gene="ribD"
                     /locus_tag="VQU32_001241"
     CDS             complement(12832..13932)
                     /gene="ribD"
                     /locus_tag="VQU32_001241"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET2472698.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(14067..14181)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            14338..14931
                     /gene="ruvA"
                     /locus_tag="VQU32_001242"
     CDS             14338..14931
                     /gene="ruvA"
                     /locus_tag="VQU32_001242"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET2472699.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            14941..15504
                     /locus_tag="VQU32_001243"
     CDS             14941..15504
                     /locus_tag="VQU32_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET2472700.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRDFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            15501..16178
                     /locus_tag="VQU32_001244"
     CDS             15501..16178
                     /locus_tag="VQU32_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2472701.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     VGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(16319..17350)
                     /locus_tag="VQU32_001245"
     CDS             complement(16319..17350)
                     /locus_tag="VQU32_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HET2472702.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGRALFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNAGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(17605..18288)
                     /locus_tag="VQU32_001246"
     CDS             complement(17605..18288)
                     /locus_tag="VQU32_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HET2472703.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASVFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(18300..19244)
                     /locus_tag="VQU32_001247"
     CDS             complement(18300..19244)
                     /locus_tag="VQU32_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET2472704.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            19376..22207
                     /gene="uvrA"
                     /locus_tag="VQU32_001248"
     CDS             19376..22207
                     /gene="uvrA"
                     /locus_tag="VQU32_001248"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET2472705.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAIKASSVEQIVDKVLELPERQRLQILSPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDNSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPNASKTLHEGALAPWNPISSNYYPNMLEQAMTAFGVAMDKP
                     FEDLSEEDKNLILYGSDGKKFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCHGYRLNDQALSVRVGGEQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            22200..23261
                     /locus_tag="VQU32_001249"
     CDS             22200..23261
                     /locus_tag="VQU32_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET2472706.1"
                     /translation="MNKRVQAFLAKMQEKELDGVIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
BASE COUNT         7035 a         4105 c         4754 g         7418 t
ORIGIN      
        1 agatctgaaa taactgttat gatatagagg attgaaacta gaatagtaca cctctgcttc
       61 taaaacattg ttagaaatcg atttgactgt cctgatcgat ttatcctgtt cttatttcat
      121 ttcactatag taaggtgcag caggataaag aaaaaccgag aagtttcttt ctcggatttg
      181 cttattatga ggttcctatt ttctatgctc caagagttga ttgatgtgat ctactttttt
      241 agcctctctt ttatagagaa tagcccagat gatgagatag acaatcgcaa actcgataat
      301 gagctggagg tagaaaatcc agtggaaagg gaaccaacca gcaagagttg ctagtgggac
      361 aaagccagcc agcataagga agaaatgagt cagagtggca cgaagcatgc tccagtcacg
      421 gctgaataag cgattgccaa agttgaagag cataccaatg gctgcccaga taagtgtgca
      481 gtagagcaag accaaggcac cgtgaacctg atgctgatcc atcacttggc cgatgagaga
      541 gtagggattt agtggtgcgt aggtatttgg tgcataaatg agtgaaaaga ggatagagag
      601 gatgaggccg ataagaacac cggcagctgc atcgtgaaat acttgttttt tcatagttct
      661 aatttctcct tgatggtttt tagataacgg cgtgaagagt aggtgaagct ttcgtttttc
      721 aagaaaattt ctaccagacc gtttggcgtg agcttgaggt gagagatgga atcgatattg
      781 atgatttctg attgggaaat ttggataaaa ttggttggca agagtttaag aacctgatag
      841 agtcgcaaat caatgctgta ggtctgactc gcggtttctg ctagaacctt ccgattctcg
      901 atatagaagc gctgaatctt gccaatctca actagataga cctgatcatc gattttccct
      961 ttgatttttt ctctttggtc cagattttct gcgaactcga tgactttctg aactttatct
     1021 gtcggctgag gtgcttggac aatcagcttt tcctcctcgt aagtctcact aatctgtaat
     1081 tctactttca taaatttctc tccttgtttt tcatacaaaa ttaatctcac ttcttgtaca
     1141 cctttattaa tactcttcaa aaatctcttc aaaccacgtc agcgtcgtct tgccgtagga
     1201 tagttgttga ctttgtcagt tttatctaca acctcaaaac agtgttttga gctgtctgcg
     1261 cctagctttc tagtttgctc tttgattttt attgagtata ccactatttt actccctctg
     1321 gcaaggtact ttgtctatgt ggagggattg ggctcctatg tggtggagct tttctgttct
     1381 ttctgaaata tggtataata gcactaatca atttctagga aaatagatac agaaaggggc
     1441 tgaaagaact aggggaacag ttgtaattag aaggtgatag gtatgaaaag tggcgttgaa
     1501 gctggataag tttataaaga gtttgaatga agaaatgaaa agtaaataag ttgatgatga
     1561 attgcaaaaa tagattttat atacaatttg tatctttcaa atcattgatt tgaattttag
     1621 atttaatcat gttacaatta aaaatgaaat caaatagaag aggaagatgc aaacgtgggc
     1681 acactattag caacaagatt aaaaaataga cgaaaagaat taaaaatgtc tcagcgagaa
     1741 ctagctgaag gtatctgtaa acaggggcaa atcagtcgat tagagagcgg agagtttact
     1801 cctggagcag atttcttgca tgcccttgct aagaagttaa aagtcagtat ggattatttt
     1861 tttgatgagc aggttgtaga gaaagttgag gagttatcag aatttaagaa attagcacag
     1921 acatttatta caaatcgcaa ttatgagtct ttgaaatata tatatgaatt ggagaatgta
     1981 aaagctcacc gcctatcttt agcagataaa atctacatgg aatggataaa atctctgata
     2041 gatttttatt tttacggaca gaaagaagat gcgattgtac gattagaggg ggtattatca
     2101 cagttaagtg tatctgattt gaactatctc caagtttcaa acaccctatt caatttttat
     2161 tatgatattg gagatttagg tcgtttcaat aaaattcgtg aaaaactaga gtatcaagta
     2221 aatcacctca atttgagtac aatcgaagag ttagaactat ctattaagtt taactacaac
     2281 gtttgtcgtt atctatggtt acagaaaaat attgaagaag ctattactaa aattacagct
     2341 actataaaac aatgtaaggc ttacagaacg aattatcttt tggctgattt gtacttattg
     2401 atgggaaatg taagtgaaaa tttttcttct aaaagttcgg tgaaagaata ttttgagacg
     2461 gcatattttc tatacaaact tgaggagaac atgtcaatga ctctgaaagt tgagcattac
     2521 attgcagata taacagaata atgaatacgg tgttcaagtg agcatcgttt ttcaaaccta
     2581 gttcagatac aaaaaagtat caaataaaaa aaaataaaaa aacattataa aaacaattga
     2641 caagctaatt ttgaagtgat aaaataaatg caagaaaaca aaaggagagg tatatgagaa
     2701 gtttgtttaa gaaaatagtt gctgtattag ttattggctt aattctgtta ggaattgcga
     2761 gaacacctca agttcataag atggctagag ggattgatcc tggtcctgcg aattttattt
     2821 agaaatcaat ttctactaat tgagtactaa ttgagtatta atgggtaaaa agattgtatg
     2881 gataagaaaa atttacttgt attggttact ggttcggtag gtgccagtaa tgtagataca
     2941 tacttatatt atataaaaaa attctataat gttaaagtga tactttctga aaattctaaa
     3001 aaatttataa gtaaggaatt aattagttat ttttgtgata aagtttatga tgaaatattt
     3061 gttgaagagg tagttccgca cgtttttctg ccatatgaat ctgacttact tcttatttta
     3121 ccagctacgg caaatgtgat tggcaaaatt gctaatggta ttgctgatga tttagttaca
     3181 gcaactgttt taaactttaa taaaaaaata attttttgtc ccaatatgaa ctctactatg
     3241 tgggacaatc acatagttca aagaaatgta tcaattctaa aggagttggg acatatattt
     3301 ttatttgagt ctaaaaaaac atatgaggta ggattgcgta aagcaataga ttcaacatgt
     3361 tcaatgttac aaccacagtc gttagtaaaa gaacttatca aattagaaaa tattgtcctt
     3421 gaagagggac attaaaaact actgagaata ttaaggaggg gaaaaaatgg aaaattcatc
     3481 aatcgatgta gatatgctgt tggaagaatt gacacaagaa gcaatggtcg ttgttgctgt
     3541 tgataaggac tgttaattta aacttatggc aatatatgaa aggttactgg atgttttaaa
     3601 ttatgcaggc agtagccttt tattatatac aaatggataa agtaaggata atacaatgat
     3661 taataaaaaa atacaacaag ttgttttgga atcattacag aattttttga atgggaactt
     3721 catttcgcct tgtgtagtct atgattttgg cttgctggaa actgtacttg atgaatttaa
     3781 aaatcaaatt cctgtaacat tcaattacca acttttttat gccgttaaag caaattcaaa
     3841 tgagaagata cttgaattct tagtagataa aattgatgga gttgatgtgg cgtcattatc
     3901 tgaattagat gtggctaaaa aatttttccc accaactcaa atttctgtta atggtcccgc
     3961 attttcttat gaaactttat ataatctgat taaaaaacaa tataaagttg atattaactt
     4021 tttggaacat cttcaacaat tttccccaaa agaatctgtt ggaataagag taacggagcc
     4081 agatgaactt aataatcgta tgagtcgatt tggaataaat atttgcagtg ataattggac
     4141 tagtaattta caaaatcctt taattacacg actgcatttt cattttggag aaaaagatga
     4201 taaatttatt gttaagttag ataaaatatt atttaagtta caagaaatta ataaacttag
     4261 agaggttaga gaaataaatc ttggaggcgg ttttatgaaa ttatttatgg aaaatcgttt
     4321 gaaagaattt tttctatcac ttatggaaat ctataaaaag tacgatattg atagtactgt
     4381 gactacaata atagaaccag gtagtgcaat tacttcattt tctgcctata tgattactag
     4441 cccagttaat gttagtgagg tgaatgagca gcaggttatc acgttagaca catcaatata
     4501 caccaataca ttatggtttg ttccgcatat tattacaacg ttaaattcaa gtagtaaaga
     4561 gcgttatagt actattctct atggtaatac ctgttatgaa catgacaagt ataaaatgaa
     4621 agtttcgctt ccaaggttaa ctcaaaatag cagtatagtg ttttttcctg taggagctta
     4681 tataaaaagc aatcattcaa atttacatcg taatgatttt atgcgggagg tatatttgtg
     4741 gacaaaaaac ttgacatatt agataaagtt aaggaatatt taggaaataa aactactcaa
     4801 attctggata atcaatataa agaatttttg aaacttaatg atataaggcg agcgtttggt
     4861 atttcagaaa aagtattaaa caattctttt aattttacga gtaaagaatt taatgattta
     4921 attaataacg aaaattattt attcgaatat gcatgtagaa ttagagagga atggagaaaa
     4981 aaatgcttta atcattctta tcgttttcta tgctcaccta taattacaga tgattttctt
     5041 aacacgaaga cattgagaag tagccaaatt gaatataaat atgagcgata tttatcgaaa
     5101 agttcgatag gcgatagagc ggttgatggc tttgtttcct tcaatacttt aacagctaat
     5161 ggtatgtctg ctattaaact atgtcttgag atattaaact ctattttctt caagaagaag
     5221 attgatttat tatattcaac cggatattat gaaacaagat ttttattaaa taatcttgct
     5281 aaatcaggta ttagttgcta tgaggtaagt aattgtgaat tggataaaga taaattttat
     5341 aatgtattca tgatggaacc caatcgagcc gatttaacat tacaaaaaac tgatttcaag
     5401 atagtagaat attttgttaa gtataaaaat aattcaataa aagtcgttat tttagatatt
     5461 tcatatcaag gttctaattt taaattagta gaatttttag agaaatttaa atttgcgaat
     5521 gtaattattt ttgtggtacg atctttgata aaattagatc aaatgggatt agaattgaca
     5581 aatgggggaa taatagaagt gtttattcct aatcatttga gaaagttgaa aaattttatt
     5641 gaagaggaat tcaataaatt tagaaattct cacggagcta atctaagcct ctatgaatac
     5701 tgtttgcttg ataattcttt aaccttaaaa aatgattgga actattctga tttagttatg
     5761 aaatttacga gtaattttta tgctgatata aaagacttgt tcatggaaaa ttctgatatt
     5821 gaaatcatcc atgaagaggg agtacctttt gtatttttag atttaatagg tgaaggtaaa
     5881 aaagaatatg aaatgttttt tcaatggtta aacttctttt acaaacagct tggaatcaca
     5941 ttgtatgcta gaaatagttt tgggtttcgg aatctaacag tagagtattt tggaattatt
     6001 gggacagaaa gatatatatt taagatttgt ccaggtgttt ataaagggtt aagttattat
     6061 ttgatgaaat ttttattaaa atctttttca aatgaatatt taaaaactac tgatgaggtt
     6121 aatagatgaa aaatttgata aagttgctaa taattagatt gattgttaac ttagcagaca
     6181 gtgtatttta tatagtagca ttgtggcacg ttagcaataa ttattcttcg agcatgttct
     6241 taggaatatt tattgcagta aattatctac cggatttgtt actaatcttt tttggaccag
     6301 ttattgacag agtaaatccg caaaaaattc ttataatatc aattttggtt caattagcag
     6361 tggctgtaat atttttatta ttattaaacc aaatatcatt ttgggtgata atgagtctag
     6421 tgtttatttc agtaatggct agctccataa gttacgtgat agaagatgtg ttgattcctc
     6481 aagtggtaga atatgataag attgtatttg caaattctct ttttagtatt tcgtataaag
     6541 tattagattc tatttttaat tcattcgcat catttttaca ggtggcagta ggatttattt
     6601 tattggttaa gatagatata ggcatatttt tacttgctct atttatattg ttgttgttaa
     6661 aatttagaac tagcaatgcg aatatagaaa acttctcttt caaatattac aagagagaag
     6721 tgttgcaagg tacaaagttt attttaaata ataaattatt atttaaaacc agtatttctt
     6781 taacgcttat aaactttttt tattcatttc agacagtagt tgtaccgatt ttttctattc
     6841 gatattttga tggtccgatt ttttatggta tttttttaac tattgctggt ttgggtggta
     6901 tattgggaaa tatgctagcg ccaatcgtaa taaaatattt aaaatcgaat caaattgttg
     6961 gtgtatttct ttttttgaac ggctcaagtt ggttagtagc aattgttata aaagactata
     7021 ctttatcact tattttattt ttcgtttgtt ttatgtctaa aggagtcttc aatattattt
     7081 ttaattcgtt gtaccaacaa atacctccac atcaacttct tggtagggta aatactacca
     7141 ttgattctat tatttctttt ggaatgccaa ttggtagttt agttgcagga acgcttattg
     7201 atttgaatat tgaattagtg ttaattgcta ttagcatacc ttattttttg ttttcttata
     7261 ttttttatac ggataatgga ttgaaagaat ttagtatata ttagaaatgt ttatgttcat
     7321 tcaaaagcat aatgactata actgaaaaag aaaagtgata tctttaaggt tgttcttctt
     7381 ggtggtgaga ttcgtgagac aacccaagct tttgtcggaa agattaccaa tgctttgatg
     7441 gataggatgt actttagcaa gatgttttta gtggtaacgg tatcgtggat ggacgtgtaa
     7501 taacctcttc tttcgaggag tattttacta aaaaactagc cttggagcgt tccccagaaa
     7561 cggacttact cattgactct tcaaagattt ggggagaaga ttttgcttca tctgttcctt
     7621 gaaaaaagtc acagcagtca tcacagacga tagtactgaa caaaactatg aagagttaga
     7681 aatttatacg caggtgattg tataaaggat ctggaaatag ataagaagtt gattagtatt
     7741 gacctaggtg gtacaaatat taagattact gttctttcaa atgacggtga gattgaaact
     7801 ttgtggagta ttacaacaga tacaagtgag aaaggttctc aaattatatc ggacatcatc
     7861 agttctatta aaaataaatt gaccgaacgg aatattcctg atagcgacct tcttggaatc
     7921 ggtatgggaa gttgctcatc atactttcct tgtaaatcat aggggctata aactctccgt
     7981 ctacttgtcc tgcaacaatt gaagtctgct caaaacgccg tccgctaatc ttttcataga
     8041 ctttctccct tttaggagcc tagctttcta gtttgttctt tgatttttat tgagtatacc
     8101 actattttac tccctctggc aagggacttt gtctatgtgg agggattggg ctcctatgtg
     8161 gtggagcttt tctgttcttt ctgaaatatg gtataatagc actaatcaat ttctaggaaa
     8221 atagatacgg aaaggggctg aaagatgtct catattattg aattgccaga gatgctggca
     8281 aaccaaatcg cagctggaga ggtcattgaa cgtcctgcca gcgtggtcaa agaattggta
     8341 gaaaatgcca ttgacgcggg ctctagtcag attatcattg agattgagga agctggtctc
     8401 aagaaaatcc aaatcacaga taacggttat ggaattgccc acgatgaggt ggagttggcc
     8461 ctgcgtcgcc atgcgaccag taagataaaa aatcaagcag atctctttcg gattcggacg
     8521 cttggttttc gtggtgaagc cttgccttct attgcgtctg ttagtgtctt gactctgtta
     8581 acggcggtgg atggtgctag tcatggaacc aagttagtcg cgcgtggggg tgaagttgag
     8641 gaagtcatcc cagcgactag tcctgtggga accaaggttt gtgtggagga tctctttttc
     8701 aacacgcctg cccgtctcaa gtatatgaag agccagcaag cggagttgtc tcatatcatt
     8761 gatattgtca accgtctggg cttggcccat cctgagattt cttttagctt gattagtgat
     8821 ggcaaggaaa tgacgcggac agcagggact ggtcaattgc gccaagcaat cgcagggatt
     8881 tacggtttgg tcagtgccaa gaagatgatt gaaattgaga actctgacct agatttcgaa
     8941 atttcaggtt ttgtgtcctt gcctgagttg actcgggcta accgcaatta tatcagcctc
     9001 ttcatcaatg gtcgttatat caagaacttc ctgctcaatc gtgctatttt ggatggtttc
     9061 ggaagcaagc ttatggttgg acgttttcca ctggctgtca ttcacatcca tatcgaccct
     9121 tatctagcgg atgtcaatgt gcatccaact aagcaagagg tgcggatttc caaggaaaaa
     9181 gaactgatga ccctggtttc agaagctatt gcaaatagtc tcaaggaaca aaccttgatt
     9241 ccagatgcct tggaaaatct tgccaaatcg actgtgcgca atcgtgagaa ggtggagcaa
     9301 actattctcc cactcaaaga aaatacgctc tactatgaga aaactgagcc gtcaagacct
     9361 agtcaaactg aagtagctga ttatcaggta gaattgactg atgaagggca ggatttgacc
     9421 ctgtttgcca aggaaacctt ggaccgattg accaagccag caaaactgca ttttgcagag
     9481 agaaagcctg ctaactacga ccagctagac catccagagt tagatcttgc tagcatcgat
     9541 aaggcttatg acaaactgga gcgagaagaa gcatccagct tcccagagtt ggagtttttc
     9601 ggacaaatgc acgggactta tctctttgcc caagggcgag atggacttta catcatagat
     9661 cagcacgctg ctcaggaacg ggtcaagtac gaggagtacc gtgaaagcat tggcaatgtt
     9721 gaccagagcc agcagcaact cctagtgccc tatatctttg aatttcctgc ggatgatgcc
     9781 ctgcgtctca aggaaagaat gcctctctta gaggaagtgg gcgtctttct agcagagtac
     9841 ggagaaaatc aatttattct acgtgaacat cctatttgga tggcagaaga agagattgaa
     9901 tcaggcatct atgagatgtg cgacatgctc cttttgacca aggaagtttc tatcaagaaa
     9961 taccgagcag agctggctat catgatgtct tgcaagcgat ctatcaaggc caatcatcgt
    10021 attgatgatc attcagctag acaactcctc tatcagcttt ctcaatgtga caatccctat
    10081 aactgtcctc acggacgtcc tgttttggtg cattttacca agtcggatat ggaaaagatg
    10141 ttccgacgta ttcaggaaaa tcacaccagt ctccgtgagt tggggaaata ttaaaagtat
    10201 aaaaaagtct gggaaaaatt ttcaaaatca gaaaaacgca taaaatcagg tgttcaaaaa
    10261 ccttgatttt atgcgtttta tcatggaaat agttacttca tgttttccta attcttttcg
    10321 aaactctttt taaaccacgt cagttttatc agtaatctca aaacagtgtt ttgagctaat
    10381 tttgccagtt ttatctgtaa catcgaagtt gtgtttgacc actctgcgac tggtttccta
    10441 gtttgctcta tgattttcac agagtattaa attgcgattt tgccaagttt ctttattcgt
    10501 ctaaaagtag agtctgttct atgcgtctaa tgtacgaatt aggttgacca tttcaatagc
    10561 tccttgtgca cactcagaac ccttatttcc tgctttggta ccagctcgtt ctatggcttg
    10621 ttcaattgta tctgtcgtta gcacaccaaa cataacagga atttcgctat ttaaactgat
    10681 ttgggcgatt cccttagata cctcgctaca tacataatca taatgacttg tattccctct
    10741 aatgacagct cccaagcaga taattgcatc atatttttta ctttttgcca tttttgatgc
    10801 aatcagtggt atttcaaaag ctcctggaac ccaggctacc tctatatctt tctcgtttac
    10861 attctctctt ttgagattat ctagtgctcc agataataat tttgaagtta taaattcatt
    10921 aaatctcgct acaacaatac ctattttaat attgtttgct actaaattac cttcataagt
    10981 gttcatttat ttttcctcca tatttaaaat gtgacccatt cgatttttct ttgtttctaa
    11041 ataaaaacta tcgtaaggat tggcttctat ttcgattgat attctactgg aaatggtaat
    11101 tccatatttt tctaactgtt caaccttgtc aggattattt gtcagtaaat gaagtgactg
    11161 aagtcccaga tctttaagca tttgtgctcc aatatgatat tctcttaaat caccttcaaa
    11221 gcctaatgca agattggcat caagcgtatc catgccttga tcttgtaaat gataggcttt
    11281 taatttattg ataagtccaa ttcctcgtcc ctcctgtcgc aagtaaagta agacacccga
    11341 accattctca acaatcattt tcatagcttt atcgaattgc tgtccacaat cgcaacgtaa
    11401 agagcctaaa acatctcctg ttaaacattc ggagtggacc cgacataata cattggcttc
    11461 atcctctata tttcccataa taagagcaag atgatgttcc ccatttagtt tatctatata
    11521 gctaattgct ttgaaattac cgtatctagt aggcatattg acagttgaaa ctcgttctac
    11581 cagctgatca tatacttttc tatattcttg taattctttg atggtaatta gtggaatgtt
    11641 gtgtttcttc gagaactgaa ttaaatcatc tgttctcatc attttgccat catgattcat
    11701 tatttcacaa cataggccac actcttttag tccagctaat tttaataaat caacagttgc
    11761 ttctgtgtgt ccatttcttt ctagaacacc accttttttt gcaattaaag gaaacatgtg
    11821 tcctggcctg cgaaaatcag agggtgttat atcttcagct acacacatac gtgcggtcag
    11881 tcctctctcc tcggcagaaa tacctgtggt cgtttcttta taatcaattg aaactgtaaa
    11941 agcagtctta tgattatctg tattgttttc aaccataggt gaaagcatta attgattagc
    12001 taaactttcg ctcataggca tacaaattaa tcctttggca taagtagcca taaaattaac
    12061 attttctgtt gtagctgctt gtgcagaaca aattaagtct ccttcatttt ctctatcctt
    12121 gtcgtctata acaagaacaa gtcgtccctt ctgcaatgct tctaatgctt cttgtatttt
    12181 tcgatattcc attgactgat tatcctttct gctaaaatcc attttgatat aatagttcct
    12241 tagatatttc tgattttgga gagttatcca tcagtttttg cacatattta cctaagatat
    12301 cattttcaag atttactgta ctcccgactt gtttactctt aagaatggtt tgttccaagg
    12361 tatgagggat aacagatact gaaaagttta ctttggagac tttagcgaca gtcagactaa
    12421 tgccgtcaat tgtaatagat cctttttcaa ctattaaatc taaaatttct ttttgtgtgt
    12481 tgatttgata ccatacagca ttatcatctt tttttattga cgagattttt cctgtaccat
    12541 caatgtgtcc tgtaacgacg tgacccccaa gtcgaccgtt gacagataag gctctttcta
    12601 gattcacctc acttccatgt tttaatagag taagagctgt tcgactccat gtttcattca
    12661 ttacatcaac tgtaaaggat tgatgattga aatgagtaac tgtaagacag ataccattta
    12721 ctgctatact atcgcctaaa tggatatccg ttaatatttt tgaggcttta attgatagtt
    12781 tacaattacg agagtctttc tgtattcttt caacttttcc gatttcttca attattcctg
    12841 tgaacatgga taaatcactt cactttctat gagatagtca tttcctattt gagaaaaagc
    12901 ataaggtttc aatctaatag cgtcatttgg caaagaaatg ccttcacctc cgacaggaaa
    12961 cttggcactg cctccaaaaa tttttggtgc aatatatatt ttcagctcat caacaatttg
    13021 ttgttccaaa gcactccaat tcattagact gcccccttct agaactaggc tatcaatctg
    13081 catgtttcct agatgttgca ttaaactcga taagtctata tgattgcctt ttttctttat
    13141 ggaaagtatt tcacagccat gattttgata tagcttcatt ttatttttgt cttcagagga
    13201 agtggcaatg taagttttaa tatcatttgc tgtttttacg attttagagg taagaggagt
    13261 tcgtaaatgt gtatcgcata tgatacggat aggatttttc ccttcctcca atctacatgt
    13321 cagcaaagga tcgtcttgaa taacagtatt gactcccacc ataattgcac taacatggtg
    13381 tcgtaactga tgcacatgct ttcttgcttc ttcttcagta atccatttgg attgatttgt
    13441 tttagtggct atttttccat ccattgacat tgcatatttc ataaaaacat agggtacatg
    13501 ctgggtaata tactttctaa aactttttat taagttaaga cactcatttt ctaaaattcc
    13561 aacagtaact tgaagattat tttcctcaag tatctttact ccttttccag atacaatagg
    13621 attacagtct aggcttccaa tgactactct tgtaatacca ctatcgatta tagcatctat
    13681 acagggaggt gttttcccga agtgacaaca gggttcaagt gttacataaa gcgtcgctcc
    13741 gacaggggat tctctacagt ttttaagagc atttctctca gcatgtgggc caccaaaaaa
    13801 ctcatgataa ccttgtccga tgatgtgatt atcttttaca ataactgcgc cgaccatagg
    13861 attgggattg acgtaaccag cccctttttg tgccagtttt attgctaatt tcatatattt
    13921 tgaatcgctc atctcactac ctccaaaaaa aatatacctt gaatagggga ctactcaagg
    13981 catacaaaag aaaacttatg cgattaacaa aaatactctg aaatgacaag taatcatttc
    14041 agagcacgca aaaagcacaa atatactttt atcttctttc atccagacta tactgtcggc
    14101 tttggaattt caccaaatca tgcctttcgg ctcgtgggct ataccaccgg tagggaatta
    14161 caccctgccc tgaagatagt tattcaatta cagatgatta tagtacttaa ttttgaatat
    14221 gtcaacagat aaataccgat tgtttttgat atactgtatt tgtgataatc gattctcgct
    14281 cctcggataa agaaaatatg atatactaga taaacgaaat aagagagaag gaatactatg
    14341 tacgcatatt taaaaggaat cattaccaaa attactgcca aatacattgt tcttgaaacc
    14401 aatggtattg gttatatcct gcatgtggcc aatccttatg cctattcagg tcaggttaat
    14461 caggaggctc agatttatgt gcatcaggtt gtgcgtgagg acgcccattt gctttatgga
    14521 tttcgctcag aggatgagaa aaagctcttt cttagtctga tttcggtctc tgggattggt
    14581 cctgtatcag ctcttgctat tatcgctgct gatgacaatg ctggcttggt tcaagccatt
    14641 gaaaccaaga acatcaccta cttgaccaag ttccctaaaa ttggcaagaa aacagcccag
    14701 cagatggtgc tggacttgga aggcaaggta gtagttgcag gagatggcct tcctgccaag
    14761 gtcgcagtgc aagcaagtgc tgaaaaccaa gaattggaag aagctatgga agccatgttg
    14821 gctctgggct acaaggcaac agagctcaag aaaatcaaga aattctttga aggaacgaca
    14881 gatacagctg agaactatat caagtcggcc cttaaaatgt tggtcaaata ggagcagaga
    14941 atgacaaaac gttgttcgtg ggtcaagatg accaacccac tctacatcgc ctatcatgat
    15001 gaggagtggg gccagcccct ccatgatgac caagcgttgt ttgagttatt gtgtatggaa
    15061 acctatcagg caggcctgtc ttgggaaacg gtactcaaca aacgccaagc tttccgagaa
    15121 gcctttcata gctatcaaat tcactcagtc gcagagatga ctgacactga attggaagcc
    15181 atgctggaga atccagctat cattcgaaat agagccaaga tttttgctac acgcgctaac
    15241 gcccaagctt ttctacagtt acaggcagag tacggctctt ttgatgccta tctttggtct
    15301 tttgttgagg ggaaaactgt cgttaacgat gttcctgatt atcgccaagc cccagctaaa
    15361 acacccttat ctgagaaatt agccaaagat ctcaaaaaac gagacttcaa gttcacaggc
    15421 ccagtcgccg tattgtcttt tctacaggct gcagggctag ttgatgacca cgagaatgat
    15481 tgtgagtgga aaggtcttaa atgatgtcta acaaaaataa gggaattctg atttttgcga
    15541 ttctctatac agtcctcttt gtgtttgatg gcgttaaatt gctggcttct ttaatgccat
    15601 ctgccattgc aaattatctt gtttatgtag ttttagctct atatggctcc ttcttattca
    15661 aggatagatt gatccaacaa tggaaggaga ttagaaagac taaaagaaaa ttcttctttg
    15721 gcgtcttaac aggatggctc tttctcattc tgatgactgt tgtctttgaa tttgtatcag
    15781 agatgttgaa gcagtttgtg gggctagtcg gacaaggtct aaatcagtct aatattcaaa
    15841 gcacttttca agagcaacca ctactgatag ctgtttttgc ttgtgtcatt ggacctctgg
    15901 tagaagaatt atttttccgt caggtcttat tgcattactt gcaggaacgg ttgccaggtt
    15961 tactaagcat tattctggta ggacttgttt ttgctctgac tcatatgcac agtttggctc
    16021 tatcagagtg gattggtgca gttggttact taggtggagg ccttgccttt tctattattt
    16081 atgtgaaaga aaaagagaat atctactatc cactacttgt tcacatgtta agcaacagcc
    16141 tctccttaat cattttagct atcagtatag taaaatgaaa taagaacagg acagtcaaat
    16201 cgatttctaa caatgtttta gaagtagatg tgtactattc tagtttcaat ttgctgtaat
    16261 atgtgatgaa aatgccagta atgataccga gaaaaaagct gagaaacttt tcccagcttt
    16321 atttgttata gtcaaagcga atgacttgtt cctgtgcatc tacatgagca tggaccccaa
    16381 agggtacaat tgctcttgga gttgcgtggc cggcattcag attatagaca atcgggatat
    16441 tgctgtcaat gatatccaat agtgcctctt tatagtcgtc atggaaagtt tcatccatag
    16501 gttttccgac caagagtcca ttgatgaccg cgaatatgcc agtgtccttt aaagttagca
    16561 acatcttttt gaagtcttct ggcttaggct tttcttcgct tgtttcgagc aagaggattt
    16621 tcccttccca gtctgacaag tcagggaaaa gtttgtattt ttggcagagc tccgtgctat
    16681 ctgcgtatcg agagttgtca aagatatcgt agagggattc gaggcaacca ccgaggattt
    16741 tcccctcgaa tagtgccctt ccttgcaaca agtcaaaacc tgtatttgta tgactgacac
    16801 gaggtgttcc cagggccgtg ggactaaaat cagttcgttc ctcataccaa acgtcactag
    16861 ggcggatttc tgaaattctt cccgtctcaa tcaattcttt aaagtagtga aggctatagg
    16921 ctagcatttc tttgtctaat tcacaaatgt ctgctaaaaa ggattgacca taaaaagtct
    16981 tgattcctaa tttatgcaac atgaggtggt tcatggttgt atccgagaag ccaagaaaaa
    17041 ttttttgctt gataaccttt tggagttggt cattttcaaa aagataaggt agcaagcgat
    17101 aggtatcgtc tccaccgatg gcacatagga tcatgtcgat gctatcatca gaaaaggcat
    17161 gaatcaaatc ctctgcacga gcttcaggat ggtccttgat aaagtctaat ccttttaacg
    17221 aatggggcaa aaagatggga ttgagtccca gatccttgag acgttggata cccaagtcca
    17281 cttcgtgttt gacaaaatcc tctccgataa tgccactaga taaactaaca ataccaatag
    17341 tagaaaccat atctcatcct cctagaaata gattgagcct attttatcac aaaatataag
    17401 agaaagctat gtgggatttc agaagaatac ttttaatact cttcgaaaat ctcttcaaac
    17461 cacgtcagtt ttatctgcaa cctcaaagct gtgctttgag caacctgcag ctagcttcct
    17521 agtttgctct ttgattttca ttgagtataa ctcaaaaaag taagtgaaaa agcaaccgca
    17581 cctgcagttg cttttatcgt tttcttattc ttgatagcgt gttggtcctg cacttgcact
    17641 acggatattc aagcgtttct tgagatagaa gcgaagggct aggagggctg ctccaataat
    17701 agctagtggc aatggatcca gtactgggtt aaggctagct ggtaggaagc ttgttgcaaa
    17761 gaagacaagc aaccaaagga acacagaagc taggataact agtacagatt tccagaaagg
    17821 tggacgttga ctgcgatcca tatctggtcc atagtattgg taaacaaagt agtacatcaa
    17881 gtagaaggca aatccaccaa ccagtccaac taatagaaga gtaatcaatc catagccgaa
    17941 agcttgatct gccgcaaaga aggttgtgag ggcgctgaca agggcaaaga ggctagtgat
    18001 gaaaagagct gagtccataa tcatcagttt tgggtcatca ttttcttttg gatgctcttt
    18061 ttcgtactgc tctttgacag tgaagctatg agcccaatga gttggtgcgc cgtataggga
    18121 acgggcagtt gtacctttag attgctcctc aaggatttgg ggaatgactt cctcaaaaat
    18181 agtctggatt tcagcgtctg ttttcccatc tttgatgaat tgttgggtag caatgtggac
    18241 aaactcttgg tttttcttag ttaatttttg tagatcaatt tgagacatag gaactccttt
    18301 tagaaccatt ttttatggat gagatagaga gtgagcgaga cactcatagc aaaggcgata
    18361 aagacgatta accagaaggc atttggctct ccgtttaggg gaatttcatt atccttaaag
    18421 ttcatcccgt aggcagaaaa gaccatggtt gggatggaca tgacgatggt cacaagggcc
    18481 aaggttttca tgatgttgtt ctggttgtta gaaatgatag aggcaaaggt ctctgtcata
    18541 gaatgcaaga cgtttccata aatatctgcc atctcgatgg cctgttgggt ttcaatcagg
    18601 gtgtcttcaa gcaggtcttc gtcctcaagg tatttcttga tattgctggt tgaactggtc
    18661 aatttcttaa tcacgcgctc atttgttttg agggaggcct tgaaatagac gatagttttt
    18721 tccaattcca tgagctcaat caattcttca ttacgagttg attgatgcag ttgactttcg
    18781 atttgttcac tcttgcggtc gattgaacga agggctgtta ggtaaagctc tgcattgcga
    18841 taaagaattt gaaagataaa acgtgaacgc atgaaggtat agaaattacg caatcgacgg
    18901 ttgataaaga catcaaggac aggtagtggt tccaaacacg tagtgataat ggtttcctca
    18961 gtgatgataa taccaagcgg gatggttacg tagtaggtgc ggttatttct ttcctccgtg
    19021 accggcacgt ctacgataat cagggtatac tcgtcttcaa tggtaatacg agacatttct
    19081 tccgcatcga gcggtgctcg aaggtcggca atatcaatat cgaaggtgtt ggcgatttcg
    19141 agtgattcat tttgagtcgg attgacgaga ttgatccaag tacccggttc aagcgtatcg
    19201 atctctttaa attcagttgt tgtagagaga aaaacttgtt tcatatccct tatcctttct
    19261 catttttcag attttttcac actgtactat tatactacaa aatcggcctt ttgggtaata
    19321 gaatctcact ttttttggta taatggtaag caataatgga ctagaaagaa caaagatgca
    19381 agataaaatt gtcattcatg gggcgcgtgc ccataattta aaaaatattg atgtggagat
    19441 tccgcgagac aagttggttg tcgtgactgg cttgtcaggt tcagggaagt ccagtttggc
    19501 ttttgatacc ctctatgcgg agggacaacg tcgctatgta gagagtttgt ccgcctacgc
    19561 tcgtcagttc ttgggaaata tggaaaaacc agatgtagat gccattgatg gtctcagccc
    19621 agctatttct atcgaccaga aaacgactag caaaaaccct cgctcgacgg tgggaacaac
    19681 gactgaaatc aatgactatc tgcgtctcct ctacgcgcgt gtggggacgc cttactgtat
    19741 caacgggcat ggggctatca aggcttcttc tgtggagcaa atcgttgata aggttttgga
    19801 gttacctgaa cgccagcgct tgcagatctt gtctcctgtc atccgcaaga aaaaaggcca
    19861 acataagagt gttatcgaga aggttcagaa agacgggtat gttcgtgtcc gtgtggatgg
    19921 ggaagtctat gatgtgaccg aagtgccaga gttgtctaag agcaagcaac acaatatcga
    19981 tgtcgtggtt gaccgtattg ttatcaagga gggcattcgt agtcgtctct ttgattccat
    20041 tgaggctgcc cttcgtattg cagaaggtta tgtcattatc gacaccatgg acaactctga
    20101 gttgttgttc tctgagcatt atgcctgtcc agtttgtggt tttactgtcc cagagttaga
    20161 gcctcgtctc ttttctttca atgctccttt tggctcttgt agtgagtgtg acggcttggg
    20221 catcaagctg gaggtggata ctgatttggt agtgccaaat gccagcaaaa ccttacatga
    20281 gggagcgctg gcaccttgga atcctatctc atccaactac tatccaaaca tgctagagca
    20341 ggccatgacg gcttttggag tggctatgga taagcctttt gaggacttgt cagaagaaga
    20401 caagaacttg attctctacg gctcagatgg taagaaattc catttccact atgagaatga
    20461 atttggtggc gtgcgcgata tcgacattcc gtttgaggga gttatcaata atatcaaacg
    20521 tcgttaccat gaaaccaata gcgattacac tcgcactcag atgcgcctct acatgaatga
    20581 gctgacttgc ggaacctgtc acggttatcg tctcaatgac caggccttgt ctgtccgtgt
    20641 gggtggtgag caagggccac atatcggaga aatttcagac ctgtctatcg cagatcactt
    20701 ggacttggtg agccagttaa ctttgtctga aaatgaagcc atcattgctc gtcccattct
    20761 caaggaaatc aaggatcgtt tgaccttcct taataatgtg ggtcttaact atctaaccct
    20821 gtcacgttca gcaggaaccc tttcaggtgg ggaaagtcag cgcattcgtt tggcaaccca
    20881 gattggttcc aacctatcag gcgtcctcta tatcctagac gagccgtcaa tcggtcttca
    20941 ccagagagac aatgaccgcc tgattgccag tctgaaaaag atgcgtgact tgggcaatac
    21001 tcttatcgtg gtggaacacg acgaagatac catgcgtgaa gctgattatc tgattgacgt
    21061 tggtcctggt gccggtgttt ttggtggtga gattgttgca gcaggtacac ctaagcaagt
    21121 ggctcgcaac agcaagtcta tcacaggtca gtacttgtca ggcaaacgtg tcatcccagt
    21181 accagaagag cgccgtgtcg gaaatggtcg ttttattgaa gtgataggag cgcgtgagaa
    21241 caacttgcaa aatgtcacgg ctcgctttcc actaggaaaa tttatcgcag tgacaggtgt
    21301 gtcgggttca gggaaatcga ctctaatcaa cagcattctc aaaaaagcca ttgcccagaa
    21361 gctcaaccgt aattcagaca aacctggtaa gtttaagact attacaggga ttgagcatgt
    21421 cgaccgtttg attgatattg accagagccc tatcggacgg acgccgaggt ctaacccagc
    21481 tacctataca ggagtttttg acgatatacg tgaccttttt gctcagacaa atgaagccaa
    21541 gattcgaggc tacaagaagg gacgcttcag tttcaacgtc aagggaggtc gctgtgaagc
    21601 ttgctcaggt gacgggatta tcaagattga gatgcacttc ttgccagatg tttatgtggc
    21661 ttgtgaagtt tgccacggaa cccgctataa cagtgaaacc ctagaagttc actacaagga
    21721 aaagaatatt tcgcaggtct tggatatgac ggtcaacgat gcggtagaat ttttccaaca
    21781 cattccgaaa attcaacgca aactccagac catcaaggat gtagggctag gctatgtgac
    21841 cttgggacag ccagctacca ccctttctgg gggagaagcc caacgtatga agttagctag
    21901 cgaactccac aaacgctcga caggaaaatc tttctacatt ctggatgaac cgacgacagg
    21961 gcttcataca gaggacattg ctcgcctgct caaggtttta gctcgctttg tagacgatgg
    22021 caatacagtc ctcgtcatcg agcacaatct ggatgttatc aagacggcag accatatcat
    22081 tgacttggga cctgagggcg gtgtcggtgg tggaaccatc atcgtaacag gaactccaga
    22141 agaagtagct gccaacgaag ccagctatac aggacactat ttgaaaggaa agttacatca
    22201 tgaataaacg cgtacaagca tttctagcta aaatgcaaga aaaagaacta gatggtgtca
    22261 tcatcaacaa tcttaaaaac gtctattatt tgactggttt ttggggctca aacggaacag
    22321 tctttatcag ccgtgaccgt caggtattag tgacagactc tcgctatatc atcgcagcta
    22381 agcaagaaac cagtggtttt gagattgtgg ctgatcgtga tgaattggct gtcattgcag
    22441 gaattgttaa ggacatgggc ttgactcgta tcggttttga agatgagatt tcagtgtctt
    22501 attaccaccg tatgcaggca gcttttgcag gtttgaactt gcttccacaa actcagtttg
    22561 tggaaggtct tcgtatgatt aaggatgagg cagagattgc agcgattcgc aaggcttgtt
    22621 ctatctcaga ccaagctttc cgcgatgcgc ttgactttat caaaccagga aaaactgaaa
    22681 ttgagattgc caacttcctt gatttccgca tgcgtgagtt gggagcatct ggcttatctt
    22741 ttgatacgat cctagctagc ggtatcaatt cttctaaacc ccatgcccat ccaatgcaca
    22801 aaccagtgga gttgggagaa gccattacca tggacttcgg ctgtctctat gaccactatg
    22861 tcagtgatat gacccggact atctatctag ggcatgttag cgatgagcag gcagagattt
    22921 acaatacggt tctaaaagct aaccaagcct tgattgacca agctaaggca ggcttaggtt
    22981 tccgtgactt tgacaaaatc cctcgtgata ttatcattga ggcaggttat ggtgactact
    23041 ttactcacgg cattggccac ggtattggtc tggatatcca tgaggaaccc tactttagtc
    23101 agacttctac agaaactatt aagacaggta tggccttgac cgatgaacca ggtatctata
    23161 tcgaaggcaa atatggcgtt cgtatcgagg atgatatcct gattacagag acaggttgtg
    23221 aattattgac cctagctcca aaagagttga tagtcattta gttgtttgtc aagaaaaaag
    23281 tacgaaaatg acgaaaaaag tcaaaaaaaa tt
//
LOCUS       DAVBBS010000033        22314 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVBBS010000033 DAVBBS010000000
VERSION     DAVBBS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22314
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.033"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(160..2118)
                     /gene="ftsH"
                     /locus_tag="VQU32_001250"
     CDS             complement(160..2118)
                     /gene="ftsH"
                     /locus_tag="VQU32_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET2472707.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEETGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMFGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNREIHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            complement(2134..2676)
                     /gene="hpt"
                     /locus_tag="VQU32_001251"
     CDS             complement(2134..2676)
                     /gene="hpt"
                     /locus_tag="VQU32_001251"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET2472708.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKAREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            complement(2680..3957)
                     /gene="tilS"
                     /locus_tag="VQU32_001252"
     CDS             complement(2680..3957)
                     /gene="tilS"
                     /locus_tag="VQU32_001252"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET2472709.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEEEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ESIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            complement(3954..5222)
                     /locus_tag="VQU32_001253"
     CDS             complement(3954..5222)
                     /locus_tag="VQU32_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HET2472710.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            complement(5342..5710)
                     /locus_tag="VQU32_001254"
     CDS             complement(5342..5710)
                     /locus_tag="VQU32_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET2472711.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            complement(5703..5969)
                     /locus_tag="VQU32_001255"
     CDS             complement(5703..5969)
                     /locus_tag="VQU32_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET2472712.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            complement(6027..9536)
                     /gene="mfd"
                     /locus_tag="VQU32_001256"
     CDS             complement(6027..9536)
                     /gene="mfd"
                     /locus_tag="VQU32_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET2472713.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYAQHAFIHQLKENG
                     YQKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERELAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPIQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKEFKEENSI"
     gene            complement(9537..10106)
                     /locus_tag="VQU32_001257"
     CDS             complement(9537..10106)
                     /locus_tag="VQU32_001257"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET2472714.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVKYYLQEKNFEKTMQRYNG"
     gene            complement(10177..11292)
                     /gene="ychF"
                     /locus_tag="VQU32_001258"
     CDS             complement(10177..11292)
                     /gene="ychF"
                     /locus_tag="VQU32_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET2472715.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIIEFRFNV"
     gene            complement(11376..11570)
                     /locus_tag="VQU32_001259"
     CDS             complement(11376..11570)
                     /locus_tag="VQU32_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HET2472716.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            complement(11635..12771)
                     /gene="dnaN"
                     /locus_tag="VQU32_001260"
     CDS             complement(11635..12771)
                     /gene="dnaN"
                     /locus_tag="VQU32_001260"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET2472717.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            complement(12930..14291)
                     /gene="dnaA"
                     /locus_tag="VQU32_001261"
     CDS             complement(12930..14291)
                     /gene="dnaA"
                     /locus_tag="VQU32_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET2472718.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYNYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            complement(14504..15262)
                     /locus_tag="VQU32_001262"
     CDS             complement(14504..15262)
                     /locus_tag="VQU32_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET2472719.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            complement(15320..16501)
                     /locus_tag="VQU32_001263"
     CDS             complement(15320..16501)
                     /locus_tag="VQU32_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET2472720.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            16684..17163
                     /gene="rlmH"
                     /locus_tag="VQU32_001264"
     CDS             16684..17163
                     /gene="rlmH"
                     /locus_tag="VQU32_001264"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET2472721.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            17207..17280
                     /locus_tag="VQU32_001265"
     tRNA            17207..17280
                     /locus_tag="VQU32_001265"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17241..17243,aa:Arg,seq:tct)
     gene            17445..17570
                     /gene="comC"
                     /locus_tag="VQU32_001266"
     CDS             17445..17570
                     /gene="comC"
                     /locus_tag="VQU32_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HET2472722.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            17591..18916
                     /locus_tag="VQU32_001267"
     CDS             17591..18916
                     /locus_tag="VQU32_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2472723.1"
                     /translation="MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            18913..19665
                     /locus_tag="VQU32_001268"
     CDS             18913..19665
                     /locus_tag="VQU32_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2472724.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            19717..19788
                     /locus_tag="VQU32_001269"
     tRNA            19717..19788
                     /locus_tag="VQU32_001269"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19750..19752,aa:Glu,seq:ttc)
     gene            19794..19867
                     /locus_tag="VQU32_001270"
     tRNA            19794..19867
                     /locus_tag="VQU32_001270"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19828..19830,aa:Asn,seq:gtt)
     gene            complement(19908..20450)
                     /locus_tag="VQU32_001271"
     CDS             complement(19908..20450)
                     /locus_tag="VQU32_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET2472725.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            20629..21396
                     /locus_tag="VQU32_001272"
     CDS             20629..21396
                     /locus_tag="VQU32_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HET2472726.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            21481..22161
                     /locus_tag="VQU32_001273"
     CDS             21481..22161
                     /locus_tag="VQU32_001273"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HET2472727.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFVAAISTNMIFAKLPSGRH
                     PESRWAWLKSRAEINGIIAVLAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMV
                     TTLTTWNSRIGAFFLLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQT
                     ISINKSFS"
BASE COUNT         6901 a         4110 c         3643 g         7660 t
ORIGIN      
        1 acagtccctt ggacttcttc atacaattct ttaatcataa ttttcccctt tctagataca
       61 gtctaacaga ttagaaaaca catttgttcc attttctcca ttctgttccc tttagctcct
      121 aaactgcaca aaaaagagag gctccagcct ctctcagggt tatttttcgt cattcatttt
      181 tgactttact tcatcatagg atagtgcatg agattcctct tctactgctt caggcatctt
      241 tcctgtttcg taaagagctt taatttgtgt actatccaat gtttcgtatt tcaataatgc
      301 ttctgcaatt aacttgtgaa tttcacgatt tgactgaata atttcagcag ctttatttcg
      361 tgcctcattt aataatgaac gaacctcttc atcaatttca taagctgttt gttctgaaat
      421 tgatttttga ggactctgtg caccaaacat agcatggttt ccttcatatt gtactgggcc
      481 aagtttttca ctcataccgt actctgtaac cattgcacgc gccatttgtg tcgcttgttc
      541 aaagtcgttt gaagctcctg tagtttggac attaaagata atttcttcag ctacacgtcc
      601 acccattaag ccagccaatt gctctttcat atcttcttta gatagaagca tttgatcctc
      661 tttaggaagt gcaatcatgt atccgcctgc acggccgcgt ggtacaattg taaccttatg
      721 gacaacgcga gcattcgaca agactagacc aacaatggta tgtcctgcct catggtaagc
      781 aaccaattct cgttcttttt gtgaaactgt cttatctttc ttagaaggtc cagcaataac
      841 tctatcttct gcttcatcaa tatctgaagc atcaattatc gatttattgc gacgagcagc
      901 aactaaagct gcttcattca agacattctc taaatcagca ccaacaaagc ctggagtttg
      961 ttgagccact aatttcaaat caacatcttc tgctaaaggc ttgttcttag cgtgaacttt
     1021 caagattgct tcacgacctt taacatcagg acgaccaacc aatacttttc tatcaaaacg
     1081 tcctggacgc aaaagggcag gatcaagtac atctgaacgg tttgtcgcag cgatgacgat
     1141 aatcccttca tttccctcaa aaccatccat ctcaatcaaa agttggttca aggtttgttc
     1201 acgttcgtca ttacctccgc cgagaccgac tccacgttga cgtccaacag catcaatttc
     1261 atcgataaag atgatagctg gtgctgcttt tttggcatcc tcaaaaagag agcgaacacg
     1321 actagctcca actccgacaa acatttctac aaagtcagaa cctgagatac taaagaatgg
     1381 aacacctgct tctccagcga ctgccttagc aagcaaagtt ttacctgtcc ccggaggtcc
     1441 ctccaaaaga acacctgctg gaatacgggc tccaagtttt gtgaatcgtt ttggatcttt
     1501 taagaactca acaacttcaa ctagttcttg tttttcttcc tcagctccag caacatctga
     1561 aaatcttact ttaatatctt ctttatttgc tgctttagcc ttactacgtc caaaactcat
     1621 tggattacgg ccattgcctc ctcccatatt tcccatcata gagaatagga agaagaatag
     1681 aattccaaat ggcacaatgg atacgagtag attaatccat ataccacttg aactttcatg
     1741 cttaacagtt acttctgctt tatggtcagt agcaagtttt tgcaattctg atacggtagt
     1801 atctgcagga agaatagtgc tggtaaattt ctctacctta gtaacagatg gcgtgaaaaa
     1861 ctgaatacct gtttcttctt tacttgtttt aggattttta tagacaccag aaacttcgat
     1921 aacactacca tttggttggt aagttaattc ttttacatta ccatcggtaa tttcttgtac
     1981 caactcagta tagttgattt gctgacttcc tcctgagtta ttcccagaat agaaatactg
     2041 gaatcctgtc acaaggaaaa agataaataa taaccataga aaaggatttt taattaaacc
     2101 attattttgt tttttcatta aagattattc tttctaattt gaatacactt cctctttcaa
     2161 tactccaata taaggaagat tacgataatt ttctttgtag tctaaaccat aacctactac
     2221 aaactcattt gggatagtaa agcaggtata gtctgcctca atttctacaa cacgtccttc
     2281 tggtttatcc aacaaggttg caattttaac agaagctgct tctcttgctt taaacatatc
     2341 tcgcaaattc ttcaaagttt gacctgtatc aatgatatct tctacaaata gaacatgtct
     2401 tcctttgata tcttgagtca catcttgttt aatattgata acaccactac ttgctgttcc
     2461 accatggtag ctagaaacca tcatgaagtc catttcaata tgtgtatcaa tatgtttgac
     2521 caattcagcc ataaaaggaa tagatccttt taaaatccca actaagattg gattttttcc
     2581 tgcatagtct ttagttaatt gagcacctag ttttttagct gcttctgtaa tttcatcgtg
     2641 tgaaacgagg acttttttaa tatcgttttc taacattttt tacctatcta ttttttctat
     2701 ataaagtaca gtgttcatta tatcattttt cgttttttta ctcaaattat tggtcgcaat
     2761 tcccaaaatt gagacaattt caccaaattg ctcaataata agagcagagt ttctcttttc
     2821 catagggatt ttcaaatcaa taaataaacg tctgagtttt tttctatgcc cattttgaat
     2881 caaaacatct cctgtttttc gatgacgaat gtgtatggat gtttcacgtg aaacaggtat
     2941 ttgttgaatt gattcacctt ctaatggaag tccaaaggaa aataaatatc cttgataagc
     3001 tacctgattt tgatagtgta acacaagttc atcttcctct tcatcagcct gcggactgat
     3061 tttacaaatc tgaaactgtt ggtactcttt tatcaattca tagccatttt taatcggatg
     3121 acgatactgg cttttagatt ttaaaatctg ctgaacttca gcaaactgag cttttgtaag
     3181 attcaaatct ggaaaacgat tcagataagt ttgaagtaaa actctttgtg tagactcaga
     3241 gtaagaaaat aactgctgta aatcttccac attaatattg ttagataatt cagctattgc
     3301 caaatcataa tctaaaattt cattgccaat gcctaagatt gcatccctaa atcgaggatt
     3361 ttctttttcc aattctggta agtaagagtt tcgaatacga tttcgaaaat aatgattctc
     3421 ctgatttgat gtatcttcaa agtgaaaaat tgatggaaag tcttttttct gaaaatgcaa
     3481 gaagggacga atgatttcta tctctccgac tacttgcttc tccttaattc ctgatagata
     3541 gcgcaagcga gttcctcgaa tcaagcgcat caaaatcgtt tccacctgat catcagcatg
     3601 gtgggcagtg actaaagctg tcgcacctgt ctttttcatg acctcttgaa aaaaatcata
     3661 acgaaaattt cgtgcacgcg cttctgaaaa ttctcctgaa aaattgctga tataaatagg
     3721 aagctctgct tcagcagcca acttccttaa ttccttttct tcccaatctg attcaattct
     3781 ctgcttatga ttcacatgag ctagaatcaa ttcaatctct aactcttttt gataagtaga
     3841 caatacctta aatagaaaca tggaatctaa tccaccagaa agagctagaa ccgccttagc
     3901 atgctttttg aaatatccct tcttgagaaa atgatttaaa aaatctggtt ccctcatttt
     3961 agaacctcat aaacatcctt ggctatctta gaaatcgtat cataatcaga attcttagtg
     4021 aaaatagaaa gaataaatgg agaatctgca tagacaacac ccgtatcatg cttaaattca
     4081 tccgcatctc caattttatg agctacttta acagaaacac ctttggcaat tcgctgacta
     4141 tcaaaatctg ttttagtcaa agactctaac acaaatccat tttgattata aatagcttcc
     4201 ataaacttcc cggccatctt agaagaaatc aatttttctt ttggatccca atcatctccc
     4261 ataatggcag acatcttgga tttgaatgtg gcatcagatt ggtttgaaat gtaatatccc
     4321 aatagattat gagctacatt atcagattct tttgatactt tcgtaattaa atcctttaaa
     4381 gaatattctt tattatcttc ttttttagga agactaccac ttccctctgg tttataagaa
     4441 cctggaaaat cattgactgc agatacgtat tttacagtcg tatctaactg ataaagaccc
     4501 tcatttattt tttcttgcgt ataatagaga taagagagtt tcaaaacgct ggctgcatac
     4561 atcttttcat cttgattgat accagcttct tttccagtag tcagttgctt aacataaata
     4621 gagaaagaat ctttctgata tttttcagat aacatttctt gaactttact catccgatta
     4681 tcttcttcag aagttgattc tttagctacc catccagcct gatcaatatg tagaaattct
     4741 cttccttcta caaacatggt cttgtcgatt gatacttgcg aataagctga taaggatgat
     4801 ttcacttctt ttaaatcata aggactattg tacagtttaa agtcagattc taaccatgct
     4861 ttttttattg ttggagttac ctctgattga tcatataaaa atcgtttgtc cgcagctata
     4921 aattgatgat ttgatagctt aaatactgga attccttgtt tatttaagcg ccactcggtt
     4981 atttgaaaac ttgttttggg agtcaatttc ccagattcca ctactaaatc ttcattcgca
     5041 taaacaggaa cctctccata aaccatggga gaacttaatt tttctctaaa ataaatacca
     5101 aagtcagatt gtgaaaggta ataaatttct ttcgaagtat agacgacttc tttttctgtg
     5161 ctaacgactt ttgaaatggt caaaaaactt ggtagcaaca aaataattaa gaatttacgc
     5221 attcttcctt tccttttctt cttgtaaacg caataattga tttttatcag ccaattcttc
     5281 cagcttttca tctgacaaac tcaaaaattg ctctattacg tctaataaat tttccatttt
     5341 atcacctttg aagcaagtca ggaatcgtat aaactttttc cctcgactta gaataatagt
     5401 acttcgctcg tgtatatttg gcagcataat cttcatcttt caacttggta gcaaatgctg
     5461 tctccttatc cttttcatca ctcaaagttt gatactgagt ttgcaagtct gctaattgct
     5521 gacgtctttg gagtaattgc tgataactct gcgctaaatt aaaagttggc aagataaata
     5581 atagcataat caaaatcaat acccctccca taaaacgatt ccgtttttgt cgttctttca
     5641 tcaggtagcg acgacgttgg tattcatttt gaataaaaga attattcaat tgtacaatat
     5701 ttttagacat tttcttctac ccgtgtttca ctgataattt catacattcc tgctgcatct
     5761 tctttttttg tactatcttt catctctagt acttttacaa gcagcaactt attgccaaag
     5821 cgaatttcaa cttggtcatt aactttcaag tccgttgaac ttttggccaa gattccatta
     5881 accttgattc tacctttatc tgctacttcc tttgcgactg tacgacgctt gataattcgc
     5941 gatactttta aatatttatc taatctcatc cttattacct caatttatta ttgtaccatt
     6001 tttatctatt ttatagaaga aaaatatcaa atggaatttt cttccttaaa ctcttttatc
     6061 tctaataaac tttctccaaa aatcagcaga ccttctaaaa tttcataatc tttcttattt
     6121 tggacatcaa atacaagctc cattaatccc ttattctcag cgatgcctgc ttttaagttc
     6181 gttacggata aagctttaaa ataatcttga gctaaaaaca atcgttgagt gactttttca
     6241 aattgaattg taattttatt atcttttctt tccacacgtt gaacaaagac cttgtccaag
     6301 tatgatttaa ccaaaccaat ctctaaaaga taggctacta catctgggta ttctccaaaa
     6361 cggtctatca actcctcttg taactcttca taattgacac ggttgtcaat ttgacgaatt
     6421 ttcttgtaaa tttcaatctt atgtcgttga tcagaaatat aagtatcagg aagataggca
     6481 tcaatttgca aaatcaactc agcattccct ttggttcttg tgttagcgtt agcattaccg
     6541 tttcgtttag caatagcttc ctctaataac tgcgaataca attcaaaacc aacagaatca
     6601 atgaaaccag actgggattt tcctaaaaga tttcctgctc cacgaatcga aagatctcgc
     6661 attgcaatct taaagccaga gcccaattct gtaaatcctt taatcgcttc taatctcttt
     6721 tcagagactt cactgattga tttttctgga cgatacatga gataagcata agcaatacga
     6781 ttactacgac cgactcttcc tcttaactga tataaggttg acaagcccat atggtccgca
     6841 ttttcaataa ataaagtatt agcatttgga atgtccaccc ctgtctcaat aatagtagtc
     6901 gtcaccaaga tatcgtattg tccctcaata aagtctaata gagtattttc caactggact
     6961 tcactcattc gaccatgaac atatccaatc gaagcctccg gaatcaactc ctgtaattct
     7021 gaaaccttct gaacaattgt gtcaactttg ttgtaaagat aataaacttg acctccacgc
     7081 tccatttcac gcaagacagc atcacgaatg acactatcat tcttttccaa aacataggtc
     7141 tgaacaggat agcgattagt cggcggagtt tcaataacag ataaatctct gattcccagc
     7201 atagacatat ggagggtacg agggattggc gtagcggtca aggttaggac atccacttgt
     7261 ttcttcagtt ctttcaaagt ttccttatgc ttgacaccaa atcgctgttc ctcatcaata
     7321 atcatcaagc ccaaatcagc aaacacaaca tcttttgaca aaacacgatg tgttccaatc
     7381 aaaatatcga cttgaccgtt tttcaatttt tcaagtgttg cagtctgctc ttttttactt
     7441 ctaaagcgac tcaacacatc aatattaact gcaaaatttt ggaatcgttc cttaaaattc
     7501 gtatagtgct gttgcgctaa aaccgtcgtc ggaactagaa tgacaacctg tttgtgatca
     7561 ttgactgctt taaaggctgc acgcatagca acttcagtct ttccaaaacc aacatcccca
     7621 actaaaagtc gatccattgg ctgagaagcc tgcatatccc tcttgatttc ctcaatacta
     7681 cgaagttgat catccgtttc aacataaggg aaagcatcat caaaggcatc ttgatcatca
     7741 tcatcagctg agaaagcaaa acccttcaac tgactacgtt cagagtagag tttgattaaa
     7801 tcatcagcta tatcctctac ctggttctta accttttgct tggccttttt aaaatgaccg
     7861 tcatttaatt tattgagttt tggagcttta ccatcacttg aaatatattt ggacagtaga
     7921 tgaatctgtt ccacggggat agaaatttga tcaccatttt ggtattggac actgacataa
     7981 tcgcgatgaa ttcccttgat ttcaatggtt tcaattccta gatattgacc aatcccatgg
     8041 atatgatgga caacatagtc ccctttttca agttcattgt aatcttttaa tctctctgca
     8101 tttgaaacat gttgtcttcg aaaacgacgc tttaatttct tttgaaaaat ctcatgttca
     8161 gttatcaata aaatcttttc atctacaaaa tgaaaaccat gtctgagatt accctctatt
     8221 aaatttacag attctttaca gatacttgtc ttatctctag aatccaattt aatctgatat
     8281 tcctctaaca tatcctccaa tgttttactt cccattgaat tgctagactg cagaataatg
     8341 gtgtaatcca tttttttata tcgttcaatt tcttctttta gaaaagaaaa ctgattgaaa
     8401 aattcctgta taggatattg attgaattga taaattttgt caaatttgag atttcctaaa
     8461 cccttttgaa gattagagaa aaaggtcact ggactttgtt ttttatagat ttgttcaata
     8521 tcagaaaaat actgcatatc agaaaatgct ttactattct gtaattcttc tgtaaagtac
     8581 tgcgctaatt ctctttcaaa gacttcatac tgattcatca atttttgata atcatcaaag
     8641 aatattggag tatctttttc aatataatca aagacagtcc atgtcttatc atagcacaaa
     8701 gataaaaact tccgagaatc tgcatgactt tgtttttggt gaaaacttga aagaatttct
     8761 tctaggtatg atttcaaaat aggtgataaa gtttttgaaa tttgtttttc taaagctgac
     8821 tgtcctcgtt gataatcctt ttctctcaaa agcatatcac tagctggaaa gatagtgagt
     8881 tctgtcttat tttctttcga taattgtgtt tctacttcaa atgacctgat accatcaatt
     8941 tcatcaccaa aaaactcaat tcgacaaggt tctaactggg atatttcaaa aatatctaaa
     9001 atatctcctc gaagactaaa ttcgccctga gtttgtactt gagtaacttt ttgatagcca
     9061 ttttccttta actgatggat aaacgcgtgt tgagcatatt cttcaccaac tgagattttt
     9121 acaatactat ctttgaatgc attgggagac ggtaaaatca atcgacttgc tgcgatatta
     9181 caaactaaaa tccctttctt agatgaatca gtcaaaaaac gcaaggcttc aacccgtgaa
     9241 ataatttttt cctgtgaaga catcaaaaac tccaccatag gagcatcatc taccaaaaat
     9301 ggatagacga gttcctcacc caagatagaa ataagatcac taacaagtcc ttctgcttct
     9361 ccataagttg acgtcaataa cacaatccta tcttcttttt ctaaactgct tgcaattgca
     9421 agagccttag tagatgttga taaaccaagt attagttgtc ttttcttatc tgttaaattt
     9481 tgatgccatt ttttaatctg atcattttct gagaataaat ctaataaggt caccatttat
     9541 ccgttatacc tctgcattgt tttctcaaaa tttttctctt gtaaatagta ttttacagaa
     9601 tcgtcaactt tgtcaacaga ctgtaaaata ccgatataat catccctgtc aaacttactc
     9661 aaaacatgat gaacaactga cataccattt ttaggtcttc caattccaat cttaacacgg
     9721 ttaaagacct gagttcctat atgttgaata atagacttga taccattatg accacctgct
     9781 gagccttttg ctcttaaacg aattttccca acttccatgt caagatcatc gtaaatgata
     9841 agtaaatcgt caatatccaa accatagtaa gttaataaag catgaactgc ttttccactt
     9901 tcattcataa aggtcgttgg tttaaccaga taaatttttt ctccatttag gaaaaaggat
     9961 gctaggtcag cttgaaatat cttatcgtgt gtaaaagtga cattctgttt cttcgctagt
    10021 tgatcaatca acataaaacc aacattgtgt tttgtttcaa aatatttatc ccctggattt
    10081 cccaaaccta caagtaattt ggtcatttat ttttcctttc aaaagccaaa agggttggaa
    10141 tttttttcca acctaattga caccatttat taatttttag acattaaagc ggaattctat
    10201 gatgtcgcca tcttgaacga tatattcttt tccttcttca cgcaagcgtc cagcttcttt
    10261 tacggccttt tcagatccgt atttcactag atcttcatat gacatggtta ctgcacgaat
    10321 aaagcctttt tcaaagtctg agtggataat accagctgct tgaggagcct tcataccacg
    10381 tttgaaagtc caagcgcgaa cttctttttc accagctgtg aagtaagttc ccaatccaag
    10441 caagtggtaa gctgcacgcg tcaacttatc tacacctgat tctgtcaaac caatggcttc
    10501 aagaaactct tttttatctt catcatccaa ttcagaaatt tcttcctcag cacgcgcaga
    10561 aataacgact acttcagcat tttctgtcgc tgcaaattca cgaatttgtt tgacatagtc
    10621 gatagagtca ggttctgaaa ccacatcctc gtccacatta gctacataaa gaactggttt
    10681 agtcgtcaaa aggaaaagac ctttgacaac cttttgttcc tcatctgtaa attcaatggt
    10741 acgagctgat ttcccgtctt ctaggactgg tttaatcttt tgaagaacat tgaattctgc
    10801 tactgattct ttatcttttt gcgtacgtgc catcttttct acacgcgcat atcgtttgtt
    10861 cactgattct aagtcagcaa gaattaattc cagattaatt gtatcaatat ctgcaagtgg
    10921 atctacaaag gcgtcttcac gtccttgctc gcgcatcaca ttttcatcat caaaagcacg
    10981 aactacgtga acaatcgcat ctacttcacg aatattggcc aagaatttat tccctagccc
    11041 ttctcctttt gaagctcctt ttacaatccc tgcaatatcc gtaaattcaa atgttgtggg
    11101 aactgtcttt ttaggagtta tcatttcagt tagtttttgt aggcgttcat ctggaacttc
    11161 caccattcca acatttggat caatcgtcgc aaatgggtag tttgctgcct ctgctcctgc
    11221 ttttgtaatt gcattaaata gtgttgattt accaacgttt ggcaaaccaa cgatacctgc
    11281 tgtcaaagcc atatttctca ttctccgttt tcatttcaat ccctaatatt ataacacaaa
    11341 aagggataaa gtgctaaccc tctaactaag ggttctcagt caataatttt attcattttt
    11401 cgctcaaaat catatcgccc catcatgaca acatgctcac aattactgca ttttattttg
    11461 atatctgctc ctacacgtgt aatttcccaa cgattagcct ttttaccagt cgacttgatt
    11521 gtacaagcgt gtgatttttt catctcaaca aaatttccaa cttgatacat actactctcc
    11581 ttttcttttt cgattatatc ataaaagagg cgagccaggc tcaacctctt tcacttaatt
    11641 tgtacgaact ggtgtaatga gctgcatgaa gtcttcgtca gtatctgctg gcacaagagt
    11701 aaatggacga acagctgaga taaagctaat ggtcaccttt tcgctattta aagctttaag
    11761 agaatcaatc aagtaagttg ggttgaaact aatggtcaaa tcttcaccag taacctgatc
    11821 agtatcgatt tcttcgttta ctttaccaac ttctggagag tgaacatggg cgctaacaac
    11881 cccatcctta atttcaagtt tcacagtacc attttgagtc gcacttgata aaagacgggc
    11941 acgctccatt gactggcgta agtttaccac attaaaagta atagtagtgt taaagtctgt
    12001 tggaatcaag cgatctgtat caggatagtt tccttctagg agacgagtat agaagctaat
    12061 attttcgctt ctaaagagga tttggttatt ggcaaagaaa atctctacag tttcgatatc
    12121 atctgtaaat accgctgaaa attcgcgtag agaacggcta ggaattacga catcaaaatc
    12181 atcactattt ttttcaagag tcaatttttt ctggcttagg cgatgagagt ctgttgcaac
    12241 tgtttttaac tctttgtgtt gactcaatac gaagtggaca cctgttaaaa tcggacgact
    12301 ctcttgtgta cttgcagcaa aggctgtttc attaataatt ttcttgagta attttgtttc
    12361 aagtattaaa ggagtgcttg ctgaaatttc ttggattcgt ggatattgtt cgctatcttt
    12421 tccttttagg gtaatttctg atttgccact ggttaaaaca atttgatttt gttcaatttc
    12481 tttaaaatca agagttacat caggtaaact agatactaca ttgataaaga aagaagcttc
    12541 aagaaggatc gaacctaaag aagtaattaa caaaccagca tcttcatttt tttgagaaat
    12601 aaaattttca attgaaattt gaccatttga accaattaaa gtaatacctt cattggtcac
    12661 gtcaattttt actgttgata aaataggaat ggcattttta gaactaatag ctctcttagt
    12721 agtatttaat gcttgtagaa ataaattttt attaattgaa aaatgaatca tggattctcc
    12781 tttatttatt tttagtatta gaaagataat agtaataata gagtctgtga aatctgtgga
    12841 aaacagcctt taaacaagtc atatcaagtt ttttagcttg tttaaaaaat gtggataaaa
    12901 aagataaaaa actaaaagtt atccacaaat tatttgattt tctttttgat tgattcaatt
    12961 tctaaacgta aattatcgtc ttgatcaatc aaagatttta ttttggcatg ggcatgaatg
    13021 actgtggtat gatcttttcc cccaaattcc ttcccaattt ttggaagact attatctgtt
    13081 agttctctag ataaatacat ggctacttga cgggccaaaa caatattttg aaggcgtcta
    13141 cttcccttca tttctttgat actaacacca taaaagttac caacttcagt ttggatttta
    13201 tcaattggga tgacgagcat ttggctaaca tcttgtttgc gggctctaat ggcttctgca
    13261 gcaatatcaa tagtgatatc cttgattttt tttactctgg caattaaagt gatgtcgttg
    13321 atggctccct caagatctcg aacatttgaa tcaaattgcc cagctaggta ttctagagta
    13381 tcactttgga aattgtagcc taaatgttcc gttttacttt gtaaaatggc aatacgtgtt
    13441 tcaaagtcag ggggtgtgat agtttgtgtc aatccccaac taaaacgcgt gacaagcctc
    13501 tcctcgagcc cttctagatg ttttggacta cgatcactcg ttaggacaat ctgtttttgc
    13561 ttgtcatgaa gggcgttaaa ggtattgaaa aattcttcct gagttgcgac tttttttccg
    13621 ctgagtgact ggatatcatc gattaacaaa agatcaagac tacgataggt ctttttaaac
    13681 ttttccattt ccccaagtct taggtgatca agaaagtcat taataaagct ttcggcaggg
    13741 atatatttaa cacgcgcatt aggaatattt tttagaattt catttccaat agcgtttaat
    13801 aagtgagtct taccaaggcc tggtcctcca tagataaaaa gagggttata ggtcagagcc
    13861 aaatcttcag agacagctaa agcggctgac acagcccaaa catttccatc cccttgaata
    13921 aagttatcaa aggtatactt ttcttttaat cccgtatctg aataaggaat agatactaac
    13981 tttggactat agttataaag agttaaattt gtagcttctt caacttgtga gctagtcgta
    14041 tcttgaggtt tggtgaaaat atagtgggga gttatttcag cgtcataaat ttcaaaacca
    14101 gctactacaa taatatcttt tagttgtttt tcccagacca tttccatttc agagcgaggt
    14161 agaaatatag tggcaacatt ttcctctacc ttgatgagtt cagcttgaat agcatagaaa
    14221 tcatacatgg atcgagtcag tctttcttgt gcaaattcta atatacgatt ccaaaattgt
    14281 ttttctttca atcctttctc ctcctttact atttaaaagt ttaatactag agatatttta
    14341 ccatagatag caagaatttt ccacaagctg tgaaaaataa tccacaatgt taccaaactt
    14401 tatccacagg ttggggataa aagaagaaat tattgattta ttaagctttt tattgaaaaa
    14461 catagtggat aaccttgtga gataaaaaaa taaaagaaca gccttatttc aggctgttga
    14521 taattctact atattcttct tgatttgaaa aagaaataat gatttttcca ctgtcttttt
    14581 tagatagttt aatttctaca tctaatccga gtagttttct taactgtttt tcttcatttt
    14641 gtatgaaatg attagttttt tgctgtttct tttgtttttt ctctgtcaga agagcttcta
    14701 atttccttac agaaatatct tcttctataa tccgttgaaa gaaatagtct tgttgttcct
    14761 tatttaaccc aactagggaa cgcgcatggg cttgtgatag tttgccattt tctacttctg
    14821 aaagaatctg ttctggcaag gaaagtaaac gaatggagtt gctgatatat ggacgagact
    14881 tgcccatctt atctgcaatt tcagcatggg tgaatccttt ctctacgaga gattcatagg
    14941 cgcgtgcttc ttctattggg tttaaatttt ctctctgtaa attttcaata atggactgga
    15001 ccatcatctc ttggtctgaa atctgtttaa caacagctgg gatagaccgt agaccagcta
    15061 aaagtgaagc ccgatagcgt ctctctcctg caaggatttc ataaccaata acaggagatt
    15121 gacgaacaat aatcggttga atgaccccat tttctttgat agactgtgct agttcatcta
    15181 gtttttctct atcaaattct tttcggggtt gatagggatt tttttgtata tctgtgatag
    15241 aaatcatttc aaatttttcc atgattctac actaacacat cttttctctt atgtaaagct
    15301 ttctttacat agatgtcaat taagattcta aatcacctga actcttgtta agtttgatag
    15361 aggtagtttc ttctttcccg ttacgatagt aggttatctt aatggtgtct ccgatagaat
    15421 ggttgtaaag agcactttgt aagtctgttg atgaagcaat ctctttgtca tctacttttg
    15481 taattacatc gtatttttca aggtgaccat tggcaggcat attactttgt accgaacgaa
    15541 caactacacc agatgtaaca ttacttggaa tattgagtct tctgatgtcg cttgtactca
    15601 cattagataa attaaccatc tggattccca aagctggacg cgtcactttt ccgttttttt
    15661 ctaactgttc aataatattg atagcatcat ttgcaggaat tgcgaaacca agaccttcta
    15721 cagatgttcc tccatttgta gcaattttac ttgaggtaat tccgataacc tgcccttgaa
    15781 tattgatcag tgggccgcca gagttacctg ggttaatagc agtatcagtt tggatggctt
    15841 ttgtagaaat agcttgtcca tcttccgatt ttaaggatac atttctattg agactggata
    15901 cgataccttg agtgacagta tttgcatatt cagaacctaa cgggctaccg atggcaatag
    15961 cagtttctcc tacagttaac ttactagaat caccaaactc agctactgtt gtcacttttt
    16021 ctgaagagat ttttacgaca gcaatatcag agaaagtgtc agctccgaca atttctccag
    16081 gtactttagt cccatctgac aatcgaatat ctactttgct ggcgccattt ataacgtgat
    16141 tgttggtgac gatgtaagct tctttatcat tctttttata aataactcca gatccttcac
    16201 tagagattcg ctgagaatct gtgtcagtat catcattgcc aaatacgcta ttttgtctgt
    16261 ttgccgaata agtaataaca gaaacaacag catcttttac tttgttaaca gcctgtgttg
    16321 ttgaattttc gttcttatag gcagtttgtg taatagtact attgttgtta gagttgttta
    16381 cactactttt ttgagttagt tgagttattg aaaaactacc caaggctcca ctaaaaaagc
    16441 taatgacgat aacgactaat aattgaaacc attttttgta aaatgttttt agatgtttca
    16501 tatttgcctc catatgtttg aattactgaa agtataaact gactagctta attataactt
    16561 aaacacaaaa gttttacaca aactgtggat aactcttttg aaactgtgat tttcttaatt
    16621 gaaatctatt ttttattttg tgaataagat gtgaaaaaat agagaatatg ttagaataga
    16681 gtcatgaaaa ttaaagttgt aacagttggg aaactgaaag aaaagtattt aaaagatggt
    16741 atcgcagagt attcaaaacg aatttctaga tttgctaagt ttgaaatgat tgagttatca
    16801 gatgaaaaaa caccagataa ggccagtgaa tcagaaaatc aaaagatttt agaaatagaa
    16861 ggtcagagaa ttttatcaaa aattgctgac cgtgatttcg ttattgtgtt agccattgaa
    16921 gggaaaactt tcttctcaga agaatttagt aagcagttag aagaaacttc tataaaagga
    16981 ttttctactc ttacttttat tattggggga agtttaggat tgtcatcatc tgtaaaaaat
    17041 agagccaatc tttctgtcag ttttggtcgc ctaaccttac cccatcagtt aatgagacta
    17101 gttcttgttg aacaaatcta tcgcgctttt acgattcagc agggattccc ctaccataaa
    17161 tagaaaattg acttttaatt gaatttttgg tagaataatt gtattaggtc tcatagctca
    17221 gctggataga gcattcgcct tctaagcgaa cggtcgcagg ttcgaatcct gctgggatca
    17281 atataatagc aaagctggga attttcccgg cttttttctt aaaaaagtac actttgggag
    17341 aaaaaaatga cagttgagag aattttatct aaaacgaaat tccattttgt ataatggttt
    17401 ttgtaagtta gcttacaaga aaaaacattt taggagattt tattatgaaa aacacagtta
    17461 aattggaaca gtttgtagct ttgaaggaaa aagacttaca aaagattaaa ggtggggaga
    17521 tgaggttgtc aaaattcttc cgtgatttta ttttacaaag aaaaaagtaa tgaaataagg
    17581 ggaaagagta atggatttat ttggatttgg gacggttatt gttcattttt taattattag
    17641 tcacagttac cattttattt gtaaaggtca aataaataga aaagaattat tcgtttttgg
    17701 tgcttataca ttactaacag aaatagtatt tgattttccc ttatatattc tatatttaga
    17761 tgggttaggg attgaaagat ttttatttcc tttgggatta tattcctatt ttcgatggat
    17821 gaaacagtat gagagggata gaggactatt cctaagttta ctactatctc ttttatatga
    17881 gagcactcat aactttctgt ccgtaacttt ctcctctata acaggagata attttgtttt
    17941 acaatatcat ttcccattct ttttcgttgt gacggtgtta acctattttg ttacattaaa
    18001 aatcatttac tatttccatt tggaactagc ctattttgac gaggactacc tttatccttt
    18061 cttgaaaaaa gtattttttg ctttactatt gctacatatt gtatctttcg tttcagatat
    18121 ggtaagtacg attaaacatt tgaatagttt tggaagtatt ttgtcatcta ttgtctttat
    18181 ctctctcctt ttgaccttct ttgcaatgaa ttctcataaa gttcaaatgg agaaagagat
    18241 tgctttgaag cagaagaaat ttgaacagaa acatttacag aattacacag atgaaattgt
    18301 tggtctgtat aatgaaatcc gtggttttcg acatgattat gctggaatgc ttgtcagcat
    18361 gcagatggca attgacagtg gtaatttaca ggaaattgac agaatttaca atgaagtttt
    18421 agtcaaagca aatcataaat tgcgttcaga taagtacact tactttgatt tgaacaacat
    18481 agaagactca gctttacgaa gtttggttgc tcagtcaatt gtctatgctc gaaataatgg
    18541 tgtagagttt acactggaag taaaagatac gattaccaag cttccaattg aactattgga
    18601 tttggttcgt atcatgagcg ttttattgaa taatgctgtc gaaggatcgg ctgatagcta
    18661 taaaaagcag atggaagtag cagttattaa gatggaaact gaaacagtta ttgtgattca
    18721 gaattcatgt aaaatgacga tgactccttc aggagatcta tttgccttag gattctccac
    18781 taagggaaga aatcgcggag tcggattaaa taatgtgaaa gaactactag ataagtacaa
    18841 caatattatt ttagaaacag agatggaagg cagtacattt agacaaatca ttagatttaa
    18901 gagggaattt gaatgaaagt tttaatttta gaagatgtta ttgaacatca agtgagacta
    18961 gagagaatat tggatgaaat ttcgaaagaa tcgaatattc caatatcata caagacaacg
    19021 ggaaaagtcc gtgaatttga agaatacatt gaaaatgatg aagtaaatca gctttatttc
    19081 ctagatatcg atattcatgg aattgagaaa aagggatttg aagtggctca gctcattcgt
    19141 cattacaatc cttacgctat tatcgtcttt atcactagtc gatcagagtt tgcgactcta
    19201 acctataaat accaggtatc agccctagat tttgttgata aggatatcaa tgatgagatg
    19261 tttaagaaga gaattgagca aaatatcttc tacacgaaga gtatgttact tgaaaatgaa
    19321 gatgttgtag attatttcga ctacaattac aagggaaatg atttaaaaat tccttaccat
    19381 gatattttgt atattgaaac aacaggggtc tctcataaat tgcgcattat tggtaagaat
    19441 tttgcaaaag agttttatgg taccatgaca gatattcagg aaaaggacaa acatactcag
    19501 cgattttatt ctcctcacaa gtcatttttg gtaaatatag gcaatatcag agaaattgat
    19561 cgaaaaaact tagaaattgt tttctatgaa gaccatcgtt gtcctatttc aagattaaaa
    19621 attagaaaat taaaagatat tttagagaaa aaatctcaaa agtgattgac aattagcaag
    19681 aaattgatat aatggttata tctgctacag atagaaggtc cgttggtcaa ggggttaaga
    19741 caccgccttt tcacggcggt aacacgggtt cgaatcccgt acggactatt ttatccgcca
    19801 tagctcagtt ggtagtagcg catgactgtt aatcatgatg tcgtaggttc gagtcctact
    19861 ggcggagtat agataaaagg gaacacagct gtgttcctct ttttgtatca atttgtatca
    19921 ccaagcattt tcataaggaa gtctgttatt tcttgagaac tttctttttt tccatgtgca
    19981 atccaagttt ggcagacacc aaaaagtgca tgagttagat agatgctact atattctaat
    20041 tcggtggtat ttagattcag ttgcataaat cgcttttgta aatctgtact aagcatgata
    20101 tgaagtttat ttcgtaagaa attttggatt tctttagtcc cattttcaga aagaagggca
    20161 gccagaagtg gttctgactc tagatattca aaaacttcta aaatagcgtc tcttttgtga
    20221 tgagcatgtt tttgaaaaat atattcaaat gtatggaata gcttgctttg atagtgctca
    20281 atcatatcat acttatcctt atagtgagta tagaagctgg aacgactaat tccggctttt
    20341 tctactaatt tgacagtaga aattttatca aatggctgtt ccatcagtaa ttgtaccata
    20401 gcattttcaa tagttcgctt tgtttttaag cgtttgttac tttcttgcat atttcctcct
    20461 tgtaaacaaa ttagactata tgtttaaaaa tagatttttt atcttgtaat ttagattttt
    20521 taatgtataa tctattatat caaaatttta gacaatatgt ttaaaaaagg agaaactaag
    20581 tttaaagaat ggaaagcaat ttaaaaaaaa ccaaccttta ttattgtcat gatcgggatt
    20641 tctcttattc cagatctgta caatatcata tttttgtcat caatgtggga tccatatggg
    20701 caattgtctg acttacctgt ggcagttgta aataatgata aagaggcttc ctataatggt
    20761 aatactatgg caataggaaa agacatggtg tccaatttaa aagaaaataa aaccttggat
    20821 tttcattttg tagatgaaga ggaaggaaag aagggattgg aagatggcga ttactatatg
    20881 gtagtgactt taccaagtga tttatctgaa aaaacaacta cattatccaa tattcaatcg
    20941 acagcagctt atcaatcatt gacaagtgag caacaaactg agataagtga ttctgtatct
    21001 caaaattcaa ctgatagtat tcaatcggct cagtcaattg tagctttagt acaagattta
    21061 cagggaagtt tagaaaactt acaaaatcaa tcttctaatc tttcgacttt aaaaaatcaa
    21121 tctaatcaag tatcacctat tacttctact tctttgatag gattgtcaag tggattaaca
    21181 gagatacaag gagatgttac tagcaaatta gttcctgcca gtcagtcgat tgcatcaggt
    21241 gtaaacgcat atactacagg tgttgataaa gtttctcagg gcgcaagtca actaagtgaa
    21301 aaaaatgcca ccttgacagg tagtttggat aaactagttt caggctcaaa caccttgaca
    21361 caaaaatctt ctagattgac agcaggagtt ggttaattac aatcaggatc tgggcaatta
    21421 gcagacaaat ccagtcagtt actttcaggt gcttctccat tagagaatag agctaataaa
    21481 ttggcagatg gatctgggaa actagcagaa ggtggaacaa agttaacttc tggattggaa
    21541 gatttacaga caggacttgc ttctttagga caaggactag gtaatgctag tgatcaactc
    21601 aaatcagtat caacagaatc taaaaatgca gagattttgt caaatccact caatctttca
    21661 aaaacagaca atgatcaagt tcctgtaaat ggaatcgcaa tagctcctta tatgatatca
    21721 gttgctcttt ttgttgcagc aatatcaaca aatatgatat ttgcgaaatt gccttcagga
    21781 cgtcatccag agagccgttg ggcttggttg aaatctcgag ctgaaataaa tggtattata
    21841 gctgttttgg caggaatttt ggtatatgga ggagttcagc ttattggttt aactgctaat
    21901 catgagatga gaatatttat tctcatcatc ctaacaagtt tagtattcat gtctatggtg
    21961 accactttaa caacgtggaa tagccgtata ggagcttttt tcttacttat tttgctttta
    22021 ctacagttag catcaagtgc aggtacttat ccacttgctt tgacaaatga tttctttaga
    22081 tctattaatc cctggttacc aatgagctat tcagtttcgg gattacgaca aacaatctct
    22141 atcaacaagt cattttccta gctgtcatac tagctctatt tactggttta ggtatgctag
    22201 cctatcaaca taagaaaatg gaagaagatt aaaaaaatcg accgattaat gaactgcacc
    22261 ccaaaagtta gacagaaaaa atctaacttt tgaggtgttt tattatgaaa ttaa
//
LOCUS       DAVBBS010000034        22104 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVBBS010000034 DAVBBS010000000
VERSION     DAVBBS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22104
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.034"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..389)
                     /locus_tag="VQU32_001274"
     CDS             complement(<1..389)
                     /locus_tag="VQU32_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HET2472728.1"
                     /translation="MKTFFAKKRNIFLARLFLGQLPLLVSTYLFLSRQFLNFSVVFQF
                     LLVVINLASILVTVYLTREMRIREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITA
                     GESYQQIIAYIGAVLCLIIMLLLMWGL"
     gene            complement(386..550)
                     /locus_tag="VQU32_001275"
     CDS             complement(386..550)
                     /locus_tag="VQU32_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472729.1"
                     /translation="MKKKILIIFVLYLIMSIFLYPLRESAWYQLFYTIAYVIAVMIYF
                     AINKKKGEKK"
     gene            complement(849..968)
                     /locus_tag="VQU32_001276"
     CDS             complement(849..968)
                     /locus_tag="VQU32_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HET2472730.1"
                     /translation="MDFKSFIIGLVVGIFGPYMYNLIRKIFSKFPKKDTDSTP"
     gene            complement(971..1201)
                     /gene="blpU"
                     /locus_tag="VQU32_001277"
     CDS             complement(971..1201)
                     /gene="blpU"
                     /locus_tag="VQU32_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HET2472731.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAARGLQ
                     LGIKTGTWQGAATGAAGGAILGGVAYAATCWW"
     gene            complement(1270..1539)
                     /gene="blpJ"
                     /locus_tag="VQU32_001278"
     CDS             complement(1270..1539)
                     /gene="blpJ"
                     /locus_tag="VQU32_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HET2472732.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            complement(2006..2203)
                     /gene="blpI"
                     /locus_tag="VQU32_001279"
     CDS             complement(2006..2203)
                     /gene="blpI"
                     /locus_tag="VQU32_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HET2472733.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            2485..3072
                     /locus_tag="VQU32_001280"
     CDS             2485..3072
                     /locus_tag="VQU32_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HET2472734.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            3065..4654
                     /locus_tag="VQU32_001281"
                     /pseudo
     CDS             3065..4654
                     /locus_tag="VQU32_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
     gene            4665..6026
                     /locus_tag="VQU32_001282"
     CDS             4665..6026
                     /locus_tag="VQU32_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HET2472735.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMDESQKTAL
                     ETQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLANSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LIASQGQTQGTAEEPFLSQINQSIASLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLLLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     SIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            6083..6238
                     /gene="blpC"
                     /locus_tag="VQU32_001283"
     CDS             6083..6238
                     /gene="blpC"
                     /locus_tag="VQU32_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HET2472736.1"
                     /translation="MDKKQNLTSFQELTTTELNQIIGGGWWEDFLYRFNIIEQKNTKG
                     FHQPIQL"
     gene            complement(6282..7622)
                     /locus_tag="VQU32_001284"
     CDS             complement(6282..7622)
                     /locus_tag="VQU32_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2472737.1"
                     /translation="MNVAWILLYTLVTHGLKIVIFFKVDGISLTFERIFKAFLFKILL
                     AVVFGMLGYMVGNTYLSYFMEPLYGIGLSFLLLRELPKKLLLFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDDYSFIWLCIIIFNFFISLAFLKWLDYDFTSLRKGILDKDFQK
                     SLTQINWIMGAYYLVIQNLSYFEYEQGIQSTTVRHLILVFYLLFFMGIIKKLDTYLKD
                     KLHERLNQEQDLRYREMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EIYDSVLKDSSEKLQDNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTVVSILCDNAIEASVEACQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQDHVFRQVLTVIHIE"
     gene            complement(7636..8373)
                     /locus_tag="VQU32_001285"
     CDS             complement(7636..8373)
                     /locus_tag="VQU32_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2472738.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKEHHITPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANGQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSTRPHRVILYTKTDRLEFTASLEEVLKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(8378..8716)
                     /locus_tag="VQU32_001286"
     CDS             complement(8378..8716)
                     /locus_tag="VQU32_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2472739.1"
                     /translation="MKHMIIQTQKTVYKVNVDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQYGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            8993..9322
                     /locus_tag="VQU32_001287"
     CDS             8993..9322
                     /locus_tag="VQU32_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HET2472740.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(9490..10539)
                     /locus_tag="VQU32_001288"
     CDS             complement(9490..10539)
                     /locus_tag="VQU32_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2472741.1"
                     /translation="MKDLFLKRKQAFRKECLCYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEAIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(10536..11267)
                     /locus_tag="VQU32_001289"
     CDS             complement(10536..11267)
                     /locus_tag="VQU32_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2472742.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            11335..11745
                     /locus_tag="VQU32_001290"
     CDS             11335..11745
                     /locus_tag="VQU32_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET2472743.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMNATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            11755..12042
                     /locus_tag="VQU32_001291"
     CDS             11755..12042
                     /locus_tag="VQU32_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472744.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            12204..12443
                     /locus_tag="VQU32_001292"
     CDS             12204..12443
                     /locus_tag="VQU32_001292"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472745.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSSKHCF
                     EVVDRTDEVSNHTHDKPTLTWFEEIFEEYHSPFHN"
     gene            complement(12793..13929)
                     /gene="dnaJ"
                     /locus_tag="VQU32_001293"
     CDS             complement(12793..13929)
                     /gene="dnaJ"
                     /locus_tag="VQU32_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET2472746.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(14134..14487)
                     /locus_tag="VQU32_001294"
     CDS             complement(14134..14487)
                     /locus_tag="VQU32_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472747.1"
                     /translation="MSVNKNNLSDIADYHEKIKFFEELFNLDYNPTEMLVTVERNGNV
                     IYKEFTEVAVGKSKYDHTSVFKDVMIYFSDHICQELHLYKTQDPHYNKFEFDLAKRSL
                     KITTRDNTTIFISNE"
     gene            complement(14500..16323)
                     /gene="dnaK"
                     /locus_tag="VQU32_001295"
     CDS             complement(14500..16323)
                     /gene="dnaK"
                     /locus_tag="VQU32_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET2472748.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(16803..17327)
                     /gene="grpE"
                     /locus_tag="VQU32_001296"
     CDS             complement(16803..17327)
                     /gene="grpE"
                     /locus_tag="VQU32_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET2472749.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(17354..18388)
                     /gene="hrcA"
                     /locus_tag="VQU32_001297"
     CDS             complement(17354..18388)
                     /gene="hrcA"
                     /locus_tag="VQU32_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET2472750.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTGLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLEAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(18552..19061)
                     /locus_tag="VQU32_001298"
     CDS             complement(18552..19061)
                     /locus_tag="VQU32_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HET2472751.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTTTFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            19669..21606
                     /locus_tag="VQU32_001299"
                     /pseudo
     CDS             19669..21606
                     /locus_tag="VQU32_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            21604..22104
                     /locus_tag="VQU32_001300"
                     /pseudo
     CDS             21604..22104
                     /locus_tag="VQU32_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819352.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         6993 a         4580 c         3952 g         6579 t
ORIGIN      
        1 ttcaagcccc acatgagcag aagcatgatg attaagcaga gaacagcgcc aatataagcg
       61 attatttgtt ggtaggattc tcctgctgtg atacctctat acaaacaaat aatagacata
      121 aaacctgtca agccgatgaa cataagttga ttggttctag gactaaccaa atcatcatct
      181 tcaaactctc ttatcctcat ttccctagtg agataaacag taaccaaaat agaagccaag
      241 ttaataacta ctaaaagaaa ttggaaaact acggaaaaat ttaaaaactg acgagataga
      301 aatagataag tagaaacaag caagggcaac tgacctaaga acaatctcgc aaggaagatg
      361 ttccgttttt tagcaaaaaa agttttcatt tcttttctcc tttcttttta ttgatagcaa
      421 aatagatcat aactgcaatc acataggcta tggtataaaa tagctgatac caagcactct
      481 ccctaagcgg atatagaaag atggacatga ttagatacag aacgaaaata atcagtattt
      541 ttttcttcat aagatttcct cctaaatgtg cgatttatct tagttgagca agaacattta
      601 cactgctagt atagcactta ttttgacctt ggatcactca aatcataaat ggtcatcgaa
      661 acatcttgaa ttgttaaaaa atttaaaaag taagcattaa aaacatactt tcctctttat
      721 attgtattga taccaacttg tttgtagact tttcatcctg ctatcacata tcattttgac
      781 aggcgaaaca aaattaaaga aactcccctg taaattaagc tagcaaatac aggggagaaa
      841 tttatttttt agggggtact atctgtatcc tttttgggaa attttgaaaa tatttttcta
      901 attaaattat acatataagg accaaatata ccaactacta aaccaataat aaaactttta
      961 aaatccataa ttaccaccaa catgttgctg cataggctac acctccaagt atagctccac
     1021 ccgcagcacc agttgctgca ccttgccatg ttcctgtttt aatgcctagt tgaagacctc
     1081 ttgctgctgc tcctccaaca cctgctttgg caaaatctcc ccaattgcat ccgccacctt
     1141 caacgcaagc aagcatttca gtatccataa cagaaaattg tgacatcatt tttgtatcca
     1201 tgacaaatac tcctttttta aaaaactaaa ataaatcaga atagaatcct cataatttta
     1261 ctataagtct taccaactta gtcccaattt atcaccaacc atacctccta agcatgttaa
     1321 tccaccccca attgcaccaa tgtgtgctcc aacaaatgca ccagcaagtc cagctactcc
     1381 taaagtggcc aaacctgctc cagttccacc agttataatt cccgtagtga ctcctgtaat
     1441 cagtgcattt tgacaatcag tggagctata ccccccttca actttcgcaa gcatttcagt
     1501 atccataacc tctaactgtg acaacatttt tgtattcatg atgaatacct cctttttatt
     1561 ttcaatttgt taccaaagtc ttaaattcaa taaacaaata gattttttat agtatctttt
     1621 tgattttctt aaaaaagtat atacgtctac tatcttctta aaggtagcag tacctatttt
     1681 ttagtctaag atttcaataa tcttgagtat ctaaaatatc ttaatttcgt tattctcctt
     1741 gcaataaaaa gttttactat actatttatt aacttgcaga aagcaaaaaa tattagtaaa
     1801 taatagttta tagttaagtt ttttattcct accaatccat caactaagta aagcatcaac
     1861 gattacataa acgattgata atataattaa aattttgcta actatcttat tctcatcatt
     1921 cttagataac tttgatattt tgtaagtaag taaataagac agtaaattaa tagcgataat
     1981 aatactatat ttaagaatca taatcttaca aagaggacat aattcctgaa cctacacaaa
     2041 taagtgttgc tgctccccca gttatcggac cagtcgcagc agctaatagt actgctccaa
     2101 tacaaccacc gattgcagat cctaaattgc ctcttcctcc actaactatt tcgagttctt
     2161 cattatccat aacagaaaat tgttccatca tttttgtatt catgacaaat actccttttt
     2221 tcttttttta tttttgtctt gttgtaactt tgataagttt agtatatcat cgttttttaa
     2281 aatttttcat ccagatcttg aattgtcatc gaaacgtctt gaattagctt ttttatttca
     2341 agccacctct aaatgtttaa aaaaaataat ttctaatcac ttttttacca ttcaggaagt
     2401 tttaatgact attcaagatt tcataaaata tgaacttagt tttatgacat aatagaccta
     2461 tccactatat gaaaagaatt gccaatgact tcttataaac gtacatttgt tcctcaaata
     2521 gatgcgagag actgtggtgt cgctgcctta gcctcgattg ctaaattcta tggttcagat
     2581 ttttctctag ctcacttgag agaacttgca aagaccaata aagaagggac gactgctctt
     2641 ggcattgtaa aagccgctga tgaaatgggc tttgaaacaa gacctgttca agcagataaa
     2701 acgctctttg acatgagtga tgtcccctat ccatttatcg ttcacgttaa caaagaagga
     2761 aaactccaac attactatgt tgtctatcaa acaaagaaag actatctgat tattggtgat
     2821 cctgaccctt ctgtaaaaat cactaaaatg tcaaaagaac gctttttcta tgaatggact
     2881 ggagtagcta tttttctagc taccaaaccc agctatcaac cccataaaga taaaaagaat
     2941 ggtctactaa gcaagcttcc ttcctctgat tttcaaacaa aaatctctca ttgcttacat
     3001 tgttctctca agcttattgg tcactattat caatataggt ggttcttact atctccaagg
     3061 aatcttggat gaatacattc caaatcagat gaaatcaact ttaggaatca tctcagttgg
     3121 tctggttatc acctaggtta tcacctatat cctccaacaa gtcatgagct tctccagaga
     3181 ttatctccta accgttctga gccagagatt aagcattgat gtgattttat cctatattcg
     3241 ccatattttt gaacttccca tgtctttctt tgcgacacgt cgtacaggag aaatcatttc
     3301 acggttcaca gatgctaact ctattataga tgccttggct tctaccattc tttctctttt
     3361 tctggatgtt tctattctga ttcttgtagg gggagtcttg ctggcacaaa accctaatct
     3421 cttcctcctt tctcttcttt ccattcctat atacatgttc atcatctttt cttttatgaa
     3481 accttttgaa aaaatgaacc atgatgtcat gcaaagtaat tctatggtta gctctgccat
     3541 tatcgaagat atcaacggga ttgaaactat aaagtcgctc acgagtgaag aaaatcgcta
     3601 tcaaaatata gacagcgaat ttgtagatta tttggaaaaa tcctttaagc tcagtaaata
     3661 ttctatttta caaacgagtt taaagcaggg aacaaaatta gttctgaata tccttatcct
     3721 atggtttggc gctcaattag tcatgtcgag taaaatttct atcggtcagc tgattacctt
     3781 taacacactt ttttcttact ttacaactcc tatggaaaat attatcaacc tccaaaccaa
     3841 actcccatct gcgaaggtcg ctaataaccg tttgaacgaa gtctatctag tcgaatctga
     3901 atttcaagtt caagaaaacc ctgtccattc acattttttg atgggcgata ttgaatttga
     3961 tgacctttct tataagtatg gttttggacg agatacctta acagatatta atctcacgat
     4021 taaacaagga gataaggtta gcctagttgg agttagtggt tctggtaaaa caactttagc
     4081 caaaatgatt gtcaatttct ttgaacccta caaagggcat atttccatca atcatcagga
     4141 tattaaaaac attgataaaa aagtcttgcg ccgtcatatt aattacctac cccaacaagc
     4201 ctatatcttt aatggctcta tcttggaaaa tttaaccttg ggcggtaatc atatgattag
     4261 ccaagaagat attctaagag cttgtgaatt agctgaaatc cgtcaagaca ttgaaagaat
     4321 gcctatgggc tatcaaactc agctctctga tggagctggt ctatcaggag gacagaagca
     4381 acgaatagcc ctcgctcgtg ctcttttaac taaatctcct gttttaatac tagacgaagc
     4441 tactagcggt cttgatgtct tgactgagaa aaaagttata gataatctta tgtccctaac
     4501 tgataaaacc attctctttg tagcccatcg tctcagtata gccgaacgaa ctaaccgtgt
     4561 cattgttctt gaccagggga aaatcattga agttggtagt caccaagagt taatgcaggc
     4621 gcaaggcttc taccatcatc tgttcaataa ataaggagaa tgtcatgaat cctaatcttt
     4681 ttagaagcgt cgagttttat cagagacgtt accataacta tgcgacagtg ttaattatac
     4741 ctctttcatt actgtttact ttcatcttga ttttctccct tgttgccaca aaagaaatta
     4801 ctgttacttc ccaaggagaa atcgccccta caagtgtcat tgcatccatt cagtcaacca
     4861 gtgataatcc tatccttgct aatcatttag tggcaaatca agtagttgaa aaaggtgact
     4921 tactcatcaa atactctgaa acaatggatg agagtcagaa aactgcctta gaaactcaat
     4981 tacaaagact tgagaagcaa aaagaaggac ttggaatttt gaaacaaagt ttagaaaaag
     5041 cgactgatct tttttctggc gaggatgagt ttggctacca taataccttt atgaatttta
     5101 ctaaacaatc ccatgatatt gaactgggta tcacaaagac taacaccgaa gtttcaaatc
     5161 aagctaatct tgccaatagc agttcatcag ccatcgaaca agaaattaca aaagttcaac
     5221 aacaaattgg agaatatcaa gagttgagag atgctatcat aaataacaga gcacgcttac
     5281 caactggcaa tccgcaccag tcaattttaa atcgttatct tatagcctct caaggacaaa
     5341 cacaaggaac ggcagaggag ccatttttat ctcaaattaa tcaaagtatt gcaagtcttg
     5401 aatcatctat cgcaagcctc aaaattcagc aagctggtat cggaagtgta gcaacttatg
     5461 ataatagttt agcaaccaaa attgaagtac tccgcactca gtttttacaa acagcctcac
     5521 agcaacaact aaccgtggag aatcaattaa cagaattaaa agtacaacta gatcaagcga
     5581 cacagcgctt agaaaataat accttaaccg ctccaagtaa aggtatcgtt catctgaaca
     5641 gcgaatttga aggtaaaaat agaattccaa ctggtacaga aattgctcaa atattccctg
     5701 tcatcacaga tacaagagaa gtactaatca cttactacgt atcatctgac tatctcctct
     5761 tactagataa aggacaaact gtaagattaa aactggagaa gattggaaat cacggcacca
     5821 ccatcatcgg ccaacttcag agtattgatc aaactcctac cagaacagag caaggcaatc
     5881 tctttaaatt aaccgctctt gcaaaactat ctaacgagga tagtaaactc atccaatatg
     5941 gcttacaagg tcgcgtcact agtgtaacta caaagaaaac atattttgat tatttcaaag
     6001 ataaaatttt aacacattct gattaatttt cagataacac tctataacta tttattatct
     6061 tatcaaaaag gagaatcata acatggataa gaaacaaaac ctaacttcat ttcaagaact
     6121 aacaactacc gaactcaacc aaattatagg tggaggatgg tgggaagatt tcttatatag
     6181 atttaatata attgaacaaa aaaatacaaa aggatttcat cagccaatac aactataaaa
     6241 aaacaagacc aagagaacgc actctcggtc ttattttttc ttcattctat atgtattaca
     6301 gtaagtacct gacgaaaaac gtgatcttgg caggtggtat tgagactggt attgggatga
     6361 ctttccacaa ttttcataac ggtatagaga ccaactcctc tctcctcccc tttagaactt
     6421 gctccaaagg agaagatttc agaaatatcg attccctctt ctttgatgga attttcaatg
     6481 ataaaggtct cctgtgctcc attttttaaa aaggcaattg aaacatgagg ttgacaggct
     6541 tctacactag cttcaatagc gttgtcacaa aggatagaca caacggttag aaaatcaagt
     6601 agactcatcc cctctacctg aatctcctct ggaacttcga cattaaagac aatgttctta
     6661 tctctagctt ttagaaattt ccctgctagg agacttttga gggctttatc acgaatattc
     6721 accaatcgac caaggtcata tttattgtcc tgcaattttt cactagaatc ctttaagacc
     6781 gagtcgtaga tctcttttat ctgctccata tcctcctctt caatgcccag acgtaagcta
     6841 gtcaagaggt tggtgtagtc atggcgaaaa ctccgtactt ccttataaag ctcctctata
     6901 tgccgactat accgctccat ctctctgtag cgcaagtcct gctcttggtt cagtctctca
     6961 tggagtttgt ccttcaaata ggtatccaat ttcttgataa tccccataaa aaagagcagg
     7021 taaaagacta ggatgagatg gcgaacagtc gttgattgaa tcccctgttc atattcaaag
     7081 taagacagat tttgtatcac caaatagtaa gcccccatta tccagttaat ctgagtcagg
     7141 gacttttgaa aatctttatc gagaatcccc tttctcaagc tagtgaaatc atagtccaac
     7201 catttcaaaa aagctagaga aatgaagaaa ttgaaaatta ttatacataa ccaaataaat
     7261 gagtagtcat catatacttg cccttgtccc aaaaatggaa gcacaaaata ggaaacacct
     7321 ctataaaaga gattcactaa tatcattgga aagagaccat aaaagagaag gagtttttta
     7381 ggaagttctc ttaacaataa gaaagataag cctatgccgt acaagggttc cataaaataa
     7441 gatagataag tatttcctac catatagcca agcattccaa aaacaacggc caacagtatc
     7501 ttaaaaagaa aggccttaaa aatcctctca aaagtgagac taattccatc taccttaaag
     7561 aatatgacaa tttttaatcc gtgagtaaca agtgtataca acaatatcca agcaacattc
     7621 ataaactctc ctagctcagt gtaagttatt aatagcctca gacacttccc tgaccttata
     7681 acgcgcgatt agacaacttc caccattggg aaagaaaagc agtttttctt tcttatctaa
     7741 atgcaccaca ttggcaggat tgatgagaaa agagcgatgg cactgcaaga gacgaggctc
     7801 ctgcttgaga acctcctcta gactcgctgt aaattccagc ctgtctgtct tggtatagag
     7861 aataacacga tggggtcttg tggacgtttc gagatagtaa acctctttaa aaggatactg
     7921 gaattgagca aattttgatt taaagtaaaa gcaatcttcc gctagacttt tactgtcttg
     7981 accattggca tagaggaggg ctgtctcgat acgagattca aactcctctg ccgaaagggc
     8041 cttatcaata tagtccagag cagacacttg gtagcgaaag gacaggggca taaactccga
     8101 gtgagtcgtc acaaagacaa tcagggcata cggatcccga tcccgaatct ttctagccac
     8161 ttccagaccc ttcatctcct catttcgaat ctcaatatcc aaaaagaata gctgatgggc
     8221 ccccttctca tgcacctctg ccagcagttg gtctggcttg ccaaatacct caaaagagct
     8281 gggagtgata tgatgttcct tcaagagttt ctcaatcgtc gtttcaatcc tagtctgttg
     8341 ggaaaaatca tcctctaaaa caaatattct catcttctta ctctccctct ttcaaccatt
     8401 tttgacgaat ttctctatag cgacgtctgg catacttgac agcgtattga ccttcttctc
     8461 cgagaaaaag gattctttct tgaaaatcaa tcgattttaa tttatcaaga ttaaccaaac
     8521 aatttcgatg acatctcatc aaggtttccc catattggtt ctcaagatta cttaaatttt
     8581 gaagcatatt aaaactagct tcttctgtaa caatctgtac ggtatgggct ttagttggat
     8641 gtgtttggat atagtagata tcgtcgacgt ttactttata gactgttttc tgcgtctgaa
     8701 taatcatatg tttcattgga ttctcctcct aagaaaagaa taacagaaaa gttctagccc
     8761 aagcccaccc aatcctaaac agtctttgaa agctcctaaa aggattattt ttctacaaca
     8821 tcatgatgag cttacaacta ttttatcata aaattcactc agcgcaattc aagacatttc
     8881 aatgacaatt aaagatttga ataaaaaaaa agaattaaaa atgataaact aacagtatca
     8941 aaacacagtt gccagaagta agactggcac ccagattaaa ggaggcattc ccatgacaga
     9001 tacagacccc atcaaaagag ctcatacttt gattactgac ttaaacaaag cctaccaagc
     9061 atgcaaacag gcaagcgctg acgacgtccg ctttcaggag caattaaact ctattcttgg
     9121 ttttttagcc aaggctgaaa cagtggataa tcgattcttg attgaactag aaaaatttta
     9181 ccagacttcc agtcttctca tgggactcag cgctcttgat ccagatgctc caactcgcgc
     9241 cgcttggcgg gcctatgacc gcttccactt tgaccaagtc aagaccaagt taatactcaa
     9301 tgaaaatcaa agagcaaact aggaagctag ccgcaagctg tacttgagta cggtaaggcg
     9361 acgctgacgt ggtttgaaga gattttcgaa gagtataagt ctctacggac caaccattat
     9421 cctgtagtaa caaaaaagaa gctgagacag actgaactca acttcttttt tagtgtcgta
     9481 tcagcaatgt tagtcctgca tctgacgttt tacctgataa ggcaaataca agactagtaa
     9541 aaccaaacca gctcctagta aggctagaag ggccagtttt tcttggaagg taatcaaccc
     9601 aacaactaat tccacaagta aaacaaaact ggtcaatcct cgaactacct cctgtaagcc
     9661 tttttccttt tgccccttgt ccagcggaaa gagttgggtc aaatactggt agtcaaaggc
     9721 atgatagagg gccagcaact ggaagagcaa gaggtagtta aagagaacta ccactgctgt
     9781 cgcaatccaa gcttgctcga taaaaacctg cgccagtacg gaaagtaata gaagacgaag
     9841 actgagagca aagaggtcgc catttcgcag ataagaacgc agatagagat tttgccaaat
     9901 cttcccaggc accttctgaa cagcctttaa gataaagtcc agataggcac gacgcttaac
     9961 gctgtttgaa attcccttga cctgcgtaaa gagggcaaag aaacgaagca agacttgctt
    10021 acgcttgctt tcttgagaaa taacatagtc ccagtccagt ccagtttcag taaaaaattt
    10081 gctggccttt tgacgaaagt ggaaatattt tcctaccccc aataaaagca catatagcag
    10141 aaaaactggc aagccataac ccattgctaa aaataaaggc gcaaataaca gcaagaaaag
    10201 ggtctgtaca aagagccaaa agactaggga aatgccagtt tgacgcttga gatggagctt
    10261 gattgcctct tctccaacta agagaaagag cttgtctgga gcctccatat aggtggcaat
    10321 tcctccccaa agtaagagta aaacagacgt aattcctaca aacaaaagga taggccaatg
    10381 attttcagga aaatgttgta agagttgact gtactggtag gctagaaagc ccaacaggac
    10441 aagcaggaac aagacaaagt ggtcattgag cacatagcgc agataacaaa gacactcctt
    10501 acgaaaggcc tgctttctct ttaaaaacaa gtctttcata gatcctcctc tttggtcaga
    10561 gccaagtaaa tatcattcaa actagcctca ggcatatcaa aggcttcacg tagttgcagg
    10621 agattgcctt tggcacgcac ctctcccttg tgaagaatga caaaggcatc acacatcttt
    10681 tccgccgaat ccagcacgtg ggtactcatg agaatagact tgcccttttg cttctccact
    10741 tccaaaagct gaatcaaatc agaaatagcc agcggatcaa gaccaaggaa aggctcatcc
    10801 acgatgaaaa gacttggatc caccacaaaa gcacagataa tcatgacctt ctgcttcatc
    10861 ccttttgaaa aatgaacagg gaaccagtct aatttctgtt ccaaacggaa catttttaac
    10921 aagggctcta ctcgttcgaa agccactttt tgctcaatac cgtaagccat agcaaccgtt
    10981 tcgatatgct ctctgagggt caattcctca tacagactag gcgtctcagg aatgtagcca
    11041 atctgcttgc ggtagctagt cgcatctcct tgcagagtca ggccattgat attgatggag
    11101 ccactataag gtgccaacag accgataatc tcattgatcg tcgttgattt cccagcacca
    11161 ttgagaccaa tcaaaccgac caactgccca ctttcaacag taaaggacac atctttcaaa
    11221 acaggaacat gaacatagcc acctgtcagg tttttaattt ctaacatatt ttctccaaat
    11281 ctggtataat gtagctatat tatatcaaaa ttcagtacag tagaggtaga ttttatgtca
    11341 gattgcattt tttgtaaaat catcgcaggg gaaattcctg cttcaaaagt atatgaagat
    11401 gagcaggtcc ttgcctttct tgatatctct caagtaacac taggacacac cttggtcgtg
    11461 ccaaaagaac actatcgcaa tcttttggag atgaatgcta cgagcgccag ccaactcttt
    11521 gcccaagtac caaaagtagc tcaaaaagtc atgaaagtca ctaaggctgc tggtatgaat
    11581 atcatttcca actgtgaaga agtcgctggt caaacagttt ttcatactca cgttcacctt
    11641 gtgcctcgct acagtgctga cgatgacctc aagattgatt ttatcgccca cgaaccagac
    11701 tttgacaaac ttgctcaagt cgctgaaacc atcaaaaacg cttaaggagt tgctatgaaa
    11761 ttatccaacc tactgctatt tgcaggagct gcagccggaa gttatctggt tacaaaaaat
    11821 cgccaaacca tcacagatga agtcttgaat accactgacc gcgttcaagc tatcaaagac
    11881 gatgtggata ttatccaaaa tagcctgcaa atcatcgacc agcaaaaaga acttatcaag
    11941 gaataccaag aagacttgac ttacaagttt aaggtcttgg aaaaggatat ccagactaga
    12001 ctagctgtga taaaagaaat gcagggaact gaagataagt aaaaagagcc ccccggctct
    12061 tttttaaaac gtaaacaagt tatagtgaat tgaatttaga atagcacatc atagtttcta
    12121 aagcattgtt agagattact ttaaaatcct cttatcaatt tgttcatatt ctatttcaat
    12181 ctactatata tatcccatca actatgcatc ataatttagg taactcatac tcaataaaaa
    12241 tcaaaaagca aactagaaag ctaggcacag actgctcaaa acaccgtttt aaggttgtgg
    12301 atagaactga cgaagtcagc tcaaaacact gttttgaggt tgtggataga actgacgaag
    12361 tcagtaacca tacccatgac aagccgacgc tgacgtggtt tgaagaaatt tttgaagagt
    12421 atcattcacc atttcacaac taaatcgaag actttctgcc gtaatgaaaa cacctgaaac
    12481 agcttggttt cagctgtccg gaaactttaa gacttaggtt caaagtttag gtatggaact
    12541 tcgaaggagg tcgctaccgt ccgtcattac ttaggaaagt cttaaaaaat ctataaagta
    12601 aaaagagccc gacagctctt tttgtttata ctcaatgaaa atcaaaaagc aaactaggaa
    12661 actagccacg ggctgctcaa aacactgttt tgaggttgta gatacgactc acatatatac
    12721 ggcaagccga cgctgacgtg gtttgaagag attttcgacg ctgacgtggt ttgaagagat
    12781 tttcaaagag tattattctc catcaaaggc atctttaata tggtcaaaga agcctttttt
    12841 ctttggattt actttcaagt caccagcagc cgcgaattct ttcaaggcta ctttttggcg
    12901 gtcgttcaag cctgtcggtg ttacgacatt aacagtaacg tattggtcac caactgcacc
    12961 gccacgaagg ctcggtgccc ccttactacg taggcggaat ttcttaccag tctgagttcc
    13021 ctctggaata accaattcaa catcaccgtg aacagttgga atatctactg tatcaccaag
    13081 agccgcttgg acaaagttga ggttgagatt gtagaagata gtcgttcctt cacgttcaaa
    13141 cttgtcgcta gcttctacag aaactactac atacaagtcc ccataaggtc caccgttaaa
    13201 gcctgcttcg ccttgaccag cgaggcgaat ttgttgacct gtttccacac cagcagggat
    13261 tttcacatgt acgctatgag cttgtttctc atgacctgtt ccatgacagg ttgtacatgg
    13321 atatttgatt tcttttcctc gaccgtgaca gacatcacag gttacttggc gacgcatcat
    13381 accaagagga gtctgcgtat cgacgttaat gacaccagcg ccatgacagc gtccacaagt
    13441 gactggactt gtccctggct tagcaccaga gccattacat gtacgacagc cagcttcacg
    13501 atgatactta acttccttct cagttccgaa gatagcttct tcaaaggtca aattgacacg
    13561 atactggaga tcatctcctt ggcgaggagc gtttggattg cgcgaagaac cgcctccgcc
    13621 gaagaaactt gaaaaaatat cctcaaaacc accgaagcca cctgccccat tgaaaccacc
    13681 gaaaccacca gctccaccaa aaccaccatt ggcgcctgca gcaccatact ggtcataggc
    13741 agcacgtttt tggtcgtcac tcaaagtctc ataggcttct tgaacttcct tgtacttgtc
    13801 ctcagcacca ggctccttgt tgatatctgg gtgatatttt ttggaaagct tacgataagc
    13861 ctttttgatc tcgtctgccg aagcgttttt tgacaccccc agacgatcat aaaattcagt
    13921 attgttcata caagatacca agaccgtaaa attcgactga aaaataggaa atctgacgct
    13981 ggagcgacgc tcctagacag atttctcttt tttccgagaa tttaggtcgg gttcaattcc
    14041 tttcttttat attgatgaga taatactcca gccactcggc cctatccctt tttaccgaaa
    14101 aacagaggct gagttgcaac ctctgtggtt agattattca tttgaaataa aaatggttgt
    14161 attatctcta gtagtaatct tcaaagacct tttagctaaa tcaaactcaa atttattata
    14221 gtgtgggtct tgtgttttat ataggtgtaa ctcctgacat atatgatccg aaaagtaaat
    14281 cataacatct ttaaacacag aagtgtggtc atatttactc ttaccaacag ctacttctgt
    14341 aaattcttta taaatgacat ttccatttct ttccacagtc accagcattt ctgttggatt
    14401 ataatccaaa ttaaataatt cttcaaaaaa ctttatcttt tcatgataat ctgcaatgtc
    14461 agaaagatta tttttattaa cactcataac actagcccct tatttttcag taaactctcc
    14521 gtctacgacg tcatcgcctg cgtttcctgt tgcttgtgcg ccttctgctc cttcttgagc
    14581 ttgttgcgct gctgcagctt gttcgtagag tttaacagca agtccttgag ctttttcgtt
    14641 caatgcttca agttttgctt tcatgtcgtc caagttgttg tcttcttgag ctttcttaag
    14701 gtcatcaagg gcagcttggg cagcgtcacg ttctgcgtcg aagcctttac cttcagtttc
    14761 cttgattgtc ttttcagtcg caaagattgc ttggtctact tcattacgaa ggtctacttc
    14821 ttctttacgt ttcttatcgg cttcagcgtt tgcttctgca tctttcatca tgcggtcgat
    14881 ttcttcgtca gtcaaacctg agttcgattg gatgacaata gtttgttctt tttgagttcc
    14941 aaggtctttg gccttaacag acacgatacc gttcttgtcg atgtcaaatg ttacttcgat
    15001 ttgaggaatt ccacgaggtg cagctgggat atcagtcaat tggaagcgtc caagagtctt
    15061 gttatctgct gccattgggc gttcaccttg aagaacgtgg atatcaacgg ctggttggtt
    15121 gtctgctgct gttgagaaga cttgtgattt agatgttggg attgtagtgt tgcgatcgat
    15181 aagttttgta aatactccac ccattgtttc gataccaagt gacaatggcg ttacatcaag
    15241 aaggacaacg tccttgacat caccagtaat cacaccacct tggatagccg cacccatagc
    15301 aactacttca tcagggttta ctgatttgtt tggttcttta ccagtttcag ctttaacagc
    15361 ttcaacaacg gcagggatac gagttgaacc accaacaagg ataacttcgt cgatttctga
    15421 caagctcaaa cctgcatctg aaagggcttg acgaactgga acttttgtac gttcaacaag
    15481 gtcacgagtc aaatcgtcaa atttcgcacg agtcaaagtc atttccaagt gaagaggtcc
    15541 agcctcacct gcagtgataa atggcaagct gatttgtgtt gaagttacac cagaaaggtc
    15601 tttcttcgct ttttcagccg catctttcaa acgttgcatt gccatcttgt cagtagacaa
    15661 gtcgataccg ttttctttct tgaattctgc taccaagtgg tcaatgattt tttggtcaaa
    15721 gtcgtcacca ccaagtttgt tgtcccctgc agttgacaat acgtcgaaga caccgtcacc
    15781 caattcaagg atagagacgt cgaatgtacc accaccaagg tcaaatacca agattttttc
    15841 ttctttgtca gtcttgtcca aaccataagc aagagctgct gcagttggtt cgttaacaat
    15901 acgttctact tcaagaccag caattttacc agcgtctttt gttgcttgac gttgagcgtc
    15961 gttgaagtaa gccggaactg tgataacagc tttggttact ttctcaccaa ggtagtcttc
    16021 agcgtagcct ttcaagtatt gaaggatcat agctgagatt tcttgtggag tgtattcttt
    16081 tccatttgca gaaacttttt cagaagttcc catcttagat ttgatagaga taactgtatc
    16141 tgggtttgta actgcttgac gttttgcagc atcaccaacg atgatttctc cgtttttgaa
    16201 tgagactaca gatggagttg tgcggtttcc ttctgggttt gcgatgattt tgctttcagt
    16261 tccttcaaga actgcaactg ctgagtttgt tgtacctaag tcaataccga taattttaga
    16321 catgtgtttt tctccttgtt agttaatttt ctatttttct ttttgatact cttcttaagt
    16381 ggcgacgccg tcagagcttg acttcgtcaa tctctttgac taaactttga gcctaaggtc
    16441 tcaaaatttg cgcaaatagc accacggcga agagtatcgt ctttggttcg cttcgctcac
    16501 tattacaaag ccaaacatat tttatctttc ttcatagttt attgtcgttt cggacaagtt
    16561 ttctattatg taaattgcga cacgaggagt cgaaatcgat tttatttcga cgacgagtta
    16621 gtaaggaagc taggcaaacg tcatagcgat tgccgttttc tgacgagttg ctttagctac
    16681 cgtcagaatt gccgaatcga acacgcccta agctctgagt aaaaaagata gattttcttg
    16741 tgttcatcga acacttcgtc aatctcctat ttttactgcg agctttttac gagctttgta
    16801 tcttagttat acaccactac cattgctggg cgtaggatgc ggtcatggag tttgtagcct
    16861 ttttgaaaga cttgggcgat ggtatctact gggtgttcat cgtctgctgg gagagtttgg
    16921 atggccatat ggtagttatg gtcaaattcg ccatctgctg cgatttcttc aattccttct
    16981 tctttcaaag cgtgaatcaa gctttcttgc accatcccca agcccttctt cacatcatct
    17041 gtcaaacctt caactgcaag tgcacgctca aggttgtcaa gagatggtaa gattgctttt
    17101 gccaagtcct ggctacgata acgttgcaag ttttgacgtt cttcattggc acggcgttgg
    17161 atattttgca tttctgcatg agcgcgaaga tatttgtttt cgaactcatc tgcacgttca
    17221 tttgccaagt ccaactcaga cttttcagga gttgtttctt cagctgtttc cacaacttcc
    17281 tcttcttgaa cttcttctac ttcttcattt tttatatctt gggccatgtt cgcctccttt
    17341 aatgatttca atcttaatgt acttcgtaat gattactgct gaggtagcgg taaaaatctg
    17401 tcaacttcat ggtcaaaaca cggttgacca cattgacttg attgattagc tgttggtaat
    17461 ccagattaac tggaccgata atggctagaa ttccaactcc ccgataagga atgaggaact
    17521 tactactaat caccgctagg tcagctaaac aggactcttg accgtctgca acacgaacat
    17581 tttgcatctg atcctcacgc aacccctcac gaatctccaa ggccaccttt tgcggttggt
    17641 caaagaactg ataggcttct agattggcaa aattcaagag attgaccttg cccgccatca
    17701 caatgttttc gttgaacatt tccttaaaga tgtgttcaaa gagatcgatg acattatccg
    17761 ttgttgtaaa gtaacgctgg ataatctgcg gaatctccgt ccgaatcttg tagtgaatat
    17821 ctaaaacggt gtgaccgagg aaacgttcct gaatgatgct cttcagtttc agcaaatcct
    17881 cctgcaagaa gttccttgga atcagaaact gactagtaac cgttcgcgac tcgtctaggg
    17941 taaataccgc caaggctgta tgttgcccca aaacaacgat atcaaaggct gtcaaacgtt
    18001 gcctgctcgg ctcaacatcc agtgccacta ccgtacagcc actcaggcct gttagcaagt
    18061 tagcagcctc ttgcagaata tcctccaatt tgaagaattc ctgatcaaag gctttgacaa
    18121 tctcatatac ctcattttca gccagccggt gaaaatccag tgagtgtttc acatagtact
    18181 gaaaaccagc aacacttggc atccgaccgc ttgaagtatg agccttctca agcaaccctt
    18241 gtttttctag ttccgccatg tcattacgaa tggttgcact gctagagtta atagactctt
    18301 gcaaggcttt tgatccgaca ggttcgtgcg ttttggtaaa gatgtcaata atcagattta
    18361 aaatatcctg ctgacgctct gtaaccatct catcactcct ctctgtctga tcaattagca
    18421 ctcaatgcat ccaagtgcta acttatgatt ctattataca cccttaaaag agaatgtcaa
    18481 gacaaaaact caaaaaatta gcactctttt atcaagagtg ctaaaaatca atctgaagac
    18541 ctagaacctt gctattcatc tactcggtat ttccttttta gtctgaaata accatgaatt
    18601 agataagaaa tcagaaaagc atataaaaca aaaataagaa agaaagacat agaaagatcc
    18661 tctctaaaca aactcgtaca aacaacaaga ccacctaaga aagtagttgt acaggaacga
    18721 agtctagatc gcataacttc ccatttgagc cccttactca tatagacttg atcctctgga
    18781 agtaaatcag aagacgattt acgaagaatc gaacaagaac ctgctcctat caattcccaa
    18841 cctctatctc taaaatcttg cagttcatgc ttatattttt taagaaatct agaatcatag
    18901 atacggtaga tgacatcgtc tggttgacat tggtcaaaat agaacaaacc aaaacgactc
    18961 gttctatacc tccaaccttt caaatgcatc tcatgtaaat attcttcttc cttgtccaaa
    19021 tcaacaatgg tgaaaatccg aaattctact ctgctattca ttgtcttacc ccaaaattag
    19081 aaaacatgcc tggcgttatt tattagataa ttctttccac ttttgactca atctccaaaa
    19141 aatataagaa atctgaatcg caaaaactat caataaaacc caatctatta tgaaaatcaa
    19201 aaacactttc caactgaaag aactacctcc agtgacaaac tttgagcaaa acggtagtag
    19261 agctaaaaag agaagtaaaa taggaagcat ccgcattgtt aaaatccgtt tggcataaaa
    19321 aaatctttat ttaaactaaa atattatagc aaaatttacg ccagtttttg aacggctgat
    19381 gtagatattt tatactttca aaatgtttaa atgtgattat ttatttttga aaaatagctc
    19441 accagcccga ctgaaagtgc ttatagaatg ataataagtc gcctgccgaa aacagcggaa
    19501 aatagcggtg ttatgcggag ataatctgac gcgatgcgaa agtatattgc atacttattt
    19561 tcaacaattt agcagagtat ttttataagt gtgatataat agaagtataa tttgttctga
    19621 tagtttattt tatggagaag tagattttta gaatgcggag ggttcaatat ggttgagttt
    19681 ataaagtcta agaaagaaat gagtgaggag gatattaaag caaatttcat cactcctgct
    19741 attgtatcca aaggatggaa aaatggtgag catatcgctt acgaagaata cttcactgat
    19801 ggtcgaattg aggttagagg agataaggct cgtcgtaaag aaggaaaaaa atcagactat
    19861 tcactgtatt accaatttgg aactcgaatt gcaattgttg aggcaaagga taataaacac
    19921 agcgttcgag caggattaca acaagctatt gaatatggag agattttaga tgttccattt
    19981 gtttattctt cgaatggtga tggctttatt gaacacgacc gtatcacgag agaagaacgt
    20041 gagctggagt tagacgaatt ccctactcgt gaagaattat tttctcgtat gacgaaggaa
    20101 aaaggattga cgtacgaaat tacagaagct atctcaactc catactatac agacgccttc
    20161 tcaatgaaaa cgccacgcta ttatcagcaa atagctatca accgtactat tgaaacagtt
    20221 gccagaggac aaaaacgagt aatgtttgtg atggcaacag gaacagggaa aacgttcatg
    20281 gcttttcaaa ttattcatcg ccttcgaaaa gctggtttgg ctaaacgagt tttattctta
    20341 gcagatagaa acatcttagt agaccaaacg atggctgaag actttaagcc attcgaaaag
    20401 gtaatgacga aaattacacc aaaacttttg actgctcctg aaaaattaaa ttctttcgaa
    20461 atttatctag ggctttatca gcaactaact ggtgaagatg gaactgaaac acattatcaa
    20521 aaatttgaca aagacttctt tgatttaatc gtaattgatg aagcgcaccg tggttcagct
    20581 aaggaaaaca gtaactggcg taaggtaatt gattatttca gttctgcgac acagattggg
    20641 atgaccgcta ctcctaaaga aaccaagaat gcttccaata cggaatactt tggtaagcca
    20701 atctatactt atagtttaaa acagggaatc gaggatggtt ttttggctcc atatcgtgtt
    20761 atgagggtta atttagatgt ggatgtggat ggttatcgtc cagaaactgg aaaagttgat
    20821 gctaacggac aattaataga agataggtac tacggcagga aagattttga taaaaccatt
    20881 gtcattgatg atagaacgca aagagttgcc aagtttgttt ctgattatat gaagcaaaac
    20941 aatgcacgat ttgataaaac aattgttttt tgtgttgata ttgaccatgc cgagcgaatg
    21001 cgtgctgcac ttgtaaaaga gaatctagac ttagtccaag aagactatcg ttatgtcatg
    21061 caagtaactg gtgacaacgc tgaaggaaaa gctcaactgg ataactttat ggatgtcaat
    21121 tctaattttc ccgctattgt aacaacgtct aaattattaa cgacaggagt taatgctaaa
    21181 acatgtcgtt tgattgtttt agactctaat atccaatcca tgactgaatt taaacaaatt
    21241 attggtcgtg gcacacgtct ttatcctcaa aaggggaaag aattttttac gattattgat
    21301 tttcgaaatg ttaccaattt gtttgctgac cctgattttg atggtgagcc agtgaaggtg
    21361 ctggaaacag gtgcgaaaac agtcagtggt tctacgcctg gtttcgtagg tgaggaaggt
    21421 gacccagtag aaaaatatat cgttacagac aagcaggtta ccattcttaa ttctactgtt
    21481 caagtattgg atgaaaacgg gaaactgatt accgaaagcc tgaccgacta cactcgaaag
    21541 aatatcttag gtagctacgc cactttgaac gattttatca cagtttggca tacggcagat
    21601 aagaaacatt atgggaagtt tgctgatgga agcactcaag aaattgatgt tccttatgat
    21661 attcctgata cttgggagtg ggtgaggttt tctacattgg ttgaaattgt cagaggtggc
    21721 tctccacgac caatcaaaga ttatcttact tctgaagtag atggaataaa ttggataaaa
    21781 ataggtgata ctgaaaaggg tgaaaagtat ataaataatg ttaaagaaaa aatcaaaaaa
    21841 tcagggctta acaaaactag atttgtaaaa aaaggtacat ttttgttaac taattctatg
    21901 agttttggta gaccttatat tttgaatgtt gatggtgcaa tacacgatgg atggttggct
    21961 atttcgaact atgaaaactc attaaataaa gattacctat tctatattct ttcatcaaat
    22021 gtagtttatt ctcaatttct atctctaatt agtggagctg ttgtgaaaaa cttgaatagt
    22081 gataaagttg cttctattct tatc
//
LOCUS       DAVBBS010000035        21460 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVBBS010000035 DAVBBS010000000
VERSION     DAVBBS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21460
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.035"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            66..287
                     /locus_tag="VQU32_001301"
     CDS             66..287
                     /locus_tag="VQU32_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2472752.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            804..1043
                     /locus_tag="VQU32_001302"
                     /pseudo
     CDS             804..1043
                     /locus_tag="VQU32_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1187..2416
                     /locus_tag="VQU32_001303"
     CDS             1187..2416
                     /locus_tag="VQU32_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET2472753.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            2436..5132
                     /gene="ppc"
                     /locus_tag="VQU32_001304"
     CDS             2436..5132
                     /gene="ppc"
                     /locus_tag="VQU32_001304"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET2472754.1"
                     /translation="MSLQKLENSSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATNLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYEIILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    5215..5432
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5545..6549
                     /locus_tag="VQU32_001305"
     CDS             5545..6549
                     /locus_tag="VQU32_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2472755.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     IELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTIKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            6618..7484
                     /locus_tag="VQU32_001306"
     CDS             6618..7484
                     /locus_tag="VQU32_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2472756.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            7481..8239
                     /locus_tag="VQU32_001307"
     CDS             7481..8239
                     /locus_tag="VQU32_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2472757.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            8329..10089
                     /gene="dnaG"
                     /locus_tag="VQU32_001308"
     CDS             8329..10089
                     /gene="dnaG"
                     /locus_tag="VQU32_001308"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET2472758.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            10092..11201
                     /gene="rpoD"
                     /locus_tag="VQU32_001309"
     CDS             10092..11201
                     /gene="rpoD"
                     /locus_tag="VQU32_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET2472759.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            11215..11544
                     /locus_tag="VQU32_001310"
     CDS             11215..11544
                     /locus_tag="VQU32_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET2472760.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            11659..12702
                     /gene="cpoA"
                     /locus_tag="VQU32_001311"
     CDS             11659..12702
                     /gene="cpoA"
                     /locus_tag="VQU32_001311"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HET2472761.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            12718..14043
                     /locus_tag="VQU32_001312"
     CDS             12718..14043
                     /locus_tag="VQU32_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2472762.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGILIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGALYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFRTVLYLPQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            14074..14250
                     /locus_tag="VQU32_001313"
     CDS             14074..14250
                     /locus_tag="VQU32_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET2472763.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            14309..14443
                     /locus_tag="VQU32_001314"
     CDS             14309..14443
                     /locus_tag="VQU32_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HET2472764.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            14507..15817
                     /gene="obgE"
                     /locus_tag="VQU32_001315"
     CDS             14507..15817
                     /gene="obgE"
                     /locus_tag="VQU32_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET2472765.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            15827..15985
                     /locus_tag="VQU32_001316"
     CDS             15827..15985
                     /locus_tag="VQU32_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472766.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            17035..18294
                     /locus_tag="VQU32_001317"
     CDS             17035..18294
                     /locus_tag="VQU32_001317"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET2472767.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            18310..18861
                     /locus_tag="VQU32_001318"
     CDS             18310..18861
                     /locus_tag="VQU32_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2472768.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            18854..20131
                     /locus_tag="VQU32_001319"
     CDS             18854..20131
                     /locus_tag="VQU32_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2472769.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIDRLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            20147..21007
                     /locus_tag="VQU32_001320"
     CDS             20147..21007
                     /locus_tag="VQU32_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HET2472770.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            21230..21340
                     /locus_tag="VQU32_001321"
                     /pseudo
     CDS             21230..21340
                     /locus_tag="VQU32_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818216.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase"
BASE COUNT         6560 a         3687 c         4816 g         6397 t
ORIGIN      
        1 ccagttagga cactttgaat gatttcttag ttttatatat tatacttttc tcaggagaaa
       61 gctagatgta cagacgtttg agagatttga gggaagatca cgatttgacc caaaaacaaa
      121 tagctaaaat actttcgttt acaaactcag cttatgctaa aattgaacgg ggtgagcata
      181 cgttaacagc agatgtattg gtaactctct caaactttta cgatgtcagt acagactacc
      241 tattggaatt gacagatttt cctgataaaa ttcgctttag aaaataatct cctcaatttc
      301 atagagtgtg aaaatgagga gattttttat ttgttctttg aaaattaaat agtccaaaat
      361 ggtactttcc tcatttgtgg agcaaatttg aatggctcgc catgataaga gcgattttaa
      421 agccatcaat aaaatagagc gatactttat atgccatgat acaaatgata tacaatgata
      481 cttctgaccg ttcaggctgc catcgtaaaa gagcagcaag tgaaattctt atgatgactt
      541 catcagtcat gccacgttga atgtgtgagt ttgttagaga aacgcaatta atcctcaaaa
      601 ggttccccga accttttgag ttccacagac gcatcacgtg gagtgtgtaa gcttgttgct
      661 aaaagcgtaa aaaccttgga acgaaaggaa taatagactt cctgcgaaac aaaaatacaa
      721 tacaataaaa ctatggatga tgaagcaagt aaacaattaa gcgatagccg tttcaagagc
      781 cttgtaggtg ttcagcgcac gagatgttag ctgtgttaaa aacagcttat caacgcaacg
      841 taaacgcgca aaaggtggac gaaaaaccaa attaagccta gacgatctcc tcatggtaac
      901 tattcaatac atgcgagaat agcgcactta tgaacaaatt gcggctgatt ttggcattca
      961 cgaaagcaac ataattagtc ggaggtcaat ggattgaagc aactcttatt caaaatggtt
     1021 ttacgatttc aaattctgcc ttaattctgt aaaaacagta aaattcgaag gattgtaagg
     1081 taagagtttt tttctttctg aaaaaatggt ataatagcaa tcaaaactag aaaataaaac
     1141 ggaatttgga acagatttgt ctgtatccta gtagagtggt gatactatga agattagtaa
     1201 gaggcactta ttaaattatt ccatcttgat tccctacttg cttttatcta ttttgggctt
     1261 gattgtggtc tattcgacca ccagtgctat tttaattgaa gaaggcaaga gcgccttgca
     1321 gttggttcga aaccaaggaa tcttttggat tgttagtttg atactgattg ccttaattta
     1381 taaattgaga ctagattttt tgagaaatga gcgactaatc attttagtta tattaataga
     1441 aatgctttta ttgttcttgg ctcgttttat tggtatttca gtaaacgggg catacggttg
     1501 gatttcggtt gcaggagtaa ctattcagcc agctgagtac ttaaaaatca ttattatttg
     1561 gtatttagct caccgattct ccaaacagca agaagaaata gctacttatg attttcaagt
     1621 tttgactcaa aatcaatggc ttccccgtgc ttttaatgat tggcgattcg ttctcctagt
     1681 tctgattgga agtttgggaa ttttccctga tttaggaaat gcgactattt tagtcttggt
     1741 ttccttgatt atgtatacag ttagtggaat cgcttatcgc tggttttcaa ccattctggc
     1801 gctcgtatct gccgcttctg tctttgtctt gaccactatc agcctaatcg gtgttgagac
     1861 cttttcaaaa attccagtat ttggctatgt agccaaacgc tttagtgcct tttttaatcc
     1921 ttttgccgat cgtgctgatg caggtcacca gttagctaat tcttattttg ccatggtcaa
     1981 tggtggttgg tttggtctag gtcttggaaa ctcgattgaa aaacgaggtt atttgccaga
     2041 agctcataca gactttgtct tttctatcgt gattgaagaa tttggctttg ttggtgccag
     2101 tcttatttta gctctcttgt ttttcatgat tttgcggatt atcttggtcg gtattcgagc
     2161 ggagaatcct ttcaatgcca tggttgcact cggtgtcgga gggatgatgt tggttcaggt
     2221 atttgtcaat atcggaggga tttcgggctt gattccatct acaggagtaa ccttcccctt
     2281 cttatcccag ggtggaaata gtcttctagt cttatcagtg gcagtagcct ttgtcttaaa
     2341 tattgatgcc agtgaaaaac gcgctaagtt gtaccgagaa ttggaaaatc aaccaatgaa
     2401 ccttctgttg aagtaggata aagaaaggat agtttatgtc tcttcaaaaa ttagaaaatt
     2461 ctagtaataa aagtgttgtg caagaagaag tcttgattct aacagaatta ctggaagata
     2521 ttactaaaaa tatgcttgcc ccagagacct ttgaaaaaat aatacagttg aaagaattat
     2581 caacgcagga agattatcaa ggtctaaacc gtctagtgac tagcttatca aatgatgaaa
     2641 tggtctatat ttcacgctat ttctctatct tgcctctttt gattaatatt tcagaggatg
     2701 tggatttagc ttatgaaatc aatcatcaaa ataatattga tcaggactat ttaggtaaat
     2761 tatctacaac gattaaattg gtagcagaaa aggaaaatgc cgttgagatc ctagaacact
     2821 tgaatgttgt tccggttttg acagcccatc caacacaagt gcaacgcaaa agtatgttgg
     2881 atttaacaaa tcatattcat agtcttttgc gtaaataccg tgatgttaag ttggggttga
     2941 tcaataaaga taaatggtac aatgatttgc gtcgttacat cgaaattatc atgcagacag
     3001 acatgattcg tgagaaaaaa ttaaaagtga ctaacgaaat cacgaatgct atggaatatt
     3061 ataacagctc ctttttgaaa gctgtacctc atttgacgac ggagtataag cgcttagcgc
     3121 aagcgcatgg tctgaattta aaacaggcta aaccaatcac catgggtatg tggataggtg
     3181 gtgaccgtga tggaaatcca tttgttacag caaagacctt gaagcagtct gcactcactc
     3241 aatgtgaagt catcatgaac tactatgata aaaagattta ccaactttat cgtgaatttt
     3301 ctctttcaac tagcattgtc aacgtcagca agcaagtcag agaaatggct cgtcaatcca
     3361 aggataactc gatttaccgc gaaaaagagc tttaccgtcg tgccttgttt gatattcaat
     3421 caaaaattca ggcaactaaa acctatctga ttgaggatga agaagttggg actcgttatg
     3481 aaaccgccaa tgatttctac aaggatttga ttgccattcg agattctcta ctagagaata
     3541 agggcgagtc cttgatttca ggtgattttg tggaattatt gcaggcagta gagatatttg
     3601 gtttttacct agcatcaatt gatatgcgac aagactctag cgtctatgaa gcctgtgtgg
     3661 cagaactctt gaaatcagca ggaattcatt ctcgttatag cgagttgagc gaagaagaaa
     3721 agtgtgacct tcttttgaaa gaattagaag aagatccccg aattctttct gcgactcacg
     3781 cagaaaaatc agaattatta gcaaaagaac tagctatttt taagacggct cgtgttttga
     3841 aagataagtt gggagatgat gtcatccgtc agaccatcat ttcacatgca accaaccttt
     3901 ctgatatgct agaattagct attctgttaa aagaagtagg actggtggat acggaaaggg
     3961 cgcgtgttca gattgttccc ctttttgaaa caattgaaga cttggatcat tcagaggaaa
     4021 caatgagaaa atatctttct cttagccttg ccaaaaaatg gattgactca cgaaataact
     4081 accaagaaat catgcttggc tactctgaca gtaataaaga tggcggttac ttgtcatcat
     4141 gttggaccct ctacaaggct caacaacaat tgactgctat tggagatgaa tttggcgtta
     4201 aggttacctt cttccatggt cgtggtggta ctgtcggtcg tggtggtggg ccaacctatg
     4261 aagccattac atctcaaccg ctcaagtcta tcaaggatcg tatccgtttg acggagcagg
     4321 gtgaagtaat tgggaataaa tacggtaaca aagacgccgc ttactataac cttgaaatgc
     4381 tagtatcggc agctattaac cgtatgatta ctcagaagaa gagcgatacc aataccccaa
     4441 atcgttatga agccattatg gatcaagtag tggaccgtag ttacgatatc taccgtgatt
     4501 tggtctttgg taatgagcat ttctatgatt atttcttcga gtcaagtcca atcaaggcta
     4561 tttcaagttt taatattggt tctcgtccag ccgctcgtaa gactattact gaaatcggtg
     4621 gtttgcgtgc catcccttgg gtattctcat ggtcacagag tcgtgttatg ttccctggat
     4681 ggtacggggt tggttcaagc ttcaaggaat ttatcaataa aaatccagag aatattgcta
     4741 tcttacgaga tatgtaccaa aattggcctt tcttccaatc gcttctttca aatgttgata
     4801 tggttttgtc aaaatcaaat atgaatattg cttttgaata tgctaaactt tgtgaagacg
     4861 agcaagttaa ggccatctat gagattattt taaatgaatg gcaagttact aagaacgtta
     4921 tcttggctat tgaaggacat gacgaactct tagctgacaa tccatatcta aaagctagtc
     4981 tggattaccg tatgccttac tttaatattc tcaactatat tcagttagag ttgattaaac
     5041 gccaacgtcg tggagaattg tccagtgatc aagaacgatt gattcatatc accatcaacg
     5101 gaattgcgac aggattgcgt aattcaggtt gataattttc aagagtgaat gctaaaagtg
     5161 aatatcaaaa aaattctaat agactattga caagtagttt aaaaatgata taatttaacc
     5221 attcagaaaa gtaatcatac aaacttttta gagagtctgt ggtagctgaa aacagataag
     5281 tggcgatgat gaaaattggg ctgaatgcta tttagaattt gaaattataa aaattcggta
     5341 agcacacctt acagtgcatc tcgttattgc gagactgagc gatagggaaa ttccctataa
     5401 ttgaggtggt accgcgcatc gacgtcctca cacaagtttt ttgtgtgagg atttttttga
     5461 tggaggttag tatggaaaga aaacgatggc gtcgcttgtt tagataagtg aaatatgtta
     5521 aaggaaataa aaaggagaaa cagaatgaaa aataaacgtt taattggaat tattgctgca
     5581 ttagcagtct tagtagcagg aagcttgatt tattcttcaa tgaataaatc agaagctcag
     5641 aataataagg atgagaagaa aataaccaag attggtgtgc ttcaatttgt gagccatcca
     5701 tcccttgatt tgatttataa agggatccaa gatggacttg cagaagaagg atataaagat
     5761 gatcaagtta aaattgattt tatgaactca gaaggtgatc aaagtaaggt tgcgacaatg
     5821 agtaaacaat tggttgcaaa tgggaatgac cttgtggttg gtattgcaac accagcagcc
     5881 caagggttgg ctagtgcaac aaaagaccta ccagttatca tggccgctat tacagaccca
     5941 attggtgcta acttggttaa agatttgaaa aaaccaggtg gtaacgttac aggggtatct
     6001 gaccacaatc cagctcaaca acaaattgaa ctcatcaagg ctctgacacc gaatgtgaaa
     6061 acaatcggag ctctttactc aagtagcgaa gacaattcaa aaacacaggt tgaagaattt
     6121 aaggcttatg ctgaaaaagc aggtctgaca gtggaaacat ttgcagttcc ttcaacaaat
     6181 gaaattgcct caactgtcac tgttatgact atcaaggtag atgctatttg ggttccaatt
     6241 gataacacca ttgcatcagg atttccaacg gttgtctcta gcaatcaaag ttctaagaaa
     6301 ccaatttatc ccagtgcgac agctatggta gaagtaggtg gtttggcatc agttgtaatt
     6361 gaccaacatg accttggtgt ggcaacaggt aaaatgattg tgcaagtctt gaaaggtgca
     6421 aaaccagccg ataccccagt caatgtcttt tcaactggta agtcagtcat caataaaaaa
     6481 atagcacaag aactaggtat tactattcct gagtctgttc tcaaagaagc aggacaagtc
     6541 atcgaataaa taaggtaggg aggaggagac tcttcccatt ttttaaaaag atgaatatag
     6601 aaaggattaa ataaaatatg attgtttcca ttatttctca aggatttgtc tgggctattc
     6661 taggtctggg aatctttatg acatttagga ttttaaactt tccagatatg acgacagaag
     6721 gttccttccc tcttggggga gctgttgctg tcactttgat aaccaaaggc gtgaacccat
     6781 ttttagcgac acttgttgct gtaggagcag gttgtttggc tggaatggca gcaggccttc
     6841 tttatacaaa agggaagatc ccaaccttgc tctcagggat tttggtgatg acttcttgtc
     6901 actcaatcat gctcttgatt atgggacgtg cgaatttagg cctgcttgga accaagcaaa
     6961 ttcaggatgt tttgcctttt gattcggatt tgaatcaact cttgacaggt ctcatctttg
     7021 tgagcattgt tattgctctc atgctctttt tcttggacac taaactcgga caagcctata
     7081 ttgctacagg ggataatcct gatatggcta gaagtttcgg gattcatact ggacgcatgg
     7141 agctcatggg cttggtctta tcaaatggtg tgattgccct tgcaggtgcc ctcattgctc
     7201 agcaagaagg ctatgccgat gtgtctcgag ggatcggggt tatcgttgtg gggcttgcaa
     7261 gtttgattat tggagaagtt attttcaaga gtttgagctt ggcagagcgt ttggttacta
     7321 tcgttgtagg ttctatcgct tatcaatttt tagtgtgggc agttatcgca cttggcttta
     7381 atacaagtta ccttcgttta tacagtgcct tgattttagc agtctgcctc atgattccaa
     7441 catttaagca aacaatcttg aaaggagcca agttaagcaa atgacagcaa ttgtagaatt
     7501 aaaaaatgca accaaaattg ttaaaaatgg ctttgatgaa gaaaagatta ttttaaatga
     7561 tgtttcctta gaaatttttg aacgggactt tatcacgatt ttgggcggaa atggtgctgg
     7621 aaaatcaacc ctctttaaca ctatagcagg gaccttatca ctaactagtg gaactatccg
     7681 tattttaggt gaagatctca ctaagttttc acccgagaag cgtgccaagt acctgtctcg
     7741 tgtcttccaa gatccaaaga tggggacagc tccccgtatg acggtcgctg aaaatctttt
     7801 aatcgccaag tttcgtggtg agaagcgtgg attgttacca cgacgcttga ctagctataa
     7861 ggatgaattt caggcaacca ttgaaaaagt aggaaatggt cttgagaaac acttgaatac
     7921 accgattgag ttcttatcag gtggacaaag acaggctttg agtctcttga tggcaacctt
     7981 gaagcgacct gaattactcc tgttagatga gcatactgct gccctggatc caaagactag
     8041 tgttgctttg atggaattga cagatgaatt tgttaagaaa gatcagctaa cagcccttat
     8101 gattactcat catatggaag atgctctcaa atacggcaat cgcttgattg tcatgaaaga
     8161 aggacggatt atccaagatt taaaccaaga agaaaaagca aaaatgaaaa tctctgatta
     8221 ttatcaactc tttgaataaa gtagggaaaa tgagtgaaat ggtttacttt ttttctgaaa
     8281 taaagtatac tatataaagt aaactatgat aacatggagg tattgtgtat ggttgacaaa
     8341 caagtcattg aagaaatcaa aaacaatgcc aacattgtgg aagtcatagg agatgtgatt
     8401 tctttacaaa aggcaggacg gaactatcta gggctctgtc cttttcatgg tgaaaaaaca
     8461 ccttctttca acgttgtaga ggacaagcag ttttaccact gttttggttg tggtcgctca
     8521 ggtgatgtct ttaaattcat cgaggagtac caaggggtta cctttatgga ggctgtccaa
     8581 atcttaggtc agcgtgtcgg gattgaggtt gaaaaaccgc tttatagtga acagaagcca
     8641 gcctcgcctc accaagctct ttatgatatg cacgaagatg cggctaaatt ttaccatgct
     8701 attctcatga caacgactat gggcgaagag gccagaaatt acctttatca gcggggtttg
     8761 acagatgaag tgcttaaaca tttttggatt ggtttagcac ctccagaacg aaactatctc
     8821 tatcaacgtt tgtctgatca gtatcgtgaa gaggatttac tggattcagg cctgttttat
     8881 ctctcggatg ccaatcaatt tgtagacacc tttcacaatc gcattatgtt tcccctgaca
     8941 aatgaccaag gaaaggtcat tgccttctca ggtcgtatct ggcaaaaaac ggattcacaa
     9001 acttctaagt ataaaaacag ccgatcgact gcaattttta acaaaagtta cgaattatat
     9061 catatggata gggcaaaaaa atcttctgga aaaactagtg agatttacct gatggaagga
     9121 ttcatggatg ttattgcagc ctatcgggct ggaatcgaaa atgctgtggc gtcgatggga
     9181 acggccttga gtcgagagca tgttgagcat ctgaaaaggt taaccaagaa attggttctt
     9241 gtttacgatg gagataaggc tgggcaagcc gcgatattga aagcattgga tgaaattggt
     9301 gatatgcctg tgcaaatcgt tagcatgcct gataacttgg atcctgatga gtatctacaa
     9361 aaaaatggtc cagaagactt ggcctatcta ttaacaaaaa ctcgtattag tccgattgag
     9421 ttctacattc atcagtacaa acctgaaaac agtgaaaatc tgcaggctca gattgagttt
     9481 attgaaaaaa tagctccctt gattgttcaa gaaaagtcca tcgctgctca aaacagctat
     9541 attcatattt tagctgacag tctggcgtcc tttgattata cccagattga gcagattgtt
     9601 aatgagagtc gtcaggtgca aaggcagaat cgcatggaaa gaatttccag accgacgcca
     9661 atcaccatgc ctgtcaccaa gcagttatcg gctattatga gggcagaagc ccatctactc
     9721 tatcggatga tggaatcccc tcttgttttg aacgattacc gtttgcgaga agactttgca
     9781 tttgctacac ctgaatttca ggtcttatat gacttgcttg gccagtatgg aaatcttcct
     9841 ccagaagttt tagcagagca gacagaggaa gttgaaagag cttggtacca agttttagct
     9901 caggatttgc ctgctgagat atcgccgcag gaacttagtg aagtagagat gactcgaaac
     9961 aaggctctct tgaatcagga caatatgaga atcaaaaaga aggtgcagga agctagccat
    10021 gtaggagata cagatacagc cctagaagaa ttggaacgtt taatttccca aaagagaaga
    10081 atggagtaat aatggcaaca aaacaaaaag aagtaacaac atttgacgta caggtagcag
    10141 aatttatccg taatcataag caaaaaggga cagcaacaga tgatgaaatc aatgcaagtc
    10201 tggttattcc ttttaccttg gacgctgatg ggattgaaga tctcttgcaa cggattcagg
    10261 atgcagggat ttctatcaca gataacgaag gaaatccaag tgcgcgtgtt cttagcaatg
    10321 aagaagaacc agaactcagc gatgaggact tgattggatc aacttctgct aaggtcaatg
    10381 accctgtccg tatgtacttg aaagaaatag gggtcgttcc tctcttgacc aatgaagagg
    10441 agaaagagtt ggcactggct gttgaagctg gcgatatcga agccaaacaa cgtcttgcgg
    10501 aagccaatct tcgtttggtt gtttccattg ccaaacgcta tgtcggtcgt ggcatgcagt
    10561 tccttgactt gattcaagaa ggaaatatgg gcttgatgaa ggcggttgac aagtttgact
    10621 attctaaagg gttcaagttt tcaacttatg caacttggtg gattcgtcag gctatcactc
    10681 gtgctattgc ggaccaagct cgtaccatcc gtatcccagt tcacatggtt gaaactatca
    10741 ataaattggt tcgtgaacag cggaatctcc ttcaagaatt ggggcaagat ccgacaccag
    10801 aacagattgc tgaacgaatg gatatgacac ctgataaggt tcgtgaaatc ttgaagattg
    10861 cccaagaacc agtatctctt gaaactccta tcggtgaaga ggacgatagc caccttggag
    10921 actttatcga agatgaagtg attgaaaatc cagtggatta tacgactcgt atcgtcttgc
    10981 gtgagcaatt ggatgaaatc ttagatactc ttacagaccg tgaagaaaat gttctgcgtc
    11041 tacgttttgg actagatgat ggaaaaatgc gcacacttga agatgtgggg aaagtcttta
    11101 acgtaactcg tgagcgtatc cgtcagattg aagcaaaggc tttgagaaaa ctacgccaac
    11161 caagtcgcag caaaccgctt cgtgatttta ttgaagacta agagtgagga aaatatggct
    11221 tatacagaag agcaaattga aaacatcaaa acacggattt taacagcctt ggaagaagtc
    11281 atcgaccctg agttgggaat cgatattgtc aatcttggtt tgatctatga gattcgtttt
    11341 gacggtgaca cagggcaaac agagattgac atgactttga caactatggg ttgtccctta
    11401 gcagaccttt tgacagacca gatttatgat gctatgatag aggttccaga agtaacggat
    11461 actgaggtta aattagtctg gtatccagcc tggacagttg aaaaaatgag tcgctatgcc
    11521 cgcattgccc taggcattaa gtaaacatat aaaagcatgt gagagactag aggaaaagcg
    11581 aggaaatttc ccctcttttc ctctagtctc tcctttcttt tgctgatttt attcaaagaa
    11641 aatgatataa tagtagttat ggagaaaaag aaattacgca tcaatatgtt gagttcaagt
    11701 gagaaagtag caggacaggg agtttcaggt gcttaccgtg aattagttcg tcttcttcac
    11761 cgtgctgcca aggaccaatt gattgttaca gaaaatcttc caatcgaggc agatgtgact
    11821 cactttcata cgattgattt tccctattat ttatcaacct tccaaaagaa acgctcaggg
    11881 agaaagattg gctatgtgca tttcttgcca gctacacttg aaggaagttt gaaaattcca
    11941 tttttcttaa agggaattgt gaaacgctat gtattttctt tttacaaccg gatggagcac
    12001 ttggttgtgg tcaatcctat gtttattgag gatttggtag cagctggtat tccacgtgaa
    12061 aaagtgacct atattcctaa ctttgtcaac aaggaaaaat ggcatcctct accacaagaa
    12121 gaggtagtca gactgcgcac agatcttggt cttagtgaca atcagtttat cgtagtaggt
    12181 gctgggcaag ttcagaaacg taaagggatt gatgacttta tccgtctggc tgaggaattg
    12241 cctcagatta cctttatctg ggctggtggc ttctcttttg gtggtatgac agatggttat
    12301 gaacactata agaaaattat ggaaaatccc cctaaaaatt tgatttttcc aggcattgta
    12361 tcgccagagc ggatgcgcga attgtatgct ctagcggatc ttttcttgtt gcctagttac
    12421 aatgagctct ttcctatgac tattttagaa gctgcgagtt gtgaggctcc tattatgttg
    12481 cgtgatttag atctctataa ggtgattttg gagggaaatt atcgggcgac agcgggtaga
    12541 gaagagatga aagaggctat tttggaatat caagcaaatc ctgctgtctt aaaagatctc
    12601 aaagaaaagg ctaagaatat ttccagagag tattctgaag agcatctgtt acaaatctgg
    12661 ttggactttt atgagaaaca agccgcttta gggagaaagt aaaaagtgag gtaatctatg
    12721 cgaattggtt tatttacaga tacctatttt cctcaggttt ctggtgttgc gaccagtatt
    12781 cgaaccttga aaacagaact tgaaaagcag ggacatgctg tttttatctt tacgacgaca
    12841 gataaggatg tcaatcgcta cgaagattgg caaattatcc gcattccaag tgttcctttc
    12901 tttgctttta aggatcgtcg ctttgcctac cgaggtttta gcaaggcact tgaaattgct
    12961 aaacagtatc agctagatat tatccatact cagacagaat tttctcttgg cctgttgggg
    13021 atttggattg cgcgtgaatt gaaaattcca gtcatccata cctatcacac ccagtatgaa
    13081 gactatgtcc attatattgc taaggggata ttgatccggc cgagtatggt caagtatctg
    13141 gttagaggtt tcctgcatga tgtggatggg gttatttgcc ctagtgagat tgtccgtgac
    13201 ttgctatctg attataaggt caaggttgaa aaacgggtca ttcctactgg gattgaatta
    13261 gccaagtttg agcgtccgga aatcaagcag gaaaatttga aagaactgcg tagtaaacta
    13321 gggattcaag atggtgaaaa gacgttgctt agtctttcga gaatctccta tgaaaaaaat
    13381 attcaagcag ttttagcagc ctttgctgat gttctgaaag aggaagacaa ggttaaactg
    13441 gtagtagctg gggatggccc ttatctgaat gacctcaaag agcaagccca gaacctagag
    13501 attcaagact cagtcatctt tacagggatg attgctccta gtgagacggc tctttactat
    13561 aaagcggcgg atttcttcat ttcggcatcg acaagcgaaa cgcaaggttt gacctacttg
    13621 gaaagcttag ccagtggaac acctgtcatt gctcacggaa atccttattt gaacaacctc
    13681 atcagtgata aaatgtttgg agccttgtac tatggagaac atgatttggc tggtgctatt
    13741 ttggaagccc tgattgcaac accagacatg aatgagcata ccttatcaga gaaattgtat
    13801 gagatttcag ctgagaactt tgggaaacga gtgcatgagt tttatctgga tgccattatt
    13861 tcaaataact tccagaaaga tttggctaaa gatgatacgg tcagtcagcg tatctttagg
    13921 acagttttgt atcttccgca acaggtggtt gctgtacctg taaaaggatc tagacgcatg
    13981 ttgaaggctt caaaaacaca gttgatcagt atgagagact attggaaaga ccatgaagaa
    14041 tagaaagagg aacagctatg aaaaaaacaa ttaatgagaa gcggtcgtga taaaaagatt
    14101 gcgggtgttt gtgctggggt ggcccattat ctggatatgg atccgactat cgttcaagtc
    14161 atttggggtg ttcttacttg ctgttacgga gctggaattg tagcttacat tattttatgg
    14221 attatcgcac cagtagcaac tgactattga aaactagctc aattcatgct aagataatag
    14281 aaaatagaat gtaacgaagg agagaaaaat ggcatttgga gataatggaa atcgtaaaaa
    14341 aactatgttt gagaaaataa ccttgtttat cgtgattatc atgctagtag caagtttatt
    14401 gggaattttt gcaactgcaa ttggtgccct cagtaatcta taaaatagat tcaagaaaat
    14461 ttagtgactg ggatttccca gccctttttt aaagtgagaa gaaataatga gtatgttttt
    14521 agatacagct aagattaagg tcaaggctgg taatggtggc gatggtatgg ttgcctttcg
    14581 tcgtgaaaaa tatgtcccta atggaggccc ttggggtggt gatggtggtc gtggaggcaa
    14641 tgtggtcttc gttgtagacg aaggactacg taccttgatg gatttccgct acaatcgtca
    14701 tttcaaggct gattctggtg aaaaagggat gaccaaaggg atgcatggtc gtggtgctga
    14761 ggaccttaga gttcgagtac cacaaggtac gactgttcgt gatgcggaga ctggcaaggt
    14821 tttaacagat ttgattgaac atgggcaaga atttatcgtt gcccacggtg gtcgtggtgg
    14881 acgtggaaat attcgtttcg cgacaccaaa aaatcctgca ccggaaatct ctgaaaatgg
    14941 agaaccaggt caggaacgtg agttacaatt ggaactaaaa atcttggcag atgtcggttt
    15001 agtaggattc ccatctgtag ggaagtcaac acttttaagt gttattacct cagctaagcc
    15061 taaaattggt gcctaccact ttaccactat tgtaccaaat ttaggtatgg ttcgcaccca
    15121 atcaggtgaa tcctttgcag tagccgactt gccaggtttg attgaagggg ctagtcaagg
    15181 tgttggtttg ggaactcagt ttctccgtca catcgagcgt acacgtgtta tccttcacat
    15241 cattgatatg tcagctagcg aaggccgtga tccatatgag gattacctag ctatcaataa
    15301 agagctggag tcttacaatc ttcgcctcat ggagcgtcca cagattattg tagctaataa
    15361 gatggacatg cctgagagtc aggaaaatct tgaagaattt aagaaaaaat tgtctgaaaa
    15421 ttatgatgaa tttgaagagt taccagctat cttcccaatt tctggattga ccaagcaagg
    15481 tctggcaaca cttttagatg ctacagctga attgttagac aagacaccag aatttttgct
    15541 ctacgacgag tccgatatgg aagaagaagc ttactatgga tttgacgaag aagaaaaagc
    15601 ctttgaaatt agtcgtgatg acgatgcgac atgggtactt tctggtgaaa aactcatgaa
    15661 actctttaat atgaccaact ttgatcgtga tgaatctgtc atgaaatttg cccgtcagct
    15721 tcgtggtatg ggggttgatg aagcccttcg tgcgcgtgga gctaaagatg gggatttggt
    15781 ccgcattggt aaatttgagt ttgaatttgt agactaggag actggtatgg gagataaacc
    15841 gatatctttc cgagatgcgg atggtaattt tgtttccgcc gcagacgttt ggaatgaaaa
    15901 gaaattggaa gaactattta atcgtctcaa tccaaatcgt gccttgagat tggcacgaac
    15961 taaaaaggaa aatccatctc agtaaagaag ctaaaaaatc ccgtgcctca tcagacacgg
    16021 gattttgtgg tacgacaggc atgtatagca aactgaatct ggaatagcac agcatatctt
    16081 ctaaaatatt gttataaatt gatttgaatt tcaaaattaa attgtttgat tcttatctca
    16141 atttgttata gtatatctga tgtcaaagtt ctcggcgagt caaatagcga ttcccaagcc
    16201 tgactatcgt gaggtagcgg attaaaatgg tctggggata gaccgtttta agtctgacgc
    16261 tggaaataag aattgtcaga agaagggata gcgaaatcgt ggctctacga acaggaacgt
    16321 gataataagg cgtatatagc ggataagagg gcctcaaact ctaaagtcca aaaaggtagt
    16381 cgtaacctat atgcgtaaat cacgagagta attgaattcg tactaagatt ttctattttc
    16441 actgtaacct tttaacgtcc ttatatcttg tatacacgag gaaagatgta cgacttatcc
    16501 cgtgaggtct atcactataa agagaaaacg acagatagaa gtgatcctga gtcacggtta
    16561 tctttaaaat agattgtctg ataggacggt atgtataaaa cgcttctgtg aactgagaga
    16621 agggggagaa gttcttgcta aaatttagtt gaacagccgt attccgatac ttagataaga
    16681 gatctagtct tagctcctac tcagttttag gggataaaaa aggggaaata gcgattcgag
    16741 aaagattata ctcttcgaaa atctcttcaa atcacgtcaa tatcgccttg ccgtatgtgt
    16801 aggatactga ctacgtcagt tccatctaca acctcaaaac agtgttttga gcaacctgcg
    16861 gctagtttcc tagtttgatc tttgattttc attgagtatt agtaattcag ttactaactc
    16921 gtcaactctg atttatccaa taaaattgaa aaggatggaa aaaaggataa atttatgata
    16981 tactttattt tgaagacctt attagaaatc ttgaaagagt attgaaaact tagaatgaga
    17041 aaaattgtta tcaatggtgg attaccactg caaggtgaaa tcactattag tggtgctaaa
    17101 aatagtgtcg ttgccttaat tccagctatt atcttggctg atgatgtggt gactttggat
    17161 tgcgttccag atatttcgga tgtagccagt cttgtcgaaa tcatggaatt gatgggagct
    17221 actgttaagc gttatgacga tgtattggag attgacccaa gaggtgttca aaatattcca
    17281 atgccttatg gtaaaattaa cagtcttcgt gcatcttact atttttatgg gagcctctta
    17341 ggccgttttg gtgaagcgac agttggtcta ccgggaggat gtgatcttgg tcctcgtccg
    17401 attgacttac accttaaggc gtttgaagct atgggtgcca ctgctagcta cgagggagat
    17461 aacatgaagt tatctgctaa agatacagga cttcatggtg caagtattta catggatacg
    17521 gttagtgtgg gagcaacgat taatacgatg attgctgcgg ttaaagcaaa tggtcgtact
    17581 attattgaaa atgcagcccg tgaacctgag attattgatg tagctactct cttgaataat
    17641 atgggtgccc atatccgtgg ggcaggaact aatatcatca ttattgatgg tgttgaaaga
    17701 ttacatggga cacgtcatca ggtgattcca gaccgtattg aagctggaac atatatatct
    17761 ttagctgctg cagttggtaa aggaattcgt ataaataatg ttctttacga acacctggaa
    17821 gggtttattg ctaagttgga agaaatggga gtgagaatga ctgtatctga ggacagcatt
    17881 tttgtcgagg aacagtctaa tttgaaagca atcaatatta agacagctcc ttacccaggc
    17941 tttgcaactg atttgcaaca accgcttacc cctcttttac taagagcgaa tggtcgtggt
    18001 acaattgtcg atacgattta cgaaaaacgt gtaaatcatg tttttgaact agcaaagatg
    18061 gatgcggata tttcgacaac aaatggtcat attttgtaca cgggtggacg tgatttacgt
    18121 ggggccagtg ttaaagcgac cgacttaaga gctggggctg cactagtcat tgctgggctt
    18181 atggctgaag gtaaaactga aattaccaat atcgagttta tcttacgtgg ttattctgat
    18241 attatcgaaa aattacgtaa tttaggagcg gatattagac ttgttgagga ttaaaccgta
    18301 gaggtgttta tgaatatttg gaccaaatta gcaatgtttt ctttttttga aacggatcgc
    18361 ttgtatttgc gtcctttctt ttttagtgat agtcaggact tccgcgagat agcttcaaat
    18421 ccagaaaatc ttcaatttat tttcccaacg caggcaagtc tggaagaaag tcaatatgca
    18481 ctggccaatt actttatgaa gtcccctttg ggagtgtggg caatttgtga ccagaaaaat
    18541 caacaaatga ttggttctat taaatttgag aagttagatg aaatcaaaaa agaagctgag
    18601 cttggctatt ttttgagaaa agatgcttgg tcgcaaggat ttatgacaga ggttgttaga
    18661 aaaatttgtc agctttcttt tgaggaattt ggcttaaaac aattatttat cattacccac
    18721 cttgaaaatg aagctagcca aagagttgct cttaagtctg gatttagttt gttccgtcag
    18781 tttaagggaa gtgatcgtta cacaagaaaa atgcgggatt atcttgaatt tcggtatgta
    18841 aaaggagagt tcaatgagta agcatcagga aattctaagc tatttggagg aattaccagt
    18901 aggtaaaagg gtcagtgttc gtagcatttc gaatcatcta ggagttagtg atggaacagc
    18961 ctatcgagct attaaagaag ctgaaaaccg tggaattgtg gagacccgtc ctagaagtgg
    19021 aacaattcgt gttaaatccc agaaagttgc tatagataga ttaacgtttg ctgaaattgc
    19081 agaagtgact tcttctgagg ttctggctgg gcaagaaggt ttagagagag aatttagtaa
    19141 gttttcaatt ggtgccatga ctgaacaaaa tatcttgtct taccttcatg atggggggct
    19201 cttgattgtc ggagaccgaa cccgtattca gttgctagcc ttggaaaatg aaaatgcagt
    19261 tctggttaca gggggatttc aggttcatga tgatgtgctt aaactggcca atcaaaaagg
    19321 gattcctgtt ctaagaagta agcatgatac ctttaccgtc gctaccatga tcaataaagc
    19381 cttgtcaaat gtccaaatca agactgatat tctgacagtt gagaaacttt atcgccctag
    19441 tcatgagtat ggttttctga gagagacaga tacagttaaa gattatttgg acttggttcg
    19501 taagaatcgt agcagccgtt tccctgttat caatcaacat caggtcgttg ttggcgttgt
    19561 aaccatgaga gacgctggtg ataaatcacc aagcacgaca attgataagg ttatgtctcg
    19621 tagtctattt ttggttggat tatcgacaaa tattgccaat gtgagtcaac ggatgatcgc
    19681 agaagacttt gaaatggtac cagttgttcg aagcaatcaa actttgcttg gcgttgtgac
    19741 gcgacgagat gtcatggaga agatgagccg ttcccaagtt tcggctctac caactttttc
    19801 tgagcagatt ggacaaaagc tctcttatca ccatgatgaa gtagtcatta cagtggaacc
    19861 ctttatgcta gaaaaaaatg gagttttggc taatggtgta ttggcagaaa ttctgaccca
    19921 catgacccaa gatttagttg ttaatagtgg tcgcaatctc attatcgagc agatgctgat
    19981 ctactttttg caggccgttc agatagatga tatattgcgc attcaggcac ggattattca
    20041 tcatacgaga cggtcagcta taattgatta cgatatttat catggtcacc agattgtttc
    20101 aaaagcaaat gtgactgtta aaattaatta gaaactagga gaaaagatga taacattaaa
    20161 atcagctcgt gaaatcgaag ctatggacaa ggctggtgat tttctagcaa gtattcatat
    20221 aggcttacgt gatttgatta agccaggcgt agatatgtgg gaagttgaag aatatgtccg
    20281 ccgtcgttgt aaagaagaaa atttccttcc acttcagatt ggggttgacg gtgccatgat
    20341 ggactatcct tatgctacct gttgctctct taacgatgaa gtggctcacg ctttccctcg
    20401 tcattatatc ttgaaagatg gtgatttgct caaagttgat atggttttgg gaggtcccat
    20461 tgctaaatct gacctaaatg tctcaaaatt aaacttcaac aatgttgaac aaatgaaaaa
    20521 atacactcag agttattctg gtggtttagc agactcatgt tgggcttatg ctgttggtac
    20581 accgtccgaa gaagtcaaaa acttgatgga tgtaaccaaa gaagctatgt acaagggtat
    20641 tgagcaagct gttgttggaa atcgtatcgg tgatatcggt gcggctattc aagaatacgc
    20701 tgaaagtcgt ggttacggtg tagtgcgtga tttggttggt catggtgttg gcccaactat
    20761 gcacgaagaa ccaatggttc ctaactatgg tattgcaggt cgtggactcc gtcttcgtga
    20821 aggaatggtc ttaaccattg aaccaatgat caatacaggc gattgggaaa ttgatacaga
    20881 tatgaaaact ggttgggcgc ataagaccat tgacggtgga ttgtcatgtc agtatgaaca
    20941 ccaatttgtc attacgaaag atggacctgt tatcttgact agccaaggtg aagaaggaac
    21001 ttattaataa aaagtgaaaa gactactgga agtttattat gaaaaaatcc agtagatctt
    21061 ttcataataa aacgcattgt atcaagtgtt aggggctgat atcatgcgtt tttctgcttt
    21121 taagattttt tccaactctg tttgtaagcg catcataaca aagggtctag gattcagggc
    21181 tctcctccta tatactatta gtaaagtaaa actaagggag gatattttag tgtcgcagtc
    21241 tattgttcct gtagagattc cacaatattg tcgttttgat tctaaaaaga gaaatggaat
    21301 tctgtttaat gttcgtattg ccaatcttaa atttaccttt tttagattag acttcctgcg
    21361 aaacaaaaca tggtatagta gttctatgaa tgatgaagca agtaaacaac taactgatgc
    21421 acgatttaag cgtcttgttg gtgttcaacg cacgactttt
//
LOCUS       DAVBBS010000036        21070 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVBBS010000036 DAVBBS010000000
VERSION     DAVBBS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21070
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.036"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            182..658
                     /locus_tag="VQU32_001322"
     CDS             182..658
                     /locus_tag="VQU32_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2472771.1"
                     /translation="MKLSLYSEVEHYHFLSSYSLNDITYTDTPKNIIEKSKKDKYRYP
                     IVILNKDHKVIGFFCLHLNTGPKEYGYYQNDYALIRGFSIHDNFRNQGYGSNALNEIF
                     EFIDSTLKLEINHIILAVNEKNILAQKAYRNSGFSVVKKDLEGKRGKLIIMEKSRN"
     gene            complement(1332..1724)
                     /locus_tag="VQU32_001323"
     CDS             complement(1332..1724)
                     /locus_tag="VQU32_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET2472772.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLTRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTDKSQYEFLFPSVK
                     IKEEKTDKKEEVATDSSEKAEKKIRREA"
     gene            complement(1702..2163)
                     /locus_tag="VQU32_001324"
     CDS             complement(1702..2163)
                     /locus_tag="VQU32_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET2472773.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVGQLEAELDRSQFEKVEGNRLYMKQDDKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            complement(2126..2386)
                     /locus_tag="VQU32_001325"
     CDS             complement(2126..2386)
                     /locus_tag="VQU32_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472774.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            complement(2391..2819)
                     /locus_tag="VQU32_001326"
     CDS             complement(2391..2819)
                     /locus_tag="VQU32_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET2472775.1"
                     /translation="MEGQIVKSMIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVE
                     EQIFFMEFEELYRETQKRSVASQQKTSLNLDGQMISNGSQKLTVPKGIQAPSGQSITF
                     DRAGGNSSLAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            complement(2788..3114)
                     /locus_tag="VQU32_001327"
     CDS             complement(2788..3114)
                     /locus_tag="VQU32_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET2472776.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLDKNEDATLSKLQGDGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            complement(3116..4132)
                     /locus_tag="VQU32_001328"
     CDS             complement(3116..4132)
                     /locus_tag="VQU32_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET2472777.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLDFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            complement(4080..5021)
                     /locus_tag="VQU32_001329"
     CDS             complement(4080..5021)
                     /locus_tag="VQU32_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HET2472778.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            complement(5097..5462)
                     /locus_tag="VQU32_001330"
     CDS             complement(5097..5462)
                     /locus_tag="VQU32_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HET2472779.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            complement(5613..6671)
                     /locus_tag="VQU32_001331"
     CDS             complement(5613..6671)
                     /locus_tag="VQU32_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET2472780.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPKKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            complement(6834..7985)
                     /gene="nagA"
                     /locus_tag="VQU32_001332"
     CDS             complement(6834..7985)
                     /gene="nagA"
                     /locus_tag="VQU32_001332"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HET2472781.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            complement(8138..9955)
                     /locus_tag="VQU32_001333"
     CDS             complement(8138..9955)
                     /locus_tag="VQU32_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HET2472782.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMIFLLSATAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            complement(10056..11198)
                     /gene="tgt"
                     /locus_tag="VQU32_001334"
     CDS             complement(10056..11198)
                     /gene="tgt"
                     /locus_tag="VQU32_001334"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET2472783.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMLLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAECGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFRKYFVEKYGYNKSGRNF"
     gene            11328..12185
                     /locus_tag="VQU32_001335"
     CDS             11328..12185
                     /locus_tag="VQU32_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HET2472784.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            complement(12213..12857)
                     /gene="pcp"
                     /locus_tag="VQU32_001336"
     CDS             complement(12213..12857)
                     /gene="pcp"
                     /locus_tag="VQU32_001336"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET2472785.1"
                     /translation="MKVLVTGFEPFGGEKGNPTLEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSVTPERVAINQDDARISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKFPYVK
                     AGFMHIPYMMEQVVNRPTTPTMSLVDIRRGIEAAIGAMIEHGDQELKLVGGETH"
     gene            complement(12964..13332)
                     /locus_tag="VQU32_001337"
     CDS             complement(12964..13332)
                     /locus_tag="VQU32_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HET2472786.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATRSDATSRVFNMEKEELA
                     HPSVSSLFKNQGIYKALLGVFLWYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKYT"
     gene            complement(13343..13768)
                     /locus_tag="VQU32_001338"
     CDS             complement(13343..13768)
                     /locus_tag="VQU32_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET2472787.1"
                     /translation="MIEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIKVKEEMESILTEFERTELSRLLNKLVLGIENIEI"
     gene            complement(14193..15335)
                     /locus_tag="VQU32_001339"
     CDS             complement(14193..15335)
                     /locus_tag="VQU32_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET2472788.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETSSAVAEEAPQGTTPAEKQETQASPQAASAVEATTTS
                     SEAKEVASSNGATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            complement(15501..16121)
                     /locus_tag="VQU32_001340"
     CDS             complement(15501..16121)
                     /locus_tag="VQU32_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2472789.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQNLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNTFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            complement(16125..17405)
                     /locus_tag="VQU32_001341"
     CDS             complement(16125..17405)
                     /locus_tag="VQU32_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET2472790.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKLTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            complement(17466..18950)
                     /locus_tag="VQU32_001342"
     CDS             complement(17466..18950)
                     /locus_tag="VQU32_001342"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET2472791.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKGGVSKVQELQMTTQNGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTAELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVYESDS
                     YIEDPHTAVASAVYRKYQAVTGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            complement(19026..20303)
                     /locus_tag="VQU32_001343"
     CDS             complement(19026..20303)
                     /locus_tag="VQU32_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HET2472792.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            complement(20410..20493)
                     /locus_tag="VQU32_001344"
     tRNA            complement(20410..20493)
                     /locus_tag="VQU32_001344"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20457..20459),aa:Leu,seq:caa)
     gene            complement(20503..20574)
                     /locus_tag="VQU32_001345"
     tRNA            complement(20503..20574)
                     /locus_tag="VQU32_001345"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20540..20542),aa:Gln,seq:ttg)
     gene            complement(20588..20660)
                     /locus_tag="VQU32_001346"
     tRNA            complement(20588..20660)
                     /locus_tag="VQU32_001346"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20625..20627),aa:His,seq:gtg)
     gene            complement(20675..20745)
                     /locus_tag="VQU32_001347"
     tRNA            complement(20675..20745)
                     /locus_tag="VQU32_001347"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20711..20713),aa:Trp,seq:cca)
     gene            complement(20750..20830)
                     /locus_tag="VQU32_001348"
     tRNA            complement(20750..20830)
                     /locus_tag="VQU32_001348"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20794..20796),aa:Tyr,seq:gta)
     gene            complement(20843..20915)
                     /locus_tag="VQU32_001349"
     tRNA            complement(20843..20915)
                     /locus_tag="VQU32_001349"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20880..20882),aa:Phe,seq:gaa)
     gene            complement(20919..20992)
                     /locus_tag="VQU32_001350"
     tRNA            complement(20919..20992)
                     /locus_tag="VQU32_001350"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20956..20958),aa:Met,seq:cat)
BASE COUNT         6295 a         4801 c         3726 g         6248 t
ORIGIN      
        1 tggtattatc aatcatgaac taggattcta gttttgcagg aagtctaata atgaaaataa
       61 aggactaaag caataactct aaatacatct gatttttata ggatatttca taaaaaaaat
      121 ctacatacct agagtcctat ttagttatgt atcttacata aagagaaagg aattaattat
      181 catgaaatta tctttgtata gtgaagttga acattaccat tttttaagtt cttattcttt
      241 aaatgatatt acatatactg ataccccaaa aaatattatt gaaaagtcta aaaaagataa
      301 gtatagatac ccaatagtaa tacttaataa agaccataaa gtaattggat ttttttgcct
      361 acacctaaac actggtccta aagagtatgg ctattatcaa aatgattatg cgcttattag
      421 aggattttct attcatgata atttcagaaa tcaaggatat ggttctaatg ctctgaatga
      481 aatatttgaa ttcatagact caactttaaa attagaaatt aaccatatta tattggctgt
      541 aaatgaaaaa aatatattgg ctcaaaaagc atatagaaat tccggttttt ctgttgtgaa
      601 aaaagattta gaaggaaaaa gggggaaatt aattattatg gagaaaagca gaaactagta
      661 atgatactag tttctgcttt tctctaagtc tgctataaat ttataacatg ttgaaataag
      721 atgtgaataa attgattgca gaattcaaac caatttctaa caatgtttta gaaaactcgg
      781 cggactattc cagattcaat ccactatatt ctatcttatt tgcatatttt gagagaaata
      841 atctatatac taaactttta tgtttataat tcattccttt cgtttcactc aaggcacaag
      901 acagaatgaa aaagtggtgt gctctttatt ttgttttata ataatagtga gaaaacctat
      961 cactactaca aatcacgggg aggtgaataa gtgagtggta tagccactac ctcgcatttt
     1021 tataggtgtc attctttcca tcaagcacaa gaactgtgac taggagcacg taaacttata
     1081 attgaataag cgactcgttt gtgaaaggaa agaatagtca agggataggc agactcaaat
     1141 ttggtgaagg gaaacccctc atgttaaaag ccaaacttca attttaaaaa ttttaaaatt
     1201 tgtttcgtat tcactactcc ttgcctttat attggattga ttaagagaac ctttcgcgtt
     1261 tctagaaact acagtggact attctggatt caacgcatta tagttgaatt gactaggaat
     1321 tctctttctt ttcaggcttc tcttctgatt tttttctccg ctttttcgct tgaatcagtc
     1381 gctacctctt cctttttatc cgttttctct tctttgattt tgactgaagg aaacagaaac
     1441 tcatattgac tcttatccgt acgaacagtc gtcaccaagc ttgttttctt gttttgatag
     1501 ctcacctgac ctagattaaa aacctgttcc ccactttctt gctcggcttt atccttggtt
     1561 cgtttagcca tggcaaaagc aaccaatttt tctttattta aagcatagtc ttgatagtgg
     1621 gcgatttgtc gggtcaaata aaattgcaac aaaagactaa agatggctgc tatggtgact
     1681 gcgtagagga gaacacctgc cttaactttc ttctttttcc acacgataga tgaactccct
     1741 ttctaagcct ttttgaaact ggaaacgaaa atgaaccagt tgattgtcct ctgtaatctg
     1801 agctgatttg aggccataaa ccataggctg atacccccgt ccactagcat cggttttacg
     1861 aaaatcgtct gactttgact taccgatggc gatgtccttg tcatcttgct tcatgtatag
     1921 gcgattgcct tctacttttt cgaactgcga acggtctaat tctgcctcaa gttggcccac
     1981 aaacaagagc cactcctttt gctcgctttg ttgctggtag cgaacttctg aaatgaggag
     2041 ctgactcata gcttgaaaaa ggagtaatcc cccactgatg acaatgaggg caagcaggga
     2101 ttctaacaag gtaaaagcct tgaccttatg gctctttgat tgccaacaac tgttctgaac
     2161 catggtagac ctccaatccc ttttcactag aaaatacctg aatctcaact ccgttgatgc
     2221 ttacctgatt ttgccccgtc tgcaaggcca tcttagctac cctcaagact tcttcctttt
     2281 gcaagatttt tgcttcctct tgcctatttt tttgaatttg tcccaaaagg agggtcgcaa
     2341 tgctggcaaa gatagctaga gcgactactg cttccagtaa aatcactgcc ctaatttttt
     2401 gtttccttaa tgcgtttaat ttttccattt cctagatata attgatagcg aatcgctcct
     2461 ttactggtct gaaattcaac cttagccagg gacgaattgc ccccagctcg gtcaaatgta
     2521 atactttggc ctgatggtgc ctgaattcct ttaggaactg tcaacttttg actgccattg
     2581 ctaatcatct gcccatccaa gttcagacta gtcttttgtt gactagctac actgcgtttt
     2641 tgggtttccc gatagagttc ttcaaactcc ataaagaaaa tctgttcctc taccgctgca
     2701 aaagtggact ggacagagcc ggacaagccc aaggcaagga tactcacaag tcccaaaacc
     2761 aagagacttt ccagcatggt aaaggcctta atcattgact ttacgatttg cccctccatt
     2821 tttagtatag tattcattat aagctttaac ctgttcttcc gtgatgcgcc catctccttg
     2881 taacttgctc agagtagcat cttcattttt atccaagcta taaagttctg cctggctttc
     2941 caccacctta acaacagctg cttttccttt gtcattgact gcttcttttt gcttggtcag
     3001 attaggtaca aagagcaaga aaagcacgct gataatcagc aagaccacca acatctccac
     3061 caatgtaaaa gctttaacct tagctttttt caagaatgtc atcatttttt tcattttaaa
     3121 aatttacctc catattttga tacatgggca tgagcattgc cgcataaagt aaaacgataa
     3181 tcagtgccac aaagataaaa accagtggct gcaccaaatt catggtgcgg ttgactcggg
     3241 taaaaaaggc ttcccaagtt ttttcagcat agatttccaa ctcactaccc agcttggact
     3301 taacttcccc atactctatg atgagactca attccttcct aaagaaagga taggttccta
     3361 tcgtctgaga aaattcacgg ccatttttca gggtttgagc cagatcttga ccgatttctt
     3421 taaagagctg ggaaccttgt tcctgcatca tttgaaaaat ctgcgtcagc tccattccct
     3481 gtgaaatcat attcccccat tcacgtgcat aataggctgt caagtaggtc tgcacaaaga
     3541 ttccaataaa gggaaggcgt gctaagatag aaaagacact catcttagaa cttcttttat
     3601 aaaaagttag tgctaaaagg gcaagcacgg aaacaagccc taccatgcct agaaaaattt
     3661 gtggcagatt accgataatt cgggtggcaa tattgctact atccagttgt gggagcaggt
     3721 aattccgtag ccccagcata attaagagaa gaaaatccag caaaatcaaa ggataggtcg
     3781 ctacttcaat taactttttc ttgaccttgg ccagattgtc tagatattct tctatctttc
     3841 ccaaactcag gtgaagattg ccatgaactt cagctagaga taactgagtg acaatggtac
     3901 ttgaacatcc caaactttcc atcatttctg agaatgattt cccctgagac aagcctgtac
     3961 gcatctgggt cacacactgc ttgtccaaca aggaactcct atctaaaaag gagatagtct
     4021 ccaccagatg aaaaccgcta gaaaagagat tgttaaatag ggtgatgata tttttttgct
     4081 tagctgtagc taattttttc cgtctcagcc tgaagacttg tgatatgtcc atctttaaga
     4141 agttggtcaa tttgctcatt ccacttggct gcttggtgtt cttgataatc tctgtttgca
     4201 aagtcaacga ttcctcctcc cccgattaat ctctggtagc agactccttg cagaacaact
     4261 gccaattctt cttcactcac acccaactcc agcagacgct cataaacacc tcggatactc
     4321 ttggcgtgaa tggttgaaaa gactgtcgca cctgtcaaac tagctctgac cactgcacgc
     4381 gccgtctcgc tgtcacgaat ttctccgata atcaagagat ctggtcgatg acgcaaggaa
     4441 agtttgatta gattttcata ggttaggccg attgcttcgt tcaactgcaa ctgaagcatg
     4501 tcgtcctgct tgatttcgac aggatcttcg atggacataa cttgctgtcc tttaaagagt
     4561 gacttggaca attcatgcat caaggtcgtc ttaccactcc caaccgggcc agcaaaaaga
     4621 tagagtcccc gttgcctgta ctgcttgcct aattcttcaa tatcctgaaa ccaaaaatgc
     4681 aagtcctgct cctcatcgtg caacaaacgg ataaccaaac tctcatgccc ccgataatcg
     4741 cctacagtag ataaacgtag agacgccatc ttctggtcat aggcataatc acaggaaccc
     4801 agttgactac gtcttttttc tcccacattc atacccgcca caaacttaaa gtgactgata
     4861 acggctgcaa acttttcaaa atcatagcta ccaattttac agcgctcgtc tcctaccctc
     4921 atatgaagct cataggcgtc taacttaggg acaaaataaa tatcctgcgt cccttttttc
     4981 cgagctgaac gaatgatttc ttgtgcaatt tcttgaacca tacttacctc ctcacctata
     5041 ctattcgcaa agcgttagaa aaataaaaaa agcaactcca aaaaagttac ttttctctat
     5101 tttaatttca tacggcaaga acgattcctt tcctgactgg tttgtttttc atagcctgag
     5161 cgtagttttt cgtctgggat aacctgctca tcctgcaaaa gagccaaaga atggtattgt
     5221 tgtaaatact catcacattc atcacaccag cgttggtctt ccgggtccca aaaaatggtc
     5281 atcaagtcgc ttctactttt ataaagaggg gattcttgtt ccaatctaaa ccagcaagtt
     5341 ttgtagtatt tgagagcatc ataatactgg tcaaattttc tacttgctac aatatcttct
     5401 tcccaacctt ctaagaacca ccacggttca aaatctccgt acatttctat aacacgatac
     5461 atattcattc attcctttgt accgtatatt ataaccaatt ccgccagaca aggaaagaaa
     5521 tatgctcatt tcttaatttt ttgataaaaa atccagccat ctgatggatg actgaatttc
     5581 tatttgctta tgtacaaaaa tatttttact acttaggctt ctgagatatc gttttcgata
     5641 atgaccttaa tggcatggtg gtctgctgcc ttactgaaga cttcgtaggc tttttcaatt
     5701 tcactgagtt tgaaatagtg agttaccaat ttttccggtt caatcttatg actttcaagt
     5761 gctttcaaca attgtggagt cgtatttgta gataccaaac cagttgttac attgatgttg
     5821 cgaatccaaa gtttatctaa atcgaattca actggtttac catgcacacc acagttggca
     5881 accgttccgt ctacaccgat aatcttttga cagaaatcaa atgtttcagg aatccctaca
     5941 gcttcgatag cgacatccac accacgacca tctgtcaaat cataaatttc tttaatggct
     6001 tttttagggt ctgaagaatt aaccttatga gtcgcaccga atgatagggc agtttccaag
     6061 cggttatcgt ctaggtctac cataatcaat ttagctggtg aatagaattg ggctgttaaa
     6121 agagcagcca atccaactgg acctgaacca ataatggcta cgctgcaacc aggttctact
     6181 ttccctttta agacaccaat ttcatatcca gtaggcagaa tgtctgacag cataaccaaa
     6241 gcttcatctg acaagtcttc tggagtatgg taaagagtat tatctgcatg agggacacgt
     6301 agatattcag cctgcatacc atcaatcaag tgaccgaaaa tccagccccc ttcgtcttca
     6361 cagtgggcat aaattccttt tttacagtag tagcatttac cacaggcaca gacgcaagaa
     6421 atcaagacct tgtcaccttt tttgaagttg gaaactcctt ccccaacttc ttcaacaatc
     6481 ccaatccctt cgtggccaag aatggtacca ctttggcaag taggaacatc ccctttgata
     6541 atatggaggt ctgttccaca aatagtggtt tttacaatac gcacaatagc gtctgttggc
     6601 ttgcgaataa ctggtttgtc tacatcaaca aaagaagcaa gtcctggttt aacataagta
     6661 taggctttca taaagcactc ctccgttttt ttatttgtaa tatttatagt tcaattgtaa
     6721 gcctttttct ttgtttcttc aagctttttt tgtgattttt ttaacttttt atttaccagt
     6781 aaagtaccct gtataaaaaa gcagaagcta gtctctaact tctgccttct ctcttatgct
     6841 tgataacgtt tcacaccatc taggtaggtt gctaccaatt ccaaatcttt atctaatacg
     6901 atgaagtcag cgtcgtagcc ttcacggatt tggccacaga cgtcatcgat gtgaacggat
     6961 ttagctgggt tgaagctggc catcatgact gcttcatgcg gattcgcaat tccccattcg
     7021 accacattct tcaaaccatc tttgagtttg aggatagaac ctgccaagtt acctgtcgat
     7081 ttgaggcgtg cagttccatt tgcaacgaca actgggaatt ctcccaacat ataatctccg
     7141 tcttccaatc ccccagctgt catacagtct gtgataagag cgatgttttc tgttcctttt
     7201 tgtttgataa gaatttcgca agcctttgga tctacgtggt gaccatcaca gatcaactct
     7261 gcataggtat gtggcaattg gtacatggct ccaaccatac ccaattcacg gtgagtcaac
     7321 ccacgcattc cattgtaggc atgcacccaa acactcgctc cagcatcgac tgcttttttg
     7381 gcttcatcaa aagtcgcgtt tgaatgtcca agagcaaccg tcacaccttc gcccgtaact
     7441 gtacgaacaa agtcttccac cccatcacgt tctggtgcaa gggcaatttt attaagcaag
     7501 ccatttgccg ctttttgcca agaatgaaac tcctcaacac ccgggtctct catataagtt
     7561 ggattttgtg cccccttaaa agtttctgtg aaatatggac cttcataata aatcccacga
     7621 atcttagcac ctgttgcttc tttataatgg tttccaagat tttcagtgac tgcaagcaat
     7681 tgctcataag tggctgttaa agttgtgggt aagaaactgg taacaccggt actaagaagt
     7741 ccttcactca tagtatgcaa tgtaccttca atgttgttgt ccatcacatc tacacctgca
     7801 tatccatgaa tatgagtatc cacaagtcct ggggcaatgc tataacctgt atagtcaatc
     7861 acctcagccc cttcaggaat ctgctctaca tgtttcccaa acttgccgtc cacaagttcc
     7921 aagtaaccac ctcgacgaac tccgtgtggg tagaaaaact gatccgcttt aatatagtta
     7981 ggcataatgt taacctcctt aaaagattga ttctacaatt tattatgtca attacattat
     8041 aaacctttct tttttatttg taaatggtat agacctattt tttggagata ttttaaaaga
     8101 gctggatttc tccaactctt ctctttttta atacaagtta ttttgattta accggcttgt
     8161 cttgagctgt ctgcaaagct gtggcaatcg tatctgcata caattttgct cctgcttcga
     8221 tagtgctact ctcactcccg aaatgaacct ggtctgttcc agcccaaatt tctggatgct
     8281 ctttcgcaac ttgattccaa tctgctatcg taatgtaagg tgtcttctct gccaattctc
     8341 tcatataggc agcagccttc tcaacgatgg cataggtctc ttttgtctta tctccctcat
     8401 aaggagtcac caaaatcata tggtgtccct taggaagatt tttcacgata ctgtcccagt
     8461 catccttgta attctcagga ttatttaccc cagtcgcaat gaccaccgtc ttaggtaaaa
     8521 atttattctg gctattattt agcatgattt catttgcggt cttggttgtt acgctgacct
     8581 gcgcgttaat ctgtgctcca ggaagagctg tctgtagtgc tgtatttgcc cttaaagcca
     8641 ctgagtcacc aattaacata gtgccatcag caattcccaa actgtttgca tctgcccgtt
     8701 ctgccatcac cttggtctgg ccaatatttg ttgcagcttg cttcaagcca ttgacagtca
     8761 agtctgtctc aaacgctccc acttgtggtg ccaacaaggt caccatgcag acaatgaagg
     8821 tcaagattcc tgtacctgct gcaagaattg cgtgaatata aggcagggga cgaagagttt
     8881 gtaaaatagg tgtgttcttg cctgcaatcc aaggttccaa tacataaaat gacagactgg
     8941 caaagccata agaacaaatc agagtcagta atacagcaag aagatttgat gtcaactgtg
     9001 agaaaatgat atagaaaggc caatggaaaa gataaaccgc atagctagta tccgctaaaa
     9061 agctgataat cttcggctcc tgtatatgat gtgtcttttc atgtaagaca cgcgccgcca
     9121 gaatcatggc aagagctgca agactggcaa gcaagaagcc gataagatag gcaaaaagat
     9181 aagtgaattt gacaaagaaa gtcaaaagaa ctaggaaacc aaagcctcct ccaaaaacta
     9241 ctaaagtctt tcgtaaatcc cagattttat ccaactgctt gacgagggaa gtcgtctgac
     9301 gaacgcctac aatagttgct aacatacttc ccaaaaagaa tggatagaca tgagttaaac
     9361 tggagaaata aacagaggaa taagaggtca ctagaaaact accaataaac atggagaaga
     9421 aactgatcaa gaaggcagta gcagataaga gaaagatcat ccccttcaac tgaccatttg
     9481 atttagcttg tttggataag aaccaaactg ccaatcccca aagaatatag tagtgaacct
     9541 caactgccaa actccaatta tgaacaaaca aatgaggaat gaactgagat tcataactcc
     9601 cacctgttaa gagttcatag aagttggtca taaagcctaa gacgcccgca atctggccac
     9661 caattccagc aacatagtct tggcgaacca agaaagtaaa aggcatggtc accaagacca
     9721 tcaaaaccac aggtggcaca atccgataaa agcgtcttct aaaaaatcct atcaaatcaa
     9781 tctcattgtt cttagaaaat tcttcaatga gtagagctgt aatcaggaaa cctgaaaatg
     9841 tgaaaaagac atctaccccg aaaaatcctc caggaaagat cgtctgaaag aagtgataca
     9901 agagtaccaa aagtaaacct ataatcctaa tcaaggaaaa ccatttaatg cgcatacgag
     9961 tttattcttc ctttcattgt acactttatt ctatcaaaaa aagaagaaaa atcctaagag
    10021 aaaacttagg atttttagct tatatcaatt ccattttaga aattacgtcc tgacttatta
    10081 tagccatatt tttccacaaa atacttacgg aattccaaga gattgtcatc catgatggct
    10141 tgtcgcactt gcttcatcag gttaagcaag aagtaaagat tgtggtagct agtcaagcgg
    10201 ataccaaagg tttcatcagc cttgagcagg tgacgaaggt aagcgcgtgt atagttatta
    10261 catgtgtagc aatcacactc aggatccagt ggcgtaaagt cctcagcaaa ctgggcattt
    10321 ttcacaacca aacgtccttg actggtcata caagtcccgt tacgagcaat tcgagtcggt
    10381 aagacacagt caaacatatc caccccacga atgaccccat cgatcaagct atctggcgct
    10441 cccacaccca tcagataacg aggtttattt tcaggcagca gttgagttgt aaagtccaag
    10501 accgcattca tctcttcatg ggtttctccc actgccaaac caccgataga gtagcctgag
    10561 aaatccatgc tgacaagatc gtgagctgat tggcggcgaa ggtcttcaaa tcctgcacct
    10621 tgcacaattc caaacaaacc ttggtcatgt ggacgacggt gagccttcaa accacactca
    10681 gcccaacggc tggtacgctc gatcgatttc ttaacgtagt cataaggttg ataaaactga
    10741 ggacattcat caaaggacat catgatgtct gaacccagat tattctgaat agagatggct
    10801 ttttctgggg ataggagcat cttagaacca tttagatgat ttttaaaggt tactccttct
    10861 tctgtgatat tacggctatc tgctaaagaa taaacctgaa aaccaccact atctgtcaag
    10921 ataggctggt cccaattcat gaacttgtgg agaccaccag cgcgtgcaat gagttcatct
    10981 ccagggcgaa gccagagatg ataggtgttt gataggataa ttcccgaacc catctccttc
    11041 aattcttcag gtgactgagt tttgacagtg gcttgtgtcc caactggcat aaacataggt
    11101 gtcggaaagg taccgtgggg agtgatgatt tctcctagac gagctcctgt gtgtttttct
    11161 ttcttaatca aacgatattt gattggtgaa tctgacattt tttacctccg aagctgggaa
    11221 agacagtccc agttcataca gtgtgcccaa ggacacactg tatgatttta tcaaaaaaag
    11281 taggaactgt cacgaactat gtataagaac tatggtataa taagagaatg aactatccaa
    11341 aaattgattt aaaaacaatt cgtcaggagt caaaacattt tcaggctgac accccccgtc
    11401 tctttctcct ctatatcctt cccagtatgc tggtcatctt atctggtttt cttaatccct
    11461 tatctcggat ccacgggact gttttagaac agccattttt cagcatactt ggccaaatac
    11521 ttcaaaccta cctctttcct ctactagtct cctttatcgg gactattctt ttgaccagtt
    11581 ccgtctatgc aacactgaca ctcatgaaag attctaaaac ggagccatct gtaaaaaaca
    11641 gcctagctct ctttgatgag gagcgctttt ctcaaacctt tttgacccta cttctcaaac
    11701 gtttctatct ctttttatgg agtatcccta atttacttgg gatttacctc ctcttttata
    11761 gtagcttttt agccaaaaaa ttcgtcaccc tacatcctga gtttccaaat ctagatctct
    11821 catcagttga aaccgagcgt ttcctcatgg tctttggact ctactttctt gcaagtctca
    11881 tcttgatcat tgtaggaaat atcctctata ttccacagta ttatgcctat tcacaggtgg
    11941 aatttcttct ctgttatagt ctagacctag gacaggtccc accaagacga atcctaaaaa
    12001 ctagccgttc ctttatgaag ggctacaagt tccagcactt tgtcctagat ttacagcttc
    12061 ttccttggta cttcctcaat tggattacct ttggcattgc tagtttttca ctcctacctt
    12121 atatccaatg cactaaaatt atgttttacc gagcagtatt ggctcgaaaa cgtccaaaag
    12181 cttgaaggtt tttcccctca agcttttttc tatcaatgag tttctccgcc taccaacttg
    12241 agttcctgat ctccatgttc tatcatagcg ccgattgctg cttctatccc tcgccgaata
    12301 tccactaaac tcatagttgg agtagtcggt ctgttcacca cctgttccat catataagga
    12361 atatgcataa aacctgcctt aacatatgga aatttctttt ctaccaaata gagagcctga
    12421 tacatcaaat ggctgcagac aaaagtccct gccgtattgg aaacagaggc cggtaagccc
    12481 tcttttttta tagcttgaac catcgcttta atcggcaaac tactaaagta ggccgaagca
    12541 ccatctgggc gaatgggacg gtcaatcggt tgattatctt cgttatcaga aatgcgtgca
    12601 tcgtcttgat taatggccac tcgttcaggt gtcacactag ttcttccacc agcttgccca
    12661 atacaaagga caaagtcagg ttgataacga ttcatctctt cttccaatac ttgagcagat
    12721 ttgtgaaaaa ctgtcggcac ctctagccaa cggacctcag caccatggat ttcagctggt
    12781 aaacctttaa tggcctccaa agttggattg cccttttccc ctccaaaggg ctcaaaacct
    12841 gtcactaata ctttcatttc taactcctca aaaccaattc aatgagactt ttttcttaaa
    12901 aacaagtata acatatttcc cttattctgg attaaaaagg attagcgaga ttaaaatcga
    12961 ccttcaagtg tatttaaaga gtaaaatact aatcaaggcc aaaatagctg atccaccttg
    13021 cttcaaaata atttttttat ccgctgttaa agagccgtaa gtcgcagcac caatcacaaa
    13081 taagacaaaa atagtcacaa tttctaaatt ctgtgagaaa taaatgccat accagagaaa
    13141 gactcctagc agagccttat aaatcccttg atttttgaac aatgaactta ctgacggatg
    13201 agccaattct tccttttcca tattaaatac acgactagtc gcatctgatc gcgtagcaat
    13261 actttccaaa taaaaaatgt aaaaatgctc caaagcaacg atcgttgtta aaataattgt
    13321 aataattgac atctatttct ccttaaattt ctatattttc aatacccaaa accaatttat
    13381 ttaataaacg gctgagttct gttctctcaa actctgttaa gatactttcc atctcttcct
    13441 taaccttgat atgttgcaga gggggattca ccactaactg ctccttggca tacttcgcag
    13501 cctctaccaa cacttcccgc tgattttcag gattacgatg acgctccacc aaaccttccg
    13561 tttccaaaat cttgaaatgc cgtgtcaaag cagcctgatc aattttcaaa cgctcctgaa
    13621 ccgccatttg gttacaagga gaatgctcca gcaaaaacag taaaatctga taccgtgtca
    13681 aactaatccc aagccttttt tcaaacaatt gcgtactcgc ttgctcagac aagcggagtt
    13741 gatacagtaa atcttgaatc tctatcattt cttcctccta tccattgact tatcaatagt
    13801 tgaccaatca attataatat aaagtatagt gaaatgaaat aagagcagga caaatcgatc
    13861 aggacagtca aatcgatttc taacaatgtt ttagaagcag ttgtgtacta ttctagtttc
    13921 aatctactat aaaatagatt gcaagaatgt tgcttagatt attacaagat gataactttc
    13981 cattttccat tcaaattgat actcaatgaa aatcaaaaag caaactagga agctagtcgc
    14041 aggttgctca aagcacagct ttgaggtttc agataaaact gacgtggttt gaagagattt
    14101 tcgaagagta tgacaaagaa aaaacgaacg agaatcgctt cccatcacga aagctagatc
    14161 tcattcgtca aaatgatgtt accaagtcgg gtttatccat tcattgaaac gtgaacgtga
    14221 tcatagtgat tttctgtcac actaccacgg tctggcattg ggttccaagt gttagctggc
    14281 ccatatttgc tatcgaatgg agcatagaaa cgttgtttcc agatgatgta actaatgcca
    14341 cggctggcca tgttttgaat agcatattcc gcaatcttat cccctaattc tgaacgttct
    14401 ggtaccataa agtcgatagc caaacctttt ccgtgatctc cactgtctcc tggacgataa
    14461 ccgctaaagg atgtaatgcc aaacaagtta gcaatttctt ctttaaaggc agctgtttgt
    14521 ggttgaagac ctgcattttc agattttgct actgcaagtc cagcataatc gggcgcagct
    14581 ggagcctcgt aagttgttga aattattttc gtctcttctg gttgataagt agaaactgct
    14641 gctgtagctc catttgatga tgctacttct tttgcttctg aacttgttgt agttgcttcc
    14701 actgctgatg cagcttgagg gcttgcttgt gtttcctgct tctcagctgg agtcgttccc
    14761 tgaggagctt cttctgcaac tgcactgctt gtttcggccg tttgctcctc tgggacagaa
    14821 gtgcccgtag atggtgccac ttcttcagaa gcaatcactg tctttgtaac ttctgaactt
    14881 gatgctactt cttttggagc ttctgcaatt ggttgagaaa ggtctgcaac ctgaacagtt
    14941 tgatcatcaa cggtcacttg attagtggtc aaatctgctg tcgcagttgt cacttcttca
    15001 ctagagtctg cttgaggtgt ttggatttca acttctgtta cttcttctgc ttcattgaca
    15061 gtcgttgtca aaacagtttc tgggaaaatc aagtccatat tagtgatttt gttcagattc
    15121 gcaagcactg tgacatctac acccaaggct tctgcaatgg tgctcaaagt atcaccatac
    15181 tgtacggtat aacttgtttt gttgtccgtt ttagtcaaat cgttttggat ttgctcaaca
    15241 ctacgtgcag tccaaagaac ttcttctgct tgagttgcca atactggggc aaatgacaag
    15301 gctactgttg acgctaataa cattcttttc ttcattcttt caaattcctt tcaaatgagt
    15361 acctgtctat cataacacaa aaaatgtaga aaaaaagccc tctcgggcct atttaacaga
    15421 actgtctatt ccttgtaaca aactagatta cttgaaatag tttgttaggt ctctgtcaca
    15481 aaactgacac aatcttttta tcacttagtc tcaaaaatac gggaaatatc tgctaacgct
    15541 tgaatcctgt gattcccttc ataagtagac tctaaaaagt tgatactttg aatcccacta
    15601 ttctgggcaa attccacatc cagagtccga tcccctatat aataagtatt atcagaattc
    15661 aactgatact tatctagcag ataggtagcc gcttctggac ttggcttccg cacaaagcca
    15721 ctctgactgg tcaaaatctc tgtaaaatag gattccaccc ccaagtcctt gagaatggta
    15781 aaagtgttgt tccccttatg agtatatata aactgctgaa ttcctgattc gtctgcccaa
    15841 gctagcacct cacgcgcacc tggcatcaaa actacctgag cattcttctc agccagactc
    15901 tgggcacgca cctgatttag cacctcaaca tccagatttc tatcttctgc cacccgcaca
    15961 agcaaatttt gcaccgaata cttgaagata aactctctca ccttctcctt atcataagga
    16021 atagaaaact gagcaaaagt ctcctcaatc cctgataaaa tcgcttcgta agagtccaat
    16081 aaagtcccgt ctaaatccca aataaaagct gttttttgca tttcctatcc tttcaagctc
    16141 atatagcgct ggtaacgata gcgtagaaat aaccagcgaa aaccattatc caagagagac
    16201 cctgcccaaa taccaggcaa gccccaacca agcacaatac ccatcagata tcctgtccca
    16261 atgcggatac accacatgcc tatacttgtc gcataaaaag ggaggcgtgc atttcctaat
    16321 ccctgccaga ctgccgtata gatgactgtt cctgtcgtca taggggtacc aagtagtgaa
    16381 aacagtgtca ctagaacact agcctccacc gctagagaat cagtcgtata gagatgagtt
    16441 aatggtacac ccaagacata tatactaaag gacaggggca acatgaggaa cagagaaagc
    16501 caaaaggttt gtttactcaa actagcaact cttttccaat catcctctcc aactgctcgg
    16561 gccaacagca tgaccgttgc cgtagcgaca ccaaaggcag gcatatagtt aaactgggtc
    16621 aagacttctc cgattgcatt cccagcaact gcctccgtcc caaaagaaac gaccaaggca
    16681 atgatcacta catctccagc cctcatcata agtcgctctc cagctgctgg taaagccaag
    16741 gtcaacagtt ccttatctaa accaaaagtt agcttcccat aaggcagttt taattgtgac
    16801 cacaaaatca caagaccaac caaacgagac agaattgtcc cccaagcaac accagctatc
    16861 cccatatcca gaacaaaaat agctagactt gaaaaaagaa tattcaaggc attggataaa
    16921 aaactaacat agagaggcag acgtggatta tgcgttgcac gaatcaaggc tcccaaacta
    16981 gtcattaaac ctaagagaac aatcgatccg cctaccaaag atagatacag tccaccactc
    17041 tcagctacat ccctctccgt ccccaaaagt cctatcatct ctttcccagc gaagatggac
    17101 aaaaatccta aaaggaaact taatagtaag gtaatcttca acgcctcagt cacatgatag
    17161 gccaacttcg actggtcttt ctgccctatg ctttttgaaa taacactgga aatagcagct
    17221 cccagagcga tgaaaatcgc ctgataaatg gtgataatat taccagctac tgaaacccct
    17281 gaaatagcta tcaatcctaa atgagcaacc aaataactgt ccaccattcc cattagcatc
    17341 tgcaaaaagt tttcacccat agctggcaat gcaatattaa gaatgtcttt atttttctta
    17401 aacaatctct cctcctgatg aaaagaaact cagttggttt cccaaccgag tttgctccct
    17461 ctgtcttaaa gtcctaaata agcctcaacc gctgcttgca tgtcagcagc tgccactgtt
    17521 gtcttgtgac gaattggagc tatttcaagc ccatcaactg ctggtggcac tgcaacgcct
    17581 gagatttcat gtaattgagc caaggcttca aagtctgtca aacctgcttt tccagttaca
    17641 gcttctactg caactactgg gaacttgtat ggactagctg ttgaagcaat cactgtctta
    17701 gttacatctc cagtaaccgc ttggtatttt ctataaactg ctgaggcaac cgccgtatgt
    17761 ggatcctcaa tataagaatc tgactcataa acacgcttga tttctgccgc tgtttcttcc
    17821 tcagtcgcat attcagctgc aaagaggtcc aaaatctctg catcaaagtc tgtcaactta
    17881 tattgtcctt gcgtgttcaa ggcattcata agttcagctg tcttttcagc attatttccc
    17941 aaaagatgga aaatcaagcg ctccaagttt gaagatacca agatatccat agatgggctg
    18001 gttgttacct taaactcacg ttttttgtca tagacacgtg ttttaaagaa gtctgtcaag
    18061 acattattgt catttgaagc acagattagc ttgccaactg gtaggccgat ttgtttggca
    18121 taaaaggcag ccaagatatt tccaaagttt cctgttggta ctgtgaagtt aaccttttca
    18181 ccagctacaa tttcaccagt cttaaccaat tgagcgtaag cataaacata ataaacaatt
    18241 tgtggcacca gacgaccaat gttcatagag ttagctgatg aaaattgcaa cttgttggta
    18301 gtcaattttt cacgaagagc cacgtcgtta aacatgtgct tcacatttgt ttgcgcatcg
    18361 tcaaaattac catcaatagc aataacatga gtattgtcac cattctgagt ggtcatttgc
    18421 aactcttgta ccttgctgac accacccttt ggataaaaga cgataatctc agtaccaggc
    18481 acatccgcaa accccgccat agcagctttc cccgtgtcac cagatgtcgc tgtcaagata
    18541 acaatcttgt tctccaaacc atgtttctta gcagcagtcg tcataaagta tggcaaaata
    18601 gacaaggcca tatccttaaa ggcaatcgtt gaaccatgga aaagttccaa attgtattgc
    18661 ccatctaatt tcactaatgg tgcaatagct ggagtatcaa atttgctatc gtaggcattg
    18721 ttgatacagt agtccaactc ctcagctgta aagtcatcta aaaatgctga caaaactagc
    18781 ttagcaactt cctggtaaga agcatctttc aatttgtcaa agtccaaatc tacctttgga
    18841 taagtaaccg gtgtaaacaa accgccgtcc gtcgccaaac cttgcaaaat tgcttggctg
    18901 gcagttactg tattgttggc atcacgcgtt gattgataaa ctaatgtcat tactctctat
    18961 cctttatcta tagtctcgtc cattatatca tgatttttca gaaaattctc cctttataag
    19021 agaaattata ggctcaattc ttctctcaaa ggctgtaaat aggtcatttc ttccttattt
    19081 ctcaccacta gtccctcctc agctagtagg agtaagtctt tggaaaattc aataatcgta
    19141 gtttcttcct catctgtaag atttttctta gaaaattgtc tccttaaaga cttgtaatca
    19201 taaccaaata ctttaaagaa aggtgctgtt tctaagtaag cttctaactt gtctaaatta
    19261 accaataatc ccaagtgaaa agctgcagaa gcaaaagtcc tatcaagtgg ctgtgtacac
    19321 acactacgaa actcaactgt tcctcgagtc gttaaatctt ggtactggta actacgatga
    19381 gtttcaaaat ccttctcttg ggggtaaata ataacctcat ccccattcag agcaaatgct
    19441 tggatttcgg acgtagccaa atagtcccca gcctgaatag gataaaaata ataggtctgc
    19501 ccatcacgtt ccgcagtaaa aatcgcagaa tgatttagat agtcaaaaaa atcagcttca
    19561 tcattaagga gtctagcatt gaccccaaca ttctctggat agataccatg catagattct
    19621 tcccagaaaa tatcccttga aattttcgta tcccaatccg cacccgaaaa ttcagagttt
    19681 gcaaataaat aagccttagc cgcttcaatt tgagtaaaag cattaatcac ccgtaagtag
    19741 ttggtttttg aaatatccag ctgaacctgg ctcccacaga taaaagtacc atattcaggg
    19801 aaatgatgta aatctgattt aataatattt ctactcaaat tcaaataatc catcaacatc
    19861 tgatagcgtg gataagccac tggacaattc tcatttttat cccagttggg atggatacca
    19921 cagccaacaa tagcatgatt tgattcagct aactttctct gaattacatt catataatta
    19981 ttgaaacgat tttcgacctc ttgaatcgtt tcagccttac caaatgcaaa ctcaatcgtt
    20041 gtataggaaa cttcaaataa aatagcatcc tgacttatcg gatctactaa ctggatcaga
    20101 ttgccaaaat catctacctt tgctacggtg agatccaaag tagaaactaa ataatgaaat
    20161 agatccttta taacttcaac atctgtagca tccccttcta aacttgcaac aggatactcc
    20221 aactcaattc cgacaaacaa ttcaggattt tctttaatat ttttcaaata ccgatgtttt
    20281 aataaatcga tagaacaaga catactaccc tacactttct taatctaaat aaaatttgaa
    20341 taactataat tataccaaaa ataatataaa aaaggaacga agaataacaa cgttcctaac
    20401 actatactat accggcggcc ggggtcgaac cggcacgtcc ttgcggacac tggattttga
    20461 gtccagcgcg tctgccaatt ccgccacgcc ggcaaatagt aactggggta gctggattcg
    20521 aaccaacgca tgagggagtc aaagtccctt gccttaccgc ttggctatac cccaataata
    20581 taaaataggc gagtgatggg gatcgaaccc acgcatgcca gagccacaat ctggtgtgtt
    20641 aaccacttca ccacacccgc cataattcta ttaacacggg cagtaggaat tgaacccaca
    20701 ctgaaggttt tggagacctt agttctacct ttaaactatg cccgtaaaat ggaaggggag
    20761 ggattcgaac ccccgaaccc gaaggagcgg atttacagtc cgccgcgttt agcctcttcg
    20821 ctacccttcc aaaatatata aatggcgcga gacggaatcg aaccgccgac acatggagct
    20881 tcaatccatt gctctaccaa ctgagctacc gagccttatt gcgggagcag gatttgaacc
    20941 tacgaccttc gggttatgag cccgacgagc taccgagctg ctccatcccg cgttaataat
    21001 ataaaaggag gatgtgggat tcgaacccac gcacgctttt acacgcctga cggttttcaa
    21061 gaccgttccc
//
LOCUS       DAVBBS010000037        20780 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVBBS010000037 DAVBBS010000000
VERSION     DAVBBS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20780
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.037"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            398..1091
                     /locus_tag="VQU32_001351"
                     /pseudo
     CDS             398..1091
                     /locus_tag="VQU32_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1147..1419
                     /locus_tag="VQU32_001352"
     CDS             1147..1419
                     /locus_tag="VQU32_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HET2472793.1"
                     /translation="MFLLFSALVGMLSQLKEVPESVFLQETVEENHLVNVYSVLEVIS
                     TLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK"
     gene            1733..1864
                     /locus_tag="VQU32_001353"
     CDS             1733..1864
                     /locus_tag="VQU32_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HET2472794.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            1885..3435
                     /gene="dltA"
                     /locus_tag="VQU32_001354"
     CDS             1885..3435
                     /gene="dltA"
                     /locus_tag="VQU32_001354"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HET2472795.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFVQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMIMDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGVTHFYFDG
                     EELTVKTAQKLRKRFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            3432..4676
                     /gene="dltB"
                     /locus_tag="VQU32_001355"
     CDS             3432..4676
                     /gene="dltB"
                     /locus_tag="VQU32_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HET2472796.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            4690..4929
                     /gene="dltC"
                     /locus_tag="VQU32_001356"
     CDS             4690..4929
                     /gene="dltC"
                     /locus_tag="VQU32_001356"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HET2472797.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            4922..6190
                     /gene="dltD"
                     /locus_tag="VQU32_001357"
     CDS             4922..6190
                     /gene="dltD"
                     /locus_tag="VQU32_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HET2472798.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            6345..6785
                     /gene="adcR"
                     /locus_tag="VQU32_001358"
     CDS             6345..6785
                     /gene="adcR"
                     /locus_tag="VQU32_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HET2472799.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            6785..7489
                     /locus_tag="VQU32_001359"
     CDS             6785..7489
                     /locus_tag="VQU32_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET2472800.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNTHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            7482..8288
                     /locus_tag="VQU32_001360"
     CDS             7482..8288
                     /locus_tag="VQU32_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET2472801.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGRSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            8298..9803
                     /gene="adcA"
                     /locus_tag="VQU32_001361"
     CDS             8298..9803
                     /gene="adcA"
                     /locus_tag="VQU32_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HET2472802.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKVTFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(10923..11696)
                     /locus_tag="VQU32_001362"
     CDS             complement(10923..11696)
                     /locus_tag="VQU32_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2472803.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTLPDQKLLDAVELFDGEWVSDIQ"
     gene            11872..13275
                     /locus_tag="VQU32_001363"
     CDS             11872..13275
                     /locus_tag="VQU32_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HET2472804.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEATAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            13390..14028
                     /locus_tag="VQU32_001364"
     CDS             13390..14028
                     /locus_tag="VQU32_001364"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HET2472805.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMELMAEKFKTYGQRK"
     gene            14040..14480
                     /locus_tag="VQU32_001365"
     CDS             14040..14480
                     /locus_tag="VQU32_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HET2472806.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            14473..14904
                     /locus_tag="VQU32_001366"
     CDS             14473..14904
                     /locus_tag="VQU32_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2472807.1"
                     /translation="MFNRGILVASHGDFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVDELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISNYDSKINLEEIVHNAQNSLFNVKQQLNVEEEENLCL"
     gene            14895..15365
                     /locus_tag="VQU32_001367"
     CDS             14895..15365
                     /locus_tag="VQU32_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2472808.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            15390..16163
                     /locus_tag="VQU32_001368"
     CDS             15390..16163
                     /locus_tag="VQU32_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2472809.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            16160..16960
                     /locus_tag="VQU32_001369"
     CDS             16160..16960
                     /locus_tag="VQU32_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2472810.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            17019..19313
                     /locus_tag="VQU32_001370"
     CDS             17019..19313
                     /locus_tag="VQU32_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HET2472811.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPVNLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            19323..>20780
                     /locus_tag="VQU32_001371"
     CDS             19323..>20780
                     /locus_tag="VQU32_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472812.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLH"
BASE COUNT         6450 a         3391 c         4377 g         6562 t
ORIGIN      
        1 ctactcgatt aactcccctg aaatttgttt taattatgca aatttcacgt atttttgatg
       61 ctgagacgac gatcctggga acttttcaga tatttttttg actatctaaa atctatcatt
      121 agaaaagctt agagcgccaa aggatttgag cgtttttctg atttttaaga ctttttccca
      181 gtctcttttt cgattgaaga tgtaattatt ctactaacta acttcttagt actagccaac
      241 aacgataatc ataattcctc ctaaaattag gaataataaa ggcaatagtt tttgtttttt
      301 catgtaaaaa acctcacttt tgttttctgc tattttatgc taaaatatta aaaatcaaat
      361 ttaattccaa agtttgtaac taaaggggga gcgctacatg tctaattcat ttgtcaagtt
      421 gttagtctct caattatttg caaatttagc agatattttc tttagagtaa caatcattgc
      481 taacatatac attatttcaa aatcagtaat tgccacatca ctagttccta tcttaatagg
      541 aatatcctct tttgttgcga gtcttttagt tccgttggtt actaaaaggt tagcgctaaa
      601 tagggtttta tctttatctc aatttggaaa gactatatta ttggcgatac tggtaggaat
      661 gtttaccgta atgcaatccg tagcgccttt ggtgacctat ctatttgttg ttgcaatttc
      721 catactagat ggttttgcag cacccgtttc ctatgctatt gtgccacgct atgcgaccga
      781 tttgggtaag gctaattcag ccttatcaat gactggtgaa gctgttcaat tgataggttg
      841 gggattaggt ggactcttgt ttgcaacaat tggtctgtta cctaccacgt ttatcatttt
      901 agtcttgtat atcatttcta gctttctgat gttatttctt cctaacgctg aagtggaggt
      961 gttagagtca gaaactaatc ttgaaatttt gctcaaaggt tggaagttag ttgctagaaa
     1021 tcctagatta agactttttg tatcagcaaa tttattggaa attttttcaa atacgatttg
     1081 ggtttcttcc attttgtagc tatggcaata gtgacactga ctattctata ttttccaaac
     1141 gcacagatgt ttttattatt ttcagcttta gttggtatgt tatcgcaact aaaagaagtt
     1201 cctgaaagtg tatttcttca ggaaacagta gaggaaaatc atttagttaa tgtctattcc
     1261 gttcttgaag tgatttctac attagcattt tcagtatttg ttttgctaat gagctatatt
     1321 actgagagtt ttggtattag catcagtttt tggctatcag ccatttgtct gatgatagaa
     1381 gctattttaa tttatatcag acgagattat tttaaatgag agagtggtta aaaagtcttt
     1441 aaaaatcaga aaagcgcata gtatcaggtg ttgaaaaaac ttgatattgt gtgttttatt
     1501 atgggaagat ttacttcatt gtctcctgaa atggagtttt gaagcaggat cactctattt
     1561 gaaaatatac atagaaaaac tgtataatcc ttaaaataag ctgaaatcta ttggaaacat
     1621 acggtaaaca attgaaaagg tactatataa agtggtacaa tggtagaaaa ataagctagt
     1681 aagaatcact cttattttag cgaaaaatta cagaaatgaa tggtttttct tgatgaaaca
     1741 gagaaaagaa ttgtacctct ttcttggtcg gacagccttg tattttctta tctttctagg
     1801 gctgctttac ttctttagct atcttggtca gggtcaagga agctttatct ataatgaatt
     1861 ttaacttgaa ggagaatccc tattgtgtca aataaaccaa tagcagatat gattgaaacc
     1921 attgagcatt ttgctcagac acagcctagc tatcctgtct ataatgtttt ggggcaggaa
     1981 cacacttatg gcgatttaaa ggctgattcg gatagtttgg ctgcagtcat tgaccaacta
     2041 ggcttgcctg agaagtctcc tgtggttgtt tttggtggcc aagaatatga aatgttggca
     2101 acctttgtag cgctgactaa gtcaggtcat gcctacattc caattgatag ccattcggcc
     2161 ttggagcgag tttcagctat tttagaagta gcagagccaa gcttgattat tgccatttca
     2221 gcctttccct tggagcaggt ttctacacca atgataaatc tagctcaggt tcaagaagcc
     2281 tttgttcaag ggaataacta tgaaatcacg catccagtca agggagatga taattactac
     2341 attatcttta cttctggtac gactggtaag cctaagggag tgcagatttc acatgataat
     2401 ctcctcagct ttacaaactg gatgattatg gataaggaat ttgcgacacc gagtcgtccg
     2461 caaatgctgg cacagccacc ttattctttt gacttgtctg tcatgtattg ggcaccgacc
     2521 ttggcacttg gtggtacgct tttcactctt ccttcagtca tcactcagga ctttaagcaa
     2581 ctctttgcgg ctatcttttc attgccaatc gctatctgga catcaacacc atcctttgca
     2641 gatatggcca tgttgtctga atacttcaac agtgagaaaa tgcctggagt cacgcatttc
     2701 tactttgatg gtgaagaatt gacggtcaaa acagctcaaa aactgcgcaa gcgtttccca
     2761 aatgcccgta tcatcaatgc ttacggccca acagaagcga cagtagctct gtcagcagtt
     2821 gccgtgacag acgagatgtt agcgactctc aaacgcctac caatcggcta taccaaggct
     2881 gattctccaa cctttatcat tgacgaggaa ggaaataaac tgccaaatgg cgaacaggga
     2941 gaaatcattg tttctgggcc agctgtttca aaaggttata tgaacaatcc tgaaaaaaca
     3001 gcagaagcct tctttgagtt tgaaggtctg ccagcctatc acacaggcga tgtgggaacc
     3061 atgacagatg agggcttgct tctctacggc ggacgcatgg acttccagat taagtttaac
     3121 ggttaccgca ttgagttaga agatgtctct caaaacctca acaagtctcg ctttatcgaa
     3181 tctgctgtcg cagtaccgcg ctataacaag gaccacaagg tacaaaatct attggcttat
     3241 gtcatcttaa aagacggtgt tcgtgagcag tttgagcgag atatcgatat taccaaggcc
     3301 atcaaggaag acctgacaga catcatgatg tcctatatga tgccatctaa attcctttac
     3361 cgagacagtt tgccactgac tccaaatgga aagattgaca tcaaaggatt gattaacgag
     3421 gtgaataaga gatgatggag ttttttcaac agcttcctca tttagagcca tatggcaatc
     3481 ctcagtattt tgtttatgtg attgctgcaa ccttgcccat ctttataggt ctctttttca
     3541 agaaacgctt tgcctggtat gaagtgttgg taagtctctt ctttattgtc accatgttgg
     3601 tgggtggaaa gaccaatcaa ctagctgcct tgggtattta cctttgctgg gaaatattgc
     3661 tcctgctttt ctacaagcat tatcgaaaaa gcaaggatgg caagtgggtc ttctacttag
     3721 ttagttttct gtccctactt ccgattatct ttgtcaaggt gcaaccagct atcaatggaa
     3781 cgcagtcttt gcttgggttc ttgggaattt cttatcttac ctttcgttcg gttgggatta
     3841 tcatcgagct gagagatgga gtgattaagg attttaccct ctgggaattc ctccgtttcc
     3901 ttctcttcat gccaactttc tcgagtggtc caatcgatcg ctttaagcga tttaatgaaa
     3961 attatcaggc tattcctgag cgagatgagt tgatggatat gctggatgaa tctgtccgct
     4021 atatcatgtg gggctttttg tataagttta tcctagctca tgttttagga gagaccttac
     4081 tacctcctct gaagaattta gccttgcagt caggtggctt ctttaatctc tatgccttgg
     4141 cagttatgta tacttttggt ctggagctct tctttgactt tgcaggttat tctatgtttg
     4201 ctttggccat ctcaaacttg atgggaatcc gtagccctat caactttaac aagccctttt
     4261 tatcaaggga tttaaaggag ttttggaatc gctggcatat gagtctgtcc ttctggttcc
     4321 gtgactttgt ctttatgcga atggtgatgg tgttaaccag aaagaaagtc tttaaaaatc
     4381 gtaatgtaac ctcaagcatg gcctacattg taaatatgct gattatggga ttttggcatg
     4441 gtgtgacctg gtactatatc gcctatggac tctttcatgg actaggcttg gtcatcaatg
     4501 atgcctgggt tcgcaagaaa aaaacactca ataaggaacg gaaaaaagca gggaaggctg
     4561 ccctacctga gaatcgctgg attcagttgc ttggcatggt tgtcactttc catgttgtca
     4621 tgttgtcatt cttaatcttt tctggattct tgaataatct atggtttaaa aaataaaagg
     4681 aaataaaata tggatatcaa atcagaagtt atcgaaatta ttgatgagtt gtttatggaa
     4741 gatgtttctg acatgatgga tgaagatctt tttgatgcag gtgtcttgga tagtatggga
     4801 acggttgagt tgattgtgga gattgagaac cgttttgaca ttcgtgtccc tgtaacagag
     4861 tttggtcgtg acgactggaa tacagctaat aaaatcatag ctggtattgt ggagctacaa
     4921 aatgcttaaa cgcttatgga tgatcttcgg accggtcttg atcgctggtt tgttggtttt
     4981 tctgctcatt ttcttttatc ctactgagat gcatcataat ctaggagctg aaaagcgttc
     5041 agcagtggct actactatcg atagttttaa ggagcgaagt caaaaagtca gagcactatc
     5101 tgatccaaat gtgcgttttg ttcccttctt tggctctagt gaatggcttc gttttgacgg
     5161 tgctcatcct gcggtattag ctgagaaata caatcgttcc taccgtcctt atcttttagg
     5221 acagggggga gctgcatcgc ttaaccaata ttttggaatg caacagatgt taccacagct
     5281 ggagaataaa caagttgtgt atgttatctc acctcagtgg ttcagtaaaa atggctatga
     5341 tccagcagcc ttccagcagt attttaatgg agaccagttg actagttttc tgaaacatca
     5401 atctggggat caggctagtc aatatgcagc gactcgctta ctgcaacagt tcccaaacgt
     5461 agctatgaag gacctggttc agaagttggc aagtaaagaa gaattgtcga cagcagacaa
     5521 tgaaatgatt gaattattgg ctcgttttaa tgaacgccaa gcttcctttt ttggtcagtt
     5581 ttcggttaga ggctatgtta actacgataa gcatgtagct aagtatttaa aaatcttgcc
     5641 agaccagttt tcttatcagg caatagaaga tgttgtcaaa gcagatgctg aaaaaaatac
     5701 ttccaataat gagatgggaa tggaaaatta tttctataat gagcagatca agaaggattt
     5761 gaagaaatta aaggattctc agaaaagctt tacctatctc aagtcgccag agtataatga
     5821 cttgcagttg gttttaacac agttttctaa atctaaggta aacccgattt ttatcattcc
     5881 acctgttaat aaaaaatgga tggactatgc tggtctacga gaggatatgt accaacaaac
     5941 ggtgcagaag attcgctacc agttagaaag tcaaggtttt accaatatag cagatttttc
     6001 taaggacggc ggggagcctt tctttatgaa ggacaccatt caccttggtt ggttgggttg
     6061 gttggctttt gacaaggcag ttgatccttt cctatccaat cccacaccag ctccgactta
     6121 ccatctgaat gagcgctttt tcagcaaaga ttgggcgact tatgatggag atgtcaaaga
     6181 atttcaatag ataatataat atagaagagt tcaagaatga aactagtttt cacgtttttt
     6241 cttgactctt tttttgcttg tgatataatt aactggtaaa caaaatgtca aagaatagta
     6301 tttttctctt aaaaaagaga agtctaaaag gaaaggagtc agatatgaga cagctagcaa
     6361 aggatatcaa tgcttttttg aatgaggtga ttttgcaggc ggaaaatcag catgaaatcc
     6421 taataggtca ttgcactagc gaggtggccc tgaccaatac tcaggagcat atccttatgc
     6481 tcttgtcaga ggaatcttta acaaattcag aattggcccg tcgtctcaat gtcagtcagg
     6541 cggcagttac caaggccatt aagtctttgg tcaaggaagg gatgttggaa acatctaaag
     6601 attctaaaga tgcgcgtgtg attttttatc agttgactga cttggctcgt ccaatcgctg
     6661 aggagcacca ccatcaccat gagcatacac ttttaaccta tgaacaagtg gcgactcagt
     6721 ttactccaaa tgaacaaaaa gtgattcagc ggtttttgac tgctttagta ggagaaatca
     6781 aataatgaga tatattacgg tagaggattt gtccttctat tatgataagg aacctgttct
     6841 tgaacatatc aattattgtg ttgatagtgg ggaatttgtt accttgactg gggaaaatgg
     6901 agcggctaag acgacgctca tcaaggccag tcttggaatt ctgcaaccac gcattggaaa
     6961 ggtggctatt tcaaagacaa atacgcaagg taagaaattg agaatagcct atcttcctca
     7021 acaaattgcc agttttaatg ctggttttcc aagtacggtc tatgaatttg tcaagtcggg
     7081 tcgctatccg agaaaaggct ggttccgtcg tttgaatact catgatgagg agcatatcaa
     7141 ggctagtctg gactcagttg gcatgtggga acatcgagac aaacgcttgg ggtctctatc
     7201 tgggggacaa aagcagcgag cggtaattgc gcgtatgttt gcttctgacc ctgatgtgtt
     7261 tatcctagac gagccgacaa cggggatgga tgcaggaagt aaaaatgaat tttacgaact
     7321 catgcaccac agcgcccatc atcatggcaa ggctgttttg atgattaccc atgaccctga
     7381 agaagttaag gattatgcgg atcgcaatat tcatctagtc cgtaaccaag actcgccatg
     7441 gcgttgtttc aacgttcatg agaatgggca ggaggtgggc catgcttagt ttattatctt
     7501 acgactttat acaacgcgcc tttttggcgg ttattgctat gagtcttttc tcaccggtat
     7561 tgggaacctt cctcatcttg cgtcgtcaga gtttgatgag tgataccctt agccacgtct
     7621 cactttcagg tgtagccttt ggtctggttt tggggatttc tccaactgtt tctactattg
     7681 ccattgtctt gattgcggcg gtctttctgg agtatctccg tacggtttac aagagcttta
     7741 tggaaatcgg gacagctatc ctcatgtcaa caggtttggc tgtttctctg attgtcatga
     7801 gcaagggtag aagctcgagt tcaatgagtt tggaccaata tctctttggt tcgatcgtga
     7861 ctatcagtga agaacaggtc atttccctct ttgtcattgc ggcggttgtt ttgattttga
     7921 cctttctctt tcttcgtcct atgtatatct taacttttga cgaagatacg gcctttgtgg
     7981 atggcttgcc agttcgtacc atgtccattc tttttaacat ggtgacaggg gtggctattg
     8041 cccttatgat tcctgcagca ggagctcttc tggtatcgac cattatggtc ttgccagcta
     8101 gtattgccct gcgtctgggg aaaaacttta aatcggttat gctgcttgcc agtgcgattg
     8161 gctttttggg aatggtagca ggactttaca tttcctacta tgcagaaaca cctgcaagtg
     8221 caagtattac cattattttt gtaactgtct ttatactaat cagtttagta agacgtttta
     8281 tcaaatagga gacgaacgtg aaaaaaatta gcttattact agccagtcta tgtgccttgt
     8341 ttttagtggc ttgttccaat caaaaacagg cagatggtaa actcaatatc gtgacaacct
     8401 tttaccctgt ctatgaattt accaagcaag tcgcaggaga tacggctaat gtagaactcc
     8461 taatcggtgc tgggacagaa cctcatgaat acgaaccatc tgccaaggca gttgccaaaa
     8521 tccaagatgc agataccttc gtttatgaaa atgaaaacat ggaaacatgg gtacctaaat
     8581 tgctagatac cttggataag aaaaaagtga aaaccatcaa ggcgacaggc gatatgttgc
     8641 tcttgccagg tggcgaggaa gaagagggag accatgacca tggagaagaa ggtcatcacc
     8701 atgagtttga cccccatgtt tggttatcac cagttcgtgc cattaaacta gtagagcaca
     8761 tccgcgacag cttgtcagca gattatcctg ataaaaaagt gacctttgag aagaatgcag
     8821 ctgcctatat cgaaaaattg caagccttgg ataaggctta cgcagaaggc ttgtctcaag
     8881 caaaacaaaa gagctttgtg actcaacacg cagcctttaa ctatcttgcc ttggactatg
     8941 gactcaaaca agtcgcaatc tcaggacttt ctccagatgc agagccatca gctgctcgct
     9001 tggcagaatt gacagagtat gtcaagaaaa ataaaatcgc ctatatctat tttgaagaaa
     9061 atgcctcaca agcccttgct aacacacttt caaaagaagc aggtgtcaaa actgatgtcc
     9121 tcaatccttt agaaagtctg acagaagagg acaccaaggc tggagaaaac tacatttccg
     9181 tgatggagaa aaacctcaag gctttgaaac aaacaacaga ccaagaaggc ccagcaatcg
     9241 aacctgaaaa ggcagaggat accaagacag tccaaaatgg ttacttcgag gatgcagctg
     9301 tcaaggaccg caccttgagt gactatgcag gtaactggca atcagtttat cctttccttg
     9361 aagacggcac gtttgaccaa gtctttgact acaaggctaa gttgactggt aagatgaccc
     9421 aggctgagta caaggcttac tatacaaaag gctatcagac agatgtgact aagattaaca
     9481 ttactgataa tactatggaa tttgttcaag gtggacaaag caagaaatac acttacaagt
     9541 atgtcggtaa gaaaattttg acttacaaga aaggcaatcg tggcgtgcgt ttcctctttg
     9601 aagccacaga tgctgacgct ggacaattca agtatgttca gtttagtgat cacaatgttg
     9661 ccccagttaa ggcagaacat ttccatatct tctttggagg cacaagccaa gaagccctct
     9721 ttgaagaaat ggacaactgg ccaacctact acccagataa cctatctggc caagaaatcg
     9781 cccaagaaat gttggcgcat tgatgagaaa tcgactagtt cataagagct agtcggtttt
     9841 ttgatactag atggctaact tcattataga cctttcagaa ctttaaacaa taaataaaac
     9901 tcttgaaata tctgggctta tatgctagaa tgtagttagt gtataaggtt cattagaaca
     9961 ccaaagcccc taactagtgc catagttaag ggcttttttg acgtatctta gcgatttaac
    10021 gggtgtttat aggctagtca gtcggtctac ttcttaccat ctagccattt gcagacgaaa
    10081 tacaagataa ttccagctat aacatctgct ataatagtaa gagcaagctc tggtataagg
    10141 ctcattagtt tcacctcctc tcacgaaccc ataggaacgt aatcggtaac cgatgacaaa
    10201 aatagtatac cacactaaat ttagatcatc aacctcactt aatgcttgaa ctattgtaga
    10261 ttatatgcta aaatgtagtt aattgataag gttcattaga acaccaaagc ccctaactat
    10321 ggccgtagtt aagggctttt ttgacgtatc ttagcgattt aacgggtgtt tataggctag
    10381 tcactcggtc tactccttac tatctagcca tctgcagacg aaatacaaga taattccagc
    10441 tataacatcc gctataatag taagagcgag ctctatgata aggctcatta gtttcacctc
    10501 ctctcacgaa cccataggaa cgtaatcggt aaccgatgac aaaaatagta taccacaata
    10561 tatttagatc atcaaggtca cttaattctt gaaatatagg gtctaggcga caaagtcttt
    10621 aaaatcagaa aaacgcataa tatcaggtgt gcaaaaactt gatactatgc gttttattgt
    10681 gggaaggttt actccatttt ctcctgaaat tgagtttttg tccagcctct gtttttagaa
    10741 ttgctaagaa aataatgtca tgtggtgaat atttgtaaat cagtcagcag acagaacgaa
    10801 tactcttcga aaatctcttc acatcatgtc agcttcgtct tgccgtgtcc atgggggact
    10861 tgacttgatc agttttcaaa tctgtacttt gagcaagctg agactagctt cctatttgat
    10921 tttcattgaa tatcagaaac ccattctcca tcaaataatt cgactgcgtc taataatttt
    10981 tggtctggca aggtgtcaga aataaaggtt gtgtatttgg agaggggatt aattttaaaa
    11041 aatccagtct tgtaaaattt agaactatca atcagtaaga tggtttcatg ggctttgtca
    11101 ataatattct tttttgaaat agcttggctg agagaagctt cataaacata ttggtcatca
    11161 atacctcttg ctgaacaaaa tgctaaatcg atattaaaat gatctaataa agaattttcc
    11221 ttatcatagt tgaccacgga acaggattga tgtttgacct cgccagatgt gataaagatt
    11281 ttggagctat ctttaacagt ttcagatagg gtttgtgcag tatgtaaacc atttgtaaaa
    11341 ataatcaaat tatcaagttc agaaagatag ggacaaagtt cgtagacagt agtactagaa
    11401 tctagataga tacacatacc agaccgaata aagtctttag cgagactagc gattagtctt
    11461 ttttgcctag tactttctcc ttcccgtatt tgatgagaaa gttcaattgt gttcatagag
    11521 gacagggtca cgtatccgtg ctttcttttg ataagacctt gattttctaa gaaaattaaa
    11581 tcacgacgta aggtacttgt gctggagaaa gtgatttctg ccagctcttt tacggcaatt
    11641 ctttttttct ttttaataat ttcaatcaat tcaagtacac gttcatcttt tatcataagc
    11701 tcctcctaat ttatcatttc aactatatta tagcacaaat tggaggaatt tgaattattt
    11761 ttatgaatat tgggttaaca tttgaacatt attcaagtaa gcgttcacat attgaaaaaa
    11821 taaaacgtgg ggattataat aaagttaatc aaggacgaag agagaagaaa aatggaagcg
    11881 gttttagcaa tagatttagg tgcgacttct ggaagagcaa tcgttggtta cctttctgaa
    11941 aataaactag taatggaaga aataaatcgc ttttctaatc tacctattag agtaaaaggg
    12001 catttatctt gggatattga ctttctacta gctaaaattc ttgaaagtat ccgcttggct
    12061 aatactagtt acaagatttt atctatcggt attgacacat ggggagttga ttttggactg
    12121 attgataatg aaggtaagct gttatcacaa cctgttcatt atcgtgatga aagaacaaag
    12181 ggagtgttaa aggaaatatc tgaaatgact gaattagaaa aactgtattc agagacagga
    12241 aatcagatta tggagataaa taccttgttt caactcttta aggcacgtca agaatctcct
    12301 gactctttct ataagaccaa taagattctt ttaatgccag atttgtttaa ttatctcttg
    12361 acaggtaagt ttgctacaga aaaaagcatt gcttcaacaa ctcaattatt tgatcctagg
    12421 agtcaaaatt ggaatcagaa tatcttaaaa ctatttgaat tggattcatc tttacttcct
    12481 gaaattgttt cagagggaaa tgttcttgga aggataaaaa aggagtatgg tttaggcgat
    12541 attcctgttg tgaatgtttg tagtcatgat acagcaagtg cgattgtctc agtacctaag
    12601 acagaaggta gtttatttat ttcatcaggt acttggtctt tggttggagt ggaacttact
    12661 tcaccgattc ttactaccga atccttcagt tatggattta caaatgaagt cggtaaagat
    12721 ggagtgatta catttctgaa gaattgtaca gggttgtgga tcatagagga actaagacgt
    12781 tcatttgaac gaagagggaa agcctattct tttgatgata ttaggacaat ggtggagaaa
    12841 gaaaaagaaa atcttcctct gattgatact gaatcaactg aatttgcaac agaatctgat
    12901 atgcacaaga ctttgacaga atatctagct tatcatcatg aaactagaga gtggacagat
    12961 ggacaactat ttaagattgt ttatgaaagc ctagctgaaa cgtataggaa agcgatagag
    13021 ttattagaag aactaactca taaggtttat aagaggatat atgtgattgg aggaggtgct
    13081 agagccagtt attttaacca aatgattgct gatagaactg gtaaagaggt tcttacaggt
    13141 ttgactgagg ctacagctgt ggggaatatt gttgtgcagc tcatagctat gggacaatta
    13201 aaagggatgg aagaggctca ccatgttatt gaggagtttc tacaattaga gagttattac
    13261 tcccaaaaga attaaaaaga ttgagagttt gtaaatttgc ctccctcccc cttcttagct
    13321 tttgtgcagg aaggggggat aattggtgaa ttgaaaaata tttagtgttt tgatatgagg
    13381 aggacaagga tgtcagatgt aaaacaagaa ttaattaaat atggtaagaa gctagtagaa
    13441 acagatttga cgaaaggaac aggtgggaat ctcagcgttt tcgatcgtga aaaacaattg
    13501 atggcaatta ccccgtcggg tattgatttc tttgaaatca aagaatccga tattgtagtg
    13561 atggatatta atggaaatgt tgtagaggga gaacgcttgc catctagcga atggtatatg
    13621 catttgattc aatatcaaac tcgtgatgat atcgatgcaa ttatccatgc tcatacaact
    13681 tatgcaacag tattagcttg tctcagagaa ccacttccag cgagtcatta tatgattgca
    13741 gtggcaggga aagatgttcg ggtagctgag tatgcaacat atggcacgaa agaattggct
    13801 gtgaatgcag ctaaagcaat ggaaggtcgt agagcagttt tactagcgaa tcatggaatt
    13861 ttagcaggtg cacaaaattt attgaatgca tttaatattg ttgaagaagt tgaatattgt
    13921 gcaaaaattt attgtttagc taagaatttt ggagagccag tagttcttcc tgatgaggag
    13981 atggaattga tggcagaaaa atttaaaaca tacggtcaga gaaaataggg aggatattaa
    14041 tgttaaaaca tataccgaaa aatatttctc cagatttatt gaagacttta atggaaatgg
    14101 gacatggaga tgaaatagta ttagctgacg cgaattatcc ttctgcctca tgtgcaaata
    14161 agctaattcg ttgtgatggc gtaaatattc cagaattatt agattccatt ctgtatttaa
    14221 tgccattaga tagttacgtc gatagttcaa ttcagtttat gaacgttgtt tcgggtgatg
    14281 atattcctaa gatatggggt acctatagac agatgattga aggtcatggt acagatctta
    14341 aaacgattac ttatcttaga agagaagact tttatgaacg tagtaagaaa gcttatgcta
    14401 ttgttgctac aggagaaact tcactttatg ctaatattat ccttaagaaa ggagtagttg
    14461 ttgaaagaga aaatgtttaa tagaggaatt ttagttgcca gtcatggtga ttttgctagc
    14521 ggagctctca tgaccgcaga aatgtttgtt ggtgagacaa caaatgatag agttaggaca
    14581 ttaggtttga tgcctggaga gaatattgta gagtttgagc attattttaa aaatcaagtg
    14641 gatgaactgt tagactcaaa tcaagaggtt atcgttttga ctgacttgat tggaggaagt
    14701 cctaataatg tggctttgtc acggttttta aatttggatt cagttgatat tgtaacaggg
    14761 tttaatattc ctctcctagt ggaattaata tcaaattatg attcaaaaat caatttagaa
    14821 gaaattgttc acaatgctca aaatagtttg tttaatgtta aacaacaact taacgtagag
    14881 gaggaagaaa atttatgtct atagagtttg ttcgtattga tgaccgtctg gtacatggtc
    14941 aagttgtcac tacgtggcta aaaaagtatg atattgagca agttatcatt gttaatgatc
    15001 gcatctcaga agataaaaca cgacaatcta ttttaaagat ttctgcacca gtaggtttaa
    15061 aaattgtttt ctttagtgta aaacggtttg tggaagtttt aaactctgtg ccaataaaaa
    15121 agagaacaat gctgatatat acaaatccaa aagatgtgta tgattctatt gaaggaaatt
    15181 taaaattgga gtacctcaat gtaggacaga tgagtaaaac ggaggaaaat gaaaaggtaa
    15241 cgggaggtgt agctctaggt gaagaagaca aatattattt taagaaaata gttgataagg
    15301 gaacgagagt tgaaattcaa atggttccta atgataaagt tacaatgttg gaaaaatttt
    15361 tataaaaata atttaaggag gtacagtata tgctattcac acaagcatta ctggtgacat
    15421 tagttgggat tattgccact attgactata atggaccgtt atttatgatt caccgtccgt
    15481 tagttacaag tgcaatggtt ggcttagtct taggagattt cacccaaggt gttcttattg
    15541 gttcagctct tgaattaact tggctcggtg taacaggtat tggaggttac actccaccag
    15601 atactatttc aggtgcgatt attggtactg catttggtat tttatctggt caaggagaaa
    15661 ctgctggtat cgctatagca gttccaattg cagttgctac ccaacagttg gatgttcttg
    15721 caaaaacttt agatgtttat tttgtgaaaa aagctgataa tgatgctaaa aacggagatt
    15781 attcaaagat cggtttttat cattattcaa gtttggtttt aatcacgtta tttaaaattg
    15841 taccaatttt cctagctatt atgcttggag gggaatatgt ggcagacttg tttgctaagg
    15901 ttccaccaat cgttatgcag ggacttaact ctgcaggtgc tttactacct tcaattggtt
    15961 ttggtatgct tttaaatatg atgctcaaga aaaatatgtg ggtattcttg ttgattggat
    16021 tcatttgttc tgtgtatgga ggaatgtcaa ccattgggat ctcactagtt ggtattgcgg
    16081 tagcatactt ctacgatatg attggaagta aaccacaaga aacaacttca agtagtgatg
    16141 ttgaggagga tcttgatcta tgatgaataa taaagtaact aaagttgaac ttaaaaaagt
    16201 tttcaaacga agttttatgt atggttcttc atggaactat gagagaatgc agaacctagg
    16261 ttttctatat acaattcttc cagtattgaa aaaactatac ccagacaaag attcagcttc
    16321 tcctgcaatg aaacgtcacc ttgagttttt caatactcat caaacagcgg caccatttat
    16381 tcttggagtt acttccgcta tggaagaaca agaaggaaat gaaggtgcag cttcaattac
    16441 tggtattaaa gttggcttga tggggccact ggctggtcta ggagatagtt tgttctggct
    16501 gacactagtt cctatctgtt ttagtattgg tgcgtcttat tctaaagacg gcggtgcttt
    16561 aggtatcttt atcgccttaa tattgtttaa tattattaat attcctgtta aatatttcgg
    16621 tttgaaatat gggtatacta agggttctag tcttatccaa gaaaataata caaaaggaac
    16681 attgaatcgc gttacgagta tggcgacagc attagggcta gtactagtgg gtggtttgat
    16741 tccatcaatg gttggtatta attttggatt agaatttaag cagggggaac ttgttatttc
    16801 tgttcaagaa atgattacaa aattaattcc aggatttatc cctatggctt tgactttatt
    16861 aatgtgtaaa ttaattagaa aaggaaagaa tccggttgta ctaatcttta gtgttatggc
    16921 tattggagtt atcctagttg ttttaggaat tttgaagtag tagaaagtgt ggaggtggta
    16981 tttgggatat cacctccatt ttggaagaga ggtaaagagt gaaattatgg tataagaaag
    17041 ctgcctcaaa ttggaatgaa gccttgccga ttgggaacgg tcatttaggt ggtatgattt
    17101 atggttcagc tacaaaagaa tgtattcaac taaacgatga gactatttgg tatagaggaa
    17161 agtcagatag aaataatcca gactcactat tgcatcttaa aaaaattcgg gaatatcttt
    17221 tagatggaga aattcagaaa gccgaagaat tgataaagtt aacaatgttt gctaccccaa
    17281 gagatcaaag ccactatgaa ttacttgggg aactttacat tgagcatata gatattcagt
    17341 cttgtgctct ttcattgtat gaaagagagc tagatttaga tacagctatt tctaatgttg
    17401 tgtttgagcc taatagttgt aatttacaaa taaaaagaga atattttacg agttttaata
    17461 agaatatttt atgttgccgt atagtgtcat cagttcaaaa cacattaaat ttaaacatta
    17521 atttgggtag aaataaacgg tttaatgacg aagtatctaa actggattca agtacaattt
    17581 taatgtcggc ctctgctgga ggtagaaaag gtgttcagtt taaagtagta tgtcattcta
    17641 aagttacgga tggtgaagta agtgtattgg gagagacaat agttattcgg aatgctacag
    17701 aggtatttct ttatctcaaa tcaatgacgg attattgggg aaatatagat atttcttctc
    17761 ttcagggaga atttagtagt attgattact ttacagaaaa agatgaacat gtaaaaaaat
    17821 atcaggagca atttaataga gttgatttta aactagacta tagtaaagat tgtcttagca
    17881 ttccaacgaa tctacttctt gaaaacacta aaaagtatag taactacttg actaacttgt
    17941 tatttcatta tggaagatat ctgttaatat cgtctagtca accgaatggt ttacctgtca
    18001 atcttcaagg aatatggtgt gatgaattaa atccaatttg gggttctaaa tatacgatta
    18061 atattaatac tcaaatgaat tattggatgg taggtccatg tgatttacca gaagtagaat
    18121 atccattatt tgatatgctc gaaagaatga gagaaccggg aagactaacc gctaagaaaa
    18181 tgtatggagc tagaggtttt acagcacatc ataatacgga tggttttggc gatacggctc
    18241 cccaatctca tgccatgggg gctgcaattt gggtattaac tattccatgg ttatgtactc
    18301 atatttggga acactattta tatttccaag atgagcgtat tcttacggaa cattttgaaa
    18361 tgataaaaga agcatttctt ttctttgaag attatttatt tgaggtggat ggctacttga
    18421 tgacaggtcc aagtgtctca ccggaaaata aatatcgctt aaaaaatggt attgaaggaa
    18481 atgcttgtct atcatctaca attgataatc aaattctaag atatttttgt gattcatgca
    18541 ttggcattgc aaaacaatta ggagacaatt cggattttat tagtcgtgtg aaggagttaa
    18601 aaaagaaact acctaaaaca aaaataggta gtaatgggca aatccaagaa tggttagaag
    18661 attatgaaga agtagagcct gggcatagac acatttcacc tctatttggg ctttatcctt
    18721 ataatgagat tgatattcat aaaactccgg aattagcaga agcagctaaa atcactatca
    18781 ataggagatt atcaaacgct aactttttat cttcacagga gagggagcaa gcgattaata
    18841 attggttagt aagtggtttg catgctagta cacaaacagg ttggagtgct gcatggctga
    18901 ttcatttttt tgcgagacta tatcaaggtg aacctgctta taaccagatt aatggtttgt
    18961 taaataatgc gactcttggc aatttatttc ttgaccatcc accatttcaa attgatggta
    19021 atttaggttt ggtgagtgga atttgtgaat tattagtaca gagccatcat aattggttat
    19081 cactaattcc agctttacct tctgcttggt cagaaggaga agtgaaaggt ttcagagtaa
    19141 gaggaggata taaggtatcg tttgcttgga aaaatgggga tataacattc ctaaaattgg
    19201 aaggaggaaa caaagatcaa aaagtaagag taagaatata tggcaaaaat actgatgtac
    19261 aaaatattga attggtattt aattcagaaa aaattattga gttaaatttt taggtataag
    19321 tcatgaataa agaaaaaata aaaagaaaat taatcacaat actgtttgta tgtattggga
    19381 tgttatgttt tggattgtta gcaggagtta aggctgataa tcgtgttcaa atgagaacga
    19441 cgattaataa tgaatcgcca ttgttgcttt ctccgttgta tggcaatgat aatggtaacg
    19501 gattatggtg ggggaacaca ttgaagggag catgggaagc tattcctgaa gatgtaaaac
    19561 catatgcagc gattgaactt catcctgcaa aagtctgtaa accaacaagt tgtattccac
    19621 gagatacgaa agaattgaga gaatggtatg tcaagatgtt ggaggaagct caaagtctaa
    19681 acattccagt tttcttggtt attatgtcgg ctggagagcg taatacagtt cctccagagt
    19741 ggttagatga acaattccaa aagtatagtg tgttaaaagg tgttttaaat attgagaatt
    19801 attggattta caataaccag ttagctccgc atagtgctaa atatttggaa gtttgtgcca
    19861 aatatggagc gcattttatc tggcatgatc atgaaaaatg gttctgggaa actattatga
    19921 atgatccgac attctttgaa gcgagtcaaa aatatcataa aaatttggtg ttggcaacta
    19981 aaaatacgcc aataagagat gatgcgggta cagattctat cgttagtgga ttttggttga
    20041 gtggcttatg tgataactgg ggctcatcaa cagatacatg gaaatggtgg gaaaaacatt
    20101 atacaaacac atttgaaact ggaagagcta gggatatgag atcctatgca tcggaaccag
    20161 aatcaatgat tgctatggaa atgatgaatg tatatactgg gggaggcaca gtttataatt
    20221 ttgaatgtgc cgcgtataca tttatgacaa atgatgtacc aactccagca tttactaaag
    20281 gtattattcc tttctttaga catgctatac aaaatccagc tccaagtaag gaagaagttg
    20341 taaatagaac aaaagctgta ttttggaatg gagaaggtag gattagttca ttaaacggat
    20401 tttatcaagg actttattcg aatgatgaaa caatgccttt atataataat gggagatatc
    20461 atattcttcc tgtaatacat gagaaaattg ataaggaaaa gatttcatct atattcccta
    20521 atgcaaaaat tttgactaaa aatagtgagg aattgtctag taaagtcaac tatttaaact
    20581 cgctttatcc aaaactttat gaaggagatg ggtatgctca gcgtgtaggt aattcctggt
    20641 atatttataa tagtaatgct aatatcaata aaaatcagca agtaatgttg cctatgtata
    20701 ctaataatac aaagtcgtta tcgttagatt tgacgccaca tacttacgct gttgttaaag
    20761 aaaatccaaa taatttacat
//
LOCUS       DAVBBS010000038        20725 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVBBS010000038 DAVBBS010000000
VERSION     DAVBBS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20725
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.038"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..916)
                     /gene="cysK"
                     /locus_tag="VQU32_001372"
     CDS             complement(<1..916)
                     /gene="cysK"
                     /locus_tag="VQU32_001372"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET2472813.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYE"
     gene            complement(1201..1836)
                     /locus_tag="VQU32_001373"
     CDS             complement(1201..1836)
                     /locus_tag="VQU32_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HET2472814.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVSVNLV"
     gene            1892..3190
                     /locus_tag="VQU32_001374"
     CDS             1892..3190
                     /locus_tag="VQU32_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2472815.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVIEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            3187..3849
                     /locus_tag="VQU32_001375"
     CDS             3187..3849
                     /locus_tag="VQU32_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET2472816.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQACQFWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            3929..4477
                     /gene="raiA"
                     /locus_tag="VQU32_001376"
     CDS             3929..4477
                     /gene="raiA"
                     /locus_tag="VQU32_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET2472817.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            4613..6586
                     /locus_tag="VQU32_001377"
     CDS             4613..6586
                     /locus_tag="VQU32_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET2472818.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDALGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNTVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            6583..7035
                     /gene="rplI"
                     /locus_tag="VQU32_001378"
     CDS             6583..7035
                     /gene="rplI"
                     /locus_tag="VQU32_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET2472819.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            7079..8431
                     /gene="dnaB"
                     /locus_tag="VQU32_001379"
     CDS             7079..8431
                     /gene="dnaB"
                     /locus_tag="VQU32_001379"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET2472820.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            8433..8699
                     /locus_tag="VQU32_001380"
     CDS             8433..8699
                     /locus_tag="VQU32_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472821.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            8788..10134
                     /gene="cbpD"
                     /locus_tag="VQU32_001381"
     CDS             8788..10134
                     /gene="cbpD"
                     /locus_tag="VQU32_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HET2472822.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSGTVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      10217..10319
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10390..10680
                     /locus_tag="VQU32_001382"
     CDS             10390..10680
                     /locus_tag="VQU32_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HET2472823.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            10643..11401
                     /locus_tag="VQU32_001383"
     CDS             10643..11401
                     /locus_tag="VQU32_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2472824.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            11439..12446
                     /locus_tag="VQU32_001384"
     CDS             11439..12446
                     /locus_tag="VQU32_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2472825.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            12446..13174
                     /locus_tag="VQU32_001385"
     CDS             12446..13174
                     /locus_tag="VQU32_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2472826.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            13293..13751
                     /locus_tag="VQU32_001386"
     CDS             13293..13751
                     /locus_tag="VQU32_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HET2472827.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            13753..16185
                     /locus_tag="VQU32_001387"
     CDS             13753..16185
                     /locus_tag="VQU32_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2472828.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFMELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALVLGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     ALRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKVKYVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            16827..17480
                     /gene="rr06"
                     /locus_tag="VQU32_001388"
     CDS             16827..17480
                     /gene="rr06"
                     /locus_tag="VQU32_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HET2472829.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            17477..18817
                     /gene="hk06"
                     /locus_tag="VQU32_001389"
     CDS             17477..18817
                     /gene="hk06"
                     /locus_tag="VQU32_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HET2472830.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILFLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            18900..19427
                     /locus_tag="VQU32_001390"
     CDS             18900..19427
                     /locus_tag="VQU32_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HET2472831.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            19506..>20725
                     /locus_tag="VQU32_001391"
     CDS             19506..>20725
                     /locus_tag="VQU32_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HET2472832.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEGVRSGD
                     TPKVTSSGDEVESHLKKILSEIQTQLDRKRHAKTVALINKLQDIKRTYSYKLNVLEEK
                     PKAELTSKTKEELTAAFEQFKKDTLPTEPGKKVAEAEKKVAEAEKKAEDQKEEDRRNY
                     PTNTYKTLELEIAEFDVKVKEAELELLKEKAKKPLNEGTIKQAKEKVESKKAEATRLE
                     NIKTDRKKAEEEAKRKAAEEDKVKEKPAEQPQPAPAPQPEKPAPKPEKPAPAPKPENP
                     APKPEKPAPAPKPENPAEQPKAEKPADQQAEEDYARRSEEEYNRLTQQQPPKTEKPAQ
                     PSTPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGWLQNNGSWYYL
                     NANGSMATGWLQNNG"
BASE COUNT         6510 a         3429 c         4696 g         6090 t
ORIGIN      
        1 attcataaag tgctgtagag agataacgtt caccgttatc tggtgctagg gcaaggactt
       61 ttttacctgt acctaatttt ttggcaacct cgatggctcc gtagatagct gcagctgagg
      121 aaatccctac aaggaagcct tcttttccac caatttcacg tccgagtgca agagcgtcat
      181 ctgatgttac acgaacgata ccatcatagg ctttagtatc aagtgtatca ggaataaatc
      241 cagctgagat accttgaatt ttgtgaggac caggtttttc accagataga atagcagatt
      301 catctgcttc tactgcaaaa acttgaatgt tagaattttc tgatttgagt gcatgagaaa
      361 caccagaaat cgttccacca gtacctactc cagcaacaaa ggcatctaat ccatctttac
      421 cgaaagcagc tagtatctca gctcctgttg ttctttcgtg tacttctgga ttagctggat
      481 tgtcaaattg aagaggaagg aaaccatcac gttcagcagc gatttcttga gccttagcaa
      541 tagcaccttt cattccctcg ctaccaggag ttaggacgag ttcagcacca taagcttgga
      601 taattttacg tcgttctaca ctcatagttt caggcataac gatgacgact ttataccctt
      661 tagcagcacc tacccatgaa agtccaatac cggtgtttcc acttgttgct tcaacaatag
      721 tagaaccaag tttcagaata ccatcttgtt cagctttttc aatcatgcta agggcaatac
      781 ggtcttttac agatgaacca ggattaaatg cttcaagctt tatatagacg tctgcagcac
      841 cttctggcac gatgttgtta agtttaacaa tcggtgtttg accgattaat tcagtaatgt
      901 tgttataaat agacatatga atacctcttt gtataagata tctttagtat aacgctatcc
      961 ataccatttg taaaatatag aaatcctatc gaagcgatag gatttctata tatcatagat
     1021 caaagtcatt aattttcaag tgattgcgtt cagttaattc tagtaaatag gtgtgaagga
     1081 aaacgatatc aggtttcgtt tgaattgctt cattttctag tatattttgg tacgcatctt
     1141 tttattttgc tcgctaaagt agttgtgaga atgtcaacga aacgctgtat tattcattgt
     1201 ttacactaag tttacagaaa cttcaacctc tcgtaaacct tggtcagtta aagtgacttt
     1261 tccattaaaa aactccacaa gtgcagcttt aatagtttct ttttcttctt tatcaacata
     1321 aatcatcgta tcgacttgat ctgtaaagtt tgtatccagc tccatgagat catgttcttt
     1381 aaggaagtta ctgtactctt ggtactgagc ataagacatt tgaatagcaa tgccagcctg
     1441 ttcttttatt tcaataatac caatttcttt gacagctaag gcgacactgc cggcgtaagc
     1501 acgaattagt cctccagcgc ctagtttaat accaccaaag tagcgtgtca cgaccacaca
     1561 gacattggtg agattgtgat tttctagtac cccaagcatg ggaacaccag cagtaccact
     1621 aggctcacca tcatcacttg tacgtttaat ttcactacgt tctccaataa taaaggcaga
     1681 gcagttatgt gtcgctttgt agtgttcttt tttgatggca gtaatgaagt cacgagcctc
     1741 ttcttcgcta taaacacgct tggcatggca gataaagcga gattttttga tttcttcttg
     1801 gacttggccg tcctctttaa ttgtcctaaa ttccatgatt taattgtact aaaaaataat
     1861 ctaaagtgct agtttttacg aataaagaag tatgaaagta aatttagatt atctcggtcg
     1921 tttatttact gagaatgaat taacagaaga agaacgtcag ttggcggaga aacttccagc
     1981 aatgagaaag gagaagggga aacttttctg tcaacgttgt aatagtacta ttctagaaga
     2041 atggtatttg cccatcggtg cttactattg tcgagagtgc ttgctgatga agcgagtcag
     2101 aagtgatcaa actttatact attttccgca ggaggatttt ccgaagcaag atgttctcaa
     2161 atggcgcggc caattaactc cttttcaaga gaaggtgtca gagggactgc ttcaagcagt
     2221 agacaagcaa aagccaacct tagttcatgc ggtaacagga gctggaaaga cagaaatgat
     2281 ttatcaagta gtggctaaag tgatcaatgc gggtggtgca gtgtgtttgg ctagtcctcg
     2341 catagatgtt tgtttggagc tgtacaagcg cctgcaacag gatttttctt gcggtatagc
     2401 tttgctacat ggagaatcgg aaccttattt tcgaacacca ctagttgttg caacaaccca
     2461 tcagttattg aagttttatc aagcttttga tttgctgata gtggatgaag tagatgcttt
     2521 tccttatgtt gataatccca cgctttacca cgctgtcaag aatagtgtaa aggagaatgg
     2581 attgagaatc tttttaacag cgacttcgac caatgagtta gataaaaagg tccgtttagg
     2641 agaactaaaa agactgagtt taccgagacg gtttcatgga aatccgttga ttattccaaa
     2701 accaatttgg ttatcggatt ttaatcgcta cttagacaag aatcgtttgt caccaaagtt
     2761 aaagtcctat attgagaagc agagaaagac agcttatccg ttactcattt ttgcttcaga
     2821 aattaagaaa ggggagcagt tagaagaaat cttacaggag caatttccaa atgagaaaat
     2881 tggctttgta tcttctgtaa cagaggatcg attagagcaa gtacaagctt ttcgagatgg
     2941 agaactgaca atacttatca gtacgacaat cttggagcgt ggagttacct tcccttgtgt
     3001 ggatgttttc gtaatagagg ccaatcatcg tttgtttacc aagtctagtt tgattcagat
     3061 tggtggacga gttggacgaa gcatggatag accgacagga gatttgcttt tcttccatga
     3121 tgggttaaat gcttcaatca agaaggcgat taaggaaatt cagatgatga ataaggaggc
     3181 tggtctatga agtgcttgtt atgtgggcag actatgaaga ctgttttaac ttttagtagt
     3241 ctcttacttc tgaggaatga tgactcttgt ctttgttcag actgtgattc tacttttgaa
     3301 agaattgggg aagagaactg tccaaattgt atgaaaacag agttgtcaac aaagtgtcaa
     3361 gcttgtcaat tttggtgtaa agaaggagtt gaagtcagtc atagagcgat ttttacttac
     3421 aatcaagcta tgaaggattt tttcagtcgg tataagtttg atggagactt cctgttaaga
     3481 aaagttttcg cttcattttt aagtgaggag ttgaaaaagt acaaagagta tcaatttgtt
     3541 gtaattcccc taagtcctga tagatatgct aacagaggat ttaatcaggt tgagggcttg
     3601 gtagaggcag caggctttga gtatctagat ttattagaga aaagagaaga gagagccagt
     3661 tcttctaaaa atcgttcaga gcgcttgggg acagaacttc ctttctttat taaaagtgga
     3721 gtcactattc ctaaaaaaat cctacttata gatgatatct atactacagg agcaactata
     3781 aatcgtgtta agaaactgtt ggaagaagct ggtgctaagg atgtaaaaac attttccctt
     3841 gtaagatgag gaaaaaattt gcaaaaatag aatgaaagcg ttataataaa atcataaaaa
     3901 caaaaatgtt tagaaagaag gtactcatat gattaaatat agtatccgtg gtgaaaacct
     3961 agaagtaaca gaagcaattc gtgattatgt agtttctaaa ctcgaaaaga tcgaaaagta
     4021 cttccaacca gaacaagagt tggatgcccg aattaactta aaagtttatc gtgaaaaaac
     4081 ggctaaagtg gaagtaacga ttccgcttgg atctattact ctccgcgcag aagatgtatc
     4141 tcaagatatg tatggttcaa ttgaccttgt aactgataaa attgaacgtc agattcgtaa
     4201 aaataaaaca aaaatcgagc gtaaaaataa aaataaggta gcaactggtc aattatttac
     4261 agatgctttg gtggaagatt caaatattgt ccagtctaaa gttgttcgtt caaaacaaat
     4321 tgatttaaaa ccaatggatt tggaagaagc aattctacaa atggatttat tggggcatga
     4381 tttctttatc tatgtggatg ttgaagatca gacaaccaat gtgatttatc gtcgtgagga
     4441 tggcgaaatt ggtttgttag aggttaaaga atcttaattt caatatgtgt aaaggtaggt
     4501 ttactgaatt gtaaactacc tttttcttat aattgataga attactgatt acacttttaa
     4561 aaagtcgctt tttgaggggt attatggtat aatgggtgcc aagaggtttt gaatgaaaaa
     4621 attttatgta agtccaattt ttcctattct agtaggattg attgcgtttg gagtcttatc
     4681 cactttcatt atttttgtta ataataatct gttgacggtt ttaattttgt ttctttttgt
     4741 aggaggctat gtttttttat ttaagaaact gagagtgcat tatacaagga gtgatgtaga
     4801 acagatacag tatgtaaacc accaagcgga agaaagtttg acagctctat tggaacagat
     4861 gcctgtaggt gttatgaaat tgaatttatc ttctggagag gttgagtggt ttaatcccta
     4921 tgctgaattg attttgacca aggaagatgg tgattttgat ttagaagctg ttcaaacgat
     4981 tatcaaggct tcagtaggaa atccgtctac ttatgccaag cttggtgaga agcgttatgc
     5041 tgttcatatg gatgcttctt ccggtgtttt gtattttgta gatgtatcca gggaacaagc
     5101 cataacagat gaattggtaa caagtcgacc agtgattggg attgtctctg tggataatta
     5161 tgatgatttg gaggatgcaa cttctgagtc agatattagt caaatcaata gttttgtagc
     5221 taattttata tcagagtttt cagaaaaata catgatgttt tctcgtcggg taagtatgga
     5281 tcgattttat ctatttactg actacacggt gcttgagggc ttgatgaatg ataaattttc
     5341 tgttattgat gctttcagag aagagtcgaa acagagacag ttgcccttga ccttaagtat
     5401 gggattttct tatggcgatg gaaatcatga tgagataggg aaagttgctt tgctcaattt
     5461 gaacttggct gaagtacgtg gtggcgacca ggtggttgtt aaggaaaacg acgaaacgaa
     5521 aaatccagtt tattttggtg gtgggtctgc tgcttcaatc aagcgtacac ggactcgtac
     5581 gcgcgctatg atgacagcta tttcagataa gattcggagt gtagatcagg tttttgtagt
     5641 cggtcacaaa aatttagaca tggatgcttt gggctctgct gtaggtatgc agttgttcgc
     5701 cagcaatgtg attgaaaata gctatgctct ttatgatgaa gaacaaatgt ctccagatat
     5761 tgaacgagct gtttcattca tagaaaaaga aggagttacg aagttgttgt ctgttaagga
     5821 tgcattgggg atggtgacca atcgttcttt gttgattctt gtagaccatt caaagacagc
     5881 cttaacatta tcaaaagaat tttatgattt atttacccaa accattgtta ttgaccacca
     5941 tagaagggat caggattttc cagataatac ggtcattact tatatcgaaa gtggtgcaag
     6001 tagtgccagt gagttggtaa cggaattgat tcagttccag aattctaaga aaaatcgttt
     6061 gagtcgtatg caagcaagtg tcttgatggc tggtatgatg ttggatacta aaaatttcac
     6121 ctcgcgagta actagtcgga catttgatgt tgctagctat ctcagaacgc gcggaagtga
     6181 tagtattgct atccaggaaa tcgctgcgac agattttgaa gaatatcgtg aggtcaatga
     6241 acttatttta caggggcgta aattaggttc agatgtacta atagcagagg ctaaggacac
     6301 gaaatgctat gatacagttg ttattagtaa ggcagcagat gccatgttag ccatgtcagg
     6361 tattgaagcg agttttgttc ttgcgaagaa tacacaagga tttatctcta tctcagctcg
     6421 aagtcgtagt aaactgaatg tacaacggat tatggaagag ttaggcggtg gaggccactt
     6481 taatttggca gcagctcaaa ttaaagatgt aaccttgtca gaagcaggtg aaaaactgac
     6541 agaaattgta ttaaatgaaa taaaggaaaa ggagaaagaa gaatgaaagt aatcttttta
     6601 gcagatgtta aaggaaaagg taaaaaaggc gaaattaagg aagtaccaac agggtatgcg
     6661 caaaactttc ttatcaaaaa gaatctagcc aaagaagcga ctgctcaagc tgtaggtgaa
     6721 cttcgtggta aacaaaaatc ggaagaaaaa gctcacgctg agatgattgc agaaggaaaa
     6781 gcaattaaag cacaacttga agcagaagaa actgttgtag aatttgttga aaaagttggt
     6841 ccagatggtc gtacctttgg ctctattacc aataagaaga ttgcagaaga attgcaaaaa
     6901 caatttggaa ttaagattga taaacgtcat attcaagtac aagctccgat tcgagcggtt
     6961 ggtttgattg atgtgccagt gaaaatctat caagatatca caagtgtaat caatcttcgt
     7021 gtgaaagaag gataagttta caccttcttg acaagattgt aaaaggaagg gaagtctgat
     7081 ggcagaagta gaagagttac gagtacaacc tcaagatatc ttagctgagc aatccgtttt
     7141 aggggctatc tttattgatg agagtaaact tgtttttgtg cgagaataca ttgagtctcg
     7201 ggactttttt aagtatgccc atcgtttgat tttccaagcc atggtcgatt tatccgatcg
     7261 tggtgatgcc atagatgcaa caacggttcg tactatcctt gataatcaag gtgatttaca
     7321 gaatattggt ggcttgtctt acttggttga gattgttaat tctgtgccaa cttctgctaa
     7381 tgcggagtat tatgctaaga ttgttgcaga aaaagcaatg ctacgtcgtt taattgccaa
     7441 gttgacagag tctgtcaacc aagcttacga agcgtcacaa ccagctgatg aaattattgc
     7501 tcaggcagaa aaagggctga ttgatgtcag tgaaaatgca aatcgaagcg ggtttaagaa
     7561 cattcgagat gtgttgaatc tcaactttgg aaatctggaa gctcgctcgc aacaaacgac
     7621 cgatattaca ggtattgcga caggttatcg tgatttggat catatgacaa caggacttca
     7681 tgaggaggag ttgattatct tagcagctcg tccagcagtt ggtaagacag catttgcctt
     7741 gaatatcgct cagaacattg ggactaagtt ggacaaaacg gttgctattt tttcactcga
     7801 aatgggtgcg gaaagcttgg tagatcgtat gttagctgca gaaggcttag tggagtcaca
     7861 ttctatccgt acagggcaat tgacagatga ggagtggcaa aaatatacta ttgctcaggg
     7921 taatctagct aacgccagta tctatatcga tgatacgcca ggtattcgga ttacagagat
     7981 tcgttctcgt tctcgtaaat tggctcaaga aactggaaat cttggtttga ttttgataga
     8041 ctatttgcaa cttatcacgg gaactggtcg agaaaatcgt caacaagaag tttctgaaat
     8101 ttctcgtcag ttgaaaatac tagccaagga attgaaggtt ccagtaatcg ctctgagtca
     8161 gctttctcgt ggtgtagaac aacgtcagga caagagaccg gtcttgtctg atattcgtga
     8221 atctgggtct attgagcagg acgctgatat cgtagctttt ctctatcgcg atgactacta
     8281 tgaacgtggt ggtgaagaag aggagggtat cccaaataat aaggtggaag ttattatcga
     8341 gaaaaaccgt agtggagctc gtggaacagt ggaattgatt ttccaaaaag aatacaataa
     8401 attttcaagt atctcaaaga gggaggcata agatgtcaga tgcatttaca gatgtagcca
     8461 agatgaaaaa aatcaaagaa gaaatcaagg cacatgaggg acaagtcgta gaaatgactt
     8521 tggagaatgg tcgtaagcgc caaaaaaata gattgggtaa gctaattgaa gtttatccat
     8581 ctctatttat tgtggagttt ggggatgtgg aaggagataa acaagttaat gtttacgttg
     8641 aatcctttac ttactcagat attcttacag aaaagaattt gattcattat cttgactaaa
     8701 gtgagaaatt ttctcacttt ttcttttttc tccgaataat ttaggtgaag gcaatcatcg
     8761 ctttatatta tttttcaagg aggaagaatg aaaattttac cgtttatagc aagaggaaca
     8821 agttattact tgaagatgtc agttaaaaag cttgttcctt ttttagtagt aggattgatg
     8881 ctagcagctg gtgatagtgt ctatgcctat tccagaggaa atggatcgat tgcgcgtggg
     8941 gatgattatc ctgcttatta taaaaatggg agccaggaga ttgatcagtg gcgcatgtat
     9001 tctcgtcagt gtacttcttt tgtagccttt cgtttgagta atgtcaatgg ttttgaaatt
     9061 ccggcagctt atggaaatgc gaatgaatgg ggacatcgtg ctcgtcggga aggttatcgt
     9121 gtagataata caccgacgat tggttccatt acttggtcta ctgcaggaac ttatggtcat
     9181 gttgcctggg tgtcaaatgt aatgggagat cagattgaga ttgaggaata taactatggt
     9241 tatacagaat cctataataa acgagttata aaagcaaaca cgatgacagg atttattcat
     9301 tttaaagatt tggatggtgg cagtgttggg aatagtcaat cctcaacttc aacaggcgga
     9361 actcattatt ttaagaccaa gtctgctatt aaaactgaac ccctagttag tgcaactgtg
     9421 attgattact attatcctgg agagaaggtt cattatgatc aaattctcga aaaagacggc
     9481 tacaagtggt tgagttatac tgcctataat ggaagctatc gttatgttca attggaggct
     9541 gtgaataaaa atcctctagg taattctgtt ctttcttcaa caggtggaac tcattatttt
     9601 aagaccaagt ctgctatcaa aactgaaccc ctagttagcg gaactgtgat tgattactat
     9661 tatcctgggg agaaggttca ttatgatcag atacttgaaa aagacggcta caagtggttg
     9721 agttatactg cctataatgg aagtcgtcgc tatatacagc tagagggagt gacttcttca
     9781 caaaattatc agaatcaatc aggaaatatc tctagctatg gatccaataa tagttcaact
     9841 gtcggttgga agaaaataaa tggtagttgg tatcatttca aatcaaatgg ttctaaatca
     9901 acaggatggc tgaaagacgg ttctagctgg tattatttga aattatctgg tgaaatgcag
     9961 acaggatggt taaaggagaa tggctcgtgg tattatctgg gtagttcagg ggcaatgaaa
    10021 acaggctggt accaggtctc tggtaagtgg tattattctt actcttcagg cgccttagct
    10081 gttaatacga cggtggatgg ctacagagta aacagtgatg gagaacgagt atagaaaatt
    10141 ggagtaggag aaatttcctg ctcttttctg taagcagttt ccttgacatt tttcttattt
    10201 tgcgctatca tatatacata taatatatgg gagtctgtgt acagtctgag aggaagtgtt
    10261 aaacttcgac cgcacctgat ctgggtaatg ccagcggagg gaacgatact tagtctaatt
    10321 ttgcaccttt tccatgtatg gtaaaggttt ttcttttttt taaaaggaaa acgagaagag
    10381 gaggttctta tgaaagcaag cattgccttg caagttttac ccctagcaca ggggattgat
    10441 cggatagctg ttattgatca ggtcattgct tatctgcaaa ctcaagaagt gacgatggta
    10501 gtgacaccat ttgaaacggt cttggaaggg gagtttgatg agcttatgcg cattctaaaa
    10561 gaagcgctgg aagtggcagg gcaggaggca gacaatgtct ttgccaatgt caaaataaat
    10621 gtaggagaga ttttaagtat tgatgagaaa cttgaaaagt atactgagac gacacattag
    10681 tctattgggc tttctcggag tattgtcaat ctggcagtta gcaggttttc ttaaacttct
    10741 ccccaagttt atcctgccga cacctcttga aattctccag ccctttgttc gtgacagaga
    10801 atttctctgg caccatagct gggcgacctt gagagtggct ttactggggc tgattttggg
    10861 agttttgatt gcctgtctta tggctgtgct catggatagt ttgacttggc tcaatgacct
    10921 gatttaccct atgatggtgg tcattcagac cattccgacc attgccatag ctcctatcct
    10981 ggtcttgtgg ctgggttatg ggattttgcc caagattgtc ttgattatct taacaacaac
    11041 ctttcccatc atcgttagta ttttggacgg ttttaggcat tgtgacaagg atatgctgac
    11101 tttgtttagt ctgatgcgag caaacccttg gcaaatcctg tggcatttta aaatcccggt
    11161 tagcctgcct tacttttatg caggtctgag ggtcagtgtc tcctacgcct ttatcacaac
    11221 tgtggtatct gagtggttgg gaggttttga aggtcttggt gtttatatga ttcagtctaa
    11281 aaaactgttt cagtatgata ccatgtttgc cattattatt ctggtgtcga ttatcagtct
    11341 tttgggtatg aagctggtcg atatcagtga aaaatatgtg attaaatgga aacgtactta
    11401 aaatagagcg tttttgaaaa agaaaagagg aaatcaaaat gaagaaaaca tggaaagtgt
    11461 ttttaacgct tgtaacagct cttgtagctg ttgtgcttgt ggcctgtggt caaggaactg
    11521 cttctaaaga caacaaagag gcagaactta agaaggttga ctttatccta gactggacac
    11581 caaataccaa ccacacaggg ctttatgttg ccaaggaaaa aggttatttc aaagaagctg
    11641 gagtggatgt tgatttgaaa ttgccaccag aagaaagttc ttctgacttg gttatcaacg
    11701 gaaaggcacc atttgcagtg tatttccaag actacatggc taagaaattg gaaaaaggag
    11761 caggaatcac tgccgttgca gctattgttg aacacaatac atcaggaatc atctctcgta
    11821 aatctgataa tgtaagcagt ccaaaagact tggttggtaa gaaatatggg acatggaatg
    11881 acccaactga acttgctatg ttgaaaacct tggtagaatc tcaaggtgga gactttgaga
    11941 aggttgaaaa agtaccaaat aacgactcaa actcaatcac accgattgcc aatggcgtct
    12001 ttgatactgc ttggatttac tacggttggg atggtatcct tgctaaatct caaggtgtag
    12061 atgctaactt catgtacttg aaagactatg tcaaggagtt tgactactat tcaccagtta
    12121 tcatcgcaaa caacgactat ctgaaagata acaaagaaga agctcgcaaa gtcatccaag
    12181 ccatcaaaaa aggctaccaa tatgccatgg aacatccaga agaagctgca gatattctca
    12241 tcaagaatgc acctgaactc aaggaaaaac gtgactttgt catcgaatct caaaaatact
    12301 tgtcaaaaga atacgcaagc gacaaggaaa aatggggtca atttgacgca gctcgctgga
    12361 atgctttcta taaatgggat aaagaaaatg gtatccttaa agaagacttg acagacaaag
    12421 gcttcaccaa cgaatttgtg aaataatgac agaaattaga ctagagcatg tcagttatgc
    12481 ctatggtcag gagaggattt tagaggatat caacctacag gtgacttcag gcgaagtggt
    12541 ttccattcta ggcccaagtg gtgttggaaa gaccaccctc tttaatctaa tcgctgggat
    12601 tttagaagtt cagtcaggga gaattgtcct tgatggtgaa gaaaatccca aggggcgcgt
    12661 gagttatatg ttgcaaaagg atctgctctt ggagcacaag acggtgcttg gaaatatcat
    12721 tctgcccctc ttgattcaaa aggtggataa ggcagaagct atttcccgag cggataaaat
    12781 tcttgcgacc ttccagctga cagctgtaag agacaagtat cctcatgaac ttagcggtgg
    12841 gatgcgccag cgtgtagcct tactccggac ctaccttttt gggcacaagc tctttctctt
    12901 agatgaggcc tttagcgcct tggatgagat gacaaagatg gaactccacg cttggtatct
    12961 tgagattcac aaacagttgc agctaacaac cctgatcatc acgcatagta ttgaggaggc
    13021 cctcaatctc agcgaccgta tctatatctt gaaaaatcgc cctgggcaga ttgtttcaga
    13081 aattaaacta gattggtctg aagatgagga caaggaagtc caaaagattg cctacaaacg
    13141 tcaaatcttg gcagaattag gcttagataa gtagaaaaat agggagttgg tgaagattat
    13201 cctttaccag cgcccttttt cttttaaaaa tgagaaaatt tcggtataat agtcaaacaa
    13261 ggtcaaggtt taaagagaga ggtgggtttg ttatgagatt taaaaataca tcggatcata
    13321 ttgaggccta catcaaggcg attttagatc aatctggtat cgtggagttg caacggagtc
    13381 agttggcaga tacctttcag gttgttccta gtcagattaa ctacgtgatc aagacacgct
    13441 ttacggaaag tagaggctac ttggttgaaa gtaagcgtgg tggcggaggc tacattcgta
    13501 taggacggat tgagttttct agtcatcatg aaatgctccg ggagctgctt tactcgattg
    13561 gtgagcgagt cagtcaagaa atttatgagg atattctcca gcttttggtt gagcaggaat
    13621 tgatgaccaa gcaggagatg aatttgctag aatcagtagc tttggatcgc gttttaggag
    13681 aagaagctcc agttgttcga gcaaacatgc tacgtcaggt catacaagag gtagatagaa
    13741 aagggaagta agatgaacta ttcaaaagca ttgaatgaat gtatcgaaag tgcctacatg
    13801 gttgctggac attttggagc tcgttatcta gagtcgtggc acttgttgat tgccatgtct
    13861 aatcacagtt atagtgtagc aggggcaact ttaaatgatt atccgtatga gatggaccgt
    13921 ttagaagagg tggctttgga actgactgaa acggactata gccaggatga aacctttatg
    13981 gaattgccgt tctcccgtcg tttgcaggtt ctttttgatg aagcagagta tgtagcgtca
    14041 gtggtccatg ctaaggtact agggacagag cacgtcctct atgcgatttt gcatgatggc
    14101 aatgccttgg cgactcgtat cttggagagg gctggttttt cttatgaaga caagaaagat
    14161 caggtcaaga ttgctgctct tcgtcgaaat ttagaagaac gggcaggctg gactcgtgaa
    14221 gatctcaagg ctttacgcca acgccatcgt actgtggctg acaaacaaaa ttctatggcc
    14281 aatatgatgg gcatgccgca gactcctagt ggtggtctcg aggactatac gcatgatttg
    14341 acagagcaag cgcgttctgg caagttagaa ccagtcatcg gtcgggacaa ggaaatctca
    14401 cgtatgattc aaatcttgag ccgtaagact aagaacaacc ctgtcttggt tggggatgct
    14461 ggtgtcggga aaacagctct ggtgcttggt cttgctcagc gtattgccag tggtgatgtg
    14521 cctgcggaaa tggctaagat gcgcgtgtta gaacttgatt tgatgaatgt cgttgcaggg
    14581 acacgcttcc gtggtgactt tgaagaacgc atgaataata tcatcaagga tattgaagaa
    14641 gatggccaag tcatcctctt tatcgatgaa ctccacacca tcatgggttc tggtagcggg
    14701 attgattcga ctctggatgc ggccaatatc ttgaaaccag ccttggcgcg tggagctttg
    14761 agaacggttg gtgccaccac tcaggaagaa tatcaaaaac atatcgaaaa agatgcggct
    14821 ctttctcgtc gtttcgctaa agtgacgatt gaagaaccaa gtgtggcaga tagtatgact
    14881 attttacaag gtttgaaggc gacttatgag aaacatcacc gtgtacaaat cacagatgaa
    14941 gcggttgaaa cagcggttaa gatggctcat cgttatttaa ccagtcgtca cttgccagac
    15001 tctgctatcg acctcttgga cgaagcagca gcaacagtgc aaaataaggt aaagtatgta
    15061 aaagcagacg attcagattt gagtccagct gataaggccc tgatggatgg caagtggaaa
    15121 caggcagccc agctaatcgc aaaagaagag gaagtacctg tctacaaaga cttggtgaca
    15181 gagtctgata ttttgaccac cttgagtcgc ttgtcaggaa tcccagttca aaaactgact
    15241 caaacggatg ctaagaagta tctaaatctt gaagcagaac tccataaacg tgtcatcggt
    15301 caagatcaag ctgtttcaag cattagtcgt gcgattcgcc gtaatcagtc aggaattcgc
    15361 agtcacaagc gtccgatcgg ttcctttatg ttcctaggac ccacaggtgt cggtaagacc
    15421 gagttggcca aggctttggc agaagttctc tttgatgacg aatcagccct tatccgcttt
    15481 gatatgagtg agtatatgga gaaattcgca gccagccgtc taaatggagc tcctccaggc
    15541 tatgtgggtt acgaagaagg tggagagttg acagagaagg ttcgcaataa accctattcc
    15601 gttctcctct ttgatgaggt agagaaggcc cacccagata tttttaatgt tctcttgcag
    15661 gttctggatg acggtgtttt gacagatagc aagggacgca aggtcgattt ttcaaatacc
    15721 attatcatta tgacatcgaa cctaggtgcg actgcccttc gtgatgataa gactgttggt
    15781 tttggggcta aggatattcg ttttgaccag gaaaatatgg aaaaacgcat gtttgaagaa
    15841 ctgaaaaaag cttatagacc ggaattcatc aaccgtattg atgagaaggt ggtcttccat
    15901 agcctatcta gtgaccatat gcaggaagtg gtgaagatta tggtcaagcc tttagtggca
    15961 agtttggctg aaaaaggcat tgacttgaaa ttacaagctt cagctctgaa attgttagca
    16021 aatcaaggat atgacccaga gatgggagct cgcccacttc gcagaaccct gcaaacagaa
    16081 gtggaggaca agttggcaga acttcttctt aagggagatt tagtggcagg cagcacactt
    16141 aagattggtg tcaaagcagg ccagttaaaa tttgatattg cataaaagaa taaaagtatc
    16201 agcatctgac cataagtcac agtggagtga aactcaatga aaatcaaaga gcaaactagg
    16261 cagctagccg caggttgctc aaaacactgg tttgaggttg cagatagagc tgacgtggtt
    16321 tgaagagatt ttcgaagagt atgaaactaa aacctatagc ttctaaacga tccgtggttt
    16381 tcaccattca acacaaaatt catatgttta ttaccctccg tcgtatttgt cttagagcgt
    16441 gtgtagtaga aaaagagcag tcttatctga aatttttatt ctttcaaaag agacctgttt
    16501 cttttttgca tgtcaaatcc gttctagctg gtatttgaaa aatcaaacta atattcaatg
    16561 aaaatcaaag aacaaactag gcagctagcc acaggttgct caaaacactg ttttgaggtt
    16621 gtagatagag ctgacgtagt ttgaagagat tttcgaagag tataagctgc aagatgaatg
    16681 attttcttgt attgacgttg ttgttgacaa aaagtagcgg ataaatgaaa tccattccat
    16741 tatcatagat gataggctgg taggaaattt tcaaatagca tacaggaaat agatgtatgg
    16801 agttctggta gtagaaaggg agagagatga acattttagt tgcagatgac gaggaaatga
    16861 ttagagaagg aattgcagca tttctgacag aagagggtta tcatgtcatt atggctaagg
    16921 atggacaaga ggtcttggaa aaatttcaag atctccctat ccatctcatg gtattggatt
    16981 taatgatgcc taggaagagt ggttttgaag tgttaaaaga aatcaatcaa aagcacgata
    17041 ttcctgtcat cgtcttgagt gctctgggag atgaaactac tcagtcacag gtatttgatc
    17101 tctatgctga tgatcatgtg acaaaacctt tttctttggt actgcttgtc aagcgtatta
    17161 aggcgcttat cagacgttac tacgtcatag aggatatttg gcgatatcag gatgtaacag
    17221 tggattttac ctcttacaaa gcacattata aaaatgaaga aattgatctc aaaccaaagg
    17281 aattactggt actaaagtgt ttgattcagc ataaaaatca agttttaagt agagagcaga
    17341 tattggaaga aatttcaaaa gatgtagctg atttaccttg tgatagggtc gttgatgtct
    17401 atattcgtac tcttcgcaaa aaattagctt tagattgtat cgtgactgtg aaaaatgttg
    17461 ggtataagat tagcttatga taaaaaatcc taaattatta accaagtctt ttttaagaag
    17521 ttttgcaatt ctaggtggtg ttggtctagt cattcatata gctatttatt tgacctttcc
    17581 tttttattat attcaactgg agggggaaaa gtttaatgag agcgcaagag tgtttacgga
    17641 gtatttaaag actaagacat ctgatgaaat tccaagctta ctccagtctt attcaaagtc
    17701 cttgaccata tctgctcacc ttaaaagaga tattgtagat aagcgactcc ctcttgtgca
    17761 tgacttggat attaaagatg gaaagctatc aaattatatc gtgatgttag atatgtctgt
    17821 tagtacagca gatggtaaac aggtaacagt gcaatttgtt cacggggtgg atgtctacaa
    17881 agaagcaaag aatattttgt ttttgtatct tccatataca tttttggtta caattgcttt
    17941 ttcctttgtt ttttcttatt tttatactaa acgcttgctc aatcctcttt tttacatttc
    18001 agaagtgact agtaaaatgc aagatttgga tgacaatatt cgttttgatg aaagtaggaa
    18061 agatgaagtt ggtgaagttg gagaagttgg aaaacagatt aatggtatgt atgagcactt
    18121 gttgaaggtt attcatgagt tggaaagtcg taatgagcaa attgtaaaat tgcaaaatca
    18181 aaaggtttcc tttgtccgtg gagcatcaca tgagttgaaa acccctttag ccagtcttag
    18241 aattatccta gagaatatgc agcataatat tggagattac aaagatcatc caaaatatat
    18301 tgcaaagagt ataaataaga ttgaccagat gagccactta ttggaagaag tactggagtc
    18361 ttctaaattc caagagtgga cagagtgtcg tgagaccttg actgttaagc cagttttagt
    18421 agatatttta tcacgttatc aagaattagc tcattcaata ggtgttacaa ttgaaaatca
    18481 attgacagat gctaccaggg tcgtcatgag tcttagggca ttggataagg ttttgacaaa
    18541 cctgattagt aatgcaatta aatattcaga taaaaatggg cgcgtaatca tatccgagca
    18601 agatggctat ctctctatca aaaatacatg tgcgcctcta agtgaccaag aactagaaca
    18661 tttatttgat atattctatc attctcaaat cgtgacagat aaggatgaaa gttccggttt
    18721 gggtctttac attgtgaata atattttaga aagctatcaa atggactata gttttctccc
    18781 ttatgaacac ggtatggaat ttaagattag cttgtagaca gattagtttt ttattaaagt
    18841 tcatataggg ttaacataag tgtgttattc tttgtgtaga taaaagaaag gatactaata
    18901 tggtattagc gattatttta gtaacattct ttattcgatt gattttttta aagcgttcga
    18961 tagagaatga gaaacgaatc cttagcaatg gcgggaaaga atttggagtt gagaatacaa
    19021 aacgattaac tatggctcat attgtttttt atctctcttg cttggttgag gcaatggtgc
    19081 acaagacaat ttttgatggc atgggcatgg ttggtttagt cttgcttatt ttttctatgc
    19141 tgatgttaat gttggtgatt cacttgttgg gagatatttg gacagtgaag cttatgcttg
    19201 tcaataatca caaatatgta gatcatatct tgtttaggac agtaaaacat cctaattact
    19261 ttttaaatat tcttcctgag ttgattggct tgaccttgtt gagtcatgct tatgtgactt
    19321 ttgttttagt ttttccagtt tatgcagtta ttttgtatcg acgaatagct gaagaggaaa
    19381 agctattaca tgaagttata atcccaaatg gaagcataaa gagataaata caaaattcga
    19441 tttatataca gttcatattg aagtgatata gtaaggttaa agaaaaaata tagaaggaaa
    19501 taaacatgtt tgcatcaaaa agcgaaagaa aagtacatta ttcaattcgt aaatttagta
    19561 ttggagtagc tagtgtagta gttgctagct tgttcttagg aggagtagtt catgcagaag
    19621 gggttagaag tggggatacc cccaaggtta catctagtgg ggatgaagtc gagtcgcatc
    19681 taaaaaaaat attgagtgag atccaaacac aattagatag aaaacgacat gccaaaactg
    19741 tagcgctaat taacaagttg caagacatta agagaacgta ttcgtataaa ttaaatgttt
    19801 tagaagagaa gccgaaagct gagttgacgt caaaaacaaa agaagagtta accgcagctt
    19861 ttgagcaatt taaaaaagat acattaccaa cagaaccagg aaaaaaggta gcagaagctg
    19921 agaagaaggt tgcagaagct gagaaaaaag ccgaggatca aaaagaagaa gatcgtcgta
    19981 actacccaac caatacttac aaaacgcttg aacttgaaat tgctgagttc gatgtgaaag
    20041 ttaaagaagc ggagcttgaa ctattaaaag agaaagctaa gaaaccttta aacgagggca
    20101 caattaagca agcaaaagag aaagttgaga gtaaaaaagc tgaggctaca aggttagaaa
    20161 acatcaagac agatcgtaaa aaagcagaag aagaagctaa acgaaaagca gcagaagaag
    20221 ataaagttaa agaaaaacca gctgaacaac cacaaccagc gccggctcct caaccagaaa
    20281 aaccagctcc aaaaccagaa aaaccagctc cagctccaaa accagagaat ccagctccaa
    20341 aaccagaaaa accagctcca gctccaaaac cagagaatcc agctgaacaa ccaaaagcag
    20401 aaaaaccagc tgatcaacaa gctgaagaag actatgctcg tagatcagaa gaagaatata
    20461 atcgcttgac tcaacagcaa ccgccaaaaa ctgaaaaacc agcacaacca tctactccaa
    20521 aaacaggctg gaaacaagaa aacggtatgt ggtacttcta caatactgat ggttcaatgg
    20581 cgacaggatg gctccaaaac aatggctcat ggtactacct caacagcaat ggcgctatgg
    20641 cgacaggatg gctccaaaac aatggttcat ggtactatct aaacgctaat ggttcaatgg
    20701 caacaggatg gctccaaaac aatgg
//
LOCUS       DAVBBS010000039        20625 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVBBS010000039 DAVBBS010000000
VERSION     DAVBBS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20625
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.039"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..140)
                     /locus_tag="VQU32_001392"
     CDS             complement(<1..140)
                     /locus_tag="VQU32_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2472833.1"
                     /translation="MLYVGIDIAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LR"
     gene            395..1240
                     /locus_tag="VQU32_001393"
     CDS             395..1240
                     /locus_tag="VQU32_001393"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET2472834.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            complement(1387..2448)
                     /gene="dinB"
                     /locus_tag="VQU32_001394"
     CDS             complement(1387..2448)
                     /gene="dinB"
                     /locus_tag="VQU32_001394"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET2472835.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGITMTGF"
     gene            2796..5120
                     /gene="pflB"
                     /locus_tag="VQU32_001395"
     CDS             2796..5120
                     /gene="pflB"
                     /locus_tag="VQU32_001395"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HET2472836.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            complement(5487..5705)
                     /locus_tag="VQU32_001396"
     CDS             complement(5487..5705)
                     /locus_tag="VQU32_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472837.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(5801..6039)
                     /locus_tag="VQU32_001397"
                     /pseudo
     CDS             complement(5801..6039)
                     /locus_tag="VQU32_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6032..6316)
                     /locus_tag="VQU32_001398"
     CDS             complement(6032..6316)
                     /locus_tag="VQU32_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472838.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            complement(6928..7098)
                     /locus_tag="VQU32_001399"
     CDS             complement(6928..7098)
                     /locus_tag="VQU32_001399"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2472839.1"
                     /translation="MTKQVPKFTKDTAQLYTCKWLLYNKVTKMYDHTVVNHSVREYIT
                     DSISTNTIEETG"
     gene            complement(7748..8971)
                     /locus_tag="VQU32_001400"
     CDS             complement(7748..8971)
                     /locus_tag="VQU32_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET2472840.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            9139..10461
                     /locus_tag="VQU32_001401"
     CDS             9139..10461
                     /locus_tag="VQU32_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2472841.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEDFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            10463..12349
                     /locus_tag="VQU32_001402"
     CDS             10463..12349
                     /locus_tag="VQU32_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HET2472842.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYINRVFKTDIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRGHDFVP
                     ELRFEDFLDKELSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEKFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYLRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGEMLWKKTRSYEELIEEYFSALYGSNWQSVVEYLEKL
                     SSYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            12517..12861
                     /locus_tag="VQU32_001403"
     CDS             12517..12861
                     /locus_tag="VQU32_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2472843.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            12871..14283
                     /gene="lacG"
                     /locus_tag="VQU32_001404"
     CDS             12871..14283
                     /gene="lacG"
                     /locus_tag="VQU32_001404"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET2472844.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGTNMWDVYLQE
                     NSPFLPDPASDFYYRYEEDITLAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGEIGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSEDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAALNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            14353..16032
                     /locus_tag="VQU32_001405"
     CDS             14353..16032
                     /locus_tag="VQU32_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2472845.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMIAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGIIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QLISFVFLSLMLVVDILIYLPFCRAYDRQLLVKEDIASSNDIILEEDTSEIMPGEIDV
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLDIQIVATRGMEYIHLTKSPSKALQFVLEHYQAV"
     gene            complement(16158..17597)
                     /locus_tag="VQU32_001406"
     CDS             complement(16158..17597)
                     /locus_tag="VQU32_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET2472846.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(17601..18950)
                     /gene="trkA"
                     /locus_tag="VQU32_001407"
     CDS             complement(17601..18950)
                     /gene="trkA"
                     /locus_tag="VQU32_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HET2472847.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            19138..19986
                     /locus_tag="VQU32_001408"
     CDS             19138..19986
                     /locus_tag="VQU32_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HET2472848.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            20001..20549
                     /locus_tag="VQU32_001409"
     CDS             20001..20549
                     /locus_tag="VQU32_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HET2472849.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLVGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
BASE COUNT         6251 a         3505 c         4219 g         6650 t
ORIGIN      
        1 tgtcgaagag acagatctaa gagttcaaaa ccagctttat tatttgaaaa agtgagtggt
       61 ttaagaacag tttttcctgg aacattcaag gctgtaacat cgtgtttatt tttagcgata
      121 tcaatgccca cataaagcat gggagtacct ccagatatag tatttcaagt ctacttggtt
      181 atccacgaat tttttgcctt gttaccttag acgagatcaa acgtctatgc gttatcaaac
      241 tcattaccaa ttgaaacaaa agctgtggtt agagcctttc ggaaatcgtc aagcgattgg
      301 aggaaatgaa ctaatccaca gtggcttatt ccaagtatac cacttgggct ttggcagtag
      361 ctaactgcgc taattataat atagggagta atctatgtat cttattgaaa ttttaaaatc
      421 tatcttcttc gggattgttg aaggaattac ggaatggttg ccgatttcca gtacaggtca
      481 cttgatttta gcagaggaat tcatccaata ccaaaatcaa aatgaagcct ttatgtccat
      541 gtttaatgtc gtgattcagc ttggtgctat tttagcagtt atggtgattt attttaacaa
      601 gctcaatcct tttaaaccga ctaaggacaa acaggaagtt cgtaagactt ggagactatg
      661 gttgaaggtc ttgattgcta ctttaccttt acttggtgtc tttaaatttg atgattggtt
      721 tgatacccac ttccataaca tggtttcagt tgctctcatg ttgattatct acggggttgc
      781 cttcatctat ttggaaaagc gcaataaagc gcgtgctatc gagccaagtg taacagagtt
      841 ggacaagctt ccttatacga ccgctttcta tatcggactc ttccaagttc ttgctctttt
      901 accagggact agccgttcag gtgcaacgat tgtcggtggt ttgttaaatg gaaccagtcg
      961 ttcagttgtg acagaattta ccttctatct tgggattcct gttatgtttg gagctagtgc
     1021 cttaaagatt ttcaaatttg tgaaagccgg agaactcttg agctttgggc aattgttttt
     1081 gctcttggtc gcgatgggag tagcttttgc ggtcagcatg gtggctattc gcttcttgac
     1141 cagctatgtg aaaaaacacg acttcaccct ttttggtaaa taccgtatcg tgcttggtag
     1201 tgttttgcta ctttacagtt ttgtccgttt atttgtataa gaaaaacctt gaaggggcaa
     1261 ctcttcaagg ttttatactc ttcgaaaatc tcttcaaacc gcgtcagctt tatctgcaac
     1321 ctcaaaacag tgttttgagc agcctgcggc tagcctccta gtttgctctt tgattttcat
     1381 tgagctttaa aatccagtca tggtaatccc caataggcgg acacctcttt ctttcttgct
     1441 taattcttca tagagttgca gggctatttg gcttatctga ctagcatctt gtgttttttg
     1501 agcaagactt tttcgtttgg taagagttga aaagtcctcg tagcggattt tcaaaatgac
     1561 aatttttcca gctttttctt gttgatgtag attgagagcg actttttctg atagaagagt
     1621 cagctctttt ttgatatctt cctcagcaag gagaatcttc ccgtaggttt tctccttgcc
     1681 gattgattta cggatgcgat tggatttgac tggagagttg tgaatgccac gagcctttcg
     1741 atacagatca tagcctagtc taccaaaacg gtctattagg gttacctcag gaacttcaag
     1801 taaatcagca ccagtaaaaa cgcccatttg atgaagacgt tctactgtct tttttcctac
     1861 tccatgaaat ttggaaatat ccatttgttt gagaaaatcc tcagcctgtt caggtagaat
     1921 cactgtcaaa ccatgtggtt tttgataatc actcgccatt ttagctaaga atttgttgta
     1981 agaaacgcct gcggaagcag ttagatggag ttcttgccag atatcttttt gaatgaggcg
     2041 agcaattttg accgctgact tgataccgag tttattttct gtcacatcca aataggcttc
     2101 gtcaatgctc atgggttcaa tcaaatctgt atagcgctta aaaatagctc gaatctggag
     2161 tcccacagac ttgtatttct cataattccc tgagataaag acagcctggg gacaacgttc
     2221 ataagcttcc ttggaactca tggcagaatg gacaccaaaa gctcttgcct cataactaca
     2281 ggtagaaacg actccccgtc cacctgtttg ccgagggtcg cttccaataa tgacaggttt
     2341 tcctctgagt ttaggattat ccctgatttc cactgcagca aaaaaggcat ccatgtcaat
     2401 atggatgatt tttcttgaca aatcatttaa caaaggaaaa atcaacatgc ctagcacctt
     2461 tttatactct tcgaaaatct cttcaaacca cgtcagctct atctgcaacc tcaaaacagt
     2521 gttttgagca atctgcggct agcttcctag tttgcttttc gatttccatt gagtgttact
     2581 gcttattttc ttttattata cccttttttc tgaaaaaaag aaaaaaggac tttatttttt
     2641 caaaaatata atacagtttg aaataaaata tagactgttt tagaaaagaa agtgtaaaaa
     2701 tagggaattt tcacttgttg aaatcggtta ctatatggta tacttgtctt atgaatgtaa
     2761 cagatgactg ttactagaaa aaagaggaca ttaatatggt tgttaagaca gttgttgaag
     2821 cacaagatat ttttgacaaa gcttgggaag gcttcaaagg cgtagattgg aaagaaaaag
     2881 caagtgtatc acgatttgta caagctaact acacacctta tgatggagac gaaagcttcc
     2941 ttgcaggacc aacagagcgt tcacttcaca tcaagaaaat tgtagaagaa actaaagcac
     3001 actacgaaga aactcgtttc ccaatggaca ctcgtccaac atctatcgct gatatccctg
     3061 ctggatttat cgacaaagaa aatgaagtta tcttcggtat ccaaaacgat gaactcttca
     3121 aattgaactt catgccaaaa ggtggtatcc gtatggctga aactactttg aaagaaaatg
     3181 gatacgaacc agacccagct gttcacgaaa tcttcactaa atatgtaaca acagttaacg
     3241 acggtatttt ccgtgcctac acttcaaata ttcgtcgcgc tcgtcacgca cacactgtaa
     3301 ctggtcttcc agatgcatac tcacgcggac gtatcatcgg tgtttacgca cgtcttgctc
     3361 tttacggtgc agactacttg atgcaagaaa aagtaaatga ctggaatgca atcaaagaaa
     3421 tcgatgaaga aacaatccgt cttcgtgaag aagtaaacct tcaataccaa gcattgcaac
     3481 aagttgttcg cctgggtgac ctttacgggg ttgatgttcg caaaccagcg atgaacgtga
     3541 aagaagcaat ccaatgggtt aacattgctt tcatggctgt ctgccgtgtg attaacggtg
     3601 ctgctacatc tctaggtcgt gtaccaatcg tattggacat ctttgcagaa cgtgaccttg
     3661 ctcgtggtac atttactgaa tcagaaatcc aagaattcgt tgatgatttc gttatgaaac
     3721 ttcgtacagt taaatttgct cgtacaaaag cttatgacca attgtactca ggtgacccaa
     3781 cctttatcac aacttctatg gctggtatgg gtaacgacgg tcgtcaccgt gttactaaga
     3841 tggactaccg tttcttgaac actcttgaca acatcggtaa ctcaccagaa ccaaacttga
     3901 cagttctttg gactgacaaa ttgccataca acttccgtcg ctactgtatg cacatgagcc
     3961 acaaacactc ttctatccaa tacgaaggtg taacaacaat ggctaaagac ggatatggtg
     4021 aaatgagctg tatctcatgc tgtgtgtctc cacttgatcc agaaaatgaa gaacaacgcc
     4081 acaacatcca gtacttcggt gctcgtgtaa acgttcttaa agcccttctt actggtttga
     4141 atggtggtta cgacgatgtt cacaaagact acaaagtatt tgatatcgaa ccaatccgtg
     4201 acgaagttct tgaatttgaa tcagttaaag cgaactttga aaaatctctt gactggttga
     4261 ctgacactta cgtagatgcc ttgaacatca tccactacat gactgatagg tacaactacg
     4321 aagctgttca aatggccttc ttgccaacta aacaacgtgc caacatggga ttcggtatct
     4381 gtggatttgc taacactgtt gatacattgt cagctatcaa atacgctaca gttaaaccaa
     4441 tccgtgacga agatggctac atctacgatt acgaaacaat cggtgactac ccacgctggg
     4501 gtgaagatga cccacgttca aacgaattgg cagaatggtt gatcgaagct tacacaactc
     4561 gtctacgtag ccacaaacta tacaaagacg cagaagctac agtatcactt ttgacaatca
     4621 catctaacgt tgcttactct aaacaaactg gtaactcacc agttcacaaa ggtgtatacc
     4681 tcaacgaaga tggttctgtg aacttgtcta aacttgaatt cttctcacca ggtgctaacc
     4741 catctaacaa agctaaaggt ggttggttgc aaaacttgaa ctcactttct agccttgact
     4801 ttagttatgc agctgacggt atctcattga ctacacaagt atcacctcgc gctcttggta
     4861 agactcgtga tgaacaagtt gataacttgg taacaatcct tgatggttac ttcgaaaacg
     4921 gtggacaaca cgttaacttg aacgttatgg acttgaacga tgtttacgaa aaaatcatgt
     4981 caggcgaaga cgttatcgta cgtatctctg gatactgtgt aaacactaaa tacctcactc
     5041 cagaacaaaa aactgaattg acacaacgtg tcttccacga agttctttca atggatgacg
     5101 ccttggatgc attgagctaa tcaagttctt gaataataaa aaggaaccct cggtcaaacg
     5161 actgagggtt ttgtgcttgg gatagtatga gcaattcctt cggcgcaata tgcaatgttt
     5221 ttgggaagga atagtggacc gaatctgaaa tataatagat tgaaactaga atagtacacc
     5281 tttacttcta aaacattctt agaaatcgat ttgactgtcc tgatcgattt gtcctattct
     5341 tatttcattt tactatagtc cgctgaaacg gctcaaacag tgctagaaat tgactggaat
     5401 tctctaagtg atttgttccg attttatttc atttcatcat aaaaataaga tgactggcta
     5461 attgacaatc atcttgtttg atactgttaa taaaattcgc gaagtttatc ttgattttct
     5521 ttaaaaaatt tttctaacca atccatacca taagcataac taaaaccttc taatagaaca
     5581 gagaggtcaa tagtatcaaa tcccatgtct tttaaatatt taaaggtatc gaatggtgaa
     5641 tgaaagtttt tgtatttaat aattgcacca atatcttgca aatcctgctc tctgatactc
     5701 atcatcttca ttcctaacac atactcaata gggactacta aaacggtcag attctcaaat
     5761 gagtacaagg attcgcataa acttaaggga ggttgtttgt tcatatttgc tttgcaacat
     5821 gattgttgag ccataactct tcgtggatgt taagattaaa ctgttttcct acacgagcaa
     5881 taatttcatt gattttctga ttttggtcat aaaaagcatc aacatcttgt gtggcacgta
     5941 aaccatgata ttctaagaca taaccataat tgttaaagtc agattttcct gaattaattt
     6001 cttatttagg gcttggaaga ccggtttcat tttatccatg tttgtatttc tccatcaatt
     6061 tttgaacatt ggttaactta gcaggtttat ttaatgaaat cacatattta tgagttggag
     6121 tatgctcttg aaaggcatac ttcaagctaa ttttttgaaa acgggttagt tctttggagt
     6181 ctagcaactt ttctacatct tggtttgtta aataagaata gtcttcaaaa ataacttttc
     6241 gcaaatagcg aaaccaatgg cttgcgttga attgtgattg tttggatgcc tgtatcatat
     6301 aatcgtacca atccattgtc ttcctcctat aaaatttagt ttatattatt gtagtttata
     6361 ttcaaaaatg tagtttttct taattagctc gtatctatag gatgatactg aaaatcttct
     6421 tgttgcttta aatcacaatc gaaaaattct gtttttcatc ttagaaaaga atgtttagaa
     6481 atgtaaaatg aacctttttt cttatccgaa aaccttacta aagtatataa tttaggcagt
     6541 caattctgta tgataatgtt tgtactatcg tcgagttttt gcttgattgc ctaacgagat
     6601 aaattttaga agagttttcg gtgtgatttg acaagtttct gactggaagg atagttacac
     6661 tagtattgtt tattaaaatg agcagataga gaaaatttgg aatctatttg cttattatca
     6721 atcgtttttt tagcttattt gtcttagatt ggtatagaac tgagctatgg attaatcttc
     6781 ttctatctta tcctgttcct aaatatgttg gtagaaaaat gaagcagttg ttacgagtgt
     6841 ctgtattggg aaaaattttg tctatagaat tctttaaatg atatgaaggc taggaatgcc
     6901 aaaacactga catatttcgt ttaaagtcta tccagtctct tcaattgtat ttgtagaaat
     6961 gctatcagta atatattcgc gaacactatg atttacaaca gtgtggtcgt acatctttgt
     7021 aactttatta tacaacaacc atttacaggt gtaaagttga gcagtgtctt tagtaaattt
     7081 agggacttgt tttgtcatag ttttgatacc tgtattcgtt gtttcatgag catttgacgt
     7141 ttcttttcat gtaggcattt caacaattag aatgatattt ttccgaggat gaacttatat
     7201 attgtgactt tttagagtta tgatgcctct tgatttgact accgctatca atttcatcca
     7261 cttttacagt ttcatggttt tatgaaccga aacatctagg tacaggtttt agcatcaatt
     7321 gattgttttt gagccatatg aagttgaaac gtggagatgc ttttcttgtg ataacgccct
     7381 attcaaagag gaataaccgt ttttggagag agttcagtac tatgtggcgc taaatactat
     7441 atgtaaaaca tatattaaac aagatttaac taaggagaat tgtaaaaaca aaaatgagaa
     7501 tttcatataa agtttattct aggagtatgt atcttgaata aaaatcgtta tttaagttca
     7561 gggttatgat gaatgttttt ataataataa aatgcagcta gaagaaaagt tattttaata
     7621 taaatcactc aacatagaga gaaggcggtt tcctatatgg aagtcgtctt gttttcatat
     7681 tgtctaaata cgagatatct actacaggtt atagttgatt taaacaggtc tatagatttc
     7741 taatatatta aggtgaaata atatcttgta atacattgct atgttctatg aaaaaagcga
     7801 cgatagctaa tgcacaagct cctatacttc cacgatgttt gttgtaaggt aaaatttcaa
     7861 tttctatatt acgagtaaag ggaacgaagt ggagctggtc ttggatttta tccctgactt
     7921 catgagtgaa aggttgataa ttaagcaatt gactgttgat atagatttta tcagcaggat
     7981 tgatgaggag gagatttgca atagaagtgg ctaataaatt gactccttta tcaatttgtt
     8041 gacgtaaagc ggagtcgcct aaattataag ccgttaaaag ggtgtctaaa tgaatgtctt
     8101 tttcagtctt tacaaggctt tttagtacag ttagttggga atttttaaat aataattggg
     8161 cgtgtttgat taaccaagca tccgaaatat atgtttgtaa acaacctttt tttccgcatt
     8221 cacaatattg cccattcaat tcgacaatgg tgtgtccaat ttctccgata taaggatttt
     8281 tagaggctcc tattttttct tttgtgaaaa aggaagtgta aatacctaat ccagcgtgta
     8341 ggaaaataaa gttatcgggg gtattgtgtg gattaaaaag gtattgtcct atagccatac
     8401 aatcgatatt attttttaca ataaaaggaa aattgaatct tttaattaca tttaataaat
     8461 taaaagattc ccatatgggg ttgtttgtaa tgatacttcc ggagtctttg ttataatgtc
     8521 caggtattga taagccaaat ccaagtaatt ttgtttccaa agggacgtac tctatagctg
     8581 tttttatcat attggtaatg gtggaattga tttctgaaat aggataatct tcattcaaaa
     8641 tttctgtctc gaaaagagtt ttcccttgta agtcacatag actgagaaag atacctagga
     8701 cgtttatttc aatacctcca acaacatacg gaatattatc tgtgatagaa atagtttttc
     8761 tacgtcttcc agatccggaa aactcgctaa attcatcacc agtttcgtat atgagggact
     8821 gttcgcttag ctcttttatg atgttagtcg ttgttgctgg agtaagtcct gtttcattaa
     8881 caatatctgt acgggatgtt gctggatttc gatatagaaa ctccagtata gtggctctat
     8941 tttgtaaaaa ggctgattgt tttgtaatca tttgtttcct cctttggttt attttatatt
     9001 aaacataaga aaaaatcaat taaaattatt attttataca aagaaaaaaa tattgtaaaa
     9061 acaacaaaaa atatattgac aacgctttct gttagtggta tatttaattt ataaaaaata
     9121 aaaaaaggag ttccggtgat gaacaatatt ttagcgtttt tagaaacaaa agtcgctccg
     9181 tttggtgaaa aagttggcaa ccaacggcat ttgaaagcta ttcgtgaagg atttatgatg
     9241 gcaatgcctt tgattttagt cggatcttta tttcttattc taatcagttg gcctcaagag
     9301 gattttacaa attggctgaa tagtgttgga ttgctaagta tcttgacaac tatgaatcag
     9361 tcaacagtag cgattatctc cttggtcgct tgtttcggta ttgcctacag gttgtcggaa
     9421 ggatatggta cagatggtcc gtcggcaggg atcatagcct tatccagttt tgtattgatg
     9481 gcacctcgtt tttcgagtat ggtttatgat aaaaatgggg agcaggtcaa gcagttattt
     9541 ggcggcgcaa taccattttc tagcctgaat gcatcttctt tgtttatggc gattactatt
     9601 ggattggtta cagcagagat ttatcgtatg tttatccagc gcggaattac gataaaaatg
     9661 ccaagtggtg tcccagatgt ggtaagtaaa tcattttcag ctcttttacc tggctttact
     9721 acttttgttt tgtgggctct ggtcttaaaa ggtcttgaag cggcaggagt tgcaggaggt
     9781 ctcaacggac tcctaggtgc aattgttgga acaccgctta agttaattgc aggaacgctt
     9841 ccaggtatga ttctatgtgt tattgtaaac tcattctttt ggttctgtgg agttaatggg
     9901 ggacaagttt taaatgcttt tgtagatcca gtttggttac aatttactac agaaaaccaa
     9961 gaagctgtgg ctgcaggaca aacactccaa cacattatta cattaccgtt taaagattta
    10021 tttgtattta ttggtggcgg tggagcgact attggtcttg cgatttgtct cttcctattt
    10081 agtaagagtc gtgcgaataa aacattaggt aagctagcta ttataccgtc tatttttaat
    10141 atcaatacag ctattctatt tacgtttcca acagttttaa atccgattat gctgattccg
    10201 tttattgcta ctcctacaat caatgcctta attacctatg tatcaatggc tgtaggatta
    10261 gtaccctaca caacaggtgt aatccttccg tggacaatgc caccgatcat aggaggcttc
    10321 cttgcgacag gagctagttg gcgaggagct ctattacaag ttgttttgat tttggtttct
    10381 gtagcaattt attatccatt cttcaaaatt gcagataaac gcaatcttga aaaagaaaaa
    10441 gctactgttg gagggaaata agatggttat cagagtattt gatcagcaga aaaatactta
    10501 ttctagcttt gccttagagg aattaagtta ctatataaat cgggtcttta agactgacat
    10561 agaacttgtc gaggagaagg aagcggatat ttttgtagga ttagtcaata aagaggatag
    10621 aaaagaccat gttcttatct cattagacaa gggtaagggg agaattgagt ctaatacaat
    10681 tgtaggtttg cttattggaa tttaccgaat gtttcatgaa tttggggttg tgtatactag
    10741 accagggcgc ggacatgact ttgttccaga gttacgattt gaagattttt tagataaaga
    10801 gctatctata gatgaaacag ccagttacta tcatagggga gtatgtatag agggagcaga
    10861 ttcatttgaa aatatactag atttcattga ttggctacct aagattggga tgaacagttt
    10921 tttcattcag tttgaaaatc cctattcttt tttgaaacgt tggtatgaac atgagtttaa
    10981 tccataccta aataaagaaa aattctcaaa tgaattagta caagaattga gtgataggtt
    11041 ggataaagaa ttgcaaaaaa gaggtcttat tcatcatcgt gttggtcatg gatggacagg
    11101 tgaggtttta ggttactctt caaaatttgg ctgggaatca ggtcttagta tttcagagga
    11161 gaagaaaccc tatgtcgctg aaataaacgg gaaacgagaa ttatttaata cggctccgat
    11221 tttaaccagt ctggattttt caaatccaga tgtggcggat aagatggtag aaattatcaa
    11281 ggattatgcc aagaaaagac ctgatgttaa ctacttacat gtatggttgt cggatgctcg
    11341 taataatatt tgtgaatgcg aaaactgtag acaagaattg gtttcggatc agtatattcg
    11401 tattctcaat caattggata gggctttaac gagtgaggga ttagatacaa agatttgttt
    11461 tctgctttac catgagttgt tatgggcgcc tcagaaagaa aaattagata atcctgaacg
    11521 ctttaccatg atgtttgcac cgattacaag aacatttgaa atgagttatg cagatgtaga
    11581 ttttgacaat tccataccta cgcctaaacc ttatctgcgt aataaaatta tacttccgaa
    11641 ttctcttgag gaaaatttat cttatctttt tgagtggcaa aaaacattta aaggagatag
    11701 tttcgtatat gactatcctt tagggcgtgc tcattatggc gatttaggct atatgaaaat
    11761 tagtcaaacc atttacagag atgtatctta tctttctaac ctacatttga acggttacat
    11821 ttcgtgtcaa gaattacgtg ccggattccc tcataatttt cctaattatg ttatggggga
    11881 aatgctctgg aagaagacaa gaagttatga agaattgatt gaagaatact tttctgcttt
    11941 gtatgggtca aattggcagt ctgttgttga atatttagaa aaattatcca gttattcctc
    12001 atgtgattat tttaatgcaa ttggcagtcg tcaaaatgat gttttagcga atcattatta
    12061 tatagcttac aatctagctg ataatttttt gccaattatt gaggaaaata tttctaagtt
    12121 attaaatagt caaaaggatg aatggaaaca gctcagttat catcgtgaat atgttgttaa
    12181 gatggcgaag gctttatatc ttcaagcaac cggaaaaaca aggcaagctc aagatgaatg
    12241 gagaaatgtg ttgaattata tccgtgggca cgaattgcta tttcaatcta atttggatgt
    12301 ttatcgtgta attgaagtag caaaaaatta cgctggtttc cacttataaa tcataagtat
    12361 agaaaatgaa ccaaggtatt cagagaagat tgatcctaaa tattatgaaa tttaaggatt
    12421 tttaagatat ttagggtcga ctttctattt atatcgtagc gaagtcattt taataatgat
    12481 gtgtaaaaga tggatcaaga ttgaggagga agaaagatga aatcaaaaga agaaataaat
    12541 atgcttggtt ttacaattgt cgcttacgca ggagatgcaa ggtcagattt gatggatgct
    12601 ttggcgtttg cgagagatgg atattttgaa caggcaagag aattggttga gtctgcaaac
    12661 gactcaatag tgtctgccca tcgagaacag actaatttat tagcggagga ggcatctgga
    12721 gataattttg aagtgagttt tatcatgatt catggtcaag ataccttgat gacaacgatg
    12781 ctattgtatg atcaggtcaa gttttttatt gatgaatatg aacgaattcg aaagattgaa
    12841 gaacatattg gtttgcaatg aggattagtc atggaaaatt tacaggttaa agccttaccg
    12901 aaggagtttt tattaggagc tgctaccgct gcttatcaag tagagggtgc aactagggta
    12961 gatggcaaag gaacaaatat gtgggatgtt tatttgcaag aaaacagtcc gttcttacca
    13021 gatccagcta gtgattttta ttatcgttac gaagaggata taactttggc ggcagaacat
    13081 ggtttgcagg ctttgcgttt atctatttct tgggttcgta tatttcctga tatagatggg
    13141 gagcctaata tattagctgt tcgttattac catagagtct ttcagtcttg cttaaaacat
    13201 aatgtgattc cgtttgtttc tttacatcat tttgattcgc ctcagaaaat gttagaaaca
    13261 ggggattggt tgaatagaga gaatattgat cgtttcatac gatatgctcg cttttgtttc
    13321 caagaattta cagaagtcaa gcattggttt acaatcaatg aactgatgtc tcttgctgca
    13381 ggtcaatata taggaggtca gtttcctcca aatcatcatt ttcaattatc tgaagcaatt
    13441 caagcgaatc ataatatgtt gttggcgcat gctcttgcag tcctcgaatt tcatcaatta
    13501 ggaattgagg gagagatagg ttgtattcat gctttaaagc caggctatcc tattgatggg
    13561 caaaaagaaa atattttggc agctaaacgg tatgatgttt ataataataa atttctatta
    13621 gatggaactt ttttgggcta ctacagtgag gacacgcttt ttcacttgaa tcaaatattg
    13681 gaagctaata attctagctt tattattgaa gatggtgatt tagaaattat gaagagagct
    13741 gcagctctta atacgatgtt tgggatgaat tattatcgtt cagaatttat tcgtgaatac
    13801 aaaggtgaaa atagacaaga atttaattca acaggaataa aaggacagtc ttcttttaaa
    13861 ttaaatgctc taggtgaatt tgtaaaaaaa cctggtattc cgacaacaga ttgggattgg
    13921 aatatttatc ctcaagggtt atttgatatg ttgcttcgta tcaaagaaga atatcctcaa
    13981 catccggtca tttatttaac tgaaaatggt acagccctta aagaagttaa gccagagggc
    14041 gagaatgata ttattgatga cagtaagaga atccgttata ttgagcaaca tttacacaaa
    14101 gttttagagg ctcgagatag aggagtcaat attcaaggct attttatatg gtctttgcaa
    14161 gatcaatttt cttgggcgaa tggctacaat aagcgatatg gtcttttctt tgttgattat
    14221 gaaacacaga agagatatat taagaaaagt gctctttggg taaaagggct aaaacggaat
    14281 taaggttagc gatttgactg atgtttaata tattttaact atgagcttaa attttttaca
    14341 ggaggagttt ttatggataa gttagtcgct gccattgaaa agcaacaagg gaaatttgaa
    14401 aaaatttcta ctaataatta tatgatagct attaaagatg gatttattgc tactatgcct
    14461 ttaattatgt tttcaagctt tttgatgatt attattatga ttcctaaaaa tttcggagta
    14521 gagttaccga gtccagctat tgcctggatg aggaaagtgt atatgctaac catgggagtt
    14581 ttgggtatta ttgtttcagg gacagttgca aagtcattgg ctggaaatgt taatagaaaa
    14641 atgcctcctg gaaaggtaat aaatgatact tctgcaatgt tggcggccat atgtggctat
    14701 cttgtattaa ctgtaacgct tgtagttgat gagaaaacgg gatctacaag tttgtcgaca
    14761 aactatttag gatctcaagg attgataact tcgtttgtca gtgcctttat tactgtaaat
    14821 gtttaccgat tctgtattaa gcgagacatt actattcatt tacctaagga agttcctggg
    14881 gctatatcac aagcttttag agatattttt cctttttctt ttgttttact tattagtggt
    14941 ttgttagata ttgtatctcg gtttagttta gatgttcctt ttgcccaagt atttcaacaa
    15001 ctattgactc ctatttttaa gggggcagaa tcatatcctg ctatgatgtt gatttggttt
    15061 atgtgtgctt tgctttggtt tgttggaatt catggaccat ctattgtctt acctgctgtt
    15121 acagctttac aactgagcaa tatggaagag aatgctcaac ttcttgcaaa tgggcagttc
    15181 ccttatcatt ctttaacacc taatttcggg aattatatcg ctgctattgg aggaacgggg
    15241 gctacctttg ttgtaccatt tattttgatt ttctttatgc ggtctaaaca attaaaatcg
    15301 gtaggtaaag ctacaattac tcctgtttta tttgcggtaa atgaacctct tctatttggt
    15361 atgcctgtta ttttgaatcc ctatcttttt gtcccttttt tgatgactcc accagtgaat
    15421 gtatttctag gaaaggtatt tattgatttc tttggaatga atggatttta tatccagtta
    15481 ccttgggcct ttcctggtcc cttgggattg ttaattggaa cgaattttca acttatctcc
    15541 tttgtatttt tatctttgat gttagttgtc gacatattga tttatttgcc attctgtaga
    15601 gcgtatgata gacagttact ggtgaaagaa gatattgcaa gctcaaatga tattatttta
    15661 gaggaggata caagtgaaat aatgcctggt gagatagatg taataaaaag taaggagttg
    15721 aaagtactgg ttctttgtgc agggtctgga acaagtgcgc aattagccaa tgcaattaac
    15781 gagggcgctc aattagcaga agttagagtg attgcgaatt caggagcgta cggagctcat
    15841 tatgatatta tgggtgttta tgatttaatt attctggccc cacaagttcg gagttattat
    15901 agagagatga aggtggatgc agaaagatta gatattcaga tagttgctac cagaggaatg
    15961 gaatatattc atttaacaaa gagtccaagt aaagccttac aatttgtatt ggagcattac
    16021 caagctgtgt agtaagtttt tccatctttt atttgagtaa agattttgct tacagatagg
    16081 cttggattta aaaacgttcc ccctttttta atataagaat ccctctttca caattgtaaa
    16141 aagagggatt ttgtatttta tctcttagac caagttctct tcataaagag aaggaggatt
    16201 gggtaaatct ccaagcgccc tgcaatcatt gcaaaggata ggagaatttt tgagatggga
    16261 ctaaagattg agaaactaga agtggttcct agaataggcc cgatattatt gaaacagcta
    16321 aagacagcgc tggtcacgac cagaaaatca ttgctatcta ggctgacaat aaagataagc
    16381 gctagcaaaa tcatagcata gatgacaaag tacttgagaa tcttatgctg ggtatctttg
    16441 tcaatcaccg ttttattaac atggagggtc aaaacacggt ggggcgatag gattgacaaa
    16501 atttggtttt tggcaatttt tgaaaggatg aggcctcgaa taatcttgag tccacctgca
    16561 gttgatccag cagagccacc gattgccatg aggaaaagga ggataaactg ggagaagagg
    16621 ggccagttgg taatatctcc atatccaaaa ccagttgttg taatgatgtt ggaaacctgg
    16681 aagaaggtca tttcaaagct ctttgaaaac cctgggtaga ggtagagggt gttgaggcta
    16741 atcaagcctg tagaaaccag tacaatgacc aagtaagccc taagctcttc atctccaaag
    16801 aaggccttga tgcgacggag catgaggtag tagtagaggt tgaaatttac tccaaaaacc
    16861 agaactccga tactgaccag ataggtaatc agtgagctgc catagtgggc aattccgtcg
    16921 ttatagacgg taaagcctcc agttcccgct gtccccatag caatgacaaa actatcgtag
    16981 agaggcatac cagctagata atagatgatg acaaagaggg agaagagagc tagataaagg
    17041 agatagagaa tctgggcagt gttttttagt ttggatacaa ccttgccaaa aacaggacct
    17101 ggaacctcag ccttcatcac ctctaggtgg ctatttttgg cattgtccat aatagcaagt
    17161 gcaaaaacaa gcactcccat ccctccaatc aagtgggtaa aacttcgcca gaagaggagg
    17221 gaacgactga gaactgaaac gtcgttcaaa atacttgctc cagtagtcgt aaagccggaa
    17281 ctaatctcaa aaaaggcatc aataaggctg gggatttgcc cagaaaagac aaaggggaga
    17341 ccaccaaaga aagaccaaag gatccaacag agggcaacga tcaagactcc ctccttggca
    17401 taaatccgtt gattttttgg cttctgtaaa ctccctgaac cgcctaacaa tacgagaatc
    17461 cctatggtcg aaaagagggc tgtaaagact tggctcgatt cacggtaata gacagcgata
    17521 gccacaggaa ccaagaggag aacagcttca atcaaaagta actttgaaag gaggtaacga
    17581 atcatacttt tattcatttc ttacctcgca atcaagtcat aaatcttggt gatgtttggc
    17641 aacaaggttg ttactaggag cttgtctcca acttccaaca tatcctcccc agttgggaaa
    17701 atagtcttgc cctttcgaat aatggctgca ataagaaccc cttttttcaa tttcagttga
    17761 gaaagaggtt tggcagtcat tttattggct tccttgatat ggaattgcag ggtttcgatt
    17821 tggccattgg ctagatggtg catggcttga aggtctgaat actgggcatt aactcgacca
    17881 cgaataaagt gcataatcgt atctacagcg atgcttttag gtgtgatgat acttgaaaaa
    17941 tcaggcgcat tgataatctc gaggagactg gtacgattga ccttagtaat atttttctgt
    18001 acacctaccc tgtcaaggaa catagatgta atcagatttt cctcatcgac tcctgttaga
    18061 gtcgcaacgg catcatagtg ttgagcactt tcttccagca ggatatcttt tgcggttcca
    18121 tctccttgaa cgatgtagag atttgggaat ttctcgctaa agaagctggc gatttcagga
    18181 ttgatttcaa tgacttttgt atcgatacga ctatctttga gaataccaag tagataatag
    18241 gcaattctac ctgccccaac gatgagaagg ctcttcacgg cgcgtgattt aaaataatta
    18301 tggaagagta tcatatcgac acggttacca gtgacaaaga ttctatcttt atcctgtaca
    18361 gtcatgtcac cgcttggaat gataatttga tgatccctct ctatcgcaca gacaatgaca
    18421 ttaccaaatt ttttacgaaa atcagaaatg ggcatttggc aaagaccgct ggtggacttg
    18481 acgacaaatt ccatgaggct aacgcgtcca ccagcaaagc gttcgacaga cagggcgttg
    18541 gggaagtcaa tgatattcgc gatagcacgg gcagccaaga gctcaggatt aacgataaga
    18601 gaaaaaccga gaatattctt ttccttgaaa taagagttag aatattcagg gttccgcacc
    18661 cgaacgatag tttctttagc tcccattttc ttggctagaa ctgctgcaat catgttgact
    18721 tcatcgtgct cagtcagggc gataaagata tcacaatctt ggacgctggc ttgctcaaga
    18781 atggcaaaat cggccccgtt accaaggata ccaatgatat caaagcgact gacaatatga
    18841 ttgagaacag cttcgtcttg ctcaatcagc aaaacatcat gcttttctgc aaccaaggag
    18901 cgacagaggg caaaaccaac ttttccccct ccgacaagga taattttcat aataaaacct
    18961 actttttcat gatgtaacta tcataccctt tttcaagaaa aaatgcacct actagctaat
    19021 aacaagagct tttagtgaaa attcgctata agataaaact ataccctaac caattgaaat
    19081 agctattagc gactttctct gaaatatggt atgataaagg atatacaagg agataaaatg
    19141 aataataatt tactggtatt acaatcagac tttggtctgg ttgatggtgc ggtatcggct
    19201 atgattggag tggctttaga agagtctcca accttaaaaa tccatcactt gacgcacgat
    19261 atcacgcctt ataatatttt tgaggggagc tatcgtctct ttcagacggt ggattactgg
    19321 cctgagggaa cgacgtttgt atcggttgtc gatccaggtg tcggttcgaa acgtaagagt
    19381 gtagttgcca agactgcaaa aaatcaatac attgtcacgc cagataatgg gacgctttcc
    19441 tttatcaaga aacacgttgg cattgtagcc attcgtgaga tttctgaggt ggccaatagg
    19501 cgtcaaaaca cagagcattc ttataccttc cacggtcgtg atgtctatgc ctatactggt
    19561 gctaaactgg ccagtggtca cattactttt gaggaagtag ggccagagct cagtgtggaa
    19621 cagattgtag agcttccagt cgtagcgacc atcatagaag atcatctggt gaagggagcc
    19681 attgatattc tggatgtgcg tttcggttcg ctttggacct ctatcacacg ggaagaattt
    19741 tacaagttgg aaccagaatt tggtgatcgt tttgaagtga ccatctatca tgctgatatg
    19801 ctggtctatc aaaatcaggt tgtctatggc aaatcatttg cagatgtgag aattgggcaa
    19861 cccatccttt acatcaactc tctctatcgt ttaggtttgg ctatcaacca aggttccttt
    19921 gccaaggcct ataatgtagg tgtcggttca tcttggacca ttgaaataaa gaaaatagaa
    19981 ggataaaata ggagaatagt atggaaatca aatttacaat taaacaagtt gttgctgtcg
    20041 gaattggcgc tgccctcttt gtcgtcatcg ggatgatcaa cattccaacc cctgttccaa
    20101 atacaagcat ccagcttcag tatgcggtac aagcgctact ttctattatt tttggaccga
    20161 ttatcggttt gcttgttggg ttgattggtc atgcaatcaa ggactctctt gttggttatg
    20221 gtctatggtg gacttggatt atcgctagcg gactctttgg tttagttgtg ggacttttta
    20281 gaaagtacgt tcgcgtgatc aatggtgttt ttgactggaa agatattctt atttttaacc
    20341 tcattcaact acttgcaaat gcccttgttt ggggtgtctt ggcaccactt ggagatgttg
    20401 tgatttatca agaagcggca gaaaaagtat ttgcacaagg aattgttgcg ggaattgcca
    20461 atggtgtatc tgtagctatt gcaggaactc ttctcttact tgcctatgca ggaacccaaa
    20521 ctcgtgcagg aagtttgaaa aaggactaat actcaatgaa aatcaaagag caaactagga
    20581 agttagccgc aggttgctca aaacagtgtt ttgaaattgt ggata
//
LOCUS       DAVBBS010000040        20486 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVBBS010000040 DAVBBS010000000
VERSION     DAVBBS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20486
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.040"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..128
                     /locus_tag="VQU32_001410"
     CDS             <1..128
                     /locus_tag="VQU32_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2472850.1"
                     /translation="FRSLENLEQAIVDYIDYYNNKRIKVKLKELSPVQYRTKSFG"
     gene            complement(176..2719)
                     /locus_tag="VQU32_001411"
     CDS             complement(176..2719)
                     /locus_tag="VQU32_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HET2472851.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQKKVD
                     ILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTKTFT
                     EAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQAQT
                     GFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(2781..4403)
                     /locus_tag="VQU32_001412"
     CDS             complement(2781..4403)
                     /locus_tag="VQU32_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2472852.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            4609..5634
                     /gene="trpS"
                     /locus_tag="VQU32_001413"
     CDS             4609..5634
                     /gene="trpS"
                     /locus_tag="VQU32_001413"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET2472853.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            5787..7265
                     /gene="guaB"
                     /locus_tag="VQU32_001414"
     CDS             5787..7265
                     /gene="guaB"
                     /locus_tag="VQU32_001414"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET2472854.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(7316..8413)
                     /gene="recF"
                     /locus_tag="VQU32_001415"
     CDS             complement(7316..8413)
                     /gene="recF"
                     /locus_tag="VQU32_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HET2472855.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(8416..8784)
                     /gene="yaaA"
                     /locus_tag="VQU32_001416"
     CDS             complement(8416..8784)
                     /gene="yaaA"
                     /locus_tag="VQU32_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HET2472856.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKIDIPDMNIDILLTQSTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            8943..10193
                     /locus_tag="VQU32_001417"
     CDS             8943..10193
                     /locus_tag="VQU32_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET2472857.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSYLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHCPSKYGDEKHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSQLDLFSGFLRMYAGIDRENRNQARKMMNNQLLDL
                     KKGYFTELELEQTKEMIRRSLLLSQDNQSSLIERAYQNALLGKSSADFKSWIAKLEQV
                     DKDAICRAANNVKLQAIYFMEGIE"
     gene            10190..11473
                     /locus_tag="VQU32_001418"
     CDS             10190..11473
                     /locus_tag="VQU32_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET2472858.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTLVTEVDGDVKQYPAGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELITSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKLTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            11515..12336
                     /locus_tag="VQU32_001419"
     CDS             11515..12336
                     /locus_tag="VQU32_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2472859.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            12347..12892
                     /gene="pgsA"
                     /locus_tag="VQU32_001420"
     CDS             12347..12892
                     /gene="pgsA"
                     /locus_tag="VQU32_001420"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET2472860.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            12889..13716
                     /locus_tag="VQU32_001421"
     CDS             12889..13716
                     /locus_tag="VQU32_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET2472861.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            13701..14540
                     /locus_tag="VQU32_001422"
     CDS             13701..14540
                     /locus_tag="VQU32_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET2472862.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPNQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            14533..15327
                     /locus_tag="VQU32_001423"
     CDS             14533..15327
                     /locus_tag="VQU32_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2472863.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            15386..16204
                     /gene="mreC"
                     /locus_tag="VQU32_001424"
     CDS             15386..16204
                     /gene="mreC"
                     /locus_tag="VQU32_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET2472864.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKVSQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            16204..16698
                     /gene="mreD"
                     /locus_tag="VQU32_001425"
     CDS             16204..16698
                     /gene="mreD"
                     /locus_tag="VQU32_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET2472865.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            16792..17970
                     /locus_tag="VQU32_001426"
     CDS             16792..17970
                     /locus_tag="VQU32_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HET2472866.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            18194..18973
                     /gene="rpsB"
                     /locus_tag="VQU32_001427"
     CDS             18194..18973
                     /gene="rpsB"
                     /locus_tag="VQU32_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET2472867.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            19052..20092
                     /locus_tag="VQU32_001428"
     CDS             19052..20092
                     /locus_tag="VQU32_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HET2472868.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
BASE COUNT         6528 a         3272 c         4230 g         6456 t
ORIGIN      
        1 gctttagatc tttagaaaac cttgaacaag ctattgtgga ctacattgat tactacaaca
       61 acaaacgaat taaggtaaaa ctaaaagaac ttagccctgt gcaatacaga actaaatcct
      121 tcggataaat taattgtcta actttttggg gtcagtacat taactggtcg attttttatg
      181 ccttagatga ctttcgtctg tgattataga ttccaaatag taagagagaa gtaaaggaac
      241 agattgctcc agtaataaaa ccattgggaa tgaaggaaag tgtaatagtt cctttcccct
      301 tgggaatgtc aactttcata aatccagttt gagcttgttt aatttctatt ttcttaccat
      361 cttggtaggc agaccaacct ttgtcataag gaatggtgaa gaaaatagat gtatcttgtt
      421 ggacatcata tgtagcaaaa accttgtttt tagaagttga tactgtgaca ggttgttctt
      481 taattttttg aattgcctcg gtgaaagttt tggtatctaa acgatagaag gtaggagatt
      541 caaatgatac ttgtgaattt ccagggaaac taacattgat attgaaagtt tttttctctt
      601 tagtatatcc tagattaaag aaggagaaga cattatcagt tgtaaaagtc tttttttcac
      661 catttacaag gatgtcaacc ttcttttgtt tatcgttaga aaagtgaagg tttatgaaag
      721 agagataaac ttggctgttt tctggaactt caatttgata ctggattgct gcatcttcat
      781 ttgaagaact tgtgacacta atcaaatcat tagtattttc tgttttttca taaggtattg
      841 gagaaaaata atcaaaattg acgttagcaa gttgatttaa aaatgaggcc tgattatcca
      901 aggtatgttc attgaacttg acatcattgt aaacagattg actagcaact gcaatcggaa
      961 gagagtattg attttcatat agggtaagat tatctttttg atagatatct ttaaagccat
     1021 acttatcaat aggactgtct gagatattgt actggatacc aaataaacta tcagccaaaa
     1081 tactattatt tgcatatcgg agattgagat tagtcccaga ggatttaaaa ccaagtttat
     1141 ctaaagtaga gcttgatgaa cgatttcgaa cagatgaaaa ttgagagatt ccattgtagt
     1201 tgaatttcat actgtcattt cctgtctgag tttgtagttt ttcagtacga gtaaattgat
     1261 ttccaatata tgttgagaaa gattccatag ctgggatatc tcgactataa gcacttcgag
     1321 aagcaaatcc ccattcctta gcaattccgt ccatttgaga tgaagcattt aaactcattt
     1381 caaccagtat aaataaagag attagaatgg caaatagatt cacagatata aactttttga
     1441 taactgcaag gagtaaaaga gaatagacaa ccaaaaattc aagagtaagc agaatattca
     1501 aatctgttaa aaaagaataa tgcgatttta gatagatggt agctaaaaat cctgctacta
     1561 caagaaaaag cgaaactaaa aaattccaga ctttaagttc tttcagacgc tttaagactt
     1621 ctgctgctgt gtaaattaac aaggtagaga aaatccaagc atagcgatgt aaaaacatgt
     1681 ttggagtatg catgccttgc caaaataagt caagagcttc tatgtaaaag cttgcaatta
     1741 gaaatgcaaa gaatattaca tatatgagtt tcacgtgaaa cttaatagat ttcagcgtaa
     1801 aaaataaaat ggtcaaaata aagggaaata gtccaacaaa aatcattggg atggccccat
     1861 actttgttgt gtcaaaggaa ccaatgaatt gcttagcaaa gagatcaaga taccagctac
     1921 tttcagtttg aaactttgta acttcagtca atttttcccc atgtgtctgt aaatcaaata
     1981 gagtgggaag agtcataatc aaactagcca taccagctaa aaaggagata actatgaaat
     2041 caagaacaga tgattttcga gtcttaaagt cccacgaaat ttgacagaga taccagaaaa
     2101 taagaaacaa tactgtcata tatccaaaat aataattttg aataaataag attgacagac
     2161 ttgtaaagta caataggagt ttcttttcag ttatcagtag atgtaaacca gttataatta
     2221 aaggaatcaa gataaaaaca tctagccagg tttttatctc taattgactg acagtgaaac
     2281 tcatcagagc ataggaagta gataaggcta gttttaaagt ctgagggata gattgaaaca
     2341 atttattcaa actaaaaaag gttgacagac caatcaatcc aaattttaag agagtggtca
     2401 gatagacagc atctggcata ttcgttagat caaaaaagta aactagaggc gcgagaaaac
     2461 tacccaagta ataactagat agggcataga agtttagccc tagaccactt gtaaaggtgt
     2521 aaaacagact actatttcca tgtaggatat ttcgtaaggc tacatcaaaa ataacgtatt
     2581 gatgaaagcc atctcctaat agaggagagt tgtcgctatt ccagtagata ccttgagata
     2641 gatatactcc agacataatc actacaggaa tgatgaaaga aataaaatag gttcgatatg
     2701 tttttaaaaa tgatttcatg ttacctcgta gaatgataga aaactcagtt ggttaaccca
     2761 actgagtttt gaagttttat ttagtctttc caaagttctt taacttttgc ttgtacttct
     2821 gcattttcta ggaattcatc gtaggtttca tcgatacggt caatgacgcc atttttagat
     2881 aagacaatga tatggttagc caaagtttga ataaactcgt ggtcatggct ggcaaagatg
     2941 attgattctt taaagttttt cagtccatca ttcaagcttg agatagattc caagtccaag
     3001 tgatttgttg gatcatcaag tacaaggaca tttgatttta agagcatgag ttttgaaagc
     3061 atgacacgaa ctttttctcc ccctgacaag acatttacag gtttgttaac ttcatctcca
     3121 gagaagagca tacggccgag gaaaccacgt aggaaagtat tgtcatcttc ttctttactt
     3181 gcgaattgac gcaaccagtc aagaattgat tctcctcctg caaaatcagc tgagttatct
     3241 tttggtaggt aagattgact agttgtaact ccccacttga cagttccttc atagtcaata
     3301 tctcccatga ttgcacgaat taatgcagtc gtttgaatat cattttgtcc aataagtgct
     3361 gtcttatcat ctggacgcaa gatgaaacta atattatcca agatagtttc accatcaatc
     3421 tttacagtta gattttctac tgtcaagaga tcattaccaa tctcacgttc cgctttaaag
     3481 ttgataaatg gatatttacg actagatggt acaatctctt ctagctcaat cttatcaagc
     3541 attttcttac gtgatgttgc ctgccttgac ttagaagcat tggcagagaa acgagcaacg
     3601 aattcttgca attgtttaat tttttcttct gctttagcat tacggtctgc tagcaattta
     3661 gcagcaagct cagaagattc cttccagaag tcgtagtttc cgacatagag tttgattttt
     3721 ccaaagtcaa ggtcggccat gtgagtacaa actttgttta agaagtgacg gtcgtgggat
     3781 actacgataa ctgtgttatc aaagtcaatc aagaagtctt ctaaccaagt aatcgattgg
     3841 atatccaaac cgttagtagg ctcgtccaag agaagaacat ctggtttacc aaaaagtgct
     3901 ttggcgagga gaacctttac tttttcaccg ttggccaatt cgctcatgtt ttggtagtgt
     3961 aattcttctg gaatgtttag gttttgaagt agttgagagg cttcactctc tgcttcccaa
     4021 cctccaagtt cggcaaactc tccttcgagt tcggcagcac gaaccccgtc ctcgtctgag
     4081 aaatcttcct tcatgtagat agcatctttc tctttcatga tgctataaag tttttcattt
     4141 cccatgataa cgacatcaat ggcacgttca tcttcgtagt caaagtgatt ttgacgaaga
     4201 acagagagac gttcatctgg accaagagag atgtgaccag tagtaggttc gatatctcca
     4261 gctaaaattt ttaaaaaggt tgattttccg gcaccattag caccgattaa tccgtaagta
     4321 tttccttctg taaatttgat attgacatca tcaaaaagtt tgcgatcact aaaacgtagt
     4381 gaaacatcag atactgtaag caatgttttt ctcctatatg tgtaatatat ttattctact
     4441 agaaaataca gaaatattca aatttttatt tgtcaatttt gtgtaaatta tatttacagt
     4501 atcctttaca caaatctgta aaaagcaagg ctgatttatt ttgataaatt acggttattt
     4561 cattaaaaaa atgctataat tgaaaggact atatcgaagg agaacaaaat gactaaaccc
     4621 attattttaa caggagaccg tccaacagga aaattgcata ttggacatta tgttggaagt
     4681 ctcaaaaatc gagtattatt acaggaagag gataagtatg atatgtttgt gttcttggct
     4741 gaccaacaag ccttgacaga tcatgccaaa gatcctcaaa ccattgtaga gtctatcgga
     4801 aatgtggctt tggattatct tgcagttgga ttggatccaa ataagtcaac tatttttatt
     4861 caaagccaga ttccagagtt ggctgagttg tctatgtatt atatgaatct agtttcgtta
     4921 gcacgtttgg agcgaaatcc aacagtcaag acagagattt ctcagaaagg atttggagaa
     4981 agcattccga caggattctt ggtctatcca atcgctcaag cagctgatat cacagctttc
     5041 aaggctaatt atgttcctgt tgggacagat cagaaaccaa tgattgagca aactcgtgaa
     5101 attgttcgtt cttttaacaa tgcatataac tgtgatgtct tggtagagcc ggaaggtatt
     5161 tatccagaaa atgagagagc agggcgtttg cctggtttag atggaaatgc taaaatgtct
     5221 aaatcactaa ataatggtat ttatttagct gatgatgcgg atactttgcg taaaaaagta
     5281 atgagtatgt atacagatcc agatcatatc cgcgttgagg atccaggtaa gattgaggga
     5341 aatatggttt tccattatct agatgttttt ggtcgtccag aagatgctca agaaattgct
     5401 gatatgaaag aacgttatca acgaggtggt cttggtgatg tgaagaccaa gcgttatcta
     5461 cttgaaatat tagaacgtga actgggtcct attcgtgagc gccgtattga atttgctaag
     5521 gatatgggag aagtttataa tatgattcaa aaaggtagtg aaagagcgcg tgaagttgcg
     5581 ggtcaaaccc tatctgaggt aaaaggggca atgggacttc attactttaa ctaagtttat
     5641 tttacaagaa actatcattt ctatctcgaa gaaaagatag tttttttatt tacccctgta
     5701 acatttgaca aatttttata gaatggtatg atagatacaa tataaaaaga gtcaagctca
     5761 aaaagaaaga aaagaggaaa cttcgaatgt ctaattggga cactaaattt ttgaaaaaag
     5821 gttttacctt tgatgatgta ttgcttattc cagctgaaag tcatgtgttg cctaacgatg
     5881 cagatttaac aactaaattg gcagataatt tgactttaaa tatcccaatt attaccgctg
     5941 ccatggacac agttacagag agtcaaatgg ccattgctat tgctcgtgca ggcggtctcg
     6001 gagttatcca taaaaacatg tcaattgctc aacaagcaga cgaggttcgt aaggtaaaac
     6061 gttctgaaaa tggagttatt attgatccgt tcttcttgac gcctgaacat acaattgctg
     6121 aagcagatga gcttatgggt cgttaccgca tcagtggtgt tccagttgtt gaaacacttg
     6181 aaaatcgtaa attggttggt attttgacaa accgagatct tcgttttatt tcagattata
     6241 atcaaccaat ttcaaaccat atgactagtg aaaatcttgt tactgctcct gtgggtacgg
     6301 atcttgcaac ggctgagagt attcttcaag agcatcgtat tgaaaaactt ccgttggtcg
     6361 atgaagaagg cagtctttct ggtttgatca ctatcaaaga tattgaaaaa gttattgagt
     6421 ttccaaatgc ggctaaagat gagtttggtc gtcttctagt tgcaggtgca gtaggtgtta
     6481 cttcagatac atttgaacgt gcagaggctc tttttgaggc aggagcggat gcgattgtta
     6541 ttgatactgc acatggtcat tctgcaggtg tcttgcgtaa aattgccgag attcgtgctc
     6601 atttcccaga tcggactttg attgctggaa atattgctac tgctgaaggt gcacgtgccc
     6661 tttatgaagc gggtgtagac gttgttaagg ttggtattgg accaggttct atctgtacta
     6721 ctcgtgtgat tgctggtgtt ggtgttccgc aagtaacagc tatctacgat gctgcagctg
     6781 ttgcgcgcga atatggtaaa acgattattg ctgacggtgg gatcaagtat tctggagata
     6841 ttgtaaaagc acttgctgca ggtggaaatg ctgttatgct tggatctatg tttgctggaa
     6901 ctgatgaagc tccaggcgaa actgaaatct tccaaggacg taaattcaag acttaccgtg
     6961 gtatgggatc aattgctgct atgaagaaag gttcaagcga ccgttatttc caaggttctg
     7021 tcaatgaagc aaacaagctt gttccagaag gaattgaagg tcgtgttgct tataaaggag
     7081 cggcagctga tattgttttc caaatgattg gtggtattcg ctctggtatg ggttactgtg
     7141 gtgcagctaa ccttaaagaa ctacacgata atgctcaatt tattgaaatg tctggtgctg
     7201 gtttgaaaga aagccatcct catgatgtgc aaattactaa tgaggcacca aattattcta
     7261 tgtaaaaaac aatgaaaaga actccagtga aaacaggagt tcttttacaa tgttgtcaat
     7321 ttccatttac agcagcttta ccatcctgaa tagtgaagat acttagattt tctggcagat
     7381 tttgaagatg gtctaagctt gttgttgtga taaaggtttg gattgattga gaaatcgttt
     7441 ctaataattt taactgtcta gtgttgtcaa gttcactcat cacatcgtca agcagtaata
     7501 taggagattc tgtggtaatg ctttccatta attcgatttc tgctaatttt atcgagagga
     7561 cgagactacg atgttgacct tggcttccga aactagcatc catcccattt atataaaaag
     7621 aaatgtcatc tcgatgagga ccgacaccag tattcttttt aaataaatct ctggatctac
     7681 ttttttctaa agcaattttg aaagattcgg ataagttttg tttgtcagtt atattgacag
     7741 aagattgata ggatattgac aactcttcga tctgattaga gagttcaaaa tgtttcttac
     7801 gcccaaatga ttctagtttt tttatgaaat ctaagcggtg attcattaca cgacatccat
     7861 aatcaactag ctgatcatct aacacagaaa ggaatgtttc atctattttt tgagctgatt
     7921 ttaggtaagt gtttctttgc tttaggatgt ggttataatt ggttaagtca gataaataga
     7981 ttggcttaat ttgcccaagt tccatatcaa tgaattttcg tcgaatcgaa ggtgctcctt
     8041 taattagttg taaatcttca ggagcaaata agacaacatt catgtgtcct acataatctg
     8101 aaaggcgtgc ctgttttaag tgattaactt ttgttacacg gcctttttgt gttagttcga
     8161 tttctagggg aatggatcca gtttttttct gaacgagacc tgaaagatga agttgttcct
     8221 catcaaaatg aatgagattt ttatctgttc gagttcgatg actacgcgtt aaggctaaaa
     8281 aatagatagc ctctaacatg tttgtttttc cttgtgcatt acgtcccaaa aagacattca
     8341 atttaggatt aaagtctatt ttcgtttctt tgtaattacg aaaagtcttg agagatagat
     8401 gttgtagcca catgattatc taccagggaa tcgtggagct tgttggcttt taggtgatga
     8461 agcaggtttc gatttgtctt ttttaactcc cttattcatc tttttgacaa gtttagcgat
     8521 ccgttctttt tcaactttat cagcttggta ttcatcttgt tcttcagaag tagattgtgt
     8581 caacaagatg tcaatgttca tgtcagggat gtcaatttta tcgccaatac gaagtttttt
     8641 accacgacga ctctctaatt ccctattaaa gtaaacagaa tgttcagaga gaaatgattt
     8701 aatagctcct ccgctatgtg taattccaag ttctttgagt agtgcttgga gggtaataaa
     8761 ttcttcaaat aatttgtatt ccataattca cctcaatctt tggtattata ccatattttc
     8821 ctaaaaatag tgagagatac agcgagaaat gtaagcgatt tttatttcaa aagtgttaca
     8881 gggaacaaga aaatttcagg ttttcgtgat ataatagaag tctgtatata aggagataaa
     8941 tcatggagtt agtgcatgga atttcaacac attttatcca atcaaaaaag tttaaaacaa
     9001 acaaaattac cgtgcgtttt accgctccat tatcccttga tacgattgca ggtcacatgt
     9061 tgagtgcaag tatgctagag actgctaatc agatgtaccc cacttctcaa gatttgagga
     9121 gacacttggc cagtctatac ggtacagata tgtcaaccaa ttgtttcaga agagggcaaa
     9181 gccacattat agaattgaca tttacctatg ttcgtgatga gtttttaagt aggaaaaacg
     9241 tgctaacttc tcagattttg gaacttgtaa aagaaactct tttttcaccc gtagtagttg
     9301 ataatgggtt tgatccggcc ttatttgaaa ttgagaaaaa acaattgcta gcaagtttag
     9361 cagctgatat ggatgattct ttttattttg cacataaaga attggataaa ttgttttttc
     9421 atgatgaacg tcttcaattg gaatatagtt atttacgaaa tcgtatttta gctgaaactc
     9481 cacaaagttc ttattcttgt ttccaagaat ttttagccaa tgatcgaata gatttctttt
     9541 tcctaggtga ttttaatgag gttgaaattc aaaatgtatt agaatcattt ggctttaaag
     9601 gtcgaaaagg agatgtgaag gttcagtatt gtcaacctta ttctaatatc cttcaggaag
     9661 gtatggttcg gaaaaatgtg ggacaatcca ttttggaatt aggttatcat tgcccttcta
     9721 aatatggtga tgagaaacat ttacccatga ttgtaatgaa tggtttactt ggtggatttg
     9781 ctcactctaa actttttaca aatgtccgtg aaaatgctgg attagcttat actatttcaa
     9841 gtcagctcga tttatttagt ggattcttga ggatgtatgc tggtattgac cgagaaaatc
     9901 gtaaccaggc tcgtaaaatg atgaataatc aactgcttga tttaaaaaaa ggttatttta
     9961 cagagcttga gttagagcag accaaggaaa tgattcgtcg gtctttgtta ctttctcaag
    10021 ataaccaatc ttcattgatt gaacgtgctt atcaaaatgc cttacttgga aaatcttcag
    10081 cagattttaa aagttggatt gcaaaactcg agcaagttga caaagatgct atttgtagag
    10141 cagctaacaa tgtgaaacta caagcgattt actttatgga aggaatagaa tgacaaaggt
    10201 tgtttttgaa gaaaaatact atccagctgt aaaagaaatg gtttatcgaa ctcgtttggc
    10261 aaatggattg acagtagctc ttttgcctaa aaaggaattt aaagaggttt acgggagtgt
    10321 aactgtacag tttggttcgg tagatacgct tgtcacagaa gttgatggag atgtaaaaca
    10381 atatcctgca ggaattgctc attttcttga acataaatta tttgaaagag aagattctag
    10441 cgatttgatg tcggctttta cgagtctagg tgcagatagt aatgccttta caagctttac
    10501 aaaaacaaac tatctttttt cagcaacgga ttatttttta gaaaatttag atttacttga
    10561 tgaattgata acatcagcac actttactga agcttccatt ctgacagagc aggatattat
    10621 tcagcaagaa cgagaaatgt accaagatga tccagattcg tgtttattct tttcaacttt
    10681 agcgaatttg tatcctggta cacctttagc aactgatata gttggaagtg aggagtccat
    10741 ttcccaaatc aatctaacta atttgcaaga aaattttaca aagttttaca aacctgtaaa
    10801 catgtctctg tttttagttg gtaattttga tgtggagcga gtacaggact attttgaaag
    10861 caaagaactg aaagattcag attttcagga agtagcaaga gaaaagttgt ttttacagcc
    10921 tgtaaagcta acagatagta tgagaatgga agtatcttct cccaaactag cgattggagt
    10981 tagaggtaag cgagaagttt ctgaagcgga ttgctatcga catcatattt tattaaaatt
    11041 attgtttgca atgatgtttg gttggacttc ggatcgtttt caaaaatgtt atgaatcagg
    11101 taaaattgat gcgtccttat ctctggaagt tgaaataaca agtcgctttc attttgtcat
    11161 gttgacaatg gatacgaaag agccagttgc tttgtctcat caatttagga aggctattcg
    11221 taattttaca aaggatttag atattacaga ggaacattta gatattatca aaagagagat
    11281 gtttggcgaa tttttcagta gcatgaactc tcttgaattt attgcaacgc aatatgatgc
    11341 ttttgaaaat ggtgagacaa tttttgattt gccgaaaatt ttacaggaaa ttactttaga
    11401 ggatgtcctt gatgctggac atcatttaat agatgatggt gacatagttg attttacaat
    11461 atttccatcg tagtaaccta tcataataga cactagaaag aagggatgac aagtatgaga
    11521 aaaaaaacaa ttggagaggt tttacgatta gctagaatca atcagggatt gagtttagat
    11581 gaattgcaga aaaagacaga aatccagtta gatatgttgg aagcaatgga agcagacgat
    11641 ttcgatcaac ttccaagtcc tttttacacg cgttctttct tgaaaaaata tgcatgggct
    11701 gttgagttag atgaccaaat tgttttggat gcttatgatt ctgggagtat gattacttat
    11761 gaggaagtag atgttgatga agatgagttg acaggtcgta gacgttcaag taagaaaaag
    11821 aagaaaaaaa catcattttt acctttattt tattttatcc tttttgcttt atcgatttta
    11881 atttttgtga cttattatgt ttggaactat attcaaactc aaccagagga gccttctctt
    11941 tctaattaca gtgtggttca atcaacaagt tcaactagct ctgttcccca ctcctcaagt
    12001 agtagttctt ctagtataga atcagctata agtgtatcag gcgaaggaaa tcatgtagaa
    12061 atcgcttata agacaagtaa ggaaacagtt aaattgcaat tggcagtttc agatgttaca
    12121 agttgggtca gtgtttcaga aagcgaactt gagggcggtg taaccttatc gccaaagaag
    12181 aaaagtgcag aagcaacagt tgcaactaaa agtcctgtaa caattacgtt aggtgttgta
    12241 aaaggtgttg atttgacagt agataatcag actgttgatt tatcgaaatt aacagctcag
    12301 actggacaaa tcactgtaac ctttactaaa aattaaggaa aaacgaatga aaaaagaaca
    12361 aattcccaat ctcttaacaa taggtcgaat tctctttata cctattttta tctttatttt
    12421 aacgatagga aattcgatag agagtcatat agttgcagct attatctttg ctgttgccag
    12481 tattaccgac tatttagatg gatatttagc tcgtaaatgg aatgtggtca gtaattttgg
    12541 taaatttgca gatcctatgg cggataagtt actagttatg tcggctttta ttatgttgat
    12601 tgagttaggt atggctccgg cttggattgt tgcagtgatt atatgtcgtg agttagctgt
    12661 gacaggttta aggcttttat tggttgaaac tggtggaaca attttagcag cagcaatgcc
    12721 tggaaaaatt aaaactttta gtcagatgtt tgctattatt ttcttgctat tacattggac
    12781 tttgcttggt caagttctac tttatgtagc cttatttttc actatctact ctggctatga
    12841 ctattttaag ggtagtgcct atgtatttaa agggacattt ggttcgaaat gaaatcaata
    12901 attgatgtaa aaaatctttc ttttcgctat aaagaaaatc agaactacta cgatgtgaag
    12961 gatattacgt ttcacgtgaa acgtggagaa tggctttcga ttgtagggca taatggtagt
    13021 ggtaaatcaa cgacggttcg attaattgat ggcttactgg aagcagaatc cggagagatt
    13081 gtaattgatg gccaacgttt gactgaggaa aatgtttgga atatacgtcg tcaaatcggt
    13141 atggtttttc aaaatccaga caatcaattt gttggagcga ctgttgaaga tgatgttgct
    13201 tttggtttgg aaaatcaggg actttctcgt caagaaatga aaaagagagt ggaagaagct
    13261 ctggctttag ttggcatgtt ggactttaaa aagagagagc cagcgcgtct atcaggtggt
    13321 caaaagcaac gtgtggccat tgcaggtgtt gtagccctaa gaccagctat tttaatctta
    13381 gatgaagcaa cgagtatgtt ggatcctgag gggcgtagag aacttattgg gacagtaaaa
    13441 ggaattcgaa aagactatga tatgacggtc atttctatta cccatgattt ggaagaagtc
    13501 gccatgagtg atcgcgtatt ggtcatgaaa aaaggggaaa ttgaatcaac tagtagtcca
    13561 agggagcttt tctctcgaaa tgatttagat caaattggat tagacgatcc ttttgctaat
    13621 caattaaaaa aatctttgag ccagaatggc tatgatttac ctgaaaatta tttgacagaa
    13681 agtgagctag aggataagct atgggaattg ctctagaaaa tgtgaatttt acatatcaag
    13741 aaggtactcc cttagcttca gcagctttgt cggatgtttc tttgacgatt gaagatggct
    13801 cttatacagc tttaattggg cacacaggta gtggtaaatc aactatttta caactcttaa
    13861 atggtttatt ggtgccaaat caagggagtg tgagggtttt tgatacctta atcacctcga
    13921 cttctaaaaa taaagatatt cgtcaaatta gaaaacaggt tggcttggta tttcagtttg
    13981 ctgaaaatca gatttttgaa gaaacggttt tgaaggacgt tgcttttgga ccgcaaaatt
    14041 ttggagtttc tgaagaagat gctgtgaaga ctgcgcgtga gaaactggct ctggttggaa
    14101 ttgatgaatc actttttgat cgtagtccgt ttgagctgtc agggggacaa atgagacgtg
    14161 ttgccattgc aggcatactt gccatggagc cagctatatt agtcttagat gagccaacag
    14221 ctggtctaga tcctctaggg agaaaagagt tgatgaccct gttcaaaaaa ctccaccagt
    14281 cagggatgac catcgtcttg gtaacgcatt tgatggatga tgttgctgaa tatgcgaatc
    14341 aagtctatgt aatggaaaag ggacgtttag taaagggggg caaaccaagt gatgtctttc
    14401 aagacgttgt ttttatggaa gaagttcagt tgggagtacc taaaattacg gccttttgta
    14461 aacgattggc tgatagaggc gtgtcattta aacgattacc gattaagata gaggagttca
    14521 aggagtcgct aaatggatag tatgatttta gggcgttata tcccagggga ttcgattgtt
    14581 caccgattgg atccacgtag caaattgctt gctatgatgc tactgatttt gattgtgttt
    14641 tgggctaata accccttgac gaatctgatt ctttttatag cgacagggat atttattgcc
    14701 ttgtcaggag tatctctttc attttttatt cagggcttga agtctatgtt tttcttgatt
    14761 gccttcacaa ctatttttca actatttttc atttctaatg ggaatgtttt atttgagttt
    14821 tcgtttgtga gaatcacgga ttatgctttg caacaagctg ggattatttt ttgtcgcttt
    14881 gtattgatta ttttcttttc aactttgtta accttaacaa ccatgccttt aagtttggca
    14941 tcagctgtcg aagctttatt agcaccttta aagcgtgtga aagttccagt tcatgaaatt
    15001 ggattgatgc tgtctatgag tttgcgtttt gtcccaacct tgatggatga tacgactcgg
    15061 attatgaatg cacagaaagc tcgtggagtg gattttggag aaggaagcat cgttcaaaaa
    15121 gtaaaggcga tgattcccat tttgattcct ctttttgcga caagtttaaa acgtgcagat
    15181 tccttggcta tcgctatgga agcgcgtggc tatcagggtg gaaaaggcag aagtcaatac
    15241 cgacaattga aatggactct aaaggatacg ctgaccattc ttgttattct cgtacttggt
    15301 tgttgtttat ttttcttaaa atcttagtag ctttcaggaa ttgataaaaa gttactgtaa
    15361 cagtttttga tataaaggta ggaatatgaa ccgttttaaa aaatcaaaat atgtcattat
    15421 tgtttttgtc actgttctgc ttgtgtcagc tctcttagcg acgacttatt caagtacaat
    15481 tgtgacaaaa ttaggagatg gaatctcatt ggttgataga gttgtacaaa aaccttttca
    15541 gtggtttgat tctgtcaaat cagatttggc tcatttgaca cgaacatata atgaaaatga
    15601 aagtttgaag aaacagcttt accaattaga agttaaatca aatgaggtgg aaagtttaaa
    15661 gacagaaaat gaacaactgc gccaattgct tgatatgaag tctaaattgc aagccacaaa
    15721 gactttagca gcagatgtta ttatgcgttc tccggtatct tggaagcagg agttgacctt
    15781 agatgcaggt agatcaaaag gtgcttctga gaacatgtta gctattgcaa atggtggctt
    15841 gattgggagt gtttcaaaag tagaggagaa ctctactata gtcaaccttc tgacaaatac
    15901 ggaaaatgct gataagattt ctgttaaaat tcaacatggc tctactacaa tttatggaat
    15961 tattattggc tatgacaagg aaaatgacgt tcttaaagtt agccaattaa atagtaatag
    16021 cgatattagt gcgggagata aggtgactac tggtggatta ggaaacttta acgttgctga
    16081 tattcctgtt ggtgaagtgg ttgccacaac gcatagtaca gactatttga cacgagaagt
    16141 aactgttaaa ttgagtgcag atactcataa tgtagatgtg atagaattag tggggaattc
    16201 ataatgagac agttgaagcg agttggagta tttttattgc ttcctttctt tgttctaatt
    16261 gacgcccata ttagccagct tctgggctca tttttccccc atgtacattt ggctagtcat
    16321 tttctttttc tatttctctt atttgagacg atagaagtat cagagtatct ctacctagtc
    16381 tattgttttg ttataggctt ggtttatgat gtttactttt tccatctaat agggattaca
    16441 actctcttat ttatcttatt gggagccttc cttcataaat tgaatagtgt tattttgttg
    16501 aatcgttgga caagaatgct agctatgatt gtactgacat tcctgtttga aatgggtagt
    16561 tatcttttgg cttttatggt agggttgaca gtagatagca tgtcgatttt tatagtctat
    16621 agcttggtac cgacgatgat tttaaatttt ttatggatta ctgtttttca atttattttt
    16681 gaaaaatatt atctataaga acgacatata aatgtaacaa aggcgtaata tttattaggc
    16741 ctttttttgg tatactagta ttgtctttaa aagaaggagt atctacgtaa tatgaagaaa
    16801 aaaatcttag cgtcactttt attaagtaca gtaatggttt ctcaagtagc tgttttaaca
    16861 actgcgcatg cagaaacgac tgatgacaaa attgctgctc aagataataa aattagtaac
    16921 ttaacagcac aacaacaaga agcccaaaaa caagttgacc aaattcagga gcaagtatca
    16981 gctattcaag ctgagcagtc taacttgcaa gctgaaaatg atagattaca agcagaatct
    17041 aagaaactcg agggtgagat tacagaactt tctaaaaaca ttgtttctcg taaccaatcg
    17101 ttggaaaaac aagctcgtag tgctcaaaca aatggagccg taactagcta tatcaatacc
    17161 attgtaaact caaaatcaat tacagaagct atttcacgtg ttgctgcaat gagtgaaatc
    17221 gtatctgcaa acaacaaaat gttagaacaa caaaaggcag ataaaaaagc tatttctgaa
    17281 aaacaagtag caaataatga tgctatcaat actgtaattg ctaatcaaca aaaattggct
    17341 gatgatgctc aagcattgac tacgaaacag gcagaactaa aagctgctga attaagtctt
    17401 gctgctgaga aagcgacagc tgaaggggaa aaagcaagtc tattagagca aaaagcagca
    17461 gctgaggcag aggctcgtgc agctgcggtg gcagaagcag cttataaaga aaaacgagct
    17521 agccaacaac aatcagtact tgcttcagca aacactaact taacagctca agtgcaagca
    17581 gtatctgaat ctgcagcagc acctgtccgt gcaaaagttc gtccaacata cagtacaaac
    17641 gcttcaagtt atccaattgg agaatgtaca tggggagtaa aaacattggc accttgggct
    17701 ggagactact ggggtaatgg agcacagtgg gctacaagtg cagcagcagc aggtttccgt
    17761 acaggttcaa cacctcaagt tggagcaatt gcatgttgga atgatggtgg atatggtcac
    17821 gtagcggttg ttacagctgt tgaatcaaca acacgtatcc aagtatcaga atcaaattat
    17881 gcaggtaatc gtacaattgg aaatcaccgt ggatggttca atccaacaac aacttctgaa
    17941 ggttttgtta catatattta tgcagattaa tttacagagg gactcgaata gagccctctt
    18001 ttcaggtttt accgtgacaa tccctattaa aaattatatc aaaatagctt gaaaatattg
    18061 gaaaagtatg gtaaaatgaa aattgtcgtg tgaacgataa tactcattct tgatgaattg
    18121 tgaagcagtt gcccttgggt cgttttgcga gttgaagtca agaagaggaa aaaaacaaaa
    18181 aggagaaata ctcatggcag taatttcaat gaaacaactt cttgaggctg gtgtacactt
    18241 tggtcaccaa actcgtcgct ggaatcctaa gatggctaag tacatcttta ctgaacgtaa
    18301 cggaatccat gttatcgact tgcaacaaac tgtaaaatac gctgaccaag catacgactt
    18361 catgcgtgat gcagcagcta acgatgcagt tgtattgttc gttggtacta agaaacaagc
    18421 agctgatgca gttgctgaag aagcagtacg ttcaggtcaa tacttcatca accaccgttg
    18481 gttgggtgga actcttacaa actggggaac aatccaaaaa cgtatcgctc gtttgaaaga
    18541 aattaaacgt atggaagaag atggaacttt cgaagttctt cctaagaaag aagttgcact
    18601 tcttaacaaa caacgtgcgc gtcttgaaaa attcttgggc ggtatcgaag atatgcctcg
    18661 tatcccagat gtgatgtacg tagttgaccc acataaagag caaatcgctg ttaaagaagc
    18721 taaaaaattg ggaatcccag ttgtagcgat ggttgacacc aatactgatc cagatgatat
    18781 cgatgtaatc atcccagcta acgatgacgc tatccgtgct gttaaattga tcacagctaa
    18841 attggctgac gctattatcg aaggacgtca aggtgaggat gcagtagcag ttgaagcaga
    18901 atttgcagct tcagaaactc aagcagattc aattgaagaa atcgttgaag ttgtagaagg
    18961 cgacaacgct taatttatac aaatagtaat tacctaggag ggcggggctt agcccggctc
    19021 tcctattttc aaaaaatata ggagaattaa aatggcagaa attacagcta aacttgtaaa
    19081 agagttgcgt gaaaaatctg gtgccggtgt tatggacgct aaaaaagcgc ttgtagaaac
    19141 agacggtgac atcgaaaaag cgattgaatt gcttcgtgaa aaaggtatgg ctaaggcagc
    19201 taagaaagct gaccgtgttg ctgcagaagg tttgactggt gtttatgtta acggtaatgt
    19261 tgcagcagtt attgaagtaa acgctgaaac tgacttcgtt gcaaaaaacg ctcaattcgt
    19321 tgaattggta aatactacag ctaaagtcat tgctgaagga aaacctgcta acaacgaaga
    19381 agctcttgct ttgataatgc cttcaggtga aactcttgaa gctgcatacg tatctgcaac
    19441 agcaactatc ggagagaaaa tctcattccg tcgctttgca ttgattgaaa aaacagacgc
    19501 acaacacttt ggagcatacc aacataacgg tggacgtatc ggtgttattt cagttgttga
    19561 aggtggagac gaagcacttg ctaaacaatt gtcaatgcac atcgcagcga tgaaaccaac
    19621 agttctttct tacaaagaat tggatgagca attcgttaaa gatgagttgg cacaattgaa
    19681 tcacgttatc gaccaagaca acgaaagccg tgcaatggtt aataaaccag ctcttccaca
    19741 cttgaagtat ggatcaaaag ctcaattaac tgatgatgtt attgctcaag ctgaagctga
    19801 catcaaagct gaattggctg cagaaggcaa accagaaaaa atctgggaca aaattattcc
    19861 aggtaaaatg gatcgcttca tgcttgataa cactaaagtt gaccaagctt acacacttct
    19921 tgcacaagtt tacatcatgg atgacagcaa gacagttgaa gcataccttg aatcagttaa
    19981 cgcttcggta gttgagtttg ctcgctttga agttggtgaa ggtatcgaga aagctgcaaa
    20041 cgacttcgaa gctgaagttg cagctacaat ggcagcagcc ttgaataact aataatagaa
    20101 aggaagcaaa ttgcttcctt ttttgtatag ggcttagatt tttcctgaaa ttgttttttc
    20161 cccagtctca ctcgattaga ataattaaaa caaaaagccc tataataagg gctaagtgca
    20221 tttaggagac tatacttcaa attcatagta agcgcgttca taacaaggta tctatcctaa
    20281 ttccccaact tccagtatac tagataaaaa ataattatcg gagtaaaggt attgtactat
    20341 gataattcaa ctaagtgatt taggtcaagt tcaccttgtt tgtggcaaga cagatatgag
    20401 gtagggaata gactcattag cctatgtagt taaaacccac tttgaattgg attctttctc
    20461 cggtcaagtc tttctctttt gtggtg
//
LOCUS       DAVBBS010000041        20158 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVBBS010000041 DAVBBS010000000
VERSION     DAVBBS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20158
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.041"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            63..527
                     /locus_tag="VQU32_001429"
     CDS             63..527
                     /locus_tag="VQU32_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HET2472869.1"
                     /translation="MGGHPGFNCLLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            685..1110
                     /gene="lacA"
                     /locus_tag="VQU32_001430"
     CDS             685..1110
                     /gene="lacA"
                     /locus_tag="VQU32_001430"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HET2472870.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            1141..1656
                     /gene="lacB"
                     /locus_tag="VQU32_001431"
     CDS             1141..1656
                     /gene="lacB"
                     /locus_tag="VQU32_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HET2472871.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            1667..2596
                     /locus_tag="VQU32_001432"
     CDS             1667..2596
                     /locus_tag="VQU32_001432"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HET2472872.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDDQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKLVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLEELKEVLQESLFTGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAGLLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            2598..3578
                     /gene="lacD"
                     /locus_tag="VQU32_001433"
     CDS             2598..3578
                     /gene="lacD"
                     /locus_tag="VQU32_001433"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HET2472873.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKILVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            3812..4648
                     /locus_tag="VQU32_001434"
     CDS             3812..4648
                     /locus_tag="VQU32_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET2472874.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENH
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALIQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            4684..5001
                     /locus_tag="VQU32_001435"
     CDS             4684..5001
                     /locus_tag="VQU32_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2472875.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            5003..6697
                     /locus_tag="VQU32_001436"
     CDS             5003..6697
                     /locus_tag="VQU32_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2472876.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
                     VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFS
                     ATDGYIGLAIVFGAYAFFWFVGIHGPSVVEPLIVAISYANIEANVQLVQAGMHADKIL
                     NPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSF
                     VLAITLIIIDTLIYYPFVKVYDHQILEEERSGKSNDELKEKVAANFNTAKADAILEKA
                     GVDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLP
                     EFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQAQFD"
     gene            6906..8312
                     /gene="lacG"
                     /locus_tag="VQU32_001437"
     CDS             6906..8312
                     /gene="lacG"
                     /locus_tag="VQU32_001437"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET2472877.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPLDPKNPSDVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            8355..8537
                     /locus_tag="VQU32_001438"
     CDS             8355..8537
                     /locus_tag="VQU32_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HET2472878.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQIPCLTSEELKSLFLNFINPEE
                     WDTISFDSDFMPFQQS"
     gene            complement(8815..9576)
                     /locus_tag="VQU32_001439"
     CDS             complement(8815..9576)
                     /locus_tag="VQU32_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2472879.1"
                     /translation="MLKQEKLAKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNVSNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(9758..10720)
                     /gene="nrdF"
                     /locus_tag="VQU32_001440"
     CDS             complement(9758..10720)
                     /gene="nrdF"
                     /locus_tag="VQU32_001440"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HET2472880.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(10908..13067)
                     /gene="nrdE"
                     /locus_tag="VQU32_001441"
     CDS             complement(10908..13067)
                     /gene="nrdE"
                     /locus_tag="VQU32_001441"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HET2472881.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(13148..13366)
                     /gene="nrdH"
                     /locus_tag="VQU32_001442"
     CDS             complement(13148..13366)
                     /gene="nrdH"
                     /locus_tag="VQU32_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HET2472882.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            13717..13980
                     /locus_tag="VQU32_001443"
     CDS             13717..13980
                     /locus_tag="VQU32_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HET2472883.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            13986..15719
                     /gene="ptsP"
                     /locus_tag="VQU32_001444"
     CDS             13986..15719
                     /gene="ptsP"
                     /locus_tag="VQU32_001444"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET2472884.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            15974..18424
                     /gene="phtA"
                     /locus_tag="VQU32_001445"
     CDS             15974..18424
                     /gene="phtA"
                     /locus_tag="VQU32_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HET2472885.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHF
                     IPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSN
                     SSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKE
                     NVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDL
                     LAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYV
                     TPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVK
                     GEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLF
                     ATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVP
                     QVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKL
                     LALLKGSNPSSVSKEKIN"
     gene            18582..>20158
                     /locus_tag="VQU32_001446"
     CDS             18582..>20158
                     /locus_tag="VQU32_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2472886.1"
                     /translation="MKINKKYLAGSVATLVLSVCAYELGLHQAQTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYQLKDEDIISEIKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTSTNDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWLPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGNHYHF
                     IPYEQMSELEKRIARIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSLQPAPNPQPAPS
                     NPIDEKLVKEAVRKVGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLG
                     AKKTDLPSSDREFYNKTYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKL
                     VDDILAFLAPIRHPERLG"
BASE COUNT         6437 a         3612 c         4159 g         5950 t
ORIGIN      
        1 aactagaatt ctagttttgc aggaagtcta ataaagaaac tagcaaggtc aggccttact
       61 ttattggtgg acatccagga tttaattgtc ttctacttga tgatgaagtc tatgaagatt
      121 attatctaga gtttgagaaa gaagagattt gctctgttcc acgtcctttc ccagaaacag
      181 gtatgttgga tttccaagat agaagtccat ggctagaggg acaaaaagaa atagatctta
      241 gttatgatct tttcagcaca gatgcagtga ctttggatga attgcaatct cgaacaattg
      301 cccttcgttc tcttaaacat gataagggat tgaaagtgca ttttgcagaa tttccaaacc
      361 tcatcatctg gtcaactttg aacaagggac ctttcattac ctttgaacca tggtctggct
      421 tgtcaacatt ccttgaagaa ggagatcatt tagaagataa gaagaatgtt tgtctcttag
      481 aagccaatca ggttgaagaa ttagggtttg agatagaagt tttataaaaa aagtaaaaaa
      541 taaataacaa aaacaaacac aaactgttga cttcttcaaa caatagtagt atattatgtt
      601 tgtgaggact aattgactgt tttttaaaga cagccaatac actgctggaa tccagcatag
      661 aaaaaataaa aaggagtata caatatgtct attgttatcg gtgcagatgc tgcaggtttg
      721 agattgaaag aagttgtgaa agacttctta gaaaaagaaa acttccacct tgtggatgtt
      781 acagctgaag gtcaagactt tgttgatgtg actctcgctg ttgctgcaga agtaaacaaa
      841 gaagaacaaa accttggtat cgtgattgat gcttatggag ctggtccatt tatggttgca
      901 actaagatca aaggaatggt tgctgcagaa gtatctgacg aacgttcagc ttatatgact
      961 cgtggccaca acaactcacg tatgatcact atgggggcac aacttgttgg tgatgaattg
     1021 gctaaaaaca tcgctaaagg atttgttaat ggtaaatacg acggtggacg tcatcaaatc
     1081 cgggttgaca tgttgaacaa aatgggctaa ttagattaca gtaagaaagg taagttaaaa
     1141 atgagaattg caattggatg cgaccacatc gtaacagatg aaaaaatggc ggtttcagaa
     1201 tttttgaaat caaaaggata tgaagtcatt gactttggta catatgacca tacacggact
     1261 cactacccaa tctttggtaa aaaagtaggg gaagctgtaa ctagcggcca agctgatctt
     1321 ggggtatgta tctgtggtac tggtgttggt atcaacaacg ctgtaaataa agttccaggt
     1381 gttcgttctg ccttggttcg tgatatgaca acagcccttt atgctaaaga acaattgaac
     1441 gctaacgtta tcggctttgg tggtaagatt actggtgaat tgcttatgtg tgatatcatc
     1501 gaagctttca ttcatgctga atacaaacca tctgaagaaa acaagaaatt gattgcgaaa
     1561 attgaacacc ttgaaagtca caatgctcaa caaacagacg caaacttctt tacagaattc
     1621 cttgaaaaat gggatcgcgg agaataccac gactaagagg tgacctatga ttttaacagt
     1681 cacaatgaac ccatccattg atatttccta tcccttggat gagttgaaga ttgatactgt
     1741 caatcgtgtg gtggatgtaa ccaaaacggc tggtggtaag ggacttaatg ttacccgagt
     1801 actttcagaa tttggtgatt ctgttcttgc tactggttta gtgggtggca aacttggtga
     1861 gtttttggtt gaacatatcg atgatcaagt aaagaaagat ttcttttcta ttcagggaga
     1921 aactcgtaac tgtatcgcta ttctccacgg tgacaaccaa acagaagttc ttgaaaaagg
     1981 tcctgaagta ttggaacagg aaggtcaaga ctttttggaa catttcaaaa aactcttgga
     2041 gtcagttgaa gtagtagcca tctcaggtag tctgccagct ggccttccag ttgattacta
     2101 tgcgagcttg gtagagcttg ctaatcaagc tggcaagcta gtagtcttgg actgctctgg
     2161 tgcagcactt caggcggttc ttgaatcacc ccataaaccg acagtaatca aacccaataa
     2221 tgaggaattg tctcagcttc taggaagaga agtttctgag gatttggagg aattaaaaga
     2281 agtccttcaa gaatctttgt ttacagggat tgaatggatt atcgtttcac ttggtgccaa
     2341 cggtactttt gccaaacatg gtgacacttt ctacaaggta gatattccta gaattcaggt
     2401 ggtaaatcct gttggatctg gagactctac tgtggcagga atttcttcag gacttcttca
     2461 caaagaatcg gatgcaggat tactcatcaa ggcaaatgtc cttggtatgc tcaatgctca
     2521 agaaaaaatg actggtcatg tcaacatggc caactatcaa gctctatatg atcaattaat
     2581 agtaaaagag gtataaaatg gctttaacag aacaaaaacg tgtacgctta gaaaaacttt
     2641 ctgatgaaaa tggtatcatc tcagctcttg catttgacca acgtggtgct ttgaaacgcc
     2701 tcatggctca acaccaaaca gaagaaccaa ctgtggccca aatggaagaa cttaaaatct
     2761 tggtagcaga tgaattgact aaatacgctt catctatgct tcttgaccct gagtatggac
     2821 ttccagcaac taaggctctt gatgaaaaag ctggtcttct ccttgcttat gaaaaaacag
     2881 gttatgacac aacaagcaca aaacgcttgc cagactgctt ggatgtttgg tctgcaaaac
     2941 gtattaaaga agaaggtgca gatgcagtta aattcttgct ttactatgat gtagatagct
     3001 cagacgaact caatcaagaa aaacaagcct acatcgaacg catcggttct gagtgtgtgg
     3061 ctgaagatat cccattcttc cttgaaatcc ttgcttacga tgaaaaaatt gcggacgcag
     3121 gttctgtaga atacgctaaa gtaaaaccac acaaagttat cggtgctatg aaagtctttt
     3181 cagacccacg ttttaacatt gatgtcttga aagttgaagt tcctgttaac attaaatatg
     3241 ttgaaggctt cgctgagggt gaagtggttt acacacgtga agaagcagca gccttcttca
     3301 aagcgcaaga tgaagcaacg aacttgccat acatctactt gagtgctggt gtatcagcta
     3361 aactcttcca agatactctt gtatttgctc atgaatcagg tgcaaacttt aacggagttc
     3421 tttgtggtcg tgctacatgg gcagggtcag ttgaagctta catcaaagat ggtgaagcag
     3481 cagctcgcga atggcttcgc acaactggat ttgaaaacat tgatgagctc aataaagttc
     3541 ttcaaacaac agcgacttca tggaaagaac gtgtgtaaga aagtcctcct agtttaggaa
     3601 catgaatcta aaaaaattta aaaaaagttg tatgtaaagg tttacaaaat aacttacttg
     3661 tgctatactt aaatcacaag ttaatacaag gtgagtgtta ctaagtaata ttaggcatga
     3721 tcacaggtga attagaaatc agctgatttt ctagttcatt tgtggtcatt ttttgtactc
     3781 atataccttt aagatataaa aggaggttga catgtatcga attctaaatc caatgaatca
     3841 caatgtctcg cttgtcagaa atgataaggg agaagaggtg attgtaattg gtaaggggat
     3901 tgcattcgga aagaagaagg gggatttgat tgctgaaaat catgttgaga aaatctttcg
     3961 gatgaagacc gaagagtcca gagaaaactt tatggctctt ctcaaagatg ttccgcttga
     4021 ttttatcaca gtgacctatg aaatcattga taagctatca aagaaatatc attatccgat
     4081 tcaagagtat ctctatgtaa ccttgacaga tcatatttac tgttcttatc aagctctaat
     4141 tcaaggaagg tacaaggata gtaatctgcc agatatttct gctaagtatc ctgtcgcttt
     4201 tcaaatcgca aatgaagctt ttgaaattta ccgtcagaag ctagcagatc attttcctga
     4261 ggacgaaatt attcggattg cttatcattt cattaatgct gaaggtgaaa atgaagtgga
     4321 acttgtggag tcgattgata agaggaaaga aattctcagg aatgttgaag aagttttaac
     4381 ggactatgca attcaacgaa ctaaaaagaa taaccatttc tatgatcgct ttatgatcca
     4441 tttgaattat ttcttggatt atttagacag atctagagat gataaccaat cacttctgga
     4501 tatggaagat catattaaac aatcctatcc aaaagccttc gagattggtt ccaagatcta
     4561 tgatgtgatt acgcaacata cgggtcttga tttgtataaa agtgaacgag tttatctagt
     4621 tctacatatc caacgtttat tgtcataaaa atttatttaa aactatataa ggagaattct
     4681 atcatgaata gagaagaagt aacattgtta ggttttgaaa tcgtagccta tgctggcgat
     4741 gctcgttcaa aactattgga agccttgaag gctgctgaag ctggtgattt tgaaaaagcg
     4801 gacgctctgg tagaggaagc tggtagctgt attgcagagg ctcaccacgc gcaaacaagt
     4861 ctattgacta aggaagcttc tggtgaggac ttagcgtata gtgtgaccat gatgcatggt
     4921 caagatcact tgatgacaac tatcttgtta aaagacctaa tgcaccattt aattgaactt
     4981 tacaagagag gagttaaata aaatggataa gttaataatt tttattgaga aaggaaagcc
     5041 tttctttgag aaattatcga gaaatatata cttaagagcg ataaaagatg gttttatttc
     5101 cagtatgcca gcagtattat tctcaagtat ttttatacta atagctgctg tgcccaatat
     5161 ttttgggttt aaatggtctg atgaacagtt agcttttatt ttgaaacctt acaactactc
     5221 aatggggatt ttagctctct tagtagctgg gacaactgca aaatcgttaa cagattcagt
     5281 taatacacgt agtatggaga aaaccaacca aatcaactac atgtcaactt ttttagcagc
     5341 tgttgttgga ttgttaatac tggcagctga tccaattgaa ggtggatttg ctaatggtct
     5401 tttaggtact agaggattac ttactgcatt tttggcagct tttattacag taaatatcta
     5461 taaagtatgt attaaaaata atgtaaccat tagacttcct gaagaggttc caccaaatat
     5521 tgcacaagtt tttaaagatg ttattccgtt tgccttatct gttctttcaa tatatggatt
     5581 ggatttaatt gttagaaata tttttggtac aaatgtagca gaatctgttg gtaaaatatt
     5641 agcccctcta ttttcagcta ctgatggtta tatcggttta gctattgtat ttggtgctta
     5701 tgctttcttt tggtttgttg gaattcatgg gccatctgtt gtagaacctt taattgtagc
     5761 tatcagttat gcaaatatag aagctaatgt tcagcttgtg caagctggta tgcacgcaga
     5821 taaaatttta aatccagtta ctcaaacttt tgttgtaaca atgggtggta ctggtgcaac
     5881 actagtagtt ccatttatgt ttatgtggtt atgtaaatca aaaagaaata gaattgtcgg
     5941 acgtgcgtct gtagttccta ctttctttgg cgtaaatgaa ccaatcctat ttggtgctcc
     6001 aattgtttta aatcctatct tctttattcc gtttgttact gctcctatta tcaatgtttg
     6061 gattatgaaa ttttttgtag atgttctaca aatgaatagt ttcagtatta ttttaccttg
     6121 gacaacacct gctccaattg gtattgttat gggaacggcg ctggctccat tgtcattcgt
     6181 cttggctata actttaatca ttatcgacac tttaatctat tatccatttg ttaaagttta
     6241 tgaccatcaa attttagaag aagaacgttc aggtaagtct aatgatgaat tgaaagaaaa
     6301 agttgctgca aacttcaaca ctgcaaaagc ggatgctatt cttgaaaaag cgggtgtcga
     6361 tgcagcacaa aataccatca ctgaagaaac aaatgtcctc gttctctgtg caggtggagg
     6421 aacaagtggt ctccttgcaa atgctttgaa taaggcagca gcagaataca atgtccctgt
     6481 gaaagcagca gcaggcggct atggtgctca ccgtgaaatg ttaccagagt ttgatcttgt
     6541 tatccttgcc cctcaagttg cttcaaactt tgaagatatg aaagcagaaa cagataagct
     6601 cggtattaaa ctagcgaaaa cagaaggcgc tcaatacatc aaattaactc gtgatggaaa
     6661 aggtgctctt gcattcgtac aagcgcaatt cgattaaggc tagagactct gaaatagtct
     6721 cctatcgtta cggaaatcgc tatggcgaat ttcctattat taattcgtcg gtaaaaagat
     6781 atcgttttta cctcctcatg tcacaattcg gtgacttggt acaagaagtg agatggagaa
     6841 ggatggctca ctgactcctc tcctctcact tttactttat ttaaatcaag aaataggtga
     6901 aaaaaatgac aaaaacactt ccaaaagact ttatttttgg tggcgcaaca gctgcctatc
     6961 aagcagaagg tgctacacat actgatggta aaggaccagt agcgtgggat aaatatcttg
     7021 aagataacta ctggtacact gcagaaccag ccagtgattt ttacaatcga tatccagttg
     7081 acctcaagct agcagaagag tatggtgtca atggtattcg aatttctatt gcttggtcac
     7141 gtattttccc gactggttac ggccaagtaa atgctaaagg tgttgagttt tatcataatt
     7201 tatttgcaga gtgtcacaaa cgtcatgttg agccctttgt aactcttcat cactttgaca
     7261 cgccagaagc tctccactca aatggagact tcttaaaccg tgaaaatatc gaacattttg
     7321 tagactacgc tgccttctgt tttgaagaat ttccagaagt aaactattgg acaaccttta
     7381 atgaaattgg gccaattggt gatggtcaat acttggttgg gaaattccct ccaggtattc
     7441 agtacgacct tgccaaagtc ttccaatcgc accacaatat gatggtgtct catgcgcgtg
     7501 cagttaagct atataaagat aaaggctata aaggcgaaat tggtgttgtt cacgctctgc
     7561 caactaagta tcctttagat cctaaaaatc cgtcagatgt tcgtgcagct gagttggaag
     7621 atattattca caataaattt atcttggatg caacttatct aggtcgctat tcagctgaaa
     7681 cgatggaagg tgtcaaccat atcttatcag tcaatggtgg tagcttggat cttcgtgaag
     7741 aagattttac agcattagaa gcagcaaaag acttgaatga tttccttgga atcaactact
     7801 atatgagtga ctggatggaa gcctttgatg gagaaactga aattatccat aatggtaagg
     7861 gtgagaaagg aagttctaaa taccaaatca aaggtgttgg tcgtcgtgta gctcctgatt
     7921 atgtaccacg tacggattgg gattggatta tctaccctca aggtttatat gaccaaatta
     7981 tgcgtgtgaa gaaagattat cctaactaca agaagattta catcactgaa aatggtctcg
     8041 gctataaaga tgagttcgtc gataacactg tttatgatga tggacgtatt gattatgtga
     8101 aacaacactt ggaggtcttg tctgatgcga ttgcagatgg agcgaatgta aaaggttact
     8161 tcatttggtc attgatggac gtcttttcat ggtcaaatgg ttatgaaaaa cgttacggtc
     8221 tcttctatgt agactttgaa actcaagaac gttatccgaa gaaatcagct cactggtaca
     8281 agaaagtagc ggaaactcag attatagact agtagaatta gtcattagat atagaatttt
     8341 agtgagtcaa aaagatgttc aaagatttta tccaatctat ttatgaaaaa gtttatatta
     8401 taaatttcga aaaatgctct caaataccgt gtttgacgag tgaagaattg aaaagtctat
     8461 ttttaaattt tatcaatcct gaagaatggg atactatctc ctttgattca gattttatgc
     8521 catttcaaca atcgtaacca atttctcaaa aaagttaaat cttatattta gtactctgca
     8581 aaactcttat ctaatcacgt tgcttatact caatgaaaat caaagagcaa ctttaaacta
     8641 ggaagcgagt cgcagatttc tcaatgcata gctttgagga attgggcaaa aagtctttga
     8701 tatagaaaaa gcatagtatc aggtgtttca acacctgata ctatgctttt tattgtggga
     8761 agatttactt tttttcttct gaaattgagt tgttacccag gctctttcag tttattaagg
     8821 cttgatgact ttaatgtgtt tagatagctt aaaaaggatt gaatcactta gtttagaatc
     8881 tgaaacaata gtatcaagat ttgatacatt ataaaaagta taaaaatcaa acttattgaa
     8941 cttactatga tctgcgagta aatatttttt attagaatta tttaaagcga tgcgttgagc
     9001 ctctccctct tcctcgctaa aagtagctag agctccgttt tgaataccat tacagctaac
     9061 gaaagcttta gaaaattgga gattagagag attttgtagg gtcaatgtgc caacaaaagc
     9121 acctgtaata tcgcgataat ttccacctat caaaatcaaa tctgttaatt ttcgttcgct
     9181 taaaatcaga aaaacaggta gactgttggt tacgacgcgg atattgtcaa taggcaactc
     9241 acgcgcaaaa aactctaatg ttgttcctgg tccaatgaaa atagtttctc tttcttctac
     9301 tagactgcct gcaaaatggg ctatttcttg tttttctgcc gtttggaggg cttgtttttc
     9361 aatatttgat cgctcattag tcaaaaggga gttggttcga agtttttcag ctccaccatg
     9421 cacacgaatc agcaaatctt tatcagctaa ttcctgtaaa tagcgccttg cagtcatatc
     9481 tgaaacggct atttcgtcca taatctgttt aactgttatg gttcctttac tatttactat
     9541 ctctaaaatt ttggctaatt tttcttgttt gagcatatta tcacctcgtt tcctactact
     9601 atcttaccat aaacaaactc atcattcaaa tacaaaaaca acaaaatgaa acaaaaacaa
     9661 aaatatcgaa gtttgttttc aaaactttcg atatttttgt tgggttataa ctttgatgtt
     9721 tctagtttac tttttgatga ttgagagtga tggagaatta gtctaaaccg tagttatagt
     9781 catcgtcttg catggcttca acttcgccaa gaaggtaacc atttccgact tgagagaaga
     9841 agtcatggtt ggaagttcct gttgaaatac cgttcataac gattgggttg acatcttcag
     9901 ctgaatctgg gaaaagtgga tcttgtccca tgttcatgag agctttattg gcattgtagc
     9961 gaaggaaggt tttaacctct tcagtccaac caacaccgtc ataaagactc tctgtgtagc
    10021 cttcttcatt ttcataaaga gtatagagta ggtcgtacat ccattctttg agtttttctt
    10081 gctcttcttc aggcaattca ttgaaaccaa gttggaattt gtaaccaatg taggttccgt
    10141 gaacagactc gtcacgaata atcaatttaa tgatttctgc aacgttggct agtttgttgt
    10201 taccgagata gtagagggga gtgaagaaac cagagtagaa gaggaaggtt tcgaggaaga
    10261 cgctggcaac tttcttttca agtgggctgc cgtttaggta gatttcgttg acaatctcag
    10321 ccttcttttg taggtaagga ttggtattgg tccattcgaa aatttcttca atctcagcct
    10381 tagtattcaa ggtagaaaag attgatgagt aagatttagc gtggacagat tccataaatt
    10441 ggatgttatt gaaaacagct tcctcatgtg gtgtacggat gtctgcgcga agggcttgaa
    10501 ccccagtttc agattgcata gtgtcaagaa gggttaaacc accaaaaact tttccgacca
    10561 agtctttctc tttgttagat agctttctcc agtcatccaa gtcgtttgat aagggaatac
    10621 gtgtatcgag ccaaaattgc tccgtcagtt tttcccaagt tgatttgtcg atgacatctt
    10681 cgatggcatt ccagttaatg gctttgtagt aagtttccat ttaaaatctc tttctgtgtt
    10741 tagtattgcg aactcacaat tatttctact ttaccataat tctataggag tatcgcacaa
    10801 aaagtcggaa gcccgacttt taaaatgtta cataaattat gttatgacat agtagatttg
    10861 attttatcag tgctgcttag ggaaaaataa tgtttctatg ctagaaacta aatcacacag
    10921 ctttcacatt ggttggcgcc gacttctcca ccgtcatctg taaaggtacg gacgtagtag
    10981 atagacttga ttcccttgtt aaaggcatag ttacgaagga tggacaagtc acgtgtcgtt
    11041 tgtttatttt ccctcttcca ttcgtaaagg ccttttggaa tgtcactacg catgaagagg
    11101 gtgagtgaaa gtccttgatc cacgtgttca gtcgcagcag cgtaaacatc gatgacttta
    11161 cgcatatcca tatcgtaggc agaagtgtag taaggaatgg tttctgtaga caagccagca
    11221 gcagggtagt agattttacc aattttcttc tcttggcgtt cttcgatacg ttgcgtaatc
    11281 gggtggatag aagcagaaac gtcgttgata tagctgatag aaccatttgg cgctacagca
    11341 aggcgatttt ggtggtaaag accatcttct tgaaccttgt cgcgaagttc agcccaatca
    11401 gcaacaccag ggataaagac atttttgaag agttctttaa cacggtctga tgttggaaca
    11461 aattcaccag ttacatactt gtcaaagtaa cttccgttag catagtctga tttttcaaag
    11521 ttgtggaagg taataccacg ttcacgcgca atattgtttg actctaccaa ggtccagtag
    11581 ttcataagca taaagtagat gcttgtaaat tcaacagact caggtgaacc atattcaatg
    11641 agttgttggg caaggtagct gtgcagtccc atggcaccga gaccaaaggt gtgggcttgg
    11701 ctatttccat ggtcaatcgt tggtacagct acgatatgtg aactatctgt aacgaaagta
    11761 agggcacgaa ccatagcacg gatagaacga ccaaaatcag gtgaagtcat catgttaacc
    11821 acgttggttg aacccaggtt acatgaaaca tctgttccca tttgaaggaa ttcttgagca
    11881 tcgttgatca agcttggttc ttgaacttga agaatctcag aacacaagtt actcatgata
    11941 atctttccat caacaggatt tgcacggtta gccgtatcga tgttgactac ataaggatag
    12001 ccagactctt gttgcaattt agagatttca gtttccaaat cccgcgcctt gatttttgtc
    12061 ttgcgaatat ttggatttgc gaccaattca tcgtattttt cagtaatgtc gatgtaattg
    12121 aatggcacac cgtattcttt ttctacagag taagggctga agaggtacat ttcttcattt
    12181 ttacgagcca attcgtagaa tttatcaggt actacaacac caagtgatag agtcttgaca
    12241 cgtacttttt catcagcgtt ttctttctta gttgaaagga aagcgatgat atctgggtga
    12301 aagacgttga ggtagacaac accggcacct tgacgttgcc ccaattggtt ggagtaagag
    12361 aagctgtctt caaaaagctt cataacagga acgacacctg aagcagctcc ttcatagcct
    12421 ttgataggtg caccagcttc acgaaggttg ctgagggtaa ttcccacacc accaccaata
    12481 cgtgaaagtt gaagagctga gttgatagaa cgtccgatag agttcatatc atccgtcact
    12541 tggattagga aacaagatac caactctcca cggcgagcac gtccagcatt caagaaggaa
    12601 ggagtagcag gttggtagcg ttggtggatg atttcattgg caatatcgat tgcaacagct
    12661 tcattcccat cagcgaaata aagggcgttg aagaagacac ggtcttccat attttcaaga
    12721 tagtattcac cgtcattagt ctttaaggca tattgattgt aaaatttata agctgccatg
    12781 aatgacttga attggaagtt ttggtctttg ataaattgag ctaattcttc caagaactct
    12841 ggacggtatt tcttgataaa ggctgtttcg atgtagttgt gttcaatgag gtaattgatt
    12901 ttgtctttga ttgaatcaaa aaccatagtg tttggaacta cattttcttt aaagaaagca
    12961 tccaaggctt ccttgtcttt atgaagcatg atttgtccat taacaggacg gttaatttcg
    13021 ttattaagac ggaagtaagt cacgtcttca agatgtttta atcccataaa atttccctta
    13081 tctaattaca aaagaaaggc ttctaagtta gccctaaaag cagtttcttc tggatgatgt
    13141 actaagatta tgctaattgt ttcagttttc ctggttggaa acctgaaaag acttcagttg
    13201 gtgtttggat aacaggagct gcgctaaaac cgagctcttt aacttgatcg acgtactcag
    13261 gttgctcatc aagattgatt tcacgataag agacattatt actgtccaag aaacgcttgg
    13321 tcattttaca ttggacacaa ttgtttttag aataaacggt taccattgtg taactcctct
    13381 tcaaaattta atactatctt agtatatcag aaaataaaat tttgtcaatg atttgaacta
    13441 aatatagtgg tgttcttttg ttgtttttat atataaacac aatatatagt gtttgtattg
    13501 tgaaatagca atgattatcc tataaattgt ttttaaagaa actatatcct gtgttttgtt
    13561 atctaggata gaagattttc agcaagttat ctgaaaggaa attgaaggaa aatccagaaa
    13621 atgcttgaaa aaaatcctag aagatggtat aatactaaat tgtaagggtt atcacatata
    13681 actcaaaaaa agaaagaaca aaaggagagt caaactatgg cttctaaaga tttccacgta
    13741 gtggcagaaa caggtattca cgcacgtcca gcaacattgt tggtacaaac tgctagcaaa
    13801 tttgcttcag atatcactct tgagtacaaa ggtaaatcag ttaaccttaa atcaattatg
    13861 ggtgttatga gtcttggtgt tggccaaggt gctgacgtaa ctatctcagc tgaaggtgca
    13921 gatgcagatg acgctatcgc tgcaatctca gaaacaatgg aaaaagaagg attggcataa
    13981 gggaaatgac agaaatgctt aaaggaatcg cagcatctga cggtgttgca gttgcaaaag
    14041 catatctact cgttcagccg gatttgtcat ttgagactat tacagtcgaa gatacaaacg
    14101 cagaagaagc tcgccttgat gccgctctac aggcatcaca agacgagctt tctgttattc
    14161 gcgagaaagc agtaggtacg ctcggtgaag aagcagctca agtttttgat gctcacttaa
    14221 tggttcttgc tgacccagaa atgatcagcc aaatcaagga aactatccgt gcgaagaaag
    14281 tgaatgcaga agcaggtctg aaagaagtta cagatatgtt tatcactatc tttgaaggca
    14341 tggaagacaa cccatacatg caagaacgcg cagcggatat ccgcgacgtg acaaaacgtg
    14401 tattggcaaa ccttcttggt aaaaaattgc caaacccagc ttctattaat gaagaagtga
    14461 ttgtgattgc gcatgacttg acaccatctg atacagctca attggacaaa aactttgtaa
    14521 aagcttttgt aaccaacatt ggtggacgta caagccactc agctatcatg gcacgtacac
    14581 ttgaaattgc tgctgtatta ggtacaaata acatcactga aatcgttaaa gacggtgaca
    14641 tccttgctgt taacgggatc actggagaag tgattatcaa cccaacagat gaacaagcgg
    14701 cagaatttaa agcagctggt gaagcctatg cgaaacaaaa agctgaatgg gcacttttga
    14761 aagatgctca aacagtgact gctgacggta aacacttcga gttggctgcc aatattggta
    14821 ctccaaaaga cgttgaaggt gttaacaaca acggtgcaga agctgttgga ctttaccgta
    14881 cagagttctt gtacatggat tctcaagact tcccaactga agatgagcag tatgaagcat
    14941 acaaggctgt tcttgaagga atgaacggta aacctgttgt cgttcgtaca atggatatcg
    15001 gtggagataa ggaacttcct tacttcgata tgcctcacga aatgaaccca ttccttggat
    15061 tccgtgctct tcgtatctct atctctgaga ctggagatgc tatgttccgc acacaaatcc
    15121 gtgctcttct tcgtgcgtct gttcacggtc aattgcgtat catgttccca atggttgcgc
    15181 tcttgaaaga attccgtgca gcgaaagcag tctttgatga agaaaaagca aaccttcttg
    15241 ctgaaggtgt tgcagttgcg gataacatcc aagttggtat catgatcgag attcctgcag
    15301 cggctatgct tgcagaccaa tttgctaaag aagttgactt cttctcaatt ggtacaaacg
    15361 acttgatcca atatacaatg gcagcagacc gtatgaacga acaagtttca tacctttacc
    15421 aaccatacaa cccatcaatc ctacgcttga ttaacaatgt gatcaaagca gctcacgctg
    15481 aaggtaaatg ggctggtatg tgtggtgaga tggctggtga ccaacaagct gttccacttc
    15541 ttgtcggaat gggcttggat gagttctcta tgtcagcaac atctgtactt cgtacacgca
    15601 gcttgatgaa gaaactcgac acagctaaga tggaagagta cgcaaaccgt gcccttacag
    15661 aatgctcaac aatggaagaa gttcttgaac ttcaaaaaga atacgttaat tttgattaat
    15721 cgaaaagtcc ctgcaactca gttacaggga ctttttttga tattttaaaa agaattttca
    15781 agaaaatctt tcttataaaa agtccaacct tgaaaaagta gtggtcagaa caaaaaatac
    15841 ttaaatggtt cataaaattc ttgacaagtt ggagatttag gagtaaacta ttaaccagtt
    15901 aagtaataga gaggagtttc tgcaatttag aaatgaattg caactagaaa tatcaaatag
    15961 aaagagagtt tcgatgaaaa ttaataagaa ataccttgtt ggttctgcgg cagctttgat
    16021 tttaagtgtt tgttcttacg agttgggact gtatcaagct agaacggtta aggaaaataa
    16081 tcgtgtttcc tatatagatg gaaaacaagc gacgcaaaaa acggagaatt tgactcctga
    16141 tgaggttagc aagcgtgaag gaatcaatgc tgagcaaatc gtcatcaaga taacagacca
    16201 aggctatgtc acttcacatg gcgaccacta tcattattac aatggtaagg ttccttatga
    16261 tgcgattttc agtgaagaat tactcatgaa agatccaaac tataagctaa aagatgagga
    16321 tattgttaat gaggtcaagg gtggatatgt tatcaaggta gatggaaaat actatgttta
    16381 ccttaaggat gctgcccacg cggataacgt ccgtacaaaa gaggaaatca atcgacaaaa
    16441 acaagaacat agtcaacatc gtgaaggtgg gactccaaga aacgatggtg ctgttgcctt
    16501 ggcacgttcg caaggacgct atactacaga tgatggttat atctttaatg cttctgatat
    16561 catagaggat actggtgatg cttatatcgt tcctcatgga gatcattacc attacattcc
    16621 taagaatgag ttatcagcta gcgagttggc tgctgcagaa gccttcctat ctggtcgggg
    16681 aaatctgtca aattcaagaa cctatcgccg acaaaatagc gataacactt caagaacaaa
    16741 ctgggtacct tctgtaagca atccaggaac tacaaatact aacacaagca acaacagcaa
    16801 cactaacagt caagcaagtc aaagtaatga cattgatagt ctcttgaaac agctctacaa
    16861 actgcctttg agtcaacgac atgtagaatc tgatggcctt gttttcgacc cagcgcaaat
    16921 cacaagtcga acagctagag gtgttgcagt gccacacgga gatcattacc acttcatccc
    16981 ttactctcaa atgtctgaat tggaagaacg aatcgctcgt attattcccc ttcgttatcg
    17041 ttcaaaccat tgggtaccag attcaaggcc agaacaacca agtccacaac cgactccgga
    17101 acctagtcca ggcccgcaac ctgcaccaaa tcttaaaata gactcaaatt cttctttggt
    17161 tagtcagctg gtacgaaaag ttggggaagg atatgtattc gaagaaaagg gcatctctcg
    17221 ttatgtcttt gcgaaagatt taccatctga aactgttaaa aatcttgaaa gtaagttatc
    17281 aaaacaagag agtgtttcac acactttaac tgctaaaaaa gaaaatgttg ctcctcgtga
    17341 ccaagaattt tatgataaag catataatct gttaactgag gctcataaag ccttgtttga
    17401 aaataagggt cgtaattctg atttccaagc cttagacaaa ttattagaac gcttgaatga
    17461 tgaatcgact aataaagaaa aattggtaga tgatttattg gcattcctag caccaattac
    17521 ccatccagag cgacttggca agccaaattc tcaaattgag tatactgaag acgaagttcg
    17581 tattgctcaa ttagctgata agtatacaac gtcagatggt tacatttttg atgaacatga
    17641 tataatcagt gatgaaggag atgcatatgt aacgcctcat atgggccata gtcactggat
    17701 tggaaaagat agcctttctg ataaggaaaa agttgcagct caagcctata ctaaagaaaa
    17761 aggtatccta cctccatctc cagacgcaga tgttaaagca aatccaactg gagatagtgc
    17821 agcagctatt tacaatcgtg tgaaagggga aaaacgaatt ccactcgttc gacttccata
    17881 tatggttgag catacagttg aggttaaaaa cggtaatttg attattcctc ataaggatca
    17941 ttaccataat attaaatttg cttggtttga tgatcacaca tacaaagctc caaatggcta
    18001 taccttggaa gatttgtttg cgacgattaa gtactacgta gaacaccctg acgaacgtcc
    18061 acattctaat gatggatggg gcaatgccag tgagcatgtg ttaggcaaga aagaccacag
    18121 tgaagatcca aataagaact tcaaagcgga tgaagagcca gtagaggaaa cacctgctga
    18181 gccagaagtc cctcaagtag agactgaaaa agtagaagcc caactcaaag aagcagaagt
    18241 tttgcttgcg aaagtaacgg attctagtct gaaagccaat gcaacagaaa ctctagctgg
    18301 tttacgaaat aatttgactc ttcaaattat ggataacaat agtatcatgg cagaagcaga
    18361 aaaattactt gcgttgttaa aaggaagtaa tccttcatct gtaagtaagg aaaaaataaa
    18421 ctaatgaaaa atgaaagtct cgataaagag gctttcattt ttattatgta tatatgtaaa
    18481 attcttgaca agcaatatta aaaagagtaa actattaact agttaattaa ccggtttgtt
    18541 actttatagt gaatcaaata tacttaagaa aagaggaaag aatgaaaatc aataaaaaat
    18601 atctagctgg gtcagtagct acacttgttt taagtgtctg tgcttatgaa ctaggtttgc
    18661 atcaagctca aactgtaaaa gaaaataatc gtgtttccta tatagatgga aaacaagcga
    18721 cgcaaaaaac ggagaatttg actcctgatg aggttagcaa gcgtgaagga atcaatgccg
    18781 aacaaatcgt catcaagata acagaccaag gctatgtcac ttcacatggg gaccactatc
    18841 attattacaa tggtaaggtt ccttatgacg ctatcatcag tgaagaattg ctgatgaagg
    18901 atccaaacta ccagctaaag gacgaggata ttatcagtga aatcaagggt ggttatgtga
    18961 ttaaggtaga tggaaaatac tatgtttacc ttaaggatgc agctcatgcg gataatgtcc
    19021 gtacaaaaga agaaatcaat cggcaaaaac aagaacatag tcagcatcgt gaaggaggga
    19081 cttcaacaaa cgatggtgcg gtagcctttg cacgttcaca aggacgctat actacagatg
    19141 atggttatat ctttaatgct tctgatatca ttgaggacac gggtgatgct tatatcgttc
    19201 ctcacggcga ccattaccat tacattccta agaatgagtt atcagctagc gagttagctg
    19261 ctgcagaagc cttcctatct ggtcggggaa atctgtcaaa ttcaagaacc tatcgccgac
    19321 aaaatagcga taacacttca agaacaaact ggctaccttc tgtaagcaat ccaggaacta
    19381 caaatactaa cacaagcaac aacagcaaca ctaacagtca agcaagtcaa agtaatgaca
    19441 ttgatagtct cttgaaacag ctctacaaac tgcctttgag tcaacgccat gtagaatctg
    19501 atggccttgt tttcgaccca gcgcaaatca caagtcgaac cgccagaggt gtagctgtcc
    19561 ctcatggtaa ccattaccac tttatccctt atgaacaaat gtctgaattg gaaaaacgaa
    19621 ttgctcgtat tattcccctt cgttatcgtt caaaccattg ggtaccagat tcaagaccag
    19681 aacaaccaag tccacaatcg actccggaac ctagtccaag tctgcaacct gcaccaaatc
    19741 ctcaaccagc tccaagcaat ccaattgatg agaaattggt caaagaagct gttcgaaaag
    19801 taggcgatgg ttatgtcttt gaggagaatg gagtttctcg ttatatccca gccaaggatc
    19861 tttcagcaga aacagcagca ggcattgata gcaaactggc caagcaggaa agtttatcgc
    19921 ataagctagg agctaagaaa actgacctcc catctagtga tagagaattt tacaataaga
    19981 cttatgactt actagcaaga attcaccaag atttacttga taataaaggt cgacaagttg
    20041 attttgaggc tttggataac ctgttggaac gactcaagga tgtctcaagt gataaagtca
    20101 agttagtgga tgatattctt gccttcttag ctccgattcg tcatccagaa cgtttagg
//
LOCUS       DAVBBS010000042        20009 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVBBS010000042 DAVBBS010000000
VERSION     DAVBBS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20009
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.042"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(379..591)
                     /locus_tag="VQU32_001447"
     CDS             complement(379..591)
                     /locus_tag="VQU32_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472887.1"
                     /translation="MMVVLISVIVAFLYHKITEYVNNRKIDIVIFLSLLISHFIWNYY
                     FLPIEILIAISGANLLLSFLKKLQIK"
     gene            complement(610..969)
                     /locus_tag="VQU32_001448"
     CDS             complement(610..969)
                     /locus_tag="VQU32_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472888.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WILPILLMAGMGDVIIN"
     gene            complement(947..1285)
                     /locus_tag="VQU32_001449"
     CDS             complement(947..1285)
                     /locus_tag="VQU32_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HET2472889.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKINGTRITK"
     gene            1751..2614
                     /locus_tag="VQU32_001450"
     CDS             1751..2614
                     /locus_tag="VQU32_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2472890.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKK
                     EVEIYYQAKER"
     gene            complement(2666..3238)
                     /locus_tag="VQU32_001451"
     CDS             complement(2666..3238)
                     /locus_tag="VQU32_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET2472891.1"
                     /translation="MKSITKKIKATLAGVAALFAVFSPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYSAE
                     NQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            3480..4151
                     /gene="sdaAB"
                     /locus_tag="VQU32_001452"
     CDS             3480..4151
                     /gene="sdaAB"
                     /locus_tag="VQU32_001452"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HET2472892.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            4160..5032
                     /gene="sdaAA"
                     /locus_tag="VQU32_001453"
     CDS             4160..5032
                     /gene="sdaAA"
                     /locus_tag="VQU32_001453"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HET2472893.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNEKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            5054..5686
                     /locus_tag="VQU32_001454"
     CDS             5054..5686
                     /locus_tag="VQU32_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2472894.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            5712..5810
                     /locus_tag="VQU32_001455"
                     /pseudo
     CDS             5712..5810
                     /locus_tag="VQU32_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(6034..7884)
                     /locus_tag="VQU32_001456"
     CDS             complement(6034..7884)
                     /locus_tag="VQU32_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET2472895.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMVDFWGLSASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVARAVDEAKVPKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(7952..9091)
                     /locus_tag="VQU32_001457"
     CDS             complement(7952..9091)
                     /locus_tag="VQU32_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2472896.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKGKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDAEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGGLILKIDAHSKVTESFVMNNVAVIQQGEFVCGGPRP
                     TIVKGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLSLLTLLTLLKHK
                     NGFLIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKGTVIYLEK"
     gene            9149..10315
                     /locus_tag="VQU32_001458"
     CDS             9149..10315
                     /locus_tag="VQU32_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HET2472897.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTRLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TAMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIDILMTVALFTVDRADKIL"
     gene            10454..10780
                     /locus_tag="VQU32_001459"
     CDS             10454..10780
                     /locus_tag="VQU32_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET2472898.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            10767..11360
                     /locus_tag="VQU32_001460"
     CDS             10767..11360
                     /locus_tag="VQU32_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HET2472899.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEVAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            11353..12264
                     /locus_tag="VQU32_001461"
     CDS             11353..12264
                     /locus_tag="VQU32_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HET2472900.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            12327..13391
                     /locus_tag="VQU32_001462"
     CDS             12327..13391
                     /locus_tag="VQU32_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472901.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIETPEGGEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(13463..14323)
                     /locus_tag="VQU32_001463"
     CDS             complement(13463..14323)
                     /locus_tag="VQU32_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HET2472902.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHIYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTETDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPPSFYHQLDANQIEVQPILD
                     KDFKTLAQEK"
     gene            complement(14445..15431)
                     /locus_tag="VQU32_001464"
     CDS             complement(14445..15431)
                     /locus_tag="VQU32_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HET2472903.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(15703..17178)
                     /locus_tag="VQU32_001465"
     CDS             complement(15703..17178)
                     /locus_tag="VQU32_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HET2472904.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(17282..18205)
                     /locus_tag="VQU32_001466"
     CDS             complement(17282..18205)
                     /locus_tag="VQU32_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2472905.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(18219..19148)
                     /locus_tag="VQU32_001467"
     CDS             complement(18219..19148)
                     /locus_tag="VQU32_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2472906.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(19467..19817)
                     /locus_tag="VQU32_001468"
     CDS             complement(19467..19817)
                     /locus_tag="VQU32_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472907.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETADKDVLENSQEELQKAVLFLADEKGSEHTEAELI
                     DNLKEVIAKLKANA"
BASE COUNT         6065 a         4276 c         3622 g         6046 t
ORIGIN      
        1 gtttcctaac aagtgatcgt tgactttgtt tatagcactt agttacctag ataagatatc
       61 ctttagcttg cttccaaaat ttaagtctgc acctataata tgagttcata ttagtactgc
      121 taaatcctat ctatagtaat gtcaaacacc taaataaagt catctctcca attcattcaa
      181 gaaatgaaga aaggcaaaaa atctttaaaa taagaaaagt gcatattatc aggtgttcaa
      241 aaatcttgat aatatgcgtt atataataga aaaatttact caatttcact ttcaaattga
      301 gttttttccc aacctcgttt ttaatttata tcttcaataa aatctaatga cctagttgat
      361 atgtcatatg gaataatatt atttaatctg taattttttt aaaaaactta ataataaatt
      421 tgctccactg attgctatca atatttcaat tggtaaaaaa tagtaattcc aaataaaatg
      481 agaaatcagc aatgatagaa aaataacaat atcaattttc ctgttgttga catactctgt
      541 aattttatgg tacaggaatg caacaataac tgaaattaaa acaaccatca taatactatt
      601 atcctattct caattgatta tcacgtcgcc catcccagcc atcaataata ttggtaaaat
      661 ccaatcagct ccagcaccaa ttccagcaga tgcaatactt ctaataacag tatttgcaaa
      721 agcatttcca atatgaattc ctaatactcc taaaccttta acaatcccag ctaccagtgc
      781 ggagcgtaat ccataagatg caagaactag ttgaaggcct ccacctcctg ctgctgctaa
      841 tgctgcatat gctgcaccag taatggctcc tgtaattgcc cacctgggta tatatatagc
      901 ccccccatca aggtacatca tctcttcttc atcaatcaca acataattat ttggtaatac
      961 gagttccatt tattttctcc tttatactat gtaataccac aaaatgattt tgacctaaat
     1021 cattcttaac ttgaagaatc gcaggaaatg ttaattttgg agtcaaatct tccagtgcta
     1081 ctcgaacagc ttttactgta aaattcaact tattcagacc tgaaacaata ccactgacag
     1141 tcgttccata catgtctgta ccaataattt cccgaatctt catgattgat agttcctttt
     1201 tgtaagataa aagaactgtt gaaacaactg ccgcagcaca atctgactga tcatgttgca
     1261 tcgtacaagg atattttttt aacatagaaa tactcaattc tttagaatta atctaacttt
     1321 tctttgatgt cacccaaacc tcaatcactg cccagtagtt cttgaatcag tttattttca
     1381 atcgtatcat tctttgttct tgatatcttt ccaagcattg tagtacagca gatttttgtc
     1441 caaggactaa gcctccttta acaactcaag ctcccttaca ttatatcgac cagccccatg
     1501 gaaagattat ggccagacta atcaatgata taatacatac tagaatacaa aatatttttt
     1561 tattcatact ttttctccta tctttatttt ctacctacat catacctctt attaagctca
     1621 ttttaaaatt acgttaactt ttttcacaaa tttatttcga ctttcttttt tactttttac
     1681 attgctatga tacaatattt ttaacaaaat tttattgttt gttaactttt ttcacagaaa
     1741 ggagaaaatc atgcgttatg atttcggaaa ggtctataaa gaaatacgtg agtcaaaggg
     1801 gctgacccaa gaagaggtct gtgggggtgt tctctcaaga accagcttat caaaaatcga
     1861 aagtggcaag acaaccccaa aatatgaaaa tatggaattt cttctccgtc aaatcaatat
     1921 gagttttgaa gagtttgaat atatctgtca actttatcaa ccaagtcaac gcacagagat
     1981 tatgcaaacc tatctcaata tgcgttccat tatcgggacc agcgatcttg tcaatttatt
     2041 tcaaaaatgc caagactacc tcaagaccca ccacgatctc cctatagaag aaatcagaga
     2101 tatgcttgaa gttgttattt atctccgtca acacggggca ggggaactat caaaacacgc
     2161 agaacaggtt gtcaaaaaac tttggaaaaa aattgaaaaa caagatacat ggtatgagag
     2221 tgatctaaaa atcctcaata ccattctttt cagttttccc attgaatacc tccatctcat
     2281 cactggaaaa atattgcaac gcctagaagt ctataaaaac tatcaacatt tatatgactt
     2341 gcgaatgaca attctattaa acctttccac actctatcta tacaatcaag ataaaaacat
     2401 gtgtaagcaa atctgctaca ctttactgga ggacgccaag aataagaaaa gctacgatag
     2461 gctggctatc tgctatgtcc gtattgggat ttgtacagac gattctaaac ttatccaaaa
     2521 agggttctcc cttctggagc taaccgagga aacttctatg ctgtctcatc tcaaaaaaga
     2581 agtagagatc tattaccaag cgaaggaaag ataaaaaagc cgagggattt cctcgactct
     2641 ttacatatta tttgttcgtt agttcttaat accagccatt gttaagccag aagtttttag
     2701 cagcagtcca tgaaccgtaa cgtcctgcaa cgtaggcatc tgctacacgt tcttggtttt
     2761 cagctgagta gtcaccgttc aggtatgaat ctgttaattg gtaacgtccg atataacgtc
     2821 catttgtagc tgtatagcta ccacctgatt ctttttgagc gatccattct ttggcttctg
     2881 cttcagatcc gcttacagtt gaaacaactg tttcacttac tactggagct gaaacagttt
     2941 catcttgagc ggctggtgcc gcataagtag ctggcgctgg tgttgcaaca ggcgctacag
     3001 ggccatcgat aaccaactct tgatcaacat aaatcaaatg aatgttatca atgtggttgt
     3061 tttctgccaa tttttcaact gttgtgttgt gagtttcagc gatttctgaa agtgtatcac
     3121 cttctttaac agtgtaagtt gatgattctt gagcagatac aaatgatgga gaaaatactg
     3181 caaacaaggc agctactcct gcaagagttg ctttaatctt tttagttatt gatttcatat
     3241 tcgaaaattc tccttctttc tatagttcta tcataccctt taaatattac tgtttcttga
     3301 cacttttatg tagaaatatt acaaaattat tttttatttc cctaaataag gtgttttttg
     3361 tcataaaaga tactattttt atgctatttg aggtattaga aaggttttca tccacaatta
     3421 aagccaagcc cctcattctt tgatataata gagataagaa tttttataag gggaaactga
     3481 tgaaatcact tcgttttcaa tctgtctttg atatcatcgg accagttatg attggcccat
     3541 ctagtagcca taccgctggt gctgttcgta ttgggaagat tgtctcttcc atttttgatg
     3601 atactccgac agaagttgaa ttccaacttt ttaactcatt tgccaagacc tatcgtggtc
     3661 acggaacaga cctagccctt gttgcaggta ttttaggaat ggatacagat gatcctgaaa
     3721 ttccaaatag tctggaaatt gcccacaagc gtggtatcaa gattgtctgg accattcaga
     3781 aagacagcaa tgcccctcac cctaacacca ctaaaattac tgttaaaaat gcccacaaga
     3841 ccatcagcgt gactggtatt tctatcggtg gaggaaatat tcaggtaacc gaactcaatg
     3901 gttttgccgt ctctctcaat atgaatacac cgactatcat catcgttcat caagatattc
     3961 caggtatgat tgcccttgtt acagaggcgc tttcccgcta tggtatcaat atcgcccaga
     4021 tgaatgtcac tcgtgaaaaa gctggtgaaa aagccattat gattatcgaa gttgacagtc
     4081 gcaactgtga tgaggctatc gaagaaattc gaaaaatccc tcatctccac aatgtcaatt
     4141 tctttaaata ggaggaagca tgttttattc tatcaaagaa ttggtcgagc aagcagatct
     4201 ggactttcaa ggaaatgtcg cagaactcat gattacaaca gagtttgaat tgaccggtcg
     4261 cgaacgtgaa gaagtcttcc ttctcatgga acgcaatctg gaagttatga aagcctctgt
     4321 ccaacttggc ctcaatgaaa ataaatctcg tagtggcctg acaggtggag atgctgccaa
     4381 attggatcac tacatcaaaa acgaaaaaac tttatcagat tacacgattc tctctgctgc
     4441 ccgaaatgct atcgcagtca atgaacacaa tgctaaaatg ggcttggtct gcgctactcc
     4501 taccgcagga agtgccggtt gtctcccttc cgttctcact gctgctattg aaaaattaga
     4561 cctcagccac gagcaacaat tggatttcct ctttgctgct ggtgcctttg gactagtcat
     4621 cgcaaacaat gcctccatct caggtgctga gggtgggtgt caagctgaag ttggttcagc
     4681 ctctgctatg agtgctgccg ccttgactct ggctgcaggt ggaacacctt atcaggccag
     4741 tcaagctatt gcctttgtca ttaaaaatat gctaggcctc atctgtgacc ctgttgcagg
     4801 tttggtcgaa gttccctgtg tcaaacgtaa tgccatggga gctagctttg ctttcatcgc
     4861 agcagacatg gccttggcag gtatcgaatc taaaatccct gtggatgaag tgatcgatgc
     4921 catgtaccaa gtaggagcaa gcatgccaac tgcctttcgt gaaacagctg aaggtggact
     4981 cgctaccacc cctactggtc gtcgcctcca aaaagagatt ttcggagaat aagcttatca
     5041 aataggagaa accatgacct ctatcacagc gatttttttc gatttggatg gaaccctcgt
     5101 tgatagttct atcgggattc acaatgcctt tacctatacc tttaaagagc tgggggtgcc
     5161 tagccctgat gccaaaacta ttcgtggttt tatgggacca cctctcgaaa gtagttttgc
     5221 gacctgcctg tccaaagacc aaatttctga agccatgcag atatatcgtt cttactataa
     5281 ggcaaaaggc atctatgaag ctcaactctt tcctcagatt atagacttgc ttgaggagtt
     5341 atcgagcagt tatccactct acatcaccac gacaaagaat acatctactg ctcaagacat
     5401 ggccaaaaac ttggaaatcc atcatttctt tgatggtatt tatggttcta gccctgaagc
     5461 accccataag gcagatgtca ttcaccaagc cttacagaca catcaactag caccagaaca
     5521 agccatcatc atcggagata ccaagtttga tatgctggga gctcgagaaa caggtattca
     5581 gaaattggcc gtcacttggg gatttggaga gcaagcagat ctactaaact atcaacctga
     5641 ttatatcgct cacaaaccct tagaagtttt ggcgtatttt caatagcata gactgggcga
     5701 caactctatt tcagaagaaa atgaagtaat actcaatgaa aatcaaagag caaactagga
     5761 aactagctgc gggctgctca aaacactgtt ttaaggttgt agatggaact gacgaagtca
     5821 gctcaaaaca ctgttttgag gttgtggata gaactgacga agtcagctca aaacactgtt
     5881 ttgaggttgt ggatagaact gacgaagtca gtaaccatat acggcaaggc gacgttgacg
     5941 tgatttgaag agattttcga agagtataag tctctacata ataaaacgca tattaccaag
     6001 gttatttaac ttgataatat gcgtttttct tttttattca atgtgggttg tttgattagc
     6061 aaaggcgata atagcttgct tcaactcatc tccactgaga gtgcggaact ctccaatctt
     6121 ttgattgatg gattctaaag gcatgacatt aaccttacca acgaaaatct tatccataac
     6181 ttgattatca acgagttcgg ttgataccaa gagttcttcg tagagttttt cacctgggcg
     6241 gattccaact tcaacgattg gaatttcact ttcagtgtgg ccacttagaa gcaccatctt
     6301 cttggccaag tcataaatct tgactggttt gcccatatca aggataaaga cttccccatc
     6361 tttggcataa gcaccagcat ggataaccag acggctagct tctggaatgg tcataaagta
     6421 acgggtcatg cggaagtctg tcaccgttac aggcccacct tcagcaatct gacgttcaaa
     6481 gactggaatg acactaccac ggctaccaag aacattccca aaacgaactg cacagtaggt
     6541 tgattggcta cgttggttaa agccagtgac aatcaactcc gccacgcgct tggttgctcc
     6601 cataacattt ggtggattga ctgccttatc tgtcgaaatc ataaccatct taggcacctt
     6661 ggcttcatca acggctctag caacattgta agttccacgg atattgtttt tgaaggcttc
     6721 ttttggattg cgctccatca taggaacatg cttgtgggct gccgcatgat aaacaatagc
     6781 aggtttgtac tgctcaaaga cttgcaacaa acgatcatag tcttgaatgt ccgcaatcac
     6841 aggtacataa tcaatccctt ggaacttacg aatcaattca tgataaacaa ggtagattga
     6901 gttttcccca tgaccgagca agacaatgcg ttcaggattg aagcgactaa cttgacgaca
     6961 gatttcagaa ccgattgaac ctccagctcc tgtgactaag atggtcttac ctgtcagttc
     7021 tgcacccaga cgcgattcgt caagacggat ttcctgacga cccaaaaggt ccgtaatatc
     7081 aattttttgg aagccagtac ctgcttggtg aaggccctga acaacagttt caaccttagg
     7141 catcttgtaa catttgacac ccagcttatt acacatctgc aagatacgct catattctga
     7201 cggatccagc gacggaatcg caacgatgac acgctcgatt tgatggcgtt tggctaattc
     7261 aggcagattg tcataagagc ccaaaacagg aataccacca agtttttgac cctttttctt
     7321 agaatcctta tccaaaatac cgaccagttc taattcactg gttggatgtt ggtaactatc
     7381 cataaagaga gccccaccat caccggcacc aatcaagaag gtccgacggt gttctccatc
     7441 accactacct tttttgcgtc tggagtagat taactgccaa gtaatccgtg gcaataaaat
     7501 caagaaggta ctcaacaaga taaagagaat gataaaacgg atggagaaga gtggcaagaa
     7561 ggcataacag atactatatg acaagacact gctagcagtc acaccaaaaa agattttcat
     7621 gaaatccgta atcttgctgt aacgactaat actcgcgctc aagccccaaa aatcaaccat
     7681 caattgatag aacaggaagg ccaaactcgt atagataatg tagtcaacag gtgctggatt
     7741 aatcagccca taaaataaaa tataagatac aatgatggaa accaccatac tgaaaatatc
     7801 aaatattccc cagaaaacct gcttttgttg tttatttaaa atttccacca gatcaatcac
     7861 ataatctgtg agttttttat tcatagaaat ttactcctcc ttaaaatagt tctactatat
     7921 tgttattata gcacatttta atttatttgg cttatttttc caaataaatt actgttccct
     7981 tgtactcctt cttccattta aatcctctaa ttaaacctac aatcgtccca aggccataag
     8041 caaagtgaat ggaaaataaa agaaagggca tcacaattag aaatccattt ttatgtttta
     8101 ataaagtcag caaagtgagt aatgacagta atgataaaag tagaaaatag gcacctaata
     8161 gtaaagttat gaatacgaat gtgatcggta acaatgctag actaaacaca agactcaaaa
     8221 caaataaaca aggaacataa tgaaataatg ataaacactt aaactgaaca tgacttgtca
     8281 agccaatcca caaaccattt gaatactttt gatgcagcat tttcttgaat gttggtcgaa
     8341 tatactgata agatagaata cttgggctat agcggatttt ataaccatgt tctcgaattc
     8401 tataatgaat atcattatct tcagttcggc caagttgctc atttactaaa ccaaccttct
     8461 ggaaaacctc tcgtttatac atcccatgaa aaatagaaga aacatatcta tcctcggaac
     8521 tatttcgata attggcaatg ctactgccaa acatattttc ctcaacaaga tgcaaggtct
     8581 ctgcccattt tccttttcct ttgacaatcg tcggtctagg ccctccacag acaaattcac
     8641 cttgttgaat aacagccaca ttgttcatta caaaactctc agtaactttt gaatgagcat
     8701 caatttttaa aataaggccc cctacagaat gcttaactcc caggttaaaa ccactagctt
     8761 gatttttctt aggattgtta tacaatctaa ttgagttaaa ctctgcatct tcttttataa
     8821 attgctgaat gatagctgtg gtcccatctg tggacatagc atttataaat agaatttcaa
     8881 tattctcttt aggataggtt tgatttttta agtcttcaat tagaccaggc agatattttt
     8941 cttcattgta tgcactgatt acaatggata cgagaagact ttcaacattt tgagtcacta
     9001 ctactctcct gaattttccc ttccaccatt ctatcataat tttcaagact tttccatttt
     9061 tatcttgaaa gccagaaacc ttacttgaca ttatagtgaa aaagccttaa aataagctgt
     9121 tattaaaatc agctagaaga gatattatat gaaaaagcaa tcactctttt ttgttccagg
     9181 tattatcctg attggtgttt ccttgcgaac tccttttact gttttaccca ttattttggg
     9241 aaatatttcg caaggtctgg aggtagaagt tagttcgctt ggtgtcttga ccagcctgcc
     9301 tctccttatg tttaccctct tttcaccatt ttctacccga ctggctcaga aaatcggctt
     9361 ggagcatctc ttcacctaca gcctcttctt cttgaccatc ggctcactta ttcgactaat
     9421 caatctgccc ctgctctatc taggaacctt gatggttggg gcaagtgtcg cagtcatcaa
     9481 tgtcctgctt cctagtctta tccaagccaa tcaaccaaag aaaattggtt ttctgaccac
     9541 cttatatgta acgtctatgg ggattgcaac ggctctggct tcctatctag ctgtgcccat
     9601 tacacaagcc agttcttgga aaggacttat cctcctcctc acgttactct gtctagcaac
     9661 ttttttggtc tggctcccaa atcaccgcta taatcataga ctagctccac aaaccaaaca
     9721 aaaaagtcaa ataaaggtca tgcgtaataa acaggtttgg gcaattatta tcttttcagg
     9781 ttttcaatcc ttgatctttt acaccgccat gacctggtta cctaccatgt ctatccatgc
     9841 aggtctatcc agtcacgaag ctggcttgct gacttctatc ttatctctga ttagcattcc
     9901 tttttcaatg accatcccaa gcctgacaac cagtttatct actcgcaacc gtcagctcat
     9961 gctcactctg gtttcactag ctggtgtggt cggcatttcc atgctctttt tcccaatcaa
    10021 taatttcatt tactggcttg ccatccatct cctcatcgga accgcaacca gcgccctctt
    10081 cccttatctc atggtcaact tttcactcaa gaccagcgcc cctgaaaaga cagcccaatt
    10141 gtccggccta tctcaaacag gaggctatat cctagcagcc tttgggccaa ccctctttgg
    10201 ttacagtttt gacctattcc actcttgggt accagctgta gctgccctct tgctcatcga
    10261 tatcctgatg actgtggccc tctttacagt ggacagagcg gataagatcc tttaaacttc
    10321 tagttttagc tagaagtttt ttatatataa aaattttaca catttctctt gacagggctt
    10381 tcttttagat gtacaatgta tgtgtagaaa aattatatat aaaaatctta cacattagaa
    10441 aaggaggttt cccatgtact ttccaacatc ctctgccttg attgaatttc tcatcttggc
    10501 tgtactggag cagggtgatt cttatggtta tgagattagc caaaccatta agctgatcgc
    10561 taatatcaaa gaatccacac tctatcccat tctcaaaaaa ttggaaggca atagctttct
    10621 gacaacctat tctagagagt tccaaggtcg catgcgcaaa tactactcct tgacaaacgg
    10681 tggtatagag cagctcttga ccctaaaaga tgaatggaca ctctatacag acaccatcaa
    10741 tggcatcata gaagggagta tccgccatga caagaactga atacctgact cagctagaac
    10801 tctatctcaa gaaactacct gaagctgacc gtatcgaagc catggactat ttcagagagc
    10861 tctttgacga tgctggagtc gaaggagaag aagaactcat cgctagtttg ggaactccca
    10921 aagaagtggc ccacgaagtt ctatccaatc ttctcgataa aaaaatcaat gaagcacccg
    10981 ctcaaaaaaa taaccgacaa attttacata tcgccttgtt agccctcctt gcagcaccta
    11041 tcggcattcc tctgggaatc gccatcctcg tgaccctgtt cgcaatcctt gtagccgctt
    11101 tgactgtcat tctggctttc tttgcagttt ccatactggg tatcatcggc ggattcctat
    11161 ttttagttga aagtttcact gtcctcgccc aagccaaatc agcctttatc ttgatttttg
    11221 gttctggttt actggctatc ggtgcttctt cgctagtttt acttggcatt tcctatgtag
    11281 ctcgcttctt cggtctactc attgttcgtc tggtacaatt tgttcttaaa aaaggaaaga
    11341 gaggtaatca gcatgcgtaa atggacaaaa ggatttctca tctttggtgt ggtgactacc
    11401 gttatcggct ttatcctgct ttttgtaggt atccaatctg acgggattaa gagcctactt
    11461 tccatgtcca aagaacctgt ctatgatagc cgtacggaaa agctaacctt tggcaaggaa
    11521 gtcgaaaacc tagaaattac tctccaccaa cacacgctca ccatcacaga ctctttcgat
    11581 gatcaaatcc acatttctta ccatccatct ctttctgctc accatgatct tatcaccaat
    11641 cagaacgata gaactctgag tctcactgat aagaaactgt ctgaaactcc gtttctctct
    11701 tctggaattg gtgggattct tcatatcgca agtagctact ctagtcgttt tgaagaagtt
    11761 attctccgac taccaaaagg gagaactcta aaagggatca acatctcagc caatcgcgga
    11821 caaaccacca tcataaatgc tagccttgaa aatgcgaccc tcaatacaaa cagctatatc
    11881 ctccgaattg aaggaagtcg tatcaaaaac agtaaactca caacgcccaa tatcgttaat
    11941 atctttgata cagttcttac agatagtcag ctagagtcaa cagagaatca cttccacgct
    12001 gaaaatatcc aagtccatgg taaggttgaa ctgactgcca aagattatct cagaatcatc
    12061 ctagaccaga aagaaagcca acgaattaac tgggacatct caagcaacta tggttctatc
    12121 ttccaattca caagagaaaa gcctgaatca agaggtacgg aattaagcaa cccttacaaa
    12181 actgaaaaaa ccgatgtcaa ggatcaactc attgcgagat ctgatgataa tattgatcta
    12241 atatccacac caagcagacg ttgacaaaaa cgcttacatc tgctactatg gaagcatatt
    12301 tatctaacta aaaaggaggt tctaccatga aacaagaatg gtttgaaagt aatgattttg
    12361 taaaaacaac aagcaagaac aagcctgaag agcaagctca agaggttgca gacaaggctg
    12421 aagaaacgat agccgatctc gatacaccaa ttggaaaaaa tactcaagta gaggaggaag
    12481 tctctcaagc tgaagtcgaa ctggaaagcc agcaagaaga gaaaattgaa actcctgaag
    12541 gcggtgaagc gagaacagaa atagaagaaa agaaggcatc taattctact gaagaagagc
    12601 cagacctttc taaagaaaca gaaaaagtca ctatagctga agagagccaa gaagctcttc
    12661 ctcagcaaaa agcaaccacg aaagagccac ttcttatcag taaatcttta gaaagtcctt
    12721 atatccccga ccaagctcca aaatctaggg ataaatggaa agagcaagtg cttgattttt
    12781 ggtcttggct agtggaagcg atcaaatctc ctacaagtaa gttggaaaca agtatcacac
    12841 acagttacac agcctttctc ttgctcattc tgttttctgc atcttccttt ttctttagta
    12901 tctatcacat caaacatgct tactatggac atatagcaag cattaacagt cgcttccctg
    12961 agcagctagc tcctttaact cttttttcta tcatctctat cctagtagcg acaacactct
    13021 tcttcttttc attcctcttg ggtagtttcg ttgtgagacg atttatccac caggaaaagg
    13081 actggacgct agacaaggtt ctccaacaat atagtcaact cttggcaatt ccaatcttcc
    13141 tcactgctat tgctagtttc tttgctttct ttgatagcct acgatttacg gccctcttgt
    13201 gtgtgattag cattggaatc attctgcttg ccagtctcca tatcattaca agaccaagtc
    13261 aagcaagtga aaccgactcc ttctatcaat tattcttgtc tgtccttgtg aacggagtta
    13321 ttatcctcct cttctttgta gctgaagtgg cactgattgg agattatctt cgtatcttgg
    13381 cctttcttta aaattcaatc ctgtcatcct tggcaggatt ttttctatgt caaaaaagca
    13441 agtcgattga cctgcttctg ctttactttt cttgtgctaa tgttttaaaa tctttgtcta
    13501 atatgggttg cacttctatt tgattggcgt ctagttggtg gtaaaaagat gggggtaata
    13561 cctctaagaa ttttgcatag tccaagcgag caatggcttc atagtcttct ggtttagagc
    13621 catctcctgt gatttgaacc aagtcaatct cccactgcac ttccttatcc accaattgct
    13681 caatataggc tgcaggaaca aacggttctc tcggtaaaac tgtcttgact ccttgagcta
    13741 gatggtaaac gccatgcact tttccttctc catcctgata gaaggaaact ctcgcaaagt
    13801 aggcatctgt ctcttgaacc gcacgcacac gcgcttcaaa ctgagccaaa ccatctgcct
    13861 ctaaaaagct tttcaagttc tcacgactaa agaaaatagg attaaaaatt ccttggcaaa
    13921 tcgtaaaacg agctacaggg tctgctagat aggcttgaat acttgcttgg aaataagctt
    13981 caaaatcaaa accatctagc ccttcaatat cagtccctgt ataagcaagc aaagcatcta
    14041 aaaatgactt gataatctgc cagtctgtct ctgttagtag gtcaggaaga tcgacacggt
    14101 aagccttttg accatcagca taactgacct taaaaagaaa ttgtgattgc cccactatcg
    14161 cacactcaat atactcgaga cgattaagag gctgacggag atagactaca tcatagatat
    14221 gtgactccaa accgtctagc aatgccaaga tcgacttggc attcaaaatc tcttgttctc
    14281 ctaaaatgct ctgtttattt ggaataaaaa atgttttcgc cataactgga ctcctttgaa
    14341 atcgcttaca tacagtatac cttatttttc tgaaagatac agaaacaaac tttagatttt
    14401 tgagtacaaa gcatagaaaa acagcccctg aggactgttt gatcttatac agtagctgct
    14461 tgatccaagc tttcaccgat agcggctagg cgctcgatca cttcagcttg tgtcaattca
    14521 ttttttgaaa catagcggtt acgtgggtga acacggcact cgtgtgagca tccacgaagg
    14581 tacttgtctt cattttcttc tgatgtcaag atacgacggt tacagaaggg atttccacag
    14641 ttgacataac gttcacatgg tgttccatca aaccagtctt tccctacgat ggttgggttg
    14701 acatggttga catcaacggc gatacgctcg tcaaatacgt acatttttcc atcccaaagc
    14761 tcaccttgaa cttctgggtc tttaccgtaa gttgcgattc ctccgtgcaa ttggccgaca
    14821 tctttgtagc cttcacggac catccagcct gagaatttct cacagcgaac gccacctgta
    14881 cagtaaacca cgacacgctt gtccatgaat ttttccttgt tatcacggac ccattgtggt
    14941 aactcacgga agttgcgaat atctgggcga atagctccac ggaaatgtcc taggtcgtac
    15001 tcataatcgt tacgtgtgtc aaggacaacg gtatctttat caagaagcgc ttctttgaac
    15061 tcttttggag acaagtaagc acctgttgtt tcaagtgggt tgatgtcatt gtcaaagtcg
    15121 ttgtcttcca aaccaaggtg gacaatttct ttcttgtagc gaacaaacat cttcttgaag
    15181 gcttgttcat tttcttcgtc aatcttgaac cagagttctt ccattcctgg aaggctgtga
    15241 acgtagtcca tgtatttttg agttgtttca tagtcacctg aaactgttcc gttaattccc
    15301 tcgtcagcga ctaggatacg gcctttaaga ccgattgatt tacagaaagc caagtggtct
    15361 gcagcaaatt gctctgcatt ttcaattgga gtataaaggt agtaaagtaa gacacgaata
    15421 tcttttgcca taagatttgt tctcttttct attcttaaat tttctaaatt ttttatttat
    15481 ccatactagt atacctgctt gagaaaacga atgcaattta tttgactgga aattgtagag
    15541 gattctcgaa atttcctgaa tggttaaaat aaggacaaga gaaaacatgg atatctatat
    15601 ccttgtgcca aaaaaaccac tgccctcccc agaccaacct gaggaaagca gtgattctta
    15661 ttttaggagt taggaatgaa tacacgaaat caatttagct gattattttt tgtttttcaa
    15721 gaattcatcg tattgttttt gcatttcgtt caatactttt tcgtaggcac cttcagattt
    15781 caatttttcc atcaattctg gaatcgcttt atctgggtct acagtaccag tgttgatagc
    15841 tgtatcaaat tgttgcattg tgttagcaat agctgagatt tcagatttca cattgtcagt
    15901 attgaagata aatccaagcg ctggagattc tttagcttct gccaattctt tcttagaatt
    15961 ttcgatttgt tggtctgtaa cgttttcgtt gatgtaaagg atccagttgt taccagtgtt
    16021 ccatccaccc atgtgagtgt ttcctttgta gccatcaaga acgcgaacac ggttttcttt
    16081 accttcaatt ttttcccagt tcttgccttc tggaccgtaa acaagaccgt tcaaaagttc
    16141 tgggttcgta ttcaagaggt tcaagatttc cattgatttt tctttgttct tagagttgtt
    16201 tgagatgaca aagttagcaa cttgtgttgt ttggtttttc ttgatgaagt tagtgattgg
    16261 tttgatttgg atatctttgt tggcaacacg tgaaagcaag ctgttaccgt agtcagctgg
    16321 tcctactgtt tcttcacgaa cgaaccaagt atcttgttga aggtcaaatg aagtatcgct
    16381 tgttgcgacg tcttttggaa tgtagccagc ttcatagaat ttgtgaagag tcttcaagtg
    16441 ttctttgaaa cgaggcactt cgtaacggtt tacaacttta gtagtatcgc cttcaaggtc
    16501 aataacgaat ggaagcccgt ttgctactgg gtagtcaaaa ttatcagatg ggatgaaaac
    16561 tttaccaata gcaaatggta ctacgtctgg agctttttct ttgatttgtt tcaagactgg
    16621 ctcaagagtt tcgtaagaag taacacctga aatatcgata ccatatttgg caaggagagt
    16681 tccgttgaag gcaaagtttt gagatgatgc tacgttggct gcaactggta cagcataaat
    16741 cttaccattt acagtattac ccttgatata agctggatca agtgccttgt aaaggtcttt
    16801 accttctttt ttgtacaatt ctgtcaagtc agcataagca cctttttgag catttacaat
    16861 atagttatct gcaaaggcga tatcatagtt ttcaccagat gatgtgataa ctgacatttt
    16921 cttaccatag tcaccccagc caaggtattg gatatccaat ttggcaccaa ctttttcttc
    16981 gatgattttg ttggcatttg ctaacaattc atccaagtta tctggtttgt caccgatttg
    17041 gtacattttg ataacaggtt tgtcacctga atcagcagct tttttgctgt tacctgtcaa
    17101 atttccacaa gcagcaagac ctgcagccag agcgactaca ctagcagatg caaaagcata
    17161 ttttttccag tttttcatga taaaaactcc tttttttatt tttaaactta taaacaatgt
    17221 aatgatctta acttcacaca agggaagttg ggaactgaga aatgttcttt ttcaataagc
    17281 actattcttt cacaccaccg atagtcaaac cttttacaaa gtagcgttgg aagaatggat
    17341 acaaaatcgc gattggaagg gttgctacca caaccatggc catacgacct gtttctttcg
    17401 gtagagcaac tcccagttga ccagtcaagc cgaccgcttt ggcaatgtag tccatatttt
    17461 gttggatttg catgagcaaa tattgcaatg gatacaagtt atcactcttg atgtaaagaa
    17521 gggcgttgaa ccagtcattc cagaaaccaa gagctgtcaa aagcgtgatg gttgcgatac
    17581 ctggtagtga caatggcaaa cagatttgga agaaaatccg agcctcactg gcaccatcga
    17641 tacgagccga ttcgagaatg gcttctggaa tggtcttctt gaaaaaagaa cgcatcaaga
    17701 tgatattgaa tggtgagaga agcattggaa caatcaaggc ccaaacagtg tcaccaagct
    17761 gaagtacacg ggtcaccatg atataacctg gtaccaaacc agcgttgaac aacatactga
    17821 gaaggacgaa gatggtaaaa aatctgcgat acttaaaggt tgtccgtgaa atagcgtagg
    17881 cataggttgt tgtgataaag acatttgtca atgtcccaac tacggttaca aagacagaga
    17941 tgaagagggc ttgtaggatt ttatccttaa actgtgccaa aaactcaaaa ccgtctaagc
    18001 caaattggga tgggaagaag ctatagccgt attggaggag gcttttctcg tctgtcactg
    18061 aaataatgat aacgaataca aaaggtagga tacaagagag ggcaatcaaa cccgaaatga
    18121 tactgaagaa gatatctgct ttcttactga aggagtgaat gccgacatta tcaatttttt
    18181 cttttttaat tttctttttt gccatattct cctcctttct agaacaaagc tgagtttgga
    18241 tcgactcgtc ttgcaagcaa gtttgatagg ataaccagaa tcaaaccaac aacggattgg
    18301 taaagaccgg ctgctgcagc cataccgata tctgctgtct gagtcaaacc attaaagaca
    18361 tatacgtcca aaacgttggt tacattgtaa agctgaccag cattgtgtgg gatttgatag
    18421 aagagaccga agtctgcgcg gaagatattt ccgactgcaa ggatggtcaa tacagttaca
    18481 agcggagtca actgaggaat ggttacgttg cgaatacgtt gccacttgct agctccgtcc
    18541 actgtcgctg cttcgtagta ggttggatca attcccatga tcgtcgcata gtacatgaca
    18601 ctgctatatc caaagccttt ccaaatacct aggaaaagta ggagataggg ccagatgccc
    18661 aggtcagcgt agaaattgac ttctttgaga ccaagacttt ccaatagatg attgaacacc
    18721 cctttatcaa tatttaggaa ggcatctgta aagaaactga tgataaccca agacaagaag
    18781 taagggaaca acatagaagt ttgaaaaatc ttcaccattc tcttagaacg gagctcgctg
    18841 aggataatgg caatccctac agatacaact aaacctagaa agataaagcc aagattgtag
    18901 aggacagtat ttcgtgtgat aataaaggcg tctcttgaac taaataagaa tctgaaatta
    18961 tcgagtccga cccatttact atttatgata ctatctatga aaccattact ggtcatgtgg
    19021 tagtctttga aggcaaccac gttcccaaat actggaatgt aaaagaatag aatcaaccag
    19081 agtgcccctg gcaaaaccat caagagaaag atccagttgt ctctcaatgt ttttgaaaac
    19141 tttttcataa tttcctccct ttttattttg atatccatct aaaaattctt ttttagactt
    19201 ttgataacga ttacattatt agtatactcc tatttgcagg ttaggttaaa ctcctaatta
    19261 tagaaaaaac tccacaaatt atgtagcaga tttaaaactt tatcaccact atcaaacaaa
    19321 tgtcctaaat caattgttta ttttatctct attagcccag tgatggcgtc actctgttat
    19381 aagcatccaa caacggggta tactgaaaaa tctccagact agggaactca gcgatagttc
    19441 ctaatctgga gatttttaat atgttattag gcgtttgctt tcaacttagc aataacctct
    19501 ttaagattat caatcaactc tgcttcagta tgctcagagc ctttttcatc tgccaagaac
    19561 aaaactgctt tttgaagttc ttcttgagag ttttcaagga catccttatc tgctgtttct
    19621 ttgcccgctg tttgtcctcc atccttagtt gctgactggc tttccttaat ggactctagg
    19681 gaagcaatgg catctttgac tgtttgcaag atatcacgta aaccttgctc tgtcaaacta
    19741 tcatctgcaa aagctttctt agcctctgcc aaaaccagac gtgctgaatc tgtggtagga
    19801 ttcgatacac ctgtcaatga tctcaaaaga ttttctaaag tttgagtctg cttactaata
    19861 ctagactaaa atcaaaaagc attatataac agtgatatga aatcaactaa agaagaaatc
    19921 caaactatca aaacactttt aaaagactct cgtacagcta aatatcataa acgccttcaa
    19981 atcgttctat agtaaaatga aacaagaac
//
LOCUS       DAVBBS010000043        19766 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVBBS010000043 DAVBBS010000000
VERSION     DAVBBS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19766
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.043"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(481..1206)
                     /locus_tag="VQU32_001469"
     CDS             complement(481..1206)
                     /locus_tag="VQU32_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472908.1"
                     /translation="MKLLKNELIKSKIFLFIIVDICIQILVILAIKTYILDISAPYSE
                     LDYNKYWYVLHALIYMPMIFPIQILYQNLREALIEDNNNGWNIMVINTNNLVKIIYIK
                     VTINIVRCFICYFVYTIFSLIQLGGMGTDMLLTNIIFPNIIFPNIMSFLLFLPIAIFM
                     QICCIRFDSILAKALPNILLILIVLITFQSDWNIFIPATYYYTEIQSTTNLGIKLLVC
                     IWIMGFEFSLLPKLIKLKEQNLV"
     gene            complement(1219..1956)
                     /locus_tag="VQU32_001470"
     CDS             complement(1219..1956)
                     /locus_tag="VQU32_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2472909.1"
                     /translation="MIKLEFLKQKKSILWFVLIFPIILNALLYIDLTFRYRGYLLVHQ
                     NELALSNWQLIFKEQTIFYFSELFYLVLSLIIYEVFAVEFKNDAWLTVISLPFRNKYT
                     INSKLLITVVYTFTFWLSDYISLYVIGKAIDNSLEIGLIFFLKTFTIQLISSLMIMLL
                     YFLTLVLIRKISGIIPIGIIMMILTISIYYNDYNFKIYLPFTYLSHAFRVTESQFYMI
                     LLSNIIIIVLFYILIRKLNERSFEMKL"
     gene            complement(1949..2677)
                     /locus_tag="VQU32_001471"
     CDS             complement(1949..2677)
                     /locus_tag="VQU32_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2472910.1"
                     /translation="MAIVEIINLTKSFKDIEVIHNTSFYLNKGKVYGFVGPNGAGKTT
                     IIKMILGILKPDSGKITIFNQTVEQNSKNILSRIGLVLGPSFYGHLDAYKNLKLIANM
                     KGLSLDTERLNEYLSMVGLKDVKKKKVKNFSMGMKQRLSIAASLLGSPEILIWDEPIN
                     GLDPQGVIEIRSLIRFLQEKKGITFLISSHILSELDKVISDIIIINYGKVEFFGSCHY
                     LLQKYNCRNLEEAYLACLAGGEYD"
     gene            complement(2711..2881)
                     /locus_tag="VQU32_001472"
     CDS             complement(2711..2881)
                     /locus_tag="VQU32_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472911.1"
                     /translation="MKKVNEYVISTAASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(2924..5017)
                     /locus_tag="VQU32_001473"
                     /pseudo
     CDS             complement(2924..5017)
                     /locus_tag="VQU32_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397758.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
     gene            complement(5010..5558)
                     /locus_tag="VQU32_001474"
                     /pseudo
     CDS             complement(5010..5558)
                     /locus_tag="VQU32_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059560.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5639..5701)
                     /locus_tag="VQU32_001475"
     CDS             complement(5639..5701)
                     /locus_tag="VQU32_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHP2/SHP3 family peptide pheromone"
                     /protein_id="HET2472912.1"
                     /translation="MKKISKFLPILVLVMDIIII"
     gene            complement(5870..6858)
                     /locus_tag="VQU32_001476"
                     /pseudo
     CDS             complement(5870..6858)
                     /locus_tag="VQU32_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050775.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6807..7934)
                     /locus_tag="VQU32_001477"
                     /pseudo
     CDS             complement(6807..7934)
                     /locus_tag="VQU32_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423473.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
     gene            complement(8097..8981)
                     /locus_tag="VQU32_001478"
     CDS             complement(8097..8981)
                     /locus_tag="VQU32_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HET2472913.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            complement(9093..9947)
                     /locus_tag="VQU32_001479"
     CDS             complement(9093..9947)
                     /locus_tag="VQU32_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET2472914.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNLTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCNIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            complement(10005..10763)
                     /locus_tag="VQU32_001480"
     CDS             complement(10005..10763)
                     /locus_tag="VQU32_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET2472915.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYALS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVVF"
     gene            complement(10825..11013)
                     /locus_tag="VQU32_001481"
     CDS             complement(10825..11013)
                     /locus_tag="VQU32_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HET2472916.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            complement(11335..11901)
                     /locus_tag="VQU32_001482"
     CDS             complement(11335..11901)
                     /locus_tag="VQU32_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472917.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKTYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            complement(12169..13848)
                     /locus_tag="VQU32_001483"
     CDS             complement(12169..13848)
                     /locus_tag="VQU32_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET2472918.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCNHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKVDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            complement(13940..14101)
                     /locus_tag="VQU32_001484"
     CDS             complement(13940..14101)
                     /locus_tag="VQU32_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472919.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            complement(14785..15099)
                     /locus_tag="VQU32_001485"
     CDS             complement(14785..15099)
                     /locus_tag="VQU32_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472920.1"
                     /translation="MGITWKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYKNLK
                     EKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYDVGE
                     SL"
     gene            complement(15065..15454)
                     /locus_tag="VQU32_001486"
     CDS             complement(15065..15454)
                     /locus_tag="VQU32_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2472921.1"
                     /translation="MPKRYLLDGKTPRISATNIDNGILGYYEDIDDKNYRVFENFISV
                     SFLGAVFYHKYKASLDMKIHCLKLKNKELNKEVAFYLTSIIRQALKNTEYKDQISSTV
                     LPDIKIKLPIDSRGTPDWNYMERYRDR"
     gene            complement(15842..16636)
                     /locus_tag="VQU32_001487"
     CDS             complement(15842..16636)
                     /locus_tag="VQU32_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2472922.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
     gene            complement(16636..17643)
                     /locus_tag="VQU32_001488"
     CDS             complement(16636..17643)
                     /locus_tag="VQU32_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2472923.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            complement(17643..18650)
                     /locus_tag="VQU32_001489"
     CDS             complement(17643..18650)
                     /locus_tag="VQU32_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2472924.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLATFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            complement(18640..19665)
                     /locus_tag="VQU32_001490"
     CDS             complement(18640..19665)
                     /locus_tag="VQU32_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HET2472925.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVISDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
BASE COUNT         6377 a         3631 c         2618 g         7140 t
ORIGIN      
        1 tggacatttt gaactaaaat taatttatta aacatatctc ataagcatat ctattatagg
       61 tgagacaata ttaagaaaga cggttaaaaa acaacctgac tccactggaa ttcaggaatc
      121 aggtcgctta agattttata ttattttact gtctacttga cagggagctg ttcaccaatg
      181 ggaatctata gctttttttg tgactgagaa ctattttatc tcagacttct tcaacacacc
      241 ttattattgg gcacttacgg aaggttaatg aattatttaa aataaaagta aagagaagca
      301 aacagacact ctatagctgt tagacgcttt ttagagtaat aacaatttga taaagtgaat
      361 acaacttgct taattaagcg taagaaacca gaatcataaa atcaattact ttgaagtcat
      421 gactcacgtt gtattgtttc attgttaatc gtattcaatt atttaatttt cgagcaacat
      481 ttaaactaga ttttgttctt tcaattttat tagttttgga agaagggaaa attcaaatcc
      541 cataatccag atacaaacta ataatttaat acctaagttt gtagttgact gaatttctgt
      601 ataataataa gttgcaggta taaatatatt ccaatcactc tgaaacgtga ttaaaacaat
      661 aagtatcagc aaaatattag gtaaggcctt tgctaaaatg gaatcaaatc ttatacagca
      721 aatttgcata aagatagcta taggtaaaaa taacagaaat gacattatat tgggaaatat
      781 tatattggga aatattatat tggttaataa catatcagtt cccataccac ctaattgaat
      841 taaagaaaaa attgtataaa caaaataaca aataaaacat ctcacgatat ttatcgttac
      901 ttttatatag atgattttca cgagattatt agtgttaatc accatgatat tccaaccatt
      961 attattatct tcaatcaatg cctctcttag attttgatac aaaatttgta taggaaaaat
     1021 cattggcata tatattaaag cgtgtaaaac ataccaatac ttattataat caagctctga
     1081 gtatggtgcc gaaatatcta aaatatatgt ttttatagct agaatgacta gaatctgaat
     1141 acagatgtca acaataataa ataaaaatat tttactctta atcaattcat ttttaagtaa
     1201 cttcattttt attacctatt aaagtttcat ctcaaaactt ctttcgttca atttacgaat
     1261 taatatataa aaaagtacga tgattattat attagatagt aaaatcatat aaaattgact
     1321 ttcagtcact ctaaaagcat gagaaagata agtaaatggt aagtaaatct taaaattata
     1381 atcgttgtag tatatgctaa ttgttaaaat catcattata ataccaattg gtattattcc
     1441 agaaattttc cttataagta ctaaagttag aaaatataat aacattatca tcaacgagga
     1501 aattaattga atggtaaatg tttttaaaaa gaaaatcaaa ccaatctcta aactgttatc
     1561 aatagccttc ccaataacgt ataagctaat ataatcagac aaccaaaaag taaatgtgta
     1621 aaccactgta atgagtaatt tagaatttat tgtgtacttg tttctaaatg gcaatgaaat
     1681 tacggttaac catgcatcat ttttaaattc tactgcaaac acttcgtaaa ttattaaaga
     1741 tagaactaaa taaaatagtt cagaaaaata aaatattgtt tgttctttaa aaattaactg
     1801 ccaattagat aaagcaagtt cattttgatg caccaataaa taacctcgat acctaaaagt
     1861 taagtctata tataacaaag cattcagaat tattggaaaa ataagaacaa accataagat
     1921 gctttttttc tgctttagaa actctaattt aatcatattc acctccagct aaacaagcta
     1981 agtaggcttc ttccagattt cgacaattat acttttgtaa aaggtagtgg caagaaccaa
     2041 aaaattcaac tttcccataa tttataatta taatatcaga tatcacttta tccagttcac
     2101 taagaatatg acttgatatt aaaaatgtaa tacctttttt ctcctgtaga aaacggatta
     2161 aacttctaat ttcaattaca ccctgtggat caagtccatt gattggttca tcccaaatta
     2221 atatttctgg actacctaac aatgatgcag ctatacttag acgttgtttc atgcccattg
     2281 aaaaattttt cacttttttc tttttaacat cttttaatcc taccattgat agatattcat
     2341 taagtctttc agtatctaaa ctaagccctt tcatatttgc tattaacttc aaatttttat
     2401 aagcatcaag atgaccataa aatgaaggtc ccaaaactaa tcctatccta gataatatat
     2461 ttttggaatt ctgttctacg gtttgattaa aaatagttat tttcccagaa tctggtttta
     2521 aaatccctag aatcattttg ataattgtag tttttccagc cccatttggt ccaacaaaac
     2581 catatacttt tcccttattt aaataaaatg aagtgttatg aataacttca atatctttaa
     2641 agctttttgt tagatttata atttcaacta tagccatctc tactccttag tttacagcaa
     2701 aaaattatgc ttaattctta ttgtttttgt aaacaatcag cgaacccaat actattccat
     2761 tcaacaaacc taaagaaata ccatattcaa ctggaaaatc taaaaaaatt gcaaatacta
     2821 ttccaatcat cacccctagt gaggctgctg ttgaaattac atattcattt actttcttca
     2881 ttttctttac acctcatttc ctttttgtat ataagctgta gtactatcca ccaagatcca
     2941 ttaagtactt atgatttcct gactcaacaa tcttaccacc tttcattaca aaaataatat
     3001 ccgcatcctt aatcgttgac aatctatgag ctataattat ttgagtacaa tcctgacttt
     3061 gtatatactt tgttattctt tcctcattaa tagtgtctaa tgcactagtt gcttcatcta
     3121 aaattacaat actaggatta tttattaatg cacgtgccag tgctatccgt tgcctttgcc
     3181 cacctgaaat atttgacccc atctctgaga tgatagtatt aaatttcatc ggcatagcca
     3241 tgatttcatc atagatttga actgctttac aaacttcctc tatcttttgt gaagtaactt
     3301 cgttctttaa agttatatta tcaagaatac ttcggttcaa taaaaatgaa tcctgtggaa
     3361 ctactcctaa attttgactt aatatttttt tatctatttg attaatattt acaccatcaa
     3421 ataaaatttt tcctgtatca atcttatata atcctgatag tatttttgat aacgtacttt
     3481 ttcctgaccc cgattgaccg actagtgcaa ttctctgtcc tggctcaata gtcaaattta
     3541 aattttctat tacaggagct gagtctttgg aataagagaa agataggtcc ttaatatcta
     3601 ttctaccttc taaagaacac tttttaagac cattttcaac attctcttca ttttcaagcc
     3661 agatatcatt tactctattt aagtatcctg ctgccaaaat atactacgta taagcattaa
     3721 atattgagat ttctgaacta aataaaattg atgccatcgt ttgaaatgcg acaacctcac
     3781 ccaataaaat ttttccattt aaaacattat caagtccaaa aatcaatata attataggag
     3841 cgaacaattg aaatacatta gtagctgaat tgtataaaat atgcacaata cttcgttttt
     3901 tatactttgt caatacgttt tttaaatact tagaccagtt ttcatatatt tcatcttcta
     3961 atcccgatat ttttattgaa gaaatagtat ttaatgcttc tgtttctact gagtaaatca
     4021 atgattgttc ttgtaattct ctattaaccg tatcatacat tatttttcta gtactaaaca
     4081 gaaacaaaaa attgattaaa ctcaatataa gagctataat tgataatgtt tttgaactaa
     4141 gaaacatata tattacaaca cttagaatag tcccgatatc tactactccc ccaacaactt
     4201 ggttagtaaa tagctcccta aaaccactaa gactagaaat tctgtataaa atatctcctt
     4261 ttgccctgag ttcaaaaaaa ctatatggta gtttaagcaa atgcttaaat gtaaaacttt
     4321 caatatctct actaaaaaat atattagaaa atagaattcc catactccta gccaagatag
     4381 aaataaggta caaacaaagt agagaaaata aaattaaaaa cgaggtttga aaagaaaatg
     4441 tattccctaa tgcagaatta ataaactttg aagtcattac tggtacggaa agtattatta
     4501 agtaacttat tattgaaaat agtaacgtca aaatcaatcg accttttacc tttgaaaaac
     4561 tagagaatac tcttatccat ggagaaggaa actgcgactt caaagattga taattttcgt
     4621 taggaaatga tagcagtact atattactaa aatgttttga aaactcctta aactcataat
     4681 ttatatatcc tttagaagga tccattatat agaccttatt cttttttact ttgtaaataa
     4741 ctacataatg ttgattatcc cagaatgcta ttaatggtaa cggaagttca ctaattttat
     4801 ttacatcttt aacttgatat gagccagtat caaaatttcg tttattcaat aactgtttca
     4861 agtcacctat actataacca tctctccctt tttctatatc acgtcttaaa tctagtaatg
     4921 tctctttgct tttatagaaa tgtagtatgg acaagcagca acataaacca cattctattt
     4981 gagacatttg ctcaatatta ggtacttttc tagtcatcat gtcctcttaa ctccagtaat
     5041 aaaacattaa gtaatctatc atcttgtttt cgagatattt catagacaac tccagatact
     5101 cctagcataa aagaaaatgt attcatagta ttcaaatccg tacttaattc tggtttgata
     5161 acctgagaaa cgacattatc aaaataaaaa tctatagatt cttttattaa taggttttct
     5221 tcatctttca atattgcatc aagacaactg atagtaccta cagaaccgtg acataaattc
     5281 aattctctta ttttttctac attagttaat acattttcaa ttattgataa atcattattc
     5341 ccaatattag ttttatcgaa aatttctttt ctagctaata aaatgcccgg agctccataa
     5401 caccaaaaat taggaaagga atcattatga tttcttcata tgtctttcca attttttaat
     5461 atattattga aacatttatc ttctttatat agtaattcct ctatacattt taatatatct
     5521 gtactaggat taatttcata ataatatgat aattacattg ctactccaga ataatcgtgt
     5581 gctaaacctg ttactgttat attatctaat tgtgaaatac aataattaat acactttttt
     5641 aaatgatgat aatatccatc accaaaacca aaatcggtaa aaattttgaa attttcttca
     5701 tacttggact tcctttccgt ttttatttat tgtacaataa ataaaaacgg atttaatttt
     5761 tgttgggaaa atgggaaatt atgaaatcaa aacttggcat cacacttaga aaagttcgta
     5821 aaggaaaaca aattagtcta tgctctgtag cagatgaaca tctttcaaaa tcacaaatat
     5881 ctcgttttga acgtggggag tcagagatat cttgtattcg actaatcaat attttagaca
     5941 aattacatat tactttgtag gggagttttt ggctatcgtt gctcaattgt ctgattaagg
     6001 tcctaccctt gatgaaacaa ttattatcca tgttttcttt attatagaca aagtaagaag
     6061 acgtttctcg aatgtagact ttatattttt tatgattttc ttcttccata atatccaatt
     6121 gatagttggg aatgaaaata agaccgctct gtttgacacc gaaagacacc ttgatataga
     6181 cgcccttatc aactagcttc tctatttggt tctctgcaag ttccacttca aattcacgaa
     6241 cggtatctca tttttcctta aatgtcttaa aggcttcctc aatctcttca gtggatactt
     6301 tatccttatc tcgttcttct tggaaagcat ggtactgttc ctgtaaattc tctaatcctt
     6361 ctgaagcaac gacttcctta tttttaaaat aatcttgaaa aaatttgaca tcatataatt
     6421 tcttatcact tattttttga tgacccaaac ttatcttttg attattttct tccaggataa
     6481 aagttacatt ttttgtttta agtcaatggt tagattcaat tcttttgctt ttgttattaa
     6541 atcttctaaa gaattgacac ggtttaacaa aaattctaaa cgactttcaa tctcttgctt
     6601 agcaaaatgc gttctaaaaa attcttcatc atatagatct cgtttgctga gttggcgccc
     6661 tcgaattggt tttatcatcg ttctatctgt catcaaaaaa cggctatgct tttgactaaa
     6721 atcaatctga acatgcaact gctttgcttt ctctaaaaaa tcatcaaacg atttagattg
     6781 ctgaagcaaa aaataaagac gttgtttcaa ttcaaattta tgactagatt ccttatattt
     6841 tttataatct cgaaaagatc aggattttta gctaaaactg ccttacgaaa atccttccag
     6901 aactgatggt caatctcatt agccacatcc aaacgccaag catcgatatt aaactcttca
     6961 atccaataag tcgcaacctt taaaagataa ttcttgacct ctggattggc tgtatttagc
     7021 ttaggcatat agtcctcgaa accaaaaaca tgatagggta agtctctctt attaactagc
     7081 ttttcagttg tcaatgggaa ttgttgaata tggaaccaat ccttataagc agactgttca
     7141 ccatttttga cgacattttt tcattgaaga gattgcgaac caatatgatt aaataccgca
     7201 tccagcatga ctttcatgcc acgatgatgc gcttgatcca ccagttcccg aaaggtctcc
     7261 ttgtctccaa aataacggtc aatttcaaag taatctgtcg tattgtactt gtgattactt
     7321 gtagattcaa agatgggaca aagatatagt ccagtaatac ccaagtcttg caagtaattc
     7381 atatgatcaa taatcccctg taaatcacca ccaaagaaat catcgctctt aggtgtgaca
     7441 gatgaatccc agtctaaagt cccttctggg tttaatagag cattgccatt ggcaaatctt
     7501 tcaggaaata tctgatacca taccgtattt gaaacccagt caggaacctt gcaggcatca
     7561 atctcatgaa gataaggcaa cttaaatcca ttcccaattg catgaagatt ttctagagaa
     7621 ttttccacac accctttatc gccatacaaa atattttgac cttctgtatc tctgagctca
     7681 aagagatact ggatacgtgc aaagtcaact gacacttcaa cctgccaatg gtcaaataag
     7741 gtaccagaag ttatcttgac catttctttt gtatcctgat aaaactcttc cataaagata
     7801 aaagggtccc catagtgcaa gttgatgctt tcaatgtccc ctttcttagt tcgaattcga
     7861 atatggagtt tcttatcctt ataaagatag gcatactccg actcaggcct atggtaaata
     7921 gcactcaatt ccatcaattt gtctcctaaa gtattcattc aaattttacg caaacgtttt
     7981 caaactcaat aaatccagta tactattttt gtaaactatt tgcaaggaat gtccgttgtt
     8041 aaaggaacta tcttttaaag tatacaaaaa gccttaaaaa aggctttctc tatatactaa
     8101 tcctctttcg gttttgaaaa tacttctact cgctgagcta ggactttgat ttctacaggt
     8161 aggttatctg atttatctcc gtcaacatct gactccaatt cactatctga agagatttta
     8221 atattacgcg ctttgatata ctcgatatta tcatttgcga caacatcccc ttttaataaa
     8281 tcaggaatga cggataattt ggagaatata gaggcatctt tcagaatcaa aatgttggca
     8341 tagccgtcct tgttttcttc aaagattttc ttatcagcga agtaatttgt caaaaggacc
     8401 aaaacatggc tagcttcacc aacataattt ccattttctg tctcaacctt aatgttaaag
     8461 acctgatctg tcatgacaga cttcatggta tttacagcat aggttaaaat accgaatttt
     8521 gttttgtcct ctatttcaac attgtgaatc gcctcaggca gagaaccgat actaaagata
     8581 taaccaaaat agttgtcatt tgctttaccg atatcaatct tattggttaa atcaaaatcc
     8641 agttcgtcaa ttgcgccatc gatgtcttga ttgatttcca aaagttttgt aatgaggtta
     8701 cccgtaccgc ctgggataat ccctaactta ggaatgtagt ctctctcatc aatacctgaa
     8761 atgacttcat tgacagttcc atctccacca aacacaacca ctgcatcata ctgctcacga
     8821 gaagcttctt cagcaaaatg tgttgcatcc agcgcttttt cggtaatttt ggtttcaaca
     8881 tattcaaagt attcttttgc tttattctcc agcttttctt tgtaatccaa agccttctcg
     8941 ccaccagaag tagggttgat aattaccatt gcttttttca ttgattttat ccttaatttt
     9001 aaacagaaat gtttacattt cgtcgtatgc aagtaaatgt aatcctatta tacaatgaaa
     9061 atacagaaaa gagaaatctg acgtactgga gattaatacg cttttattct attttcccat
     9121 cgcctaacta catcctttaa gggttcatcc aagtaagaat aggccttatc cttgatccaa
     9181 tcaggaatac cgtaagctgc ctctgctatg ttacaagtga ttgctgcgag agtatcactg
     9241 tcgccaccaa gtgagatggc atttcttatc gcatcttcga agtctctact ttcaagaaag
     9301 gcgataatgg cttgagggac agtttcctga catgtttcgt taaaacgata gttaggacgg
     9361 atttcatcta aagtttgaga tagattgtaa tcgtattctt tttctatata atccttaatt
     9421 cgtcttttac actctgtaag attgtcgtta attgattgct cgtactctct acaataacca
     9481 ccaaagtaaa aacgacatag aaagatagca tcagctgtag ccatagcgcc tttgacacct
     9541 tctggatgat tatgagttac ctctgcagaa agactcgtaa gtcctctaga tgatggccat
     9601 ataccagttt tcgcataaaa accacagtcc atgatccaag cacatggaga aatacgcata
     9661 gctgatccat tcccaaagct attataaggc tcacggttat cgctgtttag ccatgcatta
     9721 aaccgagcac cgtaatcagc attcggatac attctgccat atttcttcat cgcgtcaatg
     9781 aaatcgtctt tttgtccacc attcataatt gcttctgcaa cagcacaggt cataaccgtg
     9841 tcatctgtaa aaaagcagtc cttccgaaat aaaggaaagt cctttgtttt gatattgttc
     9901 cattcgtaaa cagaaccgac aatatctcca ataattgctc caagcatcag attcctcctt
     9961 gttcattttg atgcttttta tattggttat ataccatatt tattttagaa aacaacatcc
    10021 tgttggattt taaaaatttc atttttttca aaatagggtt ttaccatttc tttccaccta
    10081 gctctatgaa aattgattga ttttaaagga gatagggcat aatttcccaa tgcataacca
    10141 tcatttactt caacaacaag tgttctgcca tcgcgagtaa caccgatatc tagtccataa
    10201 gctattggcg catctttcca acatgatatc gcttcatcaa ttacacttgc atcaaattgt
    10261 gcatgataat cacctgtata gggtcgaaca tctaatacgc gaccatctaa cacaaaacaa
    10321 cgccattcag ctatgaattc tacaacctca ctaatccata taggatagtc gaaaggtaga
    10381 ccaataccta ttaaatcatg ggttccatta acaactcttc cagtaaagac ttttgaacca
    10441 gctttaggct taataaattt tccccaatta tcaggtatat tcacaatctc tcctaaaata
    10501 ccagcataaa tctttcgacc ataaaactct ttaagctcaa taggatagtc ataaaccgga
    10561 acgtttaagc ccatcatttt tagtaatgct ctagtctcca ttatataatc tacaactata
    10621 tcttcacttg ttaactcttt tatttcagaa aaagattgat ataaaataac ttcttctcct
    10681 tgtaagtagg cacctacttg agcattgtat ttattaattg aaacctcact tggtaattta
    10741 ctttgtctaa tataaacaac catttcatca ctcctatatc actagtgtta cactgatttg
    10801 taaaaaataa tagcaatttt gctcttattt ttttgagtaa atagctccca taatatcatc
    10861 gaaataatca acggtattta ggagtaattc aataacctgg gactttgtta gtcgcattcc
    10921 ccttctatct ctagcatctt ctactaaatt ttcaagtttc tctagatttt tatcatccaa
    10981 gctaatcatt attctatttt tatcggttgc cattttcatc acctcaagtt aattctatca
    11041 caggtgtaac acttgtgtca actggctttt ataatacatt agtttaaaag tggagaggat
    11101 ttttaacaca gtaactttaa atctttggta ttaaaaaatt ttcacaatat ttatagaaat
    11161 aaaatctgtc tcaaatcagt tatcaaatct agtataaatt atgagcggct actctaatac
    11221 tttccctcta aacaagaaaa agacttacac tcaaggattt tcttcccccc cttcgttata
    11281 acgttttgac tcttttacta gcaaaggtat atactcacaa ggaactttgg ttgactattg
    11341 aatctctcca acttcttctt taacatatcc ttctacatct tcaatctcta caaacattgg
    11401 gtctaagtga cacaagaaat gccaaacttc gatccctttt tttctgtaaa gaatcgcttc
    11461 accgtcttca cttccgaaaa agcttctgtc gatttcatat ccgcggcttt ctaagaagtc
    11521 ttttgcttta cgatagttcg tttctcttgt ttcgacatag gttttaactt catggttgtt
    11581 aacgacatat gcatcaattt ttgaatatcc ttcgatcact ctatcatttt tgagggataa
    11641 atttgaaatc tctttccaaa taatgtttac attttcctca ggatcgaaca taaatttaga
    11701 taaaggaaca atatttccgt taaaaataat ttccatataa tccggtatgt ttttaggatt
    11761 aaaatactcc acttcaaaac catcttctgt ttccagagtg tatcccggga tttgagctac
    11821 aaaggctttc ccatcttcta tggaatcaaa tgctactaaa tctttagaat aatcattttg
    11881 gtacaattcc aatataacca tcgataatct ctccattttc attatcaggc taatgtaaat
    11941 aagcacgtca cctgaccaat tcaggctctc tgtatcatct catcatattt cctacttact
    12001 ttacgagtcc tatacccaga acacacctta tcgaccttcg gtctcacctc gttgcattgg
    12061 ctgaacatct acttttactt cgctgatgct tcaactcgta caagcagtga taccgcctca
    12121 gcgtgatgcg tcagtgggac tcaaaaggtt cggggaacct tttgaggatt aactacgttt
    12181 ctctaataaa cttacacatt caacttgttc atcattgtcc aaacctatgt tgagattttc
    12241 ttctataatt ggtagcttaa aagtaatgga ttttagccat tgtccgttag attgtttttc
    12301 ttcataaact tgaatttcag aaatcaaagc tgaaattaac tgcctacgct ctacatcatt
    12361 catgacttta tagagcttat caaaatagat cagaacctta tatatgttat ctcctgtaag
    12421 cttttcagct tcaatagtct gtttctttgc tttcgcatca attagtgatg attctaattc
    12481 atctactttg tcatacatac gatatagtct atcatctaaa tcctgtttcc ttctcttata
    12541 atgcttatct tcaacatcta aattatctat ttcctcaatt agcttaaact ttgtagaatg
    12601 actctttctc aattcctttt ggtaattatc tatttctttt tctatttcag aggtatccac
    12661 cttcatgttg attttttctt gcatcataga agcaaatttc ggattactta ctatcttgac
    12721 aatcacctct gcaacagcat catctaacaa ttcttctcta atttgcttac tgaatgtaca
    12781 cttatgacct cttatcatct gcctatggtt acaaccatag taataaaaat ctttatactt
    12841 tgtgccatct ttctttttct tgatacactt gttcccaaac attcccactc cacatatcgg
    12901 gcattttaca attccagaaa gcaagtgtgt gcgtgtatct tttcctttat tcacatgctc
    12961 atatttcttt gcttgagatt ttagcttaac ctgagcagct tgccaaactt catcggaaac
    13021 tatagcttca tgtatccctt cagatattag atattcatct tgttcaacct gcttatattc
    13081 atttcttgta ccatgaactt tttctaaagt tcttcttcca aatgctattt tcccattata
    13141 tacaggattc tttaatatct ttcttataag acctgcatca aacaaaggat tcttaccatt
    13201 ctgtcttggg atttttctaa ttccatgatt ctctaagtat ttagatatcc cattggctcc
    13261 tatcgtagta tttacatact ggtcgaaaat cgttcttatt gcaactgcct cttcctcatt
    13321 tataaacagc ttgccgtctt caagtttata tccatacgga gcaaagccac cattccattt
    13381 tccttccctt gctttttgaa tgcgaccttc cattgtttga atacggatgt tttctctttc
    13441 tatttcagcc acagctgata aaacagaaat cattagtttc ccagcatctt tagatgaatc
    13501 aatgccatct tcaacgcaga taagattaac tccataatcc tgcattatct gaagtgtaga
    13561 aagaacatca gcggcatttc ttgcaaatct tgataactta aacacaagaa caaaagatac
    13621 tccatctttt ccagatttta tatcttccat cattcgattg aactgtattc taccttcaat
    13681 agacttgcca gacttcccgg catcttcata ctctccaaca atttcataat cgttgtaaat
    13741 agcaaaagct ttcattcgtg atttttgtgc ctctaacgaa tacccctcta tctgtattga
    13801 cgtagatact cgtgtataga ggtatacttt tattttttct tttgacatag tattaatctc
    13861 aatataattt ttctatatca tatataattt ttttaattta agtttggact atcatttcaa
    13921 gtatattata acacttttat tagtccgtct caatttgtgt ttttgccatg tcaaaactat
    13981 ttttcatctc ttgatttttt gctggcgttg gatcgggtag attatctaaa tctaaagcac
    14041 cagcatattt tgcaatcaga tttgctatta aatcagccaa tccattccag tcattgtcca
    14101 atatatacct cctctaaagt tttatatcta ataattattt gtttaattaa gttttttgac
    14161 attgacaagt gctttggatt agcaacatag gaatctcacc tccgcctcta ttccggatga
    14221 gccggcttca accttagaag tatcattatc ctcattttct tcatagcgga tagggtatcc
    14281 ctccctatat tcaaactctt acttatcgct cactttcttt ttacttagca gaactttttt
    14341 tgccgaatta ttcagccgaa agatcttgac ggataggtta ttacgctcca aaaataatta
    14401 acgtcttgtc ttggtctatt caattgttaa ggttcaaaat ttatcgagag ttattaatct
    14461 ttttaaaatt tgaccatcag aaaatattta tcttaatgta acaaaattct ataaattacc
    14521 ctcttatact taacagtgaa aagaagtctt tcttggtaac caattttgaa atagaatttg
    14581 cttatataaa aaggtcaaat tcccactgca taagtagcag tgaaaattag accctcttgg
    14641 taactgtcat ctaaaagtct tctagcattt ctttaatttt ctaatcgcct ttaaagtgtc
    14701 atatttatat ttttttatct ctggcatact ttgatacctt tttagactta aagtctttaa
    14761 tagtgccttt ccacctcttt ttatctataa agattctcct acatcataat tcattttttt
    14821 atttaaacct ttctgtctta gtttgtcttt atcttcttca taccatttta agattgtcac
    14881 atagtggttt tgataggtct taccactgct ttccatgtat ctggatagtt tatttatcat
    14941 tatatctgtg tgtgagttta atttttcttt tagattttta tattcttctt tgcttaacct
    15001 tacatttttg aattctccat aaaaaatggg ggtggacttt ttatctatct ctccctctct
    15061 ctctttatct atctctatat ctttccatgt aattccaatc tggagtacct ctactgtcta
    15121 tcggtaattt aattttgata tctggcaata ctgtgctaga tatttgatct ttatattcag
    15181 tattttttaa agcttgccta ataattgaag ttaaatagaa tgctacttct ttattcaatt
    15241 ctttattttt taattttaaa caatgaattt tcatatctag gcttgcttta tatttatgat
    15301 aaaagactgc tcctaaaaat gaaacagata taaaattttc aaaaactcta taatttttat
    15361 catctatatc ttcgtagtaa cctaagatac cattgtcaat atttgtagca ctaattctag
    15421 gagtttttcc atcgagtaaa tatctttttg gaatagatga gcctgttggt acttgactcg
    15481 atttcccctt tttttcggta ataaatattt ctttttattt tgttgtctga tatttttcct
    15541 acctgtcctt tgtaggatga gtattttcta gattttcttg aataactttt tacttgaagt
    15601 tttagctttt gaactagtcg ttgtactttc tttttgttta ttatcagtcc tgatcttttt
    15661 aatattgctg ttattctcta tatcctattt ttcattcatg atattctttt actaatttta
    15721 tcttaaattc tgtgctgtat ttgccattaa aaaactgacc tcctttagtt agttttttgg
    15781 cctaactttt gagggtcagt tcaaaatttg cgacttttaa atgaattcca atattcaatt
    15841 attaagagct aacatggtgc ttgccaatag gaatcattag aggcgaattg gaaatagggt
    15901 cacgtataat ttttgcttca agattaaaga tatctttaac tagtttatca tttagtatat
    15961 cttcaggctt tccctctgca acaagtttac cttctttaat tgcaaatagg taatcagcgt
    16021 atcttgctgt tagatttata tcgtgcaaaa tcatgcaaat ggttgtctta tatttttggt
    16081 ttagatcagt caagaggtct aatagttcta tttgatatga gatatccaag taagtagttg
    16141 gctcatctaa aagtaggata cttgtatctt gggctagggc tagagctatc catactcttt
    16201 gcctttgacc cccagaaagt tcttcaacta ggttatttgc tagatcttca acattggcct
    16261 taaccattga tctgtttatt atttcaaggt catcttttcc aagactctta aaaggctttc
    16321 tgtaggggaa acgaccacgg cttacaagat cagctactgt tattgattca gggattattg
    16381 gagattgagg taatatagct atgtgttttg ctaaatcttt ttctttataa gaattaattg
    16441 atttattatc aagcaatact tctccctcta atggctttat aagtcgagac aaggttttaa
    16501 tgagtgttga tttcccacaa ccatttgacc caataataac tgatattttt tcttcaggta
    16561 tttttatatt tatattttcc aagattattt tttcatcata accgcaggta agattatttg
    16621 accacagacc tttcattata tattcctcct gttcattttt attagtaagt atattaagta
    16681 tggtgaacct aacaagccag ttacaacacc tactggatat ctagctggta aaatattttg
    16741 agagaatatg tctgataaca aaactagtaa aattccaacc aatccagcta atattgggct
    16801 tcttttcttg ccaatattta aggctatggg accagctaaa aaagatatac aagctattgg
    16861 tcctgtaatt gaagtagaaa aagcagttaa agatacagcg caaaaaatta aaacaagcct
    16921 tgaaagctcg ggatttgctc caagtccgat tgctatttct tcaccaagtt caataatttc
    16981 tagtctttta ttaaaaaata aaactaatat agtagcaata atacttacta ttagaacaag
    17041 aggtatgtca tctaactttg taaaagataa agagccactg agccatctca taacttcttg
    17101 taattcatat cttgctactt tcaacaataa aaatgaggtg cctgctcttg tgacagcttg
    17161 aaaaccaata cctaatatta tcagtcttgc tgctgaaaaa ccatcttttt tagctagtaa
    17221 aaataatatt aaagatgatg ttagtccaca agttattgaa ataattccag tagttaaact
    17281 atttgttttt aataccaata tgcaaaagac cgctgcaata gatgaagaac ttgtgacacc
    17341 gattatatca ggacttgcaa gaggatttct taacatagtt tgaaagataa atcctgccaa
    17401 tccaaaagac cagccagcta taattcctgc taataatttt ggtaatctaa tttccataat
    17461 cgaaaaacta gctccaggaa cagtttcact atttaagact ttaatcaaag ttgaaaaaga
    17521 ataactttca tctccgataa gtaaaatgaa aaatgataga ctgattatta ttaataaaaa
    17581 tagtgaggaa aatagtgtta ttctattttt tcttttttga atacctataa ttaaattttg
    17641 cattagttat taacccctct atttttcata gttacataaa taagtactgg acccccgatt
    17701 attgcagtaa ttatccctac ttcaatttca cctggtttac ctaacatacg gccgattata
    17761 tcacatataa gcaagagctc tgcacctata aaagatgaag aaatggtcat tgtgcgtata
    17821 tctttgctta taaataagcc acaaaagtga ggaactataa gacctacgaa gccaataggt
    17881 ccaccaattg cagtaatact tgaacataaa agcacacttg caattattgc aagtgatctt
    17941 atcctattaa cattaactcc aagaccaaca gccatttcat cacccatagc taaagcgttt
    18001 aaatctgatg aaataaatat agctatcaag tgacctaaaa ttataaaagg tagtagtgta
    18061 gatatagaag ataatgtagc tgctccaagg ctacctattt gccaaaatct aaatttgtct
    18121 aagacgttat tattcggtaa aattaaaaaa cttacaaaac tgcttaaagc catactaaca
    18181 caagttcctg ataaggctag ttttataggg gtaaggcctg cttttccgct tacagcaatc
    18241 gcgtatacaa aaattgcact tactaagcca ccaatgattg caaaacttat atggcttatg
    18301 cttgatgaaa ttcctaaaaa agaaagacca attactacac taagacttgc tcctgtgttt
    18361 ataccgagta tacctggatc agctattggg tttctagtaa ctgattgcat caatacaccg
    18421 cttatggcaa gactagaacc tgctaaaatt gcaaaaatag ttctaggtat tcttttatat
    18481 ataattgatt taataaaatc atcatttgta ttgcccattc caaaagttgc taaaaaatct
    18541 ctgatattaa tttcttttgt tccaagcttt aatgacaaaa atattgtaat aagaagacct
    18601 aggcagatta caaaaattat gcctaggttt atttgttttt tatttcgcat ttttgcatgc
    18661 atttcctaaa agatttaggt attcttcaat agtatagttt attgaaagtg gtgttggagt
    18721 gcatgaggct gctaacggtg tattatctga aattacagca acggcaccat ttttaattgc
    18781 atttatttta cctaaaagag gatctttttg taaagcttca agagttttat catcaccata
    18841 agttatgatt acatcagcat catttatctt atttgcttct tctgcagaaa tttcctttgc
    18901 aaaactatct tcactctcaa attcttttaa tgattcaggg aaaactagac ctaaatctgt
    18961 taaataattt gctcttggat ctttgctagt ataaatccag aattttgatg tatctgcagc
    19021 attaatcata gtaaataata ctttttttcc tttgattttt cctttgattt ctggatgttt
    19081 ttctaattct ttggatatac gagcttcagt atttttgatt aactcatcac cttctttttc
    19141 catacctaag gcttttgaat caattttaat catatctctc cataaagttt gccaaggttt
    19201 agatttgtat gctgctacag gagcaatttt tgatagagtg tcataatctt ctttagttat
    19261 accagaataa cctgctaaga taacatctgg tttagaattt gatattgctt caaagttaag
    19321 tccatccaaa tcgtcaaata ggttagcttt accatttagt tctttgattt tttcttctgt
    19381 ccatggtaaa actcctttat cagcacttac accgtaattt gcttttgaaa atccaacagg
    19441 aactattcct aaagctaatg ctacatcatg atttccccaa gcaatagttg caactctttc
    19501 aggtttttta tctaatatag tttgaccgaa agcatgatct aaaactattt tatcaggcgc
    19561 atgctcttta gaagtatttt cttcattttt aacagaatta gaagaacaag cgctaaaaac
    19621 tatacacata gctaaaatag ctattagaaa aaatttgttt ttcataaaaa ctccttaaac
    19681 atatttcaag tctattgtat cgataataat tatcattgtc aatagataca gttacaaatt
    19741 taataatata attttttcaa aaaagg
//
LOCUS       DAVBBS010000044        19587 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVBBS010000044 DAVBBS010000000
VERSION     DAVBBS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19587
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.044"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(20..667)
                     /locus_tag="VQU32_001491"
     CDS             complement(20..667)
                     /locus_tag="VQU32_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HET2472926.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KQTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLENYIRLPAKLES"
     gene            complement(776..1294)
                     /locus_tag="VQU32_001492"
     CDS             complement(776..1294)
                     /locus_tag="VQU32_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HET2472927.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(1521..2390)
                     /gene="rsmI"
                     /locus_tag="VQU32_001493"
     CDS             complement(1521..2390)
                     /gene="rsmI"
                     /locus_tag="VQU32_001493"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET2472928.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGTSAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQDVEEKDEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(2393..2710)
                     /gene="yabA"
                     /locus_tag="VQU32_001494"
     CDS             complement(2393..2710)
                     /gene="yabA"
                     /locus_tag="VQU32_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HET2472929.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(2750..3640)
                     /locus_tag="VQU32_001495"
     CDS             complement(2750..3640)
                     /locus_tag="VQU32_001495"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET2472930.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(3637..4275)
                     /locus_tag="VQU32_001496"
     CDS             complement(3637..4275)
                     /locus_tag="VQU32_001496"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET2472931.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVKVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            complement(4495..5292)
                     /gene="proC"
                     /locus_tag="VQU32_001497"
     CDS             complement(4495..5292)
                     /gene="proC"
                     /locus_tag="VQU32_001497"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET2472932.1"
                     /translation="MKIGFIGLGNMGASLTKSVLQARPSDEILLANRSQVKVDAFIAN
                     FGGQTSNNEEIFEEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            complement(5296..6558)
                     /locus_tag="VQU32_001498"
     CDS             complement(5296..6558)
                     /locus_tag="VQU32_001498"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET2472933.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAVSFLPRLEQ
                     VLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVTGDGQIRE"
     gene            complement(6568..7698)
                     /locus_tag="VQU32_001499"
     CDS             complement(6568..7698)
                     /locus_tag="VQU32_001499"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET2472934.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMVEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRFQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(7781..9661)
                     /gene="cbpE"
                     /locus_tag="VQU32_001500"
                     /gene_synonym="pce"
     CDS             complement(7781..9661)
                     /gene="cbpE"
                     /locus_tag="VQU32_001500"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HET2472935.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSAYGNWWYQAPDSTGEYAVGWNEIEGEWYYFNQTGILLQNQWKKWNNHWFY
                     LTDSGASAKNWKKIDGIWYYFNKENQMEIGWVQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTGGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SE"
     gene            complement(9664..10551)
                     /locus_tag="VQU32_001501"
     CDS             complement(9664..10551)
                     /locus_tag="VQU32_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2472936.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(10541..11002)
                     /locus_tag="VQU32_001502"
     CDS             complement(10541..11002)
                     /locus_tag="VQU32_001502"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET2472937.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMFLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(10999..11907)
                     /locus_tag="VQU32_001503"
     CDS             complement(10999..11907)
                     /locus_tag="VQU32_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET2472938.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            11900..12097
                     /locus_tag="VQU32_001504"
     CDS             11900..12097
                     /locus_tag="VQU32_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2472939.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            complement(12149..12820)
                     /locus_tag="VQU32_001505"
     CDS             complement(12149..12820)
                     /locus_tag="VQU32_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2472940.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(12874..13095)
                     /locus_tag="VQU32_001506"
     CDS             complement(12874..13095)
                     /locus_tag="VQU32_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HET2472941.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            13367..14176
                     /locus_tag="VQU32_001507"
     CDS             13367..14176
                     /locus_tag="VQU32_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2472942.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(14357..15232)
                     /gene="aguB"
                     /locus_tag="VQU32_001508"
     CDS             complement(14357..15232)
                     /gene="aguB"
                     /locus_tag="VQU32_001508"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HET2472943.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKLLAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYNLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(15242..16327)
                     /gene="aguA"
                     /locus_tag="VQU32_001509"
     CDS             complement(15242..16327)
                     /gene="aguA"
                     /locus_tag="VQU32_001509"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HET2472944.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVV
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(16324..17451)
                     /gene="nspC"
                     /locus_tag="VQU32_001510"
     CDS             complement(16324..17451)
                     /gene="nspC"
                     /locus_tag="VQU32_001510"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HET2472945.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(17451..18710)
                     /locus_tag="VQU32_001511"
     CDS             complement(17451..18710)
                     /locus_tag="VQU32_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HET2472946.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMLD"
     gene            complement(18707..19567)
                     /gene="speE"
                     /locus_tag="VQU32_001512"
     CDS             complement(18707..19567)
                     /gene="speE"
                     /locus_tag="VQU32_001512"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HET2472947.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEYDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
BASE COUNT         5680 a         4507 c         3482 g         5918 t
ORIGIN      
        1 tggtattatc aatcatgaac taggattcta gttttgcagg aagtctaatg taattctcaa
       61 gctgtgagtt cattatgatt tgtaaatccg atatatcttc agcagctaaa aacacatttt
      121 gaaatgttcc aagtccgttt tcaccaaaac tatattctct cttactatac ttactcttat
      181 tattctctat atagttagag tcaaggttct taacctcttg aagttcattt tctttacttc
      241 tgaggttaaa atcttttact tctgaagtta agttttttga cttctgtaag tcattttctt
      301 ttacctgaaa tatactcata aagtctttaa cataaatgat gttcggctta ccaagtccaa
      361 gccttactct ttcgatcagt cctattcctt tttttacatc aagctcatct aatgttttta
      421 tggcagttgg ctttgagata tttcttcttt tcataatttc ttcgacagta aaatagataa
      481 atactctgcc ttccttgtct atccagttat tcttaaatga cattcctgtc tgtttaagaa
      541 gtatggaata aaggataatt gcttctgctg ataagccccg aaattcttct ctatcaacta
      601 atatctccgg tacttttaag aagttaaatc tttccgcttc tctgttataa aaatattcaa
      661 agtccatgca agatcacctc ccttcttaaa aatttgcaaa gaaaaagacg atagtttttc
      721 ttctatcgcc tttggtaacc atttttatga aattttttaa agttaaaaat ataatttata
      781 atactatctt cttccatttt ctgattttag ttctgaaagt tccaaaaggt gcaaccgtat
      841 ttacatgaat aaacttatac acttcccaag tcgctgtttt agtcgcttca tcagcccact
      901 ttctcatatg cggttcaaat aattcttcct cactcaacga atcaatcatt gcagagatag
      961 aattaatatt ttcatttaat tttgctttca actcttgcag agataaatga gcgtaggtat
     1021 ctgtgaacca ctgatataat tcaccaagtt gattccattt aaatttatcc gatggtgttt
     1081 ttacttgaag cccctttctt tcatcttctt cccatttaag aaccaagttg gtccaaccaa
     1141 cctgataagc aaggttttct gctggagttc tgtcaacttc atcagctctc ttatctttta
     1201 aattttctgg aatattatca aattctaaaa tatatttctc aaatgttttc tctatctcag
     1261 cttttagttc ttctttattt tcgtatgttc gcaaaaaatc accttctttc tgatttatca
     1321 atcctttgat ttaaaatgac cttatttttc tatttatacc ttctaagaac gctataactg
     1381 ctgctccaaa catcggtatt ccgtatgggc ttaataaaaa acttaatacc aaagcttcta
     1441 taccaagtga tacatcttta tattatcatc actaattaag ttaaacagaa acagacagaa
     1501 ttattacatc ctgtctccct ttattgtttt tcttcccaat cgtggtaggc agcgtagagt
     1561 tgactcttat tccactggta aatcttagct acttccttaa tagcttgatt tttcttcatg
     1621 ccttgctgga tacgggcttg gatttctaag aacaagtcct cctcgtcttt ttcctccaca
     1681 tcctgactgg caccttcaac aataagaagg cattcgccct tgagtggcgt ttcagcgatg
     1741 ctttccagta attcagaaat tgtacctcgt tggtattctt catagatttt ggtcaattcc
     1801 ctgaccaaaa caaccgagcg gtcaccgtag acttctaaca tattttccaa cgtgtctgct
     1861 acacgatgag gtgattcata aaaaatctgt gtttcaggat aatctttttt agagccaaaa
     1921 aattgcttct gttgacctga ttttctcggt aaaaaaccgt aaaagatatg tggctgtggc
     1981 gctaaaccac tggcaatcaa ggcagaaatt cctgcagagg tacctggaac agtcacaact
     2041 gcaatttctt cctcaatagc tgccttaact aaatcatgac cagggtctga aatgctaggc
     2101 aaaccggcat cagagacctg agcaatactt tgccctgctt tcaagaaacc aatcaaatca
     2161 ggaatttttt ccttggcatt gtgctcatga aaactgatct gcttggtgga aatgtcaaaa
     2221 tgcttgagca aaagccctgt attgcgcgta tcctcagcag caatccagtc cacttctttc
     2281 aaggtctgga tagcacgaaa agtcatatca tctagattgc caatcggcgt tgccactaga
     2341 tacagcttgc catagggaga ctgcccctta aaactttttt gaatttgcat gcttactccc
     2401 tgtataacaa ctcatcacag aacatacatt cttcgtcctg ctctcgacgt tgtccataaa
     2461 aatcattaca tacgtgaaat ccatcacggt aaatacgacg gacactttcg cgaacatgct
     2521 tggccttgac gggagcgtct gcttccacct cacccaagcg ttctcgcaac ttactatttt
     2581 ccaagcgaag agctgtattt tcttctacca agctcttgag atttttcttg atggcttcca
     2641 catcggctaa agttaccaat aattgttggg aaaaatcatc cagcgcgtca aataattctt
     2701 ttttgtccat aaaacagccc tttcctttct ttatcattca tttttgagtt tatatttctt
     2761 tcaagaccag atattccatg gcattttgaa agctgacatt agcttgccac atttttctag
     2821 cttctagtaa atcttgtaga atcactctta ctcttacctg caagaggtcc tgcccacaga
     2881 gaacttcaag aatccgtaaa acctgatcct gtttttcctt atcatctgcc aagttggcta
     2941 atttggcaac ctgtagataa ctttcttttt tcttagctac taaccaagtc agcaggcgtt
     3001 cactttcatc gaccaaggtc caaaaacttg cctgattagc caacttttct gcttcagctc
     3061 gcgattgact aaacttagct aaaagagtcg cttttttctt aacaagtccc atttgttcta
     3121 agagtaagat aagtttttct tcttgctttt taaagtggaa gatctgagtc cgacttcgga
     3181 ttgtcggtaa catcttttcc tcatcgctag tcaagaagaa aatataaact tcactctggg
     3241 gttcttcgat gaccttgagc agagaattgg ctgcgttggg atgcatttta tccgcttgct
     3301 cgatgataaa gacctgttgc tggctttcaa tccctgcttg agaaaactgt cccaccaatt
     3361 cccgaatacg ttctgtcttg atgacctgat tgactggctt aatcaaggtg acatctggaa
     3421 actcttcctg ttcaatcagc ttgcaacttc ggcatttctc acatggtaag acgccaactt
     3481 tatccgtaca aaagaggctc ttagctaaaa attgcgccat ttccaagctt ccaaagaaac
     3541 ctgaaaagag ataggcgtga ttgagctggt cttgttctaa gatacggaca aaacggtcaa
     3601 actgagctgg ttgccaagcc tttagttgat cttgtttcat ttggccaagc ccattccgtc
     3661 aaacaagaca gccttggtag tttccacaac ttgctccaaa gggagactag catcaatctt
     3721 gacaatgcga tttccctctt tatccagaag agaaaggtag ccttgacgaa cttttttatg
     3781 caagtccaac ccttccaaat ccaaacgatt aacctcgcgg tcactattag cagcaatacg
     3841 agccagccct tcttccacct cgatgtcaaa atagagtgtc aaatcgggtt tgaggccatc
     3901 tgtcgcaaac tgattgagcc agtcaatggc ttcaatatct aagccacgac caaatccctg
     3961 ataggcaaca gaactatcga taaaacgatc catgatgacc aacttgccag cttcaagggc
     4021 tggaagaact ttttccacca aatgctgtct gcgactggca atatagagaa gtagctctgt
     4081 tttagcatcc atctgagtat gacttggatc caaaatcact tcccgaatct tctccccaat
     4141 caagactccg ccaggttcac gggtcgtcag cacctttact cctttttcct ctaaaattgg
     4201 tagcagagcc tctaaaacac tggtcttgcc tgctccctct ggtccctcaa gagagactaa
     4261 aaatcctttt gacatgtcta actcatttct tttttactac ttctattcta tcaaaaaaat
     4321 aggggtttgt gacaatcttt ttgtcttctc atttcatact caatgaaaat caaagagcaa
     4381 actaggaagc tagccgcagg ctgtacttga gtacggcaag gtgaagctga cgcagtttga
     4441 atttgatttt cgaagagtat cagccaccat aaaagaggca gataaaacta cctcttattt
     4501 acctagttct tgtgttcgtt tataggcttg atgaactgcc tccatgactg tgcctcgaaa
     4561 agcatgcgct tctaggcttg ctacgccagc aatagtcgaa ccgcctggac tacaaacttg
     4621 atctttcaaa actccaggat gttgctggct ttctaggacc aattgcccag ctcctaccac
     4681 agtttgggct gccattttca aggctgtttc tcgtggtaat cccgtctgaa cacctgcatc
     4741 tgccaaggcc tcaataaaga gatagacaaa ggctggtcca caacctgcaa gacctgtcgc
     4801 tgcattgatt aagctttctc ctaattcaac caagagacca gccttggcta aaagctgaca
     4861 aaagatctca ctgtcctcag ccctgcaatt aggagacaag gcataactaa tcactccttg
     4921 cccgatagaa gcaggggtat taggcatcat acgaataatt cggtgttggc ttgggataag
     4981 acttgctagt ttttctaagg tcaatccagc tgccatcgaa atcaaaagaa gactttctct
     5041 tttttcaagg atggtctggt attgagaaag cagttcagaa aactgagcag gcttaacacc
     5101 tagaaaaatc acatctgctt cttcgaatat ttcttcattg ttagaagtct gaccaccgaa
     5161 gttagcgatg aaagcatcca ccttgacttg actacgattg gcaaggagaa tctcatctga
     5221 cggtctagcc tgcaagacag acttggttaa actagcaccc atattcccca aaccgataaa
     5281 tccaatcttc atctcttact cccttatctg tccatcacca gtgaccacat acttgtagct
     5341 agtcaattcc ttcaagccca taggaccacg cgcgtgcaat ttctgagtag aaatccccat
     5401 ttcacaacca agaccaaatt gtcctccatc tgtgaaacga gttgaggcat taacatacac
     5461 cgctgcagag tccacttgat ctgtaaagta tgctgcagct tcagcatttt ccgtcacaat
     5521 ggcatccgaa tgatgggtac tgtgggattc aatatgcgca accgcttctt ctaaactgct
     5581 cacaacctta acagctagaa tatagtctaa aaactcggta tcaaagtctt gtgcttgagc
     5641 agcttgacct gaaacaaact ggcttgcttt gctatctagg cggaattgaa ttggttccaa
     5701 cccagcttct tttcgatctg caaccaacac ttgctccaag cgaggaagga agcttactgc
     5761 tttgttttca tgaaccagca gaacctccat ggcattacaa acagaaggac gactggtttt
     5821 agcattgttg atgatagaca gcgccttgtc ttcgtctgca tccttatcca catagacatg
     5881 gacaatccca gttcctgtct cgataacagg tacaatcgca ttctcaacca ctgcattgat
     5941 caagccagct cctccacgag gaatgagaag gtctagatag cccttggcct tcatcatagc
     6001 ataactactt tcacggctag tatcctccac cagttgaatc acatttggat gaatagtagt
     6061 cgtctccaag cccttcttca aggctgtgac aatggcatgg gttgtttgat aggcatcctt
     6121 accactacga agaacaaccg catttccact cttaagagtc aaagcagccg catcagacgt
     6181 cacatttgga cggctttcat agataatacc gatgacaccc atagctacac gtttttttgt
     6241 gataaccaaa ccattttcaa gctgacttgt ttctaaaact tcaccgattg gatctggtaa
     6301 ggcaaccact tcacgaattc ctcttgccat cgcttctata cgatctgcat ccaaataaag
     6361 acgatccaac atcacatctg agattttccc cttagccgct gccatatcga gggcattagc
     6421 cgctaaaatt tcctcagtag cagccactaa gtgatcagcc atggctagca aggcttggtt
     6481 tttcacttct tcactagctg tgttaatcga ttttttaaca gcctgtacct gttcaaattg
     6541 ttcttgtcta ctcaccatag tttacctcta aaattctgta aaaagtagtt ggatttcagg
     6601 agtaatggaa atccagtcgt cacggtaaat caagacaccc ttggcttttt gaaaacgcaa
     6661 catatcctcc aaagcagatg ctccaaattg cacgcgtcct tttccaagtg attttccact
     6721 ttccttatca aatactgtca cgatatcacc gtaagaaaag actccttctg cttcaacgat
     6781 accagataag agaagactct ttccatgttg agagagagct tccgcagccc ctttatcaac
     6841 ccaaatagaa ccttgactct gagcatagaa ggcaagccat tgtttctggg tacgaagccc
     6901 cttctcttga gcaacaaagt aagaaccatc ctcggtctcc tctgccgcct caaccatgga
     6961 atctgatttc aaggatgagc agatataaac aggaactcct gattccgtcg cgatagttgc
     7021 agccttgatt ttggttaaca tacccccagt tccgtttgac gaaccagctc caccagccat
     7081 atcaataatc tcacgattga tggtctcgat tctctccaag cgtttggctc ttggatctga
     7141 attaggattt ccagtataga gaccgtccac atctgtcaag aaaactaaaa ggtctgcttg
     7201 gaccatcgcc gctacttgag cacttagagt gtcattgtcc ccaaccttga gctcatcaat
     7261 aacgacacta tcattctcat tgatgatagg aattgcccca cggttgagca aaaccgacaa
     7321 agcctgatgg gcatttttat aacgacgctt atccacaaag tcatcttggg tcagcaagat
     7381 ttgtgcagaa acgatttgac gcaagagaag attggtcgta tattcttcca acaaaagtcc
     7441 ctgccctacc gctgctgaag cctgtttatc agcaatttta gtcggacgct ttttaaatcc
     7501 taaggctcca aaaccagccg caatggcacc tgaagacacc aaaatcaact catgaccagc
     7561 ctcgtgcagc atagccaact gctgggtaat atcctttacc ttactacgtg ataaacttcc
     7621 atcctcattt gtcagagaag aagtgcccac cttaaagaca atccgtttgt atttcatagt
     7681 ctcactccga ttattcatat ttatccatta taacatgaat aacaggaaat agaaaagagt
     7741 tgataggaaa aagaaggccc taaaaccttc ttttctacta ctattctgat tccgatttgt
     7801 ttacactcgt ttcagagctt tgtgaagttg aggatgtcaa atcttgacta gttgaagaat
     7861 ttgtaacact ttcatgttga ctcgtttctt tattttcaag atcctttttc actacttctt
     7921 tggattcttt tatttctgaa tgacttgtag ttttaattgt agcagatttc tcgatcggaa
     7981 catccactaa ccaggcacca cttgaatcca cagtatagcc ttctggtgtc ttaccattca
     8041 caagtaattg accattttct ttcaagaagt accatttatc cttgtcttta atccaaccta
     8101 tagctcgtga accacctgtc ttgtagaaat accaatcatt tgccttttta agccagcctt
     8161 gcttcatagc tcctgattct tctagatagt aatgatgtcc agcaacttct atctcacctg
     8221 tcttcatgat accagtagaa tccatatagt accaggcttc tttatctttt acccagcccg
     8281 ttttcatttc ccctgatgga gcaaagtaat accattgccc cttatattga agccaacctg
     8341 tcttcataga gccatcaaca tccaaataat accactgctc tttatcttga acccaaccaa
     8401 tttccatctg attttctttg ttaaaataat accagattcc atcaattttc ttccaatttt
     8461 tagcagaagc accagagtct gtcaaataga accaatgatt gttccatttt ttccattgat
     8521 tctgtaacaa gatacccgtt tggttaaagt aataccattc accttcgatt tcattccaac
     8581 cgacagcata ctctcctgta gaatcaggcg cttgatacca ccagttccca tatgcactct
     8641 tatgccaacc agcttgaaaa cttggaatcg gcttgtagga tgttgaaata ttgacaaaac
     8701 cgtcttttcg aatatcaaaa actgttgcat catagtcttt gctggctgcg ttgattctct
     8761 caattcctcg ttctttgagc caattaacat actcactatc aacaccattt ttccaaggta
     8821 gactatccga agtttgaaca atcaaactcg gactcaaatt tttaatgaaa tccttggtat
     8881 ttgatttgtt ggtatcatgg tgatgattaa acttcatcaa atcaactttt ccaatgagag
     8941 gaccatactt gtcttctgct ccatgaacat tatctaaatc gcccccaagg taaattttct
     9001 tgccattgac tttcaccacg ctaatcaagg aattggaatt gtcatcccaa attttcttta
     9061 attcacccga tgaatcagtt tcattttcat aattatagag ctgaatatcc atgtccccaa
     9121 actgaaaatg agcatcccct tgtgtgatat tttgaataac tgaaacacct ttttctgcag
     9181 cagtctgtaa aaccttatca tagccataca gattatccca tagacgttca gaattagtaa
     9241 tacgactatc actatatttc ttaagataga ctcggtcaac tggataggta gacagtaatt
     9301 catcaacatt tccaatatga tcactgtggg tatgggtcac caaaataaaa tcaagttttt
     9361 ggacacccaa ttccttcaaa cgacgaaaga cacggtctgt tagaacatgc ttataagacg
     9421 tttcaattcc ttctctccat ggataacgag aatcacttcc atctgggaaa tcataatctt
     9481 ctcctgtatc caccatggca aaatgtccat tgctttcaag aataatcgca tcactgccac
     9541 cttcttgaac attgataaag tggattttat ttcctgaact ttcttgagcc tgaacattcc
     9601 cataccatga caaacctaaa aaagcgccta caagtgctaa actagttaat ttctttttca
     9661 ttcttacttt ctcaatctct ccaaggtttc cttaaaaatc tctgggatat ctgctttaaa
     9721 ttccaaggtc ttacctgttc tcggatgagt aaaacctaaa gtcttggcat gaagaaattg
     9781 tccatgtcct ttcaaagtct tgcgaggacc atagacctca tcaccagcga ctggatggcc
     9841 gatataagcc atgtggacac ggatttgatg agtgcgccct gtctccagtt gcaactctac
     9901 taagctataa tcgccaaagc gttccaagac gtgaaaacgc gtcactgcag gcttcccttt
     9961 agcagttaca gcctgtttct tacggtcttt ttcactccgg ccaatcggcg cttcaattac
    10021 accacgatca ttaggcagat ttccatgaac aatcgcccaa tatttgcgga gagacttttt
    10081 atctttgagt tcttgggcaa gtactagatg cgcatcatcg tttttagcaa tcatgagaag
    10141 acctgacgta tccttatcaa tacggtgaac aatccctgga cgcagaaccc cattgatacc
    10201 cgacaagtcc ttaatatgat acatgagggc atttactagg gttccactgg tatgaccagc
    10261 actcgggtgc acaaccattc cctgaggttt gttaacgaca gccacatcct catcttggta
    10321 gactatttct agcggaagat cctcagccac atactctaat acctctggtt ctggcacatg
    10381 gtaagtgacg acatcaccct cttggactgt gtatttagct ttcttgactt gaccattgac
    10441 caagacctgg cctgatttaa tttgttcatt cgcgagacta cgtgataatt ctgacaaatc
    10501 tgacaaagcc ttatccaaac gcagaccacc agtttcaatt ttaatttcca tttatttcct
    10561 cttttagcat tgcaatcaat aaaataatca ctccaaccgt cagatagcta tctgccacat
    10621 tgaaaattgc aaagttgata aagtcaaggt ggaacatatc cacaacaaag ccctgactga
    10681 ccctgtcaat aaagtttcca agaccacccg cgattattag agtcaaaccc aagaacatcc
    10741 agaatgagtc ctccatgtgt ttatgtaaat accaaatggc acctatcacg acaaccagag
    10801 taatgacagc gaataacagc tgctgatctt gtaagataga aaaggctgca cctcgatttt
    10861 gcaggtaggt caagctaacg aaattgggaa tccaggagcg cacttcaccc agtggaatct
    10921 gctggacgat ataggatttg accaactgat ccagcccaat taaaagcagt acaatgactg
    10981 ccactattgc tctttttttc atgatttcct cttttgatca aaatattctt gcatgacttc
    11041 tacgaagaga gtcccagctt gactaagctc cacttcttca cgtttaacat agaccatgcg
    11101 gttatctagg ttatccttga gacgaataac tgtaatgcca ttaacactgt cactatctaa
    11161 aaatccagaa cctgtcgcat aggcgtccgt ccgctccaaa ataccattca aggtggcacg
    11221 gtctgtcaca ttaaacatct gtgagctagc gctggtatcg acaaagttct ctgaataata
    11281 aaggtactcg tctttctctt gagtgaaacg aaccgttggt aaatccgcta aatcctccat
    11341 gactaattcc tctttctggg ctaaaggatg accctcacag agataaatat gggtatggaa
    11401 aggaatcaat tcgatgacct ccagacctaa cttttcaacc cgttgcataa tccccttttt
    11461 attttgattg ttgaggtaga taatcccaat ctcactatgc ccttgcgcca cttcatctaa
    11521 tatttgaaca gtagttgatt caaaaatacg gaagttctta tagtcaggat agcgctctga
    11581 aaaggccgta atagttggtg gtaagaagtc atagtgctgg ctagcaacgg aaaattcatc
    11641 tttttcttct tcaggattgg catactgatt ttgaaaaata tcaaatcctt taaccaattc
    11701 ttgcgctttt tcataaaatt ccatcccacg acgggtcaag aaagtccctg agctggtccg
    11761 acggaaaatc ttaaagccca actctttttc caaatcacga acagaaatag acagactcgg
    11821 ctgactaaca tacatctttt cagcagcttc acgaaaagta ccactattgg caatagccac
    11881 aacatagcgt aattgttgaa tgttcatctt ctacccccaa cttctttatc ttttcattat
    11941 accatatttt agaagttttc caaaaaggaa aaaagaacat cctattcttc ttaactatct
    12001 tcactatctg cctttctcac gccaatctta ttttcaaaat caattcaaaa aaataagtgg
    12061 ctacacacca cttcagtatc ggaaagaaaa acgttgacta tttgtgaaaa aagaaaatgc
    12121 cggaaaattc cgacattttt ttagtaagct aacttcctga aaataggagt aaccaaacat
    12181 cccagaaggc agtgatattg atgagaatat gaactagtat tggatagtag agatttttag
    12241 tcatgcgata caatagagct aaaatcagac ctccactagc ataaatgaaa aaagcaagag
    12301 gagttaaagc aaaattgata tgaatataac cgaaaataat agcagaaagc aaaacatctc
    12361 cgtaccaagg tgagtttttg aaaaaggttg tcataagcac acctcgataa atcaattcct
    12421 cagcaatagg ggcgatgaag caaacgatga gcaagaaata agggaactcc tgtctcccca
    12481 tcatttctac cgtttcattc aaagaaatct gatttgaaga cagggatatg aaatacgaaa
    12541 agaggaagtc agacatatat gaaatgatgt agcccagtaa aaggtagatg aagtacctca
    12601 gctgccactt gaaatgaaaa attttctttc ctgcaaaaac cagtagatag atgaccgcta
    12661 agaaaagaac tccgctctcc atcaaaagca gaatctgaaa aaattcacga cttgctggta
    12721 gatagggctt tgcgaggtga ttgaaaactc tccaaaacca agttgatttg taaaaaatta
    12781 aggacaatgc tagtaaaatt tgaatagccc gttttttcat attaaattct ctgctttctc
    12841 cacttatttc ttcatattac taataaattt tacttaaaat ctcgcagcac ttcctttgca
    12901 aagatgattg cctcctccaa tatatcctaa atcataggtg cctctgggac aaaatccatg
    12961 aaccggcaga gttaatactg gttctatttc ccaatcgtac ctaccattag gatttttacc
    13021 atatgaatct gctccacctt gtatcagctc taattcttca tttgttagat ttatattttt
    13081 tgtcattaaa atcatgatat atttcctcca ttattcttca gattagttga gtaatctgat
    13141 actcatacct acttacaaaa aaactattat attaagttgg ttttttaatt tgtctagttg
    13201 caacattgac aaacatagta tagcatatct ttgcgaaata tcctcttcaa atcatgaatt
    13261 gtcatcaaaa catcttaaac tataaaatca attagtctca agctttctat caatttcttc
    13321 tccaaaatat gctataataa tagcaaaaga taaagaagga agacctatga ttaaactact
    13381 agccttggat atggacggca ccctcctcaa tgaagccaag gaaatcccac aagctcacat
    13441 tactgctatt cacaaagcca ttgaaaaagg tgtcaaactg gttctctgta cgggtcgccc
    13501 ccttttcggt gtcctcccct actacaaaaa actgggactc gacctccaga atgagtatgt
    13561 gattgttaac aacggttgtt caactcacca gactagcgac tggggcttgg ttgactggca
    13621 agaacttagt ccagctgaca tcgaatacct ctatgacctt gctgaaaaga gtgatgttca
    13681 gttgacactt tttgacgagt cacattattt tgttctcggt ggaaagccca atcaagttat
    13741 tgaaaatgat gctaaactag tattttcaga cctgactgaa atttctcttg aggaagcgac
    13801 tagtggaaag ttccggatgt tccaaggtat gtttttagga acaaaagaac aaacagacga
    13861 ttttgagcag cgttttgctg aagagctttg ccaacgattc agtggagttc gttcgcagcc
    13921 tgtcatttat gaagcaatgc cacttggaac gacaaaggct actgctcttt cacgactagc
    13981 tgagattttg aagattgatt cctcagagat tatggccatg ggcgatgcta ataacgatat
    14041 cgaaatgctc cagtttgcag ggcttgggat tgcaatggga aatgccagcg attatgtcaa
    14101 atctcttgcg gatgccgtta cctcaagcaa cgaagaagac ggcgttgcgc gtgctattga
    14161 gaaatatatt ctataaaaaa gaaaagcaaa tagacagaag ttactgctat ttgctttttt
    14221 gctaatattt taaaatacgg actaatcaat agagaagaat agcgaatcat aaaaatcgat
    14281 ttactagatg ccatagtatc ttatagttgc taataagaag ttagtctagc agaatgaatc
    14341 tctcatttct cccacactaa tctgtaattt gtctatacat ttctggtctt ctatctcgaa
    14401 acaagcccca gtttaggcgt tcacttgctc ccttgtctag gttataagta gctaacagaa
    14461 cagcttcttc ttgtctttca gctcgttcta gaatagctcc tgtttcatcc gtcataaagg
    14521 aggaaccgta gaagtcaaga ctggaactct gtccgccatt ttcctcacta ggagtaacct
    14581 cctctaaacc ataacgattg gctgcgatga ctggaacaat attcgctgct gcgtgccctt
    14641 gcatagtacg ttgccagtga ccacaactat ctgtatccaa aattggctct gaaccgatag
    14701 ctgtaggata aaagagcaat tcagcaccat tcaatgcaag acagcgcgct gtttcaggga
    14761 accattgatc ccaacagata ccgataccaa tcttagcata gcgagtattc cagaccttga
    14821 aaccagtgtt accaggcgtg aaatagaatt tttcttgata ataatggtca tctggtatat
    14881 gggtctttcg ataaacgccc agcacttccc catctgcatc aatgacggca atagagttat
    14941 acaagacatt accatctttt tcatagaaac tgattggtaa aacaacttgt agttccttag
    15001 caagcaactt aaaatgctga atggcagtat tttccgctac agattgggca tactggtagt
    15061 agtcatactg acgttcctga cagaaatagg gatgttcaaa caactcgggc aagagaataa
    15121 tttgggctcc ttgctcagca gcctgacgta ctaaacgctc tgcggtttgg atatttgttg
    15181 ccacatcctt agcgcattgc atctgaatgg ttgcaactct tacatttctc atctttttct
    15241 cctattctgg aatttgttgg gtgatacagt ggatattgcc accacctaag agaatatctc
    15301 tggctggtat tccgacaact ttacggtctg ggaaacactt gctgaggata tctaaggcca
    15361 cttggtcgtt tacatcctca aactgtggaa ccaagacagc cttgttggcg atataaaagt
    15421 ttacgtagga agctgctagt cgttcacctg cgtatcgctc ttcttctcct tcttcataga
    15481 tgtagcctgg caaatcttct tctgtcacaa cttgtcgaac tgcagggata ggcaatttat
    15541 gaatggtgaa gtgacaacct tttgcatctg tttcctgttc taagagttcg agatctgctt
    15601 ttgacatggc atactgggga tcattttcgt catctgtcca agccaaaaca acctcagcag
    15661 gaccaacaaa ggcaacaaca ttatcgacgt gttcattggt ttcatcctga taaataccat
    15721 aaggaagcca aataactttt tcagcaccaa gactttctaa taatgtgttt tcaatctcct
    15781 ctttagtcaa gttaggattg cgaccaggac taagcaagca actttcagtt acgagaatag
    15841 ttccttgacc atcgctatgg attgcgcctc cttccagtac aaaaggttta gcatcataga
    15901 caggcctttc caaggcctca gcaaaacgac tggctacttg gtcatcctct tcataatctt
    15961 gataaagacc atcataggtg cctccccaag cattaaaggc ccaatccacg gctaatttct
    16021 tacctttatc attgacgaga atggttgggc cagtatcacg cgcccaggca tcattggtgg
    16081 gaatgtctaa ataaacaacc ttgtctccaa gataggattg ggcttcagat agataggcct
    16141 gctccaccaa aagatagact ctttcccctt ctgctatggt ctcgatgatc tgagtaaatg
    16201 ctcttttagc agcctttcct tgaaaaggcc atgatcctgg tcgagtcggc catatcatga
    16261 gggtaccatg atggggttcg tactctgctg gcatgtgata gcctaatttt tttggactgt
    16321 ccatcatgat aatctccctt taaagtcttg atagccaaaa gctttgagta agctacagtc
    16381 tccctgttcg tccatgagat agagacttgg caatccaata ccattaaagg tattattttt
    16441 gacaaaagaa taaatggcca tgtcttgaaa ataaagtctg tctccgattt ggactggatt
    16501 ttcaaaacta taatcaccaa tcacatcgcc agtcagacag gtattagaag aaagtctgta
    16561 ggtatgggct ttttcctgtg actcaaagcc atttctcaaa ggtggacgat agggcatctc
    16621 aagtacatca ggcatatggc aggtcgcaga ggcgtctaaa accaagattt ccataccgtt
    16681 ttctacaata tctaatacct cagttgctaa ataacccgca ttaagcgcaa tggcttcacc
    16741 aggctcgata tagatttcaa gattgtaagt ttttcggata cgcttgattt ctgaaatcag
    16801 caaatccaca tcgtaacctt ctcttgtaat atgatgacca ccacccatat tgagccattt
    16861 tacctcatgt aagtagggac caaactgttc ttctactgct ttcaaagttg tttgtaaatc
    16921 atctgctccc tgctcgcaaa gggtatgaaa atgaagtccg tcaaccaaat ctagcaaatc
    16981 gctcggaatc ttgtctatag taactccaaa gcgagaacca ggtgcacaag ggtcatagag
    17041 cgcgtgatct ccttgagttg aacactgagg gttgaggcgc aaaccaacac tgacaccagc
    17101 ctctcgacaa cgagccccat gcttacgcaa ctgtctctct gagttaaaga cgatatggtc
    17161 tgttatccca agcaattcct ccatgtcctc atccttgaaa gctggtgcaa aaacatggac
    17221 ttcaccagga aattcttccc ttgccaattt ggcctcatag agtccactag ctgtcgtacc
    17281 tgatagatac tggctaatca agggataagt tttgtagagg gaatatgcct tctgggcaag
    17341 caagaccttg caaccggcct cttcttgtac atattgtaga atgcggcaat tagcttctaa
    17401 cttggccaag tcaataacat aggctggtgt tggtacttgt tctaacttca ttagtccaac
    17461 atttgtggat tttcaaccac aacccatggc aaaccatact cattcaaagc ttccatgaat
    17521 ggatctggat ctaactcctc aaggttatac actccagctt gtttccaagt tccgttcatg
    17581 actaattttg tcccaatcat ggctggaacg cctgtcgtgt atgaaattgc ttgtgacccg
    17641 acttctgcat agcactcttg atggtcgcaa acattgtaga tgtagatagt cttttcaacg
    17701 ccatctttga cacctgtaaa gatacatcca atattggttt tacctacagt acgtggacca
    17761 aggctagcag gatctggaag caaggctttc aaaaattgaa ttggaacaat ttcttggccg
    17821 ttaaagttaa tggtatccgt acgaaggagt ccaacatttt caagacattt catgtgcgtc
    17881 aagtaagatt gaccaaaagt cataaagaaa cgaatgcgtt tgacacctgg aatgttcttg
    17941 gccaatgatt cgatttcttc atggtgaagg agatacatat ctttttgtcc aacttgaggg
    18001 aaatcatact cacgcttgat agacatagct tcgacttcga cccatttccc atcttcccag
    18061 taagaacctg gcgcagaaac ctcacggaga ttaatttctg gattaaagtt ggttgcaaat
    18121 ggataaccgt ggtcaccgcc attacagtct aaaatgtcga tataatggat ttcatcaaaa
    18181 tagtgtttga gggcataagc tgaaaagaca ctagttacac ctgggtcaaa accagaacca
    18241 agaagagcag tcaagcctgc ttctttgaat ttctcttgat aagcccactg ccatgagtag
    18301 tcaaagtagg ctgtaaaacc aagttcctta caacgttttt cgtagatagc acgccactca
    18361 gggtcttctg tgtcttctgc ttcgtagttg gctgtatcga tatagtgaac acctgttgcc
    18421 aaacaagcat ccataatggt taaatcttga taaggcagag ctacattcaa aacagcttct
    18481 ggtttgtagc tttcaatcag agcaatcact tcttcaacct tgtcagcatc aagagcagct
    18541 gtctcaatct ttgtacttgt tttgccttcc agtttagctt tcaagtcatc gcattttgac
    18601 ttggtacggc tagcaatcat aatctctgta aatgtttcgc tatcttgaca aatctttgaa
    18661 atagcaactt gggcaacgcc cccacaacca ataactagta aacgactcat ttttttcctt
    18721 cctcttcttc taaaatgtcc tcaacatact tgggcaacat aaaggctccc acgtgtaagt
    18781 ttgcagtgta gtattctgtg aaaagctggc gttttttcca gccttccttg tcaaaatctt
    18841 tgacagggtg gtattttttc gatgcaaatc caaacaacca atagccagct gggctagttg
    18901 gaatatgggc ctgataaacc cgactgattg gaaaggcttg attgaccttg cggtgcatgc
    18961 ttcggcaggc cgactcatcc tcgtcaaaga acggactccc atgctggtaa atcatgatgc
    19021 cgtcttcctt cagagctcga taactattgc cgtagaattc cttggtaaag agtccttccg
    19081 tatggccaaa tggatctgtc gcatcgttga tgataatatc atagtcatat tcgcagtttc
    19141 gcaaaaagcg tagcccattt tggtagtaaa tggtaacacg aggatcatct aggcctgcag
    19201 caaagtctgg gaaatactca cgacagacct cgaccaacat ctcatccggt tccacgatat
    19261 cgatttgttc cagttcagga tagagtgtta atacttgggc aacaccgccg tcaccacccc
    19321 caataaccaa tactttcttt ggatttgggt ggacagccat gggaacgtga acggtcattt
    19381 cattgtagac gaaatcatcc gcatctgaga acaagacatg gccatttaaa atcagtattt
    19441 tcccaaaagc tggcgtatcc aagacttcga tatcctgcca ttcacttttt ccagcgtaaa
    19501 gttgcttggc tgttctcaga gacaatttga catctggagt atgaacttca gaaaaccata
    19561 aatccatcta tttctcctta tattata
//
LOCUS       DAVBBS010000045        18759 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVBBS010000045 DAVBBS010000000
VERSION     DAVBBS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18759
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.045"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..338
                     /locus_tag="VQU32_001513"
     CDS             <1..338
                     /locus_tag="VQU32_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET2472948.1"
                     /translation="CLSETADKNWHKSDRIFVTNTGKTVHSSILSKSLQRANERLKKP
                     IPKHLSPHIFIHITISILSENKIPLKTITDRVGHPDSEVTNSIYTHVTKNMKDEAINV
                     LDKVMKKIF"
     gene            complement(830..1240)
                     /locus_tag="VQU32_001514"
     CDS             complement(830..1240)
                     /locus_tag="VQU32_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472949.1"
                     /translation="MPKKKIERISVIHREKILWLKWYFMRDKEKPKYSVLECKMFDAA
                     KNKDMLAYKKYATIKQITDIRVQTSEDDILTAIKEVYVYNHMNVIGACQRILFVSQSP
                     AYNKLNKWFETYSDLYFSIIPLPNMGAYHELVDS"
     gene            complement(1258..1635)
                     /locus_tag="VQU32_001515"
     CDS             complement(1258..1635)
                     /locus_tag="VQU32_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472950.1"
                     /translation="MRKYLKEIKELQELKELLSSRNTPEVIIVEGNDDLGEFFQVDGE
                     LFSDIELLENLKKWREWEVQVIVDDWCNRGLNEYETGILYFPKHEDKMDYIRFNKGLE
                     PLYHALDEPYTTISKSEWLKLLD"
     gene            complement(1577..2005)
                     /locus_tag="VQU32_001516"
     CDS             complement(1577..2005)
                     /locus_tag="VQU32_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HET2472951.1"
                     /translation="MAGNENDGLTSKQIKFIDAMLTEPTIDKACQKAGVSRATGHKYL
                     KVAAVKKTLRLKQDEMMDKTTQMLYLASSNAVSVLNDIMMDAKINPFIRTQAAKTILE
                     QSYKTHEIFGVVRQIEELRLEIEEVSKGDQRVTRTQGIIK"
     gene            complement(2049..2654)
                     /locus_tag="VQU32_001517"
     CDS             complement(2049..2654)
                     /locus_tag="VQU32_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472952.1"
                     /translation="MTFTTIKDDIKAFGKKKHGYMTGYIAKQEELHKQLQNGMIGEKY
                     AKDQLEAYKLEGDTYSRDTYNKIHAEIEKQHDLELEALKEKELSVTADDVAELTLLAS
                     MKMTKDELLGYFEKYKNKPLAIKKLWSIAEQYPEIAINLELFNAEQALESLILFFKRQ
                     LSYCHYSLLINGDKIQAVTTEMVVNSDAPELDRRLDEYLNK"
     gene            complement(2744..2917)
                     /locus_tag="VQU32_001518"
     CDS             complement(2744..2917)
                     /locus_tag="VQU32_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472953.1"
                     /translation="MTENKDNKLLEMMEKGFVLYSKNGIIKYIEIPDHGSIKLKAQDG
                     QLVYKEVTTGEQC"
     gene            complement(3516..4412)
                     /locus_tag="VQU32_001519"
     CDS             complement(3516..4412)
                     /locus_tag="VQU32_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET2472954.1"
                     /translation="MKSLQSSLGNIETFKLDELCPKHEIQLTQIKGERHVVVGWNENG
                     EVIKEVRCIPPYCEQCQEEQKKQDEEDAIADNLNAKLYLQTYNVLMSNKSYVADEFKT
                     KTFDDFNAVTSEEKRFLEFAKGQVQKYLDGMRGNTLITGGTGIGKTLLSVAIAKGINE
                     GYKTKGEPKSVLFVSLTEMIKQIKEGWNYGKGATLTEFEATELMKSVDYLIIDDLGAK
                     NAVIKPKSDWEQDLLFDVLNNRENTIFNTNLDSDELKIVYNERNYSRILKGLEGNTFK
                     AFTIKDKRYSINQLKNKVKQLK"
     gene            complement(4424..5233)
                     /locus_tag="VQU32_001520"
     CDS             complement(4424..5233)
                     /locus_tag="VQU32_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HET2472955.1"
                     /translation="MAKTKVYFWLKIDKKFFDNIFIKRLKTVPGGYTMTVIYIRLMLE
                     SLESDCILYYEGYFNNLKEELALKLDVSEDDIDMTMAYFTKCGLIQIDEDKNAELPQA
                     KAMVMSETNWASYKREQRKKGKLEEVQPSLTFSNSCPTEIEKELEIEVDKEQSPTPST
                     INQDFVNLYKSFEAETGKALSPLQIQELQYMLEDFSPELIHEALKEAVSQGKANFAYI
                     KAILNRWKQDNLLTVELVRNSRAAREAKKQQTNQLEPTSYEDWIPTQEKPF"
     gene            complement(5308..5523)
                     /locus_tag="VQU32_001521"
     CDS             complement(5308..5523)
                     /locus_tag="VQU32_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472956.1"
                     /translation="MNELDISNTQAVFVTVILIGLLLYLNHRDRKKSAQLERENQQTI
                     ETPSEDLNPDYGRYIQLAGVDVWGRIE"
     gene            complement(5525..5725)
                     /locus_tag="VQU32_001522"
     CDS             complement(5525..5725)
                     /locus_tag="VQU32_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472957.1"
                     /translation="MTLDLDNMTQAEFDEAMAEIQLQSPNIFQLISDFVNKKVTSVEI
                     DDLLNMKRAEQVAYIKNYKARA"
     gene            complement(5722..5913)
                     /locus_tag="VQU32_001523"
                     /pseudo
     CDS             complement(5722..5913)
                     /locus_tag="VQU32_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841301.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5902..6192)
                     /locus_tag="VQU32_001524"
                     /pseudo
     CDS             complement(5902..6192)
                     /locus_tag="VQU32_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            complement(6362..6514)
                     /locus_tag="VQU32_001525"
     CDS             complement(6362..6514)
                     /locus_tag="VQU32_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472958.1"
                     /translation="MKIVTFKPTKQIDGGFYLPGIDILFVSDKADAKSTEDVILFISR
                     NGLNKS"
     gene            complement(6525..6791)
                     /locus_tag="VQU32_001526"
     CDS             complement(6525..6791)
                     /locus_tag="VQU32_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472959.1"
                     /translation="MEILSKEIQLQGLQLLKQTLETLVELEKQRSSKLDLISRKELMD
                     LLGISATTLDNWEDLALKRYQTPMDGAKKVFYRLSDVYLFLAIK"
     gene            complement(7002..7184)
                     /locus_tag="VQU32_001527"
     CDS             complement(7002..7184)
                     /locus_tag="VQU32_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2472960.1"
                     /translation="MLITLSLAEKVRIKRARLQLTKKAVSEQLGIKSQTLTKVENGNY
                     DASKRIYESVMTWLVE"
     gene            7246..7749
                     /locus_tag="VQU32_001528"
     CDS             7246..7749
                     /locus_tag="VQU32_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2472961.1"
                     /translation="MYKMNRIKQLRKENNLTQRELANETKIPYRTIQRWENGETDIKS
                     DAAQVLADYFGVSIPHLLGYSDLIADTDSDIKAMAYSHLLTFVDEKQIKEFEKEFIRA
                     NDNSDTFGDLGSGEKALEKYYQALCYLVPPFRDILARYSFLSLDEKKSVYNIIVGLSN
                     NLSKENK"
     gene            7823..8986
                     /locus_tag="VQU32_001529"
     CDS             7823..8986
                     /locus_tag="VQU32_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET2472962.1"
                     /translation="MNIIEKIKKNGQRVYYASVYLGVDQLTGKKARTTVTATTKKGVK
                     VKARDAINTFAANGYTVKDKPTITTYNELVKVWWDSYKNTVKPNTRQSMDGLVRVHLL
                     PVFGDYKLSKLTTPILQQQVNKWADKANKGEKGAFANYSLLHNMNKRILKYGVAIQVI
                     QYNPANDVIVPRKQQKEKAAVKYLDNKELKQFLDYLDTLNQSNYENLFDVVLYKTLLA
                     TGCRISEALALEWSDIDLESGVISINKTLNRYQEINSPKSSAGYRDIPIDKATLLLLK
                     QYKNRQQIQSWKLGRSETVVFSVFTEKYAYACNLRKRLNKHFDAAGVTNVSFHGFRHT
                     HTTMMLYAQASPKDVQYRLGHSNLMITENTYWHTNQENAKKAVSNYETAISNL"
     gene            complement(9140..10444)
                     /gene="eno"
                     /locus_tag="VQU32_001530"
     CDS             complement(9140..10444)
                     /gene="eno"
                     /locus_tag="VQU32_001530"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET2472963.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            10608..11054
                     /locus_tag="VQU32_001531"
     CDS             10608..11054
                     /locus_tag="VQU32_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HET2472964.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(11051..12166)
                     /locus_tag="VQU32_001532"
     CDS             complement(11051..12166)
                     /locus_tag="VQU32_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET2472965.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIDVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGRHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(12175..12504)
                     /locus_tag="VQU32_001533"
     CDS             complement(12175..12504)
                     /locus_tag="VQU32_001533"
                     /inference="COORDINATES: protein motif:HMM:NF019887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HET2472966.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGGNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(12458..12820)
                     /locus_tag="VQU32_001534"
     CDS             complement(12458..12820)
                     /locus_tag="VQU32_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HET2472967.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHC"
     gene            complement(12879..14312)
                     /gene="glgA"
                     /locus_tag="VQU32_001535"
     CDS             complement(12879..14312)
                     /gene="glgA"
                     /locus_tag="VQU32_001535"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HET2472968.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(14309..15448)
                     /gene="glgD"
                     /locus_tag="VQU32_001536"
     CDS             complement(14309..15448)
                     /gene="glgD"
                     /locus_tag="VQU32_001536"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HET2472969.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTFLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVRTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(15438..16580)
                     /locus_tag="VQU32_001537"
     CDS             complement(15438..16580)
                     /locus_tag="VQU32_001537"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET2472970.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(16570..18498)
                     /gene="glgB"
                     /locus_tag="VQU32_001538"
     CDS             complement(16570..18498)
                     /gene="glgB"
                     /locus_tag="VQU32_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET2472971.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEYGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAIVTELPDKKWRDGLWLARRKRWGFAERPVNIYEVHAGSWKRNPDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDIYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEVIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         5571 a         3929 c         3160 g         6099 t
ORIGIN      
        1 attgtctaag cgaaacagcc gataagaatt ggcacaaaag cgaccgtatt tttgttacca
       61 atacaggaaa aacagttcat agttctatct tgagcaagtc tctccagcga gcaaacgaac
      121 gccttaaaaa accaattccc aaacatctgt cccctcacat cttcatacac atcactatta
      181 gcatcttatc agaaaataaa attcctttaa aaacaatcac ggacagggtt ggtcatcccg
      241 actctgaagt cactaattcc atctacaccc acgtcacaaa gaacatgaaa gatgaagcaa
      301 tcaatgtact ggataaagtt atgaaaaaga ttttttaaaa agttttgtcc cttttttgcc
      361 ctctaaatac aaaaatagcc cttcggataa aatccgaggg gctagaaacg ttgttaaatc
      421 aacggccgaa cttttgaatt tcatggttcg ggataaaata gttcactgaa ctattttatt
      481 ttttaaggtt atcataatat caaatagttc aattaaatac gctaaattac taatatactt
      541 tttacctttt tcattctaaa atgtaaagta caaacaatta caatatacta gagggggagt
      601 aaaaaaggta ataaaattga cgagttcagc aggcaaggga aatagcactt ttcatggtgt
      661 tattttttta attcagcaat ggagtagccg cttaattgat agaaacaaaa agcacctagt
      721 ttcctagatg ctagcacaat agcccggatt cgaaccggaa tctcctcaat taaggtgcaa
      781 tccctatata ctactcattg tgctttggtt cattatatca tacaatcaac tagctatcta
      841 ccaactcatg atacgctccc atattcggta atggaataat actaaaatac aaatctgagt
      901 aagtttcaaa ccatttattc agcttgttat aggctggcga ctgactaaca aataatatcc
      961 gttgacaagc tccgataaca ttcatgtgat tatacacata gacctcttta atggccgtta
     1021 aaatatcgtc ttcacttgtc tgtaccctaa tatctgttat ctgcttaatc gtagcgtatt
     1081 ttttataagc tagcatatct ttatttttag cagcatcaaa cattttacac tcaagaacac
     1141 tatacttagg tttttcttta tctctcatga aataccactt gagccataaa attttttctc
     1201 tgtgaattac tgaaatacgt tcgatttttt tctttggcat gattacctcc taaattatta
     1261 atctaatagc tttaaccact cactttttga gattgttgta taaggttcat ctagcgcgtg
     1321 atataaaggt tctaaacctt tgttaaatcg gatatagtcc attttatcct catgctttgg
     1381 aaaatataag attcctgttt catattcatt tagaccacgg ttacaccaat catcaacgat
     1441 aacctgcact tcccattcac gccacttttt aaggttttct aaaagttcaa tatcactaaa
     1501 taactcacca tcaacttgga aaaattctcc cagatcatcg ttaccctcga cgatgataac
     1561 ttcaggcgta tttctactac ttaataattc cttgagttct tgtaactctt tgatctcctt
     1621 tagatacttc ctcaatttct aacctcaatt cttcaatttg tcttactact ccaaaaattt
     1681 catgagtttt atatgattgt tcaagtatag tttttgctgc ttgagttctt ataaaagggt
     1741 taattttggc atccatcata atatcattga gtacagaaac agcattggat gaggctagat
     1801 atagcatctg tgtagtttta tccatcatct catcttgttt tagtctcaat gtctttttaa
     1861 cagctgcaac ttttagatac ttatgccctg ttgcccttga cacccctgct ttttggcacg
     1921 ctttatctat cgttggctca gtaagcatgg catctatgaa ttttatttgt ttagatgtaa
     1981 ggccatcatt ttcatttcct gccatctatt acctcctcgt tatcaaaata aaggggttgc
     2041 tagcccctct atttgtttaa atactcatcc aaacgacggt caagctcagg ggcatctgaa
     2101 tttactacca tttcagttgt tacagcttgg attttatcgc cgttgataag caaactgtaa
     2161 tgacaatatg aaagttgccg cttaaagaat aagattagac tttctagcgc ctgctctgca
     2221 ttgaataatt ccaagttgat agcaatttct ggatattgtt cagcaattga ccaaagtttt
     2281 ttaatagcaa gaggtttgtt cttgtatttt tcaaaataac ctagcaattc atcctttgtc
     2341 atcttcatac tagctaacaa ggtcagctct gccacgtcat cagctgttac gctgagttct
     2401 ttttctttta atgcctctag ctctagatca tgctgttttt caatttctgc atgaatttta
     2461 ttataggtgt ccctagaata agtgtccccc tctaacttat aagcctctag ctggtcttta
     2521 gcgtattttt cgcctatcat accattttgt aactgtttat gtaattcctc ttgtttagca
     2581 atgtagccag tcatgtaccc atgctttttc ttgccaaaag ccttaatatc atccttgatt
     2641 gttgtaaatg tcatgttatc ctccttacga aaaaataaaa gaggtatgac aaaagagcgt
     2701 taaactcaat tatcatacct ctggtttttc cagtcagtat ttattaacat tgttctccag
     2761 tggtcacttc cttataaact aactgcccat cttgagcttt tagcttaatg ctaccatgat
     2821 ctggaatttc aatgtacttt attataccat tttttgagta taaaacaaag cctttctcca
     2881 tcatttctaa taatttatta tccttgtttt cagtcatgaa tttctccttt atttcaagtg
     2941 tcccaacggg tacggttgta agctgagaca ttttagggct atctttgagc gacaaaaatg
     3001 tcgaaaagtc ttcagttcct gtataaccgt tattagtttt gtttgctact catttttgag
     3061 tcacaaatct gttaagcaaa aatgagtaat agctcgtaag ataagtaaat ctaatacttg
     3121 tattcctaaa gcgttgatat aactgatatt ttcaactatc caacgctctt tactaccctt
     3181 tttagtgtgc tttagctcat ttttgagctt ttttgagctt ttttgagttc cattttggga
     3241 atgcaaagca ggcgcgcacc gtgcatgctt ttttcttgct catagcctca ccctcagaag
     3301 tttgccgacc gagagcgtgg ggctttttat ttttgtataa ttaaaaacag ctgcacaatt
     3361 tttgtacagc tacgaccgag gcaggggcga cctgccgtac tttacactag ataatatcat
     3421 ctaggtagtc caactgtatt ttatcatatt aaaggctaca ttacaatttc tcaccctgtg
     3481 aagagtgtct atttttgttt aggtttgtaa gtcgattact tcaactgctt taccttattt
     3541 tttaactgat taattgagta tcgcttatct ttgattgtga aagccttaaa agtgttaccc
     3601 tcaagacctt ttagaatacg gctataattc cgctcgttgt atactatctt gagttcatcg
     3661 ctatcaagat ttgtattaaa tatagtattt tctcgattat tcagtacatc aaaaagtaaa
     3721 tcctgctccc aatcgctctt aggtttaata acagcatttt ttgcacctaa atcatcaata
     3781 attaagtaat caacagactt catgagttca gtagcttcaa actctgtaag tgttgcacct
     3841 ttaccataat tccacccctc tttaatttgt ttgatcattt cggttaggct tacaaaaagc
     3901 acactcttag gttctccttt tgtcttatac ccctcattta tacctttggc aatagcaact
     3961 gataaaagtg tttttccaat ccctgtacct cctgtgataa gcgtatttcc cctcatgcca
     4021 tcaagatatt tttgtacctg accttttgca aattctaaaa atcgcttttc ttctgatgtt
     4081 acagcattaa aatcatcaaa agttttagtt ttaaactcat ctgctacata gctcttattg
     4141 ctcatcaaca cattataagt ttgcagatat agtttagcat tcaaattatc agcaatcgca
     4201 tcttcttcat cttgcttttt ctgttcttct tggcattgtt cacaataggg tgggatacag
     4261 cgaacttctt ttattacctc tccgttctca ttccacccca ctactacatg tctttctcct
     4321 ttgatttgtg ttagctgtat ttcatgctta ggacacaatt cgtctagttt aaatgtctca
     4381 atatttccta aactagattg taatgatttc attttctgac ctcctaaaat ggtttttctt
     4441 gtgttggtat ccaatcttca tagctggtag gctctagttg attggtttgc tgttttttag
     4501 cctcacgcgc tgccctgcta tttctaacaa gttccaccgt caataaattg tcctgtttcc
     4561 aacggttaag gattgccttg atgtatgcaa agtttgcttt accctgactg acagcctctt
     4621 ttaacgcctc atggataagc tctgggctaa aatcttctag catatactgc aattcttgaa
     4681 tctgtaacgg tgacaatgct ttacctgtct cagcttcaaa agatttatag agatttacaa
     4741 aatcttgatt gatggtagag ggagtgggtg attgttcttt atctacttct atctctaatt
     4801 ctttctctat ctctgttgga catgagttgg aaaatgtcaa agatggttgg acttcttcca
     4861 attttccttt ttttcgttgt tcacgtttat aactcgccca gtttgtttca ctcataacca
     4921 tagcttttgc ctgtggtagt tctgcgtttt tatcttcgtc aatctgtatt aaaccgcatt
     4981 ttgtaaagta tgccatggtc atatctatat catcttctga tacatctaat tttagggcta
     5041 actcttcctt gagattgtta aagtagccct catagtataa aatacagtca ctttccaagc
     5101 tctctaacat gagcctaata taaataactg tcatagtgta cccaccaggt accgtcttta
     5161 gtctcttgat aaaaatatta tcaaaaaatt tcttatcaat ttttagccaa aaatagactt
     5221 ttgtttttgc catcgtcctc tcccttgctc atttataatt ctctaccttg attagtctta
     5281 atactcgctt gtcattcatt gttagactca ttcaattctt ccccatacgt ctacccctgc
     5341 aagctggata tatcgcccat aatcagggtt taaatcctcg ctaggtgttt ctatcgtctg
     5401 ttggttttct cgctccagtt gggcgctttt tttgcggtct cggtggttta aataaagcag
     5461 taagccaatc aaaatcacgg taacaaaaac cgcctgtgta ttgcttatat ctagttcatt
     5521 catgttatgc cctcgctttg taattcttga tgtatgccac ttgttcagct cgcttcatat
     5581 tcaacaagtc gtctatttcc acactggtta cttttttatt tacaaaatca gaaatcagtt
     5641 gaaaaatgtt tggactttgt agctggattt cagccattgc ctcatcaaat tctgcttgtg
     5701 tcatgttgtc taaatctagt gtcattgcat tgcctcctca aacttctcta taagacaact
     5761 tttatttgct ttctgagttc catttttaga gttaaaaaga atatatttta aggttacagt
     5821 agcctctaaa tactcctttt cagcatgctc tatatacgcc tgttgctctg cttcgttctc
     5881 aaaaaagtgc ttagcttagc gtttaaagaa tgctttgact aggtttagtt tgaattgccg
     5941 tactgcttcg gtattcttta gataagtgat cagcaaagta gcctgttgct cgttcagaat
     6001 ataggatttt ttaggttgtc ctctagtgtc taatttatgg attttaaatc caagtattcc
     6061 caactcttca aagtcaacct tattttctct tattaagcgt gtgatagtgt ggtgttgtac
     6121 gtcagcacat tcagcgatga tctcgcttgt ggtatacggc tctttcttgc cgtccatgta
     6181 gactagttcc attatggttc tacctcctgt ataaatttag ctagcttact ttttaattgt
     6241 ctcctctagc cctttttagc ctctacaacg gttttggcta acgtgttaga ggcttttata
     6301 acggcctgta tagctgtctc tatttcctcc gtggaatagt agctttgttt tctaatatca
     6361 tttaagactt gtttaaccca tttcttgaaa taaataaaat tacatcttct gtactcttgg
     6421 catctgcttt atctgagaca aatagaatgt caatgcctgg tagataaaac ccaccgtcta
     6481 tttgtttagt tggtttgaaa gtaactattt tcatttcata actcctattt tattgctaaa
     6541 aataaataca catctgacag acgatagaat actttcttag ctccatccat cggggtctga
     6601 tatcgtttaa gagcaagatc ctcccagtta tcaagggttg tagcacttat acctagcaga
     6661 tccattaatt ctttacgaga aattaaatct aacttactag atcgttgttt ttctagctca
     6721 actaaagttt caagagtctg tttaagaagc tgtaaaccct gtaactgtat ttctttagac
     6781 agaatttcca tttatatcct ccattttcct ttttataaat taagttgtct ttttcctcta
     6841 attttgataa aataagaaga gattatttag caaagggggt tgtccctttg ctaaatacct
     6901 aacaaaacca cgcgccttgc ctttgcttga gttttgttat ttttccttag ccgtgtacgt
     6961 tggtttggtc gcctgtacat ggctttttac ttgcgttaaa tttattctac tagccaagtc
     7021 atcacgctct catagatacg cttagaggca tcatagtttc cattctcaac ttttgttagt
     7081 gtttgagatt taatacccaa ctgctccgat actgcttttt tggttagctg taatcttgca
     7141 cgcttgatac gtactttttc agcaagtgat agtgtaatca acattggttt cacctccctt
     7201 ttttctccat ttttggcatt ttgtgttata atctctttag aaaggatgta taagatgaat
     7261 agaattaagc aattacgaaa agaaaataat cttactcaaa gagaacttgc taatgagact
     7321 aaaatccctt atagaaccat acaacgttgg gagaatgggg aaactgatat aaaatccgat
     7381 gctgctcagg tacttgctga ctattttgga gtatcaattc ctcacttact aggttatagc
     7441 gatttgatag cagatacaga tagtgatatt aaagcaatgg catactcaca tctactaact
     7501 tttgtagatg aaaaacaaat caaagaattt gaaaaagagt tcattcgtgc aaacgataat
     7561 agtgatactt ttggtgattt aggaagtggt gaaaaggcgt tagaaaaata ctatcaagca
     7621 ttatgctatt tagttcctcc ttttagggat atattggcta ggtatagctt tttgtcacta
     7681 gatgaaaaaa aatcagtcta taatattatt gttggtttaa gtaacaactt atcaaaagaa
     7741 aataaataac tttaaacatc atgtataaat acctaacaaa accacgcgcc ttgcctgctg
     7801 atggaaagaa aggtacaaat acatgaatat catagagaaa attaaaaaga atggccaaag
     7861 agtttattat gctagtgttt atctaggcgt tgaccaacta acgggcaaaa aagcccgtac
     7921 aactgttaca gcaaccacta aaaagggcgt taaagtaaaa gcgcgtgatg cgatcaatac
     7981 ttttgctgct aatggctata cagttaaaga taagccgaca attacaacat acaatgagct
     8041 tgtaaaagtt tggtgggata gttacaagaa tacagttaag ccaaatactc gccaatccat
     8101 ggatggattg gttagagtgc atttattgcc tgtatttggc gattacaagc tatctaaact
     8161 tactacgcct attcttcaac agcaagtaaa caaatgggct gacaaggcaa ataaaggcga
     8221 aaaaggggca tttgctaact actccttgct ccataacatg aataagcgta ttttgaaata
     8281 tggcgtagct atccaggtaa tacaatacaa cccagctaat gatgtcatcg ttccacgcaa
     8341 acagcagaaa gaaaaggctg ctgtcaaata cttagacaac aaagaattaa aacagtttct
     8401 tgattattta gacactctga atcaatcaaa ttatgagaac ttatttgatg ttgtcctgta
     8461 taagacttta ttggccactg gttgccgtat tagtgaggct ctagctcttg aatggtctga
     8521 tattgaccta gaaagcggtg ttatcagcat caataagaca ctaaaccgct atcaggaaat
     8581 aaattcacct aaatcaagcg ctggttatcg tgatatacca atagacaaag ccacattact
     8641 tttactgaaa caatacaaaa accgtcaaca aattcagtct tggaaattag gccgatctga
     8701 aacagttgta ttctctgtat ttacggagaa atatgcttat gcttgtaact tacgcaaacg
     8761 cctaaataag cattttgatg ctgctggagt aactaacgta tcatttcatg gtttccgcca
     8821 tacacatact actatgatgc tctatgctca ggctagcccg aaagatgttc agtatagatt
     8881 aggccactct aatttaatga tcactgaaaa tacttactgg catactaacc aagagaatgc
     8941 aaaaaaagcc gtctcaaatt atgaaacagc tatcagcaat ttataaaaaa taagggtgac
     9001 ccatttccgg gctaccctct tactatacca aaaattagta ggggtagtaa aaagggtatt
     9061 aaattataaa aagcactaag ggaaagcgcc ccaaagtgct tatttcaaag gctttatagc
     9121 ctataatcac ataaggagat tattttttaa ggttgtagaa tgatttcaat ccacgatatt
     9181 cagctacttc accaagttgg tcttcgatac gaagcaattg gttgtattta gcgatgcggt
     9241 ctgtacgtga aagtgaacca gtcttgattt gtcctgcgtt agttgcaact gcaatatcag
     9301 cgattgttga atcttcagtt tcacctgaac ggtgtgatac aacagcagtg taaccagctt
     9361 ctttagccat ttcgatagct tcaaaagttt cagtaagagt accgatttgg ttaactttga
     9421 taaggattga gttagcagca ccttcttgga taccacgtgc aaggtagtca gtgtttgtta
     9481 cgaagaagtc gtcaccaaca agttgtactt tcttaccaag acgttcagta agagctttcc
     9541 aaccatccca gtcgttttca tccataccat cttcaatagt gatgattggg tatttgttaa
     9601 ccaattcttc aaggtagtcg atttgttctg cagatgtacg aacagcagca ccttcacctt
     9661 caaatttagt gtagtcgtaa actttacgtt ctttatcgta gaattctgat gaagcacagt
     9721 caaatccgat aaatacgtct ttacctggta catatccagc agcttcaatc gcagcaagga
     9781 tagtttcaac accatcttca gttccttcga aacgaggagc gaatccacct tcgtcaccta
     9841 cggcagtttc caaaccacgt gatttaagga ttttcttaag agcgtggaag atttcagcac
     9901 cgtaacgaag ggcttcttta aatgttggcg caccaactgg caagatcatg aactcttgga
     9961 aagcgattgg agcgtcagag tgagaaccac cgttgatgat gttcatcatt ggagttggaa
    10021 gaactttagt gttgaatcca ccaaggtagc tgtaaagtgg gatttcaagg tagtcagcag
    10081 cagcacgagc tacagcgata gacacaccga ggattgcatt cgcacccaat ttacctttgt
    10141 taggagtacc gtcaagtgcg atcatagcac ggtcaatagc ttgttgatca cgtacatcgt
    10201 agccaatgat agcttcagca atgatgttgt ttacgttgtc aacagctttt tgtgtaccaa
    10261 gaccaccgta acgagatttg tcaccgtcgc gaagttcaac tgcttcgtgt tcaccagtag
    10321 aagctcctga tggaaccata ccacgtccga aagcacctga ttcagtgtaa acttctactt
    10381 caagtgttgg gttaccgcgt gagtctagga cttcgcgagc gtaaacatca gtaataattg
    10441 acatttttta ctctccttat gagttaaatt ttttacacct ctataatacc ttaaaacccc
    10501 tcctttttca agaaaaaacg ttatctttgt gcaacttttc cttaacttta taaagtaatc
    10561 gctttctttt gtctgtttta ttctaacttt tatgatatac tgttttcatg acagatttat
    10621 caaaacaatt acttgaaaaa gctcatggtg ggttaaaaat aaatccggat gagcaaagac
    10681 gctatcttgg tacttttgag gaaagagttc ttggatatgt agatattgac acagcaaata
    10741 gccctcagtt agaaaaaggc tttttattta ttttagaaaa ccttcaggaa aaagcagagc
    10801 cactatttgt gaagatttca ccaactatcg aatttgataa gcaagttttc tacttaaaag
    10861 aagcaaaaga aactgatagt caagccacca tagtatctga agagcatatt acttctcctt
    10921 ttggcctggt tattcatagc aatgcaccag ttcaagtaga agaaaaagac cttcgacttg
    10981 cttttccaaa actttgggaa gttaaaaagg aagaaccagc caaaacatcc ttatggaaga
    11041 aatggtttag ctaaatcttg cacatattta ataagtgccc aatattggca gccgtgcgct
    11101 ccagatagag actggcattt ttcaaactat cttctaaagg ttcacttttc tccaaaatag
    11161 aaaagacagc ttggatattt tcaaatggta ggggaggtaa atcttcagca agactaccgc
    11221 aaatagcaat aacaggaact ccaacagggg ttctttttgc aacacctata ggcgctttcc
    11281 cagcaaagct ttgactatca agtcttcctt ctccaacaac aaccaagtca gcatctgaaa
    11341 ctttcttatc aaagttgatt aagtccaagc aggtatcaat tccagacacg atacttgcct
    11401 gagcaaaagc acacaaacca ccagcaaggc ctccacctgc tcctgctcct ttaatttcta
    11461 atgttgcagg tgagaatttt tcataaaaat cttggatcgc ctgatctacg actgcaaaca
    11521 tagtcggatg tcgacctttt tgattgccaa aagtataagt cgcaccttga tgaccacata
    11581 agggacttac gacatctgct aaaatatgaa tttgaacacc ttcaggaatt ttatagcaat
    11641 tttctgttga aacagaagct aagtttaata aggattgacc ggaagcaggc aagacatttc
    11701 catccctatc ataaaattga taacctaaac cagcagcaat ccccagtcct ccatcattac
    11761 tggccgtgcc accaacatcg atataaatat ctttaatccc tttagaaatg agatggagaa
    11821 tcaactctcc aataccacaa gtttggattt gaagtggatt tcgtttctct agcggaattt
    11881 ttccaagacc aaccaagtca gctacttcaa atagtgccag ttcccctttt tgaaaatagc
    11941 gcatggcttc tttttgtcca aaagggtctg tcacttggat ccatttttct tttaggtcaa
    12001 gagaatgtcg gatagcatct acagtacctt ctcccccatc accaacaggg cagaggagac
    12061 attctacatc tgctatcgat tgttggaagc ctctttttat tgcttcagct acctgttgag
    12121 ctgtcaagct ttccttaaac gaatccggtg caattacaat cttcatattt tccctcattc
    12181 taaacagtca atcaaaggaa gaacttctaa aaaatccctc ttgtcaacat gatgtggtat
    12241 ttcttttttg agcatttctt tggaacaaaa ggcgattcct aactccgccg acttcaacat
    12301 taatagatta ttgcccccat caccgattgc caccgttctt tctttagaaa gttttagttt
    12361 ctttctccat ttttccagag tttctttttt gacctgggga cttataattt gtccaactaa
    12421 ttttcctgtt aaaagacctt ctttgacttc aagctggtta gcagtgaaat aggcaatacc
    12481 aagggatttt gctaatctct ccaactattg gtgtaaatcc accagacacc agaccaacta
    12541 ggataccatt cttttggaga atagagatga actctgggac atttagcgat agatgaattg
    12601 agttgaagac gttatcaaag accaaaatag gaagaccttc caacaaggac actctttttc
    12661 ttaaactgct ttcaaagacc aactctcctc gcattgctcg acttgtaatc tgcgaaattt
    12721 ctgcctcatg acctgcctct ctccctaaaa gatcaatcac ttcttctagg attaaggttc
    12781 catctacatc caaaacacac aagcctttta cttgagacat cagttctcct ctctaaacag
    12841 cctaaaaatc gtatgaagtc atcatacgat tttatctatt aattaactaa actatggtac
    12901 aagtcaaggt atgacttgca ggctgtatcc catgagaagt cactctccat agcttgtttt
    12961 tgtaggtttc tccaaatgtc tggatggttt ctatacaagt ccaatgctgt ttggaaagtc
    13021 caatttaacc aataaggaga tagattgtca aagctaaagc cagtaccgct tccttcgatt
    13081 ggattgaaag cgcgaactgt atctcgcaag cctccaactt catggaccaa tggcaaggtt
    13141 ccataacgca tagccatcat ttgagacaag ccacacggtt caaaacgact tggcatgagg
    13201 aagaggtcac aagcagcgta gatttcctga gcaagtttga catcaaaagt gatatttgtt
    13261 gatagcttgt ctgggtaaat ctgagcaaac catgagaaag ctccttcaaa ggctggatcg
    13321 ccagttccca aaagaacaat ctgaacatct tcttgcaaga tatggtgaag actttcgacc
    13381 accacatcaa aacctttttg acgtgtcaaa cgagaaacaa ttcccaccag tggaacgtct
    13441 gctctaacag gcaagccaac tctttcttgc aattttgcct tatttttggc tttcccagac
    13501 aaatcttcct gattgaaatg atagtctaaa agagcatccg tctgaggatt ataaagatca
    13561 gcatcaatcc cattcacgat accagatact ttaccagact ccattttaag aatctgatcc
    13621 aaattacatc caaactgact agtcataatt tcatgagcat agctaggtga aacggttgaa
    13681 acacggttcg catagagaat acctgccttc atccagttca gacagttgtt ccatcgaagg
    13741 gtgccatcag cgtaacgttc aaagccaact ccaaacaaat cacccaacat tccttctgaa
    13801 aattgtcctt ggaattctaa attatgaatg gttaaaactg tttcaatgtc ctcataggct
    13861 tgaatccaac ggtatttttc cttcaacaag aaaggaatca tagctgtatg gtagtcatga
    13921 acatggagaa gatcaggaat aaagtcaatc ctttccatag cctcaatggc agccagttgg
    13981 aaaaaggcaa agcgttctcc gtcatcaaaa tcaccgtaaa catgaccacg gaagaaataa
    14041 tattgattgt caataaagta gaaggttaca ccatttaata ctgttttctt aattccacaa
    14101 tactgtctgc gccaaccaac gctcacctca aaatgaagca catcttcaat ctgatttcca
    14161 aatttagcct ctaccatatc atagtagggt aaaatcactg caacttcgtg cccagctttt
    14221 accagtgatt ttggaagagc gccaatgacg tctcccaaac cacctgtttt tgaaaagggt
    14281 gcaccctctg ctgctacaaa taaaattttc atgaatgaat atcctctgtt actttagcac
    14341 ctttcttaac cacaactgga tgttctgcag ttcctcgaat cacaacacca ggctcaactt
    14401 caaccccttt gtccaagata gcatattcga cctgagcccc ttctccaata acaacacgag
    14461 ggaagagcag gctatcttta accaagctat ccttatggac atggatatta cgtgatagaa
    14521 cagaattagc tacttgacct tcaataatac taccagaggc aaactgagaa gttcttacct
    14581 tagatgtatt agcatagtaa gttggctctt cgtttttgac ctttgtataa atcttttggt
    14641 ttggtgagaa aagagaatag aatttttgtg attcaagcat atcgatattc gcttgataat
    14701 aagatttaac agagtgaata ttggctagat agcccgtgta ctcgtaggcg aaagctccct
    14761 cttttacagc caaatcccgt aaaacatagc gcaatttctc tggatgttct tttttagctt
    14821 cttcttccaa gtgttcaatc aaccaaggtg tatcaacgac aaagatatct gtagacatat
    14881 tgaacgtttc agctgttgac ttgctatcaa agagtttatg agaaagaaca tggtctgttt
    14941 catctacatc caagattgca tttacttctg aaatatcttt cttagctagt tttttataaa
    15001 ctacagtgat aggctctttt gttgtactat gtaggtggaa aacttggttc aaatcaatgt
    15061 taataagaac atcgcagttg agggcaaccg tttggtttga gccagaacgt ttcaaataag
    15121 taagaagctg ttggtagtat tcttttccaa ctgtactact ttctacacgg gtattgtaaa
    15181 ttcctagata gtaatggcta agaaaggttg ataagcccca ctcgcgtcct gaacgaatat
    15241 ggtcaaatac tgagctgata ttatcctgct ggaaaatacc aaagacacta cgaacacctg
    15301 cattagcaag gcttgaaagt gggaagtcaa tcaaacgata tttcccacca aatggcaaac
    15361 ttgctactgg acggtggtcc gtcaatgtcg acatattgtg aaaaccaact gtatttccta
    15421 aaatggcaga atatttatca atcttcatct gttgctaccc ccactacttc attatatcct
    15481 acaacttgta cttcatctgt tccatcaatt tcgacaccgt cagaaataat cgcaccttca
    15541 ccaataatgg cacgtttaat cttagctcct tgaccaatga tagctccact catgataact
    15601 gaatcaagga cttccgctcc ttcgcgaact tgcgcgcctg ttgaaaggat agaatgttta
    15661 acagttccat caacgaaaca tccgtctaca actaatgagt cttccacatg agcatttgcc
    15721 ccgaggaagt ttggtggtga aatcaagttt cttgagtaaa tcttccattg acggttacga
    15781 ctatccaagg cattttctgg agaaatatac tccatgttcg cttcccaaag tgactcaata
    15841 gtaccaacat ctttccaata accactaaat tcgtaagcat aaacactttc acctgactca
    15901 aggtaatttg gaatgacatt tttaccaaag tctgacatgc caaccttgct cttttcagca
    15961 gcgactaaca tattacgaag gcgttgccaa tcaaaaatgt agattcccat agaagctttt
    16021 gtagatttag gttgagctgg tttttcttca aattcaacaa tacgattgtt agcatctgtg
    16081 ttcatgatac caaaacggct tgcttcttta agagggacgt ctaaaactgc tactgtcaag
    16141 ctggcattat tatccttatg agactggagc atatcatcat agtccatttt gtagatgtga
    16201 tccccagaca aaatcaagac atactcagga ttgacactgt cgatatagtc gatattttgg
    16261 taaatagcgt gactagtccc ctcaaaccaa cgatttcctt cacttgcaga ataaggttga
    16321 agaatagaga cacctgaatt aataccgtct agtccccagc ttgaaccatt cccaatatgg
    16381 ttgttgagag caagtggttg atactgtgta acgaccccaa cattatgaat ccctgagttg
    16441 gcacagtttg atagggcaaa gtcaatgata cggtagcgcc caccaaattg cacagctggt
    16501 tttgcgatgc tttgagtgag tttaccgaga cgagttcctt gcccaccagc aagaatcaaa
    16561 gctaacattt catttttcat tttctactcc tttttggttt ttatttgtga cggttttagt
    16621 agatttcaag cgacgtttga ttttccatac acttgctccc atagccggta gggtaaaggt
    16681 taaggtctgc tcataatctt tccatagtcc ttcttgcgtt tgaacagttt gattatgttc
    16741 tttccaaacg cctccccact cttccaactc agtattccat acttcttcgt aaattcctgc
    16801 aacgggtagt ccgattgtaa aatctttccg ctcaacaggt accatgttaa agatacagac
    16861 taacatttct ccctttttac ccttacgaat aaaggaaaga acactctggt ctcgattatc
    16921 cgcatcaatg acttcaatac catcatagct ggtatcgatt tcccacagac agcgatgatc
    16981 tttgtaaaac tggtttagct gagaagtgaa atacttcatc ttagcattca ttgggtcttc
    17041 taggttagac cattccaact gttcttcaga tttccattct aggaattgac cgtattcgct
    17101 acccatgaag agcaatttct taccagggtg acaaatttgg tacgtataga gattgcgcaa
    17161 gcctgcgaat tgattgtaac gatctcccca catcttatgc atcatactct tcttgccatg
    17221 aaccacttca tcgtgcgaga atggcaagag ataattctcc ttgaaaacat acataaagct
    17281 gaaagtcacc aggttaaagt catatttacg ataaatcgga tcttcttcgt agaaacggag
    17341 gatatcattc atccagccca tgttccattt gtagtcaaat cctagaccac caatctcttt
    17401 cattcccgta atcttggttg cagacgaact ttcttctgca atcatcatca catctggata
    17461 ttctaactta ataacttcat tcaagcgttg aaggaaataa taaccttcat agttgagatt
    17521 tccgccatct ttattaggtg tccatggagc atcatcatag tccaaataga gcatgttgct
    17581 aacagcatcc acacgaatac catccaaatg atagatatca atccaatgct taatgcaaga
    17641 aattaagaag gactggactt cattttttcc aaggtcaaaa ttaagggcac cccaaccatg
    17701 gttatgagcc ttattatggt cttggtattc aaaagtcggt gtcccatcat aataggctaa
    17761 ggcatcatca ttgatggtaa agtgacctgg tacccagtcc acaataaccc caatattatg
    17821 ggtatgacac tcctcgacaa aatcttgaaa ctcctctggt ctaccatagg catgctctaa
    17881 agcgaagtaa cccataagct ggtaccccca actcaaacct agtgggtggg acatcaaggg
    17941 cataaactca atatgagtat agttcatctc aactaggtaa ggaatgagtt catctttcaa
    18001 ttgggcaaaa ctataaggac tgccatcagg atttctcttc caagagccag catgaacctc
    18061 gtaaatattg acaggacgtt cagcaaagcc ccaacgtttt cttcgtgcca gccaaagtcc
    18121 atccctccat ttcttatcag gaagctctgt tacgattgcc cctgttcctg gacgagcctc
    18181 atacctgaca gcaaaagggt caatcttcat cagttgatga ccattttgac gtgtgacatg
    18241 atatttgtaa atatgccctt cttgagccat attggtaaag acttcccaga ccccaaagtc
    18301 atttcttacc attggaatct gattttcaat ccaattggta aaatcaccaa ccaagtgaac
    18361 agcctgagca ttaggtgccc aaacacggaa ggtatagcca tactctccat ttagttcttc
    18421 cctatgtgct cctagataat gttggagata aaaattttca cccgtcataa aggtttttaa
    18481 tgcttctcta ttatccatat actccctttc tcctgtaagc gttttctatg tttttattat
    18541 actacctttt tagagaagat tcaagtaaat tactatactt ctttaattat tttgaaaatc
    18601 tacaacaagt tcacttactc gttcaattgt aaatcaatat tttttcaaaa aattgcgaaa
    18661 acacctttct ttttctacta tagtgaaatg aaataaaaca tgcgcaaatc gattaaggaa
    18721 tttaatctaa tttctaacaa tgtcttagaa atcaaagtg
//
LOCUS       DAVBBS010000046        17721 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVBBS010000046 DAVBBS010000000
VERSION     DAVBBS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17721
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.046"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..224)
                     /locus_tag="VQU32_001539"
     CDS             complement(<1..224)
                     /locus_tag="VQU32_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HET2472972.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCT"
     gene            262..1038
                     /gene="recX"
                     /locus_tag="VQU32_001540"
     CDS             262..1038
                     /gene="recX"
                     /locus_tag="VQU32_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HET2472973.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINANQLSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYFDAKIAFDELDSQVDEETTQELIFKELDK
                     QYTKYARKYEGYKLKQRLTQVLARKGYDFSDIASALREYL"
     gene            1127..1660
                     /locus_tag="VQU32_001541"
     CDS             1127..1660
                     /locus_tag="VQU32_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HET2472974.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(2100..2856)
                     /locus_tag="VQU32_001542"
                     /pseudo
     CDS             complement(2100..2856)
                     /locus_tag="VQU32_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567550.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            complement(2994..4616)
                     /gene="groL"
                     /locus_tag="VQU32_001543"
     CDS             complement(2994..4616)
                     /gene="groL"
                     /locus_tag="VQU32_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET2472975.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(4632..4916)
                     /gene="groES"
                     /locus_tag="VQU32_001544"
     CDS             complement(4632..4916)
                     /gene="groES"
                     /locus_tag="VQU32_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET2472976.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(5186..5581)
                     /locus_tag="VQU32_001545"
     CDS             complement(5186..5581)
                     /locus_tag="VQU32_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET2472977.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(5659..6420)
                     /locus_tag="VQU32_001546"
     CDS             complement(5659..6420)
                     /locus_tag="VQU32_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET2472978.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(6453..7079)
                     /locus_tag="VQU32_001547"
     CDS             complement(6453..7079)
                     /locus_tag="VQU32_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HET2472979.1"
                     /translation="MIFTYNKEHVSDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(7095..7412)
                     /locus_tag="VQU32_001548"
     CDS             complement(7095..7412)
                     /locus_tag="VQU32_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET2472980.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(7409..7708)
                     /locus_tag="VQU32_001549"
     CDS             complement(7409..7708)
                     /locus_tag="VQU32_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HET2472981.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(7993..8184)
                     /locus_tag="VQU32_001550"
     CDS             complement(7993..8184)
                     /locus_tag="VQU32_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HET2472982.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(8345..9019)
                     /locus_tag="VQU32_001551"
     CDS             complement(8345..9019)
                     /locus_tag="VQU32_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472983.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLTIRGDVAFGKL
                     EIVYVK"
     gene            complement(9025..9471)
                     /locus_tag="VQU32_001552"
     CDS             complement(9025..9471)
                     /locus_tag="VQU32_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2472984.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(9573..10088)
                     /locus_tag="VQU32_001553"
     CDS             complement(9573..10088)
                     /locus_tag="VQU32_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET2472985.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVCVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLFLHVS"
     gene            complement(10085..10447)
                     /locus_tag="VQU32_001554"
     CDS             complement(10085..10447)
                     /locus_tag="VQU32_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HET2472986.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICSVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(10513..12372)
                     /locus_tag="VQU32_001555"
                     /pseudo
     CDS             complement(10513..12372)
                     /locus_tag="VQU32_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(12389..12859)
                     /locus_tag="VQU32_001556"
     CDS             complement(12389..12859)
                     /locus_tag="VQU32_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET2472987.1"
                     /translation="MDKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVHFLDN
                     REKNQDLVLIKDIEQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQANIKGIGGENKEISQKN"
     gene            complement(12983..13699)
                     /locus_tag="VQU32_001557"
     CDS             complement(12983..13699)
                     /locus_tag="VQU32_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2472988.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(14580..15989)
                     /gene="ply"
                     /locus_tag="VQU32_001558"
     CDS             complement(14580..15989)
                     /gene="ply"
                     /locus_tag="VQU32_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HET2472989.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQHEKITAHSMEQ
                     LKVKFGSDFEKIGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLRQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFESLIKGVAPQTEW
                     KQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFL
                     RDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVLTP
                     KAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRT
                     ISIWGTTLYPQVEDKVEND"
     gene            complement(16000..16332)
                     /locus_tag="VQU32_001559"
     CDS             complement(16000..16332)
                     /locus_tag="VQU32_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472990.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(16403..17011)
                     /locus_tag="VQU32_001560"
     CDS             complement(16403..17011)
                     /locus_tag="VQU32_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2472991.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(17024..17482)
                     /locus_tag="VQU32_001561"
     CDS             complement(17024..17482)
                     /locus_tag="VQU32_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HET2472992.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            17607..>17721
                     /locus_tag="VQU32_001562"
     CDS             17607..>17721
                     /locus_tag="VQU32_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET2472993.1"
                     /translation="MNYEASKQLTDTRFKRLVGVQRTTFEEMLAVLKTAYQK"
BASE COUNT         5233 a         3942 c         3237 g         5309 t
ORIGIN      
        1 atagtacaag atggcacaat tcgaaattta gacttcttgt tgaccttcag taattttgct
       61 tcaacaaagt tgcgtctaat agaagtaatc tgacaataga tatcttcgcc tttgagagct
      121 cctggtacaa agactaatgt tttttggtaa aagccgattc cctcaccgtt aattcccatg
      181 cgcttgattt ttaatggtat tttttgtttc actttcagat tcatacccct atcttatcac
      241 attttgagtt ataatagagc tatgaaaatc acaaaacttg aaaagaaaaa aagactctat
      301 ctgatggagc ttgataatgg cgacaaatgc tatatcaccg aagatacaat tgttcgtttt
      361 atgttatcga gagataaggt gataagcgaa gaggaattga aagagattca agactttgct
      421 caattttctt atggtaagaa tctggccctc taccacttat cctttaaagc acgcactgaa
      481 aaagaagtca gagaatatct gaaaaaatac gatattgata aaaacatcgt ttctcaagtt
      541 attgctaatc ttaaagaaga taagtggatt aatgatggtc agtacgctta tgctatcatc
      601 aatgccaatc aactttcagg agacaaggga ccttatgtac tgactcagaa attagcacaa
      661 aaaggaattt caaaatctac tatagaagaa atcttgaatg attttgattt ttcggaagtt
      721 gctcaacgtg tagccaataa actattgaaa aaatatgagg gaaaacttcc agctcgtgcc
      781 ctgcaagata agattatcca gaacttgact aacaagggct tctcttattt tgatgctaaa
      841 attgcctttg acgaattgga tagtcaagtt gatgaagaaa cgactcagga actcatcttc
      901 aaggaacttg ataagcaata tactaagtat gcccgaaagt atgaaggata caaactgaag
      961 cagcgtttaa ctcaagtttt agcacgaaag ggctacgatt tttcggatat agcaagcgct
     1021 cttagagaat atctttaata tttccaagtg aaattcacag attttaggta attttatggt
     1081 acaatagtaa atgataaact tataaattgt agaaagttgg ttagttatga agcttccaaa
     1141 agaaggcgac tttattacaa ttcaaagtta taagcatgat gggagtctcc accgaacttg
     1201 gcgggacacc atggtactaa aaacaacaga aaacgccatt attggtgtca acgatcatac
     1261 acttgttacc gaaagtgatg gtcgtcgttg ggtcactcga gaaccggcta ttgtttactt
     1321 tcacaagaaa tattggttta atatcattgc catgattcgt gataatggaa cttcttacta
     1381 ttgcaatatg gctagcccct actatctgga tgaagaagca ctgaagtata ttgattacga
     1441 tttggatgtt aaaattttta cagatgggga aaaacgtctc ttggacgttg aagagtacga
     1501 gcgtcacaaa cgcaaaatga attattctga tgacttggac tatattttaa aagaacatgt
     1561 caaaattctt gttgattgga ttaacaatgg acgtggtcct ttctcagaag cctatgtaaa
     1621 catttggtac aagcgctatg tagaactaaa gaatcggtaa agttgtcaaa ggtcaggaac
     1681 aaaatcctga ccttgttttt ttggtagtat aaaataaatt gtgtaaacac aaaaaggaat
     1741 aaatccatta tagcagagtt acaacacatt tctataaaga aatttattcc tacgactcag
     1801 tttggcatgc ttaacttcct aactcaaaaa caaagcatcg attaatttat ccgttcttct
     1861 tttgaaacag atttgaactg ataattgctc gttttaattt aagaagctct tttgagcaaa
     1921 acttcatttt tttaagagtc caacaatttc tgtctatcgc ttttctacta gcaaaaaacc
     1981 tcccttgagg caagttttca tttatatatc taatttcgta acagtatagt aaaatgaaat
     2041 aaggacagtc aaatcgattt ctaacaatgt tttagaagta gaggtgtact attctagttt
     2101 caatctacta taaatttgat atgagaatag tctttctcaa catccttatc caacaagcat
     2161 gctgtggcgt ccttcttaac ttgaaccagg tcaatattct gggcttgggc tgccatagac
     2221 gcagccacaa taacattgac gatagatatc atactcctca cacatctcta ctgaacgttt
     2281 gtagccttga tttttcttga aatcgttggg aagatagtgg gtcgtgtaaa ttttttgcac
     2341 atcgattccg atgctattga tggtttgaga attcttatga ggactgatgg tcaaggctga
     2401 accaaagtag tcaaagccca agtccatagc cacttgcgct gttttatcca gtcggtagtc
     2461 aaaacaaacc ttgcaacggt cgccaccttc gggctcctcc tctagtcccc taactagttt
     2521 tcggtattaa ttgggttcgt agggagcttc taggtactga accgtatttc ctgtccgatc
     2581 attaaaatca ctaacaaatt tcttgatgac atagacccgc ttatggtatt ctactttggg
     2641 atggatatta gaattggcaa aatagatggt cacatctgca tacttggtca aatattctag
     2701 tgtataggta ctacaagggg cacagcaaac atgcacgaga atggttggcc gctgctcatt
     2761 tttctcccat gcttgtacca ttttctgcat gacacggtca taattaatct tctgattggg
     2821 gggcatcttg cacagaattt cttctacatc gatcatgttc ttctcctttt tctagtctta
     2881 ttttatcata taagttagaa gagctcaaat caatttagaa gtgaatatta taaaaagaag
     2941 ggttagtcat tccctccttt tgtgtttttt ataagttgtt ttctatagaa agcttacatc
     3001 atcccgccca tcatacttgg atccattgct ggagctgggg ctactggttc tggtttattg
     3061 gctacgactg cttctgttgt caaaatcaag ctggctacag atgctgcatt ttgtagggct
     3121 gaacgactca ctttaactgg atcaatgata ccttgatcaa tcatgttaac ccactcgcca
     3181 gttgctgcgt tgaatcctat accaagctca gcatttttca aacgatcgat aacgatagat
     3241 ccttcaaatc ctgcattgtg agcaatttga cgaacgggtt cttccaaagc acggagaaca
     3301 atattacgtc ctgttgcttc atctcctgtc aattccaagg tagcaacagc tggaatcaca
     3361 ttggcaagag ctgttccacc acctgcaaca ataccttctt caacagctgc acgagtagcg
     3421 ttgagggcat cttcaatgcg gagtttcatt tctttcaact cagtttcagt tgcggctccg
     3481 accttaataa ccgctacacc acctgacaat ttggccaagc gttcttgcaa tttttcacgg
     3541 tcaaattcag aagttgtagt ttcgatttga gacttgataa ccgcaacacg gtgagaaatc
     3601 gcttcaggat ttcctgcacc ttctacaata accgtgctat ctttgtccac ggtcactctc
     3661 gctgcttgac caagagcttc aattgtcgca tctttcaact caagaccaag gtcttctgtg
     3721 ataactgttc cgcctgttaa gatggcgata tcttcaagca tggctttgcg acggtcacca
     3781 aaaccaggtg ccttgactgc tactacgttg aaggttccac gaatcttgtt caaaacaaga
     3841 gttggaagag cctcgccatc cacatcatcc gcaataatca agagtggacg attgctttgg
     3901 agaatgcttt ccaaaagtgg caagatttct tggatattgg aaattttctt gtctgtaatc
     3961 aaaatgtacg gattttcaag gtcagccacc attttttcgc tatcagtcac catgtactgt
     4021 gaaaggtaac cacggtcaaa ctgcattcct tctacgactt caagctctgt ttccatacca
     4081 cgtgactctt cgatggtgat gacaccgtct ttgccaactt tttccattgc ttcagagatg
     4141 tactcaccaa ctttttcaga acgagaagat acggctgcaa cttgagcgat agcttcttta
     4201 ttggcaacag ggatggcgtt gtttttcaaa gcttcaactg ctgcggcaac tgctgtttca
     4261 atcccacgac gaataccgat tggatttgca cctgctgtga cgtttttgat tccttcacgg
     4321 acgattgctt gggtcaaaac agttgcagtt gtagttccat cacctgcgat atcattggtt
     4381 tttgaagcca cttctgatac caatttggca cccatatttt caaaatggtc ttctaattca
     4441 atttctttgg caatagtcac accgtcattg gtaatcaatg gtgagccaaa tgatttttca
     4501 agaacgacat tgcgaccttt tggtcccaag gttactttaa cagtgtctgc aaggatatcg
     4561 acaccacgga ccatagctga acgggcatct gatgaaaatt taatttcttt tgacatactt
     4621 actttctcct tctattcttc aatgattgcc aagatgttag cttcgcctac gatgatgtac
     4681 ttttcatcgc catctttgac atcaagacct gcgtgggctt caactaagac acggtctcca
     4741 gttttaacac ttggagcaac caagtcaccg ttcaaggtac gaacaccttg tccagtagcc
     4801 acaacttggg ctgttttggt tttttcttgg gctgaacctg cgagaacaaa gcccccaacg
     4861 gtttgttctt tttcttctat ttttaagacc acacggtccc ctaatggttt caacatctga
     4921 tttcctccat aatgagatag atattattag cgctctttat acctgagtgc taattcatag
     4981 ttctattgta tcacttagtc agaaataatc aagaaaaaag tctgactttc tcaagataaa
     5041 aagcctgaga ccaactcaga ctttttaatg tttatactca atgaaaatca aagagcaaac
     5101 taggaagcta gccgcaagct gctcaaagca ttgctttgat gttgtggata aagctaacgt
     5161 ggtttgaaga gattttcgaa gagtattaaa atggcaattc ttcctcttcc aagactaaat
     5221 ctgccaaatc ttggcctgca ttattttcac gcatggcacg ttgagcacga ctttccaaga
     5281 gttggaatcc tgttacaagt acttcggtca cgtagttcat ttggccattt ttctcaaagc
     5341 gacgggtacg caattctcca tcaacggaaa tgagactacc tttggttgcg tagcttgcca
     5401 aagtttctgc tagtctgccc cataggacca tattgacaaa atcagcttca cgttcaccgt
     5461 tttggtcttt gtaacgacgg ttcacagcga tagttgctcg cgctaccgac ttgtcattgt
     5521 tggttttgtg caattctggt gtagacgtta aacgcccaat catgataact ttattataca
     5581 tattttcttc ctcctactta tctattcgta ggaaatcaaa aaaagttaca gaaatttgta
     5641 acttttcgag gaaatttctt attttttatg aatcatgaaa cctgtcgcct gttgattggc
     5701 cataatggtc atatctgtaa tctgaacacg gcgaggctga ctggtcacat agactactgt
     5761 gtctgcaata tcctgagctt gcaaggcttc tattccttgg taaacggacg cagctcgctc
     5821 tttatcacca tgaaaacgaa cagttgagaa atctgtttcg acaatcccag gctgaatggt
     5881 tgtcaccttg atatccgttg cgatggtatc aattcgcagt ccatccgaaa aggtcttaac
     5941 cgcagccttg gtagctgagt aaacagcggc accagcatag gcgtagattc ctgcggttga
     6001 ccccatattg ataatatgac cttgattgac ttttatcatt gctggcaaga aacagcgagt
     6061 aaccgccatc agacctttaa cattggtatc caacatggtc aacatatcca actcctcata
     6121 gtcttgatag ggagccaagt caagagccag tccggcgttg ttgaccaaga tatcaatcct
     6181 ccctatcgtt tctagaatat cagagcaaac agtcttcacc atgaccatat ccgtcacatc
     6241 tagtggaaaa gtccagacgg tttgatttgg aaagcttact gcaaactccg acttgaggat
     6301 ttctaatctg tctatccgtc gtcctgttag aacgacatcc tcaccctgct ccagataagc
     6361 acgcgcaatc gcttcaccga ttcctgaggt tgctcccgta atcactacat tttttgccat
     6421 cttatttcct tctatcagat gctgacaagt tcctaggcag tccagtgttt cgctgggtcg
     6481 aacggtgttc cgacaacttg gtcttctgat aattcaagca ccccacgttt ttgcggagca
     6541 tttggcaaat gcaattcacg aggactacac atcatcccaa aactcttttc accacgaagt
     6601 tcgcctggga aaatgaaatt cccttttggc atcatggcac caggaagagc cacaatggtt
     6661 ttcaacccaa cacgcgcatt aggtgctcct gcaacgattt gcactatctt gtcacttgcg
     6721 actgcaactt ggcagatgtt gaggtggtca ctgtctggat gagctaccat ctcgacaatt
     6781 tcaccaacaa caaacttagg ttccttatca ttaacaattt cttctgtaaa accttccgcc
     6841 tgtaattctt ggttcaaacg agcgacttgc tcatctgata aaaagacttg accgcgctct
     6901 gcaatttcaa acaagctcga aacctcaaaa atgttccaag ccactgtttc cccattttct
     6961 ttgaggaaaa cacgggctac cttgcctttg cgttccacat tcagtttggc atctccgcta
     7021 tttttcacga tgaccataag gacatcactg acatgttctt tgttatacgt aaaaatcatt
     7081 gtttcctttt tctcctattt caatcctgct aaaaaatcgt taatttgctc cttactttta
     7141 cggtcgcgat tgacaaaacg accgatttcc ttgtcctttt ctagaacaac aaggctagga
     7201 attccgtaaa catcccagag tttggccaaa tccatatact gatctcggtc cattcgaata
     7261 aaggtgaact ctggattggt ctcttcaatc tctggtaagg caggataaat ataacgacaa
     7321 tcgccacacc agtctgccac aaaaaggaag accttcttgc ccgctttttc cactaaagat
     7381 gctaattctt ctaaacttgc tggctgtatc ataagacttc ctcctcatag actaggtctt
     7441 cattttcata gacaaaggta taatgacggc catcctcaaa aatgacgcca ccaaccaagc
     7501 tctccagact gctttcgtaa acttgaacat aaagggtcgc aatttccccc atgtcggaaa
     7561 aatggtctcg cacaatctct gtcaactctt cctgagtctt catgagctta cggtcatctg
     7621 caactttttt cgtagcaaga gcaaggcttc cgatacctag cagagccaag cctgccatcc
     7681 acattttttt agctttcata ccattcattt taacacaaaa aaggcttcag gacaaatgag
     7741 gaagcagcag aaaagcaagt aaaaagcctc ttcctttaag gaaaaagact tcttatacta
     7801 agagcaaact aggaagctag ccgcaggctg ctcaaaacac ggttttgagg ttgtagataa
     7861 ggcgacgttg acgtggtttg aagagatttt caaagagtat tattcttatt gccaggcacc
     7921 taagttgcca acgtagtaac tatcaggtgt gtaggtattg cgaagcatct tacctgatga
     7981 agccagataa tactacttgc cattgtcttt gacccaatca ttcgcaatca tggaaccaga
     8041 agaacttaca taataccatt ctcccttgtc ataaacccaa gtactgactt tcatggttcc
     8101 tgagcaatta aaggcaaaaa aactgtccaa tatcattcgt tttttaaaag catttgacac
     8161 tacatcatct acgctgtagt acatagatac atcaaacgta aaagccaaaa tatcgaaaaa
     8221 tatacaactt ttaaaggcag catttcattt tttaaagtct aacaaccata gaactgaaat
     8281 cagagcaagg catcaatttc ctactcttag tacgtctatt ttgatggttg aagtgaagat
     8341 ttttttattt aacgtagaca atttccaact tcccaaaagc cacatctcca cggatagtca
     8401 aggttttgct ggttggggct acaggagcgt cagccttagc tgcaccaaag gaggtttcta
     8461 ctttcaaatc tacacgccag tgttgtggaa catagacggt tgcattcccg aaggccactt
     8521 caatatttaa agttgcaaaa tcacctagca tctctgcatt atcatagtag attttagcat
     8581 ccccaaaagc gacttccact tggtcatcta cgagatcttg atcttgctta tagaaggtcc
     8641 cgctaccaaa agcgacttcc ttatccgtga cgactgtttt tttcccattg taccaccatt
     8701 ttttttcatt ccagaactta cttgaatgcg tcagataacc aacaccaagt accaccaaga
     8761 tgctagccca aataagagaa tgattggtaa ctggtaacaa gtcataagcg taatttgcaa
     8821 tgatgagcgc cagtaaacct gtaaaaactg ccgaagtgag atgacgtcta aggatggact
     8881 caatggactt ataagcaaaa aaaacaatac ctagtaaagg ccatatttta ccatccaaag
     8941 aaggaattcc aaaattccct tgcagcaaga tccaagctgc taaaaccaac aaaacaatac
     9001 caaatgcttt ctttttcatg ttttttacct catgtttctt agattttctt ttaggaggga
     9061 ttggtaatgc cgtgagacat gaacctcctt gtgcgtctga taaaaggaaa tggtgcccgt
     9121 tcctgaaaag gacttgtcca ctgagtaaat ctgacggatg tttgcgatcg ttgacttgga
     9181 aactcgatta aaatagcgag gcaagataga ctccaactca tagagcttga ggcgaacctc
     9241 ataagcttcc ttctggttat gagcgtagat cttgctccct tctgtttcaa agaagagaat
     9301 ttctgacagg tctagataat attcacctgt ccccttgtaa aaagtcaacc tcggagcctt
     9361 tgactcttgc aagagccgtt gcaagtctgc aatttcatct gtcaaagtgg gggtcttgat
     9421 gacaatttca gtctcctcta aattgccgtc aatctcaatt cgtaacttca tcacatctcc
     9481 tttctgactc tactatatca aagctaagtt aggaatacaa gggcaaaaaa gcaagtggtc
     9541 aatttgaagc agtaagtggt tgtaggacta gcttaactta catgtaagaa taggaagaaa
     9601 aggaatcccc acaccaaacc acaagcatag ccaaccaaga catccttggg ataatgaaca
     9661 ccccctaaaa ccctcaccaa ccccagcaaa gctgaaaaga tcaagcatac taagccaata
     9721 ggtagactag catgcagaca ggccatggag atgatagttg ccgaaaagac atggcgactg
     9781 ggcatcgacc gtcccgaact atccttatca agaaggggac agatatccca agtttcataa
     9841 ggtctcgggt gattgatttt cttacgaaac agggacaagg ccacaaaacc tgatgcagga
     9901 ataagcacac aaaccccaac ttgctgacca agtcctagtt gcaggtaagt ggtgactagc
     9961 aagatcagat agaccagggg catggcgact gtcatcaaac gattgaaact gcgtaatagg
    10021 aataaaagaa ttggacgact agttagtctg gagctgatat ttcgatacca ttcttgataa
    10081 tttttcattt atgcttctca tacttttcta gaacttgttg agatgttttc cccttggcaa
    10141 gccaatccac tagcttgtcc aagcgacgta tttcctgcat cagaggatca tcaatttcct
    10201 caatacggac actgcaaatc ttccctgtaa ttgcaattcg ttcagggaca taatatggtg
    10261 cttgtctaaa aaaatctccg taagtaacat ccctatccag acaagcaaga acatcactca
    10321 cttcataccc tgtcaaccaa ctggtcactt ggtgaactga ctctgccttt ccaccctttc
    10381 gctcaacttt tgcaatcaag gctaagtaga cagcagcaaa tttcatattg tataatttct
    10441 gactcatact tctctctttc tatgtgtact agtataaata agaaaaagaa ggccgtcaag
    10501 ccttcttttg atttattctt ctgcttcatc ttctgtaaat tgactattgt acaagtcagc
    10561 gtagaagcca ccttgcgcca tcagttcctc atagttgcct tgctcgatga tatttccatc
    10621 tttcatgacc aagatcaagt ctgcatttcg gatggttgac aagcggtggg caatgacaaa
    10681 ggatgtgcgt ccttccatca aacggtccat ggctttttgg atcaattcct ctgtccgtgt
    10741 gtcaacagaa gaagtcgcct catccaaaat caaaagcggt gcatccttaa gaagggcacg
    10801 agcaatagtc aatagttgtt tttgtcctac agacaaggtc acggtgtcat ccaagatggt
    10861 atcatagcca tctggcaagg tcataataaa gtggtgaatt cccacagcct tactagcttc
    10921 catcattcgt tcatcactaa tccctatttg attatagatg agattgtctc gaatagttcc
    10981 ttcaaagagc caggtatcct gcaagaccat tgaaaaggca tcatgcactt ctgaacgcgt
    11041 catagccttg gtatccacac catcaatgtg aatacttccc ttatcaatct catagaattt
    11101 catcaaaaga ttgacaatgg ttgtcttacc agtcccagtc ggcccaacaa tggcaacctt
    11161 ttgaccagca tgaactgtcg cagagaagtc atagtcttga acattgacac cgtccaccag
    11221 aatttctcct gctgacacgt cgtagaaacg tggaatcaga ttgaccagag ttgatttacc
    11281 agaacctgtt gacccaataa aggccactgt ttgaccagtt tctgctttaa agctaacatg
    11341 ttcaataact gcctccgaat ttgccgcata gcggaaggtc acatccttaa actcgacctg
    11401 acctttgaag ttttcatcag tcagctgcac ttgaacaggg ttttggatag aagaatgcaa
    11461 atctaaaact tgattaatcc gcttagcaga gaccatagtt tggggaagaa cgatgaagag
    11521 tgctcccatg agaaggaagt ccatgacaac ctacatggca taagacatga aaacaatcat
    11581 gtcactaaag agaggcagac gcgctatcgg agcagcgtcg ttaatcacat aggccccaat
    11641 ccagtaaatc gccacactca aaccacttga aatccccatc atgataggat tcaaaatagc
    11701 cataagacgg ttgacaaaca aatttaaacg ggtcaattca tcatttactg ctgcaaattt
    11761 ttcattttga taatcctctg cattgtaggc acgaacgaca cgaatacctg ttaaactctc
    11821 acgagtgata ctgttcagtt tatctgtcag cccctgaatc aaggactgtt ttggaaaggc
    11881 tagcgtcatc aaaacggtcg tcatcaggac gttgacaatc actgccacaa gtacggccca
    11941 gagccagtat tctgaatgac ctaaaatctt cccaatagcc cagatagcca taattggacc
    12001 acacgttacc acttgcaagt ccatagtaat caacatttga acttgagtaa tgtcattggt
    12061 agtacgcatc aagaggctag gaattgaaaa tttcttaatc tctgtctgcg agtaatccaa
    12121 aactcggtta aaaatatcac ttctcagcct actagtataa gaagccgcca ctcgggatgc
    12181 aaaaaatcca actgcaacta cggacaagaa ggcaagaaag ggcattccca tcatcatgct
    12241 tgccggctgc cacaactcat ctaaattagt tccttgacta cctagcaaat ccgtaatttt
    12301 cgagatatag gtcggcactt ccaactctag atagaccgaa aagcaagtaa agagaatggc
    12361 tagtaaaatc atcccccatt cttttctact aattcttttg actaatttct ttattctctc
    12421 ctcctattcc cttgatattt gcctgtagtt gaccgagaac tttctcaaaa atcagtaatt
    12481 catcttcatc aatgtcttcc atcaactgct tgtctatgcg ttcaaaaaaa gccttaacct
    12541 gttgcatctg agaacgtgct ttgtccgtca gacgaacaaa cttagcccgc ttatcaacag
    12601 gactcgcctc caattccacc aaaccatttt gcactatacg cttaaccaga ttactagcaa
    12661 cagacttggt aatattgagt tcctgctcga tatctttaat caagaccaag tcttggtttt
    12721 tctcgcgatt atccaaaaaa tgcacaacct gaccttgcgg cccacccata aattcaatgc
    12781 cgcaacgttt ggcttccttt tgcaccatca ggtgaatttg atgaccaaaa cgcttaaaga
    12841 ctaacatcgg tttatccata atctccccct tctaaataaa aatagttctc tggagaataa
    12901 ttaaatttct atgagaacta ttttcttgat taaaaaaatc ccaagtgatt ttctcactta
    12961 ggatcatgtt ctataggtta aattaaaacc catctacgtt cgtataaatc ttttggacgt
    13021 cttcgtcgtc ttcaagaacg ctgtaaagtt tttcaaaggt ttcaaggtct tcgcctgaca
    13081 attccacttc tgactgagga atcatttcca attcagtcac ttggaattct tcaataccag
    13141 actcacggag ggcaacgata gccttgtgaa ggtcagttgg cgctgtgtaa actgtgattg
    13201 taccttcttg tgcttctacg tcatccacat ccacatccgc ttcgagcaat tgctcaaaga
    13261 ctgcgtccgc atcttcacct ccaaatacaa taacaccttt gttgtcaaag aggtaagaaa
    13321 cagaacctga agcgcccatg tttccgccgt ttttaccaaa ggctgcacgg acattggctg
    13381 ctgtacggtt gacgttagaa gtcaaagtat ccacaattag catagagcca tttggcccaa
    13441 aaccttcgta acgtccttct gtaaaggttt cgtctgtgtt tcctttggct ttatcaatcg
    13501 ctttatcgat aatgtgtttt ggcacttggg cttgtttagc acggtcgata acgaatttca
    13561 aagctgagtt tgattctgga tctggatcac cttttttagc tgctacatag atttctacac
    13621 caaattttgc atatacttta gagttggctc catctttagc cgttttcttg gctacgatat
    13681 tggcccattt acgtcccatt aggaatctcc ttttttcaca ttttaatctt tcttattata
    13741 acacaagttt ttttgatttt cactagagga aatggatttt attagcaaat caagctagga
    13801 tagcacttta cctgctaaga tggtcttgcc tttctatctt tatcaacaga cactcatcca
    13861 cattcaaaaa acaaactaga ccattatctg caaatagaaa gtttcagcca agtttgacaa
    13921 agtcagctca aattactgtt tgaagtttgt agatataagc gacaaaaaca atcatactgc
    13981 accttttgtt gacagtctac tccagacata tcatagttca agtaaatact ttgaaatcca
    14041 acagttctta taggcgctat tgtattctaa gaaatcaata gaagagtttc taagcaaacc
    14101 tctaatactc aataaaaatc aaagagcaaa ctagaaagct agccgcaggt tgctcaaaac
    14161 actgttttga ggttgcggat agggctgaca tggtttgaag agattttcga agagtataat
    14221 ttacgtgttc ctaagatgga gaagttagac tagtacactg gcacttctaa accattgcta
    14281 gcaattgatt tgttcatatt taatttcatt ttttccataa atgggtatta gatataaaca
    14341 gcaaaatatt tccgatacgt gtcattcttg aatttccaat catctaaaac aagtaaagga
    14401 taatcaatcc cctgtatatc aaggaattgg ctaccctttt tactttttta cacatcctgt
    14461 ttgatagact cattttaaca tcacgagcat actccaatgg aaatcgctag gcaagagata
    14521 aactttcaga tattcgcaga gagctcatcg cctctttttg tcgcaaacat tcttctctcc
    14581 taatcatttt ctaccttatc ctctacctga ggatagagag ttgttcccca aatagaaatc
    14641 gtccgcttac gcactagtgg caaatcggtt ttttcataaa ccgtacgcca ccattcccag
    14701 gcaagcccgg tacactctct aattttgaca gagagattgc gaacattccc ttttaaagga
    14761 atactagtgg taaagtgagc cgtcaaatcc tgcccatttc tgtcccaagc cttaggagtc
    14821 aagacttcct tgccttgatg atcataggat aattcatccc aagtaatata atattgggca
    14881 acataggcac cactatgatc cagcagtaaa tctccgtttc tgtaagctgt aaccttagtc
    14941 tcaacatagt ctgtactgtt ttgaaaggtc gcaactacat tgtcacgtaa aaaagaagtt
    15001 gtataggaaa tcggcaagcc tggatgatcg gctgtaaagc gactgccttc ttgaatcaag
    15061 tcctctacca tatccacctt gcctgttaca actcgggcac ccgaacttgg gtcgccccct
    15121 aaaataaccg ccttcacttc tgtattgtcc aaaatctgct tccactctgt ctgaggagct
    15181 actcctttta tcaaagattc aaaagcagcc tctacttcat cactcttact cgtggtttcc
    15241 aacttgagat agacttggcg cccataagca acactcgaaa tatagaccaa aggacgctct
    15301 gcagaaattc ctctctgcct taaatcctct accgttacag tatcttgaaa cacatctcct
    15361 ggatttttaa cagcatctac gctgactgta taataaatct gcttaaaatt aacaatctga
    15421 atctgctttt cgcctgaatg gacagagtta aaatcaatat caagagaatt ccctatcttt
    15481 tcaaagtcag aaccaaactt gaccttgagt tgttccatgc tgtgagccgt gattttttca
    15541 tgctgcattc tagctgggac attattgacc tgaccataat cttgatgcca cttagccaac
    15601 aaatcgttta ccgctccgcg aacacttgaa ttgctggggt cttccacttg gagaaagcta
    15661 tcgctacttg ccaaaccagg caaatcaata ctataagtca tcggagcacg atcgaccgca
    15721 agaagagtgg gattattctc taacaaggtc tcatccacta cgagaagtgc tccaggatag
    15781 aggcgactgt cgttggtagc tgttacagaa atatcacttg tatttgtcga caagctccgc
    15841 ttctttcttt cgataacaac aaactcatcg ggtagctgat taccctcttt gatgaaacga
    15901 ttttcaatac tttctccctg atgggtcaag agtttctttt tatcgtaatt catagctagt
    15961 ataaagtcat ttactgcttt atttgccatc ttctacctcc taataagttc ctggattgag
    16021 ttgcataaac tcagacttgt tcagcgaaat cagccgtggt tggactaagt aatccaaaat
    16081 ttcctcgtac aattcttctg agacattgcg tcgccgtctg gctaaataag aagtcggaat
    16141 gaccgtatta tccaacataa ataccttatc taagtcaatc aaggttggtc ttgtaaaagg
    16201 attacgagct agatccggct cttctatcat aaagttcttg accaaacgtc tggtcaagag
    16261 agctggtttg aaggtctgat ttttaaccaa ctctttgttt ttagtcatgc tgttgtcaat
    16321 acagatatac atatgattct tcacagccaa atcgctacta atagtcggaa aaggcaaata
    16381 aagagctaca acatctcctc tcttaatcaa gcaagagcac ccccttttct cctaatgtaa
    16441 catagacagg attgaccaag tcttctgatt gactcagaat ttccaaagtt tgagtttggc
    16501 gcgctgtcaa tttagtagca tcttgtctct tcaatacaaa atgcttgtcg ccaataacct
    16561 tgacaatata atccttctcc aaagctgact ggtaaatcca catcagatgt tgtctgtcct
    16621 gagaactcaa gagagaagga ttttcaagcc tcccgatagt ctgataaaaa tcaaaaacag
    16681 gagctaactc ctgccaatct gattggctag ttgtcaaggc tagaaaaagg gctttgcgag
    16741 ctgatacttc ttggttagcc ttgagagtta ctttcccctc caagtttttt agaaatcggg
    16801 aaactccaga aagcaaattt ttctctaact gcgagaaata aaaacctttc gttcccagac
    16861 ataagtcttt catgtcgctt tctctagcaa ataagagctc aaacatttga tagtaaaaga
    16921 aaaatatctg gcactgggtc gcgctcatct tttccttatc ggcttctttt tttaaccaga
    16981 gcaagggcga caggtagctg gattgagaca tttcctctac ctcctactct tttttaactg
    17041 gagcatctgc actagctgcc acttcttttg actggatact ttcccactgg ttaatctcct
    17101 ctgagataag accttcgcat gtcttgacaa atagggcaaa agccttggtc tttcctgcat
    17161 atttctccgt ttggcattga tagaggaatt tttctttctc caggagttgc gcagtttttt
    17221 ggtaagaaat ccaattttcc tttgcattat acaaattgat aatcccctca cacagcaagc
    17281 cgagactgga taaggcaacc gaaatcaaac ggtagcgatc acctggcata ggaatagcac
    17341 aaaagacagc tatgaggaaa cctgccacga tttctgttat ttttaatacc ttatagcgcc
    17401 tacgatgttg aacgcttttc tttaaaaaat gagctatctg tacgtctaat cgctctgtca
    17461 ggtacatttc ttctggcgtc atattcgtaa ctcctttcat ttactttgat aaccatagta
    17521 taacatattt gaatatcaaa gtatagtaaa aactggacta taacttatta gactccctgc
    17581 gaaacaaaat atggtatagt agttctatga attatgaagc aagtaaacaa ttaactgata
    17641 cacgatttaa gcgtcttgtt ggtgttcagc gtaccacttt tgaagagatg ttagctgtat
    17701 taaaaacagc ttatcaaaaa a
//
LOCUS       DAVBBS010000047        17572 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVBBS010000047 DAVBBS010000000
VERSION     DAVBBS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17572
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.047"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(179..1114)
                     /locus_tag="VQU32_001563"
     CDS             complement(179..1114)
                     /locus_tag="VQU32_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HET2472994.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(1111..1971)
                     /locus_tag="VQU32_001564"
     CDS             complement(1111..1971)
                     /locus_tag="VQU32_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET2472995.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFYYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            2079..4241
                     /locus_tag="VQU32_001565"
     CDS             2079..4241
                     /locus_tag="VQU32_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HET2472996.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATITSYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIATLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIILSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYFYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            4333..5592
                     /locus_tag="VQU32_001566"
     CDS             4333..5592
                     /locus_tag="VQU32_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2472997.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADDTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRSE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            5606..6472
                     /locus_tag="VQU32_001567"
     CDS             5606..6472
                     /locus_tag="VQU32_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2472998.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            6486..7319
                     /locus_tag="VQU32_001568"
     CDS             6486..7319
                     /locus_tag="VQU32_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2472999.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKSTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIIAGISNGAV
                     K"
     gene            7320..7400
                     /locus_tag="VQU32_001569"
                     /pseudo
     CDS             7320..7400
                     /locus_tag="VQU32_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            7538..8980
                     /gene="gtfA"
                     /locus_tag="VQU32_001570"
     CDS             7538..8980
                     /gene="gtfA"
                     /locus_tag="VQU32_001570"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HET2473000.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTEILPEIHEHYSIQFKIADHDYYVYDFTLPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAREVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVSF"
     gene            9118..10311
                     /locus_tag="VQU32_001571"
     CDS             9118..10311
                     /locus_tag="VQU32_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET2473001.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            10998..12977
                     /locus_tag="VQU32_001572"
     CDS             10998..12977
                     /locus_tag="VQU32_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2473002.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            13044..14540
                     /locus_tag="VQU32_001573"
     CDS             13044..14540
                     /locus_tag="VQU32_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2473003.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            14540..15466
                     /locus_tag="VQU32_001574"
     CDS             14540..15466
                     /locus_tag="VQU32_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2473004.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSIRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            15475..16542
                     /locus_tag="VQU32_001575"
     CDS             15475..16542
                     /locus_tag="VQU32_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2473005.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            16553..17479
                     /locus_tag="VQU32_001576"
     CDS             16553..17479
                     /locus_tag="VQU32_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2473006.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
BASE COUNT         5175 a         3141 c         3674 g         5582 t
ORIGIN      
        1 aggctccata atatccatag ggaatttacc cactacaaat attatagagc ccgttttaat
       61 gagagtttgt aactctcttt tttgttttta gagacaataa aacttcagat ccgtaaaact
      121 taattgaatt actaatctga agttattttt atatcgttta tattataatt gtgttatttt
      181 acttccaact attgagagaa atttcgccac tatttagcca gatttctttt ccgttatcac
      241 attgaactaa aagttttcca ttttctgaga tgtctttagc aagtcccttg tagtcttttt
      301 gctctagtgt gaaagtgact tcttttccta gaatgaatga ctgttttttg tataggtata
      361 atagctcttc tgctggtgtt tcgaagaaag cacgccagat ttctatgatc aattcattcc
      421 ttgttatagg agctgtagct ttaaataagc tggcagcttt ttcttttaat tcctgaggga
      481 agtctttaat agtgaaattg atacctactc caataatgat atctgtgact aagccagttt
      541 ctacagaggt cattgcttca gtaaggattc ctccaatttt atgattgttt agatagatat
      601 cattgaccca ttttatgtcg acatctatta aagttaggtt cttaatggct ttgtagacag
      661 ctccagctac aagtagtgtg taggatggta atttgtcata ggggagattt ggtttaagat
      721 ggagtgtcat ataaatacca ccttgtggtg agtagaagga acgttgaaaa cggcctcggc
      781 ctgctgtttg ataggaagct agatagaggg tatttgcttc atggcctaaa tcaattgctt
      841 cttttgcatc tagttgtgtt gattttgttt cgggtttaaa gctgacttta attggaagat
      901 tttcttctag aatctctgga agaataaggt caccattcat cagtttatat cctctatttt
      961 tgatactatc aatttcaatg ccttcttgtt ctagtcgctt gatggctttc caaattgctg
     1021 ttcggcttag ggatagtttt tctgcgattt tttctccgct gatatagtcg gtttctttag
     1081 ataggatttg gtagacagct tggtaggatt tcataatgtt gcctttctca ctagttggta
     1141 ttgagagtat tcttttcttg tatgacttgg agtctgatta aagtattgtt tataagcttt
     1201 cgaaaaatgg agtggatctg aaaaacctac cgagtatgca attaccttga tggactcttg
     1261 ggtattttcg agaagttgtc tagctcggtg cattcgaacg tagagtaggt attctttggg
     1321 tgataaggta ttaaattctt tgaatacgct tgataagtag cttctgtgaa cggatagttc
     1381 ttttgctaaa tcttgaattg taagtgattg aggatagtgg ctatcaatta atcgtttgca
     1441 ttcaagatag agttggtggg ttgatgaaat attctttttt ttctgattgg gagcaatagt
     1501 tcccagatga aacatcagtt catgaagttg tcccatgata tggagttgag ctaattcact
     1561 tgattttgta atctgagcga agcggacaat gtctgagatg agttttgcag tagtctgggt
     1621 atgacaagtt tcagattgga tgagatagga ttgatcagaa atttgagaaa gagcaaaata
     1681 atcaggggat ttccctccag tgattcctaa ccagtagtag gcccaaggtt ctttactatc
     1741 tgcttgataa aaggttagtt cctctggttt taatagaaag aaatctcctt cttttaaatc
     1801 aacaatttta cccttgtaat aaaattttcc ttgtccttta gtaatgtaat gtagaacgta
     1861 tgtatcacga atggctggac caaaagagta attaggtgtg cattcctcat atccataaaa
     1921 gcttagggca aggtcgattg ttccagtctg gtattctgaa aaaactaaca tgtgctacct
     1981 cctacctaac attttaccat attcttgaga catttttcta ttttggaaag cgatttcagg
     2041 tgataaaata taatcaataa agtgatgagg tgaagtaaat gggagttagg atagagaata
     2101 atctatttta tgttgagagt aaaaatctaa gtttgattat tgaaaatcga aatggctact
     2161 tacttttgaa acatttagga aagactatta agaactataa aggttccaat agtgtttatg
     2221 aacgagacca tgccttttca ggaaatccaa cggctactaa tcgaaccttt agtttagata
     2281 ctcagcgaca gatttttgga caacatggct taggagattt taggaaacca actatacagg
     2341 ttcagcatag tgtaactgaa gtaacagact ttcgatttgt agaagcaaag attttaaaag
     2401 gtcagaatgg tccacagggc ttaccttctc cacatagcat ggacgataca gagactcttg
     2461 tcttaatgtt agaagattct aaggctcaac ttagtctgac tttgtattat actactttta
     2521 ataatgatgc gactattact agctacagta aattagataa taatagtaat caggaagttg
     2581 tcatccataa agatttttct tttatggctg attttccagc tgcagattac gaaatagcaa
     2641 ctctgcaggg tgcttatgct cgtgaaaaga ctgttagacg tcaacaggta gaacaaggaa
     2701 tcttttcgat tagttcaaac cgaggtgctt ctggtcatgc tcaaacacca gctcttctac
     2761 tatgcgaaca aggagtcaca gaggatgctg ggaatgtgtt tgctattcaa ctaatgtata
     2821 gtggcaactt tgaagctttt gttcaaaaaa atcaattgaa tgaagttcgg gtggctattg
     2881 gcattaatcc agaaaacttt tcttggaagt tagcccctga ggaatacttt gaaacaccgg
     2941 tagctttagt gactcattca gatcagggat taactggtat tagtcatgaa agtcagaatt
     3001 ttgtactgaa gcacattatt ctaagtgaat tttctaaaaa agaacgtcca attctaatca
     3061 ataactggga agctacttac tttgactttc agagagaaaa actgttagag ttagcagatg
     3121 aagctaagaa agttggcatt gaactttttg tattagatga tggttggttt ggcaatcgtt
     3181 ttgatgataa tcgtgcttta ggtgattggg ttgttaatga ggaaaaactg ggtggaagtc
     3241 tagaaagtct gatttcagct atccatgaaa gaggtttgca gtttggactt tggttagaac
     3301 ccgaaatgat ttctgtagat agtgatttgt atcgtcaaca ccctgactgg gctattcagg
     3361 ttcctgacta tgagcatact tattctcgga atcaattagt acttaatctt gccaatcctc
     3421 aggtagtaga atacttgaaa agtgtcttag atcaactcct atcttatcat gagattgatt
     3481 acattaaatg ggatatgaac cgcaatatca ctaagctagg gaatggatta acttatctag
     3541 agacacagat gcaatctcat cagtacatgc tggggcttta tgaactcgtt tcttatctga
     3601 cagagaagca cagccatatt ctctttgagt cctgctctgg tggtggtgga cgaaatgatc
     3661 ttggtatgat acgctatttc ccacaagtct gggctagtga taatactgat gccattgcac
     3721 gtttaccaat tcaatacggt tcatcctatt tctatccaac catttctatg ggggctcatg
     3781 tgtcagcagt accgaatcat cagatgggac gaatgacacc attagaaaca cgtggccatg
     3841 tagcaatgat gggaaatttg ggctatgagc ttgatttgac aaatttatca gatgaagaga
     3901 aagctacgat tgctaatcag gtgaacttgt ataaagaatt acgaccagta gttcagttag
     3961 gacagcagta tagactaatt aatcctgata ctgcatccaa tgaagcagct gtacaattta
     4021 attacggaaa tcaaacgatt gtaacctacg ttcgcgtttt atctgttgta gagaccatgg
     4081 aaacaacttt aaaattaaaa gatttggatg aagagggact atataaatta caggaaaatg
     4141 gcgaagttta ctcaggtgca gaactaatgt atgcgggctt aactgttatc ttatcccaag
     4201 gagatttttt gagtagacag tatattttta gaaaactatg atatgtactg accccaaaag
     4261 ttagatttat tctatctaac ttttgaagtg cagtttgata agggtgtata aatttataaa
     4321 ggaggctttc taatggaatg gtataaaaaa atcggacttc ttgcaactac aggtttagct
     4381 ttgtttgggc tcggtgcttg ctccaactat ggtaaatctg cggatgacac agtgaccatc
     4441 gagtatttca accagaaaaa agaaatgacc aaaaccttgg aagaaatcac tcgtgatttt
     4501 gagaaggaaa accctaagat aaaggtcaaa gtcgtcaatg taccaaatgc tggtgaagta
     4561 ttgaagacac gcgttctcgc aggagatgtg cctgatgtgg tcaatattta cccacagtcc
     4621 atcgaactgc aagaatgggc aaaagcaggt gtctttgaag atttgagtaa caaagactac
     4681 ctgaaacgcg tgaaaaatgg ctacgctgaa aaatatgctg taaacgaaaa agtttacaac
     4741 gttcctttta cagctaatgc ttatggaatt tactacaaca aagataaatt cgaagaactg
     4801 ggcttgaagg ttcctgaaac ctgggatgaa tttgaacagt tagtcaaaga tatcgttgct
     4861 aaaggacaaa caccatttgg aattgcaggt gcagatgctt ggacactcaa tggttacaat
     4921 caattagcct ttgcgacagc aacaggtgga ggaaaagaag caaatcaata ccttcgttat
     4981 tctcaaccaa atgctattaa attgtcggat ccgattatga aagatgatat caaggtcatg
     5041 gacatccttc gcattaatgg ctctaagcaa aagaactggg aaggtgctgg ctataccgat
     5101 gttatcggag ccttcgcacg tggggatgtc ctcatgacac caaatgggtc ttgggcgatc
     5161 acagcgatta atgaacaaaa accgaacttt aagattggaa ccttcatgat tccaggaaaa
     5221 gaaaaaggac aaagcttaac cgttggtgcg ggagacttgg catggtctat ctcagccacc
     5281 accaaacatc caaaagaagc caatgccttt gtggaatata tgacccgttc agaagtcatg
     5341 caaaaatact acgatgtgga cggatctcca acagcgatcg aaggggtcaa acaagcagga
     5401 gaagattcac cgcttgctgg tatgaccgaa tatgccttta cggatcgtca cttggtctgg
     5461 ttgcaacaat actggaccag tgaagcagac ttccatacct tgaccatgaa ctatgtcttg
     5521 accggtgata aacaaggcat ggtcaatgat ttgaatgcct tctttaaccc gatgaaagcg
     5581 gatgtggatt aggagtagag agactatgaa aaaagtatta caaaaatatt gggcatgggc
     5641 ttttgtggtc atccccctct tgttacaagc aattttcttc tatgtgccga tgtttcaagg
     5701 agccttttac agttttacca actggacagg attgacttac aactacaaat ttgtcggctt
     5761 aaacaacttt aagctcctct tcatggatcc aaaattcatg aatgcgattg gctttaccgc
     5821 aatcattacg attgccatgg tggttggtga gattgcactc gggatcttca ttgcgcgtgt
     5881 cttgaactct aaaatcaaag gccaaacctt cttccgtgct tggttcttct tcccagctgt
     5941 tttatctggt ttgacagtgg ctttgatctt caagcaagtc ttcaactacg gtcttccagc
     6001 gattgggaat gcccttcata ttgaatttct ccaaaccagt cttttaggga ctaagtgggg
     6061 agcaatcttt gcggctgtct ttgtccttct ttggcaaggg gtggctatgc ccatcatcat
     6121 cttcctagct ggtttgcaat ctattccaac tgagattaca gaggcagcaa ggattgatgg
     6181 tgcgactagc aagcaagttt tctggaacat tgaattgcct tacttgctac caagtgtctc
     6241 tatagtcttt atcctagccc taaaaggtgg gctgactgcc tttgaccaag tctttgccat
     6301 gaccggtggt ggtccaaaca atgccacaac ctcacttggg ctcttggttt ataactatgc
     6361 ctttaaaaac aaccaattcg gttatgccaa tgccattgcc gtaatcttgt tcttcttaat
     6421 tgtagtgatt tcgatcatcc aattgagagt atctaagaaa tttgaaattt aagaggagaa
     6481 gcatgatgaa acaagatgaa agaaaagccc tgattggcaa atacattcta ttgattctag
     6541 gatcggttct gattttagtg ccgctccttg ctaccctctt tagttccttc aaatccacta
     6601 aggatattgt agataatttc tttggctttc caaccaactt cacatgggac aactttagcc
     6661 gtctcttagc tgatgggatt ggaggctatt attggaactc tgtcgtcatc actgtcttgt
     6721 ctttacttgc agtaatgatc tttatcccta tggcagccta ctccatcgct cgcaatatga
     6781 gtaaaagaaa agcctttacc atcatgtata ccctcttaat cctcggaatc ttcgtacctt
     6841 tccaagtcat catgattccg attacggtta tgatgagtaa actcggtttg gctaatacct
     6901 ttggtttgat cttgctctac ttgacctatg cgattccaca gaccctcttt ctctatgttg
     6961 gctatatcaa aatctcgatt ccagaaagtc tggatgaagc agcagagatc gatggggcta
     7021 atcaatttac aacctatttc cgcatcatct tcccaatgat gaaaccgatg catgcgacaa
     7081 ccatgatcat caatgccctt tggttctgga atgacttcat gttgccactc cttgtcttga
     7141 accgggattc caaaatgtgg actctgcctt tgttccaata caactacgca ggccaatatt
     7201 tcaacgacta cggaccaagc tttgcctctt acgtggtcgg cattatcagt atcaccattg
     7261 tctatctctt cttccaacgc catatcatcg caggaataag caacggggca gtgaagtaat
     7321 actcaatgaa aatcaaagag caaactagga agctagccgc aggttgctca aaacactgtt
     7381 ttgaggttgt ggatgaaact gacgaagtca gtaaccatat ctacggtaag gcgacactga
     7441 cgtggtttga agagattttc gaagagtata acatgaattt gatttacgaa gccagatcat
     7501 atgagacttg gcttcaatta gaaaaaggag agtaatgatg ccaattcaaa ataaaaccat
     7561 gttgattacc tattctgata gccttggaaa taatcttaaa gacttatatg ataatttgga
     7621 agagcatttt ggagatgcta ttggaggagt tcacctttta ccatttttcc catcaacagg
     7681 tgatcgtgga tttgcgccag ttgactacga cgaagtggac tcagcttttg gtgattggga
     7741 ggatgttaag cgtttaggtg agaaatatta tcttatgttt gattttatga ttaatcatat
     7801 ttctcgtcaa tccaagtatt ataaggacta tcaagaaaaa catgaagcca gtgaatttaa
     7861 agatctcttt ttaaactggg ataagttttg gccagaaaac cgtccgacac agtctgatgt
     7921 agatttaatt tacaagcgta aggatcgtgc accaaagcaa gagattgtgt ttgaagatgg
     7981 ttcagtggaa catttgtgga atacctttgg tgaggagcag attgatcttg atgtgaccaa
     8041 agaagtaact atggaattta tccgtaagac cattcagcac ttggcaagta atgggtgtga
     8101 tttgattcgt ctagatgcct ttgcttatgc agtgaagaaa ttggatacta atgatttctt
     8161 tgtggaacca gatatttggg atttattgga caaagttcga gatatcgctg ctgagtatgg
     8221 gacagagatt ttacctgaga ttcatgaaca ctattcgatt cagtttaaaa tagcagacca
     8281 tgattactat gtttatgatt ttactcttcc aatggtgaca ctttatactc tttacagttc
     8341 cagaacagag cgtttggcta agtggttgaa gatgagcccg atgaagcaat ttacgacgct
     8401 agatacccat gatgggattg gagtagtaga tgtcaaggat atcctgaccg atgaggagat
     8461 tgactatgct tcaaatgaac tctataaggt tggagccaat gtcaaacgta agtactctag
     8521 tgccgagtat aacaacttag atatctacca aatcaattca acctactatt cagcgcttgg
     8581 agatgatgat gtcaagtatt ttctcgctcg tctaattcaa gcttttgccc caggtattcc
     8641 tcaggtttac tatgtgggtc tattagcagg caagaatgac ttgaaattat tagaagaaac
     8701 taaagaaggt cgaaatatta atcgtcatta ctatagcaac gaggaaatag caagagaagt
     8761 gcaacgacct gttgtgaagg cccttctcaa tctattttct ttccgtaacc gttcagaagc
     8821 ctttgatcta gaagggacta ctgagataga gacaccaaca gctcacagca ttgtaatcaa
     8881 acgtcaaaat aaagataagt ccgtaacagc agtagcagaa attgatttgc aaaatcagac
     8941 ttatcgggta attgagaatg gagttgaagt atcattttga agccttgata tggtggataa
     9001 aatagggttt ttattttgga aaacgtttcc tttgttttca aattgctaaa aaagtggtac
     9061 aatataggat agcttactat tatctgaatc agctgatttg gagagaaagg attcattttg
     9121 aaatcaatag gctttattga aaagctgaag gggttgtcta gtaaagagct gattttattg
     9181 ggaattatcc taagtatctt tttacccttt tatctttttg tagttgtact ctgtttatat
     9241 attatcagtt tgatttttac aggagacatg aaaagtattc ttcagaaaat gggggagcat
     9301 ccgatgctgc ttctttttct tagctatagt actgttatat ccattcttgc acaaaattgg
     9361 atgggtcttg tggcttcagt aggaatgttt ctatttacta ttttcttttt gcactatcag
     9421 tcgattttat cccataaatt ctttcgattg attttgcagt tcgtcttgtt tggtagtgtc
     9481 ttgtcagctg cttttgccag tttagaacat ttccaaattg tgaagaaatt taactatgct
     9541 tttctttcac ccaatatgca ggtgtggcat cagaaccggg cagaagtgac cttctttaat
     9601 cctaattatt atggaattat ttgttgtttc tgtattatga ttgctttcta tctgtttaca
     9661 acgaccaagt tgaattggtt gaaagtattc tgtgtgattg caggctttgt taatctcttt
     9721 ggtttgaact ttactcaaaa tcgaactgcc tttcctgcta ttatcgctgg agcaattatc
     9781 tatctcttta cgactattaa aaactggaag gccttttggc ttagtattgg ggtcttcgcg
     9841 attggtttga gtttcctctt ttctagtgat ttgggagttc gaatgggtac tttagactct
     9901 tctatggaag aacgcatttc tatctgggat gctgggatgg ccttgtttaa gcaaaatcct
     9961 ttttggggtg aagggccatt gacctatatg cactcttatc ctcggataca tgctccttat
    10021 catgaacatg cccacagtct ttatattgat acgattctga gttacggaat tgtgggtacc
    10081 attttattag ttttgtcttc tgttgctcct gttcgcttga tgatggatat gagtcaggag
    10141 tcggggaaac gtccgattat cggcctttat ctatctttcc ttacagtggt tgctgtgcac
    10201 ggaatttttg acttggctct cttctggatt cagtcaggct ttattttctt gctagttatg
    10261 tgcagtattc cattggagca tcgaatgttg gtatcggaca tgacggatta agttttataa
    10321 gtttgaaatc ttctaccttg ggtaggagat ttttttatac gatgggcatg tcgtgcatct
    10381 gaggtgtaag tcctctgtag ggatccacta ccggtgaata caatagcgct ccccaagcct
    10441 gactatcgtg aggtagcgga ttaaaatggt ctggggatag atcgttttaa gtctgacgca
    10501 ggaaataaga attgtcagag aaagcaatag tgaaatcgta ggctatgaat agaaacgtga
    10561 tagcgaggag catatagtgg ataaggtagc gagaaatttt aaagtataaa agatagtctt
    10621 aatctatata tgtaaatcac aaaagtaatt gaattcggaa taaggttggt gtgaaaagac
    10681 atagtaaact gaatcttgaa tactagaatg atacttctac aatattgtta taatttaatt
    10741 tgaattccaa aatcacattg ttccgtccgt atttcatttt actgtatgtc ttcctaaact
    10801 ccaaagtttc tgcgtcaaat ttcctatttt cactgtagtc tttgacactt cgaacgacaa
    10861 tttgaatcat atattcaaaa aaaaatgaaa aatttgtgcc aacaagccta aatattctga
    10921 aaatttgttt gatttttaat ggataatgtg atataatggt tgtcaatcta taattcacat
    10981 aaacagagga gtagaaaatg aaaaaaaatc gtgtatttgc tacagcaggt cttgttttat
    11041 tagcagcagg tgtacttgca gcatgcagtt cttcaaaatc atctgattca tcagccccta
    11101 aagcttatgg ctatgtttat acagcagacc cagaaacctt ggactacctg atttcaagta
    11161 aaaatagtac aacagtagtg acttcaaatg ggattgatgg tttattcact aacgataatt
    11221 acggtaatct tgctcctgca gttgcagagg attgggaagt ctctaaggat ggtttgacct
    11281 acacttataa gattcgtaaa ggggttaaat ggtttacctc tgatggagaa gaatatgcag
    11341 aggtgacggc taaagatttc gtgaacggtt taaaacacgc agcagataaa aaatcagaag
    11401 ctatgtattt agctgaaaat tcggttaaag gcttggcaga ttatctatca ggaacttcaa
    11461 cagatttttc aacagttggt gtcaaggcgg ttgatgatta tacgttacaa tacactttga
    11521 accagcctga accgttctgg aactctaagt tgacctattc tattttctgg cctctgaatg
    11581 aagaattcga aacatcaaaa ggaagcgatt ttgctaaacc aacagatccg acatccttgc
    11641 tttataatgg tccattcttg ttgaaagggt tgactgcaaa atcttctgta gagtttgtaa
    11701 aaaatgagca atattgggat aaagaaaatg tccacctaga tactatcaat ctagcttact
    11761 atgatggatc agatcaggag tcgctagagc gtaacttcac tagtggagct tatagttatg
    11821 cccgtcttta ccctaccagc tccaactatt ctaaggttgc agaagaatac aaggacaata
    11881 tctattacac acaatcaggc tctgggattg ctggtctggg tgtgaatatt gatcgccaaa
    11941 gttacaacta tacttctaaa actacagatt cagagaaagt agctactaag aaggcattgc
    12001 ttaacaaaga tttccgtcaa gccttgaatt ttgctcttga tcgctcagct tactcagctc
    12061 aaatcaatgg taaagatgga gcagctttag cagttcgtaa tttatttgta aaaccagact
    12121 ttgtttcagc tggtgagaag acctttggtg atttagtcgc tgctcaactt cctgcttatg
    12181 gtgatgagtg gaaaggtgtg aatttagctg atgggcagga tggtttattc aatgctgaca
    12241 aggccaaggc agagtttgcg aaagctaaga aagctttaga agcagacggc gttcagtttc
    12301 ctattcatct ggacgttcca gtagaccaag catcaaaaaa ctacatatct cgtattcagt
    12361 cctttaaaca atctgtagaa acagttcttg gtgttgaaaa tgtcgttgtt gatattcaac
    12421 aaatgacaag tgatgaattc cttaatatta cttactatgc tgccaatgct tcatctgagg
    12481 attgggatgt atcgggagga gtttcatggg ggccagacta tcaagaccca tctacttacc
    12541 tggatatttt aaaaacaact agcagtgaaa ctacaaaaac atatttagga tttgataatc
    12601 caaatagccc ttcagtagtt caagttggtt tgaaagaata cgataaatta gttgatgaag
    12661 ctgccaaaga gacaagcgac ttgaatgtcc gttatgaaaa atatgcagcg gctcaagcat
    12721 ggttgacaga tagttcactc tttattcctg ctatggcttc ttctggtgca gcaccagtgc
    12781 tttcacgaat tgttccattt actggagctt ctgcgcaaac aggctctaag gggtcagatg
    12841 tttacttcaa atatttgaaa ttacaagata aagtggtgac taaggaagag tatgaaaaag
    12901 ctcgtgaaaa atggttgaaa gaaaaagctg aatcaaatga gaaagctcaa aaagaattgg
    12961 caagtcatgt gaagtaaata atttctaata gaactttctc tccatgagga gaaggttctt
    13021 ttgggatttt taaaggaaat aatatgaaaa aatatatttt tatgcgtgtt ttgcggtcat
    13081 tggtttcgat tttcttagta acgactttga cctacacgat tatctatacc ttggttcctc
    13141 gaaaattgat tttcaagcag gatcctaact ataataaaat tgcaacaacg gctgataaac
    13201 gtgataacta tgaaaatact gtgtttgagc gtatgggcta cattgagtat tacgatacta
    13261 aagagttgca agaaaaggca agtagcatgg attcttctgt aacagtagaa gcaaatgcga
    13321 ccaataaagc tatttatgaa aagtacatca atcaattagg tcatggttgg actttgggag
    13381 aatttactga aagtggtcaa ttctatgcta ctcgtgaaat tccaattttt gaacgtgttt
    13441 ttcacttcta tgctaacttg attgacattg accatacaaa taaaatccaa gaccctgaaa
    13501 atccagactt gaaacgctac cttcgttttg aaaatgatcc agctatcgga tggtcattgg
    13561 tcggttcagg aactaaacat aaatatctct tgtactttaa cagtcagttc ccatttgttc
    13621 atcaaaactt tgtgaacttg aatttaggtg actcttaccc aacctatgct aatacaccag
    13681 ttcttcaggt tattactcaa ggtcaaggac aaaccaaaac tgcccaagtt cagttcccaa
    13741 caggtaagaa aacgtcttct gtaaatattt actcaagaac ctacaagtca cctagtcagg
    13801 ctgactctcg tgaagtagct agctatggga aagatgatcc ttatacagcg actgaaagta
    13861 attaccaata tccatctatg attgtcagct ctgctattac tggtttgatt ggtttggttc
    13921 ttgcctatgc tcttgccgtg ccacttggtt cagccatggc tcgtttcaag aacacttgga
    13981 ttgatagcct ctcaacaggg gctttgacct tcttgcttgc tcttccaacg attgccttgg
    14041 tttacatcgt tcgattgatt ggatcatcta ttgcccttcc agattcattc cctatcttgg
    14101 gagctggaga ttggcgttct tacgttttac cagcagtcat ccttggtttg ttgggtgctc
    14161 ctggtacagc catttggatt cgtcgttaca tgattgactt gcaatctcaa gactttgttc
    14221 gtttcgctcg tgcaaaaggt ttgtctgaaa aagaaatttc aaacaaacac atctttaaaa
    14281 atgccatggt tccgctggtt tcaggaattc ctggtgccat cattggggtt atcggtggtg
    14341 caacccttac tgaaacagtc ttcgccttcc caggtatggg taaaatgttg attgactctg
    14401 taaaagcatc taataactct atggtcgttg gtcttgtctt catctttaca tgtatttcta
    14461 tcttctcacg tcttttggga gatatttgga tgactattat tgacccacgt attaaattga
    14521 ctgagaaagg aggcaaataa tgtctacaat cgataaagaa aaatttcagt ttgtaaaacg
    14581 tgacgatttt gcctctgaaa ctattgatgc gccagcatat tcttactgga aatcagtgtt
    14641 taaacaattt atgaagaaaa aatcaactgt agtcatgttg ggaatcttgg tagccatcat
    14701 tttgataagt ttcatctacc caatgttttc taagtttgat ttcaatgatg tcagcaaggt
    14761 aaacgacttt agtattcgtt atatcaagcc aaatgcggag cattggttcg gtactgacag
    14821 taacggtaaa tcgctctttg acggtgtctg gttcggagct cgtaactcca tcctcatttc
    14881 tgtgattgcg acagtgatta acttggttat cggtgttttt gtcggtggta tttggggtat
    14941 ttcaaaatca gttgaccgtg tcatgatgga agtttacaac gtcatctcaa acatcccacc
    15001 tcttttgatt gttattgtct tgacttactc aatcggagct ggattctgga atctgatttt
    15061 tgccatgagc gtaacaacat ggattggtat cgccttcatg atccgtgtgc aaatcttgcg
    15121 ctatcgtgac ttggaataca acttggcatc acgtactttg ggaacaccaa ccttgaagat
    15181 tgttgccaaa aatatcatgc ctcaattggt atctgttatt gtgacaacca tgactcaaat
    15241 gcttccaagc tttatctcat acgaagcctt cttgtctttc ttcggtcttg gattaccgat
    15301 tacagtgcca agtttgggtc gtttgatttc ggattattca caaaacgtaa caaccaatgc
    15361 ttacttgttc tggattccat tgacaaccct tgtcttggta tccttgtccc ttttcgtagt
    15421 tggtcaaaac ttagcggatg ctagtgatcc acgtacacat agataggagt agaaatgaca
    15481 aaagaaaaaa atgtaatttt gactgctcgc gatattgtcg tggaatttga cgttcgtgac
    15541 aaagtattga cagccattcg cggcgtttcc cttgaactcg ttgaaggaga agtattagcc
    15601 ttggtaggtg agtcaggatc aggtaaatct gttttgacaa agaccttcac aggtatgctc
    15661 gaagaaaatg gtcgcattgc ccaaggtagt attgactacc gtggtcagga tttgacagct
    15721 ttatcttctc acaaggattg ggaacaaatt cgtggtgcta agattgcgac tatcttccag
    15781 gacccaatga ctagtttgga ccccattaaa acaattggta gtcagattac agaagttatt
    15841 gtaaaacacc aaggaaaaac agctaaagaa gcgaaagaat tggccattga ctacatgaat
    15901 aaggttggca ttccagacgc agatagacgt tttaatgaat acccattcca atattctgga
    15961 ggaatgcgtc aacgtatcgt tattgctatt gcccttgcct gccgacctga tgtcttgatc
    16021 tgtgatgagc caacaactgc cttggatgta actattcaag ctcagattat tgatttgcta
    16081 aaatctttac aaaacgagta tcatttcaca acaatcttta ttacccacga ccttggtgtg
    16141 gtggcaagta ttgcggataa ggtagcggtt atgtatgcag gagaaatcgt tgagtatgga
    16201 acggttgagg aagtcttcta tgaccctcgc catccatata catggagtct cttgtctagc
    16261 ttgcctcagc ttgctgatga taaaggggat ctttactcaa tcccaggaac acctccgtca
    16321 ctttatactg acctgaaagg ggatgctttt gccttgcgtt ctgactacgc aatgcagatt
    16381 gacttcgaac aaaaagctcc tcaattctca gtatcagaga cacattgggc taaaacttgg
    16441 cttcttcatg aggatgctcc aaaagtagaa aaaccagctg tgattgcaaa tctccatgat
    16501 aagattcgtg aaaaaatggg atttgcccat ctggctgact aggaggaagg aaatgtctga
    16561 aaaattagta gaaatcaaag atttagaaat ttccttcggt gaaggaagta agaagtttgt
    16621 cgcggttaaa aatgctaact tctttatcaa caagggagaa actttctcgc ttgtaggtga
    16681 gtccggtagt gggaaaacaa ctattggtcg tgctatcatc ggtctaaatg atacaagtaa
    16741 tggagatatc atttttgatg gtcaaaagat taatggtaag aaatcgcgtg aacaagctgc
    16801 ggaattgatt cgtcgaatcc agatgatttt ccaagaccct gctgcaagtt tgaatgaacg
    16861 tgcgactgtt gattatatta tttctgaagg tctttacaat caccgtttat ttaaggatga
    16921 agaagaacgt aaagagaaag ttcaaaatat tatccgtgaa gtaggtcttc ttgctgagca
    16981 cttgactcgt taccctcatg aattctcagg cggtcaacgt caacgtatcg gtattgcccg
    17041 tgccttggtc atgcaaccag actttgttat tgcagatgag ccaatttcag ccttggacgt
    17101 ttctgtacgt gcccaagtct tgaacttgct caaaaaattc caaaaagagc tcggcctgac
    17161 ctatctcttc atcgcccatg acttgtcggt tgttcgcttt atttcagatc gtatcgcagt
    17221 tatttacaag ggtgttattg tagaggttgc agaaacagaa gaattgttta acaatccaat
    17281 tcacccatat actcaagcct tgctttcagc ggtaccaatc ccagatccaa tcttggaacg
    17341 taagaaggtc ttgaaggttt acgacccaag tcaacacgac tatgagactg ataagccatc
    17401 tatggtagaa atccgtccag gtcactatgt ttgggcgaac caagccgaat tggcacgtta
    17461 tcaaaaagga ctaaactaat aatggtttta taatttccat gtcaattttt atgggaatta
    17521 taaaactttt ttgttggact gatttgaaaa actttgaaag cttcctgaaa ga
//
LOCUS       DAVBBS010000048        16132 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVBBS010000048 DAVBBS010000000
VERSION     DAVBBS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16132
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.048"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            145..6831
                     /locus_tag="VQU32_001577"
     CDS             145..6831
                     /locus_tag="VQU32_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET2473007.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTVEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQTSNQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDVDVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGFKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVARVRTLIGKEPKMPKTVGFVYSDGSREKLPVTWSQ
                     VDVSQAGVVTVKGTANGREVEAHVEVLAIAKELPTVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRIEQYA
                     KEGVFTVNGRLEDTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQIRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLRFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGKERIFT
                     AINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSEASQTN
                     KAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(7079..7935)
                     /locus_tag="VQU32_001578"
                     /pseudo
     CDS             complement(7079..7935)
                     /locus_tag="VQU32_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8146..8505)
                     /locus_tag="VQU32_001579"
     CDS             complement(8146..8505)
                     /locus_tag="VQU32_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HET2473008.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            8597..9553
                     /locus_tag="VQU32_001580"
                     /pseudo
     CDS             8597..9553
                     /locus_tag="VQU32_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            9537..10094
                     /locus_tag="VQU32_001581"
     CDS             9537..10094
                     /locus_tag="VQU32_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2473009.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQADLTNAQLILQGHETLDQFVIKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            10087..10341
                     /locus_tag="VQU32_001582"
     CDS             10087..10341
                     /locus_tag="VQU32_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473010.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERSVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            10353..12407
                     /locus_tag="VQU32_001583"
     CDS             10353..12407
                     /locus_tag="VQU32_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET2473011.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            12586..13446
                     /locus_tag="VQU32_001584"
     CDS             12586..13446
                     /locus_tag="VQU32_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET2473012.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLCFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            13657..14367
                     /gene="ccdA2"
                     /locus_tag="VQU32_001585"
     CDS             13657..14367
                     /gene="ccdA2"
                     /locus_tag="VQU32_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET2473013.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            14387..14953
                     /locus_tag="VQU32_001586"
     CDS             14387..14953
                     /locus_tag="VQU32_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HET2473014.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            14964..16076
                     /gene="msrB"
                     /locus_tag="VQU32_001587"
     CDS             14964..16076
                     /gene="msrB"
                     /locus_tag="VQU32_001587"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET2473015.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
BASE COUNT         4901 a         3052 c         3565 g         4614 t
ORIGIN      
        1 catatctacg gcaaggcgac actgacgaag tttgaattgg attttcgcag agtgtaaatt
       61 gttgaaacta ggttgtcata ccatgtatac cacttgggct ttggtagtag ctaactgcac
      121 taaatataat ataaggaggg ttttatgggg aaaggccatt ggaatcggaa aagagtttat
      181 agcattcgta agtttgctgt gggagcttgc tcagtaatga ttgggacttg tgcagttcta
      241 ttaggaggaa atatagctgg agaatctgta gtttatgcgg atgaaacact tattactcat
      301 actgttgaga aacctaaaga ggaaaaaatg atagtagaag aaaaggctga taaagctttg
      361 gaaactaaaa atgtagttga aaggacagaa caaagtgaac ctagttcaac tgaggctatt
      421 gcatctgaga agaaagaaga tgaagccgta actccaaaag aggaaaaagt gtctgctaaa
      481 ccggaagaaa aagctccaag gatagaatca caaacttcaa atcaagaaaa accgctcaag
      541 gaagatgcta aagctgtaac aaatgaagaa gtgaatcaaa tgattgaaga caggaaagtg
      601 gattttaatc aaaattggta ttttaaactc aatgcaaatt ctaaggaagc cattaaacct
      661 gatgtagacg tatctacgtg gaaaaaatta gatttaccgt atgactggag tatctttaac
      721 gatttcgatc atgaatctcc tgcacaaaat gaaggtggac agctcaacgg tggggaagct
      781 tggtatcgca agactttcaa actagatgaa aaagacctca agaaaaatgt tcgccttact
      841 tttgatggcg tctacatgga ttctcaagtt tatgtcaatg gtcagttagt ggggcattat
      901 ccaaatggtt ataaccagtt ctcatatgat atcaccaaat accttcacaa agatggtcgt
      961 gagaatgtga ttgctgtcca tgcagtcaac aaacagccaa gtagccgttg gtattcagga
     1021 agtggtatct atcgtgatgt gactttacaa gtgacagata aggtgcatgt tgagaaaaat
     1081 gggacaacta ttttaacacc aaaacttgaa gaacaacaac atggcaaggt tgaaactcat
     1141 gtgaccagca aaatcgtcaa tacggacgac aaagaccatg aacttgtagc cgaatatcaa
     1201 atcgttgaac gaggtggtca tgctgtaaca ggcttagttc gtacagcgag tcgtacctta
     1261 aaagcacatg aatcaacaag cctagatgcg attttagaag ttgaaagacc aaaactctgg
     1321 accgttttaa atgacaaacc tgccttgtac gaattgatta cgcgtgttta ccgtgacggt
     1381 caattggttg atgctaagaa ggatttgttt ggttaccgtt actatcactg gactccaaat
     1441 gaaggtttct ctttgaatgg tgaacgtatt aaattccatg gagtatcctt gcaccacgac
     1501 catggggcgc ttggagcaga agaaaactat aaagcagaat atcgccgtct caaacaaatg
     1561 aaggagatgg gagttaactc catccgtaca acccacaacc ctgctagtga gcaaaccttg
     1621 caaatcgcag cagaactagg tttactcgtt caggaagagg cctttgatac ttggtatggt
     1681 ggcaagaaac cttatgacta tggacgtttc tttgaaaaag atgccactca cccagaagct
     1741 cgaaaaggtg aaaaatggtc tgattttgac ctacgtacca tggtcgaaag aggcaaaaac
     1801 aaccctgcta tcttcatgtg gtcaattggt aatgaaatag gtgaagctaa tggtgatgcc
     1861 cactctttag caactgttaa acgtttggtc aaggttatca aggatgttga taagactcgc
     1921 tatgttacca tgggagcaga taaattccgt ttcggtaatg gtagcggagg gcatgagaaa
     1981 attgctgatg aactcgatgc tgttggattt aactattctg aagataatta caaagccctt
     2041 agagctaagc atccaaaatg gttgatttac ggttcagaaa catcttcagc tacccgtaca
     2101 cgtggaagtt actatcgccc tgaacgtgaa ttgaaacata gcaatggacc tgagcgtaat
     2161 tatgaacagt ctgactatgg aaatgatcgt gtgggttggg ggaaaacagc aaccgcttca
     2221 tggacttttg accgtgacaa cgctggctat gctggacagt ttatctggac aggtacggac
     2281 tatattggtg aacctacacc atggcacaac caaaatcaaa ctcctgttaa gagctcttac
     2341 tttggtatcg tagatacagc cggcattcca aaacatgact tctatctcta ccaaagccaa
     2401 tgggtttctg ttaagaagaa accgatggta caccttcttc ctcactggaa ctgggaaaac
     2461 aaagaattag catccaaagt agctgactca gaaggtaaga ttccagttcg tgcttattcg
     2521 aatgcttcta gtgtagaatt attcttgaat ggaaaatctc ttggttttaa gactttcaat
     2581 aaaaaacaaa ccagcgatgg gcggacttac caagaaggtg caaatgctaa tgaactttat
     2641 cttgaatgga aagttgccta tcaaccgggt accttggaag caattgctcg tgatgaatct
     2701 ggcaaggaaa ttgctcgaga taagattacg actgctggta agccagcggc agttcgtctt
     2761 attaaggaag accatgcgat tgcagcagat ggaaaagact tgacttacat ctactatgaa
     2821 attgttgaca gccaggggaa tgtggttcca actgctaata atctggttcg cttccaattg
     2881 catggccaag gtcaactggt cggtgtagat aacggagaac aagccagccg tgaacgctat
     2941 aaggcgcaag cagatggttc ttggattcgt aaagcattta atggtaaagg tgttgccatt
     3001 gtcaaatcaa ctgaacaagc agggaaattc accctgactg cccactctga tctcttgaaa
     3061 tcgaaccaag tcactgtctt tactggtaaa aaagaaggac aagagaagac tgttttgggg
     3121 acagaagtag caagagttcg tacattaatt ggtaaagaac caaaaatgcc gaaaacagta
     3181 ggttttgtat acagcgatgg tagtcgtgaa aaacttccag taacttggtc tcaagtcgat
     3241 gttagtcaag caggagttgt gactgttaaa ggtacagcaa atggacgtga ggtagaggct
     3301 catgtcgagg ttctagcaat tgcgaaagaa ttaccaacag ttaaacgtat tgctccaaat
     3361 actgacttga attctgtaga caaatctgtt tcttatgttt tgactgatgg aagtgtacaa
     3421 gagtacgaag tagatagctg ggagattacg gaagtagata aagccaaact ttcagtagct
     3481 ggatcacgta ttcaaatgac tggtcagtta gctggagaaa ctattcatgc aacccttgtg
     3541 gtagaagaag gaaatgctgc agcacctgta gtgccaactg ttactgttgg aggtgaggca
     3601 gtaacaggtc ttactagtcg acaaccaatg caatatcgta ctctatctta tggtgcccaa
     3661 ctgccagaag tcacagcaag tgctgaaaat gctgatgtga cagttcttca agcaagcgca
     3721 gcaaacggca tgcgtgcgag catctttatt cagcctaaag atggtggtcc tcttcaaacc
     3781 tatgcaattc aattcctaga agaagcacca aaaattgctc acttgagttt gcaagtggaa
     3841 aaagctgaca gtctcaaaga agaccaaact gtcaaattgt cggttcgagc tcactatcaa
     3901 gatggaacgc aagctgtatt accagctgac aaagtaacct tctctacaag tggtgaaggg
     3961 gaagtcgcaa ttcgtaaagg aatgcttgag ttgcataagc caggagcagt cactctcaaa
     4021 gcggaatatg agggagctaa aggccaagtt gaactcacta tccaagccaa tactgagaag
     4081 aagattgcgc aatctatccg tcctgtaaat gtagtcacag atttgcatca aaaacctact
     4141 cttccaacaa cagtaacggt tgagtatgac aaaggtttcc ctaaagctca caaagtcact
     4201 tggcaagcta ttccgaaaga aaaactagac tcctatcaaa catttgaagt actaggtaaa
     4261 gttgaaggaa ttgaccttga agcgcgtgca aaagtctctg tagaaggtat cgtttcagtt
     4321 gaagaagtca gtgtgacaac tccaatcgca gaagcaccac aattaccaga aagcgttcgg
     4381 acatatgatt caaatggtca cgtttcatca gctaaggttg catgggatgc gattcgtata
     4441 gagcaatacg ctaaggaagg tgtctttaca gttaatggtc gcttagaaga tactcaatta
     4501 acaactaaac ttcatgttcg cgtatctgct caaactgagc aaggtgcaaa catttctgac
     4561 caatggaccg gttcagaatt gccacttgcc tttgcttcag attcaaatcc aagcgaccca
     4621 gtttcaaatg ttaatgacaa gctcatttcc tacaataacc aaccagccaa tcgttggaca
     4681 aactggaatc gtactaatcc agaagcttca gtcggtgttc tgtttggaga ttcaggtatc
     4741 ttgagcaaac gctccgttga taatctaagt gtcggattcc acgaagacca tggagttggt
     4801 gcaccgaagt cttatgtgat tgagtattat gttggtaaga ctgtcccaac agctcctaaa
     4861 aaccctagtt ttgttggtaa tgaggaccat gtctttaatg attctgccaa ctggaaacca
     4921 gttactaatc taaaagcccc tgctcaactc aaggctggag aaatgaacca ctttagcttt
     4981 gataaagttg aaacctatgc tgttcgtatt cgcatggtta aagcagataa caagcgtgga
     5041 acgtctatca cagaggtaca aatctttgcg aaacaagttg cggcagccaa acaaggacaa
     5101 ataagaatcc aagttgacgg caaagactta gcaaacttca accctgattt gacagactac
     5161 taccttgagt ctgtagatgg aaaagttccg gcagtcacag caagtgttag caacaatggt
     5221 ctcgctaccg tcgttccaag cgttcgtgaa ggtgagccag ttcgtgtcat cgcgaaagct
     5281 gaaaatggcg acatcttagg agaataccgt ctgcacttca ctaaggataa gaacttactt
     5341 tctcataaac cagttgctgc ggttaaacaa gctcgcttgc tacaagtagg tcaagcactt
     5401 gaattgccga ctaaggttcc agtttacttc acaggtaaag acggctacga aacaaaagac
     5461 ctgacagttg aatgggaaga agttccagca gaaaatctga caaaagcagg tcaatttacc
     5521 gttcgaggcc atgtccttgg tagtgatctt gttgctgagg tcactgtacg agtgacagac
     5581 aaactaggcg aagctctttc agataaccct aactatgatg aaaacagtaa ccaggccttt
     5641 gcttcagcaa ccaatgatat tgacaaaaac tctcatgacc gcgttgacta tctcaatgac
     5701 ggagatcatt cagaaaatcg tcgttggaca aactggtctc caacaccatc ttctaatcca
     5761 gaagtatcag cgggtgtgat cttccgtgaa aatggtaaga ttgtagaacg gactgttgcc
     5821 caagccaaac ttcgtttctt tgcagatagt ggtacggatg caccaactaa actcgtttta
     5881 gaacgctatg tcggtccaga gtttgaagtg ccaacctact attcaaacta ccaagcctac
     5941 gacgcagacc atccattcaa caatccagaa aattgggaag ctgtgcctta tcgtgcggat
     6001 aaagacatcg cagctggtga tgaaatcaac gtaacattta aagctgtcaa agccaaagct
     6061 atgagatggc gtatggagcg taaagctgac aagagcggtg ttgcgatgat tgagatgacc
     6121 ttccttgcac cgagtgaatt gcctcaagaa agtacgcaat cgaagattct tgtagatgga
     6181 aaagaacttg ctgatttcgc tgaaaatcgt caagactatc aaattaccta taaaggtcaa
     6241 cggccaaaag tctcagttga agaaaacaat caagtagctt caactgtggt agatagtgga
     6301 gaagatagcc ttccagtact tgttcgcctc gtttcagaaa gtggaaaaca agtcaaggaa
     6361 taccgtatcc agttgactaa ggaaaaacca gtttctgctg tacaagaaga tcttccaaaa
     6421 ctcgaatttg ttgaaaaaga tttggcctac aagacagttg agaaaaaaga ttcaacactg
     6481 tatctaggtg aaactcgtgt agaacaagaa ggaaaaactg gtaaagaacg tatctttaca
     6541 gcgattaatc ctgatggaag taaggaagaa aaactccgtg aagtggtaga agctccgaca
     6601 gaccgcatag tcttggttgg aaccaaacca gtagctcaag aagctaaaaa accacaagtg
     6661 tcagaaaaag cagatacaaa accaattgat tcaagtgaag ctagtcaaac taataaagcc
     6721 cagttaccaa atacaggtag tgcggcaagc caagcagcag tagcagcagg tttagctctt
     6781 ctaggtttga gtgcaggatt agtagttact aaaggtaaaa aagaagacta gaaattttga
     6841 taccttcttt atcattaaag accaaagaaa aactcttgtc ctaaatagaa gataaagagt
     6901 ctggaacaaa atagttctca accacaaaaa agctagatat ttccaattgc ggaactctag
     6961 ctttttaatt ttgagtcgtt ctcttattgt atagtagatt gaaactagaa tagtacactt
     7021 ctacttctaa aacattgtta gaaatcaatt tgactgtcct tatcgatttg tcctgttctt
     7081 atttcatttc actatgtttt aggaggttat tggccataag taccaatccc atgtcaattc
     7141 tcacttgacg cttccctctc agattacatc tcttgtaacc caaacaagcc tttatctgcc
     7201 caaagacagg ttccacatca atcttgcgtt gagcgaaaat ctgtctacct tggggagata
     7261 aaagcgcctg acattcttta gctttcaagt tttgatagcg ttcgttcata tacagtccct
     7321 tttgagggac tgattcaggt tcatcagcgt agtaaacctt gatttcctgt tgaaagtctg
     7381 tctgtgtttt ctggtacttg atatggtgaa aacgatagca ccagccatca ggatgtgtgt
     7441 agctatcctc cttgtcatta tagtgccaat tcgctaagtt tttagctgac tgtttatacc
     7501 ctctcttttg ttccttatca aacatggcat atttaatcag aaggggagtg atttcttctt
     7561 gaaaatagac ctgtatctgt tcttgaagtt tggcggagaa tttctctgtc gttttcttcc
     7621 acacaaaact atacttgttg gcattggctt caatcttagt tccgtcaata aacaaacaat
     7681 ctaaggtcac taactcttcc atttttcaat ttttcgacca gagaaaatcc cttgtgaata
     7741 ggcaaataga agagtagata caagcatttt agggtgataa gacgggcgat caaaggcatg
     7801 atagaaggcg tggaagtgac aatcctccaa ggtattcacc actttttcaa tagtaaaaac
     7861 gagatgatct tgtggcaaga aggaactgat ttctagtggt aaagttgttt gatttgtgtt
     7921 atagtgaata tgcatgggat agcctttcta aatggtttat taagcaattc tattttacca
     7981 agactatcgc aaccatgcaa aaaagcaacg gcaaatccgt tgcctttttt ggagataaga
     8041 gactattgtc ccagactctt ttctttgtat aaaattgtat agtgttattg tctatactat
     8101 tttgtagaga aagcaaaaat ccctagaaaa tgcttaggga ttcgactatt tcattaagaa
     8161 caattcaaca atcatttcta tatacatgat aagggttaat aagaatcctg cacgccagaa
     8221 gaatttgata aatttaggat agtaaaagct gcgttttttc aaaaggaaga aaaagacgag
     8281 aataatggct aggatcgaga gggctagccc cagtctaggg aggaaattat gggtaaaggt
     8341 tttagctgta atcaagtaat actcaaatac caagactgga aaagccaaat ccgcaaagtt
     8401 tcgtcctagt ttttttaatc taaaaagttt ggttaggata atgcagacga ctaaggtcag
     8461 tatcaataat aaaatagatg ctaatttcat taaaatcata cccatattgt atcataaaaa
     8521 aaggctaatg gaaacgagaa acagaggaat ttataggaaa gtcaggcttt tgagattgtg
     8581 ggtgattttt gttataatga aagttataaa atcttataat accttgaatg attattatcg
     8641 aaaactcttt ggagaaaaga cttttaaagt ccctattgat gcgggatttg actgtcccaa
     8701 tcgtgatgga actgtggctc atggaggctg tactttttgt acggtttccg gttctggaga
     8761 tgccattgtg gcaccagatg cgcctatccg tgagcaattt tataaggaaa ttgactttat
     8821 tcatcgtaag tggccagatg ttcagaagta tctggtttat tttcaaaatt ttaccaacac
     8881 ccatgaaaag gtggaagtta ttcgagagcg ttatgaacag gctatcaatg agccaggtgt
     8941 ggtaggaatc aatattggaa cgcgcccaga ctgtttacca gacgaaacca tcgaatattt
     9001 ggctgagtta tcggagtgca tgcatgtgac ggttgaattg ggcttgcaaa ctacttatga
     9061 agcaacctct gacctgatta accgtgcgca ttcttatgaa ttgtaggtgg aaacggtcaa
     9121 gcgtttgaga aagtatccaa aaattgagat tgtttcccat ttgatcaatg gtttgcctgg
     9181 tgaaacccat gagatgatgg ttgaaaatgt ccgccgttgt gtcacggata acgatattca
     9241 agggattaaa ctgcatttgc tccatcttat gacaaatact cgtatgcaac gagattacca
     9301 tgaggggcgt ttacaattga tgagtcagga cgaatatgtc agggtcatct gtgaccaact
     9361 ggaaattatt cccaaacata tcgtcatcca tcgaattaca ggagatgcgc ctagggatat
     9421 gctgcttggg cctatgtgga gcctcaataa atgggaagct ctcaacagca ttgagatgga
     9481 gatgcgacgt cgtggaagtg ttcaaggatg caaggctgta aaacaggagt ttgaaaatga
     9541 aaagaccact tgagatggca catgattttt tggctgaggt cgtgacaaaa gaggatgtcg
     9601 tagtggatgc gactatggga aatggtcatg acacgctttt tttagccaag ctagccaagc
     9661 aagtctatgc ctttgatatt cagaagcaag ccttggaaaa gacccaagag cgtttgcatc
     9721 aggctgactt gacaaatgcc cagttaatct tgcaaggcca tgagacactg gaccagtttg
     9781 tgataaaagc taaggcaggg atttttaatc tgggctattt gccgtcagct gataagtctg
     9841 tcatcacccg accgcagaca acgattgagg cattagaaaa gctatgtggc ttacttgtca
     9901 aaggtggacg aattgctatt atgatttact atggtcatga aggaggcgat ctcgagagag
     9961 atgctgtctt ggattttgtg atccagttga accaacaaga gtacacagct gccatttacc
    10021 gaactttaaa ccaagtcaac aacccgccgt ttttagtgat gattgaaaaa ttagagagat
    10081 acagacatgg ataaacaata cctacacgaa aagctggatg ccatgcgcca gaattttgtt
    10141 gaatcaaccc accacgaacg atcggtgggc gtgctagacc aagcgcatat gagcaaaaaa
    10201 atgcttaaaa tcaagaaaaa attagttgct cttgaaatgg aacggtgcca gagaaaaatt
    10261 gagcacaaag actgttccaa gattgaccaa aaaatcaaag agcagaagga gatatttgaa
    10321 tcctgttgta aaaaagatta aggaggacgt gcgtggaatt actgatttac ctcatcctat
    10381 ttttactggt tttgattgtc tcaagtacaa ccaataagct cctgcccttt ttgcctctcc
    10441 ctttggtgca gattcttttg ggaattgtga ttggtctctt tttacccaat actgactttc
    10501 accttaatac ggagttgttt ttggcactgg ttatcggacc cttgcttttc cgagaggctg
    10561 aagaagcaga tgttacggct attttaaaac actggcgaat cattgtttat ctcatatttc
    10621 cagtgatttt tatctcgacc ttgagtttgg gtggcttggc ccatcttctt tggttcagcc
    10681 ttcccttggc agcttgcttg gctgttgggg cagcccttgg tcctacggac ttggtggcct
    10741 ttgcctctct ttcggagcgt tttagctttc ctaagcgcgt gtccaatatt cttaagggcg
    10801 aaggactctt gaatgatgct tctggtttgg tggcttttca ggtagctttg acagcttgga
    10861 caactggagc tttttctctg gggcaagcta gcagttcgct catcttttca atcctaggcg
    10921 gttttttaat tggattttta acggccatga ccaaccgctt cctccatacc ttcttgctaa
    10981 gtgtgcgcgc aacggatatt gccagtgaac ttttattaga attgagtttg cctctagtga
    11041 ccttctttct ggcagaagaa gttcatgttt caggtattat tgccgtcgta gttgctggaa
    11101 ttttaaaggc aagtcgcttc aagaaaatca cgctcctcga agcccaagtg gatacggtga
    11161 ccgagacggt ctggcataca gtgaccttta tgctcaacgg ttctgtcttt gtgattttag
    11221 ggatggagtt ggaaatgata gcagaaccta tcttgaccaa tccaatctat aatcctctac
    11281 ttttattgct atctctcatc gcccttacct ttgtcctctt tgtcattcgt tttattatga
    11341 tctatggcta ttatgcctat agaacccgac gcctcaagaa aaagctaaat aagtatatga
    11401 aggacatgtt tctcttgacc ttttcaggtg ttaagggaac ggtgtcgatt gctacgattc
    11461 tcttgatacc aagtaatcta gaacaggagt atcctctttt gcttttcctt gttgcaggtg
    11521 tgacgcttgt cagcttttta acaggtctct tggtcttgcc tcatctttct gatgaagagg
    11581 aagaaagcaa ggattatctc atgcatatcg ccattttgaa tgaagtaacg ctagagttgg
    11641 aaaaagagtt ggaagacacc agaaataaac ttcccctcta tgcggctatt gacaattatc
    11701 atggacgtat tgaaaatctc attttaagcc aagaaaacca ggatgatcaa gaagattggg
    11761 ctgctttgaa actcttgatt cttagtattg aaagtgatgg tttggaacag gcctatgaag
    11821 aggggaacat tagcaatcgt gcttaccgag tttaccaacg ttatctgaaa aatatagaac
    11881 aaggaatcaa tcgtaaactt gcctcaagac tgacctatta ttttcttgtt tccttgagga
    11941 ttttacgttt tctccttcat gaagttttta ctcttggaaa gaccttccgt agctggaagg
    12001 acaaggagca aagccgtctc cgtgctcttg attatgacca aattgcagag ctctatcttg
    12061 ccaatacaga gatgattatt gaaagtttgg aaaacctgaa gggagtctac agacgctctt
    12121 tgattagttt tatgcaggag tctcgtcttc gagaaacagc tattatcagc agtggtgcct
    12181 ttgtcgaacg ggttatcaat cgtgtcaaac ccaacaatat cgatgaaatg ctgagaggct
    12241 attatctgga gcgcaagttg attttcgaat acgaagaaaa acgattgatt acgactaagt
    12301 atgccaagaa attacgacaa aatgtaaata acttagagaa ctattccttg aaggaagctg
    12361 ccaataccct gccgtatgat atggtggaat tggtaagaag aaattagtta atactcttcg
    12421 aaaatctctt caaaccacgt cagcgtcgcc ttggattata tatgtgactg acttcgtcag
    12481 tttcatctac aacctcaaag cagggctttg agcaacctgc ggctagcttc ctagtttgct
    12541 ctttgatttt cattgagtat aagattataa atgaaggagt atgacatgaa aaagtggtgg
    12601 aaagagctga tagacaagcc tttattaaaa gcttttttgc attattatca agcatcagat
    12661 agtgagttga ccagtgtcgc agtagcctac tattggttga tttcgatttt ccccctgctt
    12721 ttggtggtgg tcaatatcct cccctatttt cagattcctg tgggagaatt tctgtgcttt
    12781 atgaaagacg ttctgccccc aagcctctat gaaggtgtgg aaaaaatagc cagagaagtt
    12841 ttgacgcaac cttcaacagg tttattgagt ttttctgttt tatcggcgtt atggagtttt
    12901 tcaaaatcta tgaatttctt gcaaaaagct tttaacaagg cttatggcgt cgaaaagagt
    12961 cgtggactta tttcccatca gatgctaagt ctcctagtca gttttggctt gcagctcctc
    13021 tttgcgtttg ccttgttttt gatcttattt ggtcagatga ttttggcttt acttgctcat
    13081 tattggacaa aagatggcat tatctatcag gctttacaag ggttggcagg tcctctgatt
    13141 tacgccctcc tctttgctat cttggtcatg ctttattatt ttcttcccaa tctatctaac
    13201 aggaaaatta gctataccct accaggcagt gcctttgttc tcttagtgat tttaggttta
    13261 ctaactctgt tctctagtta tctcaattac tatgtccatc atttggtgga tgtccgaatt
    13321 ttaggttccg ttcttcttgt tgttatgatg ttttggttta tcttgattgc taaaattgtt
    13381 atcctaggcg cggttatcaa tgccagtatg cagagtttga aagatccggt ctttaaaaaa
    13441 gactaactat ttatccatta caaaagcgca tagtatcaaa actttataga accttgatac
    13501 tatgcgcttt tgtgctttga cagttttttg tatagttttt gtccaagatt tctaaatcat
    13561 ctgattaaat tcctattttc ccctatcatt gtccctgttt tttcgaaaat ggaaggagta
    13621 taataaatct atcaataaaa tgataggagg aactttatgg gtcatatttt cttttttcta
    13681 agtgtctttt tggcagggat tctatccttc ttttctcctt gtatcttacc tttgttaccg
    13741 gtctatacag gagtgttact agatgataag gatggtgctc aggcttctag cggcaaattt
    13801 tcaatctcag ttactagttt attacgaact ctggccttta tagcaggaat ttcctttata
    13861 tttattttgt tgggctatgg agctggtttt ttaggcgatt tgctttatgc ttcttggttc
    13921 caatatctta ctggggcaat tattatcctt cttggtttgc accaaatgga gattctacac
    13981 tttaaggggc tttataagga aaagaggcta caactgcaag gacaggggca aaatggtaag
    14041 ggctatagtc aggcattttt attgggcttg acctttagtt ttgcttggac gccttgcgtg
    14101 gggccggttc tggggtctgt tttggccttg gcggcttcag gtggttcagg agcttggcag
    14161 ggagctggtc tcatgttggt gtatacgctg ggcttggcgc taccattctt gcttctagct
    14221 ctgacctcta gttatgtttt gaaacatttc cgaaaacttc atccctatct cggaatcctc
    14281 aaaaaagtgg gtggttttct cattattgtg atgggactct tggttctgtt tggaaatgct
    14341 tcaattttaa gtcaattatt tgaataaaat ggaaaggaat atcaatatga aaaaatggca
    14401 aacatgtgtt cttggagcag gttcgctcct ttgtttgacg gcttgttcag gcaagtccgt
    14461 gactagtgaa caccaaacga aagatgaaat gaagacggag cagacagcta gtaaaacaag
    14521 cgcagctaaa gggaaagagg tggctgattt tgaattgatg ggagtagatg gcaagaccta
    14581 ccgtttatct gattacaagg gcaagaaagt ctatctcaaa ttctgggctt cttggtgttc
    14641 catctgtctg gctagtcttc cagatacgga tgagattgct aaagaagctg gtgatgacta
    14701 tgtggtcttg acagtagtgt caccaggaca taagggagag caatctgaag cggactttaa
    14761 gaattggtat aagggattgg attataaaaa tctcccagtc ctagttgacc catcaggcaa
    14821 acttttggaa acttatggtg tccgttctta cccaacccaa gcctttatag acaaagaagg
    14881 caagctggtc aaaacacatc caggattcat ggaaaaagat gcaattttgc aaactttgaa
    14941 ggaattagcc taggaggtgt cttatgaatg ataagttaaa aatcttcttg ttgctaggag
    15001 tattttttct agccataacc ggtttctatg ttctattgat acgaaatgca gggcagacag
    15061 atgcctcgca aattgaaaag gcggcagtta gccaaggagg aaaagcagtg aaaaaaacag
    15121 aaattagtaa agacgcagac ttgcacgaaa tttatctagc tggaggttgt ttctggggag
    15181 tggaggaata tttctcacgc gttcccgggg tgacggatgc cgtttcaggc tatgcaaatg
    15241 gtagaggaga aacaaccaag tacgaattga ttaaccaaac aggtcatgca gaaaccgtcc
    15301 atgtcaccta tgatgccaag caaatttctc tcaaggaaat cctgcttcac tatttccgca
    15361 ttatcaatcc aaccagcaaa aataaacaag gaaatgatgt ggggacccag taccgtactg
    15421 gtgtttatta cacagatgac aaggatttgg aagtgattaa ccaagtcttt gatgaggtgg
    15481 ctaagaaata cgatcaacct ctagcagttg aaaaggaaaa cttgaagaat tttgtggtgg
    15541 ctgaggatta ccatcaagac tatctcaaga aaaatccaaa tggctactgc catatcaatg
    15601 ttaatcaggc ggcctatcct gtcattgatg ccagcaaata tccaaaacca agtgatgagg
    15661 aattgaaaaa gaccctgtca cctgaggagt atgcagttac ccaggaaaat caaacagaac
    15721 gagctttctc aaaccgttac tgggataaat ttgaatccgg tatctatgtg gatatagcaa
    15781 ctggggaacc tctcttttca tcaaaagaca aatttgagtc tggttgtggc tggcctagtt
    15841 ttacccaacc catcagtcca gatgttgtca cctacaagga agataagtcc tacaatatga
    15901 cgcgtatgga agtgcggagc cgagtaggag attctcacct tgggcatgtc tttacggatg
    15961 gtccacagga caagggcggc ttacgttact gtatcaatag cctctctatc cgctttattc
    16021 ccaaagacca aatggaagaa aaaggctacg cttatttact agattatgtt gattaatact
    16081 cttcgaaaat ctcttcaaac cacgtcagcg tcgccttacc gtatgtaggg tt
//
LOCUS       DAVBBS010000049        15156 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVBBS010000049 DAVBBS010000000
VERSION     DAVBBS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15156
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.049"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            369..2087
                     /locus_tag="VQU32_001588"
     CDS             369..2087
                     /locus_tag="VQU32_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HET2473016.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPKEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            2198..2545
                     /locus_tag="VQU32_001589"
     CDS             2198..2545
                     /locus_tag="VQU32_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET2473017.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(2823..3659)
                     /locus_tag="VQU32_001590"
     CDS             complement(2823..3659)
                     /locus_tag="VQU32_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2473018.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(3672..4301)
                     /locus_tag="VQU32_001591"
     CDS             complement(3672..4301)
                     /locus_tag="VQU32_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2473019.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLDNLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(4311..4952)
                     /locus_tag="VQU32_001592"
     CDS             complement(4311..4952)
                     /locus_tag="VQU32_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2473020.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(5098..6327)
                     /locus_tag="VQU32_001593"
     CDS             complement(5098..6327)
                     /locus_tag="VQU32_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET2473021.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEESENLLT
                     KWMIARSYQEMDDLDTAYEHYQELIGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(6317..7483)
                     /locus_tag="VQU32_001594"
     CDS             complement(6317..7483)
                     /locus_tag="VQU32_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET2473022.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            7627..8640
                     /locus_tag="VQU32_001595"
     CDS             7627..8640
                     /locus_tag="VQU32_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HET2473023.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(8685..9104)
                     /locus_tag="VQU32_001596"
     CDS             complement(8685..9104)
                     /locus_tag="VQU32_001596"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET2473024.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDQVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(9115..10521)
                     /gene="atpD"
                     /locus_tag="VQU32_001597"
     CDS             complement(9115..10521)
                     /gene="atpD"
                     /locus_tag="VQU32_001597"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET2473025.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFAGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(10607..11485)
                     /locus_tag="VQU32_001598"
     CDS             complement(10607..11485)
                     /locus_tag="VQU32_001598"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET2473026.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(11501..13006)
                     /locus_tag="VQU32_001599"
     CDS             complement(11501..13006)
                     /locus_tag="VQU32_001599"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET2473027.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALIHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(13021..13557)
                     /locus_tag="VQU32_001600"
     CDS             complement(13021..13557)
                     /locus_tag="VQU32_001600"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HET2473028.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(13557..14051)
                     /gene="atpF"
                     /locus_tag="VQU32_001601"
     CDS             complement(13557..14051)
                     /gene="atpF"
                     /locus_tag="VQU32_001601"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET2473029.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(14065..14781)
                     /gene="atpB"
                     /locus_tag="VQU32_001602"
     CDS             complement(14065..14781)
                     /gene="atpB"
                     /locus_tag="VQU32_001602"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET2473030.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(14816..15016)
                     /locus_tag="VQU32_001603"
     CDS             complement(14816..15016)
                     /locus_tag="VQU32_001603"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HET2473031.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
BASE COUNT         4661 a         3348 c         2745 g         4402 t
ORIGIN      
        1 ttgttcacgt ctttcttttc ccacgtgaag agagttctga cgccaacacc aaaaaacttt
       61 ggccgcctcg acatgtctgt gccctccttt gatggaatct aatgctcctt gtttaaaatc
      121 agtattatat gccatagctt tattagacca tgcttatttt aaactaagca actatatcaa
      181 tgttttgtta tgagaagact tacttttttt actgaaattg aatttttcct agcctcaaca
      241 tcatataacc ccaaaattca atctgacctg gtttgaagag atgttcgaag cagataaggt
      301 tttactaaaa ctaaaaaaaa tgatataatc aaactgatta aatgttttaa aaataaagga
      361 gaatttgaat gtcttaccaa gaaaattacc agaaatgggt tgattttgtg gagcttcctg
      421 actaccttcg tcaagatttg gaaaatatgg acgaaaaaac taaggaagat gccttctata
      481 caaatcttga atttggtact gcaggtatgc gtggcttggt tggtgctggt acaaaccgta
      541 tcaacatcta cgttgttcgc caagctactg aaggattggc tcgtttgatt gagtcaaaag
      601 gtggaaacga gaaagaacgc ggtgtagcaa ttgcctacga tagccgtcac ttctcacctg
      661 agtttgcctt tgaatctgcg gcagttcttg ctaaacacgg catcaaatct tacgtatttg
      721 aaagccttcg tccgactcca gaactatcat ttgcagttcg tcatctcaac tgtttcgcag
      781 gtatcatggt cacagccagc cacaaccctg ctccatttaa cggttacaag gtttacggtg
      841 aagacggtgg acaaatgcct ccacacgatg cggacgcttt gactacttat atccgtgcaa
      901 tcgaaaaccc atttgcagtt gaagttgctg atgtggaaac tgaaaaagct tctggcttga
      961 ttgaagttat cggcgaagct gttgacgtag aataccttaa agaggttaag gacgtaaaca
     1021 tcaacccagc cttgattgaa gaatttggta aagacatgaa gattgtctac acaccacttc
     1081 atggtactgg tgaaatgttg gctcgtcgtg ctcttgccca agcaggattt gactctgttc
     1141 aagttgttga agcgcaagca actgctgacc cagacttctc aactgtaaca tctccaaacc
     1201 cagaaagcca agcagccttt gcccttgctg aagaacttgg tcgtcaagtt ggtgcagatg
     1261 ttcttgtcgc aactgaccca gacgctgacc gtgttggtgt tgaagttctt caaaaagatg
     1321 gtagctacct caacctttca ggtaaccaaa tcggtgctat catggctaaa tacatcttgg
     1381 aagctcacaa aaacgctgga actcttcctg aaaatgccgc tctctgcaaa tctatcgttt
     1441 caactgactt ggtaacgaag attgctgaaa gctacggcgc aactatgttc aacgtcttga
     1501 caggtttcaa atttatcgct gaaaaaatcc aagaattcga agaaaaacac aaccacactt
     1561 acatgatggg atttgaagaa agcttcggtt acttgattaa accatttgta cgtgataaag
     1621 atgccatcca agccgttctt gtcgttgctg aacttgctgc ctactaccgt tctcgtggtt
     1681 tgacacttgc tgacggtatc gaagaaatct ataaagaata tggctactac gcagaaaaaa
     1741 caatctctgt tactctttca ggtgtcgatg gtgctgaaca aatcaaagcg attatggcta
     1801 aattccgcaa caatgctcca aaagaatgga acgcaacagc tatcactgtc gtagaagact
     1861 tcaaggcaca aactgctact gttgctgacg gtactgttac aaacttgaca actcctccaa
     1921 gtgatgtgtt gaaatacaca cttgctgacg gttcatggat tgccgttcgc ccttcaggta
     1981 cagaaccaaa aatcaagttc tacattgcag ttgtaggaga aaccaacgaa gaatcacaag
     2041 ctaagattgc taacatcgaa gcagaaatca atgcatttgt aaaataaaga cctataaaag
     2101 atgagaccaa ccaacctcat cttttttcgt atcaaatcta agcaatttta gaaactttat
     2161 ggcatactag acttatcaat gaaagcgagg taatctcatg gaacaatttt tagataacat
     2221 caaagacctt gaagtcacta cagttgtgcg tgcgcaagaa gctcttgata aaaaagaaac
     2281 tgcaaccttc tttatcggtc gcaaaacttg cccttactgc cgtaaatttg caggtacatt
     2341 gtcaggtgtc gtagctgaaa ccaaagctca catttacttc atcaatagtg aagaaccaag
     2401 ccaactcaat gatttgcaag cattccgctc acgctatgga atcccaactg taccaggttt
     2461 tgttcatatt acagatggac aaatcaacgt ccgttgcgac tcttcaatgt cagcacaaga
     2521 aatcaaagat ttcgcaggat tgtaaaaaat cagctaacaa gtcttaaaat cacaaaaacg
     2581 cataatgtaa ggtgcaaaaa ccttgatatt atgcgtttta ttgttcctaa aacagttaca
     2641 tcagcatttc attacagaac attttaattt tggaagtcaa atcaattttt aacaatgttt
     2701 tagaaatcac agtgggctat tccatattaa attatccaga ttcaatcgct attatcgttt
     2761 tcctagatta caaaaaaact gcgttttgct aatcaaaacg tagtctcagc cactatttta
     2821 tcttactgtc cttcttttac ttctttggtt gctacatctt ctccaaacca tttttggctg
     2881 atttcttgga acttgccgtc cttgtaaaga ctagaaaaag cttcatttat cttcttaacc
     2941 aaggttgtat cttccttacg ggctccaacc gcaaaagctt ctgtttctag tccaactgta
     3001 aagacattat aatcgtttaa aacaccttct gcttctaaat aatagtttgc atagacacgg
     3061 tcaatcaata gaccatcaat tcgatcgttt ttcaaatcaa tcaaggcttc attaaaggtt
     3121 tggtattgat tcgcttcctt attagcgaca atattcttca aaatttctgg atttgcttca
     3181 aagtccgcat aaccagatga accagcttga gctcctaatg tctttccagt catatccttt
     3241 gcagtcgtga taccagatga tttcttcgta accaatacct gctcattctt catatatgag
     3301 ttactgaaag ccaccttttc acggcgttcg tctgtagcgg aatagccatt ccaaatcaga
     3361 tcaatcgttc cttttgtcaa ttcagcttct ttcaaatccc aatcaatcgg ttgccaattt
     3421 accgtgattc cgtatttttc aaaaacagct gtagctaaat caatatcaaa tcctgcataa
     3481 gaaccatctt tctgagcaaa tcccattgga acaaaagtac tatcaaatcc aatagtaata
     3541 gacttgttag actggtactt tgaccaatta tctccactag tttcgctaga atttttccca
     3601 caagctacta agaacaaagc agtcatcaga ctgactaata caagcatcca ttttttcatc
     3661 catttttcct cctatttagg ttctactttc aataatacat ctgcgatatt ttcagcaaac
     3721 tgcaaatcat gggtaaccac aatctgggtc atcccaagtt ccctattttg caagattagc
     3781 ttctccactt ccaaacgtaa ttctggatcc agggcagaag ttggttcatc gtagccaatg
     3841 atttctgggt caatcatcat agcacgcgcc aaagccaccc gctgcttttg cccaccagat
     3901 agtgagaaag gataggcctc tgcgtgtcct cctagtccta actgttccaa gagtccactc
     3961 gccttcttct cagcctcttc ctgcttcatt cccatggtct tcacaggcga taaggtcaaa
     4021 ttgtccagaa ctgataaatg agggaacagt tgaaaatctt ggaatacaaa tcccagtaga
     4081 ttgcgcttct gcaattcatc cagctctaaa ggttgtccat tataaaagat ttgccctgaa
     4141 tcaatggttt caagacctgc aagcatacgt aaaagagttg tcttacctcc accagaaggt
     4201 ccaacgatag ccaggatttg cttttcagga atacttagac tgaaattaga caggatttgt
     4261 ttgtctccaa agactttatt gatatttcgt aattctaaca tggcagcctc ctatctataa
     4321 taactatact tcttctcaac ttttttggaa ataattgtca caatcccaat caaaatcaaa
     4381 taaatggctc ctgccaagaa cataggaact agactagcat cgcggttagc agctgttcga
     4441 ctagccaaga taaggtctga aattccgaga gcatagacca aagaagtatc cttgaccaaa
     4501 ctcataactt cattaaagac actaggaaga acgatcttgg tcacttgggg caagataata
     4561 tagcgcactc tgtcaaaagg gctaaacttc aagaccttgg cggcctcata ctgtcctctt
     4621 ggaatagtgt caattccccc acggaaaatt tctgcaaagt aagctgcata gttgagaaca
     4681 aaggcaataa tagctgcagg aaggcggtct aaacgaatcc caatacttgg gagcacataa
     4741 tagataaaaa tcagttgcaa gagtaagggg gttcctcgca taacccagat gtaaatgtta
     4801 atcagataat ggaggggctt ccaatggact tgcaaggcaa aggcaatcaa aacgcccaag
     4861 ggaatcgaaa atagcaagac cagtgcaaag acctgtacag tcgtgctcgc accattcagt
     4921 aaactcggta atatctcaaa taaataagac atactgcacc tcctaaaagt aattgttcct
     4981 attatagcat gaataaacaa gattaccaag tatttttgta aaaagattct accatataca
     5041 aataaaaacc gtagctatca aaagagataa actacagttc tatatgttta aacattctta
     5101 caatctctca aacagttctt gcatttgcac atcatctgga accagtttta agtaagtgtg
     5161 agcatggact tttgcttctt caaaatgtcc caattcacgc aagagataga tatagtgttc
     5221 cagaaattct ggattgtcct tcaaatctcc tatcaactct tgataatgct cataagcagt
     5281 atccaaatcg tccatttctt gataagaacg agcaatcatc cacttggtca aaagattttc
     5341 tgactcctca ctctgcaagt ctagaatatc ctcataacgc tcctgctcca gataaatagt
     5401 ggctaaacga agcaagattt cttctgtatc ctcagcgtct tcttttgcag taaggagata
     5461 attttctgca ccactagcat catgcaattc ataagaaaat tgtgaagcag ctagcaagag
     5521 gcgagtttca aagggatttt tctctaatcc ttgcttagcg atacgcaggg cttcttgaac
     5581 ttgatgttcc ttatgtaaag cctgactgta cccatactca tagccttcaa agtcaggaga
     5641 aatggtatca agctgcttaa agtagagggt ggctttttga tattcttctt gatcaaaata
     5701 aagactggcc aactcaaaag ctgttaagtc atcgtattct aactccaggg ctttttctaa
     5761 aaactcagta gccgtttcaa atttccctaa ctgagcatag gcaaagccaa ttcgttgata
     5821 ggtggaaatg cccgtttgct cataaatcga gcgattatct aactgggcat aggcttgaat
     5881 agccgcttgg taattttcca actcactatc caactctgcc aaacccaata tcaagagaga
     5941 atcctctgag taggtcaagg cctccaataa tttctcacgt gccacatctg tcaaaccttc
     6001 cagctggtaa aggtctgcct tcagagccaa agacgagaca taccagtcac tgtcagcttg
     6061 gatttcctca agataggtaa aggcttcttc tatttgacca tcctcgctag caattgcagc
     6121 tagattaaga tgaacctctg gaaaatcctc tacaattttc aggtaaattt ccttggcctg
     6181 aggatagaaa ccaatccctt caagataagt tgccaactca tacagaagat cacttgaatc
     6241 attttctaaa gctttggcga aataatgctc agccttagtt aaatcttgct cctccaaagc
     6301 ctgtaacatc tgttgactat tgttcactct tgacttcctc accctctaat tcatatagac
     6361 cactgactac cttataccat tcgaaaatgg ctgaaatgac aaccttagca gaggcataaa
     6421 ccggaatacc aagtaaaact ccccagatac caaacataga tcctgaagtt aacaaaacaa
     6481 agagaacatt aatagggtgg atgtttaatt gacttcccaa aatcaatgga gagacaaaac
     6541 ggccttcaat agtttgttct acgataaaga caatcactac tttcaaaagc atgactggac
     6601 cagcaatcaa acccaatact agagcaggaa gcatggctag aaagctacca agataaggga
     6661 ccagatttaa aataccagca gtaaccccca gcgtaaccgc atagcgtaga ccaataatct
     6721 tgaagaagat gataaacatt actgctacaa taatagccac tgtcacttgc cctcgaacat
     6781 agttggacaa ctgttgattc acatctgata aaacttgtcc aacaggttcc ttcaattttc
     6841 ttggaatgaa ttgggtcaaa tagttacgca agcctttccc atcacgcaag agataaaaga
     6901 gcatgaaagg aacgataatc aaggcaacaa tcacttgaga agctccgcta ataaaggcac
     6961 tcacccagtt gactgcctga gatgaaacct tacttgccaa aactgtagcc tggctagaaa
     7021 aattggtcaa aacttgctct aattgaggtc tgaaatcatc tggcaggtgc tgggctacca
     7081 atccattaac aatcctatct atatcttcta agtaaacagg aacgtttctt gcaaaggtca
     7141 aaacctgacg ttgcagattt ggaatggcga ctgccaagcc ccaaatgata aagagagcga
     7201 tgataacaaa gacaatagtg atagctataa cacgattaac cttatgcttc tccatccaat
     7261 caacaatagg attcaacaaa taatataaca aaccagacaa aatgactggc aacatcacaa
     7321 ctgctaaaaa gtctaaaaca ggtgaaaata gaaaactaat cttacttaaa ataaaaagat
     7381 tcagtcccaa taataaggtt actaaaaata ccgtaattgc cttgttatct aaaaaccact
     7441 tgaaaaacca agataggcta aaatgtttct ctttttgctc cataaatact tcctttctat
     7501 tgttctctca ttataccatt tttgaaacaa aataactctt ttttaaagtg cttacatcta
     7561 attggatatc tttaatttac ttgtttttgt gatataatag tcttatcttt acacagaggt
     7621 atagtaatga aagaaactgt ttattttgga acttatacac gtcgtacttc tcaagggatt
     7681 tacaaggcag actttgatac agaaactggt cagctttcaa atctagaact ttttgcagct
     7741 gagccaagtc caacctacct tgcctttgac cagcaccaac atttatacac tgttggtagc
     7801 caagacgata aggggggaat tgcagcctat caaactgacg ggactgtgtt aaatcatgtt
     7861 gttgaagaag gagctcccca ctgttatgtt gctgtcgatg aaaagcgtga tttggtttac
     7921 gcagccaact atcacaaggg acaagtcctt gtttataaac gccaggaaga tggtagtctt
     7981 ctacttagtg atatggatca acacagtggc caaggtccac atgaaaatca agcttccccc
     8041 catgttcact atacagattt aacacctgac cactatctag tgacctgcga cttgggaact
     8101 gaccaagtca tcacctatga cctcgatcaa gaaggaaaat tatctaagct ctatacctat
     8161 cacagcaagc caggagcagg ctcacgccat atcattttcc ataaccacta taaaatcgct
     8221 tatctcattt gtgaactcaa tagtactatc gaagttttaa tctacgatgg cgttggcgaa
     8281 tttgaacgta tgcaggtcat ttcaacttta ccggaagctt acgaaggctt taatggaacc
     8341 gctgctattc atctctctaa agacggtaaa tacctctacg cttctaaccg tggccatgat
     8401 tctatcgcag tatataccat ccttgcggac ggtagcttag agttattaga aatcgttcca
     8461 acgcatggtc agactccacg tgattttgat ttgacacccg accaaaaatt tctcattgtt
     8521 gtccatcaag actctgacaa tgcaactgtc tttaaacgta attgtgacaa tggtcgtcta
     8581 gcagaactct ccaacgactt ccatgttccc gaagcagtct gcatccgttt tgctccttaa
     8641 aacaccataa aaaatgaact tggtattttc aagttcattt tttcttatag tctatttccg
     8701 acattaatac ggttaatagc acgttgcaaa gcaatcttag cacgacgttc ttggtcaatc
     8761 aaatgtttgt cttgtgcttc ttcaattgca cgttctgcac gaagtttggc acgttctgca
     8821 cgactgatat cgatatcacg agcacgttct gcagagtcag cgacgattgt gatcatatca
     8881 ttggcaattt caataacacc accgtttact gcaatccagt tcacttgatc tttatcatcg
     8941 atacgtttta cctttacttc atcaactgct aaaaccgcaa tcatattttc atgtcgtggc
     9001 aagatcccca tctcaccatc cagagttcga accgatacat agctggcatg gtgatcatag
     9061 acgagaccat ctggtgtcac gatctggaca gttaactgag ccatagttca cctcttaaaa
     9121 tcccattttt tcagcttttg caatcacatc ttcgatagaa cctacaccac ggaaggcatc
     9181 ttctggcaag tggtcgtatt taccatcaag gatttcctta aagccacgta cagtttcagc
     9241 aactggaaca taagaacctg gctgaccagt aaattgttcc gcaacattga agttttgtga
     9301 caagaagaac tggatacgac gggcgcgagc aaccaaggtc ttttcttcat cagaaagttc
     9361 atccatacca aggatagcaa tgatatcttg caattcatgg taacgttgaa ggacacgttt
     9421 tacttcagca gcaactgcat agtgctcttc tccaacgatt tcaggtgcca aggcacgtga
     9481 gcttgaagca agtgggtcaa cggctgggta gatacccaat tgtaccaact tacgttccaa
     9541 gtttgttgtt gaatccaagt gagcgaaggc tgttgctggc gctgggtcag tatagtcatc
     9601 cgctggcaca tagatagcct ggatagaggt tacagaaccc ttcttggttg atgtgatacg
     9661 ttcttgcaat tgacccattt ccgtagcaag tgttggttgg taaccaacgg ctgatggcat
     9721 acgacccaaa agggcagata cttctgaacc agcctgagtg aaacggaaga tattatcgat
     9781 aaagagaagc acgtcttggc cttccacatc acggaagtat tcagcgattg tcaaaccagt
     9841 aagggcaaca cgcatacgtg ctcctggtgg ctcattcatc tgaccaaaga ccatggctgt
     9901 tttctcgata acgcctgatt ctttcatttc ccagtaaagg tcattcccct cacgagtacg
     9961 ttccccaaca ccagcaaata ctgaaatacc accgtgctct tgggcaatgt tgtgaatcaa
    10021 ttcttggatt aagacagttt taccaactcc ggcaccaccg aaaagtccaa ctttaccacc
    10081 tttaaggtaa ggggcaagaa ggtcaataac cttgatccct gtttcaagga tttcagaaga
    10141 ggtagacaac tcatcaaaag ttggagcttt tttatgaatt ggctgacgct ctgcgtcttc
    10201 tgtaaaagga gcttccaagt caatggtatc tcccaaaacg ttgaagacac gtcccaaagt
    10261 ttctttacct actggtacag agattggacg acctgtgtcc aatacttcca ttccacgagt
    10321 caacccatct gttgattcca tggcgatagt acgaaccata ccatctccta actccaaggc
    10381 tacttcaagg acgatttttg tttttctttc gtcatttttg tagacgacaa gtgcattgtt
    10441 aatctcagga agtttttccc ctgctgcaaa caaaacgtct acaacgggac cgataacctg
    10501 agcaatttta cctgaactca tctccttctc ctattctata taagatactg tcggttccta
    10561 gctcaactag gtcctaagtt cattttcata cgagctggac tagagcctat tctaaggcac
    10621 tagcacctgc tacgatttct gtaatttctt gtgtaatcgc cgcctgtctg gcacggttat
    10681 actgaattgt caaatcattg atgactttct tagcattatc tgtcgctgtt tgcatggctg
    10741 tcatacccgc agcattctca gctgtcttgg catctataat ggcaccgtaa atcatacttt
    10801 ctgcaaactg aggcaacaac tgctccagaa tttcttctcg gctggtttcc aattcaaaag
    10861 tcaagctgta ctcttcatcc gcttcatttg gatccaagtc aacaatcgga agcatttgtt
    10921 ccacacgcat ttgactggtt agcgtattga catggtggtt gtagcaaaca taaagctcat
    10981 caaagagttc attttggtac atttcaacag tttttgaaat aatcttacga acttgatcaa
    11041 agctaggttg gtctgccaag ccacgtaatt cataaagtgg ttgaataccg cgagccttaa
    11101 agaaatcagc tcccatccca ccgatacaga tcatttcaaa acctttaccg tctgggtggt
    11161 attcttcttt caactccata acagctttca aaatagagga attataacct ccaaccaaac
    11221 cgcggtctga agtgataacg atatagcctg tcttcttcac agaacggcta atcaacatcg
    11281 gattagttga agcaccagct ccattaccat gaaggatatc tgtcaaaagt ttacgcactt
    11341 tctgagcgta aacttggaag ttgcgagcag cttcttcaga gcgacctagc ttagcagccg
    11401 ataccatttg catggcatta gtgatttgac tcgtattttt tgttgaggcg atttttgttt
    11461 taatatcatt tagagatact gccatctgac acctctattc ttattggaag ctagattgat
    11521 tgagaaactc tgtaatcgca gcatccaaga ctgcttcttc tggcaagtct tttgtatcac
    11581 gaatggtttc caaaatctct ggatgttgag catcaaagaa ggcatggaac tcttcctcga
    11641 aacgaacaat atcatctact ggaacagtat ccaagaaacc atgtatcaaa gcataaagaa
    11701 tggttacttg tttctcaaca ggtaatggtt tgtgaacagg ttgtttcaag acctcaacgg
    11761 tacgacgtcc acggttcaac ttagcctgtg ttgctgcgtc caagtcagaa ccaaacttag
    11821 taaaggcttc caactcacgg tatgaagcaa ggtcgatacg aagtgtacca gcaaccttct
    11881 tcatggcttt gatttgtgca gaaccaccta cacgagatac agatgaaccc gcatcgatgg
    11941 ctggacgaat acctgcattg aagaggccat cgccaaggaa gatttgtcca tcagtgatag
    12001 aaatcacgtt ggttgcgata taggctgaga tatctcctgc ttgtgtctcg ataaatggta
    12061 gggctgtaat tgatccacca ccaagttcat cagaaacttt agctgagcgc tcaagcaaac
    12121 ggctgtggag atagaaaaca tcccctggga aggcttcacg acctggagga cgacgaagca
    12181 agagcgacag ttcacgataa gctaccgctt gttttgatag atcatcgtat acaatcaaaa
    12241 catgcttacc ttgatacata aattcttccg ccatagcgac cccagtataa ggagctagga
    12301 agagcaatgg agatggttgt gaagcagagg ctgtcacaac gattgtgtag tccaaggcac
    12361 cgtactgacg aagtgtttct acttgcgtac gaactgttga ttctttttgt ccaatcgcga
    12421 cgtagataca gatcatatct tgatcttttt ggttcaagat tgtatcaatc gcaatggttg
    12481 ttttccctgt ctgacggtca ccgataatca actcacgttg accacgacca atcggtacaa
    12541 gggcgtcaat agctttcaaa ccagtttgca atggttctga aacagactta cgttgcataa
    12601 caccaggagc tggtgcttct actggacgag ttttatcagt gtggatttct ccaagaccgt
    12661 caactggacg accaagcgga tccacaacac gaccaatcag actttcacct acagggactt
    12721 ccatgatttt ccctgtacgg cggattgtat cgccttcacg gatatctgta aagtcaccta
    12781 ggatgataat accaacgtct gttgactcca agttttgagc cataccataa gagccgtttt
    12841 caaaattcaa caactctcca ctcatgacat tttcaaggcc gtgagcacgc gcgataccgt
    12901 ccccgatata ggttacaaca cctgtttcag tcacatcaaa attgggtttg aaattttcaa
    12961 tttgttgctt aattaaagcg ctgatttctt gtgcgttaat tgccaaaaga acaccacttt
    13021 ctatttcaaa ttttctttaa caactttaag ttgttgttta atactcacat caattgtctt
    13081 gtgattggca aaaatgacaa aaccaccaat gagactttca tcgatttgtt cttttacact
    13141 ccttactttc agagacattt ttttctcaat caaagggagc aaacgagtct tctgttcatc
    13201 agttagagga tgagcagacg taatcgtcac ttcaaatcga tttgtttctt tttcaagtcg
    13261 gttcaagcaa tctacaagca catcataaaa aagatttgct ctgtgattgt aggccagaac
    13321 ctggataaag ttttgtaata aaggcgacac agaatcttgg aaaaaagcaa ttgttttttc
    13381 cttatccgac tcgtctactg ccacttgttt taaaaaagaa ggcagacctg ttttttcaac
    13441 aacttgcttg atttgagtca agtctgaaaa gatacggtct tcttctcctt tttcaagtac
    13501 caattggaca aaaggcatgc tgtatttttc aattaccttt actgttttct tgtccattaa
    13561 gcttctccta gctgatcgat atactgatca atgagtgctt tatgggcatg actgtcaagg
    13621 ttttgtgaga tgattttacc agctaagctg atggtcagat ctgcgacctc acccttaaca
    13681 ctctgtaaag cttctacttt attttgagca atttcttgat tggctttttc ttttaagtgt
    13741 cctgcttcta gtttagcatc tgctaagata ttagccttac tttgctcagc tgtttccttt
    13801 gcattttcaa tgattgtctt agcttcttta cggctaccag ccaattcatc ttcgcgtttt
    13861 tgagccaata cttctgcttt ttgacgggct tcttcagctc tgtcaatatc tgaagcaatt
    13921 ttttcagctc tttcttcgaa aatgcctgta atattagacc atgcaaattt tttaatcaag
    13981 actagcaaaa gaataaaaga gccagtgatt aaaataaaat taccaattaa ttcacctact
    14041 gttacgtgca tcagttactc ctttctattc ctcttcaata ttaatcttat tccctaaata
    14101 cacagatgtc aaaagagtaa aaacataagc ttggatgcag gaaataaaga cagaaaatgc
    14161 agtccaagcc aaattagctc caaaggctac tggataccaa taaatagctt ggtgggaaag
    14221 aagaagtaac aaactcgtca tgacctctcc tgcaaagata ttcccaaaaa tccgcaaagc
    14281 caaagataag aagtttgtaa attcttccaa gatattcatc ggtatgacaa aaacaggact
    14341 cataaaagat tttatacttt ttttaaatcc acgacgacga acgctttcta tatgtgtcaa
    14401 caaaatgaca agaaaagata aggttaagtc atactgtaaa ttagcggttg gcgaactcca
    14461 ccagttagtc ccatcgatcg tttgaagctt tgtcattaag ccaaggttat tggcaatcac
    14521 catgaaaagg aataaacaaa ggaaaaagag tgaataatct ttcatgtagc gcgaaccaat
    14581 gttaggttct gtaaatccaa taacaaagtc atagacatac tcaagtacat tttgctttcc
    14641 tttgggtttc aaggtcatat tgcggcttgc ccaataaata aagacaaaaa taactcccac
    14701 aatcaacaaa gtcatggcta acatagtcag attgaagata acaggaccaa tagagatgat
    14761 tggattaata ctttcttcca tctaaaatcc tcccttttct tctcgttcca tgtatttatt
    14821 tgataataaa tgagaagaca agagttacaa agaaagttcc ttcaataaag gcaacaccta
    14881 aaaacatcaa actacgaaac tcagaaagca tatctggttg gcgtgctact gatttaaaca
    14941 gtccattcat caataaacct tcaccgacag atacgcccat acaggcaata cataagccta
    15001 aaaatgttaa attcataaca aattctcctt taaaataaaa tttacttact tagtgtagtc
    15061 ctaaaattta aatttgtcaa cttttaaccg ctattggaag cgtttaataa aaattatttc
    15121 cattttgcta aatttcaagt tcaagttagc catcca
//
LOCUS       DAVBBS010000050        15044 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVBBS010000050 DAVBBS010000000
VERSION     DAVBBS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15044
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.050"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            189..527
                     /locus_tag="VQU32_001604"
     CDS             189..527
                     /locus_tag="VQU32_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET2473032.1"
                     /translation="MTLTIHVYYKGDGDCAIKFAREMLESGLVAEIRCQKGNLKYEYF
                     LPIEKEGIILLIDQWINQEALDEHHQSNTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
     gene            717..2153
                     /locus_tag="VQU32_001605"
     CDS             717..2153
                     /locus_tag="VQU32_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET2473033.1"
                     /translation="MTTFKDGFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITLGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPVTCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTDQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKKVILSNGESVE"
     gene            2205..2444
                     /locus_tag="VQU32_001606"
     CDS             2205..2444
                     /locus_tag="VQU32_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HET2473034.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            2574..2888
                     /locus_tag="VQU32_001607"
     CDS             2574..2888
                     /locus_tag="VQU32_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HET2473035.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            3006..4985
                     /locus_tag="VQU32_001608"
     CDS             3006..4985
                     /locus_tag="VQU32_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET2473036.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFAQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKNISEEVLKKSLFKTFQNLGLDDMYNFSSDFQLIEGLIT
                     HLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVS
                     LHFLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQE
                     KLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINK
                     FENNSFRQYFSKENFLICTESDKVNLLEKMVESLSVGESNEFEQSLLYGIKQREELSS
                     VVFSEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSYVQLVFLLSPSIYGNEGLAT
                     VTKKIVSLTENDELQNQLISCNNFEDFISIFEKIK"
     gene            4995..5309
                     /locus_tag="VQU32_001609"
     CDS             4995..5309
                     /locus_tag="VQU32_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HET2473037.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            5340..5846
                     /locus_tag="VQU32_001610"
     CDS             5340..5846
                     /locus_tag="VQU32_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473038.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFINPSPIIIFPIMLIVFSILATVEQFKLYGNRKEKLGITLMFVRIQILISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     LEKNSLSL"
     gene            5926..7272
                     /gene="celB"
                     /locus_tag="VQU32_001611"
     CDS             5926..7272
                     /gene="celB"
                     /locus_tag="VQU32_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HET2473039.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            7652..7828
                     /locus_tag="VQU32_001612"
     CDS             7652..7828
                     /locus_tag="VQU32_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473040.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            8238..10451
                     /locus_tag="VQU32_001613"
     CDS             8238..10451
                     /locus_tag="VQU32_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HET2473041.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHFFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(10673..11149)
                     /locus_tag="VQU32_001614"
     CDS             complement(10673..11149)
                     /locus_tag="VQU32_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HET2473042.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            11372..14572
                     /locus_tag="VQU32_001615"
     CDS             11372..14572
                     /locus_tag="VQU32_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HET2473043.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSADASTRVIEAKDGA
                     ITISSHEKLRAAVHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESDKDKRLWNSA
                     TTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQPSEDYQTD
                     KQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKEV
                     KKIPLKILASVKDAYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSIS
                     SQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSMEW
                     MHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDPE
                     HFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGFY
                     QDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAPL
                     LVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQS
                     DSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGFG
                     LSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTET
                     DAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFLG
                     SNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDSK
                     KNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYML
                     IPNVGRATFNQMIKELERSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVLK
                     RGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKNH
                     SDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            14952..15044
                     /locus_tag="VQU32_001616"
                     /pseudo
     CDS             14952..15044
                     /locus_tag="VQU32_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005576373.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         5010 a         2193 c         3089 g         4752 t
ORIGIN      
        1 gaggctcttt tgtcttgttc ttgtttcaat tgactataaa tcgctataag aaaaaatcat
       61 tttattggta taaagaagtg attgaaagta atggcgaaac gttagataat tagttcataa
      121 aaaacaagtt tagatcctca tctaaacttg tttttttaaa attgaaggat gattttggag
      181 gatatgagat gacattgact attcatgtat attataaagg cgatggagat tgtgcaataa
      241 agtttgcaag agaaatgctt gaatctgggc ttgtcgcaga aattagatgt caaaaaggta
      301 atttgaaata cgaatatttt ttaccaattg aaaaagaggg aattattctc cttattgatc
      361 agtggataaa tcaagaagct ttagatgaac atcatcaatc aaatacaatg cagaaaatct
      421 tagatttaag aaaaaaatat cacttgcaga tgcaagttga acgttatata gaagatgatt
      481 ctgggatgcc agaaagcgat aagtcgttta tagacacagg gaattagaat tattgtgaga
      541 gtttttgtag tttgaaagta ttgaaaatcg taaagatatc attacgagaa tacaagaaga
      601 tattgttcta tttccattaa aaattacttc tttccttaat aataaggaaa aagaatacgt
      661 tttcatcttt tggtttgagg tttataataa gaatatcaaa gtcaggagga aaaaagatga
      721 ctacttttaa agatggattt ttatggggtg gtgctgttgc tgctcatcaa cttgaaggcg
      781 gatggcaaga aggtggcaaa ggaattagtg ttgctgatgt tatgactgct ggtcgccatg
      841 gagtagctcg tgaaattact ttgggagttt tagagggtaa atattatcca aatcatgagg
      901 cgatagattt ttatcatcgt tataaagaag acatagcact ttttgctgaa atgggattca
      961 agtgcttccg tacctctatt gcatggacac gtatctttcc aaaaggtgat gagttagagc
     1021 cgaatgaaga aggattacag ttttatgata atctttttga tgaatgctta aagaatggta
     1081 ttgaacctgt catcactcta tctcattttg aaatgcctta tcacttagtg accgaatatg
     1141 gtggttggaa aaataggaaa ttgattgatt tctttgctcg ttttgcagaa gtcgtattta
     1201 aacgttacaa agataaggtt aaatattgga tgactttcaa tgaaatcaat aatcaagcga
     1261 attatcagga agattttgca ccatttacta actcaggtat tgtatatgag gaaggtgata
     1321 atagagaagc aattatgtat caagcagcac attacgaatt agttgcttct gcacgagctg
     1381 taaaaattgg tcatgagatt aatccagatt ttcaaatagg ttgtatgatt gcgatgtgtc
     1441 caatttatcc agttacttgc aatcctaagg atatcttaat ggcaatgaaa gctatgcaga
     1501 agcgttatta ttttgctgat gtgcatgttt taggtaaata tcctgagcat attttcaagt
     1561 attgggaacg aaaaggtatt tcagttgatt ttactgacca ggataaagaa gatttacttg
     1621 gtgggactgt agattacatt ggtttcagtt actatatgtc ctttgctata gactctcatc
     1681 gtgaaaataa tccttatttt gattatcttg aaacagaaga tttagtgaaa aataattatg
     1741 ttaaggcttc tgaatgggag tggcaaattg atccagaagg tttacgttat gcgttaaatt
     1801 ggtttacaga ccactatcac ttaccactct ttattgttga aaatggtttt ggagctatag
     1861 atcaagttgc agcagatggt atggtacatg atgattatag aattgaatat ctaggtgccc
     1921 atattcgtga aatgaaaaag gctgtagttg aagatggtgt tgatttaatg ggttatactc
     1981 catggggatg tattgatttg gtttcagctg gtaccggtga aatgcggaaa cgttatggct
     2041 ttatttatgt agataaagat gacaatggga agggaagtta taatcgttcc ccgaaaaaat
     2101 cttttggctg gtataagaaa gttattttat ctaacggtga atcagtagaa tagtataaac
     2161 aaactgagat tattctcagt ttgtttagca tattgaggag acatatgatt ggaagtattg
     2221 cagcaatttt aacaacattt gcatttttac cacaagtttt tcgtgtagtt aagacaaagg
     2281 acacaggatc tattgctcta ggtatgtatg ttatgcaagt tattggtatt gcattgtggc
     2341 ttgctcatgg tattcgtata ggagacttgc ctttgatttt agcaaatagt gtttcatttc
     2401 tactctcagg aataatttta ttttataaat tgaagtataa ataatttttt tccatatcat
     2461 ttaggaaaat gagggaattg tcattgaaaa cgctttattt tataataaaa atagataaca
     2521 aataagccaa cttagcacag ctttgattgg atttaagaaa aggagaaaat attatggcaa
     2581 aagctttaat tatttgtgca gcaggaatgt cttcatcact tatggcacag aaaacaacag
     2641 agtttctaaa aggaaaaggg aaagatattg tagttgatgc tattagtgct actgaaggag
     2701 ggaaagctat cgcagcagca gcttttgatt tgtatcttgt cagtcctcaa acaaaaatgt
     2761 actttaagca atttgaagag gctggttcta aagcaggaaa accagttgta caaatacctc
     2821 ctcaagccta tattcctatt ccaatgggaa ttgaaaaaat ggctaatttg attcttgaga
     2881 atatttgatg aacacatgaa atgtcaataa atatttaagt taagataaat tgttttgagt
     2941 ttgatataaa ttgatttaag tgtataatat gcttcaatat atgaaaacat acgttttagg
     3001 agggaatgaa tgtgttaaac attaaagaga aagatatcct tcaattttta ataaaaaata
     3061 aggaacgttt tgttacaagc aaagaactgg cagaatattt atcctgttca gatagaactg
     3121 tccgtaatgt actgaaatta attgagaaga caatgattat tcaaggagtc cggttgattt
     3181 caaaacaagg tcaagggtat caaatatttt ttgagaatca aggcgcttac caggagttta
     3241 gacaaactta tgaactggaa gaagactata cgaaaactgc ggtttcaaaa ggtgatgatc
     3301 gtttagtatt tatcttaaat aaattattat ttgagcaagt tccagttctt tttgatgatt
     3361 tagctgatga actttacgtt agtcgttcaa cattatctca tgatttcaga aagatacgag
     3421 tgatgttatc agagtataat ttgtccattg agagtagggc gaataaagga gtttatgtct
     3481 caggcgagga acgtgataaa cgacgtttta tcataaatta tttcctagag aatcaatttt
     3541 ttaaaacaat acattgctat gtaaaattta acttttttgc tcaaactgtt ccattagaag
     3601 aatttgctag aattgttttg gatgaatgcc aagaggccaa cctaaaacta tctgattttg
     3661 tactacagaa tttagtagtt cacatagcat tgtctgtgat tcgtttaaaa tccggatttg
     3721 aaataaaaaa tatagactgt cagatgactg atgatgcaat agagagaaag gttgctcaaa
     3781 gaattctttc taaagtaaga gaagtaacaa atcaggaatt tccagttcag gaaatagact
     3841 acattacttt gcatttgtta gctaagagtc aacaatgtca aaaaaatcag aagaatattt
     3901 ctgaagaagt tttaaaaaaa tctttattta aaacatttca gaatttagga ttagatgata
     3961 tgtataactt ttcttcagat ttccaactaa ttgaaggatt gataactcat ctaatgacac
     4021 tacaagtacg tttagaaagc cggattacct taaataaccc tttggtagat gaaattaaac
     4081 agaattatag tgatatattc tttatgacta gagaaattct tgcaaatatg gacaagttct
     4141 tggagtggac aatatcagat gatgaagtag catatgtaag tcttcacttt ttagctgcta
     4201 tggaacgtag caaagagagt actaagttta atattcttgc tatttgtgca acaggatttg
     4261 gtgcagccca aatgttaaga aatcgtttgg aaacggagtt tgggaagcga gtagaagttg
     4321 ttgatgtaat aggctattat gaattgaatc aagaaaaact taaaggaata gattttatag
     4381 tgtcagctgt tgatttatca aatctatatt ttcaaattcc agtttttaaa gttagtgtgt
     4441 ttcttaagag tgatgagatg gaaatgatta gaaaagctat ggatcagatg caagtttcta
     4501 gttatgtaca atcatcaaag attaataaat ttgaaaacaa cagctttaga cagtatttta
     4561 gtaaagaaaa ttttttaata tgtactgaat cagacaaggt aaatttactt gaaaagatgg
     4621 ttgagagttt atctgttggt gaatcaaatg agtttgaaca atctttactt tatggaatta
     4681 aacagcgcga agaattaagt tcagttgttt tttctgaaaa aattgcagtt ccgcatccta
     4741 ttcagccctt tggtacagag ggaaaggttt cggttgcaat ctgtaaagat tcgttactct
     4801 gggataatca aagttcatat gtccagctag tatttcttct atctccatca atatatggga
     4861 acgaaggttt ggcaactgtg actaaaaaga tagtctctct gacagagaat gatgagttgc
     4921 aaaatcagct aatatcttgc aataactttg aagattttat tagcatattt gagaagataa
     4981 aataggaggt tcatatgaat caagaggaac tgcaggttgc agcatttgaa attatcctgc
     5041 attcgggaaa tgcaaggtca gaaatacatg aagcatttgc aaaaatgaga gaaggtagtt
     5101 ttgatgatgc agaaagtaaa ttaaatcaat caaatgagat aattcttgaa gcgcatcatg
     5161 cacaaaccaa actcttgcaa gaatacgcaa gtggagtgga aattaaaatt gaaattatca
     5221 tggtacatgc tcaagatcat ttaatgacca ctatgacatt attagaagta gcaaaggaaa
     5281 tgttagctct ttataagaaa gttaactaaa tatgtgttgt tatataagga ggaaatgata
     5341 tggataaaaa agaacgacaa aagattgaac aacaacgtcg agagatggct ctaactaata
     5401 ctttttttaa tcgatatctt cttctacgtt attcaatagc actcttcttt tttgggaata
     5461 tttattggct tttgaatcag tttattaatc cctcgcctat tattattttc cccattatgc
     5521 tcatagtatt ttctatttta gcaactgttg aacaatttaa actctatgga aataggaaag
     5581 aaaagttggg aataacacta atgtttgtta gaatacaaat acttatttca ataggattac
     5641 ttgttttaac ttggacaagt tggtttaaga atctttttcc gatttttgaa aataaccaag
     5701 tagcacgttt atttgttttt gtagttcttc tattaggttt agttctgagt ttgttagaca
     5761 ttagaagaat taaaaaaatt tataaacgaa cagataaggc ttatcaacaa tttgtccaat
     5821 tggaaaagaa ctcacttagt ttataatcct gatctagtat aatttgtatt tgaaactagt
     5881 ctttagaaag taaaaattat tatgttttat taaggaggtc tatttatgga tcaacaaaac
     5941 gggttgtttg gttttcttga aaaccatgtt atgggaccaa tgggcaaact tgctcagttt
     6001 aaagtagtgc gtgctatcac ggctgcaggt atggctgctg taccatttac tattgtagga
     6061 tcaatgtttt tggtattcag tattttgcca caagctttct cattttggcc aattgtggca
     6121 gatattttct ctgcttcatt tgataaattc acatcacttt acatggttgc aaactatgcg
     6181 actatgggtt ctctatctct ttatttcgtt ctatcacttg catatgaatt gacaaaaatt
     6241 tatgcagagg aagaagaact caatatgaat cctcttaatg gtgccttgct cgccttgatg
     6301 gcttttgtca tgacagtacc gcaaatcatt tttgatggtg gaatgatgaa gactgtgaca
     6361 agtctaaaag aaggtgcagt aattgcagat ggatgggcaa tgggaaatgg agtcgcacgt
     6421 tttgggacaa cagggatttt taccgcaatc attatggcaa ttgtgactgt tcttatttat
     6481 cgtatgtgtg ttaaacataa ttgggttatt aaaatgcctg aagctgttcc agaaggagtt
     6541 tctcgtggat ttaccgcttt ggttccggga tttgttgttg catttgttgt tatctttatc
     6601 aacggtcttc ttgtagcaat gggaacagat atttttaaag tcattgcaat tccatttggt
     6661 tttgtatcca atctgactaa ttcgtggatt ggtttaatga ttatttatct attgactcaa
     6721 ctactttgga ttgtaggtat ccacggtgcg aacattgttt ttgcatttgt tagtccaatt
     6781 gctcttgcta acatggctga aaatgctgct ggcgggcact tcgctgttgc aggtgaattt
     6841 tctaatatgt ttgtaattgc aggtggttct ggtgcaactt taggactatg tttatatatt
     6901 gcttttgcct ctaaatctga acagcttaaa gcaataggac gagcatctgt agttccagcc
     6961 ttatttaata ttaatgaacc attaattttt ggattaccta ttatctataa tccagccttg
     7021 gctataccat ttattttagc accaatggtt actgctacta tttattacgt agcgaattct
     7081 ctaaacttta ttaagccaat tatcgcacag gttccatggc caactccagt agggattgga
     7141 gctttcttag ggacagcaga tcttcgagct gtattagttg ctctagtatg tgcatttgca
     7201 gcattcttag tctatcttcc attcatccgt gtatatgatc aaaaattggt gaaagaagag
     7261 caaggtatct aataagtaaa aaagtatact cgatgataga atccatatag tttaaataat
     7321 gagtgttaat attagcagtg aagaggtctt tttaagatat tcttctctgc tttttcatat
     7381 tttttcgcta tgtagggtat gaagcaaaat agaaagtagt tcgaatagtt gataaacagg
     7441 aaactgctgt tatttaaaaa aaacaataaa gtaaagtatg ttatcgtaga atacgataca
     7501 atagtaaaaa agcaaaaaga atgcgtttta tatttgggtt tgaggtttat aaaataggat
     7561 aagcgtgctg aaaacttaga tatgacaaac aaaataaaat ttttaaaagc tacatagaaa
     7621 catggtataa tgtatctaaa ggaggtagca tatgtcctta gatatagata aagaaaagat
     7681 gacaatcatg ggaatagctt ttgaaaacag atccgttttt aagagtgttt ggtatgcttt
     7741 aagtacaaat atgattgaag gatggcgacc tacggttagt gatgttgaaa aattacgaga
     7801 tgaagctctt gctctgggga tgacttaatg aagcgcaact gttatgagtc ttatgactat
     7861 gttgatccgg ataatttata taccgatccg ggttcttcag ttctaagaaa taagcaggaa
     7921 ggaaaaaagc acgcgagctt gaatatcgta tgattgctag taagagtttg aaattattta
     7981 tcaatcctat cttagttaat agtttaaaag tagtttgttt cacaactgca taagcttaga
     8041 aaagttgaaa agtgataact ctttaataaa ctcttggttt gttaaaagat attttgtcca
     8101 ctacattttt tcaattgact attatttgtc atagatataa cagatagaac agatagaaca
     8161 ttgcattcag aataatgaaa aacgcatcaa aaatggtgcg ttctgttttt atgctaccat
     8221 aaaaggagga aaacaaattg aaaattttta agggagagtt ttatcgaatc tctgtattaa
     8281 cagacaagct agtaaggtta gaatactctc aaactggaag ttttgaggat agaacgacac
     8341 aacttatcta taatagagat tttggccaag tttcgttaga ttatatcgag acatcaaacg
     8401 tactagatat tatgacggac tattttcatc tgcactttaa taaaggagaa tttaacgccg
     8461 aaaatttatt tatagaatta aaaggaaatt ttgccgtata tggtagtcgc tggtattttg
     8521 gtgaatctat tgaaacgtta aaaggaacag ctcggactct ggataaggca gatggagcaa
     8581 tctcgttaga agatggaatt attagccgaa atggtatagc cttattggat gattctcaag
     8641 gatttatttg ggatgaacaa tctggttata ttgagagaga aaatcaaatt gacctgtatt
     8701 tctttgccta tgggcatgat tatagaggag caatcagaga cttttaccat ttgactggtt
     8761 caacaccctt gttgccaaga tatgctttag gcaattggtg gagtagatat tggccttata
     8821 cgtcggatga atacttggat ttaatagaca gatttgaaac agagaaaatt ccattatcta
     8881 tcggtgtgtt agatatggat tggcatataa ctgacattcc agctcgtttt ggaagtggct
     8941 ggacaggata tagttggaat agaaacttaa taccaaatcc agaacagtta ttgcaacaac
     9001 ttcatgatag aaagttaaaa ctctccttaa atgtccatcc tgctgatggg atacgggctt
     9061 atgaagaagc ttatcctcaa atcgcaaaac ggttggggtt aaatgtagaa ctagaagaac
     9121 ctgctatttt tgattttttt aatccctctt ttagggaagc ctactttaaa gatgttcatt
     9181 atgagctaga aaagcaggga gtagattttt ggtggattga ctggcaacaa gggacacaag
     9241 gtatgctaga tccactttgg cttttaaacc attatcacta tcaggatagt tgtaaaaatg
     9301 cagaaggtgg tttgatttta tcaagatatg caggtcctgg tagtcaccgc taccctgttg
     9361 gtttttcagg ggatactatt attagttgga attccttaag gtttcaaccc tattttacag
     9421 cgacagcatc taatatcggt tatagttggt ggagtcatga tatcggtgga catatgttgg
     9481 gggattatga cgaagagcta caaactagat ggctacagtt tggtgttttt agtccgatta
     9541 ctcgattaca tagttctaga agtcctttta atagtaaaga accttggttc ttttcagaaa
     9601 caacatctaa gattatgaag aaataccttc gtttgagaca tcagatgatt ccctatctat
     9661 ataccatgaa tgtaaagaca catgaggaag gtgccccatt aatcagtcca atctattatt
     9721 tctatccaga gaatgatgag agctataatg ttccaaacca atactttttt ggaacagaac
     9781 tgatggtggc tcccattgta gaaaagatgg atttgacatt ccaatctgca aaagtagatg
     9841 tatggttccc tgaaggtgaa tggtatgact tcttttcaga gaaaaaatac acaggtggtg
     9901 tgaagttaag tgtttatagg gacatctcga ctacgcctgt gtttgcaaaa agtggtgcaa
     9961 tcattccctt ggttggttct gagataggta tgggtgttga tttacctgag gttgtagatt
    10021 ggtatgtatt cccagggaaa caacattttt ttgaaatgct tgaagatcaa aatggtcaaa
    10081 gatataaaac aagattatca atcgattggg aaatgggaat ggtagagtta gcattacaag
    10141 gagattctag tatcgttcca agcaatagaa aacatagaat tcattttaaa ggaacgaatg
    10201 tgtctataat tgaattgcca aataagaatg atacagctaa atttgaatgg aaagataata
    10261 aaaggacatc tttaaatgac gaagttttta gactactaaa gacagcttct cttccatatg
    10321 aattaaaaga tagattgtta aatcaattca tcaatgcgaa aaactctcat gacttaatga
    10381 atatcttgca tcatcaggat aaggaattga gagggcgttt gttggaaatg atatttacta
    10441 gccaaaacta attgaaataa ttagaaagtc atttgtttag tgtatatgaa gattctgtat
    10501 ggtattcgtt atcactggtc acattgaaaa tgtgaaataa gcacctacaa catagacttt
    10561 acaacacttc atgtttcata tcatggggtg tttttctatt taagtcaaag cccatcaaaa
    10621 aatctcacta ttcatcaggt taaaggaaac ctaatcatag tgagattgtg aattatagta
    10681 gagtttgtat cgcctcttca atttgttttg ggtctgtttt tgaagaaaag cgttcaaaga
    10741 cttgcccatc tcgaccgatg agaaacttag cgaaattcca ttcgattcgt tttcctagtg
    10801 ggccggattt ctggtctttt aaccagacat agagagggtc tgcttcctta ccgttgacct
    10861 taatcttggc aaaacgtggg aaggtggttt gaaaatgtag gctacagaag gcgttgattt
    10921 cctctgcgct gccgggtgct tgtcccataa actgattgca agggaaatcc aatatttcga
    10981 agccctgatc ttgatagcgt tcatagagtt cttgaagtcc ctggtactgg ggcgttaaac
    11041 cacatccagt agcagtgttg acaatcaaga gaactttgcc acgatagcta tccaagggag
    11101 ttgcttggtt gttttggttc aaaacggaaa aatcataaag tgaagtcatg agggcctctt
    11161 ttctttttct acattgtaac agtttttact tttttcgtcg agtcggatgg aagatattgt
    11221 tgaagaataa gccttcaata tgatagaaac cgtttacaaa taactttaaa aatggtatga
    11281 tttaattaaa ccggtttact tagttgctat aaatctctaa tttgagattt agttcctatt
    11341 gattttacaa tatgtttatt ggagtgtata catgcaaaca aaaacaaaga agctcattgt
    11401 gagtttgtct tcacttgttt tatcaggatt tttattaaac cattatatga cagttggagc
    11461 ggaagaaacg actacgaata ccattcagca aagccagaag gaagttcagt atcagcaaag
    11521 ggatacaaaa aatttagttg aaaatggtga ttttggtcag acggaggacg gaagcagtcc
    11581 gtggacagga agcaaagctc aggggtggtc agcttgggta gaccagaaga atagtgcaga
    11641 tgcctcaact cgagtcattg aggctaagga tggggctatc actatctcaa gccatgagaa
    11701 attaagggca gcggttcacc gtatggttcc tattgaagct aagaaaaagt ataaactgcg
    11761 tttcaagatt aaaacagatc ataaagtcgg gattgccaaa gttcgtatca ttgaggaaag
    11821 tgataaggac aagcgattgt ggaattctgc aacgacgtca ggaacaaagg actggcagac
    11881 cattgaagca gactatagcc cgactttaga tgttgataaa atcaagctgg agttattcta
    11941 tgaaacagga actgggactg tttcctttaa ggatattgag ctggtagagg tagcagacca
    12001 gccttctgag gactatcaaa cagataaaca gcttgaggaa aagattgatt taccaattgg
    12061 aaaaaaacat gttttttctc ttgcggacta tacttataag gtagaaaatc ctgacgttgc
    12121 ttcagtcaaa aatggaattt tagaacctct taaggaaggg acaaccaatg tcattgtcag
    12181 taaagatggc aaggaagtga aaaagattcc tttgaagatt ctagcctctg ttaaggatgc
    12241 atacacagac cgtttggatg actggaatgg catcatcgct gggaatcaat actatgattc
    12301 taaaaatgaa cagatggcca aattaaacca ggaattggaa ggaaaggtag ctgatagcct
    12361 atctagtatt tcaagtcagg cggaccgcac ctatttgtgg gaaaaatttt caaattataa
    12421 aatgtctgca aatctgactg ccacttatcg gaaattggag gagatggcca agcaagtgac
    12481 caatccttct tctcgttatt atcaagatga aactgtcgtt cgaacagtca gggattccat
    12541 ggaatggatg cataaacatg tctacaatag tgaaaagagc attgttggga actggtggga
    12601 ttatgaaatc ggtacacctc gtgccatcaa caataccttg tctctgatga aagaatactt
    12661 ctctgatgag gaaattaaaa aatatacaga tgtgattgaa aaatttgtac cagatcccga
    12721 acatttccga aagacgactg ataacccatt taaggctcta ggtggaaact tagttgatat
    12781 gggaagagta aaagtaatag ctggtttact gcgtaaggat gatcaagaaa tttcttctac
    12841 cattcgctcg attgagcaag tgttcaagtt ggtagaccaa ggtgaaggtt tttatcaaga
    12901 tggatcctat atcgaccata ccaatgttgc ctatacgggt gcttatggga atgttttgat
    12961 tgatggcctg tctcaactgt tgccagtcat tcaaaagacc aagaatccaa tcgataaaga
    13021 taaaatgcaa accatgtacc actggattga taaatcgttt gctcctttgc tggtgaatgg
    13081 agagctgatg gatatgagtc gtggacgctc gatcagtcgt gcaaatagcg aggggcacgt
    13141 ggccgcagta gaagtactaa gagggattca ccgaatagcg gatatgtctg aaggagaaac
    13201 caaacaacgt ttgcagagtc ttgtgaagac cattgttcaa tcggatagtt attatgatgt
    13261 ctttaagaat ttgaagactt ataaggatat cagtttgatg caatccttgt taagtgatgc
    13321 aggagtcgca agtgttccaa gaacaagtta cctatctgcc tttaacaaga tggataaaac
    13381 agccatgtac aatgcagaga aagggtttgg atttggcttg tcactctttt ccagtcgtac
    13441 cttgaattac gaacacatga acaaggaaaa taaacgtggt tggtatacga gtgatgggat
    13501 gttctatctt tacaatggcg atttgagtca ctatagcgat ggctactggc caacagttaa
    13561 tccatataag atgcctggta caacagagac ggatgctaag agagcggata gcgatacagg
    13621 taaagtttta ccgtctgctt tcgttggaac gagcaaacta gatgatgcca atgcgacagc
    13681 aaccatggat ttcaccaact ggaatcaaac attgactgct cataagagct ggtttatgct
    13741 aaaggataag atcgcctttt taggaagcaa tatccaaaac acttcaacag atactgctgc
    13801 aactacaatt gaccagagaa aactggaatc aagtaatcca tataaagtct atgtcaatga
    13861 taaagaagcc tcccttacag aacaagaaaa ggattatcct gaaacccaaa gtgtcttttt
    13921 agaatcatcc gattcgaaaa agaatattgg ttactttttc tttaagaaga gttcaatcag
    13981 tatgagtaag gctttgcaaa agggagcctg gaaggatatc aatgaaggac agtcagacaa
    14041 ggaagttgaa aatgaatttc ttacgattag tcaggctcat aagcaaaatg gagattctta
    14101 tggctatatg ctcatcccta acgtgggtcg tgccaccttc aatcaaatga taaaagagtt
    14161 agaaagaagc ctcatcgaaa ataacgaaac ccttcagtct gtttatgatg ccaaacaagg
    14221 agtttggggc attgtgaaat atgatgattc tgtctctact atttccaacc aattccaagt
    14281 tttgaaacgt ggagtctata ccattcgaaa agaaggggat gaatataaga ttgcctacta
    14341 taatcctgaa acccaggaat cagctccaga tcaggaagtc tttaaaaagc tagagcaagc
    14401 agctcagcca caagtacaga attcaaaaga aaaggaaaaa tctgaagagg aaaagaacca
    14461 ttcggatcaa aagaatctcc ctcagacagg agaaggtcag tcaatcttgg caagtctagg
    14521 gttcttgcta cttggggcgt tttatttatt ccgtagagga aagaacaact aatttgttca
    14581 tcatctagat gaattacatg aaactgtgga aaatagaaaa aatccgaaga aggccaaata
    14641 tcttcggatt tttcttttaa aaaaggataa gttgatactt atccttttta cttcattatg
    14701 tgactatagc gttttgaaac tagaatagga catcataact gctaaaagat ttctataaat
    14761 tcatttgatt ttcctaatca atttattcgt atcctatttc actctactat attagtccgt
    14821 ctcgcacctc acggagcgag acaaaacaac cacccgctat gcgggtgcgc gtcgaaggtt
    14881 ataccaaaaa aactccaaac gcgatacaat aaaggtgttc aatccacttg taaagcgaaa
    14941 ggagaaaaaa tatggcacaa aaggctcata gtttatctca cacaaagtgg aggtgttcta
    15001 tcacattgtg ttcaccccta agtatagacg aaaagtcatc tata
//
LOCUS       DAVBBS010000051        14432 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVBBS010000051 DAVBBS010000000
VERSION     DAVBBS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14432
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.051"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..2288)
                     /locus_tag="VQU32_001617"
     CDS             complement(<1..2288)
                     /locus_tag="VQU32_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HET2473044.1"
                     /translation="MFKVMRGGVDTTQVMTETVEDKVSHSITGLDILKGIVAAGTVIS
                     GTVATQTKVFTNESAVLEKTVEKTDALATNDTVVLGTISTSNSASSTSLSASESASTS
                     ASESASTSASTSASTSASESASTSASTSISASSTVVGSQTAAATEATAKKVEEDRKKP
                     ASDYAASVTNVNLQSYAKRRKRSVDSIEQLLASIKAAVFSGNTIVNGAPAINASLNIA
                     KSETKVYTGTGLDSVYHVPIYYKLTVTNDGSKLTFTYTVTYVDPTTRALGNLSKMGHG
                     YSIYNTGTSIQTMLTLGSDLGTPSDVKNYITDKDGVLVRSYNTSTMTTWGSGYTWGNG
                     AQMNGFFAKQGYGLTSSWTVPITGTDTSFTFTPYAAKTDNIRTNYFKGRGKVVESSTT
                     SQSLSQSKSLSVSASQSASASASTSASESASTSASASASTSASASASTSASASASTSA
                     SASASTSASESASTSASASASTSASASASTSASASASTSASASASTSASASASTSASA
                     SASTSASASASTSASESASTSASESASTSASASASTSASASASTSASASASTSASASA
                     STSASASASTSASASASTSASESASTSASASASTSASASASTSASASASTSASESAST
                     SASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSA
                     SASASTSASASASTSASESASTSASASASTSASASSSTSASASASTSASASASTSASA
                     SASTSASASASTSASESASTSAS"
     gene            complement(2286..2399)
                     /locus_tag="VQU32_001618"
                     /pseudo
     CDS             complement(2286..2399)
                     /locus_tag="VQU32_001618"
                     /inference="COORDINATES: protein motif:HMM:TIGR03715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KxYKxGKxW signal peptide domain-containing
                     protein"
     gene            complement(2637..3098)
                     /locus_tag="VQU32_001619"
     CDS             complement(2637..3098)
                     /locus_tag="VQU32_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2473045.1"
                     /translation="MGKERRKQGNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTTSFREQKPEKVSEFLDILDNLKDLPVLYIDETGI
                     NRYLYRPYAGAPRGEKVYDKISGRRFERTNEVEQKLNGSFLIRYIDSQIRE"
     gene            complement(3065..3208)
                     /locus_tag="VQU32_001620"
     CDS             complement(3065..3208)
                     /locus_tag="VQU32_001620"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2473046.1"
                     /translation="MAYSTDFKQGALDSIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KGT"
     gene            3477..3857
                     /locus_tag="VQU32_001621"
     CDS             3477..3857
                     /locus_tag="VQU32_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2473047.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            3956..4177
                     /locus_tag="VQU32_001622"
     CDS             3956..4177
                     /locus_tag="VQU32_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET2473048.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            4194..4508
                     /gene="trxA"
                     /locus_tag="VQU32_001623"
     CDS             4194..4508
                     /gene="trxA"
                     /locus_tag="VQU32_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET2473049.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            4815..5348
                     /gene="queF"
                     /locus_tag="VQU32_001624"
     CDS             4815..5348
                     /gene="queF"
                     /locus_tag="VQU32_001624"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HET2473050.1"
                     /translation="MKHQSALVVFSGGQDSTTCLFWTMQHYETVEAVTFAYGQHHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            complement(5602..6270)
                     /locus_tag="VQU32_001625"
     CDS             complement(5602..6270)
                     /locus_tag="VQU32_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HET2473051.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLSN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(6381..6935)
                     /locus_tag="VQU32_001626"
     CDS             complement(6381..6935)
                     /locus_tag="VQU32_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HET2473052.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRNVLLNQGL
                     HYLSKEKDTRGFSSQYGWVHAFAHGADLLTEVVCHPGFPKNRVHEVFEILGQLFKRMS
                     IRFTDDEDWRLARVIYEPILQGKLAQEQVASWIKTVDFPIEEREDFYKFSNFRSCLVE
                     VYVQLDQRNSLQDELKEAIQSFQY"
     gene            complement(7181..8977)
                     /gene="pepF"
                     /locus_tag="VQU32_001627"
     CDS             complement(7181..8977)
                     /gene="pepF"
                     /locus_tag="VQU32_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET2473053.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYGFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVAADEEVLDRLGKLPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDATLTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKHSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIISYSAELGE"
     gene            complement(8988..9731)
                     /locus_tag="VQU32_001628"
     CDS             complement(8988..9731)
                     /locus_tag="VQU32_001628"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET2473054.1"
                     /translation="MQQYFVKGSAVSPVTIEDRETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFITQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNVVPSIHLFENKADFLAQLDQ
                     FDRILVAYEEAAKEGEAAALIKAVTGLEKGAKLLFIFGPEGGLSPAEIENFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            complement(9733..10683)
                     /locus_tag="VQU32_001629"
     CDS             complement(9733..10683)
                     /locus_tag="VQU32_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET2473055.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYMGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDMTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNTG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDKW
                     DMVRQSAESAGFFLETHMIQGEWNACVFKKTKDISGVIGG"
     gene            complement(10821..11249)
                     /locus_tag="VQU32_001630"
     CDS             complement(10821..11249)
                     /locus_tag="VQU32_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2473056.1"
                     /translation="MELEISDFQGCKIALFCGDKLLTILRDDKDDIPCPNMWELPGGG
                     REGDESPFECARREVYEELGIHLDEDCLLWSKIYPSVIFKGKKSVFMVGQLRQEQFDN
                     IIFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            complement(11230..12318)
                     /locus_tag="VQU32_001631"
     CDS             complement(11230..12318)
                     /locus_tag="VQU32_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET2473057.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            complement(12337..12807)
                     /locus_tag="VQU32_001632"
     CDS             complement(12337..12807)
                     /locus_tag="VQU32_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HET2473058.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     VLFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            complement(13098..13550)
                     /locus_tag="VQU32_001633"
     CDS             complement(13098..13550)
                     /locus_tag="VQU32_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HET2473059.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            complement(13587..13766)
                     /locus_tag="VQU32_001634"
     CDS             complement(13587..13766)
                     /locus_tag="VQU32_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HET2473060.1"
                     /translation="MPMTQKEMVKLFTAHGWIKTRGGKGSHIKIEKQGERPITILHGE
                     LNKYTERGIGKQAGL"
     gene            complement(13903..14082)
                     /locus_tag="VQU32_001635"
     CDS             complement(13903..14082)
                     /locus_tag="VQU32_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473061.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            complement(14247..>14432)
                     /locus_tag="VQU32_001636"
     CDS             complement(14247..>14432)
                     /locus_tag="VQU32_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473062.1"
                     /translation="IFTKQIRHWILSDQVISGKRKLFFREDTPKEILDLYENIKSKLD
                     FAYQEVHSNNGLKKYEK"
BASE COUNT         4196 a         3287 c         2765 g         4184 t
ORIGIN      
        1 gacgcactcg ttgaggctga ctcagacgca ctcgttgagg ctgaagctga cgcactcgtt
       61 gatgctgaag ccgaggcact ggttgacgct gaagctgagg cgcttgttga tgctgaggct
      121 gatgcgcttg tgcttgatga agctgaggca cttgttgatg ctgaggctga cgcactcgtt
      181 gatgcagatt cagacgcact tgtgcttgct gaggctgacg cactggttga tgcggaagct
      241 gaggcgcttg tgcttgctga agccgacgca ctcgttgagg ctgaagctga agcgcttgtc
      301 gatgctgagg ctgacgcact tgtcgacgct gaagctgaag cactggttga cgctgaagct
      361 gaggcactgg ttgacgctga agctgacgcg ctggttgagg ctgaggctga ggcgctagta
      421 cttgctgact ctgatgcact ggtacttgct gaggctgacg cacttgtgct tgctgaagcg
      481 gacgcactcg ttgatgctga agctgaagca ctggtacttg ctgactctga tgcactggta
      541 cttgctgagg ctgacgcact tgtgcttgct gaagccgatg cactcgttga tgcggaagct
      601 gatgcacttg ttgatgcgga agctgatgca ctcgttgatg ctgaagctga ggcgctagta
      661 cttgctgagg ctgaggcact ggttgatgcc gattcagatg cactggttga ggccgattca
      721 gacgctgagg tacttgctga agctgacgca ctggttgacg ctgaagctga tgcactggtt
      781 gacgctgagg ccgaagcact ggtacttgct gaagccgacg cactggtgct tgctgaagcc
      841 gaggcgctag tgcttgctga agccgacgca ctcgttgatg cggaagctga cgcactcgtt
      901 gatgcagatt cagacgctga ggtacttgct gaagctgagg cactcgttga tgcggaagct
      961 gacgcactcg ttgatgctga ggctgaggca cttgtacttg ctgaggctga tgcgctggtt
     1021 gaggccgatt cagaagctga ggtgcttgct gaggctgagg cgctttgact agcacttact
     1081 gagagtgact tagactgtga aagtgactga ctggtcgtgc tagattcaac tacctttcct
     1141 ctacccttga agtagttagt tctaatgtta tctgttttag cagcgtaagg ggtaaatgta
     1201 aaggatgtat ccgttccagt aattggtaca gtccaagatg atgttaatcc atatccttgc
     1261 ttagcaaaga aaccattcat ttgggcacca tttccccaag tatacccact accccacgtc
     1321 gtcattgtag atgtattata ggatcgaacc agtacaccat ctttgtcagt aatgtagttc
     1381 tttacatctg aaggggtacc aagatcactg ccaagggtta acattgtttg tattgaagta
     1441 cctgtattat agatagaata cccatgaccc atctttgaaa gattaccaag agcccttgtg
     1501 gtaggatcta catacgtaac cgtataggta aaggtcagtt ttgaaccatc atttgtcact
     1561 gtcaatttat agtaaattgg aacatgatat accgaatcta aacctgtacc tgtataaact
     1621 tttgtctcac ttttagcaat gtttagactt gcattaattg caggggcgcc atttacaatc
     1681 gtattgccag aaaaaacagc agcttttata gaagccagca attgctcgat ggaatccact
     1741 gaacgcttgc gtcgcttagc ataagattgg agattgacat ttgtaactga tgctgcataa
     1801 tcactagctg gtttcttacg atcttcttcg accttcttag cagttgcttc tgtagcggca
     1861 gctgtttgtg atcctaccac agtagatgat gcagagatac ttgtcgaagc cgatgtactt
     1921 gctgattctg atgcacttgt actcgctgag gtcgaagcgc tggttgaggc tgactcagat
     1981 gcagatgtac ttgccgactc tgaagctgac aaactagttg aactcgctga attacttgta
     2041 gatatcgtac ctagaactac tgtatcattt gttgccaaag catccgtttt ctctacagtt
     2101 ttttcaagta ctgccgactc atttgtaaat accttcgttt gagttgcaac ggttccactt
     2161 atgacagttc ccgcagcaac tattcccttg aggatatcaa gcccagtaat tgaatgactt
     2221 actttatctt ctaccgtttc ggtcatgacc tgagtagtat caacaccacc acgcatgacc
     2281 ttaaacaagc caaaaagaga agtcgaggcc cgtagccaat gcttccctga tttgattaat
     2341 ttgaaccgag ttactcggtc tgtttctcta tattcaccct tctgacgtct aaaaaatatt
     2401 ttgaaatttc ctttcgagta ttattttcat gtatggatat aaataaatta ccatactata
     2461 attatagaat agggaaccca aaatagcaag aaacttgagg tatttttgct aaaaaaaaaa
     2521 agcattttac tattttatat atatattata agataagttt tttataaaga cacgcaaatg
     2581 agtataaatc agctaatttt tggtttatat tcctattttt atgcataatt attctttcat
     2641 tctctaattt gtgaatcaat atatctgatt agaaaactac cattgagttt ttgctccacc
     2701 tcattagtcc gctcaaaacg ccgtccgcta atcttgtcat agactttctc ccctctagga
     2761 gcccctgcat aaggacgata gaggtagcgg ttgattcccg tttcgtcaat atataggact
     2821 ggtaaatctt ttaggttatc caaaatatca agaaactcag aaactttctc aggcttttgt
     2881 tccctaaaac tggtcgtctt ttttaaaatg accttaatct gctttaaaac tgcccatacg
     2941 gagggcaaag cacaatcaaa acgggccgca atttcccgta aaaaagcgtc tggatgagcc
     3001 tctacaaagg ctttcaattc ttctaaaggg atcttacgct ttttgacgac tcgctttttc
     3061 cgctctaagt tcccttgttt acgtctttct tttcccacgt gaagagagtt ctgacgccaa
     3121 caccaaaaaa cttggctgcc tcgacatgac tgtgcccctc tttgatggaa tctaatgctc
     3181 cttgtttaaa atcagtacta tatgccatag ctttattaga ccatgcttat tttaaactaa
     3241 gcaactatag atagagaaat gaaataaaat ctaaattcga atgtgataag gagttagaaa
     3301 ttgaaacaat aggattcccc cattattcac acaaaaataa aaccactaaa aaacagagct
     3361 tgccagcatt cttgaaaagt agtataatta ttgcatgcgc aatcatcttg tgacgcagag
     3421 actgcccgtt aatggacttt cttctatatt atgactctaa aaagaaagga gacactatga
     3481 cctatttgga aaaatggttt gacttcaatc ggcgtcagaa agaaattgaa agtctcttgg
     3541 aagagaccat tgcccaacag agcgagcaaa gtctgacctt gaaagagttc tacctgctct
     3601 actatctgga cttggctgaa gaaaaatctc tacgccagat tgacctgcca gataaacttc
     3661 atctgagccc gagcgcagtt tctaggatgg tggctcgctt ggaagctaaa aattgcggtc
     3721 tactcagtcg catgtgttgc catcaagata gacgctctag ttttatctgc ctgacaaatg
     3781 atgggcaaaa gacactggcc tcgctacaaa agactgtcga agaaagcttg gaagcaggtt
     3841 tggatttctt gatttaagat gattttaaaa aaagttgcgt gcgcaatcat ttttcttgac
     3901 attctctttt ataaggagta aaataaagtt agcattaaac aaaggagttt taaatatgat
     3961 tgaaattacc tatatagatg ccagcaagaa cgaaagaact gtgactttcg agtcttatga
     4021 agactttgaa cgttcacaac aagcttgcct tatcggcgtc gcagactact accctgtcca
     4081 aaaattaact tacaagggtc ataatttgga ctaccatggg acttatggag atatcttctt
     4141 ctatctcatg aaacaagatt taagccaata taactaaaaa aaggagaaat acaatggcaa
     4201 aagcaattac agatgcaaca ttcgaacaag aaacaaaaga cggtttggtc ttagtagact
     4261 tctgggcaac ttggtgtggt ccatgtcgta tgcaaggtcc aatcttggac aaattgtctg
     4321 aagaactttc agaagatgtc ttgaaaatcg ttaaaatgga cgttgatgaa aatccaaaca
     4381 cagctcgtgc ttttggaatc atgtctattc caactcttct cttcaaaaaa gacggccaag
     4441 ttgtcaaaca agttgcaggt gttcacacag cagaacaaat caaggccatc attgctgaat
     4501 tgagctaatc aaacgagaga ccaagtactt tctttggtct cttttttctt gccctttgcc
     4561 atttttcaaa aaatatgcta gactatagta gattgaaact agaatagtac acctctactt
     4621 ctaaaacatt gttaaaaatc gatttgactg tcctgatcga tttgtcctgt ttttatttca
     4681 ttttactata taagtagaat attacgatgt ttgggatatg ccatcgctaa aaaaaaactc
     4741 gatttggtat tttttagttc cctaatggga gctttttgcg tgctctgaac atttcccatt
     4801 ttggaaggag tactatgaaa catcaatcag ccttggtcgt ctttagtggc ggtcaagatt
     4861 caacaacctg ccttttctgg accatgcaac actatgaaac agtcgaagct gtcacctttg
     4921 cctacggcca acatcatcac ctcgaaattc aaattactag agaaatcgct aaggaacagg
     4981 gcattcgtca ccatatcctc gatatgtctc tgctgggaca aatcactgct cagccagact
     5041 ttgcgacgat tcatatttcc tacattcctg acaagctctg tgtcgagtca aaatccctca
     5101 aactatatct atttagctac cgaaaccacg gagatttcca cgaaaactgt atcaacacca
     5161 tcgggaaaga cttggtcaac ttgctagacc ctcgctattt agaagtctgg ggaaaattca
     5221 ctccgcgcgg tggcatttca atcgacccct actataacta cggtaagcaa ggaactaagt
     5281 atgagggctt ggcagaacaa cgcctcttcc aacacgacct ttatccagag aaaattgaca
     5341 accgctaaag tcatactcaa tgaaaatcaa agagcaaact aggaagttag ccgcaggtta
     5401 ctcaaagcag tgtttgaggt tgtagataaa actgacgaag tcagatcaaa acactgtttt
     5461 gaggttgtgg atagaactga cgtagtcagt aaccatacct acggcaaggt aaagctgacg
     5521 tggtttgaag agattttcga agagtatcat acgaaaaagc cctgttcctc aagatgagga
     5581 gcaaggcttt ttgagtttca attattcttc tgttccaagg aaattttttg caacaagggc
     5641 tgcaagaact ccaccagcga ttggtgcaag gatgaaaatc caaacttgtt gaagggctgc
     5701 gccgcctacc aagacagctg gtgccaagct acgagctggg tttactgaaa gtccagtaat
     5761 cttcaatccg acaagaatca tcgccatcaa tgacaaaccg attaccaaac cagcaatcgc
     5821 gccattgccc ttgctttctg aagtcacagt catgataacc aatacaaaca agaaagttgc
     5881 gatgacttca aacaagaaac caccaaagac agtgacaccg tttgccaagg cattttcacc
     5941 aagactagca gttgacatac ctgaattact caagaggaag aagactgcgc cagaagcgat
     6001 gaaagctcca acaacctgac caaggatgta gtttacaagt tctgaagatg acaaacgttt
     6061 gtttacaaac atagcaatcg aaacagccgg gttcaagtga gcacctgaaa cagttccgat
     6121 tgagtaggct gccaccacga ttgccaaacc aaaggcaaag gcgattccaa ggtgaccaag
     6181 gccatcaaga ccatttccaa aaacaacagc tcctgttcca acgaacacaa gcatgaacgt
     6241 accgattaac tcagcaacaa attttttcat tttctttctc cttttttcaa aaactagata
     6301 ctagtctatc aaaagtagga aagggtttca agaaaattga ttggaaattt tttgaaaatc
     6361 atagaactat tagctaatct ctagtattga aaagactgga tagcttcttt caactcatct
     6421 tgtaaactat ttctctggtc aagttggaca tagacttcca ccagacagga tctaaagttg
     6481 gaaaatttgt aaaaatcctc cctttcttct atcggaaaat caacagtttt tatccaagaa
     6541 gctacttgtt cttgcgctaa cttcccttgt aaaataggtt catagatcac tcttgctaaa
     6601 cgccaatcct catcatctgt aaagcgaatc gacattcttt taaataattg gccaagtatc
     6661 tcaaatactt catgaactct gtttttagga aagcccggat gacaaaccac ctctgtcagt
     6721 aaatcggctc catgtgcaaa agcatgaacc caaccatact gacttgaaaa accccttgta
     6781 tccttttctt ttgaaagata gtgcaagcct tgatttaaaa ggacattacg aatttctgga
     6841 gaaggatttc ccaaatgatc aaacaaccac tggatttctt cctggttata atttggtttt
     6901 tcttctgcta tttttcttag taaatcttga tacatggtca atacctctac atttctagca
     6961 actgttcaaa aaggcagtct taaatgactc aatattgaat tctcaattaa atacaatctg
     7021 atataaaatg acgtaaataa ctatcaatac cagttctaca gtaagttcaa atttaacatc
     7081 acgatcttca acgacatttt tgaaaatagc tacaactaag acaaatagaa tgacgcttaa
     7141 caagcccata aacatcattc taaaaaattt ttctattccc ctactctccc aactcagcac
     7201 tataggagat aatctggtca actgtgtcag acaagaattg gatggtatca cggagtggtt
     7261 tgtctgttga aatatcagca ccgataatca tggctgactc aagtggtgtc ttgctaccac
     7321 ctgatttgag gagattgagc cagtcttcag ctccagtttc agaatgtttt agatgaaggt
     7381 aaccagcagt cgagataact agtcctgctg agtaagtgta actatacaag cccatatagt
     7441 agtgagcttg gcgcatccaa gtcagagttg catcatcgtc aatttcaata gcatctcccc
     7501 agaaatccgt caaaacttcc ttcataatgc tgttgagctt gcttgctcca aaggtctccc
     7561 cttcttcaat caatgtatac accttacgct ggaaggcggc ttccaagagg tgggtgataa
     7621 agttatggaa gtaggtgtct gtcaagcgat gagccagagc gaagcgtttt tgacgtgggt
     7681 cattagactg gttctccaag taatcactga gtagcaattc attgaaggtt gacggtgctt
     7741 caacatagta ggtcgacata tgggcattga agtaactttg atgattgtct gaaaagatga
     7801 attgaccaga atgcccgatt tcatgaatca aggtatagac atcgctcaaa cggcctgtcc
     7861 agctcatgag tacataaggg tgtacgcgat atgggtccgc cgcataacca ccggaatcct
     7921 tgccactgtt agcagcaaag tccacccagc gctcttcttg gtaacgagca acttcctgac
     7981 aatattcttg ccccaaaggt tctaccgact tcatgaccaa atcataggca tcgtcaatag
     8041 tcacttcagg attcagggcg ctgtccaagt ccaatttcca gtctgcaaag gtcatctttt
     8101 caagaccatt taccttggca acatgcttga ggtatctctg agcgactggt gcaaagtcct
     8161 tcatgatgag gtcaatctgg cggtcaaaca tgacacggtc cacttcttgt tcagctagaa
     8221 gatagtcaaa gacagagtcg tatcccttca tatcagccaa gagtttttca gacttgacct
     8281 gagccagata ggctgctgca gccgtatttt ggtgcttacg aagtccctct gagaaggaac
     8341 ggaaggattt ctcacgaacc tcagcatcct catggttttg gtagaaattc tcataggtca
     8401 caaagctgtt tttgtaggtc ttgccatggg cttcaaagtc agccatttca aaatccccag
     8461 ctcgcatctt agtataaatg tcctgcggac tgtagaaaac ttcaccgaga tttgtcaagg
     8521 ccttctccac atctgcccct aagtagtggg cttttttgat tttagcctga cgaatggcag
     8581 ctgttaaatg tggcaattta cccaaacgat ccaagacttc ctcgtctgct gccaccaagg
     8641 catcgtcaaa aaaggtcaag gctacgctgg catctgtttc aaattccatc ccagcttgag
     8701 caatatttgc aaattcgtca ttgctatagt ccgtcgtctg aggcataaaa ccatagttgc
     8761 caatatggct catctgaatg tagatctgtt ccaattccgc aaaggccttc tcgaaatcct
     8821 caaaagtgtg aagattgccc ttgtaatcac ggctaaactg gttgatgtct tcgcgagctt
     8881 tctcgattgc acgcaagaaa tcctcacggt cttggtatag agctgttaaa tcccaaagtt
     8941 ccttctctgg aaattctgaa cggtgttttt gttccatttt cttcctctta tttctctaat
     9001 tctactaaaa cactaagggc tgataaggcg taaagcggtg ctgtttctgc tcttagaatg
     9061 cgagggccaa gtcctgccaa aacagctccc ttagcttcaa aattttcaat ttccgcaggt
     9121 gacagaccac cttctggacc aaagataaag agcaatttgg ctcctttctc gagtccagta
     9181 actgctttta taagagcagc ggcttctcct tctttggccg cctcttcata ggctactaag
     9241 atgcggtcaa actggtcaag ttgggctaga aagtctgctt tattctcgaa aagatggata
     9301 cttggaacaa cattacgctt actttgctct gctgctccaa gagcaatttt ttctagtttt
     9361 tcaacctttt tacccaattt cttgccatcc cacttagcaa cggaccaatc ggcagggaag
     9421 gcccaaatct ggctggcacc tagttccgtc accttttgag tgataaactc caacttatct
     9481 cccttgggaa atccagatgc gatggtcact tggactggta gttccacatt gtcatttaat
     9541 tcttggacca actcaaactg acgattttcc atatccagca cgcgcgccaa gcgcttgatg
     9601 ccatcatcaa agactaaggt aacctcatcc tcttctttca agcgcataac ctgaaacata
     9661 tgcttgctgg tttccctgtc ctcgatggtg acaggagaga ctgcactccc cttgacaaaa
     9721 tactgttgca tgctagcctc caatcacacc tgaaatatcc ttggtcttct tgaagacgca
     9781 ggcattccat tccccttgaa tcatatgggt ttcaaggaaa aatccagctg actcagccga
     9841 ctggcgcacc atgtcccact tgtccttgat aatgccactc atgatgaggt agccttcatc
     9901 ctttaccaag cgataagcat cctccgtcag atgaatgaga atatccgcca agatattagc
     9961 cacgatgaca tctgcttcaa tctcaactcc cttaagcaag tctcctgctg ctacatggat
    10021 attttccata ccagtgttga gctcaatatt ttcctgagcg acacgaaccg ccacatcatc
    10081 caagtcatag gcgaaaatct ccttggcacc taggagtgag cttgcaatag aaagaacccc
    10141 tgaaccagtc cccacatcta gcaccgtttc gccaccacga agaacctgtt ccaaggcaaa
    10201 aaggctcatc ttggtagttg ggtgggttcc agtaccaaaa gccatgccag gatccagctt
    10261 gataattttt tctcccgcag tcgcctcata gtctgtccaa gagggaacga tggtcaaatc
    10321 atgagtgatg cgagcaggtt catagtattt cttccagttg tctgcccagt cttcctcagc
    10381 caaggcagtc gtacctattt ttaactctcc caaatccata aaatccgtca attctgctaa
    10441 gcgagcctgc aagtccgcct caaccactgt catatccacc gtgtcagggt agtaggctgt
    10501 cactacgatt tcttcttgct gctccacctc tgggaaaatc tcgccaaaac gatccacatt
    10561 tcccatatag tccatgctgt cttcaatcgc gactccttgc gctcccagct caatcaagag
    10621 attggaaacc aactcctctc cctcacgctt cactgtaact tttaactctt gccatgtttc
    10681 cattattaag ataccaagcc cgtaaaacac aaaaccaaaa taggaaattc tctgaagacg
    10741 cttgtgtcta agagaagttt atctttttgg cacagtgttt agggcgggtt cagtttagaa
    10801 atgtaactga accatccttt ctaatcactt acttttaaat aatcttttaa tctctcttgc
    10861 aactgaggca caacttgact ggaactaaga aattcctcaa cattcatcag ctgatagccc
    10921 tgtccttcat ctccgaagat gatattgtca aattgttctt gtcttagctg accaaccata
    10981 aagaccgatt tcttgccttt aaaaattacg ctaggataaa tcttgctcca aagcagacag
    11041 tcttcatcta aatgaattcc cagttcctca taaacttcac gccgagcgca ttcaaaaggg
    11101 ctttcgtccc cttcacggcc accacctggc aactcccaca tgttgggaca agggatatcg
    11161 tccttgtcat cacgcaaaat cgtcagtagc ttatccccac aaaagagggc aatcttgcaa
    11221 ccttggaaat cagaaatttc tagttccatc ttcagttcct tctaacattt ccttttccag
    11281 ctctgctaac aagttttcat aatagcgttt ctcgtccctc aacattcgac tactatccat
    11341 tttctgtcta gcaatcttga gagccttacg agttcgatct acatctttct tcacctttaa
    11401 ttgataccag gcttgtatca cttgaagatt ggacagtttg agagacagaa acgatttgac
    11461 ctgtcgaata ctagcatatt gctccgcttg ctcaaaatct ccttccaaca aggcgatatg
    11521 aagcagggat agttgggcaa ctgtctgcat catcggagta gttgtcctct caagtaatgc
    11581 ttgaaactgc tgtttagcta cttcttcctt cccttccaaa atggaaactt caccttgcat
    11641 acctaataca ccatccgcaa aactccctcg tgcatcctca ggaactgctt gaacaaagtc
    11701 tttcaaatca tattcttgag gagccagcaa agtttgagca gaatgtctca ataccaggta
    11761 ggcgtatttg gtattttcag ggtgttgtag taattcccaa atttttgctc catcggtgat
    11821 gtcgactggc aaaatgttat ttaggaagaa agataaatta agaaaaatcc aagtccctgc
    11881 aaaataccag cttcttgtca aaaatccaaa cactatcgcc aataatatca agccgagatg
    11941 aaccatcaag cctcctgaaa gcatcaggat gattctttga tcgctttcat cctcttttaa
    12001 accaatgtat tgagcaccaa catttttcag aatggctgtt ctactaagat gaaacctgcc
    12061 tgactttttg gtcaaaataa aatgtcctaa tccaaaagcc accagccgat agcctgtcaa
    12121 gtagccacaa aaagcatgac ccagctcatg aagaataaag attaaataca tgcttagaag
    12181 agcgaaggca taaccaaaag taaaggctaa aactgcggaa taccccaact ctgcaaatgc
    12241 gattgttcca caagcaaaag ctagcataat aaagacaaca gctagcacat aaaccaaata
    12301 agtcccaatt ttcttcataa cacctccaac caactcctag tatcttggat aaggataaaa
    12361 ttctccctct tccaagccaa cttttccttc ttcaaagact tcttggttcc attccatgac
    12421 aaattcctct gcttctgggt cttccaaaaa gtccatgagg acatctagcc caacctcagc
    12481 agtatcttta aggaaaagca caaaataagc taaaaattca cgggaaaatc cttttttagg
    12541 caggtaagga ataacagtca aatagtcttc ctcattgact gttgacttag caggattgta
    12601 gaaaaggacc gcttcctcaa aaagaatgtc atctgatgaa acctctccgt cttcatccac
    12661 catctccaca cctgcagcat tttgcgcttc caatagaaaa ctcacttcta ccgcatggtt
    12721 gcgtttgtcc cagctaatct caaagtcaaa gggaaagttc ttgtccaact cttcctctaa
    12781 aacatctaaa aatccgtatg ttgccatttt gtcctctttc tatgcgactc tttaatcgcc
    12841 ccgattgctc ggaaatatgc taaaatagat actaccatct taccacaaaa ttattttatg
    12901 tcctaattat accatattac ctcatttaaa cccttggtat cagtgatttt cttaaaagtc
    12961 tgatttcttc atttctcata aaaatcaata taaaaagccc tcgaaagggc taataaatct
    13021 ataaaatcaa taggcgagta actagcacaa gtggacgtgc ttttttattg actattacca
    13081 cgataccacg cttaatctta ggcttgaact ttcttatctg caatagcgtc tgtcaaagtc
    13141 tgagaaaagt taagtcccat ttctcgtccc aacttatctg cccattttgg tatggtcaaa
    13201 gtctttttaa tgggttcctg acttcctagg tattctgata catcaacaga taccatagaa
    13261 ataaaagatt tatcaaggtc ataggttgac acgaaatctt catcatcttt aaaaggatca
    13321 ttatcaatta aagacaagct attgatatct gatggctgag gtaactctcc atcactctct
    13381 atcaaatctg caacagttat ccctagccac tccgacccca tagccaaagc ctcagaaatc
    13441 ccctctcctt gtgtagctga gtattcaaaa tctgggaaat ggacaaaata agtcgcttct
    13501 gttccgtctg tgtcgtcata ataaaataaa gctggatacg taactaacat ttcactacct
    13561 ccatatcaaa aagcagggac tgaattttac aacccagctt gctttcctat ccctctttca
    13621 gtgtacttat tcagctcacc atgaaggatt gtgataggtc tttccccttg cttctcgatt
    13681 ttaatatggg agcctttacc gcctctagtc tttatccaac catgggccgt aaagagttta
    13741 accatctctt tttgtgtcat aggcatagcg cttttacctc ctgacaacac cattataaca
    13801 cgtgttacac gtattgtaaa ggagtgatac ttattattct attatacata aaagccccta
    13861 gatgtggttc taagggaaac caatttattc agacctattt ttctaatgag tagtaaaaac
    13921 tgcttcttta tcgagcaatt catcatctgt atagtcaatt gtaaaagtat ctcgatctaa
    13981 gacagattga ggcggagttg aatgaatcat aggaccactg cgtactctat attttttatc
    14041 tccaattttt acaaactgat actcttcgaa aatcaaattc aaaccacgtc aacgtcgcct
    14101 taccgtactc aagtacagcc tgcggctagt ttcctagttt gctctttgat tttcattgag
    14161 tatgattaac tctcaagtct tcgaaatcag gattttcaac agttattaca aggaggcgat
    14221 ttactacttc aaaaacatca attattctat ttttcatatt ttttcaaccc attattagaa
    14281 tgaacttctt ggtaagcaaa atcaagttta gatttaatgt tttcgtacaa atctaaaatc
    14341 tcttttggag tatcttcccg gaagaaaagt tttcttttcc ctgaaataac ttgatcacta
    14401 agaatccaat gacgaatttg ttttgtaaaa at
//
LOCUS       DAVBBS010000052        14416 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVBBS010000052 DAVBBS010000000
VERSION     DAVBBS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14416
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.052"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(10..1299)
                     /locus_tag="VQU32_001637"
     CDS             complement(10..1299)
                     /locus_tag="VQU32_001637"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET2473063.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVTLPIENALDVYIA
                     VLGEGANIKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(1377..2624)
                     /locus_tag="VQU32_001638"
     CDS             complement(1377..2624)
                     /locus_tag="VQU32_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HET2473064.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGVSIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVSVIISSLTGNDYFGKQSLPRLMMWETVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(2815..3665)
                     /gene="rgg"
                     /locus_tag="VQU32_001639"
                     /pseudo
     CDS             complement(2815..3665)
                     /gene="rgg"
                     /locus_tag="VQU32_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398326.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes.; frameshifted;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
     gene            3789..4130
                     /locus_tag="VQU32_001640"
     CDS             3789..4130
                     /locus_tag="VQU32_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET2473065.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            4188..4268
                     /locus_tag="VQU32_001641"
                     /pseudo
     CDS             4188..4268
                     /locus_tag="VQU32_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(4474..6177)
                     /gene="ilvD"
                     /locus_tag="VQU32_001642"
     CDS             complement(4474..6177)
                     /gene="ilvD"
                     /locus_tag="VQU32_001642"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET2473066.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(6410..7342)
                     /locus_tag="VQU32_001643"
     CDS             complement(6410..7342)
                     /locus_tag="VQU32_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HET2473067.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATNNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(7339..8196)
                     /locus_tag="VQU32_001644"
     CDS             complement(7339..8196)
                     /locus_tag="VQU32_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET2473068.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(8200..9546)
                     /locus_tag="VQU32_001645"
     CDS             complement(8200..9546)
                     /locus_tag="VQU32_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2473069.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMVIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSATEE"
     gene            complement(9559..9843)
                     /locus_tag="VQU32_001646"
     CDS             complement(9559..9843)
                     /locus_tag="VQU32_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2473070.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(9847..11877)
                     /locus_tag="VQU32_001647"
     CDS             complement(9847..11877)
                     /locus_tag="VQU32_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET2473071.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NSHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            12069..13076
                     /locus_tag="VQU32_001648"
     CDS             12069..13076
                     /locus_tag="VQU32_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET2473072.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            13079..13279
                     /locus_tag="VQU32_001649"
     CDS             13079..13279
                     /locus_tag="VQU32_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2473073.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            13431..13613
                     /gene="rpmF"
                     /locus_tag="VQU32_001650"
     CDS             13431..13613
                     /gene="rpmF"
                     /locus_tag="VQU32_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET2473074.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKITKAASAE"
     gene            13629..13778
                     /gene="rpmG"
                     /locus_tag="VQU32_001651"
     CDS             13629..13778
                     /gene="rpmG"
                     /locus_tag="VQU32_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2473075.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(14270..>14416)
                     /locus_tag="VQU32_001652"
     CDS             complement(14270..>14416)
                     /locus_tag="VQU32_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2473076.1"
                     /translation="YLNESGSMATGWVTVSGKWYYTYNSGDLLVNTTTPDGYRVNANG
                     EWVG"
BASE COUNT         4645 a         3043 c         2488 g         4240 t
ORIGIN      
        1 ctttgatttt tattgagtat aaaatcctag tttttcaaag atttctgaga agttttggct
       61 gattgtctca agtgacactt gcacttcttc tcgggtttgg ttgttcttga ccgtcacttg
      121 tccgctttcg acttcgctct ctcctagggt gatgagggtc ttagccgcaa agacatcggc
      181 tgacttgaac tgagctttta gtttacggtt gaggtaatca cgctctgctt tgaaaccttg
      241 ttggcgaaga gcctgtacca attccaaggc cttgatattt gccccttcgc ccaagactgc
      301 gatatagaca tctagggcgt tttcgatagg gagggtcaca ccttgctttt caaggatgag
      361 aagcaggcgc tctacaccaa gtccaaaacc aaatccagca gtttcagggc ctccaaagta
      421 agcaaccaaa ccatcgtagc gaccacccgc acagacggtc aggtcattgc cctcaatctc
      481 tgtgataaac tcgaaaatgg tgtggttgta gtagtccaga ccacgcacca tattggtatc
      541 gatgatgtaa tctactccaa gattttccaa catctgacgc acagcatcaa aatgagcttg
      601 gctttcttca tcaagaaagt ccaagataga cggcgcattc tctactgcca ccttgtcttc
      661 tttttcctta gagtccaaga cacgaagagg attttcctcc aagcgacgtt ggctatcctt
      721 agacaaggtc tccttgagcg gtgtcaaata gtcaatcaag gcttggcggt aggctgcacg
      781 gctctcagga tttccaagag tgttgaggtg caatttgaca ccttgaatac cgatttcctt
      841 caaaaaatgg gctgccatag cgattgtttc cacatcggta gctggattgc tagagccaaa
      901 acactcaaca ccaatctggt ggaattggcg caagcgccct gcctgtggac gctcataacg
      961 gaacataggt cccatgtagt agaacttgct tggcttttgc acttctgggg cgaaaagttt
     1021 attttccaca taggaacgga caacgggtgc agttccttct ggacggaggg taatatgacg
     1081 gtcacccttg tcataaaaat cgtacatttc cttggttacg atatccgttg tatctccgac
     1141 agagcgactg ataacctcgt aatgctcaaa aataggcgtg cgcacttctg catagttgta
     1201 gcgtttgaaa atctcacggg caaagccctc aacgtactgc cacttagcag actcagcagg
     1261 taaaatatcc tgcgttcctt ttggtttttg taatttcata gggaatcctc tttaaactta
     1321 atagtcttat tttaccataa atagagggat taaaacagta agaaaaaaat taggatttag
     1381 atatcatttt tgagattaag aattgtcaaa aaaatagcta gcaaggaaag accaacaaat
     1441 agcatccaag tcaactgtat attccatacg gctactagtg aaaaacaagc tgttcccaca
     1501 ggtatggata aggtaaacaa tagacctaaa aaattactag tacgagctag aacctctgga
     1561 gctagatttt tcatgagcat ggcactaatc tttggttgaa ctttaccaga cacatacaga
     1621 gtaaagaaga gaaatagcaa accaagcacg acttgattga ataaattagc caaaccaact
     1681 agactaagtc ctacggtctc ccacatcatc aatctaggca aggactgctt cccaaaataa
     1741 tcattgcccg taaggctact gatgatgact gatactaaaa cacagaattg attgataaat
     1801 agtgcctctg tataagaaaa attcaagaga gaatggctca aaaagaagat attataaatt
     1861 ccacccaaag cgccacccaa ggaattaata agcaagacag caaagagcat aaaaccaaag
     1921 tttttctgtc cacttttaag aaaaacgaga cgtaaatttc ggtaaattgt taggaactgg
     1981 tctttgatag aaagcttctc attttttaag ttttcaccat cagcagatga cattgacagg
     2041 ctcaatttgc tttttcctaa aaagaggata gtggctgata ctaggaaaaa gcaggcattg
     2101 attcccgcaa cgagagaaaa attgttgacc gatagagcta agagccagac tccgaaagct
     2161 tgaccaccaa tagctgaaat ataggtgatg aactgtgaaa aagaataagc ctccatcaga
     2221 tcatcttcag ctactttttc cttaataaga ggcatacgca ggccacctgc aaaatcactg
     2281 atgatatcac taatgacatt gatcaaacac aggctagaaa aggcaaagag actagcttgc
     2341 tgaacaacta gggctgctag aaaaaataga accgcctgaa acaaaccgct atagaccatc
     2401 catttgacct tgtccctcgt gtaatctgcc cgaatccctg caaaaactgt aaagagggtc
     2461 ggaagaatca tgacaatatt cgccatagca acagcaaaag atgcttgtga caaggtcgat
     2521 gcatagacga taaagaccag gttgaaaatc gaaacaccaa aagcattgaa gaagcgtgat
     2581 aaagtcaaaa ttctataagc tggatttcta aacaatagtt tcatagataa tctccctcct
     2641 tgttctaagt tctacatcaa gaatagcttt agtatagcac ttatgaattt gcgaaacgat
     2701 tcttttgaat atatgcaaca aacaaaatga gcaaaaaaac gatagaactt ttgtaatgac
     2761 ctgagagagg gcaacagttc tatcgcttca aattttttct tggtttgcag atattcaaga
     2821 atcgggagtt tttctatagt attcggcaga tttattacag ccaagcatct caaaaatacg
     2881 gacagcatcc tccatctttt tctggccttc cttgactcta ccttgcttgc tatcaaggag
     2941 accttctgcc cacagataaa caattcggaa ataggtctca ttttccttgt agaaatgctc
     3001 ttcgataaca cgtttaaaat aataggcatt ggtaaattct tcacactcaa tactagctaa
     3061 aaagccattc aatagtatag tatgaaaaag gtttcgattg ccagacattt ccattagaaa
     3121 atcggattta cgtaccattt ctcgtacata tctagtaaaa agagaaacag ataaaaatgg
     3181 agaactgact gaaaataaat tgagttcata gattccccag atctcggtag aaaacaaata
     3241 atcatgaagg acttttcctt cctctgctgt taagtctacc ctttcatcta tgctcttcat
     3301 ataagacttg ataataatgg catttagaat atgtttctgt ttgttgtgag aatgggcatg
     3361 cttttatact ccctgcgata taagtcctca agaggtgcta tattctttgg ttccaagaca
     3421 tctgtaattt cttttctcaa ctcagaatct gtatcatact ggaaacctct tgccagaaag
     3481 aggatctcct ccacactggc agatatattt tccagagcaa atagaaactt ttccaccgaa
     3541 agctcactct gacctgtttc aaaacgggac aacatagacg gcgaaaattg tcctccggtt
     3601 gcttgtctca gtgagatatt tcttgactct cgtaattgtc taaagacttt tccaatctgc
     3661 tccatagact tccccttgat tccgtatttt cttcatttta tcatattttt cagaaaattc
     3721 atcaaaaact tgccaaattg tcagaattat gagaaaatag aggatattta tcacgtggag
     3781 ggactgctat gagagacgat atcaaaatca atgaccgtgc tttggccttg caagaccaaa
     3841 ttatcgaaaa actagagaaa gtttttgata cagatgtgga attggatgtt tacaatctag
     3901 gtctgattta tgaaatcaat ctggatgaaa cggggctctg caagattgtc atgaccttca
     3961 ccgatactgc ctgtgattgc gccgaaagcc tgcctattga aatcgtggca ggtctgaaac
     4021 aaatcgaggg tatcaaagat atcaaggttg aagttacctg gtcgcctgct tggaaaatca
     4081 cacgaatcag tcgctatggc cgtattgccc ttggactacc acctcgttaa gcagaccaat
     4141 cacttttaaa gatgaaaaaa gcaagccgaa aatggcttgc tttttgatac tcaatgaaaa
     4201 tcaaagggca aactagaaaa ctagccgcag gttgctcaaa acactgtttt gaagttatgg
     4261 atagaactga cgaagtcagc tcaaaacact gttttgaggt tgtggataga actgacgaag
     4321 tcagctcaaa acactgtttt gaggttgtgg atagaactga cgaagtcagc ccaaaacact
     4381 gttttgaggt tgtggataga actgacgaag tcagtaacca tacctacggc aaggcgacgt
     4441 tgacgtgatt tgaagagatt ttcgagtatg agtttatttt ttacctgact tgtccatatt
     4501 ccagaagtct gtcacggctc cgcgtgaagc agatgatacg atgtgggcat atttaccgag
     4561 gacaccacgg ctgtaaagtg gtggcaaggt tgtttctgcc ttgcgttttt caagttcttc
     4621 ttcggatacg gccatagaaa tttctttggt atcttggtca accgtaacga tatcgccggt
     4681 acggagatag gcaattggtc caccatcctg agcttcagga gcgatatgtc caacaaccag
     4741 accataagta ccaccagaga aacgtccgtc cgtcaagagg gccaccttat ctccctgacc
     4801 tttaccaaca atcattgaag aaagtgatag catctcaggc ataccaggac cacctttagg
     4861 tccaacaaaa cgaacaacga ctacatcgcc atcaacgatt tcatctgtca gaacggcctg
     4921 aatcgcatct tcttctgagt caaagacctt agctggccca acgtgacgac gcactttaac
     4981 acctgatacc ttggcaactg caccgtcagg agcaaggttc ccgttcaaga tgataagcgg
     5041 accatccgca cgttttggat tttcaagtgg catgataact ttttggcctg gagtcaagtc
     5101 tgcaaagtca gccaagtttt cagctacagt cttaccagta catgtgatgc gatctccgtg
     5161 aaggaaacca tttgccaaca aatacttcat aaccgcaggg acaccaccga cttcgtagag
     5221 gtcttggaag acatactgac cagatggttt caagtcggcc aagtgaggca cacgttcttg
     5281 aatcgtattg aagtcctcaa gtgacaagtc aacatttgcg gcatgggcaa tggcgagcaa
     5341 gtgaagagtg gcgtttgtag aaccaccgag agccatcgtt acagtgatag catcttcaaa
     5401 ggcttcacga gtcaagatat ctgatggttt gagaccaagt tccaacatct taacaacagc
     5461 acgtcctgct gcttcgatat cttctttctt atcagctgat tcagctgggt gagaggatga
     5521 ccctggcaaa ctcatcccta gaacttcgat agcagttgcc atggtattag cagtatacat
     5581 accaccacaa ccaccagggc cagggcaggc attacattca agacgtttca cgtcctcagc
     5641 tgtcatgtca ccgtggttcc attttccgat accttcaaag acagaaacca agtcgatatc
     5701 tttaccatca agatttcccg gtgcaatagt tccaccatag gcgaaaatag ctgggatatc
     5761 catattagca atagcaatca tagatccagg catgttcttg tcacagccac cgatagcgac
     5821 gaaggcatcc acgttgtgac cactcatagc cgcctcgatg gagtccgcga tgatgtcacg
     5881 agatgttaga gagaaacgca taccaggcgt tcccatagcg atcccgtccg ctacggtaat
     5941 ggttccaaac tgtacaggcc aagcgcctgc agatttgaca ccttctttag ccagtttccc
     6001 gaaatcatgc aagtgaatgt tacatggtgt attttccgcc caagtcgaaa tcactcccac
     6061 aatcgatgtt tcaaagtcct tatctgtcat accagtcgca cgaagcatag cacggttagg
     6121 tgatttaacc atgctgtcat aaatgctact gcggtgacgt ttatctaatt cagtcatctt
     6181 atccctccca tttcagtttt tactattata gcacaatttt cgcatgaaga acagaataaa
     6241 attcttgaat tttcagaaaa ttctatacac atgtgaaata tttaaaatta aaaacaacaa
     6301 agcggattag tgcactttct gatgaccaga atatgctttt taatccgctt tctttaaata
     6361 acgtactgta atttttacag aaattctttc aaataagtgt atttaacatc tatcttgcat
     6421 tataaatttc tagaaccttc tcttttatat tcgattcact caaaccatac tcattaagaa
     6481 gataatccat tttccctact tgaccgaatc tttcttgaac acccatccga tgaatttttg
     6541 ttattccatc atcagagaat aattcacata aagcactgcc aattccacct atctgattgt
     6601 ggttttctac agtaaatata gtttttccac ttaacattgt ttttatctgt tctggtatcg
     6661 gtttgattct aaataaatct atcacaccta ctgaataacc taatttagac agttcatctg
     6721 caactcgaat acttggagca accattatgc cagaagcaac gattacaaga tcttcaccat
     6781 gccttaactc aatgtagcct ttagaaaaat cttctccacc ttgatacaca ggaactggag
     6841 cttttctaat tgttcgaata tattttagtc cttttaagtc taatgtctgg ttcaatattt
     6901 cacgaaattg gatatcatca gttgcttcga aaatgattga tttaggaatt aaacgtaaca
     6961 atccaatttc ttcaaatggc atatgtgttc caccattcat ctctgccgtt actcctgcat
     7021 ctgatccaat cacagtggca tccaattgtg cgtatccaag agaaataaat aattgatcaa
     7081 atactcttcg tgaagcaaaa ggaccaaatg tatgaagata aggtctaaac ccctgaatag
     7141 acaagcctgc tgcaaggccg accatttctg cttccataat cccaacattc acataacggt
     7201 ctccaaagtc cttttcaaga ttattagtag ccatcgaact tgacaaatcg gcttctaaga
     7261 ctactatatc agaatcactt tgattagcct ctaaaaggaa gtctctatat acatgccgta
     7321 attctttcgt acttctcatc attctgtttc ctccaattcc tgacttaatc tttctacaac
     7381 tgaagttaac atttgtttct cctctacagt agggcgaaga tgatgattgg atttcatttc
     7441 ttccagctct tgaacccctt gacctttaat agtatctaat acaatgcact taggtgatga
     7501 attatttgac tgttttaatt ggacaatccc ttcataaatt tctctaatat ctgaaccctt
     7561 gaccctaatg gattcaaatc caaatgctga aaatttttct acgaaatcac ctggattaca
     7621 aatatccttt gtaaaaccat ctaattgttt tttgttatca tcaacaaata caattaagtt
     7681 ggataactgt tgatgagaag caaactgtat agcctcccaa cattgtccct catttaactc
     7741 accatctcca acaatagcgt aagtataaaa gggactcttt cttattctct gaccatatgc
     7801 aagtccagtt gcaacactaa ttccttgtcc taaagagccc gttgtcatat ctatgcctgg
     7861 cgttagattt ctatcaggat gagacggtaa tttggttcca tttgtattta aagaatataa
     7921 gaattctttg tcaaagaaac cattcaaata gagtgtactg tatagagctg gtcctccgtg
     7981 accttttgat aatatgaaat aatctctatc tcgtgctgca aatatttctg gagtcattgg
     8041 cattatttca ccataaagca ccgctaaaac ttctacgata gacagacttc ctccgtaatg
     8101 tccgaatcca agatgattca atgttctaag agtatttaat cggatgttag tcgcaaattt
     8161 tcttaaccca tcttctctat ttttacttaa aatcatccct tattcctccg ttgcagatgg
     8221 ctttttaata aaggatactc caaacataac tgctagaata agaacaagac caatcacaat
     8281 gcctgcttgt gagccaaatt gatttaacat tcctaaaata attcctgata gaccaaaatc
     8341 tgcatctgag aaagttgatc cttggaaacc aagtcctccc aaaactggca ttaaaaagac
     8401 tggaagaaaa ctgattaaaa taccttgtaa aaatgctcca atagtggctc cacgaacacc
     8461 accagatgca ttcccaatga cacctgcagt cgctccacag aagaaatgag gcacaacacc
     8521 tggtaagata acaaccgttc ctgaagcaat cataattacc atacttacta aaccaccaac
     8581 aaaactagag ataaatccaa ttagaactgc attgggtgca taagtataaa caatcggaca
     8641 atccaaagca ggttttgaat taggtacaag acgctctgaa atacctttaa aggctggaac
     8701 aatttcgccc aaaataaggc gaacacctgc taaaataaca aatacccctg ctgcaaattg
     8761 acctgctaat tgtaaagcat aaactagacc acttgtacca ctactgattt ctttttctat
     8821 atattctgac cctgcaaaga tagctacaat aatgtaaata actgccatgg ataaagtaat
     8881 actaacagta ctatcacgta aaaaagctaa actctttgga aatttaatgt cctctgttga
     8941 ttttgatttg tcaccgataa ggctaccagt aaaaccactc aaccaatatc ccaaagaact
     9001 gaaatgacct aaagctacct tgtcatttcc agttaattga accatatatt tttgcacaaa
     9061 tgctggggaa atactcataa taataccgag tgctaatcct cctagtaaga tgagaggcaa
     9121 gctagtaaag ccagcaactg ataaaatgac cgcaatcata catgccatat atagagtgtg
     9181 gtgccctgtt aaaaaaatat atttaaatcg agtaaaacga gcgattaaga tattgaacac
     9241 catgcctgca aacataatca ttgcagtagc tgagccatat gttgttaaag ctacagctac
     9301 aattgcttca ttattcggca caacgccaga taaatgaaaa gcatgctcaa acatggtacc
     9361 aaatggattt aaagaatttt gtacaattcc tgcaccacca gatacaacta agaaaccaac
     9421 aaaggtctta attccacctt taataatatc aggtaatttc ttcttctgaa gaactaatcc
     9481 taagattgca attaaagcta ctaaaatagc tggtgtacta acaatatcca atatgaactt
     9541 catcatgacg ctagcctcct atataagtcc tttttcttca caaagtttag taattaattc
     9601 tcgtagttca tccatatcaa taatactatt taagatacga acatctccaa gatgactagc
     9661 tgaatcagct agatcacgac caacaatcca aatatcagct gcatttggat ctgctccacc
     9721 taaatcataa tgttcaactt ctacatccga aacattcaaa tcactcaata cagattcaat
     9781 attcatctgt accataaaac ttgaacctaa tcctgaacca caagctgtac caatttttaa
     9841 cattatctaa tcctcctgtt taattatcat tttaatgtca tcatagtttt ttgatgatat
     9901 taaagtttga acatgatttt tatctcttaa aattgttgtt aaatgtgaca aagcctttaa
     9961 atgactctca ttatcaatgg ctgcaataca aatcaacaat cttacctctt gttctggatt
    10021 atccaataaa taaatcggtt cttccaaaac taacattgac attcctattt cattcacacc
    10081 ttcatctggc cgagcgtgag gaattgctac tcccttccct aaattaataa aaggtccaaa
    10141 ctcttctact ttttgaatca ttgcctcagg gtagttctca gttatcttat cttgatccaa
    10201 aagcggttta gctgctaaac gaatcgcctc cttccatcct aatttttgcg aactaacctg
    10261 ataggtttct ttggtaataa gttgttctag cactggtaca atttcctttc tatcattttt
    10321 ttggtaaaga taattcttta acgccaatct taattccaat tcttgtgtaa taattccata
    10381 tcttttgaca atattcagga tttgttcaat ctcaaaatct ccatactcta aattcggaaa
    10441 atcttttaac actagttcta ctagttgtat tgcttgctct tcagtcatca taaccgaaac
    10501 tagataattt ggcttttctg tctccacctt tatggtagaa aaaaccatat catagtcact
    10561 actagctttc acctgtaaat catcaatctt tgaggttcct ataaactcaa tttgaggaaa
    10621 taatgctaat agattctctt ttaacatcaa tgaagaacta acaccattag gacaaatgat
    10681 tgctgcttta taccattttt gaggcaaagt atctgctttc tttaaataac ctccgaaatg
    10741 gataacaaaa tatgctgttt cactatcagg tatgggattg tcaatagcgt ccatcaaggg
    10801 catcaaagaa tctttgacta attcaaataa atcaggataa tgttctttaa catgcaatac
    10861 atattcattt gaactaggta ggccgaactt taatctatag taagccggta taaggtggcg
    10921 gcgaagattt tctctcaatc cttccctttg tttaaaatgt aacaaagaaa tatcttccat
    10981 tctacttata atagcctctg ttaattgatt aaagtaaacc ggagcaacat ctacttcacc
    11041 ttcaaagcaa cttgataata aaacggtgat atagcgataa tcatcctcag aaaacaccgt
    11101 atctataatt cccaaatcaa ccactgtatc caataaaata gtggttatat cttgaataac
    11161 aggagatact aatgtctctg aaagacatac tctttcaaca tccctttgat acctacacag
    11221 aatgaatact aaaccgaaaa ggtaaacttt taattgatta acaataggta ctagctgtag
    11281 cttctcataa taatctttaa ctacctgatc aatcaaatca taagttaatg aataccccca
    11341 actggataaa acataatcca aaccccaaat ccctatggag gattccagca actcactaac
    11401 catttgaaaa gctaagcggt gcttattcca ctctgaaccg tgtaaagtat aaccttttgc
    11461 tctactgtac cctagctcca aatcattatc taacataatc tttcttaatg attgaatatc
    11521 agataaggtt gtattcttac ttactttcaa aaagtcttgg taatgactat tcgatataaa
    11581 atctaatcgg caaaaagtgt aaagatagat taaagctaag cgagtcgact ttggtaaaac
    11641 caattcatcc gacttaataa tatctgtcaa agactgcttc gtacgatttg ataaactata
    11701 gcgaccttgc tttttatcca gcactatccc tttattagct agataaggca ctaaataatc
    11761 tattccttct ttgacttcct ttataggtaa gctcacctta acagataatt catataatga
    11821 tagctcacaa tgatccatca aagtcatcaa aataactagt gctctataat caaacatttc
    11881 tcttcctttc taactacaat tatagtgtaa aaattataag aataaaagag tatttgagca
    11941 caatataatc tcacctttta aaagaaatta ttgggctgaa agtttttagt gattattttt
    12001 caactttcta ttgactttat aaattaaagt gatatcattt agatatcaaa taacaggagg
    12061 tcattcttat gactgaaaaa ctaatcaatt caaaaccaaa tggtgtattc gcattgattc
    12121 tcattgagtt gacaatcgta cttggtatct ttatatttat aatgggggtt ggttcggaaa
    12181 acatttttgg aattattatc ggacctttac taatcgtaat tgcaggtcta gctcatgctg
    12241 gtttaaaagt tgtcaaacct caagaagctc tggttctgac actctttggt aactatacag
    12301 gtaccatcaa agaacctggc ttttactttg tcaatccctt cagcgtagca gtcaaccctg
    12361 caaaccacac tcgacttgga caaagtggtg atgttagcac aaaatctcct tttttaggag
    12421 ctaaatcatc aaatgacaat gatgtaaatc ttgaaattgg caagaaacag atttccctca
    12481 aagtcatgac cttgagcaat tctcgtcaaa aaatcaatga ttgcttagga aaccctgtag
    12541 aaatcggtat cgcggtaact tggagagttg tcgataccgc taaggcagtc ttcaacgttg
    12601 ataactacaa agaatatctt tcattgcaat gtgatagcgc cctccgtaat attgtccgca
    12661 tctatcctta cgatgtgtct cctaatgtgg atactacggg tgatgggcaa gcagatgaag
    12721 gtagtctccg tggctctagc gaaattgttg ctaaccgtat tcgtgaagaa atccaaagtc
    12781 gtgttgagga tgctggcttg gaaatccttg aagcacgtat cacttaccta gcttatgctc
    12841 cagaaattgc tgccgttatg cttcaacgcc aacaagcatc tgccattatt gatgcacgga
    12901 agatgattgt agatggtgct gtaggaatgg ttgaaatggc actagaacgg ctcaatgaag
    12961 gggaattggt agaacttgac gaagaacgaa aagctgccat ggtttcaaat ctccttgtcg
    13021 ttctttgtgg caatcatgat gcacaaccaa ttgtcaacac aggaagcctt tactaaagat
    13081 ggctgaagct aaaaaaaagc agatccccct tcgactctca acaaagttat acgctgcact
    13141 cgcatcatgg gcagaagacg actttcgttc agtcaatggg caaatcgaat atctcctcac
    13201 agagtgtgtc aagcaacgga agaaagacgg aaaatacgta tcagaaacca ttgacgaacc
    13261 atttgagatt gatatttaac accttctcct gtctgccgtg tcggatagga gaattttttc
    13321 atcttttttt gtcaagtaac tttactttac aaaaaaaatg tgttatccta gtatggttga
    13381 tgaaaatcag tagattgaat cgaatataaa tacctaaagg agaaatcaaa atggcagtac
    13441 ctgcacgtcg cacttcaaaa gcgaagaaaa acaaacgtcg tacacactac aaagtaacag
    13501 ctccatctgt aaactttgac gaaactactg gagattactc acgttctcac cgtgtatcac
    13561 ttaaaggata ctacaaagga cgtaaaatca ctaaagctgc atcagctgaa taatagaagg
    13621 gagataccat gcgcgtaaat attacacttg aacacaaaga atctggtgaa cgcttgtacc
    13681 ttacttctaa aaacaaacgt aacactccag accgccttca attgaagaaa tactcaccaa
    13741 aacttcgcaa acacgttgtg tttacagagg ttaaataagc attcaatacg aatcaataca
    13801 gaagaaaaac gcttgatcag cgttttttct ttttgttaat ttcactacat tgtaagggaa
    13861 atcattcgaa tgactacatt tttcactacc ttttttgaaa taagaatacg gattcggaca
    13921 attaaaagca agaggaactc aaatgtaagt tggggttcct tttttgtata tttttcaaat
    13981 aacgcctcta cactatttgt agcaaattca ccaactacag ttgtatctta gttaaaataa
    14041 gttagaatat gtaagtgagt accagatata ccaagacatc gtcaccatct aaggtatatt
    14101 caaaatacaa aagttgacca actagatttc tgaatatcct tatatatcca ttcttaaaat
    14161 tggtttaaat agcgtagtct tttaaactag ttttgagaat ccaaaaaatc ttcctacata
    14221 tgtaagaaga ttttttagtt cagaatgatt agatttagct aatggatacc tatcctaccc
    14281 actcaccgtt agcattgact cgatagccat cgggtgtagt cgtgtttact aataaatctc
    14341 ctgaattata ggtatagtac catttaccag aaactgtaac ccaaccagta gccattgaac
    14401 ctgattcatt taagta
//
LOCUS       DAVBBS010000053        14254 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVBBS010000053 DAVBBS010000000
VERSION     DAVBBS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14254
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.053"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            3..350
                     /locus_tag="VQU32_001653"
     CDS             3..350
                     /locus_tag="VQU32_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473077.1"
                     /translation="MLRLPYKKQKISHFTVSEDKDICRYIRQQLYYQNLFWMKEQAEA
                     YQKGENILTYGLKEWYPQIRPIVGKFFQIEQDLTSYYQHFYTYYQKNPQNDWQKLYPP
                     AFYQQYFLKNMVE"
     gene            369..2171
                     /gene="pepF"
                     /locus_tag="VQU32_001654"
     CDS             369..2171
                     /gene="pepF"
                     /locus_tag="VQU32_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET2473078.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEVLKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTFYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGIYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            2173..2886
                     /locus_tag="VQU32_001655"
     CDS             2173..2886
                     /locus_tag="VQU32_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET2473079.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELENFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            2953..3894
                     /gene="prsA"
                     /locus_tag="VQU32_001656"
     CDS             2953..3894
                     /gene="prsA"
                     /locus_tag="VQU32_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HET2473080.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(4011..4352)
                     /locus_tag="VQU32_001657"
                     /pseudo
     CDS             complement(4011..4352)
                     /locus_tag="VQU32_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            4765..4860
                     /locus_tag="VQU32_001658"
                     /pseudo
     CDS             4765..4860
                     /locus_tag="VQU32_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5113..5733)
                     /locus_tag="VQU32_001659"
     CDS             complement(5113..5733)
                     /locus_tag="VQU32_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2473081.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(5733..6215)
                     /locus_tag="VQU32_001660"
     CDS             complement(5733..6215)
                     /locus_tag="VQU32_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HET2473082.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(6215..7057)
                     /locus_tag="VQU32_001661"
     CDS             complement(6215..7057)
                     /locus_tag="VQU32_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HET2473083.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(7152..7952)
                     /locus_tag="VQU32_001662"
     CDS             complement(7152..7952)
                     /locus_tag="VQU32_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HET2473084.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            8096..9475
                     /gene="glmU"
                     /locus_tag="VQU32_001663"
     CDS             8096..9475
                     /gene="glmU"
                     /locus_tag="VQU32_001663"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET2473085.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            9485..10030
                     /locus_tag="VQU32_001664"
     CDS             9485..10030
                     /locus_tag="VQU32_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2473086.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            10042..10356
                     /locus_tag="VQU32_001665"
     CDS             10042..10356
                     /locus_tag="VQU32_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HET2473087.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            10373..11065
                     /locus_tag="VQU32_001666"
     CDS             10373..11065
                     /locus_tag="VQU32_001666"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HET2473088.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNTQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            11142..11633
                     /gene="rocS"
                     /locus_tag="VQU32_001667"
     CDS             11142..11633
                     /gene="rocS"
                     /locus_tag="VQU32_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HET2473089.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            11731..12321
                     /locus_tag="VQU32_001668"
     CDS             11731..12321
                     /locus_tag="VQU32_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET2473090.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGVLDLSQLFD"
     gene            12330..14093
                     /locus_tag="VQU32_001669"
     CDS             12330..14093
                     /locus_tag="VQU32_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET2473091.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         4485 a         2552 c         2969 g         4248 t
ORIGIN      
        1 aaatattgcg tcttccctat aagaaacaaa aaatatctca ttttacagtt tctgaggaca
       61 aggacatctg tcgctatatc cggcaacaac tttattatca aaatctcttt tggatgaaag
      121 aacaagcaga agcctatcaa aagggagaaa atatcctgac ttatggactg aaagaatggt
      181 atccacaaat tcgaccaata gtgggcaaat ttttccagat tgaacaagac ttgactagct
      241 attatcagca cttttatacc tattaccaaa aaaatcctca aaatgattgg caaaagcttt
      301 atccaccagc cttttatcag caatatttct tgaaaaatat ggtagaatag aaaggatgga
      361 ggaatctaat ggtattacaa agaaatgaaa taaatgaaaa agatacatgg gatctatcaa
      421 cgatctaccc aactgaccag gcttgggaag aagtcttaaa agatttaaca gaacaattgg
      481 agacagtagc ccagtatgaa ggccatctct tggatagtgc ggataaccta ctagaaatca
      541 ctgaattttc tcttgaaatg gaacgccaga tggagaagct ttacgtttat gctcatatga
      601 agaatgacca ggatacacgt gaagctaagt atcaagagta ctatgccaag gccatgacat
      661 tctacagcca gttagaccaa gccttttcat tctatgagcc tgaatttatg gagattagcg
      721 aaaagcagta tgctgacttt ttagaagctc aaccaaagct gcaggtttat caacactatt
      781 ttgacaagct cttgcaaggc aaggatcacg ttctctcaca acgtgaagaa gaattattgg
      841 ctggagctgg agaaatcttt ggttcagcaa gtgaaacctt cgctatcttg gacaatgcgg
      901 atattgtgtt cccttatgtc ctagacgatg atggtaaaga agttcagcta tctcatggga
      961 tttacacacg tttgatggag tctaaaaaac gtgaggttcg ccgtggtgcc tatcaagctc
     1021 tttatgcgac ttacgaacaa ttccaacaca cctatgccaa aaccttgcaa accaatgtta
     1081 aggtgcaaaa ttaccgtgct aaagttcgta actacaagag tgctcgtcat gcagccctcg
     1141 cagcgaattt tgttccagaa agtgtttatg acaatttggt agcagcagtt cgcaagcatt
     1201 tgccactctt acatcgctat cttgagcttc gttcaaaaat cttggggatt tcagatctca
     1261 agatgtacga tgtctacaca ccgctttcat ctgttgaata cagttttacc taccaagaag
     1321 ccttgaaaaa agcagaagat gctttggcag tcttgggtga ggattacttg agccgtgtta
     1381 aacgtgcctt cagcgagcgt tggattgatg tttacgaaaa tcaaggcaag cgttcaggtg
     1441 cctactctgg tggttcttac gataccaatg cctttatgct tctcaactgg caggacaatc
     1501 tggacaatct ctttactctt gttcatgaaa caggtcacag tatgcattca agctatactc
     1561 gtgaaactca gccttatgtt tacggagatt actctatctt tttggctgag attgcctcaa
     1621 ctaccaatga aaatatcttg acggagaaat tattggaaga agtggaagac gacgcaacac
     1681 gctttgctat tctcaataac ttcctagatg gtttccgtgg aacagttttc cgccaaactc
     1741 aatttgctga gtttgaacac gccattcacc aagcagatca aaatggggag gtcttgacaa
     1801 gcgatttcct aaataaactc tacgcagact tgaaccaaga gtattatggt ttgagtaagg
     1861 aagacaatcc tgaaatccaa tacgagtggg ctcgcattcc acacttctac tataactact
     1921 atgtatatca atattcaact ggctttgcgg ccgcctcagc cttggctgaa aaaattgtcc
     1981 atggtagtca agaagaccgt gaccgctata tcgactacct caaggcaggt aagtcggact
     2041 atccacttaa tgtcatgaga aaagctggtg ttgatatgga gaaggaagac tacctcaacg
     2101 atgcctttgc agtctttgaa cgccgtttaa atgagtttga agcccttgtt gaaaaattag
     2161 gattggcata aaatggttga atcgtatagt aagaatgcta accataacat gcgtcgtcct
     2221 gtcgtcaaag aagaaattgt agacttgatg cgtcagcgtc aaaagcaggt cacaggtttc
     2281 ttgaaagaat tggaaaactt tgcccgcaag gaaaatattc ctattattcc ccatgaaacg
     2341 gttgcttatt tccgttttct tatggaaacc atgcagccta aaaatattct ggaaattggg
     2401 acggctatcg gtttttcagc tctcttgatg gctgaacatg cgccaaatgc taagattaca
     2461 actattgatc gtaatccaga aatgattggt tttgccaagg aaaattttgc ccagtttgac
     2521 agtcgcaagc aaatcactct cctagaggga gatgcggtgg atgtcttatc tacactgaca
     2581 gagtcttatg atttcgtctt tatggattct gccaagtcta aatacatcgt ctttctgcca
     2641 gaaatcctca aacatttgga agttggtggt gtggttgtct tggatgatat ttttcaaggt
     2701 ggtgatgttg ccaaggatat tatggaagtc cgtcgtggtc agcgaaccat ttatcgaggc
     2761 cttcaaaaat tatttgatgc aaccttagac aatccagaac tcaccgcaac attagtgcct
     2821 ttaggagatg gtattctcat gcttcgtaaa aatgtagcag atgttcaact gtctgaaagc
     2881 gaatgatttt cagaaaaatt taagaaaaaa tagtaaaata gatagagtaa cacttatctc
     2941 aaaggagtag acatgaagaa aaaattattg gcaggtgcca tcacactatt atcagtagca
     3001 actttagcag cttgttcgaa agggtcagaa ggggcagacc ttatcagcat gaaaggggat
     3061 gtcatcacag aacatcaatt ttatgagcaa gtgaaaaaca acccttcagc ccaacaagtc
     3121 ttgttaaata tgaccatcca aaaagttttt gaaaaacaat atggctcaga gcttgatgat
     3181 aaagaggttg atgatactat tgccgaagaa aaaaaacaat atggcgaaaa ctaccaacgt
     3241 gtcttgtcac aagcaggtat gactcttgaa acacgtaaag ctcaaattcg tacaagtaaa
     3301 ttagttgagt tggcagttaa gaaggtagca gaagctgaat tgacagatga agcctataag
     3361 aaagcctttg atgagtacac tccagatgta acggctcaaa tcatccgtct taataatgaa
     3421 gataaggcca aagaagttct cgaaaaagcc aaggcagaag gtgctgattt tgctcaatta
     3481 gccaaagata attcaactga tgaaaaaaca aaagaaaatg gtggagaaat tacctttgat
     3541 tctgcttcaa cagaagtacc tgagcaagtc aaaaaagccg ctttcgcttt agatgtggat
     3601 ggtgtttctg atgtgattac agcaactggc acacaagcct acagtagcca atattacatt
     3661 gtaaaactca ctaagaaaac agaaaaatca tctaatattg atgactacaa agaaaaatta
     3721 aaaactgtta tcttgactca aaaacaaaat gattcaacat ttgttcaaag cattatcgga
     3781 aaagaattgc aagcagccaa tatcaaggtt aaggaccaag ccttccaaaa tatctttacc
     3841 caatatatcg gtggtggaga ttcaagctca agcagtagta catcaaacga atagtccaaa
     3901 tcaatgagtc agggaaaaaa actcgacttc aggaaaaaat gaagcaaaca ttcccacaat
     3961 aaaacgcata gtacaaggtt tgtactgacc ccaaaagtta gacaattaat ttatctgaag
     4021 gatttagttc tgtattgcac agggctaagt ccttttagtt ttaccttaat tctcttattg
     4081 ttgtaataat caatatagtc tataatggct tgttccaatt gattaagtga tttaaatgtt
     4141 ttctcatagc cataaaacat ttcggatttt aaaatgccaa agaaagattc catcctaccg
     4201 ttgtcttggc tgtttccttt gcgtgacata gatgcttgaa ttcccttact ctctaggaag
     4261 cgatgataag aatcgtgttg gtattgccag ccttggtcac tatggagaat cgtattctcg
     4321 tagtgcttct ctttgaatgc ctgttccaac attaacgatc aatcaattta atcatgtacc
     4381 taatattaga attgtttatc ccaaatttat ttgaaagctt ctctaagcta tatccttgtt
     4441 ttctaagttc atagatctga actttatcat cataagttaa tttcataata aaaacacccc
     4501 aaaagttaga ttttttctgt ctaacttttg gggtgtagtt catgtacacc tgatatgatg
     4561 cgttttataa ttttaaagac tttttgacca gcctcatttt tttaacttga tactcagtga
     4621 aaagcaaaga ttaaactagg aagctagctg taggctgctc aaagaacagc tttgaggttg
     4681 cagataaaac ttgtgaggtc accaacatat ataatgtgaa gctgacgtga cttgaataga
     4741 ttttagaagg gtatgagtct ggaagtttta atggataatg caagattcca tagaatgggt
     4801 aagctagagt tcttatgtga agagtttggg cataaacttt taccttttcc tccctactca
     4861 tcttagtata gaaaagtgaa tctaaaatag tacataactg cttctaaaac attcttataa
     4921 attgatttaa attctcaaat catattattc agttcttatt tcattttgct ctacaatcct
     4981 gttgagaaga cacgtgttca tatcaaaaag gtattggcaa gttgcaatac ctttttacaa
     5041 ggttcttttg tcttattttt gtttcaactg actatatctc ctatggttct agttcagaag
     5101 gctaggctat aattatgatt gataagaagt atcattccaa gtattgagag tgaatgtttc
     5161 aaaatcatgg gtttctataa tggtcaggct ggcatttgct agaccgccat ctttacgaag
     5221 aagtggttct ttatagccta ggagagtacg aagactggca gtaagattgg cgccgtgtcc
     5281 gacaattaga atacgctcag ctggactatc ttttaatgat ttgataaatt ggatggtccg
     5341 ttgagttgta ctatagaggg attcggctcc gaacattcga gtgtcaaatt gagcaagatt
     5401 tgaacgaaaa gcctggattt gttgcgggta aatagcttcc aaggttgcaa ttttcaaacc
     5461 ttctaacttc ccaagttgcc attcacggag attaggaacg atttctaaag aacagggggt
     5521 atagagttga ctttggataa tctcagcaga tttgaccgct cgaggtaaat cacttgaata
     5581 aatctgatca aaaggaattt ccttgagata ctggccaagt cgttttaggg tttcaatgga
     5641 ttcaggaaga agaggagaat caccgctagc accttgaaaa cgaccttctt ggttccagag
     5701 ggtacgaccg tggcggacaa agtagagttt cattacttga tgtcctccaa aatatctaca
     5761 aagtctgcct ttacaaagct agccaagtct tgtggcgcga cgataatgct gtgtccgact
     5821 tcgcctgcag agacaatcat tcgatccaaa tctagagcaa ttttatcgat aaaaatggga
     5881 taattgtgtt tctgacgaat tccgacagga ttattggctc catgaatgta accagttgtt
     5941 ttttctaagt ccttttgtgg aatcatgctc acttttttat tgccagaaat tttagctagt
     6001 ttcttttcag acaagtgctg agtgataggg acaattccga taatcggtcc ggtcttgtct
     6061 cccaaaagcg ccaaggtttt gaaaatctga tctcgttcat aaccttgagg aagctctcct
     6121 tctagggcat tgatttgaat cccctgatga gggatagctg ctttagatag gatttgttcc
     6181 accaatgttt ttttgatttt aacttttttt gccattattt atatttatcc tccaattgac
     6241 tcatccaaat accaagccag attcccagcg caaagaagaa ggcgatgatg acataaccga
     6301 caagtgaaag tcctgtgtat tggatacttt cagcgtttcc tgcatttgga attaagatca
     6361 aaagggtact tgataggacg ataccgatga tgaaatgata gacgcgtgag tggtagttgt
     6421 ttaaggcata atccatcaat tttgaaaaaa cgatgagagt tgcacctgca ccaattccaa
     6481 tcggaaagaa ggttcccaag agatcaaaag ttttaaaacc agtcaacata ggagcataga
     6541 gtcccaaaat caaaagtaag ttcgatgggc tgaggccagg aaccaagacg ccaagggcca
     6601 atagtgcgcc tgctaggacg aagttaagaa agctggcgct taaggttcca acgacaaaat
     6661 ttaaggcata gagtcctaat ccagaaatga taaaggttgt ccataaccaa gctaaatcaa
     6721 tcttgtctcg gtcagattct cgagttgatt ctttgaggag gctaggaact gtaccgataa
     6781 tagctcccgc aaagctccat aatacaaaga cctgataatt ttcaagcagg tattcaatcg
     6841 ggtaggaaaa taagccgatt cccagaagca taccgatggc aactggaata aagtacaaaa
     6901 cattttcttt aaggtcttta aagggatggg ccagaaagcc aatcattcgt tcatagattc
     6961 ctaagattgc tgctagaacc cctccggaaa ttcccggtag gataaatcca agagcaatta
     7021 caatcccttt aataacgcgt gctaaccatg agagcatata tttcctccaa ctatttctat
     7081 ttcaaaaatt ccttactata ttgtatcaca atcagacaca aaaagaaaaa gcaaatgata
     7141 aacaaatgct tttaaggttt taaaaaaagt tttcgaaagg tttcttcttt attttttaag
     7201 ggagagataa cgttgatatc taaatcgtgg tcaaagccgg caatttttcc tttagatgtg
     7261 tattggtgaa tatcataatc taaatcagtt ttaggactgc tctccaaaaa tcctgagtct
     7321 gagccgtagg acggaatcca aacagaggta aacttgcctg tatcaatact gtgttcttcc
     7381 atgaagtaga caccaacgta gatgccgatg tttttagcac ctagtgatgc tagttttgct
     7441 cgaaagtttt cgacaccttc gttcatatta gacatggttt tgtcttccac gtcaagccaa
     7501 tagtaactag ggctgtaagg agaggcagca ttgtagaaaa cttcggcagc cttttccatt
     7561 tcttggacac tttttccagc tacataagcg tagacagcaa ctgggacatt ccgcttttga
     7621 agttcagtga tatgactctt ataggccttg tctattccat tgataaatga agcatcattt
     7681 tcttttgttg tttgagcacc actgtgaaca cgaacaatag cacctgaaat attttgtgag
     7741 agggcatcgt agttgatttc ctcaggacgc tgccagccag agaggtcaat aatcggtttg
     7801 tctaagtgtt tcaaagcctg tgcttcaatc tgtgctatat tggattttgt tttaaacgat
     7861 tggctgtcat taagtgggcg attgatgatt aaaatgaaca tcataatccc aaaaaaacta
     7921 aataaaataa gtggatgaat ttgttttctc atatcttata agtctaccct aaaaatcaaa
     7981 aaaaatcaaa aaaatgggtt aaggaagaga ctttagagca ttttttcatt caagagtgcg
     8041 gaatgatttg aaatatggta taataaaagg gaatttctac agaaaagaga agattatgtc
     8101 aaattttgcc attattttag cagcgggtaa agggactcgc atgaaatctg atttgccaaa
     8161 agttttgcac aaggttgcgg gtatttctat gttggaacat gttttccgta gtgtgggagc
     8221 tatccaacct gaaaagacag taacagttgt aggacacaag gcagaattgg ttgaggaggt
     8281 cttggctgga cagacagaat ttgtgactca atctgaacag ttgggaactg gtcatgcagt
     8341 tatgatgaca gaacctatct tagaaggttt gtcaggacac accttggtca ttgcaggaga
     8401 tactccttta atcactggtg aaagcttgaa aaacttgatt gatttccata tcaatcataa
     8461 aaatgtggcc actatcttga ctgctgaaac ggataatcct tttggctatg gacgaattgt
     8521 tcgtaatgac aatgctgagg ttcttcgtat tgttgagcag aaggatgcta cagattttga
     8581 aaagcaaatc aaggaaatca acactggaac atacgtcttt gacaacgagc gtttgtttga
     8641 ggctttgaaa aatatcaata ccaataacgc tcaaggcgaa tactatatta cagacgtcat
     8701 tggtattttc cgtgaaactg gtgaaaaagt tggcgcttat actttgaaag attttgatga
     8761 aagtcttggg gtaaatgacc gtgtggcgct tgcgacagct gagtcagtta tgcgtcgtcg
     8821 catcaatcat aaacacatgg tcaacggtgt tagctttgtc aatccagaag caacttatat
     8881 cgatattgat gttgagattg ctccggaagt tcaaatcgaa gccaatgtta ccttgaaagg
     8941 gcaaacgaaa attggtgctg agactgtttt gacaaacggt acttatgtag tggacagcac
     9001 tatcggagca ggagcggtca ttaccaattc tatgattgag gaaagtagtg ttgcagacgg
     9061 tgtgacagtc ggtccttatg ctcacattcg tccaaattca agtctgggtg cccaagttca
     9121 tattggtaac tttgttgagg tgaaaggatc ttcaatcggt gagaatacca aggctggtca
     9181 tttgacttat atcggaaact gtgaagtggg aagcaacgtt aatttcggtg ctggaactat
     9241 tacagtcaac tatgacggca aaaacaaata caagacagtc attggagaca atgtctttgt
     9301 tggttcaaat tcaaccatta ttgcaccagt agaacttggt gacaattccc tcgttggtgc
     9361 tggttcaact attactaaag acgtgccagc agatgctatt gctattggtc gcggtcgtca
     9421 gatcaataaa gacgaatatg caacacgtct tcctcatcat cctaagaacc agtaggagcc
     9481 tatcatggag tttgaagaaa aaacgcttag ccgaaaagaa atctatcaag gaccaatatt
     9541 taaactggtc caagatcagg ttgaattacc agaaggcaag ggaactgccc gacgggattt
     9601 gattttccac aatggggctg tctgtgtttt agcagtaacg gatgaacaaa aacttatctt
     9661 ggtcaagcag taccgcaaag ctatcgaggc tgtctcttac gaaattccag ccggaaaatt
     9721 ggaagtagga gaaaacacag cccctgtggc agctgccctt cgtgaattag aggaagaaac
     9781 agcctataca gggaaattag aactcttgta cgatttttat tcagctattg gcttttgtaa
     9841 tgagaagtta aaactatatt tagcaagcga tttgacaaaa gtggaaaatc cgcgtccgca
     9901 ggatgaggat gaaaccttgg aagtccttga agtgagctta gaagaagcga aagaattaat
     9961 ccaatcaggt catatctgtg atgccaagac aattatggct gttcagtatt gggagttgca
    10021 gaaaaaatag aggaggtcag tatgggtaaa tctttattaa cggatgaaat gattgaaaga
    10081 gctaatagag gcgaaaaaat ttcaggtcct cctttgctag atgataatga ggaaactaag
    10141 attttaccaa cctcttcttc ccgttttggt tatgccaatc ctaaggatca tggttttagc
    10201 caggaaacct tgaagattca ggtcgaacca tctattcata aaagccgtcg tattgaaaat
    10261 accaagagaa atgtcttcaa ttctaagttg aataaaatct tatttgcggt catctttctc
    10321 ttgattttgc ttgttttagc aatgaaactt ttgtaataga aaaggaattg aaatgaaaat
    10381 aggaattatt gctgctatgc cagaagaact ggcttatctg gtccagcatt tagataatac
    10441 ccaggagcaa gttgttttgg ggaataccta tcatacagga accattgctt ctcatgaagt
    10501 cgttcttgta gaaagtggaa ttggtaaggt catgtctgct atgagtgtgg cgattttggc
    10561 tgatcatttc caggtggatg cccttattaa tacgggttca gctggggcag tagcagaagg
    10621 tatcgctgtt ggggatgtcg tgattgctga caaattagcc tatcatgacg tggatgtcac
    10681 agcttttggc tatgcttatg gacaaatggc gcaacaaccg ctttatttcg aatcagacaa
    10741 aacctttgtt gctcaaatcc aagagagttt atctcaattg gaccaaaact ggcatcttgg
    10801 tttgattgct acaggagata gttttgttgc aggaaatgac aagatagaag cgattaagtc
    10861 ccatttccca gaagttttag ccgtggagat ggagggggca gctattgctc aagcagcgca
    10921 tgccctcaat ctcccagtct tagtcatcag agctatgagt gacaatgcca accatgaagc
    10981 aaacatcttt tttgatgagt ttattatcga agctggacgt cgctctgccc aagtcttgtt
    11041 gacctttttg aaggctttag attaagcgga aatttgacag tttttctagc ttatgataag
    11101 atttaagtaa agaaaagcta gaaaacgttt cagaggatat tatgagtatt gaaatgaccg
    11161 tcagtgagat tgcagaggtc ttaggattat ctcgccaagc aatcaataac cgtgtcaaag
    11221 aattaccaga agaagacaca gataaaaatg acaaaggggt aacagttgtt accagaagtg
    11281 gcttgattaa gctagaagaa atctataaaa aaacgatttt tgaagatgag cctgtcagtg
    11341 aagatgtcaa acaacgtgaa ctgatggaga ttctagtgga tgagaagaat gcagaaattc
    11401 ttcgtcttta tgaacaatta aaagccaagg atcgtcagtt atcagaaaaa gacgagcaga
    11461 tgcgtatcaa agaccgtcag attgctgaga aagacaaaca attagatcag caacaacaat
    11521 tgacccttca agccatgaag gatcaagaaa atcttaagct agagctggac caagcaaaag
    11581 aagaagtcca atccactaag aaaggctttt ttgctcgttt atttggagga taatcaagga
    11641 gctgtttagg ttaaatgcta acctgatggc ttcttttgtt tgtgaatagt atagttgatt
    11701 agaacagaga cagtagagga gaggtacaga atggaacgat tagaagatta cattgctttt
    11761 gacttagaat ttaatcagca cgaaggacaa acacacttga ttcaagtatc ggcagtgcgt
    11821 tttagagatg gtcgggaaat cgatgcctat gattcctatg ttcataccag cgtccctcta
    11881 aaaagcttta tcaatggttt gaccgggatt acagctgaga ccttaaaaga tgctcctcca
    11941 gttgagaaag tcttagaaga attccaagag tttgtgggtt ctttgctgat gataggctac
    12001 aatgctgcta aaagtgattt acctatttta gcagagcatg gtttagacta tagccaacag
    12061 tacaaggtgg atttgtatga tgaagctttt gaacgtcgga gttcagattt acatggtatt
    12121 gccaatctca aattgcaaac tgttgcctca tttcttggat ttcaaggtca gtctcataat
    12181 agtttagagg atgctcggat gacggcgcgt gtttacgaat ccttcttaga gtcggatcaa
    12241 gctagggaat tgttagagac agaaagtagc ttatcaaaca atccttttgg tgtcttggac
    12301 ttgtctcaat tatttgacta ggagtgccta tgaaacctga aaaacctaaa aaattgggtc
    12361 ttaaacagat ttacctcaat cttgataaaa tcctctttct atttttcttg atttttatga
    12421 tagtggagta tttgtgggta ccattcaatt catggttggc tagcttctta ttagatcaga
    12481 caggttatct ttttatttcc tataacaatg tcttagccat ccttactcat tctccattga
    12541 ttagtctagc ttttttagcc ttgattgtaa ttaaccttct ggttgcttat tttcaaatag
    12601 gactcctctt tattggtgct cgtcatctcc tctataatga aaagcgaagg ctaattgagt
    12661 atatccgcaa ggttttccat gaaagttttg gatttatgaa aaagctaact tgcgctaaag
    12721 ccttgtttat ctttttctat atagctatgc tttttccttt tatacggaaa atgctgaaga
    12781 tttattatct taataaaatc gtcattccag agtttatcca agcttattta gaagataaat
    12841 attggatgtg gtggctgagc atcctacttc tatctcttat ctttctctat gtctctgtca
    12901 gattgatgtt tgcccttccg aagattgtat atgatcagct gactgttcga gaagcagtga
    12961 tgtttagctt ggagaaaacc aagaagagag ttacttttta tgcttggaat ttattttaca
    13021 tcttgctcaa agcacattta ttattttatc ttcctttgat tcctttatta ttggctcaaa
    13081 ctttggtaga tgatatcact cagagagagt ctctgattct tggtattttg aattttgtcg
    13141 tgattaaaaa tctctattat atggctctga cttatttttt ggttaaattt gtttcatttt
    13201 tgacaggaaa ggagctggat atgcttccta ggagagaaaa agatcacatc gtgcgatggg
    13261 gtgtcatgac ttgtgccagt cttttctttg ccttagaagg ttatatttat ctggaggctc
    13321 ccatggttca tctacctcaa cttatttctc accgaggggt ttccaatgca aatgggattc
    13381 aaaatacagt agagtccttg gaaactacag cacaactcaa accagacttg gtggagacgg
    13441 acgtgcagga aacaaaagat gggcagtttg tcatgatgca tgatgctaac ttgaaaaatc
    13501 tagcaggtat caataaaagt cctcaagact taaacttgga ggagcttaaa gggattgata
    13561 tttttgaaaa tggctaccag actaaaattt caagctttga agattatctc agtcgagcca
    13621 acgaacttgg tcaaaaatta ctaattgaaa ttaaaaccag taaaaaagat agtccagaca
    13681 tgatgaaccg ctttttagcc cgttatgctg caaagctcaa gatttatgga catcaaatcc
    13741 agtctttaga ctaccatgtt gtcgaaaaag taagacagta tgatgccgaa ctgccagttt
    13801 atttcatcat gccctacaat tctgtctttc ctaaaacaag agcgacagga tatactatgg
    13861 agtactcaac cttggatgaa tattttgtaa gcaaactatg gacaacggat cagaaacttt
    13921 atgtctggac tgtcaatgat tcagaagcta tcagcaaatc tcttcactta ggagtagatg
    13981 gagtgattac agatgatttg gaaaaagttc agcgggaaat agaagtagcc caagaagatc
    14041 cagaatatac ggatttgctc ttgaaaaaag cattggaatt ctttgaattt tagtaataaa
    14101 aaagaggaac ggttgttcct cccatgatag gtaagtattc ttgtgaatgc ttgctttttt
    14161 gtggttgcaa tccgtttacg agaattgttt gtattgttct tgtttcaatt tactttatag
    14221 tgctttgaag ttggaatgct acactataac ttct
//
LOCUS       DAVBBS010000054        12573 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVBBS010000054 DAVBBS010000000
VERSION     DAVBBS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12573
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.054"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(251..433)
                     /locus_tag="VQU32_001670"
     CDS             complement(251..433)
                     /locus_tag="VQU32_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473092.1"
                     /translation="MAVIFKNVLIEEKTEEEKQHILSILNGEYDLSDDVSCRLRRLNE
                     ESKRLEVFVKETNSLV"
     gene            complement(649..1881)
                     /locus_tag="VQU32_001671"
     CDS             complement(649..1881)
                     /locus_tag="VQU32_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET2473093.1"
                     /translation="MKIAVAGTGYVGLSIAILLAQHHQVVAVDIIPEKVELINKKKSP
                     IQDDYIEKYLAEKELNLVATLNAEEAYENADFIVIAAPTNYDPKKNFFDTSSVEAVIE
                     AVMKYNPDAIIIIKSTVPVGYTKSVRRKFLTDNIMFSPEFLRESKALYDNLYPSRIII
                     GTDKDDKDLVKSAEIFVKMLQEGAVKENIETLYMDFTEAEAVKLFANTYLALRVSYFN
                     ELDTYSEMKGLDTQAIIDGVCLDPRIGSHYNNPSFGYGGYCLPKDTKQLLANYSDVPQ
                     EMITAIVQSNRTRKDYIADRILDLAGSYVGNDFYKPDKEKEIVVGIYRLTMKSNSDNF
                     RQSSIQGIMKRLKAKGVTIIIYEPTLESGTTFYGSQVVNDFEIFKKSSQVIVANRFDE
                     TLEDVRGKVYTRDIFKRD"
     gene            complement(1910..2980)
                     /gene="gla"
                     /locus_tag="VQU32_001672"
     CDS             complement(1910..2980)
                     /gene="gla"
                     /locus_tag="VQU32_001672"
                     /EC_number="5.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate 4-epimerase Gla"
                     /protein_id="HET2473094.1"
                     /translation="MRGKTILITGVAGFIGSNLVRKLLELEGTMTIIGLDNLNDYYDI
                     ALKDYRLLQLNKLIQDYPEKNWIFIKGDIADKMLINKIFKSYHPQIVVNLAAQAGVRY
                     SITNPDVYIESNIVGFHNVLEACRHSYEIYDGGVEHLVYASSSSVYGGNKKIPFATED
                     KVDNPISLYAATKKTNELQAYAYSKIYNIPSTGLRFFTVYGPAGRPDMAYFGFTDKLR
                     SGSDIEIYNHGNCKRDFTYIDDIVEGIVRVMQSAPKKLVGSDNLPLAPYKVYNIGNSK
                     PENLLDFVDVLQQELIKAGVLPENYDFDSHKKLVPMQPGDVPVTYADTSDLERDFGFK
                     PKTSLREGLRKFAEWYKDYYNV"
     gene            complement(3048..4217)
                     /locus_tag="VQU32_001673"
     CDS             complement(3048..4217)
                     /locus_tag="VQU32_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET2473095.1"
                     /translation="MRLSKKYWFALTSKFLLILLTLLSSIIINRGLGVEKKGDYAYVM
                     NLVEIFYILGGVGLGQAYSTFKRNHSNEYQNKFVFLTFIQGLLFGIIGLIIVLADVPI
                     DYGGVIIVLSSLSIMRTNLTMIAVIENSIKRNKIVLGVSVVYSVMLVLLLLFHLVSIE
                     MVFLVYAINEFLRIILFLYYYNMFPVFEHISIKEFFTIYKFGFLSMILTLLITFNYSI
                     DIIMLKQMSSSYNVGIYSVAVTFSNMFLMVPDSFKEILFGDSAKRTFEKNKAINAIKV
                     SFLFMILLIIGFLSFGEFAITLLYGSSYIKAFPITLVIFLGSLSMIFFKILQPIYISQ
                     GKQSVAIKILSVSALINISLNWYLIPNYQITGAAIASAISYTVCGLFFIIDYLRN"
     gene            complement(4403..5707)
                     /locus_tag="VQU32_001674"
     CDS             complement(4403..5707)
                     /locus_tag="VQU32_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HET2473096.1"
                     /translation="MKFSLQSNDIKNKIILLVGVVFSGTLILCSENNDVTIPLFSMIF
                     LSFMAMIIVKFNIFHPYVWYSSFFTIYSISYPLLYRMEYINYGYTKDLIFMQWLALAT
                     LLLTLPSKAIVLSERKINLNNNNYLLFLNNMNTFITTVAIIYLLGSGFRNKGEIYSGA
                     NIVIMLVFSLVYFMILIHTYQLFIQLSTKKWRLLNCNTNTAIVICLFSIVTGERDYMF
                     TLFILDVIILFYFHKIKRFYLVMLVPLAALLIPLSAVFKYTLLSGQVSSINTNNIWFD
                     LLDGEFVSASRNLQILILHNMGNYFEGRSFFNDIVRIFYNTGYSNQTWFMDTFFPNVH
                     STKYGFTLVGEGYVNGGYFGIVMIFMLTGFLMRFLYINAQRNIYGMLIYLYMIPIFIY
                     STRADFANILSPLLKYAILGTLVIVFINRMVLISPLDEMGGE"
     gene            complement(5824..6978)
                     /locus_tag="VQU32_001675"
     CDS             complement(5824..6978)
                     /locus_tag="VQU32_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2473097.1"
                     /translation="MKVFIATEFRCTIYKNEIYLKPKAYFIYERYAKAFGEIVLCSRY
                     EKVESYPEGYHKANFINKFIPIEGLHQVLFGQNKNKIIEGMIDSDLIVVRIPSIIGSK
                     TADYALKIGKPYLTEIMGDAWDSYWYHSLKGKLLAPYIYAKTKSIVKNANYCIYVTEK
                     YLQDRYPNIKSNIVASNVNITSVENRSLKSRLYKLKKFNPQKISIMTTASVNVRAKGH
                     RFVLEAMKRLEIQGILLDYYLAGDGDQSFLKKKAEELGVANRIHFLGELTTSQIYEYL
                     DKVDLYIQPSLQEGLPRAVIEAMSRACPCIGSNKAGIPELLGPHCLFTPSSSQAIAGS
                     IIDFISMDKEKMVIKQFEFSKKFLSSVLNKRRSDYFAQIRRELNNSDGLD"
     gene            complement(6975..7526)
                     /locus_tag="VQU32_001676"
     CDS             complement(6975..7526)
                     /locus_tag="VQU32_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET2473098.1"
                     /translation="MNRIIKNIVGLLRANICLILLKVCRNKDLTFHYFLKFGKNVTVE
                     QESNAKLILGKKIRVNAGGVLKVRKGAKLKISDDVFLSNNCMIACRKYIDIKSGVKCG
                     PGVLIYDHDYDVSVPGGLKAKKFKTAPVMIGENVWIGANSIVLKGVSIGENSVVAAGS
                     VVTKDIPADTIFIQKRLSREMKL"
     gene            complement(7519..8655)
                     /locus_tag="VQU32_001677"
     CDS             complement(7519..8655)
                     /locus_tag="VQU32_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2473099.1"
                     /translation="MNLQKKKIMLITNHDDDIYCFRKEVIESLVEYGYEVLVSCPYGD
                     KIELMKSIQFEYEDITIDRRGINPLKDIRLFLSYIRLLRKYRPDVVLTYTVKPNVYAS
                     LAATILGIKYINNVTGLGSVLTMGSCVRSLVLLLFRISFRKSNCVFFQNEYNMKMLKT
                     LGLILGDSKLIPGSGVNIEKYPVQQYPNGGNGIQGETIVFNFIGRILKEKGIDTYLAA
                     AQIIKSRYPKTEFNIIGFIEPTESNYELKICDLEKKGIVYYLGQQKDVIPHITRSHAI
                     IHPSVYGEGMSNVLLENASSGRVLITTDNPGCKEIVKDRETGYIFQGGNVEELVSILE
                     VFLGLENEKRKEMGLQGRMYIEQYFSRQIIIDAYLDKIKGALDE"
     gene            complement(8796..9461)
                     /gene="cpsD"
                     /locus_tag="VQU32_001678"
     CDS             complement(8796..9461)
                     /gene="cpsD"
                     /locus_tag="VQU32_001678"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HET2473100.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNTLCTNIQLSGDKLKVISVTSVN
                     PGEGKTTTSVNIAMSFARAGYKTLLFDGDIRNSVMSGFFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTPPIGIVI
                     DAAIITQKCDASILITATGEANKRDVQKAKQQLEQTGELFLGVVLNKLDISVDKYGVY
                     GRK"
     gene            complement(9471..10163)
                     /gene="cpsC"
                     /locus_tag="VQU32_001679"
     CDS             complement(9471..10163)
                     /gene="cpsC"
                     /locus_tag="VQU32_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HET2473101.1"
                     /translation="MKEQNTLEIDVLQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYLVNRNQGEKPGLTNQDLQAGAYLVKDYREIVLSQDVLEEVISDLKLD
                     LMPKGLANKIKVTVPVDTRIVSVSVSDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPAISPSSPNIKRNTLIGFLAGVIGTSVIVLLLELLDTRVKRPEDIENALQMT
                     LLGVVPNLGKLK"
     gene            complement(10172..10903)
                     /locus_tag="VQU32_001680"
     CDS             complement(10172..10903)
                     /locus_tag="VQU32_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase CpsB"
                     /protein_id="HET2473102.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYRQIHTGLSNILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QINSYHVSKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKAKELFVDNPRKIIMDQLI"
     gene            complement(10905..12350)
                     /locus_tag="VQU32_001681"
     CDS             complement(10905..12350)
                     /locus_tag="VQU32_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET2473103.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLICFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLLFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSKIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADS
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLTDGDRDRGRNQQKVIVAIIQKLTST
                     EVLKNYSSILQGLQDSLQTNIPIETMIDLVNTQLESGGNYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLTVVKAAIQDVMEGR"
BASE COUNT         4237 a         2433 c         1656 g         4247 t
ORIGIN      
        1 aattcactta cttcatatta catttagcat gaaatgaaat aagcgaattc tactttgttt
       61 gcatacaatt aaaaatcaat tcctaaggat gttttaaata actgtgtaaa aggcataatt
      121 tcaccatttc ttagtttttc aatgctaaat tacatagtaa ctggagagct attagaaatc
      181 tctaacttcc tcatagttaa aaaatgacaa tgagatactt attgatttca attcattaac
      241 agaatacgaa ctagacaagt gagttcgtct ccttaacaaa cacttccaag cgtttacttt
      301 cctcattcag tcgtcgtaat ctacaactta catcatcaga taagtcatac tcaccattta
      361 gaatagatag aatatgttgt ttttcttctt cagtcttctc ttcaataagc acatttttaa
      421 aaattactgc cattatcagc acctcacttc gaaggaatat atgcaactat atcctattaa
      481 gtcaatctct actgacaaaa gaagtatatt tacttcacta agtgcgagcg agaatataga
      541 aatatcttta ttgttttaga tgagttgtcg ctatcctcca gctagttttt aactattcta
      601 tcattcaaat gatatagatt attagggact agaaatatac aaattgattt aatctctctt
      661 aaaaatatcc ctagtataaa ctttacccct cacatcttct aaagtctcat caaaacgatt
      721 tgcaacaatc acttgactag atttcttaaa tatttcaaaa tcattaacta cttgactacc
      781 atagaaagta gtaccacttt caagagttgg ttcgtaaata ataatagtaa caccttttgc
      841 tttcaaacgc ttcataatac cttgaataga agattgtcta aaattatctg aattactttt
      901 cattgttaaa cgataaattc ctacaacaat ctctttttct ttatctggtt tatagaaatc
      961 attgcctaca taagatccag caagatctaa aatacgatct gcaatataat cttttcttgt
     1021 ccgattactt tgtactatag ctgttatcat ttcttgtgga acgtcagaat aatttgcaag
     1081 aagttgcttg gtatctttag gaagacagta acccccataa ccaaaagaag gattattgta
     1141 gtgagaacct attctcggat ctaaacaaac tccatcaata atcgcttgag tatctagtcc
     1201 tttcatctca gaataagtat ctaactcgtt aaaataggaa acccggaggg caagataggt
     1261 attagcaaaa agttttactg cttcagcttc tgtaaaatcc atatacaatg tttcaatatt
     1321 ttctttcact gcaccttcct gaagcatttt tacaaaaatt tcagcagact ttaccaaatc
     1381 tttatcatct ttatctgtac ctataataat tcgactcgga tataggttat catataacgc
     1441 ttttgactcc cgtaaaaact caggactaaa cataatattg tctgtaagaa atttcctccg
     1501 tactgacttt gtatatccaa caggaacagt acttttaatt attataattg catcaggatt
     1561 atatttcata actgcttcaa tgacagcctc gactgaagat gtatcgaaaa aatttttttt
     1621 cggatcataa ttagttggag ccgcaataac tataaaatcc gcattctcat atgcttcctc
     1681 tgcattcaat gtagctacga gattcaattc tttttccgcg agatattttt ctatataatc
     1741 gtcttggata ggggacttct ttttattgat taactcaact ttctcaggaa tgatatctac
     1801 ggcaacaact tgatgatgtt gagccagtaa gattgcaata gataatccaa catatcctgt
     1861 acctgctact gctattttca taattttttc ctttctcaaa acactctcgt tatacattat
     1921 agtagtcttt ataccactct gcaaactttc tcaaaccttc tcgtaaagaa gtttttggtt
     1981 taaaaccaaa atctctctct aaatcacttg tatctgcata agtaacagga acatcgcctg
     2041 gttgcatagg aactaatttt ttatgactat caaaatcata attttccggc aaaactcctg
     2101 ccttgattag ctcctgttga agaacatcca caaaatctaa caaattttcg ggtttactat
     2161 tcccaatatt gtacacttta tatggggcta acggtaaatt atcagatccc actagtttct
     2221 tcggagctga ttgcatgacc cttactatcc cctcaacgat atcatctata taagtaaaat
     2281 ctcgtttaca attaccatga ttataaatct caatatcgga accacttcgc aatttatctg
     2341 taaatccaaa ataggccata tctgggcgcc cagcaggacc ataaactgta aaaaagcgta
     2401 agcctgtcga aggaatattg taaatctttg aataagcata cgcttgaagt tcatttgttt
     2461 ttttagtcgc agcatatagt gaaataggat tgtctacctt atcttctgtt gcaaacggga
     2521 tttttttatt tccaccatat accgatgagg acgatgcata aactaagtgt tccacaccac
     2581 catcatatat ctcatacgaa tgacgacaag cttccaacac attatgaaag ccaacgatat
     2641 tactttctat atatacatct ggatttgtaa tggagtagcg tactcctgcc tgggcagcta
     2701 gattaacaac aatctgagga tgataactct taaaaatctt attaataagc attttatctg
     2761 caatatcacc cttgataaat atccagtttt tttctggata atcctgaatt agcttattaa
     2821 gttgaagcaa tcgataatct ttaagtgcaa tatcatagta atcattcaaa ttgtccaaac
     2881 caatgattgt catcgttcct tctaattcca ataatttcct aaccagattt gatccaataa
     2941 aaccagctac tcctgtaatt agaattgttt ttcctctcat atcataatac ctcttgttat
     3001 ttttatattt tcgtccacta atacacattc tttaacaact attataatca atttctaaga
     3061 taatctataa taaaaaacaa accacataca gtataagaga ttgctgatgc tatcgcagct
     3121 cctgtaattt ggtagttagg aattaagtac cagtttaaac ttatatttat caaagccgac
     3181 acagataaaa tttttatagc tacactctgt ttaccctgag atatataaat tggttgtaaa
     3241 attttaaaga aaatcatcga taagcttcct aaaaaaatga ctaatgttat aggaaatgcc
     3301 tttatataac tggaaccata aaggagcgta atcgcaaatt ctccaaaaga aagaaatcca
     3361 ataattaata aaatcataaa tagaaatgat acttttattg catttattgc tttatttttc
     3421 tcaaaagttc ttttagccga atcaccaaat aaaatctctt taaatgagtc tggaaccatc
     3481 aaaaacatat tagaaaacgt aactgcgaca ctgtatatac ctacattata actactagac
     3541 atttgcttca acattatgat atcaatacta taattaaatg taattaatag agtaagaatc
     3601 atagaaagaa atccaaactt atatatagtg aaaaattctt taatactaat atgttcaaaa
     3661 actgggaaca tattgtaata gtataaaaat aaaatgatac gtaaaaattc attaatagca
     3721 tataccaaaa ataccatctc tatagaaact aaatgaaata gtaacaacaa tactagcata
     3781 acggaatata ctacgcttac tcctaataca atcttattcc tctttatact attttctatt
     3841 actgcaatca tagtaagatt tgttctcata atagatagac tggacaaaac tattattacc
     3901 ccaccataat ctatcggaac atcagctaaa actattatta aacctattat tccaaacaat
     3961 aatccttgaa taaaagttaa aaaaacaaat ttattctgat actcattaga atgattcctt
     4021 ttaaaggtcg agtaagcctg acctaaacca actccaccta aaatataaaa aatttctact
     4081 aagttcatta cataggcata atctcctttt ttttcaacac ctaatcccct attaattata
     4141 attgaggata aaagtgttaa taagattaac aaaaatttac tcgttaaagc aaaccaatat
     4201 ttcttagata gtctcactta ttttctccgt atgttaaatt ttataacagt aaatttaaaa
     4261 ttatagtata ttgaaataga atatgaacaa aacgattagg gaatttaaag taatttataa
     4321 aaattcttta gaagctgcat tgtactactc caacttcaaa ccactatagt atcaaaaagt
     4381 ataccccacc tttttaaata aactactcac cacccatttc atccaatggt gatatcaaca
     4441 ccattctatt gataaaaaca ataaccaatg ttcccaatat agcatattta agtaatggag
     4501 ataatatgtt agcaaaatca gcacgagtag aataaataaa aataggtatc atatacagat
     4561 atatcagcat cccgtatata tttctttgag cgtttatata aagaaatctc atcaaaaagc
     4621 ctgttagcat aaaaatcatt acaattccaa agtatcctcc attcacatat ccctctccca
     4681 caagagtaaa accatatttg gttgaatgta cgtttggaaa aaaggtgtcc ataaaccaag
     4741 tttgattaga ataccccgta ttataaaaaa ttcttactat gtcattaaaa aaagaacgac
     4801 cttcgaaata attgcccata ttatgtaata tcagaatctg taaattacga ctagctgata
     4861 caaactcacc atctagcaaa tcaaaccaaa tattatttgt atttatagat gaaacttgcc
     4921 cacttaataa agtatattta aaaactgcag ataacggaat cagtagtgcc gcaagcggaa
     4981 ctaacattac aagataaaaa cgttttattt tgtgaaagta aaataatata atcacatcaa
     5041 ggatgaataa agtaaacata taatctcttt cccctgtaac gatagagaag agacagataa
     5101 ctatcgctgt gttagtatta caatttagta gtctccattt tttagtactc aactgtataa
     5161 atagctgata tgtatgaatg agaatcataa aataaaccaa agaaaatact aacattatca
     5221 caatattggc acctgaatat atttcccctt tatttctaaa accacttcct aatagatata
     5281 taattgctac tgttgtgata aacgtattca tattattgag aaataataaa tagttattgt
     5341 tatttaggtt tattttcctt tcgcttaaca caatggcttt agaaggtaga gttaacaaca
     5401 aagttgccaa agccagccat tgcatgaaaa tcaaatcttt tgtataacca tagtttatat
     5461 actccattct atagagtagc gggtaagaaa tagaataaat agtaaagaag ctactatacc
     5521 acacataggg atgaaaaata ttaaatttca caattatcat tgccataaaa cttaaaaaaa
     5581 tcattgaaaa taatggaatt gtaacatcat tattttcact gcataaaatt aaagtccctg
     5641 aaaaaactac acctactaga agtattattt tattttttat atcatttgat tgtagactaa
     5701 acttcattta cttttttcct ttaactaata atttaaacgt aatgcgtatt caaaaatttg
     5761 attggctatg tagtcagttt ttatagcata tttaatatat cgaaacggat tttccatcgc
     5821 attttaatct aaaccatcag aattatttaa ttctcttcta atttgagcaa aatagtcact
     5881 tctgcgttta tttaatacac tcgaaagaaa tttttttgag aattcaaact gttttatcac
     5941 catcttttcc ttatccatac taataaaatc tataatagat cccgcaatcg cttgtgaaga
     6001 ggatggagta aatagacaat gtggccccag caactctgga attcctgctt tattagaccc
     6061 tatacaaggg caagcacggc tcatagcttc aattaccgca cgaggtaacc cttcttgtaa
     6121 acttggttgt atatataaat caactttatc caaatactca taaatttgtg atgtagtcaa
     6181 ttcccctaaa aaatggattc tattcgctac tcccaattcc tctgctttct tttttaagaa
     6241 actttgatca ccatcacctg ctaaataata atccaacaaa ataccttgta tttctaatct
     6301 cttcattgct tccaatacaa atctatggcc cttggctcgt acattcacag atgctgttgt
     6361 cattattgaa attttttgag gattaaattt tttcaactta taaagacggc tcttcaaaga
     6421 tctattctct acagaggtaa tattaacatt tgaagcaacg atattagatt taatattagg
     6481 atatctatct tgtaaatatt tttctgtcac gtatatgcaa taattagcgt tttttacaat
     6541 tgatttagtt ttggcgtata tatatggagc taataatttt ccctttaaac tatgatacca
     6601 gtaagaatcc caagcatccc ccattatttc tgtcagatac ggcttaccta tcttcaatgc
     6661 gtagtctgca gtttttgatc caattataga cggaatacga acaactatta agtcgctatc
     6721 aatcattcct tcaataatct tatttttatt ttgaccaaat agaacctgat gtagaccttc
     6781 tatagggata aatttattaa taaagttcgc tttatgatat ccttcagggt atgattccac
     6841 cttctcataa cgactacata gtacaatctc tccaaatgct ttagcatagc gctcataaat
     6901 aaaataagct ttcggtttta aatatatttc atttttatag attgtacatc taaattcagt
     6961 cgctataaat actttcataa tttcatctcc cttgataaac gtttctgaat aaatatagta
     7021 tcagctggaa tatcctttgt tacaacactt cctgctgcaa ccacactatt ctcaccaata
     7081 ctcactccct tcaagacaat gctgttagct ccaatccaaa cattttctcc aatcataact
     7141 ggggccgtct taaatttttt tgctttcaat ccacctggaa cactaacatc atagtcatga
     7201 tcatatataa gtactccagg accacatttt actccagatt taatatctat gtatttacga
     7261 caagctatca tacaattatt actcaaaaat acatcatcag aaatcttgag ttttgctcct
     7321 tttctaactt tcaatactcc cccggcgttt actctaatct tttttcctaa gattaattta
     7381 gcattagatt cctgttcgac agttacattc tttccaaatt ttaaaaaata atgaaaagta
     7441 agatccttgt ttcgacacac ttttaataat atcaagcaaa tatttgcacg aagtaaacca
     7501 acaatatttt ttataatcct attcatctaa agctcctttt attttatcta aataagcatc
     7561 tataataatt tgacgagaaa aatattgctc tatatacatt ctcccttgaa gtcccatctc
     7621 ttttcgtttt tcattttcta gacctaaaaa aacttccaat atagagacta gttcctcaac
     7681 atttccccct tgaaatatat agcctgtctc tctatcttta acaatttctt tgcaacctgg
     7741 attatctgtc gtaattaaaa cacgtcctga actagcgttt tctagtaata cattgctcat
     7801 tccttcacca tacacactgg gatggataat tgcatgggaa cgggtaatat gaggtatcac
     7861 atctttttgt tgtcccaaat aataaacgat tccttttttt tctaagtcac aaattttaag
     7921 ttcataatta ctctctgtcg gttctataaa gccaataata ttaaactctg ttttgggata
     7981 tcgactctta ataatttggg cagcagccag ataagtatct ataccttttt cttttaatat
     8041 cctaccaata aaattaaaaa cgattgtttc tccttgaatt ccatttccac catttggata
     8101 ctgttgcaca ggatactttt caatgttcac tcctgaccca ggtatcagct tagagtcacc
     8161 caaaatcaag ccaagagttt ttaacatttt catattatat tcattttgaa aaaaaacaca
     8221 atttgatttc ctaaaagaaa ttctaaacag taataacacc aggcttctta cacaagaacc
     8281 cattgttaag acactaccta aacctgttac gttatttata tattttattc ctaaaattgt
     8341 tgcagctagg ctagcatata catttggttt cacggtatat gtcaagacta catccggtct
     8401 atattttctc aataatctta tataactaag aaatagacga atatctttta aagggtttat
     8461 tcctcttcta tcaatagtaa tatcttcata ctcaaattga atagacttca tcaactctat
     8521 cttatctcca taaggacatg acactagcac ttcataccca tactcaacta gtgactctat
     8581 aacttctttt cgaaaacaat agatatcatc gtcatgatta gttattaaca taattttttt
     8641 cttttgtaaa ttcatcaaaa taatcttcct aaaatataat taaccgctac tccattatcc
     8701 tttacaacta tcattttaaa aatagtaatt ttctataagt aattgtttcc aaattgtgac
     8761 actttaaaat tctatacaca tatagaactt acaaactatt tacgaccgta aactccatac
     8821 ttatcaaccg agatatccaa tttatttaaa acaactccca ggaacagttc ccctgtttgt
     8881 tctaattgtt gtttcgcttt ttggacatca cgtttattcg cctcacctgt tgctgttatc
     8941 aagatggacg catcacactt ttgagtgata attgccgcat caataacaat tccaataggt
     9001 ggtgtatcaa caatgatata atcaaaatat ttacgcaatg tttcaatcat atcattaaaa
     9061 tttttacttt gtaacaaggc tgtagggttt ggtgatacag atcccgattg aactacaaat
     9121 aaattttcaa tatttgtatc acataaaccg tgagataaat cagctgtccc agataaaaat
     9181 tctgttagcc ctgtaatttt ttcacgagat ttaaaaaatc ctgacataac tgaatttcga
     9241 atatcgccat cgaacaaaag agttttatag cctgcacgcg caaatgacat tgctatattt
     9301 acggaagtag ttgtttttcc ttcccccgga ttaacagaag taacggaaat tacttttagt
     9361 ttatctccgc tcaactgtat atttgtacac aaggtattgt aatattcttc tgccttctta
     9421 atgaactcca gttttttttg tgctatttct aatgtcggca tctctctctc ctatttcaac
     9481 ttacccaagt ttggtacaac tcccaaaagt gtcatctgca gtgcattttc gatatcttcc
     9541 ggacgtttca cacgagtgtc caaaagttca agaagaagaa ctataacact agttccaatc
     9601 acccctgcca aaaaaccaat tagtgtattg cgtttaatat ttggcgaaga cggtgatatc
     9661 gccggtcttg cctcctccag tgttgtcaca tcagaaacac gagtaatact gataattttt
     9721 tgagcagcta cttctcttaa agagttagcg atacggcttg cctcttcagg aactcgatca
     9781 ctaactgaaa cagagacaat acgggtatca actggtactg ttactttaat tttattagcc
     9841 aaaccttttg gcatcaaatc tagtttcaaa tcagaaataa cttcctccaa aacatcctgc
     9901 gataggacaa tttcacgata gtctttaacc aagtatgctc ctgcctgcaa gtcttgattg
     9961 gttaaaccag gcttctctcc ctgattacgg ttaactagat aaatccgagt cgtactagta
    10021 aactcaggtt tgataacaaa agtactgtag gcaaaagcaa ctgaagaagt tataattgcc
    10081 actaataaaa tgaccaactt tcttttccat aaagctctga atagttgcaa tacatcgatt
    10141 tccaaagtgt tttgttcctt catattttct cctaaattaa ttgatccatt ataatttttc
    10201 tgggattatc tacaaaaagt tctttcgctt ttttcgctcc atatttctta gcaatgatat
    10261 catatgcctg ttgcatatat ggaggtctac tgtctaaatt gtgcatgtca cttgcaacta
    10321 catgaactaa atcacgttcc aaaaaatacc gagctctctt tttcatgaat ttatattttt
    10381 caccaaagaa cttaggtttt gaaacatgat aactatttat ctgagtatag caccccatat
    10441 caatcagttc acgaacacgt ttttcgttat tctctaaagc atcataacgt tcaatatgag
    10501 caattactgg cgtgattccc aacatcaaaa tattgctcaa tcccgtatga atctgacgat
    10561 aggaagtatg catgctaaac tcaatcaagg cataacgact atcattaagg gtaggaattt
    10621 cttttttttc tagcttttct agagcatcca gagtatagta tatctctgcg ccataagcaa
    10681 tgactaaatc atctgctact tcttttgcaa tttcccgaac ctgaagaaag ttttctgcta
    10741 tcttctcttc cggagtttca aacatgccct tgcgacggtg agaggtagag acaatggttc
    10801 tcaccccctg cctgtaggat tctgccaaga gagccttgct ttcctctctt gacttgggac
    10861 catcatctac atcaaagacg atgtgcgaat ggatgtctat cattttatct accctccatc
    10921 acatcctgta tagctgcttt aactacagtt aaactactat catctatttc catcacatag
    10981 aggttactgt ctggcattgc ataagaagga agatccatcc gacctgtccc ttttaaatct
    11041 tgagaattta ctttataatt ccctccactt tccaactgag tattcactaa atctatcata
    11101 gtctcaatcg gtatatttgt ttgaagagaa tcctgcaatc cttgaagaat actactatag
    11161 tttttcaaaa cctctgtaga agttaacttc tgaataattg ctacaatgac cttttgttgg
    11221 ttgcgaccac ggtcacggtc tccatcggtt agtgagtagc gttcacgtac aaaacctaaa
    11281 gcttgctcag agtctagatg gacattccct actgggaaat ggaacttccc atgtagagct
    11341 gaaaactctt gatcattatg aacatctacc cctcccaata agtcaatcat tttcaagaaa
    11401 gaagtgaagt tcaatcgcac atagtaattg atatccactc catagagatt ttctaaggtg
    11461 tgaatggacg aatcaactcc ataaatgccc gcatgggtta atttatcttt ttgattattt
    11521 ccactatctg cgattggtac ataggcatca cgtggcgttg tggtcaagag gattttcttg
    11581 gtatctcgat tgacagtcat taggatattg acatctgatc gcgacaccga actaatagga
    11641 ccataggtgt caattccact aacatagata ttgaaagact gattcttaga cgtcttagga
    11701 gcctctactt ttttagtgaa tcccttggta taaatctttt ttatcttcga tgcgtagtct
    11761 ggatactctg actcgatgat attttcaaag acactattta ggacaatggc cttagtctcc
    11821 cctgcaatca aactcttgta agctgccaag taagacgaac tctggttgac cgtcaaatcg
    11881 gtattctgac ttgacttgat atcagctagt agtttttgaa tattttcatt atcagtccca
    11941 gtcggtgctg tcacactcgt cagttgcgta acattttcga tcttactatc tgctaaaaca
    12001 gcgacactga ttgaatattc tgagtaatta gaagtcgcat ttaaacgatt ggtcagtcca
    12061 acaaactgct gtactgcaaa gagcgacact gagctgacaa ggatagagaa cagcaacaga
    12121 aaaatagtaa acttttcagc ttttttatag ataatcaaga gtagccctac caaggcaact
    12181 agtaggacta acgcagttac cactaggtta agatatctaa aagcaaggat attgtactta
    12241 aagattaaga acaataaaaa acaaattaac aataaataaa tagtcagcaa aacgatatta
    12301 acacttcgct tcactttctg tgaacgtgat tttttaaaac gtctactcat gattaacacc
    12361 tataccttga acatcataca attatatcac ttttttacgg taatgtctac acctttattt
    12421 tcactatctg catctttaag tatcttagta gacttcccgc gaaccaaaaa tatagtaaaa
    12481 tgaaataaga ataggacaaa tcaatcagga cagtcaaatc gatttctaac aatgttttag
    12541 aatcagatgt gtactattct agtttcaata taa
//
LOCUS       DAVBBS010000055        12554 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVBBS010000055 DAVBBS010000000
VERSION     DAVBBS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12554
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.055"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            2..1242
                     /locus_tag="VQU32_001682"
     CDS             join(2..341,341..1242)
                     /locus_tag="VQU32_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2473104.1"
                     /translation="MVKLIDRYGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSIS
                     LDYALPNQGMLSFWPAQYKKNGYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENE
                     RLRTEVAYLKKFKRVRGKGRSPRARKAETVREMVSGGFRLDFLLETARLARSTYYYQL
                     KQLDGVDKDKEIKTEIQGIDNEHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLT
                     ARIRRKRKYSSYQGEIGKKAENLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPV
                     LDGFNSEIIAYHLSTSPNLEQVKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLE
                     SKGIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNN
                     KRIKVKLKGLSSVQYRTKSFG"
     gene            complement(1477..2085)
                     /locus_tag="VQU32_001683"
     CDS             complement(1477..2085)
                     /locus_tag="VQU32_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HET2473105.1"
                     /translation="MQIKFIDKVSNLIMLNLLYVASVVTVIAIGSGESALIATLIKIV
                     RHEESYPYRDFANSFFKDYWKNLGAALISNLPILILLFSLFFLPYIPLPIYIISILRH
                     IGVIYIILHLIATTFLIPLIGRYNNTLKNSLHNSIMLAYKHFFIAVLIRIIEIIPVLL
                     FFILQNQLLVWITLMIFILPSITKYANAFLYNFIFSKYEKLN"
     gene            2251..2421
                     /locus_tag="VQU32_001684"
                     /pseudo
     CDS             2251..2421
                     /locus_tag="VQU32_001684"
                     /inference="COORDINATES: protein motif:HMM:NF013577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            2410..3021
                     /locus_tag="VQU32_001685"
     CDS             2410..3021
                     /locus_tag="VQU32_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2473106.1"
                     /translation="MTKDILELESQKMSSDTFIDEIKNNYLSIVESTRKLIDGRQIEL
                     AIKLIREANQILMIGVGSSGNAAREFESSLLRIGIISKTVIDTHFQLMHTALLKDNDL
                     IIAFSLSGSTKEVEETLLNAKRKNVKIISITNYSSRNIAKLSDCVLLTSKKESYLEGG
                     SLMAKASQLFIIDVICTRLSLINYEDTICKKEEIASLLSNKVE"
     gene            3738..4424
                     /locus_tag="VQU32_001686"
     CDS             3738..4424
                     /locus_tag="VQU32_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2473107.1"
                     /translation="METKKIKNLKGQIIVSCQALEGEPLYTPNGGVMPLLAKAAFQAG
                     AKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILAL
                     DCTNRSRPGYDNITDFIHDIKVKYPNQLLMAAISTFEEGKVAAESGVDFVGTTLSGYT
                     PYSPKKDNPDFELVERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPL
                     EIAKKFIEVV"
     gene            4425..5357
                     /locus_tag="VQU32_001687"
     CDS             4425..5357
                     /locus_tag="VQU32_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2473108.1"
                     /translation="MNQEILKKSKIQLTVEHIQKYWILYLMMIPATVLLILFTYGPMY
                     GIIMAFQDFTVFKGYTGSPFVGLKHFQRLFSDPLFYRLFKNTFMVGVLDFLFFFPAPL
                     IFALILNEVRKVRFKSVVQSISYLPHFIPLVVMVGIIFELFGSYGIINSLLSSLGMEP
                     ISFFTKSEWFLPLYIGSGVWKTIGWGSIIYMGALTNIDSTLYEAADMDGANRWHKMWH
                     VTLPSLRPTVVTLFILNAGGIMQVGFEKVFLMSSPATYEVSDVLSTYVYRQGILNSDF
                     SYSAAVGLFNNIVALLFVLLANKIAKKLGEEGIV"
     gene            5368..6297
                     /locus_tag="VQU32_001688"
     CDS             5368..6297
                     /locus_tag="VQU32_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2473109.1"
                     /translation="MIFSKNTKKSNRDKETFVDYLFIFILIIITLLMLYPLWWVVMTS
                     LSEPVWLSKNVVKLFPLKIDITAYKFIFGDSKLWQAYGNSVLYAALSTIVSVAVCSMY
                     AYPLTIPEIRGKKFWNTLILIPMFFSGGMIPLYIQVANLGLLNTAWALVLPGAVSTYY
                     IILFRTQFKSIPMELRESARIDGASEFRIFWNLVLPLSKVIFMIIALYCIVGSWNSFT
                     PPLLYLNDDTKMPLSIYLRNLVVTNTTDTAELSLSAGNTYYQQIAAQGGGIGMRIAIK
                     MGTIVVSVIPIMMIYPFIQKHFVKGILLGSVKG"
     gene            6311..7750
                     /locus_tag="VQU32_001689"
     CDS             6311..7750
                     /locus_tag="VQU32_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473110.1"
                     /translation="MQNLKKYLLLDSGLVVGYTLENAQLKLNKLKKDTENNPLFGEEY
                     FAENPKLWEVRFDNSYPNVIYDKELKLYRCWYQTFVSDEASEETPLAERGEKEYIVKS
                     SRMTALCYAESKDGVKWEKPNLNLVKFKGSKDNNIVLMHAHGAGIMLDEQELDPKKRY
                     KLVSKLEFSPNLHYMVVVFSEDGIHFDEPIPWKKWNPAADSHNFPFRDPKTGKYAVIT
                     RTWANNVRLSAITFSDDFINWSEPKEILRGDGFEDQIYSMPVISYADIYLGFASMYHE
                     GDTLNENYDKVDLELKFSANLEQWDSVCKGDYLIERGGGVYPQNADFDASCIFTAAPI
                     IEDDKIWFYYMGGNGNHTGFRESSLARGYIKKDQFAYYTNKREGIPAKISTVSFQTYD
                     DEIYILADIPENGYIKVAVGTKYGNAYSDFGQEDISLVQVSDGKYKLEFNKKSILDLG
                     NSPVAFKFEIENAKLYGIEGSIQQHYLKY"
     gene            7819..9396
                     /locus_tag="VQU32_001690"
     CDS             7819..9396
                     /locus_tag="VQU32_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2473111.1"
                     /translation="MVKLNKVLKGSVAFLSASLLLAACGNNANSKPAEKTADGKTKIS
                     FLSTWQINDVRPPKDETLVGQWLEEQLGIDIDLVTIPGEGVSSKVNSLITSNQLPTVL
                     LTTGDKSDIAGINKLGKQGAFLDLSQHMDKLPNYKKYLEKNNALAQIEDDEKHIYAFT
                     KFFTDENIMYTTPILRKDLLVGSEFEDLSKIKTVDDYTKVLKYLTEKQGSPAFIQRNG
                     YEGFMKRVTPLWNLSHRTYYDYESDSYKHPVEQPQLKQFVEWLKELRKDNVLHPDWAV
                     MKDETWEGLLASNKGSFTVDRMNIIGDNNFDKSFDWQPLVYPEINGKRYLQPKTPYIS
                     DSGWVINANAPKEEIDKALEFFNFLYADENQEKLAIGFEGKTFTRENPNTQAGIKWLI
                     QIYGENAGNDKAELLFKHGNQAFTRVQTKLDIEAQPGTFPKVMYDQVDKIKKDLGGFR
                     PDTPVLSFTTEELDVLTKSEAGLNTFFDENIIKFIEGQRDMSEWDSFINEAKSKDLDK
                     VTKVYNDAYKRVKDRMK"
     gene            9456..10916
                     /locus_tag="VQU32_001691"
     CDS             9456..10916
                     /locus_tag="VQU32_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET2473112.1"
                     /translation="MYDVVIIGAGSSGALAAIASGRLGLKTCLIEKGSRIGGVPINTL
                     MGSFANLYFDDSGRVNASKIVGELIERIIKKGGTVFNSMEELMNVNDFYQITIPYQPE
                     VYESVLTDMLIESKVDILLNVEVNNIIYDSKNIKSLEILSNQEVKLIDSKVFIDATGN
                     ATIASASGADVYEKNSSYGCLMRVGGVDISKTIEHIEKTKQWNYIEGYNQWLEEVLAN
                     RKYRATGAVLKDPVSYDHAPMLSKDDHFMNDEKWNYIKERWEKFRFIYTLEVSLFRNE
                     LKKAVESGDFNFEIYHDDKSGVTMNGDGISYGSWGQNVALINVAKAFGFNPKYMEDES
                     HANILSRKYNLTFLSFLKKYVPGFENSFILDQGTRATNRSCRHIDNSSMMDEILDFPM
                     YTFKTMYSYQTPKEITYKSIVGGKLNNLFVIGKGAYQSESFRSQISCMLMGISSAAAA
                     KTIIEDKSNTLNINRDLFKSNLDILFTWEFSNWITD"
     gene            10938..11393
                     /locus_tag="VQU32_001692"
     CDS             10938..11393
                     /locus_tag="VQU32_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET2473113.1"
                     /translation="MNDTNKNQYFVYEFDTSFVEMSYSQFFEGYCILFSKEPYPSLSD
                     MPMEFRKEFLMEMSILGDAMLEVLNAYRINYDILGNSTPVLHAHLFPRYMWEEEERRK
                     TVVHRYDSSVFSNPEFEFTIEKHGPIIKKLRSAIDRHYREYQQLLDDSN"
     gene            11480..11885
                     /gene="cdd"
                     /locus_tag="VQU32_001693"
                     /pseudo
     CDS             11480..11885
                     /gene="cdd"
                     /locus_tag="VQU32_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221146.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
     gene            11898..12347
                     /locus_tag="VQU32_001694"
     CDS             11898..12347
                     /locus_tag="VQU32_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2473114.1"
                     /translation="MELVEFKNGHVNTVHEHIAITKVKEFLNNHDILKDKGGIHKIST
                     DFFYNIIDMETTTEENRPWESHKEYYDVHYLLNGEETILYNFLSQMELSEYKVDDDWQ
                     QMNGTALFSIKLKKDMLLLLEPNDAHRTGLLVEEPLNIKKVVFKVKI"
BASE COUNT         4477 a         1627 c         2312 g         4138 t
ORIGIN      
        1 catggtgaaa ttaattgacc gttatggcat agaaatcgtt aaaaaaggga agaatcgtca
       61 ctattcttct aaattgaaac aagaaatgat ggataaagcc ttactggaag gttgttcaca
      121 aagaagtatt agtcttgatt atgccctccc aaaccaagga atgctttcat tttggccggc
      181 acaatacaag aaaaacgggt atactattgt tgagaaaaca agagggagac cagctaaaat
      241 gggacgtaaa cggaagaaga cttgggaaga aatgacagaa ctagaacgac tccaggagga
      301 gaatgaacgc ttacggactg aggtggccta cctaaaaaag ttaaaagagt tagaggaaag
      361 ggacgaagcc ctagagcgag aaaggcagag acagttagag aaatggtttc aggaggattt
      421 cgactagatt ttcttcttga aacagcacgt ttagctcgct cgacttacta ttatcagttg
      481 aaacaactgg atggggttga taaagataaa gagattaaaa ccgaaattca gggcattgat
      541 aatgaacata aaggcaacta tggctatcgt cgaatccatt tagaactaag gaatcgtggt
      601 tttgtggtca atcataagaa agttcaacgg ttgatgagaa ttcttggttt aacggctcga
      661 attcgccgta aacggaagta ttcttcctac caaggagaga ttggcaagaa agcagagaat
      721 ctcattcaac gccagttcga agcatcgagg ccaatggaaa agtgctatac agatgtgaca
      781 gagtttgcca ttccaaatag cacacagaaa ctctatttat caccagtttt agatggtttt
      841 aacagtgaaa tcattgctta tcatctttct acttcgccaa acttagaaca agtgaagagt
      901 atgttagaac aggcctttac agagaaatac tatgagaata cgattctcca tagtgaccaa
      961 ggctggcaat accaacacga ttcttatcat cggttcctag agagtaaggg aattcaagca
     1021 tccatgtcac gcaagggcaa cagcccagac aacggcatga tggaatcttt ctttggcatt
     1081 ttaaaatccg aaatgtttta tggctatgag aagaacttta gatctttaga aaaccttgaa
     1141 caagctattg tggactacat tgattactac aacaacaaac gaattaaggt aaaactaaaa
     1201 ggacttagtt ctgtgcagta cagaactaaa tcctttggat aaattatttg tctaattttt
     1261 tggggtcagt acagtattaa ataccatgat cttatgcgtt ttataataaa aaatctgttc
     1321 ttgttttatt tttagaaaat ttactaaaga ctaaaaatat tatgaataat ttaaaaaata
     1381 gtcaggttat atagtttttt gaaattaaaa ttattttatc gttgaaatta gttaaaaatt
     1441 tttataaaat caagatgcaa ggctatgaga tgttctctag tttaattttt catacttcga
     1501 gaatataaag ttatataaaa atgcattagc atattttgtt attgagggaa gtataaaaat
     1561 cattaaagtt atccaaacta gtaattgatt ctgtaatata aagaagagta gaactgggat
     1621 aatttcaata attctaataa gaacggcaat aaaaaaatgt ttataggcta acatgattga
     1681 attgtgtaga gaatttttta aagtgttatt atatctaccg attaaaggaa ttagaaatgt
     1741 tgttgctatt aaatgaagta tgatatatat tactccaata tgtcgtaaga tagaaatgat
     1801 atatataggt aatggtatgt agggtaggaa gaacaaactg aataataaga ttaaaattgg
     1861 taaattagat attaatgctg ctcctaaatt tttccaataa tctttaaaaa aactgtttgc
     1921 gaagtctcta tagggatagg attcctcgtg tcgtacaatt tttattagag ttgctattag
     1981 ggcactttca cctgatccga tagcaataac tgttactact gacgctacat agagtagatt
     2041 taacattata agatttgaaa ctttatcaat aaattttatc tgcatcttta actcctgctg
     2101 tttttatact tataatatca catcatttga gtttcatcaa ctttgaatac attaagaaag
     2161 aatataacta tgaggaaagg aaaaaattat tatgaataaa tccaaaaaat ataaagaaag
     2221 aattcattat gaatacgata atcttactaa atcagagaaa aaattgctga ctatatttta
     2281 gttagagata gtgaaatagt ttatgaaacg tcttcagaaa ttgttagtaa agtaaaagtt
     2341 ggagagggaa ctcttaatcg attttgcaaa aagattggat actctggttt tcaggaacta
     2401 aaattaaaaa tgacgaaaga tatattagag ttagaatctc aaaaaatgag tagtgacaca
     2461 tttattgatg aaataaaaaa taattattta tctattgtgg aaagtacaag aaaattaatt
     2521 gatggtagac aaatagaatt agctataaaa ttaataagag aagcaaacca gattttaatg
     2581 attggtgtgg gttctagtgg aaatgcggct agagaatttg agagtagttt gttaagaatt
     2641 ggaataataa gtaagacggt aattgatacc cattttcaac tcatgcatac tgcgttattg
     2701 aaagataatg atttgataat agcgtttagt ttatctggtt ccacaaaaga agttgaggaa
     2761 actttattaa acgcaaaaag aaaaaatgtt aaaattattt caataacaaa ttattcgtcc
     2821 agaaatatag caaaattatc tgactgcgtt cttttgacat caaaaaaaga atcttattta
     2881 gagggtggta gtttaatggc taaggcttct caactattca taattgatgt aatatgcact
     2941 agactctctt taataaatta tgaagatact atatgtaaaa aagaagagat tgcttctttg
     3001 ctgtcaaata aagtggaata aatcaataat ttttaaattt taataaaaat tttttaaaat
     3061 aaattaaaaa acgatcctaa aagggtcgtt tttttgctaa agttttaaaa ataaacaata
     3121 attaagatta tagtgcgttg aatctatatt aggacataac aattcttaaa tcattgttag
     3181 aaattagttt gaattttcca atcaatttgt tcgattttta catcattgca ttatatcacg
     3241 tgactattaa ccttgttttc tcctaaaatt gattttcttc ttttcttatc ttgtccactc
     3301 gaaacaagta ttgtaagaat ttgattattt ttgaaagtac ttttaatata tttgagatag
     3361 ttaaaaaaga tttgaaacta aattccaaat tagaaaaaga cttgaaatgc taaaaaaaaa
     3421 aagtatactc taattgaaaa cggtaacaaa actaatttag agaatgaaat atagagtatt
     3481 tctctcttaa aagtttttgg tgaaacgaga tgtagaaagg agatttagcc aaagagtcta
     3541 ttagtgctag aataatagat tagaattata gtgtattgaa tctataacag tacgccttaa
     3601 atgctaaaat atttctataa attaatttaa ctttcctgat agggctgttc atatcttatt
     3661 tcaattcact atataaataa aattgtagta gatgttattg aattagtaaa aaaaataaaa
     3721 aaacggaggt tttctgaatg gaaactaaaa aaattaaaaa tttaaaaggt caaataattg
     3781 tttcctgcca agcgttagaa ggagagccac tttatactcc aaatggagga gtgatgccgt
     3841 tattagcaaa agccgccttt caagcaggcg ctaaaggtat tcgtgctaat tctgtccgcg
     3901 atatttcaga aataaaagaa gaagttgact taccaattat aggtatcata aaaagagatt
     3961 acgatggctt tgaacctttt atttcagcaa ctatgaaaga gattgatgaa ttagttagtg
     4021 aaggagtaga cattttagct ttggattgta ctaatcgttc aagaccaggt tatgataata
     4081 ttactgactt tattcatgat attaaagtca agtatccaaa ccaattatta atggctgcta
     4141 tttctacttt tgaagaaggg aaagtagctg ctgaatcggg agtagatttt gtgggtacga
     4201 cattatctgg ttatacacca tatagcccta aaaaagataa tcctgatttt gagttagttg
     4261 aaagattggt aaaagagctt gacgtaccag ttattgctga aggaagaata agtactccgg
     4321 aacaagctag aaaaatgtta gatttaggtg cttacgcagt ggttgttgga ggagctatca
     4381 ctcgtccttt ggaaatagcg aagaagttta ttgaggtagt ttagatgaat caagaaatat
     4441 taaaaaagag taagattcaa ttgacagttg aacatattca aaaatattgg attttatatt
     4501 taatgatgat tccagcaact gttctactaa ttttatttac gtatggacca atgtacggca
     4561 ttattatggc ctttcaagac tttactgtct tcaagggata tactggaagt ccatttgtcg
     4621 gtttaaaaca ttttcaaaga ttgttctcag atcctttgtt ttatagattg ttcaagaata
     4681 cgttcatggt aggggtttta gattttttat tctttttccc agctccgctt atttttgcac
     4741 tcatattaaa cgaagtcaga aaagtaagat ttaagagtgt agttcaatct attagttatc
     4801 ttcctcattt tattccccta gtggttatgg ttggtattat ttttgaacta tttggttcat
     4861 atggtattat taacagttta ctttcatcac ttggaatgga gccaataagt ttctttacaa
     4921 aatctgaatg gttcttgcct ctctacattg gttcaggagt atggaaaaca ataggttggg
     4981 gttctatcat ttatatggga gcattaacca atatagattc aactttatat gaagcagcag
     5041 atatggacgg ggctaataga tggcacaaaa tgtggcatgt aactctacca agtttaagac
     5101 ctacagtagt tactttgttt atactaaatg ctggtggtat catgcaagta ggatttgaaa
     5161 aggtattctt gatgtctagt cctgcaacat atgaagtatc tgatgtatta tctacttatg
     5221 tttatagaca aggtatttta aattctgatt tcagttactc agctgcagta ggattattta
     5281 ataacatagt agctttgtta tttgttttat tagctaataa gattgctaaa aaacttggcg
     5341 aagaaggaat tgtataaggg gggtaaaatg attttttcaa aaaatacaaa aaagagtaat
     5401 agagacaaag aaaccttcgt tgattatctc tttatattca ttttaataat aataacctta
     5461 cttatgttgt atccattatg gtgggtagtg atgacgtcac tttcagaacc agtttggcta
     5521 tctaaaaacg ttgttaaatt atttccctta aaaatagata ttactgcata taagtttatt
     5581 tttggggatt ctaaactatg gcaagcatat ggtaatagtg tattgtatgc tgcactatct
     5641 acaattgtta gtgttgcggt atgtagtatg tatgcctatc cacttacaat tcctgaaatt
     5701 aggggtaaaa aattctggaa tactcttatt ttgattccaa tgttctttag tggaggaatg
     5761 atcccgcttt atatacaagt tgcaaacctc ggtttattga atactgcttg ggctcttgtt
     5821 ttacctggtg ctgtttctac atattatata attttgttta gaacacagtt taaatcaatc
     5881 ccaatggagc ttcgtgagtc tgctagaatt gatggggcaa gtgaatttag aattttttgg
     5941 aatttagtat taccattatc aaaagttatt ttcatgatta tagcacttta ttgcatagtt
     6001 ggttcatgga atagttttac tcctccatta ctttatttga atgacgacac taagatgcct
     6061 ttatctattt atttgagaaa tttagtcgtt acaaatacaa ctgatactgc tgaattatca
     6121 ctttctgcag gtaatacata ttaccaacaa attgctgctc aaggtggagg tattggtatg
     6181 cgtattgcta ttaagatggg tactattgtt gttagtgtta ttccaattat gatgatttat
     6241 ccatttattc agaaacattt tgttaaggga atcctgctag gttctgtgaa aggttaataa
     6301 ggtaattatt ttgcaaaatt taaaaaaata tcttttatta gatagcggtt tagtagttgg
     6361 gtatacatta gaaaatgctc aattaaaatt aaataagcta aaaaaagata ctgaaaacaa
     6421 tccactattt ggtgaagagt attttgccga aaatcctaaa ttatgggaag ttcgttttga
     6481 taattcatat ccaaacgtta tctatgataa agaattgaaa ttatatagat gttggtatca
     6541 aacatttgtt agtgatgaag caagcgaaga gactccttta gcagaacgtg gagagaagga
     6601 atacattgtc aagtcatcta gaatgacagc actttgctat gctgaaagta aagatggagt
     6661 aaaatgggaa aaaccaaatc tcaatctagt aaaatttaaa ggttcaaaag ataataatat
     6721 tgttctgatg catgcacacg gtgcaggtat catgctagat gaacaggagt tggatcctaa
     6781 gaaacgatat aaattagttt caaaattaga attttctccg aatcttcatt acatggttgt
     6841 tgttttttca gaagacggta ttcattttga cgaacctatt ccatggaaaa aatggaatcc
     6901 tgcagcagat agtcataatt ttccatttag agatccaaaa actggtaaat acgctgttat
     6961 tacaagaacc tgggcaaata acgtacgtct aagtgcaatt actttcagtg atgattttat
     7021 taattggtca gaacctaaag aaatccttag gggagatgga tttgaagatc aaatttattc
     7081 tatgccagta atttcatacg cggatatcta tttaggtttt gcttcaatgt accatgaagg
     7141 cgacacttta aatgaaaact atgataaagt agacttagaa ttgaaattct ctgctaattt
     7201 ggaacaatgg gatagtgtat gtaagggcga ctatctcatt gaaaggggag gtggagtata
     7261 cccgcagaat gctgattttg atgctagttg tattttcacg gctgctccaa taattgaaga
     7321 tgataagatt tggttctact atatgggtgg aaatggtaat cacactggct tccgagaaag
     7381 ttcattagct agaggatata ttaagaaaga ccaatttgct tattatacta ataagagaga
     7441 aggaattcca gctaaaatat ctacagttag cttccaaact tacgatgatg aaatttatat
     7501 cttagctgat attcctgaaa atggttatat taaagttgcg gtaggtacta aatacggtaa
     7561 tgcttattca gatttcggtc aggaagatat ctctcttgtt caagtttccg atggtaagta
     7621 taagttggaa tttaataaaa aatctatctt agatttaggt aattctccag ttgcgttcaa
     7681 atttgaaatc gagaatgcaa aattgtatgg tattgaaggt agtattcagc aacactatct
     7741 caaatattaa ccagaataat tcgttaacaa ataatctcta tattagtgga gatagatata
     7801 taaaatggag gtcttattat ggtaaagctt aataaggtat taaaagggag tgttgcattc
     7861 ctgagtgcaa gtttattgct agcagcttgt ggcaataacg ctaattcaaa gcccgctgaa
     7921 aaaacagctg atggtaaaac gaaaatttcg tttctatcta catggcaaat taatgatgtt
     7981 cgtccgccta aagatgaaac acttgttggt caatggttag aagaacagct aggtattgat
     8041 attgatcttg taactattcc tggcgaggga gtaagttcta aagtaaattc tcttattact
     8101 agtaaccagt taccgactgt tttgttgaca acaggagata agagtgatat cgctggtatc
     8161 aacaaacttg gtaaacaggg tgcattctta gatttgtcac aacacatgga caaactacca
     8221 aactataaga agtaccttga aaaaaataat gcattagctc aaatagaaga tgatgaaaaa
     8281 catatttatg ctttcacaaa attctttact gatgagaata tcatgtatac tacaccaatt
     8341 ctgagaaaag acttacttgt tggttcagaa tttgaagatc tttcaaaaat taaaactgtt
     8401 gatgactata caaaagtttt gaaatatttg actgaaaaac aaggttctcc agcatttatt
     8461 caacgtaatg gctatgaagg cttcatgaag cgagttacac cattgtggaa tttaagccat
     8521 cgtacatact atgactatga atcagattca tataaacatc cagttgaaca acctcaattg
     8581 aaacaattcg ttgaatggtt gaaagaatta cgtaaagata acgtacttca ccctgactgg
     8641 gcagttatga aagatgaaac ttgggaaggt ctattggctt ctaacaaggg ttcattcact
     8701 gttgaccgta tgaatatcat tggagataat aactttgata aatcatttga ttggcagcca
     8761 cttgtttatc cagaaattaa tggaaaacgt tatttgcaac cgaaaacacc gtacatttct
     8821 gattcaggtt gggtaatcaa tgccaatgca ccaaaagaag aaattgataa agcgttagaa
     8881 ttctttaatt tcttatacgc tgatgaaaat caagaaaaac ttgctattgg atttgaaggt
     8941 aaaacattta cacgtgaaaa ccctaatact caagcaggta ttaaatggtt gattcaaatt
     9001 tacggtgaaa atgctggaaa tgataaagca gaactgctgt ttaaacatgg taaccaagca
     9061 tttactcgcg ttcaaactaa attagatatt gaggcacagc caggaacatt ccctaaagtt
     9121 atgtatgatc aagtagataa aattaagaaa gatttaggtg gttttagacc agatactccg
     9181 gttctttcct ttacaactga agaattagat gttttaacta aatctgaagc aggattaaat
     9241 actttctttg atgaaaatat tattaaattc attgaaggac aaagagatat gagtgagtgg
     9301 gattcattta tcaatgaagc taaatctaaa gacttggata aagttacaaa agtatataat
     9361 gatgcctaca aacgtgtcaa agatagaatg aaatagacta aatggtagct ctctggtttt
     9421 tcagagagct actactgcct gagggggagt aagctttgta tgatgtagta attataggtg
     9481 caggaagttc gggcgcattg gctgcaattg cttcggggcg tctagggtta aaaacttgtc
     9541 taatagagaa gggaagtaga attggtggag ttcctattaa tacactaatg gggtcatttg
     9601 ctaatctata ttttgatgat tctggtagag taaatgcaag taaaattgtt ggggaattaa
     9661 tagaacgtat tatcaaaaaa ggtggtactg tctttaactc tatggaagaa ttgatgaatg
     9721 taaatgattt ttatcagatt acaattccct atcaaccaga agtttatgaa tcggttttaa
     9781 ctgatatgtt gattgagtct aaagttgata ttttactaaa tgttgaagtt aataatatta
     9841 tttatgatag taaaaatatt aaaagtcttg aaattttaag taatcaggaa gttaaattga
     9901 ttgattctaa agtttttatt gatgctacag ggaatgcaac aattgcttct gctagtggag
     9961 ctgatgttta tgaaaaaaat tcttcttacg gttgtttaat gagagttgga ggagttgata
    10021 tctcaaagac tatagaacat attgaaaaaa caaagcagtg gaactatata gaaggatata
    10081 atcagtggtt agaagaagta ttagcaaatc gaaagtacag agccactgga gcagttttaa
    10141 aagatccagt ttcttatgat catgccccta tgctttcaaa agacgaccat tttatgaatg
    10201 atgaaaaatg gaattacatt aaagaaagat gggaaaaatt tagatttatt tacactttag
    10261 aagtatcact atttaggaat gaattaaaaa aagctgtaga atctggggat tttaattttg
    10321 aaatttatca tgatgataaa tctggagtca ctatgaatgg cgacggtata agctatggtt
    10381 catggggtca aaatgtagca ttaattaatg ttgcaaaagc atttggattc aatcccaaat
    10441 atatggaaga tgaatcacat gctaatattc tttctagaaa atataattta acatttttat
    10501 cttttcttaa aaaatatgta ccaggttttg aaaattcttt tatccttgat cagggaacta
    10561 gagctactaa cagatcatgt aggcacattg ataattcatc tatgatggac gaaattcttg
    10621 attttccaat gtatacattt aagactatgt atagctatca aactcctaaa gaaataacgt
    10681 acaaatcaat tgtaggtgga aaattgaata atttgtttgt aataggtaaa ggtgcttacc
    10741 aatccgaaag ttttagatca caaattagtt gtatgttaat ggggatatca tcagcagcag
    10801 cggcaaaaac tattattgaa gataaaagca atactctaaa cattaataga gatttgttta
    10861 aatcgaactt agatatatta tttacatggg agttttctaa ttggattact gattaacaaa
    10921 taataaggga atattaaatg aatgatacta ataaaaatca atactttgtt tatgaatttg
    10981 atactagctt tgtagaaatg tcttattctc agttttttga aggatattgt attctttttt
    11041 caaaagaacc ctatccttct ttatctgata tgccaatgga atttagaaaa gaatttttaa
    11101 tggaaatgag tatattaggt gatgcgatgc ttgaagtgtt aaatgcctat agaattaatt
    11161 atgatatctt aggaaattca acacctgtac ttcatgctca tttatttccg agatacatgt
    11221 gggaagagga agagagaaga aaaactgtag ttcatagata tgattcttca gtttttagca
    11281 atcctgagtt tgaatttact atagaaaagc atggaccaat tattaaaaaa ctgaggtctg
    11341 ctattgatag acattataga gagtatcaac aattattaga tgattcaaac tgaactccac
    11401 attttcgatt agatttaata ccttgtatat tacaactagg ttgtggatga tataattttt
    11461 tgaaaaatat attttaggaa tgacttataa agcaattata tcagaggcta ttaaatcaat
    11521 ggccaaagct tatgctcctt attctgattt taaggttgga gctgcgttag taactgaaga
    11581 taaaaaattt tttctggatg taatatagaa aatgctgctt tttctcctac ttgctgtgca
    11641 gaacgagtat ctttttataa agctgtattt aaaggagaac gttcgttttc aagaacatgt
    11701 attgtaggtg gaaaaaatgg tatcttatca tcttttatag caccttgcgg agtgtgtcgc
    11761 caggtaatga tagaatttgt aatcctgatt catttgaagt tatattggct agtgatgttg
    11821 atacttataa agtatataaa cttaaagaac tactaccagt tagttttaat caagaaaatt
    11881 tataacagga gggaactatg gaattagttg aatttaaaaa tggacatgtt aatacggttc
    11941 atgagcatat cgcaataacg aaagtaaaag agtttctaaa taatcatgat atattaaaag
    12001 ataaaggtgg catacataaa atttctactg atttctttta caatataatt gatatggaaa
    12061 ctacaactga agagaataga ccgtgggaaa gtcataagga atattatgat gtccattatc
    12121 tgttaaatgg agaagaaaca attttatata acttcttgtc tcaaatggaa ttgtctgaat
    12181 ataaagtaga tgatgattgg caacaaatga atgggacagc tttatttagt attaaattaa
    12241 agaaagatat gttacttttg ttggaaccaa acgatgcaca tagaacaggc ctattggttg
    12301 aagaaccact taatattaag aaagttgttt ttaaggtaaa aatttaactg aaagtacttt
    12361 cggggattaa gaattttaaa atatttaaga aattgtattc taaagttcta aaattataga
    12421 tatttaattt aaagcttgtt aaaatagaat agtaacactc atctgctttc aaaaagcatt
    12481 ctattccatc tccgattaac gatggacttt atcacctcct tctccagtcc ttgtatgaca
    12541 tcttggagtt gatt
//
LOCUS       DAVBBS010000056        12039 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVBBS010000056 DAVBBS010000000
VERSION     DAVBBS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12039
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.056"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..134
                     /locus_tag="VQU32_001695"
     CDS             <1..134
                     /locus_tag="VQU32_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET2473115.1"
                     /translation="KVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            326..2617
                     /gene="pcrA"
                     /locus_tag="VQU32_001696"
     CDS             326..2617
                     /gene="pcrA"
                     /locus_tag="VQU32_001696"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HET2473116.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTTYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            2660..3340
                     /gene="radC"
                     /locus_tag="VQU32_001697"
     CDS             2660..3340
                     /gene="radC"
                     /locus_tag="VQU32_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET2473117.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3337..4026)
                     /locus_tag="VQU32_001698"
     CDS             complement(3337..4026)
                     /locus_tag="VQU32_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET2473118.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYNLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(4044..4685)
                     /locus_tag="VQU32_001699"
     CDS             complement(4044..4685)
                     /locus_tag="VQU32_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET2473119.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(4768..4983)
                     /locus_tag="VQU32_001700"
     CDS             complement(4768..4983)
                     /locus_tag="VQU32_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET2473120.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(4991..5338)
                     /locus_tag="VQU32_001701"
     CDS             complement(4991..5338)
                     /locus_tag="VQU32_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HET2473121.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFSLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5343..6458)
                     /locus_tag="VQU32_001702"
     CDS             complement(5343..6458)
                     /locus_tag="VQU32_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2473122.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(6468..7427)
                     /locus_tag="VQU32_001703"
     CDS             complement(6468..7427)
                     /locus_tag="VQU32_001703"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET2473123.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(7540..8109)
                     /locus_tag="VQU32_001704"
     CDS             complement(7540..8109)
                     /locus_tag="VQU32_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HET2473124.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAEDYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            8237..8908
                     /locus_tag="VQU32_001705"
     CDS             8237..8908
                     /locus_tag="VQU32_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HET2473125.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            8892..9710
                     /locus_tag="VQU32_001706"
     CDS             8892..9710
                     /locus_tag="VQU32_001706"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HET2473126.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            9707..10603
                     /locus_tag="VQU32_001707"
     CDS             9707..10603
                     /locus_tag="VQU32_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2473127.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            10647..11621
                     /gene="pta"
                     /locus_tag="VQU32_001708"
     CDS             10647..11621
                     /gene="pta"
                     /locus_tag="VQU32_001708"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HET2473128.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
BASE COUNT         3509 a         2300 c         2588 g         3642 t
ORIGIN      
        1 gcaaggttga gaacatcttt gccaaagtaa aaacgtttaa aatgttttca acaacctatc
       61 gaaatcatcg taaacgcttc ggattacgaa tgaatttgat tgctggcatt atcaatcatg
      121 aactaggatt ctagttttgc aggaagtcta ttgaggtatt gagctagttt atgaaaaaat
      181 tgggtgaaaa agtcgagtgt tttagaaacc cacagtgtag tattctagtt tcaatccact
      241 atattttgct actccccgta aagtttctat tttccctgat ttctgatata atagaaatat
      301 tgacttcaag agtaaggaag agaagatgaa cgcattatta aatggaatga atgaccgtca
      361 ggctgaggcg gtgcaaacga cagaaggtcc cttgctaatc atggcaggag ctggttctgg
      421 aaagactcgt gttttaaccc accgtatcgc ttatttgatt gatgaaaagc tggtcaatcc
      481 ttggaatatc ttggccatta cctttaccaa caaggctgcg cgtgagatga aagagcgtgc
      541 ttatagcctc aatccagcta ctcaggactg tctgattgcg accttccact ccatgtgtgt
      601 gcgtattttg cgtcgcgatg cggaccatat tggctacaat cgtaatttta caattgtgga
      661 tcctggtgaa cagcgaacgc tcatgaaacg tattctcaaa cagttgaact tggatcctaa
      721 aaaatggaat gaacgaacta ttttggggac catttccaat gctaagaatg atttgattga
      781 tgatgttgct tatgctgctc aagctggcga tatgtatacg caaattgtgg cccagtgtta
      841 tacaacctat caaaaagaac ttcgtcagtc tgaatccgtt gactttgatg atttgattat
      901 gctgaccttg cgtctctttg atcaaaatcc tgatgttttg acctactacc agcaaaaatt
      961 ccaatacatc cacgttgatg agtaccaaga taccaaccac gctcagtacc aattggtcaa
     1021 actcttggct tcccgtttta aaaatatctg tgtggttggg gatgcggacc agtctatcta
     1081 cggttggcgt ggtgctgata tgcagaatat cttggacttt gaaaaggatt accccaaagc
     1141 taaggttgtt ttgttggagg aaaattaccg ctcaaccaaa accattctcc aagcggccaa
     1201 cgaggttatt aaaaataata aaaatcgccg tcctaaaaat ctctggactc aaaacgctga
     1261 tggggagcaa atcgtttact atcgtgctga tgatgagctg gatgaggctg tatttgtagc
     1321 cagaaccatc gatgaactta gtcgcagtca aaacttcctt cataaggatt ttgcagttct
     1381 ctatcggact aatgcccagt cccgtacaat tgaggaagcc ctgctcaagt ctaacattcc
     1441 ttataccatg gttggcggaa ccaaattcta cagccgtaag gaaattcgcg atattattgc
     1501 ttatctcaac cttattgcta atttgagtga caacattagt tttgagcgta ttatcaacga
     1561 gcctaaacgt ggaattggtc taggtacagt tgagaaaatc cgtgattttg caaatttgca
     1621 aaatatgtct atgctggatg cttctgctaa tattatgttg tctggtatca agggtaaggc
     1681 agcccaatct atctgggatt ttgccaatat gatgcttgat ttgcgggagc agctagacca
     1741 cttaagcatt acagagttgg ttgagtccgt cctagaaaaa acaggttatg tcgatattct
     1801 taacgcccaa gcgactctag aaagcaaggc aagggttgaa aatatcgaag agtttctttc
     1861 tgttacgaag aactttgatg acaccactga tgtgacagaa gaggaaactg gtctggacaa
     1921 actgagtcgt ttcttaaatg acttggcttt gattgccgac acagattcag gtagtcagga
     1981 gacatcagaa gtgaccttga tgaccctgca tgctgccaaa ggtctcgaat ttccagttgt
     2041 ctttttgatt gggatggaag aaaatgtctt tccacttagt cgtgcgactg aagatccaga
     2101 tgaattagaa gaagagcgcc gtctagccta tgtaggtatc acgcgtgcag agaaaattct
     2161 ctatctgacc aatgccaact cacgcttgct ttttggtcgt accaattata accgtccgac
     2221 tcgttttatt aacgaaatca gttcagactt gcttgagtat caaggtctgg ctcgtcctgc
     2281 aaatacaagc tttaaggcat catatagcag tggtagtatt tcctttggtc aaggtatgag
     2341 tttggctcag gctcttcaag accgtaaacg cggtgctgcc ccaaaatcga tccagtcaag
     2401 cggtcttcca tttggtcaat ttacagctgg cgcaaaatca gcatctagcg aggcaaattg
     2461 gtccattggt gacattgctc tccacaagaa atggggagag ggaactgttc tggaagtttc
     2521 aggtagcggt gctaggcagg aattgaaaat caatttccca gaagtaggtt tgaaaaaact
     2581 tttagccagt gtggctccaa ttgagaaaaa aatctaattt tccatccttc tcacgaataa
     2641 taaagtgagg aggattttta tgtacagtat ttcattccaa gaagattcac tattaccaag
     2701 agaaaggctg gccaaggaag gagttgaagc gcttagtaac caagagttgc tagctatttt
     2761 actcaggaca ggaacacgtc aagctagcgt ttttgaaatt gcccaaaaag tcttgaacaa
     2821 tctttcaagc ctaacggatt tgaaaaaaat gaccctgcag gaattgcaga gtttgtctgg
     2881 tattgggcgt gttaaggcca tagaattaca agctatgatt gaactggggc atcgtattca
     2941 caaacacgag actcttgaaa tggaaagtat tctcagcagt caaaagttgg ccaagaagat
     3001 gcagcaggaa ttaggggata aaaaacaaga gcacctggtg gcactctatc tcaatactca
     3061 aaatcaaatc atccatcagc agaccatttt tatcggttct gtaactcgta gtatcgctga
     3121 accgcgagag attcttcact atgcaatcaa gcatatggcg acttctctta tcttggtcca
     3181 caatcatcct tcaggagcgg tagcgcctag ccaaaatgat gatcatgtca ctaaacttgt
     3241 taaagaagcc tgcgaattga tggggattgt tctcttggac catttgattg tctctcattc
     3301 taattacttt agttatcgtg aaaagacaga tttaatctaa agttcattaa cgacatagtc
     3361 aaagagtttt ttatctttgg gacgattttc aaaaagaagt tctggatgcc attggacacc
     3421 gagaaaggcg acatcatccg tactcatgac agcctcaatg ataccatctt taggatcatg
     3481 agccacaact tttaaatttg gtgctaagtc cttgatgctc tggtggtgga aggagttgat
     3541 atgagagatt tctccataga tttcttggag aacggtatct ggttctgtta ccaagcgttg
     3601 agttgtgtac tcaacagaag aatcctgcca atggtcttcg atatcttggt acaaagttcc
     3661 acccatggca acgttaaaga gttgggtacc acggcagaca gagaaaatgg gctttttctg
     3721 tttaatagct tccttgatga gggccagttc gaagatatct ctttgaaggt tatagtcatc
     3781 gctatcaatg gttttgggtt cgccataaaa ttttggatcg acattttgcc cacctgtcaa
     3841 gatgagcttg tcaatcaaac tgatatagtg gcaggccatt tcttgatcac caatcggtag
     3901 gatgatggga atccctccag catctttaac gccttcaaca aagccttttg ctgcgtagct
     3961 catcatgatg tcatcatctg gatgagcttt ttcgtttcct gtaatcccaa taactggttt
     4021 tttcataaaa tgattttcgc tttctaatcc tcttttcgca tgaagtagag gagggtttgg
     4081 agttcacttg tcaaatcgac atactgaacg accacgtctt ttggtaaatg cagatggact
     4141 ggtgaaaaac tgagaattcc tttcacacca gcatcaacca agagattagc aacctcttgt
     4201 gacttgacgc tgggaacagt taggatagca gtcttcacat cagcatcctt gattttatcc
     4261 ttgatctgag aaatcccgta aatgggaatc ccgtcaggag tttgggtacc gacttcagga
     4321 tggtcgtcta ggtcaaaggc catgataatc ttcatcttgt tacgttcgtg gaagcggtag
     4381 tggagaaggg catggcccat atttccaata ccaaccagca tgacattggt aatagagttg
     4441 tcattgagca aatcggcaaa aaatgtcatt agttttttga catcatagcc aaaaccacga
     4501 cgaccaagtt caccaaaata ggaaaaatca cgacgtacgg tcgctgaatc aataccgata
     4561 gcctctgcaa tttgcttaga gttggcacgt tcaatctttt ctgcatgaaa tctcttaaaa
     4621 attcgatagt agagagagag tctttttgct gtagcttttg gaatagcaaa ctgtttatct
     4681 ttcacaaaat cacaaccttt ctattcttct attttataga aacattgtga aaaaatcaac
     4741 aaaaataaga aaaaactaag aaaaatctta gttttgatgt aaaaaatctg catgagatag
     4801 aaaacggtag aggtctccga ccagaccctg ataaactttt tggcccctaa aagtcagaga
     4861 agtcacataa agtgtatctg gtaaggttac acatcctgac aaagtcaaca tgagagcctc
     4921 atgatcctca tacttgagag tacgctctac atgatagcag tccttatagg tcagttcaaa
     4981 cattttggct ctatctttcc gattttgtaa agacaccacg ttctaccaag ctatccatga
     5041 ggaagtagaa tttttcctga tgaatatggt ggtcttctga tttgaaaata tcaactagac
     5101 gaaggctaaa cttgtcagtg atattgattt tagcccctgt aagttccttg ttaatgatga
     5161 ttttgagttg gaagccttca ccgctgtttg gcactttttc caaaaggcga gtcagttcat
     5221 agttaccaac cttagtttca aaaaaggtgt tatctttgag ggtgaatttt ttaacagaag
     5281 ggctaagagt gtaatcgtaa cgacaatttt ttaactgaat gattttttca aatgccatat
     5341 ggctaacctc cgataatttc ttttaaggtt tttgcgaggg tttgtaggtc ttcaacggta
     5401 ttttgtggcg acaaactgat gcgaagggat tccttcaagc gttctgaatt tgcgccatac
     5461 atggcttcaa gaacatggct ggattggaca acgcctgcag tacaggctga gccagtagag
     5521 attgaaattc cagctaaatc tagccgaagg agtaagaggt catttttctg accaggaaat
     5581 ccaatattga gaacataagg gagatgatgt tttcctctat tcaagtaata ctgaatgccc
     5641 tccagctctg ccagaaaggc ggtttctaga ttttgtacat gttgaaaatg ttcttcttgt
     5701 ttttctaggt cttcttttag ggctgcaacc atgcctacga tagcaggcag attttcagtt
     5761 cctgcacgtt ttttctgttc ctggtctccg ccatgtagat aggaatcaaa gtccatgcta
     5821 gatgcgtaga gaaaaccgat tcccttagga ccatggaatt tgtgggcaga agcagtgaga
     5881 aaatcaatgc ccaattcttc tgaatgaatt gggattttac caatagcctg aactgcatca
     5941 acatgatagg cagcagggtg ttgcttgagt atttggccaa tttcagcgat gggcagtagg
     6001 tttcctgtct cattattggc aaacatggta gaaaccaaaa tcgtatcgtc acgtaaagcc
     6061 ttttgaattt gctgggctgt gatttcttga ttttctggct ggataatggt tgcttcaaac
     6121 ccaaagtgtt gaaccaagta atcaattgtt tcaaggacag catggtgctc gatggcagtt
     6181 gtgatgatat gttttccttg ttcttggtga cgaagacagt agccaatgat ggtagtatta
     6241 ttgccttcag tcccaccaga agtgaaaaag atatgttgag gttttgtcct tagtaactgg
     6301 gctagttcct gacgggcttc tcgcaagagt ttgccagctt gacgaccatg accatgaata
     6361 ctagaaggat ttccgtgggt ttcttgcata accttggtca tagctgaaat agcaactgct
     6421 gacataggag tcgttgcagc attgtccaaa taaatcaaag aatcacctta tttcttttta
     6481 ttgtaggcaa agagtgggct gactggtttt ctttcgtgaa tacggacgat agcatcacca
     6541 attaactcac tagcagtgat gtagcataca tttttaggag ttttttcttt tgttgctact
     6601 gaatcagtca caagaatttc tttaatatta gtattgtcaa gaagctcagc agctccctcg
     6661 acgaagagac cgtggctaga aacagcataa atttctgtag ctccttcacg ttcaacgatt
     6721 ttagaagctt cagagaaggt acgtcctgta tttaaaatat catcaatcaa gatagctttc
     6781 ttaccttcaa catcaccaat aatataacct tcgttacgag ttgcatcgtc ttgagggtag
     6841 tcgataatgg cgataggagc atcaagatat tcagccaggc tacgcgcacg tttgacacct
     6901 gaatttttag ggctaacgac aacaacatct gaaccaagca atcctttatc gcagtaatgt
     6961 tttgcgaata ggggaacagt gaaaagatta tccactggaa tatcaaagaa accttgaacc
     7021 tgaacggcat gcaaatcaag agtcaggata cgatcaactc cagccttaac cagcatattg
     7081 gcaactagtt ttgctgtaag tggctcacga ggacaagcaa tgcggtcttg acgtgcatag
     7141 ccaaaatatg gaaggacaac gttgatactg tgggcacttg cacgcacaca agcatcgacc
     7201 atgattaaca attccattag gtggttgttg acagggaaac ttgttgattg gatgatgtaa
     7261 acatcataac cacggacact ttcttcgata tttacttgga tttctccgtc tgaaaattga
     7321 cgtgatgata gttttccaag tgggacacca acagcttggg caattttttg tgcaatctct
     7381 tggttagagt tgagtgcgaa aagtttcatg ttttttctat ctgacattat agaccgtcct
     7441 ctgtaaactt tataaatcct agttatattt accttacata tatgaactgg gatttgtgta
     7501 tttttatctt ttctatttta ccaaaaaatg gagattattt cagctatttt tcatactttt
     7561 gacaaatcga accaattttg aaggagcttt ttggtaggaa atctgatttt tctctaaaaa
     7621 ttgtcggaaa tcctgtttgc cttgctcatg attttccact tcaagctcca attcgtaatc
     7681 tgttatatca aagtatcggc tctgatccag tgccatgaga ccaatagctg ttttcatttc
     7741 atagcgaagc gttgttagac aaccaagaac ctgccagttc ttactttgga taccatgttt
     7801 cgccaattca tccagtacta gtccttgagg aagttcttcc ttactcagat agtcctcagc
     7861 atcttttagt tgcaattttt ggttgtattc catgtttcca acactctgcg ggactttgag
     7921 tgtcaactca gcccagtctt caaaggttcg aatgcgcata gcgactttct tttctcgcag
     7981 ttcaaaatca ggcgtgtcga tgtagtaatt tgtttgaaga acaggagtga cacctgtgaa
     8041 ctggtctttt agacgattgt attcatcttt tttcaatagt gttttcaatt caatttctaa
     8101 atgtttcatt tttcttacct ttttttatcg ttgaaagcgg atttatggta taataagcat
     8161 tgtatttatt gtatatgaat ctggagaaaa aatcaaagat atttttgacg gataatatga
     8221 gaacaaggga gaatatatga ccttagaatg ggaagaattt ctagatcctt acattcaagc
     8281 tgttggtgag ttaaagatta aacttcgtgg tattcgtaag caatatcgta agcaaaataa
     8341 gcattctcca attgagtttg tgaccggtcg agtcaagcca attgagagca tcaaagaaaa
     8401 aatggctcgt cgtggcatta cttatgcgac cttggaacac gatttgcagg atattgctgg
     8461 cttacgtgtg atggttcagt ttgtagatga cgtcaaggaa gtagtggata ttttgcacaa
     8521 gcgtcaggat atgcgaatca tacaggagcg agattacatt actcatagaa aagcatcagg
     8581 ctatcgttcc tatcatgtgg tagtagaata tacggttgat accatcaatg gagctaagac
     8641 tattttggca gaaattcaaa ttcgtacttt ggccatgaat ttctgggcaa cgatagaaca
     8701 ttctctcaac tacaagtacc aaggggattt cccagatgag attaagaagc gactggaaat
     8761 tacagctaga atcgcccatc agttggatga agaaatgggt gaaattcgtg atgatatcca
     8821 agaagcccag gcactttttg atcctttgag tagaaaatta aatgacggtg taggaaacag
     8881 tgacgataca gatgaagaat acaggtaaac gaattgatct gatagccaat agaaaaccgc
     8941 agagtcaaag ggttttgtat gaattgcgag atcgtttgaa gagaaatcag tttatactca
     9001 atgataccaa tccggatatt gtcatttcca ttggcgggga tggtatgctc ttgtcggcct
     9061 ttcataagta cgaaaatcag cttgacaagg tccgctttat cggtcttcat actggacatt
     9121 tgggcttcta tacagattat cgtgattttg agttggacaa gctagtgact aatttgcagc
     9181 tagatactgg ggcaagggtt tcttaccctg ttctgaatgt gaaggtcttt cttgaaaatg
     9241 gtgaagttaa gattttcaga gcactcaacg aagccagcat ccgcaggtct gatcgaacca
     9301 tggtggcaga tattgtaata aatggtgttc cctttgaacg ttttcgtgga gacgggctaa
     9361 cagtttcgac accgactggt agtactgcct ataacaagtc tcttggcggt gctgttttac
     9421 accctaccat tgaagctttg caattaacgg aaattgccag ccttaataat cgtgtttatc
     9481 gaacactggg ctcttccatt attgtgccta agaaggataa gattgaactt attccaacaa
     9541 gaaacgatta tcatactatt tcggttgaca atagcgttta ttctttccgt aatattgagc
     9601 gtattgagta tcaaatcgac catcataaga ttcactttgt cgcgactcct agccatacca
     9661 gtttctggaa ccgtgttaag gatgccttta tcggtgaggt ggatgaatga ggtttgaatt
     9721 tatcgcagat gaacatgtca aggttaagac ctttttaaaa aagcacgagg tttctaaggg
     9781 attgctggct aagattaagt ttcgaggtgg agctattctg gtcaataatc aaccgcaaaa
     9841 tgcaacgtat ctattggacg ttggagacta cgttaccatt gacattcccg ctgaggaagg
     9901 ctttgaaacc ttggaggcta ttgagcatcc attagatatt ctctatgagg atgaccactt
     9961 tctagtcttg aataaaccct atggagtggc ttctattcct agtgtcaatc actctaacac
    10021 cattgccaat tttatcaagg gttactatgt caagcaaaat tatgaaaatc agcaggttca
    10081 cattgttacc agactagata gggacacttc tggcttgatg ctctttgcca agcacggtta
    10141 tgcccatgca cgattagaca agcagttgca gaagaaatct atcgagaaac gctactttgc
    10201 tttggttaag ggagatggac atttggagcc agaaggggaa attattgctc cgattgcgcg
    10261 tgatgaagat tccattatta ccagacgagt ggctaaaggc ggaaagtatg cccatacttc
    10321 atacaagatt gtagcttctt atggaaatat tcacttggtc tatattcacc tgcacactgg
    10381 tcgaacccat caaatccgag tccatttttc tcatatcggt tttcctttgc tgggagatga
    10441 tttgtatggt ggtagtctgg aagatggtat tcaacgtcag gctctgcatt gccattacct
    10501 atccttttat catccatttt tagagcaaga cttgcagtta gaaagtccct tgccggatga
    10561 tttcagtaac cttattaccc agttatcaac taatactcta taaaaactgt ctcagagtat
    10621 aattattatc ttaaaggaga aaactcatgg aagtttttga aagtctcaaa gccaaccttg
    10681 ttggtaaaaa tgctcgtatc gttctccctg aaggggaaga gcctcgtatt cttcaagcaa
    10741 caaaacgctt agtaaaagaa acagaagtga ttcctgtttt gcttggaaat cctgaaaaaa
    10801 ttaaaattta tcttgaaatt gaaggaatca tggatggtta tgaggtcatc gaccctcaac
    10861 attatcctca atttgaagaa atggtttctg ccttggtgga gcgtcgcaag ggcaaaatga
    10921 ctgaagaaga tgtacgcaag gttttggttg aagatgtcaa ctactttggt gtgatgttgg
    10981 tttacttggg cttggttgat ggaatggtgt caggagcgat tcactcaaca gcttcaacag
    11041 ttcgtccagc tctacaaatc atcaaaactc gtccaaatgt aactcgtact tcaggagcct
    11101 tcctcatggt tcgtggtacg gaacgttacc tatttggaga ctgtgccatt aacatcaatc
    11161 cagatgcaga agccttggct gaaattgcca tcaactcagc aatcacagct aagatgtttg
    11221 gcatcgaacc taaaattgcc atgttgagct attctactaa aggttcaggg tttggtgaaa
    11281 gcgttgataa ggtcgttgaa gcaactaaaa ttgctcacga cttgcgtcct gaccttgaaa
    11341 tcgatggtga gttgcaattt gatgcagcct ttgttcctga aactgcagct ctgaaagctc
    11401 ctggaagtac ggtagctggt caagcaaatg tcttcatctt cccaggtatc gaggccggaa
    11461 atatcggtta caagatggct gaacgcctgg gtggctttgc ggctgtagga cctgttttgc
    11521 aaggtttaaa caagccagtt aatgatcttt ctcgtggatg taatgcagat gatgtttaca
    11581 agttgaccct catcacagca gctcaagcag ttcatcaata gtgaaaacta taaagcgata
    11641 tactatgcta tactgtagtt atgaaactat gtacgaaaag cactgccatt aattcctgag
    11701 aactaaatta ctgattggtg tcaaaaagga aaacttccaa gcgatgatat cctgtctata
    11761 cacgacctat agaaatctgt aatatgtatg tccgtaaaac gataatttcc ctttttgatt
    11821 ttaaatgagt atgaaaagag aattttttca tgagaaaacg cataatatta agtgtcttga
    11881 cacctgctat tatgcgtttt ctggctcttt gtcaactgta gtgggttgaa gaaaagctaa
    11941 gctcgagaaa ggacaaattt tgtcctttct tttttgatat tcagagcggt aaaaatccgt
    12001 tttttgaagt tttcaaagtt ccgaaaacca aaggcattg
//
LOCUS       DAVBBS010000057        11557 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVBBS010000057 DAVBBS010000000
VERSION     DAVBBS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11557
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.057"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            184..438
                     /gene="blpM"
                     /locus_tag="VQU32_001709"
     CDS             184..438
                     /gene="blpM"
                     /locus_tag="VQU32_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HET2473129.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            454..657
                     /gene="blpN"
                     /locus_tag="VQU32_001710"
     CDS             454..657
                     /gene="blpN"
                     /locus_tag="VQU32_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HET2473130.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            901..1050
                     /gene="blpO"
                     /locus_tag="VQU32_001711"
     CDS             901..1050
                     /gene="blpO"
                     /locus_tag="VQU32_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HET2473131.1"
                     /translation="MDTKMMSQFAVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            1154..1273
                     /locus_tag="VQU32_001712"
     CDS             1154..1273
                     /locus_tag="VQU32_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HET2473132.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            2059..2457
                     /locus_tag="VQU32_001713"
     CDS             2059..2457
                     /locus_tag="VQU32_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HET2473133.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEVGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKKQTVFLILQQ"
     gene            2495..3184
                     /locus_tag="VQU32_001714"
     CDS             2495..3184
                     /locus_tag="VQU32_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2473134.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNFV
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            3226..3474
                     /locus_tag="VQU32_001715"
     CDS             3226..3474
                     /locus_tag="VQU32_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473135.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLKWFVLERLELKDDA"
     gene            3504..4115
                     /locus_tag="VQU32_001716"
     CDS             3504..4115
                     /locus_tag="VQU32_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2473136.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            4276..5070
                     /locus_tag="VQU32_001717"
     CDS             4276..5070
                     /locus_tag="VQU32_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET2473137.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            5067..5702
                     /gene="trmB"
                     /locus_tag="VQU32_001718"
     CDS             5067..5702
                     /gene="trmB"
                     /locus_tag="VQU32_001718"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HET2473138.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            5828..6307
                     /gene="rimP"
                     /locus_tag="VQU32_001719"
     CDS             5828..6307
                     /gene="rimP"
                     /locus_tag="VQU32_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET2473139.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            6351..7487
                     /gene="nusA"
                     /locus_tag="VQU32_001720"
     CDS             6351..7487
                     /gene="nusA"
                     /locus_tag="VQU32_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HET2473140.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            7509..7802
                     /locus_tag="VQU32_001721"
     CDS             7509..7802
                     /locus_tag="VQU32_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HET2473141.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            7795..8094
                     /locus_tag="VQU32_001722"
     CDS             7795..8094
                     /locus_tag="VQU32_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HET2473142.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            8111..10903
                     /gene="infB"
                     /locus_tag="VQU32_001723"
     CDS             8111..10903
                     /gene="infB"
                     /locus_tag="VQU32_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET2473143.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            11154..11504
                     /gene="rbfA"
                     /locus_tag="VQU32_001724"
     CDS             11154..11504
                     /gene="rbfA"
                     /locus_tag="VQU32_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET2473144.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         3527 a         2022 c         2631 g         3377 t
ORIGIN      
        1 tgctggtatt atcaatcatg aactaggatt ctagttttgc aggaagtcta atgtaatttt
       61 tgcaattcaa gacgtttcga tgactattca agatctggat gaaaaatttt aaaaaacgat
      121 gatatactaa acttgtcaaa gttacaacaa gacaaaaatt aaaaataaaa aagagtattt
      181 gtcatggata caaaaattat ggaacaattt catgaaatgg atataactat gttatctagt
      241 attgaaggag gcaagaataa ttggcaaact aatgtcttgg aaggtggtgg tgctgctttt
      301 ggtggctggg ggctagggac agctatttgt gctgcgagtg gtgttggagc accatttatg
      361 ggagcatgtg gatacatagg agctaaattt ggtgtggatc tttgggcagg agtaacaggg
      421 gcaacgggtg gattttaata aaaggagact tgtatgaata cttattgtaa tataaatgaa
      481 acaatgctga gtgaggttta tggaggtaat tccggaggag cagctgtagt tgctgcttta
      541 ggttgtgcag cgggtggagt gaaatatggg agacttctag gaccatgggg cgctgcaata
      601 ggaggaattg gaggagcagt ggtttgtgga tatttagcct ataccgctac atcataaaaa
      661 tgaaagctaa attttatgaa ggctatattc tttattattc tcttcgcttt tcaaacctat
      721 cttatttatc tatccataag tatctccgat aaaaaacaaa agactattga attaacatca
      781 ttgaattgtt ttgtcatatt atttctaatc tatgataaat taatttttct ctttattgcc
      841 tatgtttttt tgataatttt tatattaaac ttgttccgta actgaaaagt gatatactat
      901 atggatacaa aaatgatgtc acaatttgca gttatggata atgaaatgct tgcttgcgtt
      961 gaaggtggag atattgattg gggaagaaaa attagttgtg cagcaggggt tgcatatggc
     1021 gcaattgatg ggtgtgcaac aacggtttga catttctatt gggaccattt gctataggaa
     1081 taggtgtaac tggtgctgca ggtggagcta tacttggagg tgtagcctat gcagcaatat
     1141 gttggtggta attatggatt ttaaaagttt tattattggt ttagtagttg gtatatttgg
     1201 tccttatatg gatgacttaa ttagaaaaat attttcaaaa tcttccaaaa aggatacgga
     1261 tagtactctc taaaaaataa atttctcccc tgtatttgct agcttaattt acaggggagt
     1321 ttctttaata ttgtttcgcc tgtcaaaatg atatgtgata gcaggatgaa aagtctacaa
     1381 acaagttggt atcaatacaa tataaagagg aaagtatgtt tttaatgctt actttttaaa
     1441 ttttttaaca attcaagatg tttcgatgac aattcaagat ttggatgaaa aattttaaaa
     1501 aacaatgata tactaaactt gtcaaagttg caacgggtgc tgctacatta tgttaaaaat
     1561 cgctcttgat tgataagctg gatttgttag gaaaggagta aataattatg gataagaaaa
     1621 agatagtttc gactataata tgtatagtat ttcttgtagt gtcagtagat aattttttta
     1681 gagacttaac cccgttactg ttcattttaa atattattgg cctgtcttgc ttttcagtgc
     1741 taacatacat taacataaaa gagatactct taaatataag taaatgatga tttggttagt
     1801 cctagaacca atcaacttat gtttatcggc ttggcagatt ttatgtttgt catttgtttg
     1861 tatagaggta tttcagaaac agaattctat caacaactaa tcgcttatat aggtgtattc
     1921 tctgcttgtt tatcacgctt ctgctcatgt ggggcttgaa gtattataaa aagtagcttt
     1981 ctatcctttt ctgacaggat atttgagtag aaggctctat taaattgtta gtgaaataaa
     2041 tgaatggagg tatttaatat gaaatacaga ttattttttg ttattttctt gagtagtgtg
     2101 ttggatattc ttttagggac atttttacaa atctctatcg tatctattgg gtggcttgtt
     2161 ctttacagtg gtttgtttga agtaggagtt ttccttcttg ctaataaagg ggtggcggta
     2221 aaaatcaagg aagtagatat tcgaaatcgc tttaaattta tttttggaaa aaccttatgg
     2281 tttcaaattc ttttgctcat ctttttgata atcaaacttt atcttggttt ggatgcgagg
     2341 ttgattttat tctatggaca tattttcatt gtctttaatg ccttaatgta tctattatct
     2401 agtagtcagg ttagccttaa aaaaaaacaa actgtcttct taatcttaca gcagtagagt
     2461 ctgacaaggt gaggtcatag tagggaggat ttctatgaaa aagtatcaac ttctattcaa
     2521 aataagtgca gtcttctctt acttattttt cgtatttggt ctttctcagc tgacgcttat
     2581 cgtccaaaat tattggcaat tttcttccca gattggtaat ttcgtctgga ttcaaaatat
     2641 cttgagttta ctttttattg gagtcatgat tgtggttctt gttaagacag gccatggtta
     2701 tctcttccgc attccaagaa aaaaatggct ttggtattcg attttgacag tattagtggt
     2761 agtgttccag atctctttta acgttcagac agctaaacat gttcagtcaa ctgcggaagg
     2821 ttgggctgta ttgattggtt atagtgggac taactttgca gagctaggta tttatatagc
     2881 cctgttcttt ctggttccac tgatggaaga attgatttat agaggattac tgcaacatgc
     2941 tttctttaag cattcgcgat ttggtcttga tttgcttctt ccttctattt tatttgctct
     3001 ccctcatttt tcaagcctgc ctagtctgtt agatatcttc gtctttgcaa cagttggaat
     3061 catctttgct ggtttgaccc gctataccaa gagcatttat ccatcctatg cggtgcatgt
     3121 gatcaataat attgtagcga ccttcccgtt tttgctcact tttctacata gggtcttggg
     3181 gtaaaaaaac aaaaggcttg cttttcagcc atagaggagg tcatcatgta taaacactta
     3241 tttttcctag attccaaaac tttagatcgg ttgacaccct atattctagt cttggcttct
     3301 gacaccattg cttttaatgt ttttgtgcta acctttgtat ctgcggtggt ttttaatttc
     3361 ctaaattcca tgctagcttt aatggctata ttcatagggg ctggctatgt ggtcggattt
     3421 tggttgctaa aatggtttgt tttggaaagg ttagagctaa aggatgacgc gtaggaagtc
     3481 tgtttggtga ggaggatagt tttatggagt tttttgataa atttcatgcc ttgtgttttg
     3541 gatttttagt actaataatt gtcattacag ttccttatac gattaaccat gggggttttt
     3601 ttcaaaatga atctgcattg attcttgtaa gtcttcttgt aacctcgctg agtgttgctt
     3661 atgctagaaa gtttgaaatg atttcttttg ggatgttaag caagaaacaa cttttgcttt
     3721 tcattgcaat ctttcttcta agtgtacttg agacgctggt ttatattcat ttcttcgctg
     3781 tttcttctgg ctcaggggtc caacacttgg cggaagtcag cagaggaatt tccctgtctt
     3841 tgattttgac tacctcagtt tttggcccca tccaggagga actcattttc agaggacttc
     3901 ttcaaggtgc ggtttttgac aattcttggt tagggcttgt gctaacttcc tctctctttt
     3961 ctttcatgca tggaccttct aatgtccctt cgtttatttt ttatctactt gggggtttgt
     4021 tgctgggctt tgcttataaa aagagtcaaa acctatgggt ttctactcta gtacacatgt
     4081 tttacaacag ttggccactc ctatattatt tataaaaatt atgaaaaggt aagcagaaga
     4141 cggtgcttac ctttttcaag ctataatgat acccaagcca atccagaggc ttttctttga
     4201 gaatcgggtt agagtggttg acgaataggc caaaaactag tagaatagta aggaaacttt
     4261 atacggagga aagaaatgga tttgggtgat aatgagctaa cactgactcc catacctggg
     4321 aaaagtggca aggcttatat gggtagctat cctgacggga agcgcatctt tgtaaaaatg
     4381 aacacctctc caatcctacc tggtctagct agagaacaaa ttgctccaca attattatgg
     4441 agtcgtcgtt tggcagatgg gcgtgatatg tgtgctcaag aatggttgac aggcaagata
     4501 ttgaccccct atgatatgaa tcgtaagcaa atcgtcaata ttttaacccg tcttcatcgc
     4561 tcacgtccgt tgatgacaca attgagtcgt ttgggctatg ccatggaaac acctgtagat
     4621 ttactacagt cttggcagga aacggctcca gatgctttgc gtaaaaatca ttttatcagt
     4681 gaagtgatgg ctgatttacg tcagactatt ccaggattta gagaggacca tgcgaccatt
     4741 gtccatggag atgtacgaca tagtaattgg attgagacag atagtggctt gatttattta
     4801 gtagattggg attcggttcg cttgaccgat cgcatgtttg atgtggccca tatgctctgc
     4861 cattatattt cagaacatca gtggaaggaa tggttgacct actacggtta caagtacaat
     4921 caaacggtat taagtaaatt gtattggtat ggtcaattgt cttatttgag tcagatttcc
     4981 aagtattata tgaaccaaga tttagaaaat gtcaatcggg agattcatgg tttgcgtcat
     5041 ttccgagaca agtatggaaa gagaagatga gagttagaaa tcgtaaaggg gcaacagaat
     5101 tactagaggc aaatccccag tatgtggtcc tcaatccctt ggaagccaag gcaaaatggc
     5161 gggacttgtt tggcaatgat aatcccattc atgtggaagt tggaagtgga aagggtgcct
     5221 ttgtttcagg tatggccaag caaaaccctg acatcaacta tatcgggatt gatattcaaa
     5281 agtctgtttt gagctacgct ttggacaagg tgcttgaagt tggagtgcct aacatcaagc
     5341 tcttgtgggt agatggttct gacttaactg actattttga agacggtgag attgatcgct
     5401 tgtatctgaa cttttcagat ccatggccga aaaaacgcca tgaaaagcgt cgtttgacct
     5461 acaagacctt cttggatacc ttcaaacgta tcttgcctga aaatggagaa attcatttca
     5521 agacggataa ccgtggcttg tttgagtaca gtttagtgag cttttctcaa tatggcatga
     5581 aactcaatgg tgtctggtta gatttgcatg ccagtgattt tgaaggcaat gtcatgacag
     5641 aatacgagca aaaattctca aacaaggggc aagttatcta ccgagttgag gcagaatttt
     5701 aagagatagc ctaaaattag gctgtacaag tgcttttgct ttacataagt tggcaaacgt
     5761 gctatactga tagtaagaat atgaaaagtg aggcggggaa atatcttcgc ctcttgctta
     5821 tgaggaggtg gacgcaatcg caacaatcgt agaattagtc agagaagttg tagaacctgt
     5881 catagaagct ccttttgaac tcgtggatat cgagtatgga aagattggca gtgacatgat
     5941 tctcagtatt tttgtagata aacccgaagg aattaccttg aacgacacgg cagacttgac
     6001 agaaattatc agtcctgtcc tagacaccat caagccagat cccttcccag aacaatattt
     6061 cctagaaatt accagtccag gtttggaacg ccctttgaaa accaaggatg ccgtcgctgg
     6121 agcggttgga aaatacatcc atgtcgggct ctaccaagcc atcgataagc aaaaggtctt
     6181 tgaaggaacc ttgttggcct tcgaagagga cgagttgact atggaatata tggacaagac
     6241 gcgtaagaaa accgtccaaa ttccatacag tttagtatca aaagcacgtt tagcagttaa
     6301 attatagaaa aagaaaggat agcttttgag gattcaaaag tgaagaaaac atgagtaaag
     6361 aaatgctaga ggccttccgc attttggaag aagacaaggg aatcaaaaaa gaagatatca
     6421 tcgacgcagt agtagagtcg cttcgttccg cttatcgcag acgctatggt cagtcagaca
     6481 gcgtagctat tgacttcaac gaaaaaacag gtgactttac agtttatact gtccgtgaag
     6541 ttgttgatga agtatttgat agccgtttgg aaatcagctt gaaagatgct cttgccatta
     6601 attcagctta tgaacttgga gacaaaatca agtttgaaga agcaccagct gagtttggtc
     6661 gtgtagcagc ccagtctgcc aaacaaacca tcatggaaaa aatgcgcaag caaacacgtg
     6721 ccatcactta caatacttac aaagaacatg agcaagaaat catgtctggt acagtagaac
     6781 gctttgacaa ccgctttatc tatgtcaacc ttggtagcat cgaagcccaa ttgtcaaaac
     6841 aagaccaaat tcctggagaa gtttttgctt ctcatgatcg tatcgaagtt tatgtttaca
     6901 aggttgaaga caaccctcgt ggtgtgaacg tctttgttag ccgtagtcat ccagaaatga
     6961 tcaaacgttt aatggagcaa gaaattccag aagtttatga tggaactgtt gaaatcatga
     7021 gcgtggctcg tgaagcaggt gaccgtacga aggttgctgt tcgtagccac aatccaaacg
     7081 tggatgctat cggtacaatc gttggacgtg gtggtgctaa tatcaagaag attactagca
     7141 aattccaccc agctcgttac gatgctaaaa atgaccgcat ggtaccaatc gaagaaaata
     7201 tcgatgttat cgagtgggta gcagatccag ctgaatttat ctacaatgcc atcgctcctg
     7261 ctgaggttga ccaagttatc tttgatgaaa acgacagcaa acgtgccttg gtggttgttc
     7321 cagataacaa gctttctctt gccattggtc gtcgtggaca aaacgtgcgc ttggcggctc
     7381 acttgactgg ttaccgtatc gatatcaagt ctgctagcga atttgaagcc atggaagacg
     7441 ctgcttcagt agagttggaa gtagaaaacg atactgtaga agaataaaag ctgctagagg
     7501 agggaaagat gaaaacaaga aaaatccctt tgcgcaagtc tgttgtgtct aacgaagtca
     7561 ttgataagcg tgatttgctc cgcattgtca agaacaagga aggacaagtc tttattgatc
     7621 ctacgggcaa ggccaatggc cgcggcgctt atatcaaact agacaatgca gaagccctag
     7681 aggcgaaaaa gaagaaggtc tttaaccgca gctttagcat ggaagtggaa gaaagctttt
     7741 atgacgagtt gatcgcttat gtggatcaca aagtgaaaag aagagagttg ggacttgaat
     7801 aagcaaaaga taagtaatct cttggggctt gctcagcgag cagggcgcat catatcgggt
     7861 gaagaattgg tggtcaaggc cattcaagac ggcaaggcca agttggtctt tctagctcat
     7921 gatgctggac ccaatctgac caagaagatt caagataaaa gtcattatta tcaagtagaa
     7981 attgtaaccg tgttttcaac actggaatta agcatagcag tcgggaaatc gagaaaggtt
     8041 ttggctgtaa cagatgctgg atttacaaag aaaatgaggt ctcttatgga atagaagagg
     8101 aggacatgat ttgtctaaga aaagattgta cgaaatcgca aaagaacttg gaaaagaaag
     8161 taaagaagtt gtagcgcgtg caaaagagtt gggcttggat gtgaaaagcc actcatcaag
     8221 tgtggaagaa gctgtcgctg caaaaattgc tgccagcttt aagcctgcag ctgctccgaa
     8281 agtagaagca aaacctgcag caccaaaagt aagtgcagaa aagaaagccg aaaaatctga
     8341 gccagctaaa ccagctgtag ctaaggaaga ggcaaaaccg gctgagccag ttgctccgaa
     8401 aacagaaaaa gtagcagtga aaccgcaaag tcgtaatttc aaggctgagc gtgaagcccg
     8461 tgctaaagag caggcagaac gacgcaagca aaataagggc aataaccgtg accaacaaca
     8521 aaacggaaac cgtcagaaaa acgacggccg taatggtgga aaacaaggtc aaagcaaccg
     8581 cgacaatcgt cgctttaatg accaagctaa gaagcagcaa ggtcagcaaa aacgtagaaa
     8641 tgagcgccgt cagcaagagg ataaacgttc aaatcaagcg gctccacgta ttgactttaa
     8701 agcccgtgca gcagccctaa aagcagagca aaatgcagag tacgctcgtt caagtgagga
     8761 acgcttcaag cagtatcagg ctgctaaaga agccttggct caagctaaca aacgcaagga
     8821 accagaggaa atctttgaag aagcggctaa gttagctgaa caagcacagc aagttcaagc
     8881 agtggttgaa gtcgtccctg agaaaaaaga acctgcagtg gatacacgtc gtaaaaaaca
     8941 agctcgacca gacaaaaatc gtgacgatta tgatcatgaa gaagatggtc ctagaaaaca
     9001 acaaaagaat cgaagtagtc aaaatcaagt gagaaatcaa aagaatagta actggaataa
     9061 caacaaaaag aacaaaaaag gcaataacaa gaacaaccgt aatcagactc caaaacctgt
     9121 tacggagcgt aaattccatg aattgccaac agaatttgaa tatacagatg gtatgaccgt
     9181 tgcggaaatc gcaaaacgta tcaaacgtga accagctgaa atcgttaaga aacttttcat
     9241 gatgggtgtc atggccacac aaaaccaatc cttggatggg gaaacaattg aactcctcat
     9301 ggtggattac ggtatcgaag ccaaacaaaa ggttgaagtg gataatgctg acatcgaacg
     9361 tttctttgtc gaagatggtt atctcaatga agatgaattg gttgagcgtc caccagttgt
     9421 tactatcatg ggacacgttg accacggtaa aacaaccctt ttggatactc ttcgtaactc
     9481 acgtgttgcg acaggtgaag caggtggtat tactcagcat atcggtgcct accaaatcgt
     9541 ggaaaatggt aagaagatta ccttccttga tacaccagga cacgcggcct ttacatcaat
     9601 gcgtgcgcgt ggtgcttctg ttaccgatat tacgatcttg gtcgtagcgg cagatgacgg
     9661 ggttatgcct cagactattg aagccatcaa ccactcaaaa gcagctaacg ttccaatcat
     9721 cgtagccatt aacaagattg ataaaccagg tgctaaccca gaacgcgtta tcggtgaatt
     9781 ggcagagcat ggtgtgatgt caaccgcttg gggtggagat tctgaatttg ttgaaatctc
     9841 agctaaattc aaccaaaata tcgaagaatt gttggaaaca gtccttcttg tagctgaaat
     9901 ccaagaactc aaggcagacc caacagtgcg tgcaatcggt acggttatcg aagcgcgctt
     9961 ggataaagga aaaggtgcgg tcgcaaccct tcttgtacaa caaggtacct tgaatgttca
    10021 agacccaatc gttgtcggaa ataccttcgg tcgtgtccgt gctatgacca acgaccttgg
    10081 tcgtcgtgtt aaagttgctg gaccatcaac accagtctct atcacaggtt tgaacgaagc
    10141 accgatggcg ggtgaccact ttgccgttta cgaggatgaa aaatctgcgc gtgcagcagg
    10201 tgaagagcgt gccaaacgtg ccctcatgaa acaacgtcaa gctacccaac gtgttagcct
    10261 tgaaaacctc tttgataccc ttaaagctgg ggaactcaaa tctgttaatg ttatcatcaa
    10321 ggctgatgta caaggttctg ttgaagccct ttctgcctca cttcaaaaga ttgacgtgga
    10381 aggtgtcaaa gtgactatcg tccactcagc ggtcggtgct atcaacgaat cagacgtgac
    10441 ccttgccgaa gcttcaaatg cctttatcgt tggtttcaac gtacgcccta caccacaagc
    10501 tcgtcaacaa gcagaagctg acgatgtgga aatccgtctt cacagcatta tctacaaggt
    10561 tatcgaagag atggaagaag ctatgaaagg gatgcttgat ccagaatttg aagaaaaagt
    10621 tattggtgaa gcggttatcc gtgaaacctt caaggtgtct aaagtgggaa ctatcggtgg
    10681 atttatggtt atcaacggta aggttgcccg tgactctaaa gtccgtgtta tccgtgatgg
    10741 tgtcgttatc tatgatggtg aactcgcaag cttgaaacac tataaagacg acgttaagga
    10801 agttacaaac ggccgtgaag gtggattgat gatcgacggc tacaatgata ttaagatgga
    10861 tgatgtgatt gaggcgtatg tcatggaaga aatcaagaga taagattttt tgctcctttc
    10921 ttaggtggtg agggacgcaa gcaaaccgat ggtttcattg cttatttttg agcctagggt
    10981 ctcaaaaatc ccctgtgatg ggactgataa atcagttcca tcactttcac cacggcgaaa
    11041 gaagcagatg acttcaaatt gaacttcgtt tcaatttaaa ctgaaaatca agaagtttaa
    11101 aatagctagg tctgctggcc tagcttttgg ttcaaagtag agaaaggaat atcatggcaa
    11161 atcatttccg tacagatcgt gtgggcatgg aaatcaagcg tgaagtcaat gagattttgc
    11221 aaaagaaagt ccgtgatcca cgtgtccaag gtgtgaccat cacagatgtt cagatgctgg
    11281 gtgacttgtc tgttgccaag gtttattaca ccattttgag taaccttgct tcggataacc
    11341 aaaaagccca aatcgggctt gaaaaagcaa ctggtaccat caaacgtgaa cttggtcgca
    11401 atttgaaatt gtacaaaatc ccagatttga ccttcgtcaa agacgagtcc atcgagtatg
    11461 gaaacaagat tgacgagatg ctacgcaatc tggataagaa ctaaagaaga ggggttgccc
    11521 ctctttttcg gctctttgtc aactgtagtg ggttgaa
//
LOCUS       DAVBBS010000058        11394 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVBBS010000058 DAVBBS010000000
VERSION     DAVBBS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11394
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.058"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(7..1221)
                     /locus_tag="VQU32_001725"
     CDS             complement(7..1221)
                     /locus_tag="VQU32_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2473145.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(1377..1670)
                     /locus_tag="VQU32_001726"
     CDS             complement(1377..1670)
                     /locus_tag="VQU32_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473146.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVKVIMICQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            1832..2284
                     /locus_tag="VQU32_001727"
     CDS             1832..2284
                     /locus_tag="VQU32_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET2473147.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(2319..3707)
                     /locus_tag="VQU32_001728"
     CDS             complement(2319..3707)
                     /locus_tag="VQU32_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2473148.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            3773..4735
                     /locus_tag="VQU32_001729"
     CDS             3773..4735
                     /locus_tag="VQU32_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET2473149.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(4732..4923)
                     /locus_tag="VQU32_001730"
     CDS             complement(4732..4923)
                     /locus_tag="VQU32_001730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473150.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDETDEVSSKHVFEVVDRTDEVS
                     NHTYGRATLTWFAEIFEEY"
     gene            complement(4987..5997)
                     /gene="ccpA"
                     /locus_tag="VQU32_001731"
     CDS             complement(4987..5997)
                     /gene="ccpA"
                     /locus_tag="VQU32_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HET2473151.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKVGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(6422..7021)
                     /locus_tag="VQU32_001732"
     CDS             complement(6422..7021)
                     /locus_tag="VQU32_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2473152.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(7022..8119)
                     /locus_tag="VQU32_001733"
     CDS             complement(7022..8119)
                     /locus_tag="VQU32_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2473153.1"
                     /translation="MLERLKSIHYMFWASLIFMIFPILPVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLFWGIMLIYVAGNTAFVAVNYIWFFFFLSNLLSYHFSVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYLLGILTFVDLMTLGSVRIRLVEDLKEAQ
                     TKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQIQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVEIANQLDTASLTQEL
                     ESTASMILLELVTNIIKHAKASKAYLKLERTDQELLLMVRDDGKGFETVTENELHTVR
                     ERVLPFSGEVSVISQKHPTEVQVRLPYKERK"
     gene            complement(8119..8856)
                     /locus_tag="VQU32_001734"
     CDS             complement(8119..8856)
                     /locus_tag="VQU32_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2473154.1"
                     /translation="MKRWIALNKIEFLLTKRQSVYYLLSVGMPTAFYLFFSGMYQDTP
                     GGPANFMRDYLISMTAFSMMSTAIFSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMMVDYLVSILVVFSVGHLVRGVDMSLGSWIGAAFLLIVGSIAFVALGLTLTLLPSS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAIGKCLPTYQLMELLKTFLNEGSINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(8858..9742)
                     /locus_tag="VQU32_001735"
     CDS             complement(8858..9742)
                     /locus_tag="VQU32_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2473155.1"
                     /translation="MNMIKVQGLHKNIKSKAILKDISFEVAEGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVYIQDLPVTSSKLDYTRSYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLNNQEIDQLLQFDQQQKEQFAEKLSGGQKRLLSFILTLIGRPKLVFLDEPTAAMD
                     TSTRQRFWEIVRDLKAQGVTILYSSHYIEEVEHTADRILVLNKGELIRDTTPLAMRSE
                     EIEKHFILPLAYKEVVEKSVLVENWVQKQDALQVVTCEADAFWELLVQAGCRIQEIEV
                     NNRSLLDTIFEETQKGDD"
     gene            complement(9729..9923)
                     /locus_tag="VQU32_001736"
     CDS             complement(9729..9923)
                     /locus_tag="VQU32_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473156.1"
                     /translation="MKKAILFLFIGLSLVVWLVEMFTGWFDQSFLHQVIRGAWGLGFM
                     ISVVFPMGMEWLKGEYYEHD"
     gene            complement(9920..10126)
                     /locus_tag="VQU32_001737"
     CDS             complement(9920..10126)
                     /locus_tag="VQU32_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473157.1"
                     /translation="MEEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEGFYHFPWELFAS
                     FGIMSWLIREGLKLVRDVKKELEK"
     gene            complement(10611..10684)
                     /locus_tag="VQU32_001738"
     tRNA            complement(10611..10684)
                     /locus_tag="VQU32_001738"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10648..10650),aa:Pro,seq:tgg)
     gene            complement(10700..10773)
                     /locus_tag="VQU32_001739"
     tRNA            complement(10700..10773)
                     /locus_tag="VQU32_001739"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10737..10739),aa:Arg,seq:acg)
     gene            complement(10783..10868)
                     /locus_tag="VQU32_001740"
     tRNA            complement(10783..10868)
                     /locus_tag="VQU32_001740"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10832..10834),aa:Leu,seq:taa)
     gene            complement(10876..10947)
                     /locus_tag="VQU32_001741"
     tRNA            complement(10876..10947)
                     /locus_tag="VQU32_001741"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10913..10915),aa:Gly,seq:gcc)
     gene            complement(10970..11042)
                     /locus_tag="VQU32_001742"
     tRNA            complement(10970..11042)
                     /locus_tag="VQU32_001742"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11007..11009),aa:Thr,seq:tgt)
     gene            complement(11061..11142)
                     /locus_tag="VQU32_001743"
     tRNA            complement(11061..11142)
                     /locus_tag="VQU32_001743"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11106..11108),aa:Leu,seq:tag)
     gene            complement(11148..11220)
                     /locus_tag="VQU32_001744"
     tRNA            complement(11148..11220)
                     /locus_tag="VQU32_001744"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11185..11187),aa:Lys,seq:ttt)
     gene            complement(11245..11317)
                     /locus_tag="VQU32_001745"
     tRNA            complement(11245..11317)
                     /locus_tag="VQU32_001745"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11282..11284),aa:Asp,seq:gtc)
     gene            complement(11320..11392)
                     /locus_tag="VQU32_001746"
     tRNA            complement(11320..11392)
                     /locus_tag="VQU32_001746"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11357..11359),aa:Val,seq:tac)
BASE COUNT         3418 a         2641 c         2106 g         3229 t
ORIGIN      
        1 caagccctat ctacgatact gtttcaagaa acgagtcatc ttttcttgga tttgggctaa
       61 ctcatcaaca cgaggaaggt aaacgatacg gaagtggtct ggttcctgcc agttaaagcc
      121 tcgaccatga accaagagaa ccttttcctg cttcaagaaa tcaaggacaa actgctcatc
      181 atcatcgata cggtacatat tgcggtcgat ttttgggaag atatagagcc ccgccttggg
      241 tttaacggca gacaaacctg gaatatcttg aatggcatta tagatgaaat ttctttgctc
      301 gtagattcgt ccaccaggaa gaagcaattc atcgactgat tggtgacccc ccaaggaagt
      361 ttgtacgact tgttgggcca aaacgttaga gcaaaggcgc atattggaca gcatattgag
      421 cccttcgata tagcccttaa catgagtctt agggccagac aagaccatcc aacccacacg
      481 gaaacctgct atgcggtggg attttgacag accattcatg ctgacacaga agacatctgg
      541 tgccaagctc gccacaggcg tatgcacatg tccgtccatt accatgcggt catagatttc
      601 atccgcaaag ataatcaaat cgttttgacg ggcaatctca ataatctcca gcaagagttc
      661 cttaggataa agggctccag ttgggttatt tggattgata aggacaattg ccttggtatt
      721 ggaagtgatt tttgacttga tatcgtcaat atctgggtac cattccacag cttcatcaca
      781 gatatagtga acggcatttc ctccagctag gctgacagcc gccgtccata gaggatagtc
      841 tggcattggc actaagacct catccccatt gtccaaaagc ccctgcatgg acatgacaat
      901 caactcactg acaccatttc caaggtaaat atcatcaata tctacgtttg ggaatttctt
      961 cagctggcaa tactgcatga tggccttacg ggctgagaaa atccctttgg agtcagaata
     1021 gccttcacta tcacgcgcat tcataatcaa gtcatgaatg acctcgtctg gcgctgtaaa
     1081 gccaaattct gctggatttc ctgtattcag acgtaaaatc ttttctccat tcgctcgcat
     1141 ccgcatggct tcttccaata caggaccacg gatatcataa gcaacatgct ctaacttact
     1201 agacttgtta tattctttca tcttgtttcc tcaattcatc aggatagtaa cattatacca
     1261 ctagaaagag aatgcgacaa gtttactgaa atgtgttact agtttcgtat agaagaaaaa
     1321 ccttgctaaa ggcgaggtta agaacgtaga gatagcaggc attttgcaaa gacacatcac
     1381 ataccaaaag ataaacctag aaaaagcaag cctagaaagg agtcataaag aagattaaac
     1441 atgaggaaaa gcccccatat catcaagtaa tcccaacgcc cacttcgttt tgcaactaga
     1501 aataagatta gatggtagag tacatgaaag actaaaaatc caataaaact tggataaaga
     1561 agcggtacca gactctctaa ttgacagatc atgatcactt ttaccaagac tgaaactagg
     1621 ataattccat aatacaggaa atttgatttt tgaaaagagt ttttcatcat tctcccttcc
     1681 ttcacttctt tccttctatc ctttctttta ttttatcata tatttatata gattttaagc
     1741 cctaatattc ttttgaaaaa tacttttttt gctttacttc tatagtgaaa aggtttacaa
     1801 ttatagtaag aaaatttcag gaggtttcat tatggcacaa cgttaccaaa atatcatggt
     1861 cgcaatcgat ggttctaagg aagcggactt ggcttttgtc aagggagttc attctgctct
     1921 acgaaacgac gctaaactca ccatcgcaca tgtcattgac acacgcgctc tccaaagcgt
     1981 atccaccttt gatgctgaag tttacgaaga actccaagtc gacgctgaaa gtctgatgaa
     2041 agagtacgaa aaacgtgcta aagatgctgg ggtagcagat gttcatatcg tcattgaaat
     2101 gggaaatcca aagaccctgc tagcacgtac tattccagat gccgaggaag tggacctcat
     2161 cctcgttggc gcaactggtc tcaacgcctt tgaacgcctc ttggtcggct cttcatctga
     2221 atacatactc cgccatgcta aggtcgattt gctggttgtg agagaacaag aaaaaacctt
     2281 ataatcacaa agaaaaggag cccctagctc ctttttgttt acgatttatt tctctcttta
     2341 tggcgttcgt aagccttgag ctggcgctgc agttcctttt taatagcagg ttctggagca
     2401 tatttttctt cccaattatc tggttttaag attttatggg tcactggatc aaaatgagcc
     2461 ttgccatctg gaaaaatttt ccccatattg gcctgatgga caatatcaaa aatacgttct
     2521 gggtccaccc ccatcaagac aaaactgccg taggtgaagt aaagcgtgtc aatcaaggca
     2581 tccacttgcc ctatcaaatc ttgctgagca ggtgtcttct tggctacttt acctgctgcc
     2641 ttatcaaggg cctgatgaag ttgcgataca gcttgaccaa aatcttcttc agaaggactg
     2701 gctgctcgaa caaactccac caattcttct attttaaaac cagccctatg ggttgcaccc
     2761 tctaaatccc aagctcgagg ttcttcttgg gttcgttcat ccatcatgtg gtggaaggtc
     2821 ttgaccttat tgaaatgata gtcacggctg acaaagactt tttctgaaga caaaacacca
     2881 aaatgttcca aggccttgtg gatcccatct tgttgattgc tagtggtaat gtgcttggca
     2941 gcttccttga cactgctact accatttccc atagcgaccg acataccgac gccagccagc
     3001 atttcaaggt cattatctga gtcaccaaaa gccatgactt ggttgaggtc aaagccatat
     3061 tctttcccaa ctcggcgaat gccttctaat ttagaatttc cctgattgat gacatccgat
     3121 gcaaaaggat tgctacgtgt caatttcaag tcttcaaaat cagctgccgc cttctcagat
     3181 tcttctggtg tcatcagcat caaaacttgg tagataggct gattcatcag gtgaagcagg
     3241 tcctcttcct tttggggaac aaccttgctg accatgcgat taaaagactg actcaccgtc
     3301 cgagttaaaa cagagggaac gaagcgacta attcgttggg aaaaagaacc cagaccaaag
     3361 gacatgattt tagaacccaa catggcatcc ttggtcccta gggcaatctc cgtgccctct
     3421 tttttagcat agctaattag atggcgcaaa tgtaacttgg aaatagggct cgtgaacaag
     3481 actctgtctt tactaaagat atactggcca ttataggtta ccgcaaaatc cagatccaaa
     3541 tcgtccatca attccttaac aaaaaaaggt cctcgccctg tcgctacgcc aactagtacc
     3601 ccttgttctt tgacaatctt aatcgcgtcc ttagtggatt tcaaaacact cttgcgattg
     3661 ttgaccaagg ttccatcgat atcaaaaaaa acagctttga cttccatcct attccaatct
     3721 ccccttttgt gatacaatga ttataccaca tttcagaaag agtgagtaaa tcatgcctaa
     3781 gaaaatcctt gttttacata cgggtggaac tatttccatg caggccgatg cttctggcgc
     3841 tgttgtgacg agttcagata atcccatgaa ccatgtgtcc aacccacttg aaggaatcca
     3901 agtccacgcc ttggactttt ttaaccttcc aagtccccat atcaaaccca aacatatgct
     3961 ggtcctctac cagaaaatta aagaggaagc agataactac gatggagtgg taatcacaca
     4021 cggaaccgat actttagagg aaacagccta tttccttgat accatggaag ttccccatat
     4081 gcctatcgtt ctaacaggag ccatgcgtag ctccaatgag ctcggtagtg atggtgttta
     4141 taattaccta agtgctttac gagtggccag cgatgacagg gctgctgaca aaggagtttt
     4201 ggtcgttatg aacgatgaaa tccacgctgc caagtatgtc accaaaacac atacgactaa
     4261 tgtcagcacc ttccagactc caacacatgg ccccctcggt ctcatcatga aacaggaaat
     4321 cctctacttc aaaacagctg aacctcgtgt tcgctttgac cttgatcaca tacaaggttt
     4381 agtccctatc atctcggctt atgctggtat gacagatgag ctgattgata tgctggattt
     4441 agaacacttg gacggtttga ttatccaagc cttcggagct ggtaatattc ccaaagaaac
     4501 ggctcaaaaa ttagaaagcc ttctgcaaaa aggaatccca gtcgctctgg tatcacgatg
     4561 ctttaacggt attgccgagc ctgtttatgc ctaccagggt gggggcgtac agttgcaaaa
     4621 agcaggcgtt ttctttgtta aagaactcaa cgcccaaaaa gctcgcttga aactcctcat
     4681 cgccctcaat gccggactaa caggacaggc tttgaaagac tatatggaag gctaatactc
     4741 ttcgaaaatc tctgcaaacc acgtcagagt cgccctaccg tatgtatggt tactgacttc
     4801 gtcagttcta tctacaacct caaaaacatg ttttgagctg acttcgtcag tttcatctac
     4861 aacctcaaaa cagtgtgttg cgcagccttt aaccagccgc ctagtccgct ctatggtatt
     4921 cattaagtca acatctcttg tttaagagca caaaatcagg aaatcttctc gattccctga
     4981 ttttttctat ttacgttttc gtgttgagct acgttctgtc aaaccatgag gtaagagaac
     5041 ttcacgttct tccaactctt ccttatgcat aatcttggtc aacatacgca tactaatggc
     5101 accaaggtca taaagaggtt gggcaatcgt tgtcaagttt ggacgggtaa agcgtgagat
     5161 ttgtgaatca tcactagtaa taatttcaaa atcttctggc acagaaacac ccttatcagc
     5221 caaaccgttc aagactcctg ctgccaactc atcacctgtc acaactgctg cagttgcatt
     5281 tgatgaaatc aaacgctctg ctaaggcgta accatcatca tagctatatt tagattcaaa
     5341 taccaaaccc tcactataag tgattcctac ttttttcaag gtttccttgt agccaactaa
     5401 acgaacctta ccattgatgt catccactag cggaccgcta acgaaagcaa tacgctcatt
     5461 ttctttagca aggtagctca ctgcatcaat tgttgcttgc ttatagtcaa tattgacact
     5521 tggcaactgg tgctcaacat cgacagttcc tgcgagaaca atcggagtac gtgaacgcga
     5581 aaattctgag cgaattttat ctgtcaagtg ataacccata tagataatgc catctacctg
     5641 ctttgaaaag agggtattga caacagaaac ttctttctcg ttatcttcat cgctattagc
     5701 taggacaata ttgtacttgt acatttctgc aatatcatca atccccttag ccaaactcga
     5761 aaaataacca ttggtaatat ttggaatcac gacaccgaca gtggttgtct ttttacttgc
     5821 aagaccacgc gcaactgcat ttggacgata atccaaacga tcaattacct ctagcacttt
     5881 tttacgggta ttctctttta catttttatt gccattgacc acacggctga ccgtcgccat
     5941 ggaaacacct gcttcacgag cgacatcata aatggttact gtatcatctg cattcattcc
     6001 ttttcctgtc ctttctatct cacacattct tttacaagta gaggtactga ttgaagctct
     6061 atatctactt acaaaagtga agatgtgaaa atttcgtttt catatttcta cttattccat
     6121 tctatcacta attgtaaaca ctttcaagtg ttttttgaag attgattgaa aaaatttcat
     6181 agaaaaccta ggtttagctc cttgctacca ccttagacta aataaaaagg aggaaactaa
     6241 gcccttctaa agttatagta aaatgaaata agaacaggat aaatcgatca ggacagtcaa
     6301 atcgatttct aacaatgttt tagaagtaga ggtgtactat tctagtttca atctactata
     6361 ggtattgttc cattcactac cgtcaatttt agcacatagt cttcatgaaa atattatatc
     6421 atcataacca accagattct tttgcgatat tagctgcctc tgttcgatta ccagcatcca
     6481 gttttgaaag aatattggtg acatagtttc gaatagttcc gtttgagagg taaagctgat
     6541 ctgcgatttc ttggttggat aagccccggg cgattccctt taagacagca atctcttgtt
     6601 ctgtcaacgg attgggacgg gtcatcacca cttccatcaa ttcaggcgaa tactccttgc
     6661 gtccttcgag gacggtgtgc aaggtttgca tgaggtctgc aatgcttcgt tcctttaata
     6721 cataagcatc cactccagcc ttgaccgcac gttcaaaata cccagcacgc ttgaaggtcg
     6781 tcaccacaac cacctttgtt tcaagccttt ctgatcgtat ccactccaag acttcaagac
     6841 ctgtcttaac aggcatttct acgtcaagga tggcgatatc tacagactcc ttttctaata
     6901 gttggattgc ttcttgccca ttcttggctt gaaggacaga ctccacatct ggttgaagcg
     6961 tgagcaattg acacatagca tctcgcaaca tactttgatc ttctgcgact aatactttca
     7021 tctactttct ctccttataa ggtagtcgaa cttgcacttc cgttggatgt ttctgactga
     7081 ttacacttac ttctcctgaa aatggaagga cacgttctcg aactgtatgg agctcatttt
     7141 ctgttacagt ttcaaaacct ttcccgtcat ctctgaccat caggagcaat tcctgatctg
     7201 tgcgttctaa ttttaagtag gctttagacg ctttagcatg tttgatgatg ttggtcacta
     7261 attcaagcaa aatcatggaa gccgttgact ctaattcctg agttaagcta gctgtatcta
     7321 gttgattagc tatttccacc tcaattccag caatttctaa catctttttc acagtctcta
     7381 gttcggatgt caaagttcta gacttaagat tttccacaat ggttcgcact tcacgcattg
     7441 attctttgct aatctgctgt atttctctta attccttttc cacttgtgga taagcctgta
     7501 tttgaaataa ctgcaaggct agatcagttt taacactaag catagcaaag gtatgcccca
     7561 gactatcatg caaatcttga ccgatacgat tgcgctcatt ttctgctagc aatagattta
     7621 tctgggtatt ttgcttagtc tgagcttctt tcaaatcctc gacaaggcga attcgaaccg
     7681 agcccaaagt catcaaatca acaaaagtaa gaattccaag cagatagact agaaactcaa
     7741 cttcgattct ctgaaaaatc aaaagtcgcc ccacaacaag gacttgagca agaagaaaag
     7801 tccagacatg taaagacttt aaactacgta cgctgaaatg ataacttaag agattggaaa
     7861 ggaaaaagaa aaaccagata taattcacag caacaaaggc ggtattccca gctacataga
     7921 tcagcatgat cccccagaaa agccaagata ggcgctggct cttagttgtt aaaacaccca
     7981 aatatgccac tacaaataga atatcaatca ataaatgcca ggcagaaagc cacccagtca
     8041 ctacaggtag gatggggaaa atcataaaaa ttaaactggc ccaaaacata tagtgtatgc
     8101 ttttcagtct ttcaagcatt aagcattctc cttatgacct tgaaggtaaa tggtcaaacc
     8161 aaacaaaact gctgaaaaaa caagtagata aactgtggct gatagattga tgctaccctc
     8221 gtttaagaag gtcttgagca actccatcaa ctgataagtt ggtaggcact tcccgattgc
     8281 ttgcatccag tctggaaata aagagatggg catccagagt ccacctaaaa cagccaagcc
     8341 tagatagaga agattgccca cgacagacat cagctgacta gaaggcaaga gtgtcaaggt
     8401 caagccaagc gctacaaaag ctatacttcc tactatcagc aagaacgcag ccccaatcca
     8461 gcttcctaaa gacatatcca cacctctgac caagtgccca actgagaaaa caaccaggat
     8521 tgagaccaaa taatcaacca tcatacttgt tatctttgat agataatatt ctaccatatt
     8581 tacagggcta tgacgcaatg ttttctgcca gttgttcatc ttatcggtat gtaagacagc
     8641 tgggaatgaa aagatagcgg tcgacatcat ggaaaaggca gtcatggaga tgaggtagtc
     8701 gcgcataaaa ttagccggtc cacctggtgt gtcctggtac atgcctgaaa agaatagata
     8761 gaaggctgtc ggcatcccta cagataataa ataatagact gattgccgtt tggtcaatag
     8821 aaattctatc ttgtttagtg caatccatcg tttcatctta gtcatctccc ttctgtgttt
     8881 cttcaaagat tgtatccaac aaactacgat tattgacttc gatttcttgt atcctacatc
     8941 ctgcttgaac taacagttcc cagaaagcat ccgcttcgca tgtgactact tgtaaggcat
     9001 cttgtttttg tacccagttt tcaaccaaga ctgacttctc aacgacttcc ttgtaagcta
     9061 gcggaaggat aaaatgcttt tcaatctcct cactacgcat agctagaggc gtcgtgtcac
     9121 gaatcaactc tcccttattt aaaaccaaaa tccggtcagc tgtatgctct acctcttcaa
     9181 tataatgaga cgaatagaga atggtgactc cttgcgcttt taggtcccga acaatttccc
     9241 aaaaacgttg acgagttgaa gtatccatgg cagcagttgg ctcatctaaa aagacaagct
     9301 ttggtcgccc aattaaggtc aagataaaag ataaaagacg cttttgccca cctgacaatt
     9361 tttctgcgaa ttgctctttc tgttgctggt caaactgtaa tagttgatcg atttcctggt
     9421 tgttcaaggg atttggatag atacgttgaa agaaagcaat caactcttta acctttagtt
     9481 tttgaacaat tacattttct tgagggagat aggatctcgt atagtctaac ttagagctcg
     9541 ttaccggcaa atcttggata tatacttgcc cactggtgac caatttatct ccaagcaaac
     9601 agtccaagag tgtcgtctta ccagctccat ttggcccaat caaggcgacg cattcacctt
     9661 cagctacctc aaaggaaata tccttcaaaa tagccttact tttgatgttt ttatgtaagc
     9721 cttgtacctt aatcatgttc ataatattct cctttcaacc actccattcc catagggaaa
     9781 acgacggaaa tcataaatcc taatccccag gcgccgcgaa taacttggtg aagaaaggat
     9841 tgatcaaacc aacctgtaaa catttctacc aaccatacca ctagtgatag tccaataaaa
     9901 agaaagagaa tcgctttttt catttttcaa gctccttttt cacatctctt actaatttca
     9961 agccttctcg tatcaaccaa gacatgattc caaagcttgc aaacaactcc caaggaaaat
    10021 gatagaatcc ttcgtccaat cctgagaaca tgagataagt catgactcct gctactacta
    10081 aactcactgc gataatcatt ttatttctca tctcttcttc ctccatttca tactacaatt
    10141 atagtctttt gaaatcagag gagatagaag cttctgtcac taaaaaatat gacaaatgtc
    10201 ataaaaaatt ctgttcaaaa caagcaagat acactataca ataaaacaca attagataaa
    10261 tctaaggcaa cttcctcaaa agagatatca aaccccattc acactataat gtaaactaat
    10321 acttatttaa agtcaaaaga gcggaaattt ttatcgtaca aacatatata ttataggtaa
    10381 gacaaaaaac tcttcaatta ctttcttaaa aatttatagg agttataaaa tctattaaat
    10441 ttcaatgtgg atacctaaac tacagtattt attgaaaaga ctggagacaa aaagtatacg
    10501 ctgccaaaat gaattgctga aaatcaaaaa agagagaagc aaactgattc tctcttaatg
    10561 tatataatat ctagttataa aaatacacac tcacatatct ctgtaatgaa tcgggaagac
    10621 aggattcgaa cctgcgacac cttggtccca aaccaagtac tctaccaagc tgagctactt
    10681 cccgagttaa atagaaaaat gcaccctaga ggagtcgaac ctctaaccgc ctgattcgta
    10741 gtcaggtact ctatccagtt gagctaaggg tgctccatat tatgccgagg accggaatcg
    10801 aaccggtacg atcgttacca atcgcaggat tttaagtcct gtgcgtctgc cagttccgcc
    10861 accccggcct ctctaagcga acgacgggat tcgaacccgc gacccccacc ttggcaaggt
    10921 ggtgttctac cactgaacta cgttcgcact gttttcttct atctaaaaat gccggctaca
    10981 tgacttgaac acgcgaccct ctgattacaa atcagatgct ctaccaactg agctaagccg
    11041 gctcatttgt tatatcttaa tgcgggttaa gggacttgaa cccccacgcc gttaagcgcc
    11101 agatcctaaa tctggtgcgt ctgccaattc cgccaaaccc gcatatatga cccgtactgg
    11161 gctcgaacca gtgacccatt gattaaaagt caattgctct accaactgag ctaacgagtc
    11221 taaaataact tgcgttacct taaacggtcc cgacgggaat cgaacccgcg atcttcgccg
    11281 tgacagggcg acgtgataac cgctacacta cgggaccttt gggagttaac gggatcgaac
    11341 cgctgaccct ctgcttgtaa ggcagatgct ctcccagctg agctaaactc ccta
//
LOCUS       DAVBBS010000059        11268 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVBBS010000059 DAVBBS010000000
VERSION     DAVBBS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11268
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.059"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..77
                     /locus_tag="VQU32_001747"
     CDS             <1..77
                     /locus_tag="VQU32_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2473158.1"
                     /translation="MPQSPWVERIVQVLDLDYEDLFRR"
     gene            80..667
                     /locus_tag="VQU32_001748"
     CDS             80..667
                     /locus_tag="VQU32_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2473159.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCSDELTILQILYGASLIS
                     FLWMYGQRKQVIKVNMKTRIQWLGIGFVSLLIISLCFSLIHAQESTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYV
                     SLGMFLGLVRYKLDLWGSMGLHLVWNLLVYSLLLF"
     gene            1066..1875
                     /locus_tag="VQU32_001749"
     CDS             1066..1875
                     /locus_tag="VQU32_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2473160.1"
                     /translation="MLLQKELIPMIEANLPNMAYAEKDIAKFFLKQQPLNNYSCKALC
                     ECLNVSKATLTRFAKKCGFKGFRQFIFKYQEMIREKEKLALYTEATEKVLSDYEEMLR
                     KTYTVLDEVQLERIAEMIETAERVYLYGKVSSVLALQEMKMRFMRLGVIGEVLSDEDM
                     ILWNSLLLNENCLVIGASVSGQTDIVLEGLQKAADKGAKTVLMTTRKFDEEDCFFDEL
                     LLLASTNHLSYGNRISPQFPILLITDCLFSHYLESPERQYYYNQTIIHKEE"
     gene            1880..2581
                     /locus_tag="VQU32_001750"
     CDS             1880..2581
                     /locus_tag="VQU32_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2473161.1"
                     /translation="MSQIWTKEKFISQVHGGVIVSCQALPGEPLYNEEFSLMPFMAKA
                     ALEAGAVGIRANSVRDIKAIQKVVDLPIIGIIKRDYPPQEPYITATMKEVDELVECGT
                     TVIAFDATLRPRYDGLVINEFIKKIKEKYPNQLLMADVSNFDEGLYAFKSGVDFVGTT
                     LSGYTSTSVQSDEPDFELMKKLADFNIPVIAEGKIHYPEQLKKAYSLGVTSVVIGGAI
                     TRPKEIAQRFINVIK"
     gene            2606..3523
                     /locus_tag="VQU32_001751"
     CDS             2606..3523
                     /locus_tag="VQU32_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HET2473162.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQERVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHVACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAAPNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            3551..5092
                     /locus_tag="VQU32_001752"
     CDS             3551..5092
                     /locus_tag="VQU32_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HET2473163.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGNSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGAVAIVYSYTGGLKSVLWTDFIQGVILISGVVLALFVLMANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLATATVFYLIGTGLYVFYQVQNAGSAASNIPQDQIFMYFIAYQLPVGITGL
                     ILAAIYAASQSTISTGLNSVATSWTLDIQDVISKNMSDDRRTKIAQFVSLAVGLFSIG
                     VSIVMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTGNKVSAPKYTTIHDIPEIKADSS
                     WEVRH"
     gene            5111..5569
                     /locus_tag="VQU32_001753"
     CDS             5111..5569
                     /locus_tag="VQU32_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2473164.1"
                     /translation="MIISEQSDFRRYASINKHFSKVCDFLENTNLTDLVDGKVDIDGE
                     NVFANCMIYLADGVPGDIFETHKKYLDIHIVVENTEKMAVTSPVRAQSRVPFSEEKDI
                     AFYDSKDYQIVELLPGNMLVTFEEDLHQPKIHCNDEPVKKLVIKVLNEEK"
     gene            5571..7793
                     /locus_tag="VQU32_001754"
     CDS             5571..7793
                     /locus_tag="VQU32_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HET2473165.1"
                     /translation="MKKNIKQYVTLGTAVVLSAFVANSVAAQETETSEVSTPELVQPV
                     APTTPISEVQPKSGNSSEVTVQPRTVETTVKDPSSATEETPVLEKNNVTLTGGGENVT
                     NELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNHLVSRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPVPLEWPRDVGGRDLQISGGATYIDSVIVEKNNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLKKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFKNNKKTEYRNGTHVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSTDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN
                     "
     gene            7833..8942
                     /locus_tag="VQU32_001755"
     CDS             7833..8942
                     /locus_tag="VQU32_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2473166.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEYEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            9099..9989
                     /locus_tag="VQU32_001756"
     CDS             9099..9989
                     /locus_tag="VQU32_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2473167.1"
                     /translation="MKIIGIDIGGTTIKADLYNEFGTSLNQFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYILNHSIDGVGISTAGVVNANTGEIIYTGYTIPGYIGVNFTFEIEKRF
                     GLSTFVENDVNCAALGELWKGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLRAGDKVAKETFEIFVE
                     NLTKGLLTITYLLNPEILILGGGILAKKDILLPEIQSSLAKNAMDNRFLPKNLVAATL
                     GNEAGRIGAVKNFLDRISNK"
     gene            10014..10337
                     /locus_tag="VQU32_001757"
     CDS             10014..10337
                     /locus_tag="VQU32_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473168.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELVSIESNLEMKQQNLIDEVNKTIKENYEKIQYISSSRRGEFVEKIIGRVV
                     EKYGY"
     gene            10327..>11268
                     /locus_tag="VQU32_001758"
     CDS             10327..>11268
                     /locus_tag="VQU32_001758"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit I is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HET2473169.1"
                     /translation="MAISQMKRISLLFSKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFPTIQISHTSNSNHGVIEGNDALIYLMNQQQYLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNRQINRVHELERHIQSNNEEIERLIKW
                     EKLEIIPAHLEQFFFCKGKVGTIPRTVDNRLYNSFLENNIEVQEIFSNDREYGVVVFY
                     QSSCSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKNNLID
                     LQWQIDYILSIYARQISKNNLLSTPHLVALEGWIEETR"
BASE COUNT         3873 a         1616 c         2202 g         3577 t
ORIGIN      
        1 gaatgcctca aagtccatgg gtagaaagga ttgtgcaggt attagattta gattatgaag
       61 atttatttcg gaggtaatta tgaaaagaat aattccagtt tatatattcc aacaagtaaa
      121 tgtcctattg gtatctctat acttattgaa atttctttgt agcgatgagt taactatact
      181 acagattctc tatggagcgt cactcatttc ttttttatgg atgtatggtc aacgaaaaca
      241 agtgatcaag gttaatatga aaactaggat acaatggctt ggtattggat tcgttagcct
      301 actgattata agtctatgtt ttagtctaat ccatgcgcaa gaaagcacga atcaagcaaa
      361 cttaattgga cttcaacatc aaattccttg gttttcattt ttattgtttt taatcaatgc
      421 gagtatggtt gaagaatttc tgtatagaga aattttatgg aacttggtta gaaaattaga
      481 tattcgagtt gctttgacaa gtgttttatt tgccttagca catcatccag gaaccattat
      541 agcttggtgt ttgtatgtat cgctggggat gtttttaggg cttgtacgct acaaattgga
      601 cttatggggc agtatggggc tacatttggt gtggaaccta ctagtttata gtttgctgct
      661 tttttaaaat aagtgatctg atttatctca taaatcagta gtttttatag gattattttt
      721 aattcggaaa caaaaatgta ttaacagcat aaaataaaga aactaggaac atttttttta
      781 ttttttagaa ataaaatgtt gtatagaaat tccgaaagaa ataataaaat caaaataaaa
      841 attaataaaa taggtatatt tgaacatatt gacgtcgaca aatgttcttg caagacataa
      901 aatacaaata aaaagaataa aaaattttta tatttttata tttgttgttc tgttataaaa
      961 tgaaagaaat tagaaactag tttctaaatc atgaaaaata ttgaaaaaaa aaaaataagt
     1021 gttaaaataa agaatgtaaa cgtttacttc aataaaggag ctcatatgtt actgcaaaaa
     1081 gaactaattc caatgataga agctaactta ccaaatatgg catatgctga aaaagacatt
     1141 gctaaattct tcttaaaaca gcaacctctg aataattatt catgtaaggc attgtgcgaa
     1201 tgccttaatg tatccaaagc aacattgact cgatttgcga aaaaatgtgg ttttaaaggt
     1261 tttagacaat tcattttcaa ataccaagag atgattcgtg agaaagaaaa gttagcattg
     1321 tatacagagg ctacagaaaa agttttatcc gactatgaag aaatgttgag aaaaacttac
     1381 acggttcttg atgaagttca acttgagcgt attgctgaga tgatagaaac tgctgagcgt
     1441 gtatatcttt atggtaaagt aagttctgtt cttgctttac aagaaatgaa gatgagattt
     1501 atgcgtctcg gagtcattgg tgaagtatta tcagacgagg atatgatttt gtggaatagc
     1561 ttattactta atgaaaattg ccttgtcatt ggagcatccg tttcaggtca aactgatatt
     1621 gtactagaag gtcttcaaaa agctgcggat aaaggcgcta aaacagtttt aatgactaca
     1681 agaaaatttg acgaagaaga ttgtttcttt gatgaactat tgttattagc ttcgaccaat
     1741 catctctcgt atggcaatcg catatcacct cagtttccaa tacttttaat tacagactgc
     1801 ttattctctc attatctgga aagtccagag agacaatatt attacaatca aactattatc
     1861 cataaggagg aataataaaa tgagtcaaat ttggactaaa gaaaaattta taagtcaagt
     1921 ccatggtgga gtcattgttt cttgtcaagc tttacccggt gaaccccttt ataatgaaga
     1981 atttagcttg atgcctttta tggctaaagc agctttagag gcaggagcag tgggcattcg
     2041 cgcaaattct gtgcgtgata ttaaagcaat tcagaaagta gtagatttac caataattgg
     2101 aattatcaaa agagattatc cacctcaaga accatatatt actgctacga tgaaagaagt
     2161 agatgaactt gtagaatgcg gaacaacagt cattgcattt gatgcaactt taagaccaag
     2221 atatgatggc ttagttatca atgaatttat caaaaaaata aaagaaaaat atcctaatca
     2281 attgttgatg gcggatgtaa gtaattttga tgaaggtctc tatgcattta aatcaggcgt
     2341 tgattttgtt ggtacaacat tatcagggta cacaagtaca agtgtacaat cagatgagcc
     2401 tgattttgaa ctaatgaaaa aattggctga ttttaatatt ccggtaattg ccgaaggaaa
     2461 aattcattat ccagaacaat taaaaaaagc ttatagttta ggtgttacca gtgttgtcat
     2521 tggtggagcg attacacgtc caaaagaaat tgctcagcga tttattaatg tcatcaaata
     2581 aaaaacaata taaaggagaa atctgatgaa agatttaact aaatacaaag gcgttattcc
     2641 tgcattttat gcttgctatg atgaaaatgg tgaaattagc caagaacgtg taaaatctct
     2701 ggtgcaatat ttcattgaca aaggtgtaaa aggtatctat gtaaatggtt cttcaggtga
     2761 atgtatttac caaagtgtag aagatcgtaa acaaattatt gaagctgtta tggaagttgc
     2821 taaaggtaaa ttaacagtta tcaaccatgt tgcatgtaat aacacgaaag atagtattga
     2881 attggcaaaa cattcagaaa gtgttggagt cgatgctatt gctgctatcc cacctattta
     2941 cttcaaattg ccagaatatt caattgcagc atattggaat gcaatgagtg aagctgcgcc
     3001 aaatacagat tttatcattt ataatattcc acaattggca ggggttgcgt tgactggtag
     3061 tttgtatgca acaatgcgtc aaaatcctcg tgtgattgga gttaaaaatt cttctatgcc
     3121 tgtacaagat attcaaatgt ttgtagctgc aggtggagaa gattacattg tattcaatgg
     3181 tccagatgaa caatatcttg gtggtcgctt gatgggagca gaagctggta ttggtggtac
     3241 ttatggcgtt atgccagatt tgttcttgaa attggaaagt ttgattcaag aacgagattt
     3301 agatactgct aaaaaacttc aatatgctat caatgaagtt atctataaga tgatatcagg
     3361 taaggcaaat atgtatgctg tagcaaaaga ggttttgcgt ctaaatgaaa aacttgattt
     3421 aggttctgtt cgtcaaccct tagaagcatt ggcagaaggt gacttggaag ttgcaaaaca
     3481 agcagcagaa cttattcaac aagcacgaaa agaattttta taataaataa aatcaatatg
     3541 gagatatgtt atgggcacaa caggatttac aataattgac ttaattatct tgattgttta
     3601 tttacttgcg gtgttggttg caggtatcta tttctctaaa aaagagatga aaggaaaaga
     3661 gttcttcaaa ggagatggtt cggttccttg gtatgttact tcggtatcca tttttgccac
     3721 aatgctcagt ccgatttcct tcttgggact cgctggtaac tcttatgcag gtagctggat
     3781 tttatggttt gctcaattag ggatggtagt agctattcca ctgacaattc gttttatctt
     3841 acctatcttt gcacggatag acatcgatac ggcatatgat tacttggata aacgttttaa
     3901 ttctaaagca cttcgtatta tttcagcact cttgtttatt atttatcaat tgggacgtat
     3961 gtctatcatt atgtacctcc catcagctgg tttatcagta ttgacaggaa ttgacatcaa
     4021 tattttgatt attttgatgg gtgcagttgc aattgtttat tcttacactg gtggtctaaa
     4081 atccgtatta tggacagact ttattcaagg tgtgattctg attagtggtg tcgttttagc
     4141 tttatttgta ctgatggcta atattaaagg tggctttggt gcagtagcag aaacattagc
     4201 aaacgggaaa ttccttgctg caaatgaaaa acttttcgat cctaacttgc tttcaaactc
     4261 catcttttta attgtgatgg gttcaggctt tacaatcttg tcttcctatg cttcatctca
     4321 agatttggtt caacgtttta ctacaacaca aaatattaag aaacttaata agatgttgtt
     4381 cacaaacggt gtcttgtcac ttgcaactgc aacagtcttt tacttgattg gtacaggctt
     4441 gtacgtattc tatcaagtac aaaatgcagg tagtgcagct agcaatatcc ctcaagacca
     4501 aatctttatg tactttattg cataccagtt accagtaggt atcacaggtt tgattttggc
     4561 agcgatttat gcagcatctc aatcaactat ttcaacaggt ttgaactctg ttgcaacttc
     4621 atggacattg gatattcaag atgtcatttc taaaaatatg tcagacgatc gtcgtacgaa
     4681 aattgcacaa ttcgtatctc tagcagtagg tttattctca attggtgttt ccattgtcat
     4741 ggctcactca gatattaaat ctgcatacga atggttcaat agcttcatgg gacttgtact
     4801 tggtctactt ggtggtgtat ttattcttgg atttgtttct aaaaaagcaa ataaacaagg
     4861 tgcttatgca gcgctgattg tatcaactat cgtcatggta tttattaaat acttccttcc
     4921 tccaacagct gtgagctact gggcatattc attgatttca atctctgtat cagtagtttc
     4981 aggttatatt gtatctgttc ttactggaaa taaagtatct gcacctaaat atacaacgat
     5041 tcatgatatt ccagaaatta aagcggattc aagttgggaa gttcgtcact aatagaaaag
     5101 agaaaataat atgattattt ctgaacaatc tgattttaga cgatatgctt ctatcaataa
     5161 acatttttca aaagtctgtg attttttaga aaatacaaat ttgacagatt tagttgatgg
     5221 aaaagttgat attgacgggg aaaatgtttt tgcaaattgt atgatctatc tagctgatgg
     5281 agtcccagga gatatttttg aaactcataa gaaatattta gatattcata ttgttgttga
     5341 aaatactgaa aaaatggcag taacttcccc agttcgtgcc cagtcacgtg taccttttag
     5401 cgaagaaaag gatattgctt tttacgatag caaagactat caaattgttg aattgcttcc
     5461 agggaatatg ttggtgactt ttgaagaaga tcttcaccaa ccaaagattc attgcaatga
     5521 tgagcctgtt aaaaaacttg ttattaaagt tttaaacgag gaaaaataaa atgaaaaaaa
     5581 atattaaaca atacgtaacc ttaggtactg cagtggtatt atcagcattt gttgctaact
     5641 cagttgcagc tcaggagact gaaacttctg aagtatcaac accagagttg gtgcaacctg
     5701 ttgcaccaac gactccgatt tcggaagtac aacctaaatc gggtaactct tcggaagtta
     5761 ctgtacaacc tagaacagtc gaaactactg ttaaggatcc atcttctgca acggaagaaa
     5821 ctcctgtctt agaaaaaaat aatgttactt taacaggggg cggagaaaat gttactaatg
     5881 agttaaagga taaatttact agcggtgact ttactgtagt gattaagtac aatcagtcta
     5941 gtgagaaagg cttacaagct ctgtttggaa tatctaattc taaacccggt caacaaaata
     6001 gttatgtaga tgtgttcctt agagacaatg gtgaattggg aatggaagcg cgtgatactt
     6061 cttccaataa aaatcaccta gtatccagac ctgcttcagt ttggggtaag tacaaacaag
     6121 aagctgtgac taacactgtt gcagtagtag cagattcagt caaaaaaaca tattctttat
     6181 atgcaaatgg tacaaaagta gtagaaaaga aagtggataa cttcttaaac atcaaggata
     6241 ttaaaggtat tgattactat atgcttgggg gagtgaaacg tgcaggaaaa acggcgtttg
     6301 gttttaacgg aacactagaa aatatcaaat tctttaatag tgcattggat gaagaaactg
     6361 ttaaaaagat gacaacaaac gctgttactg gacatttaat ttatacggct aatgatacaa
     6421 caggttctaa ctatttccgt attccagttc tgtatacttt tagcaatggt cgggtatttt
     6481 caagcattga cgctcgttac ggcggaactc atgatttttt gaataaaatt aatattgcta
     6541 caagttatag tgatgataat ggtaagacat ggactaaacc aaaattaaca ttggcattcg
     6601 atgattttgc gccagtacca ttagaatggc ctcgtgatgt tggtggacgt gacttacaaa
     6661 tcagcggtgg tgcaacctat attgactctg ttattgttga aaaaaataac aaacaagtac
     6721 tcatgtttgc ggatgtgatg cctgctggag taagttttag agaagctact agaaaagatt
     6781 caggttataa acaaattgat ggtaattatt accttaaatt aaagaaacaa ggtgatactg
     6841 attacaatta tactattcgt gagaatggta ctgtatacga cgatcgtacc aacagaccaa
     6901 ctgaattttc agtagataaa aatttcggta ttaaacaaaa tggtaattat ttgacagtag
     6961 agcaatattc ggtttcattt aaaaataata aaaagactga atatcgtaat gggactcacg
     7021 tccatatgaa tatcttctac aaagatgcat tgttcaaagt agtgccaacg aactatattg
     7081 catatatttc tagcaatgat catggcgaat cttggtctgc accaacttta ttacctccta
     7141 taatgggact taatcggaat gcgccttatt tgggtcctgg acgtggaatc attgaaagct
     7201 caactggacg tattcttatt ccgtcttaca ctggtaaaga gtctgcattc atttatagtg
     7261 acgataatgg agcatcttgg aaagttaaag ttgtgccact tccttctagt tggtcagcag
     7321 aagcacaatt tgtagaattg agtccaggag taattcaagc atacatgcgt acaaataatg
     7381 gtaaaattgc atatttaaca agtacagacg caggtactac ttggagtgca ccggaatatt
     7441 tgaaatttgt ttcaaatcca agttatggaa cacaattatc aatcatcaat tatagtcaac
     7501 tgattgatgg taaaaaggct gtcattttaa gtactccaaa ctccacaaat ggtcgtaaac
     7561 acggacaaat ttggattggt ctaattaatg atgataatac aattgattgg cgttatcatc
     7621 acgacgttga ttatagtaac tatggatact catattcaac attgacagag ttaccaaatc
     7681 atgaaattgg attgatgttt gaaaaatttg attcatggtc tcgtaatgaa cttcatatga
     7741 aaaatgttgt accatatata acatttaaga ttgaagatct gaaaaagaat taaagctgaa
     7801 atttgaaaat atataaaaag aggataaaaa ttatggtaaa ttacggtatt gttggagctg
     7861 gatattttgg agctgattta gctcgctcaa tgaacaaaat tgaagatgca aaagtggttg
     7921 cggtatttga cccaaatcat ggagaagaag ttgctcaaga gttgggatca gatgtttgtg
     7981 caagtttaga tgaacttgta gcacgtgaag atattgattg tgtgatcgta gcttcaccta
     8041 gctaccttca ccgtgaacca gttgtgaaag ctgctcaaca tggcaaacac gtattttgtg
     8101 aaaagccaat tgcattgtct tatgaagatt gtaaagccat ggttgacgca tgtaaagaaa
     8161 ataatgttat ctttatggct ggtcacatca tgaacttctt taacggtgta caccatgcta
     8221 aagaattgat tactcaaggt aaaatcggta aagttcttta ttgccatgcc gctcgtacag
     8281 gttgggaaga acaacaacca actgtatcat ggaagaaact tcgttctcaa tctggaggac
     8341 atttgtacca tcatattcat gaattagatt gcattcagtt tatcatggga ggacttcctg
     8401 aaaaagcgac aatggtagga ggcaatgtat atcataaagg tgaaaacttt ggtgatgaag
     8461 atgatatgct cattgtaaac ttagaatact ctgatgatcg ttatgctgtt ttggaatatg
     8521 gtaatgcttt ccgttggggt gaacactacg tcttgattca aggaactgaa ggagctatca
     8581 aacttgattt gttcaatact ggaggtactc ttcgtgttaa aggtgaagga gaatcacact
     8641 tcttagttca tgaaactcaa gaggaagatg atgaccgtac agctatctat accggtcgtg
     8701 gtatggatgg agcaattgcg tacggtaaac caggagtacg ttgcccatta tggttgcaaa
     8761 catgtattga taaagaaatg gaatatctac atgacatcat taaaggtgga gaaattacag
     8821 aagaatatga aaaacttctc aatggtgtag ctgctttaga atcaatcgct accgctgatg
     8881 catgtacttt atcagttaaa gaagatcgaa aagtaagtct ttcagaaatc acaaatgctt
     8941 aacttttgta aaacagaata gtaaattctt atcattatat aatttctaaa gttctgtgat
     9001 acaactcatt gaataaagaa atagagatgg gactgggata atgcccagtc ccatttttta
     9061 tcaaaaagta atgagatcaa aaatgtggga gtgatgaaat gaagattata gggatcgata
     9121 ttggcggaac aacaattaaa gcagatttat acaatgagtt tggaacgagt ttaaatcagt
     9181 tcaaagagat agaaacaatt attgactatg atttgggaac gaaccagata ttaaatcagg
     9241 tctgtgattt aattggtgag tatattttaa atcattcaat tgatggtgtt gggatttcca
     9301 ctgctggagt tgttaatgct aatacaggag agatcatcta tacaggctat acaataccag
     9361 ggtatatcgg agtaaacttt actttcgaaa tagaaaaacg ttttgggttg tctacttttg
     9421 ttgaaaatga tgttaattgt gctgcattag gtgaattgtg gaagggacaa gccaaagata
     9481 agaaaaatgt agtaatggtt actattggaa caggtatagg aggcagtatt attgtcaatg
     9541 gacaaattgt taacggattt aactatactg ctggtgaagt aggctatatt cctgtaggta
     9601 attcggattg gcaaagcaaa gcctcaacaa ccgcattgat tcatttatat caaaagaaaa
     9661 gcttgaaaac taatcaaact gggcgtactt tctttactga tttaagagct ggagataaag
     9721 tcgctaaaga aacttttgaa atttttgtag aaaatctaac aaaaggttta ttaacgatta
     9781 cttatctact taatccagaa attctcatat taggaggtgg gatcctggct aaaaaggata
     9841 ttttgttacc tgaaattcaa agttctttag ctaaaaatgc aatggataat aggtttttgc
     9901 ctaaaaatct tgtggcagct acattaggaa atgaagctgg tcgtatagga gctgtaaaaa
     9961 atttcttaga tagaatttct aataaatagt atgaaagata aggaggtgtc acaatgacta
    10021 actctgtatt ttcgacaatg caagatattg agaatgttgc aaccgatatt ataaaatcat
    10081 atgataatga gatttatact tataaagctg tttcccaaga agaattggaa aaactagaaa
    10141 aaagttatga tgaaaaaagt cacgaagaat tagtttcaat agaaagcaat ttagaaatga
    10201 aacaacagaa ccttattgat gaggttaata aaacaatcaa ggaaaattat gaaaagattc
    10261 agtatatttc atcaagtagg agaggagaat ttgtagaaaa aattattggt agggtggtag
    10321 aaaaatatgg ctattagtca gatgaaaaga atttctctac tattttctaa aagtagtctt
    10381 gatgatgttt taaaaactat tcaagaacta gagtcagtgc agttccgtga tttaaaggtt
    10441 caggataact ggtcagaagc tctagaaaaa gatgaggttg tatttccaac tattcaaatt
    10501 tctcatactt ctaattccaa tcatggggtt attgaaggaa atgatgcctt gatttatttg
    10561 atgaatcaac aacaatattt agaagcaact gtagagaaat tacaagaata cctaccgaaa
    10621 gaaaacacgt ttaaattatt acagcaacct ccgataacga cctcttatga agaattagaa
    10681 aaatttggta aagctaatgt tgctgagggt gttcttaaaa aagtgaatcg tcaaataaac
    10741 agagttcatg aattagaaag acacattcaa agtaataatg aggaaataga gagattaata
    10801 aaatgggaaa aattagaaat tattcctgcg catttagaac aatttttttt ctgtaaagga
    10861 aaagtcggaa caattccaag gactgtagat aatcgcttat acaatagttt tttagaaaac
    10921 aatattgaag ttcaagaaat attttctaat gatagagagt acggtgttgt tgttttctat
    10981 cagtccagtt gctctattga ttttgatgaa tacttatttg aaccatttga ttattctaga
    11041 aaggaattac cgaagcagcg agtagtagat ttggatcaag aaaacatgca gttaataact
    11101 gaaaaagaga atattatcgc atcgttgcaa gattcaaaga ataatttgat agatttacaa
    11161 tggcaaattg actatatttt atctatctat gctcgtcaaa tctctaagaa taaccttttg
    11221 agcactccgc atctagttgc actagaagga tggatagaag aaactcgt
//
LOCUS       DAVBBS010000060        11187 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVBBS010000060 DAVBBS010000000
VERSION     DAVBBS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11187
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.060"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..947)
                     /locus_tag="VQU32_001759"
     CDS             complement(<1..947)
                     /locus_tag="VQU32_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2473170.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQVIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFILVTFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTI"
     gene            complement(1027..1890)
                     /locus_tag="VQU32_001760"
     CDS             complement(1027..1890)
                     /locus_tag="VQU32_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET2473171.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTSLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(1893..3794)
                     /locus_tag="VQU32_001761"
     CDS             complement(1893..3794)
                     /locus_tag="VQU32_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2473172.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNDLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENAAV
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINCVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
     gene            complement(4222..4497)
                     /locus_tag="VQU32_001762"
     CDS             complement(4222..4497)
                     /locus_tag="VQU32_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET2473173.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(4603..5442)
                     /locus_tag="VQU32_001763"
     CDS             complement(4603..5442)
                     /locus_tag="VQU32_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2473174.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVIVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRIGRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            5584..6432
                     /locus_tag="VQU32_001764"
     CDS             5584..6432
                     /locus_tag="VQU32_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET2473175.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(6525..7442)
                     /locus_tag="VQU32_001765"
     CDS             complement(6525..7442)
                     /locus_tag="VQU32_001765"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET2473176.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            7879..8084
                     /locus_tag="VQU32_001766"
                     /pseudo
     CDS             7879..8084
                     /locus_tag="VQU32_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(8395..8688)
                     /gene="rpmA"
                     /locus_tag="VQU32_001767"
     CDS             complement(8395..8688)
                     /gene="rpmA"
                     /locus_tag="VQU32_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET2473177.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(8705..9049)
                     /locus_tag="VQU32_001768"
     CDS             complement(8705..9049)
                     /locus_tag="VQU32_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HET2473178.1"
                     /translation="MIQAVFERAEDGELRGAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(9065..9379)
                     /gene="rplU"
                     /locus_tag="VQU32_001769"
     CDS             complement(9065..9379)
                     /gene="rplU"
                     /locus_tag="VQU32_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET2473179.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYIEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(9415..9502)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(9597..10196)
                     /locus_tag="VQU32_001770"
     CDS             complement(9597..10196)
                     /locus_tag="VQU32_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HET2473180.1"
                     /translation="MTFCAGYVDAYTFIVRGNTLVAGQAGNVVFLSVELIQNNVSDVR
                     DKLLTLITFIMGVFLLTIYKEKLRIVKKPILSLIPLAILSIIIGFVPQTVDNIYLVQP
                     LAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLAFGDYFRTKHTPFLREGFIFVSLL
                     SSFVLGDVFSAYLTIFYHEKTILGVPIMMSIFYLSMLFA"
     gene            10666..10965
                     /locus_tag="VQU32_001771"
     CDS             10666..10965
                     /locus_tag="VQU32_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET2473181.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT         3535 a         2316 c         1863 g         3473 t
ORIGIN      
        1 aaaatcgtca taaacaattc acaaactaaa tttccagaaa aggaaagaaa agttggaagt
       61 ggtaatccca tcataaaaac tccgacacct gtcaaagcca gtaaaatcaa aagattataa
      121 atattagctt taattttact agctagaaga gccccaatga tggaaccaat agcccccata
      181 gttaaaatac ttgcataggc tccttctgac ccgtaaagct gattcgaaaa gggaagtaga
      241 aattcaaaag ctgcaaaaaa gaaattaacg ctggaagcta ccagcaaaag gaagaaaatt
      301 tcttgctgat gccagatata gtgtaaccca tccttgatat ctacaaaaat atctctccca
      361 gtaaaagcct ttttctcttg aacttttgct tcctcttttg gaaggaaagt cactagaata
      421 aaagcaatga aaaaagtcag cgagtctagc agtagcgtca tatggagact tgcaaactgt
      481 aaaacaagga aggaaagaac aggagagcta acacctacaa cctgcaaaac cagctctaag
      541 cgagaattat agatcacaat ctcatctttc tccaccactt cagttatgat agctttattg
      601 gctgtgcgag aaaaggcaaa agcaataacc tgcacaatgt tagcaacaat caaagcgcca
      661 atcatccagc tatcattcct tatgaaagaa atagccagac aaagaatccc acaaacaaga
      721 tctgccgtca ttaaaatctt acgacgagaa aaacggtctg aaataactcc gccaaaggga
      781 ttgacgagaa tagatgtgac gagctcagaa atctgataca ttcctaaaac tgtctgtcct
      841 atagtcccca tagaagccaa ccagacacta tttccataat catagagcat atttcccatt
      901 ttattgatag ccccacggct aatcaactgc actgcatagc gattcatatt aaagctcctc
      961 tcaaattttg aaactattgt atcaaaaccg aaaggagctt tttatttttt cccttatttg
     1021 ggaaaattaa cttttgacaa atttttcgta gtgttcctga taataggcta cttgctctgg
     1081 aagacctaac acatcaaaaa tatgcatggc ctcttgcatc tgcttacagc cttctttaca
     1141 ctgtcctttt tgatataagg caaaaccttt taaataatgg aaaacattac gctcataaag
     1201 cttaatacct ttgtcaataa tcttctctgt ataagcctca aaatagttgg cattataaaa
     1261 agaagaatgc tctaaacaat gctggtaaca attgagggcc aaaatcaaca ctaatctctt
     1321 atggcgacta atctcttggt aaaattcctc cctctccata acttctctac caatccgagt
     1381 gacatagtct acatcgtaga aactatagag gttaccgaaa agaatcaact catacatggt
     1441 ccattcttct gttttgaaga gataatctgc taccttatcc aaatcatcct gcttcatatc
     1501 ataactcgca tctctttgac aaatcagacc ttgtagcaaa atccagttca gctcaaaata
     1561 aagggaagtc gtcgaactct tagacttttc aagttgttct ctttgaagct tttgaaaacc
     1621 tgcaatatcg tttgaatagt aaagtgggat aatctgtgcc atcatagaca catgttcatg
     1681 attatgaaaa ttccttgcct tatccatgaa attttcgatt gttacatgaa tgttatccaa
     1741 aatctcaaag aaacgggaga ctgccaggtc agactcccca agctcaaagc gagataactg
     1801 agaggtagag caggattcgc ctgctgcttc ctttaaagaa taatttccac ttgttcgaaa
     1861 ttcacgaaat acttttccaa gatgttccat ctttacacct gatctgataa ttcttcccac
     1921 tcaagcatag cttcttcctg acgatggctg attttatcca gctcagcctg taattccatg
     1981 agtttgtcgg catcgtttgt ttccaacatt tgttcagaaa tggcttggct ttgactttct
     2041 agctcttcaa tttcagcttc tagactttcg atttgtcgca tgagtttgcg aacttctttt
     2101 tgactttctt tctgggcctg atagtcattg actggacttg cttcctttgc ttgattgcta
     2161 gttgaagctt cctcagtctg actcatttct gctgttgctt tcttctcaac atagtagtcg
     2221 taatctccaa ggtagagagt tgaaccattc tcagacaatt ccaaaacatg agttgccaca
     2281 caattgataa agtaacgatc atgactgaca aaaagcaagg taccatcaaa gtcaatcaag
     2341 gcattttcta gcacttcctt actatcaata tccaagtggt tggtcggctc atccagaatc
     2401 aaaaagttat tgttttccat agacaattta gctaaaagca aacgagcttt ttcgccacca
     2461 gatagcatgc cgactgattt tttaacatca tctcctgaga aaaggaaggc tccaagacgg
     2521 ttgcggattt caacttctgg tgtcaatttg aaatcattcc agagttcatc cagcaccgta
     2581 ttacttggtg tcagcttgct ttgggtttgg tcatagtaac caacctcaac attagcgcca
     2641 aagcgctttt ctcccttgat aaacggaatt tgatccacga ttgatttgat aaaggttgat
     2701 tttccgatac cattgggacc aacgatagca acagcattca tcttacgaag gtctaggtta
     2761 atcggttgtg acaggacttc cccatcatag ccaacagccg cattttcaac agtcaaaaca
     2821 acattgcccg acgttttttc agactggaag gtcatgttgg ctgctttctt gccagcttcc
     2881 ggcttgtcca aacgctccat tttttctagt tgtttacggc gagattgagc acgtttggtc
     2941 gttgaagcac gaactagatt gcgattgaca aagtcttcca gagcagcgat ttccttctgt
     3001 tgcttttcat agttttttgc ctcagtagct agcttttgct ccttcaactc gacaaaacga
     3061 gagtaattcc ccacatagcg atccaaggaa tgcttggtca aatctagcgt aattgtcgca
     3121 accttgtcca agaaataacg gtcgtggctg acgataatga gggcaccgct atagtttacc
     3181 aagtaattct ctagccagga gatggtttca atatccaagt ggttagttgg ctcgtccaag
     3241 accaagagat tgggcttttc aaggagcatt ttagctagag ccaaacgagt attttgacca
     3301 cctgaaagtt cagctatttt catctgccac atagactcgt caaacttgaa tccattcaaa
     3361 atcgctcgaa tatcagcttc ataggtaaag ccacctgctt ggcgaaaatt ctcagataag
     3421 cggtcataat ctgacatcag tttatccaaa tcctcaccag acttttcacc catctccagc
     3481 tccatctgac gcagttgtct ctccgtccga cgcaaatcat taaagacatg aagcatttca
     3541 tcgtagatgg tattttcaga ctcaaaacgg ctatcttggg ctaggtaaga cagagaaata
     3601 tcttttttct tattgatttc tccgctagtt ggctcctctt ctccaactaa aatcttcaaa
     3661 agagtagact tacctgcacc atttttccca acaagagcaa tccgatctcg ttcatcaacc
     3721 tgcaggttga tattatcgaa aagaacctct cctgcaaaag aacgttcaat tttattagct
     3781 tgtaaaataa tcataagagt agtatagcat gtttccctaa ggcattcaag ataatcgtaa
     3841 gtcttttagt acaactttta taacataaaa taaactaaat tatgtatatt ttatattaga
     3901 ttccttcact atcttgttgg attttctaac cagctaatct tgtttcaaat agttatcgca
     3961 caagcctatt atttaattct tttcattatt tacgtacgcg tatagcagat tgaaataaga
     4021 tgagaacaaa tcgattggga aagtaaaatt aatttctata aatgttttag caattgtttc
     4081 gtactatttt agattcagtc tactatgtac aatattttcg gaacattcaa ctttttaact
     4141 ctatttatta ctagatttca taattaaaaa gcctactgac caagctagaa agcttgatac
     4201 aataggcttt ttaaagactg attatttaac agcgtcttta agagctttac cagctttgaa
     4261 tgctggtact ttagaagctg caattgtcat ttctttacca gtttgtgggt tgcgaccttt
     4321 acgttctgca cgctcacgaa cttcaaagtt accaaaaccg atcaattgaa ctttttcacc
     4381 agctgcaaga tagtcagcta ctgctgcaaa tacagcttca actgctgctg ctgagtcttt
     4441 cttagtcaat tctgtagctt ctgctacttt agcgatcaaa tcttgtttgt ttgccatgtt
     4501 aatgattcct ccagataatt tctaattaac aaatataatc atatcctaaa atccctttta
     4561 ggtcaagtta aaaacgctat ttcttccaat tttatttatt ttttaggagt ggtaacgtat
     4621 caaaatagcc caagcgttct cacccgtgtg agtttgaata atggaacccg tttccaaaac
     4681 agaaattggc ttttcaacat aagcttgtaa gctttctttc atctcttttg cccaatcatc
     4741 actaccagaa tatgaaattc caatctctgc tacagcacgt tcagaaagcg atgttatcaa
     4801 ctcatctaac cattttttaa atgttttagt tccacgacct ttaaccattg gctgcaattc
     4861 atggtctttc atttgcatga caacacggat attgagaagg gagctcaaca agccagttac
     4921 acggccaatt cgtccacctt tgacaagatt ttccaaagtt gaaacaccaa tataaagctc
     4981 tgtatggttt ttaacctctt ctacatgaga taaaattgcc tccatatctt taccttcttg
     5041 agctaacttc gcagcctcaa caacttggaa tttcagggct tggtcagtga aggaactatc
     5101 aacaacaatc acatctgcag tagataggct agcaccttgg cgtgctgctt ctaccgtacc
     5161 cgaaagagca tgggacatat gaatagcaag aatctggcca ccatctttgc ataggtcttc
     5221 aaaaatctca gcaaagacac ctacaggtgg ctgacttgtt ttcggaagat tcttactttc
     5281 ttgcatcaac tgaagaaatt taccttcttc tttcaaatcc gcatcagaat aaacaacatt
     5341 atcaatcatt acagataatg gaacaattgt aatatctaat tgctttacta gttcaggttc
     5401 aatagtaaca gatgaatcgg ttacaatctt aatttttgtc atagtatcaa tctttctatt
     5461 ttaggattca gattggtttc cttacttcta attatatcaa aaaaaagatt aaaaatccta
     5521 atggagtcaa tcaaattttc cgtaaaattt gatataatca acttataaga aaagaggtgt
     5581 cctatgatta aaaaaattta ccccattttt accattttac taggtgctgc tatttatgct
     5641 tttggactga cttattttgt agttccccat catctctttg aaggaggggc gacaggtatt
     5701 accctcatca cattttatct ttttaaaatc cctgtttccc tcatgaacct gctgattaat
     5761 attccccttt tcatcctagc ttggaagatc tttggagcca aatccctcta ttctagttta
     5821 ctaggaacct tagctttgtc cggctggtta gctttttttg agcatattcc ccttcatatt
     5881 gatcttcaag gtgatttact aataacagcc cttatagcgg gaatcctatt gggaattggc
     5941 cttggaatta tttttaatgc tggaggtaca actggcggaa ctgatattct agctcgtatt
     6001 ctcaacaaat acactcatat atccatagga aaactgctct ttatcttaga tttttgtatt
     6061 ctcatgttga ttctcctaat cttcaaggat ttgagattgg tttcctacac gcttttgttt
     6121 gattttattg tttctcgtgt tattgatttg attggtgaag gaggatatgc cggcaaaggc
     6181 tttatgatta tcacaaaacg tcctgaccaa cttgctaagg cgattaatga tgacctcgga
     6241 agaggtgtta cttttatttc tggtcaaggc tactatagta aagaaaattt gaaaatcatc
     6301 tactgtattg tcggaagaaa tgaaattgtg aaaacgaagg aaatgattca tcgaatcgat
     6361 cctcaagcct ttataactat tacagaagcc catgaaatcc taggagaagg cttcaccttt
     6421 gaaaaagaat aaaaagaggt aatgtcgtga cctcaaaagt tagactaaat catctatctt
     6481 ttgggttaca gacaacctct tttttgtttt atttactcaa gctcttaaga ccaattccga
     6541 gttacttctt catcagcctt taactgatcc actaattggt caactgagtc aaatttggtc
     6601 atatcacgaa tgcgatcaag ccaataaacc atgacggttt ccccataaat atcttgatta
     6661 aaatcaaaaa tattgacttc aaaacgtgct tcttctccat caaaggtcac atttttcccg
     6721 acactagcca tagcacgata cttctgtctt tgaatctcaa catcaacgac ataaacgcca
     6781 tctgctggca tataagtacg gtctaaaagc actaaattcg ctgtcggata accaattgta
     6841 cgaccacgag cattaccatg aaccaccata cctcttgatg gaagcggtgc ccccaaaagt
     6901 tttcctgctt ctttcacatt tccatctaaa atagcttgac ggatacgagt tgaactaatc
     6961 tttcctttct catcttctac aggtggaaca atgataactt ctccatcaaa gtaatccttt
     7021 aaatcttctg ctgttttttt gtcagaacca aatgtataat caaaacctgc aacaataatt
     7081 ttggcattca tagccttgat ataagttgca aagaattctt gtgcagtgag actagcgaat
     7141 tgactactaa aatcaaggag atataattct tctacacctt cgcgctttaa ttttctttca
     7201 cgttcagcag ggttcaaaat atgcaaaaac aaatccggat gataaggctc taaagcgatc
     7261 tttggagatt cattaaaggt cataacgacg ataggtaata aatcctttct cgcagccttg
     7321 ttggcaacac gaaataattc ttgatgcccc ttatgtatgc catcaaaata gccgagaaca
     7381 acgactgaat cagatggtgt gccaatatct ttttggtttt ttataggaat agtaataatc
     7441 ataaaataat tatatcatag cgatagctat ttctggaaca gaaaatctga aatgttgttt
     7501 ttttcacatg aagtgtacct gttttcaaaa agcactttat tctatcgttg cttaactatg
     7561 aactttgcaa tattcttctc aaaaacttgt aggacatctt caaaattttg caaggagtga
     7621 ttagacttgt tcggtaacca taaagtgtca tactatgtta tgtatgaaaa agcaatgcaa
     7681 ctaactcctg agaactttaa attactaatt ggtgccgaaa aggtagaatt tagaatcgag
     7741 gtacacctat ggctgtaaaa tttacaaaat gagacaactt gggcaagatg tttgaagaat
     7801 ttcctaaact ccctgatttg aagcaagtca ctttccctga tgacaaagaa aaaagccaaa
     7861 acagcaaaga aaaactagat gactgctttc caacaactcc catctagtgt gcttcagact
     7921 gggctatttt tctctccatc tgttagcttg gattctcaga ccgtttcagc taaagaatat
     7981 cttttccctt atcagaagga acggctcaaa ccattcagac aagtgaaggg acgacaagcc
     8041 aatatttgaa accagatagc agttcttata gtcaattgaa ataaaatctg aagaaatcga
     8101 gtaggaaact catatcaatt tttaacagtg ttctattcca gattcatact caatgaaaat
     8161 taaagtgcaa actaggaagt tagccgcagg ctgtacttga gtacggcaag gtgaaactga
     8221 cgtggtttga atttgatttt cgaagagtat caatccacta tacatattaa aaagtatgga
     8281 tatcaaaaca aaaaaacagg ctctccgaaa actcggaaag ccttatttta agctgtttct
     8341 agcttcctca cctttacatt tcaaggttcg ggataaaagg ttcaatggac ctttttattt
     8401 agcgattggg taaacagaaa cttgtttttt atcgcgtcct ttacgttcaa agcgtactac
     8461 gccttcaact ttagcgaaca aagtatcgtc tccaccacga ccaacgttta cacctggata
     8521 gatgtgtgta ccacgttgac ggtaaaggat tgatccacct gttacagttt gtccgtcagc
     8581 tgctttagct ccaagacgtt ttgcttgtga atcacgtccg tttgatgtag aacctccacc
     8641 ttttttgtgg gcgaaaagtt gcaagttgtt aagagtcatt tttaacataa tgttttcctc
     8701 cgtgttagtt ttctgtgata actctggttt ggacgaactc agaatagttc tccgataagt
     8761 ttgccatacc taagaaaaat gattcaaaga ataactgggt catttctctc tggtgtgaag
     8821 gaagatcttt tggtatttca accatcagat agccaccttc atcttcgttt aattctagga
     8881 ttggttcata gcctgcaaat ttctcaatag aattgataaa gttaatggca agcgtagaaa
     8941 ccgatgcaca cacgacatct aagccgtatt cgccactctc ggcgtgtcca gtaatttccg
     9001 cacccctcag ctcgccatct tcggctctct caaagactgc ttgtatcatg tgttctcctt
     9061 aaaattaagc gttaattgcg ttgatgacaa cttttgtata tggttgacgg tgaccttgtt
     9121 tacggtggct acctttttta ggtttgtact tgtaagtaac aactttcttt tgttttcctt
     9181 gtttttcaac agttccaact acagtagctc cagcaacaag tggagttccg acaacagtgt
     9241 tttcaccacc aacaagaaca acttcgttaa aagtaacttc ttgaccagct tcaacgttca
     9301 atttttcaat gtaaactgct tgaccaactt caactttaac ttgttttccg ccagttttga
     9361 taattgcgta tgtgctcatt atgcacctcc tatgattttt atgggtttcc ccgatttttt
     9421 gtgaagactc gcctagcatc gtgggacaaa ccacttaaac ttgaataatc aagcaacgat
     9481 gttcgtgcgg ttgcacagga tcgtgcatag tcaactcttc aagtatagca tatctcctat
     9541 tttcttacaa gtaataacac ctaaaatcaa gctttttctt ttactttttt ctgccactag
     9601 gcaaaaagca tactgagata aaaaatgctc atcataatag gaacaccaag aatggtcttt
     9661 tcatgataga aaatcgtcaa ataggctgaa aagacatcgc caaggacaaa actactaagc
     9721 aggctaacaa atatgaaccc ttcacgcaaa aaaggagtgt gcttagttcg gaaataatct
     9781 ccaaaagcta gcattgttcg tttgatattc cctgtcataa aggcattatt gtaggcaata
     9841 cccgaaacct caccaaaagc agttgtcacc agtcccatac agaaggccaa gggctgcact
     9901 agatagatat tatctacagt ttgtggcaca aaaccaataa ttattgatag gattgctaaa
     9961 ggaatcaagg acagaatagg ttttttcaca attctcaatt tttccttata aatcgttaat
    10021 aaaaagactc ccattataaa cgttattaag gttaaaagct tatccctaac atccgaaaca
    10081 ttattttgta ttagctctac cgaaagaaag acaacatttc cagcttgtcc agctacaagg
    10141 gtattccctc gaacaataaa agtgtaagca tccacatatc cagcacaaaa tgtcaaaaaa
    10201 gtgctaacct tttagactga cgtgatattt ttcttatagg taataacctc attttccctc
    10261 ccattgtatt ttctcttaga aatattgtac catttctttc taaaaaatcg tagactacca
    10321 tttagatttt actattagca taaaaataat aatagacaac tatttatcca aaaatagata
    10381 gaggtaacat gtttgcaaac aaagcatacg aacctttagt aaaatcattt ccatgaaact
    10441 agaatagagc ccctcttagc aaaaatcatt attttaattt atttctaatc actccttgac
    10501 ataaataact ctcaccaata aaagactatg tcttaaaaaa atggtataat aaaatcaata
    10561 cctgggcttg atggctatgc tactaataac aattaggaga gaaaatcagg cacttgttaa
    10621 caacaaggat tatccccttg agatgaaagg aactttagaa atcttatgat gaacatgcaa
    10681 aacatgatgc gtcaagcaca aaaacttcaa aaacaaatgg aacaaagcca agctgaactt
    10741 gctgctatgc aatttgttgg caaatctgct caagatcttg tccaagcgac cttaactggc
    10801 gataagaaag ttgtcagcat tgatttcaat ccagctgtcg ttgacccaga ggaccttgag
    10861 actctttctg atatgaccgt tcaagccatc aactctgctc ttgaacaaat cgatgaaact
    10921 actaagaaaa aactgggtgc tttcgctggg aaattgccat tctaaaaaca aggagctaga
    10981 acaatacttg tcgataacaa aggctaagaa aggtgcaaaa attataaaaa acgcatagta
    11041 tcaagcaacc taaaactttg ggctctataa tatttgtagt gggtaaatcc actatagata
    11101 ttatggagcc tattttattg tagaaaaaaa tcccataaga tctataatga aaagcgacca
    11161 aacaactcat tagaaagatt catatgg
//
LOCUS       DAVBBS010000061        11086 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVBBS010000061 DAVBBS010000000
VERSION     DAVBBS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11086
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.061"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(6..890)
                     /locus_tag="VQU32_001772"
     CDS             complement(6..890)
                     /locus_tag="VQU32_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HET2473182.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDMIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(894..1280)
                     /locus_tag="VQU32_001773"
     CDS             complement(894..1280)
                     /locus_tag="VQU32_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HET2473183.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLISLMLEEQILTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(1273..2616)
                     /locus_tag="VQU32_001774"
     CDS             complement(1273..2616)
                     /locus_tag="VQU32_001774"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET2473184.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYEVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEGLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(2698..3582)
                     /locus_tag="VQU32_001775"
     CDS             complement(2698..3582)
                     /locus_tag="VQU32_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2473185.1"
                     /translation="MIQVRNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHIEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(3594..4211)
                     /gene="cysE"
                     /locus_tag="VQU32_001776"
     CDS             complement(3594..4211)
                     /gene="cysE"
                     /locus_tag="VQU32_001776"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HET2473186.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFIW
                     KHGFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(4227..6440)
                     /gene="pnp"
                     /locus_tag="VQU32_001777"
     CDS             complement(4227..6440)
                     /gene="pnp"
                     /locus_tag="VQU32_001777"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET2473187.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDDQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRLELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            complement(7125..7991)
                     /gene="metF"
                     /locus_tag="VQU32_001778"
     CDS             complement(7125..7991)
                     /gene="metF"
                     /locus_tag="VQU32_001778"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HET2473188.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(8055..10304)
                     /gene="metE"
                     /locus_tag="VQU32_001779"
     CDS             complement(8055..10304)
                     /gene="metE"
                     /locus_tag="VQU32_001779"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HET2473189.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVASDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(10565..10831)
                     /locus_tag="VQU32_001780"
     CDS             complement(10565..10831)
                     /locus_tag="VQU32_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2473190.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGVSQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
     gene            complement(10915..11086)
                     /locus_tag="VQU32_001781"
                     /pseudo
     CDS             complement(10915..11086)
                     /locus_tag="VQU32_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         3095 a         2540 c         2194 g         3257 t
ORIGIN      
        1 cttctttaca aaccaactat agttgacaaa gaacctaaaa tcaattgata acacaaggtc
       61 aggtcggtca actctttcaa ctgaagccct gtcaactctt cccatttatc aatcttgtat
      121 tggagagaat tgcggtgcag atagagttgc tgggctgttt tagtgagaac agcactattt
      181 tcccaaagag agagaatgat ttcctgaatc tgatcttgat ccaaaatcat ctggtgtaga
      241 cattctttaa ttggcttcaa gtccacgagt ctttctccca tactccaaag atagagctga
      301 gaaaaagtat gaacaccttg gtgaccctga cgccaccatg tcttgaacaa atcccgctca
      361 gctttgatta agtctgatag ggcttgatgt cctgtctgag accaaacctg acccaacatg
      421 atagaaagac gaagtccaaa gtcatactca actgcttcaa tcatatcact taaaatatct
      481 cttacagaag tgtatttgtc ttgttgaagc acgaaaacat aatcctgagc tccgacctgt
      541 aacactgtct gacaattcgg aaaaagagtc cgcatcatat ctagccaaga agccagattt
      601 tcctgctgaa aataagaaag atggcaataa accaactgaa tctttttaaa aacttgcggt
      661 gcctgtccct tgccttcaac cagataggaa taccaagggt ttagcgaacg agcctgctcc
      721 tgctgggtca aaagggcaac caactgcttt tcacgctcgc tgagcccagc ttcctccagc
      781 aaaatccact gctgagaagc taaaggaagc gtgagatagc cctctttctc tactggttgg
      841 tctgaaatcc gagcctcagg aaaccagtct tgtagttctt ttgccctcat gttctagccc
      901 tccacttttt ggatgcacca tgaaaccaaa ctctcaagac gttccagatt ctcagtcata
      961 tggagatagc ccataactgc ttcaaatccc gtggacatac gataagtcac gacatctgca
     1021 tttttagcct ttgtgtgact attggtattg cggccacgtt tgtagatttc ttcttctttt
     1081 tccgttagga tctgctcctc caacatgaga gaaatcaggc gagcttgagc cttggctgac
     1141 acgtacttgg tcgcctcttg gtggagttta ttgggcttgg tcataccttt gaggatgagg
     1201 tggcggcgaa tatacataga atacaccgca tctccctcaa aggctagcgc aatcccgtta
     1261 atgagattga catcaatcac gtgtccacct cactccatcc ttggtatcaa ggagcttaat
     1321 tccttgagta accaattggt cacggatttg gtctgctgtc gcaaagtcac gattggcacg
     1381 cgcctcttgg cgtttttgaa tcaagtcttc aatctctgca tccaaaactt cctcaacaaa
     1441 gacaattcca aaaatttcta acatatctgc aagagcttgc ttgacacttg catcatagtt
     1501 ccctgagttg atccatttgg ccatttcaaa gacaactgtg ataccgttgg cagcattaaa
     1561 atcttcatcc atagctgcta caaacttatc tttaaagttt tgtaactctt gggcatccac
     1621 gtttcctgta aatggttgtt cgtaagtatt cttcagatac ttgagattgg tctcggcatc
     1681 gcgaactgcc ttttccgtga agttgatagg cttacggtag tgctgggtcg caaagaagaa
     1741 acgaagtact tgcccatcaa gagttttaag ggcatcgtgt accgtaatga agttacccaa
     1801 ggacttagac attttgacat tgtcgatatt gacaaagcca ttgtgcatcc agtagttagc
     1861 aaaagccttg cctgttttag cttcagattg ggcaatttca ttggtgtggt gtggaaactc
     1921 taggtcagct ccaccaccgt gaatatcaat ggtatcacct aaaatctctg tcgacatgac
     1981 tgaacactca atatgccaac ccggacgtcc aggtccccaa ggactatccc aagaaatctc
     2041 acctggtttg gaagatttcc atagagcaaa gtctacagga ttttccttac gagccgtttc
     2101 ttcatcggta cgacctgaag cacctagctc caaatcttcc aaggttttat tagccaattt
     2161 agcatagttg tgggattttt ctacacggaa atagacatcc ccttgactct cataggcaaa
     2221 gcctttctcg atcaagcctt ccacaaaacg gatgatgtct gccataaact ccactacacg
     2281 cggatggcga gtcgcaggtt tcacgcccaa ggccgtcaca tcctcacgaa aggcagcgat
     2341 gtacttatcc gcaacctcct gaggcgtgat gccttcttcc ctggcacggt tgataatctt
     2401 atcatccaca tccgtaaaat tggaaatata ggcaacctcg tacccacgat actcaaaata
     2461 gcgacgaatg gtatcaaaag ctaccgttga acgagcattc cctacatgaa tatagttgta
     2521 caccgttggc ccacaaacat acatcttgat cttgccgtcc tcaatcggga caaattctcg
     2581 caaatcacga gacatggtgt catagatttt aatcataaat cataatcaag aaagctgaaa
     2641 tccaagaaca attagtttca tcactaaaag ttcaagtaaa tttcagtccg aatatctcta
     2701 cacttcggaa tcccttgctc ctttctcatt cagataaacc acctgagtct gtttgacaaa
     2761 gccaattttt tcatacaaac gtttggcacc tacattgcta tcttccactg caatctgaaa
     2821 ttccttgtca ttttgctcaa ttagttggtt gacgagggat tttgctaagt agcttccata
     2881 gcctttccca cgttcaggtt ccgatattgc taaaccgtag aagtaattcg tattagtcga
     2941 taaatctacc gtgcaagtcc caataacctg accgtctttt aacaaaatat atagtcggct
     3001 ttctggatcc ttcagagctt cagcgacata tctatccaca acttctctcg attcatgttc
     3061 ctctgaaaat gcctgaaatt ttaattgact aatttgatcc tgatacgaac tatctgctaa
     3121 caaaacttca atatgggaaa catttgctaa cggataaggt cttctatcct tacctaacca
     3181 agtttctgtc tcttcatcct cgattagtcc ccagttactg gcaaagtcag gatgattctc
     3241 taaaaaaata cgttctgtct gaaaagtgac tgatcgaatg gggaaagaag ctgtttctct
     3301 ctcaaaacta gtaaacaatg cacgcgcaat cccctgacgg cgatgacctg gatgaaccag
     3361 tatcgtcact tctacatctt ggtcatctgc atagacagtt aataaaccaa caagttcgcc
     3421 tttttcataa taaaggaaaa aggcgggcat gtttgggtca aaattaagca tgttagagag
     3481 atagggatcg cgataggtac cgtcatagtt ttggcaacag ttaattactt ttttcgcctc
     3541 agatagctcc tcttggctta acttgtttct tacttgaatc atataggtat cctctacaaa
     3601 ccagacgatc tgtgactggc atctttagcc tgctcgagtt tattgacata atactctcgt
     3661 ttttcttcga cttcgtgaat gacaggctca tctttcttac catgaagacg gacaatcttg
     3721 gccggaatac cgacaaccgt cacatcacta ggtacatctg ctacgacaac tgctgcagca
     3781 ccgaccttgg cattttcacc aatttccaca ggtccgataa cttgggcatg ggctgatatg
     3841 agggctccct ttcgtacagt cggatggcgt ttgccacagt ctttccctgt tcccccaaga
     3901 gtcactccgt gatagagaag aacgcctttt tcaacaatcg ctgtctctcc aatcaccaga
     3961 ccagaaccat ggtcaataaa gacacctgaa tcaatctggg ctcctggatg aatctcaatc
     4021 tgagtccaaa agcgccaaaa ctgactgtgc atacgagcta agagtttgaa gccgtgcttc
     4081 cagataaaat gcgagagacg gtgggccgcc aaggccttga cacctggata agtcagcaaa
     4141 acctccaaag tggtgcgggc cgctggatca ttttctttta cgatatcaat ggtttcgcgc
     4201 caccacccca tacatttctc cttttcttat tctgaatctt ttggtgtttc tgtaaattct
     4261 ttcttaggtt tgtgatcctt gtgatgacgt gggcggtgag gacgctcaga cttttcacct
     4321 ttttcatcat gctcaggttt tggcggacga ggtagaagag ccttcataga ggcatcgata
     4381 cggccttttt catcaatctt gataacctta acatcaactt catccccgat ttctaccaaa
     4441 tcctctacac gattggtacg agtccaagcc atctcagaga tatgaacaag ggcatctgtc
     4501 ttatcaaaga ggttaacaaa ggcaccaaat ttctcgatac gaacgacttt agcacggtaa
     4561 acttcatcca ctttggcttc acgaaccaaa ccagcaataa tttctttggc acggttaata
     4621 gcatcttggt cactagagta gatagacaca tttccttctt cgtctatatc aatcttaaca
     4681 cctgtttcag cgataatctt gtcgatggtt tctccaccct taccgatgac aatcttaatc
     4741 ttgtccacat caatcttgat cgtatcaatt ttcggagcag ttggagccaa ttctagacga
     4801 acttctggaa tggttgcttc aatgacatca aggatttcaa aacgcgcttt cttggcttga
     4861 gcaagagcct ccgtcaagat ttctgcagta atcccttgaa tcttgatatc catttgaagg
     4921 gctgtaatcc catcacgagt acctgcaacc ttgaagtcca tatctccaaa gtgatcttcc
     4981 aaaccttgga tatctgtcaa tactgtgtag ttatttccat ctgagataag tcccatagca
     5041 ataccagcta ctggcgcctt gattggcaca ccaccagcca taagggcaag agttcccgca
     5101 cagatagaag cttgagatga agaaccgttt gattccaaaa cttctgctac tagacggata
     5161 gcgtatggga attcttccaa gcttggcaag acttgagcaa gagcacgctc accaagggca
     5221 ccgtgaccga tttcacgacg acctggcgca ccgtaacgac ctgtttcccc tacagaatat
     5281 tgagggaagt tatagtggtg cataaagcgt ttcttgtact ctggatccaa accatcaatg
     5341 atttgagttt ctcccatcgg agccaaggtc aagactgaaa gagcttgagt ttgcccacga
     5401 gtaaagagac ctgaaccatg tacacgagga aggaagtcaa caaccgcatc caaaggacgg
     5461 atttcatcga ccttacgacc atcaggacgc accttgtctt ctgtaattaa acgtcgcact
     5521 tctgcgtgtt ccatttgttc caagatttca gccacatcac gcataatacg gtcaaattct
     5581 tcgtggtccg catatttttc ttcgtaaacg gcagtcactt ggtctttcac tgcttgagtt
     5641 gcagcttcac gggccaattt ctcttctact tgaactgcct tttggaggtc actgttgtag
     5701 gctgcaatga tttcagcttg caattcagca tccacgtgaa gcaattccac ttctgctttt
     5761 tctttaccga cagcagcaac gatttcttct tggaaggcaa tcaattcttt gacagcttcg
     5821 tgccctttaa gaagcgcttc caacatgatt tcttctgaca attctttggc accagactct
     5881 accatgttga tagcgtgctt agttccagct actgtcaatt caagaagaga ttgctctgct
     5941 tgttcttgac ttgggttgat gatgatttgg tcatctacat atcccacttg taccccagca
     6001 attggtccgt caaatggaat atctgaaata gacagtgcca aagatgaacc aaacatagca
     6061 gccattggtg cagatgcatt ttcatcataa gaaagcactg tattgatgac ttggacttca
     6121 ttacggaaac cttccgcaaa cataggacga atcggacggt caatcaaacg cgctgtcaag
     6181 gtcgcatctg ttgaaggacg tccttcacgt ttcataaagc caccaggaaa cttcccagcc
     6241 gcatacattt tttcttcgta gttgacttgg agtgggaaaa aatccccagt tgccattttc
     6301 ttagacataa cggcagcagt caagacagtt gactcaccgt aacgtacgac aacagagcca
     6361 tttgcttgct tagcaacctg accagtctct acaattaact cacgacccgc aaaagtcgtt
     6421 tgaaacactt gttttgccat tttaatcccc tttggattga tgaaattata cgccttgcct
     6481 acaaagatca agataccaag gacgtcaaaa gcaaagtaaa aataggaaac tgacgaagtc
     6541 ttcgatgaag acaagacagt ttatcttttt tacacagctt ttcggccgtg ttcaattaca
     6601 caagatattt tggacggttc ggcttgccga acatttctgt agaaaaatag gaaggtgacg
     6661 tcgcactcga cgagtgctag gaagcttatc ttttttccta agaaatgaga ccaaaattca
     6721 agtcatcaag ataccaagcc gtcaagcaac tcaaaggaag ataggaaatc gaacgacgga
     6781 gcgactgctc ctaggcagat ttatcttttt ccacagagtt gtaggcgtgt tcagttttca
     6841 agatacatca ttagaaaggt ttaatattaa agtatctaaa cctttcacgc taatcgctat
     6901 cgggcgatta gctaaatgct ttactaactc tttcgtcaaa taacatcgat ttgactcact
     6961 cgtgtcgtta aatcttacag tttaaatgca ttgtattatt taataccttc atctttgtat
     7021 caagtacgta cagaatctat tttatcatat ttttcttaaa aagtgaggtc tttaccatta
     7081 aaaaggaacc attcccctca cctgagaaga atggtttgct tttattatcc tagagactgg
     7141 tgattaaaca aggcatgggt tgcttgatgg atgtattttg ctgtatcagc attattcatc
     7201 gtatagagat gcacaccggc aacatcctga gttaccaagt ccacgatttg gtccactgca
     7261 taggcaagtc ctgctgctct gagcgactca gggtcatgct catacttgtc taagatggct
     7321 ttaaatttgc gtggaagatg gatattctca caagtcttca agagtcggag agcctgattt
     7381 cgattcagaa ttggcataat tcctgcatga ataggaacat caatcccagc caagatacac
     7441 ttgtcctgaa aatcatagaa gcgctcattg tcaaagaaga gctgagttac gaggctcgaa
     7501 cagcctgcat ccactttctt cttaagattt tgaatatctg aaatctgatt tggcgaatct
     7561 ggatgccctt ctggatagca agctccaata atatcaaagt gaggggcttg ttccttgata
     7621 aactcaatca agtcggttgc atagcggaaa tccttttgtg gttctacgtc tggaataata
     7681 tccccacgaa gagccaagat tttctgaacc ccaactttgt ccaagtcagc aatagtttca
     7741 gcaaccttgt ccttagttag ataaatagct ggcaagtggg caatggtcgg aatcgccaag
     7801 tcattttgga taaagtcagc caaacgaacc gtcgtttcct tgatattaaa tttattattg
     7861 ctggcagtta cactgataaa atggggagcc aactcctgca tatcctgcaa ggctgaaata
     7921 atgttaccat tacccacggc tgggtttgga gggaacactt caaatgagag tgacggtgtt
     7981 tggcgtgaca tatgtaataa ccttttctag ttgatttctt tttgaacaac cactgtatgg
     8041 agagaaatcc aatcttacaa tttctcacgc gcagctttag ctgcttcaac aaggcggatc
     8101 aagctttctt ttgtttctgg gataccacgt gttttcaaac cacagtcagg gttgatccaa
     8161 actttcttgc ttggcacttt agcaaggatg gcttcgattg tgttgtcgat ttcgccttca
     8221 tttggtacac gaggtgagtg gatatcgtaa accccaggtc ccacttctgt ttggaagttt
     8281 ttcgctttga gttcgtccaa gatttcaagg tttgaacggc tggcttcaaa ggaaataacg
     8341 tctgcatcca tgttatcgat agctgggatg atatctgtaa attctgagta acacatgtga
     8401 gtgtggattt gtgtgtctga cgctactgtt gagtgtacca agcggaaggc aggaattgcc
     8461 cagtcaaggt aatcttcgta ccagtcacta cgacggagtg gcaatttttc acgaagagca
     8521 gcctcatcaa tttggatgat tttcacacca gcagcttcaa ggtcaagcac ttcatccttg
     8581 atagcaaggg cgatttgaag agtagaatcc ttgatagaga tgtcttcacg tgggaatgac
     8641 cagttaagga tggtaacagg tccagtcaac atacctttaa caggtttgtt tgtacgactt
     8701 tgtgcatagc tagaccattt aacagtgata gggttaagac gagtgacatc accccagatg
     8761 attggtggtt ttaccccacg cataccgtat gattgtaccc atccattttt agagaagagg
     8821 tatcctgaca agttttgacc gaagtactca accatgtcat tacgctcaaa ttcaccgtga
     8881 acaaggacat caaagtcaat atcttcttgc cacttgatcc attcgtcaat cgtttcagca
     8941 aggaaagcat cgtactcttt ttgagacaat tcacctttac ggtaagccaa acgtttggca
     9001 cgaacttctt ttgtttgagg gaatgaacca atcgttgttg ttggaagagc tggaagtttg
     9061 aaagcttctt cttggatagc ttcacgttct gcaaaggctg gcaaacgagt gtagtctgcg
     9121 tctgtcaagc cagcgatacg cgcacgaagt tcagcatttt caccaacacg ctcagtcgca
     9181 aagagttctt tgttggctgc aagagcttct gaaccttgac cattgcggat agcatccaag
     9241 tcacggattt catccaattt ttcaactgca aaggcaaagt ggttcaagag tgctggttca
     9301 aattcttcat tagcagttgt aaatggcaca tgaagaagtg agcaagagct tgtcaaaacg
     9361 atgttttcag ctggaatttg ctcaagaaca gccaagctct tttcgtagtt gttgcgccag
     9421 atgtttttac cattgacaat acctacatag agagtcttgt cagctgggaa gccaccttta
     9481 acgagttcaa gagttttctt accttcaaca aagtcaagac cgatagcatc tactggtaag
     9541 tttacaaggt cagcgtatac gtcacgaaca tcaccgaaat aagtttgaag caagacttca
     9601 agaccttttt tgtcagccaa gagtttgttg taaaggttca agaagagagc tttttcttca
     9661 gctgtcaagt cttttacaag agccgcttca tccaattgga tgcgagtcgc accaagttca
     9721 gccaatttag caaaaacttc ttggtaagca gccactaagc tatctacgaa gtcgtctgct
     9781 ttcacgcctt cttcaaagtc tgacaattga aggaaagtga agggacctac aagaacagga
     9841 cgagtgttca atccaagttc tttggcttct tggaactcat cgaaaatctt gtgaccagcc
     9901 aattttactt gagtgtcttt ttcaaattta ggaacgatgt agtggtagtt agtgttgaac
     9961 catttcttca ttggaagggc gcgaacgtcc cctttttctc cctggtaacc acgtcccaaa
    10021 gcgaagtagc gctcaaggtc agacaagtcc aagttttgaa cggatgcagg caccacgttg
    10081 aaaaggaaag ccgcatctag gaagttatca tagtgagaaa agtcatttga tggaatttca
    10141 gtgatgcctt tttctttgac aatgttccag tgtttagcac gcaagtcttt tgctgctgct
    10201 aaaagttctt cttctgagat ttcttttcta aagtattttt cagttgtaaa ttttaattca
    10261 cggaattcgc ccaaacgagg gaaaccgatg attgtagttg acatgatgtg tcctccaaaa
    10321 tttgttgttg aaactatctt aacagaaaag aaagcgtctg tataattgta aaaaattagg
    10381 gtttgatata gtttgaaact atatatctgt ttcggacaaa agaaaaagac ttgaagcaaa
    10441 cgtctcaaat cctttgtaat tcttacttta cagctatatt ccaattagaa tactaaaaca
    10501 tgttattagt aattcttata agtgactatg acctgttatt agaaaagact ataactgatt
    10561 ctagtcaact tttcccctgt tcaagtggaa cgactgctag tgtctttcct agactggtta
    10621 gaacttttaa gaccgtatcc aactgaagac tgctctctcc tgtttccatc ctagctatga
    10681 caggttgact cactccactg acttcttcca gctttttctg actgattcct tgttcatacc
    10741 tagcctcaat caactcgctc atgatagcca ctcgcatatc actttcaagg atttcctcct
    10801 tgctaaagag ctcagatgga catccttcca attactccca atagcactat tcttcatcac
    10861 ttaaccctct ttcttttacg tctatgtatt tttaaaagaa tgagcgaatt atgattcgat
    10921 agattgacca gcaggtttaa agttggtgct agcctgtttc ttaagcgctt ttccttttct
    10981 agggtaaagc agatcctgct tgcttaaccc caattttcca cgatgaatcc aatagtaaat
    11041 ggttgaaatt cccacgttaa cccctttagc catcaccatc atttca
//
LOCUS       DAVBBS010000062        10989 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVBBS010000062 DAVBBS010000000
VERSION     DAVBBS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10989
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.062"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            136..2316
                     /locus_tag="VQU32_001782"
     CDS             136..2316
                     /locus_tag="VQU32_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2473191.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDTSQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKNLTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            2368..3183
                     /locus_tag="VQU32_001783"
     CDS             2368..3183
                     /locus_tag="VQU32_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET2473192.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETLVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            3323..4666
                     /locus_tag="VQU32_001784"
     CDS             3323..4666
                     /locus_tag="VQU32_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2473193.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLEIVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            5043..6233
                     /locus_tag="VQU32_001785"
     CDS             5043..6233
                     /locus_tag="VQU32_001785"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET2473194.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            6786..7721
                     /locus_tag="VQU32_001786"
     CDS             6786..7721
                     /locus_tag="VQU32_001786"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HET2473195.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            7755..8792
                     /locus_tag="VQU32_001787"
     CDS             7755..8792
                     /locus_tag="VQU32_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET2473196.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNSSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            8965..9570
                     /locus_tag="VQU32_001788"
     CDS             8965..9570
                     /locus_tag="VQU32_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET2473197.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            9726..10256
                     /locus_tag="VQU32_001789"
     CDS             9726..10256
                     /locus_tag="VQU32_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HET2473198.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            10277..>10989
                     /gene="rlmN"
                     /locus_tag="VQU32_001790"
     CDS             10277..>10989
                     /gene="rlmN"
                     /locus_tag="VQU32_001790"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET2473199.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQGIVQESADGTVKYLFELPDGMLIETVLMCQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERISHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSISGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFP"
BASE COUNT         3341 a         2017 c         2395 g         3236 t
ORIGIN      
        1 tagaaatttt ttagaaaaaa gtgtaatttt tttataaaag cctttacaaa aaaaattaaa
       61 gtggtataat taaatcatag aaggtgcaaa cgttttcgta aaacgtttgc ctaaataaaa
      121 ataaaggaga tttgaatgat gaaagataca ttcaaaaatg tcttgtcttt cgaattttgg
      181 caaaaattcg gtaaggcttt gatggtagtt atcgcggtta tgccggctgc tggtttgatg
      241 atttcaatcg gtaagtctat cgtgatgatt aacccaacct ttacaccact tgtcatcaca
      301 ggtggaattc ttgagcaaat cggttggggg gttatcggta accttcacat tttgtttgcc
      361 ctagccattg gaggaagctg ggctaaagaa cgtgctggtg gtgctttcgc cgctggtctt
      421 gccttcatct tgattaaccg tatcactggt acaatttttg gtgtatcagg cgatatgttg
      481 aaaaatccag atgctatggt aactactttc tttggtggtt caatcaaagt tgctgattac
      541 tttatcagtg ttcttgaagc tccagccttg aacatggggg tattcgtagg gattatctca
      601 ggttttgtag gggcaactgc ttacaacaaa tactacaact tccgtaaact tcctgatgca
      661 ctttcattct tcaacgggaa acgtttcgta ccatttgtag ttattcttcg ttcagcaatc
      721 gctgcaattc tacttgctgc tttctggcca gtagttcaaa caggtatcaa taacttcggt
      781 atctggattg ccaactcaca agaaactgct ccaattcttg caccattctt gtatggtact
      841 ttggaacgtt tgctcttgcc atttggtctt caccacatgt tgactatccc aatgaactac
      901 acagctcttg gtggtactta tgacatttta actggtgcag ctaaaggtac tcaagtattc
      961 ggtcaagacc cactatggct tgcatgggta acagaccttg taaaccttaa aggtactgat
     1021 actagtcaat atcaacactt gttagataca gtacatccag ctcgtttcaa agttggacaa
     1081 atgatcggtt cattcggtat cttgatgggt gtgattgttg ctatctaccg taatgttgat
     1141 gctgacaaga aacataaata caaaggtatg atgattgcaa cagctcttgc aacattcttg
     1201 acaggggtta ctgaaccaat cgaatacatg ttcatgttca tcgcaacacc tatgtatctt
     1261 gtttactcac ttgttcaagg tgctgccttc gctatggctg acgtcgtaaa cctacgtatg
     1321 cactcattcg gttcaatcga gttcttgact cgtacaccta ttgcaatcag tgctggtatt
     1381 ggtatggata tcgttaactt cgtttgggta actgttctct ttgctgtaat catgtacttt
     1441 atcgcaaact tcatgattca aaaattcaac tacgcaactc cagggcgcaa cggaaactac
     1501 gaaactgctg aaggttcaga agaaaccagc agcgaagtga aagttgcagc aggctctcaa
     1561 gctgtaaaca ttatcaacct tcttggtgga cgtgtaaaca tcgttgatgt tgatgcatgt
     1621 atgactcgtc ttcgtgtaac tgttaaagat gcagataaag taggaaatgc agagcaatgg
     1681 aaagcagaag gagctatggg tcttgtgatg aaaggacaag gggttcaagc tatctacggt
     1741 ccaaaagctg acattttgaa atctgatatc caagatatcc ttgattcagg tgaaatcatt
     1801 cctgaaactc ttccaagcca aatgactgaa gcacaacaaa acactgttca cttcaaaaat
     1861 cttactgagg aagtttactc agtagcagac ggtcaagttg ttggtttgga acaagtaaag
     1921 gatccagtat ttgctcaaaa aatgatgggt gatggatttg cagtagaacc tgcaaatgga
     1981 aacattgtat ctccagtttc aggtactgtg tcaagcatct tcccaacaaa acatgctttt
     2041 ggtattgtga cggaagcagg tcttgaagta ttggttcaca ttggtttgga cacagtaagt
     2101 cttgaaggta aaccatttac agttcatgtt gctgaaggac aaaaagttgc agcaggagat
     2161 ctccttgtca cagctgactt ggatgctatc cgtgcagcag gacgtgaaac ttcaacagta
     2221 gttgtcttca caaatggtga tgcaattaaa tcagttaaat tagaaaaaac aggttctctt
     2281 gcagctaaaa cagcagttgc taaagtagaa ttgtaatata cttgaggttg gaagctgtat
     2341 tccaacctct tattttggga gaaaagaatg aaatttttaa cactcaatac tcacagttgg
     2401 atggagaaag aagcagagga aaaattccag attttgcttg aagatattct tgaaaaggac
     2461 tatgatttga tttgtttcca agaaatcaat caggagatga cctcgtcaga ggtggaggtt
     2521 aatgaccttt atcaagcttt gccagcagct gagcctattc accaagacca ttatgttaga
     2581 ctcttggttg aaaagttgtt agagcaaggg aaaaattact actggacctg ggcttataac
     2641 catatcggct atgaccgcta ccacgaaggt gtggctatct tgtctaaaac acctattgaa
     2701 gccagagaaa ttttggtttc agatgtggat gatccaacag actatcatac tcgccgtgtt
     2761 gccctagctg aaactttagt cgatggcaag gagctagcag ttgccagtgt tcatctctct
     2821 tggtgggata aaggtttcca agaagaatgg gcacgatttg aggctgtctt gaaaaaattg
     2881 aacaagccac ttttactagc tggagatttt aacaacccag caggtcagga aggttaccaa
     2941 gctattttag ctagtccatt aggcttacaa gacgcatttg aagttgctca agagaaaagt
     3001 ggtagctata ctgttccgcc tgaaattgat ggctggaaag ggaacactga accccttcga
     3061 atcgattatg tctttactac caaagagtta gcggtggaaa atttacatgt cgtatttgat
     3121 ggtaacaaga gtccacaagt gagtgatcac tatggcttga atgctatgtt aaactggaaa
     3181 taataactga aaagaggttg gaactataaa attccagcct tttcttacta gagaagctac
     3241 tggaaatagc ctaaataagt gagactactg taatggaata aaatatggta taattgataa
     3301 gatagataga atcgaggatg ttatgtcatt tacgaaattt caatttaaaa actatattag
     3361 agaagccttg aaggagttaa aatttacaac tccaacagag gtgcaagata agttgattcc
     3421 tattgttttg gcaggtcgtg acctagtagg agaatcaaaa acaggttcag gtaagactca
     3481 tactttcttg ttaccgattt tccagcaatt agatgaagct agcgatagtg tacaagcagt
     3541 gattactgca ccgagtcgtg agttggctac tcaaatttac caagtagcgc gtcagatttc
     3601 agctcactca gatgtcgaag ttcgtgtggt taattatgtg ggtggtacgg ataaggctcg
     3661 ccagattgag aaattggcaa gcaatcagcc tcatattgtt attggaacac caggccgtat
     3721 ctacgacttg gttaaatctg gtgatttagc tattcataaa gccaagacat ttgttgttga
     3781 tgaagcagat atgaccttgg atatgggatt cttggaaatt gttgataaga ttgctggcag
     3841 tcttccaaaa gacttgcaat tcatggtctt ctcagcgact atcccacaaa aactgcaacc
     3901 attcttgaaa aaatacttat caaatcctgt tatggagaaa attaagacta aaacggttat
     3961 ttctgacacc attgataatt ggttgatttc gaccaaggga catgataaga atgctcaaat
     4021 ttaccaattg actcagttga tgcagccgta tttggcaatg atttttgtta acactaaaac
     4081 gcgtgctgat gaattgcatt catatctgac tgctcaaggc ttgaaggttg caaaaatcca
     4141 tggagatatt gcccctcgtg aacgcaagcg aatcatgaat caggtgcaaa atctggattt
     4201 tgagtatatt gtcgcaacag atttagcagc gcgtgggatt gacattgaag gtgtcagcca
     4261 tgtcatcaat gatgccattc cgcaagactt atcttttttt gttcaccgtg ttggtcgtac
     4321 tggacgaaat ggcctaccag gtacagctat taccctttat cagccaagtg atgactcgga
     4381 tatccgtgag ttggagaaat tgggaatcaa gtttagtcct aagatggtca aagatgggga
     4441 atttcaagat acctatgacc gtgatcgtcg tgccaaccgt gagaaaaaac aagataaact
     4501 tgatatcgaa atgattggtt tggttaaaaa gaaaaagaaa aaagtcaaac cgggttataa
     4561 gaagaaaatt caatgggcgg ttgatgaaaa gcgccgtaaa actaagcgtg ctgaaaatcg
     4621 cgctcgcggt cgtgcagagc gtaaagctaa acgccaaaca ttttaataga aattgttgga
     4681 gtactgagct ccaacttttt tatttatgag aacgaactat ctaaaccgaa acactacatt
     4741 aaagactgca aattgcgatt aaaaatggta taatgataaa gttatatagt cccgataaga
     4801 tggtaggtat ttattacgaa gagttttcct atcagtagtt tgcaattcgc aaagaatgac
     4861 ggcttcgagg tcgctcgtct ttctacggtt gccgccatac tgcggtcacc ctatgcgcct
     4921 tactagcttc agaaataaaa aagtatcgtt tttatttctg aagctagtaa ctcttagata
     4981 atggaaattc ctatcagtac tttgtaactc tataacaata ttttttaagg ggggacattt
     5041 ttatgtcaga acgtaaatta ttcacgtctg aatctgtatc tgaggggcat ccggataaga
     5101 ttgcagacca aatttcagat gcgattttgg atgctatttt agcaaaggat ccagaggcgc
     5161 acgttgctgc tgaaacagct gtatatactg gttctgtcca cgtttttggt gaaatttcta
     5221 caaatgccta tgtggatatt aaccgtgtgg ttcgtgatac cattgcagag attggttata
     5281 ccaatacaga atatggattt tctgctgaga cggtgggagt acacccatct ttggtggaac
     5341 aatctcctga catcgctcaa ggtgttaacg aagccttgga ggttcgtgga aatgctgatc
     5401 aagatccact ggacttgatt ggagcaggtg accaagggct catgtttgga tttgcagtag
     5461 atgaaacaga agagctcatg ccattgccaa ttgcactcag tcataaattg gttcgtcgtc
     5521 tggcagaact tcgtaagtct ggagaaatta gctatctccg tccagatgca aaatcacaag
     5581 ttacagttga gtacgatgaa aatgaccgtc cggtacgtgt agatacagtc gttatttcta
     5641 ctcagcatga tccagaggcc actaatgaac aaatccatca agatgtgatt gacaaggtca
     5701 tcaaagaagt tattccatct tcttatcttg atgataagac aaaattcttt atcaatccga
     5761 caggtcgttt tgtaatcggt ggtcctcaag gggactcagg tttgactggt cgtaagatta
     5821 ttgtagatac ttatggtggc tactctcgtc atggtggtgg tgccttctct ggtaaagatg
     5881 cgactaaggt ggatcgttca gcctcttatg cggctcgcta tattgccaag aatatcgttg
     5941 cagcaggcct tgctaagaag gcagaagtgc agttggccta tgctatcggt gttgcgcaac
     6001 ctgtttctgt tcgtatcgat actttcggta caggaacagt agctgaaagt caacttgaaa
     6061 aagcggctcg tcaaatcttt gaccttcgcc ctgcagggat tatccaaatg ctggacctca
     6121 agcgtccaat ttaccgtcaa acatcggctt acggtcacat gggacgtaca gatattgatc
     6181 ttccatggga acgtttggat aaggtagatg ctttgaaaga agcagtaaaa taagatttta
     6241 agaggggaac gtcctctctt ttttatagtt tttaactata ctgggatact gttctgaaaa
     6301 tccattttgc gaaagtagag atttacatgt ataatggatt gaaactagaa tagtacacct
     6361 caacttctaa aacattgtta gaaatcgatt tgactgtcct gatcgatttc tcctgttctt
     6421 gtttcatttt actatatttc tttaaaaatg ataaaggttg agatttctcc tcgtaataga
     6481 taatcatggg gatatttcaa tccaaagttt tattcgttat cacttgacta ttgcaaggtt
     6541 ttctagagca acagagtcat ggaatggact catggttgag atttctcctt gttgcttgga
     6601 cttcattcaa aagtctgtta cccaagcctt gttcaaactt ccaatacact agctgtttcc
     6661 atagcatgac ttctgtacta gactttcttt tccgaataaa tagatagaac cacagaatct
     6721 agtaaaccta gaattaaaat tatggtataa tattagcaat aaaagaaatc tggaggatta
     6781 gaatcatggt atcaacgaaa acacaaattg ctggttttga gtttgacaat tgcttgatga
     6841 atgcagcagg tgtggcttgt atgacgatag aggagttaga agaggtcaaa aactcagcgg
     6901 caggaacctt tgttactaag acagcgacct tggacttccg tcaggggaat cctgagccac
     6961 gctaccaaga tgttccactt ggttccatca actctatggg cttgccaaat aatggcttag
     7021 actattattt ggattatctt ttagatttgc aggaaaaaga gtcgaaccga actttcttct
     7081 tatctctggt cggcatgtct ccagaggaaa cccatactat tttgaaaaaa gtccaagaga
     7141 gtgattttcg tggtctgact gagctaaatc tttcctgtcc aaatgttcca ggtaaacctc
     7201 agattgccta tgattttgag acaacagacc ggattttggc agaagtgttt gcttacttca
     7261 ccaaacctct tggaattaaa ttgccacctt attttgatat tgttcacttt gaccaagcgg
     7321 cagctatttt caacaaatat ccgctcaagt ttgtcaactg tgttaactct atcggaaacg
     7381 gcctctatat agaagacgaa tctgtcgtta ttcggcctaa gaatggtttt ggtggaattg
     7441 gtggagaata catcaaaccg actgctctag ccaatgttca cgccttttat caacgtttaa
     7501 atcctcaaat ccaaattatc ggaacaggtg gcgttctgac tggtcgagat gcctttgaac
     7561 acatcctctg tggagcaagt atggtgcagg tgggaacgac ccttcacaaa gaaggcgtca
     7621 gtgcttttga ccgcattacc aatgaactga aagcaatcat ggtggaaaaa ggctacgaga
     7681 gcttagaaga tttccgtggg aaattgcgct atattgacta aattaaatcg aaaaatctga
     7741 agaaaggaga gacgatgcta gccattgaag aaagtcagaa gttgacttta tcaaatttac
     7801 cgagcctgag cctatttaca gggacagatc agggtcagtt tgaagtgatg aagagtcaag
     7861 tgttgaaaca gattgggtat gattctgctg acctcaactt tgcctacttt gatatgaaag
     7921 aagtagttta caaggatgtg gaactggagt tggtcagcct tcctttcttt gcggatgaga
     7981 aaatcgtgat attagattat tttatggata tcacgactgc taagaaacgc tttttgacag
     8041 atgatgagct taagtcattt gaggaatacc ttgacaattc ttctccaaca accaagttga
     8101 taatctttgc agaaggaaag ctggatagca aaagacggtt agtcaaatta cttaagcgtg
     8161 atgccaaggt cttcgatgca gtagaagtaa aagaacaaga attgcgccag tatttccaaa
     8221 agtggagtca gaaacaaggt ctgcagttta ccaatcattc ttttgaaaat ctcctcatca
     8281 agtcggggtt tcaatttagc gaaatccaga aaaatcttct ctttttacag tcctataagg
     8341 cgaattctgt tattgaggaa gaggatattg ttaacgcaat tcccaagacc ttgcaggaca
     8401 atatttttga tttaactcag tttattctga ctaaaaagat ggatcaggcg cgcgatttgg
     8461 tgagagactt gaccttgcaa ggggaagatg aaatcaaact gattgcagtc atgctgggac
     8521 aatttcggac ttttactcag gtgaagattt tggcggagtc tggccaaaca gaatcgcaga
     8581 ttgcaagtag tttaggtagt tatctgggac gtaacccaaa tccttatcaa atcaagtttg
     8641 cattaagaga ttcgagagga ctttctttga gctttctgaa gcaagctatt tcctatttga
     8701 ttgagacaga ctatcagatt aagacaggtc tttatgaaaa aggtttcctt tttgaaaagg
     8761 cactcttaca gattgctagt caggtcaatt gacatttgtt gaaactacaa cccgcgtcag
     8821 gattatcttg tcgtgggttt cttgctgatt tgcttatttt attagaaagt atgtcagtga
     8881 tttccttatc tataagcaaa aaacttgaaa aaagtgaatg tttgcgttat cattttcata
     8941 tagaaagaaa aagaggtatt acagatggct attatcttac cagaacttcc atatgcatac
     9001 gacgctttgg aaccatacat cgatgcggaa acaatgcatt tgcaccatga caaacaccat
     9061 caaacttatg tcaacaatgc caatgcagct ttagaaaaac accctgaaat cggtgaagac
     9121 cttgaagcct tgcttgctga tgtagaatct atcccagctg atatccgtca agcacttatc
     9181 aacaatggtg gcggacactt gaaccacgct cttttctggg aattgatgac tcccgagaaa
     9241 acagctcctt cagcagaact ggcagcagca atcgatgcaa catttggttc atttgaagaa
     9301 ttccaagcag ccttcactgc agcagcaaca actcgttttg gttcaggttg ggcatggttg
     9361 gttgtcaaca aagaagggaa acttgaagtg acttcaacag caaaccaaga cacaccaatc
     9421 tcagaaggta aaaaaccaat cttgggcttg gacgtttggg aacatgctta ctacgtgaaa
     9481 taccgcaacg tgcgtcctga ctacatcaaa gctttctttt cagtaatcaa ctggaataaa
     9541 gtagatgaat tgtacgcagc tgctaaataa tgatagttgg agggaagaat tgttcttctc
     9601 tttttaggtt ataagattct ggtctgacaa aatcgtcaga cttttttcat ttttatgaga
     9661 aacgtgctaa ctgctagaaa ttatggtaga ataaagtatt aagtaatcgt ggaaaaagga
     9721 tatctatgcg aaaagaaatt gcacctgaat tatacaacta taacaagttt cctggtccgg
     9781 agttccattt acacggggat aaggtcgaaa cggaagggat agctttttcc ttggttgaaa
     9841 atatcaagga tgcctttgat gtgacgactt ttaatcagcg tttttcagaa gtcttaacca
     9901 agtttgatta tatcgtgggg gactggagca acgaacagct tcgcctacga ggtttttaca
     9961 aggatgaccg aacagaagaa aaacttgaaa aaatcagtcg tttacaagac taccttttag
    10021 agtattgtag ttatggttgt gcctattttg tcttagaaaa tgaagcccct aagcgagcat
    10081 catttgacaa gaaaatgcgt aagacggaag aagaaacacc ttctagaaaa ggaaagaaac
    10141 cgactcaaac taaacgaaag tcgaatgcag ataagaaaaa tagacgtcgt cagaaagacc
    10201 agcattctca gaaagaggac aaggaacaac gtcattttgt cattcgtcag aagtgagtag
    10261 agaataagga gaagaaatga aaccctcaat tcatagtttg gcacatcaaa ccatgcagga
    10321 atgggtattg gagcagggag aaaagaaatt ccgtgcagat caaatctggg aatggctcta
    10381 ccgtaaacgt gtgcagtcat ttgaagaaat gaccaacctt tccaaggatt tgattgctaa
    10441 gctcaatgac cagtttgtgg tcaatccctt gaaacaaggt attgtgcaag agtctgctga
    10501 tggtacagtc aaatatctct ttgaattgcc tgacggtatg ttaattgaga ctgtactcat
    10561 gtgtcaacac tatggtttat ctgtctgtgt gaccactcag gtcggctgta atatcggttg
    10621 taccttctgt gcctctggtt tgattaagaa gcagcgtgac ctcaataacg gtgaaatcgt
    10681 agcgcaaatc atgctggttc agaaatactt tgatgagcgt ggtcaggatg aacgcatcag
    10741 ccatatcgtt gtcatgggaa tcggtgagcc ctttgataat tacaacaatg tcttgaattt
    10801 ctttcgtact atcaatgatg ataaaggaat ggctatcggt gctcgtcaca tcacggtttc
    10861 aatctcaggt ttggctcata aaattcgtga ctttgctgat gaaggggttc aggtcaatct
    10921 tgccgtgtct cttcacgcac ccaacaatga acttcgttca agtatcatga agattaaccg
    10981 tgcctttcc
//
LOCUS       DAVBBS010000063        10834 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVBBS010000063 DAVBBS010000000
VERSION     DAVBBS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10834
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.063"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            1..269
                     /locus_tag="VQU32_001791"
                     /pseudo
     CDS             1..269
                     /locus_tag="VQU32_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(385..1188)
                     /locus_tag="VQU32_001792"
     CDS             complement(385..1188)
                     /locus_tag="VQU32_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HET2473200.1"
                     /translation="MQSTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRSEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(1467..2222)
                     /locus_tag="VQU32_001793"
     CDS             complement(1467..2222)
                     /locus_tag="VQU32_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HET2473201.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(2292..4943)
                     /locus_tag="VQU32_001794"
     CDS             complement(2292..4943)
                     /locus_tag="VQU32_001794"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET2473202.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYSTTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPKFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTDDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNEAGEIYVGEEAPAGDGWKQDEDVLDTWFSS
                     ALWPFSTMGWPNTETTDFKRYYPNSTLVTGYDIIPFWVSRMIFQGIEFTGKSPFKNAL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIDKYGTDSLRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAEYPTVNPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLVDLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(4936..5514)
                     /locus_tag="VQU32_001795"
     CDS             complement(4936..5514)
                     /locus_tag="VQU32_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET2473203.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYQLMYLKQIQNLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKHKPLKRHIEVSEREILETAETLQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(5514..5984)
                     /locus_tag="VQU32_001796"
     CDS             complement(5514..5984)
                     /locus_tag="VQU32_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2473204.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGYG
                     IVVKGTDKIVGSVDFNHRHEDDVLEIGYTLHPDYWGRGYVPEAARALIDLAFKDLGLH
                     KIELTCFGYNLQSQRVAEKLGFTLEARIRDRKDAQGNCCDDLRYALLKSEWEVI"
     gene            complement(5971..6549)
                     /locus_tag="VQU32_001797"
     CDS             complement(5971..6549)
                     /locus_tag="VQU32_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET2473205.1"
                     /translation="MKQSFKTNKLYYGFPVFILGYQDQNFGYNITTCSSSYSLGDWVV
                     IGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEKLERLGVHYEISERTQ
                     TPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDEKGHFKNQLFAPTYFM
                     GDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            complement(6832..7227)
                     /locus_tag="VQU32_001798"
     CDS             complement(6832..7227)
                     /locus_tag="VQU32_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HET2473206.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(7323..7577)
                     /locus_tag="VQU32_001799"
     CDS             complement(7323..7577)
                     /locus_tag="VQU32_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HET2473207.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            complement(7586..8920)
                     /locus_tag="VQU32_001800"
     CDS             complement(7586..8920)
                     /locus_tag="VQU32_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HET2473208.1"
                     /translation="MTDEWIHILRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVLDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYELMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTVKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKRLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(8920..9153)
                     /locus_tag="VQU32_001801"
     CDS             complement(8920..9153)
                     /locus_tag="VQU32_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HET2473209.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLTGTPMDKIVRTLEANGYEVIGLD"
     gene            9184..9258
                     /locus_tag="VQU32_001802"
                     /pseudo
     CDS             9184..9258
                     /locus_tag="VQU32_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            9247..9372
                     /locus_tag="VQU32_001803"
     CDS             9247..9372
                     /locus_tag="VQU32_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl-tRNA synthetase"
                     /protein_id="HET2473210.1"
                     /translation="MDRTDEVSNHTYSKVKLTWFEEIFEEYKMILLLILYHMERD"
     gene            complement(9422..9562)
                     /locus_tag="VQU32_001804"
     CDS             complement(9422..9562)
                     /locus_tag="VQU32_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473211.1"
                     /translation="MKFFWTILAILFIKPIIGIVKFFWMIISFAVQLLFYKILDWFFK
                     LI"
     gene            complement(9559..9741)
                     /locus_tag="VQU32_001805"
     CDS             complement(9559..9741)
                     /locus_tag="VQU32_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473212.1"
                     /translation="MNPIKAFAKIYGNYFLTVQGVKVMKTIKKDDHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(9743..10526)
                     /locus_tag="VQU32_001806"
                     /pseudo
     CDS             complement(9743..10526)
                     /locus_tag="VQU32_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3154 a         2389 c         2005 g         3286 t
ORIGIN      
        1 cgacacgatt cttatcatcg gttcctaaga gtaagggaat tcaagcatct atgtcacgca
       61 agggaaacag ccaagacaac ggtatgatgg agtccttctt tgggattctg aaatcggaaa
      121 tgttttacgg ttatgagaag tcgtttcagt cgcttaagca attggaacaa gccattatag
      181 actatattga ttactacaac aataaacgaa ttaaggtaaa actaaaagga cttagccttg
      241 tgcaatacag aactaaatcc ttcggataaa ttatttgtct aactttttgg ggtcagtaca
      301 cctaaaactt tgatgatata cgttttcttg tgagaatatt tacttcattt ttgcctaaaa
      361 ttcaatgttt actcagtatt tggattatga aaaatcgagg tctaaatcta gatacatttt
      421 ttctgaagac aaatcatttt gaccaccgag caagagattt tcaaaaaaag ctgttaaaaa
      481 ctcagaacgt cgctgtaaaa tctttgcatt atctaatacc aaggcatcac gaaaatattt
      541 ggaatgttgc tgaaatggtg tattatcaat atcaaaacca aactcacgaa gatactgaat
      601 caaaaagacc gttactgtcc gagtgtttcc ttcgcgaaat ggatgaatct gccagattcc
      661 tgaaataaaa tgctggattt gtttaaccac atccgcctga gttagtgtcg catatgcaac
      721 ttgtttttcc tgattaaaat cataatctaa ggtcatttga atcatggagt aatcagagta
      781 cacaacactt tcaccattca aaacaggttc attctttgtg atattggtct gacgaaattg
      841 ccccaccgga atagagggtt caaatatatc ttgaaacaac tccttatgaa tagcaagtaa
      901 ggtcgcagga ctaaagctaa agcctcttcg agacaatagt tctacaatac gtagagaaac
      961 caagtctgcc tcttccgtac ttgcatcaat agtatggtga taagccgttg catcctcata
     1021 aacctgctca taagtcagtt ctccccggga ctgtttctca gccaaagatt ccatatacgc
     1081 tgatggcact agattgtcaa ctttctgcag accaaaacct atccgccata aatcacgctt
     1141 cgcttcataa gacaagtttg gattgtcaat gttgtaagtt gattgcatag aaatatcctt
     1201 tcaattgttt ttcttcatta ccagtaaatc acgaaattgg ttggaaactg gcttaaacta
     1261 taaaaagtcc tcacaatagg ttctatgata tatatatata tacttcatgt aaataataaa
     1321 agagttataa tcagcactac tgataaattc aataccgatt tcgccatctt aaaacttagt
     1381 caaacgaata ccgttgattt ccagttcatt aagtcgttga cctgtcaaga cttctcgttc
     1441 cttcaactgg aaaagttaag ggggaactaa tacaaatcct ctacgcttct atattcaatg
     1501 atttcatttt tgataatatt ctttttaaac tcaaaatgtt ttaaaggatt atcgattaac
     1561 actaactttg gaatatcatc tagatttgtg accaaggata aaataaaatc cgattgaaac
     1621 tccttgactt tttcaaattc ttttgcagtt aaacgcacac gccccttaga ttttcggata
     1681 ccttttactt cggcaaggta agaatactct tggacatcta cctgaaagtc atatccatca
     1741 ccataaagac gggcatcggt taactgtcca ccttggaatt tttcttcttt atcaaaatga
     1801 aggataaaat aattctcagc ctccattccc gtttcctgta gacgtttgaa tttagttcga
     1861 actattggtt tttcaacgat ggttatgcct gttttcttcc cttcactggc aagtaacatt
     1921 ttaactattt cagcataact atgaacatct tcatttccaa acatcatatc aattaaatct
     1981 ttacgaaaac gataaacttc agctttctgc caccaacctt ttcgattatt gtcaaaataa
     2041 ggatcaaata aatccattct attttttaca actccagttg tttcagcaat tcccagagaa
     2101 accacatagc gataaaactc cgacttgcta gagcattgaa attcttttat aaaaagcttg
     2161 tcaaacttgg caagaccgta gccaattaaa tttaacaatt cataatgagg gtgtttggac
     2221 atcactttct ccatcaatag atattgattt cattatacca aaaaacacgg ctcatcaccg
     2281 tgtttctgtg tttatttcac caacttcttc atctcatcaa tacgtgctac ggtcgcatca
     2341 tacttagctt ggtaatctgc ttgcttgtcg cgttcttttt ggacaacttc tggtttagcg
     2401 ttggcgacga agcgttcgtt gctgagtttt ttgccaacca tgtcgagttc tttctgccat
     2461 ttggcaagtt ctttttccag acgtgccaat tcttcttcga cattcaagag gtctaccagt
     2521 ggcaagaaga tttcggctcc tgtgatgacg cttgacatag ccagttcagg tgcagggatg
     2581 gttgatgcga tttccaagtg ttcaggattt gtgaaacgtt tgatgtagtt gacattgctg
     2641 ttaaagaagg cttccaagtc gctatcgctt gtcttaacaa ggatagtgat aggcttgctt
     2701 ggagcaacgt ttacttccgc acgcgcatta cgaacggcac ggatcaagtc tttaaggctt
     2761 tccacaccag tgtgagcagc aaggtcttca aaggctgggt tgacagttgg gtattctgct
     2821 gtaacgatag atccttctga gatttgtcca aagatttcct ctgtcacaaa tggcatgatt
     2881 gggtgaagga gacgaaggat cttgtccaaa gtgtatagta gaacagaacg tgtgatgact
     2941 ttctcttctt cgttatcgct ataaaggact tccttggtca actcaacata ccagttggca
     3001 aattcttccc agatgaagtt gtaaaggata tgaccagcca caccaaactc gaacttatca
     3061 aagttttcag taacttttgc aatggtttcg ttgagattgt ggagaatcca gcggtccgtc
     3121 acattaccag cctcacctgt tgcaactttt gtgacattgt catgcgccac atccagcgtc
     3181 aaaccttcat tgttcatgag gatatagcga gaaatgttcc aaattttgtt aataaagttc
     3241 catgaagcat ccattttctc gtaagagaaa cgaacgtctt gaccaggagc tgagccgttt
     3301 gacaagaacc aacgaagact atctgtacca tatttatcaa taacatccat tgggtcaatg
     3361 ccatttccaa gagatttaga catcttacgt ccttcttcat cacgaattag accatggata
     3421 agagcatttt taaatgggct ctttcctgta aattcaattc cttggaaaat catgcgtgat
     3481 acccagaaag gaataatatc atagcctgtt acgagtgttg aatttggata gtagcgttta
     3541 aagtcagtag tttcggtatt aggccagccc atggttgaga agggccagag ggcagagctg
     3601 aaccaagtgt caaggacatc ttcgtcctgt ttccagccat cgcccgctgg ggcttcctcg
     3661 ccgacataga tttcgcctgc ttcattatac caagcaggga tttggtgacc ccaccagagc
     3721 tgacgggaaa tcacccagtc gtggacattt tccatccatt gcaggaaggt gtcgttgaag
     3781 cgcggtgggt aaaattccac cttgtcatct gtgtcttggt tggcaatggc gttcttggcc
     3841 aattggtcca tcttgacgaa ccattgagta gacaagcgtg gctcaactac gacacctgtc
     3901 cgttctgagt gaccgactga atggacacgt ttttcgattt tgacaagggc accgatttct
     3961 tccaacttag caacgactgc cttacgagct tcaaaacgat ccatgcctga aaattcaaag
     4021 gcaagctcgt tcatagttcc gtcgtcgttc atgacgttga cttgtggcaa gttatgacgt
     4081 tggccaacca agaagtcatt tggatcgtgg gcaggtgtga ttttcacgac accagtacca
     4141 aacttaggat ctgcgtgctc atcaccaacg attgggatta atttattagc aattggaagg
     4201 atgacatttt taccaatcaa gtccttgtag cgcgggtctt ctgggttgac cgcaaccgca
     4261 acgtccccaa acatggtctc aggacgagtt gtagcaactt caagggcgcg tgaaccatct
     4321 tccagcatgt aattcatgtg atagaaggca ccttcgacgt ccttgtgaat aacctcgata
     4381 tcagaaaggg ctgtgcgagc tgctgggtcc cagttgatga taaactcacc acggtagatc
     4441 cagcctttct tgtaaaggtc cacaaagacc ttacgaacag cttttgacaa accttcgtca
     4501 agagtgaaac gctcacgaga gtagtctaca gagagcccca tcttgcccca ttgttccttg
     4561 atagtagtgg aatattcgtc tttccattcc cagactttcg tcaagaaaga ctcacgacca
     4621 aggtcatagc gggaaatgcc ctcaccacgc aagcgctcct ctaccttagc ctgagtggca
     4681 atccctgcgt ggtccatccc aggaagccaa agggtatcaa aaccttgcat gcgtttttga
     4741 cggatgataa tatcttgcaa agttgtatcc caagcgtgac caaggtgaag tttacctgta
     4801 acgtttggtg gtggaataac gattgaataa ggcttagcct tttgatcgcc tgaaggcttg
     4861 aaaacatcag catcaagcca tttttggtaa cgaccagcct caacctcggc tggattgtat
     4921 ttagatgaaa gttctttaga catgtgtgtg tcctttctct attttgttta ttttattttg
     4981 aatttgctta gcagcttctt ctgcagacaa attcgtatta tttattttaa agtagtggtg
     5041 caactcattc ggttgatatt gggaatttaa ttgaagtgtt tcagcggtct ctaaaatttc
     5101 tctttcagat acctcaatat gtcgttttaa gggtttgtgc tttaatcgat tctccgttcg
     5161 atttcgacgt atgcactctt caagacttgt ttccaactca acaaaaagaa tctcttgatg
     5221 ataatcatcc aacaaattct gaatttgctt taaatacatc agttgatact gatttgaaaa
     5281 atcaattaca tctgttaaaa ttactgatcg ctgatttctt gcacttgctc caaggaatga
     5341 aaaagtgatt ccacgaacaa attcccacat ttcctctgta tagtcctgat agatttctag
     5401 tgcaaaatca atggcttgat ggttataaaa tagggtagca tccgtcagtc gagataattc
     5461 ttgaccaatg gtcatttttc ctgatgctgg agcaccaatg atgaaaagat gcatcaaatc
     5521 acctcccact cactcttcag caaggcatat ctcaaatcat cacagcagtt gccttgagca
     5581 tctttacggt ctcttatgcg agcttcgagg gtaaagccaa gcttttccgc gactcgttga
     5641 ctttgaaggt tatatccaaa gcaagttagt tcaatcttgt gaagacccaa atctttaaag
     5701 gctaagtcaa tcaaggcacg cgcagcttct ggcacataac ctcgccccca atagtctggg
     5761 tgtaaggtat agccgatttc cagcacatca tcttcatggc gatggttgaa atcgacagag
     5821 ccaacgatct tatccgttcc tttgacgaca atcccgtagc ctgctggata ggcgacctct
     5881 ggcaaactag catagtcaaa gatatcctca gcatccgcca cagtacggac tcgtaaaacg
     5941 agacgttctg tttcgatttt atctggcagc tcagttcttg ccatccttct tcctcgcttt
     6001 tttgatgaaa ctgcccttca tatctacacg cttatccaga tagcgataaa cgcgctgata
     6061 tccatctccc atgaaatagg ttggggcaaa cagttgattt ttaaaatgtc ccttttcatc
     6121 caggagttct ggggcaacaa gtcgctcaag aatcttggca aagatgtggc aaataccgtc
     6181 ttcctcaaca atcctatcta cccgacaatc taaaacaagt ggacaggcgt ctaaaatagg
     6241 agtctgagtt cgttcagaaa tttcataatg cactcccaaa cgttccaatt tctcctgatg
     6301 actgataaaa ccagcctgct ccatcgcaag catagaagtt tcatcagaaa tattcacagt
     6361 aaatttttga tactgtttga tctgctctgc ggcattctct ctcgcaacga ctccaatcac
     6421 aacccaatct cctagactat acgaggaact acaggtcgtg atgttatagc caaaattctg
     6481 atcttgatat cctaaaataa aaacaggaaa accataatat agtttattag ttttaaatga
     6541 ttgttttata acaacctcct ttgactaaga cacaaaagaa aagccctgcc atctacatga
     6601 cagggacgaa tgtatttatc cgcggtacca cccaatttcg ggccaagccc gcatctttag
     6661 tctttaaaat aaaaagacag aaatatttca ttttttcagt cattagcaac cagttttgac
     6721 acatttgtgg acttctcagc gccgccactt tctgtaaaat gtgggattca aaacacctct
     6781 aatagctcta ttgtagcaag tttttctcgg aaatgcaagg ctcaagaaaa attacttgaa
     6841 cttgatgcca ttttctttca atttcttgat ggtagctggg ccgattcctt tcaaggcaag
     6901 gagttctttt tcagtccagt ttttgaaatc tgaagcagac ttgattcctt catcatagaa
     6961 agtcttggca cgatcaagtg gaagtccacc caagtttgct gcaaaatctt ctacagaatt
     7021 agctactttt tgagcttgct ctttggtagc ttctactgct tgttcaactt ttttagaaac
     7081 agccttacca gctgcgcttg ctgactgttc tgcacgcttc acgacttttt ttgtctcatc
     7141 tacaacctta gtcacagtac ttgaaaaggc acctgcgcga cggaggccat ttcgtaattg
     7201 ttttttacga ttgagtttct ttgacatgat aaaatccttt caataaaaaa tccctgctct
     7261 gacaaggatt taacataaac attctatcaa gattttagtg aaaaatcaag aatctttctc
     7321 gtttaataat ttcgctcacc aaacaagcct tctggcaaat catggaatcc cttgcctact
     7381 ttgaagtttt caaacttacc aagcaagaac tgataagcat ccaagcgact ctcgtagcga
     7441 atcatggcat ctttggcacg ctcgtcctgg tcttcttcgt aaactttttg actttccttg
     7501 tgcgccatgt cgttaatcat gatttgggta ttgacccaag cttcaaattc cttcataaat
     7561 tcttgttcgt aactcatttt tctccttatt ctaatccacg aaaatagtcc gtatcaatct
     7621 cacggtaggg ctggatatcc tgaacatact ccaacactcc ttggaattct ccgtcttcat
     7681 cgtgtactgc agcataggtg atgtggacaa acttacctcg cgactcagac ttgaaccaca
     7741 tttcatactt gtctttgctt ccctcacgaa gcctcttcat gatagttttg accttgtcca
     7801 agtacttagg cggatggcag agttcgacat tgcgcccgac ttgagacggc gtccgtttga
     7861 aaatcatctc atcagctggc gtattgtcat tgtaatactg gaaaatatct tctttattga
     7921 caaaggtaat ctccataggg agatgattga ggatgagatt ggcctgctcg actgaaagat
     7981 agccattacc aaaagcctgt tgactatggc ggtccagcac agcttccttt tccttagggg
     8041 taaaggtaat ggtaaaatgg ccttctggcg tatctatgac ttgttgaact tgaccttctg
     8101 ccgtatctag ctgtacaggc tcctctgcaa tcttttcctc aataaagctc tgtcgttctg
     8161 gcacccattt ctctgacgga cggatgatgg catagccata ggcatcgctc tcctccgcaa
     8221 tctgaagcca gtcatcctga gtaaaagact caaggagaat catgaggagg atggactctt
     8281 ccttgaaaat catactttca aactctgtcg caaaagcttc aaaagcttcc tttacactgc
     8341 taattgacac ttctggtagt gacttgactg tcgttagagc tgtttgaaag agttccctaa
     8401 tctgatcatc cactccccac ataactttgg gaggtgaatc gtgtccatag cgctccatga
     8461 taggaaagaa gagttcttcc ttacgttggt aatggatgtc aaattgcccc acaagcccca
     8521 tctgacgcac caagcccttg cgcatctccg caagcatttc ctcgtcttcc atcaactcat
     8581 aggtatctaa caatctacga atgcgaatca aagctgcacg gagggccaaa ttttcttcct
     8641 taaagacacg aactgggtgc ccagggtgtt cggtatctaa aacttcgaca cctttgatag
     8701 catttttaaa aagattggca tggacatcac agagttccat aacatcttca aaagtgacgc
     8761 ccgaatccga gttcatcagc tcgtgctcca taagggaaat ctcgatggct gacacgcccg
     8821 taaaggtcgc atcaaagcga tcttgaaccg actcaggaga ggcgccattg tgcaattcta
     8881 acaaaatatc ccgtaggata tgaatccatt catctgtcat tagtctaatc caatcacttc
     8941 gtagccattc gcttccagtg tgcgtacaat cttgtccata ggagttcctg ttagcttaga
     9001 accctgttta agtgatactt tacgaccaac tgtattgcgc attaagggat tggcaagggg
     9061 tttaaaaccc aactccacta gaatttccaa gacttctgga tgcttgtcca ccacttctgc
     9121 aacaggaatt gacacatcga tgatattgtc catgacgacc tccattgtat gttctaatac
     9181 tcaatgaaaa tcaaagagca aactaggaag ctagccgcag gttgctcaaa acagtgtttt
     9241 gaggttgtgg atagaactga cgaagtcagt aaccatacct acagcaaggt gaagctgacg
     9301 tggtttgaag agattttcga agagtataaa atgattttac tgcttatatt ataccatatg
     9361 gagagagatt aaaaaactag cagtggagtt cctgctagtt ttctgcgact tggattacca
     9421 tctagataag tttaaagaac caatccaata tcttgtaaaa cagcaattga actgcaaaag
     9481 aaatgatcat ccaaaagaat ttcacaatcc caataatcgg tttgataaaa agaatagcaa
     9541 gaattgtcca gaaaaatttc attctctctc ctctggctta atgagccacc gagccgtatc
     9601 cattagctta tctgcaataa agagttttcc cagaccaaca acgacatggt catctttctt
     9661 tatcgttttc atcactttta caccttgcac ggtcaaaaag taattaccat aaattttagc
     9721 aaaagctttg ataggattca tattattctc ctttgatgat ttcagcctct tttcggattg
     9781 tttcaacatc ttcttgatat tgaacttcac tatagttgac tagcttggat aattctttct
     9841 gcaagctttc ccccattggt ttcatagttg caaaggttaa accacctgaa atgataccac
     9901 ccaagatagg aataaatttc cccattcctt tggccaaccc tcccttggta agattcacac
     9961 caaatatttt taagactttt ttcaaaatag ggtaccaaag cgtctttgtt aaagctttat
    10021 taggcactgt tttcattacc tgtttggcaa ttgttatacc accagcacgt agcaaacaaa
    10081 gcagcggttc cattcacccc taacattacg cctagataaa gcaagagggt attttgagca
    10141 tcttcactca actcctcacg tgaagcccaa agatcctcat aaccataaat ataacctaat
    10201 tcttgggcca atttcagaga gaaaccataa aattgagcca cataagctgg aatggtaatt
    10261 gccatagcaa gccctccagg taatcctgcc aaaacagaag tcccactcgc taataaaaca
    10321 ttatccctaa tacaggcatt agccactcga tctaagattt cttgagataa gagagacgtt
    10381 ggaccttgtt ccaataaggt aggaatgtcc tgtggaccca attctttgga aaacttatcc
    10441 actaaaaatt tcgaacgatc aaccttaaca actggtagtt ttaccacttg ttgtaatact
    10501 tgcatagcta agtcttgttc agccatatac aaactcctta tttctttttt gcaccttatc
    10561 atattatatt tcctacaaaa aaagcatttt ctaattagat aacattaaaa aatacagaac
    10621 ccctttttta tagtaaaata aaacaagaac aggacaaatc gatcaggaca gtcaaatcga
    10681 tttctaacaa tgttttagaa gtagaggcgt actattctag tttcaatata ttttaaaaga
    10741 atatttttcc atttcaaatt caacctattt tcctccacca aaaaagggct ctataatatt
    10801 tgtagtgggt aaattcccta tggatattat ggag
//
LOCUS       DAVBBS010000064        10764 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVBBS010000064 DAVBBS010000000
VERSION     DAVBBS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10764
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.064"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            51..233
                     /locus_tag="VQU32_001807"
     CDS             51..233
                     /locus_tag="VQU32_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HET2473213.1"
                     /translation="MKSQKIKVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTIL
                     RLESASIVSELVAGLR"
     gene            533..1372
                     /locus_tag="VQU32_001808"
     CDS             533..1372
                     /locus_tag="VQU32_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HET2473214.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            1390..3228
                     /locus_tag="VQU32_001809"
     CDS             1390..3228
                     /locus_tag="VQU32_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2473215.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPDVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTPAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     NLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            3241..4656
                     /locus_tag="VQU32_001810"
     CDS             3241..4656
                     /locus_tag="VQU32_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET2473216.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSAIPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            5248..6294
                     /gene="pheS"
                     /locus_tag="VQU32_001811"
     CDS             5248..6294
                     /gene="pheS"
                     /locus_tag="VQU32_001811"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET2473217.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            6294..6803
                     /locus_tag="VQU32_001812"
     CDS             6294..6803
                     /locus_tag="VQU32_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2473218.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            6880..9285
                     /locus_tag="VQU32_001813"
     CDS             6880..9285
                     /locus_tag="VQU32_001813"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET2473219.1"
                     /translation="MLVSYKWLKELVGIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEILSCEDVPETHLHVCQVNVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKKFADGIQILPENAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMRGVAHEVAAIYDKAVNFKEFTLTETNEAATDALSVGIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SHVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLSRQVRTIAE
                     GAGLTEIITYVLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLEVTYTATAEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSVIEAALQPAAPFVEITKFPAVSRDVALLLKAAVTHQEVIDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEKKVNA
                     EVR"
     gene            complement(9458..10459)
                     /locus_tag="VQU32_001814"
     CDS             complement(9458..10459)
                     /locus_tag="VQU32_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET2473220.1"
                     /translation="MIKTFLSALSVILFVIPIITYSFFPSSNLNIWLSTRPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIADDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            10615..>10764
                     /locus_tag="VQU32_001815"
     CDS             10615..>10764
                     /locus_tag="VQU32_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2473221.1"
                     /translation="MQDNYTTKGKHLTIDSRRLIERWKKEGKSNREIASLLGKVPQTI
                     HTEIKR"
BASE COUNT         3450 a         1733 c         2287 g         3294 t
ORIGIN      
        1 accccaaaag ttagattttt tctgtctaac ttttggggtg tagttcacag ttgaagagcc
       61 agaaaattaa agtgtctttt actcaatcaa aaatagtaac gcctattctt ttggtgaata
      121 agactattgt ttggtatggt gcaatgccat tattagggaa ggtagatgag atgaccatat
      181 tacgtttgga atcagctagt atagtttctg aactagtggc aggtttacga tagagaaaat
      241 ttttaaaaat ttctatgtat gattttcatt tctttagtga gactgttgcc attatcacat
      301 tcgaatcaca caaaataaaa aaatttctac aagtacttga caaatagatt gaaatatcat
      361 aaaatgaaaa tggttacaga gttattaatt atttaagctt catgtcacca ttaaaaattg
      421 aaataaaagg atgttatcac taatacaagt gagcaggaac ctatttaatc acatcggaag
      481 aagtttcttg atgtttttaa gtaggttcct tttattttaa aagggaaatt ttatgatcat
      541 aaaacgaata ctaaaccaca atgccgtaat tgcgcaaagt aaaaaagata tcgatattct
      601 tctttttgga aggggcatag cttttggaag aaaaactgga gataaagtaa atccaattga
      661 tattgagaaa agtttttttc tcaaaaatag agataatatg acccgtttta cagagatgtt
      721 tattaacgtt cctttggagt tggtgtacat caccgaaaaa ataattaacc taggtaaaat
      781 aacattgggt aataattttg atgaaattat ctatattaat ttaacggatc atatttcttc
      841 gagcatagaa cgttataaag aagggattat tatttcgaat cccctacgct gggaaatatc
      901 gaaatattat aaagaagaat ttgaacttgg gaaaagggct ttacaaataa taaaaaaaga
      961 gttaggtatt gaacttccaa ttgacgaagc tgcattcata gcgctacatt ttgttaatgc
     1021 taatttagaa aataattttc aagagtcgta taaaatcact gaaataatta tgggaattga
     1081 gaaaatcatt caagatttct attgtactga gtttaaccaa gattctattg attattatag
     1141 attcataact catataaaat tatttgctca tcgcttggtt gaaaatacaa cttattgtga
     1201 cgatgatgat gaagacttgt tagcattaat gaaaaataaa tatcctagag aatatgaatg
     1261 tggtgaacag gtggcaatgt ttatacaaac tgagtacaat tatttgctga cttctagtga
     1321 attagtttat ttgatggctc atattcgtcg cttaacaaaa aatttagatt aatagaatag
     1381 gagaatacca tgagttataa agatacggta caaaaaatcc tcgatgtaat tggaggtgaa
     1441 aaaaatgtca atagagttac ccattgtgta acacgtttaa gattagaatt aaaagatgaa
     1501 aatttagtca atgatgatga tgtgaagaag ataccagatg taataggtat tatgaaaaag
     1561 aatggacaat atcaaattat acttggtaat gatgtagcta attattataa agaattcgtt
     1621 aaacttggca attttgaatc cgattcagtt gttcaagggc acaaagggaa tattttagaa
     1681 agaatcattg agtatatcgc tggttccatg actccaatca ttccagcaat gttaggggga
     1741 ggtatgttga aagtcttggt aatcatttta ccaatgcttg gtatattgca atcagattct
     1801 cagactattg cttttttgac attttttggg gatgctccat attatttctt accgctgtta
     1861 ttagcttatt ctgcatcaca aaaattaaaa gtaacatcta cattagctat gtctgtagca
     1921 ggtgtacttc tccatccaaa ttttgttcaa atggtgcaat cagggaatcc tcttagttta
     1981 tttggtgcac ctgtgacacc agctagttat ggttcatcag tcgttccaat tcttattatg
     2041 gtttggttga tgaaatatat tgaaaaaata attgctaaat taacaccagc tattactaag
     2101 agttttttgc aacctacgct agtattatta gtatcaagct gtattgcctt agttgtagtc
     2161 ggacctattg gagtaattgt tggtgaagga ttatcaaatc tagttgggca aatgtatggt
     2221 gtagctggat ggcttacatt agctattctt ggtgctatta tgccatttat tgttatgact
     2281 ggaatgcatt gggcttttgc acctattttt ttggcggcat ctattgctac tccagacgta
     2341 ttaattcttc cagcaatgtt agggtcaaac ttagctcaag gggctgcttc gatggctgtt
     2401 gcattaaaga gtaaaaataa taatacaaaa caaattgctt ttgcagcagg tttctcagcc
     2461 ttacttgcag ggattaccga acctgcatta tatggtgtga ctttaaaata taaaaaaccg
     2521 ctttatgcag ctatgattgg tggtggatta gcgggattat ttgcaggtct tactagtgtt
     2581 aaagcatatc tatttgctgt cccatctttg atagcgttgc ctcaatttat ttattctgat
     2641 gtgccatcaa atattgtaaa tgctttaatt gtggcggtca tttcggttgt tattaccttt
     2701 gtattagctt atatatttgg aatcgatgaa gaagagagtt ctagcaattt agaagttaaa
     2761 gctggagttt caaataaaaa aatgatattt tctcctatat caggagaaat cattccgtta
     2821 agcgatgtcc aggataaaac attttcagat aaattaattg gagacggagt agcgattatc
     2881 ccaagtgaag gtaaggttta tgcaccattt gatgggaaaa ttacaaatat ttttccgact
     2941 aagcacgcaa ttggattgaa gagtgatgag ggtgttgagt tactaattca tattggatta
     3001 gatactgttg agctaaaagg tcaaggtttt attagtcatg tagaagaagg agacagagtt
     3061 ttcaaaaatc agttgatttt tgaaatgaac ttgaatttaa tcaagactaa aggctacgaa
     3121 acagttacac cagtaattgt aacgaatacc aatgattttc tagatgtatt agtattacct
     3181 aataatcaga caatcgagca ttctaaggaa ttactggtaa tattataatg gaggaaagtt
     3241 atgaccattt ttccagatga ttttctttgg ggtggagcgg ttgcagctaa tcaagtagag
     3301 ggagcatata atgaagatgg taagggctta tcagttcagg atgtgttacc caaaggtgga
     3361 ttaggagaag caacagaaaa tcctacagaa gataacttaa aattgatagg tattgatttt
     3421 tatcataaat ataaggaaga tatatccttg ttttctgaaa tgggctttaa tgtttttcgt
     3481 acttctattg catggagtag aatttttcca aaaggagatg aggaagaacc taacgaagct
     3541 ggtttgaaat attatgatga attatttgat gaactacatg ctcacgggat agaaccactt
     3601 gtaactcttt cacactatga gactccatta tatttagcaa gaaaatatca tggatggatt
     3661 gataggagaa tgattcattt ttatgaaaaa tttgctcgaa cagttttaga aaggtataaa
     3721 gataaagtta aatattggct tacatttaac gaagtaaact ctgttttgga attaccgttt
     3781 actagtggag gaatagatat acctaaggag aatctttcga aacaagaatt atatcaagct
     3841 atacatcatg aattagtcgc ctcgagtttg gttacaaaaa ttgctcgtga gattaattca
     3901 gagtttaagg tggggtgtat ggtattagct atgccagctt atccaatgac tccaaatcca
     3961 aaagatgtat gggcgactca tgagtatgag aatctaaatt atctattttc agatgtgcat
     4021 gttagaggtt attatccgaa ttatgcaaaa agatatttta aggaaaatga cattaacata
     4081 gagtttgcag ctgaagatgc agagttatta aaaaattata ctgtagattt tttatccttt
     4141 agctattaca tgagtgtgac tcaatctgct attccaacac agtataattc aggagaaggg
     4201 aatattattg gtggtttagt aaatccttat ttagagtctt cagagtgggg atggcaaatt
     4261 gatccaattg gactacgtat aattttaaat agatattatg accgttatca aatcccatta
     4321 tttatagtag aaaatggatt aggtgctaaa gatcaactaa taaaagatga acttaataac
     4381 ttaaccgtcc aagatgatta tagaattcag tatatgaaag aacatttatt gcaagttgct
     4441 gaagctctac aggatggtgt tgaaattatg ggctatacgt cttggggatg tattgactgt
     4501 gtttctatgt ccactgcaca actttctaaa aggtacggtc tcatttatgt tgatcgaaat
     4561 gatgatggaa atggcacatt caatcgatat aagaaaatgt cctttacttg gtataaagga
     4621 gtgattgaat caaatggaga atccttattc aaatagtttg tttattgatg attagatatt
     4681 atcacggtta aggcaacatc tcattaaata taaaataaag aggtttggaa gacatcaaat
     4741 tctaaacctc tttattttat agggggatta attaatttaa ttttgttaca gagagccgat
     4801 tataaatagt taacttagga tagattggtt atatatagta gactgaatct aaaatagtac
     4861 gaaacaattg ctaaaacatt tatagaaatt aattttactt tcccaatcga tttgttttca
     4921 tcttatttca atccgctata aagttagata agaataagct aacaagcaga agtaagatag
     4981 gtcttgcaag aatggtaaaa atctgataaa ctaagtagta attgaatagg aatctgaaag
     5041 actagtagcc caagggaagt atatcaggga ggagagccgt gactgcaagc tctctatatg
     5101 aaagctgggt gaattcactt gcgcatggat ttagaaatga aggtctgact tgtcagatga
     5161 aaacggatgg taccgcgtgt caacgctccg agtggagttt ttggcatgtg gttttctttt
     5221 tatctacgag agactgatgg aggaaatatg tcaactattg aagaacaatt aaaagcgctt
     5281 cgcgaagaaa cgctggctag cttgaagcag attactgctg gaaatgaaaa agagatgcaa
     5341 gatttgcgtg tctctgtcct tggtaaaaag ggttcgctca ctgaaatcct caaagggatg
     5401 aaagatgttt ctgctgagat gcgtccaatc atcgggaaac acgtcaatga agctcgtgat
     5461 gtcttgactg ctgcttttga agaaacagct aagctcttgg aagaaaagaa agtcgcggct
     5521 caactggcta gcgagagtat cgatgtgacg cttccaggtc gtccagttgc gactggtcac
     5581 cgtcacgttt tgacacaaac cagtgaagaa atcgaagata tcttcatcgg tatgggttat
     5641 caagtcgtgg atggttttga agtggagcaa gactactata actttgaacg tatgaacctt
     5701 ccaaaagacc acccagctcg tgatatgcag gatactttct atatcactga agaaatcttg
     5761 ctccgtaccc acacgtctcc agttcaggcg cgtgctatgg atgcccatga tttttctaaa
     5821 ggtcctttga agatgatctc gccagggcgt gtcttccgtc gcgatacgga cgatgcgacc
     5881 cacagtcacc aattccacca aatcgaaggc ttggtagttg ggaaaaatat ctctatggct
     5941 gatcttcaag gaacgcttca gttgattgtc caaaaaatgt ttggtgaaga gcgtcaaatt
     6001 cgtttgcgtc catcttactt cccattcaca gagccatctg ttgaggtgga tgtttcttgc
     6061 ttcaagtgtg gtggagaagg ctgtaatgta tgtaagaaaa caggttggat cgaaattatg
     6121 ggggccggta tggttcaccc acgtgtcctt gaaatgagtg gtatcgatgc gactgtatac
     6181 tctggctttg cctttggtct tggacaagag cgtgtagcta tgctccgtta tggaatcaac
     6241 gatatccgtg gattctacca aggagatgtc cgcttctcag aacagtttaa ataatgatta
     6301 gaaaagtaga aatggcagat gttgaggtgt tggctaaaat tgccaaacaa gcctttcgtg
     6361 aaacctttgc gtatgataat acggaagagc agttacagga atactttgaa gaggcttata
     6421 gtctgaaaac tttgtcaact gagttgggaa atcctgactc tgaaacctat ttcattatgc
     6481 atgaggagga gatagctggt tttctcaaag tcaactgggg aagtgctcaa actgagagag
     6541 aattagagga cgcttttgaa attcaacgcc tctatgtgct acaaaaattc caaggatttg
     6601 gactaggtaa gcaactgttt gaattcgcac ttgaacttgc tacaaaaaat agtttttctt
     6661 gggcttggct aggtgtttgg gagcataata caaaagctca agcgttttat aaccgatatg
     6721 gttttgaaaa atttagccaa catcatttta tggttggtca aaaagtagat acggattggt
     6781 tactgagaaa gaaattaagg taagaagaga attcttctat tgaaatgata ggaaaggaaa
     6841 agaaccagtg tggcaatggt cattctggag aactaaatta tgcttgtatc ttataaatgg
     6901 ttaaaagaat tggtgggcat tgatgtgcca tcacaggagt tggctgaaaa aatgtcaacc
     6961 acagggatcg aggtcgaagg tgtcgaatca ccagctgccg gtctttcaaa aattgtcgtc
     7021 ggtgagatct tgtcttgcga agatgtgcca gaaactcacc ttcatgtttg tcaggttaac
     7081 gttggcgaag aagaagagcg tcagatcgtt tgtggtgccc caaatgtgcg tgcaggcatc
     7141 aaggttatgg tggctcttcc gggagctcgt atcgctgaca attacaaaat caaaaaagga
     7201 aaaatccgtg gcttagagtc acttggaatg atctgttcac ttggtgaatt gggtatttct
     7261 gactcagttg tacctaagaa attcgcagat ggtattcaaa ttttgccaga aaatgccgtt
     7321 ccaggtgagg aagtcttttc atatctagac ttggatgatg aaatcatcga actttctatc
     7381 acaccaaacc gtgcagatgc cctttctatg cgtggagtgg ctcacgaagt ggcagccata
     7441 tatgacaagg cagtcaactt taaagaattt actttgacag aaacaaatga agctgcgaca
     7501 gatgcccttt ctgtgggcat tgagacagac aaggcgcctt actatgcagc tcgtatcttg
     7561 gacaatgtga ctatcgcacc aagtccacaa tggttgcaaa accttctcat gaacgaaggc
     7621 atccgtccca tcaataacgt tgtagacgtg acaaactaca tcctgctcta ctttggtcaa
     7681 cctatgcatg cttttgactt ggacacattt gaagggactg acatccgtgt gcgtgaagcg
     7741 cgtgctggtg aaaaattggt gaccttggac ggtgaagaac gtgacttgga cgtgaatgac
     7801 ctagtcatca ctgttgcaga caagccagta gcccttgcag gtgtcatggg tggtcaagca
     7861 acagaaatct ctgaaaaatc tagtcatgtt gtccttgaag ctgctgtttt caatggcaaa
     7921 tctatccgta agacaagtgg tcgcctgaac cttcgttctg agtcatcttc tcgctttgaa
     7981 aaaggaatta atgtggcaac agttaatgaa gcccttgatg cggcagctag cctgattgcg
     8041 gaacttgcag gtgcgacggt gcgtaagggt atcgtttcag cgggtgagct tgatacttca
     8101 gatgtagaag tttcttcaac tcttgctgat gttaaccgtg tcctcggaac tgagctgtct
     8161 tatgctgatg tcgaagacgt cttccgtcgt cttggctttg gtctttctgg aaatgcagac
     8221 agctttacag tcagcgtacc acgtcgtcgt tgggatatca caatcgaagc tgatctcttt
     8281 gaagaaatcg ctcgtatcta tggatatgac cgcttgccaa ccagccttcc aaaagacgat
     8341 ggtacagctg gtgaattgac tgtgacacaa aaactcagcc gtcaagttcg taccattgct
     8401 gaaggagcag gtttgacaga aatcatcacc tatgttctaa caactcctga aaaagcagtt
     8461 gagtttacgg ctcaaccaag taaccttacg gaactcatgt ggccaatgac tgtggatcgt
     8521 tcagtcctcc gtcaaaatat gatttcaggt atccttgata ccgttgccta caacgtggct
     8581 cgtaagaata aaaacttggc cctttacgag attggaaaag tctttgaaca aacaggtaat
     8641 ccaaaagaag aacttccaaa tgaaatcaac agttttgcct ttgccttgac aggcttggtt
     8701 gctgaaaaag atttccaaac agcagcagtt ccagttgatt tcttctatgc taaaggaatc
     8761 ctagaagcgc tctttactcg cttgggacta gaagtgacct atacagcaac agctgagatt
     8821 gccagccttc acccaggacg tacagcagtc atttcactcg gtgaccaagt tcttggtttt
     8881 cttggccaag tgcatccagt cactgctaag gcttacgata ttccagaaac gtatgtggct
     8941 gagctcaacc tttcagtcat cgaagctgcc cttcaacctg ctgctccatt tgtggaaatc
     9001 acgaaattcc cagcagtcag ccgtgacgtt gcccttctcc tcaaggcagc agtaactcac
     9061 caagaagtta tagatgctat ccaagctgcc ggcgtgaaac gtttgacaga tatcaaactc
     9121 tttgacgttt tctcaggtga gaaattggga cttggtatga agtcaatggc ttatagcttg
     9181 accttccaaa atccagaaga cagcttaacg gacgaagaag tcgcacgcta catggaaaaa
     9241 atccaagcat cgcttgaaaa aaaagtgaac gcagaagtgc gttaaacgat agtaggatac
     9301 aaattccccc tctagtttat gaacactaga ggggtgattt tatataatac tcaatgaaaa
     9361 tcaaaaagca aactaggaag ctagccgtaa gctgtacttg agtactgtaa ggcgaggctg
     9421 acgtggtttg aagagattgt cgaagagtat aaaataatta aaatgtgatt tctgtaaaaa
     9481 tacatctatg atcagagttt tgaaaactta caatgtctaa atctttaaca tagtagtggt
     9541 ttttaggcaa taaaatatga tctattgttg cattaaaaag ttttggactt tggctattcc
     9601 aagttcctct ttcaaaaggt ggtagtacat ttaatgcgtc cctatgagag cttatttttg
     9661 caagtgctcc atgacgcata gttgcattaa aatcatctgc aataatagcc tttggatatt
     9721 ttgaagccaa ttgattatga atgatgttta agtcttgctt ccagatttcc attaaacctg
     9781 gcagaggagg agcagtatgc aaggcaatga tatctggtat attttgtttt ctcgaatgta
     9841 atacaattgt cccgaaccgt gttgtatgaa aagttttagc ttctgtatag aaaccataac
     9901 ttttcttgac aatcacagtc acaggagcta ttccactatt ggtaggtgga gaagtgaaaa
     9961 tatcatagtt ggccatagaa agtccaactt gatgaaacaa tagtttgatt ctctggtttt
    10021 cttgctcacc tctgatattg gtagctagtt cagggaatat agccatatcg gcgtcaaaat
    10081 ggctaaaaat tcgctcaata tgttgtgctt ctatttgatt agcgacgttc caagttacta
    10141 attttaaggt tttagtctta tttgatgcag aagaaagggt tttatctgtt ccgaataata
    10201 gtaatatgag cgatagtaag agcaaaatgc cagagtaaaa ccgtatttgt ttatttttct
    10261 tgtaaaaaga tagggaaaaa aatacgaaac ttaaaatagc agccatagtt gcagtagcta
    10321 aggggaaggc ataaatctgt gccaagatag gtctggtaga tagccaaata ttaagattag
    10381 aagatggaaa aaaagaataa gttatgatag ggataacaaa gagaatgacc gaaagggcag
    10441 agagaaatgt tttaatcatt gtgtgagtac ctcaatttgc aatggttgct cggagctaat
    10501 gtgactttgt gactttactt gattgatatt ctttttcttg tcttcctcat agattaagta
    10561 caacccaagt aaaaaaagtc caatttaaga ttgaaatgat taaaaggcac agaaatgcaa
    10621 gacaattata ctacaaaagg caaacatttg acaatcgata gccgtcgctt aatcgaaaga
    10681 tggaaaaaag aagggaaatc aaatagagaa attgcctctc tgcttggaaa agttcctcaa
    10741 actatccaca ctgaaatcaa gcgt
//
LOCUS       DAVBBS010000065        10041 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVBBS010000065 DAVBBS010000000
VERSION     DAVBBS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10041
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.065"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(17..1918)
                     /locus_tag="VQU32_001816"
     CDS             complement(17..1918)
                     /locus_tag="VQU32_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HET2473222.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWAYMLNRQEYLLQFMIGYVVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDMLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYYFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYRELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSNKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDICVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            complement(1940..2233)
                     /locus_tag="VQU32_001817"
     CDS             complement(1940..2233)
                     /locus_tag="VQU32_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET2473223.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQTEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            complement(2233..3051)
                     /locus_tag="VQU32_001818"
     CDS             complement(2233..3051)
                     /locus_tag="VQU32_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2473224.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVVIGIIGSWLKIIA"
     gene            complement(3038..3817)
                     /locus_tag="VQU32_001819"
     CDS             complement(3038..3817)
                     /locus_tag="VQU32_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2473225.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTANHVVEEEAHDDWI"
     gene            complement(3832..4323)
                     /locus_tag="VQU32_001820"
     CDS             complement(3832..4323)
                     /locus_tag="VQU32_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET2473226.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLACNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNAPGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            complement(4334..5524)
                     /locus_tag="VQU32_001821"
     CDS             complement(4334..5524)
                     /locus_tag="VQU32_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HET2473227.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSAQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            complement(5536..5964)
                     /locus_tag="VQU32_001822"
     CDS             complement(5536..5964)
                     /locus_tag="VQU32_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2473228.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPVKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEDFESGI"
     gene            6235..7050
                     /locus_tag="VQU32_001823"
     CDS             6235..7050
                     /locus_tag="VQU32_001823"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HET2473229.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            7069..7710
                     /locus_tag="VQU32_001824"
     CDS             7069..7710
                     /locus_tag="VQU32_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HET2473230.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            7741..8742
                     /locus_tag="VQU32_001825"
     CDS             7741..8742
                     /locus_tag="VQU32_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET2473231.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            8752..9381
                     /locus_tag="VQU32_001826"
     CDS             8752..9381
                     /locus_tag="VQU32_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HET2473232.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(9760..10041)
                     /gene="tnpA"
                     /locus_tag="VQU32_001827"
                     /pseudo
     CDS             complement(9760..10041)
                     /gene="tnpA"
                     /locus_tag="VQU32_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         3004 a         2227 c         1834 g         2976 t
ORIGIN      
        1 ttcaaaatgc ctatttctag cactgcaagc gaatcattct ctcatttatt ttatcatata
       61 ctagacattt tccacgcatt tttgttccgt caacgaggca caatttttca cctacacaaa
      121 tatcctcgct taataacaga atcagcttgt ctgtttcgtc atttttcact tcaaaagcca
      181 gagcattttc cacttcacga tcatctgtct ggtagacaga atgacggatg atttcaaaac
      241 tctcatgcgc aatgatggta taatccagca tctgattctc aaagaattgg cgcttggtga
      301 gtttgttact tctttcaagc tcattgtatt taggagaaat gatggtatct tccaaatcaa
      361 aatcaacttc actccatagt ctcaactgat tgattttccc atcttgatag gtcacatcct
      421 tgtcaaggat aaactgagtc aacgcctcat gctgaccttg acacctgatg tcatccacca
      481 agagccagac atcctctacc aacatgagga tttttctctt gtgaagataa ggcaaatcag
      541 gttctgctga ccaataagcc ccttcaatat aatgcactcc ctccctttct ttatggtgac
      601 aaaacaggga gtgaggatag tattcatatt cccaggatcc cgtgattctt tccggagctt
      661 tcccatctac aatgcaggtc gaatgactcc aagcactctt taagagataa cgttcatata
      721 tctcccgata agaataacgc ccagcatcta tgaaaatagg ttgtccttga tactgtaagc
      781 aaaaactatt ctcgtcacta tggctatggg cacttcctag cggaccattt ttgaaaaata
      841 gataacgatg ttcatcctta atgcagacat gtccagagtc ttcaaagatc atggacttag
      901 gctgccaagc tctcttttca aattcctgca gtcgcttgac cttttctcgc cccaggaaca
      961 agaggctaag caaatcgact ttaacatcca gaccgttaag aaggtcttcc ttgttcaaaa
     1021 ccacagcaga caggctcaaa atttctgtcg tttctgtaga atcgctatca ccaaaagcca
     1081 aagttcgtcc atctaagcct gtcatcattt gaatataggt cgccatcttt tccagcaact
     1141 ctcggtaact atcttgcaag tctggaagca agagacacaa atccagcaag gctttataaa
     1201 cctctacatg atagagaatc gactgttcaa actggcttcc atctcctaaa atctgtgtct
     1261 caatttgctg tttcaactcc tctgaagcaa aatggtaagc ttcttctagg tccatcttat
     1321 ctgaaaagaa ataatagata gcaagcatcg gaattgtttg taaaatcccc cagttactaa
     1381 gggtgtactt ggcgcgatag tagcttttca taaagtcaat ctgcttttct agactgacca
     1441 aaattttctc tagttctttc tcctctagca tgtcaaattt caagaggagc aagagtagtt
     1501 tcaaccaagt aaaggaacga atacccgtat ccaaggttct agtcatcaag gattgaggag
     1561 aaaattctct cacctgctca atccaatcaa atagaaagaa cttgcacttt tgaatatagt
     1621 ccttatctcc ttctaccaca taccctatca taaactgcaa gagatattct tgtcgattga
     1681 gcatataagc ccattctgga tcatcttcaa atacttgatc ccataccatc ggctggattt
     1741 gatggatttt tgaacaaggc tccatatccc aaggactatc aaacataaaa cgattgtcca
     1801 tcaagcgttc aagggaactc ttgactttct catagtcttt tgaacagtgc gacaagatat
     1861 aatcacgaca ttggtttcca tcgactcttt caaaaaattg tcttctttct tctttcatta
     1921 tctattacca gaaaaagaac tacttaaaaa gcagttcttt tgtctttccc attacacttt
     1981 ccttttctac atagatgacc acaccttttg caatctgcaa ggagaccaag tcatctttga
     2041 tagaaatgat ttttccatga attccagaca ataacaacac ttcatcacca gatgttaaag
     2101 aagctaaata ctcttgtcgt tgctccgtct gtttgcgaag caacttttgc tgacgaatag
     2161 aatgaaagct tgacagtaaa aggggactca ctgccaagac aatcactatt ccataaaaca
     2221 atgttgtatc cattaagcta taatcttaag ccagcttccg ataattccga tgacaactgt
     2281 taaaataacg agtttatatg ttgtccattt cttttctttg atcaagtagt aaactaaaag
     2341 tgtaaatagg gctggtagaa gagctggagc aaccttatca agcattccct gaatacttac
     2401 gatactttgt ttagcgtctg ctttaacttc ccctgcagca aaggtaatcg gcaccataat
     2461 cttaacagat gtcgctgcca aaccagcaat tacggttaca ccgataatat tggcaatacg
     2521 agaaatcgtt gccatctgtt cgcttagttt atcaatcaca gttgttccta gtttgtatcc
     2581 atacagacca gttgacaatt taatcgctgt taaaatcgta ttcatcgcaa ggaagaacaa
     2641 gattggaccg acaaccaagc cttcttgagc aaacgaagct gcgatggttg agaacaatgg
     2701 agctaaacag aattgagaaa gagaatcccc aatacctgcc aatggtccca tcaaggccat
     2761 cttgatgcta cgtgtttctt ttgccggacg gccattttcc aacattacaa gatgcaagct
     2821 ggtaataaaa ggcaggaagt gtgggttggt attatagaat tcacagtttt cttccaaggc
     2881 ttggtagaaa ccttcctgat cctctccata gtgttttttc aaagcaggat acatcacatt
     2941 ggcatatccc aacccttgat agttactata gttaaatcca ttttgacaaa agaatgcccg
     3001 caaagacgtt ttaagataat cacgttttgt taatttgtta gatccagtca tcgtgtgctt
     3061 cctcctctac cacatgattc gctgtttttg gcttgttata aaattcaatc aaagcaaaga
     3121 tagtacctac aattgcaata ccaattgttg ggatgtttag ataagctgca caaacatatc
     3181 ccaacaagac aaagggaatc aactctttct tagccatcac tgacaagatc atcgcaaaac
     3241 cgatagctgg gagcatttta ccagcaactg tcaaacctgt aagtaatacc ggtggaatgt
     3301 agtctacgag tttcaacaag gtatccattg aaagggcacc caagcaaccc aaggtaaatc
     3361 caataaaggc aaacaaccaa attgttgcat ttagagtaaa cttgaatttc ttcaaattat
     3421 ggtttttcaa gtgcttcatc gccgtttcag gcgcaccagc acgtacagtg taggcgaaag
     3481 tttgtaagaa ttgaatcgcc acagcaatcg gagtagagag ggcaagagcc gcttctggac
     3541 tgactttacc agcactagtg atagccatca aggtaccaaa gataccaggt ccgattgggt
     3601 ttggtggaac agtacctcca gcaccaacac cgaatcccat ataagccaat tcaccaatag
     3661 ctcccattgc aagagcagta ggcagatcac ctagaataat tccgacacca aatgacagaa
     3721 caatacatct attggtgtaa attcctaaca gcattccact aaaacagaaa gctgtccata
     3781 atccaattaa aatcgcttga aatacattaa tcgacataac gatctcctct tttaaatata
     3841 gtccataatg ttgacttgaa cagctccatc atttcctgtt ggagttgttt tcgtattaaa
     3901 tgttacttga tgagtttggc ttaattcctt gagggctgcc ttgtcctctt cacccaggaa
     3961 gatggagcgt gtcacttgct ctttaccagg ggcattgtga atgttcccaa tattgatttc
     4021 tttgataggg acaccacctt ctaccaaggt taaagcgtcc tttacatcct ttacaacgat
     4081 aaagatcgtt tgagcaggat tagccttgtg aatgatatca atcacctttt gcaaagggaa
     4141 gaaacgcatg gcaactgagt ctggcacaac tgttttcatc agagtttgtt gcatcttgtc
     4201 cgtgcttact tcgtcattgg caacaatgac cgtattacaa gctaggtatt ttacccaaag
     4261 ttgtccttgt ccatgaatca accgttcatc gatacgggtc ataataatat ttggcattgt
     4321 catatttctc ctcctaccaa tataggttcc agtctttgta gaaacggata agggcttcta
     4381 ggtaatagta gtcaccccag atattgcctt catccactcc tttacctgaa tgccatgagt
     4441 acacaccgtg gagaagactt gtcccaccag gggtaaattg atcatttgca taatgttcga
     4501 tcaaggaacg aagcatggca tgcatagcat gtttataaat atctttgtca gcatccacct
     4561 ctgggagatg ttttagcatt tcatgaatcc cacagacggc gatagctgtt gctgaagaat
     4621 ctcgtgattg agcactacca tcattaaaaa tcaaatccca ataggacaca tgatcttttg
     4681 gcagacgatt caagaaataa ttggtcacac ccttaaacaa gtcaaagcag gactcgtctt
     4741 ttaagtgacg ataagtcaaa ggaataccat agactcccca tgattgacca cgtgcccagc
     4801 atgaatcatc actataccct tgtctcgtta cacctttaaa gggttgacct gtctcaggat
     4861 caaaatagaa ggtgtggaag gacgaagcgt catcacggat tacattatta gctgaagcat
     4921 agaaatgact ttctgcaata tcgtagtatt tttgatcgcc tgtttcttga taagcaaaga
     4981 ataagagttg gatattgagc aagcagtcga taatcaaacg gtaatgctct ttcttgccca
     5041 agtctcccca agcttgaata aaaccacctt tttcttgata gcgttcaatc aacttatctg
     5101 cagctttcaa ggttgcttct ctagcctctc catctccatt tatcttatat tcagccatac
     5161 aagacggtgt gtacaagaag ccgagatcat ggtgatccaa ttctactctc ttattgacac
     5221 gatccaggaa agaaagaaca tttttatgag cgatgttttt aaatgcatcc tgttgactgt
     5281 attcataagc caaccacagt tctcctgtcc agaaaccatt ggtccattcc gtgttatcca
     5341 tgattggata gacattatca aaggtagctg gcgtcgggaa atcttccttg aaatagtcaa
     5401 ggttgagttc taactgccga ataaccttat cgattgcctg gccgacttct tctttcgtca
     5461 gaagtggtac ttctaagaag cgctcaggcg attttatttt ttcaatcgta acctttttta
     5521 tcattctttt accctttaaa tacccgattc gaaatcttct tcctctgcat ccgttgaaaa
     5581 caattcttta ccatttacga ctccgccctg ggccgccact actgatttat taacaacctc
     5641 atcaaagcta tgagccattc tagcaaagac tgcttccatt aacatggcta agttcaaacc
     5701 cgagagaaca ttcattgttt tgactggatt ttctcccatt atggtagaag aaaccttgaa
     5761 tggcgatcct cccaagagat cacttaggat taaaacttct tcttcatttg aaattgcaag
     5821 taagattttt tgcttgagtt catctgctga cattccttcc acaaagtcaa tcgcctccac
     5881 attttcttga ttacctgcaa tcaattgtaa agaactataa atccctgtag caaaatgtcc
     5941 atgccctaca agtacaattt tcatcttttc tcctttcttg tttaggttta ccggtttact
     6001 ttatattccc cattgtaatc ctttattttt atcttgtcaa tagttttttt aaaaaaatat
     6061 tagcgctttc attatctttt taaccatttt aataaacaga aatttcaaaa cattccgttt
     6121 ttcaggaaaa caaatctttt tataaataaa aatatttatt tattgacaaa accggtttgc
     6181 tagaatataa tttggttata gagaagtctt aacaaatttg aaaggaagta tcaaatgaca
     6241 aatacatcat tctcaattga gcagttttct ttaaaaggaa aaattgctct catcaccggc
     6301 gcttcttatg gaattggatt tgctattgcc aaatcctacg ctgaagccgg cgctactatt
     6361 gtctttaacg atatcaatca agatctggtc aataaaggga ttgaagctta tcgtgaagtt
     6421 ggcatcgaag cccatggata tgtctgtgac gtgacagacg aggacggtat ccaagccatg
     6481 gtcaagcaaa tcgaacaaga ggttggtgtc attgacatcc tcgttaataa cgctggtatt
     6541 atccgccgag ttccaatgtg cgaaatgagc gccgctgatt tccgtaaggt catcgatatt
     6601 gacttaaacg caccatttat cgtttcaaag gcagttattc cttctatgat aaagaaaggg
     6661 catggaaaga ttatcaatat ttgttcgatg atgagcgaac tgggacgtga aacagttagc
     6721 gcttatgctg ctgctaaagg gggcttgaaa atgttgaccc gcaacattgc gtctgaatac
     6781 ggtggagcca atatccaatg taacggaatt ggaccgggtt atattgccac tcctcaaaca
     6841 gcacctcttc gtgagttgca agaagatggt tctcgccacc catttgacca gttcatcatt
     6901 gcaaaaacac ctgctgcacg ttggggaaat cctgaagatt tgatgggccc tgctgtcttt
     6961 ctcgctagtg atgccagcaa ttttgtcaat ggccacatcc tatatgtaga tggcggtatc
     7021 ttagcctaca tcggaaaaca acctgagtaa aaatagaaag aagatcttat gaaaatcgca
     7081 ttaatcaatg aaaatagtca agctagcaag aatcacatta tttacgatag tctaaaagaa
     7141 gcgacagata aaaaagacta ccaattattt aactatggta tgcgtggaga agaaggagaa
     7201 agtcaattaa cttatgtgca gaacggacta atggctgcca tccttttaaa tacaaaggca
     7261 gttgactttg ttgttaccgg ctgtggtacg ggtgtagggg ctatgcttgc tttaaacagc
     7321 ttccctggtg ttgtctgtgg tctagcagtg gacccaactg acgcttacct ttattctcaa
     7381 atcaatggtg gtaacgcctt gtctatccct tatgccaaag gatttggctg gggggcagaa
     7441 ctgaccctca aattgatgtt tgaacgctta tttgctgaag aaatgggcgg tggctaccca
     7501 agagaacgtg taatccctga acaacgcaac gctcgtatct taaacgaggt gaaacaaatc
     7561 acccacaatg atttgatgac catccttaaa acaatcgacc aagacttcct caaagacacc
     7621 atctctggca aatacttcca agaatacttc tttgaaaact gccaagatga tgaagtcgct
     7681 gcttatttga aagaagtatt agccaagtaa agctattcta aaccagaaag gaactaatgg
     7741 atgacgaaaa tattactgtt tggcgaacca ttaattcgaa tctcaccatt agatgccacc
     7801 agtatcggcg atcatgttgc cagttcgact tattttggcg gatcagaaat taacatcgct
     7861 tgtaatttgc aagccctggg tatctcaacg aaagtcttta ccgcactccc tgccaacgag
     7921 attggagatc gttttctcac attcttgaaa cagcaccaaa tcgataccag ttcaatctgt
     7981 cggcttggcg atcgaatcgg cctctactat ttggagaacg gctttggttg tcgtcaaagt
     8041 gaagttttct acgatcgtaa gcatacgagt atcagccaga ttcggccaaa catgctagat
     8101 atggattctc tctttcaggg gattagccat tttcatttta gtggaatcac catagctatc
     8161 ggtcaagagg tccgtgcgat ccttctccta ctcttggaag aagccaagcg ccgaggaatt
     8221 gtcgtttcaa tggatctcaa tctgagaaca aagatgattt cagtcctaga agccaagtat
     8281 gaattttcta agtttgcacg ttttactgac tattgcttcg gtattgatcc tctcatgatt
     8341 gatgaccaaa atctagagat gtttccaaga gacagtgcta gcctagaaga ggtggaaaat
     8401 cgcatgcgac ttttaaaaga agcctatggt ttcaaggcca ttttccatac cctccgatct
     8461 agtgatgagc aagacaaaaa tgtctatcaa gcctatgctc tggaagaacg atttgaagag
     8521 tctgtccaac taaaaactgc agtctatcaa cgaattggta gcggggatgc ctttatatct
     8581 ggtgcccttt accaactact ccatcattcc tccctaaaaa ctaccattga ctttgcagtt
     8641 gcgagcgcaa ctctcaaatg cactcttcca ggagaccatc tctccacttc cgcaactagt
     8701 attgaaaatt tactggcaaa tgcacaagat atcattcgtt aggagaatta catgaccaaa
     8761 tcagatacga ttattgaact aaaaaaacag aaaattgtcg ctgtcattcg aggaaataca
     8821 aaggaagaag gactacaagc ctcgattgct tgtatcaagg gcggtatcaa agctattgaa
     8881 atcgcctata ccaatcagta tgcaggacaa atcatcaagg aacttgtaga cttgtatcag
     8941 gacgatcaga gtgtttgtat cggtgcaggt actgtgcttg atgccgtaac tgctagagat
     9001 gccattctag ctggagcaaa ttacgttgtt tctccatctt tccatgctga aactgcgaaa
     9061 atgtgcaatc tctacagcac accgtacatt ccaggctgta ttaccctcac agagatcacg
     9121 actgcacttg aagccggtag tgaaatcatc aaactcttcc caggtagtac tctcagtcca
     9181 gcatatatct ctgcagtcaa ggcaccgatc ccacaagttt ccgtaatggt aaccggagga
     9241 gtcggcctaa acaacatccc tcaatggttc gctgctggtg cagatgccgt tggaattggt
     9301 ggcgaactca ataaactcgc ttcccaaggc aactttgacc gcatcagcga gattgcccaa
     9361 cagtatatta cactcagata aaatcataac tacccgtcta acgggtggtt tatcccagag
     9421 ctataagccc aaatcatcag ccagcgccta aagacgctgg cttttacgtt gttcaagcct
     9481 tattgctctt gactcgtcac ttgcctcttt aagagacttt ggtattactt accactatcc
     9541 ctaaagggat cctcatattc ttttacactc aatttatcta gtgctatagt agattgaaac
     9601 tggaatagta tacctctact tctaaaacat tgttaaaaat cgatttgact gtcctgatcg
     9661 attttgtcct gttcttattt cattttacta tatatcatac tttactagtt ctcaaatttt
     9721 catactcgtg aagaaatcat ccactggata atttctttaa tcttgactat atttcttaat
     9781 tgtggcttca ttaagcccta ctggacttac ataataacct tccacccaga aatgccgatt
     9841 cccaaacttg tacttgagat tggcgtgttt gtcaaacatc atgagtgcac tttttccttt
     9901 taaataaccc atgaaactcg aaacacttag ccttagtgga atactgacta acatgtgtac
     9961 atggtctggc attaagtgac cctcgatcat ttcaacacct ttataactac acaagcgatg
    10021 aaatatttcg cctaaactac t
//
LOCUS       DAVBBS010000066         9999 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVBBS010000066 DAVBBS010000000
VERSION     DAVBBS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9999
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.066"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(108..2213)
                     /locus_tag="VQU32_001828"
     CDS             complement(108..2213)
                     /locus_tag="VQU32_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2473233.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELERKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQEKVIGQDKAVEVVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVVIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            complement(2822..3802)
                     /locus_tag="VQU32_001829"
     CDS             complement(2822..3802)
                     /locus_tag="VQU32_001829"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET2473234.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNMGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFGYQVHLGIFYIVFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLSFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            complement(3804..6056)
                     /gene="pbpX"
                     /locus_tag="VQU32_001830"
     CDS             complement(3804..6056)
                     /gene="pbpX"
                     /locus_tag="VQU32_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HET2473235.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDSFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAVYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKVEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            complement(6060..6377)
                     /gene="ftsL"
                     /locus_tag="VQU32_001831"
     CDS             complement(6060..6377)
                     /gene="ftsL"
                     /locus_tag="VQU32_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HET2473236.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            complement(6389..7339)
                     /gene="rsmH"
                     /locus_tag="VQU32_001832"
     CDS             complement(6389..7339)
                     /gene="rsmH"
                     /locus_tag="VQU32_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET2473237.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            7505..7699
                     /locus_tag="VQU32_001833"
     CDS             7505..7699
                     /locus_tag="VQU32_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2473238.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            7715..8257
                     /locus_tag="VQU32_001834"
     CDS             7715..8257
                     /locus_tag="VQU32_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HET2473239.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIGNQVFIVLFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            8268..8510
                     /locus_tag="VQU32_001835"
     CDS             8268..8510
                     /locus_tag="VQU32_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473240.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            8521..8856
                     /locus_tag="VQU32_001836"
     CDS             8521..8856
                     /locus_tag="VQU32_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473241.1"
                     /translation="MKSLARLLNIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(8966..9967)
                     /locus_tag="VQU32_001837"
     CDS             complement(8966..9967)
                     /locus_tag="VQU32_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2473242.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
BASE COUNT         2876 a         2269 c         1785 g         3069 t
ORIGIN      
        1 attgaaactg gaataataca cttctactat acgacatgcc tgtcgtacct aaaaaggaac
       61 cagttttttt cagctggctc cttctttttt atcctcaaaa ttctatctta ggctttctca
      121 cgaataacca aaacgccatc ttccatatct gcttccagat gtttagcatc taaatgatcc
      181 aagtggaagt ctgtcacctt atcacgaatt tcttgttcaa ccacgcgacg gagaggacga
      241 acccccatga cttcgtcgta accttcttct gtgatataat ctttagccgc ttgactaact
      301 accaagtcaa tgtctttctt agccaaggtt tggttaactt cagccaacat caaatctaca
      361 atcttagaaa ggtcttcctt agtcaagtgt gagaactcga tgactgcatt aaagcggttg
      421 aggaattctg gacggaagaa gggtttcaaa cggtccatca attctggttt atccgcatct
      481 tctgtcaagt tggcttcata gccaaatcca gcatttgagg tcgcaataac gacagtattc
      541 ttgaagttta ctgtatttcc ttgaccatct gtcaaacgac catcatctag aacttggagg
      601 agaagggtaa taacttgagg atcagccttt tcaatttcat ccaagagaat gatagagtat
      661 ggattgcgac gaacacgttc tgttaaggta ttgctattgt catcataacc cacatagcct
      721 gctgttgtac caattagctt agaaacagct gtgcggtcac tgtattcaga catatccaaa
      781 cggataatcg catcctgggt tccaaacata tcgagtgcca attgcttagc aagctccgtc
      841 ttaccaaccc cagtagaccc tacaaagagg aagctgccga ttgggcgatt tccttcatca
      901 aaaccagcac ggttacgacg gatagcacga gctacaactt ctacggcctt atcttgacca
      961 atcacctttt cttgcaagcg atgagccata tctttcaaac gttcgatatc tgaagctccc
     1021 atttgcgata ctgggatacc tgtcattcgt tccacagatt cagccacatc gttgacactt
     1081 gcagtcactt tcatatcttc tgtgtggttt tcgattttcc tttccaattc tgcaatgcgt
     1141 gttttatagt ttagagctgc ttcaaaatct tctgcttcaa ctgctttttc ttgcttgtct
     1201 ttttccgttt cgatttctcg ttcaacagca tgcacatctg ttactggatg ttgagccgcc
     1261 aagtgagcag ccgttacatc gacaaggtca atagccttat ctggcaagct acgttgagga
     1321 atgtattgaa cagaataatc caccgctgct ttcaagactt cgtctggcaa gatgacattg
     1381 tggtgttgtt gatagaggtc acgaatgcct tgaagaattt taaaagtatt ctctgctgaa
     1441 ggagcattga ccttcacttc gttgaaacga cgagcaagag cagcattctt caagatggtg
     1501 ttacggtatt cgtcttgagt tgttgcccca atcactgtca attctccacg agagagagct
     1561 ggcttgagaa tatccgcaag tcctttagaa ccactgtctc caccagtgct accagcacca
     1621 agaatttggt gaatttcatc aaagaagagg ataatattcc ctgcttcttt cacttcattg
     1681 actaagtttt ggacattttc ttcaaagcta ccacggtatt gagtaccagc ctcaagacct
     1741 gagatatcaa tggaaataat ttccttgttc ttgatagcag caggaacatc tccgttcaca
     1801 atggcttgcg ctagaccttc gacaactgct gtcttaccaa cacctgcatc tccgaccaaa
     1861 acaggattgt tcttggtgcg gcgtgagagg atttcagatg tttcttgaat ttccttgttt
     1921 cgtccgataa caggatccaa cttgccctca cgcgcttctg ctgttaagtt tcgacctagt
     1981 tttgcaagga caccgtcttg tttcatacct gatgcctgtt gtggcatttg cacatcagtt
     2041 tctgcatttc ctggtaattg accagtcgca cgatagtgag caaattcctc aggtgtgact
     2101 tcgcgtccat taattaagta acggcgattt tcagaactat atcctcgcat accacccatc
     2161 aattggttaa ataaatcatc catgttatta aaattattaa agttgttatt catattcttt
     2221 acctcttttt gtttacttag ttatgattac tgatattgac tatctttgac cattgtttta
     2281 aaaaatttag actagctaga tcgctaccct acgtactatt tctggttttt ctttttcaag
     2341 caggagtaga ctatctttac ctctgaagat tgctagatga aacatagact cacctctttc
     2401 tattttactt taaataagtg ttctagtaag gctttcattt accttacgtt cataatatac
     2461 tacattggtc agaaaaggtc aagagttttg actttaactg accaatattt ttttacactc
     2521 ttcgaaaacc tcttcaaacc acatcagctt cgccttgtct acaacctcaa aacagtgttt
     2581 tgagctgact tcgtcagttc tatcaaccat ctcaaaactg tatagtaaaa tgaaataaga
     2641 acaggataaa tcgttcagga cagtcaaatc gatttctaaa aatgttttag aagtagaggt
     2701 gtactattct agtttcaatc tactatattt tgagcagcct aaagttagct ttctagtttg
     2761 ttctttgatt ttcattgtgt atttaaacac aaaaacaccc tgaaacatca gggtgccatt
     2821 cttacatcaa atacaaaatt gcaagggtca ggagacttgc tagaagccca actccccaaa
     2881 agaagaagtc aaccttccac tcgctccaag gatttccttt accagacaat cccccaagct
     2941 caaaatggtg atgtacaggc gtcatacgga aaatacgttt accacctgtc agtttgaaat
     3001 aactgacttg catcataaca gaagttgttt caaaaacata tacaattccg ataatcaaga
     3061 gagtccattc ttggtggaga gccatagaga tagctgccag catcccacct agggccaaac
     3121 ttcccacatc acccataaag accttagcag gcttatggtt aaagatgaaa aaactgagca
     3181 aaccaccaat catggcaaga atcactagaa gaatatccat ctgaccttgc acataggcaa
     3241 taactccata ggcagacaaa ctaatcacaa cggaaatact agctaaaccg tcaacaccgt
     3301 ctgtcaagtt tactgcgttt gaaaaaccga ctagccagaa aagagcgaag acaatataga
     3361 aaatccctag atgcacttga taaccaaaga cagaaagcat atcgccaccg cgctcataga
     3421 aaagatagaa gataactcca cctagaagct gaagagctaa tttttgctta ggattaagcc
     3481 cctcattgat tttacgaaag accttgagaa agtcatctaa aaatccgacc aagccataca
     3541 agaccaagat gaacaaaatc attcccatat tattgctgaa ttggctacta aatagggcga
     3601 aaaagaaagc aaccaaaaca gaagtaatca agaaaaccaa acctcccatt gtaggagtcc
     3661 cagcttttgc ctgatgctgt ttgacatcct catgcatctg ctggcctgta atttgcgcct
     3721 ttctataaaa ttggataaag gccggaattc ctactaaagt tagtaaaaat gtcacaattc
     3781 cagcactgat ggaaataaac atattagtct cctaaagtta atgtaatttt tttaatgtcc
     3841 ttgatagctg tgttagcacg aacatcttgc ttctgcacag tagagcccga accttgaaat
     3901 tcaagttcta tattgagcca cttagcaagg gtctcagcag tctcctttgt ccaaccatac
     3961 atatctggaa cttcctctac tttatcagat aagataagga cttgctgatt cggggcaaga
     4021 ttcttccctt cttcagcaga actgttttta atcttcgttc ctgttcccac aacgatgggt
     4081 tgtacaagat tgcgacgcaa ttcttctgct aaatcaccag gtgaaatatc cttgacacta
     4141 ggcataggat aaggactttg ttgacttact tgctccaaag ctttagctgt tgtttgaaga
     4201 ttgagagagt ctttcatagc tgaagcccgc tccaagatag gattggcaaa ttctcccaac
     4261 tgaatacctg aataatgttc aggttgttgg accgtcacat acaagataaa atcaggattt
     4321 tcagccggac tcatcgatac agccgagaaa atatagtcgg ttaacccgac tagataacca
     4381 ccatttttct cgtcagcaat ctgagccgta ccagacttga gggctacatt ttgcccagga
     4441 acagttacag ttggcttgcc tgtgctgtgg ttatacatgg ttccataaac cggatccgtc
     4501 cctaccaaaa ccatgttagt ccgagttaga ctagctgcat ctttagaaac aggatttccc
     4561 acaatttctt tttgagattt ccgagcagtt tgatcatttg gatcataaac ggcactaata
     4621 aatttaggct ccagcatgac accgtcatta gcaatagctg taaaggcacg aatcatttgc
     4681 gtctgggtca ctgaaatccc ttgtccaaat gagctttgcg caatgttgac aatattatcc
     4741 gcaggaagct gaccagcata ctcatccgtc aaaccgaaac gggtcggaac tccaaactta
     4801 aaacgattaa gataatcaag ccaggtagca tctcccatct tttgctcaag gagggtcatc
     4861 ccaacgttac ttgagtgtgc aaaaccttga gaaaaagtca tcattctgcc accagtcaat
     4921 ccttcattaa cgtcccaatc tcgaatcgtg gcatctgcaa tttttaactc actactatta
     4981 aagacttctc ctcctggaaa ggtattatta tcaatagcag cagccaacat catcactttc
     5041 atagtggaac ctggctcata gttactttgg taaaggatat cacgccaaac aaagtcctct
     5101 gtaatgcctt cttttgtatc tgcatcaaag gtcggtcgtt gcgttgttgc cagaatttcc
     5161 cctgttttag cactgaccaa agtcgctgtc atgtactttc cttttacctt ctcttgaaaa
     5221 gaatccatct gggtttccat aaaggactgg agggggctgg aaatggttgt ataaacatcc
     5281 ttaccgtcca tcgttcgttg ggaaacttgt tctgttccgg gtacaatatt acccagacga
     5341 tccttttcat aggtaataat gccgtctgtc cctgcaagaa tactgttcaa ggaactctcc
     5401 attccagagg ttcccagcaa gctcttgctt ccatcttcat tttcatggag ctgagctagg
     5461 ccgataaaac tagaagcaaa ttgtccgttt gggtaactac gattgggact ggttgtaaaa
     5521 tcaatcccct tgacctctgc agcttccaat tcttttttga tagacatcat attggcatag
     5581 gtaatcccat ttccctttgc tccaaaggaa acttgcttga gattaggttg cgagagttgc
     5641 tctcttacat aggattcttc catgtccaga tacttatgaa agacctctgc aaccttgtta
     5701 aattgtgttt tttctacgta aagaatctta cccgttgctg acttatagtt ctcatcaatg
     5761 accgcataga cattatagga ggttgcatcc tcagcaatcg ggactccatt tcggtcataa
     5821 atagtcccac gtttggcagg aactgtacgg gtggtttgat gaaccttctt agcttccttc
     5881 gctaaatctg ttccaaagcg agtgcctgtc ccaataatga ccgcaaaatt gactaaaaaa
     5941 atggcaaaaa caaagacaga taataaactc agactttttc caactctgcg tctgttttca
     6001 gccggcgatt tccgattttt ggtcgcataa cggattactc tttttgtcca cttcatatct
     6061 tactccgcta ttctaatatt ttcattgttt aattgcaaat cgtgtgaatt ggcaatttct
     6121 ttcaaacgtt ctgcacgtaa tagttcattg acctcttgct tggcatcgtc caattcggtc
     6181 ttcttttcct ctatctgcgc attgattttt gtcaaatcat tctgcacttg caagagcttg
     6241 gtctgcataa aaataatact aatggctaca ataagagtgg ttacagcaat ggaaaagtaa
     6301 aaagcttttt ccacacgcga aaaccgttta agttgcatct gtagtatttg acctgttttt
     6361 tccatttttt ctgccatctt tttccctctt acttgtgaat ttttctggcc acgcgcaact
     6421 tggctgagtg cgagcggtta ttggcttcta actcttccgc acttggcaag attggcttac
     6481 gggacaccaa ttccatcttg ggcttgagat catctgggat gaaaggcaag ccttttggaa
     6541 cttcaactgt tgaagcttcc ttgaacaatt gcttggtcaa gcggtcttct aaggaatgaa
     6601 aggtaatcac tgaaattcta ccatccagag ccaacatatc catagcctgc tggatggact
     6661 catctgccgc tcccagttca tcattgactt caattcgaat agcctggaaa atctgcttag
     6721 caggatgacc cttcttcttg agttccttgg caggtttgac caacttgata atctctgcta
     6781 actcagtcgt tgtctcaatc ggcttcactt cacgcgcttg ctcaatctta cgcgcaatct
     6841 gtttagagaa tttatcctca ccgtatttga agaaaatacg aaccaaatca tgatagtcat
     6901 agtgattaac cacttcatag gctgtcagac tagcatcctg attcatccgc atgtccagtg
     6961 gcgcatcctt tttataagaa aaaccacgct cacgctggtc caattgagga ctagacactc
     7021 ccaagtcata acaaattcca tcaatttcct gaacaccagc ttcgcgcaaa cgtgcctgta
     7081 aatgacggaa gttatccttg ataaaggtca ccattccctt ctcaatgtaa ggtgccaagc
     7141 gtttttgcgc attgtcaatg gcattctgat cctggtcaaa ggcatagaga tggccttttt
     7201 cacttaattt acttaataaa tactcgctat ggcctgctcc acccaaagtc gcatcaacgt
     7261 agataccgtc aggttttacg tcaagcatat caatcgtttc gtggagtaag accgttacat
     7321 gatgaaattc ttttgtcata tcttatctat tttaccacaa atccgaccag cttgcacttg
     7381 tcagacaaat aggacaagga gactactatt tcttcaaaaa atatgtcaaa tatacttgac
     7441 actttatgtt taaaagacta taatatagtc aaatatatac gacataaatc agaaaggaga
     7501 ccagatgaat cgtgtgaaag aatttcgcaa ggaattgggt atttcccagc tcgaactcgc
     7561 caaggatatc ggtgtctcga gacagactat caatatgatt gaaaacgaca agtacaatcc
     7621 aaccttggaa ctctgtctca atctcgcccg cagcctccaa actgacctca acagtctctt
     7681 ttgggaggaa gatttttaaa aaaggagcaa actcatgaaa aaagaaacct tcactgaaaa
     7741 actgatcaaa cgcacatacg gtatttctgg tccccttgac gaatacaaac ggcgtgaggc
     7801 cgatagtatt gggaaccaag tctttatcgt cctcttttat ctgatgattt tcggaaatct
     7861 tattccactc cttctagcct ataaataccc tcaagaagtg gctctaatct atcctcctct
     7921 gattttagtg attgccctca tcgctgctgg ctatgtcacc taccaaatga aaaaaacagg
     7981 catcacagtc attgaaccag atatgctgaa tgagaaagaa agcaagcaac tacactaccc
     8041 aggtctgaaa gcaggtttgt tctttggtct atggatgttt tttataactc ctcttctcag
     8101 tatactcata gatgaaggtc aggactattt tcattctctt ctcactataa gaaatggtgt
     8161 atcaagtatt ctcggttcta ttttcttcgg agcgagcata cagttcctca tctcccgtcg
     8221 cattgcaaaa actaagaaaa atcaagatga ggattaggag gaaactcatg aaaaaagaaa
     8281 agaaacagtt acgttatcct ggtctgaaag caggttcgat ttatggaaca gtgatatttt
     8341 ttataattcc actcattgat accctaacaa gtgaaaatcc aaattttatc agttctcttc
     8401 taaatacaaa acatattttt aaaactatac tgggagcttt ctttttcgga gtgatgatac
     8461 atattgtcga ctcacttcgt attgcaagag ctaaaaaaga ccaggattag gaggtgcctt
     8521 atgaaatcac tagctagact actgaacatt catgttttta tcagtatttt ccttttcttc
     8581 gcccttattt caggagctgt ttctcataca gttttactac tcctactcct ctttcttcct
     8641 gcgctcaata aaggacttga gaaaatacaa tcaaaacgga tacctgtcct caacgcagcc
     8701 ctcttctttc tcctcatatc ctttccacaa cttttaacca accctgtcca atggaaattt
     8761 tcaatattcc tagtcgtaac catcatttca agtttggcct acttctataa cttttatcaa
     8821 gtagttaaag aagtagatca aaaacagttg atttaggagc ttgctcccat aatttctcat
     8881 ttcataagca aataatccca cctagcttgt tctaggtggg attattttat ttcttaacag
     8941 agattgcaag tcattttcct tcattttaga aagtcgactc tttccaattc acactacaat
     9001 ccaagacttg ttgcctttct tcttgattac ggccttcaat ctgatcaatc aaaatctttg
     9061 tagcctgttg tccttcctca aaggagggct gaaccagcgt cgaaacactt ggagaagaaa
     9121 agcaagtcca ctccgtattg tcaaaaccaa tcaacccaac ttgtggcaag ttataattca
     9181 actctttgat aacggtaaag actagaggta gggcccaaca gttagggata aataccagag
     9241 ttttttcatc gggatcgatt tctttttgta aaaattcctt aatttgttcc aaattcgtat
     9301 gcttatcttc aatggttaga ctggcgtgac gcatattagc atctgttaaa gcatccacaa
     9361 aaccacttgc ccgctcaatc cgagtactca aacgactcgt atccgctgta atcaagagaa
     9421 aatgctcata acctttttcg atacaggact gggtcatgtc ataaacggca tcatagttat
     9481 tggttttaac ccagctagtc cggtgttcat agagctgact atcaaaaaag accattttct
     9541 tctttttctc atcgatgata cgagaatatt ttcggaaatt agaggtcggc tgaataataa
     9601 agccgtctac tcccaagaga agcatgcttt caatataccg gtcctcactc tcttggctgt
     9661 aattactatt tcctatcatt acctggtagc cattctggct ggcgatatcc tcaattccct
     9721 taacaatttg gtttgagaaa ctgttggtaa tatcaccgat caaaacaccg attaattttg
     9781 ttcgtttgga gtttaagcta cgcgcaacaa tgctcggttt gtaatttgtt tcatgaataa
     9841 ctttttcaat cttttcacgt gtctcttggg acattttttc atatttcccg tttaggtaaa
     9901 atgacacggt tgttttcgag gtctgagcca tttccgcaat gtcttttatg gtcagtttct
     9961 tctccaatct aaaacacctc ctactaatac ttattataa
//
LOCUS       DAVBBS010000067         9987 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVBBS010000067 DAVBBS010000000
VERSION     DAVBBS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9987
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.067"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..77
                     /locus_tag="VQU32_001838"
     CDS             <1..77
                     /locus_tag="VQU32_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2473243.1"
                     /translation="MPQSPWVERIVQVLDLDYEDLFRR"
     gene            80..667
                     /locus_tag="VQU32_001839"
     CDS             80..667
                     /locus_tag="VQU32_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2473244.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQGSTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHTGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNLLVYSLLLF"
     gene            869..1006
                     /locus_tag="VQU32_001840"
     CDS             869..1006
                     /locus_tag="VQU32_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HET2473245.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
     gene            1185..3794
                     /locus_tag="VQU32_001841"
     CDS             1185..3794
                     /locus_tag="VQU32_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HET2473246.1"
                     /translation="MDYNFNLEHPFFFTNNDYSTDTSIKYQVSLPFNWHEVMNNDEWV
                     YQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICHEMRIPFKHLSTEDKFIMRNGK
                     LVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGPYILSDKRWDEAPIYLRYGVFR
                     PSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVPPDFLNKWLDKKDKKQGDFPFI
                     IDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLGFDGTYSSEKLASECKALKILN
                     EWSETPKIYWHGKIWEHTFLGIEHMKGVPLNRWVTNNFPLYEVVDKTKDYLLRVSKIV
                     EKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIIDWEQAVFSNDEKVVHKVAAPGF
                     RAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNYVSQTRIEGKKLFESLGYTREH
                     IDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIESEQDIQDFIIKLLRGFTLTYG
                     QWRKEFQSRFFPVHYYGLNFNQGIAFSDLAILWSYQQLAKKVKNFKFDDYYEIRTQVI
                     NEAVNNFKKSSLSGLFDGKIGTIWLIYEFGEIDRAVELFTTHFIEIFENSQNKNLYSG
                     QAGILLVGLYFLSKGEIDNKLGEEILIRLREYTLNYIENPETFCKVGASDVQSNDPYE
                     NFGGLLYGHAGVAWLFGEAYKLTGESIYKNGLELAVDKELVAYKVDSNNSLQYSQGHR
                     LLPYLATGSAGLLLLINRNKEILSSKYLKYLTSLERATDVVFCVLPGLFNGFCGLEVA
                     NNIYSDIDDNFSGQKKLIEQLYRYLCVIEEGFVIAGDNGLKITTDIASGFAGVAIGLV
                     SIMDNKLTILPQI"
     gene            3827..5767
                     /locus_tag="VQU32_001842"
     CDS             3827..5767
                     /locus_tag="VQU32_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET2473247.1"
                     /translation="MNQSNEIFLNTIQEKTHYKRSISSSFQELKKWNSLIFRRGRYWY
                     QFDNWSIPNTGVIKEWYVEDNDWIPKVSSSSLDNSNSLKIVFDVEKYTLPDDSILQDV
                     RISSDESYIVLVVSSLRSTNLIGIKKYTMEELFIIEDISVESSFYLGKFGVMYTRSKE
                     YGRPSKLFYKSFDSFTEEELFEENECSFRLKIVHIDSNNCFVKSVDFQKGRIFLYSFD
                     RTGFVRHSYTETVAPTPRDIALFSTEKAQYFLGLSSTEEKKDQLILRETSSGDRVSIT
                     IPYQDRARRVHCIGRYILLDCSNAANSVFYLATFKNNSLRELSVNKITFDEKTTLYEN
                     SFSDRVLLFLERRTFYEKILSFDLIENTLKTEFERPIIKSNNTKYFSKVIWTKKDSYD
                     VSIPISLFWKSEDTDELPRRKKCILSVYGAYGKNDNSDLDEIMLSIIDAGFIYAIVHV
                     RGGGYLGGEWYRSGKALNKWNSIRDFIEGVNYLRENDVIDSKRLGLITSSAGGIIAGA
                     VLNEEKNLLQSILLFSPFINPYDTLQNPNDPLSKTEIAEWGDIRDPEVKAYIKSYSPM
                     QNIEKARDSNTVIVNILGEKDPYINNNEVIEWSKKLNSIGVKSLLYLNKAAGHGGFTP
                     SDVLLMIDTLNYFFEEVGRNNL"
     gene            5764..7371
                     /locus_tag="VQU32_001843"
     CDS             5764..7371
                     /locus_tag="VQU32_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2473248.1"
                     /translation="MNYLKFIKKTKLILMGILIFLSSFNGVLLSGIIVYAGSLNQTSS
                     FSDVLRFGAISILGWSAIYISNYYLEVTEASITKDINVKIKQGYFREQYLSSEMVKDY
                     SSIISVLSNDLRLIEENYFRQIFEIISSILLFIVSLSFMLYLNFLVSIIFIVLSALPI
                     IVPVFMKKMLSNSANEYSNSNAEYTHIIKEIFNGFKTLKSYSVTKEIISLSDKKLDKL
                     EDSTFNLKRSEVLSKLVAVLISGFCFLVPLVVGCYFVIYHKSLSFSELIGIFLANDKV
                     LGPIQSIAYSLNKINTTKDLRKPFLKYLSGEKNFIDAEHDNNGLYTSSIDEIHMKDVV
                     YSITPENKLSIDFSFKSPFRVLLTGTSGSGKTTILNLINGSLKPQKGYVNLLSHGKKS
                     SDSIPTVDQTPYIFDTTIRENVTLFQNEYFSDDQIIEVLKKVNLYEELEKIDILNYQC
                     GENGSNLSGGQKQKIALARALIRNNKVYLFDEISANLDNDNSNSIHDILFNLGISFIE
                     VSHHYDLNDKRYTDIYKLENGTLFKIK"
     gene            7386..8126
                     /locus_tag="VQU32_001844"
     CDS             7386..8126
                     /locus_tag="VQU32_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2473249.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            8129..9448
                     /locus_tag="VQU32_001845"
     CDS             8129..9448
                     /locus_tag="VQU32_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473250.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASLLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVIIYQLSPFIQSCFIKLLSIIIFRNNFMFIASKWNLLYRKPY
                     IKSISAAITGAILLISSFQMISQNILSQFQDDSDLELKVAFIVYVGAPILIVLANIIS
                     IAFLSSHQERIEIQQFEILGTSNYQMVKIKVGEAIFLTFVTSLIAFLLNIKIMALIYY
                     SLEDVLIDDMNLLGLILPNFIVSIILFILIFITKSSYFIFKNAKIIS"
     gene            9582..9719
                     /locus_tag="VQU32_001846"
     CDS             9582..9719
                     /locus_tag="VQU32_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HET2473251.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
BASE COUNT         3389 a         1244 c         1910 g         3444 t
ORIGIN      
        1 gaatgcctca aagtccatgg gtagaaagga ttgtgcaggt attagattta gattatgaag
       61 atttatttcg gaggtaacta tgaaaagaat aattccagtt tatatattcc aacaagtaaa
      121 tgtcctattg gtatctctat acttattgaa atttctttgt atcggtgagt taactatact
      181 acagattctc tatggttcgt cactcatttc ttttttatgg atgtatggcc aacgcaaaca
      241 agcgcataag gtcaatatga aatctaggat gaaatggctt ggtgttgaat ttgttagcct
      301 actgattata agtctatgtt ttagtctgat tcatgctcaa ggaagcacga atcaagcaaa
      361 cttaattggc cttcaacatc aaattccttg gttttcattt ttattgttct taatcaatgc
      421 gagtatggtt gaagaatttc tgtatcgaga aattttatgg aatttggtca gaaaattaga
      481 tatcagagtt gctttgacaa gtgttttatt tgccttagca catcatacag gaaccattct
      541 agcttggtgt ttgtatgttt cacttgggat gtttttaggt atggtgcgct ataaatctga
      601 cttatggggc agtatgggac tacatttggt gtggaaccta ctagtttata gtttgctgct
      661 tttttaaaat aggtgacctg atttttaggt aacttctaaa actaacttcc agttttggga
      721 gagagatgga agttactttg aaaagttacg gaaacattat tttctatgtg acaacattgt
      781 ataattgctt gcattttact ggtggagaga gtataatgag ggtggataaa gtacttaatc
      841 ccaaaaattt aaataggagg agctagctat ggcagaagaa gtattaaatc ttcaattagt
      901 atctgttcaa gttgacgaaa cagatgaagt tgatggaatg cgatttagta cattcagtac
      961 aaatcgttgc ggtaactgga gtgcttttag ttgggaaaat tgttaattca tttaaattgt
     1021 aaactttaga gattctctgg agttatggat gtcgttccaa tatgtgcacg ttggaatgtt
     1081 agtgcttata tggggagaat ccaaaggatt ctcccttttt atttaaataa gtctgtctag
     1141 ctatagatat attaagtttg ataaaatttc ttggagattt tgttatggat tataatttca
     1201 atttggagca tcctttcttt ttcactaata atgattatag tacagatact agcataaagt
     1261 accaagtaag tctgcctttt aattggcacg aagtaatgaa taatgatgag tgggtttatc
     1321 aataccctat tggaaaattt gttgaaagac aagggtggaa aatacacatt agttcagagt
     1381 ataacagttc acatgaattg ttacaagatg tagcaaaaat ttgccatgaa atgcgaattc
     1441 catttaaaca tcttagtaca gaagacaaat ttatcatgcg aaacgggaaa ttggttagtc
     1501 gaggattttc tggtaaattt attacatgtt atccaaatca aaatgaatta gagtcggttt
     1561 tgcaaaggtt agaaagtgcc ttaaagcaat ataatgggcc atatatttta agcgataaac
     1621 gttgggatga agcaccaata tatctgaggt atggtgtgtt taggccgtca agggatgatg
     1681 aaaagaaggt tgctattgat gaattaattg ttggcgatga agttgttaaa gatgaacgtt
     1741 taccagtatt taaaattcca aaaggtatag tgccccctga ctttttgaat aaatggcttg
     1801 acaagaaaga taaaaaacag ggagactttc cctttattat agataatgca attagatttt
     1861 caaacagtgg tgggatatat aatgctaggc taaaagaaga tgggaaaaaa ataattttaa
     1921 aagaggctag accgtataca ggtctaggat ttgatggtac ttattcttca gagaaattag
     1981 cttcggagtg caaagcacta aaaattctaa atgaatggag tgagacgcct aaaatatact
     2041 ggcacggtaa gatctgggag cacaccttct taggaattga acatatgaag ggcgttccac
     2101 taaatagatg ggttaccaat aattttcctt tatatgaagt tgttgataag accaaagatt
     2161 atctattgag agtttcaaag atagtagaaa aactcatcga tttaacgaat aagtttcatt
     2221 ctgaaaatgt atatcatcaa gatttacatt taggaaatat cttggttaag gatgaagatg
     2281 agattagtat aatagactgg gaacaggctg tcttttcaaa tgatgaaaaa gttgtacata
     2341 aagttgcagc ccctggtttt agagcatggc gagaaacact tcccagtgag atagattggt
     2401 atggtattag acaaatagct cattatttat acatgccact agtaactaca tcggatttga
     2461 catacaacta tgtaagtcaa accaggatag aaggaaaaaa actatttgag tctttgggtt
     2521 atacaagaga acatatagat tatgtagaaa gtttattgtc atatctagat tctaaatgtc
     2581 cacagattga gaatatttcc aggaaaaaag ttttaaaacc catgcatgag attagaacta
     2641 tcgagagtga gcaagatata caagatttta ttataaaatt attgagaggg tttacgctaa
     2701 cttatggaca gtggcgtaaa gaatttcaat caagattctt cccagttcat tattatggtc
     2761 tgaattttaa tcaaggaatt gcgttttcag atttagctat cctttggagt tatcaacaac
     2821 tagctaagaa agtaaaaaat ttcaagtttg atgactatta tgagattcga acacaggtta
     2881 ttaatgaggc agtaaataat tttaagaaat cgtccctttc tggtcttttt gatggtaaaa
     2941 ttggtacaat ctggctcatt tatgaatttg gggagataga tcgggcagtt gaacttttta
     3001 caactcattt tatagagata tttgagaatt cacaaaataa aaatttatat agtgggcagg
     3061 ctggaatttt actggttgga ctgtattttc ttagtaaagg ggaaattgat aataaactag
     3121 gagaagaaat actaattagg cttagagaat atactttaaa ttatatagaa aatccagaaa
     3181 ctttttgtaa agttggcgct agtgatgttc agagtaatga tccttatgag aattttggtg
     3241 gattactata tggtcacgca ggggtagctt ggctcttcgg tgaagcttat aaattaactg
     3301 gtgaaagtat ctataaaaat ggtctagaat tggctgtaga taaagaatta gttgcttata
     3361 aagtagatag taataattca ttgcagtaca gccaaggtca tagattacta ccatatctag
     3421 caactggaag tgctggcctg cttttactta ttaatagaaa taaagaaatc ttgtcaagca
     3481 aatacttaaa atatttaact tctttagaga gggcaactga tgtagttttt tgtgtattac
     3541 caggtttatt taacggattt tgtggtcttg aagttgctaa taatatatat agtgatatcg
     3601 atgataactt ttcaggacaa aagaaattaa ttgaacaact ttatagatat ttgtgtgtaa
     3661 ttgaagaagg atttgttatt gcgggagata atggtttaaa gataacaaca gatattgctt
     3721 cgggatttgc gggtgttgca attggattgg tatcaataat ggataataaa ttgacgattc
     3781 ttcctcaaat ataatattta gataccatta ttaaggggtc atacttgtga atcagagtaa
     3841 tgaaatattt ttaaatacaa tacaggaaaa aacacactat aagaggtcta tctcttcatc
     3901 atttcaggaa ttaaaaaaat ggaattcttt gatatttagg agagggcgat attggtacca
     3961 atttgataat tggagtatac ccaatactgg tgtaataaag gaatggtatg tcgaagataa
     4021 tgactggatt cctaaggtta gttcttccag tttagacaat tcaaattcac ttaaaattgt
     4081 ctttgatgtg gaaaaatata ctttgccaga tgatagtatc ttacaggatg ttaggatttc
     4141 atctgatgag agttatatag ttttggttgt atcctcactg cgtagcacta atttaattgg
     4201 aataaaaaaa tatactatgg aagagttatt tatcattgaa gatatttcag tagaaagttc
     4261 gttctatttg ggtaaattcg gtgttatgta tactcgctcc aaagagtacg ggaggccaag
     4321 taagttattc tataaatctt ttgatagctt tacagaagag gaactgtttg aagaaaacga
     4381 atgctctttt cgattaaaaa tagtccacat tgatagcaat aattgttttg taaagtcagt
     4441 agactttcag aaaggcagga tttttctata cagtttcgac cggacgggtt ttgttagaca
     4501 ctcttacaca gaaacggtag ccccaacacc tagagatatc gctctattta gtacagaaaa
     4561 agcacagtac tttcttgggc tctcttcaac tgaagaaaag aaagatcaac taattctaag
     4621 ggaaacttct tcgggagaca gagtttctat tactatacca tatcaagata gggctagaag
     4681 agttcactgt ataggacgtt atatcctttt agactgttca aacgcagcga attccgtctt
     4741 ttatcttgct acatttaaaa ataatagttt gagggagttg agtgttaata agataacatt
     4801 tgatgaaaag acaacacttt atgaaaattc attttctgat cgtgttcttc tgtttttaga
     4861 gaggagaact ttttacgaaa aaatacttag ttttgattta atagaaaata cattaaaaac
     4921 tgaatttgaa agacccatca taaaatcgaa taatactaaa tatttttcta aggtgatttg
     4981 gactaaaaaa gacagttatg atgtaagcat accgatttct ttattttgga aaagtgaaga
     5041 tacggatgag ctcccaagaa gaaaaaaatg tattctaagt gtctatgggg cttatggtaa
     5101 gaatgataat tctgatttag atgaaattat gttatctata atagatgcag gttttattta
     5161 tgctatagtt catgtaagag gtggaggcta tttgggtggt gaatggtatc gctcaggaaa
     5221 ggcgttaaac aaatggaatt ctatcagaga ttttattgag ggagtaaatt atttaaggga
     5281 aaatgatgta attgacagta agcgattagg tttaataact tctagtgcag gtggaataat
     5341 tgctggtgcg gtgttgaacg aggaaaaaaa tttattgcaa agcattctct tattttcgcc
     5401 atttataaat ccttatgata cacttcagaa tccaaatgat cctctttcta agaccgaaat
     5461 agcagagtgg ggagatatta gagaccctga agtaaaggct tatataaagt catattcgcc
     5521 tatgcaaaat attgaaaagg cacgagactc aaatactgtt atagttaata ttttgggtga
     5581 gaaagaccca tatattaata ataacgaagt gatagagtgg tcaaagaaat taaattctat
     5641 tggagttaaa agtttgttgt atttaaacaa agcagctggt catggaggtt ttactccatc
     5701 agatgttctc ttaatgattg atactttaaa ttatttcttt gaagaagtgg gaaggaataa
     5761 cttatgaatt atttaaaatt tataaaaaag actaaactta tattaatggg gattttaata
     5821 tttctatctt cgtttaatgg tgtcttgctc tcaggaatta ttgtatatgc aggtagttta
     5881 aatcagactt cctcattttc tgacgttcta agatttggtg ctataagtat tctgggttgg
     5941 tcagcaatct atatatcaaa ttactattta gaagtaacgg aagcatcaat aacgaaagat
     6001 ataaatgtaa aaattaaaca aggatatttt agagaacagt atctttcttc tgaaatggtt
     6061 aaagattact catctattat ttcagttttg tcaaatgatt tgagattaat cgaagaaaac
     6121 tattttagac aaatttttga aataatttct tcaatattgt tgttcatcgt ctctctaagt
     6181 tttatgcttt atttaaactt cctagtttca ataatattta ttgtattatc agcattgccg
     6241 attatagtac ctgtctttat gaagaagatg ttgtctaatt cagctaatga gtactctaac
     6301 agcaatgcag agtatactca cataataaaa gaaattttta atggttttaa gacattaaaa
     6361 tcttattctg ttactaaaga aataattagt ttgtcggata aaaagttgga taaactagaa
     6421 gattctactt ttaatttgaa acgatcagag gttctttcaa aattggttgc agtactaatt
     6481 tcaggttttt gctttctagt tcccttggtt gtaggatgtt attttgtaat ttatcataaa
     6541 agcctttctt ttagtgaact gataggtatt ttcttagcaa acgataaagt tttagggccg
     6601 atacaatcaa ttgcctattc attaaataag ataaatacaa ccaaagattt aaggaaaccg
     6661 tttttaaaat acttaagtgg agagaagaat tttatagacg ctgaacatga taataacgga
     6721 ctgtatactt catcaataga tgagatacac atgaaagatg ttgtatattc tattacacca
     6781 gaaaataaat taagtattga cttctcattt aagtcaccat ttagggtatt attaacagga
     6841 acttctggta gtgggaaaac aacgatttta aatttaatta atggttcttt aaagccacaa
     6901 aaaggttatg taaatttgtt atcacatggg aaaaagagtt cagattcaat accaacagtt
     6961 gatcagacac catatatttt tgacactact attcgtgaga acgtaacttt atttcaaaat
     7021 gaatattttt cagatgatca gataattgag gtgttaaaaa aggtaaatct atatgaagaa
     7081 ttagaaaaga tagatatact aaattatcaa tgtggtgaaa atggtagtaa tttgtctgga
     7141 ggtcaaaaac aaaaaatagc tttagctaga gctctgatta gaaataataa agtgtactta
     7201 tttgacgaaa tatcagctaa tttagataat gataattcaa attccataca tgatattctg
     7261 ttcaatttag gtatttcatt tattgaagtt tcacatcatt atgacttaaa tgacaagaga
     7321 tacactgata tatataaatt ggaaaatgga acacttttca aaattaagtg attggaggca
     7381 agaatatgga taaagattat atattaaaag tgaaagggct gtatcatcaa tttttactag
     7441 gaaataataa aacgttgcaa gtgctgaaaa atgtttctct ttctgcttcg agaggagaat
     7501 ttataagtat tctaggaatt agtggttctg gaaagtcaac tttattaaaa tgtatttcta
     7561 gtttgcttga accaacaagt ggggaagtaa ttttaaatgg aatcaacccc tataaaatca
     7621 gaaatgcaaa attgtcaagt ataagacgta acgaagtatc ttttatattt caagcataca
     7681 atttaatacc ttccctgccg gtaatagaaa atatagcact tcctttgcga ttatcacaaa
     7741 aaaaattaac tattaaaaat gtagaaaact tactcaaaag aatgaagttt aatgctggct
     7801 taaacgattt tgttggaact ctgtctggtg gagaacaaca gaaggttgct atagctagag
     7861 cggttattgc tgatagtgat ataatatttg ctgatgagcc aactggggct ttagacagcg
     7921 tttctcgtga agtaattttt gaattattga gagagttagt aggggcgggt aagtgtgtaa
     7981 ttatggtaac gcacgatata gaattggcct cgaaaactga tcgtgcatta atattgaaag
     8041 atggaaaaat tttcaaagaa cttcatagac ctagcgggga agagttgtat aaaatcttag
     8101 aggtacaatc aactacggag gaatagagat gctcagatta gtttactatc aatttttaca
     8161 taataagaaa caatggttag gcgtttcacc agtaattttt gtttcgagtt tagttatggg
     8221 attggctgtt aatggggtaa ttaatgttga gaataattcg caagtatttg tcggacttcc
     8281 agatcctaaa ccaattttta tgtttccgat agtattcgga ggagtaacat tattctttgt
     8341 gctctctaac ataataaata tgttggttga aatttttaga gatgattacg aacttctgga
     8401 ggtgttggga gcaagtctcc ttcaactatc atttcttgtg gggggacaga tttttatcat
     8461 ttcctcgatt attagtttta ttgcatatct atgctctata tttgttacgt caaattatta
     8521 ttatttttta caatactttt tcggggaaaa tatattacct gatatccaat ttcaaacgag
     8581 tgctgttgga tgtatcatta cagtagtgtt gatttcattt ttagcctttc taagtggttg
     8641 cttttatacg ttcaagaaga ttagaaatag gaagagttct aaaataaggc atgttctttc
     8701 aatagtaaaa agaatactgc tattggctgg ttttagtgtt atctggttgc tttctttgca
     8761 acaaattttc caagatagca caattttagc gaaggcccag ataatattca atattgtaat
     8821 cttagatatt gtgattattt atcaattatc tccttttata cagtcatgtt ttatcaaact
     8881 actttctata attatattta gaaataattt tatgttcatt gcttcgaagt ggaatttact
     8941 atatcgtaag ccttatataa aatctatcag tgctgcaatt acaggagcta tattactaat
     9001 ttctagtttt caaatgattt ctcagaatat actatctcaa tttcaagatg acagtgattt
     9061 agagctaaaa gtggcattta ttgtctatgt aggagcacca attttgatag tacttgcaaa
     9121 tattatttca attgcatttt tgtcatcgca tcaagaaagg attgagattc agcaatttga
     9181 aattttagga acttctaatt accagatggt taagattaaa gttggtgagg ctatattttt
     9241 gacatttgtt acttcattaa ttgcattttt acttaacatt aaaattatgg ctttaatata
     9301 ctactcttta gaggatgttt taatagatga tatgaatctt ttggggttaa tacttccaaa
     9361 ttttatagtg tctattattt tatttatttt aatatttatt actaaaagtt cctattttat
     9421 ctttaagaat gcaaaaatta tttcttaaaa atttgtaagt tttcgctata taaaatagtt
     9481 aaatctattt tgtatcattg cttgcatttt actggtggag agaatataat gagggtggat
     9541 aaagtactta atcccaaaaa tttaaatagg aggagctagc tatggcagaa gaagtattaa
     9601 atcttcaatt agtatctgtt caagttgacg aaacagatga agttgatgga atgcgattta
     9661 gtacattcag tacaaaccgt tgcggtaact ggagtgcttt tagttgggaa aattgttaat
     9721 tcatttaaat tgtaaacttt agggattctc tgaagttatg gatgtcgttc caatatgtgt
     9781 acgttggaat gttagtgctt atatgggggg aacagaatcc tctcttgatt gaagacaagt
     9841 tagccattag gctggtttgt ctctttgtca actgtagtgg gttgatataa tagtattagt
     9901 gagtgggata aaagtttcat ttagtttatt cagtacaaat ttaacgggtc aagatttatt
     9961 gaactgcacc ccaatagtta gacagaa
//
LOCUS       DAVBBS010000068         9700 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVBBS010000068 DAVBBS010000000
VERSION     DAVBBS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9700
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.068"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..125
                     /locus_tag="VQU32_001847"
     CDS             <1..125
                     /locus_tag="VQU32_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473252.1"
                     /translation="KASQWFKVSDKWYYVNGSGALAVNTTVDSYRVNANGEWVN"
     gene            complement(355..1335)
                     /gene="dusB"
                     /locus_tag="VQU32_001848"
     CDS             complement(355..1335)
                     /gene="dusB"
                     /locus_tag="VQU32_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET2473253.1"
                     /translation="MTNLNTPFMIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(1322..2194)
                     /gene="hslO"
                     /locus_tag="VQU32_001849"
     CDS             complement(1322..2194)
                     /gene="hslO"
                     /locus_tag="VQU32_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HET2473254.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQERHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGAGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2359..3786
                     /locus_tag="VQU32_001850"
     CDS             2359..3786
                     /locus_tag="VQU32_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2473255.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLDSAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            3945..5453
                     /gene="glpK"
                     /locus_tag="VQU32_001851"
     CDS             3945..5453
                     /gene="glpK"
                     /locus_tag="VQU32_001851"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET2473256.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5495..7321
                     /gene="glpO"
                     /locus_tag="VQU32_001852"
     CDS             5495..7321
                     /gene="glpO"
                     /locus_tag="VQU32_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HET2473257.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPILDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7391..8095
                     /locus_tag="VQU32_001853"
     CDS             7391..8095
                     /locus_tag="VQU32_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET2473258.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            8392..8595
                     /locus_tag="VQU32_001854"
     CDS             8392..8595
                     /locus_tag="VQU32_001854"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473259.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(8682..9269)
                     /locus_tag="VQU32_001855"
     CDS             complement(8682..9269)
                     /locus_tag="VQU32_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473260.1"
                     /translation="MFVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNILSDEVLE
                     VVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVNTIHGNKQ
                     ANTFANFMTGAGISYMIGRIGTLLGGPWGAIIGGAYFGIQAYQSYLDSQSPYPYYITS
                     TYIHVAQKKWKFITEYYRNSNYTGYVKTVTTYVNF"
BASE COUNT         2811 a         1930 c         2193 g         2766 t
ORIGIN      
        1 tgaaagcaag ccaatggttc aaagtatcag ataaatggta ctatgtcaat ggctcaggtg
       61 cccttgcagt caacacaact gtagatagct atagagtcaa tgccaatggt gaatgggtaa
      121 actaaaccta atataactag ttaatactga cttcctgtaa gaactcttta aagtattccc
      181 tacaaatacc atatcctttc agtagataat atacccttgt aggaagttta gataaaaaaa
      241 taactctgta atctctagcc ggatttatag cgctagagac tacggagttt ttttgatgag
      301 gaaagaatgg cggcattcaa gaggctcttt aagagagtta cgggttttaa actattaagc
      361 cttctccaat tgcaagaggg cttcaatctc tgccagggtg ctagcttgcg aaatggctcc
      421 acggagtttg gcagcgccag atgttccacg gagatagtga ggagcgaggc cgcggaattc
      481 acgaactgcg acgttttctc ctttgaggtt aatcaatcgt ttcaagtgtt cgtaggcgat
      541 cttcatcttg tcttcaaagg tcaaatcagg taggatttct cctgtttcaa agtaatggtt
      601 gatttggttg aagaggtaag gatttcccat ggcagctcgg ccaatcatga ctgcgtcagc
      661 accaacttct tcgatgcgtt gcttggcttc ttggacagta cggatatcac cgttggcgat
      721 gaatggaatc ttggttagag cttgggcaac cttgtaaagg gtctcaaggt ctgcgtggcc
      781 agtatacatt tgttcacggg tacggccatg catggcgagg gcagaaacac ctgcagcctc
      841 agcagcgagg gcattttcta ctgcaagaga tgggtccgcc catccggtac gcattttgac
      901 agtaagtggg atatcaagga cagactggac cttgttgatg atagagtaga tcttgtcagg
      961 atccttgagc cacatagctc cagcttcgtt cttcacgatt ttgttgacag ggcagcccat
     1021 gttgatatcg acgatatcgg tcttggtgtt ttcttggatg aattctgctg cgcgtgctag
     1081 gctgtcttca tcgctaccaa aaagttggat agagacaggg ttttcgccct catcgatatg
     1141 aagcatgtgc agggtttttt cgttgttgta ttggattccc ttgtcagaga ccatttccat
     1201 tacaacgagt ccagctccga gctcttttgc gatggtacga aaggctgagt tggtcacgcc
     1261 agccataggc gctaaaacgg tacgattggg aatctcaata ttgccaatca taaaaggtgt
     1321 attaagattt gtcacgaatg agttcctcca ggtccttttc atcaaagttg taagtagttt
     1381 ggcagaattg acaagtgatt tctgccccgt ggtcttcctc tttcatttcc tgtaagtctg
     1441 agcttggaag gctggcaaga gcgttcataa agcgttcatg gctacagtca cattggaaac
     1501 ggatttcttc ttcagaaaga cgcttgtagg cttcgtcccc gtagatagcc ttgaggaggg
     1561 cttcgatatg gtcgtcgctt tcgagaagag tagagatagc tggcatttct tggatgcgtt
     1621 tttcaaagcg agcaatctct tctttcttgg ctcctggcaa gacttgaact aggaaaccac
     1681 ctgcaacctt gaccttgtct tcctcgtcca aaaggacatt gaggccgacc gctgaaggcg
     1741 tttgttggct ttcagtaagg taaaaggcaa ggtcttcacc gatttctcca gagatgaggg
     1801 gagttataga gttgtaagga tttccagcac cgtagtctgt gataacgagg aattgaccat
     1861 ttccaacaaa aggtccgact aggacttcac cagtcgcagt ctttttgatg tcaacaccag
     1921 gattttgaac atagcctttg acgttcccct tggtatcagc gacggtgata atagcaccta
     1981 gagagctaga gcccaacacc ttaactgtaa gtttggtatt tcctttttca ttggctgcga
     2041 gaatctggct agcgataaga gttcgaccaa gcgctacagt tgagctagct tgggtttgat
     2101 gtctttcttg agcagtgcgg acggtttccg tgctatcaag gacaaaagca cgaaaggctc
     2161 cgctttctga tatagtttta ataattttat ccatagctac tattttagca taaaaatgcc
     2221 caaaggggga gccgtgtgtt tactgatttt caggataatg gaccaggaaa tcagcatgaa
     2281 aataaaaaga gaaacagatt atttcagcat ttgtaagatt tatgctatgc ttaaggtaga
     2341 aaatgaaagg ggtaacaaat gtatttagga gatttgatgg agaaagccga gtgtggtcaa
     2401 ttttcaatcc tttcctttct attacaagag tctcagacga ccgtcaaggc tgtaatggaa
     2461 gaaacaggat tttcaaaagc aaccctaacc aaatatgtca ccctgctcaa tgacaaggct
     2521 ttggatagtg gcttagaact gactattcac tcagaagatg aaaatctgcg tctgtctatc
     2581 ggtgcagcta ccaaggggag agatattcgg agcttgtttt tggatagtgc tgttaaatac
     2641 cagattttgg tttatcttct ctaccaccaa cagtttttag cccatcagct ggctcaagaa
     2701 ttggtgatta gcgaggctac gcttggtcgt cacttggctg gtttaaatca gattttgtca
     2761 gaatttgatt tatccatcca aaatggacgc tggagaggtc cagagcatca gattcgctat
     2821 ttctatttct gtcttttccg aaaggtctgg tcgagtcagg aatgggaagg tcacatgcag
     2881 aaaccagaga gaaaacagga gattgccaat ttagaggaaa tctgcggtgc aagtttgtct
     2941 gcggggcaga aattggactt ggttctctgg gctcacatca gtcaacaacg tcttcgggtc
     3001 aatgcttgtc agtttcaagt catagaagag aaaatgcgag ggtattttga caatatcttt
     3061 tatcttcgtt tgctgagaaa ggttccgtcc ttttttgctg ggcaacatat tccactagga
     3121 gttgaggatg gtgagatgat gatattcttc tcttttctcc tatctcatcg cattcttcct
     3181 cttcatacta tggagtatat tcttggtttt ggagggcagt tggcagattt actgacgcaa
     3241 ttgattcaag aaatgaagaa ggaggaacta ttgggggatt atacagagga ccatgtcacc
     3301 tatgaactca gtcagctttg tgctcaagtc tatctctata agggctatat tttacaggat
     3361 cgctacaagt accagttaga gaatcgtcat ccatatttac tgatggaaca tgattttaaa
     3421 gagacagcag aggagatttt tcatgctcta cctgcttttc aacaggggac agatttagat
     3481 aagaagattc tctgggaatg gctccagtta atcgaatata tggctgaaaa cggtggccag
     3541 catatgcgga ttggtctgga tttgacatct ggttttcttg tcttttcaag gatggcagcc
     3601 attttgaaac ggtatttgga atacaatcgt tttattacca ttgaagctta tgaccctagt
     3661 cggcattatg atttgctggt taccaataac ccgattcata agaaggaaca gacaccagtc
     3721 tattatttaa aaaacgactt ggatatggaa gatttggcag ggattcgtca gttattattc
     3781 acttaaaagg cttggttaat ctaggtcttt tttgtgaaat tcacacaatc tcctcacatt
     3841 tttttaaaaa ttaaaaaaag ttgataaaca agaaagcgct ttattttgta tactagtaag
     3901 tgtaaagagg aaacacctca agatctttat caggaggaca gtacatgtca caagaaaaat
     3961 acatcatggc cattgaccag ggaactacaa gttctcgtgc catcattttc aacaaaaaag
     4021 gggaaaaggt tagctcgagt caaaaagagt ttacccagat tttccctcag gcaggttggg
     4081 ttgagcacaa tgccaatgaa atttggaact ctgttcagtc agttattgcg ggtgctttca
     4141 tcgaaagtgg tgtcaagcca aatcaaatcg aggcaatcgg gattaccaac caacgtgaaa
     4201 caacggttgt ctgggataag aaaacaggac ttcctatcta caatgctatc gtttggcagt
     4261 cacgccagac agcacctttg gctgagcaac taaaaagcca aggttatgtg gaaaaattcc
     4321 atgaaaagac tggtttgatt attgatgctt acttctctgc taccaaggtt cgttggattt
     4381 tggatcatgt agaaggtgct caagagcgag cagaaaaagg ggaattgctc tttggtacta
     4441 tcgatacttg gttggtttgg aaattgactg acggtgcggc tcacgtgact gactactcaa
     4501 atgcagctcg taccatgctt tataacatta aagaactcaa atgggatgat gagattttgg
     4561 aaatccttaa cattccgaag gctatacttc cagaagttcg ttctaactcc gaaatctacg
     4621 gcaagacagc tccattccat ttctacggtg gagaggtgcc aatctcaggt atggctgggg
     4681 accaacaagc agccctcttt ggacagttgg cttttgagcc aggtatggtt aagaatactt
     4741 atggaacagg ctctttcatc atcatgaata ctggggaaga gatgcagttg tctgaaaaca
     4801 acctcttgac aaccattggt tacggaatca acggtaaggt ttattatgcc ttggaaggtt
     4861 ctatcttcat cgcaggaagt gctattcagt ggcttcgtga cggtcttcgc atggttgaaa
     4921 attcaccaga atctgaaaaa tacgctcgtg attctcacaa caacgatgaa gtttatgtcg
     4981 ttccagcctt tacaggtcta ggcgctccat actggaacca aaatgctcgt ggttccgtct
     5041 ttggtttgac tcgtggaaca agcaaagaag actttatcaa ggcgactttg caatctattg
     5101 cttatcaagt gcgtgatatc atcgacacca tgcaagtgga tactcagacc gccattcaag
     5161 tactgaaggt ggatggtggt gcagccatga acaacttcct catgcagttc caggcggata
     5221 ttttaggcat tgacattgca cgtgctaaaa acctggaaac aacagctcta ggagcggcct
     5281 tcctagcagg tttgtcagta gggtactgga aagacttgga cgagttgaaa ctcttgaacg
     5341 agacaggaga actctttgag ccatctatga acgaatctcg caaggaacaa ctctacaagg
     5401 gctggaagaa ggctgtgaaa gcaactcaag tctttgcgga agtagacgac taatactggc
     5461 agaataaagc gatttattta gaaagtgtgt aaatatggaa ttttcaaaga aaacacgtga
     5521 attgtcaatt aaaaaaatgc aggaacgcac cctggacctc ttgattatcg gtggaggaat
     5581 cacaggagct ggtgtagcct tgcaggcggc agctagcggt cttgagactg gtttgattga
     5641 aatgcaagac tttgcagaag gaacatctag tcgttcaaca aaattggttc acggaggact
     5701 tcgttacctt aaacaattcg acgtagaagt ggtctcagat acggtttctg aacgtgcagt
     5761 ggttcaacaa atcgctccac acattccaaa accagaccca atgctcttac cagtttacga
     5821 tgaagatgga gcaaccttta gcctcttccg tcttaaagta gccatggact tgtacgacct
     5881 cttggcaggt gttagcaaca caccaactgc gaacaaggtt ttgagcaagg atcaagtctt
     5941 ggaacgccag ccaaacttga agaaggaagg cttggtagga ggtggagtgt atcttgactt
     6001 ccgtaacaac gatgcgcgtc tcgtgattga aaacatcaaa cgtgccaacc aagacggtgc
     6061 cctcattgcc aaccacgtga aggcagaagg cttcctcttt gacgaaagtg gcaagattac
     6121 aggtgttgta gctcgtgatc tcttgacaga ccaagtattt gaaatcaagg cccgtctggt
     6181 tattaataca acaggtcctt ggagtgataa agtacgtaat ttgtctaata agggaacgca
     6241 attctcacaa atgcgcccaa ctaagggagt tcacttggta gtggattcaa gcaaaatcaa
     6301 ggtttcacag ccagtttact ttgacacagg tttgggtgac ggtcgtatgg tctttgttct
     6361 cccacgtgaa aacaagactt actttggtac aactgataca gactacacag gtgatttgga
     6421 gcatccaaaa gtaactcaag aagatgtaga ttatctactt ggcattgtca acaaccgctt
     6481 tccagaatcc aacatcacca ttgatgatat cgaaagcagc tgggcaggtc ttcgtccatt
     6541 gattgcaggg aacagtgcct ctgactataa tggtggaaat aacggtacca tcagtgatga
     6601 aagctttgac aacttgattg cgactgttga atcttatctc tccaaagaaa aaacacgtga
     6661 agatgttgag tctgctgtca gcaagcttga aagtagcaca tctgagaaac atttggatcc
     6721 atctgcagtt tctcgtgggt ctagcttgga ccgtgatgac aatggtcttt tgactcttgc
     6781 tggcggtaaa atcacagact accgtaagat ggctgaagga gctatggagc gcgtggttga
     6841 catcctcaaa gcagaatttg accgtagctt taaattgatc aattctaaaa cttaccctgt
     6901 ttcaggtgga gaattgaacc cagcaaatgt ggattcagaa atcgaagcct ttgcgcaact
     6961 tggagtatca cgtggtttgg atagcaagga agctcactat ctggcaaatc tttacggttc
     7021 aaatgcaccg aaagtctttg cacttgctca cagcttggaa caagcgccag gactcagctt
     7081 ggcagatact ttgtcccttc actatgcaat gcgcaacgag ttggctctta gcccagttga
     7141 cttccttctt cgtcgtacca accatatgct ctttatgcgt gatagcttgg atagcatcgt
     7201 tgagccaatt ttggatgaaa tgggacgatt ctatgactgg acagaagaag aaaaagcaac
     7261 ttaccgtgct gatgtcgaag cagctctcgc taacaacgat ttagcagaat taaaaaatta
     7321 agaaaaaata aaagaggtgg agggcagcat tccttgtcgc ccgtcccttc tttttaatgg
     7381 agacagaaag atgatgaatg aattatttgg agaatttcta gggactttaa tcctgattct
     7441 tctaggaaat ggtgttgttg caggtgtggt tcttcctaaa accaagagca atagctcagg
     7501 ttggattgtg attactatgg gttgggggat tgcagttgcg gttgcagtct ttgtatctgg
     7561 caagctcagt ccagctcatt taaacccagc tgtgaccatc ggtgtggcct taaaaggtgg
     7621 tttgccttgg gcttccgttt tgccttatat cttagcccag ttcgcagggg ccatgctggg
     7681 tcagattttg gtttggttgc aattcaaacc tcactatgag gcagaagaaa atgcaggcaa
     7741 tatcctggca accttcagta ctggaccagc catcaaggat actgtatcaa acttgattag
     7801 cgaaatcctt ggaacctttg ttttggtgtt gacaatcttt gctttgggtc tttacgattt
     7861 tcaggcaggt atcggaacct ttgcagtggg aactttgatt gtcggtatcg gtctatcact
     7921 aggtgggaca acaggttatg ccttgaaccc agctcgtgac cttggacctc gtatcatgca
     7981 cagcatcttg ccaattccaa acaagggaga cggagactgg tcttacgctt ggattcctgt
     8041 tgtaggccct gttatcggag cagccttggc agtgcttgta ttctcacttt tctagtttat
     8101 actcttcgaa aatcaaattc aaaccacgtc agcgtcgcct taccgtactc aagtacagct
     8161 tacggctagc ttcctagttt gctctttgat tttcattgag tattagaaaa caattatgtt
     8221 gatagagctt gggcaagagc ccaatttcag caaaaaatga agtaaatctt ctcataataa
     8281 aacgcatcat atcaagcacg aaaattccac gaggtcaact acagtcagaa agctgaacaa
     8341 caagccaaaa cgcccaaaaa aggcggcaaa aagcaagcac ctgcaagcaa cgtgccgaaa
     8401 tggtcaaatc ctgattatgt caacgaatta gacccaaaaa tcgttgatat gctagtagaa
     8461 tttcacaagt cacaaggcac tttggaaact cccgaggcgc aagcagaaat cgcccaaaaa
     8521 cgtgaagaaa tcgagcaaag gagagctgag cttgaggata aaaaacaaga gcttttgaac
     8581 cgcttgaaca aatagagttt cgcaagtatt atgcttacaa attacttgag caattaacta
     8641 aaatataaac cctgccttta tatctaggca gggtttatat tttagaaatt cacgtaggtt
     8701 gttacggttt ttacataccc agtatagttt gagtttctat agtattcagt gataaacttc
     8761 catttttttt gagcaacatg gatataagta cttgttatgt agtatggata tgggctttgt
     8821 gaatccaagt aagactgata agcttgtata ccaaaatatg ctccaccaat tattgcaccc
     8881 catggacccc ccaataaagt acctatccta ccaatcatat aactgattcc agcaccagtc
     8941 atgaagttag cgaatgtgtt agcttgttta ttcccatgta ttgtgttgac gtaattccaa
     9001 acattaggat cgtatgatct aaaagatata tttaggtcga tttcattctt ttgataagcc
     9061 atataaaatg ccccattgat atagacgccg tcagcacgtc gttcaatagt gtctacactt
     9121 ccatctggat tgacaacctc aagaacttca tcgcttaaaa tatttacttg cgtatctccg
     9181 aaccgcactg atgagccatt ctcaaactga gcctcaccag atacaacttt agagtttgcc
     9241 gataagctat catcagcaaa aacaaacaag cgacggggaa atgctagaca tacagaaaac
     9301 agacataact agcaaacaca tgcatttaaa catcttagac ataacggaaa ctcctttgta
     9361 tttttgattt ttttcaactt ttattataca ataaaaccaa ataaaaagaa agcggtaaca
     9421 atatgcttaa tgcgaaaatt ttttatatat ttttatgttt gatcgttatc gaaactacag
     9481 gcttgttgtt gttgaaaaga ggtctcgaaa tgggttattt agacacagaa gctattatcc
     9541 tcgcagtttt ttcatttgct ttttacaacc tatgttcatt cgcttgggtc tgctctacaa
     9601 taaaaaacaa taaaaaataa atagacgtat tttcaaaaaa aaaaaaaaaa aatgcatatt
     9661 tatcttagca aaacgacaat ttaaatcgtc gtttttttgt
//
LOCUS       DAVBBS010000069         9471 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVBBS010000069 DAVBBS010000000
VERSION     DAVBBS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9471
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.069"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            150..686
                     /locus_tag="VQU32_001856"
     CDS             150..686
                     /locus_tag="VQU32_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HET2473261.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALAVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            737..823
                     /locus_tag="VQU32_001857"
                     /pseudo
     CDS             737..823
                     /locus_tag="VQU32_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(892..1107)
                     /locus_tag="VQU32_001858"
     CDS             complement(892..1107)
                     /locus_tag="VQU32_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HET2473262.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            1205..1525
                     /locus_tag="VQU32_001859"
     CDS             1205..1525
                     /locus_tag="VQU32_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473263.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(1556..1894)
                     /locus_tag="VQU32_001860"
     CDS             complement(1556..1894)
                     /locus_tag="VQU32_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473264.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(1906..2625)
                     /gene="trmD"
                     /locus_tag="VQU32_001861"
     CDS             complement(1906..2625)
                     /gene="trmD"
                     /locus_tag="VQU32_001861"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET2473265.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(2615..3133)
                     /gene="rimM"
                     /locus_tag="VQU32_001862"
     CDS             complement(2615..3133)
                     /gene="rimM"
                     /locus_tag="VQU32_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET2473266.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKRYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(3164..3952)
                     /locus_tag="VQU32_001863"
     CDS             complement(3164..3952)
                     /locus_tag="VQU32_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2473267.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            complement(4529..4768)
                     /locus_tag="VQU32_001864"
     CDS             complement(4529..4768)
                     /locus_tag="VQU32_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET2473268.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(4788..5060)
                     /gene="rpsP"
                     /locus_tag="VQU32_001865"
     CDS             complement(4788..5060)
                     /gene="rpsP"
                     /locus_tag="VQU32_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET2473269.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            5230..5439
                     /locus_tag="VQU32_001866"
     CDS             5230..5439
                     /locus_tag="VQU32_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473270.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            5918..6139
                     /locus_tag="VQU32_001867"
     CDS             5918..6139
                     /locus_tag="VQU32_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473271.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            6222..7025
                     /locus_tag="VQU32_001868"
     CDS             6222..7025
                     /locus_tag="VQU32_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET2473272.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQSSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(7081..8622)
                     /locus_tag="VQU32_001869"
     CDS             complement(7081..8622)
                     /locus_tag="VQU32_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2473273.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMEMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DLPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(8624..9106)
                     /locus_tag="VQU32_001870"
     CDS             complement(8624..9106)
                     /locus_tag="VQU32_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET2473274.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVYFCFTPQEQSTVGVGTPDIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            complement(9106..>9471)
                     /locus_tag="VQU32_001871"
     CDS             complement(9106..>9471)
                     /locus_tag="VQU32_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HET2473275.1"
                     /translation="KLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAELLKNI
                     KKSSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGTDIDAACGQ
                     LLFNTMKRDRQKAVAAVNP"
BASE COUNT         2796 a         2028 c         1698 g         2949 t
ORIGIN      
        1 attttcgaat caacaaaaaa attatccaac ttgactagtc acgaaaatcg gctgttacag
       61 tttttgttac cttgttttta aaaaatggtt gacatcgact cctatatgag tataatagtt
      121 atcatacatt agaaaagagg ttaactattt tgaaaaaagc tcacgtttat gctatccctg
      181 ctattggggc tgctctcatt gctgctttgg cacaaatcag tcttccaatt ggacctgttc
      241 ccttcactct gcaaaacttt gcaatcggct tgattgctac tgtctttaga ccgagagagg
      301 ctgtactttc tgctggactc tatcttcttc taggtgctat cggtcttcct gtctttgcag
      361 gaggtggagc tggttttcag gctttagttg gccctactgc aggctatctt tggttttatc
      421 tcgtttactc tggacttact tcctctctaa ccaacagcaa gagtggtgtt gttaagattt
      481 ttcttgcaaa cctcttgggt gatgcccttg tctttgtcgg cgggattctc agcttgcatt
      541 tcctagctgg aatggcattt gaaaaagctc ttgctgtggg ggttcttccc tttatcattc
      601 cagaccttgg caaacttctg gctattagtt ttattagccg tcccctactt caacgcctta
      661 aaaatcaggc ttactttact aactaaaaaa ggatatcgag ttatcatgac tcaatatcct
      721 tttcttttat tttgaaaact tatactcaat gaaaatcaaa gagcaaacta gaaagctagc
      781 cgcaggctgc tcaaaacact gttttgaggt tgtggatgaa actgacgaag tcagtaacca
      841 tacatacggc aaggcaaagc tgacgcggtt tgaagagatt ttcgaagagt attagttgcc
      901 tttaaaggca tccaccatcg tttgaaattc ttcatttgag agagtaatcc ctttgcccat
      961 tttagtatgg tctggactcc aagcacgaat atcaaacttt gcaggggcac cattaaagct
     1021 cacacggtta atttccttgg tccaaccttt ttcgttttca gaaagagtca acaagtgctc
     1081 ttcgatttca aatgtaaatt ctgccatttt cttctccttt tttagtttca ttagtttatt
     1141 cgtaaaatct tgtagatttt aggaaaattt tatataatat tgatataaaa gaagggaggc
     1201 caatatgaga cataaattcc agcaagttct aaataaaata catgattttt taaatggata
     1261 tgaccaacct gaccagactg aaaccaactc ccttacagcc actattgaag aggctatcca
     1321 gaaacaaacc gctgttcacc ttatcttgtc tgagacaagt tttacaggtg atatcatcaa
     1381 atatgatcag caaggccagc aaattatcgt gaaaaatttt gccaaaaatg tgagccggat
     1441 tatccgtata agcgatattc aacgcctgcg atttgtcccc tcaactgtcc aaacagccca
     1501 aaaaaataga tttaagaaag agtgagatgt agttgcttca tcccactctt ttttcttagc
     1561 gaatttgttc aaaatgtaaa tgaactgcaa tatgatctcc ataaccactt ctttccaagt
     1621 cacgttgtaa acgataggaa atgtagtgtt ctgcaatggt aatgtaacct gcgcccaata
     1681 aacgatgttc aaccatagat tgaatcatac tgatagtcgc acgttccacc ttggcttctt
     1741 gtaaatccaa aactaccttc ttagtgactt gagcaagatt ttgacgcaaa tcatctgtca
     1801 aaacataaac agtttgggct gccttcaaga tggcttggta aatcttatct ggattaaatt
     1861 cagcaatttc gccattacgt ttgattactt gcataggttt ctcctttatt ctttgttttc
     1921 tttgatttct gccagcattt tttcttcttc tactgtcagt tgataatgtt caagtaaatc
     1981 cggtctgcgc tcgtaggttt tctttaaact ctcgtacaat cgccactgac gaatcttttc
     2041 atggtggcca ctcatcaata catctggcac gaccatgcct cgataatcat agggacgtgt
     2101 gtactgagga tattctaaaa gacctgaaga aaaactatca tcttggtggc tagactcctt
     2161 gccaatcact tctggaatca ggcgaactgt agcatcaatc atggtcatag ctgccaattc
     2221 tccaccagtg aggacatagt cgcctaggga aatctcatct gttaccaagg tcttaatgcg
     2281 ctcatcataa ccctcatagt gcccacagat aaagattagc tcttcctctt gagccaaatc
     2341 ttcagcataa gcctgatcaa actgctttcc agcaggatcg aggagaataa cgcgcggatt
     2401 tttcttttca atagcatcaa aggaatcgaa aataggttgt gctctgagca acatgccctg
     2461 accgcctccg tagggctcat catctacatg acgggccttt tcagcatttt ctcgaaaatt
     2521 atgatactgg atatccaaga gccctttttc tcgagccttt ccaacgattg agtgctccag
     2581 tggagaaaac atctctggaa agagggttaa aatatcaatc ttcatcgtct aacccttcta
     2641 agatttccac atcgacccgt ttatttggaa tatcaacatt gagaaccact ggtgggatat
     2701 aaggtaaaag caaatcacgt ttgccttttc gtttgaccac ccagacatca ttagcacctg
     2761 gttgcaggat ttccttgatg gttccaacca agctatcacc ctcatagact tccaaaccga
     2821 taatctcgtg atagtaaaat tcaccatcgt ctaggtcatt caaatcttcc tcagcgacct
     2881 tgagactata tctcttgtac ttttcgatag tattgatatg gtacatatct ttgaatttaa
     2941 taatgtcaaa gttcttctgt ttacggtggc tagcgatggt cactgtttgg acaaactgat
     3001 ctttttcatc aaacaaagcc agctcagctc cttttttaaa ccgttcttct gcaaaatccg
     3061 tcacagacaa gactcgcatc tcaccctgta atccctgcgt attaacgatt ttcccaacat
     3121 taaagtagtt catcttgtct cctgtaatct ccttttttcc atcttattct gacaattctc
     3181 gaataatagc cgcaattttt tccgattctg accattgtaa ataatggtga ttccctccta
     3241 aaatgagttt agtattggaa gtccaatatt ctgattctct gtactctttt tctctataag
     3301 gctgacaaaa aacaaataca ggaatatgag cttctataga tacatcctca aaatcttcct
     3361 cagtaatctc tccagatatc tgaaattctg gatcttgatt ttccaactct aagccttttt
     3421 cttgcattaa ttcccagatt tttttattcg tttcaggact aaatgttgct tgagttaagt
     3481 tcttaaaata aagttcagga ccacactcgt caatcagcct catctgctct tccatttctg
     3541 gataaggatt ttctgaaaaa tcagcaaaca tgactttttt agttgtcggt tcaattgcta
     3601 ctaaagtctg acgcttaatt ggtttctcga gtaatttgca agctaaaatt ccactccaac
     3661 tatgtgcaca aagtatatat tcagaaattc ctaattcttc aagtacttca taaaccgcat
     3721 ctgcaagatt atctagattt tttccagctt ggtcatgaat cggactccta cctgtgttcg
     3781 gaaaatcaat tgtcaaataa ccaattgtag gaggaggttt ttcaagtata agtgaaaaat
     3841 tttcataact tggtagcaaa cctgctccgt ttagacaaac tagcactttc ttttgctttt
     3901 gataagtaac agagaggcta ccaatttctg tagatacttc aaacctcttc ataaagaaat
     3961 ccactcattc tatataatga attattaaaa atccttatct tttattttat cacgttccaa
     4021 ggattttctc aagttggagg aaggggacaa catctctact ttcccttcaa aaatccttcc
     4081 aaatcatgtt tatgttggta attaacggct gcggttttgt ctttctcaaa gacagtcttg
     4141 gtaaggtcaa tatgattaat agctacgatt gcgacggtgt agtaaatgat atcagccagt
     4201 tctctggcaa gttcctcgtt cgaatcctat cccttctttt cgaccagagc gcctattcaa
     4261 aacctcggct acttctccga cttcctccac taacttcata aagagacctt catcagtccg
     4321 agactgctgt taatgttcga ttaagtagtc ttggaattgc ctaaacgttc aatcttttat
     4381 agtatattga aactagaata gtacaccttt acttctaaaa cattgttaga aatcgatttg
     4441 actgtcctga tcgatttgtc ctgttcttgt ttcattttac tatatcttct attccacaca
     4501 aaaaagcgag acatccgtcc cgcccttctt atttttcgtc aataacgatt cttacttttt
     4561 tgtattcagt tgggacagag tagacaatcg ttcttatcgc agaaatagtg cgacccttac
     4621 gaccgattac acgacccaca tcgctttgat caagattcaa atgatattcc aaaaattctg
     4681 gtgtatcctc aatcttgata gttaaggcat ctggttgtga aattaagggt ttcacaatcg
     4741 caataatgag attttcaatc gtatccatct gtcaacctac tttaaactta ttttgaaaat
     4801 ttagaatcgt ggaatttttt caatacgcct tcttttgaaa ggatgttacg tactgtgtct
     4861 gaaggttgag ctccattagc caaccatgca agaacgcggt cttctttcaa agttacttgg
     4921 ttttcagcaa caagtgggtt gtaagttcca actgtttcga tgaaacgtcc gtcacgtggt
     4981 gaacgtgaat ctgctacgtt gatacggtag aaaggttttt tcttagaacc catacgagtc
     5041 aaacggattt taactgccat ttttaaagtc tcatttcttt aattttttat ttcggtgaaa
     5101 tagctgagct atttagcaca tgttctatta tagcagattt ctggcatgtg tcaagaaaaa
     5161 aacttgacag actataaatt tcttaaagta tttagaaatt tgttagaata ggaaataaat
     5221 gtttgaaaga tgctatccgt gaatactatt ttagaaaaat tttataaaga acaccaagtc
     5281 aaacccttca tatcgcctga acgagagttg gatacttggc ttctaagtcc caagcccgtt
     5341 cccaagcgaa atatggactt attagtagat gattcactag caggagatat cattttacta
     5401 tggagaatcc agtttggtac ttttaccact gaaacatgat tttaaaaact cccattcgaa
     5461 tatgattcaa aattcgctta gaatcaatgt tttcccgctt tttcaaaaaa taaattttca
     5521 tctaatttaa ataactttca attaagtgtg aattttgctt aaaatcatta aaaagcaccc
     5581 catggtgtga accatgaagt gttgtaaatg ctgtgttgta gctagttctt agcgtttaga
     5641 gaactggcta gctttacgag ctttcttaag atctggtttc caaaatactt tgagtacact
     5701 tatggtattg atgcccctaa acatctaaaa actctggtag aaaaagggta tgctcttgtc
     5761 gaaacttctt ttgactcact tgaccatcta aataatacca tgaaaaagaa tatcctgaag
     5821 aagaagggag ttactggact ttctaaaatg aaatcttgcg acctggatca agcccttcat
     5881 gagcattttt cagaagaaga attagctggt cactttcatg tccttctatg gactttttta
     5941 caatggcatt gctatcacac ccaataccta tctaagcgcc tggttcgtaa actttattgc
     6001 agctcttcct ctaaatttcc taattgttga accaattgcc cgttttatac taagttcttt
     6061 tcagaaacca tttactgggg aagaagttga agaagttgaa gattttcaag atgatgatga
     6121 aatcccaact attatctaag ccagttctgt aaactactaa tatttgaaat ccacttcttt
     6181 ttagggtaca atggtataaa tgaatttttg agaggatcag aatgaaaaaa ctagcaaccc
     6241 ttcttttact gtctactgta gccctagctg ggtgtagcag cgtccaacgc agtctgcgtg
     6301 gtgatgatta tgttgattcc agtcttgctg ctgaagaaag ttccaaagta gctgcccaat
     6361 ctgccaagga gttaaacgat gctttaacaa acgaaaacgc caatttccca caactatcta
     6421 aggaagttgc tgaagatgaa gccgaagtga ttctccacac aagccaaggt gatattcgca
     6481 ttaaactctt ccctaaactc gctcctctag cggttgaaaa tttcctcact cacgccaaag
     6541 aaggctacta taacggtatt accttccacc gtgtcatcga tggctttatg gtccaaactg
     6601 gagatccaaa aggggacggt acaggtggtc agtccatctg gcatgacaag gataagacta
     6661 aagacaaagg aactggtttc aagaacgaga ttactcctta tttgtataac atccgtggtg
     6721 ctcttgctat ggctaatact ggtcaaccaa acaccaatgg cagccagttc ttcatcaacc
     6781 aaagctctac agatacctct tctaaactcc ctacaagcaa gtatccacag aaaattatcg
     6841 aagcctacaa agaaggtgga aaccctagtc tagatggcaa acacccagtc tttggtcaag
     6901 tgattgacgg tatggatgtt gtggataaga ttgctaaggc cgaaaaagat gaaaaagaca
     6961 agccaactac tgctatcaca atcgacagca tcgaagtggt gaaagactac gattttaaat
     7021 cttaaaaacc aaaaaaatac agtatccaca ttcggtactg tatttctttt actctcattc
     7081 ttaagttaaa ttattaaaat cccatatttg gtctatccag ccttcataaa agtctggctc
     7141 gtggcagacc ataaggatag atcccctata ttctttgaga gcgcgtttga gctcatcctt
     7201 tgcatccaca tccaaatggt tggtcggctc gtccagcact aaaacgttgt tttcacgatt
     7261 catcaagaga cagaaacgaa ccttggcttg ctctccccct gataatactt gaatctggct
     7321 ttcaatatgt ttggttgtca aaccacaacg ggcaagggct gcacggactt ctgcttgatt
     7381 aagggcagga aaggcattcc agacagcttc aagaggagtt tggcgattac cgccttctac
     7441 ttcctgctca aaataaccaa gttctaaata atctccacgc tccacttccc cagcgattgg
     7501 cgagataatg cccaagagac tcttcaagag agttgttttt ccaataccat tagcaccaat
     7561 aatcgcaacc ttttgattgc gttcgaaggt aagatttaaa ggcttagtaa gaggaaggtc
     7621 gtaaccaatt tgcaagttct tggcttggaa gataaagcgc cctggtgtac gagctggttt
     7681 gaaatcaaag gatggttttg gtttctcact ttggagttcg ataatatcca tcttatccaa
     7741 tttcttttga cgagacatag ccatattacg agttgcaaca cgggctttat tacgagccac
     7801 aaagtccttg aggtctgcaa tctctttctg ctggcgttcg taggctgcct ctagctgaga
     7861 tttcttcata gcataaactt cttggaactg gtagtagtca ccagagtaac gcgtcagctg
     7921 ttgattttcc acatgataga caatattgat aacgtcattg aggaatggaa tatcgtgcga
     7981 aatgagaaca aaggcattct catagttttg gagatagcgc ttgagccaat caatatgctc
     8041 agcatccaag tagttggtcg gctcgtccaa cagcaagata tcaggctttt caaggagaag
     8101 ttttgccaaa agcaccttgg ttctttgccc acctgacaaa gaagttacat ccgtatccat
     8161 gccaaagtcc ataacaccaa gagcacgcgc tacttcgtca atcttagcat ccaaggtata
     8221 gaaatcacga ctctccagac ggtcttgaag ttctcctact tcttccatga gagcatcaac
     8281 atccgcaccg tcttcagcca tttccatata gaggtcattg atacgagctt cagctttgaa
     8341 aagctcatca aaagccgtac ggagaacatc acgcaccgac tgtctttcag caaggacaga
     8401 gtgctgatcc aagtaaccag ccgtcacata tttggaccac tcaacctttc cttcatctgg
     8461 cagcatttta ccagtcacga tactcataaa ggttgatttt ccttcaccat tggcaccgac
     8521 caggccgata tgttctccct tgaggagacg gaaggacaca tcttcaaaaa ttgcacggtc
     8581 accaaaaccg tgactcagat ttttaacttc taaaatactc attttaattc cttatcttgt
     8641 ttttatgtaa tcgtttataa aggagccaag ccagatagcc acccaaagtg ttggtccaca
     8701 aatcatcaat ctcaaagacg cgattgaaat caaagaaaaa gtccaagatt aattgcgtac
     8761 actcgattcc aagactcaca agaaaactaa aaagaaggac cttttttgtt ttccgcaaat
     8821 ttggaaatag ataaaggagt tggaaaatca gaggaaaaaa caagaagaca ttgaggatat
     8881 tttgtaaaaa aatccaacat aattgtccaa tgtcactcac ttcgcccagt ttccagagag
     8941 aattgaaagg agtcaaaaga aaaaccaggc gtccaagatg ctgaatatct ggagttccca
     9001 ctcccacggt agattgttct tgaggagtaa agcaaaaata gacaatgcaa atgctataga
     9061 aaatgactcc ccagaccaaa atatgattat aagtcttctt catcattaag gatttaccgc
     9121 tgcgactgcc ttctggcggt cacgtttcat tgtgttaaag agcaattgtc cacaagctgc
     9181 gtcaatatct gtaccatgct cttgacgaac cacacagttg cccccttttt tcttaagcgt
     9241 atcatagaaa gccaacacgc gctctttggg actacggcta tattggtcat gctcactaac
     9301 tgggttataa ggaatcaagt ttacataaga cgatttcttg atgttcttga gcaattcagc
     9361 caattccaag gcttgttcca caccgtcgtt gacttcatta agcatgatat attcaaaggt
     9421 tacacgacgg tttgttgtct caatgtagta ttcaatagca gcaaagagtt t
//
LOCUS       DAVBBS010000070         9136 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVBBS010000070 DAVBBS010000000
VERSION     DAVBBS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9136
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.070"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            1..294
                     /locus_tag="VQU32_001872"
                     /pseudo
     CDS             1..294
                     /locus_tag="VQU32_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            370..819
                     /locus_tag="VQU32_001873"
     CDS             370..819
                     /locus_tag="VQU32_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2473276.1"
                     /translation="MCTILVSIYYKIGLSSSGVYKAVADGEMTVSLSYEDPAVKLLND
                     GANIKVVYPKEGTVFLPASAAIVKKSKNMENAKKFIDFIISQEVQDTLGTTTTNRPVR
                     KNAKTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDIQSKQ"
     gene            832..1491
                     /locus_tag="VQU32_001874"
     CDS             832..1491
                     /locus_tag="VQU32_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2473277.1"
                     /translation="MSEIEIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIEGGEFYFDDTKINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEP
                     LSNLEAKLRLDMRQAIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     K"
     gene            1618..1923
                     /locus_tag="VQU32_001875"
                     /pseudo
     CDS             1618..1923
                     /locus_tag="VQU32_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809465.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
     gene            1913..3558
                     /locus_tag="VQU32_001876"
                     /pseudo
     CDS             1913..3558
                     /locus_tag="VQU32_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(3661..4353)
                     /locus_tag="VQU32_001877"
     CDS             complement(3661..4353)
                     /locus_tag="VQU32_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2473278.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGCEKMEEGSI"
     gene            complement(4597..5934)
                     /locus_tag="VQU32_001878"
     CDS             complement(4597..5934)
                     /locus_tag="VQU32_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HET2473279.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALTETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGAAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     IGDSVARVLEEMGLETVGTHGTTVALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            complement(5944..6210)
                     /locus_tag="VQU32_001879"
     CDS             complement(5944..6210)
                     /locus_tag="VQU32_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET2473280.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            complement(6475..6861)
                     /gene="rplQ"
                     /locus_tag="VQU32_001880"
     CDS             complement(6475..6861)
                     /gene="rplQ"
                     /locus_tag="VQU32_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET2473281.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(6873..7808)
                     /locus_tag="VQU32_001881"
     CDS             complement(6873..7808)
                     /locus_tag="VQU32_001881"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET2473282.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            complement(7851..8234)
                     /gene="rpsK"
                     /locus_tag="VQU32_001882"
     CDS             complement(7851..8234)
                     /gene="rpsK"
                     /locus_tag="VQU32_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET2473283.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(8252..8617)
                     /gene="rpsM"
                     /locus_tag="VQU32_001883"
     CDS             complement(8252..8617)
                     /gene="rpsM"
                     /locus_tag="VQU32_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET2473284.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(8635..8751)
                     /gene="rpmJ"
                     /locus_tag="VQU32_001884"
     CDS             complement(8635..8751)
                     /gene="rpmJ"
                     /locus_tag="VQU32_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET2473285.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            complement(8776..8994)
                     /gene="infA"
                     /locus_tag="VQU32_001885"
     CDS             complement(8776..8994)
                     /gene="infA"
                     /locus_tag="VQU32_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET2473286.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
BASE COUNT         2766 a         2044 c         1567 g         2759 t
ORIGIN      
        1 tcaaaagaaa atgataatgt tatcaaagaa tatcaaaaca caactggctt ctctactcct
       61 tatacactag atggtagtgt tttaatcgtc caccctgatt taactaaagg catgaacatc
      121 gaaggatata gcgaccttct caaacctgaa ctaaaaggaa aaatcgcaac tgctgaccca
      181 gcaaactctt ctagcgcctt tgctcaatta acaaatatgc tacaagctca aggtggttac
      241 aaagatgata aggcttggtc ttatgtaaaa gatcttttca cacttattga tggtatagta
      301 aaatgaaata agaacaggac aaattaatca ggacagtcaa atcgatttct aacaatgttt
      361 tagaagtaga tgtgtactat tctagtttca atatactata aaatcggttt aagttcatct
      421 ggtgtctata aagcagtcgc tgatggagaa atgactgtta gtctctctta tgaagatcca
      481 gcagttaaac tcttaaatga cggagctaac attaaggtag tctatccaaa agaaggaacc
      541 gtcttcctac ctgctagtgc tgctatcgtt aaaaaatcta aaaatatgga aaatgccaag
      601 aaatttatcg attttattat ctctcaagaa gtacaagata cacttggtac aaccactact
      661 aaccgtcctg ttcgtaaaaa tgctaaaaca agcgaaaaca tgaaaccaat tgacaaaatc
      721 aaaacactca ctgaagatta tgattatgtc atcaagaata aatcagatat cgttaagaaa
      781 tacaacgaag tctttacaga tatccaatct aaacagtaaa agaggttcac tatgagtgag
      841 atcgaaatta ttaacgccaa aaaaatctac cacgatgtcc ctgttattga gaatttgaac
      901 attacaattc caaaaggaag tctctttacc cttcttggag cttcaggatg tgggaaaacg
      961 acccttcttc gtatgattgc aggtttcaac agtatcgaag gtggagaatt ttacttcgat
     1021 gatacaaaaa tcaataatat ggaacccagc aaacgcaata tcgggatggt tttccaaaac
     1081 tacgctattt tcccacattt gactgtccga gacaacgttg cttttggtct tatgcaaaag
     1141 aaggttccaa aagaagaatt gattcaacag accaacaagt atcttgaact catgcaaatt
     1201 gctcaatatg cggatcgaaa gcccgataaa ctcagtggtg gacaacaaca acgtgtcacc
     1261 ttggcatgcg ccttagcggt taatccaagt gttctcctca tggacgagcc acttagtaat
     1321 ctggaggcca aacttcgctt ggatatgcgt caagccatcc gagaaatcca acacgaagtg
     1381 ggaattacaa ctgtttatgt aacccacgac caagaagaag ccatggctat ttcagaccaa
     1441 attgctgtta tgaaagatgg ggtgatccaa caaatcggcc gaccaaaata actctatcat
     1501 aaaccagcta atgagtttgt ggcaaccttt atcggacgca caaatattat ccctgccaat
     1561 cttgaaaaac ggagcgacgg cgcttatatc gtcttttcag atggctatgc ccttcgaatg
     1621 ccagctcttg atcaggttga ggagcaagct attcatgtaa gcattcgtcc cgaagagttt
     1681 atcaaagatg aatctggaga tatcgaagga actattagcg atagcgtcta tcttggacta
     1741 aatacggagt atttcattga gacaggtttt gcctcaaaaa tttaagttag tgaagaatca
     1801 acttttgaag aagaactaca aaaaggcaat cgtattcgtc tacgaatcaa tacgcaaaaa
     1861 ttaaacatct tttctgcaga tggttcccaa aacctgataa aaggagtcaa ccatggaatg
     1921 taaaaaacta aatatttgga cagcttcctc tttcttcatc tttcttacct atcttgtctt
     1981 tctcgtttat cctatcgtta ccgtgctcaa gcaagcactt atacatgaag gacaattctc
     2041 actagctaat tttgtcactt tctttagtaa agcctactac tctgagacac tagtcaacag
     2101 tttcaaggtt tccattaccg ctactatcac ttccttagtt gtaggaaccc tattagctta
     2161 tctcttctct atgtatgact tcaaggggaa gaaatttcta caaatattga ttatcattgc
     2221 ttccatgtca gctcctttcg taggagccta ctcctgaatt ctcttgctgg gacgaaatga
     2281 ggtcatcact aaatttttga caaatgccct tcatcttcca gctatcgata tttatggatt
     2341 caaaggaatt gtacttgtct ttacactgca actattccca ctggtatttc tatatgttgc
     2401 tggaacaatg aacagtattg acaattctct acttgaagca gctgaaagca tggggtcctt
     2461 cggatttaag cgtatcgtaa cggttgtttt acctctccta gttccaacct tactagcagc
     2521 tccttgcttg tatttatgag agcattctca gactttggaa cgcctatgtt gattgacgaa
     2581 ggatatcgga ctttccctgt cctgatttat acccaattta ttagcgaggt tggaggaaat
     2641 tctgcttttg caattatggc gattatcatt gccttggcaa ttttccttat ccaaaaacac
     2701 attgcaaacc gctacagttt cagcatgaat ctgctccatc caattgagcc taaaaaaact
     2761 acaaaaggaa aaatagctgc catttatgca acagtctacg gaattatctt tatctctgtt
     2821 ttacctcaaa tctacttaat ttatacctct ttcctaaaaa catcaggtat ggtatctgtt
     2881 aaaggttatt ctccaaacag ttacaaggta gctttcaatc gtatgggatc tgctattttc
     2941 aataccattc gtatcccttt gattgcctta gttctagttg ttctatttgc gacatttatc
     3001 tcctacctag ccgttagaaa acggaatttg tttacaaact taattgacag cctcagtatg
     3061 gtaccttata ttgtaccagg aaccgttcta gggattgcct tcatttcttc cttcaatact
     3121 ggtctatttg gaagtggatt tcttatgatt acagggactg ctttcatctt gattatgtct
     3181 ctatctgtca gaagattacc ttatactatt cgctcatctg ttgctagctt acaacaaata
     3241 gcaccaagta ttgaagaagc tgctgaaagc ttaggaagta gtcgtctcaa tacctttgct
     3301 aagattacaa ctccaatgat gctatctggt atcatttctg gagccatctt atcttgggtc
     3361 acaatgattt caaaactctc tacttctatc ctcctctaca atgtcaaaac aagaacaatg
     3421 actgtagcta tttatacaga ggttctcaga ggaaattacg gtgtagccgc agccttgtca
     3481 actatcctga ctgttctaac agtaggttcc ttgctcttgt ttatgaaaat ctctaaaagc
     3541 aatagcatta cactttagtt ttccacatca aaaacagccg aaaggagtgc cctcggctgt
     3601 tttttcttat cttgatattc ttatcaaatc cctagctcat ggcaagcaag tctagactga
     3661 ttaaatagag ccttcttcca tcttctcaca tcctagctct cggtaactac ggtcaccgac
     3721 aacttcaacc ctaaactgac cgtcctcata ttcaagaatc gtcacgctac cattatctag
     3781 accatgcgga tgcatgccat taatcagata aacaatggtt ccaatagtca ttccatggct
     3841 gacaacaagg gcgttacctc caccttgatc ttccatttct tttgcaatca tttcaaagcc
     3901 ttccttgatt cggccactga gtttttccca gccttcagcc caaccagctg tatcgacctc
     3961 taccaagccc tcagccagtt cagcataaga caattggtga acgtggtcca cattaaagat
     4021 acgaggaata atgcccatga aaagatcgcc atcataggct ccatcaaaac taccgaaaca
     4081 ccattctctg atacgcttgt ccatgcgata agggattttc ccctgcaagc caagttcttc
     4141 aaggataatt cccatggtct gaatggtacg accagaatca ctcgaataag cgcgctcaaa
     4201 ctgtagatca gattctcgca aaccgattcc taactcttga atccctcgtt caccttcagc
     4261 agttaaggga gtatcgctcc aaccttgcgc gcgaccaatc gtgttaaaca tggtcttgcc
     4321 atgacgtacc aaatacaatc gtacttttac cattttccgt cctccgtttg cttctattat
     4381 atcatgaatg ataaaataaa agccacccat acaggcgact tttgaaggag attattatga
     4441 aaaagtttag gagttcatta aataaagata ttagatgaaa atcaaattca aactaattca
     4501 gtgttatctg tttcaaataa tattaggagg tctttattta atgattatag tatacacatc
     4561 taatcttaaa tagaacttaa aatttctcct attttcttaa tttttaaaac tatgaattgg
     4621 tgctgggatt tgtccaccgc gagagatgaa gtcgacagac gaagccccat taaccttcat
     4681 aacaggtgca gttcctaata gaccaccaaa ctcaatcata tcgccttctc ttcctttggg
     4741 aatgatacga acagctgttg ttttcatgtt gataacaccg attgctgctt catccgcaat
     4801 catagccgca atggtttcag caggcgtatc ttctgggatg gcaatcatat ccaatccaac
     4861 agaacagata gccgtcatag cttctagttt ttctaaatta agagagccat tttgcactgc
     4921 agcaatcatt ccttcatcct cagaaacagg gataaaggcg ccagataaac caccgacttg
     4981 gttgcaggcc atcactccac cctttttaac ttggtcgttc aagagggcca aggcaaccgt
     5041 cgttccatgc gtgccaactg tttctagccc catttcctca aggacacgtg ccacagagtc
     5101 tccaatcgca ggggttggtg ccaaactcaa gtccacaata ccaaactcca cacccagtct
     5161 ctcactggcc atttgaccaa ccaattgacc gatacgagtg attttaaagg cagttttctt
     5221 aactgtttcg gctactacat caaagctctg tccacgaact ttttccaaag cacgtttcac
     5281 cacaccagga ccagaaactc cgacattgat gataacatct gcttccccaa caccatgaaa
     5341 ggcacccgcc ataaatggat tgtcctcaac agcattagcg aatacaacca acttagccgc
     5401 tcccatatct gaaagatttg ccgtttcctt gataattcgt cccatatctg ccacagccgt
     5461 catattaata ccagacttgg ttgagccgat attgactgac gagcagacct tatccgtctc
     5521 agtcaaagcg cgaggaatgg aattgatgag aatctcatct cccttttgat aacctttttg
     5581 tactaaggca gaaaaaccac caataaagtc cacaccaatc tctttcgcag ccttatcaag
     5641 cgcttttgcc agaaccacgt agtccgtcgc atctgtcgct gccccaatca gagaaatagg
     5701 tgtcaccgat acacgcttat taacgatagg aattcccaac tcagccgcaa tttcatcacc
     5761 aacagctact aaattagccg cctttgtcgt aattttttga tagattttct ccgcagcacg
     5821 attgatatct ggatcgatac agtccaaaag agaaatcccc atggtaatgg ttctaatatc
     5881 gaagttttgc tcctcaatca tggcgatggt ttcagtaact tgtctaatat ccatgatgac
     5941 ctcctagata ttatacatag cttcgaaaat cgctgcactc tgaatattga tttttacatt
     6001 caaagtttgc ccaaaagctt caaattcatt acgaagatag gtaaaatctt gcttttcatc
     6061 actagataca acagccatca tcgtaaaata ttcatccaag acagtttgag agatatcgtc
     6121 aatattcaat cctaattctg caattttacc agaaacacct gcaacaattc cagatttatc
     6181 tttaccaaca acagttataa tggctttcat aagtaaactc caattcttct taaaataaga
     6241 aaccttaaca ttaaacagga ttcttttcaa atagtatagc ataattctaa cgaaaatact
     6301 caaaaacgcc tgcttacgaa gttgttctta agaaaaaaac aatttcgtaa acaagcgtct
     6361 actatcccaa cttatgatga gtgttcccgc taggaccgaa atcctagcgg tagaccagag
     6421 ctagactaag agcacaagac tccatcatca taacactcaa caaaattgat gattttatac
     6481 taattcgatg atcgccattg gcgctgcatc accacgacgt ggttcagttt taaggatacg
     6541 agtgtatcca ccgttacgtt cagcataacg aggtgcgatt tctgagaaca atttttgaag
     6601 tgctgtagta gaagtgtact tatcagttgc ttcatcatag ttttcagatg cgatttcatt
     6661 acgtacgaaa gcagctgctt gacgacgtgc atgcaaatca ccacgtttac ctagagtaat
     6721 cattttttca acagttttac ggatttcttt agcacgagct tcagttgtca cgattgattc
     6781 gttgatcaaa aggtcagttg tcaaatcgcg aagcattgct ttacgttgtg agctagtgcg
     6841 tcctagttta cggtaagcca tgtattcctc ctttatttat cttttaatcc aagacccaaa
     6901 tcaatgagtt tgagtttcac ttcttccaaa ctcttgcgtc caagatttcg tactttcatc
     6961 atctctgctt cagatttttc tgtcaaatca tgcacagtat tgataccggc acgttttaaa
     7021 cagttgtatg aacgcacaga caagtccagt tcctcaatcg tacgatctaa aatacggtcg
     7081 tcagattcag tatcagcttc tttcatcact tcagttgact tagcaatctc agtaagattt
     7141 gtaaacaaat caagatgttc tgtcaaaata cgtgctgaaa gccctaaagc atcttctgga
     7201 ataattgttc catttgtcaa gatttcaagg gttaatttgt cgaaaccatc attgctacct
     7261 acacgagcag gttccacttg atagttgact tttgtaactg gtgtataaat agaatctaca
     7321 gcaagtgttc caactggtgc attatccttt ttattttcat cagcaggtac atatccacga
     7381 ccactgttaa cagtcatagt cgcttttaga gaagaacctt caccaattgt aaagagataa
     7441 tgatctggat ttacaatttc aatatcgcta tctgtcaaaa tgtcaccagc tgttacttca
     7501 gcaggacctt caacatccag ttcgatgatt ttttcgtctt caacgtacga tttcactgca
     7561 attcctttaa tgttcagaat gatttgcatc acgtcttcac gaacacctgg aactgtgtca
     7621 aactcatgta acacaccatc aatgttgata gatgtcacag ctgctcctgg tagagaagct
     7681 agaagtacac gacgaagaga gttaccaaga gttgtaccgt agccacgttc aagtggttcg
     7741 attacaaact tgccataatc tttattttca tcaatttttg ttatatttgg tttttcaaac
     7801 tcgatcattt agttactccc tcttaaacga aaagcagtgt aatgcgatga ttatacacgg
     7861 cgacgttttg gaggacgagc accattgtgt ggcactggag tcacatcacg aattgctgtt
     7921 acttcaagac cagcggcagc aagcgcacga atagctgact cacgaccaga acctggacct
     7981 tttacagtaa cttcaactga tttaagaccg tgttcttgtg cagatttagc agcagcttca
     8041 gaagccattt gagcagcgaa tggtgtagat ttacgagaac ctttgaaacc aagagcacca
     8101 gctgatgacc aagcaattgc attaccatgc acatcagtaa tcataacaat agtgttatta
     8161 aatgtagcgt gaatatgagc aataccagat tcgatattct ttttcacacg acgtttacgt
     8221 gttggtttag ccaagacttt tacctcctat attatttttt cttaccagca atcgcaacag
     8281 ctttaccttt acgagtgcga gcgttgtttt tagtattttg tccacggaca ggaagtccac
     8341 gacggtgacg gataccacgg tatgaaccga tttccatcaa acgtttgatg ttcaagttta
     8401 cttcacgacg aaggtcacct tcaactttga ttgcatccac ttcacgacgg atagcatctt
     8461 cttgatctga tgtaagatca cgtacacgaa catcttctga gattccagca gcagccaaaa
     8521 ttttcttaga tgttgcaagt ccgataccat aaacataagt caatgagatt actacgcgtt
     8581 tgtcatttgg aatatcaact ccagcaatac gagccatgtt ttctcctttc tatcttatcc
     8641 ttgacgttgt ttgtgttttg gatttgctgg gcaaattacc ataacacgac cattacgacg
     8701 aataacttta cagtattcgc aaattggttt gaccgatggt cttactttca tttcttatcc
     8761 ctccaagttt ttcgattatt taaagcggta agtgatacgt ccacgtgtca agtcatatgg
     8821 actcatttcg acagtaacac gatctcccgc taaaatacga atatagtttt tacgaatttt
     8881 accagaaact gttgctaaaa tctgatgtcc attttcaagt tcaaccgtaa acattgcatt
     8941 cggcattgta tcaactactt tgccttcaac ttcaatcaca tcgtcttttg ccacgcaaaa
     9001 gcacctccat aaatttcgat tcgatgcctc tagacacaga gacaacaatt ataagtcaga
     9061 ctatctcagt ataacatttg tagcggattt ttgcaagtgc gaaaaacgct ttatttcaaa
     9121 tttgtcaata cttttt
//
LOCUS       DAVBBS010000071         8844 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVBBS010000071 DAVBBS010000000
VERSION     DAVBBS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8844
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.071"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(135..1103)
                     /locus_tag="VQU32_001886"
     CDS             complement(135..1103)
                     /locus_tag="VQU32_001886"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET2473287.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(1248..2063)
                     /locus_tag="VQU32_001887"
     CDS             complement(1248..2063)
                     /locus_tag="VQU32_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET2473288.1"
                     /translation="MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKKWELPKKVLILSLLGGMFLSGWLSSFTNTYIHDLLGVLFPD
                     SPFLNAFESAIVAPLVEEPLKLLPLVFVLALIPVRKLKSLFLLGIASGLGFQMIEDIG
                     YIRTDLPEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAHKEQKVGKKQGLI
                     FLGLALRTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(2088..2585)
                     /locus_tag="VQU32_001888"
     CDS             complement(2088..2585)
                     /locus_tag="VQU32_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET2473289.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(2658..4019)
                     /gene="radA"
                     /locus_tag="VQU32_001889"
     CDS             complement(2658..4019)
                     /gene="radA"
                     /locus_tag="VQU32_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET2473290.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MSVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(4033..4548)
                     /locus_tag="VQU32_001890"
     CDS             complement(4033..4548)
                     /locus_tag="VQU32_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2473291.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGFPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(4550..4993)
                     /locus_tag="VQU32_001891"
     CDS             complement(4550..4993)
                     /locus_tag="VQU32_001891"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET2473292.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(5050..5147)
                     /gene="ffs"
                     /locus_tag="VQU32_001892"
     ncRNA           complement(5050..5147)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VQU32_001892"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5180..5647)
                     /locus_tag="VQU32_001893"
     CDS             complement(5180..5647)
                     /locus_tag="VQU32_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET2473293.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(5848..7134)
                     /locus_tag="VQU32_001894"
     CDS             complement(5848..7134)
                     /locus_tag="VQU32_001894"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET2473294.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGIGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGARNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(7365..7601)
                     /locus_tag="VQU32_001895"
     CDS             complement(7365..7601)
                     /locus_tag="VQU32_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HET2473295.1"
                     /translation="MLQKIYEQMTNFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            7867..8664
                     /locus_tag="VQU32_001896"
                     /pseudo
     CDS             7867..8664
                     /locus_tag="VQU32_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8699..>8844)
                     /locus_tag="VQU32_001897"
     CDS             complement(8699..>8844)
                     /locus_tag="VQU32_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2473296.1"
                     /translation="KLLPLPPYSPEYNPIEKTWAYIKKNLKKVLPSCNTFYEALFSCS
                     CFN"
BASE COUNT         2836 a         2104 c         1425 g         2479 t
ORIGIN      
        1 tgatgacgta cagtcatatt tcttctcttt ttattatatc acagttttaa atctagcttt
       61 actagattca ccgctactat ctatttattc ggaaaaaaga cgaaaaaacc tgagaatcat
      121 ctcaggcttg gtcattaaat ttttttctca atatcgaaaa gtggagaaag tggtcgtttt
      181 tcatgaatac gtacgatagc atcccctagg agatgagcga ttgaaatctg ctcaatctta
      241 tcaatcaaac gctcttctgg cagatagatg gtatccaaaa caaccaattt cttaatagct
      301 gatttttgga tattgtccgt agcaggacca gaaagaactg ggtgcgtaca gcttgcatag
      361 acttcaacag caccagcttc cgcaagagca tctgccgcat gacaaatcgt tccagcggta
      421 tcaatcatat catcaatcaa gatacaagtc ttgccttcaa ccttaccgat gatgttcata
      481 acttcactag tattcatctt atcaacgcta cgacgtttat caataatagc gatagatgtt
      541 ttcaaaaatt ctgccaactt acgagcacga gtcacccctc catggtccgg gctgacaacc
      601 acatagtcag aaccaaccat accacgacgc tcaaaataat ctgcaatcag aggagcaccc
      661 atcaaatgat ccacaggaat atcaaagaat ccttgaattt gcgcagcatg caagtcgatg
      721 gtcaataaac gatccactcc agctacttca agcatatttg cgacaagttt tgaagtgatt
      781 ggctcacgcg ctctcgcctt tctatcctga cgtgcatacc catagtaagg catgacaaca
      841 ttgacagatt ctgcactcgc acgcttcaaa gcatctacca taatcaaaat ttcaagcaga
      901 ttgtcattta caggcgaact agttgattgt aggataaaga cgtgtttccc acggattgat
      961 tcttcaatgt tgacctgaat ctctccatct gaaaattggc gaacacttga tttccccaac
     1021 tctatcccaa tctcctgcgc cacacgttct gccaattctt tattagaaga aagggcaaac
     1081 agctttaaat cagaaaaaga catgatttcc tccggtatat atgtatcgct tgtgcttttc
     1141 acaagatttt ccatctacca ttgtagcgct ttttgcacta tttttcaatc aaaaataaaa
     1201 gaagggcacc atatttgtac ccttgcatca ttcttttgaa aaatattcta ggtcatcaac
     1261 tcattgtgtt tctcaacaaa gcaataagca tgataaaaac catagagagc aatagccgta
     1321 accactggaa tcgctaaagg caactctgtt tccaactcca caaaaggaga gttaaacaag
     1381 aagtgagttc tcaaggctaa acctagaaaa ataaggccct gtttcttgcc aaccttctgt
     1441 tctttatggg ctctgtaaag caagtaaaca cctactacag ctagacctga aaaagtccag
     1501 tgagaggcaa ttcctgagat gatacgctct aaaattcgcg aaatagtaaa gtcaaagccc
     1561 tctggcaaat ccgtacgaat ataaccaata tcctcaatca tttggaatcc caaaccggaa
     1621 gcaattccaa gtaaaaacaa agattttaat tttcgcacag gaatcaaagc caaaacaaaa
     1681 acaagtggca ataatttcaa gggttcttct accaaaggag ccacaatagc actttcaaag
     1741 gcatttaaaa atggactatc tgggaaaaga acccccagta aatcatggat ataagtatta
     1801 gtaaaactag acaaccagcc tgaaaggaac atccctccca ataaagacag aatcaaaacc
     1861 ttctttggca attcccattt tttcccaata cggaagagaa aataaagagc cggaatcatg
     1921 taaaagagag ctagaaagat agaaactccc attagtccat attccgcacc tgacctcgaa
     1981 ccgtccgtat agtagatggt ttcatactgt aaaccaatac atagcaataa aataaaaata
     2041 aataaaatac tgcttttctt catacacttt ctttctaaat gaagtattta taattctacg
     2101 actgtcatac ttcctgtatc aacattgtaa atggcaccag agataatgac atcgtctggt
     2161 attaggggag actcgataag cagttgcata tcctcgcgta cactctcttc tatatcttgg
     2221 aagggcaaga agtcctggtc tgacacatct acacctaatt cctcttttaa atactcctga
     2281 aaaggttcat tttcaaaggt ctgagcacca cagtctgtat ggtgcaatac cacaatctct
     2341 cttgtcccca tttgttgctg ggaaataact agcgaacgaa tcatgtcttc agtcactcga
     2401 ccacctgcat tccgcaagat atgagcatcc ccaagtgcca agcccagagc ttgcgcaacg
     2461 tgcagacgag agtccataca ggtcacaata gctactcttg ttttgggttt aagtggcaga
     2521 tttaactgcc catgtagggc aacataagcc tgattagctt gcataaactg ttcaaaatac
     2581 gacacgattc cctccttgaa aatttgatag tcaaatattt ctcctatctt atcattttta
     2641 agagaatttg tcacggatta tgcaaagacc tttttcaaaa cttcctgaat cgttgtcacg
     2701 ccaatgacct gaatttcctt aggcagagtg attcctgtca aggaattctt aggtacataa
     2761 atcttagtaa agcccagttt agcagcttcg ttgatgcgtt gctcaatacg attcacgcgc
     2821 cgaatctctc ctgtcaagcc cagttctccg acaaaacatt cctgaggatt agttggcttg
     2881 tctttgtagc tcgaagcaat agcaactgca acagccaagt caatggcagg ttcatccaat
     2941 ttaacaccac cagcagattt gagataggca tcctgatttt gcaagagaag ccctgcccgt
     3001 ttttccaaaa cagacataat caagctagca cggttaaaat caagtcctgt cgtagtacgc
     3061 ttggcatttc caaacatggt cggtgttacc aaagcctgaa cctccgccaa aatcggacgc
     3121 gtcccttcca tggttacaac gatggaggaa ccagtcgccc catccaaacg ctcttctagg
     3181 aaaacttgac tcggattgag tacctcaacc aagccgcccg actgcatctc aaaaatccca
     3241 atctcattag tggaaccaaa acgatttttg accgctctca aaatacgaaa ggtgtggtga
     3301 cgctcccctt caaagtaaag caccgtatcc accatatgct ccaacatacg aggcccagcc
     3361 aaggttcctt ctttggtcac atgacctacg ataaagatgg caatgttatt ggtcttggcc
     3421 aactgcatga gttcagcggt cacttcacgc acctgagaaa cagacccctg cacccctgaa
     3481 atctcaggag acatgatggt ctggatggaa tcaataatga gaaagtctgg ctggatacgc
     3541 tccacttctg cacgaacact ctgcatattg gtctctgcat agagataaaa ctcactatca
     3601 atatcaccta agcgctctgc acgtagttta atctgctggg cagactcctc cccactgaca
     3661 tagagaactg tccccacttg ggacaactgg gttgagactt gtaggagaag agttgatttc
     3721 ccaatcccag gatccccacc gatgaggacg agacttcctg gtaccactcc gcctccaagc
     3781 acacggttga attcctccat ctccgtcttg gttcgattga cattgatgga agtcacctca
     3841 gctagtttca tgggcttggt tttctcacct gtcaaggaca cacgcgcatt cttgacctcg
     3901 gcaacctcaa cctcttccac aaaagaagac caagacccac agttggggca acgtcccaga
     3961 tatttagggg aattataccc acaattttga catacaaatg tcgctttttt ctttgcgatg
     4021 acaaacctct ttctatatct ctaactcaca ctcaatcact tggcaaaaat caatcttctc
     4081 atttggcaca aactggcgca tgagcattcg atgagcaaca actaccacag tctgatgttc
     4141 tcgatactta gacatacatt ctagaaaccg agacttcatt tccgtagctg tctcatattg
     4201 aataggacta ttaggaagca actccccctt gttttctaaa aacagtcttc tagctgtttc
     4261 aaagttttct attcctgttt tatagacctg ccattcatgt aataaaggct ctactcttaa
     4321 aggaaaaccc gtagcacata ccacatacga agccgtttct aaagctcttg tgactgcaga
     4381 agatacgatt atttcagctg acgagagtaa aggatttttg ctcaatttct ggacttgctg
     4441 ccgtcccatc tcagacaagg gtgccaaatc tatcccaaat cctatataag aacgctcctc
     4501 taactcacgg taatctggct ccccatgacg tacaaagata atcttcattc tagtgccctg
     4561 tcgatccaaa tccaccagtt cgaacgccat cagccgcatc tccatctgca attaagaaag
     4621 tagcaaaaac agcctggaca atacgctccc caacttcaag aacaacctct tggtctgtga
     4681 tattcttcat ctgcgcaaaa atatgccctt catttccagg atttccataa taatccccat
     4741 caatgactcc aactgagtta attaaaacca agcccttctt acgaggattt gaagaacgat
     4801 catagaggta gagaacctca gtcggctgca tataagcctt aacccctgtc ggaaccaaga
     4861 caatctctcc tggcgcaaca actgtacgca cagcaacctt taagtcgtaa ccagccgcat
     4921 gcgctgtctc acgcttgggc aataaatttt catctgtaaa actcgaaacc aattcaaaac
     4981 cacgaatttt cataattttc tcttttctat tatcatttat tctagattat tctatcttat
     5041 ttattcggaa aaagcacgaa aaaaagagca cacaattcaa atcgcttagg gctgctggat
     5101 tcctcccctg acccgcttca cgcagaactg ttgctccact atttattata tcacattcct
     5161 attcatttta aaagcaaaat tattttttcc gtctatttct aaaaaaatcc tgcatgatag
     5221 ctgcgcattc atcttccaaa attcctattt caacctccac acgatgatta agacgctcat
     5281 ctgtcaagat atcgtacaaa cttccagcag cgccaaattt ctggttttta gccccataga
     5341 ccacatttgg aatgcgggca agcccaatcg ccccactaca catgacacaa ggttcaatgg
     5401 tcacaaaaag tgtgcaatcc agcaagcgcc agctctcctc actcaagttc gcatcctcta
     5461 tagccataat ttccgcatgc ataaccgctc gctgtaattc ctcacgcgca ttatgcccac
     5521 gaccaatgat ttccccatct ttgacaatca cacaaccaat tggaatttca tcgtgttcaa
     5581 gagcaatctc agcctctctc aaagcctccc tcataaagac ttctttttct tcaagcgtat
     5641 aatacatctc ttttctcttt tcctacttat cgattttatt attatatcat gaatcccaac
     5701 acaaaaaaag ccaccgaatg cggtgactct atagggagat tattatgaaa aagaaaagtt
     5761 taggatattt gttacaacaa gttaggaggt cttcttgtaa ctgtctatag tatacccgac
     5821 ctatcttaaa caaatcttaa aaatctctta ggaccaaaca ctttctaaaa tatttgtttg
     5881 ttcacgacca ggacctactg agaaagtaga aatacgaacg ccaaccaatt cactcacacg
     5941 acgaacatag ttacgcgcat tctcaggaag atcttccaaa ttgcgagctc cggtaatatc
     6001 ttctgaccaa cctggcaact cttcatagat aggcttgcaa cgtttcaatt gctcaagact
     6061 agctggatag tagtcaatac gttgaccgtc aagatcatag gccacacaaa ttttcacagt
     6121 atccaagccg ctcaaaacat caatagagtt caaagaaagg ttagtaatac cagaaacacg
     6181 acggctatga cgcatcacaa ctgagtcaaa ccaacctaca cgacgtggac gaccagttgt
     6241 tgtaccatat tcatgaccca cttcacggat acgttctccc acttcatcaa acaactcagt
     6301 tgggaaagga ccatctccta cacgactcgt ataagcttta catacaccta caaccttgtc
     6361 aatcttgctt ggaccgatac cagaaccaat tgtcacacca ccagctacag ggtttgatga
     6421 cgtaacaaat ggataagtac cttgatcgat atctagcata acaccttgtg caccttcaaa
     6481 aagcacacgt ttgccattat caagcgcatc attcaagata acagatgtat ctatcacgta
     6541 tttcttgatt tgttgaccat attcgtaata ttcttcaaaa atatcatcga aaacaatcgc
     6601 tttactgtca tacaattttt caaaaagacg attcttttca gcaaggttac gttctaaacg
     6661 ctcacggaaa atatctttat ctaaaagatc tgcaatacga attccaacac gagcagcctt
     6721 gtccatataa gctggaccaa ttcccttaat tgtcgtacca atcttattgt cgcccttagc
     6781 ttcttcttgc aagcgatcca actcgatatg ataaggcaaa ataacatgcg cacgatcaga
     6841 aatacgcaag ttatcagttg ttacaccttc ctcatgaaga tagctcaact cttttacaag
     6901 agatttagga tttacaacca taccattccc aatgacagat attttttcag ggaagaaaat
     6961 cccagatgga atcaagtgca acttaaattt cttaccgtca atcacaatcg tgtgaccagc
     7021 attatcacca ccttggtaac gtgcaattac ttctgcattc gctgaaagga agtctgtaat
     7081 cttcccttta ccttcatcac cccattgggt acctacaaca acaactgaag tcataatttt
     7141 gtctgagccc tcaggctctt cctttctcac atacatggca ggactctcac ctgcaattat
     7201 atcttacaat ttattataat aaaaaatcgc ctttttatca agaagaaaca atagaaagat
     7261 ttgctatttc caactattaa aaaatgattt agaaaaatta ctagctattt actattatct
     7321 ttccataaaa gagtaaatta gttcggaaat ttactaaaat tacctcaaca agaaataaaa
     7381 ccccgattca ttaccaattt ttcaagatac aaacgataag caacacgata atggtaaacg
     7441 ataaaatccc tacgacaacc aatgccatat ctcactaaat aataaattaa aaatttaaaa
     7501 tgaacatgtt cccagtcaaa attatcacca aatgtaggac catactcttc ttcaatacta
     7561 tcatagaaat tagtcatctg ctcataaatt ttttgtaaca taatcaaata ctccttttct
     7621 tttttataaa cttattctaa caaaaaattt tacacattca ctatcaattc ctgaattgtt
     7681 aaaataccag cctcaacaag ataaaaatag gaaaagttga caaaatagaa acaaatttgc
     7741 tacctaaatt tcaaacgatt aaagttctaa aaagaccgcc ttaaaacttt tgggtaaatc
     7801 ctattagaac tataatattt atatatatgt attatggttc tgattttcta gtataaaaaa
     7861 attcaaacta gaattcttct tagaaaaggc cgttttaagt gctatcattg ctcaaaaatg
     7921 gaggtcgctg agacttctat cgtcaagaag aatcatcaaa ttccttgtat catcaaccaa
     7981 aagattgctc agaagttaat tgaaaagact tctatgaccg acattgatca tcagttgtct
     8041 atttcaactt caactgtcat tcgcaagatc aataattttc actttgagca tgatttttcg
     8101 cgtcttcctg agattatgtc ctaggacgtt gaaacagtcc ggggagtgac tgtttcaatc
     8161 gggagatgga gatgagcttt attgctcagg actttgataa actcaatatc ataactgttc
     8221 ttgagagcag aacacaagcc atcatccgaa atcccatgaa tacaaggcta tcaagcgcta
     8281 ctggaagctc attcaacaag atagtcagaa actgagtgat aaacgatttt atcgccctac
     8341 ttttcgtatg cacttgacta ataaagaaat tctagataaa cttttgagct attccgaaga
     8401 cttgaaacac cactataatc tctatcagct cttgcttttc cacctctgtt tcagactttg
     8461 tttaaaacct tcctcaaaga taaagagaaa atcgtcaacg cccttcaatt accttattcc
     8521 aacgccaaac tggaagccac caataatctc atcaaactta tcaagcacaa tgcctttggt
     8581 tttaggaact ttgaaaactt caaaaaagaa aggacgaaat ttgtcctttc tagatcttcg
     8641 ctttcttcaa cccactacag ttgacaaaga accgaaaaat gtctcgcctc taatatagtc
     8701 aattgaaaca agaacaagag aaaagagcct cgtaaaaggt attgcaactt ggtaatacct
     8761 ttttgaggtt ctttttgata taagcccatg ttttctcaat aggattgtac tcaggtgagt
     8821 agggaggaag aggtaaaagt ttat
//
LOCUS       DAVBBS010000072         8830 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVBBS010000072 DAVBBS010000000
VERSION     DAVBBS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8830
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.072"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            264..1322
                     /locus_tag="VQU32_001898"
     CDS             264..1322
                     /locus_tag="VQU32_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473297.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIESKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREIALLLATIIVYFFTHRILPLSVFTFIFSYILLFAQ
                     SYLGGNTVWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNLTPIILLAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYD
                     NEALGDLSKQYLNRNISELQDGSFDGGIQDAVASKQIVVINEFIACLNSRCMPSQYDR
                     FFYKDRPYIFSRKSPIKG"
     gene            1637..2611
                     /locus_tag="VQU32_001899"
     CDS             1637..2611
                     /locus_tag="VQU32_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473298.1"
                     /translation="MKNKRYFFDTILMILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLIPFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLKKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSTK"
     gene            2991..3365
                     /locus_tag="VQU32_001900"
     CDS             2991..3365
                     /locus_tag="VQU32_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HET2473299.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
     gene            3573..4475
                     /locus_tag="VQU32_001901"
     CDS             3573..4475
                     /locus_tag="VQU32_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473300.1"
                     /translation="MIRKLIVFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEGLIKIFPFIFIRRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWQKEDSFESNLHKILMDKIQKSELSYSDLMSLLLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWQEKVDKIRIQLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            4531..4770
                     /locus_tag="VQU32_001902"
     CDS             4531..4770
                     /locus_tag="VQU32_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET2473301.1"
                     /translation="MELVLPNNYVVIDEEVMMYLDGGAYLSKRACQGICAAAGVLATA
                     AGKIAYCIGYGVLNRGCDINENPYPWDGFISATVR"
     gene            4767..5495
                     /locus_tag="VQU32_001903"
     CDS             4767..5495
                     /locus_tag="VQU32_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473302.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLVINSILLMSFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IRKDFDIKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGKVDNHVLTLI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKEKEYELFKNSK"
     gene            6630..6869
                     /locus_tag="VQU32_001904"
     CDS             6630..6869
                     /locus_tag="VQU32_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473303.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLYKITRDYASQTKPAIQDGVFILEDFRNILCTMM"
     gene            7419..>8830
                     /locus_tag="VQU32_001905"
     CDS             7419..>8830
                     /locus_tag="VQU32_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein"
                     /protein_id="HET2473304.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPVASQSKAEK
                     DYDAAVKKYEAAKKEYEDGKAAQKKYEDDQKKTEEKAEEERKASEEEQAANLKYQQEL
                     VKYIRENDPTKKAEAKKAMDEAEKEYKKKQTEFAEVRAKVIPSAEELKKTRQKAEEAK
                     AKEAELTKKVEEAEKKVTEAKQKVDAEHAEEVAPQAKIAELEHEVQKLEKALKEIDES
                     DSEDYVKEGLRAPLQFELDVKQAKLSKLEELSDKIDELDAEIAKLEKDVEDFKNSDGE
                     QAGQYLAAAEEDLVAKKAELEKTEADLKKAVNEPEKPAEETPAPAPKPEQPAEQPKPA
                     PAPQPEKPAEEPENPAPAPQPEKSADQQAEEDYARRSEEEYNRLTQQQPPKAEKPAPA
                     PQPGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNANGAMATGWLQYNGSWYYLNAN
                     GAMATGWLQYNDSWYYLNANG"
BASE COUNT         3046 a         1215 c         1616 g         2953 t
ORIGIN      
        1 taaatctaat aaattctatt attatagctc ttggtatgat attggcatta attttggtat
       61 tatcaagagt gtatagaaaa tatttttata gagacgatga tgactaaagt ttttatcaat
      121 aatttgggct ccttgtcaac tgtagtggat tgaagaaaag ctaacgtctg gggaagggtt
      181 taaagctttg ctttatcaaa tattgactta cagagttgct ctattgtatt gatgtattct
      241 tagtaaataa tatggtgttt tgaatgaaag atgatcagaa atatttatta gcgggacttt
      301 attcgcttct agttgcaata ttttattttc cattgattga gtccaagggg atttttgtaa
      361 gtatattgat ggcggtttta cttttatatt taatatactt cattgcaaca gtcattcata
      421 ttgtgataat taagtttata agaaaaaaat cttttaagta tttggttcta tatccattta
      481 cttatgatgg gagctggaga tttcaaccta taaacttact ctactttcca gaaatggtga
      541 gagatgtcat tccgattaat ttagttcaag aatattgcca aggacaacct tacggactgt
      601 taaaaaagat gctgaaaaga attcgattgt cacgagagat agctttactc ctggctacca
      661 ttatcgtcta tttttttacg catagaattc tacctctttc agtatttacg ttcatatttt
      721 cttacatatt gctatttgca caatcttatt tgggtggtaa taccgtttgg attggaaata
      781 gaagattaat aatagatgat gaatttgaaa aaatcttgct ttcaaaaagt tatataaaag
      841 aaatatcatc tgcacgatac tcggaatact tgacatgtga atacaaaaac cttacaccaa
      901 tcatattgtt agctattttt gaaaatttgc ttgactccta cttgcttcag aatcagagtg
      961 aggttgactt agatattttt tataaagttt tacctttgct ttataaagaa aaatatacta
     1021 tgggattcaa ttactttgta agtttaaatt atttactata taaggttgga tttttgggaa
     1081 tcatttacga taatgaagca ttaggagatt tgtcaaaaca atatttaaac agaaatattt
     1141 cggaattaca agatggttct ttcgatggcg gtatacaaga tgctgttgcc tctaaacaaa
     1201 tagtagttat aaatgaattt atagcttgtt taaattccag atgcatgcca tcccaatatg
     1261 atagattttt ttataaagat agaccttata ttttttccag aaagagtcct attaaggggt
     1321 gaaaatttgt tacatttgga ttaacaagtg tcctagggat tagaaataac tctagtatag
     1381 ctagatattt ttgagtaact agaaatatca ttaggctacc catatgattg ccagaaaaga
     1441 tggctgatga atcttattta tactgttgca gtaggcattg tctaggataa ataagctatc
     1501 ttgatggggg aaattggaat aattttagta gaaacattaa aggattatgg acaagtagcg
     1561 caggattagg tgatgcaatg tgctaagttg gcttggtgtt agcagtagta ttatttaaca
     1621 atggaaagta gactaaatga aaaataagcg ttattttttt gacacaattt taatgatttt
     1681 actgcttata agcacgatat tttgtgtcag cccagtattt ataaaattgg acatcttagg
     1741 aaccccctct catgccatct taacctttgt attggcgata ccgttattct atattttgtc
     1801 tcaatgctta catactttgc tattactagt ttcctcaatc ttttgtaaat taagaccaat
     1861 ttatttttat tttatatttg tgatcattat aggagctagg aaatactatc gaattctctt
     1921 tcaccaacta atgggattta gtccaggaat agcagtgttt tataaggaga gtcaaacaac
     1981 taagaatctg tttaaatttt actacttctt gtactttaca acactaattt catactattt
     2041 tttctttact tttgtgtatg acaaaccgtt gcttttgcct cttattccat tttctattat
     2101 catagcatta gtacaaaaac tttatagaat tgagaatcaa caattatttc tgttaaaatc
     2161 aaaggtacta acaatactgg aatctaagaa gaattgtgag tttaatcttc aggattatca
     2221 tgaaatttgg aaattgcaat caaaatctga gttgccatgc gtggctttat cttatatcag
     2281 tttgatcaag ccatatcttt cagagagcgt gcgtgaacaa attgatttat tagaagttaa
     2341 gcgatttaaa aaaataaatc atccaatatc actttatggt atgttggatg ttataaaatt
     2401 aaatctatat ttacggcatt ataatgaaaa aaataaatat gaatcaatgt taaagaaaat
     2461 tttagaggta agaccagact ttgttttaat cgagcagaat attgatgata gtttaaatag
     2521 ctcgcaacca ttatctttat ctttagccat ttcagaaatc caacttttgc ttgaagtgta
     2581 tatggggatc aaacatgttt caaccaagtg ataaaaccag aataatattt gccattatag
     2641 gggcaagttg catattagtc agtatgtatc ttattccact tattaaaact gagaataaat
     2701 tttgggaaat acttttattt atattaggtg tattgctaat tggaattgcg ttactaagtc
     2761 aaaaaaaata aattttgaaa tagtattagt gagttcgcaa tcatttattg ctagtggttt
     2821 agaactgtta agtttcaaac aataattgat tttaaacctt aggtaaccta taaaaacaaa
     2881 taaggtagag aaaggatatc ttatctaggt agctaagtga taaaaacaca gtcgtctatc
     2941 acttgttagg aaactatcct aactatgctt aataaaaaag gagaaaataa atggaactcg
     3001 tattaccaaa taattatgtt gttcttgagc aagaagagat gatgtatctt gatgggggat
     3061 tttctattcc gagatggcct gttgcaacag ccattaatat agcttttaat ggtgttttag
     3121 gtggaggagc aatcagtcta gttagaaatt atattcgtaa ttatggtttg cggcgagtta
     3181 caagcgcaat tgctggagca gctgcaagat atgttggggt acgagttgca aatagagtgg
     3241 caggatttgc actgtctgct attaatggat ttgcagcttg gatgtcaatt ggcgatgcta
     3301 ttacaacaat ctgggccaac aatgatgtaa ataggagaga cccaaattta aacgccttgt
     3361 ggtaactaga tatgggtatt aaaatggaaa aaatttttgt tattattttt tttgtatgtc
     3421 tatttatatc atccataact tttttagcct atgattttgt tagcgaagaa ataaaaaagt
     3481 tgattatttg gattaatgtc gtttttttga ttttaatcat agcaatgatg atttatccaa
     3541 aattaagaaa atgataagga cactgtaaaa ttatgatacg taaactaatt gttttcttac
     3601 taatgctccc tctttttgtc ttgtggctag agttgcatgt cctagtgaat aacttactat
     3661 tgaatctaga atctcccctt gattttgtga ttagtatgag tcttgctttc tgtagtttga
     3721 ttttatcaaa aatagtttta gacatactat acgcattgaa agatttatac aagaaagaag
     3781 gtcttattaa gatttttccc tttattttta taagaaggaa aaaagtgaat gtgagattct
     3841 cgccctattt ttcattccat cgtaaaagtt tgtcgcctga tgatttaaga tctaggatta
     3901 tatggagttt tattcttgaa attgctatta tcctagtatt tattttaaaa attccttttg
     3961 caatcatcat gcttactaca atatttttct ggactataat ggatatcaat catctcgttt
     4021 ttaataagac ggaatttctt tttaatcaaa acaaatggca aaaagaagat tcatttgaaa
     4081 gtaatttgca taagatattg atggacaaaa tacaaaaatc agaattaagc tattctgact
     4141 taatgtccct tcttctctat gatgcgatga accaatccac ctttttaacg gatagtgaac
     4201 tttttgagga tattttgaaa aaaatcgaag attctcataa cacccttctg tgtacaggtc
     4261 ttgtcgagtt actgctgtat gagatttcta ttagtaacaa taacaactgg caagaaaagg
     4321 ttgataaaat tagaatacag ctcatcagaa tcaatcaatt agattttttc tattatacaa
     4381 gttggttaag gcaaaatttt gatttctgta tgaatagaga gtatcataaa atgaaatctc
     4441 ggaaattatt actgagtaat aaaaagatag tttaagttcg tcctatttgt aaaacaaatg
     4501 aaacaatttc aactaaggag gagaaaacaa atggaacttg tattaccaaa taattatgtt
     4561 gtgattgatg aagaagtaat gatgtatctt gatgggggag cttatttaag caagcgtgct
     4621 tgtcaaggaa tttgcgcagc agcaggtgta ttggctacag cagcagggaa aatagcttac
     4681 tgtattggat atggtgttct taatagaggt tgtgatatta acgagaaccc ttatccttgg
     4741 gatggattca tatctgcaac agtaagatga gtaatgtaga taagattcga aaaatacaca
     4801 tcatcgtgtg ttgggtgtat atatttttat cattcagggc gattataaat gatacggagt
     4861 attttttgct tattttttta gcttttatct actcaatagt atcactacct ctatattctg
     4921 tgaaaaataa aatagtatct atctgtctag ttataaattc gattctgtta atgagtttcc
     4981 ccattttaat caataagttt ttcccagaaa gttttttgac ttataccgta ttaataagtg
     5041 tttttatcct agagttagca atctttcatt taattaggaa agattttgat attaaattga
     5101 ctaacgaata taagaaaatt agtcagttta gaagcaaggt gtctcaatct ccttggataa
     5161 aatatttaga gatttctagt tttatattaa ctatatttcc atctattctt tatggtaaag
     5221 ttgataatca tgtactgact cttatctttt tgataaaaat ttgtgtagat actacgataa
     5281 aatttttatt catcagatta tttgacacaa gtactttaat gaagaggaga atattttttc
     5341 ttttcgcatt ggatgttata gcttacttat ttttaggata tcttttagtg attcaaaaag
     5401 caggctattt gttttcggtt ttacttcttt tttctaattt ttcagttcca tttatcaaag
     5461 aaaaggaata cgaattattt aaaaatagca agtaatagaa atatggatta aatcttagaa
     5521 cggcttaaaa tcagatatga taaagggaaa tagtcttatc taggtaacta agtgctacaa
     5581 acacaatcga ggatcacttg ttaggattac attttaatca tgcttaataa gaatggagaa
     5641 ttcctatggg gattgtatta ctctgaagtt tcgaatacct tagttctcgt gattttgagc
     5701 tattctagct ttagaatcct tcagaacgta aaattttaag cagtcaaaga cttagaatag
     5761 cctgaaaaca tttattttat agcaattcta ggcttagaat tgctcatata tatgtatttg
     5821 agctgctcga tagttaggac agctctattc tgagaaatct gagctatcaa tgcttcagaa
     5881 tagtatttcg ccaatggttt ttattgaaga ggtgattgtg ttatgatagt ctaaaataaa
     5941 attgatttaa tagagtaaat ggagaatcgt tacatgaaac atttttttgc aggaattggt
     6001 gagataaaac ttgttggcta cttattgtat atatgtgtag gactagcccc tctttttcat
     6061 atatatgtca taggttcgga actgagtttt gtgaaaatag tgttatctat tttgggagtt
     6121 atatttgtaa gtatgttaac tattgctcga atttatcgga cttttctata aagaatagtt
     6181 tggacttaaa ctgtatctaa aaacagaggt tggtcgatta tcagcctctt tcttgtcgtt
     6241 gaaaagagtg agatatattg acttttgaaa aaaagatgtc ataattgagt tgggataaac
     6301 ctaaaagtaa tatagtagat tgaatctgga atagtccgtt atgactgtta aaatatttct
     6361 agaaattaat ttgactttcc taatcgattt gttcacatct tgtttcaatc tactatactt
     6421 tctcgttaaa ccgttcggca attcatggtg acatggacac atagtagtaa gcacactatg
     6481 tggcttggca gagctaaaaa ctgttccctt gcgataagcc taataagcac aacataggga
     6541 gttagagaag ccgactctaa tcaaccactt tgggcaatag tgagaactgc cccgtgcttt
     6601 tttattttga gaaaatatgg agtttgtcgt tgaaattact tgattgtatt ttggactatc
     6661 aagaaagatt caatggaaaa acatgtcaag tatcaacgaa ttataagtat ttagagattt
     6721 tcaaagtgaa tttttgtttg actgatttac atcatttatt tgacttatac aaaatcacac
     6781 gagattatgc tagtcaaaca aaacctgcta ttcaagatgg tgtttttatt ttagaagatt
     6841 ttagaaatat cctatgtaca atgatgtaat cgaaaggata tctttatgtg agtttatcgg
     6901 cgatatcttc tatatctaag ctagcaagtt gttgcatcgt agctaaggat ttatctaaaa
     6961 atactatgaa attggacgtc atattttttg aggataaaaa taaaagatcc gcagttttag
     7021 gtttacgaag agacaaaagc ggagtattta aaccagttac tctacatttt acaagcgcta
     7081 agaaatatgc taaagttcgt aaaacagatg tgaaagaaat gaaatggcta taaagattgt
     7141 ccgcaggctt aagcttgcgt tttttgttgt ttaacgaaat taaaaaacaa gctctctcat
     7201 cggaaatgtt tttataggaa tgaaggaaga tgatgctatg tttaaaaaga gaatcgaaaa
     7261 gggcaaaagt agtgtcttta tctttttgga gtagaagctt atgatataga aatttgtaat
     7321 aaaaatgtaa tataaaacat ttgacaaata tttacggggg gagtatactt aatataagta
     7381 tagtctaaaa atgactatca gaaaagaggt aaatttagat gaataagaaa aaaatgattt
     7441 taacaagtct agccagcgtc gctatcttag gggctggttt tgttacgtct caacctactt
     7501 ttgtaagagc agaagaagct cccgtagcta gtcagtctaa agctgagaaa gactatgatg
     7561 cagcagtgaa aaaatatgaa gctgctaaga aggaatatga ggacggaaaa gctgctcaga
     7621 aaaagtatga agatgatcag aagaaaactg aggagaaagc ggaagaagaa agaaaagctt
     7681 ctgaagaaga acaagctgca aatctgaaat atcaacaaga gttggttaaa tatatacgtg
     7741 aaaatgatcc aacaaaaaaa gctgaagcta agaaagcaat ggatgaggct gagaaagagt
     7801 ataagaaaaa acaaacggaa tttgctgaag ttcgtgcaaa ggtaattcct agcgcggaag
     7861 aattaaaaaa gactagacaa aaagcagaag aggctaaagc aaaagaagca gaacttacta
     7921 aaaaagtaga agaagctgag aaaaaagtta ctgaagccaa acaaaaagtg gatgcagaac
     7981 atgctgaaga agtcgctcct caagctaaaa tcgctgaatt ggaacatgaa gttcagaaac
     8041 tagaaaaagc tctcaaagag attgatgagt ctgattcaga agattatgtt aaagaaggtc
     8101 tccgtgctcc tcttcaattt gaattggatg ttaagcaagc taaactatca aaacttgaag
     8161 agttgagtga taagattgat gagttagacg ctgaaattgc aaaacttgaa aaagatgtag
     8221 aagatttcaa aaactcagac ggtgagcaag ctggacaata ccttgctgca gctgaagaag
     8281 acttagttgc taaaaaagct gaattagaaa aaactgaagc tgaccttaag aaagcagtta
     8341 atgagccaga aaaaccagct gaagaaactc cagctccagc accaaaacca gagcaaccag
     8401 ctgaacaacc aaaaccagcg ccggctcctc aaccagaaaa accagctgaa gagcctgaga
     8461 atccagctcc agcaccacaa ccagagaagt cagcagatca acaagctgaa gaagactatg
     8521 ctcgtagatc agaagaagaa tataatcgct tgacccaaca gcaaccgcca aaagcagaaa
     8581 aaccagctcc tgcaccacaa ccaggttgga aacaagaaaa cggtatgtgg tacttctaca
     8641 atactgatgg ttcaatggct acaggttggc tacaaaacaa cggttcatgg tactacctca
     8701 acgctaatgg tgctatggct acaggttggc tccaatacaa cggttcatgg tactacctca
     8761 acgctaatgg tgctatggct acaggttggc tccaatacaa cgattcatgg tattacctaa
     8821 acgctaacgg
//
LOCUS       DAVBBS010000073         8550 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVBBS010000073 DAVBBS010000000
VERSION     DAVBBS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8550
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.073"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..128
                     /locus_tag="VQU32_001906"
     CDS             <1..128
                     /locus_tag="VQU32_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2473305.1"
                     /translation="FQSLNQLEQAIVDYIDYYNNKRIKVKLKGLSPVQYRTKSFG"
     gene            complement(257..460)
                     /locus_tag="VQU32_001907"
     CDS             complement(257..460)
                     /locus_tag="VQU32_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2473306.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(453..1535)
                     /locus_tag="VQU32_001908"
     CDS             complement(453..1535)
                     /locus_tag="VQU32_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET2473307.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1661..2443)
                     /locus_tag="VQU32_001909"
     CDS             complement(1661..2443)
                     /locus_tag="VQU32_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET2473308.1"
                     /translation="MVYTSLSSKDSNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2443..3786)
                     /locus_tag="VQU32_001910"
     CDS             complement(2443..3786)
                     /locus_tag="VQU32_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET2473309.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQSL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(3885..5201)
                     /locus_tag="VQU32_001911"
     CDS             complement(3885..5201)
                     /locus_tag="VQU32_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HET2473310.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5394..6615
                     /locus_tag="VQU32_001912"
                     /pseudo
     CDS             5394..6615
                     /locus_tag="VQU32_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
     gene            6814..8388
                     /locus_tag="VQU32_001913"
     CDS             6814..8388
                     /locus_tag="VQU32_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2473311.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
BASE COUNT         2454 a         1652 c         1892 g         2552 t
ORIGIN      
        1 cgtttcagtc gcttaatcaa ttggaacaag ctattgtaga ctatattgat tactacaaca
       61 ataaacgaat taaggtaaaa ctaaaaggac ttagtcctgt gcaatacaga actaaatcct
      121 tcggataaat taattgtcta actgtttggg ggcagtacat attcaacacc tgatactatg
      181 cgttttattg tgggaagatt ttcctcattt tctagtgaaa ttgaactttt tctaacatct
      241 tttgatagat gacttcttaa tcaattatca tttccattgt tgtttcttgg ggtttcatcc
      301 cttgtctttg ataaaagcgg agagcaccct cgttatcatt ccagacactt agggttagat
      361 tattgcagcc aatttcttta gcataatagc agaaaaatga caataaaata gcccctagat
      421 attttgaaag gaaagcatag gggctatata gtttaatcaa aataaagcaa atctttgctg
      481 gtgcttgtaa agaggtcgaa gccatccttg gtaacaacac cgcagtcttc aatacgaaca
      541 ccgactttac cagggatata gatacctggt tcaacagaga agcacatgcc ttcttcgatg
      601 accatgtcgt ttccttccat gatagatggg aattcatgga catccatacc gataccatgc
      661 ccgagacggt ggttgaagta ctcaccataa ccagcttttt cgatgacctc acgggcagcg
      721 cggtccactt catgagcagt cacacctggc ttgataaagt caagagcagc ttgttgggct
      781 tcaagagtca agttgtaaat atctttcttg aattggtctg gtttgccgac agcgactgta
      841 cgagtcatat ctgacgcata gccattgacc agaacaccca ggtcaaagag gagaagagca
      901 tcattttcaa ccttattagc tgctggaatg ccgtgtggat tcgcagcatt atcaccagtc
      961 aagaccatgg tatcaaagct catttcataa ccttcacgtt tcatggcaaa gtcgatttgt
     1021 gcgatgatat ctgtctcagt cttatcaaga gaaatattgt caaaaccaac atgaacagcc
     1081 ttgtcagcat aaagacctgc aaccatcatt ttttgcactt catcagctga tttgatgagg
     1141 cgcatgcgtt ggatacgagg agtgaggttg tcaaactcag cagtctcaaa aactgttttc
     1201 aaaccatggt atttggtcaa gatgagattg tcaaactcaa cagcgacacg tttgaagtca
     1261 agttgtggaa gagcatgttt gattttttgc catggatttt cagaatcgac atagcccact
     1321 actgggaagg aaacggtgct acttgcacgt tctacttcaa gagctgggac aaagaggaga
     1381 ggttcctgat ccgctaggac aaagaggaac atttggcgtt catggggatc actgtaaaaa
     1441 ccagtgaggt aattgattgt gacggggtca gatacgacag cgacgtctag tttttctgat
     1501 tcaagatatg ttaggatttg ttgtaattta gacatatgct accttctttc tacccctcta
     1561 ttttggcaaa aaatgagaga aaatgcaagg gagaagggta aaagaaaaga caaaaaactc
     1621 cgacaagata acggagttct ttgatatcat tttcctttgt ttaagaaaag tcagccttgc
     1681 ttttgacgtc actgtactct tcagcgattt cttggctgag aatgtcctca taggcaggga
     1741 gttggatgtc ctgaccatat ttcttcttga gggcagtagt aactaggcga taagccagat
     1801 tggcattacg agagaggata ggcccgtgga agtaggaacc aaagacattc ttataatgaa
     1861 ccccttcacc gaccttttct tcgttgtttc catttccata gacaacctgt cccagcggtt
     1921 tttggtcatc agagaggaag gtacgacctt ggtgattttc aaatccatag taggtttcat
     1981 cgaaatcttc attgtgaatc ttgatgtcac cgataaaacg gttattggtc tggttgagcg
     2041 tgtagtgtcc catgacccct agcccttcga tacgttttcc tgaagcttca acataatatt
     2101 gacccaatag ttggaaacca ccgcagatag ccagaactac accgtcgttt tggatgtagt
     2161 tgtcaatgct ctctttttta gcaggtaggt cgtctgcaat gatactttgt tcaaagtctt
     2221 gaccaccacc gaaaaaggcg atgtcgtagt gattttcatc aaagtcatca tggagagaaa
     2281 cgatgtcaac ggtcacatgg gctcccagtt tttcagccac atacttgagc atgaggatgt
     2341 ttccattgtc cccgtaggta ttcatgagat ttccgtagag gtgggcaatg ttgagctgat
     2401 aggggtaatt gctatctttt gaggaaagtg aagtataaac cattagttca tctcctttct
     2461 aacaatctga cgactagcca gcagttcacg aaattccagc atggcagtat aagttgccag
     2521 aatataggca tgcttgcagt cttgattctc aatggtcttg agaacttgct ccagattact
     2581 cgtttcagtg attttctcag ctggatagcc agtcactcgg aggcgacgag cgatttcaga
     2641 atgacgaaca ccgccagcgt tgatttcagg aatgtccatg tcagtgattt gctcaaagtc
     2701 tgcatcccag atccagctag tgtcaattcc atctgcatag ttggcattaa ggaggacaga
     2761 taggctaaat ggataaggtg ctagtttgat catttcgata gcttgggttg caccgactgg
     2821 atttttaatc aagacaaggg tacattcctt gtcaccgata tgaaaggttt cttggcgtcc
     2881 aaagacagca cggctcttgt caaatccctg tttgatgagt tgggaatctg cgccgaggaa
     2941 acgggcgatg gccacagcag ctagggcgtt atagatatta tagagcccgc cgatttggat
     3001 accgtattct tggccgtcta tgacaaagcg agagcgattg ttggtcaact caaccagttt
     3061 tgtcaaacga tagtcgagat caggacgttt acatccacaa ccttcacaga tataggcacc
     3121 caagtttgca taggtattat gctcatattt gaggatgcct tggcagtcag gacagagaat
     3181 cccttcggta ttgtagtgag ccagttgggc tggtcctttt tccaagtcaa aaccaaaata
     3241 ctctataggg tttggaatag ttggcttgta gaaaagtgga ctgtctccgt taaggagaac
     3301 agtagcagtt ggaacttttc gaatggcatc caatatcatg ttataggtag tatagatttc
     3361 accgaaacgg tccatctggt cacggaagat attagtaatg acaaaaagac taggctggat
     3421 atagtcacag atacgagata gactggcttc gtcaatttcg aggacggcaa tatttttccc
     3481 agtttttgaa gatttggctg ttaggaaggt tgttgcaatc cctgtaatca tgttggcacc
     3541 gcttgggttg gttagaactt gaccataaac ctcttttaaa atgccgacag tgagggcagt
     3601 tgtcagggtt tttccatttg ttccagtgac aacgacaatc tcgtagttct tagctaggct
     3661 ttgtaaaata tctttatcaa attgaagggc gactttccct gggagcgtac ttccacgtcc
     3721 aagacggctt aaaacgaagt gggaagaacg cccagcaaga aggcccaaag tagtttttaa
     3781 gttcatgttt ctattatatc acaaaagagg gaaaagacag atttggtgtt tcgtagaagt
     3841 aaaaacagcc caaagagggc tgtcagttaa gatgagattt caacttaaat cgcaaacaag
     3901 tcattcaagt tctcagccaa ggttgggtga gtgaagattt gttttgtgaa gtaagtgtaa
     3961 ggaatcttgt tgtccatagc aacagtaatg atgttgatga tttcttgaga accttctgag
     4021 aagatgcttg caccaagaat ttcttttgtt tcagtattaa caacagcttt gaaagctccg
     4081 cgaaggtctc catttacgtg accacgaggc atggctgcaa cagggatttc tttcactgcg
     4141 tatggaagtt tcaaatcagc tgcttggctt tcagtcaaac caacttgtga aagtgcaggt
     4201 gtgatgaaca tagtatttgg cacattgaga cggtcctcaa gtgtgtagct gccatctcca
     4261 gcaaggtagc tgtaaacaac acggaagtca tcaagtgaaa tgtaagtaaa ttgaaggcca
     4321 ccgttgacat ctccaactgc aaagacacca ggaacgtttg tttgacagtg tttgtctact
     4381 ttaatagcac cacgttcagt tagttcaata tctgtatttt caagttgaag tggttctaca
     4441 tttggtttgc gtccagttgc gtagagaagg gcgtcgaaac ggtaagtttc gtcttcagtt
     4501 acgacaagca cttggtcacc atcgtttttg atttcagtag tatggatatt ttgaagcaat
     4561 tcaatgccat cttcttccat gtattgttta gcaagagctg cgatggaagg ttctgcacga
     4621 ggtaggaatg tatccaaggc atctaggact gtgaccttgc ttccaagttt gttgtaaagg
     4681 ccggcaaatt caagaccgat atttccgcca ccaaggattc caagtttttc aggtaatttg
     4741 tccaagcttt ggatacctgt tgagtcaaag atgtttttgc ttgtagcaag tccaggaatt
     4801 ggcaagacgt ttgaaacagc accagtgttg atgacgattg tttcagcagt cagttctttc
     4861 ttttcatcac cagcttggat ttcgatgact ttatttgaaa ggaagtgagc ttccgcatca
     4921 aagatatcta cgcctgtacc agcaacagtc gcatagtttt taccgttgag gcgaccagtg
     4981 atcgtgtttt tagtagcaat cacttcttca aaagacaagt ccttttcagc agcaactagc
     5041 aaggttttag ttgggataca accaatgttg atacaagttc caccgtacat agccttgctg
     5101 cgttcaacga gggcaacttt tttgccagct gaagccaatt tacctgctag tgttttacca
     5161 gctttaccaa atccaataac aattaaatcg tatgttaaca tttatcttcc tcctattcga
     5221 atttcattct agcacaagaa aaaaactttt gcacaattct gctacggaaa agacagaaaa
     5281 agataaaaac gctttccaga caaaaaacta gtaatttttt gacaagcttg ataaactaaa
     5341 atcctatcta tttttatata agaaagaata aatgttcgaa aataggaggt tttatgaaat
     5401 cgaatcgtta tattattgcc tttgctgggg tcattttaca cttaatgctg ggttcgactt
     5461 atgcttggag tgtttatcgt aaccctatta ttgaaaaaac gggatgggat caggcttctg
     5521 ttgccttcgc ctttagtcta gcaatctttt gtttgggctt atcggctgca tttatggggc
     5581 gtttggtaga aaaatttggt ccgaaagtca tggggagtct atctgctttt ctatacgcag
     5641 gtggaaatat cttaacagga tttgcaatag accgtcagga actgtggttg ttgtatctag
     5701 cttatggcat tttaggtggg cttggtttgg gagcaggcta tattacccct gtgtcaacga
     5761 ttataaaatg gtttcctgat aaacgtggtc tcgcaacagg tttagcgatt atggggtttg
     5821 gttttgcttc tttattgact agtcccatag cgcaacacct catcgcaggg gtagggattg
     5881 tagaaacttt ttatatttta ggagcaagtt actttattat catgctccta gcttcacaat
     5941 tcattaagcg tccaaatgag caagagcttg caattttatc ttcttcaggg aaagaaaaaa
     6001 cagcctcttt gacgcaagga atggctgcaa atcaggctct aaaaagcaat cggttttata
     6061 tgctttggat tattttcttt atcaacatag cttgtggttt aggcttaatt tcagcggcat
     6121 cgccaatggc acaggagatg gctggcttgt ctacaagtca tgcagcagta atggtgggtg
     6181 ttttggggat tttcaatgga tttggtcgct tgctctgggc gagtttgtct gactatatcg
     6241 gtcgccctct aacctttagt atattactgc ttgtcaatct tttctctctc actttggctc
     6301 tttacagatt ccgttttatt tgtagttgct atgtctattt tgatgacttg ctatggagct
     6361 ggtttttctt tgattccagc ttatctcagt gatatttttg gaaccaagga attggccgct
     6421 ctgcatggtt atattttaac agcttgggca atggctggtt tagcgggacc tattttatta
     6481 gcagagactt ataaaatggc tcattcgtac acacaaacct tgttcgtttt tctcatttta
     6541 tacagtatcg ccttggcttt gtcttattat ctaggtcgtt caatcaaaaa agaaagtcaa
     6601 aaaccgctta catgattttc tgtagaaatg gggctatctt ttgctctggt cttgtgttat
     6661 actagatagg ttgcaaagaa aacagtactt ttcttttgtg gaaaagaagc aacacgattt
     6721 tatatctagt tatatgaaaa tacgtcataa aaagaaaagt ataaactaag ccctataggg
     6781 tggcttcgca ccacctttag aaagaagaat aacgtgaaat ttaatgaatt aaacttgtct
     6841 gctgatttgc tagcagaaat tgaaaaagct ggttttgtag aagctagtcc tatccaagaa
     6901 caaactattc ctttggccct tgaaggcaag gatgttatcg gtcaagctca gactggtaca
     6961 ggaaaaactg cagcctttgg cttgcctacc cttgaaaaaa tccgtacaga agaagcgact
     7021 atccaagctt tggtcatcgc tccaactcgt gaactagctg tccaaagtca agaagaactc
     7081 ttccgctttg gtcgtagtaa gggagtcaaa gtccgttcag tatatggcgg atcaagcatt
     7141 gaaaaacaaa ttaaggctct taaatctggt gcccatatcg tggtgggaac tccaggtcgc
     7201 ctcttggact tgattaaacg caaggccttg aaattacaag acattgaaac ccttatcctt
     7261 gacgaagcgg atgaaatgct taacatgggc ttccttgaag acatcgaagc cattatttcc
     7321 cgtgtacctg agaaccgtca aactttgctt ttctcagcaa ctatgccaga tgccatcaaa
     7381 cgtatcggtg ttcagtttat gaaagcccct gaacatgtca agattgcggc taaggaattg
     7441 acaacagaat tggttgacca gtactatatc cgtgttaagg aacaagaaaa atttgacacc
     7501 atgactcgtc tcatggatgt ggcacaacca gaactcgcta ttgtatttgg tcgtaccaaa
     7561 cgccgtgtgg atgaattgac tcgtggtttg aaaattcgtg gcttccgtgc agaaggaatt
     7621 catggcgacc tagaccaaaa caaacgtctt cgtgtccttc gtgactttaa aaatggcaat
     7681 cttgatgttt tggttgcgac agacgttgca gcgcgtggtt tggatatttc aggtgtgacc
     7741 catgtctaca actacgatat tccacaagat cctgagagtt atgttcaccg tatcggtcgt
     7801 acaggtcgtg ctggtaagtc aggtcaatct attacttttg ttgctccaaa cgaaatgggt
     7861 taccttcaaa tcattgaaaa cttgactaag aaacgcatga aaggtctcaa acctgcaagt
     7921 gtagaagaat ccttccaatc aaaaaaacag gtagctctca agaaaatcga acgtgatttt
     7981 gcagatgaaa ccattcgtgc caactttgag aaatttggta aggatgctcg caaattggct
     8041 gctgagttta ctccagaaga attggcaatg tatatcttga gtctgacagt ccaagaccca
     8101 gatagccttc cagaagtgga gattgcacgt gaaaaaccac taccgtttaa accatcaggt
     8161 aatggtttcg gtggtaaagc taagggaggt cgtggaggcc gtcgtgggga cgaccgtcga
     8221 gagcgtgatc gccgtggcaa tggtcgccgt gatgagttca aaaaaggaag tcgtggcaac
     8281 gatcgttttg ataaggaaaa acgttaccgt aaggataata aaaaaccacg caatacttta
     8341 agcgaaaagc aaacaggctt tgttattcgt aacaaaggcg ataaataaga aaaagcggtt
     8401 caaaatgaat cgctttttta tataaggaga ccgaatgtga aaagacaaat aaaattacta
     8461 tattatcttt tataatgtta tgatacacaa aaataggcaa cttgtcaata gaaaaggaac
     8521 aatttaatta aaaatattct ggtttttaat
//
LOCUS       DAVBBS010000074         8107 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVBBS010000074 DAVBBS010000000
VERSION     DAVBBS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8107
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.074"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            120..497
                     /locus_tag="VQU32_001914"
     CDS             120..497
                     /locus_tag="VQU32_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET2473312.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIALV
                     YAIAIVDSNYFSDDLAFHSFIVK"
     gene            732..2123
                     /locus_tag="VQU32_001915"
     CDS             732..2123
                     /locus_tag="VQU32_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET2473313.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIDQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            2237..3079
                     /locus_tag="VQU32_001916"
     CDS             2237..3079
                     /locus_tag="VQU32_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET2473314.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQEQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            3352..4113
                     /locus_tag="VQU32_001917"
     CDS             3352..4113
                     /locus_tag="VQU32_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET2473315.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            4483..5400
                     /gene="glyQ"
                     /locus_tag="VQU32_001918"
     CDS             4483..5400
                     /gene="glyQ"
                     /locus_tag="VQU32_001918"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET2473316.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            5660..7696
                     /locus_tag="VQU32_001919"
     CDS             5660..7696
                     /locus_tag="VQU32_001919"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET2473317.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSELFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            7738..7995
                     /locus_tag="VQU32_001920"
     CDS             7738..7995
                     /locus_tag="VQU32_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HET2473318.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
BASE COUNT         2554 a         1431 c         1794 g         2328 t
ORIGIN      
        1 gctttctttt ccgaataaat agatagaagc atagaatcta gcaaacctag atttaaaaat
       61 gtgctataat agaaggagga aaaggaggat tctcagacat ccaggtatca gcccaactaa
      121 tgatttgtca atttatccgc gatatgctgg acttgccagc aaaaaatgtg acgattttgg
      181 agggaagtaa cattcacgtc ttgccttcca tgccctactc agcgcaagat ttctatacta
      241 gtatagacgt cttggcggag ttagataatg gaatccaagt tatcatcgaa attcaggttc
      301 atcatcagaa ttttttcatc aatcgcctat gggcttatct gtgcagtcag gttaatcaaa
      361 acctagaaaa aattcgccaa cgtgaaggtg atacccacca gagctacaaa caaatcgcac
      421 tagtatacgc tatcgcaatt gtcgatagta attacttctc agatgaccta gcttttcata
      481 gttttatagt aaaatgaaat gagaacagga caaatcgatc aggacagtca aatcgatttc
      541 taacaatgtt ttagaagtat aggtctacta ttctagcttc aatctactag aaattccata
      601 gatagaaaac tacataatct ctacagatac ggatgttgga gttgatgtaa gatgctttgg
      661 cttgctagag gaattgtgga ttgccaaatt gtatcattga aattattgct caaatttgtt
      721 atgatataaa tatgaataaa agtagactag gacgtggcag acacgggaaa acgagacatg
      781 tattattggc tttgattggt attttagcaa tttctatttg cctattaggc ggatttattg
      841 cttttaagat ctaccagcaa aaaagttttg agcaaaagat tgaatcgctc aaaaaagaga
      901 aagatgatca attgagtgag ggaaatcaga aggagcattt tcgtcagggg caagccgaag
      961 tgattgccta ttatcctctc caaggggaga aagtgatttc ctctgttagg gagctgataa
     1021 atcaagatgt taaggacaag ctagaaagta aggacaatct tgttttctac tatacagagc
     1081 aagaagagtc aggtttaaag ggagtcgtta atcgtaatgt gaccaaacaa atctatgatt
     1141 tagttgcttt taagattgaa gagactgaaa agaccagtct aggaaaggtt cacttaacag
     1201 aagatgggca accttttaca cttgaccaac tgttttcaga tgctagtaag gctaaggaac
     1261 agctgataaa agagttgacc tccttcatag aggataaaaa aatagatcaa gaccagagtg
     1321 agcagattgt aaaaaacttc tctgaccaag acttgtctgc atggaatttt gattacaagg
     1381 atagtcagat tatcctttat ccaagtcctg tggttgaaaa tttagaagag atagccttgc
     1441 cagtatctgc tttctttgat gttatccaat cttcgtactt actcgaaaaa gatgcggcct
     1501 tgtaccaatc ttactttgat aagaaacatc aaaaagttgt cgctctaacc tttgatgatg
     1561 gtccaaatcc agcaacgacc ccgcaggtat tagagaccct agctaaatat gatattaaag
     1621 cgactttctt tgtgcttggg aaaaatgttt ctgggaatga ggacttggtg aagaggataa
     1681 aatctgaagg tcatgttgtt ggaaaccata gttggagcca tccgattctc tcgcaactct
     1741 ctcttgatga agctaaaaag cagattactg atactgagga tgtgctaact aaagtgctgg
     1801 gttctagttc taaactcatg cgtccacctt atggtgctat tacagatgat attcgcaata
     1861 gcttggattt gagctttatc atgtgggatg tggatagtct ggactggaag agtaaaaatg
     1921 aagcatctat tttgacagaa attcagcatc aagtagctaa cggctctatc gttttgatgc
     1981 atgatattca cagtccgaca gtcaatgcct tgccaagggt cattgagtat ttgaaaaatc
     2041 aaggttatac ctttgtgacc ataccagaga tgctcaatac tcgcctaaaa gctcatgagc
     2101 tgtactatag tcgtgatgaa taagcaagaa aaaataggtc tgttagatat ttgacagact
     2161 tattttttac agaatatagt actacttaaa aaatgtttta tgctataatt gatgaataaa
     2221 atagaaggag aagcatatga atacctatca attaaataat ggagtagaaa ttccagtatt
     2281 gggatttgga acttttaagg ctaaggatgg agaagaagcc tatcgtgcag tgttagaagc
     2341 cttgaaggct ggttatcgtc atattgatac ggcggcgatt tatcagaatg aagaaagtgt
     2401 tggtcaagca atcaaagata gcggagttcc acgtgaagaa atgttcgtaa ctaccaagct
     2461 ttggaatagt cagcaaacct atgagcaaac tcgtcaagct ttggaaaaat ctatagaaaa
     2521 actgggcttg gattatttgg atttgtattt gattcattgg ccgaacccaa aaccgctcag
     2581 agaaaatgac gcatggaaaa ctcgcaatgc ggaagtttgg agagcgatgg aagacctcta
     2641 tcaagaaggg aaaatccgtg ctatcggcgt tagcaatttt cttccccatc atttggatgc
     2701 cttgcttgaa actgcaacta tcgttcctgc ggtcaatcaa gttcgcttgg cgccaggtgt
     2761 gtatcaagaa caagtcgtag cttactgtcg tgaaaaggga attttattgg aagcttgggg
     2821 gccttttggt cagggagaac tatttgatag caagcaagtg caagaaatcg cagcaaatca
     2881 cggaaaatcg attgctcaga tagccttggc ctggagcttg gcagaaggat ttttaccact
     2941 tccaaaatct gtcacaacct ctcgtattca agctaatctt gattgctttg gaattgaact
     3001 gagtcatgag gagagagaaa ccttaaaaac gattgctgtt caatcgggtg ctccacgagt
     3061 tgatgatgtg gatttctaga aaatcataaa aagaattgta cattattcta atttttgata
     3121 taatagtcag caggaaagaa agtcttatgg cgttcttcaa gcgagcttgg gatagtggga
     3181 gccaagtagg gcaaaataat gagctggcgc tttctgtagt attttcaaaa acaatgaagt
     3241 aataaattag ggtggaaccg cgtttctgac gcccctaggt taaatcaacc taggattgtc
     3301 ggacgcggtt cttttgctta ttcagtctat tgtgtgaaag aaaggagagc cgtggacaac
     3361 ctttatcttg taaaagacga tagtcaacta gctacatttc gtgattttgt agtaagaaat
     3421 actgaaaagt tgaaagatta tcaatctttt ttaaagaatg aacttgcagt ctgtgattta
     3481 ccgcaagccg ttatttggtc agattttaat gctgctacac agattattag ggaaagcgct
     3541 gttccagcct atacaaataa tagacgaatg gttatggcgc ctgatttagc tgtttggaaa
     3601 gagttgtatt tgtatcaatt gatggactac gagtgttctc agcaaactca agcaatagaa
     3661 agtcactatc attctttatc tgaaaatttc ctcttacaga ttgtaggaca tgagttagct
     3721 cattggtcgg agcatttttt agatgatttt gatggttatg actcttatat ctggttcgaa
     3781 gaggggatgg ttgaatatat tagtcgcaag tatttcttga cagaagagga atttcaagcg
     3841 gaaaaaattt gtaatcaatc tctcgtagaa ctttttcaga agaagtatgg ttggcattca
     3901 ttgaatgatt ttggttcttc gacttatgat aagaactatg caagtatttt ttatgaatac
     3961 tggcgcagct ttttgacagt agataagttg gtagaaaatt taggtagtgt acaagcggtc
     4021 ttagattctt atcatttatg ggcaaataca gaaaaaactt ttcccttgtt agattggttt
     4081 gttcagcaga aattaattga aaaagaaata taaaaactaa aggagtaaac aatgtctaag
     4141 aaattgacat ttcactgcgt cagtggcaga aacctcctta cagtcggact gccccacgct
     4201 cagcactaga gtgcctgagc tagacgcagt actaactcgt cttgcctcgt atgatcgacg
     4261 aggcagactc atgtcgcaag taattatttt ttattaagga gtaaacaatg tctaagaaat
     4321 taacatttca ctgcatcagt ggcagaaacc tccttacagt cggactgccc cacgctcagc
     4381 accctagtgc tgagctagac gcagtactaa ctcgtcttgc ctcgtataat cgacgaggca
     4441 gactcgtgtc gcaagaaatc attttttatt aaggagtaaa caatgtctaa gaaattaaca
     4501 tttcaagaaa ttattttgac tttgcaacaa ttttggaatg accaaggttg tatgcttatg
     4561 caggcttatg acaatgaaaa aggtgcgggg acaatgagtc cttacacttt ccttcgtgct
     4621 atcggacctg agccatggaa tgcagcttat gtagagccat cacgtcgtcc tgctgacggt
     4681 cgttatgggg aaaaccctaa ccgtctctac caacaccacc aattccaggt ggtcatgaag
     4741 ccttctccat caaatatcca agaactttac cttgagtctt tggaaaaatt gggaatcaat
     4801 cctttggagc acgatattcg ttttgttgag gacaactggg aaaacccatc aactggttca
     4861 gctggtcttg gttgggaagt ttggcttgac ggaatggaaa tcactcagtt cacttatttc
     4921 caacaagtcg gtggattggc aactggccct gtgactgcgg aagttaccta tggtttggag
     4981 cgcttggctt cttacattca agaagtagac tctgtctatg atatcgagtg ggctgatggt
     5041 gtaaaatacg gagaaatctt tatccagcct gagtatgagc actcaaaata ttcatttgaa
     5101 atttcggacc aagaaatgtt gcttgaaaac tttgataagt ttgaaaaaga agctggtcgt
     5161 gcattagaag aaggcttggt acaccctgcc tatgactatg ttctcaaatg ttcacatacc
     5221 tttaatctgc ttgacgcgcg tggtgccgta tctgtaacag agcgtgcagg ctatatcgct
     5281 cgtatccgta acttggcccg tgttgtagcc aaaacctttg ttgcagaacg caaacgccta
     5341 ggttacccac ttttggatga agaaacacga gttaaacttt tagcagaaga cgcagaatag
     5401 tgagtaatac tgtactctaa aatcgttaga ggcaagtcga agacttgcta gagggatatg
     5461 caccgccgta ctgttatgtg gggatttttg aaaccttagg ttcaaaaatc agtcagctaa
     5521 atccactttg atgagattgt tggaaatctt gataaattta gcattggatt tccatacaga
     5581 ctcacagagt tagttagcga cgtccccagt acctaaggtg catatcaaaa aagattgaag
     5641 aaaatgtaaa ggaaaaaaca tgacaaaaaa cttattagta gaactcggtc ttgaagaatt
     5701 accagcctat gttgtcacac caagtgaaaa acaactaggc gaaaaaatgg cagccttcct
     5761 caaggaaaac cgcctgtctt ttgaagccat tcaaaccttc tcaacaccac gtcgtttggc
     5821 tgttcgtgtg actggtttat cagacaaaca gtctgatttg acagaagatt tcaagggacc
     5881 agcaaagaaa attgccttgg atagtgatgg aaactttacc aaagcagctc aaggatttgt
     5941 ccgtggaaaa ggcttgactg ttgaagatat cgaattccgt gaaatcaagg gtgaagaata
     6001 tgtctatgtc actaaggaag aagttggtca agcagttgaa gccattgttc ctggtgttgt
     6061 agatgtcttg aagtcattga ctttccctgt cagcatgcac tgggctggaa atagctttga
     6121 atacattcgt cctgtccaca ctttaactgt tctcttggat gagcaagagt ttgacttgga
     6181 tttccttgat atcaagggaa gtcgtgtgag ccgtggccat cgtttcttgg ggaaagaaac
     6241 caaaattcag tcagcattga gctatgaaga agaccttcgt aagcagtttg taatcgcaga
     6301 tccatgtgaa cgtgagcaaa tgattgttga ccaaatcaag gaaattgagg caaaacatgg
     6361 tgtacgtatc gaaattgatg cggatttgct gaatgaagtc ttgaatttgg ttgaataccc
     6421 aactgccttc atgggaagtt ttgatgctaa ataccttgaa gttccagaag aagtcttggt
     6481 gacttctatg aaggaacacc agcgttactt tgttgttcgt gatcaagatg gaaaactctt
     6541 gccaaacttc atttctgttc gtaacggaaa cgcagagcgt ttgaaaaatg tcattaaagg
     6601 aaatgaaaaa gtcttggtag cccgcttgga agacggagaa ttcttctggc gtgaagacca
     6661 aaaattggtg atttcagatc ttgttgaaaa attaaacaat gtcaccttcc atgagaagat
     6721 tggttccctt cgtgaacaca tgattcgtac gggtcaaatc actgtacttt tggcagaaaa
     6781 agctggtttg tcagtggatg aaacagttga ccttgctcgt gcagcagcca tttacaagtt
     6841 tgacttattg acaggtatgg ttggtgaatt tgacgaactc caaggaatta tgggtgaaaa
     6901 atacaccctt cttgctggtg aaactccagc ggtggcagct gctattcgtg aacactacat
     6961 gcctacatca gctgaaggag aacttccaga gagcaaggtc ggcgcagttc tagccattgc
     7021 agacaaattg gatacgattt tgagtttctt ctcagtagga ttgattccat caggttctaa
     7081 tgacccttat gcccttcgtc gtgcaactca aggtgtggtt cgtatcttgg atgcctttgg
     7141 ttggcacatt gctatggatg agctgattga tagcctttat gcattgaaat ttgacagttt
     7201 gacttatgaa aataaagcag aggttatgga ctttatcaag gctcgtgttg ataagatgat
     7261 gggctctact ccaaaagata tcaaggaagc agttcttgca ggttcaaact ttgttgtggc
     7321 agatatgttg gaagcagcaa gtgctctcgt agaagtaagc aaggaagaag attttaaacc
     7381 atctgttgaa tcactttctc gtgcctttaa cctggctgag aaggcagaag gggttgctac
     7441 agttgattca gaactatttg agaatgacca agaaaaagct ttggcagaag cagtagaaac
     7501 actcgtttta tcaggacctg caagtcagca attgaaacaa ctctttgcgc ttagcccagt
     7561 cattgatgct ttctttgaaa atactatggt aatggctgaa gatcaggctg tccgtcaaaa
     7621 tcgtttggca atcttgtcac aactaaccaa gaaagcagct aagtttgctt gttttaacca
     7681 aattaacact aaataaaatt tgataaacgg actttatctt attacaaagg agaagaaatg
     7741 gatccgaaaa aaattgctcg tatcaatgag cttgctaaaa agaaaaaaac agaaggctta
     7801 acaccagaag aaaaagtgga acaagccaaa ctacgtgagg agtacatcga aggttatcgc
     7861 cgcgctgttc gtcaccacat tgaaggaatc aaaattgtgg acgaagaagg aaacgatgtt
     7921 acaccagaaa aactacgcca agtacaacgt gaaaaaggat tacatggccg tagtcttgat
     7981 gacccaaatt cataatatgt actctttctt ttaacaaaga tagatgaaac gataacaaag
     8041 agactagcag tttgtgtttg ctagtctttt ttcgctaaaa aaggaaccat aatggttcct
     8101 aaaaact
//
LOCUS       DAVBBS010000075         7893 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVBBS010000075 DAVBBS010000000
VERSION     DAVBBS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7893
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.075"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            101..1078
                     /locus_tag="VQU32_001921"
     CDS             101..1078
                     /locus_tag="VQU32_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2473319.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGVRFEAEK"
     gene            1075..2157
                     /locus_tag="VQU32_001922"
     CDS             1075..2157
                     /locus_tag="VQU32_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2473320.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFELLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(2221..3270)
                     /locus_tag="VQU32_001923"
                     /pseudo
     CDS             complement(2221..3270)
                     /locus_tag="VQU32_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(3654..4850)
                     /gene="tuf"
                     /locus_tag="VQU32_001924"
     CDS             complement(3654..4850)
                     /gene="tuf"
                     /locus_tag="VQU32_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET2473321.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(5761..6630)
                     /locus_tag="VQU32_001925"
     CDS             complement(5761..6630)
                     /locus_tag="VQU32_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET2473322.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(6853..7533)
                     /locus_tag="VQU32_001926"
     CDS             complement(6853..7533)
                     /locus_tag="VQU32_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2473323.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEKPEIPSPQEPGTPGEPTPEKPIPQPNPEHPSVPTPNPELPNQETPTPDK
                     PTPEPGTPKAETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAGLGLGF
                     FKKKEDEK"
     gene            complement(7491..>7893)
                     /locus_tag="VQU32_001927"
     CDS             complement(7491..>7893)
                     /locus_tag="VQU32_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HET2473324.1"
                     /translation="FKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANYYKE
                     GSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPANAY
                     QKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
BASE COUNT         2299 a         1646 c         1489 g         2459 t
ORIGIN      
        1 aaagctaata gcttatttga aattttctcc tctctgttgg atagtcccgt ctatctatgt
       61 tataatgaaa gaaggattta aaatcggaaa aggagtattt atgctaaaat taggtgtcat
      121 cggaactggc gctatcagcc atcatttcat agaagcagcc cataccagtg gagaatacca
      181 actggtcgca atctattcta gaaaactaga aactgcagca acctttgctt ctcgctatca
      241 gaatatccaa ctattcgatc aattagaagt cttcttcaaa tcttcttttg atttagtcta
      301 tatcgccagt ccaaactcct tacattttgc tcaggcaaaa gctgccttgt ctgctggtaa
      361 acatgttatt cttgaaaagc cagctgtcac tcaaccacaa gaatggtttg atttgattca
      421 aacagctgaa aaaaataact gttttatctt tgaagcagct cgtaattatc acgagaaagc
      481 ctttactact atcaaaaact ttttagcaga taagcaagtg ttaggagcag atttcaacta
      541 tgccaaatac tcttccaaga tgcctgactt attggctgga caaacaccaa atgtcttttc
      601 agaccgtttt gctggtggag ccctcatgga tttggggatt tatcccctct atgctgctgt
      661 tcgtcttttt gaaaaagcta atgacgcgac ttaccatgct caacagcttg acaatagcat
      721 tgacctaaat ggagatggta tcctcttcta cccagactat caagttcaca tcaaggctgg
      781 aaaaaacatc acttccaatc ttccttgcga gatttataca acagatggaa ccttgactct
      841 caacacgatt gagcatattc gctcagctat ttttaccgac caccaaggaa atcaagtcca
      901 gcttcctatc caacaggctc ctcatacgat gactgaggaa gtcgctgcat ttgcacacat
      961 gatccagcaa ccagatctga atctctacca aacttggctg tatgatgcag gctctgttca
     1021 tgagctacta tataccatgc gccagactgc tggcgttaga tttgaggcag aaaaatgaaa
     1081 accaaactac cgactgaatg gcaagaactg agtgaccaac tcggtttcca agaattcacc
     1141 cccattcaaa ctcaactatt tgagctcctt cttgctggag aaaacctcct aggagtgagc
     1201 caaaccggaa ctggtaagac cctagcgtat ctcctaccaa gtcttctcag actacaaaag
     1261 aaaaaagccc aacaactctt gattctagca ccaaacacgg aacttgctgg acagattttt
     1321 gatgtatgta aaacgtgggc agaagctatc ggattgactg ctcaactctt cctttcaggt
     1381 tcaagtcaga aacgacaaat tgaacgtctt aaaaaggggc cagaaattct gattggaact
     1441 cctggccgta tctttgaact cattaaactt aaaaaaatca agatgatgaa tgtggaaacc
     1501 atcatcctgg atgaatttga ccaattacta gatgattctc agattcactt tgtagagaaa
     1561 atcactcact acgcacctcg tgaccaccaa ctggtctaca tgagtgccac gaccaagttt
     1621 gaccaagaaa agattgtgcc gaatacacgc actattgatc tctctgatca aaaattggac
     1681 aatatccagc atttctacat gcaggtagac caacgtcatc gagtggatat gctacgaaaa
     1741 ctggctcatg tagaggattt ccgtggtcta gtcttcttca atagcctgtc agaccttggg
     1801 aatgccgagg aaaaactaca gtatcgtgat atattggctg tttccctcgc tagtgatgtt
     1861 aatgttaaat ttagaaaaat catcttagaa aagtttaaag ataaccaact aaccctgctt
     1921 cttgcaactg accttttggc tcgtggaatt gatatcgata gcctagaatg tgtcgtaaac
     1981 tttgatattc ctagagatag cgaaacctac acacaccgtg ctggccgtac tggtcgtatg
     2041 ggcaaggaag gttatgttat cactctcgtc actcatccag aagaaatcaa aaaacttaaa
     2101 aagtttgcaa gtatccgaga aattgtccta aaaaaccaag aactctatat caaataaagc
     2161 cccattgctc tatacacatc cagtgtatgg agctttttta taagcaattc aattttatac
     2221 tcatctgctt tcaaaaagca ttctagtcca tctccgatta acgatggact ttatcacctc
     2281 cttctccaat ccttgtatga catcttgaag ttgattcatg acatcttcca aaattcgaaa
     2341 ggctttattc ttaaatccac gtttacgaat ctctttccac acttgttcaa tggggttcat
     2401 ctctggtgtg tatggaggaa taaatgcaaa accaatatta gtcggaatct ttaagatact
     2461 tgatttatgc catatagcat tgtccataac gagtaaaaaa taatcatctg gataagcttg
     2521 tgaaagcttt tctaaaaagg cgttcatcca ctcagtatta catctaccag ctattaagaa
     2581 aaatgattcg cctgtatggg catcaacagc tccataacaa tagcgaaatt ctcgtatata
     2641 gtgactatgg atatgtggac ctactcctat tggagcccaa caagatccca gtttactgat
     2701 tctaccgaaa ccagcctcat cttggtacat caagcgaacc ttacgaaaac gtctactata
     2761 tttaaaacgc tttcttgtct tcttgaattg agattttatt tttagacgcg acaatggttt
     2821 gagcgtctgc tttcttagga tgttctggac gtggcataat atttcgccaa ccatggcgct
     2881 tcaacagttg atagaaggca tcacgtgtgt aggaacgacc taactccttt ttataagcct
     2941 gaaataaggc atcaattgta acaaattctc ctgcctctgc agccttcaaa tggcgggcaa
     3001 gaaagacttt ctcttcctca actgtcatat atgcatggtt acgaccacca cgtgtttctt
     3061 gaaggagaga gtcgagtccg aactcctcat atttttttac atttcgccaa atcattgttt
     3121 gattacagtc taaaagttct ataatctctt tataagattt gcccatatca gacgaaatag
     3181 aacgatttga agacgtttat gatatttagc tgtacgagag tcttttaaaa gtgttttgat
     3241 ggtttggatt tcttctttaa ttgatttcat attactattg tataatgctt tttgatttta
     3301 gtctagtatt atactcttcg aaaatctctt caaaccgcgt cagcttcgcc ttgtcgtata
     3361 gatgttactg acttcgtcag ttctatctac aacctaaaaa cagtgttttg agcagtctgt
     3421 agctaacttt ctatagtaaa atgaaataag aacaagacaa atcgatcagg acagtcaaat
     3481 cgatttctaa caatgtttta gaagtaaatg tgtactattc tagtttcaat atattatagt
     3541 ttgctctttg attttcattg agtattagtt caatatctaa aaaaagaacc ttgccaagca
     3601 agattctttt agtgtctgac ttaaataatt gttcttctgg gaactaaatc gaattaagct
     3661 tcgatttctg taaccatacc tgaaccaaca gtacgtccac cctcacggat agagaatgta
     3721 gtaccttgtt ctacggcgat tgggtgaatc aactcaacgt cgattgtcac gttatcacca
     3781 ggcattacca tttcagtacc tgctggaagt tcgattgaac ctgtaacgtc agtagtacgg
     3841 aagtagaatt gtggacggta gttgttgaag aatggagtgt gacgtccacc ttcttcttta
     3901 gtaaggatgt agacttcacc tttgaattta gtgtgtgggt tgattgaacc tggtttagcg
     3961 ataacttgtc cacgttcgat ttcatcacgt tgaacaccac gaagaaggac acctacgtta
     4021 tctccagcaa gaccttcgtc aagttgttta cggaacattt caacaccagt aacaactgct
     4081 ttttgagttt cttctttgat accaacgatt tcgatttcgt cgttgacttt aacgatacca
     4141 cggtcgatac gtcctgaagc aactgtacca cgtccagtga ttgagaatac gtcctcgact
     4201 ggaagaagca atggtttgtc agtgtcacgt tctggttctg ggatatactc atcaactgtg
     4261 ttcatcaatt ccataacgat gtcttcgtat ttagagtcac cttcaagagc tttaagtgct
     4321 gaaccttgga taactggaag atcgtcacct gggaagtcgt attctgacaa taggtcacgg
     4381 atttccattt caaccaattc aagcaattct tcgtcgtcaa ccaagtcaac tttgttcatg
     4441 aagacgataa ggtgtttaac accaacctga cgtgaaagaa ggatgtgctc acgagtttgt
     4501 ggcattggtc cgtcagttga agctactaca aggatagctc cgtccatttg agcagcacca
     4561 gtgatcatgt ttttaacgta gtccgcgtgt cctggagcgt cgatgtgagc gtagtgacgt
     4621 ttttcagttt cgtactcaac gtgcgcagtg ttgatagtga taccgcgttc gcgttcttct
     4681 ggagcagcat cgatagacgc atagtcttta ggttggttaa ctgatgaagg caagcgacgt
     4741 gccaaaacag ttgtgatagc tgcagttagg gtagttttac cgtggtcaac gtgtccgata
     4801 gtaccaatgt taacgtgtgg tttactacga tcgtattttt cttttgccat ttgagtaaaa
     4861 gcctccaata aaatatattt tatagataga cagtaggcaa tacagtctaa ctttccttac
     4921 tattttatca aatttcagct aaattgcaag tgttttacaa cgtttttgtg aattattctt
     4981 ggtaaaaatt taactaatta atagaaagta ctaaatagac ttatccttca aggagttttc
     5041 agcattatcc atccactcac ttgctgaaaa tgtaaacagt ctctggttta tatagtaaaa
     5101 tgaaacaaga atagagcaaa tcgatcagga cagtcaaatc gatttctaac aatgttttag
     5161 aagtagatgt gtactattct agtttcaatc tactatacaa cctcaaaact gtgttttgaa
     5221 caacctgcgg ctagcttctt agtttgccct ttgattttca ttgagtataa aattgcagat
     5281 aaaacgataa caatcatagg actcagcaga gtcaatgtag gtcgaagaat agccccccta
     5341 atcgcttatt ttatagcaaa atgaaataga aactgaacat tttgttgagg aaggtaaatc
     5401 aattcctcac aatgtttgag aaagaaggga ggactattcc aacttcaacc cactataaag
     5461 cagacttaca agatagggaa cccatctccg ataatatata gtagaatgaa aactgcacaa
     5521 tttgattgga gaattcaaac caatttctaa caatgtttta gaaacaaggg tctattattt
     5581 caactgcaac ctactatagc ccattgaagc tggaatagcc cactgagact tctaaaccac
     5641 tgctggagag gagtttgttt agagtttctt tcatttccct aaaaaatcaa aaagagagaa
     5701 ccagtctgat cctctcttac ttatagatgg tgctcactca aatgttaaag gagctatttc
     5761 ttagagataa aggaatttga atacagctac tgccgcaatt gctgctgcga taggtgctac
     5821 tactggtacc caagaatacc accattttga atcgccttta tgctcaccaa gaactgattt
     5881 tggaaggaaa gcatgaagga gacgtggtcc caagtcacgg gctgggttca aggcaggtcc
     5941 tgtaggtcct ccaagtgatg ttaccaaagc cataacgagg aatccaagtg ccaagtgagc
     6001 cacagaaagt cctgaagcag tgtgtggagc cacctgtgct ttaatagcca agtcagaaaa
     6061 atcaactgtt tgtcctgctt ctattgcctt ttgtttcatg aattgaagca cttcagcacc
     6121 aaagaagttt ttagtcaaac caagagctgc aaagaacaaa acaaatgaac caacaaactc
     6181 attaatcaaa ccattgacag ttgctgcata gcgactttct tttgtaccat ggtcaatact
     6241 tgaaatagtt gagaaagttc ccaagatgtt atttggtttt tcagttttca agtagaatgg
     6301 acggtatgtt gccacaacta aggcttggcc aaagatagcc cccaagactt gcgcgataat
     6361 gtaaggtacc acttgtgccc aagggaaaag accgctaact gcaagcccta gagtgaaagc
     6421 agggttgatg tgattcccag atacgttacc aaacatcaag gctgggatca taacccccat
     6481 accataacca acagcgatga cgatccagcc actttggtga cctttcgtac ctttaagttc
     6541 aacgttggca actgcaccat tcccaagaat gatcaaaatg gcagttccca aaaattcagt
     6601 ggcatacttc agtgcccatg tgaaatccat ttgctagatt ctccttaaaa ttttttagat
     6661 attccttatt ttatcaattt ttaccctatt tagcaactga tttctggaaa tatttttaaa
     6721 aaaataggat tcatagtttc ccagttcaac gtacttgtaa gcgtttttat tcttcacgaa
     6781 agacctcatt ctcaaaacca ggaaaaagcc cacatctgtg atcttttcct ggttcctaga
     6841 ttctaaaatc tattattttt catcttcttt tttcttgaag aatcctaatc ctagaccagc
     6901 taacaaggtc atgataccag cactagccaa ggtagcatta gcttctgtac ctgtgtttgg
     6961 caattcctct ctcttacctg tctcataagt cggaacttct gggtctggat tcactggagt
     7021 ttcagctttt ggagtacctg gttctggagt tggtttatct ggtgttggag tctcttgatt
     7081 tggtagttct gggtttggtg tcggaacact tggatgctct ggatttggtt gtgggattgg
     7141 tttttctgga gtcggttcac ctggagtacc tggttcctga ggacttggaa tttctggttt
     7201 ttctggagtt ggtttatctg gtgttgataa acggtcatac cttaccgtta tttctttatc
     7261 actagagtct gacgtaactt cttgtgattc aactgttgga atatctggat ctttgtactt
     7321 gtcaatctta ccagatataa cctcgtccca gtttcctgtt gtccattcac cgtaggttac
     7381 aactcccgtg accttgttct cagtttttgt acggcttaag gttacaggtt gaacaacatc
     7441 ttcttttaca ttttggttcg taactttatc aacgtaatga atgatacgcg ttatagtctt
     7501 cgtctcagta gaggttgctg ttttgggaac cactgtttcc tcaacattct cacggtagta
     7561 atagtcaact gttgcaccgt cttctggtac gcatttgcag gagttgctac caaggtgtat
     7621 gttgttttcc ttgtgataac tcggtcttct ttgtcctcag ttgttgtttt cccttcaata
     7681 gtttttgatt ctgtggtata ctcagaacct atcgctaaat cagcttttat aacagactct
     7741 gccaacttct cttggctacc ttctttatag taattggctg ttactgtagc agtggttggc
     7801 gcttcgcttt ctctataaac taaggtcact gttctacctt cgcttacaat attcccagtt
     7861 aaacttgcag aatttgtatc tgcttcttta aaa
//
LOCUS       DAVBBS010000076         7652 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVBBS010000076 DAVBBS010000000
VERSION     DAVBBS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7652
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.076"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..1038
                     /locus_tag="VQU32_001928"
     CDS             <1..1038
                     /locus_tag="VQU32_001928"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit I is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HET2473325.1"
                     /translation="IKVMDERFGHSIYIYESETLSDNQDEIPIKLTNHSLIEPFELLT
                     EMYALPKYYEKDPTPVLAPFYFTFFGMMVADLGYGLLLFLGTMLALKIFHLPSETKRF
                     LKFFNILGVAVAIWGGIYGSFFGYELPFHLISTTSDVMIILVVSVVFGFITVFAGLLA
                     SGLQKVRMKKYAEAYNSGFVWCVILLGLLFIAVGMLMPDMRLLFVLGKWVSIFNAVGI
                     LVVSIIQAKSLSGIGAGLFNLYNISSYIGDLVSFTRLMALGLSGASIASAFNLIVGLF
                     PGTLAKLTIGLVLFILLHAINIFLSLLSGYVHGARLIFVEFFGKFYEGGGKPFQPLKA
                     SEKYIKVITKN"
     gene            1054..1530
                     /locus_tag="VQU32_001929"
     CDS             1054..1530
                     /locus_tag="VQU32_001929"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HET2473326.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            1564..2145
                     /locus_tag="VQU32_001930"
     CDS             1564..2145
                     /locus_tag="VQU32_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473327.1"
                     /translation="MSNLENLRESVIEQAHEKGRMKLLDSKKKIDDEFEMQKSVIVKK
                     KEVEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALIEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNHLFSEQPISNESGLLI
                     SIGKIDDNYLYKTLIGSISKEESSSIANQIFIN"
     gene            2210..3217
                     /locus_tag="VQU32_001931"
     CDS             2210..3217
                     /locus_tag="VQU32_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HET2473328.1"
                     /translation="MDTNLFSKINTTISVKENDFITEDKFQKIIQSKDTETMAFILEA
                     TPYHLSIDVLENPSQTETSLMTKLVNDYRWAYAESPSNKIVTLFALRYVYHNIKVLLK
                     SKAAIKKDFSKLLIPIGIFDIESLKHLVSSLHSDTLPDFMVHEVESIWNEYETFNNIR
                     VLDVGADLAYFKHLKLLSNELDEVLSQVIVAMIDFYNIITVKRGLSQNKSHGDILQLL
                     SDEGSISAKEFIYIVENQEIFVWFNKINPSLDSIFSTYELKMQDATISSSELEFLCDL
                     LLYKILDQGRYNVDGPLVLARYLLGCEFEVKNLRMIISALQNTIPFGSIKERIRPHYG
                     S"
     gene            3207..3527
                     /locus_tag="VQU32_001932"
     CDS             3207..3527
                     /locus_tag="VQU32_001932"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HET2473329.1"
                     /translation="MEANKYKIGIIGSRDIILPFSMIGFDIFPAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            3590..5365
                     /locus_tag="VQU32_001933"
     CDS             3590..5365
                     /locus_tag="VQU32_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HET2473330.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFSNVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGIPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGIYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            5366..6751
                     /locus_tag="VQU32_001934"
     CDS             5366..6751
                     /locus_tag="VQU32_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HET2473331.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLSSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            6774..7385
                     /locus_tag="VQU32_001935"
     CDS             6774..7385
                     /locus_tag="VQU32_001935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HET2473332.1"
                     /translation="MVRLNVKPTRMELNNLKERLKTAERGHKLLKDKRDELMRRFISL
                     IRENNRLRKEVESYLIDNLKSFAVAKSLKNSLMVEELFSIPSKEIELFIEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIDKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
     gene            complement(7496..>7652)
                     /locus_tag="VQU32_001936"
     CDS             complement(7496..>7652)
                     /locus_tag="VQU32_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HET2473333.1"
                     /translation="YSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERT
                     KFVLSRA"
BASE COUNT         2508 a         1067 c         1559 g         2518 t
ORIGIN      
        1 ataaaagtta tggatgagcg ttttggacat tctatttata tttatgaatc ggaaacattg
       61 tcggataatc aagatgaaat acctatcaaa ttaacgaatc attctttaat tgaaccattt
      121 gaattattga cagaaatgta tgctctgccc aaatattatg agaaagatcc tacacctgta
      181 ttagcaccat tttactttac attttttgga atgatggttg ctgatttagg ctatggttta
      241 ctattgtttt taggaacaat gttagcatta aaaatttttc atctaccttc agaaactaag
      301 agatttttaa aattctttaa tatattaggg gtagccgttg caatttgggg tggaatctat
      361 ggctcatttt ttggatatga gttgccattt catctgatat ctacaacctc tgatgtcatg
      421 attatattag tagtgtcagt tgtgtttggg tttattacag tatttgcagg tttgttagct
      481 tcaggactac aaaaagtaag aatgaagaaa tatgcagaag catataattc aggatttgtg
      541 tggtgtgtta ttctgcttgg cttgttattt attgctgtcg gaatgttgat gcctgatatg
      601 agactgttat ttgtattagg gaaatgggta tctattttta atgctgtggg aattttggtt
      661 gtttctatta ttcaagccaa aagcttgtca ggtattggag caggattgtt taatctatat
      721 aacatttcat cttatatagg tgatttagtt agtttcactc gattgatggc attaggatta
      781 tctggagcaa gtatagcatc agctttcaat ttaattgttg gtttgtttcc gggaacattg
      841 gctaaactaa caattggatt agtattattc attcttttac atgcgatcaa tatttttcta
      901 tcgttactat caggatatgt tcatggagca cgtctgatat ttgttgaatt ttttggtaag
      961 ttttatgagg gtggaggaaa accatttcaa cctttgaagg cttctgagaa atatattaag
     1021 gttattacaa agaattaatg gaggatatat ataatggaac atttagcaac ttatttttca
     1081 acctatggag gagctttctt cgctgcattg ggaattgtat tggcggttgg attaagtggt
     1141 atggggtctg cttatggagt tggtaaggct gggcaatctg ccgcagcttt actgaaagaa
     1201 cagccagaaa agtttgcctc tgctttgata ttgcaattac tgcccggaac acaaggatta
     1261 tatggttttg ttattggaat tttgatttgg ttgcaattaa ctccagaact tcctttagaa
     1321 aaaggcgttg cttatttctt tgtagctctt ccaattgcta ttgtaggata cttttcggct
     1381 aagcatcaag gaaatgtagc agtagcggga atgcaaatct tggctaaaag accaaaagaa
     1441 ttcatgaagg gagcaatttt ggctgccatg gtagaaacct atgcaattct tgcctttgtc
     1501 gtatcattca ttttgaccct tcgtgtataa gaaattaatt tgctattcaa aggaggtgtc
     1561 taaatgagca atttagaaaa cttacgagag tctgttattg aacaagctca tgaaaaaggg
     1621 cgtatgaaat tattggattc caaaaagaag attgatgatg aatttgaaat gcaaaagtcg
     1681 gtcattgtaa agaaaaaaga agtggaacat gaacgaaagt taaaagaatt acaacagaaa
     1741 tatcaaataa tttttcaaca attaaaaaat aaggaacgcc aatcaacgtt agtatcaaaa
     1801 cagaaaatat taaaagaact ttttcaatct gctttaatag aaatggaatc ttggagtgca
     1861 gataaagaaa tggagttcat ctatcgaatt ctggaacgat attcacaaca agaggtcata
     1921 gtaacttttg gggagcggac tttagctaaa ttcaatctgg aacaattaga gaaattgaaa
     1981 ttctcttttc caaatcattt atttagtgaa cagcctatct caaatgaatc aggcttactt
     2041 atttcaatag gtaaaattga tgataactat ttgtataaaa cattaattgg atcgatttct
     2101 aaggaagaaa gttcaagtat cgcaaatcaa atttttatca attaaggatg aaattggtta
     2161 atccttctta gaaatttgga gtattccaat aaaattagaa aggtatttta tggatactaa
     2221 tcttttttca aaaataaata cgacgatttc ggtaaaagaa aacgatttta ttacagaaga
     2281 taaatttcaa aaaattatac aatccaaaga tacggagaca atggcattta tcttagaagc
     2341 aactccctat catttatcga ttgacgtttt agaaaatcct agtcagacag agacttcgct
     2401 aatgacaaaa ttagtcaatg attatagatg ggcctatgct gaaagtccgt ctaataaaat
     2461 tgtgacttta tttgctttac gatatgttta tcataatatc aaagttttat taaaatctaa
     2521 ggcggcaatt aagaaagatt tttctaaatt attaattcca atagggattt ttgatataga
     2581 aagtttaaaa catttagttt cttccttaca ttcagataca cttcctgatt ttatggttca
     2641 tgaagtagaa tcaatttgga atgagtatga aacttttaat aatattcgtg tacttgatgt
     2701 cggagctgat ctagcatatt ttaaacatct gaaactttta tctaatgagt tagatgaggt
     2761 gctgtctcag gttattgtcg caatgattga cttttataat attattactg taaaacgtgg
     2821 tttatctcaa aataagagtc atggggatat tttacaatta ctttcagatg aaggaagtat
     2881 ttctgctaaa gaatttatat acattgtaga aaatcaagaa atatttgtgt ggttcaataa
     2941 aataaatcca agcttagatt caatcttttc aacttatgaa ttgaagatgc aggacgcaac
     3001 aatttcatct tctgagttag aatttttatg tgatttatta ttgtataaaa ttttagatca
     3061 aggaaggtac aatgtagatg ggccgttagt acttgctaga tatttattgg gatgtgagtt
     3121 tgaagtaaag aatctcagaa tgattatatc agctcttcaa aatacaattc cctttggatc
     3181 aataaaagaa aggatacgcc cgcattatgg aagctaataa gtataaaatt ggcataattg
     3241 gtagccgtga tattatttta ccctttagca tgattgggtt tgatatattt cctgcctatc
     3301 aagaacaaga agctataaat actctaagaa aattagccca atctgattat ggtgtcattt
     3361 atatcactga agacattgct tcaatgatat tagatacaat tcgccattat gattctcaag
     3421 ttgtgcctgc tattatttta ttaccgactc ataaacaagg tttaaattta ggattaaaac
     3481 gtatagagga taatgtggag aaagcagtag gacacaatat tttataataa tgtacaaaat
     3541 tgtctgtaat attattctat aatttttgga cttagtaagg agaataactt tgactcaagg
     3601 gaagattata aaagtatcgg gacctctagt tattgcatca ggtatgcagg aggctaatat
     3661 tcaagatatt tgccgtgtag gtaagctagg gttaatcggt gaaattattg aaatgagaag
     3721 agatcaggca tctatccaag tctatgagga aacatctggt cttggtccgg gagaacctgt
     3781 tgttacaact ggagaacctc tctcggttga attaggacca ggattgattt ctcaaatgtt
     3841 tgatggcata caacgcccat tagatcgatt taaattggct actcataatg attttctagt
     3901 tcgtggggta gaagttccaa gtttggatag agatattaag tggcattttg attccactat
     3961 agcaattggt caaaaagtga gtacgggtga tattctagga actgtcaagg aaaccgaggt
     4021 agttaatcat aaaattatgg ttccttatgg agtatctggc gaagtcgttt ctatcgcatc
     4081 tggcgatttt acaattgatg aagttgtata tgaaataaaa aaattggacg gtagtttcta
     4141 taaaggaacg cttatgcaaa aatggcctgt tcgcaaggcg cgtcctgttt ctaaacgttt
     4201 aatcccagaa gaaccattaa tcacaggtca acgagttatt gatacattct ttccagtaac
     4261 caaaggggga gcagcagctg ttcctggacc gtttggagca ggaaagacag ttgtacaaca
     4321 ccaagtagct aaattttcca atgttgatat tgttatttat gtcggttgtg gagaacgtgg
     4381 aaatgaaatg acggatgtac tgaatgagtt tcctgagttg attgacccta ataccggaca
     4441 atcaattatg caacggacag ttctgattgc taatacttca aatatgcctg ttgctgctcg
     4501 tgaggcttca atttatacag gaattaccat ggctgagtat tttcgtgata tgggctactc
     4561 tgtcgccatt atggctgatt cgacttcacg ttgggcagaa gcactacgtg aaatgtcagg
     4621 acgtctagaa gaaatgcctg gtgatgaggg ttatcctgct tatctgggaa gtcgtatcgc
     4681 tgaatattat gaaagagcgg gacgttctca ggttctaggg attccagaac gtgaaggaac
     4741 tattactgct attggagctg tatcgccacc cggtggagat atttcagaac cagttactca
     4801 aaacacttta cggattgtga aagttttttg ggggcttgat gctccgttgg cacagagacg
     4861 tcattttcct gcaattaact ggcttacatc ttattcactc tataaagaca gtgtgggaat
     4921 ttatatagat ggtaaagaga agacagattg gaatagtaaa ataactcgtg cgatgaacta
     4981 cttacaacgg gaatctagtt tagaggaaat tgttcgtctt gttggaattg attctctgtc
     5041 tgataatgaa cgactaacga tggaaattgc taaacaaatt cgagaagatt atttgcaaca
     5101 gaatgctttt gattcagtag atacattcac ttcgtttgca aaacaagaag caatgctaag
     5161 taatatccta acttttgctg atcaggcaaa tcatgcttta gagttaggtt cttactttac
     5221 agagattatg gaaggtaccg tggcagttcg agaccgtatg gcgcgaagta aatatgtttc
     5281 agaagataga ttagatgaaa tcaaaattat ttcaaatgag attacacatc aaattcattt
     5341 gatattagaa acaggaggtc tataaatgag tgttataaaa gaatacagaa ctgctagtga
     5401 agttgttggg cctcttatga ttgttgaaca agtaaataat gtgtcttaca atgagttagt
     5461 tgaaattcaa cttcataatg gagaaattcg tcgtggacaa gttttagaga ttcacgaaga
     5521 taaagcaatg gttcaacttt ttgaaggatc tagtggaata aatttagaaa agtctaaaat
     5581 tcgttttgct ggtcatgcac tagaattggc tgtatctgag gatatggttg gtcgtatttt
     5641 taatgggatg ggaaaaccaa ttgatggtgg accagattta attccagaga aatatttaga
     5701 tattgatggt caagctatta atcctgtatc tagagattat ccagatgaat ttattcagac
     5761 agggatctcc tctattgatc atttgaatac tcttgtacgt ggtcaaaaat taccagtatt
     5821 ttcaggttcg ggcttacctc ataatgaatt agcagctcag atagcaagac aagcgactgt
     5881 tttaaattct gatgaaaatt ttgcggttgt atttgcagca atgggtatta cttttgaaga
     5941 agctgagttt tttatggaag aactcagaaa aacaggagcg atcgatcgtt cagtcttatt
     6001 tatgaacttg gcaaatgatc ctgcaattga gcgtattgca actccccgca ttgctttaac
     6061 cgcggcagag tatctagctt ttgaaaaaga tatgcacgtt ctagttatca tgacggatat
     6121 gactaactat tgtgaagcgt tacgtgaagt ctcggcagcg cgccgtgaag ttccaggtag
     6181 acgaggctat ccgggatatt tatatacaaa tttatcaact ctatatgaaa gggctggtcg
     6241 cttagttggt aaaaaaggtt cagtgacaca gattcctatt ttaacaatgc cagaagatga
     6301 cataacacat ccaattcctg atttaactgg atacattact gaagggcaaa ttattttgtc
     6361 tcatgagttg tataatcaag gttatcgtcc accaatcaat gttttatctt ctctctctcg
     6421 attaaaagat aagggatctg gagaaggtaa aactcgtgga gatcatgctc caactatgaa
     6481 tcaactgttt gcagcctatg cccaagggaa aaaggttgaa gagttagcag tagtattagg
     6541 agaatcggct ttatctgatg tagataaatt gtatgtgaga tttacaaagc gttttgaaga
     6601 agagtacatc aaccaaggat tttataaaaa tcgaaatata gaagatacgt tgaatcttgg
     6661 gtgggaatta ctatcaattc ttcctagaac agagttaaaa cgtatcaaag atgatttgct
     6721 tgataaatat ttacctttgg tagaagttta atccagaaat ggagtgatta tctatggtac
     6781 gtttgaatgt aaaaccaact cgtatggaat tgaataactt aaaggaacgt ttgaaaacag
     6841 ctgaacgtgg acataagtta ttaaaggata aaagagatga attgatgagg cgatttattt
     6901 ctttgattcg tgagaataat cgacttcgga aagaagtaga aagttatcta attgataatc
     6961 taaaatcttt tgcagttgct aaatcattaa agaactctct aatggtggag gaattatttt
     7021 caattccatc gaaagaaatt gaattattta ttgagaaaga aaatatcatg agtgtaacag
     7081 ttcctagaat gcatatgaat attacttctc aaaatgagaa cagtgaatac agctatttat
     7141 cttctaatag tgaaatggat gatgtatttg ctacaatgaa tagtttaatt gataaattac
     7201 taagactggc agaagttgaa aaaacgtgtc agttaatggc tgatgaaata gaaaaaacac
     7261 gtagacgtgt aaatggttta gaatattcga ttattccaaa cttgtcggaa actattcatt
     7321 atatagaatt gaaactagag gaggcagaaa gagccaattt agttcgtatt atgaaagtga
     7381 agtagttcct ttatttagat ttttaattag atgaacaaat atcagcttgg ataaggcggt
     7441 aagcctttct aagctttttt aggctctttg tcaactgtag tgggttgaaa aaaagctaag
     7501 ctcgagaaag gacaaatttc gtcctttctt ttttgatatt cagggcgata aaaatccgtt
     7561 ttttgaagtt ttcaaagttc cgaaaaccaa aggcattgcg cttgataagt ttgatgagat
     7621 tattggtcgc ttccaatttg gcgttagaat ag
//
LOCUS       DAVBBS010000077         7474 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVBBS010000077 DAVBBS010000000
VERSION     DAVBBS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7474
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.077"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            60..929
                     /locus_tag="VQU32_001937"
     CDS             60..929
                     /locus_tag="VQU32_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET2473334.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            933..1889
                     /locus_tag="VQU32_001938"
     CDS             933..1889
                     /locus_tag="VQU32_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET2473335.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            1889..2653
                     /locus_tag="VQU32_001939"
     CDS             1889..2653
                     /locus_tag="VQU32_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2473336.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            2653..3363
                     /locus_tag="VQU32_001940"
     CDS             2653..3363
                     /locus_tag="VQU32_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2473337.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKIPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            3828..4484
                     /locus_tag="VQU32_001941"
     CDS             3828..4484
                     /locus_tag="VQU32_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2473338.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDESIDLPALREKFEANGIQVEEIARTSAKVL
                     "
     gene            4592..5687
                     /gene="prfB"
                     /locus_tag="VQU32_001942"
     CDS             join(4592..4663,4665..5687)
                     /gene="prfB"
                     /locus_tag="VQU32_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET2473339.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            5705..6397
                     /gene="ftsE"
                     /locus_tag="VQU32_001943"
     CDS             5705..6397
                     /gene="ftsE"
                     /locus_tag="VQU32_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HET2473340.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            6390..7316
                     /locus_tag="VQU32_001944"
     CDS             6390..7316
                     /locus_tag="VQU32_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2473341.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
BASE COUNT         2208 a         1292 c         1682 g         2292 t
ORIGIN      
        1 agaacctatc aaaaagtgag ggaaaaccct cggaattata aatagaaaga gtgaatctta
       61 tgctccaaca actcgtaaat ggtttgattc taggtagtgt ttacgcgctg ttagccctag
      121 gatataccat ggtttacgga attatcaagc tcatcaactt cgcccatggt gatatttata
      181 tgatgggagc ctttatcggt tatttcttga tcaattcttt ccaaatgaat ttctttgtag
      241 cgcttattgt agctatgcta gcgacagcta ttcttggtgt cgtgattgag tttcttgctt
      301 accgaccttt gcgccactct actcgtattg ctgttttgat tacggctatt ggggtttctt
      361 tcctattgga gtatggaatg gtctatctgg ttggtgccaa tacccgtgcc ttccctcaag
      421 cgattcaaac agttcgatat gatttgggac caattagctt aacaaatgtg cagttaatga
      481 ttttggccat ttccttgatt ttgatgattt tgttacaagt cattgtccaa aagactaaga
      541 tggggaaagc catgcgtgca gtatcagtag atagcgacgc ggcgcaattg atggggatca
      601 atgtaaaccg tacgattagc tttaccttcg ctttgggttc tgctcttgcg ggtgcggctg
      661 gtgttctgat tgctctttat tataactctc ttgagccttt gatgggggtt actccaggtc
      721 ttaaatcttt cgttgccgca gtacttggtg gtatcggaat tattcctggt gcggctcttg
      781 gtggctttgt gattggtcta ttggaaacct ttgcgactgc ctttgggatg tcagatttcc
      841 gtgatgccat tgtttatgga atcttgttgt tgatcttgat tgtccgccca gctggtatcc
      901 ttggtaagaa tgtgaaagag aaggtgtaaa cgatgaagga aaatttaaaa gttaatattc
      961 tatggttact ccttttgtta gctggctata gcttgattag tgtactggtt tcagtcggag
     1021 tacttaatct attctatgta cagattttac aacaaattgg aattaatatt attttggctg
     1081 ttggtctcaa cttaatcgtt ggtttttcag gacaattttc acttggtcat gctggtttca
     1141 tggcgattgg tgcctatgca gcagctatta ttggttctaa attaccaacc tacggtgcct
     1201 tctttggagc tatgcttgta ggggctttgc tttcaggagc agttgcctta cttgtcggca
     1261 ttccaacctt gcgcttgaag ggggactatc ttgcggtagc aactctaggt gtttctgaaa
     1321 ttatccgtat ctttatcatc aatggtggaa gtcttacaaa tggtgcggca ggtatcttag
     1381 ggattcctaa ctttacaact tggcaaatgg tttacttctt tgtcgtgatt acaaccattg
     1441 caaccttgaa cttcttgcgt agcccaattg gccgttcaac cctctctgtt cgtgaagatg
     1501 aaatcgctgc tgagtcagtt ggggttaata cgactaaaat taaaatcatc gcttttgtct
     1561 ttggtgccat tactgcaagt attgctgggt cacttcaggc aggatttatc gggtctgttg
     1621 taccgaaaga ttacaccttc attaactcaa tcaacgtttt gattattgtt gtatttggtg
     1681 gactcggttc cattacaggt gcgattgttt cggctattgt tctgggaatt ttgaatatgc
     1741 ttctccaaga tgttgctagt gtgcgtatga ttatttacgc tttagccttg gtattggtaa
     1801 tgattttcag accaggtgga ctccttggaa cgtgggaact gagcctatca cgtttcttta
     1861 aaaaatctaa gaaggaggaa caaaactaat ggcattactt gaagtaaaac agttaaccaa
     1921 acattttggt ggtctaacag ctgttggaga tgtgactctt gaattgaacg aaggggaact
     1981 ggttggatta atcggtccaa acggagctgg gaaaaccacc cttttcaacc ttttgaccgg
     2041 tgtttatgaa ccaagcgagg gaacagtaac cctagatggt caccttttga atgggaaatc
     2101 accttataag attgcctctt tgggacttgg acgtactttc caaaatatcc gtctctttaa
     2161 agatttaaca gttttagata atgttttgat tgcttttgga aaccatcaca aacagcatgt
     2221 ttttactagt ttcttacgct taccagcttt ttacaagagt gaaaaagaat taaaggctaa
     2281 agctttggaa ttgttgaaaa tctttgattt agatggtgat gcagagactc ttgctaaaaa
     2341 tctttcctac ggacaacaac gtcgtttgga aattgttcgt gcccttgcta cggaacctaa
     2401 aattctcttc ttagatgaac cagcagcagg tatgaaccca caggaaacag ccgaattgac
     2461 tgagttaatt cgtcgtatca aagatgaatt taagattaca atcatgttga ttgaacacga
     2521 tatgaatctg gtcatggaag taacagaacg tatctacgta cttgaatatg gccgtttaat
     2581 tgctcaagga actccagacg aaattaagac caataaacgc gttatcgaag cttatctagg
     2641 aggtgaagcc taatgtctat gttaaaagtt gaaaatcttt ctgtgcatta cggtatgatc
     2701 caagcagtcc gtgatgtaag ctttgaagtt aatgaaggag aagttgtttc ccttatcggt
     2761 gccaacggtg caggtaagac aactattctt cgcaccttgt caggtttggt tcgaccaagt
     2821 tcaggaaaga ttgaattttt aggtcaagaa atccaaaaaa taccagctca gaaaatcgtg
     2881 gcaggtggtc tttcacaagt tccagaagga cgccacgtct ttcctggctt gactgttatg
     2941 gaaaatcttg aaatgggagc tttcttaaag aaaaatcgtg aagaaaatca agctaacttg
     3001 aagaaggttt tctcacgctt tcctcgtctt gaagaacgta agaaccaaga tgcagctact
     3061 ctttcaggag gggaacaaca aatgcttgcc atgggacgcg ctcttatgtc aacaccaaaa
     3121 cttcttcttt tagatgaacc atcaatggga cttgccccaa tcttcatcca agagattttt
     3181 gatatcattc aagatattca gaagcaagga acaaccgtcc tcttgattga acaaaatgcc
     3241 aataaagcac ttgcaatctc tgaccgagga tatgtattgg aaacaggaaa aatcgtccta
     3301 tcaggaacag gaaaagaact cgcttcatca gaagaagtca gaaaagcata tctaggtggc
     3361 taaaacaatc cagtggattg ttttagtcgg cagatgaaga ttacgaagta atcatcaata
     3421 tagtccgggg gcccttttta gtcggtagat tgagattgcg aagcaatcat caaatccaga
     3481 ggattgtttt agccggcaga taaagattac gaagtaatca tcaatatagt ccgggggccc
     3541 tttttagtcg gtggatgaag attgcgaagc aatcatcaaa tccagaggat tgttttagcc
     3601 ggtagattga gattgcaaac aaatctgcat ctacattgaa agcttaattt ctaataattg
     3661 aaaaaatcga atgaaaagtt tcttaccttc attcacagag ctcgatttta gagctctttt
     3721 tgctagctta ttcatacttt tctaaatttt gaaaaagaaa tgtaagcgtt tgatagattt
     3781 gcaaaaagat tgtataatag ggataagaat agaaaaggag aagtctcatg gcagttaaag
     3841 attttatgac ccgcaaggta gtttatatta gtccagatac aacagtatct catgcagcag
     3901 atttgatgag agagcaaggt ttgcaccgtc tgcctgttat cgaaaatgat caattggttg
     3961 gtttggtgac tgagggaacc attgcacaag caagtccatc taaagcaaca agtctttcta
     4021 tctatgagat gaattatctt ctgaataaga caaaagtaaa agatgtcatg attcgcgatg
     4081 ttgtcactgt ctcaggctat gctagtctag aagatgcaac ttatctgatg ttgaaaaata
     4141 agattggtat tctccctgtc gtagataacc atcaagtata cggagttatt actgaccgtg
     4201 acgttttcca agcctttctt gaaattgcag gttatggcga agaagggatt cgtgtacgct
     4261 ttgttacaga agatgaagtt ggtgttcttg gaaaaattgt ttctttgatt gtagaagaaa
     4321 atttgaatat ctcccataca gtcaatattc cgcgtaagga tggtaaggtg attatcgaag
     4381 tgcaaatcga tgaatcaatt gatttaccag ccttgagaga aaaatttgaa gcaaatggta
     4441 ttcaagtgga agaaatcgct cgcacttcag caaaagtctt gtaagaaggg aagtccaaag
     4501 gcttcttttt tcatgaaaag gggattagag caaaagatgg aaagaaatga taaaatatgc
     4561 tataatgaaa taatgtaaaa aaggagtatt tatggacatt tcagtaattc gtcagaaaat
     4621 tgacgcaaat cgtgaaaaat tagcttcttt cagggggtct ctttgacctc gaagggctag
     4681 aggaagagat tgccatcttg gaaaacaaga tgacagaacc tgatttttgg aacgataata
     4741 ttgcggccca aaaaacgtcg caagaattga atgaattaaa aaacacttac aataccttcc
     4801 ataagatgga agagttgcag gatgaagtcg aaattttatt ggattttttg gctgaagacg
     4861 agtcagtgca tgatgaactg gtagcgcagt tagccgaact tgataagata atgaccagct
     4921 acgagatgac tctactcttg tcagaacctt atgaccacaa caatgccatc ttggaaatcc
     4981 atccaggttc tggtggtact gaggcgcagg actggggtga tatgttgctt cgtatgtata
     5041 ctcgttatgg taatgctaaa ggctttaaag tggaagtgtt ggattaccaa gcaggtgatg
     5101 aggctggtat taagtcggta actttatcat ttgaagggcc taatgcctat ggtctcctca
     5161 agtcagaaat gggtgttcac cgcttagtgc gaatctcacc atttgactct gccaaacgtc
     5221 gccatacctc tttcacatct gtagaagtga tgccagaatt ggatgatact attgaagtgg
     5281 aaatccgtga agatgatatc aagatggata ccttccgttc aggtggtgcc ggtggacaaa
     5341 acgtcaataa ggtttcaaca ggtgtacgtt taacccacat tccaactgga attgttgtcc
     5401 aatcaacagt ggatcgtacc cagtatggaa atagagatcg tgccatgaag atgttgcagg
     5461 ctaagctcta tcaaatggag caagataaga aggctgcgga ggtagattct ctcaaaggtg
     5521 agaaaaagga gatcacttgg ggaagccaaa tccgttctta tgtcttcacg ccttatacta
     5581 tggtaaaaga tcaccgaact agctttgagg ttgctcaggt agataaggtt atggatgggg
     5641 acctagatgg ttttatagat gcttatctca agtggcgaat tagctaagat agaaaggaac
     5701 tcacatgtca attattgaaa tgagagatgt cgttaaaaaa tacgacaacg gaacgactgc
     5761 tctacgcggt gtttcggtca gcgttcaacc gggggaattt gcttacatcg taggaccttc
     5821 aggagcaggg aagtcaactt ttattcgttc tctgtatcgt gaagtaaaaa tcgataaagg
     5881 aagtctatca gttgctggtt ttaatctggt taagatcaaa aagaaagatg tcccgcttct
     5941 acgtcgtagt gttggggttg tcttccaaga ttataaattg ttaccaaaga aaactgtcta
     6001 tgaaaatatt gcttacgcta tggaagtaat cggggaaaat cgccgtaata tcaaaagacg
     6061 agtgatggaa gttttggact tggttggatt gaagcataag gttcgttctt tcccaaatga
     6121 actctcaggt ggggagcagc agcgaattgc gattgcgcgt gcaattgtaa ataatcccaa
     6181 agtattgata gctgatgagc caacaggaaa tctggatccg gataattcat gggaaattat
     6241 gaatctcttg gaacggatta acctacaagg gacaactatt ttgatggcga ctcataatag
     6301 ccagattgta aataccttgc gccaccgtgt cattgccatt gaaaatggcc gtgtcgttcg
     6361 tgacgaatca aaaggagagt atggatacga tgattagtag attttttcgc catttatttg
     6421 aagccttaaa aagtttgaaa cgaaatggtt ggatgacagt agctgctgtc agttcagtca
     6481 tgattacttt gaccttggtg gcaatatttg catctgttat tttcaataca gcgaaactag
     6541 ctacagatat tgaaaataat gtccgtgtag tagtttatat ccgaaaggat gtggaagata
     6601 atagtcagac aattgaaaaa gaaggtcaaa ctgttacaaa taatgactac cacaaggtat
     6661 atgattcttt gaagaacatg tctacggtta aaagtgttac cttttcaagt aaagaagaac
     6721 aatatgaaaa attaaccgag ataatgggag ataactggaa aatctttgaa ggagatgcca
     6781 atcctctcta tgatgcctat attgtagagg caaacactcc aaatgatgta aaaactatag
     6841 ccgaagatgc taaaaaaatt gaaggtgtct ctgaggttca agatggcggt gccaatacag
     6901 aaagactctt caagttagct tcatttatcc gtgtttgggg actagggatt gctgctttgt
     6961 taatttttat cgcagttttc ttgatttcaa ataccattcg tattaccatt atttcccgca
     7021 gtcgcgaaat tcaaatcatg cgcttggttg gagctaaaaa cagttatatc cgtggaccgt
     7081 tcttgttaga aggagccttt atcggtttat tgggagctat cgcaccatct gttttggtct
     7141 ttattgttta tcaaattgtt taccaatctg tcaacaaatc gttggtaggg caaaatctat
     7201 ccatgattag tccagattta tttagtccgt tgatgattgc cctactattt gtgattgggg
     7261 ttttcattgg ttcattggga tcaggaatat ccatgcgccg attcttgaag atttaggtaa
     7321 aatagctgct tttatgagga gattgtaaaa tctccttttt tgctacaaga gtttttgaaa
     7381 agagatgcgt agaagaaaag agcttccaaa gaagtccccc agagaagact tcttgatggc
     7441 taatttgaac ttgaaatttg gcgcactaga aatt
//
LOCUS       DAVBBS010000078         7220 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVBBS010000078 DAVBBS010000000
VERSION     DAVBBS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7220
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.078"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            231..645
                     /locus_tag="VQU32_001945"
                     /pseudo
     CDS             231..645
                     /locus_tag="VQU32_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(684..1127)
                     /locus_tag="VQU32_001946"
     CDS             complement(684..1127)
                     /locus_tag="VQU32_001946"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HET2473342.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(1157..3379)
                     /locus_tag="VQU32_001947"
     CDS             complement(1157..3379)
                     /locus_tag="VQU32_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET2473343.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            3790..4032
                     /locus_tag="VQU32_001948"
     CDS             3790..4032
                     /locus_tag="VQU32_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473344.1"
                     /translation="MTRFEEIFEEYKNPQDTFFYPLVYKENTYEKTAISIFALLMLGV
                     CCLFLFSQQSYKKLVQYYANDQNLPSRITYSEYSDK"
     gene            4372..5001
                     /locus_tag="VQU32_001949"
     CDS             4372..5001
                     /locus_tag="VQU32_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET2473345.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(5124..7016)
                     /gene="pepO"
                     /locus_tag="VQU32_001950"
     CDS             complement(5124..7016)
                     /gene="pepO"
                     /locus_tag="VQU32_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HET2473346.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         1985 a         1681 c         1378 g         2176 t
ORIGIN      
        1 ttatcatttt atgacaaaca tactatcgtt aaatgctgat ttaatcgaaa ttttgagtta
       61 taaactcctc agtttcgaac tcaaaattag ccgtgcagga taggataccc ttcataaact
      121 tatataacta ctttaatacc ttaaaaagct aataaaataa ggaatatcta agcaatatac
      181 attctaaaat acctgtacgg gagtgtctct aaagaaaagg aggttttagc atgaaaaaaa
      241 tcatcttcat caaaaccatt caactccttg tcattgatgg aatcatgctg gcatttttga
      301 catttaaaag gggggcttac ttgggactgg attttgattt atagtggttg gctcattttc
      361 tttcatcctg tgctattgac ctatctttca aaccaacttt gtgaccactt tagtcaactc
      421 tattcccaga ttagaccgag attctggcgt tttgctttac aaattctcct atgggatagc
      481 ctgatgattc tctccttggt gtctttaagt gatattccac ttttccttca gggaactctc
      541 ctcatcctag gacatctcat cccttcctat cgcatctgcc aaagcctgaa aagagacttc
      601 ccccaagcat atcaagaacc gatttctttt tggagtattt tatgatcgat gagaaagacc
      661 aagccgactg ggcttggtct ttcttatctc tttttagtat ctaggataat ggtaacaggt
      721 ccgttattaa ccagctcaac ctgcatatct gctccaaaga tacctgtctg aacgggcact
      781 tcttgcgcta atttttgatt gaaagcatca tagaagtctg atgccatatc aggtttagct
      841 gcccctgtaa aggctggacg attgcctttc ttagtatccg caaagagggt aaactgagaa
      901 atagagagga tttctccttc aatatctttg acagacaggt tcatcttgcc ttctgcgtct
      961 gaaaaaatcc gcatattgac cagttttctc acagcatagt ccaaatcttc ctcttggtcc
     1021 tctggtccaa caccaaccag caataaaagt ccctgattga tttttccctg aatctggcct
     1081 tctatactca cttgggcttt tttaacccgt tggataatga ttttcataat agcctttcta
     1141 gtaagagcta ggacaactag ccgttggtcc gtttgacaga gtaaacttct ggcacactct
     1201 taattttatc gacaaccgtg gtcagtgtag agaggttggc aataccgaag gacacatgga
     1261 tattagcaaa cttcatatcc ttggttggtt gggcattgac cgttgaaata ttcttggttg
     1321 tatttgaaag aacttgcagt acatcgttca acagtcctgt acggttgaga ccgtagatat
     1381 cgatatgggc catatactcc ttatttgagc tagagtactg gtcttcccat tccacatcaa
     1441 ggagacgttg ctcgtagttt tcttgggcac gcaggttcat acagtccaca cggtgaatag
     1501 ccacaccacg acccttggta atgtagccaa caatatcgtc accaggcacg gggttacaac
     1561 acttagcaat ccgcactagg agaccagaag caccttcaat aaccactccc ccctcatgct
     1621 tgaccttgag agtttcttta ttttcaacct tgacctcgcc acctttgaca agctcctctg
     1681 cctcagcctt ggccttggca cgctcttcct cacggcgttc cttttcagtc agacggttaa
     1741 agacggtaat cgcaccgatt tccccaaaac caatggccgc aaagagggag tcttctgtct
     1801 tgtaactggt cttttgcaga acttgatcca tgtggcgctt gtccataaat ttatttgcca
     1861 catagccatt ttcttggaac tgagccatca gcatctcacg acccttgttg acagacaatt
     1921 ccttatcttg gtttttaaag aactggcgaa tcttattgcg cgccttgcta gtcttgacca
     1981 tattgagcca gtcacggcta ggtccaaagg agttcgggtt ggcgataatt tcaacctgat
     2041 cccctgtctt taacttggtt gtcagtggaa ccatgcggcc attgaccttg gcaccagttg
     2101 ctttttcacc gaccttggta tggatttcgt aggcaaaatc aatcggtcct gaatctttgg
     2161 gaagagaacg gacagctcca tctggggtaa aaacgtaaat ctcctcagcc agatagtttt
     2221 ccttaacaga gtccacaaat tccttagcat catcagcctg gtcttggagc tccatcatct
     2281 ccttgatcca gttcattcca atagctgatt ccttgctgtt aacttgcccc tttatacctt
     2341 tcttataagc ccagtgagcc gcaaccccgt actcagccac ctcgtgcatt tccttggttc
     2401 gaatctggaa ttcaatcggc ccttttggtc cataaacagt cgtatggata gactgataac
     2461 cattagcctt gcggttggca atatagtctt tgaagcgacc tggcatcggt ttccaaaatt
     2521 catgcacgta accaagcatg gcataaacat cactttgggt atctaaaata caacgaatag
     2581 caatcagatc atagatttcc tcaaaccgtt ttctcttgtc ctgcattttg cggaaaattg
     2641 agtaaatatg cttgggacga ccataaatct tccctttcaa gtgacgttct gtcgtatact
     2701 cctctaattt tgtgactacc tcatccacca aggcctcacg ctccctgcgc ttttccttca
     2761 tcatatgggt aatcttgtaa aactccgttg gattgagata acggaaagac aagtcttcta
     2821 attcccattt gacactggaa atccccaaac gatgggcaag cggggcatag atttccatgg
     2881 tttctttgga aatacgctcc tgcttgtctt ttcgaagatg tttcagggtc cgcatattgt
     2941 gcaagcggtc agacagtttg accaaaataa cgcggatgtc ctcagacatg gccatgagca
     3001 tcttgcgatg attttccgct aattgctcct cgatcgattt gtactcgacc ttgccaagct
     3061 tggtaactcc gtcaacaatc atccgcacat caggaccaaa ctctctttcc aaatcgtcca
     3121 aagtcgcatc tgtatcttcc accacatcat gcaagaatcc acaagctact gttacagcat
     3181 ccagctttag cttagctaaa atacctgcca cttggatagg gtgaatgata taaggctcgc
     3241 ctgatttgcg atattgacca ctgtggcatt caacagcata gaccaaggcc ttatggacaa
     3301 aatgaacatc ctcttccgtt aaatattctt tggttaaagc gacaacttct tcgcctgtta
     3361 aattcacttc tttcggcatc tctactctcc aattcttcct accattttat cactttttta
     3421 agaatatgaa aactagattg gaacagaata agaaaaaaat aattcaaaat tgcttgataa
     3481 ttctgaatta ttggcccata atatactacg aagttagatt ttaaacttag gtgatagaag
     3541 gagagataga agaacggaaa ccatattgta acccaaagac tttctgattt ccccaattcc
     3601 attgaagata cgaaagataa acggtggaac tcgtatcaca tacactggta ccttgactgg
     3661 attttggaat taatactcaa tgaaaatcaa agagcaaact aggaaactag ccacaggttg
     3721 ctcaaagcac tgctttgagg ttgtagataa gactgacaaa gtcagtcaca tatatacggc
     3781 aaggcgacgt tgacgcggtt tgaagagatt tttgaagagt ataaaaatcc tcaagatact
     3841 ttcttctatc ctttagttta taaggagaat acctatgaaa aaactgctat ttctatcttt
     3901 gctctcctaa tgttaggagt ttgctgcctg ttcctattca gccagcaaag ctataaaaaa
     3961 ctcgttcaat actatgctaa cgaccagaac ctgcccagta ggataactta tagtgaatat
     4021 agcgacaaat gagaagccaa ctacggtagc actctaaaca tcacgtctat caaacaagct
     4081 aatgacggag tttatgcaac ctatgaaggg caattgacac ctttccaata ttgataaatt
     4141 gataaccagc ctgtcttcat ctagtcatgc tggtttttaa gttcatttta aatccttacc
     4201 tattctccct aactgtgcta tacttaattt atactcaatg aaaatcaaag agcaaactag
     4261 aaagctagcc gcaggctgtt caaagcactg ctttgaggtt gcagataaag ttgacgcggt
     4321 ttgaagagat tttcgaagag tattagtaca ttctttgaga ttggagttag tatgaaaatc
     4381 cataaaaccg tgaatcctgt tgcctatgaa aatacctatt atctagaagg cgaaaagcac
     4441 ctcatcgtcg tcgatcctgg tagtcattgg gaagccattc gtcagacaat cgagaagatc
     4501 aacaaaccga tctgtgctat tctcttgacc cacgcccatt atgaccatat catgagtctg
     4561 gacttggttc gcgagacgtt tggcaatcct cctgtctata tcgcagagag cgaagccagc
     4621 tggctctaca ctcctgtcga taatctctcc ggtctccctc gccacgatga tatggcagat
     4681 gtggtcacaa aacctgcaga acacaccttt gtctttcacg aagaatacca actagaggaa
     4741 tttcgtttta aggttctacc gaccccaggg cactctatcg gtggtgtttc cctagtcttt
     4801 cctgatgctc acctagtctt gacgggagat gctctattcc gcgaaactat cggacggacc
     4861 gaccttccga ctggtagcat ggagcaactc cttcatagta tccagaccca actcttcacc
     4921 ctaccaaact acgatgtcta tccaggacat ggtccagcta ctactatcgc tcacgaaaag
     4981 gccttcaatc cctttttcta gcaagatgat gacaatcgaa atttaagtaa actatccagc
     5041 aaatctttct attacaaaag gcatcctatc aaggttttca cacgtgattg gatgcctttt
     5101 ttctgatgac tagatttttt gtattaccaa ataatcacgc gctcctctgg tgaacgccac
     5161 attccgtctc cttctttgac atcataggtt gtaaagaaat cgtcgaagtt tggtacttgc
     5221 acattgacac ggagtttggc tggtgcgtgc acatcgacgc tagccaaaag tttcataaat
     5281 tctggtcgac ctttcatgcg ccagatgcga ccgaagttgt agaagaactc ttctgctgag
     5341 aagtctgctt ctctcttagc tgcttcaagc gctgctgcga ttcctcccaa gtcagccacg
     5401 ttttctgata cagtcaattt accgttaatg gttgctccat aagaatcctg tccatcaaat
     5461 tggtcaatga ctttttgtgt tttctccttg aaggcagcat agtcgctctc tgtccaccaa
     5521 tccttgaggc taccattttc gtcaaaggaa gccccgttag tatcaaaggc gtgggaaatt
     5581 tcatgggcaa tcactgcccc aataccaccg tagttagcag aagatgactg atgcaagtca
     5641 tagaaaggcg cctgtaaaat ggccgctgga aagacaatca ggttcttctg aggattgtag
     5701 taggcattga ccatatgagc aggcatgccc cattccttat agtctacagg ctggttccac
     5761 ttactccaac tgtgcttgat ttccacacgc gcaaaggcta gagcattctc aaaaagactg
     5821 gcagtttcat tcactacctt atccttgtaa cgtgcaggca attcttctgg atagccaata
     5881 taaggtttga tcacattgag cttcacgata gcctgtttac aggtttctgg agtgagccag
     5941 tcattcttaa gcagacgctc cttatagaca tcaatcatgg ttgccacttt tttctccaca
     6001 tccgccttgg cttctggaga gaacttctca cgggcgtacc aaagccccag ggcttgcttg
     6061 aaaggttcct gtgctagatg ataagctgct ttgaccttat cttttgcctc tggaactcca
     6121 gaaagggcac ggctgtaggc accagacaaa acacggatat cctctgttaa atagctggtt
     6181 gaaagattga caacactcaa aatcaaggtt gctttaagga gagaccaggc ttcctcactg
     6241 taaaattgct ctgctgcttg ccagaaacgt tcctcgtcta caataacctt gtctggtaat
     6301 tgcccaataa ctgctttgaa gaagtcatcc aaaggtaggg caggcgcgaa tttcttgaaa
     6361 tcttcgtaag aatatggatg atagagttta gcatattctg aactttcttc attagagagc
     6421 accactgccg caactcggcg gtccaattca agtctttttt ctagcaagtc ttcaatttct
     6481 tcatcagaga aatcataagc cttgaggaga tttgcgctgc tttctttcca aagagtcaag
     6541 agctcttcgc gctgaggatg ttcttctgca tagtaggtcg tatctggcaa gattgtgctt
     6601 ggagcgctag cccatagaac attgattcta gcatccataa agtctggcga tacaccaaaa
     6661 ggaaggaagt ttggttttcc tgcaagctca aactctgcta gtttagctgt aaaatccgca
     6721 aaagtttcca attcttggaa ttctttaagg agtggtaaga caggtgtgat accgtcagct
     6781 tctctcttgt caaaatcacg aactaggcgg tggtatttga caaagttttc caagatagca
     6841 tcctcaggca cttcttcacc tgctaaccac ttgtctgttg tcgccagcat caggtcttca
     6901 atttcctggt ctaaatcaac aaaacctcct gtttgagact tatctgctgg gatttcagct
     6961 gtctgttgcc attctccatt gatagcatca taaaaatcat cttgataacg tgtcatcttg
     7021 ttctcgcttt catttgtatt tgcatttatc ttaacaaaaa tcactaggga atgcaattaa
     7081 taaccattta actattattt tttagctatt ataaaaaacc tttccatgat aaaacgcata
     7141 attccaagtt ttttacactt gatactatgc gttttataat ttagaaattt atcctgaaag
     7201 ttatctttag aatctatttt
//
LOCUS       DAVBBS010000079         7162 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVBBS010000079 DAVBBS010000000
VERSION     DAVBBS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7162
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.079"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(741..1001)
                     /locus_tag="VQU32_001951"
     CDS             complement(741..1001)
                     /locus_tag="VQU32_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473347.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADIHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
     gene            complement(1060..1611)
                     /locus_tag="VQU32_001952"
     CDS             complement(1060..1611)
                     /locus_tag="VQU32_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473348.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIYEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(1601..1798)
                     /locus_tag="VQU32_001953"
     CDS             complement(1601..1798)
                     /locus_tag="VQU32_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473349.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(1802..2386)
                     /locus_tag="VQU32_001954"
     CDS             complement(1802..2386)
                     /locus_tag="VQU32_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473350.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEIPKELTPSEQLTWIAFLLPDGDIKQIKSVSNLLYGGAYALS
                     HSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLK"
     gene            complement(2794..3219)
                     /locus_tag="VQU32_001955"
     CDS             complement(2794..3219)
                     /locus_tag="VQU32_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473351.1"
                     /translation="MTSRVFIDADCISAFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNYDSNKIIGKGEATSIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(3212..3706)
                     /locus_tag="VQU32_001956"
     CDS             complement(3212..3706)
                     /locus_tag="VQU32_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473352.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMVLGHYIKSTEQLLENNRISQGKYEELLLDAFRYDIVYGLDEEGE
                     LSFD"
     gene            complement(3752..3958)
                     /locus_tag="VQU32_001957"
     CDS             complement(3752..3958)
                     /locus_tag="VQU32_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473353.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            complement(3945..4106)
                     /locus_tag="VQU32_001958"
     CDS             complement(3945..4106)
                     /locus_tag="VQU32_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2473354.1"
                     /translation="MNMINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(4471..5427)
                     /gene="lytA"
                     /locus_tag="VQU32_001959"
     CDS             complement(4471..5427)
                     /gene="lytA"
                     /locus_tag="VQU32_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HET2473355.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPDFTVEPEGLITVK"
     gene            complement(5803..7155)
                     /locus_tag="VQU32_001960"
     CDS             complement(5803..7155)
                     /locus_tag="VQU32_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET2473356.1"
                     /translation="MDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVGRFLGQE
                     SLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWILGALLSI
                     LVMLLGFLGLYHLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLRSIGDSL
                     AALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRKSVPELL
                     PQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQTAARRI
                     MTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIFLFFASP
                     ALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPLVSSFIE
                     LIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAILATKVQS
                     "
BASE COUNT         2298 a         1486 c         1132 g         2246 t
ORIGIN      
        1 tggtttcgca ggaagtctat tatatatgca cagacatatg aaatcttttt gagacagcag
       61 ttatgatata tttatccatt cacaacatct tctgtactaa tactacaata ttggtacatt
      121 ttgattacac aatcgtttca aaaaatatga tggaagcctg agtattttat agtaattccc
      181 ttcctatcaa ctagatcaac cagttgagtg ttgaatgttg aaacacccta gcctcagcaa
      241 aggactatga tcagcatttc ctagccaata ggacgctcca aatcacctat tctaagtcca
      301 tcactagctt ggtcagtaag atacagactg caaggcgaga ctgacgtggt tgatgagatt
      361 ttggaagagt attacaatca tagattcacc agtagacttg accgcagatt taactatagt
      421 agattgaaac tagaatagta catctctgct tctaaaacat tgttagaaat cgatttgact
      481 gtcctgatca atttgtcctg ttctcgtttc atttcactat actaaaaaac cacctgaaac
      541 aaatcaagtg actagaggat aaaaacaaag actaggcaaa aagttttgaa aagcaaaaaa
      601 cgcataatat caggtcttga aaaaccttga tactatacgt ttgattgtgg gaagattgac
      661 ttcattttct cctgaaattg aagttttttc tagtttccag atatagacga attcaatata
      721 gccatctcgc tacaattatt ttaaccatcg agaaaatttt tcagaatttc aaaaatttca
      781 ttttgatcca atgaaatgtc atcattttga agaatagttg acttcacagg ctctaattct
      841 atcacaaagc ggtgtatgtc tgcttttacc gcactacttg atgttatagc cgataacttt
      901 tcatctccta gcaaggaagc tgtcaaacgg gtaaggtcat tcaaatgctt tttaatggac
      961 ttacttaaac cttgagcacc tgtagcagag cgttccctca tatccagcca agctttagcc
     1021 ttaaaaataa ttaatactca atgaaaatca aagagcaaac taggaagcta gctgcaggct
     1081 gctcaaaaca ctgttttgag gttgtagatg gaagctgacg tggtttgaag agattttcga
     1141 agagtataaa ccacaattac tcaataccga atacccctga atggtttctt tttcatgcac
     1201 caatatatta taataatctt catccaataa taaggctgat aaactagcat cttggtcaaa
     1261 atgtaaggga atttctcgac cgtccttctt taatggatat tctggtaaga tactaaatag
     1321 ttcaatcata gaaggaaact caggattagt tgttgtaaat cgaaaaagct gcgctttctc
     1381 atctttctga cttccttgat actctcccaa ttctaaaaaa tgattcaagg tagtataaaa
     1441 ttccttattt tttacttcat aaatgatgac catatcataa tcttttgttg tgcgactttt
     1501 aaatccttgc gaatccaata cgatagaggt agcagttccc ccaatcagaa cataatagtt
     1561 ctgaaaatcc gcaaacattt cttgaaaaat aactttcgta ttagctggca tcatcttctc
     1621 ccagatactg taatatcata ttttctagtg cttcactctc ttcctctata cgtgggtcat
     1681 catcatcttt taaggtcaaa taaagagaaa tcggatctac aaattgttta tcatgattat
     1741 ttttaaaatc attccaaaac tcagatacaa aaggacgata tttccatatc tctagcatct
     1801 ttcattttaa aacatgctga gaaagtggca aggataactg attgaatttt ctctgccata
     1861 tgacatagct agtattttca tccgtttcag ctaaaaaagt tgaatgcgac aaagcataag
     1921 caccaccata tagaaggtta gaaacagatt ttatctgctt tatatcgcca tctggcaata
     1981 aaaaggcaat ccacgttaat tgttcgctag gtgttaattc cttagggatt tcagtatcat
     2041 tcgcattgag tactagtccc aatggaggga agaagaggtt tcccttaaag tctacaaacg
     2101 gaactctagc ttgaagtaat tgcttttttt cactgtctga taacttcgaa aacaccaaaa
     2161 caacatccat attggctttt tcacccatag tgcgagcctg agtaacaaat gaactcaaac
     2221 tcccccttct cttttcttta atcaataaaa atgattcctt atgaaaagtc atttgagtat
     2281 actcaaacct ttttacaaaa ctgattggta aagtcaaagt cagttcctca atactgacat
     2341 caacataaaa ggattgaaaa attgacatta tctttttgac attcatgttt tttccttatt
     2401 ctatcatttt tattatattt tatcattcgg tataataaaa tataagtttt ttgataaaat
     2461 aaccaaaaac tactcgctat cccctctcac attaccctac atatcgtttg acatgcgact
     2521 gatagttcag gaaaacttca aggagaactt ttctctcatc cactatgcag gacttactat
     2581 ttcacttcta ctcctatagg ctcaatttga cacttttctt tttcgaattg ctcatatgcc
     2641 tcctgaatag tgagttcgat ttatccaaat ttatttttgt ctattttgat gaatacttcc
     2701 acgaagatag tctgaaaatg aatttgcacc aattttcctt ctctttttaa gcatattatt
     2761 ccagatatga ttgccctctt gttcagtaat aaattacgct ttaaacgctt caatcagtat
     2821 atctcctgtt gtcatatgtt ctaaagaaaa ttcttctaca tatggtttaa catctcttag
     2881 gttattactt cctaatatcc cattatgctt tttcgctaag aaaatagaag tcgcttctcc
     2941 cttaccaata atcttgttac tatcataatt tcttgttaaa tctctatata atgcgtattc
     3001 ttcagttcca atgtctatgc tcacaatctc agctgaaccc ttagttacca actggtctat
     3061 cctagatttt aaatggggaa ttgtaggtat attgatttca tcatacacct cttgtggaat
     3121 aacaatttta cccgaataga gcttttctaa aagatgttca gtgccaaccc ataaaaatgc
     3181 tgaaatacaa tctgcatcaa taaatacacg actagtcaaa cgacaactcc ccctcttcat
     3241 ctagcccata tacaatatca tatctgaaag catctagtaa cagttcctca tacttccctt
     3301 gcgaaattct gttattttct aaaagttgtt cagtcgattt aatatagtgc cctaatacca
     3361 tttccttttt actttctgac aaaggacgat ataaacttgt atcatagcct aatcttgaag
     3421 ctgtctctat aacactaata tccatatttt taatttcttc tgcatcaagg tatccatcat
     3481 ttctcaatct atataacata gctttatgac tgataccata aaactgaccc aattttataa
     3541 tatcttctac ttcaagatga gttctattgg cattttctct gatttcctca accatcctat
     3601 acagtgaaga tgggaaaatt aaaaaataag aagcaaactg atccgctttt ctttcagttt
     3661 catctccttc accaatcaag ataagactga ctgaactctt cttcacctca tcataataaa
     3721 gatgatacag tccatgtgct aaagaaaatc tttaccttcc taatggcatg tctgaattga
     3781 ctgcaatgag actgaactga gttcctttat aacagactcc gctaatattc tttccgagtc
     3841 cataaaatac cagcgtcaaa ttttctatct tttgtaccaa tttaaaaata tctatcggcg
     3901 attcaccatc agctcccaac ttttttctaa gatttgaagc tttattactt aaatccattc
     3961 gtgatattcc tttcaccttt ttccattttg gcaatcatgc tttgattcaa agacaaatac
     4021 tcagcaatct tatcttgagt aagatttgaa gatattctaa gttcttttat tctttttcca
     4081 acatcacata catttatcat attcatacac ctctcataaa aagaatagca cattcttgtc
     4141 ataatttttt aaataaaaaa attatgacaa aacaaggaag caatttattg atgctactta
     4201 aaaatctaaa attgatgata ttaaacctat ttgatgagtt cctatcaaaa atcgtatctt
     4261 caacctcaaa acagtactta aagctatccg actcggttta cattgtcaaa tttagatttt
     4321 atttgagcat aactttctag tttgcttttt gatttttgtt taatatagta acaaaaattg
     4381 aaggaaatat ctccacaaga aaacgcatac tattaagctt tttcaagacc taataatatg
     4441 cgctgttctg atttgaaaga cattccatta ttattttact gtaatcaagc cttcaggctc
     4501 tactgtgaag tctggcttgt ctgccagtgt tccgtctggt ttgaggtagt accagcctgt
     4561 tccgtccgct gactggataa aggcatttga taccatggcg ccttctttag cgtctaagta
     4621 gtaccaagtg tccttgtact tgacccagcc tgtcttcatg gcaccttctt cgttgaaata
     4681 gtaccactta tcagcgattt tcttccagcc tgtagccatt tcgcctgagt tgtcgaacca
     4741 gtaccagttg ccgtctgtgt gcttcctcca gcggtctgca agcatatagc ctgaactgtc
     4801 aaagtagtac caagtgccat tgattttctc aaacttgtct tttggataag agccgtctga
     4861 atgtacgtac cagtagccag tgtcattctt ctgccagcct gtttcaatcg tcaagccgtt
     4921 ctcaatatca tgcttaaact gctcacggct aatgccccat ttagcaagat atggataagg
     4981 gtcaacgtgg tctgagtggt tgtttggttg gttattcgtg caatactcgt gcgttttaat
     5041 tccagctaaa ctccctgtat caagcgtttt cggcaaacct gcttcatctg ctagattgcg
     5101 taagagttcg atataaaggc ggtagtccgt catgaactct tctttggttg aatggctttc
     5161 aatcagttca accgctgcat aggtctcagc attccaaccg cccccaacgt cccaggcacc
     5221 attatcaaca ggtcctacct gcatgatgca accgttccca acaatgtgcg agaaaaaacc
     5281 taattctggg tctttccgcc agtgataatc cgcttcattc tgtacggttg aatgcggatt
     5341 cccagttgag tgtgcgtgta cttgcctata tggttgcacg ccgacttgag gcaaatctgt
     5401 tcttaattta ctcacattaa tttccatatt ctactcctta tcaattaaaa caactcattt
     5461 tttacaatcc agaaccagaa actcctttat ttctacctta caaagaagac aatcttagtc
     5521 acgattaagc ttgtagatag aacctcaaaa cgcactattt tgacactgta aataggactg
     5581 acaaggtctg cattctatct acaataacac cccagactaa aaagcttttc aaaagtatat
     5641 ttttacagtc tctatatgtc cttttcataa atactatact ttattatatc acataaaaga
     5701 agtcaaaagt ctgttaaact attttcaaca ccaaactaaa gaagagaaca caagtttttt
     5761 cgatgttcac tagaggaaat ggattttatt cagtaaatcc aactaggatt gcactttggt
     5821 tgccaaaatt gcctttcctt cttttatcaa gggatgacgg aatagtgaga agtacagttg
     5881 aactgtcatg gcaacccaga taagaggttc acaaaggata acacccttat atcctgccca
     5941 aggaataatc aaaaccacaa aaacgatttt tccgattagt tcaataaagc tagaaactag
     6001 aggaaggatc ttctgcccca agccctgcaa gcaattgcga taaatcaaca agaggctcaa
     6061 aatgggataa aaggctgaac tgatttgcag atagagactt ccattttcta tcaagtaacc
     6121 atctgtcgaa ctagccaaga aggaaaccaa ggctggactg gcaaaaaaga ggaaaataca
     6181 aacaaaaact gcccaggata tacttaaacg actgccgatt cgaagacctt gaacaatgcg
     6241 gtcaggtcgc ttagctccta gattctgaga agcaaaggtc gtcattgatg cagaaatagc
     6301 ggtcatagga agaagggcaa aggtcataat gcgtcgagct gccgtctggg cactaataat
     6361 cactgcacca aatgtattaa cagaaaactg taaaatcaca ctgccgatag atacaattga
     6421 actcatcaag cccatagcca aaccttgctc caagagatcc gcgtacaagc ttttgtccca
     6481 tttgaaatgt ttaaactgtg gcaagagttc tggcacactt ttacgaatat aataaaagca
     6541 gagaaccgct gataaacctt gcgaaataat ggtagcaagt cctgcggatt gaactcccag
     6601 atgcaattgc gtaataaaat agagatccag aaccacatta accaaggcag agaaaatcag
     6661 aaatcccagg gctgctagac tgtcaccaat agaccgcaac aagcctgcaa aaagattata
     6721 agcaaagctg acacctacac aggtcacaat catagaaata tattgataag attgaggaag
     6781 aatttctgca ggagtatcta agtattgcaa gagatgatac aagccaagaa agcccagcaa
     6841 cataactaga atgctcaaaa gagcacctaa aatccaagta gctgctactg cttccttgat
     6901 tttagtgaaa ttccgagccc cataataacg agcaatgaca atccccatgc cattgccaac
     6961 accaagtgta aaacctacaa tcaggtcaaa aatcgctgtc gtcgctccta ctgcagccaa
     7021 ggattcttga ccaagaaatc gtccaacaat caagacatca gcagtgttat agagctgttg
     7081 aaaaatattt gatagcaaaa ttggaaaggt gaagcttaag agcgagggaa gaatcggacc
     7141 atgtatcagg tccactgttc gt
//
LOCUS       DAVBBS010000080         7014 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVBBS010000080 DAVBBS010000000
VERSION     DAVBBS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7014
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.080"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(317..2008)
                     /locus_tag="VQU32_001961"
     CDS             complement(317..2008)
                     /locus_tag="VQU32_001961"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET2473357.1"
                     /translation="MNTKELIASELASIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            2144..2590
                     /gene="argR"
                     /locus_tag="VQU32_001962"
     CDS             2144..2590
                     /gene="argR"
                     /locus_tag="VQU32_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2473358.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            2641..5175
                     /gene="mutS"
                     /locus_tag="VQU32_001963"
     CDS             2641..5175
                     /gene="mutS"
                     /locus_tag="VQU32_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET2473359.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKLDSQNNFLVAIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSCQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPALAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLITGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTENPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            5322..>7014
                     /locus_tag="VQU32_001964"
     CDS             5322..>7014
                     /locus_tag="VQU32_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2473360.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDVPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
BASE COUNT         1901 a         1370 c         1667 g         2076 t
ORIGIN      
        1 tttggttagg tgaattagtg aagcgtttag gcaagtgtct ctggttacga cgtcatggac
       61 tctaaatcga ttatatttag gggtcatgac tagtgaagca gttagctagt tcgcatataa
      121 gcggctagcg tctaacaatt aggaacttta gttccaataa ctttaagatt acgacgtttt
      181 aggacataaa tcgatcatat ttatgtccta aaactagtga agtatgtcct aaaactagtg
      241 aagcgcctag ccaaagtccg aataggattt ggcgttagtt acttagattg ctttgcaatc
      301 aagtaacttt ggcgatttac atcttctctg gcgcttctac tccaagcaag cgaagggctt
      361 ctttgagaac gactgcggtt gcgtagctga gggctagacg gctgtcgcgt tctgggcttt
      421 catccaagat acgtgtatgt gcatagtatt tgttaaagga ttgagccagg ctaattgcaa
      481 atttagcaat gatagaaggt tcaaagttat ctgccgcacg gttgataata cgtgggaagt
      541 cttgaatgag tttaatgatt tcccagcttt cagtatcatt caagctatag ttgccagctg
      601 tttctggttt gaaatcggct ttgcgtaaga tagattggat acgagcgtag gcatattgaa
      661 cgtaaggtcc agtttcaccc tcgaaggata ccatagcctc taggtcgaag tcgtatccat
      721 ttgtacggtc ggttttgagg tcatagaatt taatggctcc aaccccaaca gcatgtgcta
      781 cttggtcttt gttttctagt tcaggatttt tagcctcgat ttggaccttg gcacggctaa
      841 cagcctctgc aacagtaggc tctagcaaga tgacattccc tttacgagta gagagtttct
      901 tcccttcttt tgtaaccaaa ccaaaaggaa cgtgagtaat gtcgtcactc cagtcgtagc
      961 ccatctcttg caagacagct ttgagctgtt taaagtgggc agattgttct tgaccaacga
     1021 catagataga tttagcaaat tggtattcgt ttttacggta gagggctgca gccaagtcac
     1081 gtgtgatata gagagttgca ccatcagact tcttgatgag ggctggatgt tcaattccgt
     1141 atttctcaag attcacaact tgggcacctt ctgattcaag aagtagtcct ttttcagaaa
     1201 gaatgtctac aactgcatcc atcttatcat tgtagaaggc ttctccgtta tagctgtcaa
     1261 attcaacctt caattcattg taaaggcggt taaattccac taaactttca tcgcggaacc
     1321 attgccaaag agcgagagct tcctcatctc cattttcaag tttacggaac cattcgcgcg
     1381 cttcttcatc caagctaggg tcattttcag cttcagcgtt gatgcggaca tagagtttaa
     1441 ggagttcatc gattggatga gcttttacag cttcttcgtc gccccatttt ttgtaggcaa
     1501 caatcaacat cccaaattgt ttaccccagt ctcccaaatg gttgaccttg accgtttgat
     1561 aaccgatttt ttggaaaata tgtgacaagc tatctccgat aacagttgaa cgcaggtggc
     1621 caatagaaaa tggtttagcg atattcggac tagacatgtc gataacaaca ttttcttgtt
     1681 taccaatatt ttggtcagca tagtgttctt tttcagtggt aacagcttgc aatacttgag
     1741 cagaaatggc agatttatca aggaaaaagt taacgtaagg tcctgttgcg acaacttttt
     1801 caaaggcttg gctgttcatt ttttcagcca gttcagccgc aatcatttgt ggtgctttac
     1861 gttcgacttt ggcaagagaa aaagcaggga aagcgatgtc acccatttct gagtttttag
     1921 gggtttccag taactttaaa atagcctctt ggtccaggct atcaatgatg ctagccaatt
     1981 cgctagcaat caattctttt gtattcatta agagctcctt tttggacttt tctactattt
     2041 tatcacaatt ttaaagaaag aagaaaaaat ttttgaaatc tcctgttttt ttggtataat
     2101 atggttataa atatagttat aaatatgcac gcaagaggat tttatgagaa aaagagatcg
     2161 tcatcagtta ataaaaaaaa tgattactga ggagaaatta agtacacaaa aagaaattca
     2221 agatcggttg gaggcgcaca atgtttgtgt gacgcagaca accttgtctc gtgatttgcg
     2281 cgaaatcggc ttgaccaagg tcaagaaaaa tgatatggtg tattatgtac tagtaaatga
     2341 gacagaaaag attgatttgg tggaattttt gtctcatcat ttagaaggtg ttgcaagagc
     2401 agagtttacc ttggtgcttc ataccaaatt gggagaagcc tctgttttgg caaatattgt
     2461 agatgtaaat aaggatgaat ggattttagg aacagttgct ggtgccaata ccttattggt
     2521 tatttgtcga gatcagcacg ttgccaaact catggaagat cgtttgctag atttgatgaa
     2581 agataagtaa ggtcttggga gttgctctca agacttattt ttgaaaagga gagacagaaa
     2641 atggcgatag aaaagctatc acccggcatg caacagtatg tggatattaa aaagcaatat
     2701 ccagatgctt ttttgctctt tcggatgggt gatttttatg aattatttta tgaggatgcg
     2761 gtcaatgctg cgcagattct ggaaatttcc ttaacgagtc gcaacaagaa tgccgacaat
     2821 ccgatcccta tggcgggtgt tccctatcat tctgcccaac agtatatcga tgtcttgatt
     2881 gagcagggtt ataaggtggc tatcgcagag cagatggaag atcctaaaca agcagttggg
     2941 gttgttaaac gagaggttgt tcaggtcatt acgccaggga cagtggtcga tagcagtaag
     3001 ctggacagtc agaataattt tttggttgcc atagaccgcg aaggcaatca atttggccta
     3061 gcttatatgg atttggtgac gggtgacttt tatgtgacag gtcttttgga tttcacgctg
     3121 gtttgtgggg aaatccgtaa cctcaaggct cgagaagtgg tgttgggtta tgacttgtct
     3181 gaggaagaag aacaaatcct cagctgccag atgaatctgg tactctctta tgaaaaagaa
     3241 agctttgaag accttcattt attggatttg cgattggcaa cggtggagca aacggcatct
     3301 agtaagctgc tccagtatgt tcatcggact cagatgaggg aattgaacca cctcaaacct
     3361 gttatccgct acgaaattaa ggatttcttg cagatggatt atgcgaccaa ggctagtctg
     3421 gatttggttg agaatgctcg ctcaggtaag aaacaaggca gtcttttctg gcttttggat
     3481 gaaaccaaaa cggctatggg gatgcgtctc ttgcgttctt ggattcatcg ccccttgatt
     3541 gataaggaac gaatcgtcca acgtcaagaa gtagtgcagg tctttctcga ccatttcttt
     3601 gagcgtagtg acttgacaga cagtctcaag ggtgtttatg acattgagcg cttggctagt
     3661 cgtgtttctt ttggcaaaac caatccaaag gatctcttgc agttggcgac taccttgtct
     3721 agtgtgccac ggattcgtgc gattttagaa gggatggagc aacctgctct agcctatctc
     3781 atcgcacaac tggatgcaat ccctgagttg gagagtttga ttagcgcagc gattgctcct
     3841 gaagctcctc atgtgattac agatggggga attatccgga ctggatttga tgagacttta
     3901 gacaagtatc gttgcgttct cagagaaggg actagctgga ttgctgagat tgaggctaag
     3961 gagcgagaaa actctggtat cagcacgctc aagattgact acaataaaaa ggatggctac
     4021 tattttcatg tgaccaattc gcaactggga aatgtgccag cccacttttt ccgcaaggcg
     4081 acgctgaaaa actcagaacg ctttggaacc gaagaattag cccgtatcga gggagatatg
     4141 cttgaggcgc gtgagaagtc agccaacctc gaatacgaaa tatttatgcg cattcgtgaa
     4201 gaggtcggca agtacatcca gcgtttacaa gctttagccc aaggaattgc gacggttgat
     4261 gtcttacaga gtctggcggt tgtggctgaa acccagcatt tgattcgacc tgagtttggt
     4321 gacgattcac aaattgatat ccggaaaggg cgccatgctg tcgttgaaaa ggttatgggg
     4381 gctcagacct atattccaaa tacgattcag atggcagaag ataccagtat tcaactgatt
     4441 acagggccaa acatgagtgg gaagtctacc tatatgcgtc agttagccat gacggcggtt
     4501 atggcccagc tgggttccta tgttcctgct gaaagcgccc atttaccgat ttttgatgcg
     4561 atttttaccc gtatcggagc agcagatgac ttggtttcgg gtcagtcaac ctttatggtg
     4621 gagatgatgg aggccaataa tgccatttcg catgcgacca agaactctct cattctcttt
     4681 gatgaattgg gacgtggaac tgcaacttat gacgggatgg ctcttgctca gtccatcatc
     4741 gaatatatcc atgagcacat cggagctaag accctctttg cgacccacta ccatgagttg
     4801 actagtctgg agtctagttt acaacacttg gtcaatgtcc acgtggcaac tttggagcag
     4861 gatgggcagg tcaccttcct tcacaagatt gaaccgggac cagctgataa atcctacggt
     4921 atccatgttg ccaagattgc tggcttgcca gcagaccttt tagcaagggc ggataagatt
     4981 ttgactcagc tagagaatca aggaacagag aatcctcctc ccatgagaca aactagtgct
     5041 gtcactgaac agatttcact ctttgatagg gcagaagagc atcctatcct agcagaatta
     5101 gctaaactgg atgtgtataa tatgacacct atgcaggtta tgaatgtctt agtagagtta
     5161 aaacagaaac tataaaacca agactcacta gttaatctag ctgtagcaag gagacttctt
     5221 tgacaattct ccactttttt gctagaatga catcacacaa acagaatgaa aaggagctga
     5281 cgcattgtcg ctcccttttg tctatttttt aaggagaaag tatgctgatt cagaaaataa
     5341 aaacctacaa gtggcaggcc ctggcttcgc tcctgatgac aggcttgatg gttgctagtt
     5401 cacttctgca accgcgttat ctgcaggaag tcttaggcgc cctccttact gggaaatatg
     5461 aagctattta tagtatcggg gcttggttga ttggtgtggc cgtagtcggt ctagttgctg
     5521 gtggactcaa tgttgtcctc gcagcctata ttgcccaagg agtttcatcc gaccttcggg
     5581 aggatgcctt ccgtaaaatt caaacctttt cttatgctga tattgaacaa tttaatgcgg
     5641 gaaatctagt cgttcgaatg acaaatgata tcaaccagat tcagaacgtt gtcatgatga
     5701 ccttccagat tcttttcaga cttcccctct tgttcatcgg ttcgtttatc ctagcggttc
     5761 aaaccttacc ttctctgtgg tgggtgattg ttctcatggt agtcttgatt tttggtttga
     5821 ctgctgtcat gatgggaatg atggggcctc gttttgccaa gtttcaaacc cttcttgagc
     5881 gcatcaatgc cattgccaag gaaaatttac gtggcgttcg tgtggtcaag tcctttgtcc
     5941 aagaaaaaga gcaatttgct aagtttacag aggtctcaga cgagcttctt ggtcaaaacc
     6001 tttacattgg ttatgccttt tcagtagtgg aaccctttat gatgttggtt ggttacgggg
     6061 cggtcttcct ctctatttgg ctggtcgcgg gaatggttca gtcggatccg tctgttgttg
     6121 gttccatcgc ttcttttgtt aattacctaa gccagattat ctttaccatt gttatggttg
     6181 gatttttggg aaattctgtc agccgtgcca tgatttccat gcgtcgtatt cgagaaattc
     6241 ttgacgcaga gccagctatg accttcaagg atgtcccaga tgaagagttg gttggaagcc
     6301 ttagctttga aaatgtgacc tttacctatc caatggacaa ggaaccgatg ctgaaagatg
     6361 tgagctttac tattgaacct ggtcaaatgg ttggtgtagt tggagcgact ggtgcaggaa
     6421 agtcaacctt ggctcaattg attccacgtc tctttgatcc acaggaaggg gctatcaaaa
     6481 tcggaggtaa ggatattcga gaagtgagtg aaggaaccct gcgtaaaaca gtttccatcg
     6541 ttctccaacg tgccattctt tttagtggaa cgattgcaga taacttgaga caggggaagg
     6601 gaaatgctac tctatttgaa atggagcgcg cagccaatat tgcccaggct agtgaattca
     6661 ttcatcgtat ggagaaaacc tttgaaagtc cagttgaaga acggggaacc aatttctctg
     6721 gtggacaaaa acaaaggatg tcgattgcgc gtgggattgt cagcaatcca cgtattctga
     6781 tttttgatga ttcgacctca gccttggatg ccaaatcaga gcgcttggtg caagaagctt
     6841 tgaataagga cttgaagggg acgacaacca ttattattgc tcaaaaaatt agctcggttg
     6901 tccatgcaga caagatcttg gttctaaatc aaggacgatt gattggtcaa ggcacgcatg
     6961 cagacttggt tgccaacaat gccgtttacc gtgaaatcta tgaaacacag aaat
//
LOCUS       DAVBBS010000081         6989 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVBBS010000081 DAVBBS010000000
VERSION     DAVBBS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6989
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.081"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            226..6657
                     /locus_tag="VQU32_001965"
     CDS             226..6657
                     /locus_tag="VQU32_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET2473361.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VVDKETENKEKHEDIHNAIETSQDFKEKKTAVIEGKEVVSKNPVTDNNTSNEEARIKE
                     DSNQSQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPYGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANHADEKG
                     MISYYLVDPDKDASKYEKLGEISEDKLKNAKSPEENTNNNQAKDEDSKPDEKSSVEGE
                     ASLEINKTISTIREFENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKGNIIAY
                     DDGSALQYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIEI
                     KATKYDSHSKTMIFDLYANINDIVDGLAFAGDMRLFVKDDNQIKAETKIRMPEKNKET
                     KAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGY
                     ALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQ
                     AVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKS
                     NVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWE
                     LRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDG
                     NVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRN
                     NGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTG
                     EVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKD
                     AGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQ
                     REDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVG
                     IFLGLKKKNQD"
BASE COUNT         2759 a          943 c         1348 g         1939 t
ORIGIN      
        1 taatcttatt ttttaacgtg tgctaaaaaa taaattaatg catgttaatt tttcttgaat
       61 taaatttttt taaatgatat actgttttta tggagagata acaattatat ataaatgtac
      121 tactctattt cctagataat acttagtaaa actttttata acaaaggcta gcaaagttaa
      181 agtttattat ctattggaag ttaaataaat atgtaaggaa ttaaaatgaa aaaaagtaca
      241 gtattgtcat taaccacagc tgcagttatt ttagcagcct atgcccctaa tgaggtagtc
      301 ttagcagaca catctagctc tgaagatgct ttaaacatct ctgataaaga aaaagtagta
      361 gtagataagg aaacagaaaa taaagagaaa catgaagata ttcataatgc tatagaaact
      421 tcacaggatt ttaaagagaa gaaaacagca gtcattgagg gaaaagaagt tgttagtaaa
      481 aatcctgtga cagacaataa cactagcaat gaagaagcaa gaatcaaaga agattccaat
      541 caatcccaag gagatcatac ggactcattt gtgaataaaa acacagaaaa tcccaaaaaa
      601 gaagataaag ttgtctatat tgctgaattt aaagataaag aatctggaga aaaagcaatc
      661 aagcaactat ccaatcttaa gaatacaaaa gttttatata cttatgatag aatttttaac
      721 ggtagtgcca tagaaacaac tccagataac ttggacaaaa ttaaacaaat agaaggtatt
      781 tcatcggttg aaagggcaca aaaagtccaa cccatgatga atcatgccag aaaggaaatt
      841 ggagttgagg aagctattga ttacctaaag tctatcaatg ctccgtttgg gaaaaatttt
      901 gatggtagag gtatggtcat ttcaaatatc gatactggaa cagattatag acataaggct
      961 atgagaatcg atgatgatgc caaagcctca atgagattta aaaaagaaga cttaaaagga
     1021 actgataaaa attattggtt gagtgataaa atccctcatg cgttcaatta ttataatggt
     1081 ggcaaaatca ctgtagaaaa atatgatgat ggaagggatt attttgaccc acatgggatg
     1141 catattgcag ggattcttgc tggaaatgat actgaacaag atatcaaaaa ctttaacggc
     1201 atagatggaa ttgcacctaa tgcacaaatt ttctcttaca aaatgtattc tgacgcagga
     1261 tctgggtttg cgggtgatga aacaatgttt catgctattg aagattctat caaacacaac
     1321 gttgatgttg tttcggtatc atctggtttt acaggaacag gtcttgtagg tgagaaatat
     1381 tggcaagcta ttcgggcatt aagaaaagca ggcattccaa tggttgtcgc tacgggtaac
     1441 tatgcgactt ctgcttcaag ttcttcatgg gatttagtag caaataatca tctgaaaatg
     1501 accgacactg gaaatgtaac acgaactgca gcacatgaag atgcgatagc ggtcgcttct
     1561 gctaaaaatc aaacagttga gtttgataaa gttaacatag gtggagaaag ttttaaatac
     1621 agaaatatag gggccttttt cgataagaat aaaatcacaa caaatgaaga tggaacaaaa
     1681 gctcctagta aattaaaatt tgtatatata ggcaaggggc aagaccaaga tttgataggt
     1741 ttggatctta ggggcaaaat tgcagtaatg gatagaattt atacaaagga tttaaaaaat
     1801 gcttttaaaa aagctatgga taagggtgca cgcgccatta tggttgtaaa tactgtaaat
     1861 tactacaata gagataattg gacagagctt ccagctatgg gatatgaagc ggatgaaggt
     1921 actaaaagtc aagtgttttc aatttcagga gatgatggtg taaagctatg gaacatgatt
     1981 aatcctaata aaaaaactga agtcaaaaga aataataaag aagattttaa agataaattg
     2041 gagcaatact atccaattga tatggaaagt tttaattcca acaaaccgaa tgtaggtgac
     2101 gaaaaagaga ttgactttaa gtttgcacct gacacagaca aagaactcta taaagaagat
     2161 atcatcgttc cagcaggatc tacatcttgg gggccaagaa tagatttact tttaaaaccc
     2221 gatgtttcag cacctggtaa aaatattaaa tccacgctta atgttattaa tggcaaatca
     2281 acttatggct atatgtcagg aactagtatg gcgactccaa tcgtggcagc ttctactgtt
     2341 ttgattagac cgaaattaaa ggaaatgctt gaaagacctg tattgaaaaa tcttaaggga
     2401 gatgacaaaa tagatcttac aagtcttaca aaaattgccc tacaaaatac tgcgcgacct
     2461 atgatggatg caacttcttg gaaagaaaaa agtcaatact ttgcatcacc tagacaacag
     2521 ggagcaggcc taattaatgt ggccaatgct ttgagaaatg aagttgtagc aactttcaaa
     2581 aacacggatt ctaaaggttt ggtaaactca tatggttcca tttctcttaa agaaataaaa
     2641 ggtgataaaa aatactttac aatcaagctt cacaatacat caaacagacc tttgactttt
     2701 aaagtttcag catcagcgat aactacagat tctctaactg acagattaaa acttgatgaa
     2761 acatataaag atgaaaaatc tccatatggt aagcaaattg ttccagaaat tcacccagaa
     2821 aaagtcaaag gagcaaatat cacatttgag catgatactt tcactatagg cgcaaattct
     2881 agctttgatt tgaatgcggt tataaatgtt ggagaggcca aaaacaaaaa taaatttgta
     2941 gaatcattta ttcattttga gtcagtggaa gaaatggaag ctctaaactc caacgggaag
     3001 aaaataaact tccaaccttc tttgtcgatg cctctaatgg gatttgctgg gaattggaac
     3061 cacgaaccaa tccttgataa atgggcttgg gaagaagggt caagatcaaa aacactggga
     3121 ggttatgatg atgatggtaa accgaaaatt ccaggaacct taaataaggg aattggtgga
     3181 gaacatggta tagataaatt taatccagca ggagttatac aaaatagaaa agataaaaat
     3241 acaacatccc tggatcaaaa tccagaatta tttgctttca ataaccaagg gatcaacgct
     3301 ccatcatcaa gtggttctaa gattgctaac atttatcctt tagattcaaa tggaaatcct
     3361 caagatgctc aacttgaaag aggattaaca ccttctccac ttgtattaag aagtgcagaa
     3421 gaaggattga tttcaatagt aaatacaaat aaagagggag aaaatcaaag agacttaaaa
     3481 gtcatttcga gagaacactt tattagagga attttaaatt ctaaaagcaa tgatgcaaag
     3541 ggaatcaaat catctaaact aaaagtttgg ggtgacttga agtgggatgg actcatctat
     3601 aatcctagag gtagagaaga aaatgcacca gaaagtaagg ataatcaaga tcctgctact
     3661 aagataagag gtcaatttga accgattgcg gaaggtcaat atttctataa atttaaatat
     3721 agattaacta aagattaccc atggcaggtt tcctatattc ctgtaaaaat tgataacacc
     3781 gcccctaaga ttgtttcggt tgatttttca aatcctgaaa aaattaagtt gattacaaag
     3841 gatacttatc ataaggtaaa agatcagtat aagaatgaaa cgctatttgc gagagatcaa
     3901 aaagaacatc ctgaaaaatt tgacgagatt gcgaacgaag tttggtatgc tggcgccgct
     3961 cttgttaatg aagatggaga ggttgaaaaa aatcttgaag taacttacgc aggtgagggt
     4021 caaggaagaa atagaaaact tgataaagac ggaaatacca tttatgaaat taaaggtgcg
     4081 ggagatttaa ggggaaaaat cattgaagtc attgcattag atggttctag caatttcaca
     4141 aagattcata gaattaaatt tgctaatcat gctgatgaaa aggggatgat ttcttattac
     4201 ctagtagatc ctgacaaaga tgcttctaaa tatgaaaaac ttggtgaaat ttctgaagat
     4261 aaactcaaaa atgcaaagag cccagaggaa aataccaata ataatcaagc taaggatgaa
     4321 gattcaaaac cagatgaaaa aagttcagtt gagggagaag ctagccttga aataaataaa
     4381 actatttcaa caattagaga gtttgaaaat aaagacctaa agaaactcat taaaaagaaa
     4441 tttagagaag ttgatgattt tacaagtgaa actggtaaga gaatagagga atacgattat
     4501 aaatacgatg ataagggaaa tattattgct tatgacgatg gtagtgcctt acaatatgaa
     4561 actgaaaaac ttgacgaaat aaaatcaaaa atttatggtg ttctaagccc atctaaagat
     4621 ggacactttg aaattcttgg aaagataagc aatgtttcta aaaatgccaa ggtatattat
     4681 ggaaatagct ataaatcgat agaaatcaaa gcgactaagt atgattcaca ttcaaaaacg
     4741 atgatatttg atttatacgc taatattaat gatattgtag atggattagc ttttgctgga
     4801 gatatgagat tatttgttaa agatgacaat caaataaaag ctgaaactaa gattagaatg
     4861 cctgaaaaaa ataaggaaac taaagcagaa tatccctatg tatcaagtta tgggaatgta
     4921 atcgaattag gagaaggaga tctttcaaag aacaaaccag acaatttaac taaaatggaa
     4981 tctggtaaaa tctattctga ttcagaaaaa caacaatatc tgttaaagga taacatcatt
     5041 ctaagaaaag gctatgcact aaaagtgact acctataatc ctggaaaaac ggatatgtta
     5101 gaaggaaatg gagtctatag caaggaagat atagcaaaaa tacaaaaggc caatcctaat
     5161 ctaagagtcc tttcagaaac aacaatttat gctgatagta gaaatgttga agatggaaga
     5221 agtacccaag ctgtattaat gtcggctttg gacggcttta acattataag gtatcaagtg
     5281 tttacattta aaatgaacga taaaggggaa gctatcgata aagacggaaa tcttgtgaca
     5341 gattcttcta aacttgtatt atttggtaag gatgataaag aatacactgg agaggataag
     5401 tccaatgtag aagctataaa agaagatggc tccatgttat ttattgatac caaaccagta
     5461 aacctttcaa tggataagaa ctactttaat ccatctaaat ctaataaaat ttatgtacga
     5521 aatccagaat tttatttaag aggtaagatt tctgataagg gtggttttaa ctgggaattg
     5581 agagttaatg aatcggttgt agataattat ttaatctacg gagatttaca cattgataac
     5641 actagagatt ttaatattaa gctgaatgtt aaagacggtg acatcatgga ctggggaatg
     5701 aaagactata aagcaaacgg atttccagat aaggtaacag atatggatgg aaatgtttat
     5761 cttcaaactg gctatagcga tttgaatgct aaagcagttg gagtccacta tcagttttta
     5821 tatgataatg ttaaacccga agtaaacatt gatcctaagg gaaatactag tatcgaatat
     5881 gctgatggaa aatctgtagt ctttaacatc aatgataaaa gaaataatgg attcgatggt
     5941 gagattcaag aacaacatat ttatgtaaat ggaaaagaat atacatcatt tgatgatatt
     6001 aaacaaataa cagacaagac actaaacatt aagattgttg taaaagattt tgcaagaaat
     6061 acaaccgtaa aagaattcat tttaaataaa gatacgggag aggtaagtga attaaaacct
     6121 catagggtaa ctgtgaccat tcaaaatgga aaagaaatga gttcaacgat agtgtcggaa
     6181 gaagatttta ttttacctgt ttataagggt gaattagaaa aaggatacca atttgatggt
     6241 tgggaaattt ctggtttcga aggtaaaaaa gacgctggct atgttattaa tctatcaaaa
     6301 gataccttta taaaacctgt attcaagaaa atagaggaga aaaaggagga agaaaataaa
     6361 cctacttttg atgtatcgaa aaagaaagat aacccacaag taaaccatag tcaattaaat
     6421 gaaagtcaca gaaaagagga tttacaaaga gaagatcatt cacaaaaatc tgattcaact
     6481 aaggatgtta cagctacagt tcttgataaa aacaatatca gtagtaaatc aactactaac
     6541 aatcctaata agttgccaaa aactggaaca gcaagcggag cccagacact attagctgcc
     6601 ggaataatgt ttatagtagg aatttttctt ggattgaaga aaaaaaatca agattaagac
     6661 aaaagctata gaaaaaaatg gtttatgtac tgagattaga tagtgaggtg atgacatagt
     6721 tttgtgaaaa cagccattta taactcaatt atttagttta cttcactatt gatactattt
     6781 ggagttatta atggacttag tttagataac taatgaattg attgaaaggg ttagtattga
     6841 caatattggt catattgatt agaaaataga gtctatcaaa atttaaaggc taatagaggt
     6901 gatgagacaa tttcggctct ttgtcaactg tagtgggttg aagtcagcta agctcgagaa
     6961 aggacaaatt ttgtcctttc ttttttgat
//
LOCUS       DAVBBS010000082         6830 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVBBS010000082 DAVBBS010000000
VERSION     DAVBBS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6830
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.082"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(201..1625)
                     /locus_tag="VQU32_001966"
     CDS             complement(201..1625)
                     /locus_tag="VQU32_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HET2473362.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMLKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2169..4448)
                     /gene="pulA"
                     /locus_tag="VQU32_001967"
     CDS             complement(2169..4448)
                     /gene="pulA"
                     /locus_tag="VQU32_001967"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HET2473363.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4560..6518)
                     /gene="ligA"
                     /locus_tag="VQU32_001968"
     CDS             complement(4560..6518)
                     /gene="ligA"
                     /locus_tag="VQU32_001968"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET2473364.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6611..>6830)
                     /locus_tag="VQU32_001969"
     CDS             complement(6611..>6830)
                     /locus_tag="VQU32_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2473365.1"
                     /translation="FTQVQTKVESEFLGRVLSTIFTLAILFMPIAKGFMTVLPSVHLS
                     SFLIIGSGVIILSCISFIYVRTHFEKLI"
BASE COUNT         2020 a         1502 c         1238 g         2070 t
ORIGIN      
        1 actatatata gtaaaatgaa ataataacag gacaaatcga tcaggacagt caaatcgatt
       61 tctaacaatg ttttagaagt aaatctgtac tattctagtt tcaatatact ataataccaa
      121 aaagttcgta ttttttctat aattattatt attttatagc aaaaaataaa accaggaaaa
      181 caatttcctg attttacacg ttatttcaca tcaaaaacaa tggatttgac atttgtcatc
      241 gcttcaatgc tatatttaat tccttgcact ccagctccag aacctttgac accaaggaat
      301 gggaaattat ctggaccacg ctgggtttta ttattaatgt ggactgtacc tacttcaagt
      361 ttttcagcaa tttcaaaggc ttttttgaaa tcatttgtaa agactgatga ttgaaggccg
      421 aattcagatt cgttggcaaa ggcaatagct tcctctacac tagccacacg aatgattggt
      481 aaaacaggac caaatggctc ttcccatgcc actttcatat cttttgtaac ttggtcaaaa
      541 agcactggcc agaggagatt gccctcacgt ttgattggtg taagagcctg agcctctttt
      601 tcttgtgcat cctcaatcaa gccccaaatg aagtcggctg aagcattgtc aataacaggt
      661 gtaatatcag cattgtcaaa tggatcacca actgttaatt tagaaacttc ttcctgaagc
      721 aaagtagcta atttatctgc tacactttcg agaacaatga cacgtttaat ggccgtgcaa
      781 cgttgtcctg agtagctaaa ggctcccgca acaatttgct tggcagcatg ttccaaatct
      841 gcatcttcta gtacaagagc tgcatctttc ccaccaagtt ccaacatgat aggacgcata
      901 ccagctaaac gaccaatacg ttctccaata ggagttgaac ctgtaaagtt gatgaagttg
      961 acttctttgt gctcaatgat ataatcccca atttctgaac cacgacctgt aatggtgttg
     1021 aaaacacctg ccggaatccc tgcttcttca aatgctttag ccaacaagag tccagaaatg
     1081 gaaccttgtg ttggtggctt caacatgacc acattccctg caatcaaggc aggtgcaatt
     1141 ttagaagcag ataaattaac tggataatta aagggagcaa tcgctagcac gataccaact
     1201 ggttcacgac ggacaacagc cagtttgttt ttacttgctg cctcaaaacc accaccttcc
     1261 attgcttgtc cagtgatacg gagaccttcc tcagcagcat aacgaatcaa gtctgctgta
     1321 cgcactactt ctccaattgc tgctttaatc ccttttgcta cttctttggc aaggatagta
     1381 ccaatttttt ccttatcgcg ttctaaaata gctgctgttt tatgcaaata agccgcacgt
     1441 tcaattgctg ataaagctcg ccatgctggc agggctgcac gcgcagcttg catagcctca
     1501 tcagcttcag tctgagtcat ggctggaact gtacccaatt cttcttgatt gattggtgaa
     1561 taaatcgtaa tttcttgttc agatgatttc cattttccat ttactaaatt ctgatatctt
     1621 gtcaaattct tgtcctcctg aaaatgatta agatatattt taccaaattt tcagaaaata
     1681 acaacccttt ttgcaaaatt tttgagatta ttttcacaag cttgattttt caaagtatac
     1741 tcaataaaaa taaaaaaatc cactacgtca aggcgaggct aatgtggttt gaagaaattt
     1801 tcgaagagcg tgaatgagta tcatctatag taaaataaaa aaactgaaca atttggttgg
     1861 ggacagccaa accaatttct cacaatgttt cagaaacaag ggtgtgctat tccaatttca
     1921 gcctactata actgtcatag attgctgaaa caaagtctag gtaaaagtct tcataataaa
     1981 aagacctcct atcaaatgtt caaaaacttt gataggaggt cttgtttcgt gaaaatattt
     2041 atcaaatttt ctatacaagt gagctgttag ccaggttctt tctattcttt caatttcaat
     2101 gaatggattt tttaccaata ctcataactg ggaatttttc tgtctaaaaa tagcgagata
     2161 gatggtattt ataaaacact caagacagct agactaatat catttaaaac attatcttct
     2221 tttgagcgac tgttggttac caacatagct aaatttcctg cattttcaaa ttgatagggt
     2281 tctgatttag cattcacaac taccaagagg tgttctttgc cgtgaacttc atagataagg
     2341 cagccgctat gttcaatcgc agaatgcaca aagacatgat ggtaaatttc atcatagcta
     2401 gagtaagaaa aggcaccagt ttttgtcttc aatcggatga cttgacggat aaactcaata
     2461 ctgtcttgac gctcattaat caagttccag ttcacttggt tcacactgtc aggagcatta
     2521 tagctattca tcgcacgctc tctatcatca tgggtcaact caccattttc accagtcgca
     2581 accagtttgg tacgaccaaa ttcttgaccg atttccataa aggccatccc ctgcatgagc
     2641 agattcatgg ctgtggcagt ttcgaccttg cgcatgattt gctctgaact ttggtctgga
     2701 tgaagggttg ccaataaatc gtgaagattg taattgtcat gggcttctac atagttaagc
     2761 acctgatttg gatgtgtata gcttcctaat tcacgacttc ctaggattgc tttagctaga
     2821 attggctctg tcgcagcacc actgacaaaa cctgacttga tagcaccata aacttctccc
     2881 cctttgacag catcgcgctg attgtcatta aagaaaccaa tatttggcat ctggtaggca
     2941 ttgtccttct tggccttatc ataaggggca agacctgttc ccatatccca tccttctcca
     3001 tagaggataa tgttggagtc aatttcatcc aagctttgac gaatcatctg catggtcttg
     3061 acatcatgaa tccccatcaa gtcaaaacgg aagccgtcaa tattatattc ctgcacccag
     3121 tatagaagag aatcaatcat atacttgcga aacatttcgt gttcactggc tgtttcattt
     3181 ccaacacccg ttccattctg gaaggtacca tctggattca tacgataata gtaatcaggg
     3241 actgttgttt ggaatggtgc atcaacaact gagaaggtat ggttatagac tacatccata
     3301 atgactccaa tacccgcatc gtgataagct tgaaccatca ccttcaaatc acgaatgacc
     3361 tgagctggat catctggatt agttgaaaaa ctagtttctg gcgcgttata gttttgtgga
     3421 tcataacccc agttgtaggt tacatttcca tcctcatcgt attctttatg acggtctgca
     3481 attggttgca attgaacata attgtagccc agcttcttga tgtaatcaaa agcagttgac
     3541 tggccgtatt ggttaactgt tccagcctga gcagcaccca agaaagttcc tcgaagatgt
     3601 tcatctacac ccgatgtagg tgatttagtc aaatcacgaa tatgcatttc acagataact
     3661 gccttacatg gattttccaa gcgccaagta gcctccgaac cgtgcttaac ctcgaagttt
     3721 tcaacttgct tttctacatg gctcagaata gctgaacgtt tgccatcagg gctggtcgcg
     3781 attgtataag gatcacgtgt cagtgtttgg tgatgaggga attggacttg atactgataa
     3841 gtcttaccta ccaaatcttc ttcaacatcc aaactccaga caccgattgt attgtcctta
     3901 tgattataag agtagctatt gcctcttttc atctcaaaag tcttccaaac gggtgcatca
     3961 ttagcagctg attcataaac gacaacttgc acttctgtcg ctgtaggtga ccagagggaa
     4021 aaatgagcct gattgtcctc tacacggcaa cccaattctc cttggtaacc ccaatgatga
     4081 tcaaaactag cactgttaat ggccttatca aaggcaaaag gattttgatt tttatagaaa
     4141 ggactggcaa tagcaggatt ttcagagtaa taaatcctat catcgccttc caaaatccag
     4201 acctctgtta ataggggata gtgattaaaa cggatagaat attctttact agtttgacct
     4261 gtatgaacca caaaattcaa gctttctata acatgtgaac ttgggtgttc aaagctaaat
     4321 aaagctccaa aataatcttc tttgtaggtt agcaaatcaa ttcgttgatc ctgacttttt
     4381 acaaaggagc aagtgtcata ttctccattc ttacgatggt aatgaatgcg catagggtag
     4441 ttatacattt tttatttttc ctttttactt tgtttctatt tcactaataa atttttgtca
     4501 atctcgtctc aattaacaga catagtcata ttctctaaac tctgttttta aacgatccat
     4561 tacaaacttt ctagccatgc ctcatctctg acctggatac caagttcttg tgctttttgc
     4621 agtttacttc cagcgtctgc acctaccacg acgaggtcgg tctttttaga aacactacct
     4681 gtcactttgg cacccagact ttcgagttta cttttagctt ctgagcgctt gagtcgttcc
     4741 aattttcctg tcaataccac ggtcaaacct gacaaggccg catccgctac taccgtctgt
     4801 cctttatagt ccagattgac cccagtttct ttcaattctc tgagcagaat ttcagagcct
     4861 tctgtcgcaa aataagtctg aagacttttg gcaatcacgc cacctagact ttcaatacta
     4921 gccacttcct ctgaatctgc ctgagacaga ttttcaattg aatggaaata ttgaagtaaa
     4981 agctgactag ccttgcttcc gacatgacga attcccaaac caaataagag cttctcggca
     5041 gaattttcct ttgatgcttg gatagcctga tacagtttag cagcggactt ttccttaacc
     5101 ccctctaaaa ggaggaaatc ctcttcttgc aaacgataaa tatccgccac atccttgact
     5161 aaattagcag caaaaagctt ctcaacaata gatggaccaa ggcctgtaat attcatagca
     5221 tcacgagaag caaagtgaat caagccttcc atgatttgag cagggcaacg cggattgata
     5281 caacgtaggg ccacttcatc ttcaaagtgc aacaagtcag agttacaact tggacagttt
     5341 gtagggatat ctagtttttc ttcagaaacc cgtttggact ctaccacacg taaaacggca
     5401 gggatgatgt caccagcctt atatacaatg accgtatcgt cttttcggat atctttttca
     5461 gcaatataat ctacattgtg cagggtcgca cggctaacag tcgtaccggc aagttgtact
     5521 ggtgttagat tagcagttgg agttacaaca ccggtacggc caactgtcca gtcaactgat
     5581 aagagttgag cttctttttc ttcagcaggg aacttgtagg ctactgccca ctttggagcc
     5641 ttaacggtaa aaccaagttc ttcttgactt gctaggtcgt tgaccttgat taccactcca
     5701 tcaatatcgt aaggcagatt ttcccgttcc tgtcctactt cttggataaa attccagatt
     5761 tcatctatgt tttcagccaa gattcgctta ggattgacca caaaacctag ttgttctagg
     5821 tacttcaaac ccttttcttg gctatcacga gttgaagggc tggcttcttg atagagaaac
     5881 gttgcaagat tacgcttggc aactactgct gtatccaact gacgcagagt tcctgctgcc
     5941 gcattacgag gattagcaaa ttcaggctct ccattttctt ggcgcacttg gttaacttgg
     6001 tcaaaggaag cgcgtggcat gtaacattcc ccacgaactg tgatatctag ttcttctggc
     6061 aaagtcaaag ggatgtcctt aacacgcttg aggttttctg tgatattttc accaattgaa
     6121 ccatctccac gtgttacccc agcaaccaaa atcccctttt cataagtcag cgagatagat
     6181 aagccatcga ttttcagctc acaaatatag gtcggatgag ccacttcctt acgaacacgc
     6241 gcatcaaaag catctagctc ctcacgtgaa aaagcatcct gcaaactata aagaggatac
     6301 tgatgactgt atttttcaaa accatctaaa accttgccac caacacgatg agtcggactg
     6361 tctgctagca cttgctctgg ataagcagtt tctaactcga ccaactcacg gtaaaggcgg
     6421 tcatactcac tgtctgaaac cgagggatta tcgctggtat agtactcagt cgcatagcga
     6481 ttgagcaaag cgactaactc attcattctt ttattcataa gaccatttta ccataaaaca
     6541 agccctcctc acaaacgaga agggcggaaa aaacacttag tttgaaatta tttttgaaac
     6601 tcaagcaacc ttatatcaat ttttcaaaat gagttcgaac ataaataaac gatatacaag
     6661 acaagatgat aacaccactt ccaattatca ggaaagaaga gagatgtaca cttggcaaga
     6721 ctgtcataaa tccttttgca ataggcataa atagaatagc taaggtaaaa attgtactca
     6781 gtactcttcc aagaaattcg ctctcaacct tggtttgtac ttgagtaaaa
//
LOCUS       DAVBBS010000083         6697 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVBBS010000083 DAVBBS010000000
VERSION     DAVBBS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6697
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.083"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(45..314)
                     /locus_tag="VQU32_001970"
                     /pseudo
     CDS             complement(45..314)
                     /locus_tag="VQU32_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            506..2272
                     /gene="patB"
                     /locus_tag="VQU32_001971"
     CDS             506..2272
                     /gene="patB"
                     /locus_tag="VQU32_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HET2473366.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTHVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            2411..3100
                     /locus_tag="VQU32_001972"
     CDS             2411..3100
                     /locus_tag="VQU32_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET2473367.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLDEL"
     gene            3104..3283
                     /locus_tag="VQU32_001973"
     CDS             3104..3283
                     /locus_tag="VQU32_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473368.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            3270..4619
                     /locus_tag="VQU32_001974"
     CDS             3270..4619
                     /locus_tag="VQU32_001974"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET2473369.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            4743..6203
                     /locus_tag="VQU32_001975"
     CDS             4743..6203
                     /locus_tag="VQU32_001975"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET2473370.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            6217..6651
                     /locus_tag="VQU32_001976"
     CDS             6217..6651
                     /locus_tag="VQU32_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET2473371.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNTYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT         2097 a         1294 c         1445 g         1861 t
ORIGIN      
        1 gcaggtgaaa agtgagggca tgctttggca gcttgtatga gtacctaccg cagatgaggg
       61 gaaccccgtt tgaccattct tccagctaaa tcaatctgac ctgactgata aatagaagaa
      121 tccagtccag cgaaagcttg taattgagca ggattatcaa aggcatgaat atttcgaatc
      181 tcggctaaaa tgaccgcccc taaacgattc ccaatcccag taaccgtcgt gatgaccgag
      241 ttgaactcag ccatcaagtc attgacacat ttttccgcct tgtcaatgag cctcttgtaa
      301 tgtttgatga tttcattaca cgagataaaa cgtctatgcg ttatcaaact cattaccaat
      361 taaaacaaaa agctgtggtt agatcctttc ggaaatcgtc aagcgattgg aggaaatgaa
      421 ctaatccaca gcggcttatt ccaagtatac cacttgggct ttggcagtag ctaactgcgc
      481 taaatataat ataaggagga gtaaaatgaa gacagttcaa tttttttggc attattttaa
      541 ggtctacaag ttctcatttg tagttgtcat cctgatgatt gttctggcga cttttgccca
      601 agccctcttt ccagtctttt ctggacaagc ggtgacgcag ctagccaatt tagttcaagc
      661 ttatcaaaat ggcaatccag aacttgtatg gcaaagccta tcaggaatca tggtcaatct
      721 tggcctgctg gttttggttc tatttatctc tagtgtaata tacatgtgtc tcatgacgca
      781 cgtgattgca gaatcgacca acgagatgcg caaaggcctc tttggtaagc ttgctcagtt
      841 gacggtttct ttctttgacc gtcgacaaga tggcgatatc ttgtctcatt ttaccagtga
      901 tttggataat atcctccaag cctttaacga aagcttgatt caggtcatga gcaatattgt
      961 tttatacatt ggtctgattc ttgtcatgtt ttcgagaaat gtgacgctgg ctctcatcac
     1021 cattgccagc accccattgg ctttccttat gctgattttc atcgtgaaaa tggcacgcaa
     1081 atacaccaac ctccagcaga aagaggtagg gaagctcaac gcctatatgg atgagagcat
     1141 ctcaggccaa aaagccgtga ttgtgcaagg aattcaagag gatatgatgg caggatttct
     1201 tgaacaaaat gagcgcgtgc gcaaggcaac ctttaaagga agaatgttct caggaattct
     1261 tttccctgtc atgaatggga tgagcctgat taatacagcc atcgtcatct ttgctggttc
     1321 ggctgtactt ttgaatgata agtctattga aacaagtaca gccctaggtt tgattgttat
     1381 gtttgcacaa ttttcacagc agtactacca gcctattatc caagttgcag cgagttgggg
     1441 aagccttcag ttggccttta ctggagctga acgaattcag gaaatgtttg atgcagagga
     1501 ggaaatccga cctgaaaagg ctccaacctt cactaagttg caagaaagtg ttgaaatcag
     1561 tcatatcgat ttttcatact tgcctgataa acctattttg aaagatgtca gcatttctgc
     1621 ccctaaaggc cagatgacag cagttgttgg gccgacaggt tcaggaaaaa cgactattat
     1681 gaacctcatc aatcgctttt atgatgttga tgctggtggt atttattttg atggtaaaga
     1741 cattcgtggc tatgacttag atagtcttag aagcaaggtg ggaattgtat tgcaagattc
     1801 ggtcttgttt agcggaacga ttcgagacaa tatccgattt ggtgtgccag atgctagtca
     1861 ggaaatggtt gaggtagcag caaaagcaac ccacattcac gactatatcg aaagtttgcc
     1921 tgataagtac gatactctta ttgatgatga ccagagcatc ttttcaacag ggcagaagca
     1981 attgatttca atcgctcgaa ccctgatgac agatccagaa gttctcattc tcgatgaagc
     2041 aacttcaaac gtagatacgg tgacagaaag caagattcag catgccatgg aggtggttgt
     2101 agcaggtaga actagtttcg tcattgccca ccgcttgaaa accattctca atgcagatca
     2161 gattattgtc cttaaagatg gagaagtcat tgaacgtggt aaccaccatg aacttttgaa
     2221 gctaggtggc ttttattcag aactctatca caatcaattt gttttcgaat aagaaagaag
     2281 ttgtcctatg tgggcagctt tttcttgtcc ataaaaaatg tttatcacag ccttaaaaaa
     2341 aacatattag acgaaagtca ttttgagtga tatgatagga ctatcgttag cattcgaaag
     2401 gagaggcatc atggctagaa cggttgtagg agttgctgca aatctatgtc ccgtagacgc
     2461 agaaggcaaa atcattcatt catctgtatc ttgtagattc gcagagatca ttcgtcaagt
     2521 cggtggtctc cctttagtca ttcctgttgg tgatgagtca gttgtacgtg attatgtgga
     2581 aatgattgac aaactcattt tgacaggagg ccaaaatgtt catcctcagt tttatggaga
     2641 gaaaaagacc gtcgagagcg atgattacaa tctggtccgt gacgaatttg aattggcact
     2701 cttgaaggaa gcgcttcgtc agaataaacc aattatggca atctgtcgcg gtgtccaact
     2761 tgtcaatgtt gcctttggtg gaaccctcaa tcaagaaatc gaaggtcact ggcaaggact
     2821 acctttcggg acatctcact ctattgagac agtagaagga agcgtggtgg ctaagctatt
     2881 tgggaaagaa agtcaggtca actcagtcca tcgtcaaagc attaaagatt tggcacctaa
     2941 tttccgtgta actgctattg attcaagaga ccaaactatt gaagcgattg agtctatcga
     3001 cgagcaccgc attatcggtt tacagtggca tccagagttt ctggttaatg aagaagatgg
     3061 caatttagaa ttatttgagt atttattgga tgaactgtaa caattggaac atctggtcgt
     3121 tctttctttt attttttcaa aatttttaga atgtggtatt ttacgcaaac gtttgaattc
     3181 tgataaaaat tggagaaatt cgacaaaaaa acttgaaaaa aacgaaggta agcgttatga
     3241 tagaaaagaa gaaatattgg aggaagaaca tgtcacatat taaatttgat tattcaaaag
     3301 ttttagacaa atttgttgca ccacatgaag tggaatacat gcaatcacaa gtaacagcag
     3361 cagatgaatt gatccgtaaa ggaactggtg ctggtagcga cttcttggga tggttggacc
     3421 ttcctgaaaa atacgaccgc gaagaatttg accgcatctt gaaagctgct gagcaaatca
     3481 agtcagacag cgatgttttg gttgtgatcg gtattggtgg atcttacctt ggtgcaaaag
     3541 cagcaatcga cttcttgaat caccattttg ctaacttgca aacaaaagaa gaacgcaaag
     3601 ctccacaaat cctttacgca ggaaactcaa tctcatctac ttaccttgct gacttggtag
     3661 agtatgttgc agacaaagac ttctcagtaa acgtaatttc taaatcaggt acaacaactg
     3721 aaccagcgat tgctttccgt gtctttaaag aactcttggt taagaaatac ggtcaagaag
     3781 aagctaacaa acgtatctat gcaacaactg accgccaaaa gggtgctgtt aaggttgaag
     3841 cagacgctaa cggttgggga acatttgttg ttccagatga tatcggtgga cgcttctcag
     3901 tattgacagc cgttggtttg cttccaatcg cagcatcagg agctgacata aaagctctta
     3961 tggaaggtgc gaatgcagct cgcaaagact acacttcaga caaaatctct gaaaacgaag
     4021 cttaccaata cgcagctgtt cgtaacatcc tttatcgtaa aggctatgca actgagatct
     4081 tggtaaacta tgagccatca cttcaatact tctcagaatg gtggaaacaa ttggctggtg
     4141 aatcagaagg aaaagaccaa aaaggtatct acccaacttc agccaacttc tcaactgact
     4201 tgcactcact tggtcaattt atccaagaag gaactcgtat catgtttgaa acagttgtcc
     4261 gtgttgacaa acctcgtaaa aacgtgctta ttcctacttt ggaagaagac cttgacggac
     4321 ttggttacct tcaaggaaaa gacgttgact ttgtaaacaa aaaagcaact gacggtgttc
     4381 ttcttgccca cacagatggt gatgtaccaa acatgtatgt gactcttcca gagcaagacg
     4441 ctttcactct tggttacact atctacttct tcgaattggc aattgccctt tcaggttact
     4501 tgaatgctat caacccattt gaccaaccag gtgttgaagc ttataaacgt aacatgtttg
     4561 cccttcttgg aaaaccagga tttgaagaat tgagcaaaga acttaacgca cgtctataat
     4621 agaagaaaag agtggtttgc ccactctttt tactctcttt atccatagaa attggactca
     4681 gccaagactt gtgatataat atagaaagca aaaaggcaga cgcctagata ataggagaaa
     4741 ctatgtcaaa agatatccgc gtacgttacg caccaagtcc aacaggacta ctacacatag
     4801 gaaatgctcg tacagcattg tttaattact tgtatgcgcg ccatcatggt ggaacatttc
     4861 tcatccgtat cgaagatact gaccgtaaac gccatgtcga ggatggtgaa cgttcacaac
     4921 ttgaaaacct tcgctggtta ggcatggatt gggatgaaag tccagaatca catgagaatt
     4981 atcgccagtc tgagcgtttg gacttgtatc aaaaatacat cgaccaacta ttagctgaag
     5041 gaaaagccta caaatcttac gttacagaag aagagttggc agctgaacgt gaacgtcaag
     5101 aagtagctgg cgaaacacca cgctacatca atgaatacct tggtatgagt gaagaagaaa
     5161 aagcagctta catcgcagaa cgtgaagcag cagggatcat cccaactgtt cgtttggctg
     5221 tcaatgagtc aggtatctac aagtggcatg atatggtcaa aggcgatatc gaatttgaag
     5281 gtggcaatat cggtggtgac tgggttatcc aaaagaaaga cggttaccca acttacaact
     5341 ttgccgttgt tatcgatgac cacgatatgc aaatctctca tgttatccgt ggagatgacc
     5401 atattgctaa tacaccaaaa cagcttatgg tctatgaagc tcttggttgg gaagctccag
     5461 agttcggtca catgaccttg attatcaact ctgagactgg taagaagtta tctaaacgtg
     5521 atactaatac acttcaattt atcgaagatt accgaaagaa aggttacctt ccagaagcag
     5581 tctttaattt tattgctctt cttggttgga acccaggtgg tgaagatgag attttctctc
     5641 gtgaagaact cattaaactt tttgatgaaa accgcctcag caagtcacca gcagcctttg
     5701 atcagaaaaa actagactgg atgagcaatg attatatcaa gaatgcagac cttgaaacca
     5761 tctttgaaat ggcaaaacca ttcttagagg aagcaggccg tttgactgac aaggctgaaa
     5821 aattagttga gctctataaa ccacaaatga aatcagtaga tgagattatc ccattgacag
     5881 atcttttctt ctcagatttc ccagaattga cagaagcaga gcgcgaagtc atgacgggtg
     5941 aaacagttcc aacagttctt gaagcattca aagcaaaact tgaagcgatg acagatgata
     6001 aatttgtgac agaaaatatc ttcccacaaa ttaaagcagt tcaaaaagaa acaggtatta
     6061 aagggaaaaa tcttttcatg cctattcgta tcgcagtttc aggcgaaatg catgggccag
     6121 aattaccaga tacaattttc ttgcttggac gtgaaaaatc aattcagcat atcgaaaaca
     6181 tgctaaaaga aatctctaaa taaagaggat acaataatgg acatctggga aaagatgtac
     6241 gaagaagcac agaaactata caatccacat gaagtatcag actttgttta tgccaatcat
     6301 gttgtagctg cagtagaagc agaagatgga caaatattta caggattctg tatggaggga
     6361 acctgtggtg ttttccatct ctgcgcagaa cgggcggccc tcttcaatac gtaccaattt
     6421 tcaggacaaa ctaaggttaa gaaagtatta gcctttcgag acaaaccacc ttatggtgga
     6481 agttcagcca tgccttgcgg tgcttgtaga gaattccttt tagagcttaa tgctgaaaat
     6541 aaagatgcag aattcatgat ggactataat ataagaaaaa cagttaaagt cgcagaacta
     6601 atcccttatt ggtggggaga agaacgtgct tctaagttta atgagcgata gaagagtcag
     6661 tataattctg gcttttttta ctataagttg aaaaaat
//
LOCUS       DAVBBS010000084         6635 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVBBS010000084 DAVBBS010000000
VERSION     DAVBBS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.084"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(84..1244)
                     /locus_tag="VQU32_001977"
     CDS             complement(84..1244)
                     /locus_tag="VQU32_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2473372.1"
                     /translation="MKKKFALSFVALASVALLAACVEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTNMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            complement(1345..1593)
                     /locus_tag="VQU32_001978"
     CDS             complement(1345..1593)
                     /locus_tag="VQU32_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HET2473373.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            complement(1672..2262)
                     /gene="clpP"
                     /locus_tag="VQU32_001979"
     CDS             complement(1672..2262)
                     /gene="clpP"
                     /locus_tag="VQU32_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HET2473374.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            complement(2436..3065)
                     /gene="upp"
                     /locus_tag="VQU32_001980"
     CDS             complement(2436..3065)
                     /gene="upp"
                     /locus_tag="VQU32_001980"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET2473375.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETFQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(3154..3621)
                     /locus_tag="VQU32_001981"
     CDS             complement(3154..3621)
                     /locus_tag="VQU32_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HET2473376.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            complement(3640..4197)
                     /locus_tag="VQU32_001982"
     CDS             complement(3640..4197)
                     /locus_tag="VQU32_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET2473377.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            4322..5167
                     /locus_tag="VQU32_001983"
     CDS             4322..5167
                     /locus_tag="VQU32_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2473378.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEDGGLLM
                     GYEID"
     gene            5226..6398
                     /locus_tag="VQU32_001984"
     CDS             5226..6398
                     /locus_tag="VQU32_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET2473379.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSNPI"
     gene            6449..>6635
                     /locus_tag="VQU32_001985"
     CDS             6449..>6635
                     /locus_tag="VQU32_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET2473380.1"
                     /translation="MEHIILLRGVTPNGKNAIPKMSYLVDILTEADFQQVRTYIQSGN
                     IILESNLALEEIREQVHT"
BASE COUNT         1893 a         1385 c         1287 g         2070 t
ORIGIN      
        1 agtttgaaga gattttcgaa gagtattaaa tatcgaaaca gggagtgagt caaaggctca
       61 ttccctattt caacattttt ctattatggt tttacaactt ctgctgcttc aactttacca
      121 ttgttcatgg tcatcatgta agcagttttg actgtgttgt ggtctgcatc gaagcttgtt
      181 tgaccagtta caccttcaaa atcttttgtt ttagcaaggt tatccttgat ttcacctgaa
      241 tttttagcac cttttgctgc gtttgctaca aggtgaactg aatcataagc caaggctgca
      301 aatgttgaag gctcttcatt gtacttagca cggtaagcgt caaggaaggc tttagcttta
      361 gctgaaactt ctacagtagt tgagaagcct gagataaagt agatgtttga tgctttttca
      421 gcagttgctt gttgtacaaa ctcctcaccg ttgaatccat caccaccaac gattggtttg
      481 tcaattccca tgccacgcgc ttggtttaca atcttaccag cctcattata gtaaccagga
      541 acaacgatag catcaaagtc tttccctttc atgtttgtaa gggctgcttg gaagtctgtg
      601 tcacctgcta cgaaagtttc atctgcaacg atttcaccct tgtatgactc gcggaaagat
      661 ttagcaatcc ctttagcata gtcactggca ttgtcagtgt aaagaacaac tttcttagca
      721 tttaattttt cagaaacata gtttgagata atttttcctt ggaagctatc ttggaaagtt
      781 ccaataaaga ggtaatcttg acctttagtc aatccatctt gagtcgcact tggtgagatc
      841 aatggaacac ctgcttttgt agcgttcgct accgcagctg cagtcgcacc agatgtcgca
      901 ggtcctacga ctgctgatac tttagattgg gttacaaggt tagttgtaac tgaagcagcc
      961 tcagctgttt cagacttatt atctttatcg actacttcga tttgttttcc atcgatacca
     1021 cctgcggcat tgatttcatc aacagccaat tgggcacctt tttgttcagc tgttccgtat
     1081 gcagctaaag aacctgattc ttcaaagtta aacccgattt taattgtctt ttcctctact
     1141 gagttaccag cagtgttgac tgctccagac ttcacttcta cacaggctgc aagaagtgct
     1201 acacttgcaa gcgccacaaa cgatagggca aattttttct tcatcttttt gtctccttat
     1261 ttcttaaata gtctgttaag aatataccga attatcagaa aattgtcaat gttttccttg
     1321 aactttttcg atgataacgt tttcctatcg ataaagattg cccacaaagg gtgtttccaa
     1381 ctcttggata tgacaaaccc taactttttt aataaatttt tccttgctca agcgtccgac
     1441 caattgctcc acttcttgag ttggaacata gagttgtaag taacgatgtt tcttggaatg
     1501 ataggtaata tctccataat cctgcagttt tttggcatca cgattatagt aaagatagat
     1561 aatcaagcca gagcgattga ctttttcaaa catgataaat cctctttcta agaaatctct
     1621 ccctattata ccaaaaaaag caaacccagt cgagtttgcc ttctatcatc attagttcaa
     1681 tgaattgttg gccataattt catcaataaa gccatattca agtgtttcct gggcgctcat
     1741 ccagttatca cgttctgcat ctgcatggac tttttccatt gactgacctg aattttcagc
     1801 caagattttt tccaaggtat tacgagtttt gagcaagtgt tctgcagcga tagccatatc
     1861 agtttgttgg gtaccaccac ctgtaccgcc cattggttgg tgaatcatgt attcagcatt
     1921 tggaagcatg aaacgtttgc cttttgctcc acttgatgcg atgactgttc ccatagatgc
     1981 agccattccc ataacaatgg tttggacatc tgccttgata aagttcatgg tatctacgat
     2041 tgccaaacca gctgaaacag aaccacctgg tgtattgaca taaaggtaaa tatcttttgt
     2101 actatcttgg gcatccaaga aaagcagttg ggcaataaca gagttagcca tattgtcttc
     2161 aaccggacct gtcagcataa tgatgcggtc tttgagaaga cgtgagtaaa tatcgtaaga
     2221 acgttctcca cggcttgttt gttcaataac tacaggaatc attcatttct ccttttgagt
     2281 tttaattttg ttggtcaaat gactgaagat aagactatta taatatcttg gtcaaaaaag
     2341 gtcaaatttt tgctctgctt tcattagaca gaaacaaaaa cccaacctcc tttcgtgact
     2401 ggaaatactt ttccaagtca ttcttctttt cgatcttatt ttgtaccgaa caagcggtct
     2461 ccagcatctc caagacctgg aacgatataa ccgtgttcgt tcaaacgttc atccaaggct
     2521 gctgtaaaga tttctacatc tggatgagct tcttgaaggg cttttacacc ctctggagca
     2581 gatacaaggc agacaaattt gatatttgat gcgccacgtt ttttaagaga atcaacagcc
     2641 aagattgctg agccacctgt tgccaacatt gggtctacta caaaaatttg acgttggtca
     2701 atgtcctcag gcaatttcac caagtattca actggttgaa atgtttcttc atcacggtac
     2761 ataccgatgt ggccaacttt agcagctgga accaagctca agagaccatc aaccatcccg
     2821 atacctgcac gcaagattgg gacgatggcc aatttcttac ctgccaattg tttttgaact
     2881 gtttttgtaa ttggtgtttc gatttccaca tcttctagtg gaagatcacg aagtacttca
     2941 taccccatca acattgcaat ctcatctact agctcacgaa aagcttttgt agaagtatct
     3001 gtacgacgca agattgacaa tttgtgttga atcagtgggt gattaataac ttcaattttt
     3061 cccatttttg gaattccttc tttcaattta ttcttcttat tataccaaaa aacgggttaa
     3121 aaatctttct aaaccattta tttttgataa tttttacatt agatcagcct ctttaagagc
     3181 tgtctgtact gtctcaagtg gtaaatgggt caattctgtc cctttttctt gataaaggta
     3241 ttgggcgtag tcgtccattc ggtactggtt gatataaacc acacgcttgc agccgacctg
     3301 aagcaattgt tttgtacagt tgagacaagg aaaatgggtt acataggctg taaagccttt
     3361 gggaacacca cgctcagcac cttgaaggat agcattgacc tcagcgtgaa gggtgcgaac
     3421 acagtggcct tcaatgacca aacattcgtg atcaatacaa tgctcagtcc ctgacaccga
     3481 accattgtaa ccagtggaaa taaccttatt atcttttacc agaatcgcgc ccactttagc
     3541 acgtttacaa gtggaacgat tcgcaattag tagagcttgg gctgcaaaat actcatccca
     3601 ggccagtctt ttttcagtca tctcttttct cctttttctc tattttttaa aaaatggtaa
     3661 acctaaatct gcaatctttt cagctggtac cttcatgcca tccttgatcc attttagaag
     3721 gacagagacg atggctgagc tccagaagga atgaagataa gagctgacac cttttgattt
     3781 cccatggtat ttttctagaa attcctgcat ggcttggaca aagatttttt ccagatggta
     3841 atccaaggcc aattgaatta ctctagcttc ctttctggcc tcccggaaaa ggtgaaccca
     3901 aaccaaataa aggtctgtct ttaaatcgta atgatgcagc tgttccataa tattgtggac
     3961 agttcgttta aagacgctct ctaaaatttc ctctttggag tcataattgc gataaaaggc
     4021 cgcacgcgaa acacctgcac gtttgaccaa ttcagaaata ctaatcttgg tcagttcctt
     4081 tttttccaag agttgcaaga gggctgtttc aatggcttct ctggttaata aattggattc
     4141 ttggtttgat tttctgagat tttcaagaga cttttcagag attctacgtt cagacataac
     4201 attttctttc tacttgtcac aacagacgga tgatgctttt gtttcaaggg ttttcatgat
     4261 ataattgtaa aaaaagacag aacctttgtc aatgtataac tgacaaagat ggagaatttc
     4321 tatgacttgg aagattattg ctgactctgg ttgtgattat cgtcaactgc caacaccagc
     4381 tattaacaca acctttgtaa gtgtcccctt aaccattcaa gtagctgatc aggtctttgt
     4441 tgatgacgcc agtctcgata ttgaccaaat gatggaaacc atgtatgcaa ctgcagaagc
     4501 ttcaaaatcg gcttgtccaa gcccagatga ctatttgcga gcatttgaag gtgccaaaaa
     4561 cattttccta gtaaccatca caggtaccct ttctggcagt cacaatagtg ctcaactagc
     4621 aaagaatatt tatctggaag accatcctga cactaagatt catgtgattg atagtttatc
     4681 tgctggtggt gaagttgacc tactcgtaga aaaattgaat gacttgattg accagggctt
     4741 gtcttttgaa gaagtggttg aagctatcac cgcctatcaa gaaaaaacta agttgctctt
     4801 tgtcctagcc aaagtcgata acttggtgaa gaacggccgt ttgagcaagc ttatcggtac
     4861 ggtcgttggc cttctcaaca ttcgtatggt cggaaaagct agtgaaactg gaactctcga
     4921 attgctacaa aaagcaaggg gatcaaagaa atcagttcaa gctgcctatg atgagttagt
     4981 aaaagctgga tatgctggtg gccgtattgt catggcccaa cgcaataacg aaaaatgttg
     5041 tcaacagctc tcagagcgaa tccgtgaaac cttcccacaa gcggatatta aaattctacc
     5101 aacctctggt ctctgcagtt tctatgcaga agatggcggt ttgctgatgg gctatgaaat
     5161 tgattaattg tattcttgac aagaggtgac catcatctct tgtttttttg tggtacaata
     5221 gagctatgaa acattttgat actattgtca tcggtggggg acctgctggt atgatggcta
     5281 cgatttccag tagcttttat ggacagaaaa ccctcctcat cgaaaaaaat cggaaacttg
     5341 gaaaaaaatt agctgggact ggtgggggac gttgcaatgt gaccaacaat ggtagcttag
     5401 acaacctgct agctggaatt cctggaaacg gacgctttct ttacagtgtt ttctcccagt
     5461 tcgataatca tgacatcatc aactttttta cagaaaatgg tgttaaactt aaggtcgaag
     5521 accacggacg cgtctttcca gccagtgaca agtctcggac tattatcgaa gctttggaaa
     5581 agaaaatcac cgaactaggt ggtcaagttg ctactcaaat agaaatcgtt tctgttaaaa
     5641 aagtagatga ccagtttgtc cttaagtcag cggatcaaac cttcacttgt gagaaactca
     5701 ttgtcacaac aggtggtaag tcttatcctt cgactggttc gactggtttt ggtcacgaga
     5761 ttgctcgcca ttttaagcat accatcaccg atcttgaggc tgctgaaagt cctttattaa
     5821 cagattttcc acataaagcc ttacaaggga tttctttgga cgatgtgacc ctaagttatg
     5881 gtaagcatgt catcactcat gatttactct ttacccactt tggtttgtca ggtcctgctg
     5941 ccctacgcat gtctagcttt gtcaaaggtg gggaggttct ctcactcgat gttttgcctc
     6001 aactttctga gaaggacttg gttacatttc tagaagaaaa tcgggaaaaa tccttgaaaa
     6061 acgctttaaa aaccttgtta ccagaacgct tggccgaatt ttttgtacaa ggatatcctg
     6121 aaaaagtcaa acaactgact gaaaaggaac gagaacaact tgtccagtcc attaaagaac
     6181 ttaaaattcc tgtaactgga aaaatgtccc ttgcaaagtc ctttgttacc aagggtggag
     6241 tcagtctcaa ggaaatcaat cctaaaaccc ttgaaagtaa gctggtacct ggcctccact
     6301 ttgcaggcga agttatggat atcaatgccc acacaggtgg ctttaacatc acttctgccc
     6361 tctgtaccgg ctgggtggcg ggatcaaatc caatctaaat atacattact aaatggaaca
     6421 atcccccaaa aaaaagaaag gaactacttt ggaacatatt atcttgctaa ggggagtcac
     6481 tcctaatgga aaaaatgcta tccctaaaat gtcttatcta gtagatatct tgacagaagc
     6541 tgattttcaa caagttcgaa cttatattca aagtggaaat attattcttg aaagtaactt
     6601 agctctcgaa gaaatacgag aacaggttca tactc
//
LOCUS       DAVBBS010000085         6565 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVBBS010000085 DAVBBS010000000
VERSION     DAVBBS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.085"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            53..1015
                     /locus_tag="VQU32_001986"
     CDS             53..1015
                     /locus_tag="VQU32_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET2473381.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYDDTSISVSSNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVIIFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVEPLRYE"
     gene            1192..2199
                     /gene="gap"
                     /locus_tag="VQU32_001987"
     CDS             1192..2199
                     /gene="gap"
                     /locus_tag="VQU32_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET2473382.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2266..3102)
                     /locus_tag="VQU32_001988"
     CDS             complement(2266..3102)
                     /locus_tag="VQU32_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2473383.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQYLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETYVSRLKQFSNKTSLARCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            3228..5423
                     /locus_tag="VQU32_001989"
     CDS             3228..5423
                     /locus_tag="VQU32_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET2473384.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMASQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVAAVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIAYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5476..5628
                     /gene="rpmG"
                     /locus_tag="VQU32_001990"
     CDS             5476..5628
                     /gene="rpmG"
                     /locus_tag="VQU32_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2473385.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5638..5814
                     /gene="secE"
                     /locus_tag="VQU32_001991"
     CDS             5638..5814
                     /gene="secE"
                     /locus_tag="VQU32_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET2473386.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            5870..6406
                     /gene="nusG"
                     /locus_tag="VQU32_001992"
     CDS             5870..6406
                     /gene="nusG"
                     /locus_tag="VQU32_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET2473387.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2045 a         1136 c         1438 g         1946 t
ORIGIN      
        1 cacggataca gttgggaaag aagctattaa acatttggag agtccttttg ggatgaacat
       61 gtcaaatatt actatagatg ggcaagaaat ggatctgagt caaacgatgc cttcttatgc
      121 tcaagtagtg agtctttatg atgatacaag tatctctgtt agtagtaatg agacggacaa
      181 agtgttggct ggctccctct atactgatac aaatgaacag ggattaacga ttccaagcag
      241 tttactaaaa aattggaatg aacagacagg aaaaaatttg acagctaatg atcttattgg
      301 caaatcagtc tcagccagca ttgtagaaag tgctgccgaa actagtaaga ttgctcaatt
      361 tcaaacgaag attgtacgtg taatcaatga tgaagatgac atggaggaca gcaacagttt
      421 catgctgtct catcaaatgg aaacgatttt gaaagaggct ggatttacga aagctgtatc
      481 ttattttatc ttggaactca aagatccatc acagacaaaa gtagtaacag aagaattaca
      541 gaaaaataag aagtatactg tgctttctca acagagagtt cttgatattg tgattatctt
      601 tattcgtgtt attcagggat tattgattgt gctttcatca caagctattg tggtagcagc
      661 ggttatgatt ggtatcatta tttacatcaa tatcatgcag cgttccaagg aaataggtgt
      721 catgaaggca gttggttatc agaatcgtga tgtcaaagga atttttattt acgaggctat
      781 ctggattgta ggcatcgcct tgctgctggc atttttggtt gcacaagggg tgggaagttt
      841 ggcgaatgcg attgtaagtc acttttaccc atccatcact aaagtttttg aattaaatct
      901 tttatctgtt ttaggaactc tagttttcgc tctattactt ggttatgtct cagcctactt
      961 cccggcgcgt aagattagta aaatggatcc tgtagaaccg ttacgctatg aatagtaagg
     1021 gattccttga tttacagtct taaaaagaaa ctaacataga aaaacaatgg acatacttgc
     1081 aattcaactc aaatagttgt ataataggtt tgttgaaagg tgatttgtag tgattcaagt
     1141 tactcttttc acaataaaaa tttcatgatt ttcataagga ggaaatcact aatggtagtt
     1201 aaagttggta ttaacggttt cggacgtatc ggtcgtcttg ctttccgtcg tatccaaaac
     1261 gtagaaggtg ttgaagttac acgcatcaac gaccttacag atccagttat gcttgcacac
     1321 ttgttgaaat acgacacaac tcaaggtcgt ttcgacggta ctgttgaagt taaagaaggt
     1381 ggatttgaag ttaacggtaa attcatcaaa gtttctgctg aacgtgatcc agaacaaatc
     1441 gactgggcta ctgacggtgt agaaatcgtt cttgaagcta ctggtttctt tgctaagaaa
     1501 gaagcagctg aaaaacacct taaaggtgga gctaaaaaag ttgttatcac tgctcctggt
     1561 ggaaacgacg ttaaaacagt tgtattcaat actaaccacg acgttcttga cggtactgaa
     1621 acagttatct caggtgcttc atgtactaca aactgcttgg ctccaatggc taaagctctt
     1681 caagacaact ttggtgttgt tgaaggattg atgactacta tccacgctta cactggtgac
     1741 caaatgatcc ttgacggacc acaccgtggt ggtgaccttc gccgtgctcg cgctggtgct
     1801 gcaaacatcg ttcctaactc aactggtgct gcaaaagcta tcggtcttgt aatcccagaa
     1861 ttgaatggta aacttgatgg atctgcacaa cgcgttccaa ctccaactgg atcagttact
     1921 gaattggtag cagttcttga aaagaacgtt actgttgatg aagtgaacgc agctatgaaa
     1981 gcagcttcaa acgaatcata cggttacaca gaagatccaa tcgtatcttc agatatcgta
     2041 ggtatgtctt acggttcatt gtttgacgca actcaaacta aagttcttga cgttgacggt
     2101 aaacaattgg ttaaagttgt atcatggtac gacaacgaaa tgtcatacac tgcacaactt
     2161 gttcgtactc ttgaatactt cgcaaaaatt gctaaataat tcttgagttg atagaaagca
     2221 aggctttgtg gtcttgcttt ttatatggaa aaatggatga cacgatcatc cattcttttt
     2281 taattctttt tcaaatgtat ttgaaagggt agtgaaagtt agcttctcta aagtaagtgg
     2341 gtgggtaaag gaaagtcgga aggcatgaag cataagccga cttgtctttg atttactatt
     2401 atagagaggg tcgcccagga taggaagatt atgatgcgaa aggtgcacac gaatctgatg
     2461 ggttcgccct gtctttagct tgcaacgagc caaggaagtc ttgtttgaga attgctttaa
     2521 tctgcttaca tacgtttcag catattgccc attttttgca tcaactattc tttttctacg
     2581 atcatggcga tcacgtccaa ttttgtctct gaaaacaagt tcttttctgt tgatatttcc
     2641 atcaactaga gcccaatatt ctctagaaat ctcttttttc tccaataagc gattgagaat
     2701 gggcaggata aaaggatttt tggcaaagag aactaagcca ctggtttcca tgtccagacg
     2761 atgaacgaca tagcaggttt ggccaacata ggtactgaca tggttaagaa gggcaatttc
     2821 gtttggttga ttaccatgcg ttttcatccc ctctggtttg tttacaataa tcaagtattg
     2881 atcttgataa acttcctgca ctaagtctgg gttgccccaa gggatcgtct tttggggata
     2941 atcttcctcg tcaaaagtca actggcaaac atctccagga tttacgattt cgttccagtg
     3001 gacttcttct tgatttatca aaatatgttt cttgattctc aaaaaatgac ggatttttct
     3061 agggatgagg agttgttcct caagtaattg ctttaccgtc atttgaggta gagaggcggg
     3121 taatgtaaat gtgaattgca tacagatatt gtaacaaaaa aagccctatt tggataggaa
     3181 atagctaaat tcttgtcttc ctatgatgaa gatgataaaa taaacgcatg aaattagata
     3241 aattatttga gaaatttctt tctcttttta aaaaagaaac aagtgaacta gaggactctg
     3301 attctactat cttacgtcgc tctcgtagtg atcgaaaaaa attagcccaa gtaggtccga
     3361 ttcgaaaatt ctggcgtcgt tatcatctaa caaagattat ccttatacta ggtttgagtg
     3421 caggcttgct agttggaatc tatttgtttg ctgtagccaa gtcgaccaat gtcaatgatt
     3481 tgcaaaatgc cttgaaaact cggactctta tttttgaccg tgaagaaaaa gaggctggtg
     3541 ccttgtctgg tcaaaaggga acctatgttg agctgactga tatcagtaaa aacttgcaga
     3601 atgctgttat tgcgacagaa gaccgttctt tctataaaaa tgacgggatt aactatggcc
     3661 gtttcttctt ggctattgtc actgctggac gttcaggtgg tggctctacc attacccaac
     3721 agctggctaa aaacgcctat ttatcacagg atcaaactgt tgagagaaaa gcgaaagaat
     3781 ttttccttgc cttagaatta agcaaaaaat atagtaagga gcaaattcta accatgtacc
     3841 ttaacaacgc ttattttgga aatggtgtgt ggggtgtaga agatgcgagt aagaaatact
     3901 ttggagtttc tgcatcagaa gtgagtctgg atcaagctgc gactctggca gggatgctca
     3961 aggggccgga actgtataat cccttgaatt ccgtagaaga ttctactaat cggcgcgata
     4021 ctgtcttgca gaatatggtt gcagcaggat atattgataa aaaccaagaa accgaagctg
     4081 ctgaagttga tatggcttcg caattgcacg ataagtatga aggaaaaatc tcagattacc
     4141 gttacccctc ttattttgat gcggtggtta atgaagctgt ttccaagtat aatctaacag
     4201 aggaagagat tgtcaataat ggctaccgca tttacacaga gctggaccaa aactaccaag
     4261 caaatatgca gattgtttat gaaaacacat cgctatttcc gagggcagag gatggaacgt
     4321 ttgctcaatc aggaagtgta gctctcgaac cgaaaacagg gggagttcgt ggagttgtcg
     4381 gtcaagttgc tgacaatgat aaaactggat tccggaattt caactatgca acccaatcaa
     4441 agcgtagtcc tggttctaca attaagcctt tagttgttta tacgccagca gttgaagcag
     4501 gctgggcttt gaataagcag ttggataacc ataccatgca gtatgacagc tataaggttg
     4561 ataactatgc agggatcaaa acgagtcgag aagttcctat gtatcaagcc ttggcagaat
     4621 cgcttaatct acctgctgtt gccgctgtta atgatttggg tgtcgacaag gcttttgagg
     4681 caggcgaaaa attcggactc aacatggaaa aggtcgaccg tgttcttggt gtcgccttgg
     4741 gaagcggtgt tgaaaccaac cctcttcaaa tggctcaagc atacgctgcc tttgcaaatg
     4801 aaggtttaat gcctgaagct cattttatta gtagaattga aaatgctagt ggacaagtta
     4861 ttgcgagtca taaaaattca caaaaacggg tgattgataa gtctgtagct gacaagatga
     4921 ccagtatgat gttggggact ttcaccaacg gtaccggtat tagttcatcg cctgcagact
     4981 atattatggc agggaaaact ggaacaactg aagcagtttt caatccggag tacacaagtg
     5041 accagtgggt aattgcttat actccggatg tagtgattag ccactggctt ggctttccta
     5101 ccactgatga aaatcactat ctagctggct ctacttcaaa cggtgcagct catgtcttta
     5161 gaaacattgc caataccatt ttaccttata cgccaggaag tacctttacg gttgaaaatg
     5221 cttataagca aaatggaatt gcaccagcca atacaaaaag acaagtacaa accaatgata
     5281 atagccagac agatgataat ttgtctgata ttcgagggcg tgcgcaaagt ctagtagatg
     5341 aggctagccg ggctatctca gatgcgaaga ttaaggaaaa ggctcaaaca atatgggatt
     5401 cgatagtcaa tctatttcgc taagatgctt gtcaaagcct agctttcttg ttataatgga
     5461 taggatggag gcgttatggc actaaaaaaa gcaagcctag cttgtgcggt ttgtggttcg
     5521 agaaactatt caatcaagat cagcggaaac cccaagccta cacgactaga agtaaataaa
     5581 ttttgtaagc attgtggcaa gtacactaca cacagagaaa cgagatagga gagagcgatg
     5641 cgttttattg gagatatttt tagacttctt aaagacacaa cgtggccaac tcgcaaggaa
     5701 agctggagag attttcgttc tatcatggaa tacacagctt tctttgtagt aattatttac
     5761 atttttgacc agttgattgt ttcaggtttg attcgattta ttaacatttt ttagaagatt
     5821 agtggagtta attacactag aaatcttcta tattatgaaa ggaaatatca tggatagttt
     5881 tgataaaggg tggtttgttt tacaaactta ttctggttat gaaaataagg tgaaagaaaa
     5941 tctattacaa cgtgcacaaa cctacaatat gttggataat attctacgcg ttgaaattcc
     6001 aacacaaaca gtgcaagttg aaaaaaatgg aaagagaaaa gaagtagaag aaaatcgctt
     6061 tccaggttat gttcttgtag aaatggtcat gacagatgaa gcttggtttg ttgttcgaaa
     6121 cacaccaaat gttacaggat ttgtcggatc acacgggaac agatcaaaac caactccatt
     6181 attggaacaa gaaattcgtg acattttggt atctatggga caaactgttc aagaatttga
     6241 tttcgatgtt gagattggtc aaactgtacg tattattgat ggtgcttttg cagactacac
     6301 tggtaagatt acagaaattg ataataacaa agtgaaaatg attatctcta tgtttggtaa
     6361 tgacacagtt gcagaagtaa acctaaacca aatcgcagaa ttataaccct gagagaggct
     6421 ggagcctctc ttttttgtgc agtttaggag ctaaagggaa cagaatggag aaaatgtaac
     6481 aaatgtgttt tctaatctgt tagactgtat ctagaaaggg gaaaattatg attaaagaat
     6541 tgtatgaaga agtccaaggg actgt
//
LOCUS       DAVBBS010000086         6447 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVBBS010000086 DAVBBS010000000
VERSION     DAVBBS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.086"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            222..422
                     /locus_tag="VQU32_001993"
     CDS             222..422
                     /locus_tag="VQU32_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473388.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            447..863
                     /locus_tag="VQU32_001994"
     CDS             447..863
                     /locus_tag="VQU32_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473389.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1094..1801
                     /gene="ccdA2"
                     /locus_tag="VQU32_001995"
     CDS             1094..1801
                     /gene="ccdA2"
                     /locus_tag="VQU32_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET2473390.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1812..2369
                     /gene="sdbB"
                     /locus_tag="VQU32_001996"
     CDS             1812..2369
                     /gene="sdbB"
                     /locus_tag="VQU32_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HET2473391.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2490..3881
                     /locus_tag="VQU32_001997"
     CDS             2490..3881
                     /locus_tag="VQU32_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET2473392.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPSWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4024..4941
                     /gene="adcAII"
                     /locus_tag="VQU32_001998"
     CDS             4024..4941
                     /gene="adcAII"
                     /locus_tag="VQU32_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HET2473393.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            4949..>6447
                     /locus_tag="VQU32_001999"
     CDS             4949..>6447
                     /locus_tag="VQU32_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2473394.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGNHFHYIPKSDL
                     SASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLVFDPAQITYRTVNGVAVPHGDHYHFIPYSQLSPLEEKLA
                     RMIAVKGQNGAVLPGMHYLKPAPKPQVQPSTEKKQTDFAVEQVVRKVGEGYVVEIAGV
                     SHYVFAKDLAKDKIDAIENLLSKKTQETHALVAKKENVAPRDQEFYDKAYNLLTQAHK
                     VLSENKGRTSDFQALDKLAERLNNESSNKVKLVDDLLAFLAPITHPERLG"
BASE COUNT         1980 a         1172 c         1354 g         1941 t
ORIGIN      
        1 atggtttcag aaatttctct taccaacatt aaccacacca tcggttatta tagtaaaatg
       61 gaataagaac aggacaaatc gatcaggaca gtaaaatcga attctaacaa tgttttagaa
      121 gtctatatgt actattctag tttcaatata ccataactgc tcctagtcaa cctgcaagtc
      181 ctgtcccagc tccaagctca gctccgactc aagatcctag agtggtagca agcgcttcgg
      241 ctagctcaac gagcacacaa gctcaagagc aagttgacaa gtctgaactt cgtgccttga
      301 gtcaagagtt agaccaacgc ttgaaagctt tggcaacagt atctgatcca aaaatcgatg
      361 caaccaaggc tgtcctccta gatgctcaaa aagctccgga agatagtgcc ttgacagagt
      421 aaggtctgca tactgcagta gagtctgtga aggctgcctt ggactctctc aaagatgtga
      481 aagcaaatgc tagcgacagc aagcctgcac aggacaagaa ggatgcaaaa caaggaacgg
      541 aagatagtaa ggattcagat aagatgactg aaacaaactc agttccggca ggagtgattg
      601 tggtcagtct acttgccctc ctaggcgtga ttgccttctg gctgattcgc cgtaagaaag
      661 agtcagaaat ccagcaatta agcacggaat tgatcaaggt tctaggacag ctagatgcag
      721 aaaaagcgga taaaaaagtc cttgccaaag cccaaaacct tctccaagaa acccttgatt
      781 tcgtgaaaga agaaaatggc tcagcagaga cagaaactaa actagtagag gagcttaaag
      841 caatccttga caaactcaag taaaacatga tttatgaaat gtcagaccta aaagacattt
      901 tcgttcacaa aagcagtgac gagtaatcct tgtcgttgct ttttggctat tttgggggat
      961 aagatccgcc ttgactagca aaagaaaaga ttggtccaat agtgcccgat acgattttgc
     1021 gtagcagaaa gcttacaaat aaaattgaat atagtaaaat aggattagaa ttattaagaa
     1081 agttggttct ttattggaaa cgatagtatt tttaatctct gtttttctag caggtgtttt
     1141 atcctttttt tctccttgta tttttcctct tctgccagtc tatgctggga ttttattgga
     1201 tgatcaagaa agtgcaaaaa gcttttcttt gtttgggaga aaggttctct ggtcaggctt
     1261 gattcgaaca ctttgcttta tcgctggtat ctctctcatt ttctttattc taggctttgg
     1321 tgctggttac tttggtcata ttctctatgc aaattggttt cgatatgtca tgggagctat
     1381 tattatcatt ttgggtcttc accagatgga aatttttcat ttgaagaaat tagaagttca
     1441 aaaaagtttt acctttaaaa aatcagattc taatcgttat tggtcagctt ttttacttgg
     1501 tattaccttt agctttggtt ggacaccttg tattggtcca gttttaagtt ctgttttagc
     1561 acttgcggct tctggaggca atggtgcttg gcaaggagcg atttatactc tcatttacac
     1621 tctgggcatg gcccttcctt tcttggtctt ggcactagct tcaggtctag tcatgccata
     1681 ttttagtaaa atcaagcgtc atatgatgct actaaagaaa attggtggtt tcctcattgt
     1741 tttaatggga attttgttac tattaggaca agtaaatgtt ctagctggaa tttttgaata
     1801 aaggaggaca aatgaaaaaa gtaatgtttg ctggcttaag tctcttgtca ttagttgtat
     1861 tgatggcctg tggtgaggaa gaaactaaaa agactcaagc agcacaacag ccaaaacaac
     1921 aaacgactgt acaacaaatt gctgttggaa aagatgctcc agacttcaca ttgcaatcca
     1981 tggatggcaa agaagttaag ttatctgatt ttaagggtaa aaaggtttac ttgaagtttt
     2041 gggcttcatg gtgtggtcca tgcaagaaaa gtatgccaga gttgatggaa ctagcggcga
     2101 aaccagatcg tgatttcgaa attcttactg tcattgcacc aggaattcaa ggtgaaaaaa
     2161 ctgttgagca attcccacaa tggttccagg aacaaggata taaggatatc ccagttcttt
     2221 atgataccaa agcaaccacc ttccaagctt atcaaattcg aagcattcct acagaatatt
     2281 taattgatag ccaaggaaag attggaaaga ttcaatttgg tgctatcagt aatgcggatg
     2341 cagaagcagc atttaaagaa atgaactaga atcgataaaa tctgattaca agatgtagtc
     2401 agattttttt agaaaaacat tttataaata ttcacatatc tcctatattt atggtaaaat
     2461 agaattatca gtttattttg gagtcaaaga tgaatatatt tagaacaaag aatgttagtt
     2521 tagataaaac agagatgcat aggcatttga agttatggga tttgattttg ctgggtatcg
     2581 gagccatggt agggacaggc gtctttacaa tcacaggtac tgcagctgca acacttgctg
     2641 gcccagccct agtgatttca atcgttattt ctgccttgtg tgtgggatta tcagccctct
     2701 tttttgcaga atttgcctcg cgagtacccg ctacaggagg tgcctatagt tacctctatg
     2761 ctatcttagg agaattccct tcctggttgg ctggttggtt aaccatgatg gagttcatga
     2821 cagccatatc aggcgtagct tcgggttggg cagcttattt taaaggcttg ctctctcaat
     2881 acgggatagc ctttcctcag gctttaaatg gtacctttaa tcctcaagca gggacatttg
     2941 ttgatttatt gcctattctt gtcttggtct tggtgacttc gcttgtttta ctaaatgcca
     3001 aggcagcctt acgctttaat tcgattctag ttattttgaa attttccgct ttagctctct
     3061 ttgttttagt aggaatttgg aatattaagt ttgataattg gagcaatttt gctccttacg
     3121 gttttggaca aatctatgga gctagcactg gtattatggc tggtgcgtcc ttgatgttct
     3181 tcggtttttt gggatttgaa tccatctcta tggctgtaga tgaagtcaag actcctcaaa
     3241 aaaatattcc tagagggatt gtcttatcgc tttctatcgt aaccattctc tatgccttgg
     3301 tgacccttgt tttgactggt gtggttcact atagtcattt aaatgtcgat gatgccgttg
     3361 cctttgccct tcgtagtgtt gggattagtt gggcagccaa ctatgtgtca ttagtggcta
     3421 tcttgacctt gattacagtt tgtatttcga tgacctatgc cctatcgcgt atgatttaca
     3481 gtttagcacg tgacggctta gtgcctgctg cctttaagga actgacgaag actagcaaga
     3541 taccaaagaa tgctactatc ttgacaggtc tagcttcagc agtagcagca ggaatgttcc
     3601 cactagccag tattgcagcc tttttaaata tttgtacctt agcctacttg atcatgctgg
     3661 cttatggtct gattcgctta cggaaagaaa aaggaatgcc caaagctggg gaatttaaaa
     3721 caccattggt acctctgtta ccaattttat cgatcatcat ttgtttatcc ttcatgttac
     3781 agtataatat gaacacctgg attgctttcc tagttgcgtt gttagtagga agtattattt
     3841 acttcactta tggctataag cattctacca tagaagaata aagtgaaagt ctattgagtt
     3901 gttgacactc agtagatttt tatatgtgaa agaaatttca aattttttct aaaaatatct
     3961 tgacagatta aattttcagg agtagaatat ttactagtta attaaatggt taaggagttg
     4021 ttcatgaaga aacaaaattt atttttagtc ctgttaagtg tctttctttt gtgtttgggg
     4081 gcttgtggtc aaaaggaaag tcagacagga aaggggatga aaattgtgac cagtttttat
     4141 cctatctacg ctatggttaa ggaagtatct ggtgacttga atgatgttcg gatgattcag
     4201 tcaagtagtg ggattcactc ctttgaacct tcggcaaatg atatcgcagc catctatgat
     4261 gcagatgtct ttgtttacca ttctcataca ctcgaatctt gggcaggaag tctggatcca
     4321 aatctaaaaa aatccaaagt gaaggtctta gaggcttctg agggaatgac cttggaacgt
     4381 gtccctggac tagaggatgt ggaagcaggg gatggagttg atgaaaaaac gctctatgac
     4441 cctcacacat ggctagatcc tgaaaaagct ggagaagaag cccaaattat cgctgataaa
     4501 ctttcagagg tggatagtga gcataaagag acttatcaaa aaaatgcgca agcctttatc
     4561 aaaaaagctc aggaattgac taagaaattc caaccaaaat ttgaaaaagc gactcagaaa
     4621 acatttgtaa cacaacatac agccttttct tatctagcga agagatttgg gcttaatcaa
     4681 cttggtattg caggtatctc tcctgaacaa gaaccaagtc cacgacaact aacagaaatt
     4741 caggaatttg ttaagaccta taaggttaaa acgattttta cagaaagtaa cgcttcttca
     4801 aaagtagctg aaactcttgt caaatcaaca ggtgtgggtc ttaaaactct gaatccttta
     4861 gagtcagacc cacaaaatga caagacctat ttagaaaatc ttgaagaaaa tatgagtatt
     4921 ctagcagaag aattaaagtg aggaaagaat gaaaatcaat aaaaaatatc tagcaggttc
     4981 agtggcagtc cttgccctaa gtgtttgttc ctatgaactt ggtcgtcacc aagctggtca
     5041 ggttaagaaa gagtctaatc gagtttctta tatagatggt gatcaggctg gtcaaaaggc
     5101 agaaaatttg acaccagatg aagtcagtaa gagagagggg atcaacgccg aacaaattgt
     5161 tatcaagatt acggatcaag gttatgtgac ctctcatgga gaccattatc attactataa
     5221 tggcaaggtt ccttatgatg ccatcatcag tgaagaactt ctcatgaaag atccgaatta
     5281 tcagttgaag gattcagaca ttgtcaatga aatcaagggt ggctatgtga ttaaggtaga
     5341 cggaaaatac tatgtttacc ttaaagatgc agcccatgcg gacaatattc ggacaaaaga
     5401 agagattaaa cgtcagaagc aggaacgcag tcataatcac aactcaagag cagataatgc
     5461 tgttgctgca gccagagccc aaggacgtta tacaacggat gatgggtata tcttcaatgc
     5521 atctgatatc attgaggaca cgggtgatgc ttatattgtc cctcacggca atcacttcca
     5581 ctatattcct aagagtgatt tgtctgccag tgaattagct gctgcccaag cctattggaa
     5641 tgggaagcag ggatctcgtc cttcttcaag ttctagtcat aatgcaaatc cagctcaacc
     5701 aagattgtca gagaaccaca atctgactgt cactccaact tatcatcaaa atcaagggga
     5761 aaacatttca agccttttac gtgaattgta tgctaaaccc ttatcagaac gccatgtaga
     5821 atctgatggc ctagtatttg atccggcaca aataacatat cgtacagtca atggtgttgc
     5881 tgtaccgcac ggagaccatt atcactttat tccttattca caactgtcac ctttggaaga
     5941 aaaattggct cgtatgattg ctgttaaggg acaaaatggt gcagtcttgc caggaatgca
     6001 ttatttgaaa ccagcaccaa aaccgcaagt tcaaccatca actgagaaaa aacaaactga
     6061 ttttgcagta gaacaggttg ttagaaaagt tggagaaggc tatgttgtgg aaattgctgg
     6121 tgtcagtcac tatgtttttg ctaaagactt ggctaaggat aagattgatg ctattgaaaa
     6181 tctcttgtct aagaaaactc aagagacaca tgctctagtt gctaaaaaag aaaatgtcgc
     6241 tcctcgtgac caagaatttt atgataaagc ttacaatctg ttaacccagg ctcataaagt
     6301 cttgtctgaa aataaaggtc gtacttctga tttccaagcc ttagacaaat tagcagaacg
     6361 cttgaataat gaatcttcta ataaagtaaa attggtagat gatttattgg cattcctagc
     6421 accaattacc cacccagaac gccttgg
//
LOCUS       DAVBBS010000087         5104 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVBBS010000087 DAVBBS010000000
VERSION     DAVBBS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.087"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(189..266)
                     /locus_tag="VQU32_002000"
                     /pseudo
     CDS             complement(189..266)
                     /locus_tag="VQU32_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            424..642
                     /locus_tag="VQU32_002001"
     CDS             424..642
                     /locus_tag="VQU32_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HET2473395.1"
                     /translation="MKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVS
                     AIDSSEAMLLPTINVLELLDKSQPFEEE"
     gene            1687..2679
                     /locus_tag="VQU32_002002"
     CDS             1687..2679
                     /locus_tag="VQU32_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2473396.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYDGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            2681..3499
                     /locus_tag="VQU32_002003"
     CDS             2681..3499
                     /locus_tag="VQU32_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET2473397.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVIYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            3501..4286
                     /locus_tag="VQU32_002004"
     CDS             3501..4286
                     /locus_tag="VQU32_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2473398.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            4325..4603
                     /locus_tag="VQU32_002005"
     CDS             4325..4603
                     /locus_tag="VQU32_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HET2473399.1"
                     /translation="MFVIEEVKDENQKKAVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTRFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            4588..4785
                     /locus_tag="VQU32_002006"
     CDS             4588..4785
                     /locus_tag="VQU32_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2473400.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
BASE COUNT         1459 a          853 c         1074 g         1718 t
ORIGIN      
        1 agttagttga accgccgtat gccgaacggc acgtacggtg gtgtgagagg ggctagagat
       61 tatcccctac tcgatttcga aatctagtgg aatgaatctg gaatagtcca tcgagctttc
      121 taatactctt cgaaaatctc ttcaaaccac gtcaacgtcg ccttgccgtg cgtatggtta
      181 ctgacttcgt cagttctatc cacaacctca aaacagtgtt ttgagcaacc tgaggctagt
      241 ttcctagttt gctctttggt tttcatttag tataacagat tgttagaagt tggtttaaat
      301 ttcctaatca gtttgttcac atttaccttc gatatattat atcccatagt taaggttggt
      361 catacagatg attatagtca tggagccgta aaacttagtg tttctttagt tgacaaagat
      421 gccatgaaaa aaatatctgt aactgtaata ggatattttg aaataaatat agatgaaaat
      481 atcaccgata ttctatacgt aaatggtact gctattcttt atcattattt acgttctatt
      541 gtttcaatag tttcggcaat tgatagcagt gaagcaatgt tgctacctac cattaatgtt
      601 ttagagttac tagataaatc tcaacctttt gaagaagaat aatttattag ctcactaaat
      661 tgagggtaag gaaaagtaaa atcagtaaga aaaatgtctt gcattataca gcaacctttt
      721 gggaatgagt agatggattg aataaaattt tattaagagt ggatgattta tctgtagatt
      781 attattggac agttagtctt gaagtagtct aagaattagg ttataatcag tagaatcctt
      841 gctaataatg aggaggttag tttatgtata gtagactgaa tctaaaatag tacgaaacaa
      901 ttgctaaaac atttatagaa attaatttta ctttcccaat cgatttgttc tcatcttatt
      961 tcaatccgct atatattatg gtatcgaatc ttcatcagaa tgataaaatt aatcaattga
     1021 tatctgatta caaacagaat atgaaagctt tttatatcac tattgaaaaa tttatacgag
     1081 atgatgaaag ccttaagtgt tattttataa aggttatttc aagtcgttcc aaggtaacaa
     1141 gtctagatca gattgaagct gataaaacga tacaaagaaa atattcaagt gagctaaaaa
     1201 aatttattgg attttataat gagattattt gtgaggaaaa tagtttccta catgtacgaa
     1261 agaggtggtc gagttggttt aggtagtcga tgcgtgagtt gataattctc agggtatgga
     1321 cttctttttc atgaatgagg taaaagagca ggtattgttt agagacaatc attctgagca
     1381 tattttctgg atagagggag tatccgattt tatgatcaaa gttaataccg ccctctggtg
     1441 agaagatgag taggttggta atttaaacta ttaaacagaa tttttgatta aaagtattat
     1501 tttatgagag aaatcctaat ttcacaatcc ataggcaaac tcttgcattt cgttttttat
     1561 tggactataa taggttggta taaagccttt tgtagtaata aaatgtagaa ggtgtagaaa
     1621 gcaaggattt agaatatttg tagttaaaaa cacaatgttg ctattcctta cgatagggag
     1681 atagatatgg caatgataga agtggaacat cttcagaaaa attttgtgaa gactgttaag
     1741 gaaccgggct tgaagggggc tttgcgctcc tttattcatc ctgaaaagca gacctttgaa
     1801 gcggtcaagg atttgacctt tgaggttcca aaagggcaga ttttaggatt tatcggggca
     1861 aatggtgctg ggaagtcgac aaccattaaa atgctgacag gaattttgaa accaacatct
     1921 ggtttttgtc ggattaacgg caagattccc caggacaatc ggcaagatta tgtcaaagat
     1981 attggcgtag tctttggaca acgcacccag ctatggtggg atttggctct gcaagagacc
     2041 tacactgtct taaaagagat ttatgatgtg ccagactcgc tctttcataa gcgtatggac
     2101 tttttgaatg aagtcttgga tttgaaggac tttatcaagg atcccgtgcg gactctttca
     2161 ctgggacaac ggatgcgggc ggatattgcg gcctccttgc tccacaatcc caaggttctt
     2221 tttttagatg agccgaccat tggtttggac gtttcggtta aggataatat tcgtcgggca
     2281 attactcaga tcaatcaaga ggaagaaact accattcttt tgaccactca cgatttgagt
     2341 gatattgagc aactttgtga tcggattttc atgattgaca aggggcaaga gatttttgat
     2401 ggaacggtga gccaactcaa ggagaccttt ggtaagatga agactctctc ttttgaactg
     2461 ctaccaggtc aaagtcatct cgtctctcac tatgacggtc tgtctgatat gaccattgat
     2521 agacaaggaa acagcctcaa cattgaattt gatagttctc gctaccagtc agctgacatt
     2581 atcaagcaaa ccctgtctga ttttgaaatc cgcgatttga agatggtgga tacggatatt
     2641 gaggatatta tccgtcgctt ctaccgaaag gagctctagg atgatcaaat tgtggagacg
     2701 ttataaaccc tttatcaatg caggggttca ggagttgatt acttaccgag tcaactttat
     2761 tctctatcgg attggcgatg tcatgggggc ttttgtggcc ttttatctct ggaaggctgt
     2821 ctttgattct tcgcaagagt ctttgattca gggcttcagt atggcggata tcaccctcta
     2881 catcatcatg agttttgtga ccaatcttct gactagatcc gattcgtcct ttatgattgg
     2941 ggaggaggtc aaggatggct ccattatcat gcgtttgttg cgaccagtgc attttgcggc
     3001 ctcctatctt ttcaccgagc ttggttccaa gtggttgatt tttatcagcg ttggccttcc
     3061 atttttaagt gtcattgtct tgatgaaaat catatcgggt caaggtattg tagaggtgct
     3121 aggattaact gtcatttatc tttttagctt aacgctcgcc tatctgatta actttttctt
     3181 taatatttgc tttggatttt cagcctttgt gtttaaaaat ctttggggtt ccaacctact
     3241 taagacttcc atagtggctt ttatgtcggg gagtttgatt cccttggcat tctttccaaa
     3301 ggttgtttca gatattctct cctttttgcc tttttcatcc ttgatttata ctccagttat
     3361 gatcattgtt ggaaaatacg atgccagtca gattcttcag gcactccttt tgcagttctt
     3421 ctggctctta gtgatggtgg gattgtctca gttgatttgg aaacgggtcc agtcctttat
     3481 caccattcaa ggaggttagt atgaaaaaat atcaacgaat gcatctgatt tttatcagac
     3541 aatacatcaa acaaatcatg gaatataagg tggattttgt ggttggtgtc ttgggagtct
     3601 ttctggctca aggcttgaat ctcttgtttc tcaatgtcat ctttcaacat attccattcc
     3661 tagaaggctg gacctttcaa gagatagctt tcatttatgg attttccttg attcccaagg
     3721 gaatggacca tctctttttt gacaatctct gggcactagg gcaacgccta gtccgaaaag
     3781 gggagtttga caagtatctg actcgtccca tcaatcctct ctttcacatc ctagttgaaa
     3841 cctttcagat tgatgccttg ggtgaactct tagtcggtgg tattttattg ggaacaacag
     3901 tgaccagcat tgtttggact cttccgaaat tcctgctttt cctagtttgt attccttttg
     3961 cgaccttgat ttatacttct cttaaaatcg caacagccag tatcgccttt tggactaagc
     4021 agtcaggcgc catgatttac atcttctata tgttcaatga ctttgctaag tatccgattt
     4081 ctatttacaa ttctcttctt cgttggttga ttagctttat cgtgcctttc gcctttacag
     4141 cctactatcc agctagctat ttcttacagg aaaaggatgt gttctttaac gtaggaggtt
     4201 tgatgttgat ttctctggtt ttctttgtta tttcccttaa actttgggat aagggcttag
     4261 attcctacga aagtgcgggt tcgtaaaagc taaagtaaga ctaaaatcaa gaaagaaact
     4321 tatgatgttt gtaattgaag aagtcaagga tgaaaatcaa aaaaaggcag ttgtagctga
     4381 ggttttgaag gatttgccag aatggtttgg aatcccagaa agcacacaag cctatataga
     4441 aggaaccacg acactgcaag tttggaccgc ctatcaggag agtgatttga ctagatttgt
     4501 aagcttatcc tattcgagtg aagattgtgc agagattgat tgtctcggcg taaaaaagct
     4561 tatcaaggta gaaaaattgg gagccaattg cttgctactt tagagagtga agctcgtaaa
     4621 aaagttggtt atctgcaggt caaaacagtg gcagaaggtt ctaataaaga ttatgatcga
     4681 acaaatgact tttatcgagg tcttggcttt aaaaagttag agatttttcc tcaactatgg
     4741 aatccgcaaa atccttgtca gattttgatt aaaaagcttg aataatatta cttgacatct
     4801 attctcagag tgccatactg taagtgtaat cgccgattta gctcagttgg tagagcaagg
     4861 cactcgtaaa gcctaggtta taggtagata aacgactgag gagttgaaaa aatagatagg
     4921 tagaagataa ccgttaagcc ttattcttag cggttattta tattgtttaa tagcgctgat
     4981 atttcatcaa ttatgcctgt tttcgtgttt ctggtagttg ttcaagttta ttgctactat
     5041 ttttgatggt atgaatgtgc ttataatgta tcccggttaa cgaaagtttt ggacttatac
     5101 tctt
//
LOCUS       DAVBBS010000088         4552 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVBBS010000088 DAVBBS010000000
VERSION     DAVBBS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.088"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(112..549)
                     /locus_tag="VQU32_002007"
     CDS             complement(112..549)
                     /locus_tag="VQU32_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET2473401.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(634..3267)
                     /gene="polA"
                     /locus_tag="VQU32_002008"
     CDS             complement(634..3267)
                     /gene="polA"
                     /locus_tag="VQU32_002008"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET2473402.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            3520..3873
                     /locus_tag="VQU32_002009"
     CDS             3520..3873
                     /locus_tag="VQU32_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET2473403.1"
                     /translation="MILRHLGISPTNDLVAKKIFSNPEITCQFIRDMLDLPAKNVTIL
                     EGSNIHVLPSMPYSAQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQV
                     NQNLEKIRQREGDTH"
     gene            4088..4204
                     /locus_tag="VQU32_002010"
     CDS             4088..4204
                     /locus_tag="VQU32_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET2473404.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            4197..4430
                     /locus_tag="VQU32_002011"
     CDS             4197..4430
                     /locus_tag="VQU32_002011"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473405.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
BASE COUNT         1385 a         1096 c          837 g         1234 t
ORIGIN      
        1 gtatctacaa cctcaaaaca gtgttttgag cagcccgcga ctagtttcct agtttgctct
       61 ttgattttca ttgagtataa cacaaaaggt agcccatcag ctaccttttt cttatgcttc
      121 ctcaatcaag cgagtatgtt ctctcttgat acagcgattc atcacgatat catcacatcc
      181 accatcacgc aaaatctctt tcgcttctaa actttcaagt cctagctgtg cccaaaaaat
      241 cttggcatca gctttgagaa aatcacgcgc cacatcgggc agaaattcac tgcgacgata
      301 aacattgaca atatctacag gaaaaggaat ttcagcgagg ctagcataag ccttttcacc
      361 caagatttcg ccacctgccg ccttgggatt gactgggatg attttatagc cccgagcctg
      421 catttccttt gtcactcgat tgctggttgt ttcttcacgg tcagacaaac ccaccacagc
      481 aagggtttta ctcgttgcga gatactgacg aatcacgcca tcacttggat tgataaattc
      541 ttgactcata gaaaacctcc tttttcatca gtatagcaca ttttgaaaag gtttgcagaa
      601 ttctactaca aaaaaggagg actagccccc tttttattta gcctcgtacc aggttgcccc
      661 ttcattctca tctgcgataa gaggaacact gagttgaatg gcttcttcca tggtttgttt
      721 caccaatttt ttcatctcta ccaattcaga tttaggcact tcaaggacga tttcatcgtg
      781 cacttgtaac agcatcttag tctgataacc acctgcaacc aaggctttat ccagctgaat
      841 catggcaatc ttgagaatat ctgctgccga accctggata ggtgagttga tagcagttcg
      901 ctccgcaaaa ccacgaatat tgaagttgcg cgaattgata tctggcaact cacggcgacg
      961 cttaaagagg gtctctacat agcccttatc acgcgcctcc cgcaccactt catccatgta
     1021 gtttttaata cctggaaaac gttcaaagta ggtatcaatg taggctttgg cttccttacg
     1081 actaattccc aaattattag acaagccaaa gtctgaaatc ccataaacca ctccaaagtt
     1141 aactgccttg gcattgcgac gatcatttgc agtcacatta tcaggacgct caatgccaaa
     1201 gacccgcatg gctgtcgaag tatggatatc tgccccctct tggaaggcct taatcaagtg
     1261 ctcatcctta gaaatatgcg ccaaaacgcg caattcaatc tgtgaatagt cagagctgag
     1321 tagcacacta tcctcccact ctggcacaaa agccttccga atcaagcgcc cttgttccaa
     1381 tcgggcagga atattttgca agtttggatc cacactagac aaacgcccgg tctgggtcaa
     1441 atcctgcata tagcgagtat gaatctttcc atcagccaaa atccagtcct gcaagccaat
     1501 tacataagta gattgaatct tagcaatttg acggtaatcc aggattttct taacaatcgg
     1561 agcaatagga gcgagacgct ccaacacatc cactgctgtc gaataacctg tcttggtttt
     1621 cttagtgtat tctagaggaa gtcccaattt ctcaaagaga agcacgccca actgcttagg
     1681 cgagttgaca ttaaactcct caccagccag ctcgtaaatc tcttgagtca gtttttcaat
     1741 gacaagctca ttttcagcct gcatctcaag caaggtctct ttcttgacca caatcccagc
     1801 aatttccatc ttggcaagaa caaaagccag aggttgctcc atatcataaa gaagctctaa
     1861 ttgcccattt tcgctgagtt tttcaagtaa aataggctct gtttctacca aaacagcaag
     1921 tttacaagct aagtgttcca agaatttctc acgttcagga atggcctttt taacaccctt
     1981 accgtagaaa gtttcatcat caaccaagta agtctgacca taaagactag cgatggtcgc
     2041 aatttcattg tcctccacag tcgaaaggag gtatttagcc aaacggatgt caaaagcagg
     2101 cgcctgcaaa tccacaccaa aacgttgcaa aagaacttta accttcttaa agtcataaac
     2161 tctcagagat gttttttcta agaaatcctt gaaaatcggg tcttgcaaca gctcaagctt
     2221 gtctgtggca tagagcttat ccccacaaga ccaggcaaat ccaaccaaat tatccgtatg
     2281 ataattctca ccaaaaagct caaagtggaa gatagactct tcactcagca tatcttgact
     2341 gatttggtca acaatagtaa aatccaaacc ctcagccaca tcagctgacg acacatttaa
     2401 agcctgcttg agctgtttga atcccatctc atcgtaaaat ttcccaagat tttcaacatc
     2461 tggaccacta tagaccaagt cctctaaacc aatcgcaatc ggtgccttgg tatcaatggt
     2521 cgctagtgtt ttagacaaaa aggcctgttc cttgtcattg atgagatttt ccttcatctt
     2581 agaagtcttc attccatcaa tattttcata aatcccctca agcgaaccat gctccagcaa
     2641 gagcttaata cccgtctttt caccgacttt ggtcacccca gggatattat ccgacttatc
     2701 acccatgagc gccttgagat cgataaactg agctggtgtg aggcccattt cttccatgag
     2761 gtaatctggc gtaaaggcct caaactcagc cacacctttc ttggaaattt caaccaccgt
     2821 atgctcatcc gtcagctgaa tcaaatcctt gtccccactg acaatggtaa tatcaaaacc
     2881 atcctgctct gctagcttat ccaacgtccc aatgatgtca tccgcctcat actgagccag
     2941 ctcatagtga cgaatcccca tatgatccag caactcacga atgaaaggaa attgctcacg
     3001 aaactcatca ggagtcttgg cccgaccacc cttatagtcc gcatacatct ctgtccggaa
     3061 ggtcgtcttt cccgcatcaa aagccaccaa aatatgactc ggctcaaccc gctccaacaa
     3121 atgactcaac atcaactgaa aaccataaat cgcattggta tgcaaaccag ccgcattctt
     3181 aaaacggtcc aactgctgat acagcgcaaa aaacgcccga aaagctacag aagacccatc
     3241 aatcaataat aattttttct tatccataca cccattataa aggaaagaat caaaaaatac
     3301 cattgggaag agctagagca agtatttttc aaactttttc cgaataaata gatagagcca
     3361 gagaatttag taaacctaga tttaaaaatg tgctataata tagtatattg aatctataat
     3421 agtacacctt gactgctaaa atatttctat aaattaattt gactttcctg atagagttgt
     3481 tcacatctta tttcaattca ctatagaagg aggaaaagga tgattctcag acatctggga
     3541 attagcccga ccaatgactt ggttgctaag aaaattttca gcaacccaga aatcacttgt
     3601 caatttatcc gcgatatgct ggacttgcca gccaaaaatg tgactatttt ggagggaagt
     3661 aacattcatg tcttgccttc catgccctac tcagcgcagg atttttatac cagtatagac
     3721 gtcttggcgg agttggataa cggtactcaa gtaattattg agattcaagt ccatcatcag
     3781 aattttttca tcaatcactt gtgggcttac ctgtgcagtc aggttaatca aaatcttgaa
     3841 aaaattcgtc agcgagaagg tgatactcac tagatctaca aacacatcgc tcctgtttac
     3901 gtcattgcta tcgtggatag taattatttc tcagatgacc tggcttttca tagctttagt
     3961 atgcgcgaag acacaacagg tgaggtattg gcgattacca acaatggaca ggaaaaccat
     4021 ctggttaaga tggcattctt ggaattaaaa aatacagaga aaccagcaaa gacaaggttc
     4081 gcaagccatg gttggagttt ttcggcaaca agccctttac ccagcaaccg caacgagcca
     4141 ttacccaagc aaatcaactg ctggactaca agagctggtc cgaggaggac aggaaaatgt
     4201 ttagtcaact acatatgcga gaagaacaag tcttgttagc acaggactat gccttggaaa
     4261 ctgctagggc tgaaggcctt gaacaaggac tagagcgtgg gaaagttgaa ggaagggcag
     4321 aaaggaaact ttttgccttc ctagacatag tacgccaagg tcttctgact tctgaggttg
     4381 ccagccagca attaggtatg tcagtatctg aatttgaggc actgttgtaa aatggctcca
     4441 taatatccat agtgggtaaa tcccctatgg atattatgga gcctattttt gtgtagaaaa
     4501 aaagtcccat atgacctata atgaaaagcg acaaaacaac tcattaaaaa ga
//
LOCUS       DAVBBS010000089         4479 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVBBS010000089 DAVBBS010000000
VERSION     DAVBBS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.089"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..1261
                     /locus_tag="VQU32_002012"
     rRNA            <1..1261
                     /locus_tag="VQU32_002012"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1313..1385
                     /locus_tag="VQU32_002013"
     tRNA            1313..1385
                     /locus_tag="VQU32_002013"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1346..1348,aa:Ala,seq:tgc)
     gene            1510..4411
                     /locus_tag="VQU32_002014"
     rRNA            1510..4411
                     /locus_tag="VQU32_002014"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1239 a          918 c         1333 g          989 t
ORIGIN      
        1 atacatagcc gacctgagag ggtgatcggc cacactggga ctgagacacg gcccagactc
       61 ctacgggagg cagcagtagg gaatcttcgg caatggacgg aagtctgacc gagcaacgcc
      121 gcgtgagtga agaaggtttt cggatcgtaa agctctgttg taagagaaga acgagtgtga
      181 gagtggaaag ttcacactgt gacggtatct taccagaaag ggacggctaa ctacgtgcca
      241 gcagccgcgg taatacgtag gtcccgagcg ttgtccggat ttattgggcg taaagcgagc
      301 gcaggcggtt agataagtct gaagttaaag gctgtggctt aaccatagta ggctttggaa
      361 actgtttaac ttgagtgcaa gaggggagag tggaattcca tgtgtagcgg tgaaatgcgt
      421 agatatatgg aggaacaccg gtggcgaaag cggctctctg gcttgtaact gacgctgagg
      481 ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgct gtaaacgatg
      541 agtgctaggt gttagaccct ttccggggtt tagtgccgta gctaacgcat taagcactcc
      601 gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc ccgcacaagc
      661 ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc ttgacatccc
      721 tctgaccgct ctagagatag agttttcctt cgggacagag gtgacaggtg gtgcatggtt
      781 gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca acccctattg
      841 ttagttgcca tcatttagtt gggcactcta gcgagactgc cggtaataaa ccggaggaag
      901 gtggggatga cgtcaaatca tcatgcccct tatgacctgg gctacacacg tgctacaatg
      961 gctggtacaa cgagtcgcaa gccggtgacg gcaagctaat ctcttaaagc cagtctcagt
     1021 tcggattgta ggctgcaact cgcctacatg aagtcggaat cgctagtaat cgcggatcag
     1081 cacgccgcgg tgaatacgtt cccgggcctt gtacacaccg cccgtcacac cacgagagtt
     1141 tgtaacaccc gaagtcggtg aggtaaccgt aaggagccag ccgcctaagg tgggatagat
     1201 gattggggtg aagtcgtaac aaggtagccg tatcggaagg tgcggctgga tcacctcctt
     1261 tctaaggata aggaactgcg cattggtctt gtttagtctt gagaggtctt gtggggcctt
     1321 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc agcggttcga tcccgctagg
     1381 ctccattggt gagagatcac caagtaatgc acattgaaaa ttgaatatct atatcaaata
     1441 gtaacaagaa aataaaccga aaacgctgta gtattaataa gagtttatga ctgaaaggtc
     1501 agaaaataag gttaagttaa taagggcgca cggtggatgc cttggcacta ggagccgacg
     1561 aaggacgtga caaacgacga tatgccttgg gtagctgtaa gtaagcgatg atccagggat
     1621 ttccgaatgg gggaacccaa caggtagtac ctgttaccca catctgttaa ggatgtgagg
     1681 aggaagacgc agtgaactga aacatctaag tagctgcagg aagagaaagc aaaagcgatt
     1741 gccttagtag cggcgagcga aacggcagga gggcaaaccg aagagtttac tcttcggggt
     1801 tgtaggactg caatgtggac tcaaagatta tagaagaatg atttgggaag atcagccaaa
     1861 gagagtaata gcctcgtatt taaaatagtc tttgtaccta gcagtatcct gagtacggcg
     1921 ggacacgtga aatcccgtcg gaatctggga ggaccatctc ccaaccctaa atactcccta
     1981 gtgaccgata gtgaaccagt accgtgaggg aaaggtgaaa agcaccccgg gaggggagtg
     2041 aaatagaacc tgaaaccgtg tgcctacaac aagttcgagc ccgttaatgg gtgagagcgt
     2101 gccttttgta gaatgaaccg gcgagttacg ttatgatgcg aggttaagtt gaagagacgg
     2161 agccgtaggg aaaccgagtc tgaatagggc gccttagtat catgacgtag acccgaaacc
     2221 atgtgaccta cccatgagca ggttgaaggt gcggtaagac gcactggagg accgaaccag
     2281 ggcacgttga aaagtgcttg gatgacttgt gggtagcgga gaaattccaa acgaacttgg
     2341 agatagctgg ttctctccga aatagcttta gggctagcgt cgacattaga gattcttgga
     2401 ggtagagcac tgtttgggtg aggggtccat cccggattac caatctcaga taaactccga
     2461 atgccaatga attatggtcg gcagtcagac tgcgagtgct aagatccgta gtcgaaaggg
     2521 aaacagccca gaccaccagc taaggtccca aaataattgt taagtggaaa aggatgtggg
     2581 gttgcacaga caactaggat gttagcttag aagcagctat tcattcaaag agtgcgtaat
     2641 agctcactag tcgagtgacc ctgcgccgaa aatgtaccgg ggctaaaaca atttaccgaa
     2701 gctgtggata cctttatagg tatggtagga gagcgttcta tgtgtgatga aggtataccg
     2761 tgaggagtgc tggaacgcat agaagtgaga atgccggtat gagtagcgaa agacaggtga
     2821 gaatcctgtc caccgtaaga ctaaggtttc caggggaagg ctcgtccgcc ctgggttagt
     2881 cgggacctaa ggagagaccg aaaggtgtat ccgatggaca acaggttgat attcctgtac
     2941 tagagtatgt agtgatggag ggacgcagta ggctaactaa agcagacgat tggaagagtc
     3001 tgtctaagca gtgaggtgtg atatgagtca aatgcttata tctgtaacat tgagctgtga
     3061 tggggagcga agtttagtag cgaagttagt gacgtcacac tgccaagaaa agcttctagc
     3121 gtttaaacat actctacccg taccgcaaac cgacacaggt agtcgaggcg agtagcctca
     3181 ggtgagcgag agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa
     3241 ggggtgctga ctttaagtca gccgcagtga ataggcccaa gcaactgttt atcaaaaaca
     3301 cagctctctg ctaaatcgta agatgatgta tagggggtga cgcctgcccg gtgctggaag
     3361 gttaagagga gtgcttagcg taagcgaagg tatgaattga agccccagta aacggcggcc
     3421 gtaactataa cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acccgcacga
     3481 aaggcgtaat gatttgggca ctgtctcaac gagagactcg gtgaaatttt agtacctgtg
     3541 aagatgcagg ttacccgcga caggacggaa agaccccatg gagctttact gcagtttgat
     3601 attgagtgtc tgtaccacat gtacaggata ggtaggagtc taagagatcg ggacgccagt
     3661 ttcgaaggag acgctgttgg gatactaccc ttgtgttatg gccactctaa cccagatagg
     3721 tgatccctat cggagacagt gtctgacggg cagtttgact ggggcggtcg cctcctaaaa
     3781 ggtaacggag gcgcccaaag gttccctcag aatggttgga aatcattcgc agagtgtaaa
     3841 ggtataaggg agcttgactg cgagagctac aactcgagca gggacgaaag tcgggcttag
     3901 tgatccggtg gttccgtatg gaagggccat cgctcaacgg ataaaagcta ccctggggat
     3961 aacaggctta tctcccccaa gagttcacat cgacggggag gtttggcacc tcgatgtcgg
     4021 ctcgtcgcat cctggggctg tagtcggtcc caagggttgg gctgttcgcc cattaaagcg
     4081 gcacgcgagc tgggttcaga acgtcgtgag acagttcggt ccctatccgt cgcgggcgta
     4141 ggaaatttga gaggatctgc tcctagtacg agaggaccag agtggactta ccgctggtgt
     4201 accagttgtc ttgccaaagg catcgctggg tagctatgta gggaagggat aaacgctgaa
     4261 agcatctaag tgtgaaaccc acctcaagat gagatttccc atgattatat atcagtaaga
     4321 gccctgagag atgatcaggt agataggtta gaggtggaag tgtggcgaca catgtagcgg
     4381 actaatacta atagctcgag gacttatcca aagtaactga gaatatgaaa gcgaacggtt
     4441 ttcttgaatt gaatagatat tcgattttga gtaggtatt
//
LOCUS       DAVBBS010000090         4438 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVBBS010000090 DAVBBS010000000
VERSION     DAVBBS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.090"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..119
                     /locus_tag="VQU32_002015"
     CDS             <1..119
                     /locus_tag="VQU32_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2473406.1"
                     /translation="QQGFTDRLVGYSSSIALVFTTLIGMISLVQRRVLKEDD"
     gene            121..966
                     /locus_tag="VQU32_002016"
     CDS             121..966
                     /locus_tag="VQU32_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2473407.1"
                     /translation="MKRFKPTTILEYILLTLLAGLFLFPMVWMVVSAMKPEADVYKNL
                     TSWQAFLPSLNPADWFKPYQEVFERFSIFTYLGNSIFYAGTFAIGSIIVNALAGFAFA
                     KVNFSGKKILFGFLLALLIIPVETVLIPQFTIINSLGLVNNRLAVIIPGLASVFNIYL
                     FRNFFIAIPEEILESAKMDGASIVRIFFRIMLPMSKPAVATVGVLSFISSWNDYIWPL
                     MVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTISTIPLILVYVVAQKYIVQGLGGS
                     GTGIK"
     gene            976..2295
                     /locus_tag="VQU32_002017"
     CDS             976..2295
                     /locus_tag="VQU32_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET2473408.1"
                     /translation="MINKTFTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKISQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWREKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(2503..2575)
                     /locus_tag="VQU32_002018"
     tRNA            complement(2503..2575)
                     /locus_tag="VQU32_002018"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2540..2542),aa:Lys,seq:ctt)
     gene            complement(2839..4110)
                     /locus_tag="VQU32_002019"
     CDS             complement(2839..4110)
                     /locus_tag="VQU32_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET2473409.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWIKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
BASE COUNT         1294 a          809 c          976 g         1359 t
ORIGIN      
        1 atcaacaagg ttttactgat cgtcttgtag gatattctag ctcaatcgct ttagtattta
       61 caacgttaat tggtatgatt agcttagtac aacgtcgcgt tctgaaggag gatgactaag
      121 atgaaacgat ttaaaccaac aacaatctta gaatatattc tgttaacttt attggcagga
      181 ctcttccttt ttcctatggt ttggatggta gtttcagcta tgaagccaga agcggatgtt
      241 tataaaaatt tgactagttg gcaagccttc ttaccaagtt taaatcccgc tgattggttt
      301 aaaccttatc aggaagtatt tgagcgcttt agtattttta catatctagg gaacagcatt
      361 ttttatgctg gaacgtttgc tattggttct attattgtca atgcactggc cggtttcgct
      421 tttgcaaaag tcaatttttc aggtaagaaa attttatttg gtttcttact tgctttactt
      481 attattccag tggaaactgt gttaattcca caatttacca ttatcaattc cttaggtttg
      541 gtcaataatc gtcttgcagt tattattcca ggattggcga gtgtatttaa tatttactta
      601 tttaggaatt tctttattgc aattccagaa gagattcttg agtctgcaaa gatggacgga
      661 gcaagtatcg ttagaatttt ctttagaatt atgcttccaa tgtctaaacc tgcagttgct
      721 acagttggtg ttttatcatt tatttcaagc tggaatgatt atatctggcc attgatggtt
      781 ttgacagatt cttctaaatt ttcaatgcag gttgcaatta caactattaa tacaactcaa
      841 ccagtttata tcaatcaggt aatggctgta ttaactattt caacgattcc gttgattttg
      901 gtctatgtag tcgctcagaa atatattgtt caaggtctgg gtggttccgg aactggaata
      961 aaataggata tacttatgat aaataaaaca tttacagtag aaaaagccaa tcgttttata
     1021 gcagaaaata aacatctcgt taatactcaa tataagcctg aagaacattt ttcagctgag
     1081 attggttgga tcaatgatcc aaatggattt gtctattttc gtggagaata ccatctcttt
     1141 tatcaattct atccatatga tagtgtttgg gggcctatgc actggggaca tgctaaaagt
     1201 aaggacttgg tgacttggga gcacttgcca gtggcacttg ctcctgacca agattatgac
     1261 cgaaatggtt gtttctcagg ctctgccatt gtcaaggatg atcgcctctg gctcatgtac
     1321 actggacata tcgaagaaga aaccggtgtc cgccaagtgc aaaatatggc attttcagat
     1381 gacgggattc actttgaaaa gatttcccaa aatccagttg caactggatc agacttgcca
     1441 gatgagttga ttgctgctga tttccgtgat ccaaaactct ttgaaaaaga tggacgctat
     1501 tactccgtag tagctgccaa acacaaggat aatgtgggct gtatcgttct actagggtcc
     1561 gataacctag tagaatggca gttcgaatcc atctttttaa aaggaggaga acaccaaggc
     1621 tttatgtggg aatgcccaga ttacttcgag ttagatggga aagattgcct tattatgtca
     1681 cccatgcgtt atcagcgtga gggagactca tatcataaca tcaactcatc gcttttgttc
     1741 acgggtaagg tagattggag agaaaaacgt tttatcccag aatcagttca agaaattgat
     1801 catggccaag acttctatgc gcctcaaaca ttgttggacg atcaaaatcg tcgtatcctg
     1861 attgcttgga tgcagacatg ggggcgtacc cttccaaccc atgaccaaga acacaaatgg
     1921 gcatgtgcca tgactttacc tagaattcta agattggaag atggcaaact aagacaattc
     1981 cctgttaaaa aaggccaata tcaaatccaa atagataaag attgtcatta ccacttagga
     2041 aatgatacag attatcttga atttggttat gacagtaatg cgcagcaagt ttacattgat
     2101 cgtagccatc ttattcaaaa aattctaggt gaagaagaac aggacactag tcgacggtat
     2161 gtagatattg aagctaaaga attggaagtt gttctagata aaaattccat cgagattttt
     2221 gtcaatcaag gtgaagcaag cttgactgca acttattact taacggtgcc agctgagcta
     2281 tcacgaattg attaaaaatt aagttatttc tcctaaagaa aaagttctct ttctaaaata
     2341 gtggaaagag gactttttgt gttttgggta tataagctta gtttatggta tttgtaaaat
     2401 tggtgttgga ttatgattta agctagtttt ctaaagaatt tgaaaaaaat tttatttaag
     2461 caaaaaaacc ttggttccaa ggcttttcct gttgtattta gatgccccct acagggatcg
     2521 aacctgtgac ccacggatta agagtccgct gctctgccag ctgagctaag gaggcaaaga
     2581 aaaagctgta ttggtgccga aacttcacgg tttgtattga acccgcgcaa ttaagcaggt
     2641 gggcaactcg ctctaactga agctgtttcc gtgtgagacg gcctacatgc tgttagaaga
     2701 cttttgtttc cctaataata caaaaaatag tcggtcaaca cttaagtgtg aagtcgtaca
     2761 ccacagcgtt tctatactta tatgatacca ctttttcaaa aaaaatcaag aggaaagtgc
     2821 aattttttga aaaggatttc agatttttcg caaacgatcg atagcttcct ttctttgagc
     2881 caaagcccgt tcatatttac cagtatcttc tgcttggaaa tagtgatgat tacgaatttt
     2941 ttctggcaga tagtcttgct tgatccaatt tccaggatag ttgtgtggat agagatagtc
     3001 ttgggcattc cccagttcct tgcttccact gtagtgccca tcacgcaggt gtcgcggaat
     3061 aggcaagtgc cctgatgttt tgaggtcagc aagtgcctta tccatagcta cataggctga
     3121 gttggatttt ggagaaaggg ccaaatcaat cacgacattg gcaatgagaa tgcgggcttc
     3181 ggggaaacca atcctttgtg cagcatccag agcagtcacg gtatgaatct gggcctcagg
     3241 attggctaag ccaatatctt cataggcgat aacagtcaag cgacgagcga gactaggcag
     3301 atccccagcc tcaatcaagc gggcagcata gtggagactg gcatccacat ctgagccacg
     3361 gatagacttt tgcagggcag atagaacatc atagtgaccg tctccatcct tgtccatagt
     3421 gatgtagctt ctctgaagac tattttccat gatgtctaag gtgatgtggc gaatgccctc
     3481 gtcattctca ggggtagaga gaacagccaa gtccagtgag ttaaaggcag agcgaaggtc
     3541 tccgtttgta gaggttgcga tgaaatccag cgcatcctca tctagttcta ttggaaaatc
     3601 aaaaccacgt tcagggttac ttagagctat ctgcagggcc tctttgacgt cttggttaga
     3661 cagaggttcc aactcgaaaa tttgaacacg gctacgaatg gcaggagtga cagagaagaa
     3721 aggattttca gtcgttgctc caatcatgat gaccagtcca ctttccaaga gaggcaagag
     3781 gaagtcttgc ttggtcttat ctagtcgatg aatttcgtct agcaatagga cgagaccacc
     3841 agaaaattta gcttcttccg agatttcttg cagtcgcttt ttactatcaa ctgtcgcatt
     3901 gaaagttcga aaggcatact tggtcgttcc agcgatggca gaggcaatac tggttttgcc
     3961 gattccagga gggccatata gaatcatgga ggacaggcgg ttggcttcca ccatgcggcg
     4021 gatgattttt ccaggtccga ccagatgctc ctgaccgatg acctggtcga tggttttagg
     4081 gcgcatgcga agcgcgagat tgtctggcat agcagtcctt tctaacgtgg atttgagtag
     4141 aatgactcat ctactatatc ttactatgtc cattctagcc tattagttgt gatatttcaa
     4201 tggttttgta ataatcaggg agtgagatag aatctttttt actatttaat ttaatgagga
     4261 gaggtatcag catgagagat ttgattttcc ttaaagctca aaataggata gttgagccta
     4321 aagaagtgga agtgctgact tgagaaataa tattgctgaa tgtcattgta tgaactataa
     4381 tgaaaagaga ggtgataagc aatggaaatg atggaccaag tcaggaaatt ttgatttt
//
LOCUS       DAVBBS010000091         4423 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVBBS010000091 DAVBBS010000000
VERSION     DAVBBS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.091"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(56..1066)
                     /locus_tag="VQU32_002020"
     CDS             complement(56..1066)
                     /locus_tag="VQU32_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HET2473410.1"
                     /translation="MSFLSKNGAGILACLLISIVSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIDAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKELSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            1215..2384
                     /locus_tag="VQU32_002021"
     CDS             1215..2384
                     /locus_tag="VQU32_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2473411.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            2381..3151
                     /gene="recO"
                     /locus_tag="VQU32_002022"
     CDS             2381..3151
                     /gene="recO"
                     /locus_tag="VQU32_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET2473412.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            3148..4140
                     /gene="plsX"
                     /locus_tag="VQU32_002023"
     CDS             3148..4140
                     /gene="plsX"
                     /locus_tag="VQU32_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET2473413.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            4146..4379
                     /locus_tag="VQU32_002024"
     CDS             4146..4379
                     /locus_tag="VQU32_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET2473414.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQSVGDVVKIIQGK"
BASE COUNT         1286 a          842 c         1135 g         1160 t
ORIGIN      
        1 aacatatata cggcaaggcg acgttgacgt ggtttgaaga gattttcgaa gagtgttaga
       61 aaatgccgat aagggtctgc ataccaagag tggtgaggat gatggcaatc cagcagatgg
      121 ctccaagaag aatggatttt ccactggatt tgaccatagc gaccagattg gttttgagac
      181 cgatagcact catggccatg acaataagga atttagagag ttctttgaga ggggtaaaga
      241 aactactgga cacacctaga gaggtgagta gtgtagtgag gagagaggca aggataaagt
      301 aaaggataaa aagtgggaag acttttttca gttgtaggct ttgcttgttt tcttgttggc
      361 gactttgcca gtaggagaga aagagcgtga tagggataat agccaaagta cgtgtgagtt
      421 taacaatggt tgcagactcg agggtattgc tttggtaaag actgtcccaa gcgctggcgg
      481 cagccgttac agaggaagtg tcgttgaccg cagtccctgc aaagagggcg aagccttcat
      541 tggatagatg aagccaggtg ccgagggttg gaaagatgag cgcagccaag acattgaaga
      601 aaaagataac ggaaatggct tgggctactt ccttttcctt agcatcaata acgggcgctg
      661 tcgctgcaat ggcagaaccc ccacagatag aagaacctac tccaaccaag gtagccagtt
      721 ttgtatccag tgcaaagaaa cgctggaaga ggtaggcaat aatcagagct attgagatag
      781 tggacaggat gacagggagt gaagattggc caactgcgaa gacctgcgag atattgagac
      841 caaaaccaag caagacaacg gcatattgga gcaacttctt ggaactaaag gtcaaaccag
      901 catccagttg tttataggac gagagaaagg gatgtaggag catgcctatg aaaatggcaa
      961 aaacgggcgc gccaatcaca gggaagaatc ctcctaagta ccaagatacg atagaaatga
     1021 gaaggcaggc caagatacct gctccatttt ttgatagaaa tgacataaaa acctccaaat
     1081 aaaatctatt accattatag acctataaac aggaaaagta aaatagaaag tggaaagcta
     1141 ttctctaatg tatttttgcg gtcggggggc ttttgtagta taatagagat acgttttgaa
     1201 agtaggaggt atctatggac ttaactaagc gctttaataa acagttagat aaaattcaag
     1261 tttcgttgat tcgtcagttt gaccaggtta tttcggagat tcctggggtc ttgcgtttga
     1321 ccttggggga acctgatttt acaacgccag accatgtcaa ggaggcggcc aagcgagcga
     1381 ttgatcagaa ccaatcctac tatacaggga tgagtggtct gctgactcta cgtcaggcag
     1441 ccagtgactt tgttaaggaa aagtaccaac tggactatgc tcctgaaaat gaaattttgg
     1501 ttacaattgg ggcgacagag gctttatctg cgactttgac ggctattttg gaagagggag
     1561 acaaggtact tttgccagct cctgcttatc caggctatga accgattgtt aacttagttg
     1621 gggcagaaat tgttgagatt gatacgactg aaaatggttt tgtcttgact cctgagatgt
     1681 tggagaaggc cattttggag cagggtgata agctcaaggc ggttattctc aactatccag
     1741 ccaatccgac aggaattacc tacagtcgag agcagttaga ggccttggca gctgttttac
     1801 gcaagtacga aatttttgtt gtctgtgatg aggtttactc agaattgacc tacacaggcg
     1861 aagcccatgt gtctctagga acgatgttga gagaccaggc tattattatc aatggtttgt
     1921 ctaaatcgca tgccatgaca ggttggcgtt tggggctgat tttcgctcct gcggccttca
     1981 cagcccagtt aatcaagagt caccaatact tggtcactgc cgcaaatacc atggcgcaac
     2041 atgctgcggt agaagccttg acggctggta aaaatgatgc ggagcccatg aagaaggaat
     2101 atatccaacg tcgggactat atcatcgaaa aaatgactgc tcttggtttt gagattatca
     2161 aaccagacgg tgccttctat atttttgcta aaattccagc gggctacaat caagactcct
     2221 ttgcttttct gaaggatttt gctcagaaga aggccgttgc ctttatccct ggtgcagcct
     2281 ttggacgtta cggggaaggc tacgtccgcc tatcttatgc agccagcatg gagactatca
     2341 aagaagccat gaaacgactt gaggagtaca tgagagaagc atgattcagt ctatcacgag
     2401 tcaaggcttg gtgctttaca atcgcaattt tcgtgaggat gacaagctcg tcaaaatttt
     2461 tacagagcag gttggcaaac gcatgttttt tgtcaaacac gctggtcagt ctaagctggc
     2521 gcctgttatt cagcccttgg tgctggcacg atttctcttg cgaatcaatg atgacggact
     2581 cagttacatc gaagactatc atgaggtcat gacttttccc aagattaata gtgacctctt
     2641 tgtcatggcc tatgcgacct atgtggcagc tcttgcagat gctagtttgc aggacaatca
     2701 gcaggatgct cccttgtttg cttttttgca aaagactttg gagttgatgg aagcaggctt
     2761 ggattatcag gttttgacca atatttttga aattcaaatt ttgactcgat ttggaatcag
     2821 cctcaatttt aatgagtgtg tcttctgcca tcgggttggt caggcttttg acttttcttt
     2881 caaatatgga gcctgcctct gtccagagca ttatcatgag gataagagac gttgtcatct
     2941 caatcccaat atcccctatc tgctcaatca atttcaagct attgattttg agactttgga
     3001 gaccatttcg ctcaagcctg gaatcaagca agagctacgc caatttatgg atcaattata
     3061 tgaagagtac gttgggattc acctaaaatc aaagaaattt attgattccc tagcagactg
     3121 gggacaatta ctaaaagagg aaaagaaatg aaaaaaatcg cagtagatgc catggggggc
     3181 gattacgcac ctcaggccat tgttgagggt gtcaatcaag ccctatctga cttttcagat
     3241 atcgaggttc aactttacgg agatgaagct aaaatcaagc aatatctgac agcgacagag
     3301 cgcgtcagca ttatccatac ggatgagaag attgattcgg atgatgaacc tacgagagct
     3361 attaggaata agaaaaatgc cagtatggta ttggcagcca aggctgtcaa agatggtgaa
     3421 gcagacgctg tcctttcggc tgggaataca ggtgccttgt tggcagcagg attcttcatc
     3481 gtgggtcgta tcaagaatat cgaccgtcct ggactcatgt ctaccttgcc taccgttgat
     3541 ggaaaaggtt ttgacatgct agaccttggt gccaatgcag aaaatacagc ccagcacctc
     3601 catcaatatg cggttctagg ttccttctat gctaaaaatg tccgtggcat tgcgcaacca
     3661 cgcgttggtt tgctcaacaa cggaacagag agtagcaagg gcgacccgct tcgtaaggaa
     3721 acttatgaat tactggcggc tgatgaaagt ttgaacttta tcggaaacgt ggaagcgcgt
     3781 gatttgatga atggcgttgc agatgttgtt gtggcagatg gtttcacggg aaacgctgtg
     3841 ctcaaatcca tcgaagggac agctatggga atcatgggct tgctcaagac agctattaca
     3901 ggtggtggtc ttcgagcgaa actaggtgcc ctccttctca aggacagcct cagaggtttg
     3961 aaaaaacagc tcaattattc agatgttggt ggagcggtct tgtttggtgt taaggcacct
     4021 gttgtcaaga ctcatggctc aagcgatgcc aaggctgttt atagtacgat tcgtcagatc
     4081 cgtaccatgc tagaaacaga cgtggttgcc cagactgcgc gtgaattttc aggagaataa
     4141 aagagatgac agaaaaagaa atttttgacc gtattgtgac cattatccaa gagcgacagg
     4201 gagaggactt tgtcgtgaca gaatccttga gtctgaaaga cgatttggat gcggattctg
     4261 ttgacttgat ggagtttatc ttgactctgg aagatgaatt tagtatcgaa atcagcgatg
     4321 aggaaattga ccaactccaa agtgtaggag atgtggttaa aatcattcaa ggaaaatagc
     4381 aatcggagtt ccaagtcaac ggaagtagat ggtttttaga aat
//
LOCUS       DAVBBS010000092         4269 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVBBS010000092 DAVBBS010000000
VERSION     DAVBBS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.092"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..124
                     /locus_tag="VQU32_002025"
     CDS             <1..124
                     /locus_tag="VQU32_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET2473415.1"
                     /translation="YSPEYNPIEKTWAHIKKHLKKVLPSCNTFYEALLSCSCFN"
     gene            complement(117..290)
                     /locus_tag="VQU32_002026"
     CDS             complement(117..290)
                     /locus_tag="VQU32_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HET2473416.1"
                     /translation="MNIKKRVLSAGLTFASVLLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYTDNIVN"
     gene            complement(448..2055)
                     /locus_tag="VQU32_002027"
     CDS             complement(448..2055)
                     /locus_tag="VQU32_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HET2473417.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            2208..3692
                     /locus_tag="VQU32_002028"
     CDS             2208..3692
                     /locus_tag="VQU32_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HET2473418.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFLNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGISPADRKVAVVARQKAEETGGPALALELPNGDIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            3787..4269
                     /locus_tag="VQU32_002029"
     CDS             3787..4269
                     /locus_tag="VQU32_002029"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HET2473419.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
BASE COUNT         1241 a          978 c          811 g         1239 t
ORIGIN      
        1 ctactcacct gagtacaatc ctattgagaa aacatgggct catatcaaaa agcacctcaa
       61 aaaggtatta ccaagttgca atacctttta tgaggctctt ttgtcttgtt cttgtttcaa
      121 ttgactatat tatcagtgta atagtctaat agatagttaa atgtagttgg atttccacta
      181 aaggttgatg agtaagtttt tgtatctgaa cctgattggc cgcaagcagc taaaagcaaa
      241 acagatgcaa aagtcaggcc tgcactaagg acacgcttct ttatgttcat cttctttctc
      301 cttaatagtg ggaatttgta aagttaattg aatttcgaga atgaaagttt ataaaccttg
      361 gttataaaaa acaaaggatt tctgctttta tacagtcctc ccttgttttt atatgatttc
      421 aattttaaat ttttctgcaa aaaatattta tagtaattcc acacagaaag catcccatgg
      481 aactaagatt tgtttttcaa agacttcttg agctagggtg ttttcaatca agacagattt
      541 gacttttcct tctactgtca agtcttgctc ttcattggac aagttagcca caactaggaa
      601 gcgacggtcg ccatccttac gtatataagc aaagacctta tcagccgtat caagcaattc
      661 aaagtcagct cgaactagcc agctattctc cttgcgaatt tggaccagtt tctgataggt
      721 atagaaaata gaatctggat ttgccagcgc ttcttggaca ttgatcatct cgtaatttgg
      781 attaaccgcc aaccaaggtt gacctgttga gaaaccagcg tttttgctct cgtcccattg
      841 cataggggta cgggcattgt cacgtccaat aacacggata ctgtccatga tttcttcaat
      901 cggaacacct ttttcaagag cctcacgcgc atagttgaga gattcaatat cttctacttg
      961 atccagtgtt tcaaacggat agttggtcat cccaatctcc tcaccttggt agatataagg
     1021 agttcctctc ataagatgaa gcaagattgc aaaggctttg gcagattttt cgcggtattc
     1081 ttggtcattt ccccagattg agacaatacg agggaggtca tggttgttcc agaagaggga
     1141 attccagccg tcctcaactc ctaactctgt ctgccatttg ttgaagattt cttttaactt
     1201 agcgatattc agctcttttt gatagtgcca tttaggctga ccttcctgat actgaagacc
     1261 gatatgttca aactggaaga ccatagacaa ttcttgcccc tttggatcag agtagagctt
     1321 ggcaatctct ggcgttgctc cccaagtctc ccctactgtc aagagatcct tatctccaaa
     1381 ggtcgcctga ttcatttcct tgagataggg atggagcata ggaccattat tgactacctt
     1441 ctcgtcagga attttgccaa tcatgtcaat aacatccata cggaaaccac caataccctt
     1501 atcaatccag aagttcatca tctcataaat tttctggcga agtttttcat tttcccagtt
     1561 gagatccggc tgtttcttgc tgaaaaagtg gagatagtat tgacctgact tttcatcgta
     1621 ttcccaagca gacccactaa agatagaatc taaatcgtta ggctcatccc gccagatata
     1681 gtagtctcgc tcagggctat taggattttc acaggcctct acaaaccagg catgctcatc
     1741 cgaggtatga ttgaccacca agtccatgat gatacggata tcacgcttct tagcttctgc
     1801 aatcagctga tccatgtcct ccatggttcc aaaaatagcc gcaatcgctt gataatcagc
     1861 aatatcgtag ccattatcat ccatagggct gtcataaacg ggagaaagcc aaattgctgt
     1921 gattcctagc ttggctagat agtccaactt actggtaatt cctggcaaat cgccaattcc
     1981 atctccatta ctatccataa aactctttgg atagacttga tagactacgg cattatgcca
     2041 ccatttttct tgcatctgtt tacctcatta ttttcataat ctatagtcta tgatagcact
     2101 ttttaaaatt ttgtgcaagc gtttgcttaa tctttttaat ttctttttta gctccgtagt
     2161 ttcctaactt ccaatttttt attataatag agtccagagg taaaaaaatg aaaaaacaag
     2221 cttttagttc tgaacagtat ttgaatttac aacgcgacca tattttggag cgcattaacc
     2281 aatttgacgg caagctctac ttagagtttg gcggtaaaat gttagaagat ttccacgctg
     2341 ctcgtgtcct tcctggttat gaacctgaca acaaaatcaa gctcttgcaa gaattgaaag
     2401 agcaggttga ggttgtgatt gccattaatg ctagcaacat tgaacattcc aaagcacgtg
     2461 gcgacttggg catttcttat gaccaagaag ttcttcgttt gattgacaaa ttcaatgaat
     2521 tagggatttt tgttggttcc gttgtcatta cacaatacgc tggccaaccc gctgcagatg
     2581 ccttcctcaa ccaacttgag aaaaacggaa ttgattctta tcttcattat ccaatcaaag
     2641 gatatccgac ggatatggat cacatcattt ccccagaagg catgggcaaa aacgactaca
     2701 tcaaaaccag tcgcaacttg attgtcgtaa ccgctcctgg acccggttct ggaaaattgg
     2761 caacgtgtat gtccaatatg taccacgacc aaatcaatgg catcaagtct ggctacgcta
     2821 aatttgaaac cttccctgtt tggaatcttc cccttcatca tccagttaac ttggcttatg
     2881 aagctgccac agctgacctt gatgatgtca acatgattga ccccttccat cttcaaacct
     2941 atggagaaac cactgtcaac tacaaccgtg atatcgaaat cttcccagtg ctcaagcgca
     3001 tgttggaacg tattctcgga aaatcaccat acgcttcacc gacagatatg ggtgtcaaca
     3061 tggttggttt cgctattaca gatgacgagg ctgctgtcga agcttctaaa caagaaatca
     3121 tccgccgtta ctatcaaaca gttcttgact tcaaagctga aaaagttggc gaagctgccg
     3181 tcaagaaaat tgagttgctc atgaacgacc tcggtatctc acctgcagac cgtaaggttg
     3241 ctgtcgttgc gcgccaaaaa gcagaagaaa ctggcggacc agccctagct cttgaattac
     3301 caaatggtga cattgtcact ggtaaaaact cagaactctt tggccctaca gccgctgcct
     3361 tgatcaacgc catcaaaaaa tcagctgaca tcgctaaaga agtaaaacta atcgagcctg
     3421 aagttgttaa gccaatccaa ggacttaaaa tcgatcatct cggtagccgc aatccacgcc
     3481 ttcattcaaa tgaaatcctg attgcacttg ctatcacagc tacagaaaat cctgatgctg
     3541 cccgcgctat ggaagaactc ggcaacctca aaggaagcga agcccactca accatcatct
     3601 tgactgatga agacaagaat gtccttcgta aactgggtat caacgtaacc tttgacccat
     3661 actaccaata cgaccgctta tatcgtaagt aaagatttca acctctccag cctcggagag
     3721 gttttctctc tgtctcagga gcaagcttta tgatataatg agagaagaaa actgaaagga
     3781 tttaccatgt caaaagaagt tattgtcgaa agttttgaac ttgaccacac cattgtcaaa
     3841 gcaccctatg ttcgcttgat tggggaagaa acaggaccaa aaggagacat catctccaat
     3901 tatgatattc gcttggtgca accaaacgaa gactctatcc ctactgccgg ccttcacact
     3961 atcgagcacc tcttagccaa actcatccgt acccgcattg acggcatgat tgactgttca
     4021 ccatttggtt gccgcacagg cttccacatg attatgtggg gacgtcacac cagtgctaaa
     4081 atcgcagctg ttatcaagga ttcgctcaag gaaatcgctg aaactactac ttgggaagat
     4141 gtcccaggga caaccatcga atcttgcgga aactacaagg accacagcct cttctctgct
     4201 aaagaatggg caaaactcat cttggaacaa gggatttcag atgatgcctt tgaacgtcat
     4261 gtgatttaa
//
LOCUS       DAVBBS010000093         4116 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVBBS010000093 DAVBBS010000000
VERSION     DAVBBS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.093"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..776
                     /locus_tag="VQU32_002030"
     CDS             <1..776
                     /locus_tag="VQU32_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473420.1"
                     /translation="DYSIVEEKLDWQSKLSKIYPNVEFIKIEKLKLILLCKYRNQIID
                     LEDDKKRLLLLKLLYLTSKQFCYDEFKGAKFGCMSFFSHYQGYMSRLDSLDSDKFVSV
                     KQRIVSSISKLVEQYSDHIINLDRTLVDSLSDTLENQLFDDWDYILSEITTYSELLAK
                     SGLLYNSLDYKIDNVKKEVAKLSDFHQSLFKIEGFSDFFDSWQFQRYRSLVNQVYEIF
                     SMLQVSSVERTVLCGALSTIVFSKSNFNYQAFFEYLKDT"
     gene            805..1713
                     /locus_tag="VQU32_002031"
     CDS             805..1713
                     /locus_tag="VQU32_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473421.1"
                     /translation="MDYVDVILDEYGSVFLIKDHIKLKINNLELYRYFSSFGMSHLQF
                     RKEELPGKLKQFDFEKNKSDSYFSKKSSLIDFIKRNYFYDIKLYSDNPKNPIESWKNK
                     TEKIKIAKSTEILWSEVANFSSISKSPYYCLLYNLKNTENYILIISKTYFSAIQKMVS
                     YNLNNYFELEDVCCKSIVSKRELIENSININKSKMKLLNVCEGSKGYTRRVFKYNNIF
                     VGQLEFHSNIIFSEIALNELSIVKLLDSVESVLSEQLFGKLNWDNLFVVLSDDFNDYL
                     DINESQKYYSNGSLRGLELGIFMGGV"
     gene            1715..3613
                     /locus_tag="VQU32_002032"
     CDS             1715..3613
                     /locus_tag="VQU32_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HET2473422.1"
                     /translation="MRIIEIVDVEDQLLQLLSAHTNIDIRAQELSTLDPDSKVDLIIY
                     LKSRRDIENINNIIIKSPNRNIIVIISAFDIIYALYFPKSTFRPCLNCLLLKLKDNNP
                     EHNLLLFLKYGTLPSKHFNLDNRLKSNLILFNEKLDPKVEEYSIEGVNTTNFTFHFSC
                     SCENSGYDMSERMADILLGEGKKLSPKNYRLKKSEHLFDFTRYLDRNFGFFHHSYDEK
                     ISEYAPLHAVEINVDAGYESGWGRTFMTEAATKSAFLESLERYCNSRNRRYKKLIRGC
                     FSDLSKKYNTLNPVDFCLSNKEYRDSSYKFHDFDSEKNYDWIFGKNITTGKICLLPSQ
                     LVFYKDFSKSEENERFVYETSNGCALGSSIEEAIFYGILEIIERDNFLLTWYLKEEVK
                     QLSWESLPSRLRIINERLKEDNCELKLFNITKEVGIPVIWALYINHNENASIRSFSAA
                     GCHINPLSAIESAIFEVITSVPTFERLIEVRNKESFIEEIQNNFSLIRDIDDHILYHA
                     HADYKNKMPFLFKNNYSEYQNEFDENRIFNTYSHATLTSDLQALIKIIEKKFGDIYVV
                     NISDKVSEKFGLYCVKVLIPGLLNMSFGHSNRRVNIERIKNYVDYRGGNVNSITLNSI
                     NHLPHPFP"
     gene            3637..>4116
                     /locus_tag="VQU32_002033"
     CDS             3637..>4116
                     /locus_tag="VQU32_002033"
                     /inference="COORDINATES: protein motif:HMM:NF025394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HET2473423.1"
                     /translation="MKRDWKSKHLFYHDVQQFDKLVIYLSKFMDKLVNQNQIEKYFYI
                     RYWEGGPHIRLRYLVRDTQNFETIDSMINKYAYKFWENNPPMKLLNKTDYKKIYNESI
                     DNNGKMDRKLRKNATIENIAYIPEIERYGGEEALIVSEDLFCICSQVSTALLNVVDNY
                     "
BASE COUNT         1539 a          443 c          712 g         1422 t
ORIGIN      
        1 cagattactc aatagtggaa gaaaaattag attggcaaag taaattatct aaaatttatc
       61 cgaatgttga atttataaaa attgaaaaat taaaattgat tttgttgtgt aaatatagaa
      121 atcaaattat tgacttagag gatgataaaa aaagattgtt actattgaaa ttattatatt
      181 tgacttcaaa gcaattttgc tatgatgaat ttaaaggagc gaaatttgga tgtatgtcat
      241 ttttttcaca ttatcaaggt tatatgagca gattagattc actcgactca gataaatttg
      301 tttcggttaa acagaggatt gtttcctcta tttctaaatt agttgagcaa tattcagatc
      361 atattataaa tctggataga actttagttg attctctaag tgataccctt gagaatcagc
      421 tatttgatga ttgggattat atattaagcg aaataactac atatagcgaa ttattagcaa
      481 aatcaggatt gttatataat tcgctagact ataagattga taatgtaaaa aaagaagtgg
      541 ccaagctaag tgactttcac caatctttat ttaaaattga aggattttca gatttttttg
      601 atagttggca atttcaaaga tataggtctc tcgtgaacca agtttatgaa attttttcaa
      661 tgttacaagt aagtagtgta gaaagaactg tgttgtgcgg agctctaagt actattgtct
      721 ttagtaagtc aaattttaac tatcaagctt tttttgagta tctaaaagat acgtagaatt
      781 cgattttaaa ggaggctgaa atatatggat tatgtagatg taatattgga tgagtatggg
      841 agtgtttttc tgataaagga tcacataaaa ctaaaaatta ataatctaga actatatcgg
      901 tattttagta gctttggaat gagtcatctt caatttagaa aagaagagct tccaggaaaa
      961 ttaaagcagt ttgattttga aaaaaataaa agtgacagtt atttttctaa gaaaagtagt
     1021 ctgattgatt ttattaagag aaattatttt tatgatatta agttatattc tgataatccg
     1081 aaaaatccga tagagtcttg gaaaaataag acagaaaaaa ttaagattgc taaaagtaca
     1141 gaaattcttt ggagtgaagt agcaaatttt tcgtcaattt ctaaatctcc ttactattgc
     1201 ttgttatata atttaaagaa tactgaaaat tacatattga taatcagtaa gacatatttt
     1261 tcagccatcc aaaaaatggt ttcgtataat ctaaataatt attttgaatt agaagatgtt
     1321 tgttgtaaaa gcatagtgag caagcgagaa ttgatagaaa attcaattaa tattaacaaa
     1381 agcaaaatga aactgctaaa tgtttgcgaa ggtagtaaag gttacacaag aagagtattt
     1441 aagtacaata atatttttgt gggacaattg gaatttcact ctaacataat cttttcagaa
     1501 attgctttaa atgaattaag tattgttaag cttttagatt ctgttgaaag tgttttatct
     1561 gagcaactat ttggaaaatt aaattgggat aatttatttg ttgttttatc agatgatttt
     1621 aatgattatc tggatataaa tgaatctcaa aaatactact caaatggttc tttgagagga
     1681 ctggagcttg gaatctttat gggaggggtt taaaatgaga ataattgaaa ttgttgatgt
     1741 tgaggaccaa cttttacaac ttttaagtgc acataccaat attgatatta gagcacaaga
     1801 gttatcaaca cttgacccag attcaaaagt tgatttgatc atatacttga aatctcgacg
     1861 tgatattgaa aacataaata atattattat taaatcacct aacaggaata tcattgttat
     1921 aataagtgct tttgatatta tatatgcatt gtattttcca aaatcaacat ttagaccttg
     1981 tttgaactgt ctattattaa aacttaaaga taacaaccct gagcataatc tattattatt
     2041 cctgaaatac ggaacattac ctagtaaaca ttttaatctt gataataggt taaaatcaaa
     2101 tttaatacta tttaatgaga agttagaccc taaggtagag gagtatagta ttgagggggt
     2161 aaatactaca aattttactt tccattttag ttgtagctgt gaaaatagtg gttacgatat
     2221 gtcggaaaga atggcagata ttctacttgg tgaaggaaaa aaattatctc ctaaaaatta
     2281 tcgtttaaaa aagagtgagc atctattcga ttttactaga tatctagata gaaattttgg
     2341 attttttcat cattcatatg atgaaaaaat ctccgaatat gcgcctctcc atgcagtaga
     2401 gattaatgtt gatgctggtt acgagtcagg ctggggaagg acttttatga cagaggctgc
     2461 aacaaaatct gcttttttag agtcattaga gagatactgc aactctagaa atagacgcta
     2521 taaaaaatta attagagggt gtttttctga tttatctaaa aagtacaata cattaaatcc
     2581 tgttgatttt tgtctttcta ataaagaata tagagatagt tcctataaat ttcatgattt
     2641 tgattctgag aaaaattatg attggatatt tggtaaaaat ataactaccg gaaaaatttg
     2701 cttattgccc tcgcaactag tattttataa agactttagt aaaagcgaag aaaatgaaag
     2761 gtttgtttat gaaacatcaa atggatgtgc tttggggagt agtattgagg aagctatttt
     2821 ttatgggatt ttagagatta ttgaaagaga taatttttta cttacatggt atttaaaaga
     2881 agaagtaaaa caattatcat gggaatcttt accatctcgc ctacgtatta ttaatgaaag
     2941 actaaaagaa gataactgtg agctcaaact ttttaatata actaaagaag tgggcatacc
     3001 agtaatttgg gcattgtata taaatcataa tgaaaatgct agtattcgaa gtttttcagc
     3061 tgcaggctgt catattaatc ctttaagtgc aattgaatca gcaatttttg aagtaataac
     3121 ttctgtgcca acttttgaaa gattaattga agttagaaat aaagagtcat ttattgagga
     3181 aattcagaat aatttttctt taatacgaga tattgacgat catatattgt atcatgctca
     3241 tgcggattat aaaaataaaa tgcccttttt atttaaaaat aattatagtg aatatcaaaa
     3301 tgaatttgat gagaatagaa tttttaatac ttattcacat gcaacgctta catcagattt
     3361 acaggcacta ataaaaatta ttgaaaaaaa atttggagat atatatgtag tgaatataag
     3421 tgataaagtt tccgaaaaat ttggacttta ttgtgttaaa gtgttgattc ctggacttct
     3481 taatatgtca tttggacatt caaatcggcg tgtaaatata gagaggataa aaaattatgt
     3541 agattataga gggggaaatg taaatagtat tacattgaat tccataaacc atcttcccca
     3601 tccatttcca taagattata tataaaggaa taataaatga aaagagattg gaaatcaaag
     3661 catttatttt atcatgatgt tcaacaattt gataaattgg taatctattt atcaaaattt
     3721 atggataaat tagttaatca gaatcagatt gaaaaatatt tctatattcg ttattgggaa
     3781 ggggggcctc atattcgatt aagatatttg gtgagggata cacaaaattt tgaaacaatt
     3841 gatagtatga taaataagta tgcttataaa ttttgggaaa ataatcctcc aatgaagtta
     3901 ttaaataaga ccgattataa gaaaatttat aatgagagta ttgataacaa cggaaagatg
     3961 gatagaaaat tgaggaaaaa tgcgactatt gaaaacattg cctatatacc ggaaatagag
     4021 agatatggcg gggaagaagc tctgatagta agcgaagatc tattttgtat ctgtagtcag
     4081 gtttcaactg cgttacttaa tgtggtagat aactat
//
LOCUS       DAVBBS010000094         3939 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVBBS010000094 DAVBBS010000000
VERSION     DAVBBS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.094"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            98..1357
                     /locus_tag="VQU32_002034"
     CDS             98..1357
                     /locus_tag="VQU32_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HET2473424.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQIQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    1433..1568
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1607..2107
                     /locus_tag="VQU32_002035"
     CDS             1607..2107
                     /locus_tag="VQU32_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET2473425.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            2183..2551
                     /gene="rplL"
                     /locus_tag="VQU32_002036"
     CDS             2183..2551
                     /gene="rplL"
                     /locus_tag="VQU32_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET2473426.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKTKLEEAGASVTLK"
     gene            2738..2908
                     /locus_tag="VQU32_002037"
     CDS             2738..2908
                     /locus_tag="VQU32_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473427.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            2908..3156
                     /locus_tag="VQU32_002038"
                     /pseudo
     CDS             2908..3156
                     /locus_tag="VQU32_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            3170..3510
                     /locus_tag="VQU32_002039"
                     /pseudo
     CDS             3170..3510
                     /locus_tag="VQU32_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3503..3883
                     /locus_tag="VQU32_002040"
     CDS             3503..3883
                     /locus_tag="VQU32_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET2473428.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
BASE COUNT         1260 a          700 c          842 g         1137 t
ORIGIN      
        1 agatagaact gacgaagtca gtaaccacac ctacggcaaa gtgaatctga agtggtttga
       61 agagagtaca acttgtcttt tagaaaagga gcctataatg aaagtctttc agcatgtaaa
      121 tatcgtgact tgtgatcaag atttccatgt ttatcttgat ggaatcttag cagtcaagga
      181 ttctcaaatc gtctatgtcg gtcaagataa gccagcgttt ttagagcaag ctgagcagat
      241 tatagactat cagggagctt ggattatgcc tggtttggtc aattgtcaca cccattctgc
      301 aatgacaggt ctgagaggga tccgagatga cagcaatctc catgaatggc tcaatgacta
      361 tatctggcca gcagaatctg agtttactcc cgacatgact accaatgcgg tcaaagaagc
      421 cctgacagag atgctccagt caggaacaac aacctttaac gatatgtata atcccaatgg
      481 tgtggatatc cagcaaattt atcaggtggt gaaaacttcc aagatgcgtt gttatttttc
      541 tccgactctc ttttcttcag agacagaaac aactgctgag actataagca gaactcgatc
      601 catcatagac gaaatcttaa aatataaaaa tccaaatttc aaggttatgg tagcacctca
      661 ttctccgtat agctgcagta gagacttgct ggaagcgagt ttggaaatgg caaaagagct
      721 aaatattccg ctccatgtcc atgtggcgga gaccaaggag gagtcaggaa ttatcctcaa
      781 acgatacggc aaacgccccc ttgcttttct tgaagaactg ggttatttag atcatccgtc
      841 cgtatttgct cacggggtcg aattaaacga gagagaaatt gaacgcttgg catcttctca
      901 agtggctatc gcccacaatc ctatcagtaa cctcaaactg gcatcaggaa ttgctccaat
      961 tatccagatc caaaaagcgg gagtagcagt cggaattgcg actgactcgg ttgcttccaa
     1021 taacaatcta gatatgtttg aggaaggaag gactgcagct cttcttcaga agatgaaaag
     1081 tggggatgcc agccagtttc caatcgaaac agctctcaag gtactgacaa tcgaaggggc
     1141 taaggtcctt ggaatggaaa atcagatagg aagtctggaa gtcggcaagc aagcagattt
     1201 tctggtcatt caaccacaag ggaaaattca tctccaacct caggaaaata tgctgtctca
     1261 cctggtttat gcagttaaat ctagtgatgt agatgatgtt tatatcgccg gagaacaggt
     1321 tgttaagcaa ggtcaagtcc tgacagtaga actttaaaag aaaaatcacg aaaaatttta
     1381 aaaaaagttt gcaaaaatct tgcattcttt ttttgactat gctatactta tatacggttt
     1441 aaaaaaactg cctaagacag taggggagct cgactcataa atatcctacc gaggacaaga
     1501 cgtatcatgt aaaaagaagc gtattgtact ttcgtgtcta ggtttgggcg cgtttttctt
     1561 tttgaaaaat tccccaagca aaataattac ggaggtgaac acactaatga gtgaagcaat
     1621 tattgctaaa aaagcggaac tagttgacgt agtagctgaa aaaatgaaag ctgctgcatc
     1681 tatcgtcgtt gtagacgctc gtggtttgac agttgagcaa gatacagttc ttcgtcgcga
     1741 gcttcgtgga agcgaagttg agtataaagt tattaaaaac tcaatcttgc gtcgtgcagc
     1801 tgaaaaagct ggtcttgaag atcttgcatc tgtatttgtt ggaccatctg cagtagcatt
     1861 ttctaatgaa gatgttatcg caccagcgaa aatcttgaac gacttttcta aaaacgctga
     1921 agcacttgaa attaaaggtg gtgcaatcga aggcgctgtc gcatctaaag aagagattct
     1981 tgcacttgca actcttccaa accgcgaagg acttctttct atgctccttt ctgtacttca
     2041 agcgccagtg cgcaacgttg ctcttgcagt caaagcggtt gcagaaagca aagaagacgc
     2101 ggcttaatct taagctacac agcgtagcct agctacgaaa aaaactatta taaaatttaa
     2161 aacttatttg gaggaaataa caatggcatt gaacattgaa aacattattg ctgaaattaa
     2221 agaagcttca atccttgaat tgaacgacct tgtaaaagct atcgaagaag aatttggtgt
     2281 aactgcagct gctcctgtag ctgttgctgc agctgatgca gctgatgctg gtgctgctaa
     2341 agattcattc gacgttgaat tgacatctgc aggcgacaaa aaagttggcg ttatcaaagt
     2401 tgtacgtgaa atcactggtc ttggtcttaa agaagctaaa gaacttgttg acggtgcacc
     2461 agcacttgtt aaagaaggcg ttgcaactgc agaagctgaa gaaatcaaaa ctaaattgga
     2521 agaagctgga gcttcagtta ctcttaaata atagagtatc gcaatttgat tgcggaatcc
     2581 ctatttacca gtctttgaga gcgataagcg attgatagaa actgataaat tgatttggtt
     2641 tcctgccaaa aattctttgg caggattttt gttttaataa actttcgggg gtagacacta
     2701 gattgtctac cccttttttt gtgtttcgga ggtaattatg caaaaggaag gacagtcatc
     2761 acgtcaattc gtgaagagtc atctcatgac tatcatggga gttgatattg taaaagcaac
     2821 tcagttagtt gatgaaatgg aacaagtagg tttaatccga tttgatgaat ttggaaatgt
     2881 tggaatttta gtcttggagg gacaataatg aagtgtatta ccgctaatca atatcagaca
     2941 tcagagcgtt attataagct ccccaaactt ttatttgaga gtgaacggta taagaatatg
     3001 aaactggagg ttaaggtggt ttactcagtt ttaaaagatc ggttggagtt gtctttgagc
     3061 aaaggttgga ttgatgagga tggggctatt tatttgattt attccaattc aaatttgatg
     3121 gcacttttag gctgttcaaa gtcaaaatta ctctccatgt gagtttgaag tgacattttt
     3181 agatgattac cataaaaaac ataactaccc actattttac gaatcctatc ttcaaaacgt
     3241 tatggaattc cttgaaagtc aagacataaa gaatggggtt gatgcctttg tagatgatca
     3301 tcaaaatctc gtttttgttt tatatggaca aggctatcga gccgagggaa aagagggaat
     3361 acttacaacc caagtaactg taaaagctta tgatgaagac aagaaaccga ttaacttcgc
     3421 aaatttatta gattccttaa tttattagat tccttaatcg tgtcagaata tcaaatggaa
     3481 ccgaatcttt gggaggtctc ccatgattga tctctatcta agtaaaaata gacaaagaaa
     3541 tcaacttctt ttagatttct tccaaaacta tggcatagag gtatcttgtc attcagtttc
     3601 tgaaatgaca aaggacaaat taattgagat gatgagctat tcttcagatt gttttgagtt
     3661 tttatctccc aatttattac gatttaagaa ccgtgacaat ctaagattaa cggatttcgt
     3721 tgagataatt ttaaaaaatc ctgaactaac catcagactt cctcttgcgg tttcaaataa
     3781 gcgagtttat ccaagtctga atttggaaga ggctagagct ctattgccaa gagatacgaa
     3841 acaattgatt tacatggcgc aaacacatta tttatctaac taaaggagaa gcaatatggc
     3901 tgaacgattt tgggagaact tgtccattat tttggctga
//
LOCUS       DAVBBS010000095         3719 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVBBS010000095 DAVBBS010000000
VERSION     DAVBBS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.095"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(119..970)
                     /gene="rfbD"
                     /locus_tag="VQU32_002041"
                     /pseudo
     CDS             complement(119..970)
                     /gene="rfbD"
                     /locus_tag="VQU32_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647909.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
     gene            complement(1036..2085)
                     /gene="rfbB"
                     /locus_tag="VQU32_002042"
     CDS             complement(1036..2085)
                     /gene="rfbB"
                     /locus_tag="VQU32_002042"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET2473429.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLVAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGIYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQATD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            complement(2095..2691)
                     /locus_tag="VQU32_002043"
     CDS             complement(2095..2691)
                     /locus_tag="VQU32_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HET2473430.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     VLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENLEEADVSEADKHHPLLKDVKPLKKEDLE"
     gene            complement(2692..3549)
                     /gene="rfbA"
                     /locus_tag="VQU32_002044"
     CDS             complement(2692..3549)
                     /gene="rfbA"
                     /locus_tag="VQU32_002044"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET2473431.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLHRFQELLQDGSEFGIKLSYAEQPSTDGLAIGEEFISDDSVALIL
                     GDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEKPEYP
                     RSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRGFAWL
                     NTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLALAQPLKKNEYGQ
                     YLLRLIGEA"
BASE COUNT         1041 a          860 c          666 g         1152 t
ORIGIN      
        1 ctgagccaaa gctatgcacc aaagagacca gcaccaacaa caagataatt gtacatctta
       61 agctcctttg gagttcatta tctgcctctt ttattagact agaaaatcat tctactactt
      121 atctcacttc ttgtttgtaa aattcttgca atgcatcttg ccaagttgga ataacaaatc
      181 cagtagcttt ggctttggcc aggctcatcg ttgagtttag cggacgttga gctttggctg
      241 gaaattgact ggaatctact ggcttgactt cgacatctgt atctttcaaa atttcaactg
      301 caaaatcata ccatgttgtg tcttctgtcg catcatttga caaatgataa taaccaaatt
      361 ccttacgatt ttcagctagg tagatcatga attcagccaa ggtacgagtc caagtcggac
      421 gaccgtactg gtcatttaca actgttaaag tcttatgagt tttcgcaaga ttttgcatgg
      481 taaaaacgaa gtttttgcca taatttccaa atacccaggc agtacggata atatagaaat
      541 tagacacatg cttctcaact aactcttccc ccatacgctt agtgcgtcca tattctgtct
      601 gtggatctgg tcggtcatca acttcccact cttgtccaac tggtttctta ccgtcaaaga
      661 cctagtccgt agaaatataa actagagttg caccatgctt ttcagatgct tttgcgacat
      721 tttttgtccc catcacattg atggcgaagt ccaactcttt tccttcatcc tctgctgcat
      781 caacagcggt gtaggctgca caatggtaga ctaaagtcgg tttcacctct tcaaaaactt
      841 tctcaaccat ttcttcattg gtaatgtcca tctcagccac atctactgcc acgtattctt
      901 cattacgttc atccaataaa tagcgaagtt ccgttcctaa ttggccattt gcccctgtaa
      961 ttaaaatcat ctctagacct ttctaatatg taactcttcc caatatagca ataaaaagca
     1021 gcgatttcct gctttttata ctgtaataat ctcctgagtc ttagcataat tggcttcaac
     1081 agcttctttt tctgctttcc accattcttg gttatctgta taccacttga ttgttgcctt
     1141 gagcccagct tcaaagttgg taaattcagg tttccacccc aactcatcac ggagcttgct
     1201 ggcatcaatc gcatagcgaa ggtcatgtcc tgcacggtca gtcacatgat cataggcatc
     1261 cgtagcttgt cccatttcct taaggataag ttccaaaact tccttattgt tcttctcacc
     1321 atcagcccca atcaagtagg tttcaccgat ttgccctttt gtcaagattg tccaaactcc
     1381 tgaagaatgg tcattggtat gaatccagtc acgaacgttc ttaccttcac cgtaaagttt
     1441 tggcttaatt ccacttagga tgttagtaat ctgacgtggg atgaattttt cgatatgttg
     1501 ataaggaccg tagttatttg aacagttgga aatcgttgcc ttgactccaa aagaacgcac
     1561 ccaggctttg acaatcaagt ctgatgcggc cttggttgat gagtacggag agcttgggtt
     1621 gtattttgtt tcagcagtaa atttctcacc tggtccttca ccatgacctg gcaaatcttc
     1681 tcgtaaaggg agatccccat aaacttcatc tgtcgataca tggtggaagc gaatatcata
     1741 cttacgagca gcttctaaaa gagtatagat tccaatgaag ttagtatgaa taaatggcga
     1801 tggatcattg agcgaattat cattgtggct ttccgctgca taatgaacga tagcatctgc
     1861 ttgagcaacc aacttgtcta ccaactccgc atcagcaatg tcaccaacaa ctaactcaac
     1921 acgattgcct aaaatttcct caatattcgc gcggtttcca gcataagtca acttatctag
     1981 gactgtcacg tgaacatctg gaaagttctc gtaaacataa tggacaaagt tagaaccgat
     2041 aaagccagct ccacctgtca cgataatatt tttgtattca gtcatattct ttccttattc
     2101 caaatcttct tttttcaaag gcttcacgtc cttaagtagg ggatgatgtt tgtctgcttc
     2161 agatacgtct gcttcttcca gattttccca ttcaatacca aggcttggat cagcgtagtt
     2221 cacaaaggca tacttgggtt tgagttcaag agcccagtaa tcattgacca gatagctata
     2281 tgacactgta tctgataaaa cttggaagcc attagctacg cctcgaggaa caaagattcc
     2341 cttgcttgca tcaattactg tctgataggt attcccaaag gtttcaccct cgcgtagatc
     2401 aacccaagaa cccagaactt tccctccatc tgctacagag atgtacttat cccaaggctc
     2461 tgcgtggagg cctcgaagga catttttacg tgagaaggat acattgtttt gcaattttcc
     2521 ttctgcaaag aaagactctg gaaatccaag tggaagcact ttttcctttt ggaaattttc
     2581 tttaaaccag ccacgattat ctccatgaac ggggatatca aactccaaca tgcctggaat
     2641 agcttcaacc ttgcgtgccg caagcgtctt accgaaaaaa ttatctgtca tctatgcttc
     2701 tccaatcaaa cggagcagat actgtccgta ttcatttttc ttaagtggtt gggctaaggc
     2761 caatacatct tctcgactga tatagcccat acggtaagca atttcttcta agtttgctac
     2821 ctgaacattt tgcatccgtt gcactgtttc gatgtactgt gaagcctcta gtaaactttc
     2881 atgagttcca gtattcagcc aagcaaagcc acgtcccata agctcaacag acaaatcacc
     2941 acgatctagg taagccttgt ttacatctgt aatttccagt tcaccacgag ggcttggttt
     3001 aatactcttg gcaatttcga caacatcgtt atcatagaaa tacagtcctg tcactgcata
     3061 gttggagcga ggatactccg gtttttcttc tatggaaata gcattcatgt ctgtatcaaa
     3121 ctcaaccaca ccaaaacgct ctggatcctt cacttggtag ccaaaaacag tcgcaccttt
     3181 ctctttcttg gctgcttttt gaagcattgt gctcagacca ggaccatggt agatattgtc
     3241 gcctaaaatc aaggcaacgc tatcgtcaga aataaactct tccccaattg ccaaaccatc
     3301 tgtacttggt tgctctgcat aagaaagttt gatcccaaac tcagatccgt cttgaagaag
     3361 ctcttgaaat cgatgtaaat cctgtggagt ggaaataatc aaaatatccc taatcccagc
     3421 caacatcaat gttgaaagtg ggtagtaaat catcggttta tcataaaccg gcataagttg
     3481 ttttgacgca gcgcgagtca aaggatataa acgtgtcccc gaaccacctg ctagaataat
     3541 acctttcata gtagggtacc tttctttgtt attttagagt caattactat taattgtgta
     3601 ctgctctaaa aagaaaacaa tcatcccatg ttttatatgg ctctataata tttgtagtgg
     3661 gtaaatccac tatagatatt atggagccta ttttattgta gaaaaaaagt cccataaga
//
LOCUS       DAVBBS010000096         3622 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVBBS010000096 DAVBBS010000000
VERSION     DAVBBS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.096"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            1..497
                     /locus_tag="VQU32_002045"
                     /pseudo
     CDS             1..497
                     /locus_tag="VQU32_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547411.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(577..777)
                     /locus_tag="VQU32_002046"
     CDS             complement(577..777)
                     /locus_tag="VQU32_002046"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473432.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDKTDEVSSKHGFEVVDETDEVS
                     NHTYGKATLTRIEEIFEEYKSS"
     gene            complement(755..949)
                     /locus_tag="VQU32_002047"
     CDS             complement(755..949)
                     /locus_tag="VQU32_002047"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473433.1"
                     /translation="MSSKKHPLGEKRNHTGSKLRQGFWMVFRLGVNNWGFKEIENKKP
                     SKNSIAGENEIILNENQRAN"
     gene            1011..2357
                     /locus_tag="VQU32_002048"
                     /pseudo
     CDS             1011..2357
                     /locus_tag="VQU32_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
     gene            complement(2757..3458)
                     /locus_tag="VQU32_002049"
     CDS             complement(2757..3458)
                     /locus_tag="VQU32_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HET2473434.1"
                     /translation="MYSGFTHYKYYRAEIIRKVQEPEFWKEVNLSDLEKIRLAIRDLL
                     QFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHYLKTHLDEESISKL
                     YHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREIIGLDRESANRIFSK
                     FLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQLLFQHQLPVLRNI
                     VQIIELINNRAGEAA"
BASE COUNT         1132 a          735 c          651 g         1104 t
ORIGIN      
        1 tcagccaaaa taatggacaa gttctcccaa aatcgttcag ccatattgct tctcctttag
       61 ttagatgaaa aggaggtaaa ccatctgatt aaatatgcca tgtttgataa ggaacagaag
      121 agaggctata aacagtcagc tagaaactta gcgaattggt actataatga caaggaggat
      181 agctatacac atcctgatgg ctggtattat cgttttcacc ataccaaaca tcagaaaaca
      241 cagacagact ttaaacaaga aatcaaggtt tactacgccg acgaacctga atcagcccct
      301 caaaagggac tgtatatgaa cgaaggctat caaaacttga aagctaaaga atgtcacctg
      361 tctttgggca gataaaggct tgtttgggtt acaagagatg taatctgagg ggcaagcgtc
      421 aagtgagaat tgacatggga ttggtactta tggccaataa cctcctaaaa tacaataaga
      481 gaacgactca aaattaaaaa gctaaagttc cacaattgga aatctctagc ttttttgtgg
      541 tcgagaacta ttttgtccca ggctctttgc tttatcctag gaagatttat actcttcgaa
      601 aatctcttca atccgcgtca acgtcgcctt gccgtaggta tggttactga cttcgtcagt
      661 ttcatctaca acctcaaaac catgttttga gctgacttcg tcagtcttat ctacaacctc
      721 aaaacagtgt tttgagcagc ctgcagctag tttcctagtt tgctctttga ttttcattga
      781 gtattatttc attttctcct gcaattgaat tcttgctcgg ctttttgttt tctatttctt
      841 taaaacccca attattcacc cctaatctaa aaaccatcca gaatccttgc cttagcttag
      901 atcctgtatg gtttcttttt tcacccaatg ggtgtttttt actagacaaa aaagaatttc
      961 ccctttatgg tataagtgta gaaaaaaaca caaaaagaaa ggaaactcac atgaacagtt
     1021 taccaaatca tcacttccaa aacaagtctt tttaccaact atctttcgat ggaggtcatt
     1081 taacccagta tggtggtctt atcttttttc aggaactttt ttcccagttg aaactaaaag
     1141 agcggatttc taagtattta gtaacgaatg accaacgccg ctactgtcgt tattcggatt
     1201 cagatatcct tgtccagttc ctctttcaac tgttaacagg ctatggaacg gactatgctt
     1261 gtaaagaatt gtcagctgat gcctactttc caaaattgtt ggaaggaggg cagcttgctt
     1321 cacagccaac cttatcccgt tttctttcca gaactgacga ggaaacagtc catagtttgc
     1381 gatgcctcaa ccttgaattg gtcgaattct ttttacagtt tcaccagcta aaccaactca
     1441 ttgtagatat cgattctacc catttcacaa cttatggcaa gcaagaaggt gttgcttata
     1501 acgcccacta tcgtgctcat ggctatcatc ctctttatgc tttcgagggg aagacaggtt
     1561 attgtttcaa tgcccagctt cgtcctggta atcgttattg ttctgaagag gcagacagct
     1621 ttatcacacc tgttttagaa cggtttaatc aacttctctt tcgaatggat agtggctttg
     1681 cgacccccaa attatacgat ttaattgaaa aaacagggca atactaactc ataaaactca
     1741 agaaaaatac tgttctgagc cgtcttggag acctttccct cccttgccca caggatgagg
     1801 acttaaccat cttgccccac tccgcctact cagaaactct ctatcaagca ggatcttggt
     1861 cgcacaagcg tcgtgtctgc cagttctctg aacgaaaaga aggaaacttg ttctacgatg
     1921 ttatttctct cgttacaaat atgacgagtg gaacaagcca agaccagttt cagctttatc
     1981 gtggacgtgg tcaagccgag aatttcatca aggagatgaa ggagggattt tttggcgata
     2041 aaacggatag ttcaacctta atcaaaaacg aagttcgtat gatgatgagc tgtatcgcct
     2101 acaatctcta tctttttctc aaacatctag ctggaggtga cttccaaact ttaacaatca
     2161 aacgcttccg ccatcttttt cttcacgtgg taggaaaatg tgttcgaaca ggacgcaagc
     2221 agctcctcaa attgtctagt ctctatgcct attccgaatt gttttcagca ctttattcta
     2281 ggattagaaa agtcaacctg aatcttcctg ttccttatga accacctgga agaaaagcgt
     2341 cgttaatgat gcattaaaga acagtcgaga tgaaaaaatc gtgtgacgca ccaagggagg
     2401 agtctgccct tttgaggaaa tctagcgagg aaaaacgata ctggaacagc agaaagtaaa
     2461 actgacctca tgaggaggaa gaaagtggct catgaggtca ggggttttgt aagttacatc
     2521 tagttgagag aggtatgaat gatttgggtt aaaatcaaag tagctctttt gttaataact
     2581 cgatcaacaa acatcgtggt acaagtatct actttgaaat ttatcaacca cttaacaact
     2641 gatactgtat tctaggaaaa cgatgacatt cttcctaata aaacttctca tatatagcat
     2701 aaatttctac tctttttaat tttaaatgag tctcagcatg gcaatcactt tagaatttaa
     2761 gccgcttctc cagctcgatt attgataagt tcaatgattt gaacaatatt acgaagtact
     2821 ggtagttggt gttggaagag tagttgaaca gaaccataag atttaaacgg ctcttgcgtt
     2881 aacactttcg tctctaaaaa accattttct acaatgtagt ctacaatcaa ttttacaaat
     2941 gaaatctgcc tggcattaag attctcatcc gacaaaaatt tagagaaaat acgattggca
     3001 gactctctat ctaagccaat aatctcacga accaatctcg gaattgcctt attttcataa
     3061 tgactttggt agtctgcttt actacctaat ttttcccaaa gcaatttttc aagtgcaagc
     3121 atatcatcag atgtcaactt tttattatgg tatagcttag aaatggactc ctcatccaga
     3181 tgagttttca aataatgctc aactttttca ttgtaagacc gaagatcgtt gacctgcaaa
     3241 aatgctgtgg tctcgtgaac agtggagagt atacgatctt caaagttaac atagtagggt
     3301 ttacggtctg ttttatccaa aaactgtaat aaatctcgaa tagcaagacg aattttttcc
     3361 aaatcagaca agttaacttc tttccaaaat tcaggctcct gtactttcct gataatttcg
     3421 gctctataat atttgtagtg ggtaaatcca ctatagatat tatggagcct attttattgt
     3481 agaaaaaaag tcccataaga tctataatga aaagcgacca aacaactcat tagaaagatt
     3541 catatggaac aattacattt tatcacaaaa ctactcgata tcaaagacac aaatatccaa
     3601 attatagatg tcgtcaatag gg
//
LOCUS       DAVBBS010000097         3262 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVBBS010000097 DAVBBS010000000
VERSION     DAVBBS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.097"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(216..827)
                     /gene="rpsD"
                     /locus_tag="VQU32_002050"
     CDS             complement(216..827)
                     /gene="rpsD"
                     /locus_tag="VQU32_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET2473435.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1022..2074)
                     /locus_tag="VQU32_002051"
     CDS             complement(1022..2074)
                     /locus_tag="VQU32_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET2473436.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVTKLEKGQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2071..2769)
                     /locus_tag="VQU32_002052"
     CDS             complement(2071..2769)
                     /locus_tag="VQU32_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2473437.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2936..>3262)
                     /locus_tag="VQU32_002053"
     CDS             complement(2936..>3262)
                     /locus_tag="VQU32_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2473438.1"
                     /translation="VKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMTSS
                     TNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLGLK
                     NKKEEN"
BASE COUNT          912 a          735 c          626 g          989 t
ORIGIN      
        1 ttttttacga aaatctattg aatatgccat aaaaggatta taccacattg tgtactattt
       61 ttggttcatt ttactatatc actttggact atatcggtct agttatgtct aaacgattat
      121 agctggatac taagagaaat caaaaaagca ctaagggaga acgtcccaaa gtgcttaata
      181 tcaaggcctc aaagcctgtc ttattcaata aaatattaca acatcttgtt gtagaattca
      241 acgacaagtg cttcgttgat ttctgggttg atttcgtcgc gttctggcaa gcgagtcaat
      301 gaaccttcca atttttcagc gtcgaatgat acgaatgctg gacgtccaag agtagcttct
      361 actgcttcaa ggattgctgg aactttcaat gatttttcac gaactgagat cacttgacct
      421 ggagttacgc ggtatgatgg gatatcaacg cgtttcccgt caacaaggat gtgaccgtgg
      481 tttacgaatt gacgagcttg acgacgagta gtcgcgagac caagacggta aacaacgtta
      541 tccaaacgac gttccaaaag aagcataaag ttgaaaccta ggattccgcc tttgattttt
      601 gtagcttgta cgaacaagtt acggaattgt ttttcaccta caccgtaagt gaaacgaagt
      661 ttttgttttt cagccaattg caaaccgtat tctgacaatt tagaacggtt gtttggtccg
      721 tgttgtcctg gtacgtagtt acgacgtgcc aattctttac ctgtacctgt aagtgaaagg
      781 ccaaggcgac gagcttgttt ccaagatggt cctgtataac gtgacatatg aatgtcctcc
      841 tgatataaat aatatttcgg tggaaatagt cacttagaaa gccctgattc gtgcagatgc
      901 ccttcgccta aacagccaag gttacttgtc ataagacacc tgttgacgag cttcatgctt
      961 tcctgctgat atttcacaca aaatccattt taccatgaat agctggattt gtaaaggggt
     1021 tttaggcttt attttcacta ccagagagat tgagaacgag ggtaaaggta cttcctagac
     1081 cgtactggct gctgactgtg atttccccac ccaattgatg ggccaattca cgcgcaatcg
     1141 caagtcctaa tccatgacca cctgtcttca tgttacgcga agtttcgaca cgataaaggc
     1201 gtttgaaaat attttccaaa tcctctgggg caatgccctg cccttcatcg gtcacactga
     1261 ttgaaagctg gcccttctcc agcttagtca ccacttccag cttggttcct ggagcagaat
     1321 atttaaaagc gttatcgacc agattcacca agatacgaga aagcttagca taatctccct
     1381 caatccgggc agactctggg attacctgca agtggacatc tcttctctcc tgctcaatca
     1441 aaaactgaaa ttcactcatg cactcaatta agagcttgtc cagaaaaata ctgtctttac
     1501 tggtagtttc cacctgattt ctagctgtgt ttagggtcaa aaaattcaac tcctcaacca
     1561 gtttattgag cctctccgtc tggcgtccaa tggttgctag ataatgagct tgctccgact
     1621 ccttgataat cccatccaaa atcccttcta ccgtcgcttg gatcgaagtg ataggggtct
     1681 taatatcatg cgacaactgg gcaatcatca agcccttttc tcgttcgctt tcttccaagg
     1741 aatcaaagct tacctgcaaa tcatgggaca tctcattaaa agtttgccct aattgctgaa
     1801 attctacagg accttgaacc tccaaatttg aaggaaaatc cttggccgct acccgcttgg
     1861 catgctcctt gagtttgccc aacgacgtaa agactggcaa taggagaaag agactaatcc
     1921 cagcaccgac aaggctggca acgatggtca tcccaagcaa aaagtaaatc tcgcctttcg
     1981 caatcagcat tttttgaaca gcccaaaaaa caaccaaaat ggttaagagg gttgaaataa
     2041 tatatccaac caaaatataa ctttttagtt tcatgtttgt cctctcggtt tctctatctt
     2101 atatcccaac ccccaaactg tcttaatagt gggcgtttgg tcactactat attttgccag
     2161 ctcctgtcga agagcatgga tatgcacatt caaggtattg gtgtcatcca cgtagtcttc
     2221 tttccagatc ttttcataga ggtctgtctt ggagaaaact cgctctggat tgctagctaa
     2281 aatccacagc aattcaaatg atttaacagt taaatccagc atttcttctc ctatttgaac
     2341 ttcatgacta ctatgattca tttttagatt gccaagggaa atcagctctg tttcgccccc
     2401 acgatgaagg cggcgcaaaa tattgtggac acgcaaaacc agctcacgtg ggctaaaagg
     2461 cttagcaata aaatcatctg ctcccaagct caggccgtaa atcttgtcct gttcactggt
     2521 cttagcagta ataaatagga aaggctgctc tggtgataag tattgaacct cactgattaa
     2581 atcataacca tccatccgag gcatcataac atctgtgata atcaaatcaa tcggtttttt
     2641 cttgaacagc tctagcgctt ccagtccatc atgggctacc aagacctgat aacctgcctg
     2701 aattaagtat ctctgatgaa tatctgtgat ttctacctcg tcgtcaacga gtaaaattgt
     2761 ctttcccatc tgtctctcct ttgataaaaa cagtgctata ctgctttaag tataacacta
     2821 tttttattgt tttacgatga ttttaatctg ctattgaaag aaagtcctga ttgctgattg
     2881 agttgttaaa ctaatcttag tcagcccact atccatcatt tatttcctta gctgattagt
     2941 tttcttcttt tttgtttttc aaacctagac caccgagtaa acctgcaagc gcaagcccaa
     3001 ggaaaccaat acttgccatt gatgtttgag tctcaccagt atttggtagc atagctttat
     3061 cctctgacat catcgtatca gacatcttgt tagcagaagc agccatgttt tcacctgcca
     3121 tcgtgttggt agaacttgtc atggtgtcag caggcatgct atctgtagta cctgtagcat
     3181 gattgtgatt catcggagtc acgccagaac cagagttaga aggtgataat gaaccatttg
     3241 ctgtgtctga tgtttcttta ac
//
LOCUS       DAVBBS010000098         2878 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVBBS010000098 DAVBBS010000000
VERSION     DAVBBS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.098"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(384..1859)
                     /locus_tag="VQU32_002054"
     CDS             complement(384..1859)
                     /locus_tag="VQU32_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET2473439.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            2566..>2878
                     /locus_tag="VQU32_002055"
     CDS             2566..>2878
                     /locus_tag="VQU32_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2473440.1"
                     /translation="MQDNYTTKGKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTI
                     HTKIKRRTVRKCLGKGRFKEVYSADYAQQSYENNRKHSVKRSSVTKELKEKILHYHNQ
                     KF"
BASE COUNT          904 a          624 c          519 g          831 t
ORIGIN      
        1 ataagaaaga cgctcatggc atcgctctta aacttctttc tgaccccaat cgtgaacaat
       61 ttcaacacga taatcggcaa gtagaactaa aaatactgac tagacatatt catcgtctca
      121 agaaaaaaca gtctgattgg aaagtacaat acactcgttg tcttgatatc atctttcctg
      181 agttggataa aatcgttgga aagcattcag aatataccta ccaactcttg acacgctacc
      241 ctaatcctca gaaaaggatt gaggcaggat ttgataagct gatagaaatt aagcgattga
      301 ccgcttctaa ttcaagggct ttttgccgtg ctctaaaatc atcgccaaat catcaaattg
      361 aaataagaaa tagtttagat aaactattga cttttcttat agtttgtctt tctcttaata
      421 acgttgatat gattgacctc tggatcttcc gtcccttgga gggagcaacc acgttccttg
      481 gcgaattgga tatagtcgac aatttctcgt gtaatgcgtt caccaggagc caagatagga
      541 atacctggag ggtaacacat aacaaattct ccacagacct gtccaacaga atcatccaaa
      601 gttaaacttt ttctttctga atagaaggct tcttgcggag acagcactaa ctcgggctga
      661 atatattctc ctgctatcaa atcttttcca tctcttgaat agagtctctt aatatcagcc
      721 agagcaccaa ccaagcgctc gatgtcttgg atgcggtcgc cgatggaaat ataggccaag
      781 atattgccga tatcaccaaa ctcgatctga atgtcgtatt cgtctcgcaa gaggtcataa
      841 acctcgatac ctgttaagcc aataccctga gtgtaaactg acaacttagt tacgtcaaaa
      901 tcgcaaaccg aaacaccgtc tattaactct tttgagtagg catagtagcc accgatagca
      961 ttgatttcat gacgagcgta ctcggatagc tcaatgactt cctcaaacga ctctttacca
     1021 cgaagggcca agttgcgacg tgaaatatcc aaactagcca tcaataagta agaggcagat
     1081 gtagactggg tcaggtttat aatctgacga acgtactcgg gattcatctg ctccccgatt
     1141 aaaagaagcg aactttgtgt caaactccca ccagacttat gcatggaaac tgctgccata
     1201 tcagctcctg catccatagc agaaattgga agtttaccag tgaaatgcaa atgcgctcca
     1261 tgggcttcat ctactaaaac catcatgcca gcttcatgag ccatttctgt caaccccttt
     1321 aggtctgaac agatgccgta gtaagtagga ttgttgatta ggatagcctt agcatctgga
     1381 tggtccttta tggcctgtgc tactcggtca ttttcaagac ctaaagcgat accaatctta
     1441 ggatctacac tcatctcgat atagatggga atggcaccac atagaaccaa cgcattgata
     1501 gcagatttat ggacatttcg tggcagaata atcttatctc ctgccttgca ggttgccaga
     1561 atcatagtct gcaccgatga agttgttcca ccaatcatta gaaaggcatg gctagctcca
     1621 aaagcatctg cagccagctc ctctgcatca cgaataatcg aaataggatg gcctaaatta
     1681 tccaagggtt tcatcgaatt gacatcaatg cctacacatt tttctcctaa gagttcgaca
     1741 agttctggat ttccccgtcc acgcttgtga cctggaacat caaagggaac aatccttttc
     1801 ttgcgtaact tcaccaaggc ctcataaatt ggggcttggt tttgatctaa ctctttcaaa
     1861 acatactcct ttcaatattt ttaggacctt atgaaaacta aggaactaaa ggttgactca
     1921 tgaaaaaaga gcaggttttc acctgctctg caatcttctg acgtgtttat tttggtttct
     1981 gttgagtatg tctgttcccg atgtttccta actctctagt agcaaatatt acgtacttta
     2041 ttgatcgttt cacaagatga cgtgtgcttt caccacacta aaaatttatg aattaggaca
     2101 agtgtcctaa catcaactta taaaagtagg cttttaaccc tatcaataat gtggcttttc
     2161 aaccacgctt cggcattcaa ccctgcctgt ccgtaggcat tttttactcg ggtttatatt
     2221 tgctagaaaa catcatctca ttttctaagc tttattacta tatcatgttt ttaagaactt
     2281 gtaaagtatt ttttgataaa aaacaaaaat ataatagatt gaaactagaa tagtacacct
     2341 ctgcttctaa aacattgtta gaaatagatt ttactgtcct gatcgatttg ttctgttctt
     2401 gtttcatttt actatatgtt cgtaaatgaa agttctataa tgaagttagc cacccagccc
     2461 tcctaaaaat tagatggttc aactagctga tagctcttgt aggcataaga aaacacctct
     2521 gtgttatact tgttgttcaa ccacaaacac aagaaaggca cagaaatgca agacaattat
     2581 actacaaaag gtaaacattt gacaatcgat agccgtcgct taatcgaaag atggaaaaaa
     2641 gaaggaaaat caaatagaga aattgcctct ctacttggaa aagctcctca aactatccac
     2701 actaaaatca agcgtaggac agtccgaaaa tgtcttggaa aagggcgctt caaagaggtt
     2761 tattctgccg actatgctca acagtcttat gaaaataatc gcaagcactc ggtcaagaga
     2821 tcaagcgtga ccaaggaact aaaggaaaag attctccact atcataacca aaaatttt
//
LOCUS       DAVBBS010000099         2841 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVBBS010000099 DAVBBS010000000
VERSION     DAVBBS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.099"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            368..1714
                     /gene="gdhA"
                     /locus_tag="VQU32_002056"
     CDS             368..1714
                     /gene="gdhA"
                     /locus_tag="VQU32_002056"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HET2473441.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
     gene            complement(2220..2390)
                     /locus_tag="VQU32_002057"
     CDS             complement(2220..2390)
                     /locus_tag="VQU32_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473442.1"
                     /translation="MKKENEYVILTIASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(2403..>2841)
                     /locus_tag="VQU32_002058"
     CDS             complement(2403..>2841)
                     /locus_tag="VQU32_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2473443.1"
                     /translation="SILDNITLKHEVTSQKIEEVCKAVQIYDEIMAMPMKFNTIISEM
                     GSNISGGQRQQIALARALINNPSIVILDEATSALDTINEERITKYIQSQGCTQIIIAH
                     RLSTIKDADIIFVMKGGKIVESGNHKYLITLGGEYYSLYTKRK"
BASE COUNT          899 a          529 c          479 g          934 t
ORIGIN      
        1 ttggatttct tctttagttg atttcatatc actattatat aatgattttt gattttagtc
       61 tggtataaat attgctttcc tccaaaatgg tcataatttt actagcaaat ctaacatatc
      121 acggataaat taacaagtga tttctgaatt gctaaacatt ttcttttctt atagcatacc
      181 atactttaag attttgtctt tgagaaagat atttccaaga aaaacgttcg tttttttatc
      241 tattttcaaa aaaaccaaaa actgaattga aagaattttt agaaaatttc tgttttttct
      301 ttacagtgaa aaaaaattct gctataatgt cttatgtaat aaaatatgac aataaaagga
      361 gaacgatatg acatctgcta aagaatatat ccaaagcgtg tttgaaactg tgaaagctcg
      421 taacgggcac gaggctgaat tcctccaagc tgttgaagaa tttttcaaca ctttagaacc
      481 tgtatttgaa aaacaccctg agtatatcga agaaaatatc ttggcacgta ttactgagcc
      541 tgagcgcgtg gtttctttcc gtgttccttg ggttgaccgt gatggaaaaa ttcaagtaaa
      601 ccgtggttac cgtgttcaat tcaactcagc tgttggacca tacaaaggtg gacttcgttt
      661 ccacccaact gtaaaccaag ggatcttgaa attcctcgga tttgaacaaa tctttaaaaa
      721 tgtcttgact ggacttccta tcggtggagg taaaggtgga tcagacttcg atcctaaagg
      781 taaaacagat gctgaagtga tgcgcttctg ccaaagcttc atgactgaat tgcaaaaaca
      841 catcggacca tcacttgacg tacctgctgg tgatatcggt gttggtggac gtgaaattgg
      901 ttacctttac ggtcaataca aacgtcttaa ccaatttgat gctggtgtct tgactggtaa
      961 acctcttgga tttggtggta gcttgattcg tccagaagca actggttacg gtttggttta
     1021 ctatactgaa gaaatgctca aagctaacgg taacagcttt gctggtaaga aagtcgttat
     1081 ttcaggttct ggtaacgttg ctcaatatgc tcttcaaaaa gcaactgaac tcggtgcaac
     1141 tgttatttct gtttctgact caaatggtta tgtcatcgat gaaaatggta tcgacttcga
     1201 tcttttggtt gatgttaaag aaaaacgtcg tgctcgtttg actgagtatg cagctgaaaa
     1261 agcaactgca acctaccacg aaggtactgt atggacttac gctggaaact atgacattgc
     1321 tcttccatgt gccactcaaa acgaaatcaa cggtgaagca gctaaacgtt tggttgctca
     1381 aggcgttatc tgtgtatctg aaggtgccaa catgccgagc gaccttgatg ccatcaaagt
     1441 ttacaaagaa aatggtatct tctacggacc tgcaaaagct gccaacgctg gtggtgtagc
     1501 cgtttcagct cttgaaatga gccaaaacag tcttcgcctc tcatggactc gtgaagaagt
     1561 tgatggccgt ctcaaagaca tcatgacaaa catcttcaac acagctaaaa cgacttcaga
     1621 aacatacggt cttgataaag actaccttgc aggagctaac attgctgcct ttgaaaatgt
     1681 agcaaatgcc atgattgcac aaggtattgt ttaagattta tttactcaat cctcaatcac
     1741 tacgattggg gatttttagc ttgaattaaa aaattggaaa atctaattaa ataaaatgaa
     1801 atgaaataaa aactgaacaa attgattgcg gaattcaaac caatttctaa catacttcta
     1861 gaaattaatt tgacttttct aatcgatttg ttcatatctt atttcaattt actatactta
     1921 cttaaccctt tttatactca atgaaaatca aagagcaaac taggaagcga gccgcaggct
     1981 gtacttgagt acggcaaggc aaagctgacg tggtttgaag agattttcaa agagtattaa
     2041 acaaaaagaa aaaccgctac tcctaagaat agcgatttaa tcatgttttt aagctactac
     2101 tgtagttgct ctattattta agtgttatga ataacttcaa tatctttaaa gctttttgtt
     2161 agatttataa tttcaactat agccatctct actccttagt ttacagcaaa aaattatgct
     2221 taattcttat tgtttttgta aacaatcagc gaacccaata ctattccatt caacaagcct
     2281 aaagaaatac catattcaac tggaaaatct aaaaaaattg caaacactat tccaatcatc
     2341 acccctagtg aggctattgt taaaattaca tattcatttt ctttcttcat tttctttaca
     2401 cctcatttcc tttttgtata taagctgtag tactctccac caagagttat taagtactta
     2461 tgatttcctg actcaacaat cttaccacct ttcattacaa aaataatatc cgcatcctta
     2521 atcgttgaca atctatgagc tataattatt tgagtacaac cctgactttg tatatacttt
     2581 gttattcttt cctcattaat agtgtctaat gcactagttg cttcatctaa aattacaata
     2641 ctaggattat ttattaatgc acgtgccagt gctatctgtt gcctttgccc acctgaaata
     2701 tttgacccca tctctgagat gatagtatta aatttcatcg gcatagccat gatttcatca
     2761 tagatttgaa ctgctttaca aacttcctct atcttttgtg aagtaacttc gtgctttaaa
     2821 gttatattat caagaatact t
//
LOCUS       DAVBBS010000100         2624 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVBBS010000100 DAVBBS010000000
VERSION     DAVBBS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.100"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            206..520
                     /locus_tag="VQU32_002059"
     CDS             206..520
                     /locus_tag="VQU32_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473444.1"
                     /translation="MWVLGFILFLIFFYSNDSKKIKKLEKKIKRLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            530..736
                     /locus_tag="VQU32_002060"
     CDS             530..736
                     /locus_tag="VQU32_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2473445.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            736..933
                     /locus_tag="VQU32_002061"
     CDS             736..933
                     /locus_tag="VQU32_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473446.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            989..1669
                     /locus_tag="VQU32_002062"
     CDS             989..1669
                     /locus_tag="VQU32_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HET2473447.1"
                     /translation="MGMFVGMFKARVESHEIILDVKALMPWISAICLLIGFISMFLTF
                     NFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAFNITGVVIPLAIFISLSEV
                     IILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLEFFATPKDVLNYINSYDEG
                     ERQANLEQSFRILFQLHQYVLPALYIFLIILSFLTGEIQLLAFLLVGAIHVYINVMQL
                     PMVKRYFK"
     gene            1681..2322
                     /locus_tag="VQU32_002063"
     CDS             1681..2322
                     /locus_tag="VQU32_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2473448.1"
                     /translation="MKLLKNLGWFLLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            2340..2570
                     /locus_tag="VQU32_002064"
     CDS             2340..2570
                     /locus_tag="VQU32_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473449.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
BASE COUNT          766 a          431 c          541 g          886 t
ORIGIN      
        1 atacctacgg caaggtgaag ctgacgtggt ttgaagagat tttagaagag tattaatgat
       61 agataaagtg gtcaggaacc tactcctgac ctttttcttt tgcaaaatga caaaaatcat
      121 aatttttttg acaactatac ttgtcaaaaa gaaaaagatg tgttacaatg taagcaagtt
      181 aagagaaaag aagaaaggag ctatgatgtg ggtactaggg tttatactat ttctgatttt
      241 cttttattct aatgattcta aaaaaatcaa gaaactagag aagaaaatca aaagacttga
      301 gcgaaaagag aaaggaaatg cagaaatgtc gagattatta caagaaatga ttggaaagga
      361 accaattata acgggagtgt atattgggcc agataactgg gaagttgtgg atgttgatga
      421 ggaatgggta aagctacgac gtgtagataa tacgggaaaa gaaaaattca agttgcaacg
      481 tattgaggat atccaaaccg ttgaatttga cggagagtag gtgtgtaaca tgcaactaaa
      541 aaatcgtcta aaagagcttc gagctcgcga tggtctcaat caaaccgacc tagccaaact
      601 ggcaggggtt tccagacaga ccattagcct actagaacgg gatgagtaca ccccatccat
      661 tatcattgcc ctgaaaatat ctcaaatttt caacgaaaca gtcgaatcgg tatttcgttt
      721 ggaggaggac gagtgatgaa caagtataaa gtgatttatt atgtagtggt catagcttta
      781 ttagtcagcg tatttctact gattgggatg gacctaagct ggtttaatcc ctatcaaagc
      841 gaccaatttg tttgggtcta ctttgctctc atcccagtaa ttgaatggat tgaaaagaaa
      901 tccaaaaatc tagcaagtga aaaaggagaa tgaatatgaa aaagaaacgt agattgttat
      961 ttttgatgtc tattgtcttg gggggttctt gggcatgttt gtagggatgt ttaaggcacg
     1021 tgtcgaatcc cacgaaatta ttttagatgt aaaagccttg atgccatgga tatcagctat
     1081 ttgtttactg ataggtttca ttagtatgtt tttgactttc aatttcttaa agaaaagcag
     1141 aaaatttcat tccttgtatc aagaggaaat ggatgacgat ctgaatgaaa cctattatgt
     1201 gcaaatgtat cggaatcttg agtttggaac cattgctttt aatattacag gtgtagtgat
     1261 tccattggct atttttattt cattaagtga ggtgattata ttgcatacaa accctcaaac
     1321 atttttcctt tctttcttac tctttgtggt attcttagtc gctcaaaaat ctctttttaa
     1381 aaccattgcg attgttcgtc agtttgattt ggaatttttc gctacaccaa aggatgtctt
     1441 gaactatata aattcttatg atgaagggga gcgtcaggct aatttggaac agagttttcg
     1501 aattttattc caattacacc aatatgtctt accagcctta tatatttttc ttattatcct
     1561 ttctttcttg acaggagaga ttcagttact agctttcttg cttgtaggag ccatccatgt
     1621 ttatatcaat gtgatgcagt tacctatggt aaaacgttat ttcaaataaa ggagttatct
     1681 atgaaattac ttaaaaacct tggctggttt cttctagccc ttctatcctt tttctttatc
     1741 tatggcttta ttcaggggct cgcgactgcg tcgcttgctt taggcgcttc accctatgct
     1801 gttactctac tctatgtggt cttggctgga gtatatgtgt acggcattta caaatggtat
     1861 cagaaagctc ctgtttatat tgagaagagc ggctttaacc gatttatttg gcttcctgcc
     1921 ttggtttggt tcttatctct ggtcgttcaa ttcttcttgc cagatgatcc ttcagtaaat
     1981 cagcaaatag cgacagactt gaccttgtct caaccacttt tctcattctt tgctgtggtt
     2041 atttttgctc ctttgacgga agagattgtt tttagaggga tgttagcacg ctatctcttt
     2101 cctaagcagg acaatagtaa acgaaccctg atttttcttc tggtatctag tcttctattt
     2161 gccttgattc attttccagg tgatgtgcaa caattttttg tctattttag ccttggtttt
     2221 agtttgggtt tggcttacat tagcagaaaa ggtctggtct acagtatttc tcttcacgct
     2281 ttgaataatt tagtcggctt tttgatgatt ctcatgctat aatagagtca ggaggtcaca
     2341 tgaaacgagt aattttatta gcagtgattc aggcagtcgt tctatttttc atcattggag
     2401 cgctagctta tgccttcaaa ggcgatttct tctataacta tctagcagtt gtctttgctc
     2461 ctattgcagg tgtactgcgt tttgggacgg cttacataac ggaaattgtc ttgcctcgaa
     2521 aggcagccga aatcgctgaa aagcgtaaag caggcaaaaa ttcaaaataa aagagtccgg
     2581 tgaagttcat cggatttttg tattatactc aatgaaaatc aaag
//
LOCUS       DAVBBS010000101         2607 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVBBS010000101 DAVBBS010000000
VERSION     DAVBBS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.101"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            114..1031
                     /locus_tag="VQU32_002065"
                     /pseudo
     CDS             114..1031
                     /locus_tag="VQU32_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822901.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            complement(1071..2015)
                     /gene="manA"
                     /locus_tag="VQU32_002066"
     CDS             complement(1071..2015)
                     /gene="manA"
                     /locus_tag="VQU32_002066"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HET2473450.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTILVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVESWTLEGQDLELIVSHP"
     gene            complement(2147..2428)
                     /locus_tag="VQU32_002067"
     CDS             complement(2147..2428)
                     /locus_tag="VQU32_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET2473451.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            2493..>2607
                     /locus_tag="VQU32_002068"
     CDS             2493..>2607
                     /locus_tag="VQU32_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET2473452.1"
                     /translation="MKSTKEEIQTIKTLFKDSRTAKYHKCLQIILFRLMGKS"
BASE COUNT          698 a          606 c          477 g          826 t
ORIGIN      
        1 aaaagtcgtg cgctgaacac caacaagacg cttaaatcgt gcatcagtta gttgtttact
       61 tgcttcatca ttcatagaac tactatgcca tattttgttt cgcaggaagt ctgatcattt
      121 caaatccagc cattgctcta ggagctcaag cgacctttat cctattgaat atcttcattg
      181 tatcacgtgg ggttcaaaaa gggattgaaa gagcttcgaa agtcatgatg cccctgctct
      241 ttatcatctt tgtcgtcatc atcgaacgct ctctcagttt gccaaatgcc atggaagggg
      301 ttctttactt cctcaaacca gatttttcaa aactgactag cgctggtctc ctctatgctc
      361 tgagacaatc tttctttgcc ctctcactag gggttacagc catgttgacc tatgcttctt
      421 acttggacaa gaaaaccaat ctggtccagt caagaatctc catcgtagcc atgaatatct
      481 cggtatccat catggcaggt ctagccattt tccaagctcg gtcccccttc agactgaata
      541 ttgaaggggg accgagcttg gtctttatcg tcttgcctca actctttgac aagatgcctt
      601 ttggaaccat tttctatgtc ctcttcctct ttgcgactgt cacttcttct gtcgttatgc
      661 tggaaatcaa tgtagacaat atcaccaatc aggacaacag caagcgtgct aaatggagtg
      721 ttattttagg aattttgacc tttgtctttg gcattccttc agccctatct tacggtgtca
      781 tggcggatgt tcacattttt ggtaaagcct tctttgacgc tatggacttc ttggtttcca
      841 atctcctcat gccatttgga gctctcttcc tttcactttt tacaggctat atctttaaaa
      901 aggctcttgc aatggaggaa ctccatctcg atgaaagagc atggaaacaa ggactgttcc
      961 aagtctggct cttccttctt cgtttcgtca ttccaatcat catcgtggtc ttcatcgccc
     1021 aatttatgta atcaaaagga cttgagtaat gaactcaggt cctttctttt ttatggatga
     1081 ctaacaatta attctaaatc ttgcccttct agagtccaag attcaacatc acttggtagg
     1141 ataaagtgtc tccctttttg gattggatag tttttcccgt caacagttag ttgaccttga
     1201 ccagccaaga cactcaacaa gctgtagtca gctgtctttt caaagttaac ttttccagta
     1261 atttcccatt tgtaaactgc gaagaaatca ttggatacaa ggatagtgga acgtagatca
     1321 tctgctttga ttgtcactgg acggctattt gcgggttcac caatgttcaa gacatcgata
     1381 gatttttcaa ggtgaagttc acgtaaattg cccttgtcat ccttacggtc aaagtcatag
     1441 acacggtaag tggtatcgct agactgctgg gtttcaagaa tcaagatacc cgccccgata
     1501 gcgtgcatgg taccgcttgg tacatagaag aaatctcctg ctttaactgg cacttttgtc
     1561 aacagcgcat cccagttctt atcctcgatt tgctggcgga gttcttcttt tgacttggca
     1621 ttgtgaccgt agataatctc tgaaccttca tctgctgcaa tgatgtacca acattctgtt
     1681 tttccaagtt cgccttcatg ctcgagtcca taagcatcgt ctgggtgaac ttggacactg
     1741 agccagtcgt tggcatcgag gatcttggtc aaaagtggaa atacaggttc tggacgattg
     1801 ccaaataatt cacggtgttc cgcatacaaa gtagcaagat ctgttccctc gtaacgacca
     1861 ttggcaactt tagaaacacc atttagatga gctgagatag cccagtactc tccgactttt
     1921 tcacttggga tgtcgtagcc aaactcatca cgtagcttgg taccacccca gattttttct
     1981 tgcataactg attgtaaaaa taatggttct gacatcttaa tctcctgtct gatttttctc
     2041 ccctcattat agcaaaaaaa gagttccatt tgaactcttt tttacatctt ataaagcagg
     2101 gagaagattt tataaaaata gtaaacaaat gtgctctact cgatgcttac accattgcta
     2161 taaatgacat gtaccaatag aaggacttct tcttgctacg tttgagagtc ccatttccta
     2221 cattatctcg atctacatag ataaagccat agcgcttatt catttcccct gtgccagctg
     2281 aaaccgaatc gatacagccc catgtcgtat aaccaagcaa gtcaaccccg tctttgtaaa
     2341 tggcatctcg catggccttg atgtgggcct ctaagtaagt aatccgatag tcatctgcta
     2401 cataaccatt ctcatccggt gtatccatag caccgagtcc attttctacg ataatactat
     2461 actaaaatca aaaagcatta tataatagtg atatgaaatc aactaaagaa gaaatccaaa
     2521 ccatcaaaac actttttaaa gactctcgta cagctaaata tcataaatgc cttcaaatca
     2581 ttctatttcg tctgatgggc aaatctt
//
LOCUS       DAVBBS010000102         2135 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVBBS010000102 DAVBBS010000000
VERSION     DAVBBS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.102"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(88..292)
                     /locus_tag="VQU32_002069"
                     /pseudo
     CDS             complement(88..292)
                     /locus_tag="VQU32_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            649..>2135
                     /gene="pcpA"
                     /locus_tag="VQU32_002070"
     CDS             649..>2135
                     /gene="pcpA"
                     /locus_tag="VQU32_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HET2473453.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDGA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGVVGWVKDKGLWYYLNESGSKATGWVKDKGLWYYL"
BASE COUNT          769 a          298 c          348 g          720 t
ORIGIN      
        1 gtgtttaagt tgataagctg tttttaacac agctaacatc tcttcaaaag tggtgcgctg
       61 aacaccaaca agacgcttaa atcgtgcatc ggttagttgt ttacttgctt cataatttcg
      121 cagggagtct attgactctt tggtaggtgt caatgttttt ttcatctatc ccgagaatta
      181 ttttcccgcc atttgtattt gcaaatgctg agtaggtttc ccagaaagac tctggaagat
      241 tgtttttagc ttttttgtat tctaaatcaa ccccttcaaa ttttaagtcc atatttttcc
      301 tttacatctg ttttttgtgg ttctggtatt tgttcaagtt gagtgataat atagcgaatt
      361 gaatttcgag agtttttact cagttaattt cttttttaac ccactttaat ttctttttta
      421 acccacttta atttcttttt taacacgggt taaaaaagaa attaaagtgg gttaattttt
      481 cttgacttaa aatttaaaaa tgatatactg tgtttatgga gagatgacaa tttataaaat
      541 gtactactct attctactag ataatgattg aataaaactt tttataacaa cggctagcaa
      601 agttataatt ttatatctat tagaaaataa atatgtaagg aaatttaaat gaaaaaaact
      661 acaatattat cattaactac agctgcggtt attttagcag catatgtccc taatgaacca
      721 atcctagcag atactcctag ttcggaagta atcaaagaga ctaaagttgg aagtattatt
      781 caacaaaata atatcaaata taaggttcta actgtagaag gtaacatagg aactgttcaa
      841 gtgggtaatg gagttactcc tgtagagttt gaagctggtc aagatggaaa accattcacg
      901 attcctacaa aaatcacagt aggtgataaa gtatttaccg ttactgaagt agctagtcaa
      961 gcttttagtt attatccaga tgaaacaggt agaattgtct actatcctag ctctattact
     1021 atcccatcaa gcataaaaaa aatacaaaaa aaaggcttcc atggaagtaa agctaaaact
     1081 attatttttg acaaaggcag tcagctggag aaaattgaag atggagcttt tgatttttct
     1141 gaattagaag agattgaatt gcctgcatct ctagaatata ttggaacaag tgcattttct
     1201 tttagtcaaa aattgaaaaa gctaaccttt tcctcaagtt caaaattaga attaatatca
     1261 catgaggctt ttgctaattt atcaaattta gagaaactaa cattaccaaa atcggttaaa
     1321 acattaggaa gtaatctatt tagactcact actagcttaa aacatgttga tgttgaagaa
     1381 ggaaatgaat cgtttgcctc agttgatggt gttttgtttt caaaagataa aacccaatta
     1441 atttattatc caagtcaaaa aaatgacgaa agttataaaa cgcctaagga gacaaaagaa
     1501 cttgcatcat attcgtttaa taaaaattct tacttgaaaa aactcgaatt gaatgaaggt
     1561 ttagaaaaaa tcggtacttt tgcatttgca gatgcgatta aacttgaaga aattagctta
     1621 ccaaatagtt tagaaactat tgaacgttta gccttttacg gtaatttaga attaaaagaa
     1681 cttatattac cagataatgt taaaaatttt ggtaaacacg ttatgaacgg tttaccaaaa
     1741 ttaaaaagtt taacaattgg taataatatc aactcattgc cgtccttctt cctaagtggc
     1801 gtcttagatt cattaaagga aattcatatt aagaataaaa gtacagagtt ttctgtgaaa
     1861 aaagatacat ttgcaattcc tgaaactgtt aagttctatg taacatcaga acatataaaa
     1921 gatgttctta aatcaaattt atctactagt aatgatatca ttgttgaaaa agtagataat
     1981 ataaaacaag aaactgatgt agctaaacct aaaaagaatt ctaatcaggg agtagttggt
     2041 tgggttaaag acaaaggttt atggtattac ttaaatgaat caggttcaaa ggctactggt
     2101 tgggttaaag acaaaggctt atggtattac ttaaa
//
LOCUS       DAVBBS010000103         2039 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVBBS010000103 DAVBBS010000000
VERSION     DAVBBS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.103"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..105
                     /locus_tag="VQU32_002071"
     CDS             <1..105
                     /locus_tag="VQU32_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2473454.1"
                     /translation="AFLREIAAHFDCAVPSVWAALKQIKVTLKKDDDL"
     gene            92..625
                     /locus_tag="VQU32_002072"
     CDS             92..625
                     /locus_tag="VQU32_002072"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2473455.1"
                     /translation="MTIFKEQESEKVAEFLDILDNLKDLPIVYIDETGINRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLL
                     REVPTIFECLECFFKTR"
     gene            983..1237
                     /locus_tag="VQU32_002073"
     CDS             983..1237
                     /locus_tag="VQU32_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473456.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQV
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            1348..>2039
                     /locus_tag="VQU32_002074"
     CDS             1348..>2039
                     /locus_tag="VQU32_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HET2473457.1"
                     /translation="MNIILIAKPLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSYRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDG"
BASE COUNT          745 a          336 c          415 g          543 t
ORIGIN      
        1 gcttttttac gggaaattgc ggcacatttt gattgtgctg ttccttcagt atgggcagct
       61 ttaaagcaga ttaaggtcac tttaaaaaaa gatgacgatc tttaaggaac aagagtcaga
      121 gaaagtcgct gagtttcttg atattttgga taacctaaaa gatttaccaa tcgtatatat
      181 tgatgaaaca ggaatcaacc gctacctcta tcgtccttat gcaggggctc ctagagggga
      241 gaaagtctat gaaaagatta gcggacgtcg ttttgagcga acttcaattg ttgcaggaca
      301 agtagacgga gagtttatag ctcccatgat ttacaagaaa agcatgacaa gcgatttctt
      361 tgtggagtgg ttcaaaacgc aactcctacc tgctttgaag acacctcatg ttattgtcat
      421 gggcaatgct agttttcatc ccaagaacat tttggatgaa ctctgcatcc aagataaaca
      481 ctttttctta cctctaccac cttattcacc ggatttgaat cctattgagc aagcttgggc
      541 tatcttgaaa aagaaagtga cggatttatt aagggaagtt ccaactattt ttgaatgttt
      601 ggaatgcttt tttaaaacta gatgactata acggttctaa aggaacctat cgagtagtca
      661 ttaaaactaa ggatactgct ggttaagaga agacggtata cgatcaaacc attcaccgtg
      721 tagccgaaat cgttcagaat gaagacttgt atcagaatga agacttgtat aagaaaggtt
      781 tgaatgttga acttgcgcac caacaaatta agggattttt tgaagcagag tttaaaaatc
      841 gtattaatgg agttcttaat actaaaataa aaaatagtac attaaatcgt gtaaataaaa
      901 aaactataca ccagagcaac aaaaactcca tgatcaattt gaagcagaag caacggaaga
      961 tgctaaaaaa caaggcgata ttgtgttgaa tgttgaccag gatttcatga gcatatctaa
     1021 gtctaataaa agtggttcag actggaagaa aactttcaca gtgaggataa ccaataggct
     1081 agcaaatgac ttgaataatg tcttgaaaca ggttgataaa gatactccta ataccccaac
     1141 ttggctaaac tcagctgctt ctaaagctaa agatgatgac agagtatata aactactgaa
     1201 gactcttata ccaggagaaa attacctatc atgttaagga taatcagcta gaagtagaaa
     1261 cagataaata cacatatact gccgctagaa atggtagtaa ggaagttggt attcaagagt
     1321 cagatatagc agcaactcta agtgccgatg aatataattc taatcgccaa acctttgaga
     1381 gagaatacaa atacaaaagc aaatgccctt aataatggtt gggctagatc tggttctgaa
     1441 gagttcaaaa agttctccca ctttgtaggg gtagacaaag ggattgtgcg aacgaatgta
     1501 ctgactggta aaaaactatc tgataaaatt aggaaagaag tgggctctgg agatagcaaa
     1561 ctaggaaaag gcggctattt ctctactggg gatgttctat taggaaaaga tgttgtttct
     1621 tataccgtac aagtattttc agagaataat gaaagagtag gagtaaacac tcaaagttac
     1681 cgtgttcagt ataatctccc aattctagct gacttttcag tcatccaaga tactgtggaa
     1741 ccatcacgaa ccgttgttga aaaaatcatt ccaaaactaa atattcccga agaagagaaa
     1801 gggaaaataa ccgaagaaat caagaaaaag aaaaaaacct cagaattggc agaactaatc
     1861 tcagaaaatg tgaaagttcg ctatgttgat gaacaagggc gtttgctatc attgaaaaat
     1921 gatactggaa ttggagaaaa agaaagtgac ggaacctaca ttaccaataa aaaacaactg
     1981 attggtacca gctacaatgt cacagataaa aaactcagta gcatgactac tactgacgg
//
LOCUS       DAVBBS010000104         1822 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVBBS010000104 DAVBBS010000000
VERSION     DAVBBS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.104"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..317)
                     /locus_tag="VQU32_002075"
     CDS             complement(<1..317)
                     /locus_tag="VQU32_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473458.1"
                     /translation="MILSKVRCYIYNSKIRGKVLYELYEFINENKQLMDAINSIKVDS
                     SNGFSIQILHLGEQQTTVLLTDFFTKTLDLFKEFRVTNKSTLEQVKNQISILNSSGPK
                     MDLT"
     gene            complement(330..1349)
                     /locus_tag="VQU32_002076"
     CDS             complement(330..1349)
                     /locus_tag="VQU32_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic biosynthesis protein"
                     /protein_id="HET2473459.1"
                     /translation="MSNTLKQLLLFREEFIKNILNGIDVDETVVIENDFVEELENFLK
                     ENDLLDFSNSIFFQLSGDKVIVNNIYPGYLAYFNRFLTFYPKIFAIKEVGEYIDFLNF
                     EKRIADMFYCWGFNANSRVLTAEKIIEIPNHQINAKYIKKVIKMSELLFGVDSNNRIR
                     LLNNRRELIKTIFQGSVIKSAIPALAATLDTISNSGSFQISLSKQIINNLVLSGKDKV
                     TIPRIELDNVVLSRQKIIVSVSYINDFLDLRFPDKIEYILNFLEGSGFNTKVMVYFGK
                     IVEEDKNYFVNLEKPQYFDFTSILFNKLFFKELNKFDYMVIEELIPNLDEDVMMTEYI
                     KEYTE"
BASE COUNT          626 a          318 c          190 g          688 t
ORIGIN      
        1 gttaaatcca tttttggacc agaagaattt agtatgctaa tttgattttt cacctgctca
       61 agagtactct tatttgtaac cctaaattcc ttaaacaagt ctaaagtttt agtaaaaaaa
      121 tctgttaata agacagttgt ttgctgttct cctaaatgta atatttgaat actaaaacca
      181 ttactactgt caactttaat tgaattaatt gcgtccatca attgcttatt ttcatttata
      241 aattcatata attcatacag aactttacct ctaattttag aattatatat gtaacaacgt
      301 actttactta aaatcataat agattgctcc tattcagtat actcttttat atactctgtc
      361 atcattacat cttcatcaag atttggaatg agttcttcaa taaccatgta atcaaacttg
      421 tttaattctt taaagaataa cttattgaaa agtatactgg taaaatcaaa atactgaggt
      481 ttttcaagat taacaaaata gttcttatct tcttctacaa ttttcccaaa gtagaccatt
      541 actttagtat tgaaaccaga tccttctaaa aaatttaaaa tgtattctat tttatctgga
      601 aatctcaaat ccaaaaaatc atttatatac gaaactgata caataatctt ctgtcttgaa
      661 agaacaacat tatctaactc tatcctagga attgtaactt tatctttacc acttaatact
      721 aaattattaa ttatctgctt tgataaagaa atttgaaatg atccactgtt actgattgta
      781 tccaaagtag cagctagagc aggaattgct gacttaatta cagaaccttg gaaaatagtt
      841 tttatcaatt cccttctatt gttaagcaac cgaattctgt tattactatc aacaccaaac
      901 aagagttcac tcatttttat aacttttttt atgtacttag cattgatttg gtgatttggg
      961 atttcaatta ttttttcagc tgtcaacact ctagaatttg cattaaatcc ccaacaataa
     1021 aacatatccg caattctctt ttcaaaattt aaaaagtcaa tatattctcc aacttcttta
     1081 atagcgaata ttttcggata aaatgtaagg aacctattaa aatatgctaa atatcctgga
     1141 taaatattat tcactattac tttatctcca gataactgaa aaaatatgct atttgaaaaa
     1201 tctagcaaat cattctcttt taaaaagttc tccaactctt ctacaaaatc attttctatt
     1261 actactgttt catccacatc aatcccattc aaaatatttt ttatgaactc ctctctaaaa
     1321 agtagtaatt gcttcaatgt atttgacata tattcatctg gataatatgt ttccaaatta
     1381 acattagcga gtctaggagc ttctttgatg attatgttaa ctatatcctg aactatttct
     1441 tctgatgtcc ctaccttctg gtgttctctc ctgtgtttat tataataaca ttgtccgaat
     1501 agtagctgaa aaaacaaatt actatcaaat aatctcgtta gaattaatag ctttcttatt
     1561 gacttattat caacttttaa actttctttt tcaatttgcc tcaactctac aatgtcttga
     1621 taaataaaat tatgttctat aaaaatttac ttcaactagc tatggtttaa tagatttttt
     1681 aaaggggata attgtctaaa atttaacttt ttacaaatca agatgtctct ataaactagc
     1741 aacctctcta ataactttac ccctatagct atagacaatc gagtagggga taatctctag
     1801 cccctctcac accaccgtac gt
//
LOCUS       DAVBBS010000105         1671 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVBBS010000105 DAVBBS010000000
VERSION     DAVBBS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.105"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            100..1575
                     /locus_tag="VQU32_002077"
     CDS             100..1575
                     /locus_tag="VQU32_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HET2473460.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNIVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
BASE COUNT          474 a          335 c          334 g          528 t
ORIGIN      
        1 tccagtattt gacaatattg aagcagctat taaagaacac ggattaagct aattctaatt
       61 tgttaacatt catataacat aaaaaaggga ggaactgtta tggatgcaat ctttgaccta
      121 atcggaaagg ttttcaatcc cattttagaa atgggtggac ctgtcatcat gttaatcatt
      181 ttgacagtat tggctttact ttttggagtg aaattctcca aagcgcttga aggtggtatc
      241 aaacttgcca tcgctcttac aggtatcggt gctatcatcg gtatgctaaa tggtgctttc
      301 tcagcatcac ttgcaaaatt cgttgaaaac actggtatcc aattgagtat taccgacgtt
      361 ggttgggcac cactcgcaac aatcacttgg ggttctgctt ggacactata cttcttgctc
      421 atcatgttga ttgtcaacat agtgatgcta gctatgaaga aaacagatac acttgacgtt
      481 gatatctttg atatctggca cttgtctatc acaggtctct tgattaaatg gtatgctgat
      541 aacaatggtg tgagtcaagg ggtttcactc tttattgcta cagcagctat cgtccttgtc
      601 ggtgtgttga aaattatcaa ctctgacttg atgaaaccta catttgatga ccttcttaac
      661 gccccaagtt catcaccaat gacatcaact cacatgaact acatgatgaa cccagttatc
      721 atggttttgg ataagatttt tgaaaaattc ttcccaggcc ttgataaata tgactttgat
      781 gctgctaaat tgaacaagaa aatcggtttc tggggatcta aattcttcat cggtttcatc
      841 cttggtatcg ttatcggtat tatgggaact ccacatccaa ttgcaggtgt tgcagatgca
      901 gataaatggc gtcttgttat caaaggatgg ttgtctcttg gtttgactgc cggtgtatct
      961 ttggaactct tctcacttat cggttcatgg ttcatcgcag ccgtagaacc actatcacaa
     1021 ggtattacaa acgttgctac taaacgtctt caaggacgta aattcaatat cggtcttgac
     1081 tggccattca tcgctggtcg tgctgaaatc tgggcttgtg ccaacgtact tgcaccaatc
     1141 atgttgattg aagcagtgct tctttcaaaa gttggaaatg gtatcttgcc acttgcaggt
     1201 atcatcgcta tgggtgttac tccagctctc ttggttgtaa ctcgtggtaa attgctccgt
     1261 atgattatct tcggaacact cttgttgcca ctcttccttc tttcaggtac acttattgca
     1321 ccatttgcaa cagaacttgc taaaggtgta ggtgccttcc cagaaggtgt gagccaaact
     1381 caattgatta ctcactctac tcttgaagga ccaatcgaaa aacttcttgg ttggacaatt
     1441 ggtaacacta caactggtga tatcaaagca atccttggtg cagtagtctt ccttgtattc
     1501 tatatcggta tctttgcttg gtacagaaaa caaatgatca aacgtaacga agagtacgca
     1561 gcaaaagcaa aataatgcgc tcctataaaa tataaactca atgaaaatca aagagcaaac
     1621 taggaaacta gccacaggtt gctcaaaaca cagttttgag gttgtagata a
//
LOCUS       DAVBBS010000106         1638 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVBBS010000106 DAVBBS010000000
VERSION     DAVBBS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.106"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            108..407
                     /locus_tag="VQU32_002078"
     CDS             108..407
                     /locus_tag="VQU32_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic biosynthesis protein"
                     /protein_id="HET2473461.1"
                     /translation="MDSYAINFWESKGVGIDKKILEFIQKNITVIKNKNFLNKDFDIY
                     VKKIHNLFFVISTLVSKEQYMGIVSSQIHMFANRMGISPTIEVTMLDFFLREDSD"
     gene            409..1179
                     /locus_tag="VQU32_002079"
     CDS             409..1179
                     /locus_tag="VQU32_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HET2473462.1"
                     /translation="MEKYFFDISIFEKIKKVLSYQSKYWEISKPRDKYSFKSQNRISG
                     SNSIKFKDFELDYELESLGKILLKRRSFLLNEFDKNASFDKLIYLLKTVFSPTNCKFV
                     FGEEVPLFSYPTSGALNSITPFVYIDNMDEVKNGIYEVKYDGLEFKKSMNIIDLETFT
                     PITSKNSDVQNNSFKKVNMIIMLCSNYENIFNKYGLLAYRLLHLEAGHIAQNIQLVST
                     ALGIKSLPMGGWFDDKIETFINEISDKKNIYLYAIAVG"
     gene            1199..1363
                     /locus_tag="VQU32_002080"
     CDS             1199..1363
                     /locus_tag="VQU32_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473463.1"
                     /translation="MNAEDKFRELENENKLLKLHNKELENEIQNGWKFWIWVTVPILG
                     LIYAIIQKIF"
BASE COUNT          601 a          180 c          267 g          590 t
ORIGIN      
        1 ataactatta ttacgaaaaa aaattcttgt gtaaaattct attgactttc tatattattt
       61 ttgtatttca taacgaatgt aatttaacaa gtgaagaaat tttaaagatg gattcctatg
      121 ctattaattt ctgggagagt aaaggggtcg gtattgacaa aaaaatcttg gaatttattc
      181 aaaaaaatat tacagttatt aaaaataaaa attttttgaa taaagatttt gatatttatg
      241 ttaaaaagat tcataattta ttctttgtca tttctacttt agtttcaaaa gagcaataca
      301 tggggatagt ttcttcccaa atacatatgt tcgcaaatag gatgggaatt tctcccacca
      361 ttgaagttac catgctagat ttttttctga gggaggattc tgattaatat ggaaaaatat
      421 ttttttgata tttctatttt tgaaaaaata aaaaaagtat tatcatacca atctaaatat
      481 tgggaaatat cgaagcctag ggataagtac agtttcaaaa gtcaaaatag aatatctggc
      541 tctaatagca ttaaatttaa agattttgag ttagattatg aactggagtc acttggtaaa
      601 atacttttaa aaaggagatc gtttttacta aatgaatttg ataagaatgc gagttttgac
      661 aaactgattt acttgttgaa aactgtattc tctcctacaa attgcaagtt tgtatttgga
      721 gaagaggttc ctttatttag ctatcctaca tcaggtgcgt taaattcaat tactcctttt
      781 gtttatattg ataatatgga tgaagtaaag aatggtatat atgaagtaaa gtatgacgga
      841 cttgaattta aaaaaagtat gaatattata gacttagaaa catttacccc aataacaagt
      901 aaaaacagtg acgtacaaaa taattcattt aaaaaggtaa atatgataat tatgttatgt
      961 tcgaattatg aaaatatttt caataaatat ggattattag cttatcgact tttgcattta
     1021 gaggctggtc atattgcaca aaatatccag cttgtttcta ctgcgctagg aataaaaagt
     1081 ttaccgatgg gaggttggtt tgatgataaa atagaaactt ttattaatga aattagtgat
     1141 aaaaaaaata tttatctcta tgcaattgca gttggataaa tttaaggagg aagtgtagat
     1201 gaacgcagag gataaatttc gcgaactaga aaatgaaaat aaacttctaa aacttcataa
     1261 caaggagctt gagaatgaaa ttcaaaatgg atggaaattt tggatatggg taactgtccc
     1321 tattttaggt ttgatatatg ctattattca aaaaattttt taattatgct agtttgtcag
     1381 gttgagtagt ttttgttcaa aatttaataa acttttttca atgaagatta tctaaagatt
     1441 gcttctcaga tgagaatggc ttctctatct tatataaatt atcttcatag agtgtcctgt
     1501 tattcatgtt atcacagaat agatagtttt tttggctcta taatatttgt agtgggtaaa
     1561 tcccctatag atattatgga gcctattttt gtgtagaaaa aaagtcccat atcatctata
     1621 atgaaaagca accaaact
//
LOCUS       DAVBBS010000107         1455 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVBBS010000107 DAVBBS010000000
VERSION     DAVBBS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.107"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(412..804)
                     /gene="rpsI"
                     /locus_tag="VQU32_002081"
     CDS             complement(412..804)
                     /gene="rpsI"
                     /locus_tag="VQU32_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET2473464.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(824..1270)
                     /gene="rplM"
                     /locus_tag="VQU32_002082"
     CDS             complement(824..1270)
                     /gene="rplM"
                     /locus_tag="VQU32_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET2473465.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
BASE COUNT          420 a          290 c          279 g          466 t
ORIGIN      
        1 cccaaactct tcacacaaga gttctaactt acccattcta tggaatcttg cattatccat
       61 aatatagtag attgaaacta gaatagtaca cctctacttc taaaacattg ttagaattcg
      121 atttgactat cctcggtttg tcctattctt atttcacctt actataaaaa taaggtaaac
      181 ttttcaaaaa aacacgaaaa aagcacccat agggcaaacc tttgagtgct ttgaaacatt
      241 gatataattg tattgattag cgttttgagg caactgacct tgtcaggttg cagccacatt
      301 tgtaagcaac taaagtcgca acaactgtgt caattgcacc aatttagtaa gaaagtataa
      361 aaaaagaaca ccccgaaagg tgctctgtat aagtatagta attctttcga attaacgttt
      421 actaaattgt gatgctttac gagctttctt aagacctggt ttcttacgtt caactttacg
      481 tgagtcacgt gtaagaagtc ctgcgcgttt caatgaatcg cggaagtctg ggtctacttg
      541 aagaagggca cgagcgatac cgtgacggat agctcctgat tgaccagcgt atccaccacc
      601 tacaacgtta acgaaaacgt cgtatgaacc tacagttgaa gtaactgcga atggttggtt
      661 gatgacaaga cgaaggtcag catgtgggat gtactcttca acatcttttt tgttaacagt
      721 gattttacca gttcctggaa caaggcgaac gcgtgcaaca gcgtttttac gacgtccagt
      781 acctgcatat tgtgcttgtg acatacttta tttgttcctt tccttagata agtcctgaaa
      841 tgtcaagaac ttctggttgt tgtgcagcat gagtgtgctc agctccaaca aatactttca
      901 acttcatacc ttgagcgcgt ccaagtgtat tgtgtggaag cataccttta actgatttct
      961 caatcaaacg tactgcattt ttagaacgaa gttcacctgc agagatttgt ttcaatccac
     1021 ctgggtggtt tgagtgagtg tagtagattt tatcagttgc ttttttacca gtcaatttaa
     1081 ctttttcagc attgataaca atcacgaagt cacctgtatc agtgtgtggt gtaaatgttg
     1141 gtttgttttt tccgcgaagt acgctagcaa ctactgcaga aagacgtcca agtggtacat
     1201 cagttgcgtc aactacgtac catttacgtt caacttggcc tggtttagcc ataaatgttg
     1261 ttttgttcat gatttctcct atatatagtt cgatttttgt ttacggtgat gggtgttcct
     1321 tcccatcgaa aagtatttgg aaggttccgg ggcctttcaa atggggtaaa caataccgcc
     1381 tactatcata ccaaatttca cccctaaaag tcaatagata tgaaaaatat ttttcggtaa
     1441 cttctcaaag taact
//
LOCUS       DAVBBS010000108         1389 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVBBS010000108 DAVBBS010000000
VERSION     DAVBBS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.108"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(704..1389)
                     /locus_tag="VQU32_002083"
                     /pseudo
     CDS             complement(704..1389)
                     /locus_tag="VQU32_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          386 a          283 c          287 g          433 t
ORIGIN      
        1 tacgtgacag agtccagttc tatcagaccg ttcgttttac agacacaagt gaagtaggat
       61 tacttcgatt agttgtttac cccttcgctc cacttccatt acagaagttt cctcactact
      121 atgggctcgg ctgacttctc atgattcgtt gttactacgc tttcggcgct catgagacct
      181 cacgggataa gccgtacatc tttcctcgtt tatacaagat atgaggacgt taaaaggtta
      241 caatgaaaat aggaaatctg acgcagagtc tttagactct aggaagattt atctttttca
      301 catagacctt agtccgaatt caattactct cgtgatttac gcatataggt tacgactacc
      361 tttttggact ttagagtttt aagccccctt atccgctgta tacgccttac tatcacgttc
      421 ctgttcgtag agcctcgatt tcgctatccc ttcctctgac gattcttatt tcctgcgtca
      481 gacttaaaac ggtctatccc cagaccgttt taatccgcta cctcacgata gtcaggcttg
      541 ggaatcgcta ttgggttcac cggtagcagg tgcccacgga ggacttacac ctcagatgca
      601 cgacatgccc gtcgtactta aaatcagcct aaatgattca ggctgatttt tctattgatt
      661 caaaaaaacg gctctttgtc aactgtagtg ggttgaagaa aagctaagat cgagaaagga
      721 caaatttcgt cctttctttt ttgatattca gagcgataaa aatccgtttt ttgaagtttt
      781 caaagttccg aaaaccaaag gcattgtgct tgataagttt gatgagatta ttggtcgctt
      841 ccaatttggc gtttgaatag ggtagttgaa gggcgttgat aatcttttct ttatctttga
      901 ggaaggtttt aaagacagtc tgaaaaatag gatgaacctg cttcagattg tcctcaataa
      961 gtccgaaaaa tttctctggt tccttattct ggaagtgaaa aagcaagagc tgatagagct
     1021 gatagtggtg tttcaagtct tccgaatagc tcaaaagctt gtctaaaatc tctttattgg
     1081 ttaaatgcat acgaaaagta ggacgataaa aacgcttatc actcagttta cggctatcct
     1141 gttgtatgag cttccagtag cgcttgatag ccttgtattc atgggatttt cgatgaaact
     1201 gattcatgat ttaaacacgc acacgactca tagcacggct aagatgttgc acaatgtgaa
     1261 agcgatcaag aacgatttta gcattcggga gtgaaacagt ctgggagact gtttcagcct
     1321 gagcctagaa atttgaaagc gaagctgtct agctaagtca tagtaagggc taaacatatc
     1381 catagtaat
//
LOCUS       DAVBBS010000109         1363 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVBBS010000109 DAVBBS010000000
VERSION     DAVBBS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.109"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            84..491
                     /locus_tag="VQU32_002084"
     CDS             84..491
                     /locus_tag="VQU32_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2473466.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATSFKEVQKPLENFN
                     HIAWFPIDEAIQNLKRGSHKWAIESWKKQHKIG"
     gene            656..955
                     /locus_tag="VQU32_002085"
     CDS             656..955
                     /locus_tag="VQU32_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HET2473467.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVNEANIKTLYSEKLIGISIPIK"
     gene            1216..>1363
                     /locus_tag="VQU32_002086"
     CDS             1216..>1363
                     /locus_tag="VQU32_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET2473468.1"
                     /translation="MMIVLSDLGQVHLVCGKTDMRQGIDSLAYVVKTHFELDPFSGQV
                     FLFCG"
BASE COUNT          473 a          268 c          237 g          385 t
ORIGIN      
        1 actataataa taacgatact agataatgag gaaaatgcaa tggcaaaccc aactttcgga
       61 gaaaaaaagc gaatacagac tatattgctc gctatggcgt atatgcagtt atccctaatc
      121 ctgaacaaaa acaaattgtt cttgttcaag aaccaaatgg tgcttggttc ctaccatgtg
      181 gagaaattga agcaggtgaa aatcatcagg aagccctaaa gcgtgagttg attgaagagc
      241 ttggtttcac agcagaaatt ggtacctatt acggacaagc tgacgaatat ttctattctc
      301 gtcatcgtga tacctactac tacaatcctg cctacctcta tgaagcaact tctttcaaag
      361 aagtacaaaa gccactagaa aactttaatc atattgcctg gttccctatt gacgaggcta
      421 tccaaaacct taaacgtggt agccataaat gggccattga atcttggaaa aaacagcata
      481 agattggcta atactcttcg aaaataaaat tcaaaccacg tcagcgtcgc cttaccgtac
      541 tcaagtatag cctgcggcta gcttcctagt ttgctctttg attttcattg agtataaatc
      601 aagcgacttt attcaaaaag tgctataata gaattagaaa atcggaggaa atattatgaa
      661 gcttatcaat acgacaaact cacactcgca acttgtaaaa agtcaactag aaagtaccga
      721 tgcaaccctt gttgaggttt attctgcagg gaatacagat gttattttta cccaagctcc
      781 gcttcactat gaaatcctca tctcaaacaa acaccgtgct attcgtgagc ctgaaatcga
      841 aaccattcaa gaatttttct tgaaacgtaa aattgataag acctctgtta atgaagccaa
      901 tatcaagaca ctctactcag agaaattaat tggaatttca atcccaatca agtaagaaaa
      961 ttgagtaaaa actcgatttg agagaaagtc caataagtct ttccataaca aaacgcatag
     1021 taacaagagt ttctacaccc actattatac gtttttatca ttttgaaatc tgctgctaat
     1081 caatgaagaa gcaaagatca aactaggcag ctagttacaa gttacgtaag cgcgtcataa
     1141 caaggtatct atcctaattc cccaacttcc agtatactag ataaaaaata attatcagag
     1201 gaaaggtatt gtactatgat gattgtacta agtgatttag gtcaagttca ccttgtttgt
     1261 ggcaagacag atatgaggca gggaatagac tcattagcct atgtagttaa aacccacttt
     1321 gaattggatc ctttctccgg tcaagtcttt ctcttttgtg gtg
//
LOCUS       DAVBBS010000110         1142 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVBBS010000110 DAVBBS010000000
VERSION     DAVBBS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.110"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..1122
                     /gene="rlmD"
                     /locus_tag="VQU32_002087"
     CDS             <1..1122
                     /gene="rlmD"
                     /locus_tag="VQU32_002087"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET2473469.1"
                     /translation="CGGCQIMHLHYDKQLEFKTDLLHQALKKFAPAGYENYEIRPTIG
                     MQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELKDCLVQDKETQVIANRLAELL
                     TYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNRQLNLTQLVKELVKDFPEVVT
                     VAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSLSPRAFYQLNPEQTEVLYSEA
                     VKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDIIPEAIEDAKRNAKRMGFDNT
                     HYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLLDTILTYVPEKMVYISCNVST
                     LARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKKV"
BASE COUNT          387 a          196 c          243 g          316 t
ORIGIN      
        1 tgcggaggct gccaaatcat gcacctgcat tatgataagc agctggagtt caagacggac
       61 ttacttcatc aagcgctgaa aaaatttgct cctgcaggat atgaaaatta tgaaattcgt
      121 ccaactattg gaatgcagga accaaaatat tacagagcta agttacaatt tcagactcga
      181 aaatttaaaa atcaggtcaa ggcgggctta tatgcacaaa actctcacta tttagtagag
      241 ttgaaagact gcctggtaca agataaggaa acccaagtga ttgctaatcg cttagcagaa
      301 ttacttactt atcaccagat tccaatcacg gatgagagaa aagttctagg tgtccgtact
      361 attatggtcc gacgcgcgag aaagaccgga caggttcaga ttattattgt tacaaaccgc
      421 cagcttaatt taactcaatt ggtaaaagag ttggttaaag atttcccaga agttgtgaca
      481 gtagctgtta atacaaatac agctaaaacc agtgagatat atggtgaaaa gacagagatt
      541 atctgggggc aagagagtat tcaagaaggt gtactcaatt atgaattttc actatcccct
      601 cgagcttttt atcaactaaa tcctgagcaa acagaagtcc tctatagcga agcagtaaaa
      661 gcgctggatg ttgataaaga agaccatttg attgacgctt attgtggagt tggaacgatt
      721 ggatttgcct ttgcaaagaa agtaaaaaca ctcagaggta tggatattat tccagaagct
      781 attgaagatg ccaagcgaaa tgctaaaaga atgggatttg acaatactca ttatgaagct
      841 ggaacggcag aagagattat tcctcgttgg tacaaggaag gctaccgagc agatgctttg
      901 attgttgacc caccacgtac aggtctggat gataagttat tagatactat tcttacttat
      961 gtaccagaaa aaatggttta tatttcttgt aatgtttcga ccttggctcg tgatttggta
     1021 cgcttagtag aagtctatga tcttcattat atccagtcgg tcgatatgtt cccacataca
     1081 gctcgaactg aagctgttgt aaaattaata aaaaaagttt aaaaaaggtg ttgacaaaac
     1141 at
//
LOCUS       DAVBBS010000111         1054 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVBBS010000111 DAVBBS010000000
VERSION     DAVBBS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.111"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..555
                     /locus_tag="VQU32_002088"
     CDS             <1..555
                     /locus_tag="VQU32_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2473470.1"
                     /translation="KDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGTE
                     IPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIPA
                     THNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPIS
                     NHEEMKRIISKVDLLFQKVSQLFE"
     gene            complement(503..1048)
                     /locus_tag="VQU32_002089"
     CDS             complement(503..1048)
                     /locus_tag="VQU32_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2473471.1"
                     /translation="MNWVVIKIKDIFSINTGLSYKKGDLSINNKGVRIIRGGNIKPLE
                     FSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIF
                     QLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFE
                     EQELITQKVEKLFEKVNQLWK"
BASE COUNT          380 a          165 c          153 g          356 t
ORIGIN      
        1 aaagacgaaa caacctctta tcctatatat gaaatcccag aagcatggag atatattaaa
       61 tttgctagcc tagttaactt cagaatagga aaaactcctc cacgcagtga agctactttt
      121 tggggaactg aaataccttg ggtatctata tcagatatgc ctatatctgg ttatgtaact
      181 aatacgagag aatctatttc taaattagca ttaaaatcaa agaaaataga catttcacct
      241 aaaggaactt tattaatgag ttttaaatta tctattggaa aagttgccat attggatatt
      301 cctgccactc ataatgaagc cattatatcg atattcccct atgctaataa agaaaatatt
      361 atcagagatt acttaatgat atttttgcca cttatctcta ctttaggtga ttcaaaagat
      421 gctataaaag ggaaaacatt aaatagtacc agtatctccg aattattaat ccctatttct
      481 aatcatgaag agatgaaaag aatcatttcc aaagttgatt tactttttca aaaagtttct
      541 caactttttg agtaataagt tcctgttcct caaaaggagc taacggaatt aatagctcgc
      601 ttagtgtagt tttaggaata ttatataaag cttgacctga tagtttagtt attgctttca
      661 attgtttata aaataacgga gaggacaagt taaataatag aaattttgaa ataatctctg
      721 aactttcgaa tggtgttaat tggaaaataa atccaccagc cacaacacca tcatagtctt
      781 tatcgattct tgcaaacttt ccaatatgtt ctaaagaggt tgatacaggt gttattagct
      841 gattatgttt taaataaact tgctcagagg agatgaattg tgtatcaatg tagtaatcat
      901 tatccaacag agaaaattct aaaggcttaa tattaccacc acgtataatt ctaacacctt
      961 tattattaat gcttaaatcg cccttcttgt aagaaagacc tgtatttatt gaaaaaatat
     1021 cttttatttt tataacaacc caattcatag gtat
//
LOCUS       DAVBBS010000112         1048 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVBBS010000112 DAVBBS010000000
VERSION     DAVBBS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.112"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(1..78)
                     /locus_tag="VQU32_002090"
                     /pseudo
     CDS             complement(1..78)
                     /locus_tag="VQU32_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(527..925)
                     /locus_tag="VQU32_002091"
     CDS             complement(527..925)
                     /locus_tag="VQU32_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2473472.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
BASE COUNT          315 a          197 c          158 g          378 t
ORIGIN      
        1 acgcttgatt tcagtgtgga tagtttgagg agcttttcca agtagagagg caatttctct
       61 atttgatttc cctttgttcg ccttttgtag tataatggtt ttgcatctct gtgcctttct
      121 tgtgtttgtg gttgaacaac aagtataaca caaaggtgtt ttcttatgcc tacaagagct
      181 ttcattattt ccattttctt ttggatttca ctctattctg aaaaacttgt gtatatttac
      241 tgaagctagc aagtcttacc tgtaaattta atgaaagcaa cacaaaatcc gagaggggaa
      301 tctcggatta atgtcaattt aaaaagatag agagttttta gtttaaataa attgttttaa
      361 aatatcaaca acatcacttc ttttcttaac ctgataagcc ttgattccta attttggggc
      421 tacgattata ttgtcctcaa tatcgtctag aaagacacaa tttctaggtt ataactggta
      481 tttatcgata gttactcata tatatcagtc cacctccata ctcaccttac agattctttt
      541 gataataata tctttgccta atgtagagat agtcttgcaa agaaaaaact tccttgtagc
      601 catgtttctg ataaaagtcc ggtgcctgga actggtaagt attgacaaag gcaaaacaac
      661 aatttcgatt cttagcttca ctttctgcct gttgcaatag ttttgaaccg attccttgcc
      721 ctcgcagttc ctcttttaca aacaaatact cgatttctag ccaatttcca aaagtctctg
      781 ctatcaaacc cgcaaggaga ttgccctttt catcttcgac ataaagatta agtggctcac
      841 tttcagcctc ttctcttttt gaacggttat aaacacgaat cagattccct atttcttgcg
      901 atttatgtga ttccttattt tccaatctaa agtataatga aatgaaataa aacatgcgca
      961 aatcgattaa ggaatttaat ctaatttcta acaatgtctt agaaatcaaa gtgtactatt
     1021 ttaacttcaa tgcactatac atctaata
//
LOCUS       DAVBBS010000113         1042 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVBBS010000113 DAVBBS010000000
VERSION     DAVBBS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.113"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            91..>1042
                     /locus_tag="VQU32_002092"
     CDS             91..>1042
                     /locus_tag="VQU32_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2473473.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
BASE COUNT          370 a          187 c          215 g          270 t
ORIGIN      
        1 ctgttgtcaa atgaaatgtg atttaaggta taagaaaaca cctctgtgct atacttgttg
       61 ttcaccacaa acacaaggaa aggcacagag atgcaagaac attatacccc aaaagggaaa
      121 catttgacaa tagataaccg tcgcttgatt gagcggtgga agaatgaaaa taagtccaat
      181 cgtgaaattg caggcttgtt aggaaaggcg cctcaaacga ttcataatga agtcaaaaga
      241 ggtacaactt tacaacaagt gagaaaaggg ctatacaaaa aggtctattc tgccgattac
      301 gcacaaactg tttaccaatt caatcgaaaa cggtcggtga aaaagttaat tttaacaaag
      361 gaaatcagag agaagatctt acactatcat aagcaaaaat tttcgcctga aatgatggtt
      421 aacaagaagc aagtgaaagt tggtatttca accatctact actggtttca taatggtcat
      481 ttaggattga cgaaggccga catgctttat cccagaaaaa ggaaaggtgt caagaagcaa
      541 gctagtccga actttaagcc ggcaggtaaa tctatcgaag aacgtcctga cgttattaat
      601 cttcgcttgg aaaatggtca ttatgaaatt gataccgtcc tactgactaa gataaaaaat
      661 tattgcctgt tagtcttaac cgaccggcgg agcagacacc aaattataag gttaattcca
      721 aataaaactg ctgaatctgt caatcaggcg cttacgttac tattagggga gcatcgtatt
      781 ctgtccatta ctgcagataa tggttcggag ttcaaacgat tgtctgaggt atttcctgag
      841 gaacatatct actacgcaca tgcttactct tcatgggaga gaggttcaaa tgaaaatcat
      901 aatcgattaa ttcggagatg gttacctaaa ggaaccaaga aaacgactcc gaaagaagta
      961 gcttttatcg aaaattggat taacaactac cctaaaaaat gcttggacta caagtcgcca
     1021 agtgaatttc ttttgggtgg ct
//
LOCUS       DAVBBS010000114         1035 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVBBS010000114 DAVBBS010000000
VERSION     DAVBBS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.114"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..990)
                     /gene="ltrA"
                     /locus_tag="VQU32_002093"
     CDS             complement(<1..990)
                     /gene="ltrA"
                     /locus_tag="VQU32_002093"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HET2473474.1"
                     /translation="MSKLLDKILSRDNMLEAYNQVKSNKGSAGIDGITIEEMDGYLRQ
                     NWRLTKELIKQRKYKPQPVLRVEIPKPNGGIRQLGIPTVMDRMIQQAIVQVISPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAAAKRVMYSVSRFIEKRLGLKVNMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVWRFKLKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSL"
BASE COUNT          270 a          226 c          183 g          356 t
ORIGIN      
        1 caatgagaaa tagtttatcc atcctcgaat agacaaattc agttgctcaa tacgtcttgt
       61 taggtctata ctccatttcc tctgtgttag tttcttcaat ttaagcttaa atctccaaac
      121 actatcttga tgtggacgac ttttccaacc atctgatgat ttccagaacc cgaaacctag
      181 atatttcaac tctcttggtc tggtaatctt agtcttggtc atgtttactt tcagacctag
      241 ccgtttctca ataaaacgac tgactgaata catcacacgc ttagcggctg cctcgcttcc
      301 gaccgtaatc acacaatcgt ctgcgtagcg aacaaatcga agccctctct tttctaattc
      361 cttgtccaat tcattaagca tgatattgga taagagagga gataaatttc ctccctgtgg
      421 cgtgccaact agcgttttat aacgttgacc gttaatgata acacctgaat gaagatattt
      481 acgaatcaag gattccgtat ctccgtcttc gataatgtta tgtactaagg acatcaatct
      541 atcttgagga actgtatcga aaaatttctc taggtctatg tccactatcc actcatagcc
      601 gtcatttaag tactctaaga gcttcatgat ggctttttca catgacctat ttggtctgaa
      661 tccatagctc gtatctgaga aatggggttc acaaataggg ctaataactt gaacaatggc
      721 ctgttgaatc attctatcca taactgttgg aattcctagt tggcggatgc ctccgtttgg
      781 tttgggaatc tcaactctaa gaactggttg aggcttatat tttctctgtt ttatcagttc
      841 cttagtcagg cgccagtttt gtctgagata gccatccatc tcttcgatag taattccatc
      901 aatcccagct gagcctttat tagattttac ttgattgtag gcttcaagca tattgtcgcg
      961 tgataatatt ttatctagca attttgacat gtgcctattc ctttcttgtt ttggtgcctg
     1021 agggacatct tatat
//
LOCUS       DAVBBS010000115          902 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVBBS010000115 DAVBBS010000000
VERSION     DAVBBS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.115"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            122..590
                     /locus_tag="VQU32_002094"
                     /pseudo
     CDS             122..590
                     /locus_tag="VQU32_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          331 a          135 c          166 g          270 t
ORIGIN      
        1 gagaagagga ttgccatctt ctccattttt atgtgcagag ttatagtggc ttgatgctgg
       61 gaaagtacac tgtgactgct aaaacattcc tagaagctgc tttgatttcc ctaatctatt
      121 tatgcaaatt ttatgttatt ttactataac agttgttgct aagccaaata atagtgggga
      181 agttcattta gacgtaagca ttgaagataa tcagggaggt agtgggtata atttcagttc
      241 tgtttcaagt agctcacaaa cagctaaata tgaaggaact ggttataata acaattcatc
      301 attatatata acgattgata aaacgtctga tgcaacagct cttttgaaat taaagttgaa
      361 taatgttgat aatcaacctg ctactgaagt tcctagttca ggaattactg taaaattaaa
      421 tgctaaagat aatgctggaa actggacaag tgcttcgaat aaagacaagt gcttcgaata
      481 aaaaagaaat aacagtaaaa attgtttctg ctaaaccgac atatccagac aaaatcttag
      541 tgaaaaatcc tgataatata aaagatacag aaaaaaatgc cattattgaa aaattgaaag
      601 aggcaaataa aaatcatcca gcaggagctc caacctttgc taaaggtgaa ggagagcatg
      661 caaatgatat tgtagcaact tattcagatg gtacaactta ttatgtaccg ttaaatgatg
      721 tgacaaaata tgcgaggtag tggctgtacc actcacttat tcacctcccc gtgatttgta
      781 gtagtgatag gctttctcac tattattata aaacaaaata aagatcacaa cactttttca
      841 ttctgtgttg tgccttgagt gaaacgaaag gaatgaatta taaatatgaa aagtatagtc
      901 ac
//
LOCUS       DAVBBS010000116          830 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVBBS010000116 DAVBBS010000000
VERSION     DAVBBS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.116"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            21..830
                     /locus_tag="VQU32_002095"
                     /pseudo
     CDS             21..830
                     /locus_tag="VQU32_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1515 family transposase"
BASE COUNT          266 a          174 c          162 g          228 t
ORIGIN      
        1 aaaagtgtta tactattttt atggaaacag tatacgacaa agcacaaaaa cttaactcaa
       61 aaaacttcaa actattgatt ggtgtcaaaa aggaaacctt tcaactcatg ctagaacacc
      121 tgaattcagc ctatcagatt cagcaccgaa aaggtggacg tccacgtagt ctgcccatgg
      181 aagaccagct cattatgacc ctccgttact tgcgatatta tcccactcag cgtctgctgg
      241 cctttgattt tggcgtcggt gtagctacgg taaatgccat catcacttgg gtggaggata
      301 cacttcgtgc gtcaggtagc tttgatttgg accatttaga agccccgagt gctgctgtgg
      361 ctattgacgt gaccgaaagt ccgattcagc gtccaaaaaa aaccaaagca aaaattattc
      421 tggtaaaaag aaacgacaca ccttaaaaac tcaaattatg ctggatttga cgacacataa
      481 agtctgtcaa atggcctttt ctgacggaca tacgcatgat tttactctct tcaaagaaag
      541 tattggacaa agtttgcctg aaacgacgct tgcctttgtt gacctaggtt atttaggcat
      601 cttgaaattt catgagaata ctttcattcc tgctaaaaat tccaaaaatc gccgcctgag
      661 tgaggatgat aagcagttaa ataaagagat gtcagcgata cgaattgaaa ttgaacattt
      721 taacgctaaa ttcaagacct tccaaatcat gtcagtccct tatcgtaacc gcagaaaacg
      781 tttcgagtta cgggcggaat taatttgtgc catcatcaat tatgaagtga
//
LOCUS       DAVBBS010000117          734 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVBBS010000117 DAVBBS010000000
VERSION     DAVBBS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.117"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..>734
                     /locus_tag="VQU32_002096"
     CDS             <1..>734
                     /locus_tag="VQU32_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1167A family transposase"
                     /protein_id="HET2473475.1"
                     /translation="EKISMTDIAHQLAISTSTVIRKLNDFHFEHDFSRLPKIMSWDEY
                     AFTKGKMSFIAQDFDNLNIITVLEGRTQAVIRNHFLRYDRAVRCQVKIITMDMFSPYY
                     DLAKQLFPCAKIVLDRFHIIQHLSRAMSRFRVQIMNQFERKSHEYKAIKRYWKLIQQD
                     SRKLSDKRFYRPTFRMHLTNKEILDKILSYSEDLKHHYQIYQLLLFHFQNKDPEKFFG
                     LIEDNLKQVHPLFQTVFKTFLKNKEK"
BASE COUNT          225 a          151 c          122 g          236 t
ORIGIN      
        1 gaaaaaattt ctatgactga tattgcccat cagctggcca tttcaacttc aactgtcatt
       61 cgcaagctca atgactttca ctttgagcat gatttttctc ggcttccaaa gattatgtct
      121 tgggatgagt atgccttcac taagggaaag atgagtttca ttgcgcaaga ttttgataat
      181 cttaatatta tcactgttct tgaaggcaga acacaagctg tcatccgaaa tcactttctt
      241 cgctacgata gagccgttcg ttgtcaagtg aaaatcatta cgatggatat gtttagtcct
      301 tactatgact tggctaaaca gctttttccg tgtgctaaaa tcgttctaga tcgtttccat
      361 attatccaac atctcagccg tgccatgagt cgttttcgtg ttcaaattat gaatcagttt
      421 gaacgaaaat ctcatgaata caaggctatc aagcgttact ggaaactcat ccaacaggat
      481 agtcgtaaat tgagcgataa acgtttttat cgccctactt ttcgcatgca cttaacaaat
      541 aaagaaattc ttgacaagat tttaagctat tcagaagact tgaaacacca ctatcagatc
      601 tatcaactct tactttttca ctttcagaac aaagaccctg agaaattttt cggactcatt
      661 gaggacaatc tgaagcaggt tcatcctctt tttcagactg tctttaaaac ctttctaaag
      721 aacaaagaga aaat
//
LOCUS       DAVBBS010000118          719 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVBBS010000118 DAVBBS010000000
VERSION     DAVBBS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.118"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..>719
                     /locus_tag="VQU32_002097"
     CDS             <1..>719
                     /locus_tag="VQU32_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HET2473476.1"
                     /translation="KKDAHGIALKLLSDPNREQFQHDNRQVELKILARHIHRLKKKQS
                     DWKVQYTRCLDIIFPELDKIVEKHSEYTYQLLTCYPNPQKRLEAGFDKLIEIKRLTAS
                     KIQDILSVAPRSIGTTSPAREFEIIKHYKRLIDKAETCVNDLMAEFNSVITTVTGIGN
                     RLEAVILAEIRNIHAFDNPAQLQAFAGLDSSIYQSGQIDLAGRMIKRGSPHLRWALIQ
                     AAKACPRFSPAFKAYLKTKLE"
BASE COUNT          216 a          159 c          141 g          203 t
ORIGIN      
        1 ataagaaaga cgctcatggc atcgctctta aacttctttc tgaccccaat cgtgaacaat
       61 ttcaacacga taatcggcaa gtagaactaa aaatactggc tagacatatt catcgtctca
      121 agaaaaaaca gtctgattgg aaagtacaat acactcgttg tcttgatatc atctttcctg
      181 agttggataa aatcgttgaa aagcattcag aatataccta ccaactcttg acatgctacc
      241 ctaatcctca gaaaaggctt gaggcaggat ttgataagct gatagaaatt aagcgattga
      301 ccgcttctaa aattcaggat atcctctcag ttgcacctcg ttctatcgga acaacatctc
      361 ctgctcgtga attcgaaatc atcaaacatt acaagaggct cattgacaag gcggaaacat
      421 gtgtcaatga cttgatggct gagttcaact cggtcatcac gacggtcact gggattggga
      481 atcgtttaga ggcggtcatt ttagccgaga ttcgaaatat tcatgccttt gataatcctg
      541 ctcaattaca agctttcgct ggactggatt cttctattta tcagtcaggt cagattgatt
      601 tagctggaag aatgatcaaa cggggttccc ctcatctgcg gtgggcactc atacaagctg
      661 ccaaagcatg ccctcgcttt tcacctgctt ttaaggccta tcttaagact aagttagaa
//
LOCUS       DAVBBS010000119          685 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVBBS010000119 DAVBBS010000000
VERSION     DAVBBS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.119"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(1..315)
                     /locus_tag="VQU32_002098"
                     /pseudo
     CDS             complement(1..315)
                     /locus_tag="VQU32_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(391..>685)
                     /locus_tag="VQU32_002099"
     CDS             complement(391..>685)
                     /locus_tag="VQU32_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2473477.1"
                     /translation="RKEISNWHIKASQYYLESLYKLLREKLLEQPLLHADETSYRVLE
                     SDSHLTYYWTFLSGKAENQAITLYHHDQRRSGLVVQEFLGDYSGYVHCDMLRQ"
BASE COUNT          190 a          166 c          136 g          193 t
ORIGIN      
        1 gaggctgtat tcaattgccc ttcctagttt tgaacctgct aaaactgact gacgccggca
       61 ccaagcaaag aagtcttcca ttaggggctg gagatgttct tgacgtttct gtagtcgttc
      121 atcagctggc aaagcctccc agtctctttc cagggaaaat aactgatcac aataagctaa
      181 acctttagct cctaaggatg atttatccgt ttgcttgggg ggcgcttcaa aaaacttcct
      241 tctcacatgt gcccaacatc caacaagttc agcttcttcc agttgacgat aagcttctag
      301 cggttcgcag tttaccaagc ttagcgtcgc cttgctccta aaccttggac tgctatcgca
      361 taggcagaac tagaggacta aagtcctaag ttactgccgc aacatatcac aatgcacata
      421 gccagaataa tctcctagga attcttgtac tactaaacca ctccgacgct gatcatggtg
      481 gtacagcgtg attgcttgat tctcagcttt cccagacaaa aaagtccaat agtaggtcag
      541 atggctatca ctctctagaa cccgataaga ggtttcatcc gcatgaagaa gaggttgttc
      601 taacaacttt tctcgtaaaa gcttataaag ggactctaaa tagtattgac ttgccttgat
      661 atgccaatta gagatttcct tacgt
//
LOCUS       DAVBBS010000120          611 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVBBS010000120 DAVBBS010000000
VERSION     DAVBBS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.120"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(189..>611)
                     /locus_tag="VQU32_002100"
     CDS             complement(189..>611)
                     /locus_tag="VQU32_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2473478.1"
                     /translation="KPNEEKPQTEKPEEDKEHDEVSEPTHPESDEKENHAGLNPSADN
                     LYKPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIAEAEALLEKVTDSSIRQNAVET
                     LTGLKSSLLLGTKDNNTISAEVDSLLALLKESQPTPIQ"
BASE COUNT          155 a          121 c           93 g          242 t
ORIGIN      
        1 gatgaagttt tttctcaact tcttttactt tattaaatag ttttctaaac cggttattta
       61 accatttaat taaccagtaa atagtttact atttaaaatt acttttgtca agtttctgtt
      121 acgtttttct aaaaaagaga agtaacacga aaggttactt ctccataaaa tatgctccat
      181 tcattttact actgtatagg agtcggttga ctttctttta acaaagccaa gagactatct
      241 acttctgctg aaatagtgtt attatctttc gttccgagaa gaagactact ttttagacca
      301 gtcaatgtct ccacagcatt ttgtctaata ctagaatctg ttactttttc tagcaaggcc
      361 tccgcctctg ctatcttagc gttaataaca gaatgctcta cttgaggaat ttcagcctca
      421 tctgtagtat cttcagcttc ttcctctgtc tcttccgtat cagtgcttgg tttataaaga
      481 ttatctgctg aaggatttaa accagcgtga ttctcttttt catcagattc agggtgagtt
      541 ggctcactta cttcatcatg ttccttatct tcctcaggtt tttctgtctg aggtttctcc
      601 tcgtttggtt t
//
LOCUS       DAVBBS010000121          603 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVBBS010000121 DAVBBS010000000
VERSION     DAVBBS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.121"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(1..603)
                     /locus_tag="VQU32_002101"
                     /pseudo
     CDS             complement(1..603)
                     /locus_tag="VQU32_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          194 a          104 c          129 g          176 t
ORIGIN      
        1 tgaaaaagca agagttgata gagttgatag tggtgtttca agtcttccga ataactcaaa
       61 agcttgttta aaatctcttt attggttaag tgcatacgaa aagtaggacg ataaaaacgt
      121 ttatcactca gtttacggct atcctgttga atgagtttcc agtagcgctt gatagccttg
      181 tattcatggg attttcgatg aaactgattc atgatttgga cacgcacacg actcatagca
      241 cggctaagat gttgtacaat atgaaagcga tcaagaacga ttttagcatt cgggagtgaa
      301 acagtctggg agactgtttc agcctgagcc tagaaatttg aaagcgaagc tgtttagcca
      361 agtcatagta agggctaaac atatccatag taatgatttt gacgcgacat cggacggctc
      421 tatcatattt aagaaagtga tttcgaatga tagcttgtgt tcttccctca agaacagtga
      481 tgatattgag attgttaaaa tcttgcgcaa tgaagctcat ctttcccttt gtaaaagcat
      541 actcatccca agacataatc tcaggaagac gcgaaaaatc atgctcaaag tgaaagtcat
      601 tga
//
LOCUS       DAVBBS010000122          600 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVBBS010000122 DAVBBS010000000
VERSION     DAVBBS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.122"
                     /strain="ST1"
                     /db_xref="taxon:1313"
BASE COUNT          187 a          107 c          157 g          149 t
ORIGIN      
        1 gtatatctga ggtgtaagtc ctcggtgggc acctgctacc ggtgaaccca atagcgattc
       61 ccaagcctga ctatcgtgag gtagcaggga gaggaaggga tagcgaaatc gtggctctac
      121 gaacaggaac gtgatagtaa ggcgtatata gcggataagg aggcttcaaa ctctaaagtc
      181 caaaaaggta gtcgtaacct atatgtgtaa atcacgagag taattgaatt cggactaagg
      241 tttgtgtgaa aaagataaat ctttctagag tctaaagact ctgcgtcaga tttcctattt
      301 tcactgtaac cttttaacgt cctcatatct tgtataaacg aggaaagatg tacgacttat
      361 cccgtgaggt ttcatgagcg ctgaaagcgt agtaacaacg aatcatgaga agtcagccga
      421 gcccatagta gtgaggaaac ttccgtaatg gaagtggagc gaaggggtga atactcaaac
      481 agtctgggga gagactgttt gaggtctgtc gctagaaaga gaaaacgaca gatcgaagta
      541 atcctacttc acttgtgtct gtaaaatgag tggtctgata gaactggact ttgacacgta
//
LOCUS       DAVBBS010000123          569 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVBBS010000123 DAVBBS010000000
VERSION     DAVBBS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.123"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..147
                     /locus_tag="VQU32_002102"
     CDS             <1..147
                     /locus_tag="VQU32_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2473479.1"
                     /translation="AKLEATNNLIKLIKCNAFGFRNFENFKKRIFIALNIKKERTKFV
                     LSRA"
     gene            complement(281..368)
                     /locus_tag="VQU32_002103"
     tRNA            complement(281..368)
                     /locus_tag="VQU32_002103"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(332..334),aa:Ser,seq:gct)
     gene            complement(375..448)
                     /locus_tag="VQU32_002104"
     tRNA            complement(375..448)
                     /locus_tag="VQU32_002104"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(412..414),aa:Ile,seq:gat)
     gene            complement(484..554)
                     /locus_tag="VQU32_002105"
     tRNA            complement(484..554)
                     /locus_tag="VQU32_002105"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(520..522),aa:Gly,seq:tcc)
BASE COUNT          193 a          130 c           98 g          148 t
ORIGIN      
        1 gccaaactgg aagcgaccaa taatctcatc aaacttatca agtgcaatgc ctttggtttt
       61 cgaaactttg aaaactttaa aaaacggatt tttatcgctt tgaacatcaa aaaagaaagg
      121 acgaaatttg tcctttctcg agcttagctt ttcttcaacc cactacagtt gacaaagagc
      181 cataaaaata acttcagatt agtaattcaa ttaagtttta cggatctgaa gctttattgt
      241 ctctaaaaat aaaaaagaga gttacaaact ctcattaaaa cggagaataa gggattcgaa
      301 cccttgcgcc agttacccga cctaacgatt tagcaaaccg tcctcttcag cctcttgagt
      361 aattctccaa ttaatgggca cgagtggact cgaaccaccg acctcacgct tatcaggcgt
      421 gcgctctaac cacctgagct acgcgcccaa gttaaaaaac ttggtaattt gaacaaagtt
      481 caaagcgggt gacgagaatc gaactcgcga caacagcttg gaaggctgta gttttaccac
      541 taaactacac ccgcataaat actatcaat
//
LOCUS       DAVBBS010000124          569 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVBBS010000124 DAVBBS010000000
VERSION     DAVBBS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.124"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..>569)
                     /locus_tag="VQU32_002106"
     CDS             complement(<1..>569)
                     /locus_tag="VQU32_002106"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473480.1"
                     /translation="AEALVDAEADALVEADSEAEVLAEADALVDAEADALVLAEADAL
                     VDAEADALVLAEAEALVDAEADALVLAEAEALVDAEADALVLAEADALVLAEAEALVD
                     AEAEALVDADSEALVDAEADALIDADSEADVLAEAEALVDAEAEALVDADSEALVDAE
                     AEADVDAEADALVDAEADALVDAEAEALV"
BASE COUNT          137 a          187 c          130 g          115 t
ORIGIN      
        1 tcaaccagcg cctcagcttc agcatcaacg agtgcgtcgg cttcagcatc aacgagtgca
       61 tcggcttcag catcaacgtc tgcctcagct tcagcgtcaa ccagtgcctc tgaatcggca
      121 tcaaccagtg cttcagcctc agcatcgaca agcgcttcgg cttcagcaag tacatcagct
      181 tctgaatctg catcaataag tgcgtcggct tcagcgtcaa ccagtgcctc tgaatcggca
      241 tcaaccagtg cttcagcctc agcatcaacg agtgcttcgg cttcagcaag tacaagtgcg
      301 tcagcttcag caagcacaag tgcgtcagct tcagcatcaa cgagcgcctc ggcttcagca
      361 agcaccagtg cgtcagcttc agcatcaacg agtgcctcgg cctcagcaag tacaagtgcg
      421 tcagcctcag catcaaccag tgcatcagcc tcagcaagta ccagtgcatc agcttccgca
      481 tcaacgagtg catcagcctc agcaagcacc tcagcttctg aatcggcctc aacgagtgcg
      541 tcagcctcag cgtcaacgag tgcttcggc
//
LOCUS       DAVBBS010000125          553 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVBBS010000125 DAVBBS010000000
VERSION     DAVBBS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.125"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            1..553
                     /locus_tag="VQU32_002107"
                     /pseudo
     CDS             1..553
                     /locus_tag="VQU32_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436408.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          169 a          114 c           95 g          175 t
ORIGIN      
        1 ccaattgaag aaatatgact ttcaaaaacc ttctaaaatt ccttatcttg aaacgactgg
       61 tatgcctact agaattctcc ttagaaagcg tcgtttcaag tgctatcact gttcaaaaat
      121 gatggtcgct gaaacttcta tcgtcaagaa taatcaccaa atccctcgta tcatcaacca
      181 aaagattgct caaaagttaa ttgaaaagat ttctatgact gatattgccc atcagctttc
      241 catctcaact tcaactgtta ttcgtaagct caatgacttc cactttgagt gtaattttag
      301 aaatctgcct gagattatgt cttgggacgt tgaaacagtc cggggagtga ctgtttcaat
      361 cgggagatgg agatgagctt cattgcgcaa gattttaaca atctcaatat catcactgtt
      421 cttgagggta gaacacaagc tgtcatccga gatcactttc ttaaatatga tagagccgtc
      481 cgatgtcgcg tcaaaattat tactatggat atgtttagtc cttactatga cttagctaga
      541 caacttttcc cat
//
LOCUS       DAVBBS010000126          483 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVBBS010000126 DAVBBS010000000
VERSION     DAVBBS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.126"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(155..228)
                     /locus_tag="VQU32_002108"
     tRNA            complement(155..228)
                     /locus_tag="VQU32_002108"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Asn,seq:gtt)
     gene            complement(234..349)
                     /gene="rrf"
                     /locus_tag="VQU32_002109"
     rRNA            complement(234..349)
                     /gene="rrf"
                     /locus_tag="VQU32_002109"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          149 a          111 c           77 g          146 t
ORIGIN      
        1 tcacaataag agagaacttt tttacgaaaa tctattgaat atgccataaa aagattatac
       61 cacattgtgt actatttttg gttcatttta ctataacacg aaatagatta ttattacata
      121 acaaaaaaga ggtctaaacc tcttaactaa attactccgc cagtaggact cgaacctacg
      181 acatcatgat taacagtcat gcgctactac caactgagct atggcggatt aaagctaagc
      241 gacttcccta tctcacaggg ggcaaccccc aactacttcc ggcgttctag ggcttaactt
      301 ctgtgttcgg catgggtaca ggtgtatctc ctaggctatc gtcacttaac tctgagtaat
      361 acctactcaa aattgaatat ctattcaatt caagaaaacc gttcgctttc atattctcag
      421 ttactttgga taagtcctcg agctattagt attagtccgc tacatgtgtc gccacacttc
      481 cac
//
LOCUS       DAVBBS010000127          482 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVBBS010000127 DAVBBS010000000
VERSION     DAVBBS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.127"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..>482)
                     /locus_tag="VQU32_002110"
     CDS             complement(<1..>482)
                     /locus_tag="VQU32_002110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473481.1"
                     /translation="EALVLAEADALVLAETEALVLAEAEALVDAEAEALVLAEAEALV
                     DADSDAEVLAEAEALVDAEAEALVLAEAEALVLADSDALVDAEAEALVDAEAEALVDA
                     EADALVDAEAEALVDAEAEALVDAEAEALVLAEADALVDAEAEALVLAEADALVEAEA
                     "
BASE COUNT          120 a          161 c          106 g           95 t
ORIGIN      
        1 tcagcttcgg cctcaacaag tgcgtcagct tcagcaagca ccagtgcttc ggcttcagcg
       61 tcgacaagtg cgtcagcctc agcaagcacc agtgcctcag cttcagcatc aacaagtgct
      121 tcagcctcag cgtcaacaag tgcttcagct tcagcatcaa caagtgcgtc agcttcagca
      181 tcaaccagtg cctcagcctc agcgtcgaca agtgcctcgg cttcagcatc gacaagtgca
      241 tctgaatcag caagcacaag tgcttcagct tcagcaagta ccagcgcctc agcctcagca
      301 tcaaccagtg cttcggcttc agcaagtacc tcagcgtctg aatcggcatc aacaagtgct
      361 tcagcttcag caagtacaag cgcctcagct tcagcatcaa ccagtgcttc ggcttcagca
      421 agcaccagtg cctcagtctc agcaagcacc agtgcgtcgg cttcagcaag cacaagcgcc
      481 tc
//
LOCUS       DAVBBS010000128          430 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVBBS010000128 DAVBBS010000000
VERSION     DAVBBS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.128"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            321..>430
                     /locus_tag="VQU32_002111"
     CDS             321..>430
                     /locus_tag="VQU32_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HET2473482.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVATLLEEREIV"
BASE COUNT          152 a           59 c           78 g          141 t
ORIGIN      
        1 ctaatctgga gatttttcaa tagacttcgt tattgggcgg ttacgatatg atcactgact
       61 tcgtcagtct tatctacaac ctcaaaactg tgttttgagc aacctgcggc tagcttccta
      121 gtttgctctt tgattttcat tgagtattag aacagaaaaa atacttggag tatttgtttg
      181 tgtgtttatt tttatataac aaactataaa caaaacaaaa atataaaaaa agagacaaaa
      241 aagaacagaa agcaattgac aacggtttca tatagtgtta tacttatggc ataaagttaa
      301 tggaaagaag ggaaaaactt atggtattag attatttctt tgacaaaaac cttgtgtttt
      361 gcttagaagc ggataatcaa gaacaactct ttgatcaggt tgcaacttta ttggaagaac
      421 gagaaattgt
//
LOCUS       DAVBBS010000129          393 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVBBS010000129 DAVBBS010000000
VERSION     DAVBBS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.129"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..>393
                     /locus_tag="VQU32_002112"
     CDS             <1..>393
                     /locus_tag="VQU32_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET2473483.1"
                     /translation="YLHLWELSDWDQEGMLCLHELISREEGLVDDIPRLRKYFKTKFR
                     NRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLWLDDYYLFHETLRDYRNKQSK
                     EKQEELERVLSNERFRGRQRVLRDLRIVF"
BASE COUNT          143 a           57 c           93 g          100 t
ORIGIN      
        1 taccttcatt tatgggaatt gtcggattgg gaccaagaag gcatgctctg cttacatgaa
       61 ttgattagta gagaagaagg actggtagac gatattccac gtttaaggaa atatttcaaa
      121 accaagtttc gaaatcgaat tttagactat atccgtaagc aggaaagtca gaagcgtaga
      181 tacgataaag aaccctatga agaagtggga gagatcagtc atcgtataag tgaggggggt
      241 ctctggctag atgattatta tctctttcat gaaacactaa gagattatag aaacaaacaa
      301 agtaaagaga aacaagaaga actagaacgc gtcttaagca atgaacgatt tcgagggcgt
      361 caaagagtat taagagactt acgcattgtg ttt
//
LOCUS       DAVBBS010000130          392 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVBBS010000130 DAVBBS010000000
VERSION     DAVBBS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.130"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(1..392)
                     /locus_tag="VQU32_002113"
                     /pseudo
     CDS             complement(1..392)
                     /locus_tag="VQU32_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          123 a           62 c           80 g          127 t
ORIGIN      
        1 aagggcgttg acaatctttt ctttatcttt gaggaaggtt ttaaagacag tctgaaaaat
       61 aggatgaacc tgcttaagat tgtcctcaat aagtccgaaa aatttctctg gttccttatt
      121 ctggaaatga aaaagcaaga gctgatagag ttgatagtgg tgtttcaagt cttccgaata
      181 actcaaaagc ttgtttaaaa tctctttatt ggttcaatgc atacgaaaag taggacgata
      241 aaaatgttta tcactcagtt tacgactatc ctgttgaatg agtttccagt agcgcttgat
      301 agccttgtat tcatgggatt ttcgatgaaa ctgatttatg atttgaacac gcacacgact
      361 catagcacgg ctaagatgtt gtacaatgtg aa
//
LOCUS       DAVBBS010000131          328 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVBBS010000131 DAVBBS010000000
VERSION     DAVBBS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.131"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(1..229)
                     /locus_tag="VQU32_002114"
                     /pseudo
     CDS             complement(1..229)
                     /locus_tag="VQU32_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT           99 a           70 c           52 g          107 t
ORIGIN      
        1 cagtattaca tccaccagct attaagaaaa atgattcgcc tgtatgggca tcaacagctc
       61 cataacaata gtgaaattct cgtatatagt gactatggac atgtggacct actcctattg
      121 gagaccaaca agatcccagt ttactgattc taccgaagcc agcctcatct tggtacatca
      181 agcgaacctt atgaaaacgt ctactggttt taaagcgctt tcttttcttc agttgatttc
      241 atatcactat tgtataatgc tttttgattt tagtctagta taaatagcga gaaatatcta
      301 tcctatcttc tagaatgtct tccaaacg
//
LOCUS       DAVBBS010000132          311 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVBBS010000132 DAVBBS010000000
VERSION     DAVBBS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.132"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..>311)
                     /locus_tag="VQU32_002115"
     CDS             complement(<1..>311)
                     /locus_tag="VQU32_002115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2473484.1"
                     /translation="ALVLAEADALVDAEAEALVDAEAEALVDADSEADVLAEADALVD
                     ADSDALVDAEAEALVDADAEALVLAEADALVLAEAEALVDAEAEALVLAEADALVEAE
                     A"
BASE COUNT           77 a           99 c           71 g           64 t
ORIGIN      
        1 tcagcttcgg cctcaacaag tgcgtcagct tcagcaagca ccagtgcttc ggcttcagcg
       61 tcgacaagtg cctcagcttc agcaagtacc agtgcgtcag cctcagcaag tactagcgcc
      121 tcagcatcag cgtcaacaag tgcttcggct tcagcgtcaa cgagtgcgtc tgagtcagca
      181 tcaacgagtg cgtcagcctc agcaagcaca tcagcttctg aatccgcatc aacaagtgct
      241 tcagcctcag cgtcaacaag tgcttcagct tcagcatcaa ccagtgcatc agcttcagca
      301 agtactagcg c
//
LOCUS       DAVBBS010000133          300 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVBBS010000133 DAVBBS010000000
VERSION     DAVBBS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.133"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(1..175)
                     /locus_tag="VQU32_002116"
                     /pseudo
     CDS             complement(1..175)
                     /locus_tag="VQU32_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433508.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           91 a           47 c           53 g          109 t
ORIGIN      
        1 ttgacaatct tctctttatc tttgaggaag gttttagagg atgaacctga ttcagattgt
       61 cctcaatgag tccgaaaaat ttgtcaggct ccttattctg aaagtgaaaa agcaagagtt
      121 gatagagatt atagtggtgt ttcaagtctt ctgaatagct caaaagttta tctatagtag
      181 attgaaacta gaatagtaca cctctgcttc taaaacattg ttagaaatcg atttgactgt
      241 cctgaatgat ttgtcctgtt attatttcat tttactataa atccacgttt acgaatctct
//
LOCUS       DAVBBS010000134          298 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVBBS010000134 DAVBBS010000000
VERSION     DAVBBS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.134"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(46..>298)
                     /locus_tag="VQU32_002117"
     CDS             complement(46..>298)
                     /locus_tag="VQU32_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2473485.1"
                     /translation="RKEISNWHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLE
                     SDSQLTYYWTFLSGKAEKQGITLYHHVLIDLFISYFNPL"
BASE COUNT           96 a           55 c           59 g           88 t
ORIGIN      
        1 cgtttggaag acattctaga agataggata gatatttctc actatttata atggattgaa
       61 ataagatatg aacaaatcga ttaggacatg atggtaaagc gtaatccctt gtttctcagc
      121 tttcccagac aaaaaagtcc agtagtaagt cagctgacta tcactctcta gcaccctata
      181 agaagtttca tccgcatgaa gtaagggctg agtcaatagt ctctctcgca agaggttata
      241 aaggggctcc aaatagtatt gactcgtctt gatatgccaa ttagagattt ccttacgt
//
LOCUS       DAVBBS010000135          292 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVBBS010000135 DAVBBS010000000
VERSION     DAVBBS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.135"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            51..>292
                     /locus_tag="VQU32_002118"
     rRNA            51..>292
                     /locus_tag="VQU32_002118"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           90 a           54 c           81 g           67 t
ORIGIN      
        1 gaaaaaaaca ataaatctgt cagtgacaga aatgagtgag aactcaaact tttaatgaga
       61 gtttgatcct ggctcaggac gaacgctggc ggcgtgccta atacatgcaa gtagaacgct
      121 gaaggaggag cttgcttctc tggatgagtt gcgaacgggt gagtaacgcg taggtaacct
      181 gcctggtagc gggggataac tattggaaac gatagctaat accgcataag agtagatgtt
      241 gcatgacatt tgcttaaaag gtgcacttgc atcactacca gatggacctg cg
//
LOCUS       DAVBBS010000136          288 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVBBS010000136 DAVBBS010000000
VERSION     DAVBBS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.136"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..>288
                     /locus_tag="VQU32_002119"
     CDS             <1..>288
                     /locus_tag="VQU32_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2473486.1"
                     /translation="NRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEW
                     FDEGLYEAPKGYTLEDLLATVKYYVEHPNERPHSDNGFGNASDHVQRNKNG"
BASE COUNT           93 a           59 c           59 g           77 t
ORIGIN      
        1 caaccgcgtg aaagcagcta agaaggtgcc acttgatcgt atgccttaca atcttcaata
       61 tactgtagaa gtcaaaaacg gtagtttaat catacctcat tatgaccatt accataacat
      121 caaatttgag tggtttgacg aaggccttta tgaggcacct aaggggtata ctcttgagga
      181 tcttttggcg actgtcaagt actatgtcga acatccaaac gaacgtccgc attcagataa
      241 tggttttggt aacgctagcg accatgttca aagaaacaaa aatggtca
//
LOCUS       DAVBBS010000137          278 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVBBS010000137 DAVBBS010000000
VERSION     DAVBBS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.137"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..>278
                     /locus_tag="VQU32_002120"
     CDS             <1..>278
                     /locus_tag="VQU32_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET2473487.1"
                     /translation="GVGCNWWNKRDNIAAAAVSGGMSVYQTYAIKKLIANRGMMSRVV
                     RSAITRYIGGVSMATITNALNIAATVAEVFSLGGAIAYGLDIVDGKFDG"
BASE COUNT           78 a           37 c           73 g           90 t
ORIGIN      
        1 ggtgttggtt gtaactggtg gaacaaacgt gataatattg ctgcagctgc agtttctgga
       61 gggatgtcag tataccagac atatgccatt aaaaagttaa ttgctaatcg tggtatgatg
      121 agccgagtcg ttagaagtgc tataacaaga tatattggtg gagttagtat ggcaactatt
      181 actaatgctc ttaatattgc ggctactgtt gctgaagttt tctcattagg aggagccatt
      241 gcatatggtt tagatatcgt agacggtaaa tttgatgg
//
LOCUS       DAVBBS010000138          277 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVBBS010000138 DAVBBS010000000
VERSION     DAVBBS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.138"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..>277
                     /locus_tag="VQU32_002121"
     CDS             <1..>277
                     /locus_tag="VQU32_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2473488.1"
                     /translation="QRIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAM
                     QGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQG"
BASE COUNT          126 a           37 c           47 g           67 t
ORIGIN      
        1 aacaacgcat aatagaagca atcgaatcag ctttagaaaa agtagatgaa tatgctgaaa
       61 gttataatag actagaacag ctagataaag aatttccaga taaactaaaa aaatctattc
      121 ttcaatatgc tatgcaagga aaattagttg aacaagaccc aaatgatgaa tcagtcgaag
      181 ttttacttga aaaaatacga gcagaaaaac aaaaactctt tgaagaaggc aagattaaaa
      241 agaaagattt ggacatttct attgtttccc aaggaga
//
LOCUS       DAVBBS010000139          273 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVBBS010000139 DAVBBS010000000
VERSION     DAVBBS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.139"
                     /strain="ST1"
                     /db_xref="taxon:1313"
BASE COUNT           84 a           50 c           50 g           89 t
ORIGIN      
        1 tctaaccaca gctttttgtt tcaattggta atgagtttga taacgcatag acgttttatc
       61 tcgtctaagg taacaaggca aaaagttcgt ggataaccaa gtagacttga aatactatat
      121 ctggaggtac tcccatgctt tatgtgggca ttgatgtcgc taaaaataaa cacgatgtta
      181 cagccttgaa tgttcgagga aaactgttct taaaccactc actttttcaa ataataaagc
      241 tggttttgaa ctcttagatc tgtctcttcg aca
//
LOCUS       DAVBBS010000140          233 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVBBS010000140 DAVBBS010000000
VERSION     DAVBBS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.140"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..>233)
                     /locus_tag="VQU32_002122"
     CDS             complement(<1..>233)
                     /locus_tag="VQU32_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2473489.1"
                     /translation="LLFLKPLYNLLRERLLTQPLLHADETSYRVLESHSHLTYYWTFL
                     SGKVENQAITLYHHDQSRSGSVVQEFLGDYSGYV"
BASE COUNT           71 a           50 c           52 g           60 t
ORIGIN      
        1 acatagccag aataatctcc taggaattct tgtactaccg aaccactccg actctgatca
       61 tgatggtaca gcgtgattgc ttgattctca actttcccag acaaaaaggt ccaatagtag
      121 gtcagatggc tatgactctc tagaacccga taagaagttt catccgcatg aagtaagggc
      181 tgagtcaata gtctctctcg caagaggtta taaaggggct ttaggaatag gag
//
LOCUS       DAVBBS010000141          229 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066451-rid20186923.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVBBS010000141 DAVBBS010000000
VERSION     DAVBBS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066451
            Sequence Read Archive: ERR351771
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980396.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980396.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:31:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,123
            CDSs (total)                      :: 2,083
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 61 of 133
            Pseudo Genes (incomplete)         :: 66 of 133
            Pseudo Genes (internal stop)      :: 34 of 133
            Pseudo Genes (multiple problems)  :: 25 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066451-rid20186923.denovo.141"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..>229
                     /locus_tag="VQU32_002123"
     CDS             <1..>229
                     /locus_tag="VQU32_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2473490.1"
                     /translation="YTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHS
                     HWIKKDSLSEAERAAAQAYAKEKGLTPPSTDH"
BASE COUNT           72 a           49 c           55 g           53 t
ORIGIN      
        1 tacactgatg atgagattca agtagccaag ttggcaggca agtacacaac agaagacggt
       61 tatatctttg atcctcgtga tataaccagt gatgaggggg atgcctatgt aactccacat
      121 atgacccata gccactggat taaaaaagat agtttgtctg aagctgagag agcggcagcc
      181 caggcttatg ctaaagagaa aggtttgacc cctccttcga cagaccatc
//
